LOCUS       DACDYJ010000001        99191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDYJ010000001 DACDYJ010000000
VERSION     DACDYJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 99191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 99191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.001"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            229..1092
                     /locus_tag="IWM51_000001"
     CDS             229..1092
                     /locus_tag="IWM51_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9969451.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1221..2843)
                     /locus_tag="IWM51_000002"
     CDS             complement(1221..2843)
                     /locus_tag="IWM51_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9969452.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3092..3613
                     /locus_tag="IWM51_000003"
     CDS             3092..3613
                     /locus_tag="IWM51_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969453.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3799..5529)
                     /gene="spxB"
                     /locus_tag="IWM51_000004"
     CDS             complement(3799..5529)
                     /gene="spxB"
                     /locus_tag="IWM51_000004"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP9969454.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5694..6512
                     /locus_tag="IWM51_000005"
     CDS             5694..6512
                     /locus_tag="IWM51_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP9969455.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6615..6812)
                     /locus_tag="IWM51_000006"
     CDS             complement(6615..6812)
                     /locus_tag="IWM51_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969456.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6981..7559
                     /locus_tag="IWM51_000007"
     CDS             6981..7559
                     /locus_tag="IWM51_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9969457.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8604..9107)
                     /locus_tag="IWM51_000008"
     CDS             complement(8604..9107)
                     /locus_tag="IWM51_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9969458.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9466..10491
                     /locus_tag="IWM51_000009"
     CDS             9466..10491
                     /locus_tag="IWM51_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9969459.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10814..11692
                     /locus_tag="IWM51_000010"
     CDS             10814..11692
                     /locus_tag="IWM51_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969460.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12168..12284
                     /locus_tag="IWM51_000011"
     CDS             12168..12284
                     /locus_tag="IWM51_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9969461.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13212..13550
                     /locus_tag="IWM51_000012"
     CDS             13212..13550
                     /locus_tag="IWM51_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969462.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13644..13874
                     /locus_tag="IWM51_000013"
     CDS             13644..13874
                     /locus_tag="IWM51_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969463.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14274..14645
                     /locus_tag="IWM51_000014"
     CDS             14274..14645
                     /locus_tag="IWM51_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9969464.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14919..15611)
                     /locus_tag="IWM51_000015"
     CDS             complement(14919..15611)
                     /locus_tag="IWM51_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9969465.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15614..16399)
                     /locus_tag="IWM51_000016"
     CDS             complement(15614..16399)
                     /locus_tag="IWM51_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9969466.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16636..18036
                     /gene="lacG"
                     /locus_tag="IWM51_000017"
     CDS             16636..18036
                     /gene="lacG"
                     /locus_tag="IWM51_000017"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9969467.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18061..18378
                     /locus_tag="IWM51_000018"
     CDS             18061..18378
                     /locus_tag="IWM51_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9969468.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18391..20064
                     /locus_tag="IWM51_000019"
     CDS             18391..20064
                     /locus_tag="IWM51_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9969469.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20078..21709
                     /locus_tag="IWM51_000020"
     CDS             20078..21709
                     /locus_tag="IWM51_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP9969470.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22200..23765)
                     /gene="guaA"
                     /locus_tag="IWM51_000021"
     CDS             complement(22200..23765)
                     /gene="guaA"
                     /locus_tag="IWM51_000021"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9969471.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(23824..24747)
                     /locus_tag="IWM51_000022"
     CDS             complement(23824..24747)
                     /locus_tag="IWM51_000022"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP9969472.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            24943..25548
                     /locus_tag="IWM51_000023"
     CDS             24943..25548
                     /locus_tag="IWM51_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9969473.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            25856..26872
                     /locus_tag="IWM51_000024"
     CDS             25856..26872
                     /locus_tag="IWM51_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9969474.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            27013..28314
                     /locus_tag="IWM51_000025"
     CDS             27013..28314
                     /locus_tag="IWM51_000025"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP9969475.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28360..29022
                     /gene="deoC"
                     /locus_tag="IWM51_000026"
     CDS             28360..29022
                     /gene="deoC"
                     /locus_tag="IWM51_000026"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP9969476.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            29043..29441
                     /locus_tag="IWM51_000027"
     CDS             29043..29441
                     /locus_tag="IWM51_000027"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP9969477.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29507..30589
                     /locus_tag="IWM51_000028"
     CDS             29507..30589
                     /locus_tag="IWM51_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9969478.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            30801..32363
                     /locus_tag="IWM51_000029"
     CDS             30801..32363
                     /locus_tag="IWM51_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9969479.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32356..33489
                     /locus_tag="IWM51_000030"
     CDS             32356..33489
                     /locus_tag="IWM51_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9969480.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33489..34445
                     /locus_tag="IWM51_000031"
     CDS             33489..34445
                     /locus_tag="IWM51_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9969481.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34544..35710
                     /gene="deoB"
                     /locus_tag="IWM51_000032"
     CDS             34544..35710
                     /gene="deoB"
                     /locus_tag="IWM51_000032"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP9969482.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35734..36552
                     /locus_tag="IWM51_000033"
     CDS             35734..36552
                     /locus_tag="IWM51_000033"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9969483.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36569..37276
                     /gene="deoD"
                     /locus_tag="IWM51_000034"
     CDS             36569..37276
                     /gene="deoD"
                     /locus_tag="IWM51_000034"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9969484.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(37574..38260)
                     /gene="gpmA"
                     /locus_tag="IWM51_000035"
     CDS             complement(37574..38260)
                     /gene="gpmA"
                     /locus_tag="IWM51_000035"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9969485.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(38432..39013)
                     /locus_tag="IWM51_000036"
     CDS             complement(38432..39013)
                     /locus_tag="IWM51_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9969486.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(39146..39829)
                     /gene="rpiA"
                     /locus_tag="IWM51_000037"
     CDS             complement(39146..39829)
                     /gene="rpiA"
                     /locus_tag="IWM51_000037"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP9969487.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGITTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            40148..40561
                     /gene="rpsL"
                     /locus_tag="IWM51_000038"
     CDS             40148..40561
                     /gene="rpsL"
                     /locus_tag="IWM51_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP9969488.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            40645..41115
                     /gene="rpsG"
                     /locus_tag="IWM51_000039"
     CDS             40645..41115
                     /gene="rpsG"
                     /locus_tag="IWM51_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP9969489.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            41209..43296
                     /gene="fusA"
                     /locus_tag="IWM51_000040"
     CDS             41209..43296
                     /gene="fusA"
                     /locus_tag="IWM51_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9969490.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            43468..44655
                     /gene="tuf"
                     /locus_tag="IWM51_000041"
     CDS             43468..44655
                     /gene="tuf"
                     /locus_tag="IWM51_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9969491.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            45061..45369
                     /gene="rpsJ"
                     /locus_tag="IWM51_000042"
     CDS             45061..45369
                     /gene="rpsJ"
                     /locus_tag="IWM51_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP9969492.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            45400..46029
                     /gene="rplC"
                     /locus_tag="IWM51_000043"
     CDS             45400..46029
                     /gene="rplC"
                     /locus_tag="IWM51_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP9969493.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            46057..46680
                     /gene="rplD"
                     /locus_tag="IWM51_000044"
     CDS             46057..46680
                     /gene="rplD"
                     /locus_tag="IWM51_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP9969494.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            46680..46970
                     /gene="rplW"
                     /locus_tag="IWM51_000045"
     CDS             46680..46970
                     /gene="rplW"
                     /locus_tag="IWM51_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP9969495.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            47009..47842
                     /gene="rplB"
                     /locus_tag="IWM51_000046"
     CDS             47009..47842
                     /gene="rplB"
                     /locus_tag="IWM51_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP9969496.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            47885..48163
                     /gene="rpsS"
                     /locus_tag="IWM51_000047"
     CDS             47885..48163
                     /gene="rpsS"
                     /locus_tag="IWM51_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP9969497.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            48186..48533
                     /gene="rplV"
                     /locus_tag="IWM51_000048"
     CDS             48186..48533
                     /gene="rplV"
                     /locus_tag="IWM51_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP9969498.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            48547..49203
                     /gene="rpsC"
                     /locus_tag="IWM51_000049"
     CDS             48547..49203
                     /gene="rpsC"
                     /locus_tag="IWM51_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP9969499.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            49206..49640
                     /gene="rplP"
                     /locus_tag="IWM51_000050"
     CDS             49206..49640
                     /gene="rplP"
                     /locus_tag="IWM51_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP9969500.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            49630..49818
                     /gene="rpmC"
                     /locus_tag="IWM51_000051"
     CDS             49630..49818
                     /gene="rpmC"
                     /locus_tag="IWM51_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP9969501.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            49843..50109
                     /gene="rpsQ"
                     /locus_tag="IWM51_000052"
     CDS             49843..50109
                     /gene="rpsQ"
                     /locus_tag="IWM51_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP9969502.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            50167..50535
                     /gene="rplN"
                     /locus_tag="IWM51_000053"
     CDS             50167..50535
                     /gene="rplN"
                     /locus_tag="IWM51_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP9969503.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            50572..50880
                     /gene="rplX"
                     /locus_tag="IWM51_000054"
     CDS             50572..50880
                     /gene="rplX"
                     /locus_tag="IWM51_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP9969504.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            50908..51447
                     /gene="rplE"
                     /locus_tag="IWM51_000055"
     CDS             50908..51447
                     /gene="rplE"
                     /locus_tag="IWM51_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP9969505.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            51466..51651
                     /locus_tag="IWM51_000056"
     CDS             51466..51651
                     /locus_tag="IWM51_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP9969506.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            51687..52085
                     /gene="rpsH"
                     /locus_tag="IWM51_000057"
     CDS             51687..52085
                     /gene="rpsH"
                     /locus_tag="IWM51_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP9969507.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            52117..52653
                     /gene="rplF"
                     /locus_tag="IWM51_000058"
     CDS             52117..52653
                     /gene="rplF"
                     /locus_tag="IWM51_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP9969508.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            52814..53170
                     /gene="rplR"
                     /locus_tag="IWM51_000059"
     CDS             52814..53170
                     /gene="rplR"
                     /locus_tag="IWM51_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP9969509.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            53191..53691
                     /gene="rpsE"
                     /locus_tag="IWM51_000060"
     CDS             53191..53691
                     /gene="rpsE"
                     /locus_tag="IWM51_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP9969510.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            53706..53885
                     /gene="rpmD"
                     /locus_tag="IWM51_000061"
     CDS             53706..53885
                     /gene="rpmD"
                     /locus_tag="IWM51_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP9969511.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            53929..54369
                     /gene="rplO"
                     /locus_tag="IWM51_000062"
     CDS             53929..54369
                     /gene="rplO"
                     /locus_tag="IWM51_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP9969512.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            54369..55664
                     /gene="secY"
                     /locus_tag="IWM51_000063"
     CDS             54369..55664
                     /gene="secY"
                     /locus_tag="IWM51_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP9969513.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            55726..56373
                     /locus_tag="IWM51_000064"
     CDS             55726..56373
                     /locus_tag="IWM51_000064"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP9969514.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            56564..56782
                     /gene="infA"
                     /locus_tag="IWM51_000065"
     CDS             56564..56782
                     /gene="infA"
                     /locus_tag="IWM51_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP9969515.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            56815..56931
                     /gene="rpmJ"
                     /locus_tag="IWM51_000066"
     CDS             56815..56931
                     /gene="rpmJ"
                     /locus_tag="IWM51_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP9969516.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            56949..57314
                     /gene="rpsM"
                     /locus_tag="IWM51_000067"
     CDS             56949..57314
                     /gene="rpsM"
                     /locus_tag="IWM51_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP9969517.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            57342..57731
                     /gene="rpsK"
                     /locus_tag="IWM51_000068"
     CDS             57342..57731
                     /gene="rpsK"
                     /locus_tag="IWM51_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP9969518.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            57811..58749
                     /locus_tag="IWM51_000069"
     CDS             57811..58749
                     /locus_tag="IWM51_000069"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP9969519.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            58777..59157
                     /gene="rplQ"
                     /locus_tag="IWM51_000070"
     CDS             58777..59157
                     /gene="rplQ"
                     /locus_tag="IWM51_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP9969520.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            59429..60268
                     /locus_tag="IWM51_000071"
     CDS             59429..60268
                     /locus_tag="IWM51_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9969521.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            60244..61113
                     /locus_tag="IWM51_000072"
     CDS             60244..61113
                     /locus_tag="IWM51_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9969522.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            61103..61900
                     /locus_tag="IWM51_000073"
     CDS             61103..61900
                     /locus_tag="IWM51_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9969523.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            62103..62849
                     /gene="truA"
                     /locus_tag="IWM51_000074"
     CDS             62103..62849
                     /gene="truA"
                     /locus_tag="IWM51_000074"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9969524.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(63182..65851)
                     /locus_tag="IWM51_000075"
     CDS             complement(63182..65851)
                     /locus_tag="IWM51_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9969525.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(66089..66277)
                     /locus_tag="IWM51_000076"
     CDS             complement(66089..66277)
                     /locus_tag="IWM51_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP9969526.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            66547..66909
                     /locus_tag="IWM51_000077"
     CDS             66547..66909
                     /locus_tag="IWM51_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9969527.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            66961..68643
                     /locus_tag="IWM51_000078"
     CDS             66961..68643
                     /locus_tag="IWM51_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP9969528.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            68760..70796
                     /gene="recG"
                     /locus_tag="IWM51_000079"
     CDS             68760..70796
                     /gene="recG"
                     /locus_tag="IWM51_000079"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP9969529.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            70845..71846
                     /gene="plsX"
                     /locus_tag="IWM51_000080"
     CDS             70845..71846
                     /gene="plsX"
                     /locus_tag="IWM51_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP9969530.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            71968..72210
                     /gene="acpP"
                     /locus_tag="IWM51_000081"
     CDS             71968..72210
                     /gene="acpP"
                     /locus_tag="IWM51_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9969531.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            72478..73482
                     /locus_tag="IWM51_000082"
     CDS             72478..73482
                     /locus_tag="IWM51_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9969532.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            73483..74427
                     /locus_tag="IWM51_000083"
     CDS             73483..74427
                     /locus_tag="IWM51_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9969533.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            74427..75389
                     /locus_tag="IWM51_000084"
     CDS             74427..75389
                     /locus_tag="IWM51_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9969534.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            75416..76330
                     /locus_tag="IWM51_000085"
     CDS             75416..76330
                     /locus_tag="IWM51_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9969535.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            76356..78137
                     /locus_tag="IWM51_000086"
     CDS             76356..78137
                     /locus_tag="IWM51_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9969536.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            78485..79171
                     /locus_tag="IWM51_000087"
     CDS             78485..79171
                     /locus_tag="IWM51_000087"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9969537.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            79191..82772
                     /gene="smc"
                     /locus_tag="IWM51_000088"
     CDS             79191..82772
                     /gene="smc"
                     /locus_tag="IWM51_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP9969538.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            82781..83590
                     /locus_tag="IWM51_000089"
     CDS             82781..83590
                     /locus_tag="IWM51_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9969539.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            83601..84599
                     /gene="ftsY"
                     /locus_tag="IWM51_000090"
     CDS             83601..84599
                     /gene="ftsY"
                     /locus_tag="IWM51_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP9969540.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            84786..87053
                     /gene="gshAB"
                     /locus_tag="IWM51_000091"
     CDS             84786..87053
                     /gene="gshAB"
                     /locus_tag="IWM51_000091"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP9969541.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(87142..87594)
                     /locus_tag="IWM51_000092"
     CDS             complement(87142..87594)
                     /locus_tag="IWM51_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP9969542.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            87744..88694
                     /locus_tag="IWM51_000093"
     CDS             87744..88694
                     /locus_tag="IWM51_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9969543.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            88687..89646
                     /locus_tag="IWM51_000094"
     CDS             88687..89646
                     /locus_tag="IWM51_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9969544.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            89643..90398
                     /locus_tag="IWM51_000095"
     CDS             89643..90398
                     /locus_tag="IWM51_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9969545.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            90421..91374
                     /locus_tag="IWM51_000096"
     CDS             90421..91374
                     /locus_tag="IWM51_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9969546.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(91897..92193)
                     /locus_tag="IWM51_000097"
     CDS             complement(91897..92193)
                     /locus_tag="IWM51_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP9969547.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            92548..92904
                     /locus_tag="IWM51_000098"
     CDS             92548..92904
                     /locus_tag="IWM51_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9969548.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            92914..93813
                     /locus_tag="IWM51_000099"
     CDS             92914..93813
                     /locus_tag="IWM51_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP9969549.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            94329..95060
                     /locus_tag="IWM51_000100"
     CDS             94329..95060
                     /locus_tag="IWM51_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9969550.1"
                     /translation="MMKKEIVLTAAIMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            95205..97232
                     /locus_tag="IWM51_000101"
     CDS             95205..97232
                     /locus_tag="IWM51_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP9969551.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            97340..98437
                     /locus_tag="IWM51_000102"
     CDS             97340..98437
                     /locus_tag="IWM51_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9969552.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            98744..>99191
                     /locus_tag="IWM51_000103"
     CDS             98744..>99191
                     /locus_tag="IWM51_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9969553.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        32075 a        17581 c        21089 g        28446 t
ORIGIN      
        1 ttcacggtgt tccagaagta gcttctacgg aaataagccg aaatttcaca aaaatttgag
       61 aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctct
      121 tattttgatt agaacttgcg aaaataaacc ggttaaagtg tgtctagcgc aagagatttg
      181 ctataataga cgataagggt aaaatgtaac gagaaggggt tgaaaaatgt gtatcaaatt
      241 gtaacagatt cttgttgtga tctgccatac catgtattag caaaagaaaa agttgatttt
      301 ttatcaatga atctccaatt gaatgatcaa gagttagtag atgacctcgg aaagacattc
      361 aattatgacc gctacatgaa tcagctgaaa aatggtgcga tgcctacgac atcacaaatc
      421 aatgttggcc gatatttaga atttttccgt tcttatgcgg aaaaacagat tcctgtttta
      481 tatgtggctt tttccagtgg attaagcggc tcttacagta gtgcgcttca agcggttgaa
      541 ttgctgaagg aagaatatga agaccctgag atctatgttt ttgatacaaa agctgcaagt
      601 attggggaag ggcttttagt attggaagct gtaagattga aaaaagaagg taaaagcatc
      661 gaagaaaccc tagagtggct ggaaagtaat tatttgaaag tccattcatg ggtaacagta
      721 gacgatctga aatatctaga acgaggcggt aggatttcca aagcaagtgc tactcttggc
      781 agtctattga gtgtaaagcc tattattacg gtggataaag aaggaaggct gacgaatgtt
      841 gacaaagttc gaggaagaaa caagtcgatc cagacaatcg tcaatgagac ggtaaaaaca
      901 atcgttgcac cacttgatca aatcgtttat gtggcttacg ctggggatct agagagcgcg
      961 gagaaggcaa aacggctttt ggaagagcag ataaagatga aagatgtgaa gctttatccc
     1021 ttaggaccaa cgatcgccag tcacacaggc tatggctgta tcgcgatttt ttcgatggga
     1081 atcagtcgat aaaagcagta actcattctg taaaaaagcc cagaaattga ttgtaacaaa
     1141 aacagcgact cttaaaagat agatgagcat cctatctctt agaggtcgct gtttttttat
     1201 tttttaagct aaataggcca ttatgcccat aactcgttca attgtttttg aatatctgca
     1261 ttttccaaaa attcatcata tgtcgtatca gctcgatcga tcacaccttt atccgaaacg
     1321 gcaatgatgc ggtttgccaa tgtttggata aattgatggt catgtgacgc aaataagatt
     1381 gagccagtaa atgccatcaa gccgtcattc aatgctgtga ttgattctaa gtctaaatgg
     1441 ttcgttggat catctaatac aagtacgtta gctttcgaaa gcatcatttt tgaaagcatg
     1501 caacggactt tctctccacc agataaaaca ttcactggtt tcaatacttc ttcaccagaa
     1561 aacaacattc ttcctaagaa gcttcttaag aaggtgttat catcttcttc tttacttgca
     1621 tattgacgca accagtctaa aatcgttaga tcagtatcga attctttcgt tgtatccttt
     1681 ggtaaataag aattacttgt tgtcacaccc caacgtactg ttccagtatc tggcgtaatt
     1741 tcacccatga taactttgaa tagcgttgtt gttgttatat cattttctgc gacgaaagcg
     1801 accttgtctt ctttgctcaa attaaaagat atatcatcta aaattttctt tccatcaatc
     1861 gttacactta cattttctac ctgcaataag tcgtttccaa tctcacgttc aggtttgaat
     1921 ccaacgaatg gataacgtct agaagaaggt tggatatcgt ctaaagtaat tttttcaagc
     1981 atttttttac gtgaagttgc ttgttttgat tttgatgcat tcgcactaaa gcgggcaata
     2041 aattcttgga gttctttgat ttgttcttct tttttggcat ttgcgtttgc ttgaagttta
     2101 gccgctagtt gactagattc caaccaaaaa tcataatttc ctacataaag tttgatttta
     2161 ccaaaatcta gatccgccat atgtgtacat actttgttca aaaagtgacg gtcatgggat
     2221 acaacgatca cagtattttc aaaattgatc aaaaattctt ccagccaatt gatcgattgg
     2281 atatccagac cgtttgttgg ttcgtctagc aaaagaacat caggtttacc aaatagtgtt
     2341 tgtgctaata acacttttac cttttgtcca ccagttaatt cactcatttg ctgatggtgc
     2401 aattcttcag ggatgttcag accttgcagt aaaacagctg cttctggttc agcttcccat
     2461 ccattaagct cagcaaattc tccttctaat tcagctgctc gaataccatc ttcgtcagtg
     2521 aaatcctctt tcatgtaaat cgcatctttt tctttcagca cttcatacag acgcttgtgt
     2581 cccatgatga ctgtttcaag tacagtatag tcttcatagt cgtaatggtt ctgtttcaat
     2641 acggccatcc gttcatctgg tcccatagta acggaacctg tggaaggctg cagatcccct
     2701 gataacactt taaggaacgt tgatttacct gcaccatttg caccaatcaa gccatagcag
     2761 tttccggggg taaatttaat attcacatca tcaaatagtt tgcggtctga aaagtgcaaa
     2821 ctcacatcat ttacagtaat caattttttt cctcactttt atcattatta gttttgacaa
     2881 gtcttataga attatatagg ggaaaggtaa aagattcaag gcaatcgaac attctttgtg
     2941 tttcacgtga aacagaatga aaaaaataaa aataaaagcg gagcgaaaat agctactata
     3001 ggatttatta gtttgcaaca gcataatttt tgacgttatt tataagaggg agtactataa
     3061 aactgtaaaa gaaatgaaag aaggattgaa aatggatgaa aaaagaacca tcgactgggg
     3121 ctcattgatt ttgggaattt tatttgtact tgtctcattg ttatcatttc aagaccctgt
     3181 cggaaattta gttgcaattg tagtagtctt tgcgattttt gcctttatta aagggatatt
     3241 tgaactgttt gtacggaaca gattaaaaga gctaacagga tataaaggaa agatgccctt
     3301 aataatcggg atcatcgata ttctagttgg cgtctttttc ttattcaata ttggtgcagg
     3361 agtcgcagca ttgccttttg tttttgcagt atggtttatt gctgattcag ttttagcgtt
     3421 atttactgct gacttggcaa gaggagtaag tgaaggatat tattggttta cgatcatcgt
     3481 caatatttta ggaattttat taggaatttt cttattgttt aatccaattt cttctgcgtt
     3541 gacattaagc tttttagttg gattctattt tatgttattt ggtatcacac atatcgtcta
     3601 tgcatttaga taagtaaaat caaacaaata aaaaagccgg cttccgatta cagttttact
     3661 gttgtaattg gaagccggct tttttatcat ggacaaatat gcacgaggtg atttttaggt
     3721 gtcgagacaa atttatgcaa aaatgtgaga agctatatac acaaatttta tcttaatact
     3781 cgaagcatag cgcagttgtc acaacctctt ttcatataat tcattcaata ctggcaaatc
     3841 atggacttca tatttttctt tgaatgcttg gatttcttct tcagaatatt ttcttggatc
     3901 taaatgcaga tcttctactg gcaatgggcg cttgttattg atcttcacat cgattactac
     3961 aggacgatct gattttgcag ctgcatcaaa ggcaaatcga agttgcgagt attctgtgac
     4021 tgtaaagcta tttgccccga gcgcttctcc aactttaccg aaatctgcat cttctaaaaa
     4081 tacaccaaat ttcttttgtt ccgtatcttc ttgttctgct tcgatgaaac caaaagaatc
     4141 attcgaaaag acaacattaa taattggaag ctgatatttg acttgtgtaa tgatatcttg
     4201 catgaccata gcaaacccgc catcaccact taaggtaaat acttgttttt ctgggtagct
     4261 tagttgtgcg gcaattcctc ctggcacacc attacccata gttgcgaacc atccagatgt
     4321 cgtatgtttt tgtttaccgt tcatattcaa taaacgaata gaatggatag tcgtgttacc
     4381 gacatctgtg acgaaaatcg catcgtcttc agcaatacga ttgatttctt tatagacagc
     4441 ctctggacga atcggttctt cctctcgatc ttcaaaagat ttcaaccaat ttacccattt
     4501 ttctttattt tcttgatttg ctaggtacca gctatcagca gggcgagcat cccctaactc
     4561 gacaagtctt tgcaaagctg tgtttgcatc accgaggatc gacaaagatg tttcatggcg
     4621 tcgtccaaat tttgacgcat cgatatcgat ttggataaat tcagcagttg gattaaaaaa
     4681 tgcacgtcca aatgggaagt cgctaccgac aaataagatc agatcggctt ctgccaatgc
     4741 ttcatttccg ggtttagtag cgacacgtcc agcaaaacca agaaagtttt cataatcatc
     4801 aggaataatt ccttttgcta gaacggaagc gacaacagga actgaaaaat gttctgagaa
     4861 ttctttgatt tgcggaaatc cgtttcgcgt tccttgacca acataaagaa ctggtttttt
     4921 tgctttttca agataaggca aagcagctag caaatctttt tcttctggta atatcacact
     4981 tgttttgtga gtgtgagcgg tggagaaagg ttgttcgtca atttcttcaa aaccaagatc
     5041 aacagggata gtcacgatag ccacgccttt atgctcatag gcggctttaa tggcttcatc
     5101 tactacatgc ggaagacttt gtggtgtcat gactgtacga ttgtatacgc taacatctgc
     5161 gaaaatagga ttttcattta gttcctggaa agagttatag ttcatggatg tagaagcaac
     5221 ttgtcctaag aacgctacta gaggcacatg gtccatctgt gcatcataca aaccattgat
     5281 caaatgtgtc gcaccaggtc ctgcagagcc gaagacagcg ccgactttcc ccgttaattt
     5341 tgcatctgca gcggctgcga gtgcgccgac ttcttcatga cgaacttgaa tgtagttgat
     5401 ttcggttttt tcttgatata gcgcatccat aattgaattg aaagatcctc cgggaatgcc
     5461 gtaaatatgg tcgatacccc agctttcaag aacttttacc attgcgattc ctgcattgat
     5521 ttttgacatg gtgtttcact cctttagcaa tgatgataca taaatttttt gtatctagag
     5581 tatagaactt tttatcgaaa atcccaacta attcgtcttt tcacaaaaat gtagtcatgt
     5641 ttttgggaat atgctagagt agtacataga tagattcaag agaggggaat ggcatggttg
     5701 aatggctatt gagacttgaa ccttggcagc aagcacttgc tgggacagct tttacttatt
     5761 ttatgacagc tcttggtgcc gggttggttt ttttctttaa agaaatcaaa aaagaagtgc
     5821 taaacttgat gttaggattt gcttctggtg tcatgattgc tgcaagtttt tggtctttgt
     5881 tagatccggc tattacaaaa gcagaagaaa atggagatat tgcttggcta gttgtaagta
     5941 ttgggtttgg tttaggcggt gtgtttttat atatggcaga taaaacactg ccccatatgc
     6001 attttggacc acaacatgaa gcagaaggat tgcctaccca tttaaaacgg acgatcttgc
     6061 ttgttttttc tattacattg cataacattc ctgaaggttt agccgtgggg gtagcattcg
     6121 gtgcagcagc aactgcggat aatccgacag ccgctatatt agccgctgtt tcggtggcgc
     6181 taggtattgg tatccaaaac ttcccagaag gagcagctgt atcaatcccg ttgcgacaag
     6241 aaggtctgag tcgtaaaaag gcctttgttt acggacaagc ttctggaatc gttgaaccaa
     6301 ttgctggagt gattggagcg ctactagtaa cgaaagtgga gctgctgctt ccttatgcac
     6361 ttgcttttgc cgcaggagca atgatttatg tcgtggtgga agaattgatc cctgaagcac
     6421 agcagacatt gtccagcaaa cgtcattttg cagtttttgg cgtcatgagt ggatttatca
     6481 ttatgatgat tttggatgtg gcattaggat aaataaaaaa aatatatatg atatatcaaa
     6541 tagggccgcg aacttatatc gcgaccctat ttttttagta agttatatca taatgaagtg
     6601 aatggtatca tacattaata atagtttttt ctatgtgtat ttcctttgat ccagcgaaca
     6661 agccagatga taagtgcgat tgggattaga actttgaccg caaaccataa aagcccgctt
     6721 aggacactga caaaaagact gatgacgaag ctagctgcaa ttagcactaa aagaaccatg
     6781 aagatattta agaacatatt tttttgacgc atacaaaacc ctcctcttgt tcattcttta
     6841 ctatagaaaa agtataacag ggattgttta aaaacacctc caactgaaga ctgattttta
     6901 cttcatctac ttaaatgatc aaacaattgc tttttctctg gtttatcatc taaaatagag
     6961 cggaaaagga ggagaatatc atgcctaaaa atcgtcttga agcatttaca gatgctgtaa
     7021 tcgcgattat catgactatt ttagttttag aacttcatga acctaacgga gacacgtggg
     7081 aggcgctcgc tgcattaggt taccgatttt ttatctatat tgtcagtttt atcattttag
     7141 cgatttattg gaacaatcat catcatttat ttcaagttgt acataagata aatggacggg
     7201 tcttatgggc gaacagtttc tttgtttttg cgctttcgct ttttccgttt gcaacttcat
     7261 ggatcagtga gtacatcaat agcctggctc cagaaatgac ttatggtttt gttaccttga
     7321 ccgctaatat tgcgtattat atcttggcaa aagaacttgt caaagcaaat aaaggccacg
     7381 agaaagcatt gaaggcgatg ttgaataatt acaagaaatc gtatctatcg attggtttga
     7441 atgtgttagg aattgtctta gggtatttag tagcgccagt agccgtgctg atcgcaaaca
     7501 tcgtgattct agctgcttgg atcatcccaa gtcgtaccat cgaatcacaa tatcggtaaa
     7561 atgatccgtg tttaaaattt aagagaaaaa tatagaaaaa ataaataaac gatcaaatat
     7621 ccgagtgata ctagtattca aagagggatg cagatggatc aaaaaagaat tcatgtagga
     7681 ctgattacta gttttgtcac gatgcttatt tatttggatc aaggtcattt gacttggaca
     7741 gtagttggtg tcgtaggatt tttgacatta ttcacagcaa taaaaaaata aacatcttta
     7801 tgtaatctga tgttctatct atttaagaaa agcatgttta cagcagtatg attctgttgt
     7861 aaacgtgttt ttatttgtgg aaaatttaat ttttagaaaa gtgtatcgaa aaatagatag
     7921 ttagtttttc cacagtggat aacttcgtaa gagataaaaa taaaaaatta gtcaaagaag
     7981 ggcgtataga tttcttttga taggagatat acaacagaaa tgaataaata ttaacgtgtt
     8041 ttcaaacagt ataaaacggt gtggtttttg gaataaaaaa ataacatatc tcattttaca
     8101 cttttgttat tttatactta aaaaataacg acacagaaaa taccgtgtct cagatttctc
     8161 tactacttct aaaaactact gtttttttcc tttgaaatgc cttcaaaaag taggatcagg
     8221 cagaatatac tggcagaatc aaggagaagg gagcctaaat aaggagaaaa accatagaaa
     8281 atataaagta attacttata aaacaagctt tttatagaaa aataatatac ctgagtaggg
     8341 tataaattaa gacggaattc ctttctagat tgagatattg actaggaaga atgttaagat
     8401 tttaagcctt taagctaaaa aaaaaccctt tttgttaaat aaggggaata tagcctgtca
     8461 gtttcaaact gcaagtcgtc ctgctgcaat aatatagtag attagagatg aaaatagatt
     8521 tttttaaacc aatgactgaa taaaaagacc gtcatagata cacattggaa tgtgtctatt
     8581 acggtctttt tttacgaacg attttattga agacggacag ttcgggtcaa aaataaaaaa
     8641 gctacgatca gaaataagaa taaaatgtat cccaaaataa tggctagacg gtctaactta
     8701 tttgtcgata gatgtttgtt tagaagttga taggcccata aacctatcag tcctcctgtt
     8761 gtattagtta tcaaatcagt gatgtccgct acgccaatac cgaataggaa ttgaatcaat
     8821 tctgccaaga agcttacaag gaaaataatc cctaattttt tagaaaaaga aagtctcttg
     8881 aaattgacat tcaaaagcac cccaaaagga aaaaagacaa tcaagttata agtggtctca
     8941 cgtaataccg tcgtattttc aaaagaaaaa gtagaaaaag ggacgagatt gacattgctg
     9001 taagaataag tgagaaattg tggaagatta aatgatagct tgaataaaat caaccataat
     9061 agaaagaata aataaatccc taataaaaat tttgatacat accgcataga catctcctcc
     9121 atttaaaaag tataccatcg aaaaccgctg aaaaatcata aaaaagtatg aagtttaagg
     9181 accaacgtag ttatcgtacg aaaattagaa gctttttttt gttgggaatc cggtttcttt
     9241 aacaaagcta agatagcgag ttgcttggaa aagttgctat cttttttctg tcaaaacgac
     9301 tcggaatgtt cgttttttgt tctaatttct ccaagttgtt tactttttga tactaagttt
     9361 atgaaaaatc aagaaatagt tgttatacta ggtaatgctg acaaacggga ttggcatttc
     9421 atcttggttc agcttttttt ccaattacta ggaaggaggg atagattgtt caagacttat
     9481 tttcgtttca ataaaataca agataatcct tttcgtatca tgaatctgat gctacttatc
     9541 tttattgtat tatggttagg ttacataaat catgattttt tgatttcaag tttttcatca
     9601 ttagggatct ttactttttt ctattatcaa aacataccga tgaaatattt gctgaaacgt
     9661 atgtttctga tcggttgcgg actgttggtt gctttcgttt tggggatctt atcgacttat
     9721 gttttatggc ttgaaccatt tgccgtagct ttggttgctt tttccagcag attcgtgttg
     9781 cgattattcc atatttctaa accaggtggc ttgttttttg cgatgctttc tgccatggga
     9841 acgagcatgc agctgccaat cgctcaatta ccgatcgtct cactttattt ttttatgggt
     9901 gtcgtttttg ccctgattgc ggcagtgatt acaaaattac tggattcacg gcctgagcag
     9961 acaattgaaa aagcaacttt aaaagaaaga tttcacgaag agcctttagt gatcattgat
    10021 tctgttttct acagcgctgc cttatttctt tccgtttatg tcagtcacgg cttgaatctg
    10081 cataatcctt attggttgac cttgtcatgt gcatcgattt tgctagcaga aaacttggat
    10141 gcgatgaagc atcgacaagt ccaatatctg attggatcga tgggtgggtt atgtgtgtcg
    10201 gcattcttgt catttgtgcc gtttacgcag ttgcagacca tttttctgat cacttttcta
    10261 tatggaatcg cacaatttct agttgctaga aattatgcag ttgctaatat ttttcttaat
    10321 ccgatggcgt tgatgctatc tacacttatt cgtggagcat acttgatttc tttgatcgaa
    10381 tatcgttttt tagggatcgt cataggaagt ttcatcggtt taggtgttgc gtgggtaatg
    10441 acggttggtt tgcaacatta tttaacggtt gttcgtcaaa aattagaata gaaggacaaa
    10501 tatatgtgcc aaaagaggtt atgacaagat ttttgtcaca acctctaatt caaggtgttc
    10561 aaaagccaga ttctcagcat taagtcaaat tcacacaaaa atgtggaaag gaactttcaa
    10621 aaaaatttta tatttattct atcaaaaaat tttgtttatg atggtaactg aataaacttt
    10681 cctgagatgg tcaatgcttt ccttgaaagt cttacagata ttctttcttc atagaaagtt
    10741 taagttttat agttagtgta caataaaagt atgtttatac catatggtgt ccggataatc
    10801 agtcatttaa acaatgaggt tcaaagatat gaaaagggga aatttgatga agaaaataag
    10861 caatgaaatc caaattctgg cagaaacttt aactcttccc aaagtcgtta tagagcaaat
    10921 gattgtacaa gcaaaaaaca tccttgatga ggtgtttgag caatatagtc atgaactaat
    10981 ggatcctaat aaagcaaagg cgactgcaaa gaaaatagac aaagtctatg aagaggaacc
    11041 ttttgcagca ttagctattt atctatatgc agcttctcaa agttggaaaa aaatttatca
    11101 accattgaag attccaagaa ctatctattt ggcaacaatg aatgctttta ctagatttat
    11161 tcatgaacat ttccaagtaa caggaagcta tcgctttgac cgtggttttt ggatttggca
    11221 ttacactagt ggcttgatct ttcggattgg tgaattggag tacgaaatga catattttcc
    11281 gaaagggtac acggatctaa gattagaagg aaagcagtgc ctttctcttc atattccttc
    11341 cgatgcagat ttatctcaga ataagataag agatagttac gaaagtgcca ttcgtttttt
    11401 tgaactttat tttcctgatt accattacca agtgatgtac acagatactt ggttactatc
    11461 acccaatctt actaaatggt tgaaaaaaga gtcaaaaatc tgtctttttg cagccgatta
    11521 tcgattatta tcagttgatg aacaggacga tagtggagtc ccttggatct ttggacgagt
    11581 agatgcccaa atacatgatt atccagaaag tacgagttta cagcgacaag caaaagagca
    11641 attgcttgca ggcgagcaca ttggctctgg cttaggtatt cttacttcat aagtttagta
    11701 cttcgaaata gtttaaaaga gcgtgagaca aaagtaaaga acacttttgt ctcacgctct
    11761 aaacacgtat aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa
    11821 aaatttgaaa agcaattttc gtgaatttct tcttatttct tggagttaaa cgcttctgtc
    11881 ccagcctctt ttttagtgtt ctatcgaaca cacagtacgt tcaataggta aactagctat
    11941 aacaatagat attaaagtta acggacaaga gggccttttt taggtagcta aaacgtaata
    12001 atgaaaattt atttccagag ataggagtca atcagctagt ctgatcgact gttttaacaa
    12061 gttgactttc attttaactt ttgataccat ttaaaacaca aatcgtaatt gtttcgattt
    12121 gtgtttttta gcatgactac aatcaaagaa gaaagtgggt cgtccatatg gaaaaaacag
    12181 atatctcaag tgcataccgc cgcttgaaaa gcccaaatat caaaacacga aaacgtgcgc
    12241 tgaagatcat caaagatgtg aaacggaata gtgggaaaag gtaagaagaa ggaaacttaa
    12301 ttaaaaaaat agccgaaaaa cgtttttcgg ctattttcaa taatcggttt ttgtgtaagg
    12361 tttccgaaag aactgctgtt gattttccat ttttaacata tgataaattg gtagaatctt
    12421 tcttttcaaa ttgataccat tccctaattc cagttatata gttaactaca gaaccacaaa
    12481 gcacgtacgt ttataaaagt attatctgta gttatttttc aattcccttt caacctctta
    12541 ttttgaccag tagtattgat cactttacct tttaacacct ttgttcgttt ttgaaacatt
    12601 tttagtcaag aatggaaacg taactacata atcaaaggtt ttgcgaaata tttcatgaaa
    12661 tatttcatgt agaagcgagc aatacggttg caaataacca ttatcgttta ataagcagtt
    12721 acgaaagctc ccaatatctc taacgttcag ctcactccat ttcttttctt caaaaaaatc
    12781 agtgattcgt atctctttct atttgtcgaa aaagttaact ttaaaaagta acgccgaaaa
    12841 aatatgcaaa aaaataaaaa agcagttact attgctagaa taacatcaag gaataataag
    12901 ttttggtttt cagataatag tcgataagtt gtgtattgga attttagata cagtaataaa
    12961 gtatttttca tgaaattttg tgagaatcac tattacaagt atctttaata aaattaatgg
    13021 ttaaggagga acttaaagta gaatgtaaat gaatagttgt agtcagtcga ttgtgatact
    13081 agactcaaag ttgcaggaat aagtttttca atttgtgatt ggaagataaa aaatgaaaaa
    13141 gaagggcttt tttttatact ttgttgattg tagtgtttat tttccctctg tttagccaag
    13201 taacattttc tatgactcaa ccagtttcaa gtgaggaaca tgataacctt tttcgtgcta
    13261 cagaagagtt taagaaatat caggaaaata ttgttccgga gactccatta gctagtgagc
    13321 caattactga tattgatgga aaccaaatag gatggtatat acagtacgaa gttatggtgg
    13381 taaataatga ctcaatagta aaaaatacag taggcttaga gctaacaccg attgaaaagg
    13441 tgagttcatt gttaacgtat atttatactg atgaaagtga cgagactgag atcattttaa
    13501 cggattactc aaaaattgct actgataaaa agtatatatt gtggatgtag acactagtga
    13561 aatactttat gatatagaaa ttgatgaaaa tttactagat gtagcacaag aaatcgatca
    13621 tcaaaagcag gaagctattc gatatgctgg tagaaaaggg aaaagaaaat atttattacg
    13681 tgaatgtagc aaaagttaga gaagatgaaa atgagtggaa agaattcaag agtcgctact
    13741 caattaactc gactccgaca tttacagttt acagagaagg atcgatagaa aaaactgttt
    13801 tttggactaa agaaagtgga atgtctttag ctgaagtaga agagtttttg gattatgtta
    13861 gtatgcaaca gtaagggagt tcttattatg aaaaattttg acacatatta tgttattgtt
    13921 ttttcgatat ctttgctgtt aatattacta actttaatgc agtataaaga gtatccagag
    13981 tattttattt taacttttgt atatttagta agtagctaca cacttctata taaattcaga
    14041 agttcttcta gagtttctaa agtttgtttc ttaattagct tattgattct ataatatttt
    14101 tgatttatat aggaaattaa aaatattata gaatcaacac tcagagaacg ggttggtttt
    14161 taatagattt aataagtact gataatgaaa tttctcctgg ttgggaaaac gaagttggtt
    14221 accctgtaga agaatttttt caagattact ttgcggagta tgccgatagg ataatgagag
    14281 attacgtaac agataaaact caagaagtca tcaatcaatc ccagtcatta aatgaattat
    14341 ctgataaggc gaaagaagaa gcagttgcca ttatttcttc agggattagt gactatattg
    14401 atatgagtgg tgaaatcaat tatggcgata aagacaaaat tgtacgcatg ataaaagctt
    14461 ttatcaaaac ttttgatgtg aaggcagatc ttaagtttaa tgatacttac ctagtaggaa
    14521 aattaattaa tatccttggg acagagaaag atacggaaac gtttatttcc tctttgtcta
    14581 tgttgtttac agataagcat ttttcaactc agcttggtgg agaggaacta gtggaaatct
    14641 tttaaccatt acgcccagca ttaataaaag tatatcaaaa aagtactcct gatgattttt
    14701 aagattggtt tgagaaataa tgtgaaaaca ctatttttaa tgttaattat agatagagat
    14761 gaacgcgtca tatggttaaa agaatgaaaa agtcaaagat acactgctat atttcactaa
    14821 ggatatagca gtgtatcttt catgtcgttt cactggcagt tttttataaa aggaggtgtg
    14881 catttcagtt gcttttcttt ggtagttagt tgaatcgttt aagaattaac ggatgtggca
    14941 gacaggtgct ctgtccaaaa accagtaaaa tctttgatgg ttccatcttt atttagctgt
    15001 gcctgttcaa atactgtttt gtttttaccg aatggatcaa ttgcaataat tgttccgatc
    15061 cccgcacgtt tggcagcaag taatcctgag taagcatctt cgataacaag acaatcagcc
    15121 ggagctaagc tcagtttgtc tgccgcttta agaaaaatat ccggttgcgg tttcccaggg
    15181 aaagagccgt cgtcatagac aatcttgtcc cagtcaaacc agcgggctaa atcgaaaata
    15241 tcaaaataaa aagcaacatt ctctttgaca gtagccgtgg caatcgttaa tggaatctgt
    15301 tggtcagtca gctggtcaag agtcgcttca agcccttttg tcaactgaag ctcgttttca
    15361 ttttgcaagc aaagcatacg ataatagctt tctttttctt ctgacaatac ggcgatctca
    15421 gaggcggtca aatgctctga aatgaaatgt ttcaaaatct ccgtattcgt ccgaccatga
    15481 atatttttta aaatatcctc gtctgataaa ccatcttttg tatgatcatg tatcatatgc
    15541 agccaagcag cttcatgcag atgagagtcc aaaaacattg ttccgttgaa atcaaatata
    15601 atccctttca tagttacgct cctatagtgg tattttcggt tgaatcagca atttgcagat
    15661 cgacatattg tgcatagtgc tcttgaacct cccgcgagat ctggcgatcc gtaatgagca
    15721 gatcgaaatc atgcaaatta taaaacacat aaaaatcatc tcgattgaat tttttattat
    15781 caacgagtag atatttctta cgggaattat ttagagcgat ctgctgtgct tctccttctt
    15841 cggtactgta agtagagatc gcttcgttgt gaacgccatt tgaactgata aatgcttttg
    15901 taaactttag tcttcttaaa ttgtcgttgg tgatcggtcc gacaaatgcg ccggtatttt
    15961 tccggaaatc accgccaatc agtagaattt ctggtccatc tgcttgagca agaatatcaa
    16021 aaactggata attatttgta atgatccgaa ttttcttgtt cagtaaatgc gtcgccaata
    16081 gttcgattgt tgtacctggt cctaagaaaa tcgtttctcc ctcgtgaatc aaggaggctg
    16141 ctgtggcagc aatttctttt ttttcttgaa tctgcagagt ggctttttca gtgtgagaaa
    16201 gttcttgatc taaagtgtag gaaagacttt gagcccctcc atggacacgc agcagcttgc
    16261 ccgatttttc taattcatct aaatctctgc gaacggtcat atcagaaaca tctaattgat
    16321 tgatgatttc attaacggta aggattcctt ttttattgac catttctacg atagtaagca
    16381 atctttcttt tttcaacatt gtcttcacct ctaatttaag gataatctat catataaacg
    16441 gacaacagtc aatctaattc gaacaaaagt aaacatttag ggtggttttt atgtttgtta
    16501 caatagattt tattgctgtt ttctggtttt tatgattatt tgtgtttgtt tttgttgaca
    16561 aatgttttgg atgcgtttac aatgcaatca gttagaaaga gcgaaaactg attatagatt
    16621 tgaggagaga tgtacatgct taaattacca gaagatttta tttttggcgg agccacagca
    16681 gcctatcagg ttgaaggagc cactaaggaa ggtggcaaag gcgctgttgc ttgggatgac
    16741 tttttagagg aacaagggcg attcagtccc gatccggcaa gtgattttta tcatcaatat
    16801 gcaaaggaca ttgaattgtg tgaacgtttt ggtgtaaatg gtctaagatt atccattgct
    16861 tggagccgca tttttcctga tggagctggg aaaccaaatc cagaaggaat cgctttttat
    16921 catcgtgtgt tcgaagaatg taagaaaaga aacgtaacgc cgtttgtgac gttgcatcat
    16981 tttgataccc ctaaacgttt gtttgaccat ggagattttt tgaatcgaga aacaatagaa
    17041 gcatttgttt cctatgccat tttttgtttt catgaattca aagaagtcaa ggtgtggagt
    17101 acctttaatg aaatctatcc agttgcaacc aatcaatatt tgttaggtgt tttcccacca
    17161 ggaatcaaat atgattttac aaagatcgtc gcctgtcttc ataatatgat ggttgctcat
    17221 gcccgtgtag tgaactattt caaagaaaac gaacttccgg gtgaaattgg tgtagttcac
    17281 tcattagaga cgaaatatgc ggcaacagac gcgccggaag ataagcatgc tgccttttta
    17341 gacgatgcat tgtcgatccg tttcttatta gatgctacct atttaggcta ttattcaact
    17401 gaaaccttga ctgctcttga tgagatatgt gaagcaaatc aggccagcta tcattttcct
    17461 gaagaagatt tcgttgaatt gaaaaaagca tccacgcgta atgactactt agggatcaat
    17521 cactaccaat gccattttgt caaagcttac gatggagaaa atgcaatcca tcataacggt
    17581 acgggtgaaa aagggacctc tgtttataaa gtgaagggaa tcggagaacg tatttataaa
    17641 gaaggaattc ctcgaactga ttgggactgg ctgatttatc cagaaggtct atatgacttg
    17701 ttgctgcgaa ttaaaagcga ttacccacat tacaacaaaa tctatatcac tgagaacggg
    17761 atggggtata aagatcagtt tgaagatggc attattatgg atcagccgag aatcgactat
    17821 ttgagagtct atttggaatc cttaagcaag gcgataacag ccggggtaaa tgtgaagggg
    17881 tattttttgt ggtcgcttat ggatcttttc tcatggacaa atgggtataa caaaaggtat
    17941 ggcttgtttt atgttgattt tgaaacccag aagcgttacc caaaagaatc agcttactgg
    18001 tacaagttgg tcagtgaaac caaaacgatt atttaataag tgataatgga ggaagaaaag
    18061 atgaataaag aggaattaca aatgcttggt tttgagatcg ttgcatattc aggcgacgct
    18121 cgcagtacat tattgaaatt attgaaagaa gtaagacaag gaaactttga acatgttgat
    18181 tcggcgttga aagaagccga cgaaaattta acccttgcac ataattcgca aacaaaaatt
    18241 ttagcggaag aagcatctgg gaaagagatg gagatgggat tcatttttat ccatgggcag
    18301 gatcatttaa tgaccacttt attgctgaga gatctgattc aagactttat tgtactgtac
    18361 agacagaatc aaggatagga gaagaacgct atggatggaa tcattaaaca aatcgaaaaa
    18421 gggaaaccat tttttgaaaa aatctcacgt aatagttatt taggagccgt tcgtgacgga
    18481 tttttaacag cgatgccagc tattttattt tcaagcattt tcattttgat tgctgcggtt
    18541 cctgagattt ttggttacca atggcctgaa tctgtctcca catggctatg gaaagtctat
    18601 aattattcga tgggtgttgt agggttactc gtttcagcaa ccactgctcg ctgcttggct
    18661 gattcgatga atcgtcgctt accgaaaaac aaagtaatca acaccacctc tgtgatgctg
    18721 gcctctgtgg taagtttcct gttgttaagc gtctctccga ttgaaggtgg attttcaacg
    18781 gaatatatgg ggacaaaggg actactggct tcctttgtcg ctgcattttt aacagttaat
    18841 ttatataagt tttgtgtgct gaaagatatt accattaaaa tgcctaaaga agttcctgga
    18901 acgatttcgc aaaccttccg agatattttt cctttctctt tttcagtatt tgcggccgtt
    18961 ttaattgatt taggcattcg acaaatcttc caaatctcat ttgcagaagc aatcattacg
    19021 gtgctgcagc cgctatttac agcagctgac gggtacttag gaatcgctat tatctgggga
    19081 gcgatggcgc tgttttggtt tgtcggtgtt catggaccgt cgatcgtgga gccggcaatt
    19141 gcggcaatta tctatgccaa tgtggaaacc aatctgcaat tgttcaattc cggtgaacaa
    19201 gcttctaaag tactgacggt gggtttaggg aattttgtag gaactatggg aggaactggg
    19261 gcaacattgg ttgttccttt tatcttccta ctctttgcta agtctaagca gttgaaggca
    19321 gttgggaaag cttcctttat tccagtaagt ttcgctgtca acgaaccatt gctatttgcg
    19381 gcacccatga ttttgaaccc ttattttttt attccatttt tgttaacacc tgttgtaaac
    19441 gtttgcctgt tcaagttttt tgtcgatgta ttgggcatga acagctttat gtacgttttg
    19501 ccttgggcaa ctccagcacc gatcggtttg gttctaggaa cgggcatggg tatcttagct
    19561 tttgttctag cagcggttct aatcgtcgtt gacttcctga tctatctacc attttgcaaa
    19621 gcatacgatg ctgtcttagt agagaaagaa gcacaaaaag cagaggtgat cgcaaccgag
    19681 gaaggtgctt ctgccgatgt acgtgaggaa gtacctgtgg cagcgagtca agttatcagc
    19741 gatgcaaata aagaaatcaa tgtgttggta ctttgtgctg gcgcagggac aagtgcgatg
    19801 ttagctaatg ctttaaaaaa aggtgcatct gaatcgaaag caccgatcag tgcaatggcg
    19861 ggtgcttatg gttctcatta cgaaatgctg caggattttg atatggtggt cttagcaccg
    19921 caagtcaatt cttactatga tgatatcaaa gaagacaccg atcgcttagg aatcaagctg
    19981 gttgcgacaa aaggagcaga atatatcgga ttaacgagag atcctgaaaa agctgttcag
    20041 tttattctga accaatttca ataaaggagg agccataatg aaaatgggaa tttggttgcg
    20101 taaagctgtt ttatttgcag ctgtgttagg ggctttgggg gctgtttcaa aaactgttga
    20161 agcatctgcc agcgaattta ttcgcggagc agatatttcg atcctgtcag atatggaaaa
    20221 atctggtgca aaatattatg aagacgatat ttcaaaagat gccttagaga ttttaaaaaa
    20281 taatggtgtc aactatgttc gactgcgttt gtggcaggat ccttataata gtaatggaga
    20341 aagttatgga gcagggacaa atgatctgga aacgacgatt gaccttgcaa aacgagcaaa
    20401 aaatctggga cttaaggtgc tgctggattt tcactacagt gatttttggg ttgatcctgg
    20461 gaaacagaat cttcctaaag catggcaagg tctgaccttt gaagaaatga atacggcatt
    20521 gtacgactat acaaagaacg ttctgtcgga aatgaagcag ctggacgttt atcctgatat
    20581 ggttcagatc ggcaatgaac tgaatagcgg aatgctctgg ccttatggca agtcttgggg
    20641 agaaggcggg ggagaatttg atcgattggc tgcttttttg aagtctggta tccaagctgt
    20701 tcgagacact caacctaaaa caactccagt aatgctccac ttagctgatg gtggcgatac
    20761 aggagcattt acttggtggt ttgatgaaat cacgagtcga ggtgtgtcat ttgatttaat
    20821 tggagtgtcg tattatccgt attggcacgg ttcaatgaat gaacttcaaa ccaatatgaa
    20881 tgcgatcagc caacggtatg gtaaagaagt gatcgtagtt gaaacagctt atgggcatac
    20941 aacagcgaat gccgatacta tgccaaatgc ttttggtgaa gccgaagcag ctgcgggggg
    21001 atatgaacca acaccgacag gacaagcaga gtatttgctg gatcttgccg atcggattca
    21061 ggcagtaccg aataatcgcg gggcgggctt cttctattgg gaacctcttt ggtacaacgg
    21121 aaatgtgagt tgggcaacac aagcaggtat gaattatctc agtgttcaat ccactatggg
    21181 aaacgaatgg gacaaccaag cgatgtttga ttttgctgga aacgccttgc caagcttgcg
    21241 tgcgtttaaa caagcagggg cgcaaataaa tctagttaaa aatgcaagtt tcgaaagcga
    21301 tggaccaacg accagtcctt ccaattggca aacctggttt caacaaggaa ccgctaaggg
    21361 tacgatcaaa acggaagcgg gcactttttt tggggactac aaactaactt tttggcagga
    21421 tcaagcatac gaggcatcag cttatcaaaa tgttagtggc ataagcaaag gaacttacac
    21481 attgagtgcc tatgtaatgt ctgggggaga gtttgatact aatcagttat atatcaaaaa
    21541 ttatggtgga gcggaactaa atactgcggt tgttaaaaca agtgaatgga caaaaattac
    21601 aatcaaagac attccaatta caacaggtgt ttgtgaaatt ggggtttata gcaaggcttc
    21661 aggaaatgct tggacgaacg ttgattttgt ttcattggtg aaacaataga ctgcggatcg
    21721 tttcgattca agatcggagg ttcccttcag ccacatcgtt agcgtagaag gcaggaatcg
    21781 aacctgcacc tcgcctttgc caaaaataac cagaaacgat ttccagtcat ttttaccaaa
    21841 acgagtaaag caccagactt catctacact ttgtattatg ctaaagagag ttcaacgatt
    21901 tcgtaagaag caatgaaaaa attgcaaaaa gttgtgcaga taacataaag tttgtttaag
    21961 aaagggtaac cttagaattc cgtcgtgaat atgatttgag cacttagatg gatagaaaaa
    22021 agttctcgga aaacagttcg tttttcgaga acttcttttt atgataagaa tgaaacaaat
    22081 taacttatat tatagtctct tttggtaggg aaccatctta tttttaatga aaatcaagca
    22141 ttttagctat tgaagaatgt tgatttatag ggattccccc tccatattat gttggctctt
    22201 tattcccact ccactgttgc tggtggttta gaagtgatgt catacacgat acggttgacg
    22261 tgggctactt cgtttacgat acgtacagag attttttgca agacttccca tgggatacgg
    22321 gcaaagtcag cggtcattcc gtcgattgaa gttacagcac ggataccgac tgtgtagtcg
    22381 taagtacgtc cgtcacccat aacaccgaca gagcggatcc ctggaagaac agtgaaatat
    22441 tgccagatat cacggtcaag tccagcagct gcgatttctt cacgcaagat agcatcagat
    22501 tcgcgtacga tttctagctt gtcttcagtg atttcgccaa gaacacgaat accaagacca
    22561 ggaccaggga atggctgacg ccaaacgatt gaatcaggca tgcctaactg agtccctaat
    22621 tcacggactt cgtctttgaa taatgtgttt aatggttcga tcaattcaaa ttgcatatct
    22681 tctggcagtc cacctacatt atggtgagat ttgatcgtct gtgctgtttc tgtaccagat
    22741 tcgataacgt cagtatacaa cgttccttgt gctaggaaag agacgccttc ttcaccagca
    22801 agttttgttg cttcgtcatc aaaaacatat acgaattcat tcccaatgat tttacgtttt
    22861 tgttctgggt cagatacacc tttcaacttg tctaagaagc gatctcttgc attgactttg
    22921 atgatgttca agccgaattt tccgcctaga ctttccatta cttgttccgc ttcgcctttg
    22981 cgtagcagac cgtggtcaac aaagatagat gtcaattggt caccgatcgc tttttgtaaa
    23041 agaaccccaa cgacactaga atcaacgcca cctgaaagac caagcaagac ttttttgtca
    23101 ccaacttgtt cacggatttt ttctacttgc atatcgataa agttatccat tgtccagtta
    23161 ccttcacagt gacaaacatc aaaggcaaag tggcgcagca attcgattcc gtattcggaa
    23221 tgacgtactt ctgcatggaa ttggatacca taaaaattac gtgttgtgtc ttggatagaa
    23281 gcaatcggac aatctttgct tgttccgact gtttcaaagc cttcaggaac tttagttacc
    23341 aagtcaccat ggctcatcca tactgtttgt tgttttggtg tttctttaaa caagacagct
    23401 tgatcgcttg ttacttctaa caaggctttc ccatattcgc ggtttttagc aggctctacg
    23461 gaaccgccaa gtttgtacgt gatcaattgc ataccatagc aaatccctaa tacaggaata
    23521 cctaaattga agatctcagg atcgatccca aatgcattct catcatatac actgtttggt
    23581 ccgccagaaa gaatgattcc ctttggattc atcgctttta cttcttctgc agtaatgcgg
    23641 tggctcaata attcggaaaa gactccaaga tcacgaatac gtcttgtgat caattggtta
    23701 tactgactac cgtagtctaa tacgataatc ttttcaacgt tcgtcatatc ggcaacgttt
    23761 gtcaaattta ttacccctat ccctttaaat tttatctcaa aatagtcaaa ctattttaaa
    23821 atgctaatat tttcgcatga aaatcgaatc gatgatatga ttttcagttt tatggagaat
    23881 tatatcggct cttcctcgtg taggcaagat atattcctgt aaatttttca aattcacatt
    23941 tttccataca tcttttgcca ttttaaaggc ttcttcacga tctccgatag caaaacgata
    24001 ataataattc tctggatttt ggaaagccgt atccagtaac gcaccaaaac gatccaaata
    24061 ccatttttcg atcagcacgg gatcggcatc gacatagaca gaaaaatcaa aaaagtcgct
    24121 gacgtagatt tgctgattcg caggtaattg aagcgtattg atcccctcaa cgatcaagat
    24181 atcaggctgc tggatcaatt cgtacttgtc tggaatgata tcatacacat catgtgagta
    24241 aacaggtact tttatctctt cctgaccaga ttttacacgg ttcaagaaat cgatcagcat
    24301 ctccatatca taactttcag gaaatccttt acggtccatg attccacgct cttttaatat
    24361 acgatttgga taaagaaatc cgtcagtcgt gatcagctga acgtttcttc gtttgaaggt
    24421 acgagacaaa atcgtctgca gcagtctggc tgttgtactt tttccaacgg ctacacttcc
    24481 ggcgatcccg ataataaaag gcggtacttc tacgtattta tgaagaaata aacctttact
    24541 caagaacaaa gattcaaatt ctttcatata aagataaatc agatgagcaa gaggtacata
    24601 gatatctcga acatcctgca tggaaatgcg gtcattcaaa cttttgatat tatctagttc
    24661 atcttgtgtc aaaggtgctt gcccattacg ataaaattct cgccattctt ctcttgccac
    24721 acggtagtag ttcattggat cattcatatt agcctcctgc gttctcccat caatggaact
    24781 attttaacac aaaataaacg caaaccccac cacttaagag agattgccga tctttttgta
    24841 taatctttat aatagaaaga aacggagtct tattcttgac ttgcgcacgg atacataaaa
    24901 ggatagactg ttgtcatgaa agaaatgaga aggatggaag acatgacaaa tcattactat
    24961 tctgaacgcc cagatacggc gcacgatttt gaacaatggg cttttgagtt aaaaggcaga
    25021 acctttcaat ttgtaacaga ttctggtgta ttttcacgtg aaacagtgga ttatggttca
    25081 cgtgtcctga ttgatgcttt cgattgggaa gagttacctg atgaaggaaa gatcttagat
    25141 gtgggatgcg ggtatggacc gattgggtta gctttggcat ttgcgacaca gcggtttgtt
    25201 gaaatggtcg acattaatga acgagcggta agtctagcgc aaggaaacgc aaagcgtaac
    25261 caaatcgaac atgttgatat ccatcaatcg aatatctatg aagcagttca tgaagaaacc
    25321 tatgcagcta tcgtaagcaa tccgccgatc cgtgctggaa aaaaagttgt acatgaaatt
    25381 ttgacaggcg catatcctcg gttgaagaag ggcggtacac tgacaattgt gatccagaag
    25441 aaacaaggag cgccgagtgc gcaaaagaaa atggaagaaa catttggtaa tgcagaaatc
    25501 gtcacaaaag ataaaggata ttatatcatc aaaagtgtga aagaataact agctgaaaaa
    25561 tagtggagcg acgaaagagt ggaactgggc gtgcatgtac gggtacgtat cagttctttt
    25621 tttgttgaga agtttttaaa aacaaagaac tattttttct tcaaaaagaa atagtaggaa
    25681 cagagtcgtt gttttttacg ataaaataaa taagatacag aacttgtata gaaggctgcc
    25741 aagtcggtag ataaaggagg tttttatcaa aaaacatact ttcctttttt ctctattctt
    25801 ttcttggggg ttttggtaca ctgataggga atttagatag aagggagcca ataggatgaa
    25861 gctgaccatc agagatatcg cagaaatggc aggcgtatca gtaacaactg tctcacagat
    25921 tttgaataac aaaggaagca gatttagtga taagacaaga aaaaaagtgt tgtcgatcgt
    25981 gaatgaacat cattataagc ccgattattt cgcttcaaat gtgatcaatc gacattcaaa
    26041 gaccatcgga atgatcgtcc ctgatgtcac tgatttcttc ttctccaaac tgatcgaagg
    26101 tgtagagagc tatttgaatc ctttgggcta tgtgatcatg ttatgcaatt ctcgtcatag
    26161 ccaagaaaat gagatcaagt atttacaaga gctttcccac cgctcagtgg acggaatctt
    26221 gctggccaca ccaaatattt tacctgagaa atatgcatta gacagcggtt tttatcaaaa
    26281 aatgccaatc attttaatcg atcgcggatt gaatcgtcgg gataacggac ggcttatcgt
    26341 aaaggaatat gagggcgcct atcaagctgt ttcgctgttt attgaaaatg gccatacgaa
    26401 gatcggcatg ctgaaggaaa ccactggata ctatcaatta gaagaacgtt tcaacggata
    26461 tcgtcatgcg ctgaaagatc atggcctgcc ttttaatggg aaatttgttg aacatggaga
    26521 gttgactgtc caaggtggat atactgcatc aaaaaaatta ctcaaacata aagatatcac
    26581 ggctattttt tgcgggaatg atgccatggc gatcggatgc tatcaggcga tcgatgaatt
    26641 gggcaaaaaa atcccagaag atatctcagt cataggattc gatggattaa agctgtcaga
    26701 atacatgatt ccgcaattga cgacagtgca gcagccaagt tttgatattg gattttatgc
    26761 tgcacgtttc ctgatcgata cgatcgaatt tcctcaaaga aaagttccga acaaagtgtt
    26821 cgaaacaaaa ttaattattc gtgagagcgt aaaagtattg acaaagggct aactcggcgg
    26881 tatattgctt atgtaagcat ttacaaaggc tagttagtgc tttttttatt tttagtaaac
    26941 cagtttacta aagtggttta cctttcctaa aaataatgta taataccgaa tgaagacaag
    27001 gaggatcagg caatgcgaat ggtggatctg attgaaaaaa agagggatgg aaacgagctg
    27061 tcgaaagagg aaatcgagta catcgtcacg aactatacga atggaaagat tccagattat
    27121 caagtcagtg cattattgat ggcaattttt tatcaagaca tgacaaatga agaaatcacg
    27181 aacttaacat tggcgattgc taattctggt gatgtcatcg atttgagttc tcttgaagga
    27241 ataaaggtag ataagcattc gacaggtggc gttggtgaca cgaccacatt gattttagcc
    27301 cccctagtag ctagtgtcgg agtgacagtg gctaaaatgt ctggacgcgg tctaggttac
    27361 acaggtggta cactagacaa gctagaagca attcccggat ttcagatcga gctttcggat
    27421 gaagaatttg tacgcattgt taatgaaagt aaagtagcgg taattggtca atcaggaaac
    27481 ttggcaccgg cggacaaaaa attgtatgcc ttgcgtgacg tcacagcgac cgttgattct
    27541 cttccgctga ttgccagttc gattatgagt aaaaaaatcg cggcaggagc agatgcaatc
    27601 gttttggatg tgaccactgg agacggtgct ttcatgaaga acatcgaaga tgcaagacgt
    27661 ctggcaaaga ccatgacgag cattgggaaa ctggctaacc gagaaacagt ggcggttatc
    27721 tcagatatgt cagaacctct tggcgaagct atcggaaata gtctagaagt tgtcgaggca
    27781 atcgaaacat tacaaggaaa tggtccagaa gatctagtag aaatgtgcta cgccttagga
    27841 agccaaatgg ttgttcttgc tggcaaagct aagacaaccg atgaagcaag aacgttgctt
    27901 caagaagctc tggaatcagg taaagcgtta gcaaaattca aagaaatgat ccaaaatcaa
    27961 gggggagacc cagcgatcgt ggaacatcca gaacgaattt tgactgcacg ctacacaatg
    28021 gagcttcctg ctaaacaaag cggagttgtg tcaaaaatcg tggcaaatga attaggaatt
    28081 gctgcaatga tgctaggtgc tgggcgtaaa accaaagaag aagatattga tcacgctgtt
    28141 ggattgaaac tgcataaaaa aattggtgac actgtgacaa aaggtgagtc actattgacg
    28201 atctatagta acgacgaaga aatcacttct gtcattgaat tattatataa aaatattgaa
    28261 attggtgaat cggcaatgaa gccaacgttg attcatgaca tcatcacaga ataaaggaaa
    28321 aaagcggact ttaccgtttt aaagaaaagg ggaaaagaaa tggaactaaa ccgaatgatc
    28381 gaccatacta ttttgaaagc agacgcatct aaagaagctg tgatgcaaat tatcgaagaa
    28441 gcaaaaaaat accattttta ttctgtctgc atcaatccag catgggttgc gctagcgaaa
    28501 gaacaattac aaggtgagcc agtagccgta tgtacagtaa tcgggtttcc tctaggagca
    28561 aacacacccg agacaaaagc atttgaaaca acagatgcca tcaataacgg tgcagatgaa
    28621 gtcgatatgg tcatcaatat cggtgctttg aaatcaaagg atgatcaaca agtccaaaaa
    28681 gatatcgaag cagttgttga agcagcaaaa gatcgtgcgc ttgtcaaagt tattattgaa
    28741 acatcgttat tagatagaga agaaatcatc cgagcttgcg aaatagcaaa agctgcaggc
    28801 gctgattttg tcaaaacatc aactggtttc tcaacaggcg gagcaaaggt agaggatgtc
    28861 cgtttgatgc gtgaaactgt aggaccagaa atgggtgtca aagcttccgg cggtatccat
    28921 aacgaagaag aagcaatggc aatgatcgaa gctggtgcta caagaatcgg aacaagcgca
    28981 ggagtagcaa tcgtatctgg ttcaactggt gaaggctatt aattttttca aaggagaagc
    29041 tcatggcaga actcaaacaa gaatggatca acacagctat tgaagcgtta gataaagcct
    29101 atgttcctta ttcccatttt ccggtaggcg cttgtctcgt aacggaatca ggcaagattt
    29161 atcaaggcat caacattgag aatgcatcat ttggattaac gaattgtgcc gagcgcacag
    29221 cttttttcaa agcagtatct gaaggagaaa aaacatttac gcatctagtc gtagcggggc
    29281 acacccccga tcctatctct ccttgtggtg catgtcgaca agtaatggca gaattctgtt
    29341 cccctgatat gccggttacg ctagtaggag acaatggtgt gacgaaggca acgaccgttc
    29401 gtgcactgct gccttatgca tttactgaaa aagatctata actcatgttc attcagtcag
    29461 ctgactgttg aaaaataaaa caattatttt aggaggcttt acagaaatga aaaaagcaaa
    29521 attatttggt ttaggtgctg tcgcactagc agcagggtta tttttaggtg catgcggcaa
    29581 caacggctct actgattcat ccggtggcaa aagctcatca gacacaacaa ctgcagcatt
    29641 gatcacagac acaggtggtg tagacgaccg ttcattcaac cagtcagctt gggaaggact
    29701 agaaaaatgg gggaaagatc aaggactttc acgaggaaat gatggcttcc aatatttcca
    29761 atcttcaaat gaatcagatt acatcccaaa tattgaccaa gcattgaatg ctggattcaa
    29821 aacgattttt ggtattggtt ataaactaaa accagcgatc caagaacaag caacaaacaa
    29881 tacaggtacg aactttgtta ttatcgatga tgtcatcgat ggattagata acgttgtatc
    29941 tgcaactttc aaagacaacg aagcttctta tcttgcaggt gttgcagcag cttatacgac
    30001 tgaaacaaac gtagtcggct ttatcggcgg tgtaaaaggt gaagtaatcg atcgttttga
    30061 tgctggattc aaagccggtg ttgatgcagg tgcgaaagaa ttaggaaaag aaatcaaagt
    30121 attgaatcaa tatgctggtg acttctctgc tccagataaa ggacgttcaa ttgctcaagg
    30181 tatgtatgca caaaacgcag atatcatctt ccatgcttca ggcggtacag gtaacggtgt
    30241 attccaagaa gcaaaatcat tgaacgaatc aggcgacaaa aaagtttggg ttatcggtgt
    30301 tgaccgcgac caatcagacg aaggcgaata cacattaaac ggcgaaaaga aaaacttcac
    30361 attgacatct acactgaaag cagttggaac agtagtagaa gatttggcac aaaaatcagc
    30421 ggacggtaaa ttccctggcg gcgaacacac tgtttatgga ttgaaagaag acggtgttgg
    30481 tctaacagaa ggacaacttt ctgacgaagc gaaaaaagca gttgatgaag caaaagaaaa
    30541 aatcatttca ggtgacgtaa aagttccaga aactcctgaa gaaaactaaa accatttcta
    30601 tagccttgtt agacacatgt ttgacaaggc tatggtttga ttagaacaag aattgttcat
    30661 ttatccaagc tgccttgtga cagtttggat aagggaacaa ctccctgaag taatgtttgt
    30721 ttcaaaagaa gaacgctcac taagcgtgaa tcttagtcaa tcgttgccct agatagaaat
    30781 cgttaggatg tgaaaaaaag gtggttcaag aaaatattgt catagaaatg cagaatatta
    30841 ccaaagaatt tggaacattc aaggcaaatg acaacatcaa tttacaagta aaggcaggag
    30901 aaattcatgc gttgcttgga gaaaacggtg ctggcaaatc tacattgatg aacgtgcttt
    30961 ccggattatt agagccgaca tcagggaaaa ttttgatgcg tgggaaagaa gtaaagatca
    31021 caagtccgac caaagccaac caattaggga ttgggatggt ccatcagcac tttatgcttg
    31081 ttgatgcctt tactgtaaca gaaaacatcg tgttgggaag cgagcctagt cgcgcaggga
    31141 tgcttgacca taaaaaagca cgaaaagaga tccaaaaagt ttctgaacag tatggattgt
    31201 cagtcaaccc agatgcttac gttcgtgata tttcagttgg aatggaacaa cgggtagaaa
    31261 ttttaaaaac actttaccga ggagcagatg tactgatttt tgatgagccg acagctgtat
    31321 tgacccctca agaaattgat gaattaatcg tgatcatgaa ggaattagtc aaagaaggca
    31381 agtcgatcat tttgattacg cataagttag atgaaatcaa agcagtagct gaccgctgta
    31441 cagttatccg ccgtggaaaa ggaatcggta cagtcaacgt taaagacgta acctcacaac
    31501 aattagccga tatgatggtt ggaagaacgg tttcattcaa aacgatgaaa aaagaagcga
    31561 agcctcaaga agtcgttttg tctattgaaa atctagtggt gaaagaaaat cgtggattag
    31621 aagccgtgaa aaacttgaac ttagaggttc gtgctggcga agtacttggt atcgctggaa
    31681 tcgatggaaa cgggcagtcg gagttgatcc aagctttgac tggtttgcga aaggcagaaa
    31741 gcggacatat caagctaaaa ggggaagaca tcactaataa aaaaccccga aaaatcactg
    31801 aacatggtgt aggacacgtg ccagaagacc gtcataaata cgggttagtc ctagatatga
    31861 cattatctga aaatatcgca ctacaaacct atcatcaaaa accttacagt aaaaacggca
    31921 tgctgaatta ttcagtaatg aatgaacatg ccagagaatt gatcgaagaa tatgatgttc
    31981 gaacaacgaa tgaacttgtt cccgcaaaag ctttatcagg cggaaatcag caaaaagcaa
    32041 ttatcgctcg tgaagtcgac cgagatcctg atctgttgat tgttgcaaat ccaactcgtg
    32101 gactggatgt aggtgcgatc gaatttattc ataaacgtct gatcgaacaa agggacaaat
    32161 acaaagcagt gttactgatc agtttcgaat tagaagaaat tttaaatgtt tcggatcgta
    32221 ttgctgttat ccatgaagga gaaattgtcg gtatcgttga tccgaaagaa acatctgaaa
    32281 atgaattagg tctattgatg gcgggctata ccttagaaga agcacgaaaa gagttagaaa
    32341 agggggctgg cgcacatgaa tgatcgcagc gaaaaattcc gtaatttatt agtgcctgta
    32401 ctttcagttg ttttaggatt gatcttaggc gcgatattga tggctgcttt tggttttcag
    32461 cccatccaag gttattcttc tatgctagat gcagcccttg gttcgcaaag agggataggt
    32521 gaaacgttaa ggcaagcaac gccgttgatt tttacagcat taggtttttc tgtagctaac
    32581 tcagctgggt ttttcaatat tgggctttct ggacaagcac tttgcgggtg gatcgtcagt
    32641 gtctgggtgg cacttgcgtt tccagatctg ccaaaagttg ttttgcttcc gttatgcgtg
    32701 atcttaggtg ccttagctgg tgcgatggca gcggctgttc caggtttact tcgagcattt
    32761 tttggaacaa gtgaagtgat cgtaacaatc atgatgaact atatcttatt atacgtaagc
    32821 acatttgctt tacatgaagt gatgcctgaa agctatcgct caagtttgga ctcttccaat
    32881 atgatcagtc aaaatgcctc actgcggtta ccatggttaa cacaagcttt tggcggctct
    32941 cgcgtaaact caggattatt tttagcaatc atcgcgctta ttttcgtttg gctgctgatg
    33001 aagaaaacga ctttaggatt tgaaatccgt tctgttggat tgaatccttt tgcttctgag
    33061 tatgcaggaa tgagtagtaa acgtacgatc gttttgtcga tggtcatttc tggcgctttg
    33121 gcagggcttg gcggtgtggt tgaaggatta ggaacgtatc aaaacttctt tgttcaaacg
    33181 acttcgctat ctattggatt tgacggaatg gctgtttcct tgcttggttc tggatcagca
    33241 atcggtattc ttttatcagc gatcttgttc agtattttga agatcggtgg acttggcatg
    33301 cagacaggag caggtgttcc gtttgagatc gtcaatgtat cgattgcttt gattatcttc
    33361 tttgtcggga tcaacttcct gattcgtttc gtcatggcta aagtcttcca agggaaaaaa
    33421 caagaagaga tcgttgcgac aatagaatcg aaaccaacga atcaaaacca tgaagggggc
    33481 gaactataat gtcgactatc gaattaattg ccttgatcct tacttctaca ctggtttatt
    33541 caacaccgct aattctaacg tcactaggcg gaactttttc tgaacgaagc gggatcgtaa
    33601 acgtagggct agagggaatc atggtcatgg gtgcttttag ttccatcgtt ttcaacttga
    33661 cgatgagtcc atcacttggt tctatgacac catggatcgc tattttagtg ggtggcgtcg
    33721 taggtatcct attttctctg attcacgcag tagcaacagt cagtttgcgt gctgaccata
    33781 ttatcagcgg gacagtaatt aatctgatgg cacctgcttt aggtgttttc ttgatcaaag
    33841 catggtatgg taaaggtcaa actgataaca tcacgcagaa tctggggtat ttttctttcc
    33901 caggattagc agatattccg gtcattggac caattttctt taaaaataca ttcttacctg
    33961 cttattgtgc tattttgatt gctgtcctag cttgggtgat catcttcaag acgaaattcg
    34021 gcttgcgcct ccgttcagtt ggtgaaaatc ctcaagctgc cgacacatta ggtatcaatg
    34081 tatatgctat gcgttattca ggcgtactga tttctggctt gttaggcggt atgggcggtg
    34141 ctgtttttgc tcagtcgatt tccggtaact tttcagctgg tacgatcgtt ggacaagggt
    34201 tcatctccat ggctgctatg atctttggta agtggaatcc tttaggggca atgggtgcct
    34261 cattgttctt tggttttgct caaagcttga gtatcattgg cgcacaatta ccagttattt
    34321 cttcgattcc gccagtattg ctgcaaattg ctccatattt gctgacaatc attgtgctgg
    34381 ttgttttctt agggaaagct tctggtccaa aagcaaacgg gaagaactat atcaaatcaa
    34441 aataatcttt tagcgaataa aaagggtggg ttacgggaag tgtttttcgt ctcgcccttt
    34501 ttgttttaaa ggcaataaaa ataaaaagaa agaaggaata atcatgttta aacgcgtaca
    34561 tctgatcgtc atggattcag taggaattgg agaagcacca gatgccgata agttcggtga
    34621 tgttggttct gatacattag gacacattgc gaaagaagca ggattgaacg ttccacattt
    34681 agagcagctt ggtttaggaa cgatccgtcc tcttgaaggt gtggaatcag tgaaagatca
    34741 tcaaggctat gcgacgaaat tagaagaagt ttctgtaggg aaagatacga tgacaggcca
    34801 ctgggaaatc atgggattga atatcaaaac tccatttcgt gtgtttccta atggattccc
    34861 tgatgatctg atcaaacaaa tcgaagaatt ttctggccgt aaagtcgtat gtaacaaacc
    34921 ttatagcgga acagctgtta ttgacgatta cggtgaacac caaatgaaaa caggcgattt
    34981 aattgtatat acttctgccg atcctgtcct tcagatcgcg gcacatgaag aaatcattcc
    35041 tttagaagaa ttatatcgga tttgccaatt cgttcgtgat ataacaaaag atgatccgta
    35101 tatgattggc cgaatcattg cacgtccgta cgtgggtgaa ccaggagact tcacacgaac
    35161 aagcaatcgc catgattatg cgttgaatcc attcggacac acagtccttg attctttgaa
    35221 agaagcagga aaggacgtga ttgctgttgg aaagatcaac gatattttca acggacaagg
    35281 aatcactgaa tctgtccgca cgaaaagcaa tatggatggc gttgaccagc tgctgaaagt
    35341 tatgaaaaac gatttttcag gaatcagctt taccaattta gtagattttg atgcattata
    35401 cggtcatcgt cgggatgtag tcggctatgc gcatgcgatt gaagaatttg acttacgaat
    35461 tccagaattg cttgaagcga tggaagaaga cgacttgcta atgattacag ccgatcacgg
    35521 aaacgatccg acgtttactg gtaccgatca tacgcgagaa tatgtaccgt tattggttta
    35581 cagtaaaaaa atgaaagaac aaggggcatt gcctcaagga tattattcag atatttcagc
    35641 aacgattgct gagaacttta gtgtttcttc gactgaaaat ggcgaaagct tcttgaaatt
    35701 attaaaataa tctttttaaa taggagaaag caaatgacca agttaagcga gatgttacaa
    35761 gaaacgaccc agtttatcct tgataaagga gtaaaagaaa ttgattttgg tttgattcta
    35821 ggttcaggat taggtgaatt agcagatgaa atcgaagatg ctgtaatgat ttcttatgat
    35881 gagatcccgt tctttcctgt ttcgacagta gttgggcatg caggaaagct agtttatggg
    35941 tctctggcag gcaagaaagt gttagccatg caagggagat tccactacta tgaaggtcat
    36001 tccatgcaaa cagtgaccta tccagtacgt gtgatggccg gactaggggc ccattcagtc
    36061 attgtgacta atgcatgtgg tggtgtcaac gaatcatttg taccaggaga tttgatgctg
    36121 attaccgatc atatcaattt tactggtgaa aatcctttga tcggtccgaa tgaagatgca
    36181 atgggcccta gatttccaga tatgagtcaa gcttatacgc ctgactatca agcaacagca
    36241 aaagcagcag cagaaaaact aggtttggat cttaaagaag gcgtatatat gggctattct
    36301 ggaccaactt atgaaacgcc tgcggagatc cgaatgtctc ggacaatagg agccgatgca
    36361 gtagggatgt cgactgttcc agaagtcatc gtagcagctc acagcggatt aaaagtgtta
    36421 ggaatctctt gtattaccaa cttagcagca ggtatgcaag cgaatctgag ccatgaagaa
    36481 gttgtagaaa caactcagag agtcaaacaa tcttttaaag cattggttaa agaaacattg
    36541 acattattgt aataggagga acaataaaat gagtgtgcat attgaagcga aagaaggcga
    36601 gatcgccgat aagattttac ttccgggaga tcctttgcga gctaaatata ttgcggagac
    36661 ttttttggaa gatccagttt gttacaatca ggtacgcggt atgttaggct ttaccggcaa
    36721 atataaagga gaacgtattt ctgttcaagg aacaggaatg ggaatgccat cagccaccat
    36781 ctatgcccat gaattgatcc agtcgtatgg tgtaaagaaa ttgattcgag taggtacatg
    36841 cggtgcattg tcaaaagacg tccatgttcg tgatttagta ctagcacaag gtgcagcgac
    36901 cagttcttcg atgatcgaaa aaaatttcca agcctttcat tttcctccaa tcagtgattt
    36961 caatctatta ctgaaagcat atgagatagc taaagaaaag ggctatacgg ttcatgtagg
    37021 aaatgttctt tcggaagatt ctttctacaa agatgatttg acagagacgt tccaattagc
    37081 tgaacttggc gttttaggag tggaaatgga agcagcggct ctttattact taggcgcgaa
    37141 ataccatgtg cagacattaa gtttgatgac cgttagcgac catttgatca ccggtgaaga
    37201 aacaacagct gcagaacgtc aatctacgtt caatgaaatg atcgaagtag gattggaaac
    37261 agcgatagca gaataagaag gaaatgaaaa agctgccctg cattaagtaa taagaacatc
    37321 caagcaaaaa tagctcctca tgtttttgac ttggctggac ttattattgc aaatgtaagc
    37381 ttttgaacac cgtttaccaa gaggttgtga atctgccgtt taactctgag tcacaaggaa
    37441 caatttcaaa aaacagcttt tcatgtttga tgaatattgt gtcttgtgac cgagaagttg
    37501 gcaggagaaa accgtttata cgaaaaaagg gactgtgaca aatcgtgtca cagtcccttt
    37561 tttatagtgt tttttatagg tagtattttt tagcaactgt taagtcatca tttaattcat
    37621 aaacaagtgg ttgacctgtt ggaatttcaa gatccatgat gtcttcgtca gagatacctt
    37681 cgatatgttt agctagggcg cgtaatgagt taccatgtgc agctactaaa acagttttgt
    37741 tgtctagtaa tgctggagca atttcatctt gccagaatgg caaagcgcgt tctaatgtca
    37801 ctttcaagtt ttctccacca ggaatatcgc gtttgtctaa cattgcgtaa cgacggtcat
    37861 ttgctgcaga accttcgtca gtttcttcca ttaatggagg aagagtatcg taagaacgac
    37921 gccagatgtg cacttgttcg tcgccgtatt tttcagctgt ttcttttttg ttcaaacctt
    37981 gtaatttacc atagtgacgt tcgtttaagc gccatgattt gatttgtggt acccaaagtt
    38041 gatctgaata ttctaagatc aagttacatg ttttgattgc acgagttaaa acagaagtgt
    38101 aagcaacatc aaattcgatt ccagcttctt tgattttgcg tccgccttct tttgcttctt
    38161 cgattccttc tggtgctaga tttacatcag cccaaccagt aaattggttc aatgcattcc
    38221 attcactaag accgtgacga gaaaaaacta attttggcat gtttagctct ccttttaaga
    38281 taggtttata tttctgaaat tttcagttct gttttattgt acaatttttt ggaagagatt
    38341 tccatcgaaa aattagataa gattctatga gaaaacaagt agagaatata aaaaaacgaa
    38401 cagtgcccac gcaactgttc gtttttaggt attagatgca taattgctcg atttcttttt
    38461 caggtaaatt gatatattta taacatgttc caactgaaac accgcatttt gaagaaataa
    38521 attggatcgt ttcttttttt tcataataga gacgtcggat ttttttgatc gtttttgcat
    38581 cgatttttgg tcttccgccg atttttcctt ttttacgagc ttcatctaat ccaactaatg
    38641 tacgttcttt gatcaatgcg cactccatgt ttgctaggtt ttccattaat tggaaataga
    38701 tttttcccat ttcgcgcgta tcgattcctt catcaagact aatcaagtgc agcccgcgat
    38761 ctttgaataa ttgcgtgagt tctgttaatt gccgcgtaga tttgcctaat cgatgaagtt
    38821 ggcaaatgac tagtgcatcg ccttgttgca tgtcagcgat cactgactcc aaatgaacag
    38881 cagtagaatc attttgttcc acttcatatg tctcatggaa aatttcttga caaccaaagt
    38941 cagataattg atcaacctga gttgttaaat caacgtcatt tatggttgtg cgtgcataac
    39001 caataatttt catggtgatt cctttctagc agtgattttc taactggata taacagtaaa
    39061 aatgtagcat aaagtacgtc aaaaagatag ttttttcgca aattttccgc tgtctttttt
    39121 tattggatga tcaagcagtt tttctttatt tacgacggat tgcgtcaaga atctcaacgc
    39181 ctttttctga gccgacaact actttagaaa ccatgtttag gaaaagccca tgttctacta
    39241 ccccaacaag ttcatctaaa taggtagcta agctttctgg ctgttcgatg acttctagat
    39301 gcagatcaat gatgtaatga ccaccatctg tggttaagac ttctccgttt tcgtctgtac
    39361 gcaagacggg ttggaatctt ttttcttcaa aaatatgaag tagctgttgg gaaccataag
    39421 ggatcacttc aactgggagt gggaacttcc caagtttatc gactaattta gaagaatcaa
    39481 cgatccaaat cgtttctttt gagtacgtag cgactatttt ctcgaaaagc aaagcagcgc
    39541 cgccgccttt gatcccttga aaatcagcac tgatttcatc agcaccgtct attgtcaaat
    39601 cgactacggg gacttcatca atactcttta acggtattcc taatttttcc gcttgttctt
    39661 ttgtcgcatt tgaagtagta attcctgtga tacgtaaacc ttcttcacgc atacggcgac
    39721 caatctcttc taccatataa taggcggttg aaccggtccc gagacctacg atcatcccgt
    39781 cctttacgaa tttcgcggct tcaatgccag ctaattgttt taaattcatc tttgcgtcca
    39841 ccttttttta gtttgagcat cgtgctttcc tcactatttt agcgtgattt acggtaaaaa
    39901 gataccattc ttttttattc caataaagct ttttcaaaaa aaattgcatc tcttgttgac
    39961 atgttttttc attcgtgata ttctaacaaa ggtgctttat ctacaggttt ctgtgaagaa
    40021 acgtgctgac tgtttatcaa agcatatttg ataaggatgc agacattgca tttatttttt
    40081 atcacaaaaa aagaaccacc tggatgtgtg gaactagacg caaatcgtgg gaaggaggaa
    40141 acaaggaatg cctacaatta accaattagt acgtaaacct cgtaaatcaa aggtggaaaa
    40201 atctaattca cctgctttga acaaaggata taacagcttt aagaaaactc aaactaacgt
    40261 aaactcacct caaaaacgtg gggtagcaac tcgtgtgggt acaatgacac ctaaaaaacc
    40321 gaactcagct ttacgtaaat atgcacgtgt tcgtttgtca aacttaattg aagttactgc
    40381 ttatatccca gggattggtc ataacttaca agaacacagt gttgtattat tacgcggtgg
    40441 tcgtgtaaaa gacttaccag gggtacgtta ccatatcgtt cgcggtgctt tagatactgc
    40501 tggtgttaac gaccgtaaac aaagccgttc taaatacgga actaaaaaac ctaaagctta
    40561 attttaatta ttaaacaaat gaaacttgct tctactatat agaagtaatt actatgaaca
    40621 atatttcgga aggaggagtt acggatgcca cgtaaaggtc ctgttgcaaa acgtgatgtt
    40681 ttaccagatc ctatttataa ctcaaaatta gtaactcgct tgatcaaccg tgtaatggtt
    40741 gacggaaaac gcgggattgc agctaacatt atctataatg cctttgactt gatcaaagaa
    40801 tctacaggta acgatccatt agaagtgttt gaacaagcaa tgaaaaacgt tcaacctgtt
    40861 cttgaagtta aagcacgtcg tgttgggggt tcaaactacc aagtaccagt tgaagttcgc
    40921 cctgaacgcc gcgttactct aggtcttcgt tgggtagtaa actacgctcg cttgcgcggt
    40981 gaacacacaa tggaagaacg cttagcaaaa gaaatcatgg atgctgcaaa caacacaggt
    41041 gcttctgtta aaaaacgtga agacactcat aaaatggctg atgccaaccg tgcgtttgct
    41101 cactatcgct ggtaagattc tctctgtttg gtcggctttt ataagtcgac ctcagatagt
    41161 gtccttattt ttagcaacta gatagtaaag agaggagaaa caaggagaat gaccagagaa
    41221 ttttctttac aaaacactcg taatatcggt atcatggccc acatcgatgc tggtaaaacg
    41281 actgctactg aacgtatctt gtactatact ggtaaaatcc ataaaattgg tgaaactcat
    41341 gaaggagctt cacaaatgga ctggatggaa caagaacaag aacgtggtat tacgatcact
    41401 tctgctgcta ctacagcaca atggaagggt caccgaatca acatcatcga cacaccagga
    41461 cacgtagact tcacagttga agttgaacgt tcattgcgtg tacttgatgg tgctgtaact
    41521 gttcttgatg cacaatcagg tgttgaacct caaactgaaa cagtttggcg tcaagcaaca
    41581 acttatggtg tacctcgtat cgtatttgca aacaaaatgg acaagatcgg tgctgacttc
    41641 ttatattcag tatcaacttt acatgaccgt ttgcaagcaa acgctcaccc aatccaatta
    41701 ccaattggtg cggaagacga cttcacagga attatcgacc ttgtaacaat gaaggctgaa
    41761 atgtatacaa acgatcttgg aacagagatc gaagaaacag aaattcctga agaataccgc
    41821 gaattagctg aagaatggcg cgaaaaattg gttgaagctg tagctgaaac tgatgaagaa
    41881 cttactttga aatacttaga aggtgaagaa atcactgaag ctgagttaaa agaaggtatc
    41941 cgccgcgcaa cagttaacgt agaattctac ccagtactat gtggttcagc atttaagaac
    42001 aaaggggttc aattactatt ggacgctgtt cttgattact tgccatcacc gttagatatc
    42061 ccagctatca aagggattga ccctaaaact gatgaagaag tagaacgtcc tgcagacgac
    42121 agcgctcctt tctcagcttt agcatttaaa gttatgactg acccattcgt aggtcgttta
    42181 actttcttcc gtgtgtactc tggtgtttta caatcaggtt catacgtaca aaacgcaaca
    42241 aaaggcaaac gtgaacgtgt tggtcgtatc ctacagatgc atgccaactc tcgttcagaa
    42301 atctctgaag tttacgctgg agatatcgct gctgctgttg gtttgaaaga cacaactact
    42361 ggggacacac tatgtgatga aaagagtcta gttattcttg aatccatgga attcccagaa
    42421 ccagttatcc aagtcgctat tgaacctaaa tcaaaagctg accaagataa aatgggtgtg
    42481 gctttacaaa aactatctga agaagatcct actttccgtg ctgaaacaaa cgtggaaaca
    42541 ggcgaaacaa tcatcgctgg tatgggtgaa cttcacttag atatcatcgt tgaccgtatg
    42601 cgtcgtgaat tcaaagttga agctaacgta ggtgcgcctc aagtatctta ccgtgaaact
    42661 ttccgtgctg gcactcaagc cgaaggtaag tttgtacgtc agtctggtgg taaaggtcaa
    42721 tacggtcacg tatggattga atttacacca aatgaagaag gtgctggttt cgaatttgaa
    42781 aatgcgatcg tcggtggggt tgttcctcgt gaatacattc cagctgttga aactggtttg
    42841 aaagatgcta tggaaaatgg tgtcttagct ggctatccat tagtagatat caaagctaaa
    42901 ctttacgatg gttcatacca tgatgtcgat tcaaatgaaa ctgccttccg tgttgctgct
    42961 tctatggcat tacgtgcagc agctaagaaa gctaatccag taatccttga accaatcatg
    43021 gctgtagaag ttgttatccc tgaagattat ttaggtgatg ttatgggtca cgtaactgct
    43081 cgtcgtggac gtgttgaagg tatggaagct cgcgctggcg gccaacaagt tgttcgtgcg
    43141 atggttccac ttgctgaaat gtttggttat gctacaactc ttcgttctgc tactcaaggt
    43201 cgtggtacat ttacaatgac atttgaccac tacgaagatg taccaaaatc agtacaagaa
    43261 gaaatcatta agaaaaacgg cggaaaagct gaataatttc ttaaaaccgg atagtcctgc
    43321 aaagagatta tccaaagaga ctatattttt aatatagttt cgtgtaaaat aattatcgat
    43381 gatatgggca tgtggtgtac cttgtgtaca tcatatacct atcagacaaa taaaaaatat
    43441 attgtatttt taggaggaca ttttaaaatg gcaaaagaaa aatttgaccg ttctaaacca
    43501 cacgttaaca ttggtactat cggacacgtt gaccatggta aaactacatt aacagctgca
    43561 atcacaactg tactatctaa gaaaaacggc ggccaagcta tggcttacga tcaaatcgat
    43621 ggtgctccag aagaacgcga acgcggaatc acaatctcaa cagctcacgt tgagtatgaa
    43681 acagatacac gccactacgc tcacgttgac tgcccaggac acgcggatta cgttaaaaac
    43741 atgatcactg gtgctgctca aatggacgga gctatcttgg tagtttctgc tgctgacggc
    43801 ccaatgcctc aaactcgtga acacatccta ttgtctcgtc aagttggtgt tccttacatc
    43861 gttgtattct tgaacaaagt agacatggtt gatgacgaag aattactaga attagttgaa
    43921 atggaagttc gtgacctatt aacagaatac gaattccctg gtgacgatgt tcctgtagtt
    43981 gctggatcag ctttgaaagc tctagaaggc gacgcttcat acgaagaaaa aattcttgaa
    44041 ttaatggctg cagttgacga atacatccca actccagaac gtgacaacga caaaccattc
    44101 atgatgccag ttgaagacgt gttctcaatt actggacgtg gtactgttgc tacaggtcgt
    44161 gttgaacgtg gacaagttcg cgttggtgac gaagttgaag ttgttggtat tgctgaagaa
    44221 acttcaaaaa caacagttac tggtgttgaa atgttccgta aattgttaga ctacgctgaa
    44281 gctggagaca acattggtgc tttactacgt ggtgttgcac gtgaagacat ccaacgtgga
    44341 caagttttag ctaaaccagg tacaatcaca cctcatacaa aattctctgc agaagtatac
    44401 gtgttgacaa aagaagaagg tggacgtcat actccattct tcactaacta ccgtccacaa
    44461 ttctacttcc gtacaactga cgtaacaggt gttgttgaat taccagaagg aactgaaatg
    44521 gttatgcctg gcgacaacgt aacaatggaa gttgaattaa tccacccaat cgctatcgaa
    44581 aacggtacta aattctcaat ccgtgaaggc ggacgtacag ttggtgccgg tgtcgttaca
    44641 gaaatcaaag cttaattcac ttaagtttta aagtaaagca acatttattc ggacgtaatc
    44701 gctcggaacg aaggtctcag gatctttgtt ccgagctttt ttatttattg caggatcagg
    44761 aaaaaagaca ggagtattat aatagaagga acgcgaaata aaagagtcag tattttctaa
    44821 atgaaacttt atgaaaacaa aatgtttttt acaagaaaaa gccttgcttt gtgagtttat
    44881 atatattata atgacaaagg tgcgtttact ttagtgaatt gcacagacgt aatcagaatg
    44941 atttcgtcgg ctaagaaacg agaggttgcg acacgcccgg acgctttgcc acgaacgagc
    45001 gtgacggaaa ttttcgtgga gctatgtcta cttttcaaaa taggcgaagg agggaacaag
    45061 atggcaaaac aaaaaattcg tatccgttta aaagcgtatg aacaccgtat tttagatcaa
    45121 tcagcggata aaatcgtgga aactgcaaaa agaactggag ctagcgtgtc tggtccaatt
    45181 ccattaccaa ctgaacgcag tctttacaca atcatccgtg cgactcataa atacaaagat
    45241 tcacgcgaac aattcgaaat gcgtacacac aaacgtctta tcgacattgt gaacccaaca
    45301 ccaaaaactg ttgatgcttt gatgaagcta gacttaccat ctggtgtaaa catcgaaatc
    45361 aaattataaa actaaaacaa aaataatgga ggtgtactca tgaccaaagg aatcttaggg
    45421 aaaaaagtgg gaatgacaca aatcttcact gaatctggtg aattaattcc agtaactgtt
    45481 gtagaagcta ctccaaacgt agtactacaa gtgaaaacaa tggaaacgga tggctacgaa
    45541 gctattcaag ttggttacca agatatgcgt gaagttttat caaacaaacc tgcgaaaggt
    45601 catgttgcaa aagcaaacac ggctcctaag cgcttcattc gtgaattcaa aaatgttgag
    45661 ctaggagaat atgaagtagg aaaagaaatt acagtagatg ttttccaagc aggagacatt
    45721 attgatgtaa caggtactac gaaaggtaaa ggattccaag gggttatcaa acgccacgga
    45781 caaagccgcg gaccaatggc tcacggttct cgttaccatc gtcgtcctgg gtcaatgggt
    45841 ccagttgcac ctaaccgtgt atttaaaaac aaacgtcttg ctggccgtat gggtggcaac
    45901 cgcgtaacta tccaaaactt ggaagttgta cgtgtcgatg cagaaagaaa tgttatcttg
    45961 atcaaaggta atgttcctgg agctaaaaaa tctttaatca ctatcaaatc agctgtgaaa
    46021 gctaaataat tgtaggaaga aaggaggaac taaggaatgc cgaatgtagc attattcaaa
    46081 caagatggaa gccaaaacgg tgaaatcaca cttaacgaag aaatcttcgg aattgaacct
    46141 aatgaaagtg ttgtttacga tgcaatcgta atgcaacgtg cgtcattaag acaaggaaca
    46201 cacgcggtta aaaaccgtag cgctgttcgt ggcggtggac gtaaaccatg gcgtcaaaaa
    46261 ggaacaggtc gtgcacgtca aggatctatc cgctcaccac aatggcgtgg aggtggcgta
    46321 gtctttggac caacaccacg ttcttacagc tacaaacttc ctaaaaaagt tcgtcgttta
    46381 gcaatgaaat ctgtattgtc agaaaaagtt gctgaaaaca acttggtagc aatcgaaggc
    46441 ttgaacttcg acgcaccaaa aacaaaagaa ttcaaacaag ttcttgctaa cttatctatc
    46501 gactctaaag tattggttgt tctagaaaaa ggtaacgact ttgctgcttt atcagcacgt
    46561 aacttaccaa acgtttctgt agtaacttct gataatgtga gtgtgctaga cgttgtatca
    46621 aatacaaaag tgttagcaac acaaactgct cttactcaaa ttgaggaggt gcttgcataa
    46681 tgaacttact agacgtaatc aaacgcccag tgatcactga aaaatcaatg cttgccatgg
    46741 atgacaagaa atacactttt gaagtggaca ctcgcgcaaa caaaacttta gtaaaacaag
    46801 cggtagaagc tgcttttgac gttaaagtca aaaacgtgaa catcgtgaac gtacgtgcga
    46861 aattcaaacg tatgggcaaa tatgcaggat atacaaaaaa acgtcgcaaa gcgatcgtta
    46921 cattgactga agattcaaaa gaaatccaat tattcgaagc tgctgaataa gcactaatag
    46981 ataactcatt aaattaggag ggaaaaacgt ggcgattaaa aagtacaaac ctacctcaaa
    47041 tggtcgtcgt aacatgacag gttctgattt tgctgaaatc acaacatcaa cacctgaaaa
    47101 atcattgtta caaccattga aaaaccatgc tggacgtaac aacaacggcc gcatcactgt
    47161 acgtcatcaa ggtggcggac acaaacgcca atatcgcgtg atcgacttca aacgtaacaa
    47221 agataacgtt gttgcaactg ttcaaacgat cgaatatgat ccaaaccgtt cagctaacat
    47281 cgcgttagta cactatgaag atggagtgaa agcttacatc ttagcaccta aaggactaga
    47341 agtgggtatg aaacttgttt caggtccaga agcagatatc aaagtcggaa atgctcttcc
    47401 attagaaaac attccagttg gtactgttat ccacaacatt gaaatgaaac ctggtaaagg
    47461 tggacaattg atccgttctg ctggtacaag tgctcaagta cttggtaaag aaggcaaata
    47521 cgtattgatc cgcttgaact ctggtgaagt tcgtatgatc ttagctgcat gccgtgctac
    47581 aatcggttct gtaggtaacg aacaacacga attaatcaac atcggtaaag ctggacgctc
    47641 tcgttggatg cgtaaacgcc caactgtacg tggtagcgta atgaacccta atgatcaccc
    47701 acacggtggt ggtgaaggta aagctcctat cggacgtaaa gcaccagttt ctccttgggg
    47761 tcaaccagct cttggttaca aaactcgtaa caaaaaagct aaatcagaca aacttattgt
    47821 tcgtcgtcgc aaaaataaat aattactttg accatgtgtc gcacattttg agaggaggtt
    47881 caccatgggt cgtagtttaa agaaaggacc ttttgtcgat gagcatttaa tgaaaaaagt
    47941 tgaagctcaa gcaggggttg aaaagaaaaa agttatcaaa acttggtcac gccgttcaac
    48001 agttttccca agttttgttg gatataccat cgcagtttat gatggacgta aacatgtacc
    48061 agtttacatc caagaagaca tggtaggaca caaattaggt gaatttgcac ctacaagaac
    48121 ttatcgtgga catgctgctg acgataagaa aacgaaacgc taatttgagg ggaggataaa
    48181 tccaaatggc agaacaaatc acatcagcta aagcaactgc aaaaacagtt cgcacttcac
    48241 ctcgaaaagc ccgtttagta attgacctta tcagaggtaa aagtgtcgca gatgcaattt
    48301 caatcttgaa attcacacca aacaaatctg ctggaatcat tgaaaaagta ttgatgtcag
    48361 cagttgcgaa tgcagaaaat aactttgact tagatgttga aaacttggta gtaagcgaag
    48421 cttttgttaa cgaaggacct acaatgaaac gtttccgtcc tcgtgcaaaa ggatcagctt
    48481 ctccaatcaa caaacgtaca agtcatatca cggtagtcgt atcagaaaaa taaggaggga
    48541 taatcagtgg gtcaaaaagt acatccaatt ggaatgcgtg taggcatcat ccgcgactgg
    48601 gatgctaaat ggtatgctga aaaagagtat gctgagttct tacacgaaga tttaagaatc
    48661 cgtaaattta tttcaactaa acttgctgat gctgctgtat caacagttga aatcgaacgc
    48721 gcaacaggtc gcgtaaacat ttcaattcac acagctaaac caggtatggt tattggtaaa
    48781 ggcggttctg aagtcgaaag cctaagaaaa gaattaaaca aattaactgg taaaagaatc
    48841 catatcaata tcgtggaaat caaaaaacca gatctagatg caaaattagt aggtgaagga
    48901 atcgcacgtc aattagaaaa ccgtgttgct ttccgtcgcg ctcaaaaaca agccatccaa
    48961 cgctcaatgc gtgctggcgc tcaagggatc aaaactcaag tatctggtcg tctaaacgga
    49021 gcagatatcg ctcgttcaga aggatactca gaaggaactg ttcctcttca tactttacgt
    49081 gcggacattg attacgcatg ggaagaagca gatacaacat acggaaaact aggagttaaa
    49141 gtgtggatct atcgtggaga aattcttcca actaaaaaaa acactgagaa aggagggaaa
    49201 taatcatgtt agtacctaaa cgtgtaaaac accgtcgtga attccgcgga aaaatgcgcg
    49261 gggaagctaa aggcggaaaa gaagtagcat tcggtgaata cggtttgcaa gctgttgatt
    49321 cacattggat cacaaaccgc caaatcgaag ctgctcgtat cgcaatgact cgttacatga
    49381 aacgtggtgg gaaagtatgg attaaaattt tccctcacaa atcttatact gccaaagcaa
    49441 ttggggtacg tatgggttct ggtaaagggg cacctgaagg atgggttgca ccagtaaaac
    49501 gtggtaaaat catgtttgaa atcgcaggcg ttcctgaaga agtagctcgt gaagcgttac
    49561 gtctagcttc tcacaaatta ccaatgaaaa ctaagatcgt aaaacgtgag gaaatgggtg
    49621 gtgaatcgaa tgaaggttaa agaaatcaga gaattaacca ctgccgaaat gcttgatcaa
    49681 gaaaaacaat taaaagaaga attgtttaat cttagattcc aattagcaac aggtcaatta
    49741 gaaaacactg cacgtattaa agaagtacgt aaatcgattg cacgcatcaa aactgtgttg
    49801 cgtgaacaag ctaagtaatt atggaaggag gccattaagc gtatgactga aggaagaaat
    49861 caacgcaaag tttatcaagg tcgcgtggtt tcagacaaaa tggataaaac aattaccgtt
    49921 gctgtcgaaa caaagaaaaa ccaccctatc tatggcaaac gcatgaacta ttctaagaaa
    49981 tacaaagtgc atgatgaaaa caacacagca aaagttggcg acatcgtgag aattatggaa
    50041 actcgtccat tatcagctac aaaacgtttc cgtttgttgg aagtagtcga agaagcagtt
    50101 attatctaat aacacgagat tttcctatat agattgaatg caaaatctga aaggaggata
    50161 cacaacgtga tccaagcaga aagtcgttta aaagtcgctg ataactcagg cgcacgtgaa
    50221 attttgacga tcaaagtcct tggtggatct ggtcgtaaaa ctgctaacat cggtgatgtg
    50281 atcgttgcta cggttaaaca agcaacgcca ggtggggttg tcaaaaaagg tgacgtcgtt
    50341 aaagccgtta tcgttcgaac aaaatcagga gctcgtcgta cagacggttc atatattaaa
    50401 tttgatgaaa atgctgcagt gattattcgt gatgataaaa gccctcgtgg aacacgtatc
    50461 tttgggccag ttgcacgtga actacgtgaa aacaacttca tgaagatcgt ttcactagca
    50521 ccagaagttt tataatcttg agacacttat caaaggaggt gcgaaacagt tatgtttgtt
    50581 aaaaaaggcg ataaagttaa agttatcact ggaaaagaca aaaacaaaga aggcgttgta
    50641 ctagcagcgt tcccgaaaaa agataaagtt attgtcgaag gtgtaaatgt cgttaaaaaa
    50701 caccaaaaac cttctcaagc tgctcctcaa ggtggaattg ttgagatgga agcgccaatt
    50761 catgtttcta acgtgatggt ggttgactct actggtgtag ctggccgagt tggctacaaa
    50821 gaagtagacg gcaaaaaagt ccgtgtttct aaaaaaaccg gtgaagtttt agataaataa
    50881 aagtttaagg aaggagggtc ttactgaatg aaccgcctaa aagaaaaata tcttaaagaa
    50941 gtaactccat cattgatgga aaaatttaac tatagctctg ttatgcaaac acctaaagtt
    51001 gaaaaaatcg tcatcaacat gggtgttggt gatgcggtat caaacgctaa aaacttagat
    51061 aaagcagttg aagaattaac tttgatcact ggtcaaaaac caatgattac aaaagctaaa
    51121 aaatcaatcg ctggattccg tttacgtgaa ggaatgccaa ttggtgcaaa agttacattg
    51181 cgcggagaaa gaatgtacga attcttagac aaattggttt ctgtttcact accacgtgta
    51241 cgtgacttcc atggtgtaag taaaaaagcc tttgacggtc gtggaaacta tactttaggt
    51301 attaaagaac aattgatctt cccagaagta gattatgatt tagtagataa agtacgtggt
    51361 atggacatcg ttattgtaac aacagcaaac tctgatgaag aatctcgtga gttgttgaca
    51421 caattaggca tgccattcca aaaataatta aaggaggcga actacgtggc taaaaaatca
    51481 atgattgcta aaaacaaacg tcctgcaaaa ttttcaacac aagcttatac tcgttgcgaa
    51541 cgttgcggac gtccacattc agtttatcgt aagttccatc tttgccgtat ttgcttccgc
    51601 gaacttgcct ataaaggtca aattcccggc gtgaagaaag ctagctggta aaaagtaaat
    51661 tcgcataaga aggaggtaaa agatcaatgg tcatgacaga tccaattgca gattttctaa
    51721 ctcgtatccg taacgcaaac atggttaaac atgagtcttt agaagtacct gcatcaaaaa
    51781 ttaaacgtga catcgctgaa atcttgaaac gtgaaggttt cgttcgtgat gttgaataca
    51841 tcgaagatga caaacaaggc gtgatccgtg ttttcctaaa atttggtaaa aacggcgaac
    51901 gtgttatcac aaacttaaaa cgtatctcta aaccaggttt gcgtgcttat gtcaaagctg
    51961 acgaagtacc aaaagtattg aacggtttag gtatcgcaat catttcgact tctgaaggtg
    52021 ttgtcactga taaagaagct cgtgaaaaaa atatcggcgg cgaagttatc gcttacgtat
    52081 ggtaatcaag tagaatctac aaggaggtgt ctttaagtga gccgtatcgg aaataaagtc
    52141 gttgttatcc ctgaaggagt aacagttact caagacggaa acaacgttac agttaaagga
    52201 ccaaaaggtg aattgactcg tactttttca gcagatatca aaatgaatat cgaaggaaac
    52261 gaagtaactt tcactcgtcc aaacgacagt aaagaaatga aaacaattca tggaacaact
    52321 cgtgcgaact tcaacaacat ggttgtaggt gtcagcgaag gtttccaaaa agcgttagaa
    52381 ctaatcgggg ttgggtaccg tgcacaatta caaggatcaa aacttgtatt gaacgttggt
    52441 tactcacatc cagtagaaat cacaccacca gctggagtaa ctgttgaagt accagctaac
    52501 acacaagtaa ttgttaaagg tgcgaacaaa gaagaagttg gcgaattagc tgcaaacatt
    52561 cgtggaactc gtcctccaga accttataaa ggtaaaggta ttcgttatgt tggcgaatat
    52621 gtacgccgta aagaaggtaa aactggtaaa taatcgttta gaaaaattgc atagaagcca
    52681 gcagcaggaa aaaggcattt ggctgaagtt taccttcaaa aaaatacatt tttacttgtg
    52741 ccgcagcttc tatttttcga aacgtattta ctgcttgaaa tagtagtaca ataaaaataa
    52801 agaggtgaca attgtgatta caaaaccaga taagaacaaa acacgtcaaa agagacatcg
    52861 tcgtgtacgt aacaatatct ctggtactgc tgagcgccca cgcttgaacg tttttcgttc
    52921 taacaaaaac atctacgctc aaattattga tgacgtagcg ggtgtaacgc tagcaagtgc
    52981 ctctaccttg gataaagaaa tttcaggtgg aacaaaaaca gaagcagcac aagctgtcgg
    53041 taaactagtt gctgaacgtg cagctgaaaa aggcattaaa gtagtagtct ttgaccgtgg
    53101 tggatacctt taccatggcc gtgtgcaagc tttagctgaa gctgctcgcg aaaatggact
    53161 agaattttag aagaaggagg aataccattc atggtttata tcgatccaaa acatttggaa
    53221 ttagaagacc gcgttgttgc aattaaccgt gtaacaaaag ttgttaaagg tggacgtcgt
    53281 ctacgttttg ctgctttagt tgttgtcggt gacaaaaacg gacacgtagg gtttggtaca
    53341 ggtaaagctc aagaagttcc tgaagcaatc cgtaaagcaa ttgaagatgc gaagaaaaac
    53401 ttagttgaag tacctatggt tggttcaaca atcccacacg aagtaatcgg tgcttttagt
    53461 ggtggtagaa tcctaatgaa acctgctgta gaaggttctg gggttgctgc tggtggacca
    53521 gttcgtgccg tattagaatt agctggggta gcagatatca cttctaaatc tttaggctca
    53581 aatacaccaa tcaacgttgt tcgcgcaaca gttgaaggtt tgaaacaatt gaaacgtgca
    53641 gaagaagttg cagaacttcg cggcaaatca gttgaagaat taatcggata aggaggacaa
    53701 caataatggc tgaattaaaa gttactttaa aacgcagtat tatcggacgt cctcaaaacc
    53761 aacgcgatac agttaaagcg ttaggtctta caaagattaa cagttcagtg gttaagcctt
    53821 ctaatgaagc aattaaaggc atgatcaata ctgtttctca cctagtggac gttgaagaaa
    53881 tttaatttag aaaaatcata actagaacat taaggaggtg ccaaacctat gaaacttcat
    53941 gaattaaaac ctgctgaagg ttcacgccaa gtacgtaacc gtgttggtcg tggtacttca
    54001 tctggtaacg gaaaaactgc cggacgcgga caaaaaggac aaaaagctcg ttcaggcggc
    54061 ggtgtgcgtt taggatttga agggggacaa acaccattgt tccgtcgttt accaaaacga
    54121 ggattcacaa acgtgaaccg taaagactat gcagtcatta atcttgatgt attgaaccgc
    54181 tttgaagacg gagctgaagt aactcctgtt gctttagttg aagctggaat cgtgaaaaac
    54241 gaaaaagctg gaatcaaagt attagctaat ggggaactaa acaagaaatt aactgtgaaa
    54301 gcagcaaaat tctctaaagc agctcaagaa gcaattgaag cggctggtgg atcaatcgag
    54361 gtgatctaat gtttagacta ttaaaagatg cttttaaagt aaaagacatt agatcgaaaa
    54421 tcctatttac tgtatttgtg ttgtttgtat ttcgcctagg tgcacatatt actgtaccag
    54481 gcgtaaatgc tgcttcattg caaaaccttt cttctttgcc atttttcaac atgttgaact
    54541 tggtaagtgg tagtgcgatg caaaactttt ccattttctc aatgggggtt tcaccataca
    54601 ttactgcttc tatcgtcatt cagcttcttc aaatggacat cgtgcctcga tttgttgaat
    54661 ggtcgaaaca aggggaagtt ggtcgtaaaa aactgaatca agcgactcga tatctgacat
    54721 tagtattggc atttatccaa tctgtcggta tcactgctgg attcaatcaa tacgctcaat
    54781 taggtttcgt gaataatcct aacgtagcaa cttacgtaac gatcggtttg attctaacag
    54841 ccggtacgat gtttgttact tggctaggag aacagatcac agaaaaaggt atcggaaatg
    54901 gcgtatcaat gatcattttt gcaggtatca tttctagatt gcctgaaagt atcagagaaa
    54961 tttatgaaga ttactttata aatattgatc cttcagacat atggaaatct gctatttttg
    55021 tggccatttt actagtagcg gtcttgttga tcgtgacatt cgtaacattt ttccaacaag
    55081 ctgaacgcaa gatcccgatc caatatacga aacgcgtagc tggcgcgcct acaagcagct
    55141 atctgccgtt gaaagtaaat gcagctggag ttattccagt tatctttgct agttcgttta
    55201 ttgctacgcc aaatgctgtt ttacaagcat tgagcggtag attcggttct gaaaactggt
    55261 acaaagtgat gatggagatc tttaactata atacagcacc tggtgctacg atttatacga
    55321 ttctgatcgt tgccttcact ttcttctacg cctttgttca agtaaaccct gagaaattag
    55381 cggaaaactt gcaaaagcaa ggaagttata taccaagcgt acgccctggt aaggggactg
    55441 aagagtatgt ctcaagatta ctgatgcgtt taagtacagt tggtgcagtc ttccttggtt
    55501 tagtggcatt gcttccaatc attgctcaaa tggtttggga tcttccacaa tcaatcggat
    55561 taggcggtac aagcttactg atcgttatcg gtgtagcttt agaaactgcg aaacagttag
    55621 aaggcttaat gctgaaacgt aaatatgttg gatttattga gtaagaaagc gtgttgaggt
    55681 ttcccctcaa cacacgttct cacacattta ttaggaggga aaacaatgaa cctcatttta
    55741 atggggctgc caggcgcagg taaaggaaca caagctgaaa aaatcattga tgcttatggt
    55801 attccgcaca tctcaacagg agatatgttc cgcgcagcga tgcaaaacga aacagctctt
    55861 ggcctggaag caaagtctta tatggataaa ggtgcattag ttcctgatga agtaacaaat
    55921 ggaatcgtga aagagcgact agctgaacca gatacagaga aaggcttctt attagatggc
    55981 tttccacgta cgctagatca agcaaaagct ctggacgcaa tgctaaaaga tttgaataaa
    56041 aaaattgatg ctgtcatcga tatccatgtt ggtgaagaaa tcttggtcga acgtctagcc
    56101 ggccgattta tctgcagcaa ttgtggagca acttaccata aaattttcaa ccctacaaaa
    56161 gttgaagaca catgtgatcg ttgcggtggg catgaattct atcaaagaga agatgataaa
    56221 cctgagacgg ttaaaaatcg tctggctatc aacatcaaaa atagtgaacc aatcttggct
    56281 tattataaag aacaaggttt gctaaacact attgatggtg ctcgtgaaat cgatgctgta
    56341 tttgcagatg tacaaaaaat cattgaaaaa tagcacgatt gcccgtagcc gacttgcatt
    56401 atgcagttac tcttgtcaag ttcaataaat tatgatagaa tatgtcagtc cgacttctag
    56461 agaatcctct agatgatcca ttttgagaat tttgaggtgg gaactgcagt ttccgccatt
    56521 ttatcgtgtg aaaatgcgaa acaagtacaa ggaggtactg tgcgtggcaa aagaagatat
    56581 gattgaagtc gaaggtacag tcgtcgaaac tttgccgaat gcaatgttta aagttgaact
    56641 agaaaacgga caccaagttc ttgctactgt ttcaggtaaa attcgtatgc attacatccg
    56701 tatcttacct ggtgacaaag taacggttga attgtctcca tacgatctta accgtggccg
    56761 tattacttat cgctttaaat aattgtactc cgtaaaacta caggaggtat tatcatgaaa
    56821 gtaagaccat cagtaaaacc aatgtgcgaa cattgtaaag taattcgtcg taaaggacgc
    56881 gttatggtga tttgcccagc aaatccaaaa cataaacaac gtcaaggata attggaggtg
    56941 ctataaatat ggctcgtatt gcaggagtag atattcctcg tgataaacgt gtagtagttt
    57001 cacttactta tatctatggt attggtaaca ctactgctaa aaagatctta gctgatgctg
    57061 gtgtatctga agatgttcgc gttcgtgaat taacgaatga acaaacagat gctatccgtg
    57121 cagaagtaga taaattaaaa gttgaagggg atcttcgtcg tgaagtgaac ttgaacatca
    57181 aacgtttgat ggaaatcggt tcttaccgtg gaatccgtca tcgccgtgga ttgcctgttc
    57241 gtggacaaaa cacgaaaaac aatgcacgta ctcgtaaagg cccagctcgc gctatcgctg
    57301 gcaagaaaaa ataattctta agtgaaggag gttaaaactt catggcagca aaaaaagtga
    57361 atcgtaaacg ccgtgtgaag aagaatatag aagcaggtat tgcacatatc cattctacat
    57421 tcaataacac tatcatcatg atcactgata ctcacggaaa tgcattagcg tggtcatcag
    57481 ctggttcatt aggtttcaaa ggaagcaaaa aatcaactcc ttttgccgct caaatggcag
    57541 cagaaactgc tacaaaagca gcaatggaac acggattaaa gactgttgaa gtaacagtta
    57601 aaggacctgg ttctggacgt gaagcagcaa ttcgttcatt acaagctaca ggtttagaag
    57661 tgactgcaat tcgtgacgtg actccagttc ctcataatgg tgcccgccct ccaaaacgcc
    57721 gtcgtgttta atgagtgccg ctcattttga gttttatcag taacgtcatt gaacaagaca
    57781 cttttcgttt tgaaaggggt aaagataaga atgattgagt tcgaaaaacc aagaatcgca
    57841 aaaattgatg aagaaaaaga ttatggcaag ttcatcgttg aacctcttga aagagggtat
    57901 gggactactt tagggaattc cctacgtcgt attttattat cttctttgcc aggtgctgcc
    57961 atcactaata ttcaaatcga tggtgtactg catgaattct ctaccatcaa aggtgttaga
    58021 gaagacgtca ctcaaatcat cttgaacatt aaaggcttag cgctgaaaat gtatggccaa
    58081 gaagaaaaaa cccttgaaat cgatattacc ggacctgcta cagtaactgc tggcgatatt
    58141 atcgttgata gtgatgtaga aattcttaac aaagatatgt acatctgtag tgtctctgaa
    58201 ggagctacgt tccatgctcg cttaacagtg aaacctggac gtggttatgt tcaagcagat
    58261 gaaaataaaa aagaagatat gccaattggc gttcttccag tcgattcaat ctacacacct
    58321 gttcgtcgtg tgaactatca agttgagaac acacgtgttg gacaccgtga agattttgat
    58381 aaattgacga tggagatttg gacagatggt tcaattgaac cgttggaagc aatgagtttg
    58441 tctgcgaaaa tcatgactga acatttggat atttttgtga acttaactga tgaagcaaaa
    58501 aatgctgaaa tcatggtcga aaaagaagaa acacaaaaag aaaaaatgtt ggaaatgacg
    58561 atcgaagagt tagacttgtc tgttcgctct tacaattgtt tgaaacgtgc aggaatcaat
    58621 actgtacaag aacttacaaa taaatctgag cctgaaatga tcaaagtccg taacttagga
    58681 cgtaaatcat tagaagaagt taaagcaaaa ttgcacgact taggtttagg tttacgtaaa
    58741 gaagattaaa accttttacg aaggagggaa accaacgtga gttatcgtaa actaggacgc
    58801 acatctagcc aacgtaaagc gatgttgcgt gatttaacaa ctgatttatt aatcaacgaa
    58861 cgtattgtga ctactgaagc tcgtgcgaaa gaagttcgct caactgcaga aaaaatgatc
    58921 actttaggta aacgcggaga tttgcatgca cgtcgtcaag ctgcggcttt cgtacgtaat
    58981 gaagttgcca gcgtgcgtga agaaaatgag gacatcgtca ttgaatcagc tttacaaaaa
    59041 ctgttcaacg atattgcacc tcgttacgca gaacgtcaag gcggatacac acgcattttg
    59101 aagacagaac caagacgcgg tgatggtgca ccaatggttg tcttagaact tgtctaaaat
    59161 cacactaata acatcacttt atagatgatt cttttagagt gttatgatgt tggaagatat
    59221 ttttccgagt ctagctcagc gctgctcctc aagcgaatat cgcttgggga gtaagcactc
    59281 atcataaagt gtttttttat accttcaatt acctgtcatg acagatatgt ttgaaggcat
    59341 aaaaaaacat tttaagcgag aaataattga aaattttgtc tttttttgat agtgttagta
    59401 ggaactttag gatgaagaag gagtaatcat ggagccaatt attgaattag aaaagataaa
    59461 ttataaatac cagccagatg atcttcgccc cgcattgaaa gatgtctcct ttacaattga
    59521 taaaggtgag tggatcgcta ttattggaca caatggttca ggaaaatcga cacttgccaa
    59581 aacgatcaat ggattgcttc tgccagaatc agggatcgtg aaagtgggaa atcaaatatt
    59641 ggatgaagaa aatatttgga cgatccgaca gatggttgga atggtttttc aaaatccaga
    59701 caatcagttt gttggttcga cagtagaaga tgacgttgct tttggtcttg aaaatcaagg
    59761 gattcccaga gaagaaatgc tagtgcgtgt gaaagatgca cttgagaaag ttagaatggc
    59821 tgagtttgct tcacgcgagc ctgctcgttt atcaggaggg caaaaacaac gtgttgccat
    59881 tgccggcgtg gttgccttac gacctgatat cattatttta gatgaagcaa ccagtatgct
    59941 agatccagaa ggtcgtgaag aagtgatctc tacgatcaag aagatcaaag aagaaagtca
    60001 attgaccgtt atatctatca ctcatgatat tgatgaagca gctaatgcca atcggatact
    60061 agtgatgaga caaggcgaac ttgtccgtga aggtacacca aaagaaattt tttctgctgg
    60121 tcctgaactg atcgacttag gtctagatct accatttcct gaaaaactga agagcgcact
    60181 gaaagaacgt ggggttgatg tgcccagtga atatatgaca gaagaaagga tggtggattg
    60241 gttatggaca tccgttttga acaagtagac ttcacttatc agccgaatac accatttgaa
    60301 caaagagcct tatttgacat caatatgacg atcaaagaaa acagttatac tgcattagta
    60361 gggcataccg gaagcgggaa atcaacctta cttcagcatt tgaatgcgct tgttaagcca
    60421 acaagcggta cagtccatat tggagaacga gatattcagc cggatacgga taataagaat
    60481 ttaaagccca tccgaaaaaa agttggcatc gtctttcagt ttccagaagc acagctattc
    60541 gaagaaacgg tagcaaaaga cattgctttc ggtcctaaaa actttggtgt cagcgaagaa
    60601 gaagcactag tcctagcaaa agaaacatta gaacaagttg ggctggatga aagctatttg
    60661 gaacgttcgc catttgaact ttcaggaggg caaatgcgta gagtagcaat cgctggtgtg
    60721 cttgccatga ggccggaagt acttgtattg gatgaaccaa cggcaggact tgatccccag
    60781 ggcagaaaag aaatgatgga gatgttttgg cgtcttcata aggaacagca gatcactatt
    60841 gtattagtta cgcatttaat ggatgatgta gccaattttg cagattatgt ctatgtgttg
    60901 gaaaagggac gaatcgttaa tagcggggag ccgcaagagg tctttcaaaa cattgaatgg
    60961 ctaaaagaaa aacaactagg cgtacctact gccaccgagt ttgcagaaga attaatggct
    61021 aaagggatga actttgctac cttgcctttg acagctgaag aattagcaga cgctatcgtc
    61081 cattatgctg gaggaaatgc ccatgatgaa taaattgata tttggtcgtt atatgcctgg
    61141 agattccttg atccatcgat tggatccccg agctaaatta ttagccagtt tttattttat
    61201 cggaatcatt tttttagcaa ataattggga aacgtacctt ttgttggcat ttttcacatt
    61261 actttctgtc ttcttgtcaa aagtagattt gaaatttttt atccgaggca ttcgcccatt
    61321 gatctggcta atcctcttca ctgtagcttt gcaggtcttg tttacaagtg gcggagaaac
    61381 ttattggagt tggggtgttt tccatatcac tgaatttggt gtgatgaacg gcctctttat
    61441 tttctgtcga ttcgtgttga tcatttttat gtcgactcta ctcacgctta ctacaccgcc
    61501 tttggaatta tcggatgcga ttgagtactt gcttcgtcca ttgaaagccg tacggttccc
    61561 agtccacgaa atttcattga tgctgtcgat tgctcttcgt tttgtgccta cattgatgga
    61621 cgaaacagaa aagatcatga acgctcagcg cgctcgcggt gttgattttg gtgaaggaaa
    61681 cctagttcag aaaatgaaag cagtcattcc tttgctgatt cctttatttg tcagcagctt
    61741 caaccgtgcg gaagatcttg cgactgctat ggaagcaaga ggctacaaag gtggggaagg
    61801 aagaacgaaa tatcgtgtat tgcattggca taatagagac acgatggtta tcattgcttt
    61861 tgtattatta actgtgctac tgatcttttt aagaggataa acgttcagtg ggccgctatc
    61921 tattcttttc ttgctgatct gtttgcaaga aaggatgcaa gcagtgtatg acaagaaata
    61981 ttagggtctg tggcagacta aggtcacagg cctctttctt ttatctgaaa agaaagaacg
    62041 aaaaaatttt atagaaaaat cattattcca gataaccttt taggaacaaa ggagagaaaa
    62101 acatggtacg ttacaaagca atcattgcgt acgacggaac aaattttaat ggattccaaa
    62161 aacagccaaa tgggcgtact gttcaagaag aagtagaaaa aacattacaa aaaatggcaa
    62221 atggaaaaga aatcacggtc tttggttcgg gaaggacaga tgccggagta catgcgatcg
    62281 ggcaagtaat ccattttgat tatccagagg agcgtccgtt agaacgaatg cgtttcgcgc
    62341 tagataccca gtcaccagag gatattgctg taagacaagt agaaattgtt tcagaagact
    62401 tccacgcaag atatttggtc aaagaaaaga cgtatcaatt tcgagtggat atagggaaac
    62461 cacgcagtcc ttttcgacgc cattatgcaa gttattttcc ttacccactt gatttggaaa
    62521 aaattcagcg ggcgcttcct gatttattag ggactcacga tttcacctct ttttgtgcat
    62581 caggaagctc gatagaggat aaaatacgta cgatctacga ggctaagatg gaggtaaatg
    62641 aaacaaaaga cgaattgttg tttacattta ggggaaatgg cttcttgtat aagatgatac
    62701 ggatcttagt aggaacgctt ttgaaaattg gcaatggccg attgcctgaa gatagtatcc
    62761 cagatattat tgcgaaaaaa gatcgtaatg cagcaggacc tacagcacac cctgaaggac
    62821 tttatttgta tgaagtgaaa tatgaataag aggttatgat agaagtggtc agcttcaaga
    62881 aataagaagg tatttacgaa aattacttct caaaattttg caaaatttcg gcttattttc
    62941 gaagaagcta cttctggaac accgtttaag aaagaggtcg tgagcctgtc atttaactct
    63001 gagtcacaaa gagcaatttc aaaaaacagc ttctcatctt tgatgaatat cgtgacttgt
    63061 ggacgaggcg ttgataagtg aatcatcttt ttgttaccta ttttttctgt aaccaaaaaa
    63121 cgatggaacg ttgttttgat tgcccttggt gcggacagtc tgaacaggtt ccatcgtttc
    63181 tttattttct acgtcttctt ctgtgtgtgt ttcgtggttg ttcttctatt gctggatcaa
    63241 tcgtgccata aaaggggcga ttgcggtcaa ggtaccagac ataaagaata aacaatgcaa
    63301 tacataggac aaaaaggatt gcacctatcg taaatccttc aggcaagtaa ctgaaagtga
    63361 cagtatgttt acctgctgga acttttatca tcaagaatgc gtctttgaaa ttgctgacct
    63421 tgactggttt cccatcaatt ttagctttcc atcctttgtc ataaggaata gttgtcacca
    63481 acatggaatc ttcagcggta tttacagtcc cagaagcttt tctgccagtg gcttccattt
    63541 cgacaccttt ttctttgatc tgattcattg cggcttgata ggctacaaca tctagaccaa
    63601 cgactttagg ctccatgaaa cttatttctt tcgtcccata aaaacttgca gtaaatgtca
    63661 ctgttgtctc ttgaggataa tagccaagat catagtattg accagaaatg ttgatttgcg
    63721 tttttcgcga tgtaccattg acagtgatcg tcgcactaga actttctaat tgcgcaaaat
    63781 tggttgggaa caaacttaag taagcttgtg tatttgcagg cacatggact tcccaagtga
    63841 gctcttttgg ctggttatct gctgtttctg agtaagtgac gccagtcgtt gtctttgtga
    63901 tcgtcgtatt caactgattc ttcaaagtag gtgtatagaa tgtaaaatac tgttcattta
    63961 gaccagacaa tgtattgatc aagtttgtct gactggtcaa attatctgtc actggctgct
    64021 caacatcatt aacggacaac ggtgcagtaa atgctagtgg caatgcattg acattctgat
    64081 acagtgtata gtcgccgctt tgaccagctt ggcggaagcc atatttactg aacgtgcttt
    64141 cgtttttaga aatgttatat ttgattcctg taaacccatc catcagcaaa gtattattgt
    64201 tataacggat attcaaattt gtccctcttg aacggaaacc taatttatcc aggtaagagg
    64261 aagaattacg atttcttata gaagaaaaca aactgatacc actgtaacca tagttgatcg
    64321 aatcgtttgc agatactgga tccagatttt ccaagcggta aaacgagtcg gaatcttttt
    64381 ttgtttcatt tacaagagct ttcaagtcag gataaggatc agtatataaa ctgcgggaag
    64441 cataattcca atcatcaaga atgccatgga tcatactgtt cgtattgacc agtgcttcag
    64501 cagacatgat caataataac aatatactca gatgattgaa tgaaagtttt ttatgctgat
    64561 aaaatcccac agcaaaagca taaatcccta agaaaaccac tgtcaataca aaggaagtca
    64621 tcgtcacata ggtgtagctt ccttcaggtt tgatgcccca tgccaccgca aagagagcag
    64681 ctacgaccaa aatgattcct aacaacagcc cacgatcgtc ttgttctatc ttttcccatc
    64741 cttttcctgc aagcaggagc actaagaaag agaacagata ggcataacgg aataaaaaca
    64801 tattcggtgc atgcatacca tgccagaaca aattcaatgg aacaagataa aaacttgcga
    64861 taagtatggc gaaaagactg ccgtacaaaa atttattttt caacggtact ttccgactgg
    64921 caaaataaaa cagacagagg actaatggta gcagacctgc gtaaacgaac ggaatcgatc
    64981 cgtattttgt cgtgtcataa acaccaatca tatttttcat gattagatcc agataagaag
    65041 tcgcttctgt tttcaacgta gtgatctgac tcagtgcctc gccatttgac cgaagatcca
    65101 gcactgctgg caatacaatg atcattgaag cgccgcctgc cagtaaagaa gtgataccat
    65161 aaggaacaat cgttttttta tattctgacc agtttgtcaa tagacgggcg ataaaatata
    65221 agaaagaaaa tacgccaatc ataaaaccca tataaaaact ggataaaaac aagagtaaat
    65281 aactgataaa taaaagtttc ggccgtttaa gatccataac acgatgaatc cccaaaatca
    65341 ctaatggaag ataaacaaat gcatccagcc acatgatgat ctccgaatgt gcagtgataa
    65401 atgagatcaa tgcgtaactg acgctaagtg caacatgccc ccagcgagga atcttgaacg
    65461 tacgtgcaag aaaccaaaaa gccaatccgg cacagccgac cttaagcaaa gtcagaacat
    65521 acagtgcatc tggcatcatc tggtttggaa aaaagaacac cagtggcgtg aagatcccgc
    65581 ctagatagta agaaattaaa gataaataat tcaatcctag agaagcattc catgtataaa
    65641 aaatactttg ctttccaaga agaacatttc ggaagctggc atgaaaatta gaaaactgtg
    65701 aaaaggcatc acttgccaaa atacttctgc tgcttccagg atagattccg atacttagat
    65761 aaacaagtgc taaaatcaaa aacggaatca ggaaactagc tgctgcgtag cgtccgtttt
    65821 ctctaaagaa tgactttaaa ttttttttca tatttatttt ccaccaaatt tctatgaagt
    65881 tgtattcagt ttatcatatc cttgtttttc tctgcttaaa aagagtctag ttttcacaaa
    65941 aaaataaaat ttagaataaa taacttcata atttgttcgt ataacaaaaa taaacgcaaa
    66001 aaaagggtag gaatcaaaag tcccaccctt aaaaattttt attaaataaa aaaccgttca
    66061 ctatctgaac ggtccaagta tcattcaatt aaacgcgttc aacttttcct gatttcaaag
    66121 cacgagttga cacccaaact tttttaggtt ttccgtcgac cataacgcga actttttgta
    66181 agttaggttt aacagtacgt tttgttgagt tcatagcgtg tgaacggttg tttccacttt
    66241 ttgtcttgcg acctgtaaag taacaaactt ttgccatggt aatttcctcc tttgccttga
    66301 tttggagcaa tagaataatt cagctcatac cagactaaca tatcatatag aatgggaatt
    66361 tgcaagtaca ttttcataga tacaattgac aattgagaag aataattgta aattaaacaa
    66421 ggttatacct gaaattttgc aaggtttttg agtactttgg gtaactgcct tttatttcaa
    66481 gttgcgctat gataaaatat gatagaagaa aaatgggagt tttttacccg ttaaggaggc
    66541 tcattcatgg ctgtaaaaat tcaaacgtca gctggaacca ttgaaattac caatgatgta
    66601 attgcaacag ttgttggtgg cgccgcgaca gatgtgttcg gtgttgtagg aatggccagc
    66661 aaaaatcaaa ttaaagataa tattactgag attttgcgta aagaaaatta ctcaagagga
    66721 gtagttgtcc gccaagaaga taatggtatt gctgtagatg tttatacgat tatcagctat
    66781 ggtacgaaaa tctctgaagt ttcccgcaac gtccaagaaa aagtgaaata caatctagag
    66841 gcattattag gcgtaactgc caattcagtt aatgtctttg tacaaggtgt tcgtgtactg
    66901 ccagactagg caggagactg gctggtaact cagcagtcga tctgaaggag gattatctag
    66961 gtgagtgtaa cagaaataag tgcaagccag ttccaggaaa tggtccaggc aggtgcgaat
    67021 cgtctgcaag taaatgcaga atacgtcaat tcattaaatg tattccctgt gccagatggc
    67081 gatacgggaa caaatatgaa tctatctatg actagtggag ctaaggcagt agtagattca
    67141 acctcagaaa aagtaggaga attagcagca atgctttcaa aaggattgct gatgggtgcg
    67201 cgcggaaatt ccggcgttat tttgtctcaa ttgtttcgtg gattttctaa gcaaattcca
    67261 gatgttgtct cattaaatgc ttctgatctt gctgcagcct tcactcatgg agttgaaact
    67321 gcgtataaag cagtaatgaa accagtagaa ggaacgattt tgacagtagc tagagaagca
    67381 gcgaaagctg gagaaaagaa agcaaaaagt actgatgatg tcgttgaagt aatgacagct
    67441 gttgtaaaag gagcaaagcg tgctttagct aaaactccag acttgcttcc agtcctaaaa
    67501 gaagtcggtg tcgtagacag tggagggcaa ggactgctgt ttgtttatga gggcttttta
    67561 agtgcattga atggagaatt ccaagcggat gaaacatatg aaccttctcc agcagaaatg
    67621 gatgatatgg tcaatgcaga acatcatcgc agtgtccaag gtcaaatggc gacagaagat
    67681 atccattttg gttactgtac agaaatcatg gtaaaaatcg gagaaggacc tacagtcgat
    67741 agtacattcg attacgaaga attccgtaac tatttagatg gtataggaga ttctttactt
    67801 gtggtcaatg atgacgagat catcaaagtc cacgtgcata cagaaaaccc aggagaagtc
    67861 ttgaattatg gacaaaaatt cggttcatta gtcaaaatca aagtcgacaa catgcgcttg
    67921 cagcatgaga cgattttgga acacgatgaa gaagtgaacg agtttgcgaa aaaagaaata
    67981 ccacgtatcc cgtatggtat cgtagcgatc gctgctggta aaggcgtcca agagctgttt
    68041 gaaagtttag gtgctaatta tgttatcagt ggtggacaaa caatgaatcc aagtacggaa
    68101 gacattgtta aagcgattca agaagtaaac gcagataaag tgattgtctt gccaaacaat
    68161 aagaacatct tcatggcagc tgatcaagca gcagaagtag cagatatccc agtagcagtt
    68221 gttccttcaa gaacgatttc ccaaggattg acagctatgc tttcattcaa tgatcaagct
    68281 tctttagaag aaaacaaaga aacgatgaca gaaatgttgt ctagcgttgt gagcggtcaa
    68341 gtcactcacg cgatcagaga tacctcaatt gacggtgtag agatcaaaga aggagacttt
    68401 ttgggcatga tcgatggaaa aatcctgatc tctaacccag atatcacagc aacagcgtta
    68461 agtacactag aacaaatgat caatgaggac acagagatcg taacgatctt ggcaggtgat
    68521 gaaggaacag ttgaacaagc aaatgaattt gctgagaaac taacagaaaa acatcctgat
    68581 ttggaaatcg agatccacca aggagatcag cctgtctatc cttatctatt ttctgctgaa
    68641 taatttctgt gtcagaatat ctttctaaca cagtattcat tcataaaagg ccttcggtta
    68701 gtgatcaggt atgatcgctg accaagtcct ttttttgtta aaggagggag gaaaaattta
    68761 tgcgttcttt agatgatcca atcactatgc tggcaggagt cggagaaaaa agagcagcta
    68821 gtcttgccag cttgggcatc caaacgattg aagacttact gacttattat ccttttcgct
    68881 acgaagatat tcaggaaaga aatctaaacg agatccagga tcaagaaaag gttactttga
    68941 aaggaatcgt agtctcgcct cctgtgatga atcgatttgg ttataaaaaa agccgattgc
    69001 aatttcgtat gatgcaagaa catgatgtgt tcaacgtttc tttttttaat caaccttatc
    69061 taaaagaaaa agtcatttta tcggaggaaa tcgccgtata cggtaaatgg gatgcaaagc
    69121 gtaaagcctt gaacgggatg aagattttag gttctcaaac aatggatgac ttttcgccga
    69181 tatatcacgt caacaaatca attcgtcaaa cgacattagt tgatctaatc aaaaaagggt
    69241 ttcaagaata cggcacattg attgaagaaa atctgccaaa caaccttgtg gagaaatatc
    69301 gactgcttga ccgagcttct gctgtaagaa gtatgcattt tccaaaagac cacgaagaaa
    69361 accatcaagc caaacgacgt gtcgtttttg aagagttctt tttatttcaa atgcgtcttc
    69421 aaggattgaa aaaagcagaa aaagcagaaa cgaatggttt ggagatcctt tatgatgtcc
    69481 aaaaattgaa acaatttact caaaagctac cttttgaact gacaaatgca caaaaacgtg
    69541 taacgaatga aatctgtcgg gatcttcgca gtccgcagca tatgcagcga cttttgcagg
    69601 gagatgtagg tagcggaaaa acagtggttg ccgcgattgc gttatacgct accgttacag
    69661 caggtttcca aggtgcgttg atggtgccta cagaaatctt ggcacaacaa catatggaaa
    69721 gcttgcaaca gctgtttgat cccaatgaag taaccatcgc attgattacg gggtcaacga
    69781 aagcaaaaga acgacgtgag ctccttgaaa aactagagca aggggaaatt aacgtcgtga
    69841 tcggcacaca tgcattgatc caagatggag tcgaatttca gaatttaggt ctagttatta
    69901 cggatgagca gcaccgtttt ggcgtgaacc agcggaaagt acttcgtgaa aaaggctggc
    69961 gaccagatgt cttatttatg acagcgactc cgattcctag aacattagct attacagcat
    70021 atggtgagat ggatgtatct atcattgatg aacttccagc tggacgtatc ccgatcgaaa
    70081 cacgctgggt aagaacacct caattggaca gtgtactgga ttggacgaaa aaagaactgg
    70141 caagaggaca tcaaatgtat gtcatctgtc cgctgattga agaatcagaa gcattagatg
    70201 taaaaaatgc agtggaaatc tatgaaaaac ttcgtgaact atttgcacca aaatatgaag
    70261 ttggcttgtt acatggtaaa atgaagaatc aggaaaaaga agtaatcatg gaagccttca
    70321 aagacaacca gatgcagata ttagtttcga ctactgtaat tgaagtaggg gtaaacgtgc
    70381 cgaacgcgac cgtcatgctg atcatggatg cggatcgatt tggattggca cagctgcacc
    70441 aacttcgtgg acgtgttgga cgcggaagtg atgcttccta ctgtatctta gttgctaatc
    70501 ctaaaaatga actaggtgtg gaacggatga aaatcatgac agagaccacc aatggttttg
    70561 tactaagcga aaaagatctg gaattacgtg gtcctggtga agtatttggc ttccgtcaat
    70621 ctggtctgcc tcaatttgcg attgctgact tggtgacgga tggaaatgtt ttggaggtag
    70681 ccagagaaga ggctgcagcc ctttggcaaa taaaggattg gcagatgctt cctgagtatc
    70741 agcctttagc gcaaagcctc caaatagaaa atcaggataa tcgttatttc gattagcaga
    70801 ctgtgctata ctttatactg atgacggaaa aaggagtgga aacaatgaaa attgccgtag
    70861 atgcaatggg cggagatcac gcacctcaag caattgtaga aggcgtggct ttagcgcagc
    70921 aagattttcc agaagtagaa ttcttacttt acggaaaaga agcagaaatc aagaaatact
    70981 taacaaatga aaaaaacatc acgattatcc atacagacga aaaaatcaat agtgatgatg
    71041 agccagtaaa agcaattcga cgaaaaaaga ctgcatcaat ggtccttgct gcacaagcag
    71101 tgaaaaacgg tgaagcagat gctgtatttt cagcagggaa tacaggcgcg ctactagcag
    71161 cagggttgtt catcgtagga cgtatcaaaa atatcgaacg ccctggcttg atgtctacct
    71221 tgccagtagt agggaaagaa ggtgccggtt tcgatatgct ggatcttggc gctaacgcag
    71281 aaaacaaacc agaacatttg ttgcaatacg gtattttagg ttctttttat gcacgtaaag
    71341 tccgaggtat cggacgtcct cgtgtcgcac tcttaaacaa cggaacagaa gcaacaaaag
    71401 gtagtgaagt gacgaaaaaa gcctatgaac tccttcagaa tgaaacaagc ttgaatttta
    71461 ttggaaacgt agaagctcgt gagctactca atggtgtggc tgatgtagtg gtcacagatg
    71521 gatttacagg caacgctgtt ttgaaatcta ttgaaggaac agcaatgaat atgatgaatc
    71581 tattaaaatc tgctattcta aatgaaggga tcaaaggaaa aatgggcgct atgcttttaa
    71641 aagacggctt acgttctttg aaagcagaaa tggattattc caaacatggc ggtgccgttt
    71701 tgtttggctt gaaagcacca gtcattaaaa ctcatggagc aacaggcccc gatgctgttc
    71761 gctatacgat ccgccaaatc cacacaatgc ttgagacaga tgtagttgga caattagtcg
    71821 aacaatttga aaaaagcgaa gaatagtatt tggatatctg aagcgatccg ttccattgaa
    71881 aaaaaggtgg acattcctga aaaatgccag taaaattagc catggtgtag gtaattgaca
    71941 agtgtatgat tcttggaggt gaaaaagttg actcgcgaag aagtatttaa caaagtagcg
    72001 aaaattattt caaatcattt tgagatagat accgataaag tgacggatga attgaacatc
    72061 aaagatgatt taaaagctga ttcaattagt attatggaat tcgttttaga gcttgaagat
    72121 gaattcggta cggaaatttc agatgaagac gctgaacaaa ttgaaacagt cggtggcgca
    72181 gtagattata tcagcagcca tcttaaataa tttcaaataa aagcaaagaa ggataaaaag
    72241 ctgcatgcag ctttttatcc ttcttttttt aatctttttg acacgtaaaa gtcatttttc
    72301 tgataattct ggcgataaac agaaattatg ttgcaaagta aagcggtttt ctatataatt
    72361 attaataatt cattacacaa agataagaat aacctttgtt ataaaacgat ttattctgtg
    72421 taatgtcctt ttttattaag aaaaacttcc atttaggaag cagagagggg tattcgattg
    72481 tccgataacc aattattaga agttcagaac ctacatacag gattccgact caaagatgac
    72541 tattacgatg cagtagatga cgtttcattt actttagaca aaaacgagat tttagcaatc
    72601 gttggggaat ctgggtgcgg gaaaagtacc ttggctacta cgattatggg attacttgat
    72661 cctaataaca caaaaattac aggtgagatt ttatataacg atctgaattt aatcgacctg
    72721 aacgaaacgc tctataataa aatccgcgga aatgatatcg gaatgatttt ccaagatccg
    72781 ttgtctgcat taaatcctct catgagaatc gaagaccaga tcaaagaagg actttcctat
    72841 cacacaaaga tgaatgcaga acagcgtcaa gctcgtgcac tagaattgct agaacaagta
    72901 gggattccta atcctgcgag agttggacgc caatatcccc atgaactatc aggcggtatg
    72961 agacaacgtg tcatcatcgc aatcgcaatc gcctgcaaac ctccaatcgt cattgcagat
    73021 gaaccgacga cagccctaga tgtaaccatc caagcacaga ttcttgattt actgaaagac
    73081 cttcaggaag aaacaaaaac agggatcatc ttgatcactc atgacttagg agtcgtagca
    73141 gaaatggctg atcgagtggc agtgatgtat ggcggacagt tcgttgaagt agcaggtgta
    73201 aaagaactgt ttgatcatcc taagcatcct tatacacgct ctttactgaa ctctatacca
    73261 caagaaggaa atcatgatgc agaactccat gtgatcgaag gtgtcgttcc ttctctgaaa
    73321 aacatgcctc gtaaaggttg tcggtttgca ccacgtattc catggatacc agcaagcgat
    73381 catgaagaag agcctatcct tcatgaagtc gcaccgaacc acttggtacg ttgctcctgc
    73441 tataagcatt tccactttag agacgaaaaa ggagaggtgt agatgacaga attaatcaca
    73501 atcaaagacc tgaaagttca ttaccctatt cgcagtggat tcttcaatcg agtaacggat
    73561 catgtatatg ctgtagatgg tgtggatttt attatcgaaa aaggaaagac atatggttta
    73621 gtgggagagt ctggctctgg aaaatctact acgggaaaag ccgttgtggg attagaaaag
    73681 gtaacagccg gacaaatcat gtatgaagga aaagatgtaa cgaagcgaag caaccgcaaa
    73741 aaaatgggtt ataacaagga tgtgcagatg atcttccaag attcaatgtc cagtttgaat
    73801 cctaaaaagc gagtgttgga tatcattgct gaacccatcc gaaattttga acggttaagc
    73861 gatcaagaag aaaagaaaaa ggtaaaagga ctgctagata tcgttgggat gccggaagat
    73921 gcgctatata aatatcctca tgaattttca ggcggacaac gtcaacgtct aggagtagcc
    73981 cgtgctgttg ctaccaatcc aaaactgatc gtagccgatg aacctgtatc tgctctggat
    74041 ctatctgttc aagcgcaagt attgaatttc atgaaacgaa tccagcaaga atacggcctc
    74101 agctatttat tcatttcaca tgatttaggt gtagtaaaac acatgtgcga caatatcgca
    74161 attatgtaca aaggtcgatt tgtagaaatc ggtacaagag aagacattta taatgacccg
    74221 cgacatatct atacgaaacg tctgctttca gctattccgc aaatcgatgt agaaaatagg
    74281 gaaagccaca aagaaaatcg acgaagagta gaacaagagt atatccaaaa ccaaaaagaa
    74341 tactatgatg cgactggtcg agtctacgat ttgcgtacga tcacaactac acacaaagtg
    74401 gcgttgaaag atggaggtgc aagctaatgt ggaaaacgat tctgcgacgt gttttattga
    74461 tgatcccgca agttattatt ttaagtgttc tgatcttctt attagcccaa gcgatgccgg
    74521 gggatccatt taccggtttg atcaatccaa atcaggatcc ggcagtgatc gaacagatgc
    74581 gacaagcagc tggcttgaat gacccatggt atgtccagta cttccgttgg atcggaaatg
    74641 ctttgcaagg cgattttggt caaagtttct tatacaaacg ctctgttgct gacttgatcg
    74701 gcgaacggat catcaacact ttatatctat cgatcttgac agttgtcctt acttatttga
    74761 ttgctgtgcc tctggggatg cttgctggcc ggtatcaaaa ttcaatcttg gataagattg
    74821 tcgtaatcta caacttcttt agttttgctg ttcctttatt tatcttcggc ttgatcatgc
    74881 tatttgtttt tggttatcgt ttaggctggt tcccaacaag tggatcacaa acactaggtt
    74941 tgcaaggtgg atttatcact caatggatag accgtttgaa atatattctg ttaccatcaa
    75001 ttacccaagc atttttggcg actgccgtca caatccaata tctgcgaagt gaagtaatcg
    75061 attcgaaatc gctcgatttt gtcagaacag cccgttccaa aggggtaccg acgaataaag
    75121 tattcagtcg acatattttc cgaaatgcag cgttgccgat ggcttctcaa atggggtatg
    75181 aaatcacttc gttgattgct ggttctgtag ttatcgaaaa gatttttggt tatccgggag
    75241 tcggaaaact atttattgat tccatcggcc aacgggacta taccgtgatc acatccttag
    75301 tattgatttt aggaatcgcg acgctagtgg ggacattact ttcagatatc atcatgagta
    75361 tcgttgaccc gcgtattcgg attcaataaa accaatcaga aaggagaatg gatcgatgag
    75421 tgaaaaaaac aatacagcag ttcaagaaag tattcctcct atggggatac gaatgattgc
    75481 tcgagaattt aaaaaagata aaattgctat tttttcatta ggtttgctag tggttttatt
    75541 attagtgatc tttatcggtg cgatgctgac cgatcaagac aaagtgatgt atgttagtat
    75601 cttcgacaaa tacgctgcac caggaacagt ttcgacacaa ggacaaaagt tcattctggg
    75661 agcagatgaa ggcggacgtg atgtattagg acaattgatc ataggtgcta gaaactctgt
    75721 tacgatcgga tttgcgatca cgatccttac ttcgatcatc ggggttggat tagggatcat
    75781 ctctggctac tacggaggga tcattgacaa catcttgatg cggatcgttg attttatcat
    75841 gattctaccg atcatgctga tcattatcgt cttcgtatcg attatttcca gatacaatgt
    75901 ctggtccttt atattcatca tgagtgcatt ttattgggta gctaaagccc gattattccg
    75961 aagcaagaca ttatctgaag caagaagaga ttatgtcagt gcctcaaaaa caatgggaac
    76021 caatgatttt aaaatcatgt tccgcgaaat catgccgaat ctaagttcat taatcattac
    76081 taatttgacg atgaactttg cggcaaacat cggtatcgag acaactttga ctttcttagg
    76141 tttcggcttg ccggcaaatg tacctagttt aggtacattg atcggttatg caagtaacgg
    76201 agaagtctta gtgaatcggc aatggatctg gttacccgca tcaatcctta ttttagtttt
    76261 gatgttgagt atcaattatg ttggacaagc attcaagcgt tctgcagatg cgcgccaacg
    76321 gttaggctag tatagaagcg aagggggaaa taggaatgaa gaaaacgatt tttggatttg
    76381 cagcgctgat gtctgctgtc gttcttgcag catgcggggg cggaggtaca gatacctctg
    76441 gaaacaactc tggaggcaac ggcaataaca aagaggaatt aacttttcca attgcaacaa
    76501 aaaataatga tgatgcaatc gaagacggcg aattagaagc tgcggtcgca acggacagtc
    76561 agtttaaagg attgttccag tgggagtttt accaagattc atatgatgca gcttttatgg
    76621 ctccttctca tgaaccgcta tttgcaaacg atgacgattt cagaatcaca gacgacggag
    76681 ctgcaacgct tgatctagat gaagatacga aaaaagcgac aatcaaagta aaagacaacg
    76741 tcaaatggtc agatggtgaa gatgtaacgg cggacgatgt gattttccct tacgaaatca
    76801 ttgggaataa agattacact ggtatccgtt atgacgacac tttccgtaac atcgtaggta
    76861 tggaagaata tcatgatgga agtgccgaca caatctccgg aatcaaaaaa atagacgaca
    76921 aaacagttga aatcgaatat aaagaagtaa acccaagcat gcttcaatcg ggtggtggta
    76981 tctggtcata tgcagcacca aaacatacgt tagaaggtgt agcaatcaaa gatatggaat
    77041 caagcgatca agtacgtaaa aatccagtga cttttggccc ttattacatg agtaatatcg
    77101 ttgcaggaga atcagtagaa tacttgccaa acgagcacta ctggaatggt acgcctaaat
    77161 tgaaaaagat cacgatgaaa tctgtaccga tggcaagtgc aactgaagca ttgaactcta
    77221 aattatatga tatgattttc caaatgccaa cagatactta cgatacctat aaagaaattg
    77281 ctggctatac aaacttaggc cgtgaacaaa cttcttatac ttatctagga ttcaagttag
    77341 gtaaatggaa tgcagataca aagagtgtgg aatacaatcc agattcaaaa atggctgata
    77401 aatcattacg tcaagcaatg ggctatgcat tagataataa tgcagtaggt gaacgtttct
    77461 atgcaggttt gagaagcaat gcgacttcat tgattcctcc agtattccag tcataccatg
    77521 atgatgagat gaaaggattc acacaagatc tagacaaagc gaaaaaacta ttagatgatg
    77581 ctggctacaa agatgtggat gacgatggtt taagagaaga caaagatgga aataaactaa
    77641 caatcaactt tgcttcgatg tccggtgggg aaacagcgca accattggca gattactata
    77701 tccaacaatg gaaagaaatt ggtttggatg tccaatacac aacaggtcgt ctgatcgaat
    77761 tcaattcttt ctatgataaa ttagaaaacg atgatcctga aattgatgtt taccaagctg
    77821 cttggaatac aggaactgat ccaaatccaa gtggactatg gggaccaaat gctgcgttca
    77881 actacactcg ttatgaatca gaagaaaata caaaattgat caacgatatc agttcaagca
    77941 aagcatttga tgaagagtac caaaaagaag catatcaaaa atggcaacaa tatgcatttg
    78001 aagaagcatt tgcgattcca acgttgttcc gtaatgaagt attgccagtc aacgaccgtg
    78061 tcaaagactg ggattggtct tatgatgtac caaacccatg ggcaaatgtt tctgttacag
    78121 cagattcacg ttcataatca ctaaaaatga ccgaaaaagg gcgatgtcct ttttcggttt
    78181 tttgatattt ttctgtttat ttctctaata ttcaggtcaa ggcgctgatt tcatgacttc
    78241 tccaataaac ggtgttcaaa gtccagtttc tcggtattaa gtcaaattta tgcaaaaata
    78301 tggaaagcca ttttcacaaa attttttctt catactcgaa actaaacgaa tttttcacaa
    78361 cttctcgtca atcactagga ttattaaagc tttacttacc ttttgaccga accatttcaa
    78421 atttgacaaa aaaccagtat aataaggaag tatcgaaaaa atatccataa aaggaatgtg
    78481 gaaaatggac aaccagctga caaaagaact aaaagaaaac tataacatcg tttttcacga
    78541 tctaaatttg cttgaacaag cttttaccca ttcatcctat gtgaatgagc atcgcaatct
    78601 gcaattatcg gataatgagc gacttgaatt tttaggagat gccgttttag aactgatggt
    78661 ttctcagtat ttattccgtt tatatcctca tttgccagaa ggaaaattga caaaaacacg
    78721 agctgcgatc gttcgcgaag acagcttgtc gaaatttgcc aaagaatgtc actttgatca
    78781 atatgttttg ttgggcaagg gagaagagaa ttcaggagga cgtacgcgac cagcattgct
    78841 ttgcgaccta ttcgaagctt ttttaggcgc gttatatctt gaccagggat tcgacacgac
    78901 ccatgcattc atcgaaaaag tgatttttcc taaagtcaaa gcaggtgctt tttcacatga
    78961 gatggatcat aaaacaaaac tacaagaagt cttgcaaaaa tcaggtgatg tatcaatcga
    79021 ataccgattg atcaatgaag aaggtccagc tcatgaacga gtattttgga tcgaagttta
    79081 tgtagatgat caattgattg gcactggaca agggaaatcg aaaaaattag cagaacaagc
    79141 tgcggcggaa aatgcattgg cagcactgta aaagtaagtg agggaaagct ttgtatttga
    79201 aacgaattga aatagccggt tttaaatcat ttgcagacaa gacagtcatt gattttgaaa
    79261 atagcgtcac agcagtggtt ggaccaaatg gcagcggaaa aagcaatatt acagaggcta
    79321 tccgctgggt attaggagaa caatctgcta aaagtctgcg aggcggaaaa atgccggata
    79381 tcatctttgc cggttccgat acccgtaagc agctgaatat tgcagaagtc acagtgattt
    79441 tagacaatag tgatcattat ctgccattgg agtacaatga gatcagtgta acccgccgtt
    79501 atcgacggac tggggaaagc gagtttttca tcaataaaca aagctgcaga ttaaaagata
    79561 tccaagaatt gttcttagat tctggattag gaaaagaatc gttctcaatc atttctcaag
    79621 gaaaagtaga agcgattttc agtagtaaac cggaagaccg tcgtgggatc tttgaagaag
    79681 cagctggtgt cttgaaatac aagcagcgga aaaagaaagc agaacagaag ctttttgaaa
    79741 cagaagacaa cctcagccgc gtccaagaca tcatccacga attggaagaa cagttaactc
    79801 ctttggccgc ccagagtgag gcagcaaagg agtttttacg gctaaaagaa acactgactc
    79861 aaaccgatgt ctctttgatg gttgctgaaa tcaagacagc aaaaaaagat tgggacaaca
    79921 aacaagcaca gcttgcgaaa ttcaatctag aattaggcaa actatctgag tcgatccaag
    79981 aacaagaatc cattttggcg aagcagcgta aagaaaatgc gcaagccgat cgtctgatcg
    80041 aaaaaaatca gcaagtatta ttagatcttt cggaaaaact gaagcagaca gaaggacaaa
    80101 aagatgtttt gcaggagcgg acaaagcata cacaaaaaag ctcgcaagaa tatcaaactt
    80161 cattagcaga agcacaaaaa aaggttaaac attttgaaaa gcttcaagaa agcttgatga
    80221 aagcagcagc agaaaaagaa acagagatcc aaaaagctga agcaaatctc ataaaaacac
    80281 agcaagaatt agaaaaatac caaaaatcaa cgaaagaatt attggcagaa ttaagagatc
    80341 aatatgttga tttgatgcaa gaacaagcag ctgtaggaaa cgaactgaag tatctagaac
    80401 gtcaatatat acaagaaaca gcaaaaagca agcagacgtt agccaagcaa tcggaagtag
    80461 aagcatcagt agaccgattg atcttacaaa aagaagaatt gacacaaaag caggcgcagc
    80521 taaaatcatc tttaactgaa acaaaagaga agttagaaat gatccaacaa aacgggaaaa
    80581 agttccaaga agcattagca aaagaacagc ctaaaatgta tcaattaatg aatcaagtcc
    80641 agcagctacg tgctcgtaag aaaagccttc aagaaatcca agaaaactat tttggttttt
    80701 accaaggggt tcgattggtc ttgcagcata aacagcagct ttcgggtatt gtcggagcag
    80761 tggctgaact gatcgatgtt cctgcagatt tcacgttagc tatcgaaaca gctttaggtg
    80821 gggcagccca gcatgtcatc gttgaaaatg aaaaagatgc tcggcaagcc atcacgtatt
    80881 tgaaacagca aagaggagga agagcaacat tccttccact gacgacaatc aaaccaagac
    80941 aattaccggc tcatatattg acacaagcag cagctgtaga agggtttata gggattgcca
    81001 gtgaacaagt cagttatccc gatcaaatcc aaactgtggt tcaaaacctg ctaggaacga
    81061 tccttttagc caaagactta actagtgcca atgcaatcgc gcaaacgatc cgctatcaat
    81121 atcgagtggt ttccttagaa ggtgatgtga tgaatgccgg aggatccatg acaggtggag
    81181 cgaataaacg aggaaaccaa ggcagtttgt ttgtacaaaa tcaagaattg aaacagttga
    81241 ctagtgaatt tgaagaagca gataaacaac tccaagcaca agaaaaaaaa gttcaggagc
    81301 tgcaacaaga aacagctcgt ttagccgaag aacaagaagt tttgcgtaca cgaggcgaac
    81361 aattacgttt tgaagaacaa gaagcgacaa atcagctaca aaacatcata aatgaacttg
    81421 aacgttttga aaaagaaaag caaatctcaa cttttgagac acgtgaactg cagcagttta
    81481 tcgaagatta cgaaaagcaa acgaacgaat tgaaagacaa acaaacagat cttgaatcgc
    81541 aacgacaaaa aatcgacgaa gaaatcaaat cgttaagtca agaaagcgac cagatggaag
    81601 ccagaagagc acaagtacaa agtcaaaaag cacaagaaca agcagatctt gctgtcttga
    81661 aagagcagtt taatcatctg cagatccagc tccgcggtgc ccgagtccaa aaagcagagg
    81721 caaccgaacg tcaagaagcg atagaaaagc agttggcgac attgacagca gacttttctg
    81781 atcatgaagt cacagaagaa agtttggaaa aacaaattaa cgaattatct gctcagcgag
    81841 aaacattaaa agccgagttg gcaaaagcca aagagcagcg agatagaagg caaaaggaga
    81901 tcgataagct tgaagcagtc ttggcagagc gtaatcgtga gcaaaaagca cgactgtcgg
    81961 aacaatccaa attagaagtc caaaaagacc gagctgaaat gattttagat aatcatttga
    82021 cctatctgca gtcagaatat cagctgacat ttgagaaagc ttcccaagac tatcaagaaa
    82081 caaccgatat cgaagacagt agaacaaagg tttcatcctt aaaagaacag attgaaaaac
    82141 tgggaccagt aaacttaaat tcaatcgaac aatacgaaca agtcagtgaa agacatacat
    82201 tcttagcaac acagcgagat gatctgttag cagcgaaaaa ccaattgttt gaaacgatgg
    82261 atgagatgga tgatgaagtg cggacaagat tcaaagaagt atttgaagct atccgtcaag
    82321 aattcaaaat tgtctttcca aatatgtttg gcggcggtaa agctgaatta gtactgacgg
    82381 aaccaactga tttgctgaaa acaggtattg agattgaagt acaacctcct ggcaaaaaac
    82441 tgcaaagtct aagtttgctt tctggaggag aacgagcact tacagccatc gcgttgctct
    82501 tttccattat ccgtgtctgt cctgttccgt tttgtatctt agatgaggta gaagcagcgt
    82561 tagatgaagc caacgtgaaa cgttttggtc gttacttgag cgatttccaa gatgacacac
    82621 aatttatcgt agtcacacac cgcaaaggaa cgatggtcgc agcagatgta ctgtacggag
    82681 tgacgatgca ggaatccggt gtatcaaaaa tcgtttctgt acggatggaa gatatcaatg
    82741 aagaggcgaa aatcaacgaa acgaaagtgt aggaacgaaa atgatcaaat tagtagcaat
    82801 cgatttggac ggtacattat taaatgaaca aaagctgatt tcagatgaga ataaacaagc
    82861 attagcacaa gcaaaacagc aaggggtcaa gatcgttcta tgtacaggac gccccttagc
    82921 tgcgatggcg cattatctgc aagaattagg actggtagat gaaggtgact acagcatcac
    82981 tttcaacggc ggtttggtac aaaaaaatga tactggtgaa atcatggaaa agaaagtgat
    83041 ggaagtatca gatatccaac gcttatacaa actggctcag caattggatc tgccactaga
    83101 tgttttgtct gacaatgtgg ttttacagct gcctagtgcc ccaaggaagc aatccttgta
    83161 caatgtgttg aataagctgc tgcaattcca accagcggat ataagcgata ttacagatga
    83221 atttgtgctt aataaggcag taatcgctta tgaccaagaa gaattagatc ctaaaatcaa
    83281 agaaatacca gcagaattcc atgaaatgta tgagatcatc aaaacaagaa gtatgctttt
    83341 ggaatttatg ccaaaaggtg ttacaaaagc ttatggtatt tctttattgg caaaagatct
    83401 tggtttgaaa caagaagaaa tcatggcgat cggtgatgag gaaaatgatt taccgatgat
    83461 ccaatatgct ggactaggcg tagcgatgga gaatgctgtg cctttcgtca aagaagcagc
    83521 agatttcgtg acagccagca atgaagaaaa tggcgtagcc aaagtgatcc aaaaatttgt
    83581 attagagtag ggggagtcaa atgggatttt ttgataaaat caagcgtgct tttactggcg
    83641 aagagaaaac agaagaagtt gaagtacagg agaagtacga caagggttta gaaaagacgc
    83701 gtaaaacatt tggtgaacgg atgaatgagt tgtttgccaa ttttcgaaca gtcgatgaag
    83761 acttttttga agaattagaa gaaacactta tcggtgcaga tgtaggtttt gaaacagcga
    83821 tacgaatcac cgaagcatta cgtcaagaag tgaaactcag aaatgcgaaa aaatcggcag
    83881 aagtccaaaa tgcgatcatc gagaaacttg tggatctcta tgaagcggaa ggaatcaatg
    83941 aagtaaatga actaaatatc caagaagacg gactaacagt tattttgttt gtcggtgtta
    84001 atggtgtagg gaaaacaaca agtattggta aacttgccca tcagttcaaa aacgaaggta
    84061 aaaaagtatt gatggcagca gcagatacct tccgcgctgg agcaatcgac cagttagttg
    84121 tctggggcga acgcgctggt gttgaagttg ttcgtggcaa tgcaggaggc gatccagcag
    84181 ctgtcgtatt cgatgctttg gaaagagcaa agacagagca ggcagatgtt ttactagttg
    84241 atactgcagg ccgactgcaa aataaagtga acttgatgaa cgaattagaa aaaataaaac
    84301 gagtaatcca gcgggaatta ccagaagcac cccaagaaac acttcttgta ttagatgcaa
    84361 ccactggtca aaacgcgatg gttcaagcaa aacaattcaa agaaacaaca gatgtgaccg
    84421 gtcttgtttt gacaaaatta gatggtacag caaagggcgg tatcgtactt gctatccgta
    84481 atgaattgca tttaccagtg aaattggtcg gtcttggtga aggaatcgat gacttggagc
    84541 cgtttgatcc aaatgatttt gtcgttggat tgttcaaagg attactaaaa gaagaataac
    84601 ccatgtctaa aaagcaggag ctgtgacaaa agttgcagct ccttgtttga atgattgttt
    84661 ttaacatagc tgtgtttttg atgccttcat ttttatactt atttgtaagc ggttctcaaa
    84721 caatttcgtt ctttttctta gactttttga ttataattag attcatatga attggaggtc
    84781 acttaatgat gaattttaag caattattat tgcatgtcaa tgcgcgccct tttatcgacc
    84841 aagctcgatt tggtatagaa cgggaaggac agcgggttga tcttgcagga aatctagcaa
    84901 aaaccgatca tccagcaatc tttggcgatc gatcctatca cccctatatc caaacagatt
    84961 ttagcgaaac acaaacagag atgatcaccc ctgttaccga ttctattccc gaattatttc
    85021 agtatctcgc tgctgtttat gatgtgactg ctcgttctat accgaaagaa gagatgatct
    85081 ggccattaag tatgccacct gccttaccgg aaaaagacga agagatcatt attgcaaaat
    85141 taaaaaattt cgaagatgtc ttgtatcgac gttatttagc aaaagaatac gggaagcgaa
    85201 aacagatggt aagcggtatt catttcaatt ttgaatttgg cgatgagttg ctaagaacat
    85261 tgttcagcca tcaggaagaa tttcaagatt tttccgaatt caaaacagaa ctttatttga
    85321 aaacagcgag aaactttatg cgctatcgct ggatgatcac ttatttattc ggtgcttcac
    85381 caatgagtga gaagaactac tttttagatg aatcacatcc gcaagaacct gttcgcagta
    85441 ttcgcaacag tgcgctgggc tatacgaatc atccaaatgt gaaagtttcc tatgcttcta
    85501 tgaaacagta tttagcagat atcgagcgaa tgatcgaaga agggaaactt tcggaggaga
    85561 aagaatttta tacaccgctt cgtttccgag gcggaaagaa agtcgcagat ttagcaacaa
    85621 caggtgttcg ttatattgaa ttgcggaata tcgatttgaa tccttatgca agattgggta
    85681 tcaatccaga gcaagttcgg ttcttacaat tattcctgat gtacatgtta tggacagaag
    85741 aaaaagaaga ctgtgaccaa tgggtagcag aaggtacgac tcgaaataac aaagtagcac
    85801 tagaacaacc atcagatcaa acagaatttc atcaagaagg tagagaaatt cttgaaggaa
    85861 tgaagcaaat gctggttgaa ttggattggt tggattctct ttatttagta gaggaagcat
    85921 tgactcaaat ggatcatcct gaacaaacgt tagcagcgaa actctatcaa gaagcacagc
    85981 tatcgagtca gcaagaagtt gctgtggcat tgggacatca atattataaa gaaagccatg
    86041 aacgtcccta tcagttggcc ggttttcgcg aaatggaact ttctactcag atatttatgt
    86101 tcgatgccat ccaaaaaggt gtccaagtca aagtattaga tgagtcggat caatttttgc
    86161 gtctgcaatt ccaagaccat gtagaatatg tgaaaaatgc aaacatgaca agtaaggaca
    86221 gctatatcgt cccacttatc atggaaaaca aaacagtaac gaaaaaagtc ttaaaagaag
    86281 ctgggttccg ggtaccagga ggcgcagaat tttcatccat ggaagaagcg gtaaaagctt
    86341 atccgagatt tgccgagcag gcatttgtga tcaaacctaa atcaaccaat tacgggttag
    86401 gtatcacgat tttcaaggaa ggagctagtc tggaagatta tcaagcaggt ttagccattg
    86461 cttttcgtga agacagttcg gttttagtag aagaatttat gccgggaacg gaatatcgtt
    86521 tctttgtgat cgacggagaa gtccaagcca tcatgttgcg agtccctgct aatgttattg
    86581 gtgacagtat ccgaacagtg aaagaacttg ttgaagaaaa aaatagtgac cctttgcgtg
    86641 gaacgaatca tcgtgcacct ttagaattga tccaattagg tgaattagaa caattgatgt
    86701 tgaaagaaca agggttgacg atcgaatcag ttccccaagc caatcaaatt gtttatctaa
    86761 gggagaactc aaatatcagt acaggtggtg attcgatcga tatgactgat gaattttctg
    86821 aagcttacaa aaaaatcgcc gtttctgcgg tagaagcact aggagctaag attagtggta
    86881 tcgatttgat tataccagat aaagaaatcg atcctacaac tgataaaaaa gcatatggga
    86941 tcatcgaagc aaatttcaat ccagccatgc atatgcatgt ttatcctttt gcaggaaaag
    87001 gcagaagatt gacgatgaat gtattgaaat tgttatatcc tgaagtattt taaaagaaaa
    87061 agcttttgac agattgtcct atttgatgtc aatctgatca aaagcttttt ttgcatgtaa
    87121 aaatttatgt gttttttcgt tttaaaataa acgatcccag aagctttttt tcttttttgg
    87181 tttttctttt tcagctggcg aagagttttc ttctgggtat tttttttcga tatcaatcac
    87241 aggttcattt actgcttctt ttgctgttaa gagcaatccg aattcctcag gatttgcagt
    87301 gacgtaatcg ttgactacag tgaaacggct attgtttttc gttgctagct ggatatattg
    87361 gttttggaca gaaaggggta gagccccatt caagagtatg gaagcatctg gatgtttttt
    87421 gagttctttt aaaaagtttt ctttgttttt tggttggatc atttcagcaa cagtcatact
    87481 aagatagcag cgttccctga atgtccctaa atatttatgc tgttcttcag ggttcactaa
    87541 aggtgtacca taaagtccct tatccaaatg gctttttaca tcatcttgtg ccataaaaaa
    87601 taccctcctt cttctctttg tctttcttac gattatagca tattcccaat aatacctaaa
    87661 acatatgaaa aagcggttgc ttttgttcaa cgattcactt aaaataagaa tgaaaatcat
    87721 tctcaattag aaaggtggat tggatgcgct acggacgact agtagcatta ttagttattt
    87781 taagtagtat ttcattgttc atcggagtat cgaatgtaca attgtcagaa ctgctccagt
    87841 tagatgggca aagcttacaa atcttgctag tgactcgttt gccacgaacg atttgtttga
    87901 tccttgtggg agcgaccagt agtatttgtg ggttgatcat gcaacatttg acccaaaata
    87961 aatttgtttc accaacgact gcgggcacga tggatagtgc gcgtttaggt attttagtgg
    88021 ctatgatttt cctacctggt gcatccttga ttgtccgatc cttgacagca ttttgctttg
    88081 cttttgcagg aacattgctt tttttatccc ttacgcgatt gttcctccag aaaaatcaat
    88141 tgatgcttcc gttgattgga gtcatgttag ggaatatcat tggttcagtt gctacttttt
    88201 ttgcttatca attccagttg gtacaaaata tgacttcatg gcttcaaggg aattttgcga
    88261 ctgtgatacg aggaaattat gagttattgt atgctgttgt tccattgttg ctcttggttt
    88321 ggctatttgc ttaccgattt acgatcattg gtctgggcga atcgtttgca gtaaatctgg
    88381 gaatagatta ccagaagatg cagtttttag gcattggctt agtatcattg gctagtgcag
    88441 tcatgttgat catggtagga agcattcctt ttatcggagt gatcattccg aatcttgtct
    88501 ctagaatata tggagatcaa gtgcacaaaa caattggcgt aactgcttta gcaggtagtt
    88561 tgttcttagt attttgcgat cttgtcgctc gcgtgttgat ttttccttat gaaataccag
    88621 tcagtgtgat cgttggaatc atcggtgggg ctatcttcct ttatcttctg ataaggggac
    88681 gacgatatgc ttaaaaaaaa gccatctatt tttattggta tgcttgttct tgttttattt
    88741 gttacaggaa tgtacttgac gatccataca tatggaaact ggcattttgc atgtgcattg
    88801 aggagtaaga aaatttttgc atttatcctt gtctcgctag ctgtcagcag tgccacaatc
    88861 agctttcaga caattacgca taaccagttt ttgactcctt cagtattggg acttgagtcg
    88921 ttatatgtcc ttgtccaaac ggtccttttc ttctttatcg gaggagtgac gatgcttcag
    88981 cagacaggta cagttttttt cttattgaac gttgggctga tggtcgggct gagtctattg
    89041 ttatttttct ttcttttgaa aaaaatgaga gcgaatcttt ttttattact gatgtcaggg
    89101 atgattttag gtacgctttt tggtagtgtc agtacctttt tacaagtcat catggatccg
    89161 aatgaatatg atttgcttca aggcagatta tttgcaagct ttggcaatat ccagagtcag
    89221 tatcttttgc ctgcttcttt gatcattatc ggctgctggt acctgttatt tcgatatcga
    89281 catgtgctag acgttttcca tctcggaaat gaacaagcag taaacctcgg gatagacaca
    89341 gatcgttttg aatttttgat tttatgttgt gtcagtcttt tgacagggac atcaacggct
    89401 cttgtgggtc cagttacttt tttagggttt attgtcgcaa atatcagtta tcgtgtcttt
    89461 tctacttatc gtcatggtca gctatttatc ggaagctttc tggtagctat gctgtttctg
    89521 acaggaggac agctcgcagt agagcagatt ttcggctgga atacaacagt gagtgtcgtc
    89581 gttgaatttg tcggaggaat ttattttgtc acaaaattga ttcgcgaaag gaagagtatc
    89641 ctatgattga tttacaaaag gtcaataaac tgtaccatgg aaaatcagtg gttaaagatg
    89701 tacagttaac gattcctgaa aagaagttga ctgcttttat cggtccaaat ggtgctggaa
    89761 agtcaacggt gttgtcgatg atcagtcgtt tgataccaaa agatacaggt gaaatctatt
    89821 tagaccataa cgaagtaaag gcttggaaat ccaatgaatt agccaaaaaa ttatcgatcc
    89881 tacgacaatc gaatgaaata aatgtccaaa tcaccgtgaa agaattagta gaatttggac
    89941 gatttcctta tacgaaagga aaattgacga caaaagacga ggaaatcgtt caggaatcac
    90001 tggaacacct tgggttagaa gaaatagcgg atcaatcgat tgacactctt tcaggcggtc
    90061 aactccaacg tgcgtatatt gcaatggtgc tagcgcaaga cacggattat attttactgg
    90121 atgaaccttt gaacaatcta gatatgaatt ttgcggttca aatcatgcag attttaaaaa
    90181 atttagtaca agaaatcggg aaaacaattg ttattgttct tcatgatatc aactttgctg
    90241 caagttatgc agacgaaatt gttgcaatga aagatggaca gttatttacg caaggaacga
    90301 cccaagaaat gataaaaaaa gatgtattgg atgaattata cgaaatggac atacggattt
    90361 gtgagattga aggccgcaga ttctgtcttt atttctaaaa ctaacattag gagagaagag
    90421 atgaaaaaat tagtagtagg tatcttgatt ctagcatcat ttggtttagc agcatgtgga
    90481 aatagcaaca acaccagtca ggcagacaca aaaaaaagca gcacacaaac tgaaactaca
    90541 ttgacgataa cagatagtaa tggagaccaa atcgaggtgc caaataatcc agaaaaagtc
    90601 gtcgtattcg acaatggttc tttagatacg atggatgctc ttggtgttgg agataaggta
    90661 gttggagcag ctacaagcag ccttcctgag tatctttcat cctataaaaa agtagaatca
    90721 gcaggtggaa taaaagaacc agacttagaa aaaattaatc agcttcagcc agatctaatc
    90781 atcatttctg gccgacaaag agattttcaa aaagatctaa gtcaaattgc tccgaccatt
    90841 ttcctttctt tagatgcgaa aaatccatgg gaatctttcc agcaaaatgt cactgcttta
    90901 ggggagatct ttggtaagca agaagaagct aaaactcaat tagaggaatt atctagtgca
    90961 atcgatcaga cgaaaaagaa agcagaagct actgacaaaa aagcactagt cacacttgtg
    91021 aatgaagggc aattatcagc ctacggatct ggttctagat tcggtttcat ccatgattta
    91081 tttggctttg aacaagcaga tgatcaaatc gaagcatcca ctcacggtca aagtgtttct
    91141 tatgaatatg tgctggaaaa aaatccggat atcttatttg tagtagacag aacaaaagct
    91201 attggtggag acgacagcaa agatgatatt tctgccaatg aactggtagc acaaacgaat
    91261 gctggtaaaa atcagcagat catctccctt gaaccagatg tctggtattt aagcgggggc
    91321 ggtttagaat caatgaaact gatgatcgaa gatgtgaatc aagcatttaa atagcataaa
    91381 taacagagga ttaaactctc atactatgta acaataatgt ttcttacatt cttaaatagc
    91441 aagagcctat cctcaaagaa gttatcaaaa accatccgta aaatcgtatt gatgatttta
    91501 cggatggttt ttatttattt gagaaatctt ttttcaacgt gaattcatta tctacatcat
    91561 acgggttgat tctagaatgg ggagtggcaa aaaatagtgt gatccatttt ttcagcatca
    91621 agatcgcctc ctttgatttg aatcaaaggt acaaagcgaa acttagataa aacttaaatt
    91681 tagatatttt tattttgtac cgatctcttc tttaaacgat gttccatctg ctgcttctcg
    91741 gcaataagtc cactagacta gaaaatatga gaagctattt tctgtccagc gttcttatta
    91801 ctcaaagcaa atcgcagttg tcacaatctc ttctaaacaa tgttcaatca gctgaccttc
    91861 ggtattagct caaagcactg atttcacaac ctctatttag gcatgtgtct cagcaagcgg
    91921 ttgatagttg atgatctgca tttttcgata aaaatcggcg ggaagcttac tccaccgttt
    91981 taataattga tagaaataac tctcttctgc tttgatttta tactcgctta atatcaatcc
    92041 aaaactattc gttttttttg aagctaattt tttataaata tccaatgctt tggttttatc
    92101 ttcattgaca aagagtgttt ttacttcaat ataagaaccc tcgatatttt gtgtttcttc
    92161 gataaaatag atgtttttta tatattcctt catagtgatt cctccaaaga agtcttttat
    92221 ttgataagtt tactctaccg aagagttgtt aataatccgt gaacaaaaag taattctttt
    92281 tccaatagtt gttaaaggtt ttttattttt tatagtattt tttgttattt aagaaaggaa
    92341 taaggttaaa caaataacag gttgcttttt gggtcttttt ccgttcaaga aactgaacga
    92401 agaaaaggga aagttgtctc ttttgtgata agagaaatca taataaacga atgacttact
    92461 ttccaataaa ttgagaacag tcagaaccag acaaattagt tgcatctgat gaataaagtg
    92521 attattctat gacatgaggt gatacagatg aaattagaag tgacaaaaga agcacaagaa
    92581 gtattgaaga ataaattaga agcagacgat caacttttat tagatatcga aaacggtgac
    92641 ggtccatttg cagaaagcca agtcagttgc caattggata cggcatttcg attgatcatt
    92701 gtcaaaaaag atacacaaac agatctaagt ctttattctg tagtggctga tacaccagtt
    92761 ggaccaatca aaatcaaaga cagtgcaata ctttacatgg atgatccatc cacacttgct
    92821 ttagaaccaa cttataatag tttacaatta aaaggaccaa gcggcttgag aaaaggcaac
    92881 ttgcaagttg ttcgaaaaga ctagagggat aaaatgggaa aattggcgat catcagcgac
    92941 ctacatgtgg acatcaataa attcggcgaa tcagagctga tgcagctttg gcaagttttg
    93001 caaaagcaca agatcactcg gcttcatcta gccggagata cggctaataa agtagaccgt
    93061 tgcctagctg ttgtcgaatt tttcgtcaat aaaggattat ctacgacatt tcatttcggc
    93121 aatcacgagt tggcagatgt gcccaatgaa cagatgatgg accattttcc agacccacat
    93181 tttttaaatg aacggtatat tgctttaaat gagcagaccg ttcttttagg gatgaatggc
    93241 tggtacgact atcagttttc cgaattgaaa gattacgagg caatcgtacg aatgaagcgt
    93301 ttgtattggt atgatcggtt catccagcgt gggaaaacag atctcgaggt caatgcagaa
    93361 atgcttgcag ctgcaaaaaa tttgctagac cagctggaag caaaaaaact tgaggtgatt
    93421 ttatccactc atttcgttcc taaacgggca tttatcatgt atcaatctgc tccttatgag
    93481 cgttggaaca aattaaatgc atttttagga tcagaatcat tcggagaatt acttgaccgt
    93541 tattcaaata tcaaacaagt agtttttggc catacgcatc gtcgctttga agataaatcc
    93601 atcggtcaca cgatttacag ctgcagacct tttggttatt attatgaatg gcagctgacc
    93661 cgagattttg tcttgaaaga acagttgcta gacagctatg atcctatgaa attacgtgtc
    93721 ctcttgcgtg cccatcaccc agcttttcag gcctatcaaa cacaacattt gcaagaggaa
    93781 tttgaacaag cgatgacaat cgttccgtat tgagacagca ttagtaatgt gtaaaagaaa
    93841 ataccttgta aaacataagg ataagtaaga gcagtagatc catttatcgt ggatatgctg
    93901 ctttttttgc ggaaaataat cagcgaatgt ttatttgatc ggatgatttg taactcgcag
    93961 agtactagat gcaagcgtgt attctttttt tgaacgataa tcacaaaaaa aatataatat
    94021 acatttttta tttaattata attaatattg attgaagtct aggcagtcaa gtcacatttt
    94081 gatagatagg agggggactt acgaattatt ttaaaggcta tgacagctgt atttctagat
    94141 tgtgtttgca tcaagttgca ataaaaacta gaaatcaaat cgggctgata atcatttata
    94201 actaatcgat acataggacc aaatataggt ctatgttctt ttatttcata agtatcgtaa
    94261 aacagaattt tgctatggat taatccaatc gactgatatg tttagtaaat aaagaaagag
    94321 gaatgtcaat gatgaaaaaa gaaatcgtat taacagcagc aataatgtta ttcagcatgg
    94381 tagcaagtac gacttttgtt tctgcaactg aagtataccc aaaggaatat aacacagaag
    94441 gaacgattac atttgaagca ggagatgagg gtgtaactcc tcctgttgat ccagagaatc
    94501 ctgatcccaa taaacctgtc gatccatcag atcctccaag ccctggaaca ggaggggcat
    94561 tatcgattga ttatggatca aaattcaaat ttgggactca aaaaatttct actgcggata
    94621 aaacgtatta tgcagctgct gatgtgatga acgatgggtc tagaaaaccg acatatgtcc
    94681 aagtgacaga taggagaagt accttatctg ggtggaaatt aagtgtctct caaccagaac
    94741 agtttaaaac tgccagtggg gatgaactag ttggagcaca gctgaaattt acgaaagggc
    94801 aggctgtttc tttggttgat ccaacatata cgccgcaaac ggtgaattct gaattaacgt
    94861 tgacaccggg aggaaacaat acgttagcaa ttaatgctaa atcaggaaca ggtgtgggga
    94921 cttgggtcta tcgatttggt gcaaatgaaa atgaaaatca agatgcggta cagctttcgg
    94981 tacccggtaa atctgtcaag ttagcgcaac aatattccac taaattagtt tggacattag
    95041 aagatacacc taacaactag tttactgaaa agaactgtta aaaataaata cgtagtcata
    95101 ttgtcttgcg aggtaatcga gcaaaaattg tcaaaggcta ggctaaattt gtttggatac
    95161 gaatagttca tctaggaatt taacgatcag gaggaaaaga tatattgaaa aagaggagga
    95221 tcgtgctcat tttaagcagt ctcttagtga ctaatatctt tccgatcata gggtactctt
    95281 tggaattgaa tcagttggag catggaaata gaagagacga agaaagcaaa ctcacttcga
    95341 cattcgacag tttaatagaa tccaaccaga cacaagaaag cagtgaggat ttaagtattt
    95401 cgagtagtgg agagaccact gacaacgtag aagctgaggg ttcggacaaa caaggactag
    95461 aagatgcacc aatttctgcg gttgagctgc ctgacgatcc caatattatt tctatcgata
    95521 aaatttttca aacacctatc ggtactagca cgtccatatt ggaaaatggg aagttactgc
    95581 agttgaaccc agcagaaagg tctcaaagag gagctatttg gtcaaataag aagattagtt
    95641 tactctcgga ttttacattt aaatcttatt tatacttagg aaatcaacga gatagagcgg
    95701 gagatggcat gacctttaca ttgactggcg atcctagaat ggctagtaat cccagtcaag
    95761 ttattggatc gccaggtatg ggaattggct catattctac tcgttctggc caaccttaca
    95821 ttgcgaatgc gctctcgatt gagtttgata cgtataaaaa tagtggttct tctgatcgaa
    95881 tggatcgaga gataactgct gataatagag ggcatggaca tgtcgctttt gtcacgccta
    95941 aaccaaataa taataattat tctggagagc atactggcgt gacggtagct cctacatttc
    96001 tttctaatgg cacatggcgc ttgttaataa ttaaatggga cgcggagcaa agaagtctta
    96061 cttatgattt ggaaggtgtc ggtcgctctt ctcggattat acctgatctc attaggcagt
    96121 ttggttctac agaagtaaat tgggggttta cttcttcaac aggtactttt tttcaagaaa
    96181 atgctgtcgc tgtcacgcga cttccatcga atgtcacatc aacagctgat gtaaaagtca
    96241 acgatagtga ttatggggcc atcgctgaag ctggaggaaa cgataagata actatcagaa
    96301 atacattgaa ggtcgagggg gattttcttg aatataagaa aatgcagact agtatcgacc
    96361 tcccacaaga attaactatt cccgagcata tcttgatcaa tggaaatcaa attgatgcca
    96421 aagatatctt gataaccgga aatcgagtca cgctaaatct gacagattat tttattgctg
    96481 gagatacgtt aataatcgaa ttggagacaa ctttgcagga agtaattcct gaaaaagtac
    96541 tcactatgca ttttgaatac cttgaaaacg ggaatttgat tgagcaatcg aatgaaataa
    96601 acctaaagat cgcaaagctg aaggagatag atatccatgt ttattataaa gataaagaca
    96661 gtcagattga tattgctgaa atgaaaacaa tcactgggaa aataggagag aagtataaag
    96721 aaactcctat tgatattgaa gggtatgttt ttgactcgga ttcagcgaat acagaaggca
    96781 tcttctccga aaatacagag gatatctatt tttattacag aaaaggcaaa ctttatctct
    96841 caactgctcc tcaatatgtc aattttggaa aacataaaat atccagtaga ccattaagtg
    96901 aattcggaca gttttcaggt gggttgaaaa ttatagatga acgggctttt ggaagttgga
    96961 aattacaatt gaaacaagag aaagttctta caaatggaga ttttgagctt ccagaagctc
    97021 tttcctttgt tacctctgaa aatacaacgg ttatagggac atccgctgtt acgattttcg
    97081 aaagtgatca aaagggtgaa agcgatttgt cctctctttt agattctaca gctcatcgtg
    97141 gaatcagaat agatattcct gtagaaaacc aaagaatcgg aacctttgag gggaaactat
    97201 cttggatact tgcagatgtc ccaggaaatt aacaggaaga gaagaatata acaaaattat
    97261 tgagtttcaa gaagggcaca ggtaccatag gctcttgtgt cccacagaga caagattttg
    97321 ttatagtctt ctcttacata tgattaaagg agcgattttc ttgagaccaa aacaacaatt
    97381 atttataaca ctcctagtta ttttaggggc actttttaac aatatatcag tgtcagctag
    97441 tgcatttaat ttttcagtga cgccgataac ttcagagaat caaatcgaca agagaaaaac
    97501 ttacttcgat cttcaacttg atccggatca ggaagtagaa gtaaaagcgg aattgagaaa
    97561 tgatacagaa aaagaagtga aaatcgatat ttctgtgaat agcgcaacca ccaattcaaa
    97621 tgtgatggtt gaatacggaa aaaacgagat tgaaaaagac atgagcctga tctttgatct
    97681 ggtagattat gtgagctatc cacagatagt gacgctaaaa cctaaaagtg tccaaaccgt
    97741 tgcttttcat gtcaggatgc cgaatgagcg atttgatggt gtattggctg gtgggatcac
    97801 attccaagaa atccagacgg aaaaggacca aacagaaact aaggatcaaa gcttatcgat
    97861 tgagaatgaa tacacctata ttattgcttt gttgatgcaa cagacactga acgaagttgc
    97921 tccgaacctt actcttcatg aagttaaacc agatcaaatc aatgcaagaa atgtcatctt
    97981 agcaaacgtt caaaatgatc aaaaaaccta tatcaatcaa gtagttattg aaacaaaaat
    98041 tacaaaaaaa ggacattctg aagttttata tcaagaggaa aaagaaggat tgcagattgc
    98101 gccgaataca aatttttctt ttccaactgc tttaaatggt caaccgctaa ccccaggtga
    98161 atatcacctt acaatgactg ttttaggaaa tgaaaatgaa aatggaaaat tttcgagaaa
    98221 aaaaggaaat actacgatca actataccaa tcaatgggta tttgaaaaag attttacaat
    98281 agacggaaaa gtagccaagg aattgaacac gacggatgtt acgattaaaa aagacaataa
    98341 ttggatttat atcttagtag cttcactttt cttattgttt attttattat gcatactttt
    98401 gtttgttttg agaaaaaaag aagagaaaga aaagtaaggt taagataaca aatagtaaat
    98461 ttttattatt gtaggatggt tgggctcttt ttataacaat ataaaatata ccattttatt
    98521 aataaaaatg catactagta aatgatataa ttctaataac aggtttgatc gcgagaaagg
    98581 ccaaataaaa cgctctccaa aatgttaaaa aaaagacagg acgatgtata ttatacaaac
    98641 aagaatcgct gacaacagtg actcggatcc aggaaatcag ttttatcccc agctagttgg
    98701 tgaatgcatg gaaagtaata tacgtagaaa gagctgatga ctaatgaatt atgagttttt
    98761 tttaagcaaa gaagatcaca gaagttattc attacttaaa tatttagaag caagctcaaa
    98821 tttatcagag tcaatatcga atgttcaaga agagttgtcg ctatcgacat ttctcttgaa
    98881 aaaaacgatc gataagctga aacatgattt acaaaagttc gatctagaga aaaatttgcg
    98941 agtaaccgtc agtgaattgg atatatgtct ggaaattaat ggaaaatgtt ctagcaaagc
    99001 tctgttatct cgttatataa cggattcttt atctatgaaa atggtattgg ctttctttca
    99061 ggaaaaatat atctcagtag aaaattttgc taaacagcat cacgtgagtt attctgtggc
    99121 ttataaagta ttacaaagac taaaaagaaa tctaaaaaaa tatcaaattt tttttgaaaa
    99181 gagaaagttg a
//
LOCUS       DACDYJ010000002        88004 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDYJ010000002 DACDYJ010000000
VERSION     DACDYJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 88004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.002"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(290..1087)
                     /locus_tag="IWM51_000104"
     CDS             complement(290..1087)
                     /locus_tag="IWM51_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP9969554.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1084..2268)
                     /locus_tag="IWM51_000105"
     CDS             complement(1084..2268)
                     /locus_tag="IWM51_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9969555.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2293..3540)
                     /locus_tag="IWM51_000106"
     CDS             complement(2293..3540)
                     /locus_tag="IWM51_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP9969556.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3755..5500
                     /gene="dnaX"
                     /locus_tag="IWM51_000107"
     CDS             3755..5500
                     /gene="dnaX"
                     /locus_tag="IWM51_000107"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP9969557.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5520..5834
                     /locus_tag="IWM51_000108"
     CDS             5520..5834
                     /locus_tag="IWM51_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP9969558.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5859..6455
                     /gene="recR"
                     /locus_tag="IWM51_000109"
     CDS             5859..6455
                     /gene="recR"
                     /locus_tag="IWM51_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP9969559.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6519..7163
                     /locus_tag="IWM51_000110"
     CDS             6519..7163
                     /locus_tag="IWM51_000110"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP9969560.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7178..7507
                     /locus_tag="IWM51_000111"
     CDS             7178..7507
                     /locus_tag="IWM51_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969561.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7521..8459
                     /gene="holB"
                     /locus_tag="IWM51_000112"
     CDS             7521..8459
                     /gene="holB"
                     /locus_tag="IWM51_000112"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP9969562.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8495..9319
                     /locus_tag="IWM51_000113"
     CDS             8495..9319
                     /locus_tag="IWM51_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP9969563.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9312..9659
                     /locus_tag="IWM51_000114"
     CDS             9312..9659
                     /locus_tag="IWM51_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP9969564.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9728..10600
                     /gene="rsmI"
                     /locus_tag="IWM51_000115"
     CDS             9728..10600
                     /gene="rsmI"
                     /locus_tag="IWM51_000115"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP9969565.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10709..11830
                     /gene="dinB"
                     /locus_tag="IWM51_000116"
     CDS             10709..11830
                     /gene="dinB"
                     /locus_tag="IWM51_000116"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP9969566.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11884..12486)
                     /gene="nrdG"
                     /locus_tag="IWM51_000117"
     CDS             complement(11884..12486)
                     /gene="nrdG"
                     /locus_tag="IWM51_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP9969567.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12801..14990)
                     /gene="nrdD"
                     /locus_tag="IWM51_000118"
     CDS             complement(12801..14990)
                     /gene="nrdD"
                     /locus_tag="IWM51_000118"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP9969568.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15316..16083
                     /locus_tag="IWM51_000119"
     CDS             15316..16083
                     /locus_tag="IWM51_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9969569.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16073..18115
                     /locus_tag="IWM51_000120"
     CDS             16073..18115
                     /locus_tag="IWM51_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9969570.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18396..18545
                     /locus_tag="IWM51_000121"
     CDS             18396..18545
                     /locus_tag="IWM51_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP9969571.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18556..20070
                     /gene="dltA"
                     /locus_tag="IWM51_000122"
     CDS             18556..20070
                     /gene="dltA"
                     /locus_tag="IWM51_000122"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP9969572.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            20067..21272
                     /gene="dltB"
                     /locus_tag="IWM51_000123"
     CDS             20067..21272
                     /gene="dltB"
                     /locus_tag="IWM51_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP9969573.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21317..21550
                     /gene="dltC"
                     /locus_tag="IWM51_000124"
     CDS             21317..21550
                     /gene="dltC"
                     /locus_tag="IWM51_000124"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP9969574.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21553..22821
                     /gene="dltD"
                     /locus_tag="IWM51_000125"
     CDS             21553..22821
                     /gene="dltD"
                     /locus_tag="IWM51_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP9969575.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22962..24044
                     /locus_tag="IWM51_000126"
     CDS             22962..24044
                     /locus_tag="IWM51_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP9969576.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            24044..24847
                     /locus_tag="IWM51_000127"
     CDS             24044..24847
                     /locus_tag="IWM51_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9969577.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24917..25657
                     /locus_tag="IWM51_000128"
     CDS             24917..25657
                     /locus_tag="IWM51_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9969578.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25668..26672
                     /locus_tag="IWM51_000129"
     CDS             25668..26672
                     /locus_tag="IWM51_000129"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP9969579.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26717..28561)
                     /locus_tag="IWM51_000130"
     CDS             complement(26717..28561)
                     /locus_tag="IWM51_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969580.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            28773..29291
                     /locus_tag="IWM51_000131"
     CDS             28773..29291
                     /locus_tag="IWM51_000131"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9969581.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            29419..30102
                     /locus_tag="IWM51_000132"
     CDS             29419..30102
                     /locus_tag="IWM51_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP9969582.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            30411..30484
                     /locus_tag="IWM51_000133"
     tRNA            30411..30484
                     /locus_tag="IWM51_000133"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30445..30447,aa:Arg,seq:tct)
     gene            30643..31002
                     /locus_tag="IWM51_000134"
     CDS             30643..31002
                     /locus_tag="IWM51_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP9969583.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            31125..32756
                     /locus_tag="IWM51_000135"
     CDS             31125..32756
                     /locus_tag="IWM51_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP9969584.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(32818..33459)
                     /locus_tag="IWM51_000136"
     CDS             complement(32818..33459)
                     /locus_tag="IWM51_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9969585.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            33741..34049
                     /gene="rplU"
                     /locus_tag="IWM51_000137"
     CDS             33741..34049
                     /gene="rplU"
                     /locus_tag="IWM51_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP9969586.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            34076..34420
                     /locus_tag="IWM51_000138"
     CDS             34076..34420
                     /locus_tag="IWM51_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP9969587.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            34433..34726
                     /gene="rpmA"
                     /locus_tag="IWM51_000139"
     CDS             34433..34726
                     /gene="rpmA"
                     /locus_tag="IWM51_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP9969588.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            35133..37967
                     /locus_tag="IWM51_000140"
     CDS             35133..37967
                     /locus_tag="IWM51_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9969589.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            38158..38649
                     /locus_tag="IWM51_000141"
     CDS             38158..38649
                     /locus_tag="IWM51_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP9969590.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            38752..39741
                     /gene="manX"
                     /locus_tag="IWM51_000142"
     CDS             38752..39741
                     /gene="manX"
                     /locus_tag="IWM51_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP9969591.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            39774..40577
                     /locus_tag="IWM51_000143"
     CDS             39774..40577
                     /locus_tag="IWM51_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP9969592.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            40598..41512
                     /locus_tag="IWM51_000144"
     CDS             40598..41512
                     /locus_tag="IWM51_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP9969593.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            41595..41969
                     /locus_tag="IWM51_000145"
     CDS             41595..41969
                     /locus_tag="IWM51_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP9969594.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            42108..42353
                     /locus_tag="IWM51_000146"
     CDS             42108..42353
                     /locus_tag="IWM51_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9969595.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            42525..43589
                     /locus_tag="IWM51_000147"
     CDS             42525..43589
                     /locus_tag="IWM51_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9969596.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            43835..44260
                     /locus_tag="IWM51_000148"
     CDS             43835..44260
                     /locus_tag="IWM51_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9969597.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            44253..44702
                     /gene="nusB"
                     /locus_tag="IWM51_000149"
     CDS             44253..44702
                     /gene="nusB"
                     /locus_tag="IWM51_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP9969598.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            44799..45653
                     /locus_tag="IWM51_000150"
     CDS             44799..45653
                     /locus_tag="IWM51_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP9969599.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            45654..46991
                     /locus_tag="IWM51_000151"
     CDS             45654..46991
                     /locus_tag="IWM51_000151"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP9969600.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            46996..47220
                     /locus_tag="IWM51_000152"
     CDS             46996..47220
                     /locus_tag="IWM51_000152"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP9969601.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            47222..48112
                     /locus_tag="IWM51_000153"
     CDS             47222..48112
                     /locus_tag="IWM51_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9969602.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            48121..48939
                     /locus_tag="IWM51_000154"
     CDS             48121..48939
                     /locus_tag="IWM51_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP9969603.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            49046..49495
                     /locus_tag="IWM51_000155"
     CDS             49046..49495
                     /locus_tag="IWM51_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9969604.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            49513..51195
                     /gene="recN"
                     /locus_tag="IWM51_000156"
     CDS             49513..51195
                     /gene="recN"
                     /locus_tag="IWM51_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP9969605.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(51250..51366)
                     /locus_tag="IWM51_000157"
     CDS             complement(51250..51366)
                     /locus_tag="IWM51_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP9969606.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(51439..52395)
                     /locus_tag="IWM51_000158"
     CDS             complement(51439..52395)
                     /locus_tag="IWM51_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9969607.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            52566..52934
                     /locus_tag="IWM51_000159"
     CDS             52566..52934
                     /locus_tag="IWM51_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP9969608.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            53104..53535
                     /gene="mraZ"
                     /locus_tag="IWM51_000160"
     CDS             53104..53535
                     /gene="mraZ"
                     /locus_tag="IWM51_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP9969609.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            53556..54515
                     /gene="rsmH"
                     /locus_tag="IWM51_000161"
     CDS             53556..54515
                     /gene="rsmH"
                     /locus_tag="IWM51_000161"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP9969610.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            54520..54921
                     /gene="ftsL"
                     /locus_tag="IWM51_000162"
     CDS             54520..54921
                     /gene="ftsL"
                     /locus_tag="IWM51_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP9969611.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            54922..57114
                     /locus_tag="IWM51_000163"
     CDS             54922..57114
                     /locus_tag="IWM51_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9969612.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            57140..58102
                     /locus_tag="IWM51_000164"
     CDS             57140..58102
                     /locus_tag="IWM51_000164"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP9969613.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            58116..59477
                     /locus_tag="IWM51_000165"
     CDS             58116..59477
                     /locus_tag="IWM51_000165"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP9969614.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            59503..60591
                     /gene="murG"
                     /locus_tag="IWM51_000166"
     CDS             59503..60591
                     /gene="murG"
                     /locus_tag="IWM51_000166"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9969615.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            60606..61826
                     /locus_tag="IWM51_000167"
     CDS             60606..61826
                     /locus_tag="IWM51_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP9969616.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            61982..63307
                     /gene="ftsA"
                     /locus_tag="IWM51_000168"
     CDS             61982..63307
                     /gene="ftsA"
                     /locus_tag="IWM51_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP9969617.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            63330..64571
                     /gene="ftsZ"
                     /locus_tag="IWM51_000169"
     CDS             63330..64571
                     /gene="ftsZ"
                     /locus_tag="IWM51_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP9969618.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            64588..65265
                     /locus_tag="IWM51_000170"
     CDS             64588..65265
                     /locus_tag="IWM51_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP9969619.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            65279..65878
                     /locus_tag="IWM51_000171"
     CDS             65279..65878
                     /locus_tag="IWM51_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP9969620.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            65893..66180
                     /locus_tag="IWM51_000172"
     CDS             65893..66180
                     /locus_tag="IWM51_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP9969621.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            66229..67011
                     /locus_tag="IWM51_000173"
     CDS             66229..67011
                     /locus_tag="IWM51_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP9969622.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            67032..67739
                     /locus_tag="IWM51_000174"
     CDS             67032..67739
                     /locus_tag="IWM51_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP9969623.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            68001..70799
                     /gene="ileS"
                     /locus_tag="IWM51_000175"
     CDS             68001..70799
                     /gene="ileS"
                     /locus_tag="IWM51_000175"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP9969624.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(70848..72374)
                     /locus_tag="IWM51_000176"
     CDS             complement(70848..72374)
                     /locus_tag="IWM51_000176"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP9969625.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(72389..73036)
                     /locus_tag="IWM51_000177"
     CDS             complement(72389..73036)
                     /locus_tag="IWM51_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP9969626.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(73220..73549)
                     /locus_tag="IWM51_000178"
     CDS             complement(73220..73549)
                     /locus_tag="IWM51_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP9969627.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            73726..74454
                     /gene="sfsA"
                     /locus_tag="IWM51_000179"
     CDS             73726..74454
                     /gene="sfsA"
                     /locus_tag="IWM51_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP9969628.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            74470..75483
                     /locus_tag="IWM51_000180"
     CDS             74470..75483
                     /locus_tag="IWM51_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9969629.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            75483..76760
                     /locus_tag="IWM51_000181"
     CDS             75483..76760
                     /locus_tag="IWM51_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9969630.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            76823..79525
                     /locus_tag="IWM51_000182"
     CDS             76823..79525
                     /locus_tag="IWM51_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9969631.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            79677..79994
                     /locus_tag="IWM51_000183"
     CDS             79677..79994
                     /locus_tag="IWM51_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9969632.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            80024..80344
                     /locus_tag="IWM51_000184"
     CDS             80024..80344
                     /locus_tag="IWM51_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9969633.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            80452..81912
                     /locus_tag="IWM51_000185"
     CDS             80452..81912
                     /locus_tag="IWM51_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9969634.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            81980..82201
                     /locus_tag="IWM51_000186"
     CDS             81980..82201
                     /locus_tag="IWM51_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969635.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            82232..82414
                     /locus_tag="IWM51_000187"
     CDS             82232..82414
                     /locus_tag="IWM51_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP9969636.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            82414..82827
                     /locus_tag="IWM51_000188"
     CDS             82414..82827
                     /locus_tag="IWM51_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP9969637.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            82950..84131
                     /locus_tag="IWM51_000189"
     CDS             82950..84131
                     /locus_tag="IWM51_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9969638.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            84520..84593
                     /locus_tag="IWM51_000190"
     tRNA            84520..84593
                     /locus_tag="IWM51_000190"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:84554..84556,aa:Arg,seq:cct)
     gene            complement(84662..85801)
                     /locus_tag="IWM51_000191"
     CDS             complement(84662..85801)
                     /locus_tag="IWM51_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9969639.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(86100..86735)
                     /locus_tag="IWM51_000192"
     CDS             complement(86100..86735)
                     /locus_tag="IWM51_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969640.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(86848..87483)
                     /locus_tag="IWM51_000193"
     CDS             complement(86848..87483)
                     /locus_tag="IWM51_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP9969641.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(87517..87978)
                     /locus_tag="IWM51_000194"
     CDS             complement(87517..87978)
                     /locus_tag="IWM51_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAP9969642.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
BASE COUNT        28520 a        15070 c        18444 g        25970 t
ORIGIN      
        1 ctctgagtca caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac
       61 ttgtgaccga ggagttggta ggagaacacc gtttaccaag aggttgtgaa aaagctgttc
      121 tgcatcaagt aataagaaga gccaagtaaa aacagtctct tatgttttta acttggctgg
      181 acttatcacc gtagatgtaa gcttttgaac gccgtttatt tgcaacaaaa ggggctgtga
      241 cacgattttt gtcacagccc tttttttgcg aacattcatt tagcaatttt tagaaacttc
      301 tgccgccacc gccaaaagtc ccgccaccgc tagaatgggt agtacttccg ccaccgccgc
      361 ttccaggagg tgggcttttg gggattcttc ttgtcgtgac aaaagaattt gtcagacgat
      421 cggttttttc agttaatttg atactggatt tttctcggaa tggatatttg tagatgcctg
      481 atttcaattg ataacgcgaa acagtcacaa aataaaagac aagactaagg ataagagcca
      541 aaacaacagc aatgatgatt tctactgtcg tcaaaacctt atagcgtgta attttgccag
      601 tttcttcgtc tacacgataa tgtcctcctg gaactccgtc ttccacatat gttgctgatt
      661 tagacaggta agcttttgca gcattgtaat aggcagcatt tgtcatctga tcataaacat
      721 catctaagat cgaatcgatt ctgctatcag tcaaataatc gatcatatta ccagaagtcg
      781 aaatgtagat ttcacgctgt cccatatcta atagcaatac tgagccattt tgatcatttc
      841 cgactgcgtc tcttaagtaa ttatcagcaa agtctcttgg ttcttctgaa ttagatgtag
      901 tcgttacgat caagactcgt cctttgattt tttcattcag cttttctgcc tgttgttcca
      961 aagattggat ttgatctgtt gtgaataacc cagcctcatc atttaccgta gctgtatctg
     1021 catgagtagc caccggccag ccaacactta ctagcaaaag aaagaataaa aaaaattttt
     1081 tcatcatatc aagtaacctc cgatcagaaa gaggatcgct agcccagcga aaatcccaag
     1141 agcggtcagt cccaattttt tctggctgat cggcaggaca ccgctgacct ttcctgtttg
     1201 tccattcatt gcgtagtagt agactttttt acttgagtca ttgcttcgat aagtaacaag
     1261 ccataccggc agtaatacgt aattatttac ttcgtttgtg attgaagcag aagttcgcac
     1321 accggtcaat gttgtatatc catttgcggt atcttttaac agcttctctg aatagtcttt
     1381 taattcagat tgaatctgtg atttgattgc ctcatattcg atatctcttt tttctgcttg
     1441 gaacccggct aaatactggc ttttaaaatc aattgcttta tctaacggga aaggttgcac
     1501 gccttcgacc atcttttgtt gcgtattttt tgataaggca ttttttacta gttctttaaa
     1561 agaaagcttg cctgctcttg aaacagcaaa ttgcttcgtt tctgtgtatt cgatgtcacc
     1621 tatccgccat atgcggataa ctgtaccatt cgcctgcatg gcaccatcta cttcagcatc
     1681 tgtagcccaa taagggaaat acacacctgt caacttatcg atttgatcct tattgaaaaa
     1741 agctttggga acaaaccatt ttttcttggt ccaagcgaga aatctgttga ttgcttcttc
     1801 tttctcgaca gcaaaaggaa gtactttatt tggcaagaat tctccgctta aacgtcccga
     1861 tagtacgacg ggattatggc agtagtaaca ataagtcgca gcagttgtcg catcagtcac
     1921 gatctctgca ccacagttgg gacaaagaaa aagatctatc gctgcggtat tatctgcaga
     1981 ttcgtccgct gtatctgaaa aaccgcctgc ctcgtcaacc gctttgcgct cggcttcatt
     2041 catatcggca agctgttctt gggctgtaaa agtaaaatct tcggtaggtg aaggttcttc
     2101 tttgctttct tcgccagcca tacgcgcttc tttttgcttt tgctcatatt ggctgacttc
     2161 ttcttctgta tagatattca ggcagtattc gcaatgaaat ttttggtctt tgggatcaaa
     2221 agtcaacgga ccgccgcaat taggacattt atgtgtaaga acatccatca gctgcacacc
     2281 tttctaaata aattaattaa cggggattcc tttaaacggt ttaccacagt ttggacagaa
     2341 tttagggatg ccgttggaaa gatccacgat ctcattgcat tcactgcatc gcatttgtag
     2401 cttaggctga acgttttcta caggtttggc agctccgcag ttggaacaga attttccgga
     2461 attttctgtc ccgcaaactg gacatgtcca agtatccgca tttccttgtg cagtttgttg
     2521 attttgtttt tctgcttgtt gtttgatttg ttcttggtta ttttgagctg cttgagaaag
     2581 ataagaacca tttgcattca ttcccatacc tacacccata aagccggtca ttgctcctgc
     2641 ttcatttttt ccggctgctt ccataccacg tgcaatggaa ccttgcacgt atccttcacg
     2701 tacgctagga tcacctagca tcgccccttc gttacgcata ttgatcagtt tcacagaatc
     2761 atcggtgtaa gaaatgctgg ctacagccac agaaacaatc tccatcccac gtaattcacg
     2821 ccaagaatca tccaatgcag tatccatgta tttgcttaat tccaaactct tagatggtac
     2881 ataagaaatc cgttgaccat ctgcagacat ctgattgatt gctgattgta gcgctgttag
     2941 gaattcagct aaatactgct cattgatatc attgatttcc acttgtgttt tgtttttagg
     3001 gattgcgttt gtataaaata aaatcgggtc aacgacatga atagaataag tgccatgtgc
     3061 ccgtaagaat aattctgcgt tatagaaatt gtcaaagtag ttcaacggcg cgcttgtacc
     3121 gaatttgatt ccctttattt cttggagatt gatataaaaa acttgttgtt tctgcggtgt
     3181 gacgccgcca aatttgaatc ggtcaaaagt ttcagcaatt gcttctttca gtgttccatt
     3241 gaacatggaa ggagctgcgt catttttaat ggtgtaataa ccttcctcag cggtataatc
     3301 gatgattttt ccaccatcga ccaaaagcat catcatgttt ggatagacat ggacgactgt
     3361 tccgtctgtt aaaacatctt ctgttccttt acgattggag ccacgtttgt catctttgcg
     3421 gactttgaca ccttttgtca tgaccgttgt atcactcata ttgtctggtt cgatgacttc
     3481 tagccattga tctgccagac cgccgccaac catacttgtt gctgctttga taagacccat
     3541 aaaaaattcc tcctctgatt cagctagtgc caaacaatta ttgtttattt attataccat
     3601 aggaagatgt gaaacttctc ggactagaga cggaattgaa atggaaaatt gcgtattgtt
     3661 taaatacctg aaaaagccga gaaggatagt agttttttta cctaatatca cgtataataa
     3721 gagatagtga attttttaaa aaagggggat ccttatggca tatcaagcac tttatcgagt
     3781 ctggcgttcg cagcgtttcg aagacatcgt tggacaaaaa gctgtgacac agacactaaa
     3841 aaatgctatt gtttcccaca aaacctcaca tgcctacttg tttacaggac ccagaggaac
     3901 cggaaagacg agtgcagcaa agattttcgc aaaagcaatc aactgtccga acagtaaaga
     3961 cggtgagcca tgcaacgaat gcgaaatgtg tcgatcaatt actgctggta cgcaagaaga
     4021 tgtgatcgaa atcgatgctg ccagcaataa cggagtcgaa gaaatccgat ttatccgtga
     4081 tcgggcaaat tatgcgccta caaaggcagc gtacaaagtg tacattattg atgaggtaca
     4141 tatgctttca acaggagctt tcaatgcctt gttgaaaaca ttggaagagc caaaagaaag
     4201 tgtcatattc atcttagcaa cgaccgaacc tcacaaaata ccagctacga tcatttcacg
     4261 gactcagcga tttgatttta aacggatcaa tacggcagat attgtcgaac atcttgaatt
     4321 tatcttgaaa gaatcgacta tcagctatga accagctgca ttgaatgtga ttgcccgttc
     4381 tgcagaaggc gggatgcggg atgcattgag tattcttgat caggcaatct cgtttagtga
     4441 aggaacaatt actttagatg atgccatgca agtgacaggt agcttaactg acgaaatgat
     4501 ggacagttac ttgagtgctt gcgtgaaaaa agaagtacca gaagcattgg aaacattgaa
     4561 ccaaattctc gcagccggca aagaatcacg tcgctttcta gaggatatgt tacaatactg
     4621 ccgtgacttg ctgatgtatc agcaagcacc taacctagtt gaggaacaat cgagtacgat
     4681 cagaagtaca tttacagaat tggcacaaac tgtttcagcg gatcagctgt tttcaatgat
     4741 aaagattttg agcgatactc aaaatgaaat tcgttttacc aatagtgccg ctatttattt
     4801 ggaagtcgct acagtcaaac ttgcacagct accgtctgct tctgttcctt cagcagatgt
     4861 agcatttgca gatgatcaga tagtaaaaca gctacaacaa gagattaagg aactaagaaa
     4921 cgaaatcaag gaactacgca gtaatacagg aattgagaat acaaaagtac agccggtaca
     4981 gcaaacaaat cataaaaaac agacaagtac ctatcgtgta ccgacggaac gagtatataa
     5041 agttctgcgg gaagcgacaa gacagcatct gaaccaagta aaagaagtat gggaagactt
     5101 gctgatgagt ctatctgtca ctcagcgagc aatgcttaaa gcaagcgaac cagttgcagc
     5161 tggacctaca ggactagtga ttgcgtttga ttatgagatt gtttgtcaaa gagcagcaaa
     5221 tgacgaagaa ttgcagcttt cggtacacaa caacttaagc cgaatgatca aagattatgc
     5281 gcctgatcct gtttatatta caagagaaag ctggccagaa cttcgaagaa attatttgac
     5341 gcaggcacaa gaagagggaa cagaaaactc ttttgcttct gaagactcag aaggagaaat
     5401 cgagttgctt cctcaagaac aagaagtacc agaagaaaca cttgtagtaa aagcagagga
     5461 actgtttggc tccgaagctg ttacagtggt agatgattaa taaaaaggag tagataatta
     5521 tgatgcgtgg aatgggcaat atgcaaggca tgatgaaaca agtacaaaaa atgcaaaaag
     5581 aaatggcaga agcacaagag ctgctaaata aaacagagtt tacaggtaaa gccaccaatg
     5641 aattggtcaa agcggttttt acaggcgatc gtcgcatgaa agatatccaa atcaatgaag
     5701 aagtcgttga tcctgatgat atcgagatgc ttcaggatct tgtcgtaatg gcagtcaatg
     5761 atgcgatcgc acaaattgat gtggaaactg agaaaaaatt agggaaatac gcaaaaggtt
     5821 tgccaggatt ttagttttaa actgaaggag taatgaatat gcaatatcca gaaccaattg
     5881 cgaaactgat cgacagctac atgaaacttc caggaatcgg tcagaaaaca gcgacaagac
     5941 tcgcctttta tacgatcgat atgaaagatg aagtagtaaa tgaatttgct aaatctttgt
     6001 taagtgtaaa acgcgatttg catttttgta gtatctgcgg aaacatcaca gaagaagatc
     6061 cctgcgaaat atgtagaaat ccttcacgtg atcgaagcgt tatcctagtg gtcgaagagc
     6121 cgaaagatgt aatggcaatg gaaaaaatgc gagaatatca cggactttat catgttctcc
     6181 atggtgtgtt atcaccgatg gaagggacag gtccagaaga catcaatatt tcttctctgt
     6241 tgcagcggct gcatgatgca gatgtaaaag aagtgattat cgcaacaaat gcaacaacag
     6301 agggagaagc aacagctatg tatctctcac gtctgatcaa acccgcaggt atcaaagtga
     6361 ctcgtctagc acatggtctg tctgtaggct ctgatatcga gtatgcagat gaagttactt
     6421 tattgaaagc tgtcgaagga agacgtgagt tataaatttc taattaagtg ttttttcaga
     6481 tacaatataa aagcagcaca agagatgggg gcaacaatgt gaacggacta tttattacaa
     6541 tcgaggggcc tgatggtgca ggaaaaacca gcatcttgaa tgaactttac cctcttttga
     6601 aagaagtagc aaaaacagaa atcattcaaa caagagaacc aggaggcatt ccgattgctg
     6661 aagagatccg agcagtgatc cttgatccaa aaaatgatcg gatggatgaa cgtacggaag
     6721 cactgcttta tgcagcagca agaagacaac atctagtcga aaaggttctg cctgctctgg
     6781 caaaagggaa aatcgtttta tgcgatagat ttgtcgacag ttctcttgct tatcaaggtg
     6841 caggccgacg catcggcgtt accgagattg ctcggttgaa tgagtttgct acagaaggta
     6901 ctacgccgga ttttaccctt tatcttgacg tagattcaga caccggatta agaagaatca
     6961 agaaaaacag acaaaatcaa atcgaccgtt tagattcgga aggtttggaa ttccaccagc
     7021 gtgtacgtca tgcttactta aagctggcag aagagaatcc ggaaagaatc cacaaagttg
     7081 atgcaagaaa aagctttgaa gaagtgctgc aaacaagtta tcatacaatt attgaacagt
     7141 atccgcaatt ttttgaaaac taggaaggtg aacgtttatg aaaattattt tagcgattat
     7201 ccaagacaaa gacagcaacc gtttatcaaa tgaattgatt gatgcaaata tccgtgccac
     7261 aaaactatca tcgacaggcg gcttccttaa ggctggaaac agtacgttta tcgtgggtat
     7321 tgaagatgac cgtgtagaag aagcattgga aatcatcaaa aaaacgtgtg aatcaagaaa
     7381 gcaatttgta tctacacctg ttacactgga tatttcgatg gatgggggcg tcccttatcc
     7441 agtagaagta gaagtaggcg gtgccacagt cttcgtctta cctgttgaag gattccatca
     7501 attttaggag gagctgagct atgaatcaag aaataaactt ggctcaaatg caaccgatcg
     7561 tgtatcagca gcttcaacgg agttttgagc atgggcgtat cgctcatgct tatctttttg
     7621 aaggggagaa aggaacagga aaacatgaag taggtatatg gcttgcacag cacctgttct
     7681 gtacccaaat gaaagacaat ctgccttgtg gaaaatgcaa caattgtcag cggatcgaaa
     7741 agaaagaaca tccagatgtt ttggttattg aaccggaagg acaaaccatc aaagtcgatc
     7801 agatcagacg tttgcagacg gaattcagtc gcagcggtta cgaatcaaga aaaaaagttt
     7861 ttttgatcaa agaagctgaa aaaatgaata gcagtgcggc aaacagtttg ttgaaattct
     7921 tggaagagcc ccctggagat tttttagcgg tgttagaaac agatgcagta ggaagaattc
     7981 tccctactat tcaatctcgt tgtcaagttc tgcattttca agaactttcg aaagaagcac
     8041 tgatccatcg attgcaagaa gagcagattc cattggaaaa agcaaaactt ttggctttct
     8101 taacaaacag tttaggaaaa gcagttgaaa tctctcaaga tgaatggttt aatgatgcta
     8161 aggacttgat ttatcaatgg ttcgtttact tgcaaaagca agatactcaa gcctttatct
     8221 atgtccaaaa aaaattggta aaatcattta aagaaaaaac acaacagttt acaggattat
     8281 ctgtcttgat gttttattat caggaagcac agcgtcaagc agtagcaaac ggcgaatatg
     8341 gcaaagtgaa acagataaac cagataattg aacggatatt gatcgcagag cagaaactac
     8401 gcagcaatgt cagttttcag gctgttgcgg aacagttcgt tttgcatacg attttttagc
     8461 ttgccgcaaa ggatgatgat caaggaggat ggaaatggta gaagtagtag gtgtccgata
     8521 tagagatgca ggtcatatct attattttgc accaggagac tctgaatatg tttatagtga
     8581 caaggtatta gtcgaatctc agcaatcctt acatattgct actgttgtcc ttcctaaaaa
     8641 agagattgat cccaatgacc ttcctgaagc tatccgtccg atcttgcata aagcgtcaca
     8701 agctgaatta gaaaaagaca aaaaaaacaa aaaagatgct tatgatgcat tgaaaatcgc
     8761 aaaagaaaaa gtgcagttgc gtgagttgga aatgaaaatg gtcaaagccg aatacacatt
     8821 tgatcgaaat aaattgattt tttctttcac agcggacggg cgaattgact ttagagaatt
     8881 ggtaaaagat ctggccgctg tatttcgtac tcgtattgag cttcgacaaa ttggtgtccg
     8941 cgatgaagca aagataatag gcggaattgg tccatgtggg cgtccattat gctgttcttc
     9001 ttttttaggc gatttcgtgc ctgtatcaat caaaatggca aaagatcaaa gtttatcttt
     9061 gaatccaaca aaaatttccg gtttatgcgg acgcttgatg tgctgtctgc aatatgagag
     9121 cgaagaatac gagacagcga aaaaagaact ccctgacttt ggaaaagtaa ttgctacacc
     9181 tgatggtaaa gggaaagtcg tagggttgaa tctgcttagc cgtatcgtta gtattcggct
     9241 gactggacgg gaaaatcctg tcgaatatga atgggatgaa ttgaaagaat ttatccaagt
     9301 aggtgagatg aatggataag agatcattat acgacggatt gaatcaatta gaatcagagt
     9361 tgagaaatac attaacagaa cttgttgaaa tgaagcatgc tttgcatgag atcgtagaaa
     9421 aaaatacaac tttggagatg gaaaatcaac gtctgcggga acacctgcgg gaattgaacc
     9481 aagttgcaca agctccagct gaaaatgtga agcaggaact ttctaagtct cgtatgaatc
     9541 ttgaaaaaat ttatgaagaa ggattccatg tctgctacga tttatatgga tcaagaaggg
     9601 acaatgacga gccttgtgcg ttttgtctag aagtgatcta tcgcgaaagc ggtcgatgac
     9661 caataacaag aggagactgg agcggagaaa tactgctcca gtctttttat taaggagatt
     9721 gtgacagatg tataatcaaa aaagtttcaa gggaaatccg gtatatgggg atttatatct
     9781 tgtaccgacg ccaattggaa atttagaaga tatgagctac agaagtgttc gtatgctgca
     9841 agaatccgat cttatcgcaa gtgaggatac gcgtaataca caaaagttat tgaatcattt
     9901 tgaaatccat acaccccaaa aaagtttaca tgaacataat tataaagaac gtgtccctca
     9961 attgattgct ttgttaaaag aaggaaaaac gattgctcaa gtcagtgacg caggaatgcc
    10021 ttccatcagc gatcctggac atgagctagt tttagcctgc ataaaagaag ggatttctgt
    10081 gatctctatc ccaggaccta cagctgggat gacggctttg attgcttcag ggctagtgcc
    10141 tcaaccattt ttattttatg gcttccttcc acgtaagaaa aaagaacaga aagaaacatt
    10201 gaaaaagcta aaagaacaga cagttacgct gattttttat gagtcacctt atcggatcac
    10261 tgctacattg acgaatttct tagaaatttt tggaaatcgc caagtcgttt tgtgtcggga
    10321 attgacaaag gtccatgaag aataccttag aggaagtacg gaagagctac tcgcttatat
    10381 cgaagaacat ccagtaaagg gtgaatgttg tctgcttgta gaagggaatt tgttcgaaat
    10441 gccagtgacg gaagttgatg aagagttggg aacgttaaag gaacaagtag aagcaaagat
    10501 cgcttcaggc gaaaaaccaa atgctgctat caaagcagta gcgctgaaaa atgggctgaa
    10561 aaaacaagaa gtctaccgtc agtaccatga attagactga gatagtataa gttgtctata
    10621 atgaaagact aatgaggaaa tcagctacta acttttcaaa tctcttgaac gcgaacgaat
    10681 gttcttgtat aatgaataag aggtgaggat gatggagctg caatttcctt tacataatga
    10741 tacatcaaga aaaatcattc atattgatat ggatgctttt ttcgcttcgg ttgaagaacg
    10801 agatcatcct gagtttcgcg gacatccatt agtaattgcc agacacccca gtgacactgg
    10861 tgggcgtgga gtagtgacta cagcaaatta tgaagcaaga aaattcggaa ttcattcagc
    10921 aatgagtgca caaaaagcgt atgagctttg tccgcaagcc atctttaagc ctggcgacca
    10981 tcataaatac agcgatattt ccaaacaagt acgggaagta ttctatcgtt atacagatat
    11041 tgtggaacca gttagtatcg atgaagcgta tcttgatgtg accgaaaata aaatcaattg
    11101 caagtcagcc atcaaaatcg cccgaatgat ccaagcagat atttgggaga gtgtccagct
    11161 aacatgttct gcgggagtaa gttacaataa gtttttggcc aaattagcat cagactatca
    11221 aaaacctaga ggaatcacgg tagttgcccc tcaagacgct gtgccatttt taaaagcttt
    11281 gccaattgaa aaatttcatg gggtagggaa gaaaactgta cctagaatgc atgagctagg
    11341 gatttataca ggaaaagacc tatatgaatg cacagaaatg atgttgattc gaaattttgg
    11401 aaaaatgggc tattctcttt atcgtaaagt cagaggaatc catgattctc cagtaaacgt
    11461 tacaagagag cgaaaatctg tgggaaaaga gcatacttat gggcagccgt tgcaaacgga
    11521 agaagcagtg ctgactcagt tgcgccaact agctgaaaaa gtagaagaag cactgagaag
    11581 agtccaaaaa catgggaaaa cagtagtttt gaaggtcagg tacacggact attctactgt
    11641 gaccaaaaga gtgacattgc cagaatatat ctataaaaaa gaagcactct tttatcaagc
    11701 aagccttata tgggaggaaa tcttgggagt agaaaaagga atccgattgt tagggattac
    11761 tttgacgaat ctggacccga tgacttatga aaacatcgtc cttccattat gggaaaatca
    11821 agaaatataa aaacagcttt gagagttgaa accggaaatc cgttttctct cccaaagctg
    11881 tttttatatc aagtcttgct ttttgatttg ttcataggtt tctgttgcat ccgtacattt
    11941 ttcccaaatc acaacttgat tggattctaa agatttttgg acgtcaatga tccgctggtt
    12001 gctacttccg cgaaattgta gttttaggtc acgtttgctt aattcaaagc gtccgtcaac
    12061 aaggatatcg atttgcgaaa ggagttctaa tttatcttct gaatcttgta gcaattcatc
    12121 aaaggtatag cctgtccatg accagatatc ttttgtcgta ccgaactctt ttcggatacg
    12181 tcgtacgact tgcagacaaa catctgtatt caaaaaaggt tcgccaccaa gtaaagttaa
    12241 tccttgcacg taatcgttgc gcaagtcagc gatgatttgt tcttctaatt caggtgtgaa
    12301 tggtttgcca tatcggaaat tttgtgcagc tttgttgaag cagccttcgc aggcaaataa
    12361 gcagccacta acgtaaagac tgttgcggac accttcacca tcgacaaaat taaacggttt
    12421 gtagtcagca atatagtttt ggctgtagtc ttttgctaac cattcttttg gttttggatt
    12481 tctcataaaa aaacttcttt catttaaggt tcaaagatat agctattgaa gataaaatct
    12541 actcgaccac ataaataaga acaaactgac ctaaatccat tgttcaagaa agcttgttct
    12601 tattttgata gagggcgttc aaagatacaa agccatcgaa agagggtggt gaaaaaagac
    12661 ttgtcatagc ctcttattct gaataaacgg tgttcaaaag cttgcatctg cagtaataag
    12721 cccaactaaa cgaaaaatat gaggaactat ttttgtttag ctattcttat tacttgatgc
    12781 aggacagctt tttcacaacc tcatttcatg tgtttcacac gtgaggaaat ttctttatgg
    12841 cggccatgta ccatcggacg agcttgcgga tttcctaaat agccacaagt acgtttgact
    12901 acatcacatg tttttggatc atgatttcca cattctggac attcgaaccc tcgttctgtc
    12961 ggtttgaaat caccttcgaa accacattga tagcaatgat cgattggcgt attcgtacca
    13021 agatacccga ttttgtcata tgcaaaatcc catacagctt ctaaagcttt cggattttgt
    13081 tggagcatag ggtattcaca gtagtggatg aatccacctg aacaatattt tggataatcc
    13141 ttctcaaatt ccaatttttc aaatggcgtt gggtttttac gcacatcata gtggaaactg
    13201 tttgtataat attctttatc tgtgatattt tcgatgatgc cgaatttttc tgtatccaaa
    13261 cggcagaaac ggtcagttag actttcactt ggagttgaat agacactgaa atgataacca
    13321 tattcatccc cccatgcatc tgcattagct tttaattctt tgacgatatc aagtgtgaaa
    13381 gcttttgctt ctgggtttgt ttcccattcg ccaccgaaga aagctgaagc aacttcatac
    13441 aatccaatgt agcctaacga tacagtagct cgacggtttt tgaataattc attgacagaa
    13501 tcggtacgtg aaagacgttt cccgaaagca ccatacatat ataaaattgg cgcatttgct
    13561 ggaatggctt ctttacaacg ttctacacga tagagaagcg catctttcat gatttctaag
    13621 cggtcagaca gtaactgcca gaatttattc tgatctcctt ttgattcgat cgcgattctt
    13681 ggcaaattca atgtaacaac acctaaattc attcgtccga catttacttc ttgaccattt
    13741 tcgtctttcc acccttgcaa gaatgaacga cagcccatag gtactttgaa acttccagtt
    13801 aattcaacga ttttatcgta attcagaata tcaggataca ttcttttcgt tgcacattcc
    13861 aatgctagtt gtttgatgtc gtaattagga tcttcagggt ttaagttcac accttttttc
    13921 aaagtgaaga tcagtttagg gaagatagct gtccgctttt cactccctaa tccattgatt
    13981 cgaatctgta aaattgcttt ttgaatttct ctttcaaacc agttttctcc tagtccaaat
    14041 cctaatgaag tgaaaggagt ttgtccattt gaagtaaata aggtattgat ttcatattcc
    14101 aaactttgca tagcatcgaa gatatctttt ttggtttttg cttttgcata ttctttttgg
    14161 cgttctggcg aatcgatcca ttcttccgcg tcttttagat gttttttata gttcaattct
    14221 gcaaaaggag caagcaattc atctgttcgg tcggcagaac agccaccata ttgacttgac
    14281 gctacattgg caataatctg tgagatttgt gctgtggcgg tctggataga ttttggcgat
    14341 tcgacttcgg cattgccgat tttgaaccca ttgttcagca tgcctttgaa atcaatcaag
    14401 cagcaattcg tcatcggtgt ataaggatga taatccaaat catggtaatg gatatcgcct
    14461 ttttgatgcg catttgctat atgtggtggc aacattttca gtccgattga ttttccaacg
    14521 atcccggctg tcagatctcg ctgtgtattg aaaacattac tgtctttatt tgcgttttcg
    14581 ttcacgacag tttggtcctt gttgattagc ttttcaatcg aaaagttgat atctgttgct
    14641 ttactgcgag caaaatctcg tttggtacga taattgatgt actcttctgc taattcatat
    14701 tcgttttttt ctagtaatat atgttccacg atgttttgaa tctcatagat tttgacattt
    14761 tcagcaaacc ggcttttgat ttcttggaca accaagtctg caatcatttg aattttttca
    14821 taagtaagtg gtgataatga gccgtgaatt ttcttttctg ctttgatcag tgcttcattg
    14881 atcttttgtt catcgaaatc aacatggcgg ccatcgcgtt tgatgacttt catctgggat
    14941 gtctctggct gctgagtaac gacagttttt tcttcataat ttactttcat tatcttcaac
    15001 tcctgctaaa ttgttcttgt ctatcataaa acaaaatcgg ggaaggagca actaaatatc
    15061 ggctatatat agtattcaat gtgctgtata attggatttc tatactatat gtagtgtata
    15121 ttttattgtg aaaaaaggtg aaaacttgta gctggtggat ttgttttttg catatttaat
    15181 tttgctgaat aaaataattg aataaaattt tttattcagt ccaagtaaaa gtgacaaaaa
    15241 cataagagca aattattcgt agatatgcta ctatgcaaat aaatagaaat gaaaaatgaa
    15301 agaggaggag aagaattgga aaagatgatc gaagtgaaac atctaagtaa aagctacggc
    15361 agcaaacttt ctcccgtcaa agccttagat aatatctcat tcacagtaga agaaggcgag
    15421 tttgttggga ttatgggccc aagtggtgca ggaaagacaa cgctgatgaa tatcttgtcc
    15481 acgatcaatt tgcctacgat gggcagtgtc tcgatccaag gaaaagaaat cacaaaaatg
    15541 aaaaacagtg agctcagtga ctttcgtcga aaaaaactag gattcatttt tcaagagttc
    15601 aatttgatcg ataccctaaa tgcaaaagac aatatcctat tgcccctagc agttgaacgg
    15661 atgactaaag aggaaatgga aaagagagta agacatgtag ctcaattgtt gaatatagaa
    15721 gaactgctca aacggtatcc cgatgaactt tctgtcggac agagacaacg aattgcagct
    15781 gctagagcac ttgttgttca gccacaagtg atttttgcag atgaaccaac tggctctttg
    15841 gactcgaagt cggctacaga attgttgaac tacttgaaga ccatgaatca aaaagaaaaa
    15901 gcaacgatac tgttagtcac ccatgatcca tatacagcaa gctactgcga tagaatttta
    15961 tttattaaag atggtgtgat tttttctgaa gttgtcagaa gagggacaag aagagagttc
    16021 tttgagaaag taatagacat gcaggcgacg attggcggag gaggaaaaat gaatgctgta
    16081 taaactagcc gttaaaagtt tttttcggca gtcacgaggc tatcttgttt attttttcag
    16141 tttgacttta tccgtaatga tctattattc cttcagcgca atgacctacg atcaatcttt
    16201 agtcagaaga gcaagtcaag atacttcgat agatggtgga ttgagttttg gtggatttat
    16261 cattactgtt gttttgcttt ttttcgtatt cagtgccaac cgatttttct tgaatagaag
    16321 gcaaaaggaa ataggcatct atcagctttt tggtatgaat aaactgcaga tatcaggtat
    16381 atatgtgtta gaaatcatga taattggatt gttcgcctgt atttctggga tattactcgg
    16441 tattattttt tccaaactct tttcaatgat tttggttcga atgatggata tggatcttac
    16501 tagtccattt tttatctcca ttccatcagt agtagatact ttatttgctt ttttcatcat
    16561 ccttttagca gtatctgtat attcaatatg gaaaatatgg cgctatccaa tgattcgaag
    16621 ttttggagaa aaagaacaag cagatagttc aacaatgcgc ttcaggacaa ggcaccgatt
    16681 actcgcagtc atcgggttac tacttttaac gagcggatat tttggagcta gtcattttag
    16741 ggaaatcact tcatggatga tcagttatca ttatcttgat acattagttt tcttttttcc
    16801 ttttttaatt tttttcgttt gtgtggtcgg tacttatttg tttttctgtt atacatttcg
    16861 gttatttttg atcatattga gtaaatggaa gatttattac tatggtatca attttttgat
    16921 aatgggtaac acgcaagttc atttgctcaa aagttggcgt acaaattctc tgattacagt
    16981 cattctcgga atttctttaa tgacaattgg cggcacggct agtatctcta ctattttatc
    17041 ctaccaggag aaagtttcct ccccgatgga ctatcagttt gatgttgaga cagagaaaaa
    17101 aatcaagccg attctagcag aggaagggca gaagataact aaagaaatca ccttgcatta
    17161 taaagtcatt gggagtatct atagtcaaaa tctcgaagaa cctatgtttc aaactgctaa
    17221 tctgatcacg gaaacacaat atcaagcctt cagaaaagtt aatccagatt tgccgaacat
    17281 ttctttgaaa tcacctggtc atactgtttt gcttgatcaa atgcaaactc tttttcggaa
    17341 tatttcatta tatggatcta gtattggact gccagataat caagaattga agattcaaca
    17401 aatgcttcca agtgtattag gtgacgaaac aatggtatat ctaagtcctg tactgattgt
    17461 agatgatacc gtttttgaaa aagcaaaagg tgtggattat caagtagtca atgtagatgt
    17521 atcaggtgga aataatgaaa aaatagatga acgactgagc aatgacctgt cgataaagtg
    17581 gctcaatgct gtttattatt cttatgatat cgtaaatgga agtctacaag gagaaattag
    17641 cacaaagaaa cttccggatg accaaatgaa ggaagcacaa tttagccaag aaagctctcg
    17701 tttgaatttc tcttccagct attcgaaatt gcgtgcagtt aatcggcgaa tcggtatcaa
    17761 tatttttgtc acactatttg tcgggatgat cgtgattatt gctactggta gtattttgac
    17821 agtccggcaa ttagcagaag cagaggaaga aaaagaaaat tatcacctat tgactaagct
    17881 ggggattcca caaagaagga tcagacggat gatcttcgag caaaacgcac ttaccttctt
    17941 tccacccatg atattaggga tcactcacgc agtatttgca atcaatgtct tcactcagta
    18001 tataaaaact gccgattact ggttggctta tcttgtgagt ggcttattga tccttatcta
    18061 tcttttgctt tatgtcatta cgagtcagtt atattgtcgg attatcgagg aatgatatac
    18121 ggaataagac aagataataa atagaactaa attttgaata ccatttattt ggaataagag
    18181 gttgtgacac gatttttgtc acaacctctt tattttttat attttttaaa agtacgagaa
    18241 attataaaga aaaaagcagt gatttgatgt gctattttta taaaagctat gtgacaaaac
    18301 tgttagtaaa aaaacggttt ctgtaagcta gacacatgtt tctttttgtc aaaattagtt
    18361 acactttgtg tgaggttata tgaggaggga atccaatgaa caaatggcgt tctaatccaa
    18421 aattgaatta ttggggaaaa ttcatcattc gaacaatttt ttatagtgca gtactgctgt
    18481 tcctgattta tctgtatcac tataaaaata ttcaaggagg aacttttatc tataacgaat
    18541 tttaggagga agagtatgga aatcaaaacg attattgaag caatcgatga ttggggaata
    18601 aatgaacctg atcgagttgc ttatcaggcg gatgagacgc atacttttgg tgaattgaaa
    18661 caagcatcag acgcacttgc ttattattta gaaaaaaagg tagaaggcga tggaccaatt
    18721 gtcgtttttg gcaatttgga gtttgaaatg attgtctcat ttctaggggt tgtcaaatct
    18781 ggccatgcct atttgccgat tgaagagcat acacccaagg agcgtatcct atcgatcttt
    18841 cgggtggcta aaccatctat ggtcatcagt atcggggact ggatagaaga tattccttcg
    18901 gttcctgtca taacaaaaaa agaatttcaa gaaatcaaga agataccagt aggattttat
    18961 cccagaaata gcgtaaaagg tacagaaaat ttttatatta tttttacttc tggtactact
    19021 ggagaaccaa aaggcgtaca aatcagtcat gataatctag tcagctttgt tcaatggacg
    19081 atggaagact ttgggatcaa gcctggtatg catttcttag cccaagcgcc attttctttt
    19141 gatttatcgg tattttctat ttatcctgca ttagtttctg gcggaatgct gaagccgtta
    19201 aataaagcag ttgttcaaga ctttcgtcaa ttattcgcga cattgccagc cttgaagttg
    19261 aatgtgtggg tttctactcc atcatttatg gatatttgct tgatggaacc tacattcaat
    19321 ggagaaaatg tgccggaatt ggacatgttt ttattttgcg gagaagaatt gacgaaaaaa
    19381 actgcagaaa gtctacttga tcgattccca aatgcacgga tatataatac ttacggtcca
    19441 acagaagcaa ctgtagctat ttcaagtatt cagattgacc agcatgtact agatagttat
    19501 gaccgactac cgattggcta cgtaaaaaaa gacacgaaag tttctattgt ccaagaaggt
    19561 aaacctgttt caaaaggaga aacgggtgaa atcattattg caggaccaag cgtatcaaaa
    19621 ggttacctga acaatcccga aaagacagct gctgcgttct tttgtcaaaa tgacttagca
    19681 agttaccata caggggatgc aggtagacta gatgaatatg ggcttttatt ttatgaagga
    19741 cgtatggatc agcaggtcaa attacatgga taccggattg aactcgggga tatcgaacat
    19801 tatctgctcc aagacaaccg aatcaaacaa gctgtcgtcg taccaaaata ccaaggaaca
    19861 aaagtacagc agctggttgc ttttgtcgta ttagaaaaca aaacgaaaca gccggatttt
    19921 cagttgacta aatctatcaa agaacaatta ctagaagttg taatggatta tatgattcct
    19981 cagaagttcc attatgtcga tctgctgcct cagacagtta atggaaaaat cgatcgaaaa
    20041 aaattaacag ctgaggtgaa tcctgaatga tgttacctca tatgatccct tatgcagagc
    20101 cgttttattt ttttctttta gccattgcgc ttgtaccgat cgttctatct ttattgatat
    20161 gggaaaaaag attaccagtt tatcaatcag cagtgacact tttcttttta tttgtcagct
    20221 ttggaggtaa ttattggcaa cagggagcgg cgctgattgg gtatgttttg tggcagacga
    20281 tacttgtatg gggctatttt gtttatcggc aaaggaaaaa tcagacagga tggttttttg
    20341 ccgccgttat tttagcgata attccactgt ttttaacgaa agtgactcct ttttttgaac
    20401 atggaaaagc atcgttactt ggttttttag gaatctctta tttaaccttt aaatctgtac
    20461 aaatgatcat ggaaataaga gatggcatga tcaaaacata taagccattt cgttatatcc
    20521 agtttctgct gttctttcca acaatctctt ctggaccaat cgatcgctat aggagatttg
    20581 aaaaagatca gctccatccg cctgttcgtg ataaatatgt tgatcttttg ggaaaaggcg
    20641 tccaaaatat ttttgtcggt tttttatata aattcattat cggttattat tttggtcaag
    20701 tactgcttcc tgtagtggga cgcattgcct tacagcatgg tggtatttca tgggcattag
    20761 taggttatat gtacgtgtac agtatgtatt tgttttttga ttttgctggc tatagcttat
    20821 ttgcagtcgg tacaagctac atgatgggat atcagacacc aatcaatttc aataaacctt
    20881 ttttatcatg gaatatcaaa gaattctgga atcgttggca tatgacgctg tccttttggt
    20941 tccgtgatta tgtatatatg cgattgatgt tcttcatgat gaagaaaaaa ttttttaaaa
    21001 gcaagatcgt aatgtcaaat atcggttatt ttgctttgtt tcttctaatg ggtatatggc
    21061 atggattgac gtggtactac atcgtatatg gactgtatca tgcttgtctg atctgtctga
    21121 cagatgcatg gatccgattt aagaagaagc ataaagagca gttgccttct aacaaattta
    21181 cgcatggctt agccgtattt ttgacatttc aagcagtatg ttttagcttc ctgatttttt
    21241 caggattttt agataaactt ttttttagct aacgaatagt gacatattaa tttatataac
    21301 aaaggagaaa aggaaaatgg aagaacaaat tttatcaatc ttagaagaaa taacaggaac
    21361 agatgaagta agaaaaaatc gtgatattga tctatttgat gaagggttgc ttgattcttt
    21421 agcatctgtt caattattag tcgagctaga cggagagctg gatatccaag tccctgtatc
    21481 tgaattcgag cgagaggatt ggagcacacc gaataaaatc attcaacaag caactgcatt
    21541 gaagggttga gtatgaaaaa gaaacttttt gctattttcg gcccggttgt tttagctgtt
    21601 ggcttactgt ttgtgttttt tacctcctcc tatcaagtag atatctctaa tccagaaacg
    21661 attagacgtg cctcttcttc catgtcagtg aacgtgttga aaggagatgc gataaaaaat
    21721 tatgcattat ctgaaaaaca atatattcct ttttttggct caagtgaatt aagcagaatc
    21781 agtccatttc atccgtctgt tctggcagaa aaataccagc gaaactatcg tccatttttg
    21841 ctgggagcac ccgggacaca atcgttaagc caatatatga tgatgcgttc tgcaggagat
    21901 gctatgaaaa acaagaaagt cgtctttatt atttctcctc agtggttcgt taaaaatggt
    21961 gtaaaaactg attactttaa tacgtattat tctgaacttc aaacatacga ctggttgttt
    22021 tcaatgaaga aagtaacgcc tgctgatcgc tatttagcta gaagattatt aactttctca
    22081 aaagtaaaag agaatgatac gttgacggcg attctgcaaa cgatcaaaaa agggaagcta
    22141 cctttacctg aatctttgaa tcagctgcgc agccaatgga acatgctgaa gagagaggat
    22201 gaagtattca gcaatattgg gttgaaagat cgacagcaaa aaatcgatca tgagtcaaaa
    22261 cgattaccaa aacaatacca agaaacagaa ttatccattt tagcaaatca aattggagaa
    22321 cgagaaacga cgaataatcc attcgggttg aaaaacgatt tttatacgca tcgtatccga
    22381 gcccacgagc cagagctcaa acagtcacaa aaaaattggg attatcgttt ttcgccagaa
    22441 ttttctgatt ttcagctggt cttggatcaa ttagcaaaaa accataacga agtattgttc
    22501 atcatcccac cggttaacga gaaatggagc gattataccg gactatctca agaaatgtta
    22561 caaggatttg ccaaaaagat caaatttcaa ttaaatagcc aaggattcaa tcgaatagct
    22621 gattttgtca atcaagcggg taccaactat tttatggaag acacgatcca tctaggctgg
    22681 aaaggctggt tggctgcaga tcaacagatc agaccgtttt tagaagaaaa tcatattact
    22741 gctagtaagt atcatcttga cgatgctttt tttagcaaat cttggcaaca tcaaatacca
    22801 gataaactac aattaaaata aagaaaacag gcgagtgttg ctcgcttgtt ttctttattt
    22861 ttgaatattc tgaataaaag ataaaaatac caagtttgcc tgaacaaaaa tcataagcaa
    22921 tggtataatc acaatatgaa tatagaaatg ggggaaaaca tttggataca aaagaagaac
    22981 agctacttat tgatttaact tctgcaaaag gagtacctgg aaatgaagaa gaggtaagag
    23041 aagtattcag agaatatgcc aaaccattcg ctgatgatat tttttatgac ggtttaggca
    23101 gtgtgattgc caaacatggt gctggcggag gccccaaggt ctttatttct ggacatatgg
    23161 acgaagttgg ttttatggta acaaaaataa ccgaaaaagg attcttggaa ttccaaactc
    23221 ttggaggttg gtgggggcaa gtcatgcttg ctcagcaagt tgaaatcaaa acacgtgaag
    23281 ggaaaatcgt ccacggtgtg attggttcca aaccaccaca tgttttaaca cctcaagtaa
    23341 gaaataaacc ttatgaaata aaggatatgt tcattgatat tggcgcatca agccaagaag
    23401 aagccaaaga gtggggcata cgtcctggag atatggtcac tccatatatt gaatacaaac
    23461 gaatgaatgg cagtaaatat ttactggcaa aagcatggga taatcgtatc gggacagctg
    23521 tttctctgcg agtattagag aatctatcta aagaagcaca cccaaatgtt ttattcgcag
    23581 gaagcgatgt gcaagaagaa gtaggcttgc gaggtgctcg aacaagtaca caccttgtaa
    23641 atcccgatat tgcttttgct ttagatactg gtacagcagg agacactcca ggaatgacac
    23701 caaaagaagc agattcgatt ttaggaaaag gcccacagat tttgattttt gatgcatcta
    23761 tgatcccgca taaaaaatta ttgaattttg ttattgatgt agcagaggaa atggatattc
    23821 cattccaata tacagtgatt tctggcggag gaactgatgc aggacaaatg catttgacaa
    23881 gagatggtgt tccgtctgta gccattaccg ttcctgttcg ttatctgcat tcccatacat
    23941 cagtgattca tgaagatgat tatttgaata cagtgaaact agtcacagaa ttagtgaaac
    24001 ggcttgatag tgaaacagtt gaaaaaattc ggagttatga ataatgaata atagaatacc
    24061 tatttctgaa tatcggaaaa aagcacgagt ttctttggaa aatcaatggg gaatcaatac
    24121 ttggcttgtt ttcttatcta taatatttac gagcgtcatc cagaactttt ttgacggttt
    24181 cagtcctttt ccggaagaaa gcatgcagca gcacttattg agttttctag tcgataattt
    24241 acttttattc gcattcaact atgcattgtt ttacattgcg ctagtagttg tacggggtgg
    24301 cagaccaaaa agtaaccttt tattttcagt tttccaaaga aagtattttg gttcgatact
    24361 tggcatcaat ttgatcaatt cagtcatcaa ctggctactc aacagtttaa tctttctgcc
    24421 catgttttta atcagcggag tgagtactta tatgcagctg cttttcagtt caaatgcggt
    24481 acctacgggt gtcatgccag aagattttct tgatatcagc tttgttttat cgatgatagg
    24541 gatgattttt gtttcagcac tcatcatgag tattgttggc ggtttattcc aatttgctgc
    24601 atggacaaag ttcgactatc ctgatttgtc ggttattcaa tgtttgaaat atggctggtt
    24661 tttattgaaa gatagaattg gtacgtatat tttgttgcag ctgtctttta tcggctggta
    24721 tttgctagga gcaatcgctt tgttcatcgg tcttttgtgg gtcattgttt atgtgaatgt
    24781 gtcagttgca gaattttatg aacaagcacg gattgaaaaa ggaggtccag acgcctattt
    24841 tacttagcat gtaagcagtt tattcatcaa aaaaataact tttgtggtag gataaatctt
    24901 tgagggggaa tgtacgatga aattaactgt tttaggatgc ttaggagcgt atccttataa
    24961 aaatcaaggg acaacaggtt atttgcttca atctgacggg ttcaatttgt tgatcgatgc
    25021 aggtagtgct acgctgatca aattacaaga acaccttgat ccacttgatc tagatgctgt
    25081 gatccttagc cattatcatc atgatcatat tgctgacctt ggtgtattac agtattactg
    25141 gcaacttcat cctaagagaa atccggagac attgctttca atttatggac acacaaaaga
    25201 ttccattcac tttgaagaat tgacgttgga aaatgtctca aaaggacatc cttattttga
    25261 agctgaagaa ttaaaaattg gtccttttct gattactttt atggaaacaa tccatccagt
    25321 agtatgttac gccatgcgta tcgtagaaga aacaacgggg aaagtattcg tatttacagg
    25381 tgattcaggt tatttggaaa gtttcgctga ttttgcgaaa gatgctgatt tgtttctagc
    25441 agatacctat ctttttgaag gaaatgaaca tcatcatgcc catttcactt caaaagaagc
    25501 tggagaactt gcgaaatcag ctaacgtaaa gaaacttgta cttacacacc tacctcagtt
    25561 tggtgattta gaacaattga aaaatgaagc gcaaaaggca gcaggcaaag ggattgatat
    25621 agaacttgct gcagttgaca aagtgtttaa catttaggag gaagaacatg atttttgtac
    25681 caaatgaaaa ccacgaccca agaatcaatt tagcaattga acaatatttg ctgcaagaaa
    25741 tgcctttaga tgaacctatt ttgctctttt atatcaatga accttcaatc atcatcggca
    25801 gaaaccaaaa tacgcttgaa gaaatcaatc gagattatgt ggaagaaaat ggcattcatg
    25861 ttgtccgtcg cttaagtgga ggcggagctg tctaccatga ttttggaaac ctgaacttta
    25921 gttttatcat gcctgatgat ggggattcat tccgtgactt tgcgaaagta acacagccaa
    25981 tcatccaagc acttcatgaa ttgggtgttg ctggggcgga attgaaaggc cgaaatgatc
    26041 tagtcattga tggcatgaaa ttttctggca acgcgatgta tgctaccaac ggtcggatgt
    26101 ttgctcatgg aacaattatg ttcgactctg atatcaatga agtagtcaat gcattaaaag
    26161 taaaaaaaga caaaatcgaa tcaaaaggaa tcaaatcgat ccgttctcgt gtaacgaata
    26221 tcaaaccatt tcttccagaa gaaaagcaaa aaatgacaac agaagaattt cgtcaagaaa
    26281 tcttgttgaa aattttcggc gtagagtcag tagaagaagt aaaaacgtac gaattgactg
    26341 atgaagattg gaaaaaaatc aatcgaatct ctgaggaata ttatcgaaat tgggattgga
    26401 attacgggaa gtctccagat ttcaatttct ctcgccaaaa acgtttttct atcggttcga
    26461 ttgaagtcca tttgaatgtc tcagaaggaa tgatcaaaga agcaaaaatc tttggcgatt
    26521 tcttcggact aggagatatc tctgacgtgg aaaaaaaatt ggttggacag aaatatgata
    26581 aagcatctct atcaaaaata gtcagccaaa ttgatgtgaa gaaatatttt ggtgcgatag
    26641 aaccagaaga attgcttacc ttactttatt gaaaataaaa aatagcggaa tccacatata
    26701 tggattccgc tattttttat acaggattcg ggtaatttcg agagagttga acagtgatcc
    26761 gatcaatgaa agtatctttt tcctgcgtta gtgttcctgt gtaacgatcc gctacgtctt
    26821 tcatcatcat taagtcaaaa cgttcctgat catctaattc ttctgtgatg cctcgactaa
    26881 ataggtttgt atcgtaatgt gcactatgtt cgcaacaaat gacgaaatgt tcctcttgtt
    26941 cgtgtatcga caaataaatt tttcgataca gtggattttc gatggcatga gtttctctaa
    27001 aggaatgctc taaagcgtgg atcaaaagtt gagccagatc gtcatcgttg ataggaagtt
    27061 cttcgggaag atctgcttgg aaggttatct gaatattccg tttggcagct aacttctggt
    27121 aacgcgctaa gataagattt gtcacctggt ggttgcaaaa agaacacagc atttcgaaat
    27181 tttttgcttc ttctaatatc ttttccacat agtttttagc ttttgtttgg tccgtatgca
    27241 ttaaatcttg cagattctcc atctcttgga caaattccag gcgaagtgta tttacttctt
    27301 ggatatgctg tcgaagctgc tcataatgtt gctccaacaa atcatttttg atttttaaga
    27361 aatctacttg tctccgatct ttgtcggtat ttaagatgtg atcgattaga ttgtagctga
    27421 taataacgat tagaatgata atatataaaa tcgtagacca attgatcgtt gtttgataga
    27481 tacctaatgc cgtttggatg taaatcaagc aatggataat ggctaccaag acgatccaag
    27541 gtgtacaagc tacataaaaa cggttattct tatatgcttc gccaatgcat gcaagtgaag
    27601 tgactaaagt gcttataaca ctagcaatat tagcaatcgg cattaattca ggaagttctg
    27661 caggagtaaa agttgcataa agcagaacgc cgataaaaat caaaagttgg aacgtacaaa
    27721 aaattccgta ccatttttta tagtaaaaca ttcggctcca atagtagcag atgatgaaag
    27781 ctgacgttaa gataataaat aaatgagata aaaaagaatg aacgaatggt tcaaaaagaa
    27841 taccacttaa aatatcttct gatacagctt caaatcctaa tgccaaagca aagcaactta
    27901 agagaataag tgagtattca agacgttttt gtttatacat caagtagatc gtatagaaaa
    27961 aggtccctat agcgataaaa acagcgatta tcatcgtcaa tacttgcggc actgattgtg
    28021 aataaataaa actaatcaat ggtccagttg ttcctagata aactttcggt ggcagtccag
    28081 catagttctg ataaggagat gagactgtaa gggtcaattt ttttccgata taatttgctg
    28141 gtaaatctat cacagccaat gagagtccgg gattgctttc ttcctgataa tctttccgtt
    28201 ggtataaaat cttttcgcct agatatacag taatccattg atggtttcct ttgattagta
    28261 aatttgcttc gtccaacggt ttttcaagtg tcgtttccat aatgaatgtt tcgtctttat
    28321 ccacggtagg aatcgtacga gtgtacttgc tgtcaaactt aaaatcagga tcatcttttg
    28381 tatagaaaac ccacgaatct tttatttctt tcatttgatt gctgtccgca ggagaaaaaa
    28441 taaaataaaa gaaataagca attcctgtta aaaataacaa tgataatata atgattatgc
    28501 tccactttat acgtaaataa aaagattctt ttttattata tatgttatta aaaaaagaca
    28561 taaaggtatt cctttctaaa acttgtaatg ttagggtaaa cgttttttaa actaacgtca
    28621 attatttata ataaaaaatg agataaagat gaggaaatag aattagaaac atataattat
    28681 taaagggaaa catttttaca gctataattt gaaaaaaatt tatcgttatt atatgattaa
    28741 gaaggattga ataaagaaag aagttgaaaa cgatacctga ttacaaaaaa ataactttta
    28801 taagttctat aacgttgatt atatttttaa ttcttattct tctcgtcgtc ttggttcccc
    28861 gaatttttgg tttgactcct tatattattg aggacaaaga aatgagtcca tattatgcac
    28921 aaggaaatct gatatatgta cgaccgaaaa atacaaatga gattttagtc ggagatatta
    28981 ttacatatta tgaaaattca ggaaaaaaaa tctcgacgcg tcgcgtaata gccgttgaaa
    29041 acaaccacga agatttttat acaaaagctg atacaaagac ttatattgag cctggagtag
    29101 taaaaaagag aaatattatc ggtagtccca tctttcagat tccatatatt ggactagtcc
    29161 tttcaaaaac ggcatttgca tggattcagg ggttattctt tacgattgct ttttgcctaa
    29221 caggaatcac agcatggaat acctttgtag agttgaaaaa gaagcggaga cgaacacaga
    29281 aatttttgta agaatgttaa aaacataaat gtattccaga gatccaacga ctcttctgaa
    29341 catgaaacac gagaaaacac tcatgcagct tgtagattta gtcagttgtt cgtataatgt
    29401 gtgaagagga ggagtagtat gcgcaatgaa atgatgcatc gtcctgtagt caaagaagaa
    29461 ctattggatt ttatgcgtac ccagcaaaaa aaattaccgg gtgagctagg caaactggaa
    29521 gaagaagctc atgtagcaga agtaccagtt attccccatg aaacagttgt atttttgaaa
    29581 tttttacttg gacagcttca gcctgaacga atcttggaga tcggagcagc tatcggtttt
    29641 tcatcaagtg tcatggccac gacaatcgga gaaaacgctc atgttacgac aattgatcgt
    29701 ttcgatgtca tgatcgagaa agctaaaaaa aactatgaac gtctaggact gacagataaa
    29761 gtgactttgc ttgaaggtca agcagcagat atcttgccgg agttgtctgg tccgtatgac
    29821 tttattttta tggatagtgc aaaatcaaaa tatattgaat ttttacctga atgcttacgt
    29881 ttgttaagaa aaggtggcgt tttgatggta gatgacatct tccaaggagg aacgatcttg
    29941 ctcccagatg aagagattcc ccgtggcaaa cgagctatcc atcgtaaatt aaatgaattt
    30001 cttcgtgttg tgatggatca tcccgatttg acatcgacaa tcttgccatt aggtgatggt
    30061 gtgatcttaa tgacaaagga atcagaaaca atcgagttgt gatacatgat tatggcagcc
    30121 tgtagataat cgattgaatc gagggctcaa ttcgaacttg atgtaaaagt ttctatattt
    30181 gtaagtcaat taaaggatac aggaaatcta aataaacagg agtagcatga agtcaatgta
    30241 gctcctgttt tcaattgata accttagaac agctgaagga gaatgtctcg ttttaaaaga
    30301 ttgatttagt agttgggcca aaataattca ttatttgcta attaaataac tatttcgtat
    30361 tgacagaagc aatttaatta tctataatta ttgatgttcc gctgattctt gtcatagtag
    30421 ctcagctgga tagagcatcc gccttctaag cggacggtcg ggggttcgaa tccctcctgt
    30481 gacgctaaaa aaccgttaga aactgaggtt tctaacggtt tttctttgtt ttttgtgcca
    30541 atattgtttg ttttcaaagg caaataaaat aaaaaaacat ccttatcgag tacaattata
    30601 aggagcaaaa aaataaaact catcagaaaa gaggaagaag atatgacgat actatctaga
    30661 attttagtga ctctagttgc aattgaattt ttttatatca tgtatatcga aacggtgaga
    30721 actgattctg acacgacaag tagggttttt aaaatgagta aagaagagtt aagcagaaaa
    30781 tctgttcaaa cattgtttaa gaaccaagga gtgtacaatg gactattagg agtgggacta
    30841 ctttatggag cttatctttc ttccgcatct aaagaaatca caagtatgct attaatatct
    30901 attttttttg ttgcattata tggcagtttg actagcgatc gttggatttt actcaaacaa
    30961 ggtgggctag ctctgttggc attgctttct attttcttat aaatatagaa aaaataagaa
    31021 tttatctaac aggaaacttt gtgaaaaata ttactttatc tcatgaaata tgatattcta
    31081 atgaatggta ttttagaaaa tttcatgaaa aaggagttag ataggtgttc aaaaattatg
    31141 tttcctcact aaaaaaagaa ttttcaggtt atgatctttc aagatttcag aaagatcttt
    31201 tggctgggat cacagtagct gctgtagcat tgcctttagc cttggctttt ggggtatcaa
    31261 gcggagctga tgcggccgca ggtttgatta cggctatcat tgccggtatc gttattggcg
    31321 gtttatcagg cggtttttat caaatatcag gtccgacagg tgcgatggca gctatcttga
    31381 tgtctattgc ggcaacaaag ggaatgcaag gtgtattgct tgctactttt ttagcgggtc
    31441 tatttttact tgcagcagga cttttccgat taggaacact gacttctttt atcccagcac
    31501 ccgtaattac ggggtttact tcaggtattg ctatcattat tgcattaggt cagatcgata
    31561 atttatttgg tgttcactcg gaaggtacaa acgttattga aaaactaagt agttatcaaa
    31621 cgataggatt tccgataaac acagcctctt tgatcatggg agggctagtc attttaggga
    31681 tgtttatcta tccaaaaaaa tgggcgaaac gaatgcctag ttcgctgctg gctatcattt
    31741 tggcaacagc agttatgcta ttgttccatt taccagtagc aactgtgggt aagattccac
    31801 aaacattgat cagtagtaat cgtttgaata tgggagattt tagcttttct gccttgcaac
    31861 aagtggcagt tccagctatt agcattgctt tattaggaat gattgaaagc ttgctatgtg
    31921 gtgcttcggc tggccgaatg gcaaataagc ccttagatag caatcaagaa ttaatggcgc
    31981 aaggaattgg aaatctgttg cttcctttct ttggcgggat tcctgctact gcggccatcg
    32041 cccgtacaag cgtggccatc aaatctggtg cacaaactag attagctggt atgatacatg
    32101 cagttgtctt actgctctcc atgcttgttt ttgcgccagt gatgtcacag attccaatgc
    32161 cagcacttgc aggtgttttg attgtgacgg cttggcgtat gaatgaatgg gaaacgatca
    32221 aggagctatt tactaataaa tattggtcag cagttctatt gtttatcctt acgatgattt
    32281 gtacagttat ctttgattta agtattgcta tcgtgatcgg ggtgatcggc ggctgtgtat
    32341 tttttgttgt caaaagcgct gctatcacga tttcattaga aacgatcgat tggcaaagaa
    32401 tggggctgcc agaaacggaa aagctgaata actggacagt agtgtatatt agtggtccac
    32461 tgttttttat gtcttcagaa aaactcaaaa ataaattgga agaactaaat catcacgatg
    32521 gtgtcatttt ctccatgcga ggcgtaccaa gtattgattt gacggcacgt gcagtttttg
    32581 atgaatttca agaaaaagca gagcaaagag gacaaacgat tatctatact tccttgcagc
    32641 cagaagtaga aaaacaactt tctcacttgt gggaacatac attcacgcat caacatcaaa
    32701 cagttgctca tgcgttgaca gctttacatg cgaaatatac tgcaattagc cattaaaaca
    32761 atgaaaaaga acagacaaga ctgtaaactg aagattttat ggtcttgtct gttcttttta
    32821 atattgctgt aaaagtgaag ctagtacttg ccagtcttga tggataacag gtacaaggga
    32881 gcgattgtag aaaatagctg ctggatgaaa aagtggaaga attcgatagt ttttttctga
    32941 ccattcgtag gcatcctttt gttctgttaa ttgtaagaca ggccctgtat agagttgacc
    33001 atgatttttg gtaaccgtat attctttgcc aagtaaacgt tgaagaccaa tgtttccaag
    33061 tgtcgcaatt aattttggct ggaccttcat cagttcgtaa tccaatattg gtgcatgcgc
    33121 aaatacttcg gaacgagtag gtgtccgatt tggataaaca gtttctgact gttgtgtccg
    33181 tttattgatc cgatgagtca cgcgatacgg gcggctcctt acagcactgg tgatatacac
    33241 ctcttctcgt gttaagccag ttgaagaaag tgcctgcatc aattcttttc cagcttgacc
    33301 gctaaaagga acatgcgtat caatctctgt tttccctggg gcttctccca cgagcatcag
    33361 tttaggatga agaggtcctt gcccagcgat aaatccttct acaggacggc cgttcattct
    33421 taaagaagct tcttgtatca gttcttttgg gtattccatt tatgctcctc ctttttcttt
    33481 tattataaga ataaaagaaa cacaatgcga ctattttatt tggtagaact gtgaggatag
    33541 aaaagcagaa gtattgcttg aatttttgtt ttactttttg attttaaatt tagttgacta
    33601 acggttgctg ttctggtaag ctagtactgt tgtaattgtg gactccacag ctacaaccgc
    33661 tcagaacaag taataagcac agcatatgct gtcacttcgg atggcgagtc taagtatatc
    33721 agaaggaggt gctttatagc atgtacgcaa ttattaaaac tggtggtaaa caagtaaaag
    33781 ttgaagtggg acaagcaatc tatgttgaaa aacttgacgt tgaagcagga gaaaaagttg
    33841 tttttgacga agttatctta gtaggcggag aatctacaaa agtaggcgct ccaactgttg
    33901 caggtgcaac tgttgaaggt actgttgaaa aacacggcaa acaaaagaaa gtcgttactt
    33961 tcaaatacaa acctaaaaaa cattcacacc gtaaacaagg tcatcgtcaa ccatatacaa
    34021 aagtggttat cgacgcgatc aacgcttaat agcgtttgag aaagaaggcc tatatatgat
    34081 caaaggaaca tttaagcgaa atgacgcagg tcagatcgtt tcattcactt tgaccgggca
    34141 tgctgatgct ggtccttatg gaagtgatat cgtttgtgcg ggtgtttctg cgttagcgat
    34201 cagcactgtc aacgggattg cctcattagc cggttttgag ccgattgttg aaatgaatga
    34261 agaagaaggc ggttatcttt ataccgaagt cacttcagga atgacacaag aacaaaacaa
    34321 tattgctgag attctattag aaaatctttt attaggatta caatcgatag aagctgaaaa
    34381 ctcagaatat atccaaatta aaacaattaa tgataaatag gaggtgcaga ccatgttgtt
    34441 atctatgaat ctacaattat ttgcccacaa aaaaggtggc ggttctacat ctaacggccg
    34501 tgactcagaa tcaaaacgtc taggtgctaa acgtgctgat ggacaaacag ttacaggtgg
    34561 atcaattctt taccgtcaac gcggtacaaa aatctatcca ggtgtaaacg tgggtatcgg
    34621 tggagacgac actttatttg caaaagttga tggcgtagta cgtttcgaac gtaaaggccg
    34681 taacaaaaaa caagtgtctg tttatccagt agctcaagaa gcttaattga atcttatgat
    34741 taaagaggtt gtgacaagac ttttgtcaca gccttttttg tttataaaaa taaaagttgt
    34801 agtggaggct acgaagttta ctttgtcttt ttacacttaa tatttttttg ccaatttgga
    34861 atcaggatgt tttgataact atcgttttca attgatggaa gctatattgt ttgctatcag
    34921 tgaaataatt atatggtaaa cagtgttctt tttcttgttt ccattataaa aatcgtatcg
    34981 ggatttttcg ttcttttaaa ttgaacattt ctatctatcc gcgtgttatc aacaaaacaa
    35041 aatcaggtaa atagttgacg aaacagaaat aaatacatag tattatgttt tgaagcctaa
    35101 gtacaatagt ctatagaaaa ggagcgatac cattggcaaa acgcattgac cgaatttata
    35161 tgtatgtcaa agaaaaaacc gctcatttaa caaaggcaga atatgatcaa ggagtaacaa
    35221 cacaggaaat tgctgaagtg ttagggattc agcgaacaaa tagcagtaaa gatctgaatc
    35281 agctagtgcg cgaaggaaaa ctgataaaga cagacggacg accagtacgt tacatatatc
    35341 aaacactcgt tacgcacgta aagccagtta caaaacatgt agtcagttac aaagaaacgc
    35401 cggtctctga agaaaaaaat acactccaaa tagatactaa ggatatcttt gcaaaaatca
    35461 ttggagctaa cggcagcatg aaaaattcag tggaacaggc aaaagctgcg attttgtatc
    35521 cgccaagagg attaaattgt ttgattacag gacctaccgg atcaggaaag acttattttg
    35581 cacacgcgat gtatcatttt gcaaaagcgc atcatgtaat agatgaggaa acggaactga
    35641 ttgttttcaa ttgtgctgat tatgccaata accctgagct tttgatgagt catttatttg
    35701 ggtacgcaaa aggtgctttt acaggcgcag atgaagaaaa aatggggatt atcgatcaag
    35761 cggatggcgg tatgctgttt ttggatgaga ttcaccggtt gccgcctgaa ggtcaagaaa
    35821 tgatctttta ctttatggat catggaactt acagtcgtct tggggaaaca acgaaatccc
    35881 atgaagcaaa tgtacggatc gtaggtgcga ccacagaaga cccaggttct tctctattag
    35941 aaacttttgt tcggcgtatc ccaatcaata tcaaacttcc ggcttttgaa aaacgtcctg
    36001 caaatgagaa agtcgattta gtaaaaatca tgattgccca cgaagcaaac cgcacgcagc
    36061 gaaagatttc tttgacagaa gatgtagcaa aagcattgat cggcagtgtg acctatggaa
    36121 atataggaca attgaagtca aatatccagt tagtatgtgc acgaggattt ctaaatcata
    36181 tgcaatcacc cgaaatctcg atcacaatcg atgatttgac agaagggatt cgtagcggat
    36241 tgattcaatt agcaagcaac cgcagtgcaa tggcagagct ttctaaaata ttggagccaa
    36301 agataactgt agcaccaaat gatacgatca tgaaaatcca atcggactcc tatgagttac
    36361 cttataatct atatgatatc atcggagata aagcagcttt actgaagtcg gatggtttgg
    36421 atcaagaagc aatcaatcat ttcatttcta cagatatcaa catccatctg aagtctttct
    36481 ataaagacca tggtttttct tttaatgcag acagcaaatt agcagaattt gtagatccga
    36541 aaatcataga agtgaccaat cagatttatg taatggtcaa aagatcttta ccatatgagt
    36601 ttcagcaaaa ctttatttat gctatgagct tgcatatcag ttcttttttg aaaaaaatca
    36661 acttaggaga agaacggcat acgaatgata acatccgtga gatggctttt gattttccag
    36721 atgaatatgc tgttgcgaaa gaagtgcgta gttatatcga aacgtatttt caagtggaga
    36781 ttccagaaag tgaagactat tacttgaccg ttctactcgt ctcgttacgg gcaaatcaag
    36841 caagcggcag aatcggtgta gtcgttgcag ctcacggaaa cagcacagca agcagtatgg
    36901 tccaagttgt ccaacagttg ttagatgcag agaacgtacg agcagtagac atgcctttgg
    36961 atatggatcc gaaaacagca ttggaccgaa ttgaacgcaa tgtacaagaa gtagatgaag
    37021 gaagcggggt catactctta gtagatatgg ggtcccttgc gtcgtttaat tcgcaaattc
    37081 aaagagacac tggaattcca gtacgtactg ttgatatggt aacaacttct ctagttttgg
    37141 aaactgtacg aaaggtctca gtattaggga cagatctaga tatgttgcat gaatcattga
    37201 aaaattttcg aggttacgca gaaatatctt ctgtagaatc tgttcaggaa gcaaaaccta
    37261 ttcataagaa aaaagctgtt ttggctatct gcgcatctgg cgaaggtaca gcacaaagaa
    37321 tcaaagaatt gatcgaacga gcggtaaaca agcgtcaaga aacagaatta gaagttctag
    37381 ctttgtcgat tgtggaagtg aaagaagaac tgccagttat ccaaaaagaa tatcagatca
    37441 tagctactac tgggattact gatttgaata tccaagcccc atttattcct ttagaacgtt
    37501 ttattgatca aaatatcgaa gtcattttgg atcagttgct gatggaatca gaattggagg
    37561 aaactgaatt catctcccta gatgaagagt cagctaaaaa tacgtgtgtt gagtttatct
    37621 ctgataactt catctttatt aatggtagta agttaatcga tccaatgtgg caattttcta
    37681 cacaaatctc tcagacgacg ggaatcggcg atgaagaata tggattcaaa atcaacctag
    37741 tgatgcacac agcaggaatg atcgaacgta ttattcggaa tgaaccattg actgtggaag
    37801 aaaacgaatt gacaaataca acgaatgatc ctctttatag ccaattggca gcctcagttg
    37861 ttctgttgga agatcagata aaagtaaaag tgccgataga agaaatgtat tatctgctac
    37921 gtctagtcca taatcagtta gataaaaaag aatacactgt gccatgaata cacaaaaacg
    37981 atacactttt atagtgtatc gtttttgtgt attggttaag aatcctttaa attcaattag
    38041 tgtatcgtaa aaaagttggc acgctttttg cttatagtag tgtgtgctag gaggtggttg
    38101 ttcacacaga aatgataggg gtttctgtga agtagtgagg aagaaggagt tttgcagatg
    38161 gatattcgat tggcacgtat agatgatcgt ttaatccacg ggcaagtcgc aacagtttgg
    38221 tcgaagcaaa caggtgttga acgtatcatt gtgatcagcg atgtagtcgc tcaagataaa
    38281 ttgagaaagt ttctactaaa agaagctgca ccgccgggga tcacagcaaa tgtaataacc
    38341 gtgcagaaaa tgattcaaat ctatcatagt ggattattat cacgagcaaa agtgatgctt
    38401 ttattcacca atccccaaga tgttgaagcg gttgtccgct gcggtgtaca gttgaagtcg
    38461 ttgaatatcg gcggtatgag ttttactgaa ggcaaatcaa tgattaccaa ttttgtttca
    38521 gtaaatcaac aagatattga ttcctttcgt tacttgaaca gtcaagaagt ggagttagag
    38581 attcgaaaag tacctgctga caggaaaatc cttttgatgg aactactagc gaaaatcaaa
    38641 aaggtgtagc aagaatggtt gttggaaaag gtcaaataat tctgttgtaa tggttagcaa
    38701 ttattaatat ttttgaattt gttacaacaa aagtaaatag gaggtataaa tatggtaggg
    38761 attatcctag caagtcatgg acaatttgct gaaggaatct tacaatccgg ttcaatgatc
    38821 tttggcgaac aagaaaacgt caaagccgtg attttgaaac caagtgaagg tccagatgac
    38881 ttgagagcca aattggaaga agcggttgct tcttttgata atcaagacga agtgttattc
    38941 ttagttgatt tatggggcgg aacaccgttc aatcaatcaa acacattatt cgaagagcac
    39001 aaagataaat gggcaatcgt cagcggtttg aatttaccaa tgttgattga agcctacgca
    39061 tcacgctttt caatggaatc tgctcatgaa attgcagcac acatcattga aacagcaaaa
    39121 gacggtgtaa aagtaaaacc ggaagaatta gaaccggcag aagcaccgaa agcagcagtt
    39181 gaagacgcac aacctaaagg agcacttcca gaaggaactg tggtcggaga cggaaaaatc
    39241 aaatatgtat tagcacgtgt tgactcacgt ctgttacatg gacaagttgc gacagcttgg
    39301 acaaaagcag tgcaaccaaa ccgtattatc gttgtttcag atgcagtatc aaaggatgat
    39361 ctgcgtaaga gactaatcga acaagctgct cctccaggtg taaaagcaaa tgttattcct
    39421 attagcaaaa tgatcgaagt agcaaaagac cctcgttttg gaaataccaa agcattgctt
    39481 ttatttgaaa atcctgaaga cgtattgaca gctgttgaag gtggcgtaga catcaaagaa
    39541 ttaaatgttg ggtcaatggc gcattcggtc ggtaaagtcg ttgtaagtaa agtattgtct
    39601 atgggacaag aagacgttga agcgtttgag aagcttgaac aaaaaggtgt gaaattcgat
    39661 gtacgtaaag tacctaatga ttcacgcgat aacatggatg atattttgaa aaaagcaaaa
    39721 gctgaattag caaaagcgta ataaataata ttaaaattta taggaggctt atcatgtcta
    39781 ttatttcaat aattttagtc gtattagttg cctttctagc tggtatggaa ggtattcttg
    39841 atgaatttca atttcatcaa ccactagtgg catgtacatt gatcggttta gttaccggta
    39901 acctcgaagc tggtatcgta cttggcggta cgcttcagat gatcgcttta ggttgggcaa
    39961 atatcggtgc cgctgtagca ccagatgctg cattagcatc agtagcatca gcaattattt
    40021 tagttttagg tggacaaggt gttaaaggtg ttccttctgc gatcgcgatt gccgttcctt
    40081 tggctgttgc agggttgttc ttaacaatga tcgtacgtac aatcgctgtt ccaatcgttc
    40141 atttgatgga tgcggctgct gaagaaggaa acatccgtaa ggttgaaatg tggcacatca
    40201 ttgctgtatg tttacaaggg gttcgtatcg cggtcccagc agcagcgttg ctatttatcc
    40261 cagcacaaac tgtgcaatca ttccttgaat caatgccagc atggttaaca gatggtatgg
    40321 ctatcggtgg gggaatggtc gtagctgtag gttacgcttt ggtaatcaac atgatggcta
    40381 caaaagaagt atggccattc ttcgttattg gtttcgttgt agctgcaatt tctcaattaa
    40441 cattgattgc tctaggtgct ttaggtgttg cattagctct tatttacttg aacctttcta
    40501 aaatgggtgg ttcatcaaat aatggaggcg gaagcaactc tggtgaccct ttaggcgaca
    40561 ttttgaatga ctattaatct tgaaggagga aaataaaatg gcagaagaaa aaatcaaatt
    40621 atcaaaaaaa gatcgtttag ctgttgcgtg gcgctctacc ttcattcaag gttcatggaa
    40681 ctacgaacgt atgcaaaacg gtggctgggc attttctatg attccagcaa tcaaaaaatt
    40741 atacaaaaca aaagaagaac gttctgcagc attaaaacgt cacttggaat tctttaatac
    40801 acacccatac attgcttctc caattttagg ggttacattg gctctggaag aagaacgtgc
    40861 gaatggcgcg cctgttgatg atgtagcgat tcaaggggtt aaagtaggta tgatgggtcc
    40921 tttagctggt gttggtgacc cagtgttctg gtttacagta cgtccaatgc taggtgcact
    40981 aggtgcttca ttagctatgg gaggaaatat ccttggacca atcatcttct tcttaggttg
    41041 gaacattatt cgttgggcat tcatgtggta tacacaagaa tttggctaca aagctggttc
    41101 taaaattact gatgaccttt ctggcggatt gttacaagac gtaacaaaag gtgcttcaat
    41161 tttagggatg ttcgtattag ctgcccttgt acaaagatgg gtatcaatca aattcttacc
    41221 agttgtatca actgtaaaat tagataaagg tgcttacatc gaatgggata aattgccttc
    41281 aggtggtgaa ggaatcaaga gtgcatttga acaagttaat aatggcttag ccctttctcc
    41341 tgaaaaggta acaacattac aagacaactt agaccaattg attcctggtt tagctgcatt
    41401 aggattaaca ttgttatgta tgtggttgct taagaaaaag gtttctccaa tcgttatcat
    41461 ccttggttta ttcgtagtcg gtgttgttgg acacgtcatc ggacttttat aatccataaa
    41521 aatatagctc gggcgttgtc ccgagctttt tttatatagt atactgttca taaacaaata
    41581 tctggaggga gaaaatggtt caatcaatca atacaaaagt agacttggta attgatgcta
    41641 cttcgtttac gggtctgaca gattacgggc aaatcatgat aggtgataat ggttttgagt
    41701 tttaccatgc gcgagataga cgcaaatata tccaaattcc atgggaagaa gtggattatg
    41761 tactagcatc agtcatgcta aagggaaaat ggattcctcg atatgccatt caaacgaaaa
    41821 aaaatggtac atacacattt tcttccaaag aacctaaaaa agtgcttcgt gcgatcagag
    41881 aatatgtaga tgaagataga atggtacaat cattgagttt ctttgatgta gtgaaacgtg
    41941 gtattcgtag tatgtttaaa cgcaagtaaa aaataaagaa aacttctaag aggaggtttt
    42001 ctttattttt ttactttgca gaagaaaata tgaaatagca aaaccaatta tttgtcccct
    42061 ttaaatccca tatgctaaaa ttgattagca aagaaggagg ttatattatg agttggttat
    42121 ggacattgat tgttggagcc gtgatcggag caattgcagg tgcgatcacc agtaaaggaa
    42181 attcgatggg ctgtgtaacg aatatcattg ctggactagt cggttcaagt attggacaag
    42241 cgattttcgg cgaatgggga ccaaaagctg caggtatggc acttattcca tctattttag
    42301 gcgcagtgat tttggtagca gttgtctcat ttttctttgg aaaacgatca taagttttta
    42361 caaaatagta aactaaaagg agttgcagcg agactgtgac tcctttttgg ctttatttat
    42421 ttaaaataaa aagaagtttt ccggaaattt ttatttaact gtcgtgtacg aagaaaagtt
    42481 tgctataatg gaaaaaagcg tgcaaagaaa gagggagttt cattatgatg ctacgtgtag
    42541 aaaaattgcg caaaaaaatg caagaagaaa atttggattc atttttggtt accagtccgt
    42601 acaatcttcg ttacttaacg aactttacag gtacgacagg attagctgtt attactttgg
    42661 aaaaagcatt ttttattact gattttcgtt ataccgagca ggcggcagcg caggctcaag
    42721 ggtttgaaat tattaaaaat gtcggaccta tttttgatga ggtagcaaac ttagtacgca
    42781 aagaagaact gcactcatta gcttttgagg aaacaacagt ctcattttta gaatattcgg
    42841 ttttagaaga aatcatcgat gcggaattag tacctgtttc acagatgatc gaagaactca
    42901 gagaagtgaa agatgaagaa gaaatcgcta ttatcgaaaa agcatgtcat attgcggata
    42961 tggcatatga tcacatcctg aaaatgatcc aaccgggcat gactgagatt gaagtcgcaa
    43021 atcaactaga cttttatatg cgttctcttg gcgcttcagg tgtttctttt gatacgatcg
    43081 ttgctagcgg attgcgttcg gctatgccgc atggtgtagc aagcaaaaaa atcatcgaac
    43141 aaggtgactt gatcacattg gactttggtt gctattacga agggtacgtt tccgatatga
    43201 cacgtacatt cgctattggt gatcctgggg aaaaattaaa agaaatttat caaatagtct
    43261 tagatgctca attagcagtt attgatgctg caaaaccagg aatgactggt gtccaattag
    43321 atgcagttgc tagagattat atttcaaaac atggttacgg ggaagcattt ggacattcta
    43381 caggacatgg gatcggttta gagatccacg aaggaccaaa cgtttcttct cgtgcagaaa
    43441 agcaatttgt accaggaaat gtgatcactg atgaaccagg gatctactta ccaggaatcg
    43501 gtggagtacg tattgaggat gacttgttga tcacagaaga tggaaatcgt gtgttgactc
    43561 gttcaccaaa agaattgatc atcttatagt ttcatcaata gaagaagtaa gcgtaaaaat
    43621 cagtgttcac agactttgtt atagaaaatc actcgattta atgatacact atagaagaac
    43681 ttttaagctg taagagataa taaagttggg tgttttctcc aattcatagc agtttgcagc
    43741 tgatcggcgg cagttttctt accacgtgat cactatgccg catcatagag aattcggatg
    43801 tatctcgtgc ttaggcaaag ggaggaataa ataaatggca gatgaaaaaa atttgatttt
    43861 aaacgcaaac caagatttag gagagatcgt gatcgctcct gaagtcatcg aagtaattat
    43921 cgggattgca gcttctaaag tagaaggcgt atacggaatg cgcggaactt ttgccagcaa
    43981 tgtcactgaa ttattaggac gtgcagctca cggaaaaggt gtttacttag caaacgagga
    44041 agatggcttg aaagtagatc tttactgtta tctagaatat ggtgtttcag ttcctaaagt
    44101 agcaatggaa atgcaggaac gggtcagcca gcaagtcctt ttcatgacgg atatcgattt
    44161 atctgaagtc aatatccatg tagtagctgt tgttcctgaa aaattagaac aaccaacaat
    44221 cgaagaactt tttgaagacg aagaggaaga gaatgaataa agaattgtca agacacgaga
    44281 ttcgggaaat ggcattgcag gctttatttc cattagactt caatgcagac ctaacaaaag
    44341 aagatgcgat tttcaacgca atcgaattag accaccgcga catgatcaac gaagatgaat
    44401 cagagtttgt tcctgtttat cttgatacgc tagttggcgg tgtctgtgca aaaaaagatg
    44461 aattggataa agtgattgaa aaacatttaa aaaacaattg gcacatcaat cggatctcaa
    44521 aaatggattt ggtgatcttg cgtatcgcga tttttgaaat gacttatgtg acagatgtac
    44581 cagcggctgt tgctttaaat gaagcaatcg agttggcaaa aacatttagt gatgaccgtt
    44641 cacgtaaatt cgtcaacggt gttttatcca atgtattgaa agaattagaa gctggggcgt
    44701 aagtcctagc ttttttctat gaatcatcat aaacctgtta taaatcggga aaattctatg
    44761 gtagaatgaa aacacaaaaa atatctggga gtggttagat ggcagaattg attaacggaa
    44821 aagaattagc tgaaaaaatg caggcagaga ttgctgaaaa aatccaaaaa ttgaaagata
    44881 atggtatcca tcccggatta gtcgttttat tggtagggga aaatccagcc agccaaatct
    44941 atgtaagaaa taaagaacgt gtagcaaaag aaatcggaat ccattcattg gtagagcgtt
    45001 atcctgagac gatttctgaa gacgaactac ttgcagaaat tgaaaaatac aatcaagatc
    45061 ctagttttca tggtatattg gtccaattac cattgccaaa gcatatagat gaagaaaaga
    45121 tcctgttagc tatccgccct gaaaaagacg tagacggttt tcatccgatg aatttaggca
    45181 gactccttgc gggtaaaccg gacaagattc cttgtactcc atacggcatc atgaaaatgt
    45241 ttgaagctta ttctatcgat ccggcgggta aacgagcagt agttatcgga cgaagcaata
    45301 tcgtcggtaa accgatggct cagctactct tgatggcaga tgcaactgtt actgttgctc
    45361 attctaaaac cgaaaatctg gctgaactgg cacaagaagc agatatttta gtcgtagcaa
    45421 taggacgtgg acattttgtc acaaaagaat tcgtaaaacc aggtgctgta gtgatagatg
    45481 tagggatgaa tcgtgatgct aacggtaagc tgatcggtga tgtgaaattt gatgaagtgg
    45541 agcctattgc aggctggatc actcctgtgc caaagggtgt agggccaatg actatcacga
    45601 tgctgatgta tcaaaccgta aaatctgccg aagcttttgg agcaggtgaa tagatggcac
    45661 aagaatattt aactgtcact acattaacga agtatttgaa aagaaaattt gatgccgatc
    45721 cttatttaga acgcgtatat ttaacaggcg aaatatcgaa tttccgttta cgtgccaatg
    45781 cccatcaata tttcagctta aaagatgacc atgcaaaaat atctgctatt atgttcaaag
    45841 gagcattcca aaaattacga ttccagccaa aagaaggaat gaaagtcctt gtcgttgggc
    45901 gtatttcatt atatgaagcg ggaggttctt atcaaatcta tgtagagcac atggagcctg
    45961 atggaatagg tgctttatat caagaattgg aagaacgaaa agaaaagctg atgaaggaag
    46021 gcctttttca agggcctaaa aaaatgctgc cacgtttccc aaagcgaatc gcggtcctta
    46081 ccagtccaag cggagcggtt atccgcgata tcatcacgac ggtgaaaaga agatatccaa
    46141 ttgtccaatt ggttttgttc ccaacagttg tacaaggaaa ccaagcagcg gacaatgttg
    46201 tcaggaatat ccagagagta gaagagatgg gcaatttcga tacaatgatc atcggtcgtg
    46261 ggggtggttc gattgaggat ttatggccgt ttaatgaaga gcgggttgct cgggcgattt
    46321 atcaagctaa tacgccggtg atttcatctg taggacatga aacagatacc acgatcgctg
    46381 atttagtagc agatgttcgg gctgccactc caacagcagc tgcagaatta gctgttcctg
    46441 ttttgtcaga agagttgatg cgcattcaag aacggcaagc tagattgcag caggcgtttg
    46501 tacgccagat ccagcgcaaa caagaacggt tcgagcgtgc tcgacgttcc tatgtatttc
    46561 ggcagcctga aagattgtat gaaggtcaga ccatcaaatt agaccagttg aaacagcgat
    46621 tatatcaagc tacacagcaa atttatcatg cgaaagaaaa acaaacgatc gcattaagcc
    46681 accaattgga acaagtggct ccgctttatc gagttaaagc agctcgccaa gaaactgctt
    46741 atctagaaaa acgcttagaa gagaaaatcc aacaatatat gaaacaacag cagcagcgtt
    46801 ttgctcaagc tgttcaatct cttgatttgt tgagtccatt gaaaatcatg ggaagaggct
    46861 acagctatac gacgttaaat gatcaagtag taaaaagtgt caccgagatt catcaagggg
    46921 atttgctgac gatccattat caagatggaa tcgttaaagg acaagtacaa gagatagaca
    46981 aaggagaata aaaatatgga aaatccaact tttgaagagt cactgcaaga attagaaaaa
    47041 attgtcatgc agttagaaca aggagacgtt ccattagaat cagctctaga ttcgtttaaa
    47101 cgaggaatgg aattaagcaa acattgccaa gatacattag ctaaagcaga agagaccttg
    47161 acgaaaatga tgacagaatc aaatgaagaa gttgattttg acggaaacga ggaaagctga
    47221 gatgagatta actgactttt cagctattca tctacctgct gtagaaaaag aaatcttgtc
    47281 ttttttagat gagcatacaa cggatagagg actctatgat gcgatgagtt attcagtgaa
    47341 agcaggcggg aaaagaatac gtcccttatt acttttaacg gccgttgctt cctttgatga
    47401 gcctattgat gttcccgtct atcaagtagc agctgcttta gaaatggtgc atacgtattc
    47461 cttgatccac gatgatttgc ctgcgatgga caatgatgat cttcgtagag gaaaacctac
    47521 caatcataaa gtattcggcg aagcattagc tatattggcc ggagatggtt tgcttacagg
    47581 agcattccag ctaatcagta tggcacactt aggtaattct cctaagcttc tgctgcttca
    47641 gcaattagct gtttgtgcgg gatcacaagg tatggttgct gggcaagctg cagatatcga
    47701 gggcgaatcg aaaaagcttt ctttagaaga attagcattt attcatgaaa gaaagacagg
    47761 gcatttgatt cgttatgcct tgctggctgg tggcatatta gcgaagcaac ctgaagaaat
    47821 cttattgcta cttcaacgat tagccgaaca tttaggttta gcttttcaga taagagatga
    47881 tctactcgat gtgattggta caacgaaaac attagggaaa acagctggta aagatgaacg
    47941 tatggaaaaa aatacttacc cgcgtttatt aggcctagaa aagacacgtg aggcgttgga
    48001 gattgagctg ttatccgcca acaagatcat agacaaatta gaagaggacg ttttttcttt
    48061 tgatggcggg ctgatgcgcc agatgatcaa gcaatttgat gtggagaaat gagggaaaga
    48121 atggaaaaag aacgcgtaga tgtattagcg gtcaaacaag gattatttga aacaagagaa
    48181 caagccaaac gttcagtgat ggctggtttg atttacaacg aaaaaaacga gcggtttgat
    48241 aaaccgggag aaaaaattcc agtgagtagt gaattaagag tcaaaggtaa aaaacttcct
    48301 tatgtttctc gagggggatt aaaattagaa aaagcactca aagcatttga tctatctgtt
    48361 gaaggaaaaa cattgttaga tattggtgca tccactggcg gatttactga tgcagctttg
    48421 caaaatggcg caaaaatgag ttatgcttta gacgttggct acaatcagct agcttggaaa
    48481 ataagacaag atcctagagt cgtcgtaatg gaaagagtga attttcgtta tgcaaaaccc
    48541 gaagacttta ccttaggaat tccagaagtt gctgttatcg atgtttcttt tatttcacta
    48601 aaactgatgc tgcctccatt acatgctatt ttaaaagaag agggagaagt gattgcttta
    48661 atcaaacccc aattcgaagc aggcagagaa gctgtaggga aaaacggaat tgttcgtgac
    48721 cctcagaccc atcaaaaagt tttagaagac attttgtcat ttgcggcaca aggtggatat
    48781 gatgtgctgg aattaagtta ttctccgatc acggggggag aaggaaatat cgagttcttg
    48841 gctcacttga ggaaagtacc cgaaagcgga acaatcaatt cagctattaa tatggcagaa
    48901 gtggtttcaa atgcacacga acagtttgac cataaataaa ttgtaactgt gaaaatcaag
    48961 tgagaagtat gcggattctt ctttattata ccaaggaaat aagctatcat tgattaagaa
    49021 gaatatgcta aggagcgaag acgtaatgag aaaaaaagat cggcatcgtt tgattactcg
    49081 attgttgagt gagcaagata tccgaaagca ggaagaattt gttgaaatcc tgaaaagcaa
    49141 aggaatatct gttactcaag ctacgatttc cagagatata aaggaactga aactgatcaa
    49201 ggtaccggct tcagatggcg gctaccgtta tagcatacca gctgaaacaa acgaagatgt
    49261 aaatactaaa ttggaaaaac tattaaaaga cgctttcgtg gcagttgacc aaatggaaaa
    49321 atttgttatt ttgaaaacat tgccgggaaa tgcttcggca gcagcaaatt tgattgataa
    49381 acgttacaag aaagaattat tttcgattat caatgatgac gataatgttt tgatgattac
    49441 acgaactgaa gaccaggcag ttttattgaa gaaagacttc ctgcattacc tttaagttga
    49501 ggagcgaatg tgatgctaca agaaatcagt attactaact ttgcgattat tccggaattg
    49561 cggctttctt ttcacgaagg aatgaccgcg ctgaccggag agactggtgc gggtaaatcc
    49621 attattatcg atgctttagg tctgttagca ggaggaagag gttcaagcga ttatatacgt
    49681 caaggagcag aaaaatgtgt gcttgaaggg ttgtttgaac taccaaaaca agaaggattc
    49741 tctgaactga tggttgaatt agggatcgaa acagatgagg acaatctgat tgttcgacgg
    49801 gatatgtcct taactggaaa aaatgtttgt cgagtgaatg gtcatattat cacattagct
    49861 aatctgagaa gaatcggcag ttatttagta gatatccaag gacaaaacga acatcaggaa
    49921 ctgctccagc ctgaatcaca tcttgcttta ttggatcgtt ttggagatgt agctttccaa
    49981 cagaagaaaa agagttatca gcaagaatat atctcttatc gtgagctgga aaaaagggta
    50041 cgaaaagtgc agcaaaatga aaaatcctat gttcagagaa tagatatgct ccatttccag
    50101 caagaagaaa tagctgctgc tgatttgcaa gtcggagaag aagagaaatt aaaagaagaa
    50161 agagaaaaac ttagcaatta tcaaaaaatt gctgatggat tagctgcagg ttacggagca
    50221 ctgacgaata atgaacaaaa cagtgtagat ggtgttggct tggccgtttc agaactgcaa
    50281 gggatcgccc atctagatgt ggagtatgaa gcaatttatg aaaatatcca aagtgcttac
    50341 tatttgctgc aagatgcaat cggcgatatg agtcgtcaga ttgatttatt ggagttagat
    50401 gagagtcgtt tagaagaagt tacgcaacgt ttagaattaa tccgacagtt gaaacgtaaa
    50461 tacggcgaat ccattgagtc aatattggct tactatgacg aaatcaccga agagctagct
    50521 tcttctgatt tttcagaagg tcaattggac aaaatgaaag aggaactaga gcaaaaagag
    50581 cttctacttc agcaacaagc cgcagatctt catgaagcgc gtaaaaaaat cgcaaaagaa
    50641 ttggaaaaat ccattctgca tgaattgaaa agtctgtata tggaaaacac tgagtttgag
    50701 gtacgtttct taaaagaaga aaatagacaa ttgaatcatg atggatttga ccaaatcgaa
    50761 ttttacatta cgaccaatcc aggagagcca ctgaagcctt tggtgaaagt cgcttctggt
    50821 ggcgaactgt ctcggatgtt gcttgctttg aaaactattt tttcttctga gcaaggtgtg
    50881 acaagtatta tttttgatga agtggataca ggtgtgagcg gaagagttgc acaggccatc
    50941 gcagataaaa tttctcaaat atcgaaatac tctcaagttt tgtgcatcac tcatctgcct
    51001 caagttgctg cagtcgccga ctatcaatat tatattgtga aagcagtgat tgatggacgt
    51061 acgcaaactt ctgtttcaga gttgaaaaca aaagaaagag aagaagaaat tgcacgtatg
    51121 ctggctggaa gtgagattac aaaactcaca ctcgaacatg caaaagaact attgaaactc
    51181 gcaaaaagga cctgagccac ctcgcagtgg ttcaggtcct tttcagcagc tgatagctat
    51241 attttatttt tacatgacac tcattacagc agtcaataca acagatccaa tcgttaaaat
    51301 cagcattgtc caaatgacaa attttgttac tttagcaaaa gtacttgttt gttttttttc
    51361 acgcattgtt ttcaacctcg tcattaatta gttgattcaa gtgtacaatc ttttttccat
    51421 ttccacaaga gggctttttt ataaaaactt cccttttttt aaataatagg tagttacgat
    51481 acaaataaat aatgttaata gaactagcca tataaaggca tcatcccttt cagcaatagg
    51541 taacttaatg ttcattccat agatactgcc gacgattgtc ggtatagtta aaataatcgt
    51601 taaagaagtt aaaattttca taatattatt cagattattc gatacgattg ctgaaaaagt
    51661 ctgattcatt tgttctacta gttttaattg aatgcgagaa gtagtcattg cctgctctgt
    51721 ttccaccaat acatcacgta aatgattttt caaagcatga ttttcttgga aaagtttatt
    51781 ttgcgctagt tctttcaatg catctaaatt agcagaagtc gcttctctga aataaacgag
    51841 gcttttttgg atatccatga tttgataaag ttgtttgttt tcagtggata cttgaagttt
    51901 ggcttccaaa gtatcgcatt gagattgaag cgtagctaaa taactgttgt aagagagtgt
    51961 gacctgccaa atgaattgaa agaagatatt gagattaggc gaaatatcac tttttggaaa
    52021 ggtctggcta aagactttct ttaaaaacag tggttcatga ttcgtaattg tgataagctt
    52081 ctttttgggc gtcaaaataa tagagagcgg gtatgtatcc acttgaaaat aaccgctagg
    52141 acttgtttta gcaaaaggat attgtaacaa taataacgct ggctttgtca actttgattg
    52201 attgaattct tctgtacggg aattttctgc atcgtccaat atggaagtga tgtaatctga
    52261 cggaatttca aattgatgtg tcagctgatg aatctcttct tgattaggac gttctacaca
    52321 ataccagatc gtttggtcag tttcctttcc ggtattacgc agtttctcat tatctaatgt
    52381 aaaatatttg atcatgattt tcctcctgtt gcctgtttct tttattataa cggataggga
    52441 attggaatac atggaatgtg tttgtaagat atagaatata actgcataaa tttaaataaa
    52501 atgaaacagt tgtgtaaagt atatacagta tacttgaagc aacgataaaa aaggagttta
    52561 cgttcatggg cacattcaca ccacttatct tgttttggta tatttacccg gtaatcgttt
    52621 tgtttggttg ccaatttctt gtaaaattat tttcgttaaa tcgtcgtttt aaaataaaag
    52681 cccctgattt ggcgattcct tttttgtggg gcggtatgca tgcattgtct agaaatacag
    52741 ggacaatttc tatcttaccg tttttcttta tttctgtttt gctaatggga atttgtattg
    52801 ctgttttcca agcatattat tatgaagaaa ttatttatcc gcgttatttc aagatgactt
    52861 ggcgtttggt ttttctgttg acaatcgtct tgtacgctgt cttgatcatt gtgaacatac
    52921 tttcttacct atgaaaaaaa tagaaaagct gtaaaaagtc ttttaaaagg ctttttgcag
    52981 cttgattttt aaaaaaatgt ttatgaataa aaaaagttgt tcccggattt cccagaaaag
    53041 gtggtagaaa gtgggggatt gtggtagact aattttagtt agtggaggaa tggggggctt
    53101 tcaatgttga tgggtgaatt ccaacataat attgatgcaa aaggacgttt aatcgtgccc
    53161 tcaaaacttc gtgaagaact tggcgaaaag tttgttctga cacgcggatt ggatggctgt
    53221 cttttcggct atccgatgag cgaatgggaa aatctggaag ctaaactgaa cgaaatgccg
    53281 ttggctaaaa aagacgcacg tacttttgtg cgattcttct attcggctgc cactgaatgc
    53341 gaacttgata aacaaggacg aatcaatata cccagcacgt taagaaatta tgcagctttg
    53401 acaaaaggtt gcgtagtcat cggtgtatct aaccgaattg aaatttggga tgagacacga
    53461 tggcaagatt tttctgcagc agcagaagag aactttgacg aaattgctga aagtatgatt
    53521 gatttcggac tataagaata ggaagaggct gactaatgac tgaaacattt caacactata
    53581 cagtcatgtt gaaagaaacg gtagatggat tgaacatcaa accggatggc gtttatgtag
    53641 actgtacact cggcggtgcc ggacatagtg aatatttatt aagccaacta agttctaaag
    53701 ggcatctgta tgcgtttgac caagatcaaa aagcaatcga tcacgccaag ctgcgtttag
    53761 cttcttatgt cgaacaaggt atggtcacat ttatcaaagc gaacttcaga gaattagaag
    53821 aacgcttgag tgagcatgta gataaggtag acggcatttt gtatgatcta ggggtttcct
    53881 ctcctcagct agatgaagca gaacgtggat ttagttatca tcaagatgca ccgttggata
    53941 tgcgcatgga tcagtcagct cctttatccg cttatcacgt aattaatgaa tattcctacc
    54001 atgaattagt aaagattttc ttccgctatg gcgaggagaa attttccaaa caaattgctc
    54061 gtgaaatcga acgagtacga aaaacaaaac caatccaaac aactggtgag cttgtcgaaa
    54121 tcattaaaca agtcatccca gctcccgcaa gaagaaaagg cgggcatcct gcaaaaagaa
    54181 tttttcaagc tgtgcggatt gcagtaaatg acgaactcgg tgccgaagag gcctctttag
    54241 aacaggcaat ccggttattg aaagtcaatg gaagaatcag cgtaatcaca ttccattcat
    54301 tggaagacag aattgtcaaa agtatgttta aagaatatag tacggtacaa gatctaccac
    54361 cgggacttcc tgtcatacct gacgaattcc aaccagaact gaaagtcatt actcgtaaac
    54421 caatccttcc ttcagaggaa gagctaacag aaaacaatcg atcacgcagt gcaaagttac
    54481 gtattgcaga aaaaataaga atgaaagagg agtagaatga tggcagaatt aaagaaaatg
    54541 caagattacg aatatgattt acctgaattg gtggatcagc cggaacaaca aatgcctgat
    54601 tcttcttcga atcaagaaat cattcttcca caatcaccgg caaaacgatt aaagcagatt
    54661 tctgctttgg aaaaaattgc tgtggcaagc gtggcgattg ctattgttgc tttatgtgtt
    54721 ttaacagtta tgctgcgcac aaatatcagc ggagctgaaa aagaaatcac gtcaatacaa
    54781 acagaaatca atgataaaaa gcaagaaaaa acgagtttag aacaagaaaa aaacgaactt
    54841 tctcgtactg aacggattaa acaaatcgca gaagaaaaag gattgtctat taatgacgac
    54901 aatttaagga aagtgaagta aatgagtctt aaaaataaaa ttcggcggtt tatggaaaag
    54961 aaaaatctaa atccgatgaa caatcgcaaa aaagtaggta tcattttatt tgctacgagt
    55021 attggattgt tctttttatt tgccgtccgt ttctcgtata tcgttattgg aggtcatgtt
    55081 gctggtacct cattagctga aaaaacaaaa cagttgtacc aaggaagtga agtagtaaaa
    55141 gcaaaaagag gaacgattta tgatcgtaac ggtgtggcgc tcgcagaaga tgcctcctct
    55201 tattcaatta aagcgatact ctcaaaaaca tatacctctg gggataaaaa actgtatgtt
    55261 gaagaaaaga attttgataa aatagcagaa atcctgcata agaatctttc tatagacaaa
    55321 aaagatgcac tgaatatttt agaggacggc gcaaaaaaag agctgtatca agttgaattt
    55381 ggtagctatg ggaaaaatat ctcccaagaa accaagcaaa acatcgaagc ggacatgaaa
    55441 aaagaaggtg tcgctggtct ttattttgtc gaccatcagg ctcggatgta tccaaatggc
    55501 gtcttttctt ctcattttat tggatacgca gttccagaca aagatgagaa tggcttagtt
    55561 ggtaaactag gtttggagtc tgcttacaat gatatcttga gcggtaaaga tggtaagatt
    55621 atttatcaaa aagataattt tcaaaatcct ctgccaggta cagtggcaga agaagaaaaa
    55681 gctgtcgacg gacaagatat ctacacgact ttagacagca gactacaaag ttatttagaa
    55741 acactgatgg atcaagtaaa tgaagaatat cagccagaag aattgacagc agtcctgatg
    55801 aaagcaaaaa ccggagaaat cttggcaatg ggacaacgac ctactttcaa tcctgaaaca
    55861 atggaaggat tgactggaaa agatgctatc tggcgtaatt tcttggtaca agatagttat
    55921 gaaccaggat caaccatgaa agttttcact actgcagctg ctatcgaaga aggagaattc
    55981 aacgagaatg aaaccttcca atctggaaaa atccaagtag aagatgctac gatcaatgac
    56041 catgactttg gtgaaaaagg tgtgctgacc atgcgtcaag ctctttcatg gtcaagtaac
    56101 gtcgggatgg tcatactgga acagcgatta ggcggacgat ggtataacta tttacaaaaa
    56161 ctgggatttg gacaaagtac tcattctgga ttagatgatg aagtaaacgg tgctttacct
    56221 acattaaata ttgtcgaccg agcaatgagt gcttatggac aagcggtagg tgtgacgaat
    56281 ttccaaatga tgaaagcttt cacttctatt gcgaataacg gtacaatgat tcaacctcga
    56341 tatatcagca aagtggtgga tccccaaact ggagaagagc gaacaactca aacagaagtt
    56401 ctagggcaac ctttttcaaa agaaacaaca gaaaaagtcc gtgaatatat gagagatgtt
    56461 gtcgaaagcg aaaactacgg tattgcttat ggtgtatata gtgtacctgg atacaatgta
    56521 tcggcaaaaa ccggaacagc ccaaatcgct tctgatacag ggggatatca aacaggagat
    56581 acagcctacc tgtattcgat tgtggagatg gttccttctg aagatccaga ctatgtcttg
    56641 tatctaacaa tgaaacaccc taagacatat gatcgaatgg cattggctaa aatcgcaaat
    56701 ccattgatga aacgggcaat ggattttaaa gaaactgaag aagattcaga tacagaaaca
    56761 aaaacagaaa aagtatccgt agccgattat cgtaatcttg aagcggacgt tgcagcagct
    56821 gatgctcaaa aaagtggact gcaacctgta gtgatcggta atggtaaaaa agtacaaaaa
    56881 caatctactg ccaacggtga tcagttgatc tctggagaaa aactgatcct atatactggt
    56941 ggtgataagc tgatgcctga cgtaacagga tggtcaaaag cagatatcat gaagctgggg
    57001 aaaatcctcg gtattgaagt tagttttgat ggagatggtt attgtgtcaa acaagaactt
    57061 gctccatatg aaaaaatcac tgaagacaaa cttagtttta ctttagaaga ataagaagag
    57121 aaataggaga gaaaagagca tggaatggac acaagcattg attcccattg tcagcagttg
    57181 tgcaatgaca attgccgcaa tgccgctatt tattggttat tttcaaatga aaaagcaagg
    57241 tcaggcgatt cgtgaggagg gaccaaagtg gcacaatagc aaagccggaa ctcctacaat
    57301 gggcggctta gtgtttctga tcggctctat tttgacagga atttgggttg gagcttggca
    57361 aaaacagcta actcctactc ttttcatctt gctttttgta ttagcgttat acggagtaat
    57421 cggctttctt gatgatttta taaaaatatt caagaaaaga aacatgggac tgaactcgaa
    57481 gcagaaattg attggacaga ttctaggggg aatcatcttt tatcttgttt accgttctga
    57541 aggttatcca ggtgtattga atttcttcgg actagatcta cctttaggaa tcatttatgg
    57601 tattttcgct ctcttctggc ttgttggatt ttcaaatgca gtaaatctga cagatggcat
    57661 cgatggatta gttgctggct taggaagtat ctcttttgca gcttacggtc taattgcttg
    57721 gcatcagcat cagtatgatg tattaatcat ttgcttaagt gttttaggag gtttactggg
    57781 tttcttcgtc tacaacagaa aacctgccaa gatttttatg ggagatgtcg gttcattagc
    57841 gttgggcgga cttttagcgg caatttctat aatgctaaac caagaatgga cattgctttt
    57901 gattggtttg atctatgtta tggaaacagc aagtgtgatg cttcaagtta cttcctttaa
    57961 gcttacagga aaacgaattt ttaaaatgtc tccaattcat catcactttg aaatgtgcgg
    58021 atggtcggag tggaagattg atacagtttt ttggttgatc agcattgtga cttcgttgat
    58081 cacattatgg atcgtttggt aggagatgac gggaaatgaa aaaaatatca acatatgaaa
    58141 ataagaaagt gcttgtctta ggactcgcaa aaagcggagt aagtgcggcc aaattattac
    58201 atgaattggg cgcacttgtc accgttaatg atggaaaacc ttttgacgaa aatccggaag
    58261 cacaagagct gctttcttta gggataaaag taataacagg tagtcatcca atcgaattgt
    58321 tggatgaaga attttcattg atggtcaaaa atccagggat tccctacagt catcctttag
    58381 tagcaaaagc ccaagaaatg ggtattccgg taattacgga agttgagcta gcttacgaag
    58441 tagcggaatg cccaattatt gggattacag gaacgaacgg aaaaacaacg acaacaacga
    58501 tgacaggact actactgaat gcaggagcag atcaaggaat tgcacgttta gcgggtaata
    58561 ttgggtatcc agcaagtggt gtagcgcaag aagcaaaatc agaagataaa attgtgatgg
    58621 aattgtctag cttccaactc atgggaatca cagatttccg ccctcatata gcggtgatta
    58681 ccaatatcta tgaagcacat attgattatc atggtacaag aaaagaatat gtcaaagcta
    58741 aatggaacct tcaaaaaaac atgacagaaa aagattatct gatccttaac tggaatcagt
    58801 cagaactaca agaattagcg caaagaacaa aagcacgcgt actgcctttc tcgacaaaag
    58861 aagtgttgga agacggtgtg tatgctgacg attactcaat atactataaa aaagaaaaaa
    58921 taatggaaat atctgaactt ggcgtaccag gtaagcataa tgtagaaaat gcactagctg
    58981 ctatttctgt agcaaaattg tatggtatat caaatgaagc tatacgagaa acgttgcatt
    59041 ttttccatgg cgtaccacat cgtacgcaat atgtaggaga aatccaaggc agaaaatttt
    59101 ataatgattc aaaagcaacc aatattttag cgactaaaat ggctctaagt ggatttgaaa
    59161 cgtctaaggt cgtgttattg gctggcggat tagacagagg aaatacattt gatgaattaa
    59221 ttccttcatt aaaaggaatc aaagcaatgg tcgtatttgg ccaaacaaaa gaaaagttga
    59281 tggatgctgg aaaaaaagca ggcatcgaga caatagtaac agccgattct gtagaacaag
    59341 cagttccttt agcattagaa aacagtacag atggtgatgt agttttatta tcaccagcga
    59401 atgccagctg ggatcaatac ccaaattttg aaactagagg aaacagattc atggaagctg
    59461 tgaaccgatt aaaatagaaa aagtagaagc gagcgataaa aaatgaaaat tttggttaca
    59521 ggcggaggaa cagggggaca tatttatcca gctcttgcct ttgtcaatta tgtaaagact
    59581 caagaaccga atgctgaatt catgtatgtg ggagcaaaac gaggtctaga aaacaaaatc
    59641 gtccctgata caggaatgcc tttccatacc ttggagattc aaggatttaa acgaaaaatt
    59701 tccatgcata acatcaagac ggtccgattg tttcttaaga gtatccgtca agcaaaaaaa
    59761 atcctcagtg attttcaacc agatatcgtt atcggtactg gaggatatgt ttccggagcg
    59821 gtagtgtacg ctgcagccaa aatgggtatt ccaacagtta tccatgaaca aaacagtgta
    59881 ccaggtgtta ccaataaatt tttaagccga tatgtggatc ggatcgctct ttcatttaaa
    59941 gatgcggctt ccttttttcc tgaaaacaaa gcagttcttg taggtaatcc tcgagcacaa
    60001 gaagtagcag atactaaaaa atcagatatt ttgaaaacat ttgatttgga tccagaaaag
    60061 aaaacagtct tgatttttgg cggtagtcaa ggagcactta aaatcaatca agcagtatca
    60121 gtttttttaa gtgaatatat tccttcttct taccaagttt tgtatgcttc aggagataga
    60181 tattatgaag agatcctaaa taaaataaat cctgaaatac aaaatattag tgtccagcct
    60241 tatatcaaaa atatggcaga ggtcatggct tccagtgatc tgctggtggg gcgtgctggt
    60301 gcaacttcga tcgcagaatt cacagctctg gggttaccgg cagtgttggt tcccagccca
    60361 tatgttacga atgatcatca gactaaaaat gcaatgagtc ttgttcacgc aggagcagca
    60421 aaaatgattg cagataatga actaacaggt gaatcactaa gtcagacagt caacgaaatc
    60481 atgggtgatg aagaattgca aaagcaaatg tgccgagctt ctaaagaaca aggaatccct
    60541 gatgcttcaa aacgtcttta tgacttagta aaacaaataa tcaaaaaata aaggcggtgg
    60601 tcttagtgag taagaaaaaa gaaccaaaaa aattgtctca tccgtcttct gatgaaaaca
    60661 atttgactcc ttggcaaaag gctaaccgaa aatatctggc agaacaagag atgaaaaacc
    60721 aagagccaaa aaaagaagac ataacattag atacacccct ttcagattct gaagaagaaa
    60781 aaacggttca agaggaacag caagaagaaa gccagtccga gaaaaaaatc gaattttttg
    60841 aagagatcaa gcaaaagaaa aacggtggac catataacgg ttcttttttg aaccggctgc
    60901 ccaatttaaa aagtcaacgg cacaaagtac tatttcgtcg gttagctttg atcatcactg
    60961 tattgggtat accgctgatt tttttgatct attatgtttc tccttatagc aaattacaag
    61021 cagttgcagt atcaggcaat aagacagtca acagccaaga aatcatctct gatacaaaat
    61081 tgtcattagg agaaaatgtt tggggacagt attttcatcg atctacttat atcgatcgcc
    61141 ttaaaaaagc acagccaaga atcgaaacag caaatataca tttcaaaggg atgaatgaat
    61201 tcgatttgga tgtaacagaa tataaagaga ttgctttgat cgcaaaaaac aatcaatact
    61261 acccagtgat tgaaaatgga acagtattag atgaaaaagt agcaaatcct acaaaaaatc
    61321 tccctatctt ggaagagttc aaagactctg caaaaatcaa agaattaaca aagcaataca
    61381 atcagttatc atcagaatta caaaaagcaa tttctgaaat caagtacacg ccaagagcaa
    61441 gcaataaaaa tctgatccaa ttgaatatga atgacggcaa tcaagttatt gtaaatatca
    61501 ataatcttgc gaaccaaatg aaatattatt cgcaagtagc caaagatatg gatgaaaaag
    61561 gtgtgatcga catggaagtt ggtatcttct cgcatcctta cggtagcact caagaaacgg
    61621 aagaatcggg ccaatcggaa gaaacaactg aaagtgaaga aaatgcggat caaacaaacg
    61681 aatcaagtga agaggcagaa acgcaaaaag cacaaacacc tggatcgtct agcacaaacg
    61741 gatcatctga ttcaaatgat tcaagtgaaa caagtcaaat agaagataat ccaagtacaa
    61801 ctccgtcaag tttaaatgaa aattgataaa acaactttaa aaagtaagaa tgtactttct
    61861 aaaaaccagt aatttatggt agaattggaa gcagtgaata tatctattaa taagtatatt
    61921 ttaaaacgga cgctagttgt tcgcttgtat atggaatagt aagtaggagg gaatcccatt
    61981 catggcaaaa acaggaatgt atgtaggcct tgatattgga acgacatctg ttaaagttgt
    62041 cgtggctgaa tatatcgaca gccaaatgaa tattattgga gtaggaaacg caaaatcaga
    62101 agggattaac cgaggcatta tcgttgatat cgataaaacg gtccaagcga tacaacgagc
    62161 agtacgacaa gcagaagaaa aagcaggtat ccagatcaaa ggcgtaagtg tcggtttgcc
    62221 agcaaacatg cttgaagtag aaaattgcca gggaatgatt gcagttaacg gagattcaaa
    62281 ggaaatcacc gatgaagatg tcagaaatgt ggcttcagct gcccttgttc gttcaatacc
    62341 tcctgaacgt caaatcgtgt caatattgcc acaagatttt actgtcgatg ggttcgaggg
    62401 aatcaaagat cctcgtggaa tgatcggcgt aagattagaa atgtacggtc tgttgtttac
    62461 aggacctaaa acgatcatcc ataacatacg taaatgcgtg gaaaatgccg gattaatcgt
    62521 caacgaaatg gtgattactc ctttagcgct gactgaatca attctttctg acggagaaaa
    62581 agattttggt acgatcgtca ttgatatggg tggcggccaa acaacaacgg ctgttatgca
    62641 tgataagcaa ttgaagttta ccaatcttga ccaagaaggt ggcgaatttg tcactaaaga
    62701 tatctctatt gtattaaata cgtcatttaa caatgcagaa gcgttgaaaa taaactacgg
    62761 ggatgcatac ccagaacgga catcaccaga tgaagaattt ccagtagatg tgattggtca
    62821 gtcagaacca gtgaaagtcg atgaacgcta cttgtcagaa attatttcag ctcgaatgga
    62881 acagattttc aacaaagcaa aagatgcatt ggaccaaatc gaagcattag agttgccagg
    62941 tggcgtcgta ttgacaggcg gagctgctag tcttcctggt gttgtggact tggcacaaga
    63001 aatcttcggc gtaaatgtaa aattgtatgt tccaaatcac atggggctga gaaacccggt
    63061 atttacaaat gtaataagta tcgtggatta ttcagcaaac ttgagtgaag tgtatcaatt
    63121 ggcaaaaatt gctgttacgg gtgaaacctc agcagctcgt caaatggtag ttgaacaaga
    63181 aacaacaaac acatatgaga gctatgaagc acctgaagaa acaatctacg atgagccgga
    63241 accaaaagag tccggagaaa atgtaaaaaa taaaatcaag ggtttcttta caaatatttt
    63301 tgattaattc atcctgggag gaataagata tggaattctc aattgataat aatatcaatg
    63361 acggcgcagt aatcaaagtc atcggagtcg gtggtggcgg tggaaatgct gtaaaccgaa
    63421 tgattgaaga aaacgttaaa ggtgttgaat tcattacagc caacacagac gtgcaagcat
    63481 taaaaaattc aaaagctgaa acagttatcc aattaggccc caaatatact cgtggtttag
    63541 gtgctggttc tcagccagaa gttggacaaa aagcggcaga agaaagtgaa caatccttac
    63601 gcgaagcttt agacggtgca gatatgattt tcatcactgc aggaatgggt ggaggaaccg
    63661 gtacaggtgc tgcaccaatc gttgcaggta tcgcgaaaga attgggcgca ttgactgttg
    63721 gtgtcgtgac acgtccattc acttttgaag gaccgaaacg tggccgtttt gctgcagaag
    63781 gtatcgctcg tttgaaagaa aatgtggata cattattaat tatctcaaat aaccgtttat
    63841 tagaagttgt agataaaaag acaccgatgc ttgaagcttt ccgtgaagca gataatgttt
    63901 tacgacaagg tgtacaaggt atctctgatt tgatcacagc gccaggttac gttaaccttg
    63961 actttgctga cgtgaaaaca gtgatggaaa accaaggtac agcattgatg gggatcggtg
    64021 tggctagtgg agaagatcgc gtagtagaag caacgaaaaa agcaatctcc tctccattgc
    64081 ttgaaacatc tattgatgga gctgaacaag tacttcttaa catcacaggc ggtttggata
    64141 tgacattgtt cgaagcacaa gatgcttcag atatcgtagc aaatgcagca actggcgatg
    64201 taaatatcat tctaggtaca tctatcaatg aagaaatggg cgatgaaatc cgtgtgacgg
    64261 tcatcgcaac aggtatcgat gaatcaaaaa aggaacgcaa gtctagccga ccagctagac
    64321 aagcacaaat gcaatcacct gctcaaaaga ctgtattaga tatggatcaa gctaaaccaa
    64381 cttcttcaga agaagaaaac agttttggcg attgggatat cagacgtgaa caaaatgttc
    64441 gtcctagagt tgatgattcc aattttgaca atatcgaaaa aaaagaattt gatacattca
    64501 accgcgaaga aacaaaatct catggcgatg atgaattaaa tacgccaccg tttttccgtc
    64561 gtaaaaaata aaggagagga agagcacatg attgctgaca acttgcagaa aatcaagcaa
    64621 gaaattcagg attcgtgtgc tcttgttcat cgtgatccga aagaagttac attagtagct
    64681 gtaacaaaat ctgttgaatc cgctcaagct gaagaattgg ttagagctgg cgttatgaat
    64741 tgtgcagaaa accgagtaga taaattatta gaaaagaaac aagatttatt tgattttcct
    64801 gaaataaagt ggcatttaat tggtaattta cagcgcagaa aggtaaaatt gattgtaaat
    64861 gaaattgatt actttcatgc gttggacagt ttaagattag cagaagaaat ccagaagcgg
    64921 ttagagaaag aattatcctg ttttattgaa gtaaatgttt ctggtgagga atcaaaacat
    64981 ggaatttcac cagatgagct tttaccgttt gtggaatctt tagctgcctg cacaaaaatc
    65041 aaggttgttg ggttgatgac tatggcgcct aaagaagctt ctgatgagca aatccataaa
    65101 gtgtttggta cgctgaaaga attgcgtgat cttgttcaag caaaaggtta tgcccatgca
    65161 ccatgtagtg aattaagtat ggggatgagt caagactttc aaattgcgat tgaagaaggt
    65221 gcaacattta tccgtgtagg aacagcgcta ttcaaagacg aataaaggag gaacaaaaat
    65281 gccgctttta aacagaggag caattgctaa ttttttcgga ttagctgatg aggaagagta
    65341 cgaataccaa gattatcctg aaacaacaaa aaaggctgta cagccaacta aaaaaaatac
    65401 aacacagaat caatcaactc attctgtatc tgctgcgaat actcatgaaa aacaaaaaac
    65461 ggcgacttca cgtcctgtaa cgcatgaaag tattcagccg gctgaaaaga aaaaagtaat
    65521 tgaactacat caatcaccta caaaaactgt agcgaaagaa agaacagctt caagaacctc
    65581 tacacaacct aaagcaaatc ttgctggaaa aatcactgta atggaaccaa gagcttattc
    65641 tgaagctatg gcgattgcta aacgaatcat tgctggagaa gcaaccttag tgaattttca
    65701 tttagtagaa gaaaaacaag cgagaagaat cgttgatttt ctaacaggaa cagtttatgc
    65761 actagatgga gatatcaaac gtgttggaga tgagattttt ctttgtacac cgactgggat
    65821 ggagattgac agttcaaccg ctcaatcttt tgcagatgcg aatttgtttg atctgtaaga
    65881 ggaggaacta cgatttatac tattgcacta ttacttcatt tacttcaacg ggcagcctat
    65941 ttctatagca ttatcttgat ggtatatgca ttgctctctt ggtttccagg cgggtatcaa
    66001 tcagggctag gacgattatt agctaagatc tgcgaaccgt atatcagttt gttcgatcgt
    66061 ctgcctttga ggattggacc cttggatttc acaattttag gggcaatcct gatcttgaac
    66121 ttagccgtac aaggtttagc acagatcgtt atatggataa ctgtccattt agtttattga
    66181 ttgaagcaac aaaatcttta ttctcagaat agataggagg ggaaaatttt ggatgcaaat
    66241 gtataccagc actttcgggc agatgaacgt acatttatcg attcggtagg cgattggatt
    66301 gaacaggttc aaggacaata tgcaccctat ctgaccgaat ttcttgaccc aagacaatcc
    66361 tatattttag aaacactgat tcgacaaagc agtgatttgc gtttcatgtt ttatggcgga
    66421 tatgaacagg cagaaagaaa aagatgtttg attttccccg attattacga acctgtagag
    66481 gaagattttg aaacagcatt gtatgaaatc cattatccta gcaaatttgc cagcctaagt
    66541 catgggaaaa tccttggaac tttagtggga acggggatca aacgctctta ctttggcgat
    66601 atcatctcgg acggtgatcg ctggcaagta tttattgcaa atgaggtgga tcattttgtt
    66661 gctgcacaag taacaaagat tggaaaggtt gctgtacgct tggaaccaat caaatacaca
    66721 gaaatgatta tgccgaaaga tagctggaca caagaaagag gaacagttag ttccatgcga
    66781 ctagacagtg tgatctctga gatttataat atttccagac agcggtccaa gcagctgatc
    66841 gaagctggta aagtaaaagt gaactggaca gaaaatacaa gacctgattt tgtacttgaa
    66901 ctattagata ttatttcagt cagaggttat ggcagagtcc agatccaaga acttgagggg
    66961 aagacgaaaa aagaaaagta cagggtactg ttcggtgtat tacgtaaata aaataaaaag
    67021 gtggtttact catggcttta actccattag atatccaaaa taagacattc ccgacaaaaa
    67081 tgcggggata taatcaagac gaagtagatg atttcttaga tttagttgtt cgtgactatg
    67141 aggaattgac tcaaagaaat cgagaattag aaaaagcagt caaacattct gaagaaaaac
    67201 ttgagtactt caatgaatta aaagatgctt tgaaccaatc aatcatcgta gctcaagata
    67261 cggctgataa agtaaaaaca agtgcaagca aggaatccga agtgatcgtt acttctgcac
    67321 aaaacaaagc agatgagtta gttgcgaacg ctgaaaaacg tgcacatcaa ttaacaactg
    67381 atgcagaaga aaaagcaaga aaaatcttga cagatgcaac agaaaaagca agacagcttg
    67441 cgacagaaac tgaagatctt aagaaaaaaa cacgagtatt ccatcaacgt atcagcttga
    67501 tgcttgagtc tcaattagaa caagtgaaaa gtccggaatg ggatgaaatt ttacaaccat
    67561 tctccagcta cgtgactgac agccatgaag tgatcaaaga agttttagca aaacagcttg
    67621 acaatgaaaa tgatactgat gtaaactcag acacaggagt catagaagca cctatgacgg
    67681 catttgatac acaggctatt gatatctcga tcgtgcctgg tgaaaatgaa aacgagtaga
    67741 acagaaaagc actttgttat tcttaaagcg agccgatgag agtggaagtt cggtaagaca
    67801 ctgcatggtg aaagatcctc tacttgagac ctgcttttcc gaaattagag taagaagcag
    67861 cggctgacct cgttattggt cacagagagt ttgcactaga tagatgcaac taaatttggg
    67921 tggtaccacg gcatgttcgt cccttgcggc tgaacatgct tttttgtgtc cggaaaacaa
    67981 ataaaggagt gtatggttga atgaaaatga aagaaacctt gcaattagga aaaacaaaat
    68041 tccctatgcg tggtaatttg ccaaaccgag aaggcgagtg gcaaaaagaa tgggaagaaa
    68101 atcgaatcta ccaaaaaaga caagagttga acgaaggaaa acctagcttt gttcttcatg
    68161 atggacctcc atatgccaat ggtaatatcc atttaggaca ttcattgaat aaaatcagta
    68221 aagacattat cattcgttca aaatcaatgt ctggctatcg tgcaccatat gttccaggat
    68281 gggatacaca tggattgccg attgaacaag tattggctaa taaaggcgtg aaacgtaaag
    68341 aaatgtccat ggcagaatac ttggaaaaat gtcgtgaata tgcactttcc caagtagata
    68401 agcaacgagc agatttcaaa cgcctaggag ttgcaggaga ttgggaacat ccttatgtta
    68461 cattagatcc ttcttatgaa gctgcacaag tacgcgtatt tggtaaaatg gctgaaaaag
    68521 gctatattta caaaggattg aaaccaattt actggtctcc gtcaagtgag tcttctttag
    68581 cggaagctga aattgaatat aaagatgtaa aatctccatc tatttttgta gctttcaatg
    68641 tcaaagacgg caaagatatt cttgatgaag atacagcttt tgtcatttgg acgacaacac
    68701 catggacatt gccagcaaac caaggtattg ctgtaaatcc aaaatttacg tatgtattgg
    68761 tggaagccga tggccgtaag tttgtcgtag cgaaagattt gctagaaaca gtccaatcag
    68821 aaatcggttg ggaaaatgta acagttctaa aagaatttgc tggacaagaa atggaatata
    68881 tgactgcaag tcatccattc tatgaacgtg aatcacttgt gattttaggc gatcatgtta
    68941 ctttagacgc tggtactgga ttagtccata ctgctccagg acatggggaa gatgactata
    69001 tcgctggtag caaatataaa ctatctgtag tgtcacctgt agactcaaaa ggattgttta
    69061 cagaagaagc acctggattt gaaggtatct tttatgacaa agcaaatcct atgatcactg
    69121 atctattaaa agaaaaagga gctttgctga agctagattt ctttactcat agttatcctc
    69181 acgattggcg gacgaaaaag cctgtgatct atcgggcaac tccacaatgg tttgcatcta
    69241 tcgataaatt ccgtcagaat attttggatg aagtagaaaa agtagagtgg gttatcccat
    69301 ggggtaaaac acgtttgtat aatatgatcc gtgaccgcgg cgattgggta atttcccgtc
    69361 agcgtgcatg gggagtacca cttccgattt tctatgctga aaatggagaa gcaatcatca
    69421 caccagaaac aatcgaacat gtggcaaaat tatttgctga acatggatca attatctggt
    69481 ttgaaaaaga agcgaaagaa cttcttcctg aaggatttac ccacccaggt tctcctaatg
    69541 gcgaatttac aaaagaaaaa gatatcatgg atgtctggtt tgattctggt tcttctcatg
    69601 aagctgtttt acgtcagcga ccagaattat cattcccagc agatatgtat ttagaaggat
    69661 cagaccaata tcgtggctgg ttcaactcaa gtatcacgac tagtgtggct atcaatggtg
    69721 tagcaccata caaatcagtt atttctcaag gatttgcttt agatggtgaa ggccgtaaga
    69781 tgagtaaatc tttaggaaac atcattgcac cagaaaaagt gatcaaacaa tttggtgcag
    69841 atatccttcg cttatgggta tctagtgtag attatgaagc agatgttcgt gtttctatgg
    69901 atatcttaag tcaagtggca gaagtatatc gtaaaatcag aaatacgatg cgtttcttat
    69961 tagcgaatac agaagatttc gatcctaaaa aagatactgt ttcgtacaat gatttacgtt
    70021 ctgtagacaa atatatgacc gttcgattga accaagtaat caaagaaatc cgagaagaag
    70081 ggtacgacaa atataacttc atgcatattt accgtacagt tatgaacttc ttgacggttg
    70141 atctatcgtc attctacctg gattttgcta aagatgtagt ttatattgaa gcagaagatg
    70201 attatcaacg tcgttgcatg caaactgttt tctatcaaac agctgtagct ttgacaaaat
    70261 tattgactcc tatcattccg catacagcag aagaaatctg gtcattcctg aaagaagaag
    70321 aggagtacgt tcagttaagc gagtttcctg gttatgaaga gtttgcaaac caagaagaat
    70381 tgatggatac ctggactgct ttcatggact tccgtgataa tgtcttaaaa gcattggaag
    70441 aagcaagaaa cagcaaattg attggtaaat ctttggaagc aaaactaact gtttatccaa
    70501 aacaacaagt tcgtgaaatg ttaaaagctt tggatgcaga tattgcacaa ttattgatcg
    70561 tttcaccaga ctactttgaa gttatggcag cagatgaaca agtgccagat aacgctatgg
    70621 tatttgatga tgttgctatt acagtggaaa aagcagacgg ggaaacttgc gatcgctgtc
    70681 gccaagttcg taaagatgta ggagtagatg aaaaacttcc tcatctatgc ggtcgttgtg
    70741 caaaaatcgt tgaagacttc tatcctgaag ctgtagctga aggattcgaa gaaaagtaaa
    70801 agggaaaagg gggtgtgaca tgttctttgt cacaccccct tttgttatca atcgtttaat
    70861 tttccacgtt cacgatacca gttatctggt tgccagatcc attcgaatcc gtctttttca
    70921 agtagttcga aagctgcttt aggtcccatt gatccagctt tgtagttggg aaaatccggt
    70981 tgtgtttggt cccaagcttt tcgaatgaca tccacgatat gccaagattg agcgacttct
    71041 tcccaatgag agaagttcgt tccatcacca ttcaacgcat ctaatagtaa tttttcgtaa
    71101 gcttctgggc tattttcaac catttctgca ctatttctaa attccaattt gattggttcc
    71161 gtttcaaatc cttgccctac ttctttccca ttcaaggata aagaaaatcc ttcagttggc
    71221 tgaatataga tcgttagaac attaggtgga agtgtcgtat cgtcacatgg ttcatcgaca
    71281 gatgttttga atacattaac aggaacttgt ttgaatacga tgttgatccg agtccctttt
    71341 tcagtcaaac gtttgcctgt acgtacatag aaaggaacac ctgaccaacg gaagttatcg
    71401 atagtgaatt tgcctgcgac gaatgtttca gtctctgatt gagcatccac tttgtcttct
    71461 tcgcgatagc ccttgaattg tacaccatcc aattcacctt gagcatattg cccgcgaacg
    71521 aagttttcta acacttcttc ttcgttataa atgcggatag ctttcaatgc tttgatcttt
    71581 tctgtacgga tttctttttc ggaaaatgct actggcggtt ccattgcaag taatgctaca
    71641 acctgtaaaa tatgattttg caccatgtct ttaagtgcgc cactatgatc gtagtagccc
    71701 ccgcgatctt ctacaccgag gttttcgcca aaagtaattt ggatattgtc gatatagcga
    71761 ttgttccatt gagcctcaaa aatattattg gcaaaacgga tagctgagat gttttggatc
    71821 atttcttttc ctaaataatg atcaatcctg aaaatatctt gttctgggaa tacttctctg
    71881 atttgctggt tcaattcaaa agcagactgg taatcagaac cgaacggttt ttctatgatc
    71941 aaacgattga agccattttt tgtcataata ttttgtgatt tcaaatgaga aacgatcgtt
    72001 ccaaaaaatt gaggtgccat agccaaataa tagacacggt taccgtttaa ttgatagtgt
    72061 ccatctaatc ggtcagacaa ttcttttaat gtctgataat gcgttgaatc atttacatca
    72121 tgggattgat agtaaaaatg gcttgcaaag gtagttgctt cttcatctga aggattcaat
    72181 gcacggattg tttctttgac aacttcacgg tagtgctcat cgctccaggg acggcgagcg
    72241 gttccgatta cagcaaaatg ctcgcctaaa tttccttttc tgtaaaggcg gaacaaagac
    72301 gggtacaatt tacgctgggc taggtcacct gtaccgccga atatcgtaaa caatacattt
    72361 ttttcattag acatgtggat acactccttt atattttatc gacaaaaatc gtgctggccg
    72421 ccttaaagct gacggggatt ttctttctat cattttggat agtgatcggt ccttcataag
    72481 ctgcgatgtc aacgatctca tattcttcgt tgatattcag gtcaatagtg actaagtaat
    72541 ccaacaattc tttttcatca agcactcgtg cgatacgcac ttttgtaccg acaggatagg
    72601 actccaatgt ttgtcggcgt tcttcatgga caacttcctc ttttttagga atcgatcctc
    72661 catgaggaca atgttcagga tgattcaaat agttttctaa atgatctgca agatgttccg
    72721 aagtcacatg ttccaatact tcagcatcat catggacttc attccagcta tagtctaaat
    72781 gttcaactaa aaaaacttcc cataagcgat gtttgcgaat cagactgcta gctttttgca
    72841 gtccgcttgc agtcaattgt acaccttgat aaggggaatg ttctacaagc tcttctttca
    72901 caagtttaga aatcatttca ctgacagatg cagctgagac atctagacta gatacgattt
    72961 gtttattgtt gactttcgtt gtatctccac ctagttctaa aatgattttc aaataatctt
    73021 ctcgatttgg tgtcatattc gatcatcctt tcatggtaaa aaaccatcat tagtaagtgt
    73081 aacaaaaaaa acgttaattg actatttttc taaataaaaa acaaaggatt tttagacgtt
    73141 tttacaacaa aaaacgaaag atgatagaga agactcattc tttcgtttat aaaggatata
    73201 tatatacatt gggagggatc tatttttcag ataacaactt atatgaagtg tctgcagatt
    73261 cgtcataaag agtttgttcg ggataagaaa agataagata aaaaccatac tgatcacgta
    73321 cgactaaacg atattcatct atttctacca cagatgtgcc aagagactga ccgttgatca
    73381 aaaacgtgta atcagatgta atctgtaatt gttgtttttg ctggctatca ctaaagaacc
    73441 atgtaccttg atactttgag agtccttctg acttttccgc ttgttttttc tttttcaagt
    73501 aaataaaact tgagagaacg gtaatgagtg aaccaagaaa atagatcatt ttttttatca
    73561 ccaaatcact cctttatgag aaaataatcg catagacata ttctttcttc tttatttcat
    73621 tgtaccgaat aaaagctaaa atttctctaa gactttcgac tggttttttt actgtttttc
    73681 tacaaattat gataggatac atagtaggaa gaaaggtgac ataatttgaa gtatcagcaa
    73741 gtggaaatag ctttttttat tgaccgcccg aatcggttta tagcacattg tatgaaaaaa
    73801 gacggagaaa taattattgc gcatgtcaaa aatacaggta gaggaaaaga ggtcctgata
    73861 ccaggcgctg aagtggctat tgtatttgct cctggaccta aacggaaaac cgcctatgat
    73921 ctgattgctg taaaaaagaa gggtgactgg tttaatatcg atagtcaatt gcctaatcgg
    73981 ctggcaattg atggtatttt agacggcacg atccttttac caggattaac tggtccattt
    74041 acggtataca agcgtgaagt aacttatctt cactccaaat ttgatattta tttagagacg
    74101 gtggtcggac aaaaagcatt tgttgaagtc aaaggtatga cacttgaaaa taaagcgatt
    74161 ggtgcttttc ctgatgcgcc cacactcaga ggattgaaac atataggaga attaacgtat
    74221 gccgcacaag atggttatgc cgcttatgtc ttgtttattg ctcagttcga acatcttcat
    74281 ctggcaacga tccatgaaga aatgcaacca gcactagctg atatggttcg acatgcgcag
    74341 caaagcggag ttcaaatcct cgcctataat tgtcaagtat cggcagatga ggtaactgtc
    74401 aagcaggcca ttccatttga tgtagatatc ccttttgaag atcctataca atagaaagaa
    74461 ggtaaaataa tgatcaactt aggtattatc ggaacaaatt ggattactca tcaatttgtc
    74521 caagcagctt taagcacaaa aaaatatgaa ttgacagcgg tatattctag acatttggaa
    74581 aaagcacagg aatttggttc tcattatgaa ggggatatcg aattcgcaac agatttgaag
    74641 acttttttca atattactca tatggacacc atttatattg cttctccgaa tagtcttcac
    74701 tttagtcaag ctaagcaagc aattttggca ggaaaaaatg tgatagttga aaagccagct
    74761 ttttcgacgc caaaagaaat ggatgaaatc atccatttgg caaatgaaca caatgtgtat
    74821 ttttttgaag cagcaagaaa tatccatgaa tcaggtttta aaaagattgc cgattactta
    74881 ccattaaaag acgagataat cggtgcaaat ttcagctata tgaaatattc ttcgcgctat
    74941 gaccaagtat tagatggaga agaaccgaat atcttctcga ctcatttctc aggtggtgca
    75001 ttgatggatt taggtgttta ccttgtatat gcagctgttg cttggtttgg aatgccaaaa
    75061 gaagtccatt atttcccaag aaagatttct actggtgtag atggtcttgg atgggggatc
    75121 ttacgttatg agaattttga tgtatcgatc cagccaggta aaatcggtga ttcttattta
    75181 ccgtcagaga tttattttga ctcaggaacg ctagtgatga atggagtcaa tgcaattgaa
    75241 caagcacaat ttcatgatcg cgcgcataaa gaaatcattg atttggcggt aacagctgca
    75301 gaaaatccaa tggcagatga agcagaggat tttgccaatg tcatggctca tccgaaagat
    75361 ccagcttggg gattgttgta tgaagagtgg atcgagctag ctagaaatgt gaaccaagtg
    75421 atttatgatt taagaaaaaa tggcggcatc aaattcgatg cagataatga ggaagatttt
    75481 taatgaacga atttgtacaa gtatttcccg aaaattggca gaaaaaatat gaggataaag
    75541 ggtttatctc accttctctt attcaacgag cagtttttca gccactaagt aataaacaaa
    75601 gtatagtagc ggtttctcct acaggttcag gtaaaacact ggcttatctt tggcctctac
    75661 tactaaacgt ggaaccaggt gaagcaagtg cgttggtcat ttttgcttct tctcaagaac
    75721 tagctattca agtagcagat gttgcacgtg aatggggaaa agacaaagag ttgaaagtcc
    75781 aatctttggt gggcggagcc aacgtcaaac gtcagattga gggtttgaag gaaaaaccag
    75841 aaatcttgat tggtacacca ggaagaatat tggaattgat gaaagcaaaa aaaataaaag
    75901 cacatcaagt gaaaacaatg gtatttgatg aagcagacca gctatttgac gctgggaaca
    75961 gccaaatcat tgatcaaatt cttcatcaag ctccaacaga gtatcagcta gctttttttt
    76021 cagcgacagc agatcgttcc cttgaaagca ttgaaaaaat cactgggaaa acgtttgaga
    76081 caattgacgt gacagcagag gacgattctc gaaaaggact gagacattac tttttacgtg
    76141 taccgatacg aaagaaagat gactacttga gacgtctcac gcatatagaa gattttcatg
    76201 gtctgatttt ctttaatcaa ctgactgaat taggcgtaat ggaagaaaaa ttgctgtata
    76261 gaggtgtacc ggtaggaagt ctggcctctg atcagaacaa gctactccga aaagcagcgc
    76321 tagaacaatt caaaaagaaa atggtcagag cacttttgac cacagatgtg gcggctagag
    76381 gtttagatat tacaggtctt ccttatgtag tgaatgcaga agttccgtta agtgaagaag
    76441 cttatttgca tcgttcggga agaactggtc gaatgggtaa tgaaggaata gtaattacac
    76501 tcgtacaaga taataatcta agagatttaa aacggctttt acgtccatca aatataacac
    76561 ttgaagagat ttttttatac ggaggtaagc tgcaaactga gcagccaacc aaagaagatg
    76621 cagctcctgc agtaaataaa aagtatgcga aaactttttc tggaaaggag cagaacccag
    76681 aatataacaa agaaaaaata agaaaaaaag ttaaaaataa atcaaaaaaa gagagaaata
    76741 aaggagcaag gcgcaaataa gcgccttttt atgctatgat tatgaaaacg ggaacaaaat
    76801 tggttgccag aggaggggtt atgtgaaaga acgtcaagaa gagatcttgc agctattgac
    76861 agaaaaccaa caaggattga cagcaagtga tgtagctgag cgcttaacta tcgatcgaag
    76921 caatgcaagc cgatatttaa gtgaactata caaggcccat catattgtaa aaaccgctgg
    76981 ccgaccagtc gtttactctt tgccaacgga aaagtcaaaa tctgatgaag tgcatgtcga
    77041 ctcttctacg caagtgactt ttgaaacact cgttggagaa aatgattctt taaaagtcag
    77101 catacagcag gcaaaagctg cgattcttta tccaccgcgg gggttgcata cgattatttt
    77161 tggagaaact ggtacaggga agtcgatgtt tgctgaatgt atgtatcatt ttgcaatcga
    77221 ttcagagatg ctttctgcag atgcgccatt tgtttctttc aattgtgcgg attatgctca
    77281 aaatccgcaa ctgctctttg gacacatctt tgggatcaaa aaaggggctt acacaggagc
    77341 agctcaagat agtcccggtc taatagccaa agcagacggc ggtatattgt ttttagatga
    77401 gatccaccgt ttaccgccgg aagggcagga aatgctgttt actttcattg ataaaggtat
    77461 ttaccgtccg cttggagaaa gtgcacaagt acatgaagca tctgttcaga tcattggggc
    77521 gacgactgaa tcttcagaaa gtttcttatc tacgttcaat cgacggattc ctatggcaat
    77581 caccttgccg aatcttgctt cacgttcact ggatgagcgt tatgaaatca tctctctttt
    77641 tatcaaacaa gaagcgaatc gtttgaatca acgtatcgat atcgaaaaag aagcgattct
    77701 tgccttcatg ctttatgatg cggaaggaaa tatcggtcaa gtgaaacgag acttgaaact
    77761 agtctgtgca aaatcttttt tacattaccg tactcatcat gagaaaaagc taatcattcg
    77821 aaaagaagaa ctttcccttc aagtacaaaa aggattactg aaaatcaagg aaataccgga
    77881 acgtctcgac cgattcatgg acagcaaaag tcagtatttg acatttgaac caggatttgc
    77941 tgatgttgtc tggtcacaag atccagaacg aaacatgcag gtctataacg atattgagga
    78001 aaaagtcact tctctttccg aaaccggtgt ggagaacatt gatctagaga ccctcatttc
    78061 aaaagatgtg gatgcttatt tccagacata tgtcaaagaa ttgactaaaa gttcgattcc
    78121 caaagatctt ttgccagatg aaatctggcg gttaaccaat caattgtatg atgtggctga
    78181 aaaacggtta gaccgtattt ataatgaaaa agctcgtttt gcttttgctt tgcatctgca
    78241 aagtacattg gatcgtgtga gagaaggtca tatgatcgtc cacccagatt tgaataacgt
    78301 acggaaaaat tttaagaggg aattccaggt agcaatcgat ctttcaacga tcattgaaga
    78361 agaagaacaa gttgagattc cttttgatga aatcggcttt attagtatgt ttctttctat
    78421 caatctagga gaaagtgagc ctgttaaaga aaacaaagta gatgttctca tattgatgca
    78481 cggcagacag actgcttcaa gcatgttgga gatggcacaa gaactattag gaacaaaagt
    78541 agggaaagct tttaatatgc cactggttat ggaagttcaa acaatgtatg aagaagtgtt
    78601 acaatatgtc aaaaactatc aagagcattt gacgaatggc ctactcttgt taacagatat
    78661 gggatcatta aacaccttca gtgatttgat ttatgaagag actggcattc ggacgaaagc
    78721 tatcacgatg acaagtacca tgattgtgat agaagcgata cggatggcaa gtgttggaag
    78781 aagcttagaa gatatttatc aaaatatcca gctttccttt gaatcgatcg tacgtgatca
    78841 gttcaaagcg gaaaagtctg aaactttaca gcgtaaaaaa gccgtaatcg tgacatgctt
    78901 tactggtgaa ggagtagctg ccaaattata tcagcgtatt atgccagtga ttaatcaaaa
    78961 taaggtagaa ctgatccaga tgcagttttt agaacgtgaa tcattcaaaa aacacatcga
    79021 tgccttattg gaagaatatg agatcaaagc aattgcggga acagttgatg ttgattatca
    79081 aaatatccca tttttttcag cctatgacgt ttttgatgat gaaaaactaa atattttaaa
    79141 gcgtatagct gatgatgaga tcccgacagc taaaattgtc gattcacttg ctggatcatt
    79201 aagcgatgtc ttttctattg atgaactgat ccgttcattg caaaagacag ttcagcagat
    79261 ccagaccgat ttgtcgatta tcgtggaacc tggagtagat actggaatcg ttatccattt
    79321 agccttcatg ttagacggat tgatcaaaca agaaagccat cggcaatttg agaaactgtc
    79381 tattttctca aaaatttatc gtttggaaat ggatatcgtt cgggcaaact tgatggcact
    79441 agaacgaaaa tatcaggtaa aaattccaga agatgatatc gcatttctta cacaaatgtt
    79501 tcttgaaaat aaaataaaaa aatgaacaca ctaatcatac tgtttacaca ctaaacatct
    79561 gtgtgcgcac tataaaaaag agctaaaaag catgaaaacg ctatttcagc tctttttttt
    79621 attggcatgc ttcttgcgtt atataaaagt gtaaaaaaat aaaaggagtg ttaaaaatgg
    79681 caaaaaaaac aatcatgtta gtttgttcag cagggatgag tacgagttta ttggtcacaa
    79741 aaatgcaaaa agcagcagaa gaaaaaggga tggaagccga tatttttgcg gtctctgcct
    79801 cagatgcaga taataattta gaatctaaga atgtcgatgt attattatta ggtcctcaag
    79861 tacgtttcat gaaagctcaa tttgaacaaa aacttgcgcc taaaggtatt ccattagatg
    79921 tcatcaatat gcaagactat ggcatgatga acggagaaaa agttcttgca caagcagaaa
    79981 atctaatgaa ataaatcaag tatgcagttg ggggtataac aacgtggaag atcaacaaaa
    80041 tctagaagcc atcatggggt taatcatgtt tggcggtaat gcaaagagcg atgcaatgga
    80101 agcgattgcg gctgctaaaa tcggtgattt tgagttagcg gataggaaaa tcgctgatgc
    80161 agaagaatca cttatacaag cgcatcatgc acaaacagac atgttgacac aagaagcaca
    80221 aggaaatcat atgcaagtga cattattgac tgttcacagt caagatcact taatgacatc
    80281 tattgcgttt actgatttag caaaagaaat catcgaactt tatcgtcgcg tatacagcga
    80341 ataaatacaa cttctttttt gaacggaaga agaaaaattt ttgaacggat tttttatttt
    80401 gttataagga gaactctctt tatataaaaa acttatagga ggaagtaaaa aatgaacgga
    80461 ctatcaagtt ggatggagaa atacatcctg ccggttgccg caaaaatcgg agcgcaaaaa
    80521 catttagttg ctttgcgtga tgcttttatc ggcatactgc ccgcaacaat ggcgggatca
    80581 gtcgctgtaa tgatcaatgc aattattcgt gatttaccgc aacagtttaa cagtacctat
    80641 gctgacagtt tagcagcaga caaaggaatc tttgccgtat tgaatgtcat catcggaatc
    80701 aatggttttg tctggaacgg tacactagca atcgccggat tgatctttgc cttttcttgg
    80761 gggtataatc ttgcccgagc ttatggagta aatgatctag ctggggggat cgttggttta
    80821 gctaccttga ttcaaggtat ttcctttggt ccagcattat cagcagcatt agatgtgaat
    80881 gttccttcaa atgtcgcaga tgcaatcaat agttctgaga ttggtgcaac gattgcagat
    80941 ggcacactgt cagttggatt atggggttgg ctaaaactag atcatttgaa tggaaacgct
    81001 tattttacag ttatgatcat gggagctatt tcagtcatta ttttttgtaa attgatgctt
    81061 gccaacatta cgatcaaaat gcctgattca gtaccaccag cagtatctaa agcatttgca
    81121 gccattttac cagcgacgat cgctttatac gtcattgcta ttatcaatta tatcgtaggt
    81181 cgtgtagctg atgggcaact gatcattgat ttggtgcaaa aatatattgc agaaccattc
    81241 ctaggattat ctcaagggtt gggagcagta ttgatcgtca cagtatttgt tcaagtattc
    81301 tggttctttg gtattcatgg atcaaatgta ttggcaccag tgctagaggg aatctgggga
    81361 caagcacaac taatcaatat tgatattttc caaaaaggtt ataacggaaa aactggtaca
    81421 ccagccgtgt tagatgcaat cgaagatgga aaagcttata tgtgggtccg cggatcgttc
    81481 gatgcttttg catggtttgg cggatcaggc ggaacgatcg tcttactgat agctatcttg
    81541 attttctcta aacgtgcaga ttatctaaca gtagctaaat tatccattgg gcctggtatc
    81601 ttcaatatca acgaaccagt gatgtttggt ctgccaatcg tattgaatgc aatcatgttt
    81661 atcccattct tgattgctcc agtagtagca acagcaattg gatggacagc tacttatctt
    81721 ggcctagtcg ctccggtatc gcagcaagtc acatgggtag taccgccgtt cttattgtca
    81781 ttcttagcaa caggagcgga ttggcgggca ccgatcgtaa cattagtatg tatggtcgtg
    81841 actttcttga tttgggcacc atttgtcatc tctgcaaaca aaatggaccc tgccgcaggc
    81901 gaacaacaat aagatgaacg tgaggagcgg gacgctttaa gtttcgttcc tcataatgaa
    81961 ttttcgagga ggaactgaaa tgattacaat tgccagtgca atgcaacaag atgaaatcaa
    82021 agaattgtta gaaggataca atgaagaagg aatgactttt acatttaaag aaaaacaagg
    82081 aatcaagctg ttctttgaaa caagtgcaga agatccggaa gttgcggcta aaaaagcaaa
    82141 ggaactaatc aaagcacagc cttggggaac agtcctttat tttcaatcaa cagctgttta
    82201 gaaaatggtt taaaaagata ggatggttga gatgataaaa aacggtttat ttttatgtag
    82261 tattggttct ttgatcgtgt tgtatagttt gaatccacat tcgcaaaggt atgatttgta
    82321 tagtatggtc acaggtcttt ttctaacagg attaggaatc tatttcttca tgaaggggaa
    82381 aaaaagagaa gaagcaaagg agaaaaataa ctaatggaaa tcacgaaaaa attaaatatt
    82441 ccagcatcgt ttttttacga taaagttatg gactctgtcc ttttcgatgt acgtaaagcg
    82501 accggcaaat cagtaactag aaaacagctg aaaaatttgg agtacgtcaa acaattctcg
    82561 caaaataacc gggctcgcat aaaaatcgaa gaagtcattg aaaaccagtc ttataagttt
    82621 cgtacatcga caacgaagaa tgaattcgtc gttcattatc aaatcaaacc attggacgat
    82681 tcgacctgtg aggttcatta cacagaaaaa atggagtctt atggcatgct tcaaaagttg
    82741 aatgatatgc ttttaggaac aatcttgatg tattttaaac ggagacgttt caaaaaaatg
    82801 ctagatatga tcgaagcaac gtattaaatc aatgaaacat ggtactattt agttattgaa
    82861 aaatgaagta agctagaagg aatttttcat aggaagatag atctttcctg cgaatcctca
    82921 ctagcttttt tgtaggaagg ggcagcaaaa tgacaaacaa agtaagagag ctaggtgtat
    82981 cttcgattga ggaaatgttt gatttagcca cttatgcatt taatggggca gatacacctg
    83041 aaaggcgaga acgttttcgt acacttgcgg agaattcttg gaattacgga ttttttgatg
    83101 aggaggatcg gctaacaagt caagtaatgg cgaccccttt tccagtgaat ttttacggac
    83161 aagagtacct aatggccggc attggctatg ttgcttctta tccagaagca aggggacaag
    83221 gcgggatcaa tcagatcatg gaaaagatcc ttgaagattg ccgtgatcgt aaagtaagct
    83281 tatcttatct ggcgccattc tcctatccct tctatcggcg ctatggctat gaacaatcat
    83341 ttgataaaat cagttatcaa ctaaacagtc gtgatgtgcc gtttatcaaa aaaaaatctg
    83401 gaaaaataaa gcgtatggat tttgaagatg caaaagctgc aatcagaaga atttatgaac
    83461 agatgccgga aaaccaacga gctggtctga agcgcgaaga ctggtggtat acgtataaat
    83521 tcaagcaaaa aggcaattac cagtttgctt tgcattttga tgataatgag gaagcaacgg
    83581 gatatatcgt ttatcagctt gcaacgtctt cctttattat tgcggagtgg ggatttttgg
    83641 atcatgatgc tttttgcagc cttgttcgct tcgtaagttc tcacaatggt gcttttgaaa
    83701 catttgaata ttcatgcggc tacggcggta atgatcaaaa ttatctatta gaaaatccat
    83761 ttgcttctgt aatgatcaca ccttatatga tggcgaggat cgtagatatt tctttgtttt
    83821 tggaaaatta tccatttaaa aagagacaag ctgcatttgc cctacaaata gaagaagatc
    83881 attatgcgaa atggaatgaa ggaatcgtgg aagtaacaat cgaaaatgga aaaaatcatc
    83941 tctcaaaagt agaacaaaca aaactcccaa tcgttcgagg aagtatccaa catatcacgc
    84001 agctattatt agggtatcgt aaagtggaag agttggtatt ccaagaaaga atacaggtga
    84061 caaaagaata tgaagaaacg ttaagctcaa tcttgccaca gcaacagcca attcttaatg
    84121 actatttttg ataaaaaacg ggtgatgaca atatttttgt caccaccctt ttgtttttca
    84181 tttggcattt gaaggaaaat gttaaagtaa atcctctttt ttcgtaaatt tgaatttttt
    84241 gttttctttt gttttagggt tttctttttt gtggtcttgg attgcttctt tcgtaatctt
    84301 ttcccgattt tttttagatg aatctaagcg ttctgcgtat actttttctt ttggcttatc
    84361 attcattgga aaggacctcc ctacattatt tcattctacc tctagtttat catctgttaa
    84421 ggactagtgg aaacgttttg cctaacttat aagctgattt ttttagcaga taagcttgca
    84481 agtaaaaaac aaacaagcta caattgattt attgaaattg ttcctgtagc tcagtaggat
    84541 agagcaatcg cctcctaagc gataggtcgc tggttcaatt ccagtcagga acgtataaat
    84601 aacccctgta tcctttgcgg tacaggggtt tgttttgtat ttgtgccaaa aatgtgccaa
    84661 actagttatc atttttatat aaactatcga acacagtatt gattttttct ttatcctgat
    84721 cttcaagctc tcgaacaata tgaagataag tagacattgt agtttctaaa ctgctatgtc
    84781 ctaaacgttt cgatacgcta agtatattta ctccttgata aagtaaaata gatgcatgtg
    84841 tatgccttag tccatgcaaa gtaaattgct tttcaatccc taattctttt aattttttgc
    84901 gcaaatattt gctgacggcg tttgatgaaa ctaatccatt ttttaagttg aaaaaaacaa
    84961 aattatgagg gtttttgatt tcaaaattct cgaataattc tttttgattg attttaaact
    85021 tttttaagag atcaatcgta tgtttatcaa tggaaatttt cctatttgaa gtttcgtttt
    85081 ttgtttttcc ccattcattt aatttataat tccatgtttt attaatagtg atgatttgat
    85141 cttcgaaatc gatatcttcc catgttaatc ctagaagttc agcaaatcgc attccagtgg
    85201 caccagcaac taacacgagc ataggagaag agtactgcgc agaaaggttt tcttctacga
    85261 gtttcattaa agatttaaat tgatcataat ctaaatattt atcttctttt ggcttcaatg
    85321 aatcttttcc tttgattact gcttttctag ttggatcaaa cggaattaat ccttcttcta
    85381 cggcatcttt caacgacgct ctaatatggt tattaaactt aataacggta gattttacat
    85441 ggtctttagc atacttattt aaaaagcgtt gatatcccac tctatctaaa tcagaaatca
    85501 ataccgctgg catatatttc tttatgttca ttaacgtatc ttcgtatttt ctataagtga
    85561 taggagatac tgttccttct ttataaagtt gcatccaatc ctcaaagtaa tctgataata
    85621 atagattttt gcgatcccct ttaaggcctt tgttcaagtt atattctaat tcgttggcgg
    85681 catcttttgc ttctcctttt gtcttaaatc ctgattttct tatcttcgtg tattttccgt
    85741 catctttttt gtatgaaatt tcgtattgcc aactatttcc acgtttgact aatcttgcca
    85801 taattgaatc agctctcttt ctctgataca ataggaacta taaagaagcc cattgtttgg
    85861 gttttattat tttttgcaca tcctttttgc ttgccggcgc aaggatgtgc tatttgtcgt
    85921 tttaatggta taattggatg tgtattagat catccaatca gaggtgttta ttatgattaa
    85981 agtgattttt aaaagaataa ctgaacttcc agagtttaga ttatttgttg attattggta
    86041 tgtctggttt tttattgttg cattgctgat aggtctagat gtcatattag aaagaaaact
    86101 taagtaagag ttgagttaat aaattgaatt cctaaatgag atattttgta gcccatattt
    86161 tgtttgccga catcacctaa tactatggag ctaatcgttt tttctaacag accaaaatca
    86221 gataatctat ttaagctacc atctattagc tcttgtgaat agccagacat gctgatattt
    86281 ccagtgaaat tttcgtttcc acgactcatt aagcatgcta acaattgaat ctcaatcgat
    86341 gttaattgat tcagtacaag aagataatac tcctcgttat cccatgaagc gttgttaatt
    86401 cgaagtaaac aattcttata aagattttta tacatatcta ttttattttg tgcctttgat
    86461 ttttctacct cgtcatttat cccttcgaat attccaatga gttggtcctt gttctctaag
    86521 ttgaaaacat cttctggaat ttgatcgcga atgttttcta atgattcgtt taaatttttg
    86581 tagaatttct cgatgcgttt gaaacgcttc tcgtttttag acccaaaata aagtgactgc
    86641 acaacgccac caatataagg aattgctcca ataccgcctt caactattaa ttcaagatat
    86701 tcttttttgg ttagtttttc attgctatta tccatagtta cctcctattt tgttatttcc
    86761 tttacaagca ttgtgactcc tgatggcact cttttgtata ggttttatta ttgtgcacaa
    86821 tctaactctt agtggggcgt ggttcattta ttttttactt actttactat taatcatatc
    86881 ccattgagca taggattttt ctatgttcga tcctcttgaa ctaatttctc ctgtaaaagt
    86941 tactttatcg ccctttgtaa aggtatcagc attgtttaag tccttcgcaa aaattacata
    87001 tgaaacttta ggtgttgcag aaatatcatt ccaactttta tcatcgtact ctttgtctgc
    87061 tataatggct attctagtag tcattgattc gataacagtt ccttcaaaag tatatgtttt
    87121 accactaata tatttagtaa aagctgtatt ttgctcagta gctggagtaa tagaataata
    87181 actatctaag aaagcacccc agtcatgatt atttgtctct aaaaattctt caaattgagt
    87241 taggctttta ttgctgctgt tagatgtagt agtttgagta tcagatgatg agctttcctg
    87301 tttgttattg tcatttgcct cgtttgatga ggaaagcgag aaacctacgg tagtaaaaat
    87361 aatcccgata ataaggagaa tcataggtgt tttcttattt gtttttttga taaatcttac
    87421 aattaacatg attaaaccta taataaataa aacaatacca gtaaaaccaa atataccgtc
    87481 cattaataga tcctctttct cttttgatat tgaatattat aaaccaagca attctttttt
    87541 cttaaaatcg aattcttctt gattaataat cccttcatct aacaactctt tatacttttt
    87601 aatttcatct gcacttgaaa tttgtgtttc tttggaatta ttatctaata tagattgttg
    87661 cttctctata tattttttta aattttccat ttcattccaa tatttttttg taaacataac
    87721 ggtgttttca tctttcgtgg ccgctaatat acctccgcga ctttcgtttc cgcctagcaa
    87781 ggtaaattgt atataaccat tactcatacc tggttttttt aattgtacgg ctgatatatt
    87841 tttaaagggg atagtttttt ctcctttaat accttgatta actaaattta taaatccttt
    87901 tctaataatt gaaatagaat catcagttac tttaatcaag gtttttcctg gagattttat
    87961 taaaatctct ttttccattt tttcttcctc atttctgtga tatg
//
LOCUS       DACDYJ010000003        77896 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDYJ010000003 DACDYJ010000000
VERSION     DACDYJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.003"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(193..1560)
                     /gene="nhaC"
                     /locus_tag="IWM51_000195"
     CDS             complement(193..1560)
                     /gene="nhaC"
                     /locus_tag="IWM51_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9969643.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1670..3091)
                     /locus_tag="IWM51_000196"
     CDS             complement(1670..3091)
                     /locus_tag="IWM51_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9969644.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3334..5211)
                     /gene="tdc"
                     /locus_tag="IWM51_000197"
     CDS             complement(3334..5211)
                     /gene="tdc"
                     /locus_tag="IWM51_000197"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9969645.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5502..6758)
                     /locus_tag="IWM51_000198"
     CDS             complement(5502..6758)
                     /locus_tag="IWM51_000198"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP9969646.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7338..8639
                     /locus_tag="IWM51_000199"
     CDS             7338..8639
                     /locus_tag="IWM51_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9969647.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8708..9307)
                     /locus_tag="IWM51_000200"
     CDS             complement(8708..9307)
                     /locus_tag="IWM51_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9969648.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9561..10442
                     /locus_tag="IWM51_000201"
     CDS             9561..10442
                     /locus_tag="IWM51_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9969649.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10455..12065
                     /locus_tag="IWM51_000202"
     CDS             10455..12065
                     /locus_tag="IWM51_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP9969650.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12142..12801)
                     /locus_tag="IWM51_000203"
     CDS             complement(12142..12801)
                     /locus_tag="IWM51_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9969651.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(13084..13749)
                     /locus_tag="IWM51_000204"
     CDS             complement(13084..13749)
                     /locus_tag="IWM51_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969652.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13896..14501)
                     /gene="dhaL"
                     /locus_tag="IWM51_000205"
     CDS             complement(13896..14501)
                     /gene="dhaL"
                     /locus_tag="IWM51_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP9969653.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14529..15512)
                     /gene="dhaK"
                     /locus_tag="IWM51_000206"
     CDS             complement(14529..15512)
                     /gene="dhaK"
                     /locus_tag="IWM51_000206"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP9969654.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(15514..15909)
                     /gene="dhaM"
                     /locus_tag="IWM51_000207"
     CDS             complement(15514..15909)
                     /gene="dhaM"
                     /locus_tag="IWM51_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP9969655.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            16063..16383
                     /locus_tag="IWM51_000208"
     CDS             16063..16383
                     /locus_tag="IWM51_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP9969656.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(16495..17364)
                     /locus_tag="IWM51_000209"
     CDS             complement(16495..17364)
                     /locus_tag="IWM51_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9969657.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(17460..18734)
                     /locus_tag="IWM51_000210"
     CDS             complement(17460..18734)
                     /locus_tag="IWM51_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9969658.1"
                     /translation="MLRYLFVYYVFFLVSYPILGGFVWFIGVWCYVFLYKHKQKEWVD
                     VPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGSTDQTPEILARL
                     MKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDALIRYVNYFIRP
                     GARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLYAYSGANTMYR
                     KEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTLKMLYRQRKRW
                     AKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVLFAGLIGHYVY
                     QGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFAPLYMLLFWIV
                     NAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(18760..19269)
                     /locus_tag="IWM51_000211"
     CDS             complement(18760..19269)
                     /locus_tag="IWM51_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969659.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(19444..21579)
                     /locus_tag="IWM51_000212"
     CDS             complement(19444..21579)
                     /locus_tag="IWM51_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9969660.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(21662..23317)
                     /locus_tag="IWM51_000213"
     CDS             complement(21662..23317)
                     /locus_tag="IWM51_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9969661.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(23319..23633)
                     /locus_tag="IWM51_000214"
     CDS             complement(23319..23633)
                     /locus_tag="IWM51_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9969662.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            23803..24063
                     /locus_tag="IWM51_000215"
     CDS             23803..24063
                     /locus_tag="IWM51_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP9969663.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            24077..24391
                     /locus_tag="IWM51_000216"
     CDS             24077..24391
                     /locus_tag="IWM51_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP9969664.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(25121..25675)
                     /locus_tag="IWM51_000217"
     CDS             complement(25121..25675)
                     /locus_tag="IWM51_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP9969665.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(25801..27729)
                     /locus_tag="IWM51_000218"
     CDS             complement(25801..27729)
                     /locus_tag="IWM51_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9969666.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(28035..28751)
                     /locus_tag="IWM51_000219"
     CDS             complement(28035..28751)
                     /locus_tag="IWM51_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9969667.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(28752..30575)
                     /gene="glpO"
                     /locus_tag="IWM51_000220"
     CDS             complement(28752..30575)
                     /gene="glpO"
                     /locus_tag="IWM51_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP9969668.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(30589..32085)
                     /gene="glpK"
                     /locus_tag="IWM51_000221"
     CDS             complement(30589..32085)
                     /gene="glpK"
                     /locus_tag="IWM51_000221"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP9969669.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(32221..33681)
                     /locus_tag="IWM51_000222"
     CDS             complement(32221..33681)
                     /locus_tag="IWM51_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9969670.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(33671..34966)
                     /locus_tag="IWM51_000223"
     CDS             complement(33671..34966)
                     /locus_tag="IWM51_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9969671.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(35298..35975)
                     /locus_tag="IWM51_000224"
     CDS             complement(35298..35975)
                     /locus_tag="IWM51_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP9969672.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(36005..37096)
                     /locus_tag="IWM51_000225"
     CDS             complement(36005..37096)
                     /locus_tag="IWM51_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP9969673.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(37197..37631)
                     /locus_tag="IWM51_000226"
     CDS             complement(37197..37631)
                     /locus_tag="IWM51_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9969674.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(38215..39672)
                     /gene="ascB"
                     /locus_tag="IWM51_000227"
     CDS             complement(38215..39672)
                     /gene="ascB"
                     /locus_tag="IWM51_000227"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9969675.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(39669..41564)
                     /locus_tag="IWM51_000228"
     CDS             complement(39669..41564)
                     /locus_tag="IWM51_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9969676.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(41622..43397)
                     /locus_tag="IWM51_000229"
     CDS             complement(41622..43397)
                     /locus_tag="IWM51_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9969677.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(43447..44943)
                     /locus_tag="IWM51_000230"
     CDS             complement(43447..44943)
                     /locus_tag="IWM51_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9969678.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(45137..46759)
                     /locus_tag="IWM51_000231"
     CDS             complement(45137..46759)
                     /locus_tag="IWM51_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9969679.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(47208..48521)
                     /locus_tag="IWM51_000232"
     CDS             complement(47208..48521)
                     /locus_tag="IWM51_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9969680.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(48766..48990)
                     /locus_tag="IWM51_000233"
     CDS             complement(48766..48990)
                     /locus_tag="IWM51_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969681.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(49010..49378)
                     /locus_tag="IWM51_000234"
     CDS             complement(49010..49378)
                     /locus_tag="IWM51_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9969682.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(49575..50285)
                     /locus_tag="IWM51_000235"
     CDS             complement(49575..50285)
                     /locus_tag="IWM51_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969683.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(50303..50800)
                     /locus_tag="IWM51_000236"
     CDS             complement(50303..50800)
                     /locus_tag="IWM51_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9969684.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(50837..51289)
                     /locus_tag="IWM51_000237"
     CDS             complement(50837..51289)
                     /locus_tag="IWM51_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9969685.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(51424..52029)
                     /locus_tag="IWM51_000238"
     CDS             complement(51424..52029)
                     /locus_tag="IWM51_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9969686.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(52479..53099)
                     /locus_tag="IWM51_000239"
     CDS             complement(52479..53099)
                     /locus_tag="IWM51_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9969687.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(53101..53970)
                     /locus_tag="IWM51_000240"
     CDS             complement(53101..53970)
                     /locus_tag="IWM51_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP9969688.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(53993..54400)
                     /locus_tag="IWM51_000241"
     CDS             complement(53993..54400)
                     /locus_tag="IWM51_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9969689.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(54419..55708)
                     /locus_tag="IWM51_000242"
     CDS             complement(54419..55708)
                     /locus_tag="IWM51_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9969690.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(55735..56037)
                     /locus_tag="IWM51_000243"
     CDS             complement(55735..56037)
                     /locus_tag="IWM51_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9969691.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(56062..58155)
                     /locus_tag="IWM51_000244"
     CDS             complement(56062..58155)
                     /locus_tag="IWM51_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9969692.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(58644..59408)
                     /locus_tag="IWM51_000245"
     CDS             complement(58644..59408)
                     /locus_tag="IWM51_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP9969693.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(59519..60388)
                     /gene="mdcB"
                     /locus_tag="IWM51_000246"
     CDS             complement(59519..60388)
                     /gene="mdcB"
                     /locus_tag="IWM51_000246"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP9969694.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(60413..61489)
                     /locus_tag="IWM51_000247"
     CDS             complement(60413..61489)
                     /locus_tag="IWM51_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP9969695.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(61513..62106)
                     /locus_tag="IWM51_000248"
     CDS             complement(61513..62106)
                     /locus_tag="IWM51_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP9969696.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(62110..63786)
                     /gene="mdcD"
                     /locus_tag="IWM51_000249"
     CDS             complement(62110..63786)
                     /gene="mdcD"
                     /locus_tag="IWM51_000249"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP9969697.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPLDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(63776..64075)
                     /locus_tag="IWM51_000250"
     CDS             complement(63776..64075)
                     /locus_tag="IWM51_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP9969698.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(64177..65823)
                     /gene="mdcA"
                     /locus_tag="IWM51_000251"
     CDS             complement(64177..65823)
                     /gene="mdcA"
                     /locus_tag="IWM51_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP9969699.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(65830..66741)
                     /locus_tag="IWM51_000252"
     CDS             complement(65830..66741)
                     /locus_tag="IWM51_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9969700.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            66861..67718
                     /locus_tag="IWM51_000253"
     CDS             66861..67718
                     /locus_tag="IWM51_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9969701.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(67905..68267)
                     /locus_tag="IWM51_000254"
     CDS             complement(67905..68267)
                     /locus_tag="IWM51_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969702.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            68613..70052
                     /locus_tag="IWM51_000255"
     CDS             68613..70052
                     /locus_tag="IWM51_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9969703.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(70059..72599)
                     /locus_tag="IWM51_000256"
     CDS             complement(70059..72599)
                     /locus_tag="IWM51_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP9969704.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQSLQQT
                     ILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPVGQQL
                     IGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(72713..74044)
                     /locus_tag="IWM51_000257"
     CDS             complement(72713..74044)
                     /locus_tag="IWM51_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9969705.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(74010..75488)
                     /locus_tag="IWM51_000258"
     CDS             complement(74010..75488)
                     /locus_tag="IWM51_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9969706.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(75793..77628)
                     /gene="tdc"
                     /locus_tag="IWM51_000259"
     CDS             complement(75793..77628)
                     /gene="tdc"
                     /locus_tag="IWM51_000259"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9969707.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        22352 a        16540 c        13382 g        25622 t
ORIGIN      
        1 tgtaaaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta
       61 taaacggcgg aagcagaagc aactccttcg gaaataagct gaaattcaca aaaatttaaa
      121 aagcaatttt cttgagtttc ttcttatttc ttggagttaa acgcttctgt cccagcctct
      181 tttttccttc cttcaatttt ttacacgtcg acgattcaat aaaaaaccgc ctaatattgt
      241 aaagacaggt gcaagaatcg agaaaaaggc gaatggcaag taatgcaacg cgctaacttt
      301 catcgttccc ataatgaatg tgccgctgac tccccacggt acgagagaat tgacggtcgc
      361 acctgaatcc gctagtgttc gtgtcaaata ggtttttgat aagccaacgt catcaaaaga
      421 acgcttgaat gtttctcctg gaagaatgat ggacagatac tgttctccaa cgatcagatt
      481 gattccgata cagctcaagg cagtaaaccc gactaaacga gaaggtcggt ccatcttttc
      541 ttttaattct tgaacgatgg tatcaacgat cagatatttg atcagtaacc cacctaatgc
      601 caatgcaagt aaaatcaaac tagcagaacc taacatactc atgatcccgc ctcgagaaag
      661 cagttcatct agctttggat caggtgtatt tgcgacataa ccattcatca agatgtctgc
      721 tgcttttccg accgtcaaac ttttctcatg gataaaagaa agaacgagag cagctgtcga
      781 tcccgctagt aatgtaggga tagccggaat ttttttgatt gcccccccaa ataaaataac
      841 tagtggaatc aatgtccaag gagagatcca aaagctcgct ttcatgtttt ggatcatttc
      901 attgatacta gctagatcgg ctgaatgact gttatgtcct aatatcgtat aaaaaatcaa
      961 cgaaaggacg aatgctggga aatccgtata acgcatacca gcgatatgtt cgaatagatt
     1021 aactcctcca atacccgctg ccaagttggt cgtatctgat aaaggcgagc agttattccc
     1081 taaaaaggca ccggaaacga ctgcgcctgt agtcattgca tcattgaatc caagtaaatg
     1141 accgatcccc aaaaaagcaa ttccaatcgt cgaaatcgtt gtaaatgaac tgccgacagt
     1201 cactccgaca attgcacaaa caagaaatgc tgtcggtaaa aagaaacgca cagaaacaag
     1261 tttaaatcca taaaccataa tcgtagggat ggttccagat aaaatccatg aagcaactaa
     1321 gacaccaatc aaaagaaaaa tcaggattgg aatgatccct ggagtgattc ctttgatgat
     1381 cccgtcatgg atctcatccc atgtgaactt tttaaatctg ccataaaaca acaaacacat
     1441 gaaagctgca aggatcggga tatgtggcga gagctggaat ccgatgatca gtactcctag
     1501 gagtgccaac aacacgatca atacagcgat actttctttt aaagaaatat ttttattcat
     1561 cgtcgatctc ctatttcaaa tgaaaaggcc ctgccatgaa aaaactattc acttggcagg
     1621 acctcctact aaatcagcga caaaacgtcg gctgaaagac ttattttttt tatttttctt
     1681 ctaaactttt ttctacctca atcccccaag aatctttctt gaagtgattg atgatcaatg
     1741 gaataacgaa catgaccact gtgatagctg ctactaataa tgcatcactc aatgtcgctg
     1801 tacctaatgt agcaaatacg acaacgatga tagagaagat cagcatgcag ctgactaccc
     1861 aagctaatcc gtttccgttt ttaccaattc ggtaaggacg ttcgaattct ggatgtttct
     1921 tacgcatttt aagcaatgaa acagcaatca aaatgtacac gatacaataa ataacagttg
     1981 tagcatttgt aagtgtcaag aatacagcac ttacgtcatc cattacacca taaagcaaag
     2041 caaataatga aataacgatt gattgtgtta gaacgacatt tctagatacg ccgtatttgt
     2101 tttctttatg gaatccgaat tttggtggaa ggaatccttc acgagctact tggatgattg
     2161 tttttgatgg tccagtaacc caagctgata attgtaaaag cacaccaata aataccatga
     2221 agctgaagat atttccgatc acttctggta agcctaggat ttgacagtca atcaagatag
     2281 gctgcgtgat atttgctaat tccatttttc catctggtac gacatttgct acaagcatcg
     2341 cattgatgac attcaataaa actaatccga tcaaagcgat aaatacgcct tttgtataat
     2401 tttttgttgc gtctttcaaa cgaggcatgt aaacagaact catttcgatc ccaacaaaaa
     2461 taaatgtgat accagctaag tatttcaatg aatcaatatg ttccaagtca gggaaagctt
     2521 ttgaccatga aaagtcacct agatagccgt ttgagactag tccgactttg aaggctgcca
     2581 aaacacctaa aacgaacatg atgactactg ggatatatac ccctagccat acaccgatgt
     2641 tcccgccgac ttttgccata tcgaatttca agttcaagat cgtgatgatc cagtagataa
     2701 ctaaaataca acctaaaacg aaccagtgat tattgccaag ttctacatta ccaaatgtgt
     2761 ttcctaacaa tggtcctaaa gtagaagcga ccattaccat acctgggaac atttgcaccc
     2821 ataatagcca tgcaacgaca aatccccatt tgctgcccag tgctgttttt acccataact
     2881 gaggaccgcc ttcttgagga agcatcgtgg aaagctcacc ggaaaccatt gatatcggac
     2941 cggcaaaaaa caaaactgca aaaattgaat aaaaaatcaa tgtccaaccg actgcggcta
     3001 acgtaggaat actccgaacc gttgcacaca gtgccattgt cagaccgata aaggtcccaa
     3061 gacccatttt acttacttca gatttactca ccgttgacac tctccttgat tttttggata
     3121 aaataacggt gtctttgaag tagtttcagc aataaacgaa acaattcagc gaatatttga
     3181 ggggattctt tgagtctatt gcttgaaggg tttcttcaaa gacaacctca aaacaaaggg
     3241 atcgaagagc ttaggtctcc ccttgctctt cagatccaaa catgcgtcag aaacagttag
     3301 tattcaagct gttttctaac atgattacct tgcttatttt gcttcgcttg ccaataattg
     3361 gtctgcgtag attttttcca atttttcttg aagagctgct ttgatttttt cagcatattc
     3421 ttcaaagtgt tcttctttgt tcatgtatgg tgtcattacg ctggcacgca atacagttac
     3481 ttttccggca cggttccatt cttcatctga gaatcctagt tggttcacaa attgtaatgg
     3541 gctgttgcca tagtctggga tagcaaagtc agtatgtgaa gttaagaact cattaccgta
     3601 aatgcttcct ttcacgtatg aagaataatc atatacgtca tggttcaatt tattcattgc
     3661 aactaagtca tcgttgcctt tttctttgaa tacatagtct accatattga aatctggata
     3721 agttaatgga tgaacttcga tttctttgtc tcctacttta aatgacaagt cattcaagaa
     3781 gttgtagaaa cgatgtgcac cttcgattga tgcacccatt aatttaccat atcctgtgac
     3841 attcaaaggt aagacatggt gtgctgccca aacgctggct gctgttgccc ctgcttttga
     3901 accttccaag atataagccc caagtaatgc tgggatatct gcgccttttt caaatacata
     3961 tgtcgcaaag taagagataa cgtctctcat gcggatatct ttgatgacga taccaccggc
     4021 agaatatgga acatagccca ttttatgtgg gtcaatcgtt acagattctg cttcttcgat
     4081 tgctttatat gcactgtgta cttcttctaa gatataatct ttgttttctg taaagacatt
     4141 gtatttgtaa tgaacatctt ttaaatcttc aaatggaatg aagttgttgt cttcgtctaa
     4201 gaaaatcgca cggccgtatc cgccataagc agcatctacg tgtaagtaga agtaaatacc
     4261 gtctttttct aatacgcgac gcaatgcaac gattttatcg ataccgtcga ttgctccttc
     4321 ttctgttgat cctacaacac cgactacacc taggattggt gttttttcag ctgctagacc
     4381 acgaacgatt ttttcaagtt cgttgatgtc catgcgatag ttgtggtcaa ctggcactgg
     4441 aatcacttgg tctaaaccga taccgatgat gtcggcagct tttagccatg aatagtgttt
     4501 agtttgtgga accaaccatt ttcctaattt ttctaaatgt tttccgctac gtgctgagtg
     4561 agctttgatt tcgtcgattt tttctgggac gctgtctaac agattcatga tttcttctgt
     4621 tgacaagttc attaattccc aatcgctttt tccagcaact agttcaggtg tcacttcttt
     4681 cattgcaagc ggcaatgatt tgatgttgcg ggcataccaa agtccttcta agtttgctaa
     4741 agaaccatca gcaacgatgt ggccccagcc atctttatag ctcattaatt ttgcaaattc
     4801 cattcctact tcttcttcca tttggcttgt tgcaggagaa gattcatagg caacgttatt
     4861 accattccat agcatagcaa aattgtacgc tagtaaagat ggcatcaatg tttcagagtt
     4921 catatgaccc cagtaacgtc cggcattatg ccaaggaacg gaatgggtac gcatccgcgc
     4981 tgaaatttct gatagaacat cctttgtttt attcacagtg tgttcaaaac tagcactgct
     5041 tttttcttct ggcgtaataa taggcatgtc ttgaggcatg tagttttgac gccagcctaa
     5101 atgttcatcc accaattcat tcaacaaagc tttatagatt tggccgttct cagctttgtc
     5161 cccaatgaat agggcattta ggtttagatc tttcgacaat gattcactca tagtgaaacc
     5221 ctcctaaaaa aatatttaaa ttttgtcgag ccctacatta cacctgttct tctattctgt
     5281 aaactaagtt ttggcctgat ttttaatcat attttaatat gtatcaatta tccgaattgt
     5341 tcacactttt gaaataatta aaaaaaacga gggaatttac aaatgtaaac tccctcgcaa
     5401 agattgtatt aacgacaacc agcgttctat tttaggatgg gtttcaaaat tggaatgttg
     5461 taatagtatt tccctttttc aaggtaaaaa cttactaaat tctaatcaat tactttggcc
     5521 aaaaagtaat ttttcttacc ttttcgaata accacaaact taccatcgaa ttcatcggat
     5581 ggatttatga caaaatttaa atcggtaacg cggtcaccgt taatacttat agctccgttt
     5641 gaaacatctt cacgtgcttg acgtttagat ggttcaattt ttgtggaaac aagcagttcc
     5701 acgatatttt caggtgtgct tgaaatttca acgttcggca ttttaccgaa tccttgcgcg
     5761 atttcttccg catttagttc tttaatattt ccagagaaca atgcttgcgt tattttttgc
     5821 gcttctttca agtcttcttc actatgcaca aatcttgtaa cttcttctgc caaacggcgt
     5881 tgtgcttcac gtttctcagg ttctgtctct acttttttcg ctaagtcttc gatttcttct
     5941 tgagacaaga aagtaaagaa tttcaagtat ttcactacat cgcgatcatc ttgattcaac
     6001 cagaattggt agaactcaaa tggtgaagtt ttcttcggat caagccaaac ggcaccgcct
     6061 gcagtttttc cgaatttcgt tccgtcagcc ttcagcatca atgggatcgt taaaccgaac
     6121 gcttctactt ctggtccttc taattttcgg attaggtcta atccagctgt gatatttccc
     6181 cattggtctg ctccaccgat ttgcaattgc acatcgtatg ttttatgcag atgcaaaaag
     6241 tcgatagatt gcagaatttg gtaagtaaac tctgtaaatg agattcctac ttccaaacgg
     6301 ctggcaacga tatcttttgc taacatcgtg ttgatgttga agtttttacc gtagtctctc
     6361 aaaaattcaa gtaaagagat ttttgacaac cagtcgtagt tgttcacaaa tgtgatattt
     6421 gcatctttac caaacaattt tctcatttga ttggaaagtg cgtccacgtt atgttgcact
     6481 tgttccatcg tttgcaacac acgttctgtt ttgcggccgc ttgggtcgcc gatcgttcct
     6541 gttccgccgc caatcagaat atatggatga tggcctgcca attggaatct tttcatcatc
     6601 ataaatggaa tcaaatgtcc gatatgcatc gaatcaccag ttggatcaac gccgcaataa
     6661 agtgaaatgc tcttttcttc aacgagctcg cgaagtcttt cttcgtttgt ttgctgatta
     6721 atggcgtcgc gccaagttaa ttcatcaata atattcattt tactgacctc cgtgtagttt
     6781 ttttatgaaa gcaggaacct gctagcatgt gtaattaatt atttcttcag gataggatag
     6841 gaaataaaaa aagtcccttg gcatataagc caagggacga aatctcgcgg taccacccaa
     6901 tttgtatgtc tagatatcca acatacctct cgaacattta acgctgtcgc gtggcaatca
     6961 ctgccaaaga ctcctgaaat gtaattcgct ttaaagcatg tactggtttg cagcgaccac
     7021 cagctttctg aaacattggc tttaacaact acttcgtttc gatcttcgtc ttgttattat
     7081 taatatgtgt ctatcataga gcgcaacttt ttctttgtca acagaaaaag gatatttttt
     7141 ttaaattcct cattttttct tttttgaatc cgctctaatt ccgaacattt tattttcaat
     7201 tattgttaaa agaaataact ttttctgtat tttttctgca atttatcgag aagtcacttt
     7261 actttgcctc ttattctttc ttcgttacaa taaggaatgg gacaagtaaa aaaatgaaaa
     7321 agcaagggac gaggtatatg gaaaaaaagc aaaaaattat tttaggagcc acttctgctt
     7381 taactatcgt tattttaatt ctaagtttgt tttttgtcat ccgatcatct acaaaaaaag
     7441 aaccaatcat tcctgaaaaa aaaataacag aaaaagcaaa agaaccgaca gatgataagc
     7501 aacaactagt tactcaagca aaaaatctgg ctcaaagcta cgattatgac caagcgattg
     7561 cgttattgga aaaagatagt aagaacggat ctactgaaac acaacagctt cttcaaacac
     7621 taaaaaagga gaaagaacaa ctgatcgact ggtctgatcc tactcagatc tctcatgtct
     7681 ttttccacag cttgatcgtg gacccgcaaa aagccttcca ctctcaacag gcacaaggat
     7741 acaaagatta tatggtgact gttgaagaat tcaaccgttc gatcgaacaa ctttatcaaa
     7801 atgattttgt tttagttaat ttaaatgatt tgatccaaaa agacgaacaa ggaaatcttt
     7861 cctttaacgg gcttgcgcta cctgaaggaa aaaaaccatt ggttctttca caagatgatg
     7921 tcagctatta tgaatatatg gataattccg gttttcctag ccgattggtc gtatcaaata
     7981 acaatgaaat caaaaatgtc tataaagaga aagataaaga agaaatcggt gactttgaca
     8041 tggttcctct gatcgatgcc tttgtcaaga aacatcctga tttttcttat cgaggcgcaa
     8101 aaggtacctt ggccctgaca ggttacaacg gagttttagg ttatcgaaca tcaaaatctc
     8161 aatatggaga caatgaaaaa acaaaaaaag agattaaaga agctaaaaaa gtggcggaac
     8221 agttgaagaa agacggctgg agctttgctt cccatacatg gggtcacttg aatatgactc
     8281 aagcttcact agaagatgta aaaaaagaca atgaattgtg gcagcaagaa gttgcaccta
     8341 tcctaggaaa aacaaatatt ctgatttatc catttggtgc agacatcagc gactggcagc
     8401 cttattcagc agaaaatcaa aaatttgctt acttgaagag tcaaggtttc gatattttct
     8461 gtaatgtcga tgcttctaca ccagcttggg gacaattagg tacggatttc tatcgaaatg
     8521 ctcggatcaa tatcgatggc attcgttttc aatctgattt agacggaaaa aatcctattc
     8581 ttgatccatt catcaatgtc catgatgtct atgacaaaga agcacgcgaa tctgcttgat
     8641 tttacgtcat ttatgaacca aaaaagccga actgacattt tatcgtcagt ttcggctttt
     8701 ttatgcttta tttcttcttc ggtgcgattc ccttgatcag gacttctgtc cattctgctg
     8761 tatatggaag cagttttgcg tatatctccg gcttagaaat tccttctacc attgcttgaa
     8821 aatagaggat caagaactca tccgaatatt tcaaatccac ttttccttct ttccgccctc
     8881 gatggaaaag atcaagcatg atactgaaac tttcttgcgt atacgcatcc atcgcgtcag
     8941 aaaattcttt tttttctttt tttgtatagt atgtcatcaa atcgatataa aattgtttgt
     9001 tcactcgatt gagataacgg attttgtttt cactcatggc aatcagtgtt tcttcaaatg
     9061 gaagattttc cgcaatcaac gctttcgctt cttgccctaa atcattcata aatgcaaaaa
     9121 atacttcata aatcagccct tccttgctgc caaaatattt aaaaatcgtc gctttactta
     9181 cctgagcgtt ctcagctacc ttatccattg tcactccagc ggttcctatg tctgtgttca
     9241 tcaagtcaaa agctgcttgt aaaatctctt tctttttctc ttgtgctctc ttttcaaaac
     9301 cgttcatttg tagagcctcc cgtaatttta aacttttata atatatatag ttcaaattta
     9361 ttctgaggtt tattataact atctattgag aaagtatcaa gctagcaatt ccttgtttat
     9421 gaattcattg acagaaattt ataaaaagaa tattatgtac taaaataata aaaaaagttt
     9481 tataaaaagg tataaaacct ttttattaaa ctatttaatc ttgtttagta tatattttta
     9541 gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt cacacatttg aaaaagagct
     9601 ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt acattcaggt gaagtcgttg
     9661 gatttatcgg accaaatggt tctggaaaat ccacgacgat tcggacactt ttagggatta
     9721 taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga tgtatgggaa gatagtttat
     9781 ctatccatga acgtctttct tatgttccag gcgatatctc tttgtgggga aatttgactg
     9841 gaggagcgat catcgatctc ttcatgaagc ttcatggaaa agggagtgct caaaaaaaag
     9901 atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa agcaaaaaac tattcaaaag
     9961 gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt tgattcggat ctctatttat
    10021 ttgatgagcc tacttctggt ttagatcctt tgatggaaac gattttccaa gaagaagttg
    10081 aaaaattgaa agaacagggc aaatccatcc tactttcttc tcatattctc agtgaagtcg
    10141 agcgactagc ggataaagtc gtgatcatac gcgaaggaac agtcgtagaa gaaggaacac
    10201 tcgatgaatt gcgtcatttg actcgttcga ccatcactat agaaacagaa gcagagaata
    10261 gtcacttagc acaattgaac ggtgtccatg actttgtgca aaaaggtcac ctcgctcaat
    10321 ttgctgctga taacgaagct ttcgatgtga ttttagctga agcgtccaaa ttaggtgtga
    10381 aaaaatttga atctattcca ccgactcttg aggatttatt catgagacat tatgaaggat
    10441 gaggaggaag cgaaatgact gataaatttt cacgctggtc cacccttttt tctcaaaacc
    10501 ttagccgtga ctggaaaaag atcctgatat ggctagtcgg tgtcactgct ttttctggtg
    10561 gttttgtccc tgcgtttgaa gaattgactt ccggacaagg attagctgga ttatacgaaa
    10621 ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac tccagcttcg aatgctgctg
    10681 agtatacttt aggagcgatg tatgcaaatg aaatgttgct tttttgcagt ttgtttgctg
    10741 ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa aaaagaagaa cttggtctga
    10801 ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa ctctttagct gtcatcgctg
    10861 aaagtattct gatcaatttg cttttagcaa tatcgatcag ctgtacgatg atttttttcc
    10921 aagcagattc gatcactgca gaaggtgctt ggctttttgg gctatctgta ggtagtatgg
    10981 ggattttggg gagtgccgtt gcactgtttg ctgcacaact gatgccaagt tcagccagtt
    11041 cgaatggtct gtctttagcg tttttaggtg gactttacct tctacgagca ataacagatg
    11101 tagctgattc ctccctttcg atgtggaatc ctttaggctg gtcttatttg acctatccat
    11161 ttacaactaa caactggatt cctttgcttt atttgttttt gttcagtcta atcttgataa
    11221 tcagttcatt tgttttagag gaacatcgag atttgaacac cggctatatc cccgaacgtg
    11281 aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg tggtttatta ttctatctaa
    11341 ataaagggat gacaatcagt tggcttatca cttttggttt gatgggggcg gcatacggct
    11401 ctatctacgg gaatatgcaa cagtttttag aaagtaacga cttaatgaaa cagatgttta
    11461 tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac gatcatggtc gtcttgattt
    11521 gtctagctgc tattttacca attgctagca tcaatcgttt atttacagaa gaaacccacg
    11581 gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa ccaactttat tggaccaata
    11641 tgttactagc tattctattt gctatattgg caatcctgat tagttctggt gcactagggc
    11701 tttctgcttt ggcttcgatg aaggatgcga aagacatgga tctattgatg tttcttaaag
    11761 ccgggttcaa tttcctgcct gccgttttag tgtttatcgg aattagtggc tttctattcg
    11821 gatggcttcc tcgaatgggt aaacttgctt atatctactt agcttactcc tttgcactca
    11881 attactttgg cggaattttg gatttaccag aatggttttc caaaacagct gtccctagct
    11941 gggttccacg gatgccaaac gattcttttg atctttccgt gttccttacg ttatcatgta
    12001 tcagccttgc actgttcgtc cttggttcta ttggttatag gcgaagagac ttactagaag
    12061 gataataaaa aagcatgtac aatttcatgc tagcctccaa atctgggtca aaaatccaac
    12121 atttggaggt cattttttta tttacaagtc ttctaactgc tctttatcaa tgatccagac
    12181 ttctcgattc tttcttttta cataaccatt ctcctctaat aatttaaatt ttctagataa
    12241 agtttcgggg gtcgtaccga taaatgcagc aagttctttc attttcattg gcaggaaaaa
    12301 gtgatccttc cctgtcgctt tagctaagtc aagcagatag acagcgactc gttcttctac
    12361 tttttccatc atcaaaaaag ttgtctgctg ctgtactttt gttaatttct caacgttgat
    12421 tgctaaaagt ttcagactaa gggcgggata atgcagtaag agttccgaga aatctttttg
    12481 tctcaatatg caaatttccg ttttttctag tgcttcgcca aataaagact catttggaac
    12541 gcctagcaac tggctctctc cctcatatcc gcccggctct attactcgta aaagctgttc
    12601 ctttccgcta gcagataatt gatacacttt catactgccg gaggccacga tcactaattg
    12661 aggttctcca ttcggcgcaa gaatctgttc tcctttttcc acatagtgat gtttgacaag
    12721 tgcgtgtact ttttcttgat cttgctgtac taagtgattg aacaaaggaa caaatgaaac
    12781 acataaatga tcatgactca taatctctcc tccttctaaa cagctatttc taataaagaa
    12841 acataacttc ccttattcca actgataatt ttcttttatc atagcataaa cgtcaagcga
    12901 accagatacc agcaaataga gtggatttct ttttgtccct tgctttattt tccttctttt
    12961 gagaccttaa gtaattgatt cctatcaatt ttttaaatat ttactttttg aataatcaaa
    13021 aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt tcgcattgct tatttcgtgt
    13081 tgtctattga aatttttccg cacctagctt ttgatatagt ggtgctagtt cttgtaaggt
    13141 ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga tagaagaata gtttacccat
    13201 ttgaaaggtt cggtctctca tcgctttttt caactcttct tgtttctttg tatcggacaa
    13261 atagtcagac aaagctaagt ggtcgtcaag ttcatgttct ggttgcggcc atatgtaata
    13321 atccttgata tcgactgttt tgctccactc tttcagtatc gcaagcaact ggttatacgc
    13381 tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg tacatcaaat agatttggta
    13441 ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg tcttgacgag attcgcttcg
    13501 gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc gtccagcttt cgattttagg
    13561 tttgctcatg ccgtacatat caggcaaaag tgcagctgtc tggagctgaa tctctttcca
    13621 tttttgccag tgttctttat attctttctt gatttgttca atcttatctg ttggatggct
    13681 ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac aactgataat ctgattttcc
    13741 aagttccatt gtttcgcctc tttccgttat cttttgtatt ctacctgaat tttgcgatat
    13801 ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt cataagaaca gccaaacaaa
    13861 aaactgcgat gagcatcacc gcagttttat tgaacttaag gttgattgat cacatctaaa
    13921 agcgttgaaa ataaaatggc actagaagca gctcctgggt cgatatgtcc gattgcacgc
    13981 tctcctagat aggaagctcg tcctttttta gctaccaaga ctttggtgtg ttcttttgcc
    14041 tggtcaatga tttcttttgt taaattccct gccttcaatg cctctgtgac tggcagccaa
    14101 acatctaaca tcgttttatc gtcagcttct gcctttcctc ggctttgaat acccgctgtt
    14161 ccctgctcga tcacttcgcc taatttttct tgagaatcaa tagaatcgac ctcttttgtc
    14221 gctttcatca tgttcatgaa tgcagatccg tataacggac cagaagcccc accgactttc
    14281 gatatcatcg ccatagagaa tactttgaat gtatcgctga ttgtttcagg atgatcggct
    14341 tgaaaagctt cttcataagc tgaaacccct cttcccatgt tatttccatg gtctccatcg
    14401 ccaatcgggg tatctagatc actgagatat tgtttgttct gattgattgc ctctgcaaat
    14461 tgcgtgagcc attcttggat ttcttgaacg gttagtttca tattatcacc cttttatctt
    14521 gagatttttt accatgaaat cgtctgtaca ttgctttcta aagaatcttt ccattgtgtc
    14581 tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat ctagtgaggt cataaaatta
    14641 ccgacttttc ggaaaactac attgacgcct ctttcttcca acagagctaa aacatcattc
    14701 atgaacacga actgttccat caaaggcgtc ccgcccattc cgttgactag tacacctgct
    14761 tcttttggta aattcgagta atcatctaaa atcttagtaa caagttcctt tgccagtact
    14821 ttagaaggct gcatcttttc tcgacgatag cctggctcac cgtggatacc aacaccatac
    14881 tcgatctcat cttctcctag ttcgaatcct ggtttcccta cttctggtac agttgctgct
    14941 cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct tttctcccag ttctttcaac
    15001 tcagctaggg aagcaccgtt tcttgctgca tcgcccacga ttttgtgtac aaggacagta
    15061 cctgcaactc cgcgttttcc agcagtatac gtactatttt caacggcgat atcatcatct
    15121 acgatcacac tttctacttc aatatcgtcc atagcagcta attcctttgc catatcgaag
    15181 ttcaatgcat cccctgtata attcttaacg atcagcaaaa ctccttttcc actatccgct
    15241 tccttaatag ccgtctgaat ctgatctgga gttggagaag taaacacatc accaagcacc
    15301 gctgcactta acatcccttc ccctacaaac ccggcatggg atggttcatg tccgcttcct
    15361 ccaccagaaa ccaaaccaac ttgttccgct ttctgattct tggctacaac acgggaagat
    15421 tctacacgat gaacaagttc tggatagctt tttactaatc cttccagcat ttcttcgaca
    15481 atattgcttg gatcattcat gatttttttc aatttattcc catcctttct ttccggcatt
    15541 ttgtgcttct gttaagattt gctgagtatc atctgttata ccggcagtga tggctgctgc
    15601 aaaagctcct tccactaaag gcgcatccac cagatgataa cgttgctgct gctcttcttc
    15661 caacatgtct tttgccagtt ccgagttcaa taccgcgcta ccgatatccg caaaaacaaa
    15721 ataactagct gcatcgcttg attcattgac tgcttctacg attttcatcg ggtctgatcc
    15781 gatctcgcca tttattccgc cgagagaaaa aatttgcacc tcttcggatt tttgcatctg
    15841 ctcaatcatt tctttaatac catctgttac tttttgacta tgagaaataa gcaaaatact
    15901 ttttttcaca agaatcttcc tttcttttac atagttgcta acgattggtt tcgctttcat
    15961 taatgaacaa ttcaagtata taataaattt ctaattttgg gaagatatgt gtagtgcttc
    16021 ttgaagtttg ttatacttta attaaaagag aggagtgatc gaatgattac aacaacaacg
    16081 aacagtgtag agggaaaagc cgtagtagct tataaaggga tcgtttttgg tgaggtcatt
    16141 actgggatcc atgcatttaa agacttggag gcggggttgc gaaatatctt tggcggccgc
    16201 tcaaaaagct atgaaaacga actaatgggt gcaagagaag aagcacttgc cgaaatggcc
    16261 gaacgtgcaa aagcattagg agctgatgcg atcattggcg taaaaatgga ctatgaagta
    16321 ttaggcagcg acaatggcat gctgatggtc acatgtagtg gaacagctgt ctccacgaga
    16381 taagtagggg acatctgttt taataactaa agagagatgg gacatgtagt ggcactcaag
    16441 aattagattt ttcagtctaa cttcgaggta ctctaccttc ccatcctctc ttttttattt
    16501 ctttttgcgg atgattccgt aagctgctgc gactaacaca cccgcccctg ctaaggaata
    16561 actgacagaa gctgtttttt catttgtttt tggcaaagtc tgacttgttg atttggatgg
    16621 ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag gtcactgatt tttctcctgc
    16681 cgaatgcaaa atatctttta ttcgttggaa actagctgct aagtcagctt tttttgcttc
    16741 aaaatcatta gcaactgttt gtgcttcgct gatctttccg tggattgctt gactggccgc
    16801 tgtgatatcc gcttgagttt gacttagctt tccagaagct gtttgagcaa cctgatttaa
    16861 ttgctgattg aattcttttg cttgttggat cttcccgtca agatctgttt ggacagcttc
    16921 cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt gcacggtcat ttcccacttt
    16981 atttttaaga tcagctaaat cttgatcgat tgctgcttgg tcttctttca atttgttttt
    17041 tgcagcttct aatgcttctt cgagttcttt tcttttattt tccaagtctt gcaaatgatc
    17101 gatttggtca gaagaggtag aactatttat tgatgtatct tctgaagatg aaatcgtttc
    17161 tcctgaatta tctgtcggta cattggaagt catttctgtt tccgaggcac cgttttcagc
    17221 accagaagag cttatagttg ttgaaggttc tgtttgagca ggttcagaag tttccgcaaa
    17281 agctgtctgt cccaataaga aaatactgga aacagcaatt ggccagatta acggcttcat
    17341 tctttttttc atcacttttc ccatccttac tactttttaa ctaatttctt attataagca
    17401 aaaatgtttg gaagaaccat aaacacaaca ggctgtttgt ctaaaaaacg gtgcatcttt
    17461 tatttttttg tacgttccgg gcttttccat gtcccgcttc cataacctaa aatcgttttg
    17521 actgctggaa tgaaagtcgt cacgatcgtt atcgcgttca ctatccagaa caaaagcata
    17581 tatagtggtg cgaaaagaag gtatttcaat tttcttcgtc gatcatctac aatcagcgaa
    17641 gctagcaact ggaaaaatcc tgcgaccatt tcaaagcaga tgaacacaaa actgaatgta
    17701 aaagtaatat aaatctgttc gtagtttcct tgatatacat aatgcccgat taatccagca
    17761 aaaagaacta cggaaataca aaagaatatt gaccagacaa tactcaatgt ttgatcaatg
    17821 aagatgatcg ttcttccaat atgtttaaaa ggatggagca tcactttttt gaaattcgtc
    17881 agccagactt ctgttccacc ttttgcccaa cgttttcttt gtcgatatag catcttcaac
    17941 gtcacaggta cttccatgaa aaagataatc tcaggagcaa aaacagataa ccagtcattt
    18001 aactgatgat cccaagcaat actgatgtct tctgtcgctc gatcttgtct gaatccgccc
    18061 acatcgatca aggcttcttt tcgatacatc gtatttgctc cactgtacgc ataaagacca
    18121 ccgaaaacag ctgactgtgt tcgtttaatg atgcccacga tacttgagaa ttcaaccgtt
    18181 tgagattttg caatcaattt ggtacgattt tggacatcca tatttgcagt gaccgcagca
    18241 atatgtcttg caccagggcg aatgaaataa ttcacatacc taatcagtgc atctggctcc
    18301 ggtaccgtgt ctgcatcatt gcttaaaatc aatttccctt ttgcaaaggc aagtccgata
    18361 ttaaacgcgt gggcttttcc tttatttttt tcgatccgaa ccacacgaag attagcgtat
    18421 tttttcatca gtctagctaa tatttctggt gtttggtccg ttgaaccatc gtctgtcact
    18481 aatacctcat agttgtgata attcaatttc gtcatcaaat actcgattgt atcttcgatc
    18541 acaatttctt cattatgggc tggcaccatg atcgtgatga acggttcgac actcaaggga
    18601 acatctaccc attctttttg cttatgtttg tataaaaaca cataacacca gacaccaata
    18661 aaccatacaa atccgcctaa aatcggataa gaaacgagaa aaaatacata atagacaaac
    18721 aagtatctaa gaataaatta cctgtatggg tgattgagat catttcagtt ccaccccatt
    18781 ttctttatac aaatctgcaa tgaatgtcgt atctagattt ttctcttcgg agacagaata
    18841 aaaacggacc gtttctctct cttcttttgt tccaaaacgt tccgtataca cttcttcaag
    18901 taattcttgt ctttttttca atttttcatc ttcatattca aaagatttat ttactttttt
    18961 gtatagattt cgattgttcc acctagtcaa tacaaatgag gtaatcagga aactgaaaaa
    19021 aatcaataca aaaaagccga ttaagaaata cagcattttc ttttctgata aaaaagcgta
    19081 caaggaaaaa tccttggctc tttctggtct cgttaatggg aaaaagaccc atataaaagg
    19141 aagaacaacg atcatccatc ctaaaagagc cactaacgtt tgccaaatct ttaaccagta
    19201 attacctttt gaaaaatact tatctttcac caaaattgat gcaacttctt ctcttgttac
    19261 ttttttcata tcctcacctc acgttttttt gttacgacgt aggagtttac catacatttc
    19321 atttcttttc acataaagtg ttcctaaatt tttcctaaga aaaagaacaa aacatttctc
    19381 atcagcaaat gaaaaatatt ttgttctttt tatcagttgt tctttttatt ttttatctct
    19441 taattatgtt gctctttcca atacgacaat gcttttcatt cctctagata tcgccgtgta
    19501 catttgtctg cgtcttcttt gctcatctcc atcgttttca aatggatata agattacatg
    19561 atcaaattcc agtccttttg ctagactcaa aggaatgatt tggcagtttt ctaatccata
    19621 tgaatttttt ttaatcgatt cttgtagcgt ttgggtttcc tcttctgttt gggtgataac
    19681 tgccgctttt tctcctttta gagaaaccaa aatctcagct agtattttca acaattcttc
    19741 agaggatcga aaagagcggt attcgggttc ttttccttgc ggtcggatcg atacgatgtc
    19801 gatcttctct tgatccacag cataggtttt gaaaagctct gtgattgctc cgctagaacg
    19861 gtaactattt ctaagatcat accgatggat cggcagatgg tagtcgtcga aacaacgcat
    19921 aatatcagca aaagtagtgg aagaattaaa gattgcctga ttctcgtcac ctactaaagt
    19981 aaagtgagtt tttggaaata attcgatcaa tagacctaat tgtgccaggg tataatcttg
    20041 tacttcatca atcaatagat agcgaaatgc tggtacattt accttttcaa caagtaaatg
    20101 acgaatagct aatataatga ctgcttcatc aacagtacgt gtgggttgcc aagcataagc
    20161 tgagccatat cgtttttcat aaattctttc aaacaactga tgctcatcca cccatgccat
    20221 ttcttcgatt tgtctggtta tttttcgata ttttttttgt aaaagtttcc ttgcataagc
    20281 tacaatactt tgttccgaat catcagaaat caatttaccg aataattcca actgcctatc
    20341 ttctgtcaac gagagtacct gattctgcac agcaggactt tttgcttgtt tcaaaagatg
    20401 attcttccat tcgctcatca gtgccttttt cgttgcttga agcctatcga tcaatttagg
    20461 atgagcagga actttctcat atattttttc gatcgttttt ttagaaagga ctgtttttcc
    20521 atttttagta agtccacgga taaaattcgg atgatctaaa aacaaaggat ctgattgttt
    20581 tagattatcg ataaactttt tgctacgcaa acgttctgtc tgctccgata cattttctcc
    20641 cgtgatacgt ttgaaataag cctcttcacc ttctatttcc tctgctgaaa gctgtgaaac
    20701 cagctgtatg atggttagat tcagtggatt tctttcacct aatgatggta ggacattcga
    20761 aatatagtcg ataaaccgat gattaggtga tagaatcagc aagtcatcgg atgtcatcgt
    20821 atctcgatat aaatagagca gatacgcaat acgctgcatg atagcagagg ttttaccact
    20881 tcctgctacg ccatttacca agatattttg acttttcaca tcccgaatga ttgtgttttg
    20941 ttccgattgg atcgtagcag tgatagcctt catttcattg gtctcatctt ggcctaagac
    21001 ttctaataat acgtcatctt gaatagctac agacgtatca aaaaacttga ccaaacgatc
    21061 tctttccaaa agaaattgtc gtctttcttt aataaccgta tcgattttat tttgacgaac
    21121 cgtataagaa gatggaccaa tcatgttatt gtaaaaaagt tcggcaattg gtgaacgcca
    21181 atcataaaca agattttcat ctgcatcatc tgtgaaattt gctgtaccga tataaaagga
    21241 ttcttttggc tcattatcca gaaagtctac aacgactttt ccaaagtagg gacttttcaa
    21301 taaacgttcg atctttgcta gctgattttc tgcgtttttc tgtttgatat tcatctgatc
    21361 gatttctcta tttttcattt caatcatcga aaaagtatcc aggttatcta agtaactgtc
    21421 aaaattcaat cgaagctcgc cagccatttc tttcaacgac tggtttcctt ctatttttgc
    21481 cgtatccatc gattcccgca gttcgttttt tttatcaatt agcttttgat acacgttctt
    21541 cagatatgct tcttctaact gtttttgtgt gttgaccata cacattctcc tcttttttta
    21601 aagtggtgga accgattagt ttacctcttt taagagtcga atgcaagctt tttaacgagt
    21661 atcatttgtt aggatatatc agttcttcag gtcggacagt ttgatataaa gaaaatgcac
    21721 catcaaggtt ttttacgtgg tagccagact gcttcaagat tctttccgct agatagctac
    21781 gtaatccgct atgacagctg acgatatatt cttgttggct gtctaattca tttaatcggt
    21841 cccgaagttc attcaaaggg atatgtttgg cttccttgaa tttgcccttt tccaattctt
    21901 ctgctgttcg cacatctaat aaaatttttc cttttgctag ttctgtcggc aattcatgcc
    21961 attgtacggt ttcactaagc ccttctgcaa tgttcattgc tgcatatcct agcatgttta
    22021 ctggatcttt ggctgagcca aatggcggtg catacgtcaa ttctaattcc ggtaaatcga
    22081 agatcgtcaa atgccctttg attgctgttg ctaaaatatc gatccgttta tccacacctt
    22141 ttgccccaac accttgtgca ccataaatct cgccagtctc tggatggaag atcaacttca
    22201 gtaaaatatc tgttgcttct ggataatagc ttgcatgatc ttttcctgat acatgaatga
    22261 cttcaaaaga cagttcgttt tctttggctg ttctttcact taaaccggtg gatgcggcag
    22321 tcattccaaa cgttcgtata attgctgttc caataccgcc ttgatttctt cgagaaattc
    22381 cggaaatcgt gtccgcaact tgtctacctt gacgattggc tggtgaagca agagaaataa
    22441 gtgcatcttt tccagtgatt tgctgtttta caacgatagc atcccccacc gcataaatat
    22501 caggaatatt cgtttgataa cgttcgtcta ctaggatacc cccgcgtatg cctaattcga
    22561 ttccagcggt cactgctagc tggttatcag gacgaacacc aatggataaa ataactaaat
    22621 cagcagggat ggtacgccca tcatccagtc gcaatcgttt cccgtggtct tcaaatccag
    22681 cgacagcatg agaagtaatc acttctactc cttgatgaga caattctttt tctataaaag
    22741 ctgccatttc ttcatccagt gttggaagaa tatgtggtgc ttgttcgatc accatgactt
    22801 ctaaacctct tctttttagg ttttctgcca tttccaatcc gatgaatcct gcaccaacga
    22861 tcacggcacg ctttggctgt ttttctaatg catgcatcac tttatcgata tctggtacat
    22921 ttctaataga aaaaacattg tcggcttctg ttatccctgg aatcgatgga acaactggtt
    22981 ttgcaccagg agataaaacc aatgcatcgt actgttcaat gtgtttttga ccctcatgta
    23041 cgacttctac cgttttgttt tcgggaaaga tggctgtcac ttcatgattt ggacgcacat
    23101 ctaaacaaaa acgagcggat aatgattctg gcgtttggac aagaagattt tcccgttcac
    23161 tgatttctcc cgaaagataa taaggcaagc cgcaatttgc aaaagaaaca tacggtcctt
    23221 tttcaaaaac aacgatttct gcatcttcca ttaatcggcg aagccgtgta gcagcagaca
    23281 ttccacctgc tacacctccg acaataacga ttttcatctt atttccctcc tactgttcga
    23341 tcaaaccatt gattcatgcc accacgtaca ttcactacat cataaccaga tttctttaat
    23401 tctttagctg cttgtttact cctcatccct gattgacaaa tcacatagac ggtcttttct
    23461 ttgtctccat catatctgtt tatactttgt aatggaacat ttttagctcc ttttatatgc
    23521 ccgccccggt attctgcagg tgttctaaca tcaagtaacg ctggattttc caaaagcttt
    23581 tcctttaatt ctgggactgt tatcgatttt attgattgaa atagcaaatt cattttctgc
    23641 acctcattta catttttata aatacctcat ggggtatatt aaacaggagc tttattaata
    23701 tgtcaaccag aatgactctt catttttttc actacttttt atatttgcat aaaataccga
    23761 taaggggtat tatctaatta taaaaataga ggagaatgtg ttatggagat tgataataaa
    23821 aatatcctga accgtttgaa acgtacagag ggacaaattc gtggtatcca aaaaatgatc
    23881 gaagacgaaa aagaatgtat ggatgtcatc actcaattaa gcgctgttcg ttctagtatt
    23941 gatcgcgtaa tgggcatgat cgttgcagat aatctgaaaa attgctttga gaaacctgat
    24001 agtaatccag aagaacaagc ggctaaacta gaacaagcga tcaagatgat tattaaaaag
    24061 taaaggaatg atttcaatga aagtcgtttt ccatattgat gaattagaaa aatggtcaga
    24121 aacagggaaa aacgtaaaaa acctgataaa agcttctcca gaaacgacga ttgtcgtatc
    24181 tgtaaatggc atcgcgatta ccggttatct agactctgcc aatgcagaat tcttagattt
    24241 gcaaggagtc gtttttcacg cttgcgccaa tgctatgcgt gctaatcata tttcagaaag
    24301 ttctttacct gaacaagtaa tcgttgtacc agcaggtgtc cttgatttag ttgaactgca
    24361 atcccaaggt tatgcttata tcaagcctta gtcagtagag gttgcgacag aagtgatcag
    24421 cttcaagaaa tttcggctta tttttcgaag aagctacttc tggaaaaccg tgaatcaaag
    24481 gttgtgaaaa cgctgtcctg catcaagtaa taagaacagc caaacaaaaa tagcttctca
    24541 tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagga
    24601 agaggttgtg aaatcagcgg tttgacctaa gtattagaga aaaaaacggg aaaatagctc
    24661 ctcatatttt gccgtttttt gagctaatac cgaaggtcag ctgattgaac accgtttaag
    24721 gaagagattg tgaaaaagtc gcttagtttc gagtattaag aaaaattttg tgaaaatgga
    24781 tttctacatt tttgcataaa tttggcttaa taccgagaaa ctggcttttg aataccgttt
    24841 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagaaacaa acggaaaaat
    24901 agctgctcat attttgccgt tttttgagct aataccgaag gtcagctgat tgaacaccgt
    24961 ttaccaagag gttgtgagcc taccatttaa ctccgaatca caagaaataa tttcaaaaac
    25021 aacttctcct gtttttggtt tggttggctt attatcgaga agtagctact gggagtctat
    25081 ttatcaaaaa aaggaatgcg acataggtca cacccctttt ttattttctt tgaaacatct
    25141 ttcggtactc gacagcatac aagacaaaag attgtaaaag accgtaagtg atgacggcaa
    25201 aaatgactct cacccacagc gttggtaatt ctgggaaaag cagcaaaagg atcatgccaa
    25261 catagatgac agcagtcgct actttgccat accacttcgc tccacttaat ttgatatttt
    25321 tagatagccc gattaatcca gctacaaaca taaagccttc ttttatcaag aatagaccaa
    25381 acaacagcca aagccacggt cgataccatg ccatagacaa aatcagaaaa agttgtgtca
    25441 atttatctgc aaacggatca agcaccttcc ctaagtctgt tacctgatta tatctccgag
    25501 caatgacccc gtctagataa tcagacaaac ctgagacaac aatgaccgct cctgctatat
    25561 aaaatgactg tctcagcaca aaataaagat aaatcggcag caaaaaaata cggaatatcg
    25621 ataaaagatt cggaatcgtt tgtatttctt tacgccagtc tgtgcttaat ttcattttat
    25681 cgccctacct cctcagcact cttttctttt tattttagct tacacgggga ataaaaccaa
    25741 tgatttcact gtttttattt ttctttacag ccactgatat tatgagctaa tttgagataa
    25801 tcacttgctg ttttcatcgt ttactcttct ttcttcctgt cgtttgcgca tttcttttac
    25861 tagtacttta tgagcttttt ttgatcctcg ctctaggtca cgctgccgtt tacgtgtttt
    25921 ataatcgata aacagactgt ctaaatcgta acctgtcggg tacagatctt ctgctgaacg
    25981 caccagacga acacgctgac gaggcacgtg gattattttt ttgtctaaat atacatcaat
    26041 cgtatcttca cctatatctt cataaactaa cgcttctttt tggtattcat tgacaaatat
    26101 ccgatcacct tttgcaaaga gctggcgttc ttttttattc tccttcctat ttgtaactgc
    26161 tgtcttagga agaaattctt ttttctgtgt ccgataggct ttttccgcca agtaacgctc
    26221 tgcttgatgg atcaatttca tcgacatatc cattttatga gcaatccaca aagcttggct
    26281 ttctcctgtt tctccaaccc gcaactgata tttaggacga agtgcttccc gatcgaaagc
    26341 catagcagcc ggcacaaaat cttcatgatc tccggcgaat ttcttgattt ctccataatg
    26401 ggtcgtggca atgatcaatg ccccttgctc atacatagat tccatgattg cgatcgctag
    26461 tgcagcccct tcatttggct ctgttccact gccaatctca tctaaaagta ccaacgtatt
    26521 cctttttatc tttttcaaaa tagcagcgat gttttgcata tgaccagaaa atgtactcaa
    26581 cgcattttct aaattttggt ggtcaccgac atcgacgaaa atctcatcaa agacagcaag
    26641 ctctgtccct tcttttgccg gaacttgcat accgaacatc gccatcaaag tcaataaacc
    26701 gactgtcttc aacacgactg ttttgcctcc ggcatttgct ccggtgataa tcaagcctcg
    26761 atagtcttta ccgatttcaa catccaatgg taccgcatgc tcaggtaaaa atggatgtct
    26821 gccttgcttg ataatgatat gttccgattt attgattcta ggtgtgcttc cattgatttc
    26881 tcggctaaat tttgcccgag caaagatgat atctaacacc gtgatcgttt cgatcaataa
    26941 atcgatcact tcttcgtttt ctgcgatagc accagtcaat gccgccagga cttgatattc
    27001 ttcactgatt tcttcagctt ttaacaactg atagtgttca ttcaatttag aaatcacagt
    27061 tggttcaata aaaaccgtcg ttcttttatt tgattcatcg ataatcgttc catctatttt
    27121 atttttatag ctggctttga taggaatcgt atagtattcg cctttttgaa caatcattgc
    27181 ttcttggatc atttctttgt ttttcggatg acgcaagaat ttcagtaatt ttgactggat
    27241 ttctttttcg attagttgta attgtttgcg tactttacgc aaattacgtg acgcgtcgtc
    27301 actgacttta ttatttttta ttttctggtc aatcaactct tctacattta tcaaatcagg
    27361 aagatgtttg ctataagcaa acaacaaggg tgtctggtat tgattcttat caaaaaactt
    27421 tgtgatcatc cgactgcttc taagaaaatc cgcatattcg atcaaatcgg ttggctgcag
    27481 aatcaaccct tttttgactt gttctgtcag tgcatcgatc ctcggcaacc ccataaatgg
    27541 aacatgctga ctgctttcta agatcaagcg tgcttctctt gtttcttctt gacgatccaa
    27601 gacagtttgt aggtttgtcg agaccgtcat atcgctgatt cgttttttgc tatagtttcc
    27661 aattgctctt gcttggattt cctttttgat ccgatcaaat tgtaattgac taatcatttc
    27721 gttgtccata ttctctcctt ttcttgaaga gatacctgtc ccaaaataaa taagggccta
    27781 gagataaata aaaggataga acacagctta aaaaagtcat gtcctatcct tattagaata
    27841 caaagatatt gttttcctga caggtaccgt ttcataagca caccaaataa actttgttta
    27901 ttggctattc gacttactga ttagatacca gctacactca caaaacatac cccattcttc
    27961 taggtcttat ttattttcca cttaactata gcaagacaca ttttggttgt caaactttag
    28021 tatgttttat agatttacac cattaatgaa tagacaccta ccgcgataat tgctccgatc
    28081 attggacctg cgattggtac ccatgaatat gcccaatctg aagttccttt attagctatt
    28141 ggcaataccg catgtgctaa tcgaggacca aagtcccgag cagggttgat tgcatatcct
    28201 gttggccctc ctaatgaaag accgatggct aagatcaatc cacctacagc gaatacgttc
    28261 gttccaggtg caaattcgtt ttgtccaaaa gctagcaacc caagaactaa tacaaatgta
    28321 ccaattaatt ctgtgatcac atttgctgga taattccgta tagcaggacc tgttgcaaac
    28381 gttcctaaaa tcgcgctttc atctttcgtt gcattccaat gtggcaaata agaaagccaa
    28441 acaagaatgg cccctgcaaa agctcctaaa acttgcgcaa tgatgaaagg aacgacaaga
    28501 ttccaactaa ttgcccctgt catcgccata gctagagaga ctgcaggatt caaatgagct
    28561 gggcccatga atccagaaat atagacagcc attgttacag cgagtcccca accaaatgcg
    28621 atgacaatcc agccagaagc ctgtgcttta cttttgttta aattcacagc cgcacacaca
    28681 ccatctccaa gcaaaactag gatcagtgtc ccaataaact ctcccatgat ttgagtcata
    28741 tcactgttca tttatttctc cccctttagt tcccgcagat ctgattctgc aatcaatgct
    28801 tctaaacgtt tagtctgttg cgccttctcc tcttctgtcc attcaaaata tccagcaatt
    28861 gcatcgatca caggttgctt gatctcatca agctgatctc gttcaaacaa cagatgattt
    28921 gtacgacgaa tgagataatc gcctggggcc aaaaccattt cttcttctaa accataacgt
    28981 aaccgagctg actcagcgag actcaaacca ggataaggtg ccatttcttt tgctaattca
    29041 aagatacgtc gggcattagt cccgtaaaaa tcagcgatat aagtagcatc ttcttcctct
    29101 aaaccggctg ctactccttc ttttgctaat tctgtcactg tttcttctaa tttagttgga
    29161 tcgaagtttc cacctgaaat ctgatatttt ttagagtcga tttctttcgt ctctattccg
    29221 tattcttctt ttaataattg gcgtatcaat cgcaaagctc cttctgccat cttacggtaa
    29281 tcggtgattt tcccaccaga taaagtcagc aaaccatctg gttctctttc taaagaacta
    29341 cctctggaaa tcgtagaagg tgctttttca tcacggctgt tttccaaatg gttcaacacg
    29401 tcttctactt cagcacgaga aactttgttt tccttatatt cacttactgt atcgacaact
    29461 ttattgaagc tcttgtctga aatcgatcca ttatctccac cattgtaatc agaaccagaa
    29521 ttaccaatca acagtggacg aagtcctgcc cagcttgctt cgatatcatt caatgtgata
    29581 tttgcttctg gatagcgatg attgatcaca tccaatagat aatctacatc ttcttgtgtg
    29641 actttcggat ccgtaaaatc tccttggtaa tccgtatctg tcgtaccaaa gtaagtcttg
    29701 ttttctcttg gaatagcaaa aaccatccgc ccatcttgct ttcctgtatc gaagtatgtg
    29761 ggttgcggta caggcagttt tttcgcatct acaactaaat ggatcccttt agttggacgc
    29821 attttaggag agactggacg cgtaaaattc aagtttcgta ctttatctac ccaaggacca
    29881 ctcgtattga tcactaattt tgctttgatt tcgatcactt catctgtcag caggtcacga
    29941 gctttgacgc caacgatttg atcgccatca tataaaaagc caaccgcttt cattttactt
    30001 actagataag ccccatcttc ggcagccttt ttgatattat caatcactaa acgggcatca
    30061 ttgttgcgga aatctagata gacacccgcg ccttttagcc cttctttttt caaaaacggt
    30121 tctctttcca atacttcttc tggtgtcagc gtataattct catatttggt tcctgtcaca
    30181 tttgccagtt tgtcataaag gtccattgct actttgaccg agaacatatt gaaggttgtt
    30241 gctccttcgc cctcatagat tggcaaaagc attggatctg gttttgggat gtgtggggca
    30301 atactttgta cgactgcacg ttcaccaact gtgtcagcta ctacttccac atcaaatgtc
    30361 ttcagataac gaataccgcc atgaacaagt ttggtcgagc gagaggaagt cccttctgca
    30421 aaatcctgca tttcgatcaa tcctgttttg attcctgatg ctgctgactg gactgccaca
    30481 ccggcaccag tgattccgcc accgatgatc aacagatcca attcttcttg ctgcattttt
    30541 tgaatgctat cttgtcttgt cttgtttgaa aacatatgga tttcctcctt attcaaatgc
    30601 ttgggtagct tttactgctt ttttccaacc gttgtaaagt ttttctcggc gctcttcctc
    30661 catttgaact tcaaatagct tgccagcttc ataaaattcg cggatctcgt ctgtatcttt
    30721 ccaataaccg acagctaatc ctgctaaaaa tgctgcccct agcgccgttg tttccaagtt
    30781 ttctgctcgt tggatcggta cattcaagat atctgtttgg aattgcatca gatattcatt
    30841 gtttgctgca ccgccatcta ctttcaatac aggaatcttg attccagtgt catcttgcat
    30901 cgtatcgatg atatctctca cttgataagc aatcgactgt aaagtagctt taatgatatc
    30961 ttcttttgtc gtaccacgtg tcaagccaaa catagcccct cgtgctgcct gatcccaata
    31021 aggagcacct agcccaacga atgcaggaac aacataaacc tcatcttcgc ttgtagagcg
    31081 tttcgctgcg tcttctgaat cgctagcttt ttgcaacatt tgaagtccat ctctcagcca
    31141 ttgaatcgaa gaaccagcca caaaaatact tccttctaaa gcatagtaaa cctttccatt
    31201 tatgccgtaa ccaattgtcg tcaataaatt atttttggat aattgcggtt tttcaccggt
    31261 attcatgaca ataaatgaac ctgtaccata agtattcttg accatgcctg gttcgaatgc
    31321 catttgtccg aataatgcgg cttgctggtc tcctgccatc cctgaaattg gtgcttcgct
    31381 tccataaaaa tgataacctt gcgtataacc ataaacttct gagttagaag tgacttttgg
    31441 taacatcact cgaggaatat ttagtaaatc taaaatctcc tgatcccaat ctagctgatg
    31501 gatattgaac aacatcgtcc gactggcatt cgagtaatca gtaacatgtg cttgtccatc
    31561 ggtcagtttc catactaacc aagaatcgat cgttccaaac atcaattcgc ctttttcagc
    31621 tcgttcttgc gcgccttcta catgatcaag aatccagcgt atcttcgttg ctgagaaata
    31681 ggcatcgatc actaaccctg ttttttcgtg gaaaaattct tgatagcctt cattttttaa
    31741 gttatctgca attccagctg actgtctgga ttgccaaaca actgcatgat agattggacg
    31801 tcctgtatct ttttcccata ttactgttgt ttcacgctgg ttcgttatgc caatcccagc
    31861 aatctgattt ggtttgatcc ctgattcgat gaacgctccg gcaataactg actgtacaga
    31921 attccagatt tcatttgcat catgttccac ccaactttct tgcgggaaat attgcgtgaa
    31981 ctctttttga gaactgccga tgtgtcttcc tttcttgtca aagataattg ctcgtgagct
    32041 agttgtacct tggtcaatcg ccatgatata cgttgattct gtcattgtga ttccctccat
    32101 tatatgcaaa cgctttcttt aactactctt atcatactac tgttttaaaa aaagaaactg
    32161 tcaaaatttt attctttttt tctttttttg acagtttttt attatgaagc tattttttaa
    32221 tcaattcttt tcttttttca gcgtctctat tcttcttttc aatcgaataa tgtcgtaagg
    32281 tgacgcaaat tctgacagat aatattcaca ctgttttttg tttctctggc aaaatgtgat
    32341 caccaaatca tattctttac cttcttcata acttccaatc tcaataccgc cgattcccct
    32401 cagttctctg atcagatatt gttgaaaagc aatacgataa atcggcagac tttctaaatc
    32461 gattccaatc actactggtt ttgcaatata aaaatcgatc atcaaaagaa cattggcata
    32521 ctggatcatc aggtaactaa gctcactatc ttcagctctt ttcctcggca attgctgcac
    32581 cgctgtttcc tgtaatttcg ccagtaactg tgttaaggaa tgcccgagca ttttctgctg
    32641 ctgttccaaa agccgatcac gctcatagac ttcgatcttt ccaacaaaga aaaaccattc
    32701 attgttgatc tgggaaattt ggtagcctac tgccttttct tctttgattt tcaaacgacg
    32761 aggacgataa tgccataaca tcgtttcccg aatgtaagta tctaaaacag cggttggtaa
    32821 ctttttgctc cgtgtcaaat catactgata aaatatttct tcgtctacta tcgaaaaaga
    32881 aacaaaaaag ctgtaaaaca tcattgtttc gtatacattc agttctgctg ctgttcgact
    32941 aagatgcatc acgatgattg gatcgattgc ttgataaagc cggtccgact gatagagtaa
    33001 tttcttttct ttcaaagttg cgtacgtact tttttcattc aatagtcttt ttttcgtaat
    33061 cccgagccag caagcaatct gtgcttctgc tgatgccgta aacgttgttt ctaatacacg
    33121 gtccaatcct agaatgaacg gtcttttttc tggtgtattt tgcaaaaaaa gcggtcgctg
    33181 atcttccggg atatattgga atagttcata ataaaaatat cgtatctgta gctcttctcc
    33241 gtataattga ccgtttttga tttgtaatcc aaattcctct aagagctggt tcaattcttt
    33301 gatctttcga aaaacagaag actcactgat attaaacgct gtcgctaatc gaacgatgga
    33361 tacttgctga tgttctaata aatagacgag catcttaaac ttcaagcttt cctgcaataa
    33421 aaacaataga attttttcca aacttacatc ttcgtccaac acaagcagcg cctgatactc
    33481 atttcgctgt acttctactt ttttttccat cattcggcca atcaattcaa tatcttccaa
    33541 atattgatca aacgaggctt ttgacagttg gacatgctct ctcaaaccat tgatagaggc
    33601 tgttccccct tgcagaacaa ggtttcgcaa aatcaggatc tgtaactgct cttttctttc
    33661 taataatgct tcaatcttca taggcagtat tcgccacctt tgaccacacc atatctggca
    33721 cacggtattc tggctggaaa aatagctcat gttcttctaa ctgttcaaac gtccatcctt
    33781 cttctactgc aattgtcagt tggtcaagaa ggtggaacat ctcttttttg ctttggagct
    33841 gtgctccaag aatctttttt tcagaatcaa atagaaattt gagtttaact ttgctttgat
    33901 ttattgctgt aagcttgtag tctacgtatc ctgtttcaat tttttgggga taaaaaatgc
    33961 tctcttcttc tgtcaaacct acgcttccta agaaatagcc aaaccactga ttgccgactg
    34021 gacgataggt tccctgatcc tttgtctgtg agccactaat tgtcttgctg acatttcgtg
    34081 ctgtacggac agcgttattt actagagaaa catacatttt ttcatttgtt ggacgaaaag
    34141 ttacttggat cgcatcgcca atcgcataaa cgtctttttg agacgtttgc aagtagtcat
    34201 ctacccagat cgttccatca tcattcaatg ttaattgttc ttcccacatc tgattgtcgg
    34261 ggcgagaatg attagccagc aaaacaagat catagttctc ctttcttttt tccgtctcta
    34321 caatagcggt ttcattttcg gtcaaagaca caacttgttc ttctaaaaaa agtgaaagcc
    34381 cttgattttt catttccttt actaacggtt ctgtcatttc aggatctaga taacgaaaaa
    34441 gaattgactt tcggctttcg taaagatgga tttgtttttt tcttgatgcc aacccttcag
    34501 ctatttcgat tcctgcttgt cctgcaccaa taattgcaat tttctttgca ttcatgatct
    34561 tttgctgaag agccgttacg ttttgtatct ttttaactgg gtgaataagc gttgattctg
    34621 cagaaatatt tcgaaacttt tgatttgaac cagtcgctag aatcaaacga tcaaaatcca
    34681 atttttttcc tcctgctaaa acgacttttt tttctttcaa tcctattact gtcttttctg
    34741 tgtaaacatg gattcgatag cggtcagtca gttcttcttt cgtgatccaa gaaagttgtt
    34801 tttcactggt aacctttcct tttaatatca aattgaaccc gctgggaatc catcctaact
    34861 tcgattgctt ttcaatcaaa tatatttcac ttgttggcat caattggcga agataaatag
    34921 ccgcatgaat ccctgcaaac gacccgccga caattacaat gttcatctgc attcctcttt
    34981 cttataaggt gttctactgc taacttctca atcagaaaac acagtattca taactcgctt
    35041 tcaaccttat gatacatagc gatgcagcag aatcaaccct ctccatcata ccgtgttcta
    35101 ggtctgcttc tcaataatgt actagtggac ctaattgaag agtatcattt ctgaagagat
    35161 gtttcaaaaa atgtactctt ataaaagaaa aaaactcgcc ttttacgtac ctctgtccaa
    35221 ataggaaaca gaaaagaacg aaaagacgag ttttagcgtg tgtaatgtaa tagttagaat
    35281 agacacatca tttattttta attctttaat gcttcaacaa ctgcttcgcc taattgatga
    35341 gcagattgag gattttgccc tgtaagaagc cgcccatcaa tttgaaccac ttctgaaaaa
    35401 gctgctccag actgatagtt tgctcctttt tctttcaaag catcttctgc taagaatggt
    35461 acttcattcg ttgtaccatt tgcttcctct tcttcattac tgaacccagt cactgtacgt
    35521 ccagctaaaa tagattttcc agactcatct gtaactggca acaatcctac aacaccgtga
    35581 caaacagctg agaccagccc ttcatttcga tagatttgtg aagcgacttt cccaagatct
    35641 attgcttctg gaaaatccca cattgttccg tggccaccag cataataaac cgctgcataa
    35701 tcatttgcat ccacatcatc aggacgtaag gtatttccca aggcccgatt tctgaaatct
    35761 tcatcttcat aataggccca atcgatttca tccatattca aaacactttg cggttctaaa
    35821 ggtacatatc cgcctttagg actgacaaaa tctgcatcga tcccattttt tactaataca
    35881 tcataaaaat gagtcagttc acttaaccac aaacctgtag gacgctcgat cttttcgtac
    35941 ttttctacat ttgttaatac gatcaatact ttcattttcc catcctcttt ctataaaaat
    36001 tgcgttattg ataacgtgct ttcaagaaat tatataatgg gcttggcaaa taaagctctt
    36061 tatatgcttc ttcaaaaggt ttcgttacaa attcatcctc acggttttcc aataatttct
    36121 ctgcccagtc aggatcgacg atcatttgct gtccaattgc tgcaagttct gcatatttca
    36181 atacctgctc tacatcttcg cgattgcgga tcccaccgac accaactaaa gggatctttc
    36241 cttgaatcgt gttatggacc acttcaagaa tcgatttatc gttgtattta tcagaacgag
    36301 ctattcgatc atacatattt aatgaaacat gcagataatc tagttttgac tcacgtaatc
    36361 gttcaagaag ccagatcgtg tcttcaaaac ggatacctgg tgtttcatat tcttctggtg
    36421 agaaacgata ccctacgatg aatggttttt tagcatattg ttcaaccgct gagaaaaccg
    36481 ttgttaataa ttcttcaata aaacgataac gattttccaa gctaccgccc cattgatcct
    36541 ctcgtctatt cgaatgcgga gagaaaaatt gttggatcaa atacgtattt gctccatgca
    36601 actcgattcc atcaaatcct gattcaatcg ctctacgtgt ggctttgcca aatgcttgga
    36661 ttgtttcaac gatttcttct tcactcattg ctcgtggtgt ttccgcatca ggacgttttg
    36721 ctgcaactgc acttgcagat acaggctgtt ctccagataa agttgctcga cttgtcattc
    36781 gtccaccgtg gaaaatctga ataatcgctt ttgcgccttc tccttggatt gctttcgcta
    36841 gctcacttaa tcgaggaatc atttcatcat gagcgatgct gagttcacct ggccagcctt
    36901 ttccgatatc ttggacattt gcagcaccag taataacagc accaagacct ttagaacgtt
    36961 gtttataata agtgatctca tcttcggtga tcactccatt gaaaaagctg agttgtgtcg
    37021 tcataggcga cagcatcaat cggttcttca attgaactcc tgaaggtaaa gtaattgatt
    37081 gatcaaattg tgacattgtc attcctccca taattcgctc tgattaacct atcgagaatc
    37141 atctatcatt aaaccactcg attagttgta catgcaacta atttgcttaa aaaattttat
    37201 tttttttgta acttatctgt tgcatcattc attgtctggg ctaattcttt acttgcttca
    37261 tttcccaaaa ctgctttgta tgactgatga agttcttccc agctaccatc gacttcctta
    37321 gcaaattggc gtccttgttc tgtaagataa actaatgaca agcgaccttc gctaactgtt
    37381 ttgacgtatc ctttatattc taatttttca acaaaacgag caatcgttga tggggcggaa
    37441 tagagcattt gtgcaatatc tttttgtggt attccatctt tttcttttaa tatcaataaa
    37501 ataaaaccat aagttggtgc cagacctgtc cttctaaacg cttcatcagc taatttattc
    37561 aacatgcgat ctagcttttt cacgctaaag aaaagacatt catctaacgc tttttcaatt
    37621 ggcttttcca tttcatcccc tccattggcg tacatatcat agtatgattt ttattttttg
    37681 tcaaaaaaca cggaataatg tccgcctcct ctgtctagat ttgaaaaaaa gaagtagcca
    37741 tcttcaaaga atttcggctt gttttcgaca aaactccttc tggaacaccg tgaatcagag
    37801 gttgtgaaaa agtcgtttag ttttgagtat gaagaaaaat tttgtgaaaa tggctttcca
    37861 catttttgca taaatttggc ttaataccga aaaactggct tttgaacacc gtttatatca
    37921 gaggttgtga aaaagccgtc ctgcatcaag taataagaac agccaaacaa aaatagttcc
    37981 tcatattttg tcgttttttg agctaatgcc gatggtcagc tgattgtaca ccgtttattg
    38041 agaggtcgtg agcctgccgt tgaactccga gtcacaagga gcaatttcaa aaaacagctt
    38101 ctcatgtttt atgactatgg tgacttgtga tcgaggagtt ggcaggagaa tactgtttat
    38161 tcgtaaagaa ggggctgtga caagactttt gtcacagtcc cttatttcct tttattatag
    38221 gatttcgcca tttgattgga tgacttgtct gtaccaatca aatgattttt tctttaaccg
    38281 tttctttgtg ccattccctt cgtcatcttg atcaacatag atatatccat aacgttttga
    38341 catttgtgaa gtagaagcgc tgacgatatc aatgcagccc cacgacgtat aaccaaataa
    38401 atctactcca tctttgatag cttccttcat ctgctcaata tgctgtttca aatagtcaat
    38461 gcggtaatcg tcttgaacgg ttccatcttc ttctactttg tctatagctc cgagtccgtt
    38521 ttcagctatg aacagtggtt tttgatacct atcatataat tgattcaaag caattctcaa
    38581 acccttagga tcaatctgcc agccccattc acttgatgga agatacggat ttttaattcc
    38641 tccagttatt aagtttcctt ggaccccttc ccaattttct ggatgagcac tgactgcact
    38701 agacatgtag tagctaaaag aaacaaaatc aacagggtag gctgctaaga tttcttcatc
    38761 acctacttct atttttaagt tgacaccttt ttcttcaaac attcgttgcg tatacgctgg
    38821 ataatatcct cttgcttgaa catcactaaa gaacaaagaa gttctttgct gctcaattgt
    38881 tttttgaata tcatccggat cacaagaata cggaacaaga agctgatagc tgatcataca
    38941 accgacttgt gaacctttga taatttcatg acaagctttt gtagctaaag cacttgctat
    39001 aaactgatgg tgtgcacctt ggtatttcgc ttgttccaaa tggggatggt tttcttcaat
    39061 tactcctacg ctaacaaaag ggtgatgttt tacacagttg atctcattga aagtcatcca
    39121 atattttact ttatttttat agcgtttgaa tactgtttca gcgaaacgaa cgtaaaactg
    39181 aataagcttt cgattaggcc atccaccata ttctgtaacc aaatataacg gcatttcata
    39241 atgggaaaga gaaacgactg gctcaatccc atattttaga cattcgtcaa acacttgatc
    39301 ataaaaaaat aatcccgctt cattgggtgt ttcttcatca ccatgaggaa aaattcttgt
    39361 ccatgcgata gacatccgat agcatttaaa acccatttca gcaaataaac ggatgtcttc
    39421 tttaaagtga tgatagaaat catttccgtg tcttttagga taataatact tgctgttggg
    39481 atcctgcgcc gcttttactt gctgatgcgt catcatatgc cattgatctg tccatttttc
    39541 tttaggtaaa tcgctgtcga atgtatacac atcagacaca gataatcctt tttcatctac
    39601 atcccatgca ccttcacatt gattggccgc tgtcgctccg ccccataaaa atccttctgg
    39661 aaattttttc atatcgatcc ccctaaactt gctagttttc ttgtttgatt atttttctta
    39721 tgactagtaa tgatattttt gaatctacta ctatttgtaa caataaccat tgtggtcgga
    39781 tcatatccct ctttgattaa ttcttgcaaa tctacttcca tcaataaatc gcctttacgt
    39841 actttttttc catcagatac tgcactttga aagtatttgc catttaaatt cactgtatca
    39901 ataccgatat ggatcagtat ctctgctcct tcttcactga ctaccccaat tgcatgtttc
    39961 gttggaaaaa ctgttgaaac ttccccgttt acaggactgt agatttttcc gtccgttgga
    40021 atgatcgcaa acccttctcc taaagatcca ctagcaaatg ttggatcgtt tacttcagat
    40081 aaatcaatta tttcacctgc tgcaggagta cttaacacta tatcctttga ggattcttct
    40141 tcgatttcat ccttgtagcc gaaaagaaaa gttagcatac aggaactgat catcgctaag
    40201 aaataactag caactagcca aacaaatcct tcaccaatat aaatcgggaa agacaaaata
    40261 cttcttgtag caactgccaa agcaccggct cctgcggcac cagctactcc accaaatata
    40321 gctccacata tacaagcaat gtaaaatggt cgtttatatt taagagtcac accataaaca
    40381 gcaggttcag tgattccaaa taatgctgtg ataaatgcag ataaagataa actttttact
    40441 ttcttatttt tcgttttcaa aaatacccca agtactgctc ctgcttgcgc agctacggca
    40501 gtcatacatg ctggacctag cgtatcacga ccgtagaaag aaagattgtt ggtcattact
    40561 ggtacaagtc cccaatgtaa cccaaaaatc actaacactt gccaaaatcc gccgataaca
    40621 gcaccagata aaacagggct aaagttataa acagctaaat atccagctgc aatcagctga
    40681 cttgcataat ctgcaattgg acctacaact aataaagtaa ttgggaccat cacgatgata
    40741 caaatgaatg gtgtcaataa atttcgaata ctttcgtgca agcttttgcg aatcttaggc
    40801 tcaatataag aaagaaccca tacagctaaa attgctggaa taaccgtact tgtatacctt
    40861 gctagaatca cgggcatccc tagaaatcgc aaggttatgc tatcactata tgcactgaca
    40921 atcgtaggat aaacgagagc tgctgcaata accatagcga tgaaacgatc tgtattgaac
    40981 ttttttgcag cagtatatgc caaaaatata ggcaagaaat agaaaaatgc atcagctgct
    41041 gcattcaaaa tgatataagt tcctgattca gtagttaata aattcaaact cgtacataag
    41101 gcgagtactc ctttgatcaa accacttcct gccaagacgc ctaaagtcgg ggcaaaaaca
    41161 gcggcgatca tgtctataaa agagtttaaa atgccttttt tctcagttcc tctctttcca
    41221 ctagatgcat catcttctag tttcatttgt ttggataata cgtcatacac ttcatttact
    41281 gcatttccta ctacgacttg aaacattcct ccagcttcga ctacagtgat cacaccttct
    41341 agttgctcta ccttctcctt atctgccttc ttattatctt ttagggtaaa gcgtaacctt
    41401 gttgcacaat ggtaaacatt gctgacattt tcttcgcctc cgacaaatcg gacaatatct
    41461 ttcgctaatt gtgaatagtc catttttact tcccccttgt taattaatgc cgtagcaatc
    41521 cttcttacaa gataagtata gttcttagaa aacggtttct ccattcaatt agtacccctt
    41581 ataatagcga acgaaactgt acaatagtgt gtatggttta cttagccgag caaatcactc
    41641 actgcttgaa agaaaacaag tatatcgttt ctcttatagt caaaggagac aaaaaaaaca
    41701 cgcttgatct tcctttgctg atgtataagt ggtatctcaa gctgatactc tgtgattttt
    41761 gtttctactt ggcttttgat aagacagcac aacaaagtct cggcatttaa cggaagcata
    41821 actaggtcag aactaggaga gattagttgc tgaatggaat ctatcttttt atggattttt
    41881 atttgatttt cttctttgta gtaatgatca agcagttttg ttttcttttt ttcttcaact
    41941 atggtcagct tttcacgcaa aatctgataa atctcccaag attcaaactc atctagaaca
    42001 ctaggtagat aaatcaaatc atcccactca aaaataagct cttgctcagt agtcaaaatt
    42061 gcatcatatt ccgttatttt gtttgattga tttttgaaaa gatacactgg ttctatccgg
    42121 cattctttga tccacatgtt catactttgt tgaatctgtt tttttagtgt attcaaaata
    42181 ctaattggct ggttactaac aatcagtatc tccccttgtt ttcgaaaagg attcaacgct
    42241 tcggcaagat acaaaacaaa ataagcgctt tcagctaaat tgggacgtaa ttgaaaatgt
    42301 attgggaaga aacgccgaat taaataaatt tctaaaggat aacgggagat gatcttttgt
    42361 acaaaatgat tcatggtatt atgtccggca tccatcctcg ttttcatttg tttgatatgg
    42421 gttagcaaaa attctgtttc atggaacaat aacgaaacag gaagatctaa aaaacgaata
    42481 atcgtttgct caaatgctct tagattcttc tctatttctt tgtttttttc atcattaatc
    42541 atcaaataat ttaactctaa tagtcgattt tcaattgctt gtttttcttg attggaaaaa
    42601 gaatagggta gttctgatga taactttttt gtgagggtct ctaccatcgg aataaataat
    42661 gtatacctgt ttgttttttc taaaagttct tctcttcgaa gaatggtcaa aacgagatac
    42721 cgagaaaatc gtatgaagtc ctctcctgaa acaatataat tatatgatat aagaacctct
    42781 tttaatgtct tttggacagt cggcatgata ttggaaaaaa ttgtactgat ttgttgaggc
    42841 aaccttgcgt gttggatcac cgtttctgtt ccaacaagag ataagaaaat tctttttgaa
    42901 ttttcttcag caatgatttt tagtcctttt gtcgacgaaa cttccagttc taatttagga
    42961 ttgcgctcga tccaacgcaa aatcgttttt atttgtaatg atacggctgt ttttgaaaga
    43021 aagaaaatgt ctgctatctt ttgcatactg acataccctt tttgaaacaa taagtacgtc
    43081 acaatagttg aagctcggaa atccatgctg gaatagattt tatgtttcgt cacctcttgt
    43141 atgatttgct gtttagtttc agattcaatt gattctaaaa aataacctga acgtttattt
    43201 gactggataa tgattttctt ctcgtagata gcaagcatat ttttgatttc agtttggatc
    43261 gttttcttgt tggtacctaa agcttcagct aattcttcgc ttgttatcca atgattctta
    43321 tttcttaata ctacaagaat ttggagttgt cgtttattta agtccatttg cttacacctc
    43381 ctctttgtta taaacataca ggaaaaacct ctcaaatgac aatagtcact tgcagaggtt
    43441 taaaaatcat ttgaagaaag atctatattc aaggttttta ccattagact ggataatttg
    43501 tttataatat tcaaaactat cttttttcaa tctcctaaag cttccgctcc catcattttg
    43561 tctatccaca taaataaagc catatcgctt cctcatttcc cctgttcctg ccgatacgat
    43621 atcgattggt ccccaccata agtaaccaat gatttggcaa ccatctttta ttgcctcagc
    43681 aatttcctga agatgtattt ttagatactc acaacgtttt ggatcgtgga ttcgattcaa
    43741 ttcatctggt gcttcatcta gtccaatgcc attttcaact atgaataatg gtaattcata
    43801 acggtctgtc aattcattca tgacataacg aaaccctaga ggatcaattg gccaactcca
    43861 aggttcggga gatttttcct tcaaaaaggg attatcgaaa ccatttgctc cgccagtatc
    43921 tacgcgcatt tctgcttctt ttttatagac agcgctacga taataactaa atgcaatata
    43981 atctgccgtg ttagcttgca agcactgttc gtctccatct aacattactg gtgcacagtc
    44041 ttcttccttc catgtcctgt aaatataagc tggataatgc cctcggcaca tgacatcgga
    44101 gaaaaaccat gaattgcgtt taaattgcag tgaaccaaat acattctctg gatcacagtc
    44161 atatgcataa gtcgctaatg cagaaaagct acacataata cccatcttag catttggatc
    44221 gattttcttt aataattgag tagcacgggc gttcgctaga aacatatgat gacaggcttg
    44281 ataaatcact ttttttgtta gtgtagcaat gggttcaagt ttatctctag cagggaatgt
    44341 aaccaatcca gcagctgcat aaggcatacg aaaagcatta ttgatctcat taaaggtcat
    44401 ccaatatttc actttccctt ggtagcgttc aaagacggtc tgcgcataaa attcaaaaaa
    44461 gtcgattaat tttcgatttt gccacccccc atactctcct accaatgcta agggggtttc
    44521 ataatgacta agagtaatta ctggctcgat atcatatcga tttaatgtat tgattagtct
    44581 gtcataaaat acgagaccag cctcatttgg cttctgttcg tctcctcttg gaaatattcg
    44641 tgcccatgag atgcttgttc gaaaagcttt taaacctaac tccttcagta aacgaatatc
    44701 ctcttcaaat cgatgataaa aatcaatcgc ttcatgagaa agataatgga gtgattcgtc
    44761 taaagcaatc tcccaaatct ttttttcttc attccactga atactaggat gctggttcat
    44821 gatcccgata agcgtatctg tcacattagg taatttgccg tcttccaacc atgcaccttc
    44881 acattgatta gcagctactg ctccgcccca taaaaaattt tctggaaata tttcctgatc
    44941 cataactatc tctcctcgct atcaacaatt tcttacttga agtatagctc gattcatcaa
    45001 aacaagatat tctaactatt tcggcagaag agaagaaaaa aattgaatgc tactcttcat
    45061 ttttaatatt tatatataac aaatacataa gacgatcccc gaaagaaaac actcgttaac
    45121 catctaagca aacgatttat tttacttcca acacaaatgc ttcataaggt cgtaaatcaa
    45181 ctttgccgtc tggtaactgt ttcagaggat aatttgacac aataaccctt aaagcttcac
    45241 gtggaacagt gaacgtctgc ttttcactgg acatattaca aataacgagc cattgctggt
    45301 cttcatatat gcgagaatag gcaaagacat gctcaggcgt cttttccact aattcatagt
    45361 ctccccagac gatgatgtca tgttttctac gcaaatcgat caatttcttg tacatataaa
    45421 aaacagaatc ttgatctgct aacgcttctt ctgcattgat ttctttataa tttggattga
    45481 ctcgaagcca tggaattcca gtagtaaacc cagcatgttc acgggcgttc cattgcatag
    45541 gcgtcctagc attgtctctt cctttcgcat tgattgattc caagatttcc tcttttgtaa
    45601 atccagaaga aatcctttcg ttatacatat tgattgtctc aatatctttt gcttccgcga
    45661 tatcatcgat tgggcaattc gtcatgccta actcttctcc ttgatagata taaggggtgc
    45721 ctttcatcat atggagcaag atagcaaaca tcttggcact ttcttttcga tacacatcgt
    45781 catttcccca acgagaaaca attcgcggaa gatcgtgatt attccaaaat aaactattcc
    45841 atccttcatc acctaaagat gtttgccatt tggataatac ttttttcagt tcagagatac
    45901 tcaagggttt caaatcccat ttgtctttgc cttcttgctg atctaatcct atgtgttcga
    45961 attggaagac catggataat tcatttcttt taggattgga ataaagttta gcgatttcgg
    46021 tagttgctcc ccatgtttcg ccaacagtca tcagattttt gtcaccaaaa gttgcttgat
    46081 tcatttcctg aagatactca tgcagtttag gtccattact tgtgatttct ttatccggtt
    46141 ctttgccaat catatcgatc acgtccatac gaaagccccc aataccttta tcgatccaga
    46201 aattcatcat ttgataaatc tcttggcgaa cctgtttatt tttccaattc aaatcaggtt
    46261 gacgcttgct atacaagtgc agaaaatatt gccccgtttt ttcatccaac tgccaagcag
    46321 aaccactgaa tattgagcgc aatctattag gctcttttcc attgaccgga tcacgccaaa
    46381 tataatagtc ccgataagga ttttctttat ttttacgggc ctcaatgaac cacggatgtt
    46441 catcagaagt atgattcacc accagatcca tgatgatacg gatatttctt ttctttgctt
    46501 cttcaatcag ttcttccatt tcttccatcg taccgaattc tgccatgatt gcttgataat
    46561 tactgatatc atacccattg tcatcattcg gagactcgta tactggagaa agccaaattg
    46621 catcaatacc taaatacgcc agataatcca acctggaaat aatcccccgc aaatctccaa
    46681 taccatcttg atttgaatct tgaaaacttc ttggatagat ttgataaaca actgaatggt
    46741 gccaccattt ttgttccatg attttcctcc tataaatgga tgtttctatt ttttgcttaa
    46801 tcatttattt tagtctctct cactaaaaat tgtaacgctt tcaaatagct attgcacctt
    46861 gttatcggta acagaataaa acaaaaaaac agctgaggaa attccctcaa ctgttttttg
    46921 aaaggattga tttatgcagg gaagaatgct gatttcacaa cttctttata aacggtgttc
    46981 actcagctgt cccttggtat tagctcaaaa aacggcaaaa tatgaggaac tattttttcg
    47041 tttttttctc taatactcag gccagaacac tgatttcaca acctctttat aaacggtgtt
    47101 cactcagctg tccctcggta ttagctcaaa aaacggcaaa atataagaaa ctattttttc
    47161 gtttttttct ctaatactca ggccagaacg ctgatttcac aacctcttta tttcacattc
    47221 aatatccaca tgaatcccac aaatacaaag aacagaccat acatgattgg agatacttct
    47281 tttcctcttt tagctgctaa catggtaatt ggatagaaga taaaacctaa tgcaatccca
    47341 tctgaaatac tataagtcaa aggcatgccg atcaagatca agaatgaagg gatcgcaatc
    47401 tctaagtttc cccattcgat ttgtcgcaaa gattgtgcca tcaatactcc taccataatc
    47461 aatgctggtg cagttacttg tgaagtaaca actgatagta atggagaaaa gaacaagcct
    47521 aacaagaaga aaatccctgt agtaatagct gtgatacctg aacgtcctcc aacggcgata
    47581 ccagctgatg attctacaaa tgcaccaaca ggagaagtac caaataagga acccgcaagc
    47641 atagctgttg aatcagaagc taaagctttc cctacacgag gcatcttgtt gtctttcatg
    47701 aaacctgctt ggtttgctaa tccaactaaa gttcctgctg tatcaaagaa agtcacaagc
    47761 aagaatgtca gcactacgac ccacatttga agagaattga tgtcccctac atgttttaat
    47821 gcaactaaaa atgttggttt caaacttggt gctgcggcaa caatttcaga aggcattgga
    47881 atcaaacctg tgaccagacc taaaatcgtt gtagctgcca tcccgatgaa gatgccacct
    47941 ggtacacgcc gaacaagcaa gatagcagtt acgactaatc caaaaatcgt caaccaagta
    48001 gaaccaacgt tgagtgatcc taatcctact aatgtagatt cattcgaaac gatgatccca
    48061 ccttcgctca gaccaaggaa agcgataaat agaccgatac cgccagaaat cgcatatttc
    48121 aaatctgctg ggatcgcatc aatgatcatt tcccgcagct tgaagattgt gatcaaaata
    48181 aaaatcaaag aagcaacgaa tacgccggct aatgctgttt cccatggaat ccccattcct
    48241 aaacaaacag aataagcaaa aaaagcattg atcccaagag caggtgccgt tccaattgga
    48301 taacgggcta atacccccat taaaacacag ccgatcgcac tggctaatgc agtcgcagta
    48361 aacactgctc cttcatccat tccagaagca cctaatacag ttggatttac aaataagata
    48421 taagccattg agataaatgt tgtaaatcct gccagtacct cacgttttac cgtggtgttc
    48481 aatttttcta tttcgaagta ggaaagtatt ttttccttca tcttccatcc ccctaaatcc
    48541 gaacattata aataaagtcc gttaaaattt cgatttatta ataaaacaac gtgttttatc
    48601 atatactaaa acaaaacata agtaaacaaa aaattcggat tttaagcaaa atccgaacgt
    48661 tccatctgat taaatccctt tatctcaaag ttcatgaaca ttcactttgg attacaagaa
    48721 tttctttatt gtgttaataa agtttcttca ttctagtctc aaaaatcaat agtgtgattt
    48781 gattttacca tttcgtttct gttcttcttt gatgaattct tttgtatcga caggattcag
    48841 attatttagt ccgccaaaac ctgtatcgac catttttccc cagccttttg tcttcttttt
    48901 tcctgatttg agattctttg ctcgaagctt ctcactatgt tttgctcgcc gatacttcaa
    48961 ataaccttct aatcttccca ttagatccac tgtttttcct cctttttatt caatcagacg
    49021 actcagcttt tcctataaca aaataattgc cttcagggtc agaaaaattg aaagtcaaga
    49081 tgccattgtt atctgtgatt tctcctggtt cttccatgtt ctcgtagata gtttcaaatt
    49141 catccgtaaa aaatactaaa gaaggcggtg gtcctaatac ctctggtgaa aacttctgca
    49201 caaatacttt tgggaatacg cctaattcta attcttttga gatgctgagt acaatactct
    49261 taaattcttc tggcaattcg attgtttcag aaatttctgc accaattttt tctacaaaga
    49321 atcggcaaat catctctacg tcattgacat aaagcatcac acgtgttttt tgtatcatca
    49381 tttgaccctt ctttctattt gattttcttt cctgtctata aactgattcc attatatcac
    49441 gaaagcgcaa acaccttgtg tttgatacta ttcaagaaaa ataaagcaat agtttctagg
    49501 attgctttcc tccacaaact ttttttacaa aaaaaaaaac ggcaatgaat gccgctcttc
    49561 ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca
    49621 ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca
    49681 cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc
    49741 gtcagcattg ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca
    49801 tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg
    49861 aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct
    49921 tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga
    49981 ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa
    50041 cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg
    50101 ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat
    50161 aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat
    50221 aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt
    50281 ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg
    50341 gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa
    50401 aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt
    50461 cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt
    50521 tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc tcagattgac
    50581 tcgttcctgt atttagtttc aggtaacccg caggctcttg atcgatactc actaggaaaa
    50641 aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa gcttgtgtca
    50701 agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta aatgtttcga
    50761 tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat
    50821 tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt
    50881 cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag
    50941 aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga
    51001 tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca
    51061 aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat
    51121 ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta
    51181 ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag
    51241 aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc
    51301 tttattttgt ttgtaagact agtttacctc tttttgttgc aaatgcaact attttcaata
    51361 aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct tttacgaaaa
    51421 agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt
    51481 tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt tggaagtttc
    51541 attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa caagtttata
    51601 atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg ctcgcaactg
    51661 agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat tttcttcagg
    51721 agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc gtttatcttc
    51781 gattagtagg accttgccat cttttttgat ataggcacga acatctactt ttggtgttgg
    51841 atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt ttcctatttc
    51901 actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat caaatacatc
    51961 ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt gagccaactt
    52021 cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc aagtatagaa
    52081 gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa agaatgttaa
    52141 aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg gcgttcaaaa
    52201 gcttgcatct gcggtaataa gccccaccaa acgaaaaata tgacgagcta ttcttgtctg
    52261 gctgttctta ttaattgagg caggacagct ttttcacaac ctccccacta aacggtgttc
    52321 tcctaccaac tcctcggtca caagtcacaa tattcatcaa acatgagaag ctgttttttt
    52381 gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg gtaaacagcg
    52441 ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc attgatttct
    52501 ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt tacatcaaat
    52561 tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat ctttttgaat
    52621 gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc accatagtga
    52681 taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt gtaccgacgt
    52741 tgacataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata tccgtatcta
    52801 tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc ggacacaatc
    52861 actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg ataaactttc
    52921 gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc tgcatagtat
    52981 tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg tagtttattc
    53041 ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata ctttttcatc
    53101 tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa tcttatgctt
    53161 gacaatttct tttactgctt catttgctgg aatcacatta tggcgaagag acttgttgat
    53221 ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa gctccgttcc
    53281 aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt ctttcacacc
    53341 tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt tcagcaaggg
    53401 aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag caatagctaa
    53461 tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg tatatacctt
    53521 gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa gttctccttc
    53581 tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca ggatattttc
    53641 tttgaaaggc agatgggacc catcatacat gacagaagta tatccgctgt cgattgcttc
    53701 tttgatatca tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa ccatttcttc
    53761 ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt tagcggtatc
    53821 gacacttgtt tgaataatga tcggcgaacc catttctttt gctgcgccta gcatagcagg
    53881 aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact cttctgctgt
    53941 ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct ttttatttgt
    54001 ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg cccatgtagt
    54061 aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg ttatagaggc
    54121 tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaaactc ttttctaaat
    54181 attggatggc ttgatcgttc acatacaatt cagaaaattt aagtccaact tgttcataaa
    54241 gtttcgctaa tttatgctta tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct
    54301 ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt gccagaactt
    54361 ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct actttcaatt
    54421 aaaatgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg gctactgtat
    54481 ctacatcggt tgctgtagca ttgatgaatc ccatcgaatt aaagatcgtt actagcaaag
    54541 cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct cctcggcgtc
    54601 cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg gttagcatac
    54661 caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa
    54721 tcgttgtcgt aatgaaacct aaaatgacag catttgggct ataaggaaag aaaactggac
    54781 aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga aatgccggca
    54841 cgatttcacc taacagtaga cgtacaccag ctagcaatac atacacaccc actacgaatt
    54901 ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat tcagaagcga
    54961 atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg accatgacag
    55021 acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct tcgatcgact
    55081 ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat aagtacccga
    55141 tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga atgatcggtt
    55201 gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg accaagatca
    55261 atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt gccatccata
    55321 aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc aaaataatat
    55381 tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc aaatcattca
    55441 ttgcttgacc attgatagat tcaatagaag ggatgatccc ttgcatatga aatccttcag
    55501 taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaaccg gcactcaaca
    55561 cttgaaatcc taggagtgtc tttaacgtgc cagagatgac ttgtcctgct gatttcttct
    55621 gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga gtcaaaatat
    55681 tttcgatgat aaaattgata acgcccatgt ttttaccctc ttttcttttt gtatttagat
    55741 aactccctta tcttgcagaa ctggggtgat tttttcttca atttcatttt ttgaaacaat
    55801 atttttcaaa tagatcatcg ccgttttagg atcaactgcg aacttttcga actgcgaacg
    55861 aaaattttct gctgtgatga tcacatctgg tttcatggaa actgcggaag aaatatcaca
    55921 atggtccagt ttggcttcta ctcctaattt gttcaaaaca tcctcagcag acatctgtgc
    55981 tgcaaaacta cttcctaaac ctgcaccgca tacgaataaa atgttcaatt tgttcatctt
    56041 tgttctcccc cgtatcattt attatttctc aactactgaa aaaagcagtt gtttgaaatc
    56101 ttcgatattt gtgcattcaa tcaatcgatc caacttctct tcgttgttga tcaattcaat
    56161 tagttctttc atgatgttca gatgcgagta ggagtctact gctgccaaac aaaaaataat
    56221 ctttactgga tccatttcag gattaccgaa atcgattggt tgctcaatcg tagctacgct
    56281 gactcctaat ttattgaccc catcttctgg tcgggcatgg gctagtgcca tgtgtttgcc
    56341 aatcacgata tacggtccgt aatcattcac tgcacgaatc atcgcttcga catagcgctc
    56401 ttcgacaatg ttttctttaa ccaatggtct agcagctagt tcaatcgatt cctcccaagt
    56461 agtggctttt tctttgatca tgatattgct gtccgtcaaa atatctttta acataggctg
    56521 gatctccctt ttgttgattt ctaaattgtt tgtttcgaat aatttttcta acttgccata
    56581 gatgattcga tctactcggc caccactttc tttcatgaca tctattattt gagtgaataa
    56641 ctccgtcgat tcatgactgt taggctctaa ccgctgtagt tcttcatttc ttttcaaaaa
    56701 gtcttctata accaagatat tcgtctcagt taaaatcgga tcgacaacta aaagcggctt
    56761 atttcgataa ttcaattgaa aggtgctgaa aaccagattg acatccaatt tatctactaa
    56821 ttcgatttct tttgaactta aaactgcagt aatctcgatc tgaggaaact tttctttcag
    56881 attctctgct aacagatttc cagtagccat cccatggtta cagacaacga ctgatttata
    56941 aacataattc aagtctcgct tgattacact ggcgatcgtc gaaaagtgga tgacaagaaa
    57001 agctatctcg tcctccagta tcttgccacc aattacttct tcgatcgtag gaacaaaatg
    57061 agtgactgcg cggtagattg ggaaataatt tgttcggatg ttttctttca atggattcat
    57121 gacttggata tgattcttga ttcttacaat cagtgcagcc atgtgcttat agagattttc
    57181 gaataaagct tcttccttta gatggaaagg tacgtgtgtc tcgttctcta caaattggat
    57241 caattggatc gttagtaatt gagcttgtac ccactcgatt gaattggaga tatctctagc
    57301 attgaacgtc tcaatagtga aacgaagata atttctttct acttgcggga cattgtccaa
    57361 ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag ctactgtctt ttatttctgt
    57421 atttttccaa ttaatggcag agataaattc atgtttcctg attcgctgga tccagatcaa
    57481 tgtaaaaagc aggatttgtt ttttataaat atcatcttct tgtttcaaca acttattgga
    57541 atagatctcc tgtatttttt tgatttccac caaaggaaaa taatggagaa agattttttg
    57601 agccggtgtt aattgtgcat cttgattcat aaagaagtcg gtcattccca gattctgatt
    57661 gataaaatca tagatcatcg tacggataga tcgttctgaa cctttataaa ccagtccttg
    57721 ctttccataa ctgacgatct ctatcccata actgaccagt gttgctcgta accgtctcat
    57781 atcttcatct aatgtacttt tagagatttg aaattcttct tcttttttgt ttagatggat
    57841 cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa atacgttcgc gccgattcaa
    57901 aaagccgtct ttcacatctt ttatttcagt ttctaatcga gcttgctttt ccttttccag
    57961 cttcaacaaa aaaactttgt tgcgaattgt acggatctcc ggaagctttt tactgctcaa
    58021 ccagatattc gcttcaagaa cttcctgacg gatcgtccgc tgactgacat tgaactcact
    58081 tgctagatgt tttgctttta ctggatattg ggctgtcatg attctgtgaa gtatttcaat
    58141 tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa gtatatggtt ccgcttacat
    58201 aataaaaagt agatatttct gccacttgaa gtggaaagaa agtaagcaaa gcaaacaaaa
    58261 aataagccat atgctttttc atctcctcaa cgaaaaagac atacgcctat tattacaaaa
    58321 attttgccca tctatacaaa aaagaggttg tgaaaaagct gtcctgcatc aagtaataag
    58381 aacaactaaa ctaaaatagt gcttcctatt tttcgtttgg ttgttcttat tatcgcagat
    58441 gcaagctttt aaacaccatt tagtggagag gttgtgacag agatgttcag ccttgagcga
    58501 taagaagcga ttttgaaaaa tcgcttccca tattttacaa aatcgtgcct tattgccgat
    58561 aggctatctc tggcacactg tttattcaga aaaaggagct gcgacaaggc ttttgtcaca
    58621 actccgttca taaatatttc ctgtcaatcc agcataatcg gctgaataaa atcggcttgc
    58681 catctttttt ctttctcaga aaaatcaaaa taccatatct gagaaaaata ctgtcgttgt
    58741 gtcttacttc ctgagatcaa tcgcccatct tttcgatcga ctgaataatc acagcatctg
    58801 aaatcaattc ggatggcaaa actattttcg tggatctgtc gataatttcc tacttcctga
    58861 atagttattt ttttagtatg atttcttaca ccgtctgctt tgtttttttg cagaactttc
    58921 tgatgttcgg caaacagctt gtctgtataa cagcttctgg ctttttcagg agtttcttct
    58981 tcccaagcgg attggatctt catgaatatc ttttggattt ccagaaactg tgctttcttt
    59041 tctttagccg ttcctgacat gcgggacaat atctccgctt tcagcctttt cttttgtctt
    59101 ttattcttga cgatagaaaa agcagaaacc cctcctactg acaaaacagc taatgcaaga
    59161 aatcccgttt gctcataact agaaggacct gtacctggac cataataccc ataagaatct
    59221 ctgccgtaag aacgactgcc aaaataactg cccccatatg aacgaccacc aaatgaacct
    59281 ctgcccgaac ttcgaccgct agatgtacct ccaccatgac cacccgcgac agcgaatcct
    59341 tgggtcggca gagcaaacaa acataggccc actaacaaaa tgattgctaa ataattgatt
    59401 tttttcattg tcttcctcgt tatcttttat tacaaaaaac tatttttatg atagccttgc
    59461 taaaaggaag agtcaataac cagatgcata aactgttttt ttagcaattt tgtcttattt
    59521 agtcattgag taacatcgga caaatgtatg tgtcggcagt ccataattcc tctatcttca
    59581 gcatgaacaa gcttgcagac aagagatctg cgcttcctcc aggagagatt tggttttcct
    59641 gacaaaagtg caacagtgct gtaaatggac gatttggtaa agcactttga ctagcagcat
    59701 gagcaagttt ttgcatttcc actaacgttt tctgatcact gcgatgcaat acgcatgtgt
    59761 catctagcga actcatcaaa actaacaaca tatgcatttg tttctgtaca tatgaatcat
    59821 tatctagctc cttagctgct tggattgcaa gcctgatatg cggataccct aatatcgctt
    59881 cttcatatgc accatttacg ccatattttt gcttcaccct atgaccatga gtggtttttt
    59941 tatgacgata ttttgtatca ggaaaagcag caagcttgct gacatcatca agtattttcg
    60001 ataaagaaaa aggctgttcc atactgattt tttgtgcaat gacactgatc aatagcccca
    60061 ttgcccaaat cgctccttta tgggtattga tgccatttgt agcaatcagc attgcctttt
    60121 ccgcttttcg cccaatctca gcaattcttt ctcttaactc ctgatcaata gcatgatgcc
    60181 atgctatttg agccatttct tcaaaaaaag gagttagcgt cttagcagac ttaagaaata
    60241 aagcatagtc catatcggaa tgtgcacccg catccagtgc atccactaat ccaggttttg
    60301 gacttaatgt tacttctgcg atcaatgctt cctctaccaa cgcagcgagt tgctgagtca
    60361 ctgctttttt tcgttcgtca tttatcatga cactcactcc ttcttcctac tattacttgc
    60421 tgaaaccttt tttgatagct acccaaatga tatgaagtaa ccagcttaaa gcaaatgcac
    60481 aacttgccgt cattagataa gctacaagaa tcatgactcc tgaaggtaac tgcagccaac
    60541 tatctttaaa caataataac agtacttgga aaacaaatac attggctaaa tacgtacgat
    60601 aagcataaac agacaaccat ttgaacaatg gcaattttgg cgaatcgttt acaatcattt
    60661 tggaagcgcc tatgaagaca gcaaagatga tgactaaact gtataaactt tggatcgtat
    60721 tcaagtaagg tgcattcgca aagctcaagt ctcctggatg tgcgagcaat gaacggacag
    60781 aaaagaacgc gataactaag gcaacaggta agaaaagata acgtactctg tccaaatatt
    60841 gatagacttt ttcgtgataa acaaatgcgg ctaccccata aatcatgaaa ctagcaacaa
    60901 accgatctaa taagtaccag ctgtcatgat aaggcccagt caatacttgt gtttgataga
    60961 acagatacca tgcgacatat aaaatcgttg aaccaatcaa tactggccat acatattttt
    61021 tattagcaaa tactttgtaa ccgagccaaa caaaaaacgg catgaacaat tggatctgca
    61081 gcatcataac ggtataccat aaatgaggcg ctccatcacc tgtgacatat ttcagtaaaa
    61141 agcttgcagt atccgtatac ggagtttgtt gttggactgc tggaaatagc caaagataag
    61201 ccgtcgtcca tagaagatac ggaacgacta gttcaaaaaa cttttctttc aaaaattccc
    61261 aataagacaa atgctgactt gtttttacca tattataaac aattgcaaaa ataaacattg
    61321 gtgctgtgta tttaacgatg acgtaaaagg taccaagaaa agctgtattt gttgcgctgt
    61381 ggctgtttgc caaagcaaag ctcaatatcg tttgcatcat aacagccgtt acagctgata
    61441 ctttctggaa gtcacttacg ttgattttga tttttttgtt ttcaaacatc tctttctctc
    61501 tctttttctt tattaccaaa gttctttggt taatttaggt ccttcgactg ttttcaatag
    61561 aatttttttc tctggctctc tcaaatactc ttttagtgaa aaaccgcctt gccgagtcac
    61621 tacttgggta tcgaattgct caaaaaaagc aggatgcgat tgaattacct gaattggcaa
    61681 ttcaatcggt gaatttgcgt acagcagcaa atcaaaatca ctggtttctt ttactgccgg
    61741 aatcccagtc gataattcaa atcctaagct gccgccgatt ccccatttac atcctgataa
    61801 tatgtttcta gcagcatgaa attgctgata aacaggataa tccatgattt tctcttgtct
    61861 atacgattct tgttcgatga tgtcccacgg atgaatgact tgtttgacgg catgttttga
    61921 gatttctgtt gaccatctct gattgcgggc aggtccacgt aaaccaatcg gtagtatttc
    61981 tgcgtgatcc gtcgacgcac gacgaactgt tccgaacatc ttttcagtta gaacgaccca
    62041 ttcaggcaag gtgaaccctt ctatttcatg tttatcaaaa aggacaatat catgtgcttg
    62101 aatcattttt tatgcatccc attcttcgtc cattttggca cggactttat ttgtttcgac
    62161 acgtccgaat gttttggctg tttcgtttgt gtatcgattt gtcagcatcg tgtcatttgt
    62221 attgcggaca tcttgaatgg cttcattgat tgcttcgatg actttgctga cattttgttc
    62281 tgaagcagct tggtcggtga tatcttctag gaaatgatag atcgctccta gttttgtata
    62341 attattgata tcgtaagcga tagctggaac tttttcagca gctgcttcga tttcagcaac
    62401 cgaacgtttt gtcacacgtg cagcactttc tttagacatc gcttggacgg taatttcttt
    62461 attgtttaaa gcaatcaagc ggcttgattg taatccatgt gctaaaaatg cacctgaaat
    62521 agcatttcct gcaatcaatc caataactgg atgtccagct tgacggagtt ttgcataagc
    62581 agcagcacta ttagccaaag ccacatggat accgatcaat tcttcgttat agccgtatgc
    62641 ttggcttggt acatcgatca caacgatgat tggccgtttc acttttttgt ctttatcttc
    62701 ttcgtaaacg tgatccaaaa tttgtcccat gcggaaacct tcaactaaac caacttcacc
    62761 gtttcgtaca cggtacatcg ggtttttagg atcaggtacg attgatgtca cgatggcttc
    62821 ttctatgaat ttacttgatt taccataata cacagtacta atagaagaag ttggattttt
    62881 catgccagtt agtgccgcaa accaatcgta accaacactt gctgcagctg gttcatttcc
    62941 ttctgctact tctggtaagt caattgattt tgcttgatgg ttagcaattg cttcattgta
    63001 atcgtcaatg gccattggtt ttgaaagatc taaagaatca agtaatgata aataaaatgc
    63061 tgagctttca ctgcgatgcg tgtcttttcg ttcgtccatt gctgcgatga cagcagcttt
    63121 gattgcttct acactatcag tcactaattc atcaatgatt tttgattcta cccgttgacg
    63181 agatcctaat gtattccaaa tcaagtcttt gtcactagaa tcaaattctc gaacgcctgc
    63241 ttcttgttca ataacttctg gtccattcaa accgactctc gctttttttg ttgcgattaa
    63301 ataactcatt aatgctgaag taatcgacat accgccaaat gatcctacac gtcctggaat
    63361 caaaccgatg actggtacat attgttttag agcaatgatt acatttccga tttctgaaat
    63421 agataacagt ccgtagtttg cttcttgcaa acgaacacca cctgtatcta gtacaatgac
    63481 tggaaagatc tcatttcctt ttttattttc ttctaacaca tgttcaagtg cagcaacgat
    63541 ttttgcaccg gaaacttcac cgataccgcc accttggaat tttccttcca tagcgattac
    63601 tactgcttgt ttgccattga tcgtgccacg gctgacaacg atcccgtcat cactttcagg
    63661 gacgattcct tgtgctggta aacttggcgc gatgatttga tcgagtggat caatcaattc
    63721 acgtccattg tcaagtagag cgttcactcg ttctctggcg tttaattcta caaaactatt
    63781 tttcatctga aagttcctcc attgcttgcg tcaatcgtag gtacacaact cctggtgtag
    63841 caccgaagtc attgatcacg atatctgctg aaattggata acgatcaaaa aatctcgtta
    63901 atacattgtt ccatacttcg ccgaagccat cgcttcctgt acaaattttt acagtagatt
    63961 ctgttttttc tgttggaaac atcaagattt ccaaatctcc tgaaccgacc actcctacat
    64021 ggattttctt attgatcggc tgtttcgttg gatatttata agctaattgt tccatggatc
    64081 tctccccttt ttgtaaaaca gagcatacac gttctgctta ttcgtctttc ttcttccatc
    64141 tgggtaaatg gcgacaaaag gtttttctcg ccaattttat gaccaagttc tgaatttcat
    64201 tggtggttca tacaggccat ctgaccattc gatcaaatca tcgatggttt gtgcagctaa
    64261 taatgaacgt ttcgcttcac tacgtttgat gcctagatct tctggtaatt tcacgatgcc
    64321 ggctgcacgc aagctatcta gtgattcttt cgtgctagtc attccaagag gtgtaacacc
    64381 gccaattgct gcaattgctg cttgtcgttc ttcttttgtt tttgctttgt acaaataagc
    64441 aattccttct tctgtcacga tatgtgtagt atcttcgcta taaatcatca ctggtgcatt
    64501 ggctaagcct gcttgttttt gaacagctag tgcatctaat tcttcgacaa agactggttt
    64561 tttatttgaa ccatatgttt caaccatttg aacaactaat ttttggcctt tccctaatgg
    64621 atcatcattg tcgcgaagtg attgccatgc tggtgtagaa tgacgacgac cgccaggatt
    64681 atgtcccatg ttaggcgcac cgccaaaacc agataaacgt ccttttgtta cggtagaaga
    64741 gtttccttcg taatctaatt gcaaggttga accgataaac atatcgatgg catattgtcc
    64801 tgcagcttgt gataatgttc ggttactgcg catcgtgccg tctttaccga caaaaaagac
    64861 atctgggcga gcagcaatat atttttccat accaacttcg ccaccaaaac tatggatgct
    64921 ttcaacccaa cctgattcga tggctggaat catcgttgga tgtgggttca atacccagtt
    64981 cttcgcgatt ttccctttta accctagtga ttcaccataa gttggcagta acaattcgat
    65041 agctgctgta ttgaatccaa taccgtgatt caaagattgt acgccgtgtt ctgcgtagat
    65101 tcctttgatt gccatcattg ccattagaat ttgcaattct gtgatgtttt gaggatctcg
    65161 tgtgaataaa gcttctaatt gataaggttt atctgctttc acgacaacgt ctacccagtc
    65221 accagggata tctacacgtg gtaaagaatc gacaacttcg ttgacttgaa cgatcacgat
    65281 accatctttg aaagctgcag cttcgatgat tgttggtgtt tcttccgtat tgaaacctgt
    65341 gtacaagttg ccgtctttat ctgctttatc tgcagctact aaaacaacat ttggaatcaa
    65401 atcgatgaat aagcgtccat aaagttccaa ataagtatgg atatcaccta atttcattgt
    65461 gccgtcttca atcatttggg cgacacgcaa gctttgtgca ccagcataag agaaatcgat
    65521 cttgcttgcg attccttttt caaaaatatt caaatgttct ggtcgtgaga tactagacat
    65581 gatcaaatgc agatcattca ctttttctgc atctactttt tcaagacttt ctgaaagaaa
    65641 acttgcttgt ttttgattgt cgccttctaa tacaactttg tcgccgggag caatcagttt
    65701 ttccattact tctacgattt tttcagtttc tacatacttg ccattgagca agttgctgat
    65761 tttttccaat cgctgcgctt ttttttcgcg tttagaagcc cagttgcggt ttggtttttc
    65821 cataattagt catctccatt tttgatatag aaacgtggcg tcatcaatac catgttggtc
    65881 tagattgatc gtacggatcc ctttatctac accaaatttt gtatgaatca gctttgtcaa
    65941 agtattgccg gggggaaatt caatcgtcag atccattcct aattcttttg ccaagtccat
    66001 catttggttc cactgcacag ggaaagctag gttttccaac aaatctttac gaatctcttc
    66061 tgcgtcctga gtggctcttc catccgtatt ttttatatac aaacatctag catcagatag
    66121 atggacagac tgcatatacg gcttgaattt cttgaccgta tcattcatca agagacagtg
    66181 agatggcaca gggactttca gcagcttcgc tgtttgcgct tggtgccgtt tggcttctgc
    66241 caacgtaacg gataaacctt cgatactccc agataacgta tgttgcatcg gacaattttc
    66301 atttgacaga taaactggat ggtcaggatc aaatgttttt gctacaattt cctcagcttc
    66361 gcttctcgtc acacctacta ctacgcccat tgcataaccg cttggataag cttctttcat
    66421 taagactgcg cggtgataga ctagttccgc tgcattctca aatctcaaag atcgacaagc
    66481 aacagctgca caaaaagcac caatcgaatg tcctgctgca atatcaggct gaataccgtt
    66541 tttttccatt tcatctaaat aaaatactgc ttttgtcagt aatgctagct ggataaaaac
    66601 cgtatcttga tagttttctt ctttatcaac taaccgatac cccgtcacat tttctaaacg
    66661 ttccatgtac atttcaggga tatccgctaa catccctaac ttctgactgc cttgtccagg
    66721 aaataagaaa cctgtactca tgagcctcgc ctcctcagca cttacttttt ccactgctat
    66781 cttagtcaat ttctgctata ataaaaaatg ccaaaaaaag aaaaaaccat aacaaaaagg
    66841 tatggatggg atggataatt atgaaattgc ttcatctgca gtattttaaa aaagtattcg
    66901 aaacaaaaaa tgttacccaa gcagccaaag aattatttat ttctcaacct gccttgagcc
    66961 gtgcgatcaa acatttggaa aacgaattag gtgtcccttt gttttaccat agcggcagaa
    67021 acattgagac gaccgtttat gcagaagaat tttatccata tgcagtcaag acattagaca
    67081 cattagaaga aggaatcaaa ttattgtctt cggtcaatga gaagatcgtt acttccgtca
    67141 tattgtattt agaggtagcc tctgtttcta ttccgaactt ggtccgaatt tttatggaaa
    67201 agcatcctga tatccaatta acgatcatgc aacatcattt accagatgat attaccgatc
    67261 ctgtactgta catcacttcg gagatgaagc ctggcatgac caatattcct atcatgaaag
    67321 aacctgttta tgtcgccatt cctaagaaac atcctcttgc taaaaaagaa catctgcaat
    67381 tagaagatat ccaacgcacc cccatcttga tgctgtcaaa gagcaacgcc ttccgtcata
    67441 cactggatgc agcacttgaa gcgaaaagta tcgaattgac gatcagttcg acgacagatg
    67501 atcctgcgac attacgttct attgtcaagc aaggattagc tgttagcttt ttcccaaaag
    67561 tttcttggtc ttacgataaa aatgatcctt tcgtcttgcg ggaaatcaaa gatttgccct
    67621 tgacacgaac aatttattta tcttctagct tcacagaaga tcatcctttg atcaaaatga
    67681 ttgcaaaaac gatgcgggaa ttttatttag gaaaataaaa aaaaaagagc tgggacaaaa
    67741 ttcttgtcgc agctcttttt tcgaacaaac ggtgttccag aagtaatttc ttagaaaata
    67801 agccgaaatt tcccaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
    67861 gctaaacatt tctgtcacaa cctcttctct attgtcctag acattcattg cgttgattta
    67921 aaacttccat caatcaaatc tgctagttca tctagctttt ctattttacg tagatcaata
    67981 gagatccaat gttctttatt catgtgatag gctggaaaat acccctcttt tcctcgcaaa
    68041 attccgccaa gttcaggatc gaccttcaaa tcaagaatat tgatgatcct atggtcactt
    68101 gcatcatcat agattttttc ttctggaaca gacatcagga ccccaaacca ttttttgctt
    68161 ttttgatgtc tgaatacgca ataatcagga aattttttaa aaatatattc tggagacaaa
    68221 ttgtatgttt cattgatgtg atcgattagt tcttttttcg ttagcatctg cttccatctc
    68281 tttcttattg attcgcttta ttcaaagata ccacgtaaga atagaaaaat gaatacaagt
    68341 agttgctaat caaatacttc gatatttttc ttttttatca actcttaaat gttcaatccg
    68401 cctgtgttgg tcttgctacc atctactatt aaaatgttta ttattgtttg cccacctatt
    68461 ttttgatttt agtggtatca tttttcaagt tcatgatgaa attcttatca tcagtaaatg
    68521 ctccagaaaa ataaacaatg attgaaaaat cagttgaaac ttgaccaccg tttattttct
    68581 ccgtattctt agaatagaag ggagtctgat tgatgtattc actaatgaaa aaaatcatta
    68641 ccgaaaaaga tattcgacgt catgtttctt tagtagaaca attgctgaat catacaaaga
    68701 taaccgtaaa tgaattagct gaaatcatag ggacaaccga acgaacgatt ttttctgacc
    68761 ttcaatctat tcgctctcag ctgcccgaag gctgggacat catcagcgat caagcaggaa
    68821 tcagtttgca aaaccagcaa aatttattaa caaatgacct ttgggagatt tttttcaaac
    68881 aatcagtcag tgtcgaactg ctgaaaaatc tgctttttac aaaaaaagtt gctgtccctg
    68941 attttctagc cgaccatggg ctttcttatg ggacattgaa aagacatgtg actaaaatca
    69001 atcagcgttt agcaagttac gacttgcaga tcgatttgac gaaatataca gcttgtattc
    69061 ttggaaaaga gcgtgcgatt cgtacttttt atcatcgatt gctgattcct ttcactcata
    69121 acaactattt tttcgaggat tattcgatcc atgaatccca ttattttcaa tttttaagaa
    69181 acctcagtca aactgaacta gcggttgaaa cagaagagat tttcggaaca tgttggttct
    69241 ttatcaatac gatccgtata aaagcgaatt gccgattaga ttcttctatc cataccaaca
    69301 gcacactctc ctccctgtat gactctgcat tgaaaaaatt gtatttaacg gaaggtattt
    69361 atcttaaaga cactgagctt tcttttgctt ctttctgctt tctggaaagt tggaattaca
    69421 acaataacta tggacaagaa accgctcgct gcctgcatca ttctccattt ttagaggtcc
    69481 tcgaaacgtt tgtagaggaa ctagcttcag agctgtctct tgatcaacta aaaaaaacat
    69541 ctctgactga taacttggct ctcctcatat taaaatatca tgaatcgccg atattgattg
    69601 aagcattgga ccgtcaatac catcgttttt tagaaacata tgatcagcaa tatgcacact
    69661 tgtatcaaca gaaagagaat ttactgaacg atctaaaaaa agaaatagaa atcaatgaac
    69721 caaattactt tctccaactc ttgtctctac tgatccaaca aacgattttt tctatcaaac
    69781 cgaatttgtt caatgtctat tttattttcc aaggagaacc ttcatggaag gcgttcttgc
    69841 agcaggaatt aaaagattac ttaggaaaac gtgtctgctt cttacctatc gaactaaccg
    69901 atctttccac tatttctttt gaaaaaacag atatcttgat ttccaatttc cctctagatt
    69961 ctctagatat ccctattgta tacatttctt ctattccaac gaaaaatgag ctcaatcgtt
    70021 tgactgaact cactttcaaa acttttttat agtaacggct agcgactgta ttctctagct
    70081 gttttttctt ttttgcgaaa cagtttgttc atgatccctt tttctatttc tacaactaag
    70141 aatagaagaa tcacatttgc tccaatcaat aactgttgtg tcatcgttaa acttactgta
    70201 ccgatcagct gctggccgac tggtagaaaa ataaccatcc cttgcaataa agcaaggata
    70261 cctaatgaaa gaaaaagcgc tttgttttgc aaaagacctt ggttcaatga cggcttggac
    70321 aactctctgc aattgatcat gtaaacagct tgagctaaga cgatattttg cagaagaatg
    70381 gtttgctgta agctttgccc ttcaaactgc atagccaata aataggcagg aaccatgatc
    70441 agtagggaga catagatgat ccgaaacaaa tgataaccag ataggattcc ttgctttgga
    70501 tctcttggtg ggcgcttcat gatttctgga tctggcgact cgaacgataa agtgattgtc
    70561 gttaccatat tgacccataa aatctgtact ggggacaaag gtagcgggcg attcagcata
    70621 agtgcccaaa tcaagatcaa tccttgagct aatgcagttg gcaagaagaa attgatggtt
    70681 ttttgcaaat tatccaaaat ccgccggccc tcttttacgg cttttgcaat cgtatggaaa
    70741 ttatcatctc ctaaaaccat atcagctgcc tgtttgctta cttcactacc tttgattccc
    70801 atagcaattc ctacatcgga tcttttcaaa gcaggtgcat catttacgcc gtctccggtc
    70861 attccgacga tttctccatt tttctgcaaa gctttaacaa ttcgcagttt atgttctggt
    70921 gtcgttcgag caaagacatc gactattggc acttgctgaa tcaactcttc atcagacata
    70981 agatccaaat cgatcccttc taaaactttg gcagtatgtt ttaagccaat ttgttccgca
    71041 atcgcttttg ctgtgtcttt gtgatcccct gtaatcattt tcactgagat ccctgcttgt
    71101 tggcattcct ttacagcttt gactgcactt tcttttggcg gatcgatcaa tccggcgatc
    71161 cctgcaaaag ttaattgggt taaagtctca tgcgtgagtt cctgtttcga atctactttt
    71221 ttataagcaa atcctaaaac acgctgacct tttctcgcaa attctgcagc tgtctgactc
    71281 catgcttctt gttctgcaga agaaagagtt gatttagcaa ataatacttc tggtgcacct
    71341 ttgacgtaaa tcaccgctcc tttttcctcc gcatgcatcg ttgccatata tttgtattca
    71401 gaactgaacg gaatctttgc ttctattttt ttcaaaggta gattggctgt ttctgtgtgt
    71461 cgaatcagcg caacttctgt cggattgcca cttaaatttt ctgttttctg ttcttctttt
    71521 gtttttaatt cctgacagtt tttcataatg gacaacacgc aggcttcttc ttttgcagct
    71581 acatccacaa ctgtcatttc attttttgtc aatgttcctg tcttatctga acagatcact
    71641 gtcatagaac ctaatgtttc taccgaagga agtcctttga tgatcgcctt ttcttttgcc
    71701 atttcatgta ctccaacgga tagaatcatg gtcaaaacag ctggcaatcc ttcgggtacc
    71761 attgacacca ccaaagcaat cgtagccgaa agaaggatat gccaatccat accatatcga
    71821 aatgtcgtaa aaataactaa gaataggctc aaacaaagaa tgccttgaaa aatttgtttg
    71881 tttaatcggt tgattttttt aatcagaggc gtcgtttgtt gttcgaccga ttgcaatgct
    71941 tgattgattt taccgatttc tgtcgtgttt cctgtttcta caacgacacc gactgcagaa
    72001 ccagattgta caagcgttcc tgaaaaagcc atatttttct ggtcgccgat cgattcttct
    72061 tctgtcaaaa cggcatgact tttttcagat gcttctgatt ctcccgtcaa aatcgattct
    72121 tccactaata aatcatgtgc ttcgattaat cggatatctg ctggaacgac atctccggca
    72181 cgtaagacaa tgacatctcc ttttaccagt tctttcgcag ggattttttc ttgcatcccg
    72241 tttgatagta ctatcgcttc ttgtcccatc agctgcttca aaccattcaa tgattcttcc
    72301 gcttttcttt cttgccagta actaacgagt ccattgacga tcactacgaa taaaataata
    72361 cttccttcaa tatattcact agaagcaaat ttcaaaaaag aggcaatgat caacacgatc
    72421 atcagcagat cagtaaaatg ctttgctatt tttttccatc gttttgtctg ctgccttgct
    72481 tttatttcat tatatccttg atcagtcagg cgtttctgtc gttcttcttg cgacaagcct
    72541 tctgttgatg tttgatagtt tttcaataca tcttctaccg tcaatctgta ccagttcatc
    72601 ctatcccctc ctgtttaatt gatactgtta ttatacgcag ggaagttttt tcaggcgtat
    72661 cgattatttc aggggggtct gaaataattc gttttgcaaa atgtatctgg gcctaactga
    72721 tatttctatt gcttattctt ttatatacaa aatttaagaa tacaaaccag ataaccaata
    72781 aaaaaggaat aaagcgtgtc gtgctttcga ataacaagct gaaagcaatt gaagtaaaaa
    72841 acaccaacaa agcaagatct ttatatcttt gtgtagtgtt ccgtttcttt tctttaataa
    72901 attgaacatg tgcacacaag atcgaagacc acacaaatag aaaacaactc gttgtcatgc
    72961 ctgccaaaag ctgaaaaaac aatcgactat cagctacaaa aaaatgtaga caaagaccgg
    73021 caaaaaataa cattcctgta attaaaatac cgtgtaaagg tacgcctttc ttagaaagcg
    73081 cacaaaacca atggggcaaa cttttttctt ttgctgtttg atataaaatc ctgctagtac
    73141 tgtagactgc actatttgct gaggacaaag aagcaaccaa aatcattaga ttgacaattg
    73201 ctgctccgcc ttggcaatgg atcacttgca gcatagtcac taaaggactt tcttttaaat
    73261 ctaaacaatg ccatggaaca acaaataaaa tcgacaaaat cgttcctata tagaaaaaga
    73321 gaatacgcca aggcagacag ttgattgctt ttttcaaatt cttttgcgga tcacttgtct
    73381 cgccaacggt taagccgatc atttcaattc cagcaaaact gaataaaacc ataggaaatg
    73441 agactaccat tccagcaaag ccaaacggta gtaattcatc gatctgtcca aaattttctt
    73501 tgattccgat agactgggta tgaaaatctg agacactcaa tcctataccc aataagataa
    73561 aaaggaaaat ccccaagatc ttgatgattg agaaaaaaga ttccaattca ccaaaccact
    73621 tgactgcaac aaggttgact gccattaaga tcattagtgc gctacctccg cttatcaaag
    73681 ctggtacatg tacaaaccag aaatggatat acaatcctat tgctgttaat tccgcaagtc
    73741 cagctccaag ccagcaaagc caataagtcc aattgacaaa aaatgcccac tttttgccta
    73801 aataatagtc aacgaaatca ctgatagagt taaatgtact atttgctaaa aataaatcac
    73861 ccatggcccg catcaaacca taaatagcca aaccgcatat caaataaacg agaataacag
    73921 ctggccctgc tgatgcaatt gcctttgctg aacctaaaaa taatcctgtt ccaattgcgc
    73981 cacctaatgc gattaactgg atatttcgat tagttagagt ccgtttcatt gccggttttt
    74041 ccatcaagtt cacctttttc tttttcaagc agcatggcga cttcctgttc ccaagaaggt
    74101 ttctttattt tttcaataca gagtggtact acaaacaaaa tgacactgat ggctgtgact
    74161 gcaattagat tgatccatgt tccagctttc atcgtcagtc cgacagaaaa tccgattgcc
    74221 gcaaataaaa caataacgac agtccaaact ccaagattgc tctttttctc tttttttcca
    74281 attctaaatg gtcgattcaa ctttgcatgt gtataacgca atctcaaaac tgcgatcccc
    74341 atgatgacat agacgataca gtagataaca gtagttgaat ttacgagcat caaaaatgct
    74401 tgattgacgc caggaatcag caaataaaaa agtgcaaaga ttgagataat gacagactga
    74461 gtcaaaataa cggatttgga tacgtcaaat tgattcgtct tccaaaaatt gaattttggt
    74521 ggatacgcac cttttctagc actttctgtc actgtctttg ctggtcctga ggcccaagca
    74581 gataattgga ctgctacacc aatgaagaca agtaaactaa acagattcac aataagatgt
    74641 ggcaaaccaa gaatttggca ataaatcgaa atagattgcg cgatattatt caattccatt
    74701 tgtccttttg gtacgacatt tgctacaact aaagcattca aaatattgac gaaaaatata
    74761 aaaattaacg cggcaaatac tccgcgaata tacgttttaa ctggattttc caatcgagtg
    74821 atatacacag aggacatttc gataccagta aaaataaaca tgatcggcgc aaaataaaca
    74881 aatgaggatg cagtcgtagt atctggtatt aatttgtccc atgaaaaact gcctaatgtt
    74941 ccggttggta cgatcccaac tttgatccac gcagcaaacc ctaataagag catcatcgtc
    75001 aaaggcacgt acaatcctaa ccaaacaccg acttttccgc cgatttttgc catatcaaat
    75061 ttcaaattca aaaaagtgat gatccaataa acgactaaaa tgacaactaa tgtaaatttg
    75121 ctgttcaaac caagcggcac attatcgata atattaccaa acaacggagc gagtgctgaa
    75181 gcgaccatca ccatcccagg aaacatttgg acccataaca gccatgagac aacgaacccc
    75241 catttttttc ctagagcatt cgatacccaa agttctggac cacctttttg gggaaacatt
    75301 ccagcaaatt caccagagat caatgagatt ggaaaagcgt acaataatac tgcaaccagc
    75361 atgtaaaaaa tcatcgtcca gctcgttgct gctacatctg gaatatttcg tacacttgct
    75421 acaagggcgc aggtcattcc gataaaggtc atggtcgtga tttgctttga tgaaccagta
    75481 tctttcatct ttattccttc tttcaatagc gacatatgtt tttgttttct tttatttttg
    75541 ttcaagaaca gaaatgacaa ggtttcatcg attcagtaca aaacccagta tcaccgaaac
    75601 atccattgaa cgatcttagt gaactgggtt tgatacatga aacgaacgat ccaacttata
    75661 gaacgtttta taaaaaagaa tatcttggcg aggaatatat gaaaaacagg gggaaataca
    75721 attgttttga tgaagcaaat aattgtctgt tctccatatg tctcttgtta ttctttcaca
    75781 caaaaatcag ttctattgga tgatttcgtt tagtttcttt tccattgctt tggcgatttt
    75841 ttttgtataa aaattgaaaa ttcgatcatc attcaaataa ggagtcatga tagccgcacg
    75901 tagcagagtg attttctgtt cttttttcca ttcttctttc gttagaccca ttctttcaac
    75961 aaaacgaatc ggagaatcac cgtaatcttc ttgtgtaaat gtagtatgag aagtaataaa
    76021 ccgttcacca taaacatcgc catccatata agaagaccga tcgaacattt tttcatttaa
    76081 ttcattgata gcgttcaaat ctgtacagcc ttgttctttg aatacccaat tgaccatatt
    76141 aaaatcagga tgattcaatg gatagacttc gattgttttt cctttaacag taaaagtcag
    76201 atgatcgaga aattctctga atctttgagc tgcctcgatt gaagccccaa tcaattgacc
    76261 atatccggtc acattcaatg gcaatacacg atgtgctgtc cagactgcgg cggcggttgc
    76321 tccagctttt gatccttcca aaatataagc tcctaacata tctggagcct ttactgattt
    76381 ttcaaacaca tatggcgcga aataagaaat gatatttctc atatttttat gtttgattac
    76441 gattcctcca gcagcataag ggacatagcc catcttatga ggatctatcg tgacagaatc
    76501 cgcttctgaa atagctctga acccttcata tacctctttg tcaatcgtta cgcagtggtg
    76561 gaaaacatga tgttcttcaa agaattcagc taaggaagca tacggcacaa attctcctgc
    76621 ttcgtttaga aacagtgaac gcgcatagcc gccatatgcg gcatcaacat gtaaatagaa
    76681 atagatccct tcatctttta acctttctct taattggacg attttatcta cactgtctac
    76741 ttgaccttct tctgtcgttc ctacgacagc aaccacacct agaattggga ttttttgtcc
    76801 agctaattca cgaattgttt tttccaaagc attaatatcc attcgataat cctcttgaac
    76861 tgggatagca accatctgat ctaagccgac tccgcaaata tctaacgcct tcatccaaga
    76921 atagtgtttt gtttgaggga ccagccattt ccctaatctt tggatatttt ttccgctacg
    76981 ggaagaagct gctttgacct catccatttc ttcgtccgta aagctttcgg tcatttccaa
    77041 aatttcttca acagataaat tcaaaagttc ccactcagac atttttttca ctttttcagg
    77101 cagcacttcc ttgacagcta aaggaatcga tttgatgcaa cgggcgtacc ataatccttc
    77161 aagattagca atagaaccat ctgctgcaat gtgtccccat ccgtctgtca tattgaataa
    77221 tgaagcaaaa tcctgtccta cttctgcttc catttgcgaa gttgccatag aagattccaa
    77281 tgcaacatta tttgggttcc acagcatcgc atagttgtaa gctaataaag caggcatcaa
    77341 tgtttcagca ttcatttgtc cccagtaacg gcctgctgaa tgccatggga tcgatcctgc
    77401 gcgtaatcgc tgactgactt catccaagac ctctatcata tggtcgcggg tggctagata
    77461 atctggagtg agcttatctc cttcgctaat tgcaggcata tctgaaggaa tataattttc
    77521 tctccatcca agatgttcat ctaccatctt gttcaacaac attttataaa ctggtccatt
    77581 ttcagctttg tcgcctataa agacggcctt gatatccata tctttcattt ttcttccctc
    77641 catattttgt tggtcaatgt cttgcagata catgtgttta tctgtaacac ctcttcacta
    77701 tagcgccaaa aatgagaaat ttttttttat aacatcaaat ccacgtagat ttttacaaac
    77761 aaaaataaat aaagtcggat ttttctgata attcaacgca aaaaagcgaa caatttttca
    77821 ataaatcagt cttctaaaat agataaccta aaaaaagcga acaaacaaat aaaatgactg
    77881 tccttcattc actaaa
//
LOCUS       DACDYJ010000004        72823 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDYJ010000004 DACDYJ010000000
VERSION     DACDYJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.004"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(159..443)
                     /locus_tag="IWM51_000260"
     CDS             complement(159..443)
                     /locus_tag="IWM51_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969708.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(461..1351)
                     /locus_tag="IWM51_000261"
     CDS             complement(461..1351)
                     /locus_tag="IWM51_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP9969709.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1371..1589)
                     /locus_tag="IWM51_000262"
     CDS             complement(1371..1589)
                     /locus_tag="IWM51_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969710.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1559..3259)
                     /locus_tag="IWM51_000263"
     CDS             complement(1559..3259)
                     /locus_tag="IWM51_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP9969711.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3526..4299
                     /locus_tag="IWM51_000264"
     CDS             3526..4299
                     /locus_tag="IWM51_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969712.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4380..4949)
                     /gene="thiT"
                     /locus_tag="IWM51_000265"
     CDS             complement(4380..4949)
                     /gene="thiT"
                     /locus_tag="IWM51_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP9969713.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5015..5117)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5123..6094)
                     /locus_tag="IWM51_000266"
     CDS             complement(5123..6094)
                     /locus_tag="IWM51_000266"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9969714.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6188..7561)
                     /gene="glmU"
                     /locus_tag="IWM51_000267"
     CDS             complement(6188..7561)
                     /gene="glmU"
                     /locus_tag="IWM51_000267"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP9969715.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8064..8882)
                     /gene="purR"
                     /locus_tag="IWM51_000268"
     CDS             complement(8064..8882)
                     /gene="purR"
                     /locus_tag="IWM51_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP9969716.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9049..9873)
                     /locus_tag="IWM51_000269"
     CDS             complement(9049..9873)
                     /locus_tag="IWM51_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9969717.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9846..10526)
                     /locus_tag="IWM51_000270"
     CDS             complement(9846..10526)
                     /locus_tag="IWM51_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9969718.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10549..11487)
                     /locus_tag="IWM51_000271"
     CDS             complement(10549..11487)
                     /locus_tag="IWM51_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP9969719.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11732..12583)
                     /locus_tag="IWM51_000272"
     CDS             complement(11732..12583)
                     /locus_tag="IWM51_000272"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP9969720.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12737..15310)
                     /gene="mprF"
                     /locus_tag="IWM51_000273"
     CDS             complement(12737..15310)
                     /gene="mprF"
                     /locus_tag="IWM51_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9969721.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15451..16257
                     /gene="proC"
                     /locus_tag="IWM51_000274"
     CDS             15451..16257
                     /gene="proC"
                     /locus_tag="IWM51_000274"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP9969722.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16336..17892)
                     /gene="rny"
                     /locus_tag="IWM51_000275"
     CDS             complement(16336..17892)
                     /gene="rny"
                     /locus_tag="IWM51_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP9969723.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18303..19352)
                     /gene="recA"
                     /locus_tag="IWM51_000276"
     CDS             complement(18303..19352)
                     /gene="recA"
                     /locus_tag="IWM51_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP9969724.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19461..20702)
                     /locus_tag="IWM51_000277"
     CDS             complement(19461..20702)
                     /locus_tag="IWM51_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP9969725.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20942..21520)
                     /gene="pgsA"
                     /locus_tag="IWM51_000278"
     CDS             complement(20942..21520)
                     /gene="pgsA"
                     /locus_tag="IWM51_000278"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP9969726.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21729..22613)
                     /locus_tag="IWM51_000279"
     CDS             complement(21729..22613)
                     /locus_tag="IWM51_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9969727.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(23391..24677)
                     /locus_tag="IWM51_000280"
     CDS             complement(23391..24677)
                     /locus_tag="IWM51_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9969728.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24670..25938)
                     /locus_tag="IWM51_000281"
     CDS             complement(24670..25938)
                     /locus_tag="IWM51_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9969729.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26190..26651
                     /gene="mscL"
                     /locus_tag="IWM51_000282"
     CDS             26190..26651
                     /gene="mscL"
                     /locus_tag="IWM51_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP9969730.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26710..27156)
                     /locus_tag="IWM51_000283"
     CDS             complement(26710..27156)
                     /locus_tag="IWM51_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9969731.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDCLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(27249..27494)
                     /locus_tag="IWM51_000284"
     CDS             complement(27249..27494)
                     /locus_tag="IWM51_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969732.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(27609..28187)
                     /locus_tag="IWM51_000285"
     CDS             complement(27609..28187)
                     /locus_tag="IWM51_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9969733.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(28264..28794)
                     /locus_tag="IWM51_000286"
     CDS             complement(28264..28794)
                     /locus_tag="IWM51_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP9969734.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(28798..29658)
                     /gene="rlmB"
                     /locus_tag="IWM51_000287"
     CDS             complement(28798..29658)
                     /gene="rlmB"
                     /locus_tag="IWM51_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP9969735.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(29651..30046)
                     /locus_tag="IWM51_000288"
     CDS             complement(29651..30046)
                     /locus_tag="IWM51_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9969736.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(30043..31455)
                     /locus_tag="IWM51_000289"
     CDS             complement(30043..31455)
                     /locus_tag="IWM51_000289"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP9969737.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(31452..31988)
                     /gene="cysE"
                     /locus_tag="IWM51_000290"
     CDS             complement(31452..31988)
                     /gene="cysE"
                     /locus_tag="IWM51_000290"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP9969738.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(32310..33770)
                     /locus_tag="IWM51_000291"
     CDS             complement(32310..33770)
                     /locus_tag="IWM51_000291"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP9969739.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(33940..35097)
                     /locus_tag="IWM51_000292"
     CDS             complement(33940..35097)
                     /locus_tag="IWM51_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP9969740.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(35131..36501)
                     /gene="radA"
                     /locus_tag="IWM51_000293"
     CDS             complement(35131..36501)
                     /gene="radA"
                     /locus_tag="IWM51_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP9969741.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(36564..37091)
                     /locus_tag="IWM51_000294"
     CDS             complement(36564..37091)
                     /locus_tag="IWM51_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP9969742.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            37321..38127
                     /gene="proB"
                     /locus_tag="IWM51_000295"
     CDS             37321..38127
                     /gene="proB"
                     /locus_tag="IWM51_000295"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP9969743.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            38124..39371
                     /locus_tag="IWM51_000296"
     CDS             38124..39371
                     /locus_tag="IWM51_000296"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP9969744.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            39447..39791
                     /locus_tag="IWM51_000297"
     CDS             39447..39791
                     /locus_tag="IWM51_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969745.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(39908..40234)
                     /locus_tag="IWM51_000298"
     CDS             complement(39908..40234)
                     /locus_tag="IWM51_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969746.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(40607..41808)
                     /locus_tag="IWM51_000299"
                     /pseudo
     CDS             complement(40607..41808)
                     /locus_tag="IWM51_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            41925..42440
                     /locus_tag="IWM51_000300"
     CDS             41925..42440
                     /locus_tag="IWM51_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9969747.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(42639..43085)
                     /locus_tag="IWM51_000301"
     CDS             complement(42639..43085)
                     /locus_tag="IWM51_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9969748.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(43132..44610)
                     /gene="msr"
                     /locus_tag="IWM51_000302"
     CDS             complement(43132..44610)
                     /gene="msr"
                     /locus_tag="IWM51_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9969749.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(44964..45593)
                     /locus_tag="IWM51_000303"
     CDS             complement(44964..45593)
                     /locus_tag="IWM51_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9969750.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(45577..46041)
                     /locus_tag="IWM51_000304"
     CDS             complement(45577..46041)
                     /locus_tag="IWM51_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9969751.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(46528..47796)
                     /locus_tag="IWM51_000305"
     CDS             complement(46528..47796)
                     /locus_tag="IWM51_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP9969752.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(48027..48764)
                     /locus_tag="IWM51_000306"
     CDS             complement(48027..48764)
                     /locus_tag="IWM51_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9969753.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(48831..49400)
                     /locus_tag="IWM51_000307"
     CDS             complement(48831..49400)
                     /locus_tag="IWM51_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP9969754.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            49528..50400
                     /locus_tag="IWM51_000308"
     CDS             49528..50400
                     /locus_tag="IWM51_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9969755.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            50742..51614
                     /locus_tag="IWM51_000309"
     CDS             50742..51614
                     /locus_tag="IWM51_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9969756.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            51611..53569
                     /locus_tag="IWM51_000310"
     CDS             51611..53569
                     /locus_tag="IWM51_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9969757.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(53820..54671)
                     /locus_tag="IWM51_000311"
     CDS             complement(53820..54671)
                     /locus_tag="IWM51_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9969758.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            54794..55261
                     /locus_tag="IWM51_000312"
     CDS             54794..55261
                     /locus_tag="IWM51_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP9969759.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(55524..56006)
                     /locus_tag="IWM51_000313"
     CDS             complement(55524..56006)
                     /locus_tag="IWM51_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9969760.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(56106..56969)
                     /locus_tag="IWM51_000314"
     CDS             complement(56106..56969)
                     /locus_tag="IWM51_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9969761.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITKTQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            57567..58415
                     /locus_tag="IWM51_000315"
     CDS             57567..58415
                     /locus_tag="IWM51_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969762.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(58586..58927)
                     /locus_tag="IWM51_000316"
     CDS             complement(58586..58927)
                     /locus_tag="IWM51_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP9969763.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            59013..59720
                     /locus_tag="IWM51_000317"
     CDS             59013..59720
                     /locus_tag="IWM51_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP9969764.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            59707..60030
                     /locus_tag="IWM51_000318"
     CDS             59707..60030
                     /locus_tag="IWM51_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9969765.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(60119..61156)
                     /locus_tag="IWM51_000319"
     CDS             complement(60119..61156)
                     /locus_tag="IWM51_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9969766.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(61603..62586)
                     /locus_tag="IWM51_000320"
     CDS             complement(61603..62586)
                     /locus_tag="IWM51_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP9969767.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(63002..63772)
                     /locus_tag="IWM51_000321"
     CDS             complement(63002..63772)
                     /locus_tag="IWM51_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9969768.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(63765..64700)
                     /locus_tag="IWM51_000322"
     CDS             complement(63765..64700)
                     /locus_tag="IWM51_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9969769.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(65294..66943)
                     /locus_tag="IWM51_000323"
     CDS             complement(65294..66943)
                     /locus_tag="IWM51_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9969770.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(67138..68034)
                     /locus_tag="IWM51_000324"
     CDS             complement(67138..68034)
                     /locus_tag="IWM51_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9969771.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(68027..68707)
                     /locus_tag="IWM51_000325"
     CDS             complement(68027..68707)
                     /locus_tag="IWM51_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9969772.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(68708..69490)
                     /locus_tag="IWM51_000326"
     CDS             complement(68708..69490)
                     /locus_tag="IWM51_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9969773.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(69483..70400)
                     /locus_tag="IWM51_000327"
     CDS             complement(69483..70400)
                     /locus_tag="IWM51_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9969774.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(70403..70687)
                     /locus_tag="IWM51_000328"
     CDS             complement(70403..70687)
                     /locus_tag="IWM51_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9969775.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(71388..72008)
                     /locus_tag="IWM51_000329"
     CDS             complement(71388..72008)
                     /locus_tag="IWM51_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969776.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQIS"
     gene            72528..>72823
                     /locus_tag="IWM51_000330"
     CDS             72528..>72823
                     /locus_tag="IWM51_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9969777.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDT"
BASE COUNT        20956 a        15524 c        12835 g        23508 t
ORIGIN      
        1 ataccgagaa aatggttttt gaacatcact tattcaaaat aaaaaaagat tgtgacacga
       61 attttgtcac aacctttaaa tgtattccta gagcaaagaa gctaagcgaa gaaatccaat
      121 tctgattatt tattgtattt ctttgttcag catccttgct aaaagcccat attggacaaa
      181 gtattcacta cacccatgat ctcatctttt cgagaacgtg ctttttctgt cggatcttct
      241 acctcttctg tgtcaattcg aacaataaat ttccgattca tcgtcacgta agcaccgtcg
      301 ttgatcaaaa taaacagttc gttctcatta tagtctgcac aggcatcttt ccatacgtca
      361 aagccgtttt tttcagaagg gatatcccgt ttaaccacag tttttccact taattcgtgg
      421 aacgttacgt cataatattt cattatctct acctctttcg tcattcaata ttttgtactt
      481 gttctctgat tttttctaag atcgtcttag actggacgac ttgattttta atttcgattg
      541 catttgattt ggaaccgatc gtgttgattt cccgattcat ttcttggatc aggaaatcta
      601 attcccgacc tacaggacat tgtgcttcga taagttcatc tagtttttgt acatggatcg
      661 ccagacgatc cagttcctcg tgaatatctc cgcgttccaa caagatggct agctccgtca
      721 acaaccgttg ctcatcgact ttcgctccaa gaaattcctc cagctttttt tgatacctta
      781 tttgaaattc cttttcatag atttctacaa aactatcgag tttttttagt acttctttga
      841 attcttcagt attctttttc aacactaaag ctaaagcttg accttctttt tctcgactcg
      901 cctcaataga atcaagtgcg cttttcaatg tatctagagc taactgtgcc aattcttcca
      961 tgctctcttc cggtttttct tctaccgtta cgaagttttc gtccagcgcg aagcgttccg
     1021 ccatccgact aattgacact tgattttgac taccgtatcg ttgttgaatt ccgtctgtca
     1081 attgagtgat aagtgaatca atcaattccc agttgatgac gatctgtttt tcgtttttgc
     1141 caatataagc tagattcaca aatacttcga ttcgaccgcg gcttaactgc tgtttaataa
     1201 gctgacgaaa atcgttttca acaaagttca ggattttagg acttcgtatt tgaacatcta
     1261 gaaaacgctg gttgacactt tttatttcaa tatctaactg atacgctttt gtttcgcgga
     1321 tggctttgcc aaaaccagtc atacttttca ttcgacaggg tccttcctca ttacatattt
     1381 tctattgctt tttgcagttg ttcaaactct tcgttcgtca atgtcaggcc tttccccatt
     1441 ttttcatggt ctggcgccca atctcgaaga tcgaattttg gcgggcgacc attccagctg
     1501 actaggttca gttctttacg ccaccccttg ttgttttctg acaatatagc gatttcttct
     1561 acgatttcat atgaaaattc ttgtgccatg gttgttcctc cttgagataa tataaatata
     1621 ggatcagtaa tgtgtcaact ggttgcttaa gcaattcatg ctgcaaacga tccttgcgtt
     1681 ttccttgtag cagataatca aaataaacag ttttcgtgcg ctcattttgt attttttgct
     1741 ggtattttcg ataccttcgc cattcttcag tacttaattg accaatttgt gccaataaat
     1801 catccggcat actttttata tcttgttggc agatcatttg aatcatcatg tctacttgat
     1861 agatttcatt gggcagtaaa aaatgttttt ttgctaaaag ttctagcatc gccaatccgc
     1921 atgtctctgg tacgaccctg ttcttatata aattacgcag tttttgccaa aattttatgc
     1981 ttttgaaaaa agataattct aactgaattt cttttgttaa gtaagacaaa aatcctcgcc
     2041 agtatgttct tgcaggttta acagaatgaa atgtatacca gttcccatag taatagccta
     2101 atcgtttttt catttccaga tagcctaatt gtaaatttaa ttggttcctt tggctatcaa
     2161 aaagcagaaa gctgcctagt gtccacaatg tttgacattt ccaatggata aaaatgtctg
     2221 gcatacgaat ttttttcgct gggcctggcc cttgcacatc tacgacaaat gcctctgttg
     2281 ctcctttgtt aatcgcaata tcaataggta ttttgttgcg atacccaccg tctgcatatt
     2341 tattttttcc gatctttcga taagccatag cgggataaaa tgaagcagag gcgagtatcc
     2401 aatctggtat ttcttctgtt tttgtctgtt ggatatcagt cactacttcc gctagttctg
     2461 gcagtttagt agacaccgta tataaacgta ttttgggatt atgtttgagt aaggacaaat
     2521 ccaaattgtc tttgaccatc ttttccaatg gagcagaaga aaccccgcct tcaattagtg
     2581 ctgttttcgt catttggcgt ctttctcgat agaagcgttc acgaaggtct tcaactgcag
     2641 ccatttctgg taacgcaagc acttgtcttg tcgacagctt tttccataaa ttcacagctt
     2701 ttttcatatc tccttgtaaa atcaatgcgc cgttcaacgc acctaccgag gtacctgtga
     2761 taatctcaaa gttgatatca tgttccttca gcgcctgcca cacaccaatc tgataagcac
     2821 cgtgcgctcc tcctccacct aaaacaagtg ctgtatgata gaccagcttt ttatttaatt
     2881 gtgtggttcc ttgatagccg tttttatgaa aaatacgcaa ccattcttct gatagaggag
     2941 attgaaataa aaaacaacag ttttctttaa taaaaaaacg agcgcaagct tccatatacc
     3001 gaaaaaattt tttccattca aaagtttcac cttcaatcaa cagattatta aaaataacat
     3061 ctcctctact tttagcaata gtaaaataga cggtatattt tttcgatact gctttaaaag
     3121 cacattgctt ttttgcagaa cgccaaaaag cgtctctact ttcttgatga ctttgaaaaa
     3181 atggaagggg ttgttttgct tgctggatag gcgaatacag cgacattttc tgtgaaaagt
     3241 aaactttttt tatctccatc gcgctctcct ttttcttcaa gtataccagt acgtcataaa
     3301 aaggtcatga gtctcgtatt cgactttgta gattccataa gaaaacaatt cataaaacct
     3361 cctttaaata aaaaaataca aaaatcacaa aaattaattg aaaatgagag tgttttcaat
     3421 gaaaacgtga ttttgtaatg gaattttaaa ataaaagaaa caagcttttt taatgcaaat
     3481 gttgtaaaat aaaagagatt aagaggaaat aagaggagga acatgatgac tgagtcagtt
     3541 tatgtacata ttgataccac aagtaatgct gttttaacaa aagggctgac tgctactgac
     3601 tttgcaaaca gtatcgtgca ttttcctcaa aacttgctct tgcttgatcc atctgcttct
     3661 gctggagaat atgaatccca tacagggctg aaagtaatcc gtggtacaga aaatattcag
     3721 cgtttctttt cctcttctcg taacaggcgt ggattcgatg aattgaagtg gatcgacttt
     3781 actgatttaa cgatgctgaa agaattgaca cctttagaaa tttcagaatt attatatttt
     3841 ggtcatatga aaacacatct tcattcgccg tttttttata aactacagaa taattttgta
     3901 tacttcgatc ttaatgatca gctgaatcgg atttattatc gctatttaga tgaattttat
     3961 aggatttttg ctagtaaatt aagttcgatc ttgtcagata agatcaatgt aaaaaaatct
     4021 tttttcaaaa aagaaacacc tgttgaaaaa atcagtgtgg aattattgaa agaaatgcgt
     4081 aatttgatgc aggaagggat cattttttct tttgatcaag ttggtttgat cgatggtaaa
     4141 tatcgtatcc caatctatgt gattgaagat tatatttgga aattaaaagg cacccggtat
     4201 cgagaagagg aaatcatcgc tactttaatc tactctgtca ccgatcgaca ctggcaattg
     4261 gtagaagatg ccgatctttc ttatctgcca ttgaagtaga tctatttctt atcaaaaaat
     4321 aaaaaggaaa caagaaacta ggacatgatt ctgtctcaag tttcttgctt cttttttatt
     4381 tacagctcct gtcgtttatc tggcacaaaa atcgaaggag tcgtttgttg gatccctaat
     4441 aaaacgatga tcgttacaat agctgtactc aacccgctgg caccattcat tacgaaagaa
     4501 aagagccaag gattcatccc ccatagtgca tacgctcccc agaaaaccac tcctgcaata
     4561 aaatgccaaa aatatctgga aagtgcgcca atcaatgcac caaacacgat gctttttcta
     4621 atttgtttcg tattatcgtc tacatagcct ttttggacat ttgaagccat gagccctgct
     4681 gcgcctgcaa aagtataggc aatcgggtac tcaatcaaca cttgaacgac acttaaataa
     4741 taaacttgtc ccaacggaaa atgtaggatg ccccaaatga aacctgccag catccctgcc
     4801 tttgtccctc ttcttaacgc aaaaagagtc aatggaatct gccctaatga aatagaaaaa
     4861 ctgctgccga tctggatcgg aatcaacgac aacaccatcg ctaacgctgc aacaatcgtt
     4921 ccttctaccc aaactctatt ttttcccata aaaaaacttc ctcctttgaa taaaaaagtc
     4981 acaaaggagg aagcagcttt cgaaaatagt caaacagctg tttattcaca attcctacgc
     5041 tcgcataacc gatacaggta cgaagggttt agaaatagat tcaatctcag cccaattgga
     5101 ctcccctttg tgagtttatt tattatttat tttttgtttc aaacaatgga ctaactggtt
     5161 tgttttcatg gatacgtttg attgcatctc ccattagtcc gccaacactt acttcatcga
     5221 ttttgtcgat tttacgatct tctggaagat agatcgagtc tgtaacaact agacgctcga
     5281 tagctgaatc ttctattctt tgtaaagcag ggccggataa tactggatgt gtacatgaag
     5341 catacacact agttgcacca gcttctttca aagcattggc agccaatgtg atcgtacctg
     5401 ctgtatcgat catatcatcg atcagtacac aagttttgcc tttgacttgt ccaatgatat
     5461 tcattacttc tgcaacattt gcttttggac ggcgtttgtc tatgatcgca atcggagatt
     5521 tcaaaaattc cgctaattta cgtgcccgtg tgacaccacc atggtctggt gaaacaacga
     5581 caacatcatc gcctttgatt cctttttcta agaaataatt ggcaataagt ggcgcaccca
     5641 tcaaatgatc gactggaata tcaaagaaac cttggatttg aactgcatga aggtccaaag
     5701 tcagcattct tgttgcacca gctttttgaa tcatgtttgc tactaatttc gctgtgattg
     5761 gttcccgtgc acgtgcttta cggtcttgac gcgcatagcc ataataaggc atgactacgt
     5821 tgattgtttt tgcacttgct cgtttcagtg cgtcaatcat gatcagcaac tccatcaagt
     5881 tatcattgac tgggctgctc gttgattgaa taacatagac atgcgatcca cgaatacttt
     5941 cttcaatgtt tacttgaatt tctccatcac taaattgagt aacagatgat tttcctaact
     6001 cgacgccgac tgctgctgca attttctctg ctaatgggcg attggaattt aacgcaaaaa
     6061 tttttaatct tgggtcaaag taatgttttg acatggggac ctccactttc tttttcacaa
     6121 ctctagaatt ctacctaata aatagtagtc atatttttta aataaatcaa gtgattttgc
     6181 aaaagtctta gtttagataa gggagttttt ttgcatatcc ttctttattg acttgtcttg
     6241 ctctcgcaat tgccagcgca tattctggaa tattgtctgt aattgttgac ccggcagcaa
     6301 tcgaactgtt tttagctacc tcgacaggtc cgatgatatt cgtgctagaa ccaataaagc
     6361 tgtggtcgcc gacagtcgta tgatgtttgt tctttccatc atagttgacg aagacaacac
     6421 cacatccgac attgatttct tctcccagcg tagcatcacc aacataagtc aagtgaccga
     6481 ctttagttcc tttgccgatt tgagcatttt tcacttccac aaagtttccg atatggacgt
     6541 tttcgccaat ttcagctttt ggtcgtaaat gtgcataagg tcccacatct gcatgtttct
     6601 tgacatggct gctttcaatg actgagtttt caatgacgac gtgatcctca atcctactgt
     6661 ctacgatttt tgaatgtgac ccgatgacac aatcagaacc aatgactgta tttccttgga
     6721 tttgtacacc tgcttcgatc aatgtgtctg gaccgatttc tacgcctgca tcgatgtatg
     6781 tcgttgctga atcgacaaag cttacgccat tgaccatatg catctggtta attcgctttc
     6841 tcatgatctc atttgcttta gctaacgcga tccgatcatt gactcccatt gattcttcaa
     6901 aatcatccgt ttgataagca gcaactgtat ggccttcttc ttttaagatt tcgatgatat
     6961 ctgttagata gtattcacct tgagcattgt ttgttccaac tttgcttaac gcatcgaaca
     7021 aagcttcgtt gtcaaaacag taagttcctg tattgatttc ttgtacaaga gcttcttctg
     7081 gtgttgcatc tttttgttcc acgatttttt ctacgatccc aatatggtca cggatgattc
     7141 gtccatatcc tgttgggttt tctgcttgtg ccgtcaagat cgttgcactt gcattttttc
     7201 cttggtgata ttcaaaaaga ttgttcagcg tttcagtagt caataatggt gtatcgccac
     7261 tgataactaa cgttgttcct ttttttcctt ccaagaaaga agcagcttgc aaaacagcat
     7321 gtcctgttcc caattgttct gcttgtaacg catatttact gcgttctcct agttgtgctt
     7381 taacttgttc tgctccgtgt ccaacgatcg taatcacttc atctggtttc gtttcactga
     7441 cccgattaat gatatgttct accataggtt taccagaaac gggatgtaaa actttatata
     7501 attttgactt catgcgggta cctttccctg ccgccaaaat aattgcataa cgtgcgtcca
     7561 actgcgtcac tcctaaatat agtttgctga tttcagttta tcccagtata cccatttttt
     7621 caacaaaaca agtgattttc ttttatccca gcgttttctg gataaaaaaa tagatagacc
     7681 ataacgggcc tatctatttg gtaaccggtg ctctcctgcc aactcctcgg tcacaagtca
     7741 cactactcat aaaacatgaa aagctgtttt ttgaaatcgt ttcttgtgac ttagagttaa
     7801 acggtaggct tacaacctct tcattaaacg tttttcaatc agctgacctt cggtattagc
     7861 tcaataaacg gcaaaatatg aggagcaatt tttccgttta ttgagctaat actcaggaca
     7921 ggacgctgat ttcacaacct ctagataaac ggtgttcaaa agccagtttc tcggtattaa
     7981 gccaaattta tgaaaaaatg tggaaagcca ttttcacaaa atttttctta ctactcgaaa
     8041 ctaagcagct ttttcacaac ctcttattct gaaaaataat ttcctggcac aaccgtaata
     8101 tttttcgttt gagtatctac atctttgaca taaagcagtg acgtataatc atcgatggcg
     8161 cgatgaccgt tgaattttga ttcggcaaat acagtgatgc caacaagttc tgattcgaat
     8221 tcttcaatca agctcttcat tccgttgact gttccgccgc ctttcatgaa atcatccact
     8281 acaaggacac gtgaaccacg tttcaaactt ctcttagaca gttccatctt ttcaattctt
     8341 tcagaagatc cagacacata gttgacactt actgttgatc cttctgttat ttttgagtcc
     8401 cggcgtacaa taacgaatgg cgcattcaga taataggaca cagcttgggc aataggtacg
     8461 ccttttgtgg ctacagtcat tacagcatcg attttttcac caaggtattt agaagcaatg
     8521 attcttccaa cttgacgcag cagttcaggt tctcctaaca aatcagagag ataaacatat
     8581 cctcctggaa gcagacggtc ttgttctgat agacgttctg ctagagattc aatatatttt
     8641 ttcgcctctt catatgaaat ttcagggatg aacaatacac cacctgcagc acccgggatt
     8701 gtttccaagg ttccataccc tcgttcttta aatgttttct tgatgattcc caaatcttca
     8761 ctgatagaag atttagcaga ttcataacgt tcggcaaagc ttgttaagga aatcaattca
     8821 tgcgggtgat tcaataaata ctgtgtcatg tcaactagac gttcactgcg acgtactttc
     8881 acctattcca actcctttta agaaatttta tcgttttaca ttatataagt ttttcttcaa
     8941 aaattcgagc attttatgaa aaaataagaa aatagttcgg tatttattaa aataaaaaga
     9001 agcctataaa aaatttttcg tttttataag gcttcttttc tttataattt agttattttt
     9061 tcttctgaag gcaaccaata ttcgtttgat gatatttaca atcaggaaca aagcaataaa
     9121 gactaatgtg attgttgctc ctggaggtgt atctagataa aacgagctag ttagtcctga
     9181 cagcatccct agaaatccta cacaaacact gatagcaatc acgacattga agcttttgcc
     9241 taatcgcata ccgattgctg cgggaagaac catgattgcg gatatcaaaa gtgcaccggc
     9301 aatcgggatc atcacagcaa tagccacacc agtaatcaca ttgaacatca tcgacatcaa
     9361 atgaacgggt agcccatcca cgtgggcggt atcttcatca aaagtcagaa catacatcgg
     9421 ccgtttgaat aaaaagaaca atataccgat ggcaacgaat aagattccta aaaagatgac
     9481 ttgctcccat gtaatggtca cgatcgaacc aaacagatag gattggatac ttgttgctga
     9541 attccctccg ctcaaattca tcaatactaa cgctagcgcc aaaccaccag ccattaaaat
     9601 ggcaatggag atttctgaat aggttcggta gatcgttcgc agatactcca atatcacagc
     9661 tgctaataca acgactagca tggtcgtaag tgtaggatta aggttcaaaa agaatcctaa
     9721 cgctacccct gctaaagaga catgagaaag cgtatcagcc atcagcgatt gtcttctgat
     9781 cactaaaaac acacctagca tcggggcgat tccggcaatg aagatagcgg caagaaacgc
     9841 tcttctcatg aattcatatg aaagcaacgc cattgagaat cctcctttct caccagtcgg
     9901 atctgtctgt ccgcatactg tttgatatct tcatgatcat gggtgatcat tagaactgat
     9961 ttcccatgga catgcgcact atgtcggaga agacgataaa attcatttct ggattcttca
    10021 tccatacctg ttgttggttc atcaagaaca aataagtcag ggtcagtagc aaaaatacgc
    10081 gctaaactga ttcgctgctt ttgtccgcca gacaattcac ctattcgccg atttcgcata
    10141 tcccacatcc ctacagcttc taaagcacgc tgtacatgtt gatgatcttt ttcagagaga
    10201 cgcttgaacc agcggcctct tggatatctt cctgaacgga ctaattcgat caccgtacta
    10261 ggaaaacctg cattgaaaga tgcaacttgt tgaggaatat aaccgatact taattttttt
    10321 ccttcttgat ttttctcagc aatctttatc attccttttg aaggtttcag taatcctaac
    10381 gatgctttca ccaaggttga cttggctgct ccattttctc cagttaaaat gacaaattca
    10441 cccgcatcta catgataaga gacatcttct agtacaggtt catcatcata gtaaaaggtc
    10501 aaatcagata tttcgatata acgcacctta caacctccat tctaaaactt aattgatact
    10561 ttcttttaaa gctttcagat ttttttccat gacagaaata taattttcgc ctgctttgat
    10621 ttgttcatta gtcaagcttt ctaaaggatt caacacttct agcttcactc ctgtttcatt
    10681 cgacaatgtc tcagctacct tcgaagaggc attttcttca aaataaatca ctttgatgtt
    10741 gtttttcttc acaaattctt ttagctcagc taagcgactt ggtgtgggtt cctgatctgg
    10801 tgaaacgccc gaaatcgcta cttgttttaa gtcgtattga ttcgctaaat aagcaaaagc
    10861 agcatgctga gtgacaaatg tgcggtttgt tgcatctttt agctcatcgg agtatttttg
    10921 atccagtgct tcaagctttt tgatataagc atcggcattc ttcgtaaaag tttccttttt
    10981 atctggatat ttttcgccta attcttcagc aatcgttctg acttctttta tagctaatac
    11041 cggatcgagc caaacatgtg gatcgtattc atgtgaatga ccatgccctt cttctgattc
    11101 ttcatgttcg tctgcttctt gcgattctaa acggtcgatt cctttagctg cttcgatcat
    11161 caaggtctgt ttgctatcca ctgaatcctt taacgaatcc acaaaaaatt ccatgtctga
    11221 actattatac acgaaaacat ctgcatcgga aatttctgct ctttcttttg cacttggttc
    11281 gaaatcatgc ggctccgttc cagcagggat caaaagtttc acgtttcctt catcccctac
    11341 gatctctttt gtaaaatcat acatcggata aaaagtcgta acgatcgtca aatcctcttt
    11401 tttgtctgct tccttgttcg tatttccaca tgctgcaaac agtaaagcac ctatcattac
    11461 cgtagctgtc aaaagatatt ttttcattta tatgctcctt tcaatgaaca aatcggaatt
    11521 ttacgattta ttaacgtgaa ctaatttatc aaaataaaaa aacacagtca atagaaatcc
    11581 atcaatttct gatttaagca tgtatattct tgattaaatc gtaaaatgga tgagttgctg
    11641 ctcatatcta gccatttcac tacaataaaa aactgaagaa taccatttta ggattcctca
    11701 gtttttcatc acctatttta cttcttatat tttattgcag ggttcgtact aaataaactt
    11761 cctcacaaaa accttttaac ccattatata ctctctgcgc tcttgaataa tgttgacaca
    11821 aggcaaaaac ggttggtccg cttccactca tcaatgcagc atctgctccg tacttgatca
    11881 tacgctcttt gatttgatgg atgactggat gccgctttcc cgttatatct tctaaactgt
    11941 ttcccatttt tttgatcatc tgctgataat cttgctctcg gatggcttca gcaagtccgt
    12001 ggatgtcttg atgttggatg gcttgcgtat ctacttctct aaacactgta cgtgtagaga
    12061 cactcattct tggtttgacc aacacaaccc agcactgtgg catagaaggt aatacttgga
    12121 tttcttcccc tttgccaccg acaaatgccg tacttccgta gacacagtaa ggaacatctg
    12181 taccgacttc caaaccgatt tctgccagtt cttctaacga acagcccaga ttccataatt
    12241 ggttgatccc tcttagagca gctgcacagt ctgtacttcc tccgcctaat ccagctgcta
    12301 ctggaatatt cttcttaaca aagatcttga caccggtttt gatattgtat ttatttttta
    12361 gcaataaagc agcttgataa acattgttcc gttcatctac cggtaaaaat gctttatttg
    12421 tttctacaat gattttgtct tctgttattt cttcaaaaaa tagatgatct gccaagtcta
    12481 cgctcgacat caccatttcc aactcatgat acccatcttg ccgtctatat aatacatcca
    12541 gtcctaaatt tatcttggct ggcgcttttt caatcaattc catcctctca cccattcgaa
    12601 aagtctaagt cgcaagtatt ctatcatagg gaaaaataaa agtatagctt atgtctgcct
    12661 agtggttgat tttgtcatcg tttcaacagc tttcacagaa ttttctcttc ataatccaaa
    12721 caagttctag tgattcttat aattctgtga gcacttcttc tgccaaactc ggaacatttc
    12781 ctttttcttt tcctatcaga ctgagtagct gcatggtagc aaatccgaaa ttacttcctt
    12841 tcctataagc aaaataacgt ccttcccaat gatcgacata ttttaatttc ttttcataag
    12901 cagactggaa ggaatagaaa aaattaccat aattatatac gatatgcatg actttttctt
    12961 gaaagaaaga aaaatctgtt tctccaacat tagctaatgg agctaagcct agattggctt
    13021 ccgagtagcc cattttctcg tatacaataa acagattggc taacagatag tcagctaatt
    13081 cctcagattc tttaggtaag acccgcaaca aatcatatcc cgcctgcttc ttcgtcatcg
    13141 gctgagcggt gatgaagcca acgacttttt gatctttacg gacaataccg acaccgctag
    13201 ataatacata tgatttatca aatcgtccca cagagaagta tttttctcgt gcaccttgaa
    13261 gccatttttg tgaaacttca tttacagctt gatgtaattc ttccggcagt tgatcgtacc
    13321 aagtaaaatg gtatccttct ttcatcaaac gttgatattc aagttgctga ctcagccgag
    13381 acaagtcttc tgttgctaga tcaacaatcc ctgcttcacc tactttcgtg aagtgaaaac
    13441 ccaattcatg aagcatcact acgtagcttt ctgatatctt ataaaacgca agctgatagc
    13501 ctaaacaatc tgcttgtttc ataaactgat tcgttgctgc ccgcatcatt ttaggattgc
    13561 caactggatc tcctaaaaca aagcatttgt ttcctttgat ctgataacca aaaacgactt
    13621 gatcttggtc gtcttcttgg taataaaaat aactgtaacc tgacaagcga agctgatggc
    13681 ttgtagctgt gcctccgtat gtttccaata aacggtcaaa acggtctttt tcgtactcac
    13741 tgccaagctt cgtagtatct tcagctaaat attgataaag cgccactaaa gctagcaatg
    13801 agatgcccaa accaataagc cctgagaacc acacatcatc tgaaggaaac aaaataaaat
    13861 gatttcctgt tattcgaccg actaaaaagc gtccgctgtg atagcctgca attgcgtaaa
    13921 cgataaacaa aaagccgaac aagctagcat caaagattat agctccccaa gaataaacga
    13981 attttgttcg ataaaattct tttctggata accacacagc taacagaatc aagacatata
    14041 ccaatatcaa ctgccaagat aacgtacgtg tgattgtatt taaaatacca aaagatagta
    14101 aagcaatcgt aggcagaaat gcctttttga cttttactga aagtgctcta gcaaggccga
    14161 gtaataaaaa gccaaccaat agattcactg tctgatctaa aaaattgaat gagaacggca
    14221 ataatacttg aaatagccga ctgattgcag acagattagt gattgttgat agaagtacca
    14281 tcatgatccc cgcaaaatag acagcactta cgagcacgat atgcgcaacc ttttggaaaa
    14341 acagtcgcgg aatgttatct aaaaatcgat tgattttgac tcctgtatgt gtgatgaaca
    14401 atagaagtcc cgtcataaaa ggtagaacat aataaaacaa ccgataatat aatagccaaa
    14461 ctactgctac atcttgccga acaggcaatt gactcattcc taagatcatc aaaacgtcaa
    14521 aagtccccat tccacctggg accatcgtca gcattcctat caatgtagca atcatgaaca
    14581 taggatagat cgaaagcatc gaaatatcta ttctcatcaa cttgccgatg atcaaaaata
    14641 ccgcaagggc tcccgtccat tgtccaaagg atgccagtag cagaccaagc aagccttttg
    14701 gataaaactc gaagaacaat gtctttctct ttagataagc aaaaaagaaa atgactggtg
    14761 ctaaaaaact accgccgaaa agccagatcc aatagcttcg aaaaatactt tctgactgaa
    14821 taaaaaagac atcaataaat gtaagaaacg accatacaga caaaccagtc agcataaata
    14881 gtgcgacttt tgatacagtg gctaaaacca tttttctatt tgtctgcttt ccataaaaac
    14941 tagcccgcag acttgctcct accacaccac caaaaccggc gagattgttg atcgtgtttg
    15001 ttacccacgc agcaagaaga aactcttttc tttccatttt tggttttcct tgtttttcta
    15061 agactctgat cgtcacccaa tcatacaaca acatcggagt gacacccagc agtccagcca
    15121 gaatcatgag caggatcgtc atgcggcttt gttgtcccat cctttgccaa atatcttgcc
    15181 aactcatgcc attagcaata tttgcgactt gatttgccac aaagactaac acggagccga
    15241 aaaaaatcag ttttaaaatc agactatgtt cctttatcca ttgcagcatc aagtatcgtt
    15301 tatttttcac attttcccat tcctaacttg tatacttttg tgtcataccc caatgttatc
    15361 ttatttattt taatttttcc actttagacc tgttctttgc ttgcgatgat agaaaaagaa
    15421 agatatccta atgctaaagg agaggataac atgaaagtag gatttatcgg cacagggaat
    15481 atggcacaag ctattattga tggtttatta aaaaaaggaa cagtctcgaa agaagaggtg
    15541 ttcgtttcca gtggacattt tgaaaatgca caaaaatttg ctgctgaaac tggcgtccat
    15601 gcatgcaaaa gtaatcagga agttgcatca caatcagata tcgttctttt agccgtgaaa
    15661 ccaaaaatca ttgaaaaagt attggaagaa ctaagagaat atagccagaa agtcttattc
    15721 atttctattg cttcaggaaa atcattaaca gatttagaaa cagcgcttga agatcctcag
    15781 gcctccatcg tacgtgtgat gccgaatgtc aatgtcagcg taggagcagg cgtttctgcg
    15841 atttgcaaaa atgaagctgc cacggaggaa cagcttcagc aagctacgat gatttttgaa
    15901 gcaatcggca gcgtctatcc tttagcagaa tcagatttca gtacattcat tggccttgct
    15961 ggcagctcac ccgcattcgt ttacatgctg atcgatgcga tgggacggac tggtgtactg
    16021 aatggtttgt ccaaacagac agccactgag attgctgcaa aagcaattat gggttcttgt
    16081 caaaaattcc tttcaagtga taaaaatcca tgggaacttg tagaccaagt ttcttctcct
    16141 ggcggtacaa ctgttgcagg tgtagttgcc ttagaagaag caggattcat cccagcaatc
    16201 atcaaaggaa tcaatacgac gatcgagaaa gatcttgaga tgttgaataa aaaataatga
    16261 tagcaatgtt ttttcgtgcc ttacaaaaaa agaagagctg tggcttttgc cgcggctctt
    16321 cttttgtatt ttttattatt tagcataatc agttgcacga gtttcccgaa taacgattac
    16381 tttgatgtgt ccaggataat ccagttcttc ttcgattcgt ttacgaatat cacgtactaa
    16441 acgaacagcg tctaaatccg agatttcttc tggcttgacc attacccgaa cttcgcgtcc
    16501 agcttgaact gcgtaacttg attctacacc ggcgaagctg ttcgaaatat tttccagact
    16561 tcttaaacga cggatgtagt tttctaatga ttcacttcgc gcaccaggtc ttgctgctga
    16621 gagcgcatct gctgcagcta caaggacaga gataacagaa gtagcttcca catctccatg
    16681 atgagaagcg atcgcgttga ttactactgg gttttctttg tacttggcag ctaattccgc
    16741 accgatctca acgtgagatc cttcaatctc atggtcaagt gctttcccaa tatcatggag
    16801 caacccggca cgttttgcta attggatatc ttcgccaagc tctgctgcta agataccagc
    16861 taattttgca acctcgaccg aatgcttcaa gacgttttgt ccataactag ttctgaatcg
    16921 cagacgtccg atgatcttga ttagatctgg atgaagtgta tgagcaccga cttcaaacgc
    16981 tgcctcttcc ccgtattcgc gaatacgttc atccatttct ttacgagatt tctcgaccat
    17041 ttcttcgata cgcgctggat ggattcgtcc atcttgtatc agtttttcca aggtcatccg
    17101 tgcgatttct cttcggattg gatcgaatcc ggaaagaaca actgcttccg gtgtatcatc
    17161 gatgattaaa tcgataccag tcagcgtctc aagcgttcga atattacgac cttctcgtcc
    17221 aatgatacga cctttcattt catcattcgg aagggtaacg actgaaacag tcgtttctga
    17281 aacttgatct gctgcgcagc gttgaatcgc taatgataat aagtttttcg ctttgcgatc
    17341 tgcttcttct ttggcacgct gttcagattc ttttaccatt agagttaact catgattcag
    17401 ctcttcttca gtcgatttca tgatgacatc tcgtgcgtcc tcttttgata aaccagcaat
    17461 tttttctagt tctttttgtt gatcttcaat cattttttca acgtcttttt ctcgctcttc
    17521 aattaattgc tgttttgaac taagtgcttc ttctttactt tcaagtgagt tctcgcgttt
    17581 ttctaacgag tcatctttac gattcagcgt ttgttcgcgt tgaatcaaac gattctcttg
    17641 agattttaat tctaacttac tttctctcag ctcactttca acttcagaac ggtatttctg
    17701 attttcttcc ttagcttcta acaatgcttc tttttttaga gtttctgctt ctttttttgc
    17761 actatcgatg attccagcag ctgatgattg tgcaccagct atttctttct cgtgacgtga
    17821 ctttgcgacc ataaacccta aaccaagacc gacaattaaa ccgatgatag cgaggagaat
    17881 gttgaatacc ataaaatcca cctccatact atctttttca tttttatcat ctatacagct
    17941 tctatgataa attattctaa actcgtcaat atgcatcttc gcatatatgt gtacaaaaaa
    18001 catgcacaac tttattctaa tgtttgtacc ccgtagtgtc aactttaaat aaacaaagaa
    18061 acggctttct acttattcat taaacgatgg tttttaaaat aaatgacact agtttcatat
    18121 aggtaaacct ttaaattgag aaacttttct attaaattat tctttgtaga tgaacagata
    18181 tttttatttt atgtacccac tcgtataatt aaataactca cgatttcttt attcaataaa
    18241 aaaacggcag gtcaaggaga gtacctactc ttcagaccag ccgtttttgt ttatcataat
    18301 ttttattctt ctactaaagg caattcttct tgtccttttt catcttctgg gacagcgact
    18361 tcttcaccaa taccataagc tgctctaact agagcagaaa cttctgccat catttcagga
    18421 tgattagcca tatagatttt agcgttttcc cgtccttgac caatccgatc ttctttataa
    18481 gaataccaag caccgctttt atcaacaatg tctttttcta ctgccatgtc tagaagttct
    18541 ccttcttgtg agatccctaa accatacatg acatctactt cagcgatttt gaaaggaggt
    18601 gctactttgt tttttacgac tttgatcttt gtacggttac caacaatatc tgtaccttgt
    18661 ttcaattgtt ctgcacgacg tacttccaaa cggatcgttg cgtagaattt caatgcgcgt
    18721 cctccaggag tgatttctgg attcccaaac atcacgccta ctttttcacg gatttggttg
    18781 atgaaaatag cgattgtttt tgttttgttg atcgaaccag agagtttacg caatgcttga
    18841 gacatcaaac gtgcttgtaa cccgacatgt gagtcaccca tttcgccgtc gatttctgct
    18901 cgtggaacta aagcagcaac tgaatcgact actacgatat ctacagcccc acttgatact
    18961 aaagcatcag cgatctctag accttgttct cctgtgtcag gctgtgaaag aagtagttca
    19021 tcgatattca cacctaattt ttgtgcatat tgcggatcta acgcatgctc agcatcaatg
    19081 aaagcggccg ttccgccatt tttttgtact tctgcaatag cgtgtagtgc aactgttgtt
    19141 ttacctgaac tttcaggtcc atatacttcg atgatacgtc cacgggggta accgccgaca
    19201 cctaaagcga catctaacgc aagagagcca cttggaattg tagagatttg ttggtcgatt
    19261 ttttcgccca atttcataat agagccttta ccatagctct tttcaatttt tttcagtgca
    19321 gcatctaagg ctgctttacg atcatctgcc aatacgataa tcctccttat atttacaaat
    19381 cagagcaaca tttcattatc cttatcatat cttttttagt gaaaaaaagc aagaaaaaac
    19441 cgaacaaata ttcgcattta tcattttctt aaaagctgtt ttctcaaaag atcaaatcct
    19501 tgcataaccg cattatatct gatagcagca cgatctctgg caaagtgata tttttccgaa
    19561 accacgccgt ctttagatga aatagcgatc catactgttc ctgcagattg accttctaac
    19621 gtgtccggtc cagccactcc ggtgaaagct acactgtaat ctgttcttgc tatctcacgt
    19681 gcttgaatgg ccatctgctc cacacattct ttgctgactg ttccatattc tgctaatagc
    19741 tccggatcaa tctccagaaa accagctttt gtttggagcg agtaggtaac gaatccgcct
    19801 ggaaatactt cagatacacc agcaatgtcg cctaatgcgg cttggaaggc gccggctgtc
    19861 aaactttccg ctgctgttac agtctgcttg ttttctttca gtagttccac tacgactttt
    19921 gctagcgaat tatcatcgcc gtatccataa aaatattccc ctactcgttc ttggattttt
    19981 tcttctaatg ctaaaagagc ttgattccct gcctgaacat catttgtttt cactgtcagt
    20041 cgtaatgtca cttcatttgg tttggcatat ggtgcgatcg ttggattgat ctgtgtctca
    20101 atcagatctt ttagctcagt gaccagtcgc gattcaccaa tcccataaaa gcgcaaaact
    20161 ttagaaatta gtttttcttc agaaggaaaa cgttcctcta gtaaaggacg tacttgttcc
    20221 acaaacattg gtttcaactc attaggcgga ccgggaagaa ggatatacgc atgtgtatct
    20281 gtttgataaa aagtccctaa agcaagccct gtacgatttg gtagaggaac cccgccttcg
    20341 ataatctggg actgttgcag attgtttttg gtcatttttc gatgggttgt ttcaaaatat
    20401 gccaataatt ttttgtatcc ttccgtattt tgtatgagag acttaccgag gtgagccgca
    20461 gttgcttctt ttgtcaggtc atcttctgtc ggtcctaatc cgccacataa gagtatgagt
    20521 tcacttcttg tttcagctag agcaatcaac tcttctaaac gctgctgatt atctcctaca
    20581 actgtttgat aataaacttc gatacctaga tcagctagct gttctgacaa gaaagtggca
    20641 ttcgtattca cgacttgtcc tagtaaaagt tctgttccga cagctattat ttctgctttc
    20701 attacttccc gctcccttat atagtatacg ctgtattttt atattttttt atttaaaaag
    20761 agggcaagga gctgtgacaa aaatcttgtc acagctcctt gttccaaata aacagtgttc
    20821 tcctgccaaa acctcggtca taagtcacaa tatacataaa acatgaaaag ctgttttttg
    20881 aaattatccc ttgtgactca gagttagacg gcaggctcac aacctctcca ctaaacgata
    20941 cttacattga tcctttgaac acaccgctgt ttttgatgaa gtaatccaca cctgaataga
    21001 tagtaaagat cagacaagca taaagcatga tttgatctac tggaataccg atcaggctga
    21061 atgggatatt attgataaac agcaagataa tagcaaccat ttgtgtagcc gtcttgactt
    21121 ttcccggcca agctgcagcc atgacttctc catgttcgac aagtagtaaa cgcaagcctg
    21181 tgacggctaa ttcacgacag acaatgatcg ctactaccca accaggggcc tttccttggt
    21241 caaccaacat gataaaagca gtcataacta gcattttatc tgccaacggg tccgcaaact
    21301 tgccaaaatt agtgacaagg cctcgcgcac gggcaatttt tccatctaac cagtctgtga
    21361 tactagctaa agcaaaaata actgcaccta ctagctgtgt gacagccagt gtggtgtctc
    21421 ctacgttaaa ttgtccccaa tctaaaggta aacctacaac caaaatgaaa atcgggatca
    21481 tacaaattct aagtacagtt aatttatttg gtaaattcac actatcgact cctttcttcc
    21541 ctattctacg tgactatcag cgaagtttcc accgatatta tttacttttc caaatgaatt
    21601 tattcaaagg agtattttta ttgaaattga tctttttgaa ttaacgaaga aggaccgcga
    21661 cacagatttg tcacgatcct tcttttttat acctacaagt aaacagatgc gtaaactaat
    21721 ttatacgatc attctgcgta ttgaagagta aggttcacat tttttggact ctgattgttt
    21781 tcccctggat taactggtac ggattctcca ttcgctttga tcgttgcatt gcttgctgca
    21841 ccaatcgtga tggtcgcatt tgttgctgtt tccggtaaca cggtactctg tgtttcattt
    21901 gctgccagtg tcccttgata aacataggca ttgtctacta gcacacctaa ccatacacga
    21961 ttgacagcag taaattcaag ggttacaggc ttggtagcgt ctgtgatatt tactgcgatg
    22021 gcactgccag aatcttctcc tgtagctatt gccattttct tttcttcact tgattttgtt
    22081 gttgattcta ctgtagaaga ctctttggtc tgcgtgcttt gctctgagga agaggactcc
    22141 tcgactgatc cttctacttt aagcgaagaa gctgtgtctc cgattattgg atcagcttga
    22201 cgatcttgcc aagtcatata gcccacgaca acgacaatcg ccaaagcaac caatcctaat
    22261 aaaatgacag gaagacttgt ccaaaagcga cttggatgcg attcttcctg atgcattgct
    22321 tttctagaag tacttactgt ttcaggctgc ggacgttcag ggaccgatcc tgtcaatttg
    22381 cttttcccat caaaaacatc gactaaatgg tctccatctt ctcctacagc ttcagcgtat
    22441 tgccgaataa atgcccgcac gtaaaattta ccaggcaatt ggtcaaaatc atcattgtcg
    22501 attgcttcca aataacgttt ttgaattttt gtaatctgct gtaattcatc taaagatatt
    22561 tttttattca ggcgtgcttg ccgtaatttt tctccgatgg tttcaaaagc cactcgttca
    22621 tcttctccag tctgtttgat tctccgaagc atctgctgtt gcaacgaaag acgttatgtt
    22681 tgggtgctgt gacaaaagtc atgtcacagc tccttttttc cgaataaaca ttgttctcct
    22741 gccaactcct cggtcacaag tcacaatatc caataaatat aagaagctgt tttttgaact
    22801 tgttccttgt gactcggagt taaacggcag gctcacgacc tctttcttaa acggtgttca
    22861 atcagctgtt cctcggtatt agctcaaaaa ccggcaaaat ataaggagat atttttccgt
    22921 atgtttctct aatactcagg tcaaaacgct gatttcacaa cctcttcctt aaacggtgtt
    22981 caaaagcttg catctgcggt aataagtcca accaagttaa aaacattaag aagctgtttt
    23041 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct tggtacacgg
    23101 tgttccagaa gtagcttctt cggcaataag ccgaaatatc acaaaaattt gagaagcaat
    23161 tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tctttgatta
    23221 cttcgtttga ttttatttta atataagttg attaaaagag agcatgattt gttttatttt
    23281 ctcactaatt tttccaacac aactaagaat accataaaag ctgattttgg aggaggttcc
    23341 tactacaagt ctaaaacgaa atttaagact tttcctcata aaatactggt ttattttggt
    23401 ctcatgtaaa actcgctgaa ggcctcttca cttataaatg cttccccagc tgccagtacg
    23461 tctttcattt ggatcgaatc gatgatttca ggaagatcaa ataaagttcg atcgccaaat
    23521 aaactttgcg taaattgatt ggcaatatat tcgatcgaat ttaatgattg gaaatattgt
    23581 ccaagcattt tcttcttcag caaatcgaga tttgtttctg acacttccgg atcatcagcg
    23641 aatccaagaa tgatttcttt gactttttct gctgctttct ctggttcgtc cgtatctccg
    23701 ctgaagtccg caaagtgaaa ttcacgatcc aagctgaatt caaaaccaaa gctgtcatcg
    23761 ataattcctt gattgtacat cgctagatag tttcgagaag tattgcctaa aatcatttgg
    23821 aacaacagat tgattgctgt tttatagcgt aacagttctg ttccttcttg cggcaatgtg
    23881 tctaatcctt taataccaag tacaaattta tctcgtgtaa tggctgcttc caatgcactt
    23941 tgtttgatga tttcttttgt gttttcaggg aaataacgta caatttcttg ctttggaggg
    24001 aaattttttg cttcttggtt ttcccgaata agtttcatca atttctcagg ctccatctta
    24061 ccaactacaa ataaaaccat attgcttggc tgataaaacg tccgatagca cgtatacaag
    24121 tcttgagctg tgattttgtc gatactttca accgtgcccg caatatcgat atgcaatggg
    24181 tgtgtaggat acaagttatt tagtatgcca aaaaacatcc gccagttagg gtcgtcttca
    24241 tacatttgga tttcctgacc gatgattcct ttttccttgt ttacagtttc ttctgtgaag
    24301 taaggtgctt gtacaaaatc gatcagagtc gtcagattct tttctacctg atccgtcgta
    24361 gaaaacagat agctggtttt cgtaaaactt gtgaaagcat tggcggaagc tccttgtttc
    24421 ccaaataatt gaaagacatc accatcttct ttttcaaaca acttatgttc taaaaaatgg
    24481 gcaattccat ctggaacttt caccttttct ttttcaccat agggaataaa ttcgttatcg
    24541 atcgaaccat aattcgtact gaacaaacca tatgtcttgt gatagtcatt tttaggcaac
    24601 aaatacacgg tcaaaccgtt tggcagtact tcatgataaa gtgtttcatt gatttgctca
    24661 tattctgttt tattcatttg catttcctcc tgctaaaaag aagattgcct gcaattcgat
    24721 ttgctcagct acttgttgta tctctttgat cgttacgctc tccatacgtt tcaaccattc
    24781 ttcatcagat aattttgttt ccggcagcca gctgtccaaa aaagaagctt cgattgccgc
    24841 ttgcggagag tctagagaaa gcagatattg gtttctcagc attgcttttg tctgagacag
    24901 ttccaactct gttatttcac catttcgcaa actttctaat tgctcgtgaa tcagatgaag
    24961 tacttgattt cggttctttt catcgatacc ggtttggaca ctcatgaatc ctcggaacgt
    25021 atccacgcta ctagaggcat aataagctag actttctttc tctctgacat tcatgaaaag
    25081 tttagagtga ggaaaaccgc caaataaacc gttaaagacc attagcgcaa aacgttctgg
    25141 ttcatcgtaa tacacatttg tctgataagc taggttcagc tttgcttgaa cgattggctc
    25201 acgtacttgt cgttccttca cgatattcac ttgaggttgg gtataaaaca gatctggata
    25261 aactgtttcc cgcgactcaa atggtaattg cccaactgct tcagctgctt tgtccggatc
    25321 aacatcgccg acaacaaaaa tgtcgatttg atcatttgcc atcatttctt gatagtagga
    25381 cgctagcgaa ctagcagtga tcttgtccaa atcttcgact acgcccaacc ctgggatctt
    25441 ttgttccggg ctgttttcaa aataaagttc ttgcaaggca taggaagcaa aagtttgttt
    25501 atcttccttc aaactttcca aatatagacg cagattgttc ttttccaagt caaacgtcaa
    25561 ctgatcaaac tgatgatttt taatatttgg ataaaataaa acctctttta aaaaatctac
    25621 cgcttgagaa aaaaggtttg gatcattgac atattcacca ttcacaaaac tgatccctgc
    25681 gtttagccaa tgtaaatttc ctttttttct aactgataat ccaaagcttg cgccataaag
    25741 ctctgcaagt tgcgcactta actttgtctg atccggataa ttcaaactat ttgtttccaa
    25801 catgctgctg agaagtgaac gtttcgtgat gacttcttgt tgcagacgcg cagtaaaacg
    25861 gataaaaatc cggatcgttt tgtattgttt tgttggtaag acatgaagat tcacgccttc
    25921 tgccaattga tacaccatat ttatatgctc cttttcacag ttgattgccc gcttactctt
    25981 ttaatgggtc aacctgcctt ttttacttat tcctctgctt gaaatttata agttcaggca
    26041 attcgccgtt ttttctaaat gatgttacac tttatagtat agcatagatg ttttttatca
    26101 aaagaaacag acggctaaaa gcgtatattg tttttttatt ttttattttt cgatatacta
    26161 aagacaaata aaagaaggga gggtcattca tgatcaaaga attcaaagag ttcatcatgc
    26221 gtggagatgt cttggatctt gctgtcggcg ttgttattgg tagtgcgttt acaagcatcg
    26281 taaaccaagt ggtgcaaggc ttgatcacac cactaatagg ttggtttgtc gcaatgatca
    26341 caggtacttc tgatttagaa ggagcacttt caattcttga ctggtcgccg acaaaaggcg
    26401 tcacatttgc gtttggtaac gttattagtg ctatcattac atttctgatc actggttttg
    26461 tcttattctt agttgtcaaa gcggcaaacc gtgcgaagaa tttacgctcc aaagaagagc
    26521 aagtggaagt ggaagaaact cctactgcag aagattatct tagtgacata cgcgatcttc
    26581 ttgccaaaca agtggcagaa gacaatacac atccagtaga gcaaacaaat ctttccgata
    26641 cttctaaata ataaagaatc taaaaaaaag aaagccgatt ttcagtgtcg gctttctttt
    26701 tttagattct taacttgcta cttgttcctt tttcatgtcg atatgtggaa tatcatcttc
    26761 caaatacaca tctgagattg ccttgaatcc aaacgactca taaaaagctt gtaggtattc
    26821 ttgtgcgccg atcacgattg gtttttctgg aaaatttttc tggatcatgt taatcgtttc
    26881 ttgaacaatc tttcttccta aacaatcttt acggttgtcc tgatgaacaa ggacacgtcc
    26941 aaaatggata ctctccgctt cttcatatac tcgcgtgtag gcaaggatct tcccatcagt
    27001 atcttgaaaa aacgtatgcc aagcttgtcc atcaatgtca tcaacttctt gataaggaca
    27061 ctgttgttcc acaacaaaaa ctgcgattcg ttctttgacg atcgcgtaaa actctgctgt
    27121 tgacaattct tcaaatttct ttacctgata ttccactaag caaacgcccc ctgctttttt
    27181 attcttagca taccgattcg cacacaaaaa agcaaacgaa ccttacgaat ctcaggctcg
    27241 tttgcttttt aaactgcttc agttaaaaat tcgatctcaa ctgtccgagt aagtacatct
    27301 gaatagctgt aagatactcg ttcaaaagaa ttttcttcag gatccaaatc aacaacgaat
    27361 actgaaggat aggtttctgt taaaacgccg cggcgttctg tttgacgctt tcttcctgtc
    27421 tgagcaacca acatgattgg gctgccgata cgaccttcta aatctttctt gattgaagct
    27481 aaagttgttg gcatacagtt cacctctact atcaactata ccacatattt ttaaaatttt
    27541 caagtttacc ataaaaaaaa aagaaatgca ataaaaataa ataagaagct cattaagatt
    27601 tttctaaatt attcctcatt ataaaatttc aagtgttttt ttattttgag ttttactctc
    27661 ccgtaagcgc tcattacttg cttgtgatcc attgctaaac attcagccat gtctacaaaa
    27721 tcaacgcctt gcatataata atgaaaaatt ttagcttcta tttttgaaaa ctggatttcg
    27781 caattttcga ttgcttcttt taataaaaga tgctgaatcg ctaaaggttc ttgcaatgtg
    27841 atctctgcta aaaagtctgg tccttctgtc tctatttttt ggtcgattga gacttcatct
    27901 ttttgatcac gacgcttcag cgcgttctgc ttcctcaaca gagaatagat ttgattgctt
    27961 ataatgcttt tataataact tccaaagcta acacctttgc ttttttcgta agttctcaat
    28021 gcctgatata aagcgatcct tgcttcttgt tcccaatcct cagaaacata atttcttaaa
    28081 taataggtgt ttttcaaacg tagtaaaagt ggaaaatatt tttgatacag ggcagcaaat
    28141 gcttcttcgt caccattacg tgcagcgcgc aataactcct cagacataaa ataattcctc
    28201 cataaattcg tttatggagg aatcttatca tcaatgattc atcgaactga ttagaacgat
    28261 atgttattta ttttttctca ttaaatcgtc caatttttgg gacaattttt ttaactgctc
    28321 ttcattccat ggagaattac gtcggaaatc aataaaatgc atatctttat tttcttgggc
    28381 tattgttttt tccgtttttt ttatcgtctt atataattca taagcagaag ttcgtaatgc
    28441 tccttgggaa aagatcatcc actgttctgc taaatcgctt gtcgccactg tcacttgtgt
    28501 caaacggtca ttcagctgac cagcgattct ctcgatataa ctgtcagccg tttcgtcttc
    28561 tttcgtaaaa acaacatcca actggtattt cttataattt tgttggatgc ccggaactag
    28621 ttgggcatcg aagacaacga tgacttccat tccttcatat ttcgcataat ttgataaacg
    28681 atgtaaaaga gcttctcttg catccgccat tttgtcctgc tttttaagct ggacaagttc
    28741 cggccacgaa ccaatcatgt tgtagccgtc tacaatcaac agctgttttt tcatggtcta
    28801 ctccccctga cgttgggaaa aagcctgata cataagcaaa ccagcagcaa cactggcatt
    28861 taagctctgg acgtgcccgg tcatcgggat cgtcagcaat tcatccattt cttttttcaa
    28921 tcctggactc attcctttgc cttcattacc aatgactaaa gcaattgatc ctttggcatt
    28981 ccatttttgg aagtttgtgc cggtcatatc tgtgccaaat acccaaaatc ctgtttcttt
    29041 gagctgttgc acagcttgta ccaaattcgt cacgcgtgca acagggacat gttcgactgc
    29101 cccagtggaa gtctttgtta cgataggtgt gattccaact gctcggtgtt ttggtatgat
    29161 cacaccatcg acgccacttg cgtctgctgt ccgtaaaatc gacccaaaat tgtgtggatc
    29221 ttcgatgcta tccaaaatca aaaagaaggg gtcttctttt ttctcttttg ttcgttcaat
    29281 cagttggtca agggataaat attcataagg tgtaatcgca agaacgactc cttgatgcac
    29341 gccgtgatcg ctcaaagttt ctaatttttg tttaggaacc catttgactg gcacagcttg
    29401 ttcacgtgcg atttctttca gttcttctac tttttctcct cgtgaatctt cttgaagaaa
    29461 gagtttattc cctcgccctt gttgtagcgc ttctactgcg gcatggtgcc caaagacgaa
    29521 attatcttct aatgcttctt cattttgatc agaaactgac tgatttcgtc cagtttgctt
    29581 tttatttgaa tatctatttt gttttttgtc ttcaaatcca cgtttcgatt gatgtttgtt
    29641 tgttctttct ttacccattt ttttcaccaa ctttctgaat acaccagcgg atcaattctt
    29701 ccatccgctc ggtttgtttt gtcaaatgca gatagcccat gagcgcttcg aaccctgttg
    29761 cgacccgata cgtcgtaaca tcagcatttt ttgcgctcgt atggctttta gcatttcttc
    29821 ctctgcgata ataaagttct tcttcttctg tcaaaagatg ttcttccagc atttgctgaa
    29881 tcaaaaaggc ttgtgcattc gcagaaacat aattcgttgc cgcacgatgc agcttattgg
    29941 gttttgtttc tccttgatca atcaggtggt cacgaacata gatctcgtaa atcgcatctc
    30001 caacataagc caaggctaaa ccgttcaatt gtgtgtaatc tctcatgctt ctcttctcca
    30061 tcgggttcct tgcggtgtat cttctaataa gatccctttt tctttcaaca aatcacgaat
    30121 ttcgtcgctt cggacaaaat cacgattgct tcttgcttga tttcgttctt cgatcaattg
    30181 ttcaatctct tcatctaata actcttctgc tgtaaataaa ataccgaaga tcgaaagcca
    30241 ttgcgagaat aaagcttctc cttgtgccaa gacagcggca gaaactttcg gctggtctag
    30301 ataagtgttc agccacttag caagctcata gaccactgtg atcccatttg ctgcattaaa
    30361 atcatcgtcc atttcttcga tgaatcgctg ttctaaattt tctaacaaag cgattttatc
    30421 cgcatcatct gccaaattct ctgaggcagt ttcttggcgg aatttcaaat tgtcaaacgt
    30481 attcttcagt cgttgtaaat tggtggctgc ttctttcatc gtcgtttcac tgtagcggat
    30541 cggacgacgg tattgtgtgg tcgccatgaa gaaacgcaag acttgtggat caacgttttt
    30601 gatcatttca tgtacagtga tgaaattccc tagagattta ctcatttttt catcgtcttc
    30661 gccaatcgtt acatagccat tgtgcatcca gtaattagcg aattttttcc ctgttttcgc
    30721 ttcactttgt gcgatttcat tttcatgatg aggaaattct aaatcttgcc cgccaccatg
    30781 gatatcgatc gtttctccta aatgttttgt tgccataacg gaacattcaa tatgccaacc
    30841 gggacgtcct ttgccccatg gtgaatccca agaaatctcg ccaggttttg cttgtttcca
    30901 taaagcgaaa tctaatgggt cttctttttg ctgctgttct tcccctgtac gctgacttgc
    30961 tccgacttcc agttcatcga tggattggtc acttaatttc ccataatttt caaatttacg
    31021 ggtacggtaa tacacatcac ctgaagattc ataagcatag cctttttcaa ccaacacttg
    31081 aataaatgct aaaatatctg gcatatgatc catgactctt ggatgcagtg tagcaggctc
    31141 gacattcaac gctttcgtat cttcttcaaa tgctcggatg aaacgatcag caacttctgg
    31201 tgctgtaata cctaattcgt ttgctgtacg gatgatttta tcgtctacat ccgtgaaatt
    31261 cgatacgaaa tcgactttat agcctcgata ttcaaaatag cgtcgaattg tatcgaaagc
    31321 aatggtgcta cgagcatttc cgatatggat ataattatac acagtcggtc cgcagacata
    31381 cataccgaca tgtccctttt gtatagggat gaattcttct ttttttctgg tcaatgtatt
    31441 gtagattttt atcattctga tctcctcact ttttctcctt tgattcgaac gacttttgct
    31501 ggtactccta ccgctgtagc gtcgtctgga acatctttca acacaacggc acctgcaccg
    31561 attttggcgt tttttccgat cgttaccggt cctaatatct gcgcattcgc agagagcatg
    31621 gctccttgtt taacagttgg gtgtctttta cccgtttctt ttcctgtgcc gcctaatgtt
    31681 acgccatgga ataaaatcac atcgtcctcg atttctgcgg tttcgccaat gacgattccc
    31741 attccatggt cgatgaacac gccagttcca atcgttgctc ctggatggat ctcaacacca
    31801 gttaggaaac gccagaattg agcattgatc ttcgccaata aaaagagccg gtgctgatat
    31861 aaaaaatgag agaaacgatg ccaaaacaag gcatgaaaac cagggtatgt caaaacgatc
    31921 tcagctgtcg aacgtgcagc cggatcgttt ctctttgctg catcaatcgc tcgggtaaac
    31981 caactcatga ctttccactc ctttcgtaag aattttatgt aggaaatcat cttcttataa
    32041 agaaaagaag cgccttttaa ataagtttcc ttatcaaaag acgctttccg cgtggttcca
    32101 cttttcttca taatcaatga ttatctctga cagctaacgc ctgttatacg ttcttggcta
    32161 ctttgtttcc tataaaacag tttttcctgt tttctgtgtt tcgccaagac cactcccaga
    32221 ggcatttcgg caaccattca ttgatacctt gcaccacccg gtatctctct tgtaatgatc
    32281 agctgcttac ttttttctgt tcatcgtgtt tagttcagta cttgatttaa atgtgctaat
    32341 gatttttcac gaccaagcaa ttcgatcgtt tcaggcaatt ctggaccatg catttgtcct
    32401 gatacagcta cacggattgg catgaaaaga ttttttcctt ttacgccagt ttctttttga
    32461 acagctttga tggcagcttt gacagttgga gcatctacta cttccatttg ttctagctgt
    32521 gctttgaatg cgtggagaac agtaggaact gtttcaccag caagtacttc ttttgctgct
    32581 tcattcaata caggatgttc attgaaaaat agcgtagaca gatcaacgat ttctgcagca
    32641 tagctcattt gaggttgata caggctaacg atcttcttca cccattcgat tgtcttttca
    32701 tctgggtttt ctgctacacg tccatcagcg attagataag gcagacacat gtcagtaaat
    32761 gcatctagat ccattgcttt gatgtaatgg ttgtttaccc attgcagttt tttcgcatcg
    32821 aaagctgcgg gtgatttact taggcgtttt ggatcgaaca ttttgatcaa ttcttggtgt
    32881 tcgaagattt cgtcttcgcc cacaggtgac cagcccaata atgcaataaa gttgaacatt
    32941 gcatctggta gatagcctaa ttcgcgatat tgttcgataa attgcaagat tgattcgtca
    33001 cgtttgctta gttttttacc tgtttcactg ttgatgatca atgtcatatg tccgaaggtt
    33061 ggtggtgtcc agccaaatgc ttcatagatc atcagttgtt ttggtgtatt ggcaatatgg
    33121 tcatctccac gtaaaacgtg agtgattttc atcaaatgat catcgaccgc aacagcaaaa
    33181 ttataagttg gcataccatc gcgtttttgt atgacaaagt cgccaccgat attatcggat
    33241 tcgaacgaaa tagcgccttt caccatatct gtaaatgcgt attctgtatt gcgaggcaca
    33301 cggaaacgta caacagattc tagaccttgt gcttcttttt ctgcttgttc ttcaggtgtc
    33361 aagttcgcac atgttcctgc gtaatgaggc atttctcctc ttgcgcgttg tgcttcccgt
    33421 tctgcttcta attcttcttc tgtgcaatag catttgtatg ctcgattgct agcaagcagc
    33481 tgatcaatca gcggttgata gatttcttta cgttcagatt gacggtaagg gccgtattct
    33541 ccaggatttt caggagattc atcccaattc attcccaacc aagcaaggtt ttccaactgg
    33601 cttttttcgc catcttcgat attacgtttt tggtcagtat cctcaatacg aataataaag
    33661 tctccatcat tgtggcgggc aaacaaataa ttgaataatg cagttcttgc atttccaata
    33721 tgcaggtgcc cagttggact tggtgcatag cgtacgcgta ctttcgtcat tttcttcctc
    33781 ttctttcaaa ctagactttg actaacagca aaaattccgt cagttacggc ttctcaaata
    33841 tcatttattt aaagccactc agcacaaatt ttaccttttc taaggtaaat agtaaagcca
    33901 actggttgaa tttccttacc agttggctga atttcttttt tattttgatt gtttagtctt
    33961 tttctctgtc tcttttttat tgtcgattcc tcgtcctgca tgtgcaggtt tagcaaagat
    34021 catgcgtcca gcagctgttt gaagggcact tgttacaacg acttcgatac gttcattcat
    34081 gtaatgctgt ccatcttcta cgacaaccat cgtgccgtca tcaaggtagg ctactccttg
    34141 ttggcgttct gtcccggctt ttactactag aacattcatc gtttcaccag ggatgacgac
    34201 tggttttacg gcatttgcca acgcattgat attaagaaca ggtacatttt ggaattcaga
    34261 gactttattc aagttatagt cattggttac aatgacacca tctaaaagct tagccaattt
    34321 gatcaatttg ctgtctactt cactgatatc ttcaaagtct ccatcgtaca tttcgacaga
    34381 tataccctct tctttttgca aggcatttag gatatctaat ccgcgacgac cacgcacacg
    34441 tttcaaacta tcccctgagt ccgcgatgta ttgtagttcg taaagaacaa aattagggat
    34501 catcaaggtt ccttctaaga atccagtctt tgtaatatca taaatccgac cgtcaatgat
    34561 cacgcttgta tccaagattt tatatttatg gaaattttct cctgattttc gttctaacag
    34621 ttgactctca acttgactag ctgtttcttg ctcgattttt ttgcttcgtg aaccaaaaac
    34681 tttcttccat tcttcgattc gtgtcgttcc cattctcaat cccagatagc ctaagatgat
    34741 catcagtaaa atcggcaata cactattgac gaatggaatt tccatattgt aaagaggggt
    34801 cgagatcaca acacctaaag tcaatccgat gatcgcaccg atcgcaccga agagaagata
    34861 agtcaagctg acctcactta gtttttgttc tacttgtttg ataatagctg agatatgttt
    34921 ggccaacagc aaagataata aaaagaaaat aagtgcgcct aacaggctgt tggtaaatgt
    34981 attattcagc catccgtttg actgttgacc gattgcctgc caagctgagg gtaaaaatga
    35041 aatacccaag cttgcgccga caatgatcat cagcaaagta atgacacgtt tttgcatcgt
    35101 ttttcctccg tttcgacaat catttattag ttagcggaac acacgcttca acgtttcagc
    35161 caaagttgct actccaacta tctcgattcc ttttggcgct tcccagttac ctaagttgtt
    35221 tttaggtagg tatacttttg tgaagccgag tttttggact tctttgactc gttgttcaat
    35281 atgactgaca cgtctgattt ctccagtcag tccaatttca ccgatgaaac attccgttgg
    35341 ctgtgttcct ttttctttat agcttgaagc aatactgaca gcgatagcta aatcaatcgc
    35401 tggttcattt aattttaccc cacctgcagc ttttaagtaa gcatcttgat tctgaagaag
    35461 caacccagct cttttttcca gcactgccat gatcagagat acccgattga aatcaagacc
    35521 tgttgttgtt cgtttggcat ttccgaacat agtaggtgtg actaatgctt ggatctctac
    35581 caaaattgga cgtgtccctt ccattgccac aacgatagca gagccagttg caccgtctag
    35641 gcgttcttct aagaaaatct gtgaaggatt agcgacttct tccaatccat gctcatgcat
    35701 ttcaaaaatc ccaatctcat tcgttgagcc aaatcggttt tttactgctc gcaaaatccg
    35761 aaaactatga tgttgttcac cttcaaaata taacaccgta tccaccatat gttctaacat
    35821 tcttgggccg gcgatcgagc cttctttggt aacatgaccg acgataaaaa tggctatccc
    35881 atttgtttta gcgattttga gtagttctgc agtggtttct cttacttggc tgacacttcc
    35941 tgccacactt gtgatgtctg gctgcgtcat cgtttggatg gaatcaatga taacataatc
    36001 aggggagata tgttcgatgg cacgactgat ttcgttcata tctgtttcag cgtatagata
    36061 aaattctgtg ttgatacttc cgaggcgttc cgcgcgtaat tttatctgct ctgcgctttc
    36121 ttcgccggat acatataaga cttttccgcc tatggctgct aattgctgag aaacttgtaa
    36181 cagcaaagtg gatttcccga tacctggatc gccaccaatc aagaccatag accctggcac
    36241 cacacctccg cctaacacac ggttcagttc ttctaatttt gttttgatcc gaggttcttt
    36301 tttagggacg acgtctgcga tttttgttgg ttttgctttt tctcctgtca aacttgtccg
    36361 agttctgcgg tccgttgtat cttgttcgat ttcttcgacc atcgtattcc attttccgca
    36421 atttgggcag cgtcccaaga acttaggcga cacatacccg caattttggc aaacaaattg
    36481 tacttttgat ttttttgcca ttgtaccgcc tccttttatc ctaatcatga gacattaaat
    36541 caagcttaag tatctttaac gttttattta ctggaagaac caaatccgcc ttgacgttga
    36601 gcagtcgttt gatcttgatc tgcttttaaa aacggcaaaa agatcccttg gccgatacgc
    36661 tcgccttttt tgatcactac atccttgaaa ccaaagttgg tgaattggaa catgatatgg
    36721 ccttcatttg cctcattatt gtaataatca ctgtcaatca cacctactcc gtttgtcaac
    36781 actaaaaacc gcttcaacgg attgcttgaa cggttagcaa gctgcaaaaa ttcatcttct
    36841 cccatgtaag ctttgattcc agtaggcacg agggttggtt tcaattgttt ttcattttct
    36901 gatacatctt ttttcaatcc atctgcgact gtttccaacc cctttttcca gcctgctttc
    36961 cagatacttg gaatcacgac ctcttctgct gcttcaaaat catagccagc agcatgatgt
    37021 gtagcgcgct gcggtagatt gatattttga tctttatatt tagaaataac ttcaaatcct
    37081 ctttttctca ccggttttcc tccctaaata aactactcct attctaacat atccgaggct
    37141 gtaaaagact ttctttacaa attcttcaag aaattctgct atcttaaagt aaaccattaa
    37201 ttcgttcttt ttccaaacga ctgcaagttg tacaaaaaaa ctatttttta catgacttta
    37261 ctcgtcaaaa caagtagaat agaacgaaat taatgagtgg atatttgatt gaggtgtcac
    37321 atgcgtaaag aaattaaaaa tgcaaaacga attattgtca aagtaggaac aagtacattg
    37381 atttatccca atggaaatat caatcttgcg gcaatcgatc agttagcttt tgtcctttct
    37441 gatcttagaa atcggggaaa ggaagtgatc ttagtctcat caggagctgt aggcgttggt
    37501 atgcataaat tacatttgtc agaacgtccg actttgattc ctgaacagca agctgcggcg
    37561 gctgttggtc aagccgaact gatgaatatc tataatcaaa gattcagtgt atataatcaa
    37621 cagacggcac agatcctcgt gacaagagac gtggtagatt atcctgaaag tcgtaaaaat
    37681 gtgaggaata cattggaaca gcttttgatt atggacacga tccctatcgt gaacgaaaac
    37741 gatactgtag cagtggatga attagatcat ctgacaaaat ttggcgataa tgatcagctg
    37801 tcagccatca tggcaaaact tgtcgcggca gaccttttga tcgtcctttc tgatatcgac
    37861 gggttttatt cagataatcc gactacaaat cccgaggctc agatgtatcg ctatatcgat
    37921 aaaatcgatg aaaagattct ggcacaagca ggtggtgctg gtagcacatt tggaacaggc
    37981 ggtatgcaga gtaaattaaa agccgcttct aggatatttg aagcaaacag cgcaatgatt
    38041 ttagcaaatg gaaaaaatcc ggcaattatt tttgatattc ttgcaggaaa agaagtaggt
    38101 actttattta aggaggactt gaaatgactg acttgatgca attaggacaa caggcaaaag
    38161 atagcgcgca acaactagca cttatgccta caaaccgcaa aaatgagcta cttgttcaaa
    38221 tggcacaaac catcaaaaag aatcaacaag cgatcattga agcaaatcat aaagacttag
    38281 aaaaagcagt cgaaaacaat atcagtgaaa cgatgcaaga ccgcttgcag ctgacacctg
    38341 agcgaatcaa tgccatggct acagagatcg aaaaaattgc ttcgttagac gatcctattg
    38401 gaaaagtaga tgagatgtgg acgaacaccg acggattgag aatcggcaaa aagcgcgtgc
    38461 cactaggcgt gaccggcatc atttacgaat ctcgtccaaa tgtcacgaca gatgcagcta
    38521 gtctagcatt caaatcaggg aatgccgtta tccttcgcgg tggcaaagaa gcattcttct
    38581 cgaatcaatt attagtccaa ttattgcagc aagtactggt cagtgctggc gaatctcctt
    38641 atgccatcca gtttgtcgat gatacttctc atgaaactgc aggacagttg atgcgattga
    38701 cagaatactt agatgtgctg attcctcgag gcggagcaaa attaatccaa cgagtcaaag
    38761 aacaggcaac ggtacctatc attgaaacag gtacaggaaa taatcacgta tatatcgata
    38821 aagaagcaca gttatctatg gcagtgaaca tcatcgtcaa tgcaaaagct tcccgtccat
    38881 ctgtctgtaa cgcggcagaa acattgctga tccattctga gatcgcacct tttttcttgc
    38941 cagctattga aaaagaactg gtagaacacg gggtctcttt acgtgcggat gctcgtgcgt
    39001 tagaatatct agagacggct gctttagcag aagacacaga ttgggatact gaatatctag
    39061 attatatcct tgctgtcaaa gtggtcgatt ctttggatga agcaattgca catatcaatc
    39121 ggcataatac taaacattca gaaacgatcg tgacagatag ttatgcggct agtcaacgtt
    39181 tcttaaatga agtggacgcg gcggctgtct atgtcaatgc ctccactcgt tttacagacg
    39241 gatcggtatt tggttttggt gcagagattg gtatctctac tcaaaagttg catgcccgtg
    39301 gtccaatggg gttaaatgaa ttaacttcca caaaatatat catttatggc gatggacaaa
    39361 tccgcgaata atgtttcgtg tgaaacatta ttcccgcctt ttgccttttt tctgtaaagg
    39421 ttctatccaa ctagaaaggt gtcaatatgt ttgaaatttt aatgattttt tttatttacc
    39481 ttatttctct gaatatcgct gcttttctgg gagtcggtat tttatccctt ttctttcaat
    39541 tcaaaaaacg aagcataggg agtcaacggg aaaaatggtc gcagtatttt gacaaaatcg
    39601 ggcaaaaagg gctggttaca cgcttgcaca ttagttacat ggttgcatta tgcttattag
    39661 cgacaatcaa ttactattct tttttcgatc attcaatcgc ttatacgatt acgctactga
    39721 ttgctgggat ttttcactta tcttataaat atcagctgaa taaaaaccac ttgaatcgga
    39781 ctttccgcta aaaaatatcc gtacgataag tagagtgcct tgaagatcgg caaaaaactg
    39841 ccggactttt tagaggctgt gacaaagatt ttgtcacagc ctctttttcc atataaacgg
    39901 cgttcgccta ccagctgcct ggtaaatctg tttcctcttc gttaagaagc gaaaatccgt
    39961 caaatgtttc gccatcataa taacgaagca gctcatccat ggacaagttc atcgtttttt
    40021 ctttttcttc ttccgctgat tcagccagta gctgttcccg atacacctga tacataccag
    40081 ctattttttg ctgcatcgct tttctagttt caccttccgc attgattatt ggcaattctt
    40141 tgatatagcc acgataaatc aaccgatcat ctgaaaattc aatgggtaat tcaacgagtg
    40201 tcaacccgac ttcctgaaaa tattcttcca gcatctatta tccccccatt ctttttctta
    40261 aatgaatttt agcttaccta ctgaaaattt tccagtttca ttattttgag aaaaaaaggg
    40321 aatgtgacaa aaatcgtgtc acatcccctt tttccgaata aatggtgttc ttctgccaac
    40381 tcctcaatca taagtcacaa tattcaacaa acatgagaaa ctgttttttg aaattgttcc
    40441 ttgtgattca gagttaaacg acagtctcac aacctcttgg taaacggcgt tcaaaagctt
    40501 gcatctgcgg taataagccc aaccaaacga aaaatatgag aagctatttt tgtttggctg
    40561 ttcttatgac ttgatgcagg acagcttttt cacaacctct gatttcttat tcttcttcat
    40621 ttgtagcaaa ataattacgg tgataaatga tacaacatac actgataaga acaatggcac
    40681 ctgcaaaata aaacgcatag ctttcaccaa tatgttcacc aatcacacct gcaataaatg
    40741 gtgctaaagc tccaccgaaa aagcggacaa aactatagct ggacgaagca acgtttcgtt
    40801 gcagacctgg tatttccata gcaatcgtcg tcaacagtgt attgataatt ccttggaaaa
    40861 atcctgctaa cacaatgctg atcggtacaa ggactggcat agaagttcca gctcctaata
    40921 acagcaaaca aataatgaat aagacaaatg ctgctaaaat tgtccgataa gtcgtaaact
    40981 tccgctcaag agccggtgca acgaaaatcg aagcaatagc cagaagcaca ccccagccaa
    41041 aaaatacaaa accgacttct aattcagaat agccaatcaa taaaaatggc gcatacgcaa
    41101 gtaaagtaaa gaatccgaaa ttatataaca atgcgattag tccaactgaa cgaagtttat
    41161 gattgcttaa cgctttgaca ccttcccaaa ggcctacttt tctctttggt ttctcagttt
    41221 caggtaaaag taccgctagg gctataaaac cgcaaaacat caaaaatgct accccaaaaa
    41281 atggataacg ccatgaacca ctgcccaaga ccccaccgac tagagggcca acagccattc
    41341 cgcagccaag tgctgcttca tacatcatga ttgctttttc tgtgttacca actaaaatac
    41401 tgacgatcgt tgctaaagca gtagagacaa ataaagcatt cccgacaccc cagccggcac
    41461 gtaacccaat cagcattccg atattcccag ataatccgcc aagaccagcg aatacaacga
    41521 tgataaacaa tcctgtcatc aatgtttttt caaaccgatc cgactagata agaaacctgt
    41581 aaatagcatc accacaccag tgactaacat gtaactagta aataacaggg tcgtttcagc
    41641 tggcgtagcg tttaactgtg cagcaatcgt ttttaagatt gggtctacta gacccactcc
    41701 cataaaagcg atcatacagg tagctgctac aactagcgct gaacttttcc cctttgtttc
    41761 ttcatttgtt tgttcttgta tatctatttg attctccaat atttccatcc ctttctctct
    41821 attagcatca tacatgattt attttacatg taaatatgca catgtaaaac gtggaaactt
    41881 tactgtgata taataaagaa aataagacga gaggtaggat ttttatgaat actttagcct
    41941 atatattatt atgcgtgctt ttatccaaac cacgttctgg gtatgaattg aagcagctga
    42001 tcacaatttt ttgggaagcc catcacagtc agatctatac aacgcttgcc aagttagacc
    42061 agcagggtta tgtgaccgtt ttggacaaag acgagcactc tcaaaaaaag atctaccact
    42121 tgactgatga aggacgtgct cttgtagagg aatggatcaa agaggaaact cccgctccta
    42181 cgcaaaaaga tgaatttcta gcaaaaatct atgctttttc gacgctcgat aaaacaacag
    42241 ctactggact gctattcact agagaacaac agctgaacaa aatcatgcaa aaaaatcaaa
    42301 aaaaattgaa tgacctctcg ctagacaaga aacaagaatt tggacgttat gttgtgatcc
    42361 agcgcagaat tatgctttgt cagcaggaac tccaatggtg tcagcaagta agagaacaaa
    42421 tgcagcagtt ttttgagtaa gggataaaca gaacctttct agaaatatct attgaagagc
    42481 aaattatctt caataaaaga gcaatacagt tggaaaagaa aaagcgatcc gaacgacagg
    42541 ccaaaaccta tcgttcggat cactattttt ctagaactgc attcaagaga ctatgacaaa
    42601 aagcatgcca aggccttttt tcaaataaca gtgttccatc attcattact ttgaattgct
    42661 tttagcacac ttcttgaaag cgggccaacg atgatcaact gcaacggtaa agcggcaatc
    42721 acattcattt tccaagcatt catataagca gagaataatt gatcaggtat tccttgttcc
    42781 atgatcacac cgaataaaga catacatgtc accattccta aaaccatcaa gcatgaaata
    42841 gttaagatca tttggacctt gctttctgga tttactggta gtttaaaggc aatttttttc
    42901 gccagcacac cgatgatgaa gacatccaaa atgaaagcca ccacgaatcc aggcactaga
    42961 ccaatcaaca aacttgataa atgaagatct tgatgcagcc ataaattata cgcgctcatc
    43021 cccaagacca tcaaaaaaca catcattgtt gtaaaaatta ttccttcttt tttatttgta
    43081 ggcaatctgt accactcctt taaatttgtc acgaaaatca tcgtagcaca tttaaaaatt
    43141 tctcgtaagt acttttttta ttttctgccc ttttatttca taaacttcat cagccgtacg
    43201 ctcgacaaag taagaatcat gagaagtaaa caaaatcgtt cccggatata tttgcattag
    43261 cttctctaaa gcttcgatcg ttgccatatc aataaaatta gtcggttcat ctaacagcaa
    43321 cacattactt ggcttagtaa aaagcaaagc aatcgtcaga cgggtcgctt ctcccccact
    43381 taatgtacat aacggtttcg tcaaagtttc ctgagcaaac cctaagtgat tcagcaaact
    43441 acggacaagc gattctgaaa actctgtttg tctcatgaca aaacgaatga tggattcttc
    43501 agatgtcatt tgataaccct tttgctgata tacttgaaag cttactttcg gagagagaag
    43561 gattccttct ccttgtttgc ggatatgttc taataaggtt gtcttacctg aaccattttc
    43621 accgacaatc gcgatttttt tacctaaaga aaactgaaaa tcaccattta ccagtaatgt
    43681 tctcccactt ctttccaatt ggacattttg tcccatgatg ggatagcggc tgtggagttc
    43741 aagagatttt ggtactggaa aacggatttg tttcatctgc tgcggcttgg ttgtttctcc
    43801 tattttttgg agtcttctct ctaatgtttt cgcattcttt tggatggctt tttgtacact
    43861 gtctttttgt ttagaggaag acaaccgatc cggtctgatt tgttgctttt tctttttact
    43921 ggacactttt tccaactttt ccgcttgttt tttcttttct tggatcgatt tttccaatcg
    43981 ttttttctct ttcatgatcg actctgcttc tcgtagctga gtctttttct ccaattcctt
    44041 ttgagtgaga taggcactat aattccccga aaactctcgg atttctccgt cttttacttc
    44101 ccagattttc gatgctagct catctagaaa atagcgatca tgactaacaa aaaccagcgt
    44161 accataataa taacgaagct cttcaatcag tcgatcaata ctttttctat ctagatgggt
    44221 tgttggctcg tctagaagca tcccttgttc ataagaagaa agaagacgta tcaactcgat
    44281 ctttcgttct tcccctccgc tccatttccg ctctgaagtt tcttccagtc ttaactggaa
    44341 atgactggct aattccaaat ttaggtcctt tgcttcagcg agataggtta attgaggcag
    44401 gtagttgaag gtgatctctg tttggatttt cccatgatcg ggaaaaagtt caccggctat
    44461 tagtttgagc aaagtcgatt taccgacacc attttctcct atgatcccta tacgatcttg
    44521 ctgatagaca cgaagagaat caatagatag ttctagctgg ttgccaaagc tgacttgtag
    44581 atttgttata tttactgcta aattttccat atgttttaca ctcctatttc atgtaaaaca
    44641 acaattatcg atactaaaaa agagcagaaa aatctgtttg caaaaaaact gcatcacatc
    44701 ttttcctact ctgaacgaaa taactatgac taaatcacca aatctgtcat agctttcacc
    44761 tattctactg tcgttcgaaa aaatggacat actccatcca tgaaattgtt gtttatacat
    44821 gtcttttgaa tttatttagt tgttattcaa aagttcgaaa cgtaatttca tcgatgcagt
    44881 catttttccg acctcctttt ttattaggtt accacacacg attcaaaaaa agcaatagct
    44941 actgaatagt aactgaaaac cttttattcg aacgtagtga tcttttctgg tgctaaacga
    45001 acagactcgt aaccttcttc taccgctttt cctattgata aaataataac tggaacataa
    45061 cggtctttat ccaaaccaaa tgcctctgcc aactgatctg cttcgaatcc accaattgga
    45121 tttgtttcat agccatgatc acgcgcaact aacatgaatt gcatagccgc taagctagaa
    45181 tcgactttga ctacatcgtt catttcttct cttgaaaaac tcttgtaata aggaatgatc
    45241 gcagctaatt gttgatctct gacttctttt ggcatcttcc ctgattcgta agcttggtta
    45301 tagatttctt cgccatattc gtaacattgc atatcgccaa aaatcatgac cattgcagaa
    45361 gaagtatcat tttgtctcgt attgaaacgg atcaaaggac gcaagatttc ttttgcttcc
    45421 tcgctttcag cgaccacaaa gcgccatggt tgcatattga cagaagaagg tgcagtcgtc
    45481 gcttctttga tcatttccag catttcttcg tgtgggattt tgtatgtttc atcatatacg
    45541 cgaacggatc ttctaccagt cataattgtt gaaaaatcat ttttttgttc cattattatt
    45601 aacctctttc gtttctgttg ttatattgca aagttgggta ttcaatttat ctaaaagtgt
    45661 tagaaattgt ttcatttcta catcgctgaa agccggggat agcacggaac ctgtatcttt
    45721 atggtccatt tcacaatgct cgattttctt ctgggctttt tctgttagcg ctacatacac
    45781 ttcccggttg ttctgaggat ttctctgacg cgaaacatag ccatttgctt ccaatacctt
    45841 cagatgacga gtaactgctc cttgatcaat ctgcaacttt tcttgcaaac tgctttgtag
    45901 caatggattt tcttctttca gtctcatcat gatctcgtat ctagtaagac ttataccgat
    45961 attttcttca aagatctgga cgattttttg atcggctaat ttgatttgat ataaaaggtg
    46021 actaatctct tctaaagaca taatttcctc ctttctaata attgactcgt caacgattga
    46081 cttgtcaata atagaataaa aaaagaaaaa agtcaaagaa tcagctgtgt aataaaaaaa
    46141 acgctctcaa tagaaaagta tagagaaaag aaattttttg tagaatgaac ggaaaaaatg
    46201 ataaagaaag cacaacgcta aaaccaagat tattgattct tatcaaacta tagaagatag
    46261 ctcttctgca gtagaaaatt ttacaccgcc aaaaatttcc gaaatcgcct tttgcaagaa
    46321 tcccgtcgaa tattagaaat tggatcagta tgcttaaaca gtaataaaaa agttatgaca
    46381 agatgttgtc acaacctttt ttgagtaaac ggtgttcaaa aacttgcatc tgcggtaata
    46441 agttcaacca aacaaaaaac atgaaaatct gtttttgttt ggctgttctt actacttgat
    46501 gcagggcagc tttttcacta cctcttttta ataaccataa aatcctattc cttcatatct
    46561 ccagcccatc ggtgttagcc aatctctttc cttagcactc aaggtatagt gatgggtacc
    46621 agttttttca tttggattaa atagtcgata aattggcttg gaaccaccag aataaaaact
    46681 tagaccttca taacgccaac cgatttttgt taaagagttg atctcagatt gtgccattgt
    46741 gtaatgatgg tctccactgt aaggattgta taaacggtag acaggttgtc cgctgtttgg
    46801 tgcttgccaa gcaatccctt catattccca gcctacagtt ggcagataat ttttttctcc
    46861 ggcattcaat gtatataaat gttctcctgt atgacggtta tacagacgat acatcggaga
    46921 agttgaagtt ggtgctgtat catactgtgt cagattatag gtttcgatta cattgttcaa
    46981 tgtagaacca taattaggcg cagtggcata acgacctgtt agccaagccg tagcatcttt
    47041 gtaagagttc gtattgcttt tccatgcacc ggaatagtag taaacgcctg gctgaaaaga
    47101 agtcgtcttc aataccgtta cgttatcttg caatgattct ttgtacgaag gatattttct
    47161 aaaagcggca ttcaccgtaa tccattgccc attcacatat tccgaagtag gcatcgtaac
    47221 agattggcca ttatagcttc ctttaatccc aaatagattg taattagggg cagaagccaa
    47281 agtactgttc ccccagccac tttcaacgat ggcttgagca atcatgactg atgcatataa
    47341 gtcattggca cttgccaaac tttgtgcagt tgaggcaata ctatcaatga acgcttgttg
    47401 agaattagca gatgagcgag cataaaaagc atagccagta tagttttcga attcagacaa
    47461 atctgcttga tgggtatgtg tcaatgtttg cccatctgtc aattggttcg tgatttcttc
    47521 tgcattctct ggattgttct gagcttctgc tgatgtactg tcttcggttt gtgtttgttc
    47581 tgttgtttca gttgttggtt cttcggttga aattgtattt tctgagacag tagctgactg
    47641 caaagtactc tctgtatcgt tttcaattcc tgtttctgaa gaactcgatg tctgagttgc
    47701 gacagtttct gtcgattctt cagctagcac tgttgtgttg gcaactgcta gatttcctaa
    47761 aagaataacg ctaaataata aattctttgc tttcatcttt tttctccttt gaattatgaa
    47821 acttttccta aataacagtg taactaaaaa agtaaaagca agtcaatgtc catatttaaa
    47881 taacatttgt ttctttttgg taaatttgct ttttctacat aaaaagacgt aaatgaagaa
    47941 tattcttctc cattcacgcc ttaaattgat ggtcgtttga ataactattt aacaagtaat
    48001 tctctttgga tattatccgt tatggattat acttcctgtg tcgtcggacg gataatcatg
    48061 ttactgacag acatccggtc tggcgtttct atcgcaaatg ctacagcaga agctatatct
    48121 tcggaggtca atccccattc tttttgcgct tcatgaagtt caagagcagc ctttttgtcg
    48181 ctgatggttt gatataattc agtttgaaca gctcctggag agatgatcgt agatttgatg
    48241 ttattttgcc gttgctcttg acgtaatcct tccatgattg cacggacagc aaattttgtt
    48301 ccgcaataaa cggcggaatc aggataaaca acatgtcccg ccacagaatc agttgaaatg
    48361 atatgcccag atttttgttt ttccatgatt ggaagtacgg cggaaattcc atttaacaca
    48421 cccatgatgt tgatatcaag catattttgc cattcatcac ggtgaccttc aactaatgga
    48481 gcggttggca tgattcctgc attattgtac aatacatcga ttcgaccgaa cttttccatc
    48541 gctaaatcga taactgcttg gacttgtgca aaatctctta catctgctgt ttgaatatat
    48601 agctcagctt ctggtaaaga ttcttttatt gcttttaggc gatcttctcg gcgtgcagct
    48661 ataactagtt tagccccttt ttctgctaat agcttcgttg ttgcttcacc gattccgctt
    48721 gaagcgccca tgatgacaat cactttttct gataaatcat tcattctatt ctcctcctgt
    48781 tttgaaaaat ctcttgctta tactcactat ctcttatttc atcttactgt tcaaataatc
    48841 cgttcgacag gtttgacgat tttggcaggg attcctgcaa cgatggtatt gtctgggaca
    48901 tctttcgtaa cagttgcatt cgctgcaaca atggcattct cgccaatcgt tacgcctggc
    48961 agaatagttg ctcctgctcc gatccaagca tttttcttga tgtggattgg ctttactgac
    49021 aagcctcttc gttcttttgg atcggttaga tggttcaccg tgatgaggtt gacacgagga
    49081 ccaatcaata ctgaatcttc taaacagatg ccacctaagt caacgaacat cacttgacga
    49141 ttgataaaga catttttccc tatactaata tgtttgccga aatctgtata aaaaggcagc
    49201 agaacagtca ccgtatcatc tactttgtca ctaatgatat tttctagccg tttgcggata
    49261 tctgctggtt catggtaccc agtattcagc gctgctaaag gctcttcgtt ttcagcttgg
    49321 atctgatgga tcacttcaaa gagcggatcc gttactcgaa tctctttatt gacgatttgt
    49381 ttttcgatga ggcttgtcat tttgctccct ccttttatcc ttactttata acggacggct
    49441 tatcatgtct aatacttata aaacatactt aactattcct tttaactata cttagtggta
    49501 tgctttttta aaaacaggag ggaaaaaatg gatattcgta tactgacgta ttttttagca
    49561 gtagctagag aaaaaaatat cagccgagca gcaaaatctc tgcatatcac gcaacctact
    49621 ctctcaaaac aactgaaaga tttagaagaa gaacttggtg tgacactttt cactcggggc
    49681 tcccgagaga tacagttgac ggattctggt cgatatcttt attcaaaagg aaaagaaatc
    49741 ttgtcgttgg tagaaaaaac ggcgaacaat ttaacaaatg acgcaatcat ctctggagaa
    49801 atctacattg gcggaggcga aactaaagca atctctttca tttctgatag ccttcattcg
    49861 ttgatcaagg cgcatcctga tatccagttt catttatata gcgggaatgc agatgatatc
    49921 atagacaaac tggatagcgg attacttgat tttggactcg taatcgaacc gacaaacaaa
    49981 caaaaatacc agtcatttcg actgccagcc caagatcgtt ggggactttt gatacatcga
    50041 aatcatcctt tagcaaagca aaccgtcatc catccgcagg aactgaaaac tgttcctttg
    50101 atcatttccc ggcaaacgtc agtggacagt caactagctg aatggctagg tttcagcata
    50161 gatcagttat cgatcgtcgg aacatataac ttattgtaca atgcttcttt gatggtcgat
    50221 aacgggactt ttggtgcatt agctctagat gggatcatca atacttatgg gacggaaaaa
    50281 atattcatcc cactcgatcc gccattacaa gcttcactga atctcatttg gaaaaaagac
    50341 cagcctcttt ctcctgctgc tcgtaaattt ttagatgtcc taacggagaa atacagttaa
    50401 gtagagtttt tttccttcgc tttcagcttt ttaacaacga ataagataag caaaagatcc
    50461 aacgattaaa taacataagt ttcttaattt atttcttcct tttatttctg ataattccag
    50521 taaattacta taattataaa aaaagaattg acaaaacata actgtctata taaaattgat
    50581 tacgttaaca ataattcgtt tcatgtgact agatcatact aggcatgaaa aatctctgag
    50641 gaacatggtt tccctaggag gtttttcgtg tctatttttg tttggccgca atcaactggt
    50701 ctagcaaacg tgaaaaccaa atcttcaaaa agggtgcgcg catgaaaatc aaaaagatct
    50761 ttaatcaaaa tgcagtattg gtagatgacg gtggacagga aaaagtagct attggaaaag
    50821 ggattggatt tgacagaaaa agaaatgatt tgatttttga tcgtgatgtc gaacggatat
    50881 ttgtcatgga accagaaggt caaatcaagt tgcaaaattt acttaatcag attgatgaac
    50941 gctttttatt tgcagcagaa acgatcattg atcatgcgga aactgttttg atggaaaagt
    51001 tgaatgaaca tgtattgatc gctttgaccg accacattgc tttttctgcg gagaacatta
    51061 ataacggtat cgtcatccgc aacaaacttt taagagaaat cgaagtgctt tacagtgatg
    51121 aattcagtat tgctcaatgg gcagtagagt atctgacaaa agagcttggg attccatata
    51181 cttatgacga agcaggatat atcgcaatcc atattcacag cggacgaagc ggtcggaaca
    51241 ataaccatcg cagtatccgt gaagtgacga ttgtatctga catcatccat ttagtggaat
    51301 cagaattgga aacagatatc cattcagaac cattttcact gaattattca agactggtga
    51361 atcatttacg actgcttcta caacgaaccc atgcgcaaca atatgctgtt ttagacacgg
    51421 aaatcgtaga aatggtcaaa cgaaaatacc cagaaagcta taagatttct aaaaaaatcc
    51481 gtgtgttgct gaccaaagaa taccagctcg cgatcacgaa agaagaactg ggttacttgg
    51541 ccatccatat cgagcgttta agaagcgcaa tcgtacaaac aaatgttaac aatcatgagg
    51601 aggaaaaaaa ttgaaagcat atctacaacg aatgggacgt tcccttcaac ttccagttgc
    51661 ggtcttacct gctgctgctt tactagtagg gatcggaaac tggtgggcat cttatagtaa
    51721 cgacattgtc gctcattttt tacaagctgg cggtaacgca gttttaggtc aattaccttt
    51781 gctgtttgcc gttgggttag cactagggat gtcagtggac aaatcaggtg ctgctgcctt
    51841 ggcaggtttg gtcgcatttg aagttccagt aaatgtttta aaaactgatt cagtagctac
    51901 tttattgaat atcaaaccag aatcagttga tccatctttt gctcagatca gtaacgtatt
    51961 tattggtatt attgccggat tgattgcggc agctctttat aatcgtttcc acgaagcaaa
    52021 attaccgatg gctttatctt tctttagcgg caagcgtcta gtgccaatct tggctgcctt
    52081 tgtcatgttg ctgatcagtg cagtgctact gctcgtatgg cctccggttt actctgcttt
    52141 agtttctttt gctaaattca ttttgagttt aggctggggt ggtgcaggtc tttatggctt
    52201 cttcaaccgt ttactgatcc ctacaggatt gcatcaagca atgaactccg tgttctggtt
    52261 tgacgtagca ggaatcaatg atattggtaa atttttggct tctgaaggaa caaaaggcgt
    52321 aacaggaatg tatcaagctg gatttttccc aatcatgatg ttcgggcttc cagcaggagc
    52381 atatgctatt tacagaaatg cacgtccaga aaacaaagca aaaacagctt ccttgatgct
    52441 agcagcagct tttgcttcat tctttactgg tgtgacagaa ccacttgaat tttcatttat
    52501 gttcgttgct tggccattat atgtcttgca tgcaattttc actgggattt ctttagcagt
    52561 gagtgcattc ttccattgga cagcaggatt tgcctttagt gccggatttg tcgattactt
    52621 cctatctttg aaaaatccga ttgcgaatca gccattgatg ttgatcgtcc aaggattagt
    52681 aacagcagtg atttactact ttggctttaa ctttgctatc aaaaaattcc atctgatgac
    52741 tcctggccgc gaggaagcag atctttcaga tgacgatact gcaacgacta attcagacaa
    52801 taaatatgcg gcacaagcaa gcaaaatcta cgctgcatta ggcggagccg acaatgtaac
    52861 ttcgatcgat aactgtacta cacgccttcg tttgcaagtc aaagatacag gcctgatcga
    52921 tcaaaataaa atcaaagcca ctggcgtacc tggtatgaag atcatcgacg gaaaaaatgc
    52981 gcaagtcatc gtaggtacag aagtacaatt cgttgcagat gagatggcaa aactacatgg
    53041 tggtgctgca gcacgacctg cacaaacaaa caccgtggtt aaaacagaaa catttgccgg
    53101 tgaaacgatc gaacaagata tttacgctat cgcaaatggg aaactgattc caatcactga
    53161 agtttcagat gatgtattcg cggaaaaaat gatgggtgac ggctatgctg tattaccaga
    53221 aaatggagaa atcttctctc caatcgctgg tacgatcaca aacattttcc caacgaaaca
    53281 tgctgtcgga atccaaacag atgctggtat cgaagtcttg cttcatatgg gcatcaacac
    53341 tgttgactta aaaggcgaac cattcacttt atatgtagaa gaaggtcaaa aagtcgcaag
    53401 aggtcaattg attgctttag ttgatcttgc cgctattcaa tctgcaggca aaaacacgga
    53461 tatggtcgtt gtattcacaa atggcgacaa agtccaaagc ttagaaatcg aacccgcacg
    53521 tgatgtaaaa gcaaatgata aaatcggctc tgtttccaac aaagcctgag ccatcgtctt
    53581 ccccagactt ttttatcgat aaacaaacag gagctgagac aaaaatcttg tctcagctcc
    53641 tttttccgaa taaaaacggc gttctcctgc caactccgcg gtaataagtc cagctaaacg
    53701 aaacatatga agagctattt tttatttgac tcttcttatt acttgatgca gaacagcttt
    53761 ttcacaaaat ttttcttcat actcaaaact aatcggcttt ttcacaacct ctttatacat
    53821 tattcctgat ccaatatttc ttttaatgct gcttcaagag aagttaatgg ttttcccaat
    53881 actttttcta aatcatcaga ggaaatagct aactgatcct ttttaatatc atgttggatc
    53941 gctaaaaaca tgtcagctgc ttcttgaggg aatccggctt cttttagatt ttcgacaaac
    54001 gctgcatcac ttgcttcaat aatttctaac tctttacctg tcgcacgctc taatgccttg
    54061 gctaattctt catagtcagt tagattgccg gataattcta agatttctgg agagtcactt
    54121 tctactagta cttttgctgc agcttctgca tattcacgtt tcaatgccca acctgttttt
    54181 ccatttcctg cagcataaac gaattgacca gtttttaaag cctgcccaat gataggtaat
    54241 tcattctcta cataccaatt attccgtaaa aatgtatggg aaatacctga atccttaatc
    54301 attttttcag tgaattggtg atctggcgct aatacactga tcgagtgatc ggcatctgca
    54361 aaacttgtat aagcgatata agatacccca gctgcttgtg ctgccttcac tacattctta
    54421 tgttcggctt gacgatttcc tggaacgcca gagacaaaaa gcaatcgatc tattcctttc
    54481 aacgcttctt tcattgagtc taaatcatca taatcaccaa tacgtatatt aaatccagct
    54541 tctttcagtg cttctccttt ttcttcacta cgtactagta catagatttc attttgtgga
    54601 accatctttt ttaagaattc cagcgcataa ccaccaaaac ctccagttgc tcctgttact
    54661 aaataatgca tatttgccac tccctattct ttttatttgt taagatttac agctgtacat
    54721 ttataaatta cacctttatc ataaagagtg tggtaagata agtcaagcaa aaaaaatgaa
    54781 aggatgacaa ctgatgaagt attctctgca atttagtgat gcaatccatg tacttgccta
    54841 tattgaaatg tttcaagata cccgatcact ttctagtgag atgattgcca gtagtatcga
    54901 aacaaatgca gcaaatgtac gtaaaatcat gagtcagctc aaaaaatccg gtttgatctt
    54961 tacaaaaacc ggaaaaccgc agccttccct tgccaaaagt ccagatcaga tttctttact
    55021 agatgtctat cgcagcatcg aaggtaatac acagcttatc caagtcgatc caaaaacaaa
    55081 tccaaattgc gtcgttggtg cgaatatcca agaagcttta gaagaaagct acgctcggct
    55141 gcagaaaaag gcagaagaag aaatggctaa gatcacactt gatagtctga tacataaaat
    55201 agccgttttg gaaaaagaaa aaagaccaga aaacacaggc ctcgtagaga gatttttata
    55261 attctctcta ctgtgcctgc atttttctga tcggaatcgt ctacataaat tcttttattc
    55321 tccgggggac aaaagccttg tcatgcccct ttttcctctc taatgctcag gtcaaagcgc
    55381 tgaagtcatg acgtcttgat aaacggtgtt caagtagctg accctcggca ttagttctga
    55441 aaaatgcaaa atatggaaag ctatttccca tttttcctct ctaatgctca ggtcaaagcg
    55501 ctactttcac gacctctata ttatcatggt ttattgacta ccggtatcca gatttctccc
    55561 atatatttcc cgttggtaac tcccatttcg actacagcat ttggaccccc aacataggcg
    55621 taatcttcta cttctcttaa aacatgccca aaggccgtat tcgtcaaatc atttcttaat
    55681 tcttcaggcg attctgctaa agcgcttaca acaagatacg ttccttttgg gaatgggatg
    55741 attcgcgtag tgtttggaac atcctgactc gtttgaacac cgacatagta catcattttc
    55801 ccgttatagg gttcgttgac tgtaaagaaa taatgatttt cagcagcttg tttcaatgca
    55861 tctattgttc cattaccgat aactttttgc tcaaatgctt gtttctctgc agccaaaccg
    55921 acaaaatccg tattgctact cttcaattcc acaccaatac ccgtcaccgt aaaatctggt
    55981 ttttcttcga tcctatactt tgccatttct cccacccttt ctattatatt aagttcatct
    56041 tacataaaat taaagggaaa tgacacgatt acgctctttt ttctagggat atttttattt
    56101 tttaactatt tttttgcttt cactgcaatc caaatctcac tgtaaacatt actgaaatcc
    56161 tctccccatt ttgtaaaaga gatctcggga gcttcgacta acccataatc tgaagaagga
    56221 agccattctg catagatttt cccccaagta tcttgtaaag tttggggaaa tggcccttta
    56281 ttaggaaaaa tggcccaagt gtgttcttct atagcaagca tttctaaatc agcgtacgga
    56341 ttttcttttg tcgtagcaaa gccaatcatg tgcatcaaac tgcccttttc ttccaagcgg
    56401 tctccatcaa atccataaga aacgttgatt acttgatttg gatataaatc tcctaactta
    56461 tgcatctctt ccctttgagt cttcgtgatc gtctgcgcta acttttgtat ttcttgattg
    56521 actccttcaa atttgacagg aacttttttc gccacacctg ccacataaaa ctgttctttc
    56581 ttttcgatac gaaattccat cgattttcct cctctgacat caataaaaaa tgtcaagcgt
    56641 ggaaaagatt tttgtatgcc gtatctactg atttctgaag gcagatatcc tgaccaatca
    56701 cgaaaagctc ttgaaaaacc ttcgactgtt tcatatccat atctaaacgc cgtttctgtc
    56761 actcttgatc catctagaag atcgcgatta gccaaagcca atctacggtt tttgatatag
    56821 tccattagcg acatccctgc aataaacgta aagatgcgtt ttaattgata ttcagagatt
    56881 cctgtatttt cggctattat tttataggaa agtccttctg tcaaatgttc ttcgatgtaa
    56941 tcgatcaatt ggttcagatc tttaagcata tttatcttcc tccttgattg cctttttatc
    57001 atattatcaa agaaagtcaa ttacttgatg aatcaggaac caatttagtc agatgtagaa
    57061 aatgttgtat ggatctaaag aaaattctca agcagcgttt cgtaacggat aattggtatt
    57121 atctgcctat ctatgtttta cctttagaaa aataaatttt ggggaagaca gattctaagt
    57181 aaacaaagcg gtaaaaatat cttttattcc cttagttaat ccgcttacta cctactgtac
    57241 aaagcaaatt gttgttcaaa ctgctgatca gaacgctgct tggatttttc tttgcgatac
    57301 atcatacctc ttttatcaat tggaacaatt atttatttct aacaaaatca cttcattttt
    57361 gtcagataaa taaaaaacaa aaaaagttcc atctttaaaa cacgctattc ttttactttt
    57421 tctcacattc tctaagaaaa gaaaaggaga atgaattttt gacaattcga gcaaaagtga
    57481 ttatcaaata tgtaaaatca gctacttttt tcttaaacgt ttctttatac taaatataaa
    57541 taagtgtttc atgaaaggag aactagatga ataattggct tgagtatttt cctgaaaacg
    57601 ttttggaacg cggttacagc tatcatctgc acggttttgt ccgtcatttg aactatacaa
    57661 gcaagtactt gtccgcaaca gtttctggca cggaagatta taaggtagtg atcacttggg
    57721 atgaaaagac caacatgact tgtgattgtc tatatgccat agaaggaaaa aaatgcaaac
    57781 acatggctgc tgtcttattc gcttacgaag aacgtccaat aaaaaaatcc aactactcac
    57841 tttccgaact ctcgtccctt gtttcctccg ctagttcctc tcttgtacgg gagcttttaa
    57901 cagagattct tatagaacat ccgcacttta tcgaacgctt caaagttaag atgccatttc
    57961 atgcaataaa ttattcagat aaattaacaa ctatcatcca taaatacgat catattatca
    58021 agaaaaacaa aaacaggaaa acagcgaagt tcatcatgga gatgcgaaag ttcatccaag
    58081 aagcagttga atcgcttatt cagcaaaatg cctatcttcc cgcattcgaa ttgatcaatg
    58141 aagtgattgc tactctcgaa acattctatt gggagcctga ggatgaacga acccttcttc
    58201 tgatagagga ctgttattat ttatggaaag aattattagc agaagcacct catgcagaaa
    58261 aaaggcaaat gttctcttgg ttcgtctgcc aagtcgatca tactgacgcc tcttattcaa
    58321 aacgctactc tataaaaatc ctgaaagaag actttcggga aaaagaattt tctaaccaaa
    58381 agaaaaaaat agataaaaaa ctaaagaaac ggtaaagaaa gacgatttca atgaaaaata
    58441 gcaagtaaac gataacaacc atcctaaatc cacaagaaat acagcagacc ttgctttaaa
    58501 aacacgaatc atgcaaagga tgaagtaaag aagacttaac gcgtacccaa tattagtcat
    58561 ttgactatta ggtacgcgtt ttgttttata agtcatgaca aatttccttc gttgctggat
    58621 agattttccg gcattgttga tagattttat gatacttttc tacattttgt aagagaggtt
    58681 ccgctgcatc tgtatattgg ataaaattct gacaacaact ttcaggtgtg tcgaaccacc
    58741 ctaaccctag tgcagcaatc attgttgcgc ctaatgcagg tccttcctcc acacttaaac
    58801 aatagaccgt tgcatcgaaa atatcagctt ggatttgtag ccatgcaaga tttttcgcgc
    58861 ctcctcccac cgaaacaata cgtgtaaatc tgcgacccgc tagttcttta cgtttcaatc
    58921 ccaccattga caaatgctca ttaaaaattt agaatattca caagtttaac gacaattatg
    58981 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
    59041 cgatcaaaga cacgttacct accgttttcg gttatatcgg tattggactt gcatttggta
    59101 tcgttgggaa agctgccgga tttcatccac tagtcgtcac gttgatgtcc ctgctggtct
    59161 atgctggttc tgcccaattt atcacagtca gcatgcttgc tagtcacagc ccattgcttt
    59221 ccatcgtttt ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
    59281 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
    59341 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
    59401 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
    59461 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
    59521 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
    59581 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
    59641 tcccaagcaa cttagtgatt gtagttgtaa cactgattgg gtgcggattg ggggtgtgga
    59701 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
    59761 tggctttcta gggtcctacc atttgtctta ctgaaaaaat tcgatttacc gcaaccgttg
    59821 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
    59881 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
    59941 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggcaggaatt
    60001 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
    60061 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
    60121 atccgttgaa cggaccacta acaacgactt ttcctaacat atgtcctgtt tctacgtctt
    60181 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
    60241 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
    60301 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
    60361 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
    60421 caaatggtcg gacaaggtgc ttgatttgat caaagtagcg ggtaatatcg aataatacag
    60481 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
    60541 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
    60601 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
    60661 cggatccgtt gatcactaag atttttttcc cgcgattggc attttcttct ggagttagac
    60721 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcagctt
    60781 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaact agttggtatt
    60841 cttgattgct tcctgctctt gttgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
    60901 atttttgcac ttcgtcacct acagcaacaa ctttcccgac tccatcaaaa cctaagatac
    60961 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
    61021 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
    61081 caataaagct ttttggatca tcgatcggca acccttcata aaaaccgact accttcatta
    61141 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
    61201 gttcatttca atatatatag tatgctattt gttccacctt ttttattcat cataattatt
    61261 ctcttactta aagtaaacta tttgcacttt tttggttaaa aaagaggttg cgacaagact
    61321 tttgtcacaa cctcttataa acggtgttct cctaccaact cttcggtcac aagtcaaaat
    61381 attcatcaaa catgagaagc tgttttttga aattgctcct tgtgactcag agttaaacgg
    61441 caggctcaca acctctgata taaacggtgt tcaatcaact gaccctcggt attagctcaa
    61501 aaaacgacaa aatatgaaga gctatttttg tttggctgtt cttattactt gatacaggac
    61561 agctttttca cgacctctaa ttcacggtgt tcaagcaaga aatcattgtt cactgaaaag
    61621 cgttggatgt tctttttcaa aggtctctaa gatctctcgt acttcatcag tagatttcgt
    61681 attcatcaac tgaactcgca agtcatttgc tcctggaaat cctttgatat agattttaaa
    61741 gaaacgatgt agtcctgtga tggaacgcgg gatgatttct gaataatgat cttgcagatc
    61801 tagttgcaga cgaagtagtc cgatcagttc tttttctgta tgttccctcg ggtctttttc
    61861 aaatgcataa ggattcttga aaatccctcg gccaatcatg atgccatcaa cgccatattt
    61921 ctcggccaat tcttctcctg tctggcgatc ggggatatct ccattgatcg tgattagtgt
    61981 ttgtggagcg atttggtcac gtaaagcaat gattttagga atgacttccc aatgtgcatc
    62041 tactttactc atctctttac gcgtacgcaa atggacagaa agattggcta tatcttgatg
    62101 aagtaggtga ctgatccatt cttccatctc agacatttcc gtatagccga tacgcgtctt
    62161 gacgctgaca ggcaaaccgc cagctttagc tgcttcaatc aattgtgccg ctacttctgg
    62221 acgaaggatc aagccactgc cttttccacg ttctgctaca ttagggactg ggcagcccat
    62281 gttgatatcg attccttgga agcccatttc tgccatagca atgctcattt ctctaaaaaa
    62341 ttctggcttg tctccccaaa tatgggcaac catcggctgt tcatcttctg taaatgttaa
    62401 tcttcctcgt acactgtctt ttccatctgg atgacagaaa ctgtctgagt ttgtaaactc
    62461 agtaaagaag acatccggtg ctgctgcatg ctttacgaca tggcgaaata caacatctgt
    62521 tacatcttcc attggtgcta aaacaaaaaa aggctttggc agctctttcc aaaaattagt
    62581 agtcatttat ttgttactct ccattctatc aagcagtgaa gtcgcaatct ttctcaaaaa
    62641 cattgtcatt atactaggaa acaaaaaaag aagcaaagtg aacagcttaa aagatgtttc
    62701 tgattgagca aaaaaaagac tgtgacaaaa atcttatcac agtccatttt ccggaataaa
    62761 tggtgttcaa aagcttgcat ttgcggtaat aagtccaacc aaacgaaaaa taggaggagc
    62821 tatttttgtt tggctgttct tattacttga tgcagaacag ctttttcaca acctctccac
    62881 taaacggtgt tcaatcagct gaccctcggt attcgctcaa aaaacaacaa aatatgagga
    62941 actattttcc cgtttgtttc tctaatactc aggtcaaaac gctgatttca caacctcttt
    63001 tctaacttct caatcctctt cctttattga tcaaatacca agtaacggcg tacaaaacaa
    63061 cgatgaataa cacaagtacg atcatcgatg ttgctactgg cacatcgatc gttccaagga
    63121 atccgtaacg gaaaccggag atcatataaa cgatcggatt gacttttgaa acagcttgcc
    63181 aaaagggcgg taacatagag atggcataga aaacgccacc tagataagtt aacggctgca
    63241 agacaaatgt aggcacgatc gatacatcat cataagactg agcaaaaatc ccgttgagca
    63301 gtcctgctaa tgaaaacaag attgccgtca tcaataatgt aatgatgacc atgaaccatg
    63361 aatacacatg aagtggtaca aagaacaagg aaatgatcgt tacgagagag ccgacgagaa
    63421 tactgcgacc cacaccacca atcacaaagc cccaaataat cacatgcgta ggaacaggtg
    63481 ctaccaagat ttcttcgata tttttctgaa atttttgaga gaagaaggac gaagaaacat
    63541 tggcatacga actagtgata gcagacatca taatcaaacc aggaacgatg aattccatat
    63601 atgaaaaacc acccatgtca ccgatccgcc caccgatcat ttttccgaaa ataacaaaat
    63661 acaaggatgt cgtgatcact ggcggaacca gtgtttggac ccatatacgc aaatagcggt
    63721 tcgtttcctt gatagctaaa ctttttaatg cagtaaaata aagattaaac atttttctcc
    63781 tccgtatgat tattttcttc tgtgattttc aagaacagtt cttctaaacg attcgactta
    63841 ttcctcatag aaagaacttt gattccttgt cttgtaagct gatcgaagat tccgttcacc
    63901 ccttgatttc gttcaacttc gacagcgatt gtctgctcat cttcaaatgt acaagcatat
    63961 ccttcaatca caggtttggt atcatatggc gctaaatcaa agataaatgt ttcaaattgc
    64021 agttttgaca gaagctcttt catacttgta ttctcgatca gttcacccga ttggatgatt
    64081 ccgatgttgc gacaaagcat ttcagcttct tctagataat gcgtcgtcaa aatgatcgtt
    64141 gtaccatttt cgttcaattc tcgtagaaac tcccacatct cgcgtctcaa ctcgatgtca
    64201 actcctgctg ttggttcatc caagatcagc aaacgtggtt catgcatcaa tgccctagca
    64261 atcatcagtc ttcgcttcat tcccccggaa agcatacgtg cacggacatt tctcttttcc
    64321 caaagattgg actgcttcaa atatttttcg ctgcgtttca tcgcttcttt gcgagagaca
    64381 ccgtagtagc ctgcttgatt cacgacgatt tgctggaccg tttcaaaagg gttaaaattg
    64441 aactcttgtg gaaccaatcc aatctgttgc tttgctcgaa ctagatcctt gtccagatca
    64501 taaccgaaga cattcacttt tccagatgtt ttattgacta aagaagtaat aatcccgatc
    64561 gttgtagact tcccagctcc atttggccct aatagcgcat agaaatctcc ttctttcacc
    64621 gaaagatcga ttccccgcaa tgcttcgacc ccacttccat agatcttttt caaattattt
    64681 atttcaagcg catttgccat ttatttccta tctccttata gacatgattc ttaattgtat
    64741 catggaatac agcagatgaa aaaacaactt ggcttccaca caaaaacaca cctgctaaca
    64801 aagaggagat gtgttttcta gttcatgatc atcttcgata gaataactat ctaaacaact
    64861 ctggaacttt taaaatcata aaagtaccga cagcaactac tacgatgaca tatagaagat
    64921 ccttataact gaggttcttg tcttttttat tcatgttaag ctttcctccg tccttttatc
    64981 gaaagctttt ttcttttatt ttaaccgaaa taaataaaat ggaaatagtt attttcaatt
    65041 tctgttttcg ctgtttgttt tacggagaac tttgccatgg aacgtgcgtc tcgtcgctga
    65101 tgctaagttt tttcgataaa agctgctaga aggaagacag cgtggtttat tttgtatcgt
    65161 cttttgctaa atcttccaag atcatctgga cagcagactc gttcccattg aattctgcca
    65221 cttttttggc atcaatgtac ttcttttgat aatcgtcttt ttggacccac gcatcatgct
    65281 tcgtttgata aagttaatca aacggatacc actgacgctg ttcatcgaac aacaaacttt
    65341 ctttttgggc ctgttcaact atggatttag gataattcaa aactttcagc aaggcaatcg
    65401 catttcgggt ccttgaaggt ccttctttta atttgaaatc aaaactgatt ccttttcctt
    65461 ctgttacttg ttcttcaaag tggacattct cacaataatt ctttaaaatc tctgtcaatt
    65521 cgatatcatg tgtggcgaca aatgctaaag atggatactc cgcaagccac cgaacgactg
    65581 aagcggaaga agcaatccgt tcgatcgtat ttgttccttt taaaatctca tctacgaagc
    65641 agtagcagcg ttctttggaa gccacttggt ccagcaaacg tttgatcgat ttgatttctg
    65701 aaacaaaata actgtcgcct tcaaataaat catcttcgat tgccatggaa gtcaacacat
    65761 gtccaggttg cagggtaaac tgttctgcaa tcgctgtttg gatcgtctgt gctagaatac
    65821 agctgatggc tatacttttg acatacgtag attttcctga tgcattggaa cccgtgacta
    65881 atgtattctt ctgccagttg actggattaa ctaccgcttc ttttaacaat ggatgataga
    65941 tatctttcgc tgtcacaccg ccctcagtaa actctggtcg gcaagtgatc ggcatatagg
    66001 tgcgataatt caaaacggct gctgcaactt ccaattctcc cagcaattcc cacagcttga
    66061 tggcttcagt tgaatgcttt tctaatttag tgagaacgaa atgataggag ataaatggca
    66121 gcatgaacat gatgttgaca tattcaaaaa tcaattctgc ttcactcccg ctcttcattc
    66181 gaaatgaaaa acctaattta gggatgtctc ttaatggttt agtttgctgt ttcagctctt
    66241 tttgaagtgg tgtcgtcatt tttgctaact gattggcggc tgaaatcgtt gaaaccaaat
    66301 aactcatgct attcagctct gtttctagtg tttgtttctt cactagataa tagaccgtat
    66361 tgaatactaa gccaccgatt gctagataga ttcctagtac ttgccctaaa agaagcaaac
    66421 ctacacccac cagcggaagc aagccaagaa aaataaataa acctacgcgt ccaatctttt
    66481 tactttttcc atccgctaga tattgttttg caaaattatg atcttttttt cctaaacgag
    66541 cgaattgata ttggattctt tctctcagtt gaggattttc ctgataaaaa gcaattagtt
    66601 tttctaactg ataatcttct ccaaaatcaa agctacgtaa acgctgatac aacgcttctg
    66661 aacctacact agaataggta ctgttgatcc cttcaaatac ttcgaacata tccaagtcat
    66721 accaagtaag atcgtcgatt tcactatccc atgaacggaa ttttttctcc gtgagccacg
    66781 catccttcaa actttcttct ttatcaaatc tcggttgata aggaattttt ccccaattac
    66841 tacgaactgt ttcctttact ttcaaacgtg aaaaaatgtc taaaacaact aataaaataa
    66901 caactgcaat aataattaaa acgataaatt ctctttggcc catatccctc tccgttcctt
    66961 gatttgttag ttttagtata gcactgttgc gctatttgaa aagggggtac aaaaataact
    67021 gtcttcgtta cctaattcgt atcaatcaca ccaaaataaa aacaccttcc aaaaaagaag
    67081 atgtctttaa aacgagatac aattttttgt cattcacttc aggtaaatta gagccaatta
    67141 aaccctccgc aacttgacgg agatggagaa gatgcccccg tctaatctag cggtcatctc
    67201 tcctcccatt ccttccacca atttttttga caggtaaagc ccgattccgc tagattccgt
    67261 atttgtgcgg ctggtatttt cggtataaaa acgctggaag actttttcaa cagaggtttg
    67321 cgctcctggt ttgataggat tggccactgt taatcgaatg aatgaatcct cttttgaaaa
    67381 tgtcaccttt gcttgtccat ctgcataacg caataaattc tgtatgatat tttgcagcac
    67441 tcgcagcaac atgagcggat ctgctaatac agttgtattt atcgagtgtt cagcgaacgc
    67501 tacctcgata tttttctcag aaaattgttc ataaaaagag aaaaatactt cttccacctc
    67561 tttagaaaaa gaaagcgtat ggagatccat ttctttttgc ttcgtctcta ttcgagcaag
    67621 atcataaaac tgatccgtca tactgagtaa tcttgcaaca gattgtttga tgatcgttaa
    67681 atacttctcc cgtttctccg tatcctctac ctgttccagt aattgtacat aaccgttaat
    67741 agcagtcaaa ggagttctga gatcatgaga aaggccgacg atcgatagtc tgatgttttc
    67801 ttccatttct tgaaatgctt gtcctttttg ttcattttca tccacccatg tattcagtgc
    67861 atctactagg tccattagat tcttttctcg aaattcgact gaaagacgac caccatattc
    67921 ttgtcgttca gaaagagtgg cgattttctt agttaatctc ttgatttccc tttgcaggaa
    67981 gaaaataaaa cttcccataa agataacaac gagaagcaac agccacttaa tcaaatctaa
    68041 accccactcc ccacaccgta tgaatataca cagcatgtgc ctttgctagt ttccctcgaa
    68101 gattggaaat atgcatattc actgttttct cactatcaaa atatggttct tgccaaactg
    68161 tttcataaag atttgctcgc gaaaatactt ttttcggatg ttctaaaaaa agcttcagca
    68221 gatcgaattc tcttcctgtc aaatggacag ccacaccatt cacatttaca tccctcgact
    68281 ctttgtccag taagatttcc ttgtaataga tgcgttgcga cagatctttt acttggacag
    68341 aagtctgatg acgaagttgg atgccgattc gtaacaacag ttcttcgata tcaaatggtt
    68401 tcgtcacata gtcatccgct cctgctgcca acaaggaaac tttatctctt tgatcgcttt
    68461 ttgctgaaag aacgatgatc ggaacattcg atgtctgtcg gatttctgct aacaatgctt
    68521 cacctgaaag ccctggaagc atgagatcta gaaggatcaa atcgacagga tatgtgctta
    68581 acaataattt tccttccgta cctgaataag catcaagaag tttgtatctc tgttcaagaa
    68641 cctcctccag taaagaatga acagcttcat cgtcttcgat aatcaaaata gtttccagca
    68701 ggaacaccta cttaatgtca gatttttgga aaataaacat tcctagagct gatgatacca
    68761 cgattgttac tagactgacc agaatcattt tcggtgcttg cgaataagtt gcttgttgcg
    68821 caaagtccgt atacatgagc cacgtttccg taaaccactc cgttagaaat tccaagcctt
    68881 tgatttttgc gaaaacaccg ggaacgaagg ccgttcccat gatataaaga aaagaaagaa
    68941 gcaaactgac actggtactt ctagtgatga atgtcaaagc cactggaaca gataatgtcg
    69001 ctaatatcag cagaagcaat acaggaaata cttttagaac ataatttcca tagtttttga
    69061 ttccctcaac ttcaccaaat ccgaaagcaa gtgtgtaagc aatcatggct agccccgtaa
    69121 tcaaaaccac gccaagtgtt gtgatcaaaa gcgtaacgag aagtttcgac acataataag
    69181 tcattcgatt ccgtcctaaa gataacgtat tgcgaatcgt accgttttga aattcttccc
    69241 caatataaaa gcacgcaaat gctgcgaaag ccagaagaat ccatgcagcc attttttcca
    69301 ccattaaaat ccctgttgca ccgttgatga ctgtatccgt tgcttggttt aattcattgc
    69361 cgtattttgt atgcatatac cattcgttcc aagaataaaa aagcggaagt acgaccatca
    69421 gtcctagtga gacccacatt ttacggctct tccatacttt tattttttca gcagttaata
    69481 gattaaacat gtggattgcc tccagttaat tgtaagaaat aatcttccag tttttgccca
    69541 actaggtgga taccatccac aggaactttt gcctctacca atgcttgatt gatggctgct
    69601 acttggtcaa ttccttcaaa aagctggata acacggctag attggacaaa ataatcccta
    69661 taaccaagag agtcgagaac cgttactgct tgttccgtcg ccgaagtgtc aatttttata
    69721 aattgacgag attcttgtgc caattcttct ttcgataatt ctttgatcaa gtgtccttca
    69781 tgcaaaatac cgtagtgagt ggcgatctgt gacaattcat ctagcaagtg gctagaaagc
    69841 aagacggtga tgccccgttc ttttaccaaa cgttggatga tttctcgcat ttctgccatc
    69901 cctgctggat ccatcccatt cgtcggttca tccaaaatca aaaattctgg attcgtgatc
    69961 agtgcatttg caatagctaa acgctggcgc atacctaaag agaaattctt tgctttcttt
    70021 ttccctgtat tttccaatcg catcattttc agcaggtctt cgatttctct atccgacaca
    70081 ccgccgttcg ctgcttggat acgtaggtta tctcttgcag ttaattctgg ataaagagca
    70141 ggggtttcaa tcgattgacc catacgtcgt cttgctgcct gcaaatccgt cgttccaaat
    70201 aattcgatcg aaccttcatc aatcgagatc aaacccataa tagttctcat aaaggtagac
    70261 ttacccgctc cattctcccc aatcaaacca tagatcattc cacgtttgat ttcgatcgat
    70321 actttatcta atgctttggc agcaccgtat tttttggtta taccagttgc tttcaagacc
    70381 gtttcatttc caaaatccat acttatcctt ctttcttatt gataagtgaa gtatagttga
    70441 cgggtataaa gaaagcagcc agaaatgtat aaagaaagtg taaattttcc aattgaattt
    70501 caagaaaaat cagactgtct cgcagacgtg gttgagttgt ctgccaatca aagagcgcac
    70561 caaacagtgc taatccaagt ggactggcca ccatggtaat cgttgttatc gttgacatca
    70621 gacgtccttg tatatgcgtt ggaacttctc gctgaagcca gaccatataa aaagcaaaag
    70681 aaaacattaa tccttaaaaa cggacttatg aaaaataata catgccgttt gggatttaca
    70741 attagctacc aaataagagg ttatgacaaa agacttttca caatcccttc ttccgaataa
    70801 acggtgttct cctgccaact cctcggtcac aagtcacaat atttatcaaa catgagaagc
    70861 aatttttgta aattccttct tatttcttga agctgaccac ttctgtcaca acctcagaag
    70921 cggataaata atattgtttg atgatacttt atctttttcc cgtttgttta tagatcgaat
    70981 gatattatag accaagaatc ctacctatta acatagctaa aaacacaaaa aaggcacttt
    71041 ccaaattgtg gaaagtgcca gaaaagttag gattaaggta actgcgctca gcattgcttc
    71101 gcccagtacg attctcgtaa ctcgtgaact cgaacgataa tcgcaattaa cgttttgaga
    71161 actgtgaagc tttacgggct ttcttaagac ctggttaaac aaaactctat tttattgcat
    71221 tttacaggaa tgtatttcat gcgaaattaa taagtctttg tttatagagt tttactaggg
    71281 tataaaaagt tgtatgttat cattacctca aaacattacc tcagcttggc gagttggttg
    71341 attgaagaat aaatcattta tctagatatc tcaataaaaa atgatttcta agaaatttgt
    71401 attttcgtgt tattattttt ttgccctatc aaaatttcta attcttctag cgtaatatga
    71461 ttatgacaca taatcgattc aattacttca atacctattt ttttcgagaa agatcccttt
    71521 tgttcactca caaaaatact ttcagaagac atatataagg aacgaagttt tggaattagt
    71581 atatcgtaca aatagaccac cataaaatcg tccttgcaac cattctcaaa aagaattaaa
    71641 tttgctgatt gtgttacata tatcactgaa ttacattcat ggtcaattcc aaaaacaatg
    71701 tctacttcat tttcttttct aattcgcgca gttacatttt tcaaactttc accattactt
    71761 ataaatgctt tagttactgt aagctctgat gtttgttcag atattccttt tataatattt
    71821 tgttcatcag ccacattccc cataaatccg ccgattcctt ttaagtaata attctcacct
    71881 aagagtcgct tgcattcact gaattgataa aaaaaccagt taaaaaaatc ttctggaaaa
    71941 ataaattctg atcgaacttt atttatattt ttagctccta ttaattttat aactcgatta
    72001 atgttcactt gctgcgatga acatataact gaataaattg agttgtttaa ttcaaaaaaa
    72061 caactttaac atatgcatca ttaattcgtt attttttagg tagatattga tcattgttat
    72121 ctctcttttt ttgcttccca tactccagct tagaaattgc cgttataaaa tttattttaa
    72181 aggtgacttt ttcccctgaa gtatcggtag taggaatact taatacctca gcttctgagg
    72241 aggtatccca attatagtta tactttgcat catcaccata aagaacaatt ccattttttt
    72301 atcttgtgcc gttagcttta ctggttcgct tacttttctt gttaatatct tggactttac
    72361 atcatcgata ttgcttattt tatcaagctt aaataattta caaattaatt tcgtactagt
    72421 ttttatattt gacgagagtg taaaatattt tgtgtaaata gaaaaaagga agtcccttct
    72481 gtagaataga gttaccacaa cacattcaca gaaaagagga cttccatatg aacgatttta
    72541 ctacagaaat tctaaagact ctagcgaaca aaggcgattt gaatgaatta ttccgtgtcc
    72601 atttggaaaa agctgtcaat acgcttctca aaacggagtt aacggctttc ctcgattacg
    72661 aaaagtacga tcgcattggt tttaacacgg gtaattctcg taacggctcc tatgaccgta
    72721 cggtcaagac cgagtacggg gaacttcatc tccagattcc gcgcgaccgc aacggcgagt
    72781 tcaagcaaca gactgttcct gcttatagac ggacgaatga tac
//
LOCUS       DACDYJ010000005        61959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDYJ010000005 DACDYJ010000000
VERSION     DACDYJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.005"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            148..1293
                     /gene="tgt"
                     /locus_tag="IWM51_000331"
     CDS             148..1293
                     /gene="tgt"
                     /locus_tag="IWM51_000331"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP9969778.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1390..1770
                     /gene="yajC"
                     /locus_tag="IWM51_000332"
     CDS             1390..1770
                     /gene="yajC"
                     /locus_tag="IWM51_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP9969779.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            2075..4672
                     /gene="adhE"
                     /locus_tag="IWM51_000333"
                     /gene_synonym="adhC"
     CDS             2075..4672
                     /gene="adhE"
                     /locus_tag="IWM51_000333"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP9969780.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            4901..6007
                     /locus_tag="IWM51_000334"
     CDS             4901..6007
                     /locus_tag="IWM51_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9969781.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            6024..7052
                     /locus_tag="IWM51_000335"
     CDS             6024..7052
                     /locus_tag="IWM51_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP9969782.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            7058..7537
                     /locus_tag="IWM51_000336"
     CDS             7058..7537
                     /locus_tag="IWM51_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP9969783.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            7553..8848
                     /locus_tag="IWM51_000337"
     CDS             7553..8848
                     /locus_tag="IWM51_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP9969784.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            8859..9683
                     /locus_tag="IWM51_000338"
     CDS             8859..9683
                     /locus_tag="IWM51_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9969785.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            9848..10747
                     /locus_tag="IWM51_000339"
     CDS             9848..10747
                     /locus_tag="IWM51_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9969786.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(10873..11742)
                     /locus_tag="IWM51_000340"
     CDS             complement(10873..11742)
                     /locus_tag="IWM51_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9969787.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            11895..13355
                     /gene="rhaB"
                     /locus_tag="IWM51_000341"
     CDS             11895..13355
                     /gene="rhaB"
                     /locus_tag="IWM51_000341"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP9969788.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            13352..14608
                     /gene="rhaA"
                     /locus_tag="IWM51_000342"
     CDS             13352..14608
                     /gene="rhaA"
                     /locus_tag="IWM51_000342"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP9969789.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            14622..15467
                     /gene="rhaD"
                     /locus_tag="IWM51_000343"
     CDS             14622..15467
                     /gene="rhaD"
                     /locus_tag="IWM51_000343"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP9969790.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            15469..15780
                     /gene="rhaM"
                     /locus_tag="IWM51_000344"
     CDS             15469..15780
                     /gene="rhaM"
                     /locus_tag="IWM51_000344"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP9969791.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            15862..17013
                     /gene="fucO"
                     /locus_tag="IWM51_000345"
     CDS             15862..17013
                     /gene="fucO"
                     /locus_tag="IWM51_000345"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP9969792.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            17140..17898
                     /locus_tag="IWM51_000346"
     CDS             17140..17898
                     /locus_tag="IWM51_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP9969793.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            17998..18642
                     /locus_tag="IWM51_000347"
     CDS             17998..18642
                     /locus_tag="IWM51_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9969794.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            18649..19656
                     /locus_tag="IWM51_000348"
     CDS             18649..19656
                     /locus_tag="IWM51_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP9969795.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            19672..20514
                     /gene="kduI"
                     /locus_tag="IWM51_000349"
     CDS             19672..20514
                     /gene="kduI"
                     /locus_tag="IWM51_000349"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP9969796.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            20534..21337
                     /locus_tag="IWM51_000350"
     CDS             20534..21337
                     /locus_tag="IWM51_000350"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP9969797.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(21586..22650)
                     /locus_tag="IWM51_000351"
     CDS             complement(21586..22650)
                     /locus_tag="IWM51_000351"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP9969798.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(22647..23732)
                     /locus_tag="IWM51_000352"
     CDS             complement(22647..23732)
                     /locus_tag="IWM51_000352"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP9969799.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(23745..24722)
                     /gene="mvaD"
                     /locus_tag="IWM51_000353"
     CDS             complement(23745..24722)
                     /gene="mvaD"
                     /locus_tag="IWM51_000353"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP9969800.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(24715..25659)
                     /gene="mvk"
                     /locus_tag="IWM51_000354"
     CDS             complement(24715..25659)
                     /gene="mvk"
                     /locus_tag="IWM51_000354"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP9969801.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            25981..26634
                     /locus_tag="IWM51_000355"
     CDS             25981..26634
                     /locus_tag="IWM51_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9969802.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            26719..27624
                     /locus_tag="IWM51_000356"
     CDS             26719..27624
                     /locus_tag="IWM51_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9969803.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(27626..28258)
                     /locus_tag="IWM51_000357"
     CDS             complement(27626..28258)
                     /locus_tag="IWM51_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9969804.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            28578..29102
                     /gene="infC"
                     /locus_tag="IWM51_000358"
     CDS             28578..29102
                     /gene="infC"
                     /locus_tag="IWM51_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP9969805.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            29174..29374
                     /gene="rpmI"
                     /locus_tag="IWM51_000359"
     CDS             29174..29374
                     /gene="rpmI"
                     /locus_tag="IWM51_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP9969806.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            29427..29786
                     /gene="rplT"
                     /locus_tag="IWM51_000360"
     CDS             29427..29786
                     /gene="rplT"
                     /locus_tag="IWM51_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP9969807.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            30038..31375
                     /locus_tag="IWM51_000361"
     CDS             30038..31375
                     /locus_tag="IWM51_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP9969808.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            31423..33552
                     /locus_tag="IWM51_000362"
     CDS             31423..33552
                     /locus_tag="IWM51_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP9969809.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            33527..34216
                     /locus_tag="IWM51_000363"
     CDS             33527..34216
                     /locus_tag="IWM51_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969810.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(34624..35310)
                     /locus_tag="IWM51_000364"
     CDS             complement(34624..35310)
                     /locus_tag="IWM51_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9969811.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(35446..35640)
                     /locus_tag="IWM51_000365"
     CDS             complement(35446..35640)
                     /locus_tag="IWM51_000365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969812.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(35690..36130)
                     /locus_tag="IWM51_000366"
     CDS             complement(35690..36130)
                     /locus_tag="IWM51_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP9969813.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(36134..36979)
                     /locus_tag="IWM51_000367"
     CDS             complement(36134..36979)
                     /locus_tag="IWM51_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP9969814.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            37128..38546
                     /gene="pepV"
                     /locus_tag="IWM51_000368"
     CDS             37128..38546
                     /gene="pepV"
                     /locus_tag="IWM51_000368"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP9969815.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(38603..39550)
                     /locus_tag="IWM51_000369"
     CDS             complement(38603..39550)
                     /locus_tag="IWM51_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9969816.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(39773..40123)
                     /locus_tag="IWM51_000370"
     CDS             complement(39773..40123)
                     /locus_tag="IWM51_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9969817.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            40323..41402
                     /gene="pepA"
                     /locus_tag="IWM51_000371"
     CDS             40323..41402
                     /gene="pepA"
                     /locus_tag="IWM51_000371"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP9969818.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            41546..41866
                     /locus_tag="IWM51_000372"
     CDS             41546..41866
                     /locus_tag="IWM51_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9969819.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            41888..42352
                     /locus_tag="IWM51_000373"
     CDS             41888..42352
                     /locus_tag="IWM51_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9969820.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            42557..43162
                     /locus_tag="IWM51_000374"
     CDS             42557..43162
                     /locus_tag="IWM51_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP9969821.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(43201..44490)
                     /locus_tag="IWM51_000375"
     CDS             complement(43201..44490)
                     /locus_tag="IWM51_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9969822.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            44918..45397
                     /gene="rlmH"
                     /locus_tag="IWM51_000376"
     CDS             44918..45397
                     /gene="rlmH"
                     /locus_tag="IWM51_000376"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP9969823.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            45750..46064
                     /locus_tag="IWM51_000377"
     CDS             45750..46064
                     /locus_tag="IWM51_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9969824.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            46080..46466
                     /locus_tag="IWM51_000378"
     CDS             46080..46466
                     /locus_tag="IWM51_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9969825.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            46495..47880
                     /locus_tag="IWM51_000379"
     CDS             46495..47880
                     /locus_tag="IWM51_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9969826.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            47883..48036
                     /locus_tag="IWM51_000380"
                     /pseudo
     CDS             47883..48036
                     /locus_tag="IWM51_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            48059..49264
                     /locus_tag="IWM51_000381"
     CDS             48059..49264
                     /locus_tag="IWM51_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9969827.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            50048..51958
                     /gene="ltrA"
                     /locus_tag="IWM51_000382"
     CDS             50048..51958
                     /gene="ltrA"
                     /locus_tag="IWM51_000382"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9969828.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            52062..52286
                     /locus_tag="IWM51_000383"
     CDS             52062..52286
                     /locus_tag="IWM51_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969829.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            52403..52900
                     /locus_tag="IWM51_000384"
     CDS             52403..52900
                     /locus_tag="IWM51_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9969830.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            52987..53379
                     /locus_tag="IWM51_000385"
     CDS             52987..53379
                     /locus_tag="IWM51_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9969831.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            53363..55810
                     /locus_tag="IWM51_000386"
     CDS             53363..55810
                     /locus_tag="IWM51_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9969832.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            55813..57996
                     /locus_tag="IWM51_000387"
     CDS             55813..57996
                     /locus_tag="IWM51_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9969833.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            57993..58994
                     /locus_tag="IWM51_000388"
     CDS             57993..58994
                     /locus_tag="IWM51_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9969834.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            59009..59923
                     /locus_tag="IWM51_000389"
     CDS             59009..59923
                     /locus_tag="IWM51_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9969835.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            60168..60284
                     /locus_tag="IWM51_000390"
     CDS             60168..60284
                     /locus_tag="IWM51_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP9969836.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            60300..>61959
                     /gene="tet"
                     /locus_tag="IWM51_000391"
     CDS             60300..>61959
                     /gene="tet"
                     /locus_tag="IWM51_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HAP9969837.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSR"
BASE COUNT        19912 a        11229 c        13230 g        17588 t
ORIGIN      
        1 tttctttttt ttgttattca taaaaaaagg ataaaaaaag aatattaaat aaagtagaag
       61 gaagccgagg gaacttgcaa aaagcacttg aaacgattat gatggtggga gttgtcaaga
      121 tattttatat agaaagtagg gaaatgaatg acgcaacctg ccattcgcta tcgtttaatc
      181 aagaaagaaa aacatactgg cgcgcgttta ggggaattga tcacgccaca cggaactttc
      241 cccacaccaa tgttcatgcc agtaggaaca ttagctacag taaaaacaat gtccccagaa
      301 gagttgaaag aaatgggtgc tggcgttatt ttaagtaata catatcattt atggctacgc
      361 cctggtgaag acttagtaga agaagctggc ggactgcata aatttatgaa ctgggatcaa
      421 ccgattttaa cagattcagg cggctttcaa gttttttcat taagtgatat gcggaatatc
      481 gaagaagaag gcgtgcattt caaaaatcac ttgaacggtt cgcgtatgtt tttatcacca
      541 gaaaaagcaa tcaacatcca aaataaattg ggctctgata tcatgatgag ttttgacgaa
      601 tgtccaccat tcgatgaaag ttacgactac gtgaaaaaat cagtagaacg tacgtctcga
      661 tgggcagaac gaggattgaa agctcatgct aatccagacc gtcaaggatt atttggaatc
      721 atccaaggag cagggtttga agatcttcgt cgtcaaagcg cgaaagatct agtcgggatg
      781 gatttccctg gttactcaat cggcgggtta tctgtcgggg aacctaaatc agaaatgaat
      841 cgtgtattag aattcactac accgttgatc cccgaaaaca aaccacgtta cttgatgggt
      901 gtcggtgctg ctgattcatt gatcgatggt gttatccgtg gagtagacat gtttgactgt
      961 gtcttgccta ctcgaatcgc tcgaaatggc acatgtatga cttctcaagg ccgtctagta
     1021 gtgaaaaatg cgaaatatgc ccatgatttc cgtccaattg atgagaaatg tgattgttat
     1081 acatgtaaaa actatacacg tgcctatatc cgccatttga tcaaatgtga tgaaacattt
     1141 gggattcgtt tgacttctta ccacaattta tacttcttgt tgaatctaat gaaacaagta
     1201 cgtcaagcaa tcatggatga taatttgctt gaatttagag aagccttttt tgaagaatac
     1261 gggttcaata aagaaaacgc aaaaagcttc taaaggaaaa tcttttaaaa attaagtaaa
     1321 aaactagtgt tatcagcatt tttctgctaa agtgtttttt gtagatacag aattagtgag
     1381 gtgaaaaaaa tgggaacatt accaatgctt gtgatgttta tcgcgctatt agcaatgtgg
     1441 ttttttatgt cacgttcgca aaagaaacaa cagcaagaac gtcaaaactt gttagatagc
     1501 atgaaaccag gtgattcagt cgtaacgatc ggcggattac atggagttgt ttcagaaatc
     1561 gacaatgaca aacggacagt tgttttagac tgcgaaggga tcttccttga gtacgatcgt
     1621 gcagcaatca aaacggtcaa accagggact gtcgtaacaa atgatgcaac gactactgtt
     1681 gaaacaacag aagaaccagc tgagacagtt gaagtaaaaa ctgaaacaac agaaacgctg
     1741 gaagagacag aagagaagga agaaaaataa gtatcaaagc ttatttaaag acgaggtagg
     1801 gcagaaagat gtcctacctc attttttatt caaaaaagaa agagataaga gcatgggata
     1861 tattgctcaa tattgaataa tcgggtgtgt catataaata ataataaata actttatata
     1921 tttttctata caaacacctg taaataaagc aattttatat ttaaaaataa ctgtgaaaaa
     1981 aatcacaaaa aagtgtttac atagaaaaat aaatattgta tgatatacat gtgaattatt
     2041 taacaaataa ccatgaccca ttaggaggat aatgatggct aaagtaatag aaaaagaaaa
     2101 aacgaagaca attgacgtac aagcaatgat tgatgatttg gcagaaaaag caaatgcagc
     2161 attgaaagaa atggaaagct ttgatcaaga aaaagtagac catatcgtgc atgaaatggc
     2221 aatggcagct ttggatcaac atatgccatt agcgaagatg gctgtagagg aaacaggccg
     2281 cggtgtttac gaagataaag caatcaaaaa tatgtatgcg tccgaatata tttggcataa
     2341 tatcaaacat gataaaacag ttggtgtgat caatgaagat gtccaaaaag ggttgattga
     2401 aattgctgaa ccagtcggtg tggtttgcgg agtaactcca acaacgaatc cgacttccac
     2461 aacgattttc aaatcaatga tcgcgttgaa aacaagaaat ccaatcgttt ttgctttcca
     2521 tccaagtgcc caaaaatctt ctgcagaagc agcacgagtg gtacgtgatg ctgctatcgc
     2581 tgccggagct cctgaaaatt gtatccaatg gatcgaacat ccttcgatcg aagcgacttc
     2641 tatgctaatg aaccatccag gtatcgcgat cgtcttagcg acaggtggag ctggaatggt
     2701 caagtctgct tactctacag gaaaaccagc tttaggtgta ggtccaggta acgtaccagc
     2761 ttatattgaa aaaacagcaa aaattaaacg tgcagtgaac gatttgatcg tttcgaaaac
     2821 atttgataat ggaatgattt gtgcttctga acaagctgtg atcgtggata aagaaatcta
     2881 tgcagcagta aaagcagaat tccaagcaca ccaagtttat atcgtaaaac cagatgaatt
     2941 gcaaaaatta gaagatgcag tgatgaacga aggaaaatat gcggtcaatc catcaatcgt
     3001 tggacattct gcgatggaaa tcgcaaaatt ggcaggaatc agtgtaccaa aaggaacgaa
     3061 aatgctgatt gctgaattgg aaggcgtagg cccagattat ccgctatcaa gagaaaaact
     3121 ttctccagtt cttgccatga tcaaagctaa caacacggac catgcgtttg atttgtgtga
     3181 aggtatgttg aatcttggag gattaggaca tacagcagtt atccattcag aaaacgaaga
     3241 gttgcatgtc aaattcggtc ttcgaatgaa agcttgtcgt attttagtga atacaccatc
     3301 tgcagaaggc ggcatcggtg atatctataa cgaaatgatt ccgtcattga ctttgggatg
     3361 cggttcttat ggtaagaact ctgtttctcg taacgtctct gctgtaaact taatcaatgt
     3421 caaaactgta gcgaaacgga gaaataacat gcaatggttc aaattaccgc caaaaatctt
     3481 ctttgaaaag aactctctat tgtacttaga gaaaatggaa aacgttgaac gtgtcatgat
     3541 cgtttgtgac cctggtatgg tccaattcgg ttactgcgat acagttcgtg aagtattggc
     3601 acgacgtaaa aatgatgtga agatcgaagt cttctcagat gttgaaccga acccatcgac
     3661 aaataccgta tacgcaggaa cgaaactgat ggcagacttc aagccagata cagtaatcgc
     3721 tcttggaggc ggttctgcaa tggacgcagc taagggaatg tggatgttct atgaacaccc
     3781 agacacatct ttctttggtg caaaacaaaa attcttggat atccgtaaac ggacatataa
     3841 gatcgacaaa ccagaaaaaa cacaattcgt atgtatcccg acaacatcag ggactggttc
     3901 tgaagtaacg ccatttgcgg tcatcactga cagtgaaaca catgtgaaat atccgttagc
     3961 agattatgca ttgacgccag atgtcgcaat cgtcgatcct caattcgtca tgtcagttcc
     4021 agcatctgtc acagcagata ccgggatgga tgtgttaaca catgcaatcg aatcctatgt
     4081 ttcagtcatg gcttcagatt atacacgtgg attgagccta caagcaatca agttagtctt
     4141 tgactattta gaaaaatcag taaaaacacc tgatatggaa tcacgtgaaa aaatgcacaa
     4201 cgcttctaca atggctggta tggcgtttgc taacgcattc ctaggtattt gtcactcagt
     4261 cgcacataaa atcggcgggg aatacgggat ccctcatgga cggacaaatg cgattctttt
     4321 accgcatatt atccgctata atgcaaaaga tccacaaaaa catgcgatgt tccctaaata
     4381 tgactatttc cgtgcagata cagattacgc agatatcgca aaattcttag gtctgaaagg
     4441 cgaaacaaca gaggaattag tcgaagcttt agcaacggct gtttatgaat taggaaaatc
     4501 tgtcggcatc aacatgagtc taaaagctca aggagtcaca caagaaacac tagatacaac
     4561 cgtagaccgt atggctgaat tagcttacga agaccaatgt acgacagcta atccgaaaga
     4621 accattgatc tctgaattga aacagatcat cattgatgct tacaacggat aagcaaaaga
     4681 aagcgtagaa aaaagataag aaaatagatg aaaaagaact tggaaaacgt tgtttctcca
     4741 agttcttttt ttaatttcca agtaactgaa agttactttt gttatttaac atttaggtca
     4801 attttagaca gtttttgttc aaaaagcgat atttatagtg gacggtatta ctgctatatt
     4861 aaggatataa aatgaaagcg tttaaaaagg tggcgtaaag atgattatta aagaagagat
     4921 ttttgtacct aaagaattag tgaaagaaaa aattgacctg ctcgttgaaa acctgacaac
     4981 gatcaaagac gataatggtg aatttttgtt ggactttgat ggactgaaag tagatgacaa
     5041 aagctggaca gtgtggaatt ggccacaagg tgtcggtcta tatggtattt ataaaaatta
     5101 ccagatgaca aaaaatcctc gcgcttatca agtggtcaat gaatggtttg aggatcgtat
     5161 ggaagaaggc gctccgccca aaaacgtcaa tacaatggca ccgctattga ctatggctta
     5221 cttgtatgaa gacacgaaag attcacgcta tttgccttat ttagaacaat gggcagaatg
     5281 ggtcatgaat gacatgcctc ggacaaatga agacgggtta cagcatgcca catatggacc
     5341 ggaaaataag aatcagttgt gggacgatac attgatgatg acagtgcttc ctttggcaaa
     5401 aatcgggaaa ctgttgaatc gcccagaata tttagaagaa gctcgttacc agtttatgat
     5461 ccatatcaaa tacttgatgg ataaacgaac aggattgtgg tatcacggct ggacgtttga
     5521 aggaaatcat aactatgccg aagcattttg ggctcgtgga aactgctggt tgacgattgc
     5581 tattccagag atcattgaaa tcttagaatt atcgaaagaa gatgcattgc gcaaattatt
     5641 gatcgaaaca cttgaagcac aagtgcgtgc attaaaaact tatcagtctg aaagcggttt
     5701 atggcatacg ttgttggatg atccttcttc ttacgtagaa tcttctgcaa ctgcaggatt
     5761 tgcttacggt attttgaaag cggtccataa acgttattta ccacaagaat ataaagaagt
     5821 cgcttatcgt gctatccaag gattgcttga acaaatcgat gaaaaaggag aagttcaaaa
     5881 cgtttcaatc gggacaggaa tgggggacag tttagatttt tatcggaata tcggaatcac
     5941 agcaatgcct tatggacagt cgttaacggt gctttgtttg acagaattgc tcgtctctta
     6001 ttgttaaaag aaaggaaacc catatgaaaa aaacatcact gctgtttgca gcaagttcgc
     6061 ttagcctagg tctgctaggt ctaagcggct gcggaaataa ttctagtgtg gcaactgtcg
     6121 cagatgcaga tgtaaatgag atagttacct tgcgctttgc ttatgcaagc aacagccagc
     6181 cagtaattga tgcaatgaat gaatttgggc gtttagtaaa agaaaaaaca gatggttccg
     6241 tacagatcca gtatttccca gatggacaac tgggtggaga acgtgaactg atcgaattga
     6301 cacagtcagg agcagtagat ttcacaaaag taagtgcctc agcgttggaa agtttctcta
     6361 aagattattc gattttttca gttccttatc tattcaatga tgaagaacat ttctttaagg
     6421 tgatggataa tgaagaaata atggagccaa tctaccaatc gaccaaaaat ttaggattta
     6481 ccggattgac ctattatgat tctggtcagc gcagctttta tatggtagac ggtccgatcc
     6541 atacgccgga agatttgaag ggaaagaaga tccgtgtgat gcaaagcgaa accgctatca
     6601 aaatggtaga gcttttaggc ggttcgcccg tacctatggg aagtgatgaa gtgtacacgt
     6661 ctcttcaatc caatttgatc aacggatcag aaaacaatga atttgtcttg tatacagctg
     6721 gacatggtgg tgtggctaaa tactattcct atgatgagca tacacgtgtg ccagatatca
     6781 tcatcatgaa tgatgccatc aaagaacagc taactgatga gcaggaaaaa gccattgaag
     6841 aagcagcgaa agaatcaacg gtttttgaaa ttgaagcttt tgcaaaagca attgaagaag
     6901 aaaaagcttt agcacaagaa gaacatggag tccaattcaa tgatgtagat aatacaccgt
     6961 ttcgagaggc agttgcccca cttcatgata tattcaaaaa cgatcctacc tacaaagacc
     7021 tttatcaaaa gatccaagag caaggagcgt agctgtgatg aaaatgattt taaacaaggt
     7081 attagagatc ctgggagctg ttttgcttgt tgggatggtc gctgttgttt tatggcaagt
     7141 attttccaga acggttttag gaaatccaaa tacggtcacg gaagagcttg ttcgattcgg
     7201 tcttgtctgg tttgccatgt tgtcttccgc gtatgttgtg ggacaaaaag cacatttagc
     7261 cgtaacggtt ttaagtgaaa aattaactgg caaaaaaagt aaaattttag aagttgttgt
     7321 tcaaagttta tttctggtat ttgctgcgat gatcatggtg tatggcggat ggaatgctgt
     7381 cacattgaca atggggcaga tctcgccttc tttaagtatt cctatgggat atgtttatct
     7441 gtcagtacct gtatcggggg tactcatcat tatctatagc ctgatcaatc taatcgatac
     7501 attaagcaac aagccagcaa ttcaagaaaa aatataaaaa tggaggaata aaatggcaat
     7561 agaagccgga ttagtgttat tttttgtatt tgttgtgttg cttgtattag ggatgccgat
     7621 tgctataagt attgcattct catcgatgat gactttattg ttagttatac cgtttgatgt
     7681 ttctgctttt agttcagctc aaaaaatggt gggaagtatc aatagttttt cgctgatcgc
     7741 tattccgttt ttcgttctct cgggcattat catgaataat ggagggatag ctaagaaact
     7801 agtggatttt gcgatgttgt tcgtcgggcg cgtgccgggg gctttggctc atacgaatgt
     7861 gttagggaat gctttatttg gttccatctc tagctcggcc attgccgctt ctaccgcgat
     7921 tggcggagtg ttgatccctc agcaagtgga agaaggatat gaccggaagt ttgcgacagc
     7981 ggtcaatatc gcgtcagctc cgacaggtat ggtcattcct ccaagtactg cttttatcat
     8041 gtattcactt gttgctggtg gtgcttcgat ctctgctttg tttatgggag gttatctcgt
     8101 aggcgctttg tgggcgctag gaatcatgct agtagcattc atcgttgcta aaaagaataa
     8161 ataccctgtt gtcgaaaaag gacagatggc tcatgctgga aaaataataa aagaggcgat
     8221 tcctagcctt ctattgattg tcatcattat cggtggtatc ttgacgggga tatttacagc
     8281 agtagaagct tcagcgatag ctgtcattta ctccctgttg attgctatgt tttactataa
     8341 aacggtaaaa ctaaaagata tacctaatat gttgtttcaa tcagtagcaa tgtcagggac
     8401 gatcatgttc ttattggcta cttcttccat gatgtctttc gccatggcgt ttaccggtat
     8461 tcctcaggca attagttcgt tgattatggg cgtaacggat aataaattta ttattctgtt
     8521 attagtcaat cttgtgttat tactggtggg tatgtttatg gatgtggcgc ctgctatctt
     8581 gatttttaca ccaatatttt taccaatcgt cacaagtgta ggggttgatc ctgtacatta
     8641 cgggttgttt tcaatcatga atctgtgtgt aggctccatc acgccgccag taggtacagg
     8701 actatatgtc ggtgcaagtg taggaggcgt aaaagcagag gcaatgctga aaccgcttct
     8761 tcctttttat ggggttatct tagtcgtctt gtttttgatt acttacttcc cgcaaatcgt
     8821 catgtggcta cccaatatgc tgagttagga gaatagttat gttgaacatt acaattagag
     8881 gacatgatct ttccgaagtc caaacgatag aagatcttgc agaaaaaaca aaagaacaag
     8941 ggattcatac actgcagctc gccttaggtc tttcgtttcc tgaaatacct tctggcgcaa
     9001 atgagatcaa ttctgggatg ggaaattatg tcaaacgagt tttagaaaag caagaagtga
     9061 ccgtaggaat tttaagttgc tatatcaata tgattcatcc agatttaacg atacgacaag
     9121 agttgctgac aaagttcgag cagtatcttc gctatgcttc ttcctttggt gcgtcgatgg
     9181 ttgcttcgga aactggatgt gttttgccgg aaatccaata tacggaagaa aatttcacag
     9241 atgaagcatt tgctgaagct gtgtccgtta tccgtcgctt ggtaaaagct ggagaaaaat
     9301 accaaatgat ggtggggata gaaccaggtt tgaatcatcc ggtgtattct ttagcaaggg
     9361 tagagcagct gatccaagca gtagattcgg actatctagg aatcatttta gatccgacca
     9421 atttgatcac aagtagtgat tatcaagaac aagtccaatt ggtagaagaa gcttttgaac
     9481 gttttggtga aaagatatgt gcggttcatt tgaaagattt tcgtgtcgaa caggaaaaaa
     9541 tcgtgccggt aaatttggga gacggtgtga tcgagtacac aaaaatcaaa gaaatcatca
     9601 tgaagaatcg tccgtacctg tatgtggttc ttgaagaaac aaaagatgat gggattcgtt
     9661 atggaagaag tcttcttgaa taatgtaaag aagacgttgg ttaactaata tttagaatgg
     9721 agggggcggg gctagaagat gccacgtctc ttctttttta taagagtata taaaagcagc
     9781 tctttttgat tacaaaaaac gtggtataat aaaattgtgc ataagtgaac aaaaggaggt
     9841 gcggcacatg ctccaaaaga aaatcagcgg tgaacaagtc aatacgatgg tttctgatca
     9901 gtatgaaatc tatcatgtca aagatgtcgt cagccaaaat aaaacgatct atcatcatca
     9961 tgatttctat gagatccatg ctacattaaa gggagaagcg actttttatt tgaacggacg
    10021 acaatttacg atcaaagcag gaagtgtgct gctcatccat tccaatgatt tgcatcgtat
    10081 cgtccgccaa tcttcagatg tattcgagcg tgtctatatc tttgtcaccc cttcttttct
    10141 agaaagtcgt tccacgccat ggtctaatct atcggcttgt tttcaaccaa tgggagagaa
    10201 gaggagtcgt gtattgcaaa tagaacagga agtattgaag caaaaattat catttatcga
    10261 ccatcctcct gataaaaaaa tttacggtga agacataaaa tatgaacaag ctctggtaga
    10321 atacttgatt tttctcaatc aagttgtaca aaaagaagag agtgcgcaag aacagaatgc
    10381 tgtagttcct aatgagcgtg tggaacaaat gttacaatat atctcaaaaa atttatctca
    10441 tccgctttct ttagaggaaa tggaaaagca gttttttgtc agcaaatacc atatcacacg
    10501 agaatttaaa aagtacactg gatttacgtt tcatcagtat gtaatgaaga aaaagctgat
    10561 ctatgccaag cacttgttgc gagaataccg cagctctagt gctgtatata gtaaaagtgg
    10621 tttttcttct tatccgcatt ttctgaaagc attcaagaga gagttcggca taacgcctaa
    10681 agaatttttg aaacgtgata cacagaaaca gttggttcat tatgaacatt ttgaagatga
    10741 acaatgagaa tgatcactaa aaaataaccg cagtagctgc tgttgtttac atagaagcag
    10801 aaactgcggt tattttattt ttatctatat atttcaaaat attttgaaaa aatgacgtac
    10861 atattatatg aatcagctga atttacgaaa gtctctcggc gatagtcctg tttttgcttt
    10921 gaacaggcgg ctaaagtaag caaaattctc aatccctatt tcttcggaaa tcattcgaac
    10981 cgtaaattct gtataccgaa gaaggtcttt agcgtaagaa agttttttgc tgatcaaata
    11041 atcgattggt ggagaaccga taaatttgga aaaatctttg ctcagctgat atttattcaa
    11101 taagaattgt tgttctaatt gatcaagtgt aatcgtttct ttgaaatgtt cgtctaagta
    11161 ttgcttcagt ttgtaaacgt aggcgggaat atcctcttct tcgaaatcta gctgatactt
    11221 ttgcaaaatc agttcattca gcaactcgtg gatagctaca gaggactgga aatcggtacg
    11281 ggcattcgtg ccttgttgca tgtggatcaa ttctgtgatc aaatggtgga tcttgttttc
    11341 tgatggatct cctgttgaat ggaaggtgtt atggccgtct tttgtgaact cctgataaaa
    11401 tgattggacg ttctgaccat tcaaatggat ccaatccatt tcccatggtt cttcgctgat
    11461 cgtttgataa cgctgatatt cttggcagtc aataaaaaat aagtctccag cttttagctg
    11521 ttgctgatta tcattatatg taagcgaacc tttgccagac aaagtaaact ttatcaagta
    11581 agaaggtaaa tgtgctcgtt cagtatagta agggattggt gctttgaagt aaccgatttc
    11641 ttggatataa aaaaataaat tcctgcttct ttgtgtaggc gtcaagatca ctctttttga
    11701 gtcttccgac catgagtggt tggctttttc ccagtaatcc atgtaaacac tcccaacttg
    11761 tcaagataat gcaattatat accaaaatag tgtgaagcaa aatataagat tctgtattat
    11821 gatagcattg tcaataaatg ataacgcttt attaataaag tgctaatcaa caataaagta
    11881 aaagaggtgg agaaatgaat ccaacatttg ttgccatcga tatcggtgca tcaagcggac
    11941 gactaatgaa aagtacgagg gatgcaaaag gttctttgat cttggaagaa gtacaccggt
    12001 tcaaaaacgg ctttcggaac aaagacggtc acgatcgctg gaagatcgat tatcttatca
    12061 aagagatttt gattggattt gaaaaattaa agcagacagg gattgatcgc ctttttgcgg
    12121 ggatcgatac atgggcagtc gattactgtc tgattgatga acaaggggat cggataaatg
    12181 atccgattgc ttatcgggac aagcgtacca atggagcagt agaacagttt tctgaaatct
    12241 attctctaga taagttgtat gagcagacag ggattcagat ccagcctttc aataccttgt
    12301 tccaattgtt tgctgaagaa aaagaaaatt tggcaaaagc acataaattg ttactgatac
    12361 ctgattattt agggtatgtc tttactggta acatggtgac ggagaaaact aatgcatcta
    12421 ctatgcagct gctgaatgca gggacaaaag aatgggaaat agaacttctt gaaaaaatcg
    12481 gtgtctcgaa ggaattattt ccaccattga cagagccggg aacgatacta ggagatctgc
    12541 aaaaagaaca attttcaaaa tatgatttgc cagatgtgac ctttatcaca gtagcaagtc
    12601 atgatacggc gtcagctgta ttaggaacgc caggtcaagg agaacgttgg ggttatatca
    12661 gcagtgggac atggtcgctt ttagggattg aaacaaagat cgccaatgtt tctcgcaatg
    12721 ctttttctga aaattatacg aatgaatggg gtgcccataa cacggtgcgt tttttgaaaa
    12781 acatcatggg gatgtggttg atccaggaag ttgcccgaat gcaagagtac gagtacagtt
    12841 atgcggaatt ggcagagctt gctgcaaaag aaaaaccgct gcagcaagtg atggaaatca
    12901 atgaccctcg atttttgaat cccccaaata tgatagaaga gattcagcaa tactgttttg
    12961 aaacagagca gccagtccct gtctcatcgg gagctatcgc tcgttgtgtg tatgataatc
    13021 ttgctctttg ttacgcacaa gagctgatca aagtagaaca cttaactgga acgaaattgg
    13081 aaaagctgca catcgtaggg ggcggatcga ataatcgtct actgaatcaa ttgacagcag
    13141 attatgccgg aatcattgta gaagcaggac ctagtgaagc cacagcaatc gggaatgttg
    13201 ccatgcaaat gctcagtaca ggtcagtttt cgaatataga acaagcaaga aatgtaatca
    13261 aacagtcgtt tccatgtgaa actttttacc cagatgaaaa taagcaaaac acgatggaat
    13321 ggtacgtgaa taaaatcaaa ggagtaatcg tatgaacacg attgaaagaa attacgaaca
    13381 agcaaaagaa aaatatgcaa ctatcggtgt agatacagat gctgtactag aaaagataca
    13441 ggacatcaaa atttctatgc attgctggca aggagatgat gtcaaaggtt tcttgacacc
    13501 ggatggcgaa ttgaccggtg gaatcatggc gacagggaat tttccaggag ccgcacgaac
    13561 acctgaagag cttagacaag atctggagaa ggcttattcg ttgataccgg gcaaacacaa
    13621 attgaacctt catgctatct atttggatac agaagaatct gtcgatctaa atgaaattga
    13681 gccgaaacat tttgaaaaat gggtagagtg ggcaaaaaaa gaagaaatcg gtcttgactt
    13741 caatccgact ttcttttcgc atccaatgat gaaagacggc tttacattgg ctcatccaga
    13801 taaagaagtt cgtgaatact ggatcgaaca cgggaaaaga agccgcaaaa tcgcggagta
    13861 ctttgggaaa gaaactgggc aaacaagtat caataacttt tgggtacctg atgggatgaa
    13921 agataatccg attgatcgtt ttaccccacg ggaacgttta atggaatcac tcgatgaaat
    13981 ttttacagaa gagttagatg aacggtatgt acaagatgca gtagaaagca aactatttgg
    14041 tttaggcgct gaagcttata cagtcggttc ccatgaattt tatatgggtt acgggttgac
    14101 tcgtgacaaa cttgtttgtc tagatgctgg acattttcat ccaacagaag tgatttccaa
    14161 taaattatct tcattagctt tattcagcaa aggaatcatg ttgcatgttt cccgtccagt
    14221 gcgttgggat tcagaccatg tggttttgat ggatgatgaa ttgcaggaaa tcgctaaaga
    14281 attagtccgt aacgaattgt tggaaaaaac aaatatcggt ctggatttct ttgatgcaac
    14341 gatcaaccgt atcgctgctt gggtcatcgg tacgagaaat acacaaaaag cactgctgaa
    14401 agctatgctg gaaccagttg agcgattgaa agaaatggag ctggcttttg actttacttc
    14461 aagaatggct tatacggaag aattgaaaga tttcccgtat gccgatgtat ggaattattt
    14521 ctgttacaaa aatcaagttc cagttggttt ggattggctg gaagaagtcc aggaatatca
    14581 aaaagacgtg ttggaattaa gaaaatagga gtgtggctat attgaaaaaa atagatattt
    14641 tacaagcgcc ttatgtgaaa gaaatgatcg aaacgacgtc gaatctttat cgcttgggat
    14701 gggatgaaag aaatggcggg aatatttctt atttactaaa agaagaagag atcacaccgt
    14761 tcttggatcc tgaaaaagtg atccgccgca tctcgatgat ttttgatgcc agccgtttag
    14821 caggatgcta ttttattgta actggttcag gaagatattt taaaaatgta gcgagcgaac
    14881 ctgccgagaa tcttggaatc gttcgagttg ctgaagatgg caaaacactg gaacttttgt
    14941 gggggctgga aaacgaatgc cttcctacaa gcgagttgcc aagtcatttc atgagtcata
    15001 tcgcccgttt agaagtagac ccagataatc ggatcgttat gcattgccat gctagtcatc
    15061 tattagcgat gagttttacg catgaattag acgaacgttc ctttagtcgt acgctatggc
    15121 aaatgtgtac ggaatgctta gttgtttttc cagaaggaat ttccatcatt ccgtggatgg
    15181 ttccgggaac aaatgagatt ggggaagcaa cagctgaaaa aatgaaagaa actcgattgg
    15241 ttttatggcc acaacatgga atctacgggt caggaaaaga catggatgaa gtctttggtt
    15301 tgatcgagac agcagaaaag gcagcagaag tctatacgta cgtcaaagcg caaggaccaa
    15361 tcttgcaaac aatcacggac gaaaacctgt ggcatttagc agatgcgttt ggtgtcactc
    15421 caaaagcagg ttatttggag gaagttcata cgaaagcagg tgtctgacat ggtgaaaaaa
    15481 gctttttgca tgaaagttta cccagaaaaa caagaagaat acaaaaaaag acatgatgaa
    15541 ctttggccag aaatgcgtac agcacttcgt gaacacgggg tcatcgacta ttcgatctat
    15601 cttgatccga gtacaagttt gttatttggt tacttagtga tcgaagatga aaagttatgg
    15661 gaaaaaatgg caaatacatc tatcaatcaa aaatggtgga agtacatgga agatatcatg
    15721 gagacaaatc cagatgcctc tccagtcagt tgggaattga tgccagtctt tgaactgtag
    15781 caacaagcaa ctgggcaacc ggttgcttgg ggttctttcg tttttattgt gaaaaataat
    15841 acaaattagg aggaatagaa aatgagtaat cgaatgattt taaatgaaac atcgtatttt
    15901 ggaaaaggag cgattgagtc gatcgtccct gaatttcagc agcgaggatt cggcaaagca
    15961 gctgtcatta ctgacaaagg attgatcgag cacggtatag caacaaaagt aacagaactt
    16021 ctagataaag cagcaatcgc ttatgcttta tacgacggga tcgtacctaa tccaacgatc
    16081 caaaatgtaa aagatggagt ggcttttgtt aaagaggcag aggcagactg tttgatcgca
    16141 atcggtggag gttcgccaat cgatacggca aaagccattg ggattatttt gacgaatcca
    16201 gagttttcag acgtcattag tttagaaggt gttgctgata cgaaaaatcc atgtttacct
    16261 atattagcta taccaacgac ttctggaaca gcagcagaag taacgatcaa ctacgtcatc
    16321 acagatgaag taaatcaccg aaaatttgtt tgtgttgatc ctcatgatat cccaatcgtc
    16381 gcgttcatcg acagcgatat gatgatggga atgccgaaaa aattggctgc atcaacaggt
    16441 atggatgcta tgacgcatgc tattgaaggg tttatcacaa aaggcgcttg ggaaatgact
    16501 gatatgctgc atttaaaagc aatcgaaatc atcggccatt ctttggaagc ttctgtagat
    16561 ggcgatcaaa atgggcgaga aaaaatggca ttaggacagt atattgcggg aatgggcttc
    16621 tcaaatgtcg gtttaggact ggtacacggg atggctcacc cgttaagtgc atggtacaac
    16681 attcctcatg gcgtagcgtg tgcagcactt ttgccaactg tcatgaaata taacaaagaa
    16741 tatactggag aaaagtaccg tgagatcgct cttgttctag ggattaaagg agcagctgaa
    16801 atgtctttag aagatgtaag agaagcggct tgtggtgaga tcgatcggtt gagcaaagcg
    16861 gttggaatcc cagaaaccat ttctgaatta ggtgtaaaag aagcagatat tccagcaatt
    16921 gcagaagatg ctttgcgaga tgtatgcaca ccaggaaatc caagagaaac gacagtagaa
    16981 gaaatcattg ctttatatca aagtttgatg taaaaacgaa gcggatcttc aacattaaat
    17041 tgcctgtaaa gagaaatggt agtttatatc tggatatctc ttttaaattg atggtatttc
    17101 cgctataatt tttcttataa agaaatagga gaatattata tggaaaataa aaagccttat
    17161 ggaactgtat tgatcaaagc ctctaaaata ctagactatt tagcggaaaa ccaagatgtc
    17221 tctttgcaga cgatcgcaaa gggtgtggga atgacctctt ctacagtctt gaaaatacta
    17281 gatactttgt tgatgatcgg ctacgtaaat aaaaattcag agaaaaacta tcgtttaggt
    17341 gcaaagctta ttcgctacgc gaataaaaac attgaacaaa tcgatttggt agagttgact
    17401 ttaccgtttt tagaacagct gcagcaaaag atcgatgaga ctatccactt aggggtattg
    17461 gacaacaatg agatcttgta cgtgaacaaa ctagatccaa aacatcaaac gatccgcatg
    17521 tcttcaaaaa tcggaatcac ccgaccatta tacaattctg cgatgggcaa agccgtatta
    17581 gcagaattca gtgaagagca gtacactgat tatcttgaaa agcatccgtt gatcccttac
    17641 acagaatata cgatcaccaa tgcattacgg ttgaaaaagg aaatcaaaac ggtaaaagaa
    17701 acccacatcg catttgacga tgaagaaatc gaacgtgata ttttttgtat aggagcctca
    17761 atcgtgaaaa acgaacaaat cattggtgca tttagtgtaa gtatgccgaa atatcgttta
    17821 acagatgaga caaaagaaca aataatcagt gccctcaagc aaacaaaaag cgaaatcgag
    17881 caaagaattc aaaaataatg aaaaaagcgt ttgcaatttt tcttttctgc cgtataatat
    17941 gggtatagat ttccaagtta taaaataaaa tttctaaata ggaaataggt ggagaagatg
    18001 aaaaaaattg atattttatc tcgattgaaa aacgccggag tgattgcggt ggtacgcgga
    18061 aaaagcaaag aagaagcttt gaatgcttgc cacgcaatca tcaaaggtgg tctaactggt
    18121 attgagttga cttttacagt ccctcaagca gatcaagtaa tcaaagaact tttgtcattc
    18181 tacaaagacc aaccagaaat agtgattggc gcaggcacag tattagacgc tgtcacagct
    18241 cgcttggcta tcttagctgg cgcagagtat attgttagcc cttcctttga tcaagaaacg
    18301 gcagagatgt gcaatctgta tcaaattcct tacttaccag gatgtatgac tattaccgag
    18361 atcaaaacag cgctgaaaag cggggtagat atcgtcaaac tatttcctgg aagcgcttat
    18421 ggaccaagta tcatttctgc cttcaaggcg ccgatgcctc aagtcaatat catgccaact
    18481 ggcggtgtga gtctggacaa tatgaaagag tggttcgatg ctggtgtcgt aacagtcgga
    18541 gtaggtggga accttttagc acccgcagct acaggagatt ttgacaaagt aacggaagtc
    18601 gcgcaacagt atgcagcaaa aatgaaagaa atcaagaggt agctgcttat gggaaaagta
    18661 gtgacactcg gagaaatcat gttgcgattg tccacagagc agggaacccg tatcgattca
    18721 agtgaacgat tcgctgccca ttatggcgga ggggaagcaa acgtagcaat ttctttagct
    18781 aattacggac atgaagcgat ttttgctaca aaagtacctg ataacgcatt gggaaaagca
    18841 gtcaaaaagc acttgcaatg ttatggagta gatacgaaac aggtattatc tggtggatca
    18901 agactaggta cttattatat ggaaactggt agtggcgaac gcgccgcctc agtgatctat
    18961 gatcgtgccg gatcaagttt tgctgaaatg gatcatttgg aatggtcgct agaggagtta
    19021 ttccaaggaa tagatatatt tcatgtttca ggaatcaccc cagctttgtc taaaaaatgg
    19081 caaaaaatga cgaaacaggt gatagaagct gcaaaaaaag ctggctgcct gatcagcttc
    19141 gatatcaact atcgtggaaa attatggaca cagcaagaag ccagcgaagt aatccatcag
    19201 cttttacctt tagtagatat ctgctcagct ggaaaaatgg atgctcttta tttgttaggt
    19261 atcgagaaag cgccagaagc agaagaacaa cctctaattt attattatca aaaaatgcag
    19321 gaaaagttcc cgaatattca ggtgttttat tcgaccaagc gtaccgttca ttcagcaagt
    19381 gaaaatgaat tgaaggggac attgtggatg aaccatgaat atgtggaatc ccaagtccat
    19441 agaatcaccc caatcgtgga tcgagtaggt ggcggagatg cttttgcagg tggcgtgctc
    19501 catgggattt tatccaatat gtctccgcaa gaaatcattg attttgctac ggcagcttct
    19561 gccttgaagc atacgatcca tggggactgc aaccaattta gtcaagaaga agtcgcacaa
    19621 tttttagcat gcggctctgg aaaaatcatt cgatagaagg agtagaagaa aatgcaagaa
    19681 atggaaacac gttatacaca cagtccagaa gatatccgtc attattcaac ggaacaattg
    19741 agaaaagaat ttttagtaga aaaggtattt gaaccaggga aaatcagttt gacttatacg
    19801 cacaacgatc gaatgatctt tggtggagtg acacctactg aaaaagaatt agaaatcaca
    19861 ttagacaaag aactaggtgt tgattacttc ttagagcgtc gtgaactagg tgtgatcaat
    19921 atcggtggac caggattcat cgagatcgat gggcaaaaag aagaaatgaa aaaacaagac
    19981 ggatactata tcggaaaaga aacacgacat gttgtatttt cttctcaaga tgctgaaaac
    20041 ccagctaaat tctatattag ttctgttcca gctcatcata aatatccaaa tgtaaaaatc
    20101 agtatcgacc aagtaaaacc aatggagact ggtgaaggcc tgacactgaa tgaacgtaaa
    20161 atctaccaat acatccatcc aaatgtatgc gaaagctgtc aattacaaat gggttatacg
    20221 attttagaac caggaagcgc ttggaatacg atgccttgtc atacacatga acgtcgtatg
    20281 gaagcttatg tctactttga ttatgccaat gaagatacac gtgtgttcca catgatgggc
    20341 aaaccagatg aaacaaaaca tttagttgta gacaatgaac aagccattat ttcgccaagc
    20401 tggtcgatcc attcaggtgt aggtacaagc aattactcat tcatctgggc aatgtgcgga
    20461 gaaaatatca cctatacaga catggatatg gtgcaaatgg atcaattaaa ataagcataa
    20521 gggagagaaa atgatggaat ttagtatgga tatgtttcgt ttagatggaa aagtagcatt
    20581 agtcaccggt gctgtctatg gtattggatt tgagattgca cgctctttag ctacagcagg
    20641 agcaaaaatc gtatttaata acttgaatca agaatcagtt gatgaaggta tagcgcatta
    20701 taaagaagca ggcatcgatg caaaaggtta tgtctgcgat gtgaccgatg aagaagcggt
    20761 tcaagcgatg gttcaacaga tcaaagaaga tgtaggttca gttgatattc tagtcaacaa
    20821 tgcagggatc atcaaacgga caccgatgat cgaaatggat gcggcagact tccgccaagt
    20881 aatcgacgtg gatttaaatg ccccattcat cgtttcaaaa gcagttattc cggatatgat
    20941 cgaaaaaggc ggcggtaaaa tcatcaatat ctgctccatg atgagtgaat taggacgcga
    21001 aacggtcagt gcttatgctg cggcaaaagg cggattaaaa atgctgacga aaaacatcgc
    21061 ttctgaatac ggtcaataca atatccaatg taatggaatt ggaccaggtt atattgctac
    21121 accgcaaact gctccattac gcgaaacaca agaaaacggt gaacgtcatc cattcgatca
    21181 atttatcgtt ggacgtacgc cagcagcacg ctggggcgag ccaatcgatt tggctggacc
    21241 atctgtcttc ttggcttcta gcgcatctga tttcgttaac ggccatgtct tgtatgtgga
    21301 cggaggcata cttgcctata tcggaaaaca accttaaaag agctttagta cactgtttag
    21361 tggagaggtt gtgaaatcag cggattgaac gaataccgaa ggacagctga ttaaacatca
    21421 tttattcgaa aaaagaggct gggacagaag catttaactc caagaaataa gaaggaattc
    21481 acgaaaattg cttttcaaat ttttgtgaat ttcagcttat ttccgaagga gttgctttta
    21541 cttccgccgt ttgtacgtgt ttagagcgtg agacaaaagt gttctttact tttgtcccac
    21601 gctcttttct gtgcgtcttc catacttcac taagtcgatc cctcttgctt cgcaccaatc
    21661 cttcactggt ccggaaaaga tcagagattg ttggtgtagg gccgcggtat tcgttgcgcc
    21721 gaccatagta taaagcagac gaagctcttc ttgccattgc ttcatcagta taatagtttc
    21781 ttctacgccg tgattcatca gatgcgtcag tacggttcct gaagtaccca cggcatttgc
    21841 gccgagacaa agggctttaa atatgtcata ggcattgcgg atacctccgg aggctaggat
    21901 ttccatagaa gtgtctgctt catttgcttc aagcaatgaa gagactgtcg attgccccca
    21961 gtcagctaaa taactcagtt cccgcttgga gcgacgggcg ttttcgatct gggtgaagct
    22021 tgttccgctt cgaccgctga tatcaactgt atgaactcct aaagcagcta gatcattgag
    22081 cgtttctctt gtcatgccaa aaccgacttc ttttacgatg agggggacgt caatggctgt
    22141 ttgagtttct tgaataagta ctttccaatt ggtaaaatca cggtctccct caggcataac
    22201 caactcttga ggtgcattca aatgaatctg taaggcatct gcgtgaaaca atcggatagc
    22261 ttctttggct ctttcgacag aagtccctgc accgatgttg gcaatgattt tcccgtgagg
    22321 atattcttgt cgcataatcg tataagtatc ggctaaagaa gggactttca aggcagcgct
    22381 gactgaacca gtggcaatca tcaaatctgc ttctcttgcg acgattgcaa gatcttggtt
    22441 gatttttttt gttttttcgc ttccgccagt catcgcattg atataaaatg ggctgcttag
    22501 tgtaaaccca actgcctgtg tacttagatc gacatcagaa actgctattt gcggcaaagg
    22561 gttatggatg atccgtacga agtcaaattc gtttttttgt ttatcatgaa aggctttagc
    22621 tagtgaaaca tgttcgtctt ttcgattcat cggttttcct ttctttgatc gctatagaca
    22681 tgtaacggca gtggagtgat ttctgctttt tcccatgcac tcattaaagg aagaatgcca
    22741 gatttctggt caacaattac gattccgcaa tcgcccccac ctgcaccaga agattttgcg
    22801 gctccttcat actgttcagc taaattacac aatttgttca aagcaggcgt ttcgatgacg
    22861 accccagtga ttgcagataa atcatgcagt aattgtcggt tttttcgaat catctgttgg
    22921 atcaacgtta cattattttc tttaaaccct ttgatcattt cgttgacaca ttctgtacta
    22981 ttttttaaga aaagctgata agccaccatt ttatcttctc tcgaacggtg aacttgatcg
    23041 accaaatcag aagtagaggc agggctaccc gtccaaccaa tcaataaacg taaatcttca
    23101 ggagcaatca atggctcaat ggatagacct ggccaatcca gggcaagtaa ctcactgata
    23161 gaatgctgag tttcttgttc ttggagccaa ggatggtcga aggttgagaa agcgatccag
    23221 ccaccataac agctagcagc gatgtcgccg caggaaccat tatcttgaac tgctaaattg
    23281 gctagtgctg caatcttgaa aatctccaac tgagataaat tcaacgcgta aaaaacattc
    23341 aaggctttga ccgttgcaac ggtcacggca ccgctagaac ccaatccata tttccggcca
    23401 tttgaactgt ctaactcact tgttactttc agatcataaa aggataaaag aatgtttttt
    23461 tcttgtgcat acttttcagt caagcgaata gcagcaagga tataatgaaa aggattttcc
    23521 cgaatatcta aaactaattc tccgttgcgt cttgtccaac gtacaggcat cccactatat
    23581 tgtgcagatt ggatacttcc gacttttcgt gcagattcta cagtgactgt cacgaattga
    23641 tcgactgcag cgataactgc tggatggcct gtttcaacaa ctgcatattc tccggcaata
    23701 tagagtttgc ctggtgcaga tacttcaatc ataaaaatct ccctttattc aataatcgca
    23761 attcctggac cagcatgtgc agtaattact tgttctttgc taaataattt ggtaaatgtt
    23821 tcttgtacct cagataaatg gctgttttcc actaaaactt tgacattagg gccagcatcc
    23881 atcgtaaagt agcatggaat gccttgcttt ctcaattgtc gcaccgcatc catcgctttt
    23941 aagctgtcag gagaccagta agtgaatgga ggctgggcag ctaaagtcgt tccatgcatt
    24001 tttagtccgt ttctttccat cgtttctccg agaagttgga aatcttttgt tttgattgct
    24061 tgtttcaatt gatatagatc cccttcaacg gaatctaacc agccttgata aaaactggat
    24121 gtttcgactg tccgacgcat cccatttctg ctggacactt cttttttctg gtcgttgatc
    24181 aaaacgaaaa ccattgctaa atcgtcttcg aaagaatcgg aagggactgg cttagcgtaa
    24241 gaacttaagt cgtcatggcc tttttcccat tcgacgaaac caccgaaaat acttcggcaa
    24301 gctgaaccag acccgcgtcg agcaaatctt gaaagagaga gatcatctaa tccaagctta
    24361 agagcagtat tgcaagcgcc tgctaaagca gctagcccgc tggcagagga ggctaatcca
    24421 gctgcggtag gaacgaaatt ctgactgatt actgaagctt ttagtgagag gccggcttct
    24481 ttacgaaaaa ggtcaagaaa ctgactgact ttttttgttg ctttttcgtc ttgcaaggtg
    24541 ccatctagat aaaattcatc taccatatag ctatcagaaa agatgacttc tgtctctgta
    24601 taaaatgcat ccaatgttaa cgaaaggctg ttgttcattg gaaggatgag ttcttcattt
    24661 ttcttacccc aatatttgat tagagcaatg ttggtatatg cgcgtgcttt gcctttaaac
    24721 ataggtatgt actcctaatc cttgtatcca agtttcagca gcacccgcat cttcaagtgc
    24781 ttgactgatt tcttgggctt ttgtttttgt ttgtgctaat gcaatcatac agccaccgcg
    24841 tccaccgcca gttaatttgg cacccaatgc cccggtatct tttgctgttt ggatcaatag
    24901 attaagaaaa tcattgctga ttgtcagctt ttccagaagt gattgtgatt catccattgt
    24961 ctgtgccaac gtttctggtg aattttcaat gattgcttgt tttgcttgct tcgtcaagta
    25021 tcctaatttt tggatcattt gtccagtttc atgaggctgg gtttcaaaga ggtgagcgac
    25081 atctttgacg gcttctcttg tttgtccttt gattcctgta tcagcgacaa tcaaaaaagc
    25141 atcgatgttc aaagaaaagt agtcgaaagg atgccctttt gtaaaataaa tcggctgcaa
    25201 gctgctagta gcggctgcat cgattccact aggattaccg tgggcgattt tttccgaaag
    25261 ctggacattt tctaatagta tttcacgaga caaaggaaat gctaagtaat cataaaaagc
    25321 acgagtgact gctgtggcga ctgcagcgct tgatcccatt ccacgttcag caggaatcgt
    25381 gctttcaatg gtcagttgca agtttttttg gatgtcatgc tgttttttta agtgaatgaa
    25441 taattttttg atatttttta atgcatgagg agcttcggct aaatttcctg aatagtagga
    25501 agaaaccagt tgatcgtcca ttgcatccag ctcttcaagg aatgcggtga cttttgttgc
    25561 atagaaagga aaggcaatcg ctggttctcc ataaacaacc gcgtgctcgc ccatcaatat
    25621 gatctttccg cttgattctc cttggccata gtttgccata atctaactcc ttctcacgta
    25681 aaagtgattt ttaagcaaaa aataaagcgc ccaaaacgag ttgcgctgct tcacgaattt
    25741 ttttgtccac taagggtctc aaaaaacatt cctcctttat tttactaaaa ccatctcaga
    25801 agtaaaacta agaatacgtt ttctaaagaa aaaacacttt tttcttaaga attttttgac
    25861 tttttttctt ctttgactag aaaaaaatac aattgacact actagatatt gggggtatat
    25921 ttaaaaaggt tggagttaaa cacaatattt agtgatgaag tgagctggag gtaaatgagc
    25981 gtgatcgttt tagcaggtac aattggtgcc ggaaaatcaa gtttaacaga aatgatggct
    26041 gaacactttg atagtcaggc attttatgaa tcgattgatg ataatgaagt cttgccctta
    26101 ttttatgcaa accctgaaca gtatgctttt ttgctgcaaa tttatttttt gaataaacgt
    26161 tttgcaagca tcaaacaagc aatgaaagat gacaataatg tattggaccg ttcgatttat
    26221 gaagattcac tgcttttcca tctaaatgca gatctaggaa gagcaacaga aacagaagtt
    26281 cgtgtgtatg atgaactgct ggaaaacatg atggaagaac ttccctatgc tgcccataaa
    26341 aaacatccgg atttattagt tcatattcgt gtatcttttg atacgatgtt ggagcgtatc
    26401 gaaaaacgtg gtcgttcgta tgaacaattg tcctttgatc caagtctata cgattattac
    26461 aaagaattga acagacgtta cgatcaatgg tatgaagaat acaaagaaag tccaaaaatc
    26521 caaatcgatg gagaccgcta taattttgtg gaagatcctc aagcaaaaga agaagtcttg
    26581 aaaatgatcg aagaaaaatt agcagagatt cgccaaacca aagtagccat ttgatttaca
    26641 gttgctcaaa taaagggtaa acctttattt gagcgacttt tttctttata ctggaaacaa
    26701 atgtcaggag gatagagtat gcgggaaaaa cactatttta atcaagaccg taatttttgg
    26761 atcaatgtac agcaaaacga tgtgctagct gttaaaaact taaaagaaga atatggcatt
    26821 agtgacgaga tgctgactta ttctctagat aaaaatgaac gggcgcgcgt agagtacgat
    26881 tcttttgatg aagcgttact gcttgtatca aatgttccgc accagcaaaa agtcgataat
    26941 cattatgaaa ctagtcctat cgcttttatt ttaaaagaag acggtttgtt tacatttacc
    27001 acgccgaata ccgagtacgt gattcgacta attcgatcgc ttttggaacg aatgccagac
    27061 atgagtgtat acagtttatt gtttcgtaca ttgtttttga tttctgattc cttttttcct
    27121 ttaatcgagg aagtgaatag tgaacgtcag cggcttaatc tgaaattaag agaaaaaaca
    27181 acgaacaaga atctgctgca actttcagat ttagagatcg gtttggttta cttagttaca
    27241 ggaacaaaac aaaatgtagt acttttagaa caaatcaagg cattggctat ttatcgcaag
    27301 ttgtcagaaa aggaaaaaga agagttggac gatgcgctga tcgaagcacg acaagcagtt
    27361 gaaatgacga acttagcttc gcagatttta gaccagctct ctggcactta taataatttg
    27421 ctgaataaca acttgaatga cacgatgaaa tttttgacgg tctggtcgct gatcttgacc
    27481 gttccaacta ttgtgacagg attcttcgga atgaatctcc agttaccctt tacccattcc
    27541 gttttcggat ggggaatcgc cttgatcatc agtttggtct tatctatttg gatgcttatt
    27601 gcactgtggc ggcggattcg ttgatctagt ctttgatctg caaaccaagg gcttgggcga
    27661 tcacaagtgc ttgctcttgg ttgacgatta gtcccgccaa agaatcttga gataaagcga
    27721 ttgctgtgaa gaaattagtc gtaaagtcta atcccttcag agaagttcga aaccagttcg
    27781 aaccggtcaa ctgattgtca gacaaaaaaa ggtttttcca agtgacctca taaaattcgc
    27841 tgtcttctaa ttgacaatga tcgaaggaga cagctttcaa gtttgtacta ctaaaggaag
    27901 ccatcgaagc aatacaatca gtgaaagtac agtctcgaag ataactttcg gcaaaattcg
    27961 tacctgttaa tttacattga tgaaacacga cttggtggaa actgccacca atccactcca
    28021 gattggaaaa atcacatttt tcaaaaatga cattactgca ttcaaaatga cgtaaaactg
    28081 ttttttgcat cgtaagtttt tggatatgac tctctcttaa taccatattt ttggcatcga
    28141 gatagctttg gtcacccccg ttgatcagaa gaccagagaa gatctcctca tcttctaaat
    28201 aaaactctcc ctttgtaaga agaggaagag aaggtggaat aggttcgatt tttttcatgt
    28261 gggttcacct cgtttttctt tagtatagca aagaagcggg aatcactgct agaaagtctg
    28321 ctaatctaat cattttcaga aaaggaagac aaactgtatg aaaaacattg acaaaaagat
    28381 tagtttttgc tatactatga tggttgagaa ataaaagcag aagcacccgc ttctcgcctt
    28441 agttgataat gtcactgggc tagatagttt tcactggttc tattaggtga agtacgcgcg
    28501 ggttctgtaa gagaactcgt ttttttattg ccgtttattc ggtaagcttt tctttctctc
    28561 ataatatttg gaggtgaatg accatagcaa aggatatgat ggttaacgac ggcattcgtg
    28621 cacgagagtt acgattgatc ggttcagatg gtgagcaatt aggagttaag acaaaagcag
    28681 aagcattgaa tattgcagaa caagcaaact tggatcttgt gttagttgcc cctggtgcga
    28741 aaccaccagt tgcgcgaatc atggactacg gtaaataccg tttcgaacaa caaaagaaag
    28801 accgcgaagc gcgtaaaaaa caaaaagtga tcaatgtaaa agaagttcgc ttaagtccta
    28861 cgatcgactt gaatgacttc aatacaaaac ttcgtaatgc acgtaagttc ttggaaaagg
    28921 gcgataaagt gaaagcttct atccgtttca aaggccgtgc cattacccat aaagaaattg
    28981 gtcaaaaagt cttgaatcgc ttagctgaag aaactgcgga tattgcaaca gtggaacaaa
    29041 aagcgaaaat ggacggacgc agcatgttct tgacgctggc accgaaaaac gacagtaagt
    29101 aatttgaatg actaactggc tgttggacag ccgatagtcg gataacgaaa gattttgagg
    29161 aggaaatata gtcatgccaa aacaaaaaac acaccgcgga tcagcaaaac gtttcaaacg
    29221 tactggtaaa ggcggattga aacgcttccg cgcgtttaca agtcaccgtt tccacggcaa
    29281 aacaaaaaaa caacgccgtc aattgcgtaa agcaagcatg gtttctagtg gcgacttcaa
    29341 acgtattcgc caacaactag caaaaatgaa ataagaagta caaataacag aatctgaaga
    29401 tttattttta ttaggaggaa ttatacatgg cacgtgttaa aggtggaaca gtaactcgta
    29461 aacgtcgtaa aagagtgcta aaattagcaa aaggttacta cggttcaaaa cacactttat
    29521 ttaaaacagc aaaagaacaa gtaatgaatt catacaacta cgcatatcgc gatcgtcgtc
    29581 aaaagaaacg cgacttccgt aaattgtgga ttgctcgtat caacgcagca gctcgtatga
    29641 atggcttgag ctactcaaaa ttaatgcacg gtttgaaagt tgctgaaatc gacatcaacc
    29701 gtaaaatgtt agctgacttg gctgttaacg atgcagcagc atttactgca ttggctgacc
    29761 aagcaaaaga agctttggct aaataagcta aacaattata aagaggaaag aagataacga
    29821 aaagttatgt tctttcctct ttttttattt acaatcctcc atcaaatgaa acaggacaat
    29881 aacgtataag tctaaaatag cataattaag gttttttaat tgaaaactga acatttatgg
    29941 tcacccttta atgaaagcgc taactacata agttagaatg agtttgactg atatattagt
    30001 ttgcttatca gaaaaacaaa atggacggag gaataacatg agtgatatta ttattggtat
    30061 tttgttagtt atctcattct ttttcatggt ctggtactgt gtaaaaggct acaacttgat
    30121 ggtcggcttt gccatcatgg ctactgtatg gatgggatta gcgctagtcg gaaacacgtt
    30181 ttcaccaaac ccggcgatgg aagggcaagg agtaattgat gtgttgacgc atatctatac
    30241 gacggggcca gctgagtacg ccaaatcgat cttggtaaat gtcttctttg gtgccttttt
    30301 cgggcgtgtt ctagtagata gcggaatcgc agctaccctg attcgaaagg tagttgaact
    30361 tggaggggac aagccaagaa tcacgatgag cttactatgt attgtaaccg ctgttatatt
    30421 catgtcgatg acaggaatcg gaccagtgat ttctattgca gttattgtgt tgccaatctt
    30481 gatgtcatta ggtatttcag taccggttgc actgttctca tttatgggct cgattatggc
    30541 tggaatcttt gctaatatcg tcaatttcaa acaatatcaa acgatttatg ctggtttcaa
    30601 tccagcggct gaaagctata cttataatga ctatttccaa attggtatga ttgggatgat
    30661 tgtaagctta gttgtggtac taacggtcgc taatatatca atgaacaaaa agaaacgcta
    30721 tgcaatggca gcaaacgtac cagctgaagg tggcgacgca ccaatgatct catggttagc
    30781 cgtattatta ccagttttag gggtcgttct tttagatctt ccaatcatct taggttttat
    30841 tctagcaggt atctgggcat tgctgtttac tggaaaattg cgcggaggtt ataaagaaat
    30901 ctgtcgtcag tttgcgaaat tatttacaga tggcgcagta gatgttgcgc caatggttgg
    30961 tttcttgatg accttagcga tgttcaataa ttcagctgcg tatgcttctc cttatttttc
    31021 agctattttt ggagattgga taccgcaatc accattagtc ttggcaatcg tatttgctat
    31081 attgacacca ctaggatttt tccgcggtcc aatgaatcta gtcggttcag gctcagctat
    31141 tttagctgtc gtattagctg ttaatccaac gatgtcgcca gcctttttgt tcccgttatt
    31201 tgcaatcaca acgattgctc ctcagcattt agatattaca caatcttggg ttgcttgggg
    31261 attaggttat acaaaagtaa cttctcgaga atatatgaag aaatccatcc caactggttg
    31321 gatcatcgga gcgatcttat gcctgatcac gttcttattg tacgggaacg cctagtaaac
    31381 aaaacgtcga taaaaacgat tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga
    31441 atgggaattg atgttggcgg tacacatacg aaagcagtcg caatcgataa tgcgacgcac
    31501 gaaatcatcg gaaaatcttc agtgaaaaca acccacgatg atgtccgtgg agtagccgca
    31561 ggtgtcgttc agtcttttca aaattgtttg ctggaaaata atatcagtcc agaagatgtt
    31621 gtgtttgttg cacattctac cacacaagcg actaatgcac tgattgaagg agacgtagca
    31681 aaagtcggcg taatcgggat ggcaaaaggt ggattagaag gttttttagc aaaacgacaa
    31741 actagattga acgatatcga tttagggaat aagaaaaaaa tagagattgt caatgcattc
    31801 ttacctgtca aacacttaaa tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga
    31861 gaaagagcgg aggtcttggt ttcttctatg gcatttggag tagataatgg ggaaccagaa
    31921 cgagttgttt atgaagcagc atcggtaaaa tcgatcccta caacgatggc ttctgacatc
    31981 acaaaattat atggattgac aagaagaact cggacagcag caatcaatgc atctatttta
    32041 ccaaaaatgc tagatacggc aacctctaca gaagattccg tacgagaagc aggggtcaat
    32101 gtttctttga tgattatgcg cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa
    32161 cgacctgttt taaccatgct ttccggtcca gcagcatcag tcatgggctc tttgatgtac
    32221 ttgagagcgt ctaatggtgt ttattttgaa gtaggcggaa caacaaccaa tatcggggtc
    32281 attaaaaatg gacgtccagc aatcgattat tcaattgtag gtggccaccc aacatatatc
    32341 tcttcattag atgttcgggt tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag
    32401 tcaggaatca tcgatgtcgg tccacgttct gcacatattg ccggtctaga ttatgcagta
    32461 tttacggaga cagaaaaaat caaaggtcct aaggttgaat ttttctctcc taaagaagga
    32521 gaccctgctg attacgtcaa ggtagtgatg gaagacggag aagaagtgac tattacaaat
    32581 acatgtgcag ccaatgtcct tggattagtg caagaagaac atttttctta tggaaatgtc
    32641 ccatctgcac gaaaagccat ccaagcatta gctgattatt gtcatacgac ggttgaagac
    32701 atcgcagaac agatcatgga aaaatcctat gctaagattg aaccggtgat tttagaattg
    32761 gcagacaagt atcacttgga gaaagatcag atttctcttg taggtgtagg gggcggcgct
    32821 gcctcattga ttacttactt tagtaataaa atgggtgtga aatattcgat tcctgaaaac
    32881 gccgaagtga tttcttctat tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt
    32941 atcatccctt cgccaagtaa agaagatata cgatcgttga aaaatgaagc gatgaataaa
    33001 gcgattgaat caggcgctac accagaatcg atcgaagtcc acgtagaaat cgatccacag
    33061 acttcaaaag taacggctat cgctactggt tctacggaag tgaaagcaac cgatctaacc
    33121 aaggagatca caacagaaga agcactagag cttgctgcag aggatatgcg attaaataaa
    33181 aatgaagttt gcttattgga aaatacacct ttcttttatg tatgcggcga acagaatcgt
    33241 tcaaaaaatg ctggaagtct tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt
    33301 ggacatgctt catgtatgaa aacaactgca gccaattata tgacagctgt cgaacaactt
    33361 tgggaagata tggcagttta tcaaactgaa ttgattgccc gaccagaatt ttatctctgc
    33421 ttaggagcaa gagtctctga ttttacagcg acggatctgg aacaactaca gcttttgatg
    33481 gatctggaag tatctactat ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc
    33541 aaacaaacat aagttagaag agttactaaa cgaattacaa cagatccccg aagaaatttg
    33601 gggattctac cagtttcggc gagatttatt ttggaaaaag attcctttgt ccaagcagaa
    33661 aattcttata caacaatcaa tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa
    33721 gtaccctttt gccgatgtag gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg
    33781 tgaatcagag caaataaacg aacgaatcac ttttgcaact tatgcagaag atgaagggat
    33841 tcgattgatg acggagccgt tagaaaaatt aaaatgttca ggactagctt cgatctctaa
    33901 agaaacagcg caagctttga ttattggaca tgaactattt caccacatcg aagcttctgt
    33961 taagggaatt tatacgcaaa gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca
    34021 tcaatcaaac attcgtgcat tgagcgagat agcagcgatg agtttttcaa aagagatgaa
    34081 tcaaagcgat ttttcgcctt atgtattaga ggcagtctta ctttggccat ataatgaaat
    34141 tcgtagccaa gggatcttag aagaaataaa aaaaatagaa aaaagatgta cggagtatga
    34201 tttagattac aaataaagag actgtgacaa aagtcgtgtc acagtccttt ttttccgaat
    34261 aaacggtgtt ctcctgtcaa ctcctcggtc acaagtcaaa atattcaaca aacatgagaa
    34321 gctgtttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttg
    34381 gaaaacggtg ttcaaaagct tgcatctgcg gtaataagtc cagccaagtt aaaaacataa
    34441 gaagctattt ttacttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc
    34501 tcaataaacg gtgtgcaatc agctgaccct cggtattagc tcaaaaaaaa cggcaaaata
    34561 tgaggaacta ttttgccgtt tctttctcta atactcaggt caaaacgctg attccaaaac
    34621 ctcttatttg atgggtctgt cctgaatatc ttcaaaataa tcggggtaat tatgaatcac
    34681 gtattcttgc tcctcgataa tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc
    34741 aaagttatga tcaatcattg attgaagcat tgtctgatgt tcatctaata ctcgacccca
    34801 gtctaattct gaaatcgtca aacgtaacca tctgaaacgg ttcaagtgcg tactataact
    34861 ttctagccaa tcccaaattt ctcgttttcc agcaatttca taacaagttc ggtgaaaatc
    34921 agtatccaaa tcaaaaaaag cttttttatt ttgagcgatt actgcctgat cggtttgttc
    34981 tagtattttt tctagtttag cttgatcttg cttggtcatt ttcgcactgc attcttgcag
    35041 gatcggtttt tccaactttt cccgcgtata acgggctaat gaagcagatc ggatatcgat
    35101 ttttgaaaca taagtgccgg actgtggaat ggtttttaca agctcttgat tgcgcagttg
    35161 gatcagtgct tctcgaatcg gtgtacgccc gatagctagt tgttcttcta acactttatc
    35221 ggagatttta cttccaggtg ctaactcgga ataaatgatt tgtttacgta tagattcata
    35281 agcttgctgt tgcaaattat tgataaccat ttaaccttcc tttctatgca gatataatct
    35341 attataacat aaatcgctta caaatcgtgt gggcataaaa acacagtctg caaaaaaagc
    35401 cctgataggc atgcctatta gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac
    35461 ctcttggatc ggtttgacac gaccgccgat tcttttagca aaagcttcgg attctttttt
    35521 gtctttgaaa atgagcagat tgtctaagtt tttttcccga gtgcgacagc cagttttatc
    35581 gatgtaaccg tgcattactt taacgacata catggggatc acctgctttt ttcttttcat
    35641 tatacgaact actcgacaca atacaagaaa aatgcttaga cggtctttat taagaattta
    35701 catcatccaa agaagcgtac gcttggaata actgatctaa ctccggattg actttgctca
    35761 gcttcactaa tcgttgcgaa tcagaaagta aaaaacagtg tttactactg ccagtagttt
    35821 ttaatttttt gtcttcagtt cccacaattt cgtagctaaa atccaaccgt gtccccgtat
    35881 acttttggat agttggatgg atgctcactt tttctccgta atggatcatt tctttatagc
    35941 tgcaatttac ttccagaacg gggatgatga tcccagattc ttcgattttt ttatagggaa
    36001 accctagatg taccaataag gctacccgag cctcttcaaa ccagcggata taattgctgt
    36061 gatgaataat ccccatttga tctgtttcat aataaaaagg ttctcgcaca taacctgtaa
    36121 atttctccat gactcatgcc tcgttttcca ttgatttttt acaaaaataa ttcatccatt
    36181 tcggaagtgc ttcgctgatt tgtgtcggca aaacaatata ttgttctttt gccagctggt
    36241 ctccaatgaa actatgaaga taaatagctg ctgcaatcgt ttggtcattt tgaggaaact
    36301 gggctaaaaa tcctgtgatc atcccagcta aagtgtcacc catgccgccc gttgccatag
    36361 cagcagaacc taaaggattg gtgtagaagt gctggtgacc ataaattttt gtctcatgac
    36421 ttttcaaaac gagcacagca ccgatttcct gttgcttctt tttgttttct ttatcccgtt
    36481 gttcttgtat cggtagagaa ctcaaccgtt gccactccat ttgatgaggt gtaagtacag
    36541 tttgttcggg atagggtagc aaaagatgat tagcagcaag taaacttaaa gcagagccat
    36601 caatcaccag ccattgttct tttgattggt tttctagtac ttgtttgaaa cgttccaaac
    36661 tcttagaaga agtacccaat ccgggaccaa tcaagagtac atctgcttgc tttattacgg
    36721 aaagaaactg gtcagtatcc gtccaaacta caatcattgc ttctggcaga tgactgtgaa
    36781 gagcactccg atttttttca tcagttacta cagtcgtgag acccgctccg ctgtgtacgg
    36841 cagctaaagc actcataatg attgctccgc catattgttc attgccgcca atcaaaacga
    36901 tacgtccaaa tgttccttta tgtgaaattt ttggacgagg tgtgatgact cttttcacta
    36961 gttgttcgtt tatcagcatc taaaaacctc cttttactca ttatagcgta tatttttttt
    37021 agagaaaaac agataagaaa aggaattgtc agattttttg aactattttt ttctgtaatt
    37081 tgtggtatca tgaacacgaa gacttaaata agtggaggat taactttatg acaattgatt
    37141 ggcaaaaaga agtagaagca cgtaaagaag acttgcttga agacttgaaa aatcttttac
    37201 gtgtcaacag cgagcgcgat gactcaaaag taacaccaga tgcaccattt ggacctggac
    37261 ctcgcgacgc attgaaacat atgttagctt atggcgaacg tgatggattt aaagtaaaaa
    37321 atgtcgataa ctatgcaggg catattgatc ttggcgaagg agacgaaacg ctagggatat
    37381 ttggacacat ggatgtcgtt cctgcaggag atggctggga cacagatcct tatgaaccag
    37441 taatcaagga tggaaagatc tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg
    37501 cagcttacta tgcgatgaag atcattaaag aacttgactt gaaactatca aaaaaagttc
    37561 gcttcgtagt cggcagcgat gaagaaagcg gctggggcga tatggcttat tatttcgaac
    37621 atgaagaaga accagatttt ggtttctcac cagatgctga attcccaatc atcaatggtg
    37681 aaaaaggaaa tgtttcttta gctttacgct tcaaaggcga caatgcagga gattatgtct
    37741 tgaaatcttt tgtttccgga cttcgcgaaa atatggtgcc aggaacagca actgcagcac
    37801 ttcaagtacc aagtgcagat gcagctatcg cgatggaaga agcattttac caattcatcg
    37861 aagcaaatcc agtaagcgga acgattgaag cggacaatac gtatgtaaaa atcgaattag
    37921 ttggtaaagg cgcacatggt gcaagcccac aatctggtat caatgcaggt tctttcttgg
    37981 cattgttctt agataactat gaattcctag gatctgctaa acaatttatc catgtagcag
    38041 ctgcttatgt ccatgaagat ttttatggag aaaaactagg agtcgcttat gaagatgaaa
    38101 aaatgggcaa attgacaatg aatgctggac tatttgcttt tgaagaaaat ggtacagagg
    38161 aagcgaactt catcaatatg aatttccgtt ttccaaaagg tgtaacagta gacggattgc
    38221 aaagtgacat cgaacaaaca gtggggcaag aaggcgcaac agttactcga ggtgctcgcg
    38281 taatggaacc acattacgta ccaatggatg atccattggt ggctacatta ttgcaagtct
    38341 atgaagacca cactggagaa aaaggctacg aacaaatcat cggtggcgga acttacggac
    38401 gtttgttgaa acgtggcgtt gcttatggag cgatgttccc aggatatact gatacaatgc
    38461 atcaagcaaa cgaatttatg agtcttgatg acttgttcag agcaacagca atctatgcag
    38521 atgccatcta tcgtttggcg aaataattaa ttaaaaagag gatcaagaat tgcaaattat
    38581 ttttgcagtc ttgatccttt ttttactttc tactgtggta ttcatgataa acatgatata
    38641 gattcaattc atattctttc ttagcgttcg tgacgactgt atccaaaatc aaccctgtta
    38701 ctaatgaaag aagtgctaaa gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg
    38761 taataaagct tgtcagcata tattcgcgaa tcacaggaat ccccgtgacc aatccaaata
    38821 aaaagaagaa gatggtccaa atcccaaaga aaagcaaagg cttataatct ttaaacattt
    38881 taataatcat catgatcact ttgaatccgt cagagaatgt attcaatttt gattcgctgc
    38941 cttcaggacg atcacgataa tcgattggga tttcaacgaa cttgaatttt ttatctaaag
    39001 catggatcgt caattccgtt tcgatttgaa aaccagaact cataatagga aaacttttga
    39061 caaagatacg attgaatcct cgatagccgg tcatgacatc aagaattttt gttttataca
    39121 ttctagtaat tgtatttcta acaaggttat ttccaaaatc gtggaaaggt ctcttattct
    39181 catcaaagta ggttccattt gataggcgat caccaatgac catatctgct tcaccagaac
    39241 gtaatttctc gagcaaacca tgtacagctt ctgcaggata agtatcatcg ccatctacca
    39301 ttaaataata gtcggcatcg atatcaaaga acatttggcg gatcacattt ccttttcctt
    39361 gacgaggttc tttttttacg attgctccac cttgaacggc caattcgtat gttcgatcgg
    39421 tcgaattatt atcatagaca taaatatctg cttctggtaa ttctcgttta aagtctgcaa
    39481 tgactgttga aattgttgct tcctcattgt aacaaggaat cagaacagca attttttctt
    39541 gttgttccat ttttcactct ccttaactat agaaaacaaa agctaaatta aaaatacctc
    39601 ataatcaaag gaaatgctag tttttcccgc taaatatcat ttatttaaaa agaaaatact
    39661 ggaatatatg tttggaaagc tctatctaaa aaaccaccca ctacaaaaga aggacatgat
    39721 acctctcttt tgaatagtgg gtgcgtattc gttctctcac ttttttgacg tattaatctg
    39781 ttgcaccttt ttttcttgaa atttctaata cggttcctgt atgagcatct gctaaaaatt
    39841 cataataaac agtttcgcca tcttctattc gagaaagacc tccccgataa gcactcgacc
    39901 gaacggcgaa tttcctaata ggttcctttt caaaagagat ccaagaacct tcgattgcac
    39961 cttcttttag aaaagatgac ttgatatttt caagtacttg gtctgctgac aatacttttt
    40021 tgcgatgata ccaaatcgtt gaagctaccc cgctcaataa tcctagaccg attccaactg
    40081 ctaacccgcc tttaaaatag cttagttcgc tttcttcata catgtggaca tacctccttc
    40141 ttctcacttg ttactccttg tgcttatttt accacactct catattttct ttgaaataaa
    40201 taaccgctac cagaaaaaat aagctataat aaatacgatc ggtctgcaga aaaaatcata
    40261 ttttatccgc agatcaatat aagtctgaga ggagtttagt ctgaaattta gacgaatagc
    40321 atatggaaga aaaaacattt caacgaatca aagagttaac agaattacaa ggaacaagcg
    40381 gttttgaaca agatgttcgc gcttatatgg aaagtcatat ggaaccgctg gtagatgagt
    40441 tacagttaga tggtttgggc ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac
    40501 cccgtgtaat ggtcgcagca catatggatg aagtcggatt catgctgaca caaatccaag
    40561 aaaacgggct ttttaaagtc gtacctctag gcggctggaa tccttacgtt gtttctgctc
    40621 agcgttttac acttaaaacc tctactggta agaattatcc atgtatttct tcttccgttc
    40681 ccccacattt attgcgagga acaagcggac aaaaatcagt agaagtaacc gatattttgt
    40741 tcgatgctgg gtttgaatca agagaagagg caatgagtta cggtgtcctt ccaggagaca
    40801 caatcgttcc ttacgctgaa acgatcaaaa cagccaacgg aaagaatatt atcagtaaat
    40861 catgggacaa ccgctatggc tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg
    40921 aaacattagg tcatacattg attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg
    40981 gttctaaagc ttctgtcaat aaattcaagc cagatctgtt ctttgcagta gactgctctg
    41041 cagcagatga tacagtgacg aaaaatggca cattcggaca tttaggagaa ggtacattga
    41101 tgcgtatcca agatccaggt ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata
    41161 tcgctgaaac aaacaatatc ccttatcaat actttgtttc aaaaggcggt acagatgcag
    41221 gtgctgctca tacacaaaac gaaggaatcc caagtacagt gattggcgta gtgggacgtt
    41281 atatccatac gcatcaaacg atgttcagta ttcgcgattt tgaagctgca cgagaaatgt
    41341 tgattcaaac actgaaaggt ttggataaat caacagtaaa tacgatcgta tacggaaaat
    41401 aatgcgatag tgaaactata aaatctggca aaagattgct ttttgccaga ttttttttat
    41461 aaaaagagga tcaatgtttg ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac
    41521 ataaacagaa agtagaggac aaagaatgat tatcccaaat agtttagaag aattagcagg
    41581 ctacgtagaa actggaaaga gtgtattttt ctttactgcc ggctggtgcg gagactgccg
    41641 ttttatcaaa ccgcaaatgc ctgaaattga aaatgacttt tctgattggc aatttattga
    41701 agtagaccgt gacaagtaca tagatgtagc cgctgaatgg aacatttttg gtatcccaag
    41761 ttttgtcgtg atccaagacg gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac
    41821 aaaacaggaa atcgaatcgt tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg
    41881 ggtcaagatg aatcaagaat ataagaatat tttagtaggt atcgacggta gtgaacaagc
    41941 acttgctgct ttcaaaaaag cggtagaagt ggctcgccgt aataatggga aagtatatgt
    42001 agcaaacgta atcgatcagc aattttataa ctttatgggc tatgcaccta ttgatcaaaa
    42061 tctagtcgat cagcaaactg aaacagccaa ggaaatgatc gaagaatgta aagaatatgg
    42121 gaaatctgtt gattacacaa atatagaagg aattattgca tatggttctg ttcgtgaatc
    42181 catggcagtc aaattgccaa aaaaatatga gatcgatcta atcatggttg ggcagtcagg
    42241 tctaaatgca gtcgaacgct ttatgacagg aagtgtagcc agctacgtga tacgtcattc
    42301 accttgtgat gtcttgatcg tttctgacga agaagaacag ccaagtaatt aagcaagtgg
    42361 aacttgtacg acctttttaa tcaattaact aaaacagtaa agggactgtg acaaaagtct
    42421 tgtcacagtc ccttgttacg aataaatggt gttccagaag aggcttcttc ggaaataaag
    42481 catgaaaaaa atttcatgca gtgaatgcaa ttttttgtta agataaatct cagcaaagga
    42541 taatggagga aagaacatga tttttgctta taacaaagca cacgtcggag acaccttgat
    42601 ggttattata gccgacgata aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc
    42661 tcggatcaaa gatgaacaag gtcaagttgt tgcttggaac tttttccata tctccgatca
    42721 tctaacaatc gaagggaacg gccaagtaag cttaacagat gaggaattag cgaaattgaa
    42781 tcgattgatc aaagaagcag gattcgaaga acagcttgaa gctgatcatg aaccgaagat
    42841 cgttgtcggg tacgtaaaaa catgcgtccc acatcctgat tctgaccatt tatcgattac
    42901 tgagacagaa gtagacaatg gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga
    42961 agctggacaa aaagttgtcg ttgcaaaacc aggagctatg atgccagatg gaatgatgat
    43021 ctggccagga actttgcgag gggtagatag ctatggaatg atctgttccg ctcgtgaatt
    43081 gcatttgcca aatgctccag caaaaaaagg aatcttagta ttgcctgagg atgccgtagt
    43141 cggagaagca tttccaaaat aaagaggttg tgacaagtct ttgtcacaac ctctttttta
    43201 ttaattttca ctattttgat tttgttttaa tgccgattga tcgatcgtca attcaacagt
    43261 agttgttttt tctttcgagc cttcatagta agtgattttg acagaatctc ccactttttt
    43321 cttatacaaa gcagattgaa gttctacacc agaagaaact tctttgtcat cgatcttagt
    43381 aatgacgtcg tattgtttca atccagcttt ttcagccggt gtagctgttt ggacatttgt
    43441 cacgatgaca ccgttagtta cagatgaagg aatcttcaag atttgttctt gttgctgagt
    43501 agaaacggca gataagtcga ccattgtgat acctagagct ggacgtgtca cttttccgtc
    43561 tttttccagt tgattgatga tattcactac atcgttactt ggaatagcaa atcccattcc
    43621 ttctacgctt acattagaag tagattcaga agtacttgcg atcttacttg agttgatacc
    43681 gataacttgt ccttcgatat taactaaagg accaccagaa tttccagggt tgattgctgc
    43741 atctgtttgg atcgcattga tattgactgt ttcgttcgat tcattcgtac ttgtcacttg
    43801 tcggttcaag gaagaaatga ttcctgaagt gacagaattt gcatattcag atcctagtgg
    43861 ggaaccaatc gcaatcgcag gctcaccaac cttcagagca cttgagtcac caaaagaggc
    43921 aactgtttcg actttgtctg aattgatctt caacaccgct aaatctgaat aagcatctgt
    43981 tccaactaat tcagctttta ccttcgtacc gtctttcatc aagacttcaa gaccttgctg
    44041 gccatctact acgtgattat tcgtgactac ataagcagta ttcccgtctt ttttgtagat
    44101 cacgccgctt ccttcactat aagcttccaa attactatca tctgagctgc tactttcttc
    44161 ttgctgcccg aatagtccgc caaaaccgct tgattggttt tgactttgta agttgataac
    44221 agaaacgaca gcgtcttgaa ctttatctac cgctttggtg atatcagaat ctacattgac
    44281 tttgacattt tccacaactg tttcaccggc agaattctga ttgccgcttg tagcagtaga
    44341 actattgttg ccagtgccca tcgctgcata aaaaatgcca gctgtcagta gtcctccaac
    44401 gatccctcca acaagcccca gacccaactt acgccagagg ctattatttt tatttttttt
    44461 catctttggt gtcacatttt ttctatccat gtgttcctcc tccaattaca ttcatttatc
    44521 tactgttagt atagtcttat ttgttgactt tagtctactt tgagactttg aactttttat
    44581 gaaaaacttt aggaaaaatc attgttttca gatgaaaata tttgattttc tttttccaca
    44641 gatattgtgt aaaactacgc ggataggatt ggaaaaataa gggataaaac atatccacag
    44701 cctgtgtata aagtggataa attcttataa aacgtatgtt tgcctaatga atgtgatgaa
    44761 ataaggtaaa acaacgctgt tttctgtgga taaacctgtg gatgttgtgc ataactgttg
    44821 gggaaatctt tttaacaaaa atagtcaaaa taaacatata gacgaaaaaa ttgattataa
    44881 taaacaaagt gagcgtgaag atcaaaggag agaaaacatg aatatcaaaa ttatttcagt
    44941 aggaaaatta aaagaaaaat acttgatcca aggtatcaat gagtatgtca aacggctgaa
    45001 tgcctatgcg aaaatcgaat tgatcgaagt tcctgacgaa aaagccccgg agaatttaag
    45061 cgaagcgcaa atgagacaag taaaagaaaa agaaggagaa cggatcttag cgaaaatcaa
    45121 agaacaagaa tacgtctatg gcttagcaat tgaagggaaa aatcccacaa gcgaatcttt
    45181 tgctaaacaa atcgaccaat taggtatcca aggaaaaagc caccttgttt ttgttatcgg
    45241 cggttcttta ggactcagcg aagccgtcat gaaaagaagc aatgctcaga tctcttttgg
    45301 caaaatgacg tatccacatc aattgatgcg cctgatcctc gtcgagcaaa tctaccgtgc
    45361 cttccggatc aacgcggggg ctccatacca caaataatga tattttttaa gagaaaaata
    45421 gcataaaaat ctagttatcc gcataaaaac tggacttatc acactttatc aaggtcaaaa
    45481 ccactcaatt tactactaat ttactactta tgaatgagct ttgatacgac gatttatcct
    45541 tgaaaagtga agatataaag atacttccaa taaaatttga atatttaata ggtagacact
    45601 tcaaaaaatg aggtgtctat ttttttaccc gattttgaaa ggaagtgaac ttatgaaaac
    45661 aaaaaatcaa gaatcaaaag gtcgttcccc actctttaag accatcaaac attcattcag
    45721 ccaataaaaa agaaaggata ggtaaaaata tggaacttaa atttgtgatt cccaacatgg
    45781 aaaaaacatt cggcaattta gaatttgctg gcgaggataa agtcgttcag cgaagaatca
    45841 acggacggct aactgtctta tcaagaagct ataatctcta ttctgatgtt caaagagcag
    45901 atgatattgt ggtggtgctt cctgctgaag ctggcgaaaa acatttcggc tttgaggaac
    45961 gtgtgaagtt agtcaatcca cgtattaccg cagagggcta caaaatcggc actcgtggtt
    46021 ttacaaatta ccttttacat gctgacgaca tgataaaaga ataaagaaag agaggaaaaa
    46081 tgatgagatt agcaaatggc attgtattag ataaagacac gacttttgga gaattgaaat
    46141 tctctgctct acgtcgtgaa gtgagaatcc aaaatgaaga cgggtcggtt tcagatgaaa
    46201 tcaaggaacg tacctatgac ttaaaatcca aaggacaagg acgcatgatt caagtaagta
    46261 ttcctgccag cgtgcctttg aaagagtttg attataacgc acgggtggaa cttatcaatc
    46321 ccattgcgga caccgttgct actgccacct atcaaggagc agatgttgac tggtatatca
    46381 aggcagacga tattgtgctg acaaaggatt ctagttcatt caaagctcaa ccacaagcaa
    46441 agaaagaacc gacacaagac aaatagtcgc taggtagaaa ggagactttt tcgcatgaaa
    46501 cagcgtggta aaaggattcg cccatctggt aaagatttag tctttcattt tacgatagcg
    46561 tcactcctgc ctgttttcct gctggttgtc ggactgtttc atgtgaagac aatccagcag
    46621 atcaactggc aggattttaa cctatcacaa gcagataaga ttgacattcc ctatttaatt
    46681 atcagtttca gtgtcgcaat tcttatctgc ttgctggtag cgtttgtatt caaacgggtt
    46741 cgctatgata cggttaaaca actttaccac cgtcaaaaac tggcaaagat gatacttgaa
    46801 aacaagtggt atgaatctga acaggtcaaa acagagggtt tctttaaaga tagtgctggt
    46861 cgtacaaagg aaaagataac ctacttccct aaaatgtatt atcgacttaa aaatggcttg
    46921 atacagatac gggtggaaat cacgctggga aaatatcaag accaactctt acacttggaa
    46981 aagaaattag agagtggctt gtactgtgag ctgacggata aagagttaaa ggattcctat
    47041 gtggaatata ctttgctcta tgacaccata gccagtcgta tttctattga tgaagtagaa
    47101 gctaaagatg gtaaacttcg cttaatgaaa aacgtatggt gggaatatga taagctccct
    47161 catatgttga ttgctggtgg tacaggtggc ggtaaaactt actttatact gacactgatt
    47221 gaagccttgc ttcatacaga ttcaaaactg tatattcttg acccgaaaaa tgctgatctt
    47281 gcggacttag gttctgtgat ggcaaatgtc tactatagaa aagaagactt gctttcttgc
    47341 attgaaacat tctatgaaga aatgatgaaa cgtagtgagg aaatgaagca gatgaagaac
    47401 tataagactg gcaaaaatta tgcttactta ggtctcccgg cacacttctt aatctttgat
    47461 gaatacgtcg ctttcatgga aatgctggga acaaaagaaa acaccgcagt tatgaataag
    47521 ctgaaacaga ttgtcatgtt aggtcgtcaa gctggcttct ttctaatact ggcttgtcaa
    47581 cgtccagacg caaaatattt aggcgacgga atccgtgatc agtttaattt cagagtggct
    47641 ttaggtcgta tgtctgaaat gggctatggc atgatgtttg gcagtgacgt acaaaaggat
    47701 ttcttcttaa agcgaatcaa aggtcgtggc tatgttgatg taggaacaag tgtcatatca
    47761 gagttttata ctccccttgt accaaaagga tatgatttct tggaggaaat taaaaagtta
    47821 tccaacagca gacagtccac gcaggcgacg tgcgaagcgg aagtcgcagg tgtggactga
    47881 tcttgctggc tggtgtggca atagccacgc cagcacttaa cccctcggta tctaacaggg
    47941 gggtacaaat cgacaggaaa cagtcaaaaa aacattagaa aatcctttgg ttacaaggga
    48001 tttacaaaat ttcagcgtat gtcaaatggg ctttaaaagt tgacatacgc ctttttgatt
    48061 ggagggattt ttactgaatg aacaaacttg gttacagcat ttaaaagaaa aacgcttggc
    48121 ttatggacta tctcaaaacc gtttagctgt tgcgactggt attacaaggc agtatctaag
    48181 cgatattgaa acaggaaaag tcaagccatc agaggattta cagcagtccc tttgggaagc
    48241 tctggaacgc ttcaatcccg acgctcccct tgaaatgctg tttgattatg taagaattcg
    48301 ctttccgaca acagacgtac agcaggtggt cgaaaacatc ttacaactga aactgtccta
    48361 ttttcttcat gaggactatg gtttctattc ttattcagag cattatgctt taggcgacat
    48421 attcgtcctt tgctcccatg aactggacaa aggagttctg gtggaattga aaggtcgtgg
    48481 gtgcagacaa tttgaaagct atcttctggc acaacaaaga agctggtatg agttctttat
    48541 ggacgttttg gtggctggcg gtgtgatgaa acgccttgac cttgccatta acgataagac
    48601 agggatttta aatatccctg tactcactga aaagtgccaa caggaagaat gtatctccgt
    48661 cttccgcagt tttaaaagct atcgcagtgg cgaactggta cgcaaagagg aaaaggaatg
    48721 tatgggaaac accctctata tcggttcatt acaaagtgaa gtttatttct gtatctatga
    48781 aaaggactac gagcagtaca agaaaaatga tattcccatt gaagacgcag aagtaaaaaa
    48841 ccgttttgag attcgattga aaaatgagcg tgcctattat gcagtccgtg atttactcgt
    48901 ctatgacaat ccagagcata ccgcctttaa aattatcaat cggtatatcc gttttgtaga
    48961 taaagacgat tccaaacctc gttctgattg gaaactgaat gaagaatggg cttggtttat
    49021 tgggaacaat cgtgaacgat taaaactaac cacaaaacca gagccttact ccttccaaag
    49081 gacgctgaac tggctatctc atcaagttgc cccgacctta aaggttgcga ttaaacttga
    49141 tgaaatcaac cagacgcagg ttgtaaaaga cattctcgac catgcgaaac tgacagaccg
    49201 acacaagcag attttgaagc aacagtcagt aaaagaacag gacgtgataa caacaaaaaa
    49261 ataactcaaa tacaaattca ttgaatatag agaggagaac atttttatga attttggaca
    49321 aaacctttgt gcgactcgtt ccttagtgaa aagctaaggc actaataaaa gaacgaaaaa
    49381 gtttggttat taggagaact gataatctga caggtggaat gatgaagtaa cgctctgaaa
    49441 cgcctgccct gagcctccga caagcattgt atgaggaaac tcagaatgtt tgaagctcgg
    49501 taaagacggc tgaaagatac cgtaatagtt gatgaacata tatcttctat cgaaaactat
    49561 ccagaggtgg gtagcgtatc caacaggtcg ggggtctata aaatatctat ggttagaatg
    49621 ttctataagg aactgacgaa acagcgaatg tacgggtcta aatttgaact ttgtagaaat
    49681 gcaaaggtac gagaaatcgt agctggtgtg gcaaagtaag aatataggtt atgaaatgcc
    49741 atataacgtt acaggcggta tcaagccagc aggcttatag ctagtgacct aaggatatat
    49801 atgtatagat aagattatcg gaacgaggaa aggtattggg ttctcatcaa gagaataaac
    49861 tgacgaagaa caataagcag ttgaactaat gctgaaaagc agagggcgaa cctaagaaat
    49921 tcttgtaatg agaatggagg aatgccctca agtcgtgtat atattagaaa agtattatgt
    49981 tgtttcaata aggattacga gtaagataaa aaagctcact caatttcaag taaaggagtg
    50041 aagccttatg cctaaaaata aaaatgaaac attgtgtgta gaagatttaa gacacgctga
    50101 atattacgaa atgcaaaaca cctttgatga tttatatgcc aaaagtaaga atggagatat
    50161 tttcactcat cttatggata ttatcctatc gagagaaaat atattactgg catatcgaaa
    50221 tatcaaagca aatgcaggca gtaaaacagc aggaacagac ggtacaataa ttaaagatat
    50281 tggcaaattg ccagcagaaa cagttgtcaa aaaagtaaga tacattgttg caggcagtcc
    50341 acatgggtat cgacctaagc cagtgaggcg aaaagaaata ccaaaaccaa atggtaaaac
    50401 cagacctcta ggtattcctt gtatgtggga caggcttata caacaatgta taaaacaagt
    50461 attagaacca atctgcgaag ctaaatttag tgaaaacagc tatggtttta gacctaatag
    50521 gtcagttgaa aatgcaataa aagcaaccta taaccgccta caaatcagcc agctacatta
    50581 tgtgattgaa tttgatatta agggtttctt tgacaacgtg aaccattcca agctgataaa
    50641 acagatatgg gctatgggaa tacgggacaa gcacttgata ttcattttaa aaaggatatt
    50701 aaaagctcca atcaaaatga ctaacggtac aataacatat ccagagaaag gaactccgca
    50761 agggggaata atatccccac tactagcaaa cattgttctt aatgagttag accattgggt
    50821 agaaagccaa tggcaggaaa atcctgttac aaaaaactat gtggttcata tcaataaaag
    50881 cggtagccca tgtaaaagca acgcttacaa agaaatgaag aaaacaaagt taaaggaaat
    50941 gtatatggtt cgttatgcag atgattttag agttttctgc cgctacaagg aaagtgctga
    51001 aaaagccaaa atagcaatca ctcaatggat tgaacaacga ttaaaattgg aagtatcaca
    51061 agaaaaaaca agaattgtaa atgttagaaa aagatactcc gactttcttg gtttcaagat
    51121 taaaatgata ccgagaagaa agaagttagt tgttaaatca catataagtg ataaacaatt
    51181 caaaaaccaa aaagacaagc tggtatcaca ggcaaagaaa attgcttctc cctcaaaagg
    51241 gaaaacggaa ttagaagaaa caagacttta taattcaatg gtattaggta tgcaaaatta
    51301 ttatcagatt gcaacgaatg taaatctgga ttgtatgaaa ctgaatagag ctgtaatggc
    51361 tatcttcact aacagattag gtacaaacaa aaggggcaga ttgagaaaaa caggcagaca
    51421 acttacgaca aaggaggcaa caagatatgg taaatctaaa atgcttcggt atgttgctgg
    51481 cgagggcgaa cctattttcc ctgttggata tatccaacac agaaaaccaa tggcaaaggt
    51541 gtatttagcg aattgctaca ctctagaggg aagaaaattt atccacacca atctaagtgt
    51601 tgataaatta ctgatgtacc agttaatgaa attatctttg gaaaatcgca caatagaata
    51661 cgcagacaat cgaatttcgc tattttctgc tcaacatgga aaatgtgcta taactctgga
    51721 agaatttcaa caagctaatg aaatacattg tcatcatatt gtaccaactg gagctggtgg
    51781 aaatgacgat tataaaaatc ttattttggt aaaagaggcg gtacaccgtc taatacacgc
    51841 taaaacagaa gaaacaatac aaaaatatat agctctattg aagctaaata aagtccagct
    51901 cataagacta aataaatata gagtattagc tggcaatcaa gaattaaact tgatataggt
    51961 ctactacata aatacttttc aatataaaca cgatggaacg cggagtgcgg tgaaagtcgc
    52021 acgctccgtg tgaatggggg gaaaagtttg agatatttat aatatcagga acttacctat
    52081 ccatatataa ctggtttcta tcaaacgctc aatcactggt gcttttagca atcgttgtga
    52141 ttggcttgta tcttggcttc aagcgtgagt ttagcaaact gattggcttt ttaattattg
    52201 cgattattgc ggttggctta gtcttcaacg ctgctggagt aaaagacatt ttactagagc
    52261 tattcaatcg cattattggt gcttaaataa aaccgttctt ttgtggaata taagtggttt
    52321 tcttatgttc cgcaaaggaa tggtacacca aacgaagtgc ggtagggatt tttgaatctc
    52381 tacaaagaaa ggacgtgaat atatggacga tatgcaagtc tatattgcga atttaggcaa
    52441 atacaatgag ggcgaattgg tcggtgcgtg gtttaccttt cccattgact ttgaggaagt
    52501 caaagagaaa atcggcttga atgatgaata tgaggaatac gccattcatg attacgagtt
    52561 accctttacg gttgacgaat acacttctat tagcgaactc aatcgcctat gggaaatggt
    52621 atcggagttg ccagaagaac tacaatcgga gctatctgct ctgctcactc atttttcaag
    52681 tattgaagaa ctaagcgaac atcaagaaga tattatcatt cattccgatt gtgatgatat
    52741 gtctgacgtg gcacgctact acattgaaga aacgggtgct ttaggcgaag tgccagccag
    52801 tcttcaaaac tatattgatt atgaatccta tggtcgggat ttagaccttt cgggaacgtt
    52861 tatctcaacc aatcacggga tttttgaaat cgtctattaa atctgtcggt acattcttac
    52921 tggcagattt tcttgtttac ggggtagtca aaagactatc cccattttta tgaaaggatt
    52981 gattacatga agaaaatacg aagctatacc agtatctggt ctgttgagaa agtgttgtac
    53041 tccatcaatg actttagact tccgtttccc ataaccttta cgcaaatgac atggtttgtc
    53101 gtgtcactct ttgcagttat gatacttggc aacttgcccc ctctttctat gatagaggga
    53161 gcatttctca aatactttgg gattcctgtg gctttcacat ggtttatgtc tacaaaaacc
    53221 tttgatggta aaaagcctta tggatttttg aagtctgtca ttgcttatgc actgcgacga
    53281 aagctgacct atgcaggaaa aaaagtaacg cttggcagaa accagccaca agaagccatt
    53341 acagcagtta ggagtgaatt ttatggcata tccaattaaa tatattgaaa acaatctggt
    53401 ctggaataaa gatggggaat gttacgctta ctatgagctt gttccctaca attactcatt
    53461 tctaagtccg gaacagaaaa tacaagtaca tgattctttc agacagctta tcgcacaaaa
    53521 tcgtgatggc aagattcatg ctttacaaat cagtacagaa tccagcatac tttctgcaca
    53581 agaacgttcc aaaaatgaag tcactggaaa gctcaaaacg gttgcctatg acaaaatcga
    53641 ccaacagaca gacgctttaa tatccatgat tggcgaaaat caagtggact accgtttctt
    53701 tatcggtttt aagttgcttc tcaacgatca ggagttttct atgaaaagtc ttaccgttga
    53761 agcaaaaaat gcttttactg attttgtcta tgatgtgaac cataagctga tgggcgattt
    53821 tgttagtatg agtaatgatg aaatcctgcg ttttcagaag atggaaaagc ttttagaaaa
    53881 taaaatctcc cgtcgtttca aaatccgaag attagataag gatgacttcg gctatctgat
    53941 tgaacacctt tacggacaga caggcactgc ctatgaagag tatgagtatc ctctatcaaa
    54001 gaaaaagctg gaacatgaaa cactgattaa aacctatgac ctcattaagc ctactcgctg
    54061 tctggtggaa gaaaaacagc gatatttgaa aatccagcag gaagacgaaa ccgcctatgt
    54121 agcttacttt accatcaaca gtattgtcgg cgaactggac ttcccgtcct ctgaaatctt
    54181 ctactaccag caacagcaat ttacctttcc gattgatacg tcaatgaatg tggaaattgt
    54241 agcgaatcgt aaagccctat ctactgtccg caataaaaag aaagaactga aagacttgga
    54301 taaccacgct tggcaaagtg ataatgaaac cagctctaat gtggcggaag ctctggaaag
    54361 tgtgaacgag ctggaaacca atttagacca aagcaaggaa tctatgtata agctgtccta
    54421 tgttgtaagg gtatcagcaa atgaccttga cgaacttaaa cgtcgttgta atgaagtgaa
    54481 agatttctat gatgatttga gcgtgaaact ggtacgacct tttggggata tgctgggctt
    54541 acatgaagaa tttttacctg ccagcaaaag atatatgaat gactatattc aatacgtgac
    54601 ctctgatttc ctcgctggtt taggttttgg tgctactcaa atgctgggtg aaaatgaggg
    54661 gatttatgtt ggctacagct tagatactgg acgcaatgtc tatctgaaac ctgctcttgc
    54721 cagtcaaggg gttaagggtt cagtaaccaa tgcgttagcg tctgcctttg tcggttcgct
    54781 gggtggtggt aaatcctttg cgaataacct tatcgtctat tatgcagtgc tttatggggc
    54841 acaagcagtg attgtagacc caaaagcaga acgtggcaga tggaaagaaa ccttgccaga
    54901 gatttcccat gaaatcaata tcgtcaatct gacttctgat gagaaaaaca aaggcttact
    54961 tgacccttat gtaattatga aaaataccaa agattctgaa tcactggcta ttgatatttt
    55021 gacattcctt acggggattt cctctcgtga tggggaacgc ttcccaatcc ttagaaaagc
    55081 cattcgtgca gtaaccaata gtgaagtgcg agggttgatg aaagtgattg aagaattacg
    55141 ggttgagaat acgccactaa gtaccagcat agccgaccat atcgaaagtt ttacagacta
    55201 tgactttgcc catctgcttt ttagtgatgg ttatgtagag cagtctatca gccttgaaaa
    55261 acaactgaac attatacagg ttgccgactt ggtacttcct gacaaggaaa cttcctttga
    55321 ggaatatacc acaatggagt tactatcggt agcaatgctg attgtcatta gtacctttgc
    55381 gttggacttt atccatacag accgaagtat tttcaagatt gtagacttag acgaagcatg
    55441 gagcttctta caggtggcac aagggaaaac actatctatg aagctggttc gtgctggtcg
    55501 tgctatgaac gctggggtat atttcgtgac ccaaaataca gatgacctct tagatgaaaa
    55561 actgaaaaat aacctcggct taaaatttgc ctttcgttcc actgacctta acgagattaa
    55621 aaaaaccctt gccttttttg gtgtagaccc agaggacgaa aacaatcaga agcgattgcg
    55681 tgatttggaa aacgggcaat gccttatcag tgatttatat ggtcgtgtcg gtgtgataca
    55741 gttccaccct gtatttgaag aactgctcca tgcctttgat accagaccac ctgtgcgaaa
    55801 agaggtgtaa atgtgaaacc atcaatatta aacagaataa aatcaaactg gacgctgaaa
    55861 cgtctaggta aagtggcaat gacagtggct ttcacacttg tgattgccat ttttctttta
    55921 gccatgctgg gaacggtggt tcaagctgcg ggcttggtag atgatacggt caatgtggca
    55981 aatgaataca gccgataccc acttgaaaac tatcaactgg atttttatgt ggataatagc
    56041 tggggctggc taccgtggaa ctggtcggac ggaattggaa aacaagtcat gtatggacta
    56101 tatgccatta ccaattttat ttggacaatc agtctttatg tttccaatgc gacgggttac
    56161 ttagtacagg aagcctattc cttagacttc atttccgcta cagcagattc cattggcaag
    56221 aatatgcaga cccttgcagg ggttacgcct agtggactat caacagaggg tttctatgtt
    56281 ggattccttt tactcttgat tttggttctt ggggtttatg ttgcctatac aggactgata
    56341 aagagagaaa ccacaaaggc aattcatgcc ctcatgaatt ttgtgctggt ttttatccta
    56401 tcggcttcct ttattgccta cgctcccgac tacattaaaa aaatcaatga cttttcatca
    56461 gacatcagca acgctagttt gtcactgggt acaaaaattg tcatgcccca ttcagatagt
    56521 caaggcaagg acagtgtgga cttgatacgt gacagcctat tttccataca ggttcagcaa
    56581 ccgtggctac tgcttcaata taacagttca gacattgaaa gtattggtat tgaccgtgtg
    56641 gaaagcctgc tctcaaccag tccagattcc aacaatgggg aagacagaga aaaaattgtt
    56701 gcggaagaaa ttgaagatag aagcaatacc aatatgacga ttacaaagac gattaaccgt
    56761 ttaggtacag tcttcttcct atttgtcttc aatattggga tttccatatt tgtattccta
    56821 ttaacaggaa tcatgatttt ctcgcaggta ctttttatca tctatgctat gtttctgcct
    56881 gtgagcttta ttttaagcat gattccatca tttgatggta tgtcaaaacg agccataaca
    56941 aagctcttta ataccatttt gacacgagct ggaatcacat tgattattac gacagcattt
    57001 agtatttcaa ccatgctcta taccttatcg gctggttatc cgttcttttt gattgctttt
    57061 ctacagattg tgacctttgc aggaatctac tttaagctgg gcgatttaat gagtatgttt
    57121 tctctacaga gtaacgattc tcaaagtgtg ggaagtcgta tgatgagaaa acctcgtatg
    57181 cttatgcacg ctcacatgca ccgtctacag cggaaacttg gacgctccgt gactgcttta
    57241 ggggctgggt ctgccattgc cagtgctacg ggtaaaaaag gacagtcggg ttcgggaagt
    57301 tctgcgagta cacaagcaga tcactctcga ccaaacgggc aggaaaaatc aacactagga
    57361 aagcgtatcg gtcaaaccat cggtacagta gctgatacca aagacagaat ggtagatact
    57421 gctggtaatt tgaaagaaca ggttaaggat ttgccgacca atgcaagata tgcagtatat
    57481 caaggaaaat ctaaagtgac ggataatgtc catgatttaa ccagaagtat ttctcaaacc
    57541 aaagcggata aagccagtgg acgcaagaaa cagcaggaac aaagacggaa aaccattgcg
    57601 gagcgtcgct ctgaaatgga acaggtcaaa cagaaaaaac agccagcttc ttccgttcat
    57661 gaaagaccag ccactaagca agaacaatct catgatggac agacttcaag gcaaacaact
    57721 gtacaggctt cctataggga atctcaacaa gccaaacaag agcgtccaac agttaagtcc
    57781 gattcttcaa gtttaaaaac ggaacgtcaa agtaatacag ttcaagaaag aacggttcaa
    57841 aagccagtaa cttcaactgc accaacagat agagcttcac aacgttcaat cacaaaagaa
    57901 cgtcagtcta ctgttcaaag agtaccacta caaaatacaa aaagtagacc accaatcaaa
    57961 accgccacca ttaagaaagg cagtaagaaa ccatgaagct gaaaacttta gtgattggtg
    58021 gttcgggatt attcttgatg gtcttctcac tgcttctgtt tgtcgccatt ttattttcag
    58081 atgaacaaga tggcggtttt tccaacattc actatggcgg tgtggatgtt tccgcagaag
    58141 tgctggctca taagcctatg gtagaaaaag ttgccaaaga atatggaatt gaagaatacg
    58201 tcaatatact tcttgcgatt atacaggtgg aatcgggtgg tacagcagaa gatgttatgc
    58261 agtcctcgga atccctcggt cttccaccta attcattgag tacagaagaa tccattaagc
    58321 aaggtgtgaa gtatttcagt gagttattag ccagtagcga aaggcttggt gtagatttag
    58381 aatcggttat ccagtcctac aattatggtg gtggtttctt aggatatgtg gctaatcgtg
    58441 ggaataaata tacctttgaa ctggctcaaa gtttctcaaa agagtattcg ggtgacgaaa
    58501 aagtgtctta ccccaatccc atagccatac ctatcaatgg gggctggcga tacaattacg
    58561 ggaatatgtt ttatgtgcaa ctggtaacgc agtatcttgt cacaacagag tttgatgatg
    58621 atacagtaca agccatcatg gacgaagctc tgaaatttga aggctggaag tatgtctatg
    58681 gcggagcttc cccgactacc tcttttgatt gtagcggact gacacaatgg acgtatggaa
    58741 aagctggaat caacttacca cgaacagcac aacagcaata tgatgtaacc cggcatatcc
    58801 cattattgga agcaaaagcc ggcgatttgg ttttctttca ttctacctat aacgctggct
    58861 cttatattac tcatatcggg atttatttag gcgataaccg tatgtttcat gcaggcgacc
    58921 ctatcggtta tgccgactta acaagctctt actggcaaca gcatttagtg ggagcaggac
    58981 gaatcaaaca ataagaaagg acgattcaat gatgaaattc ggaaaaaatc agaatacaga
    59041 aaaacagaca ccaaaggaaa agaaacctcg tgtctataag gtcaatcctc gtaaaaaggt
    59101 tgtgattgtc ctatgggtac ttttaggact tagtttcagc tttgcgatat tcaagcactt
    59161 tacagccatt gatacccata ccattcatga aaccaccatc atagaaaagg aatatgtcga
    59221 tactcatcat gtggaaaatt ttgtagagaa ctttgcgaaa gtctactatt catgggaact
    59281 aaacgacaag tccattgata atcgcataga aaacctgaaa gcatatctga cagaggaact
    59341 tcaagctctg aatgtcgata cagttcgtaa agatattcct gtatcgtctt ctgtaaaagg
    59401 atttcagata tggacggtag aagcagatgg cgacaatcaa tttgatgtaa cctacagtgt
    59461 agaccaactc attacagagg gggaaaatac aaagaccgtc cactctgctt atatagtgag
    59521 tgtctatgta gacagtactg gaaatatggt cctcattaag aatccgacca ttactagcat
    59581 accaaagaaa tcagactata aaccaaaagc tattgaaagt gagggtacgg ttgattccat
    59641 tacaaccaat gaaatcaatg agtttttaac gacgttcttc aagctctatc ctacagcgac
    59701 agccagtgaa ctttcctact atgtgaatga cgggatatta aaaccaatcg gaaaagagta
    59761 catctttcaa gaactggtaa atcctattta caatcgtaag gataatcaag tcacggtatc
    59821 gctgacagtg gagtatatcg accagcagac caaagcaacg caggtatctc aatttgattt
    59881 ggtacttgaa aagaacggga gtaattggaa gattatagaa taacaaatat tggtacatta
    59941 ttacagctat tttgtaatca cgtactctct ttgataaaaa attggagatt cctttacaaa
    60001 tatgctctta cgtgctatta tttaagtatc tatttaaaag gagttaataa atatgcggca
    60061 aggtattctt aaataaactg tcaatttgat agtgggaaca aataattgga tgtccttttt
    60121 taggagggct tagttttttg cacccagttt aagaatacct ttatcatgtg attctaaagt
    60181 atccggagaa tatctgtatg ctttgtatgc ctatggttat gcataaaaat cccagtgata
    60241 agagtattta tcactgggat ttttatgccc ttttgggctt ttgaatggag gaaaatcaca
    60301 tgaaaattat taatattgga gttttagctc atgttgatgc gggaaaaact accttaacag
    60361 aaagcttatt atataacagt ggagcgatta cagaattagg aagcgtggac aaaggtacaa
    60421 cgaggacgga taatacgctt ttagaacgtc agagaggaat tacaattcag acaggaataa
    60481 cctcttttca gtgggaaaat acgaaggtga acatcataga cacgccagga catatggatt
    60541 tcttagcaga agtatatcgt tcattatcag ttttagatgg ggcaattcta ctgatttctg
    60601 caaaagatgg cgtacaagca caaactcgta tattatttca tgcacttagg aaaatgggga
    60661 ttcccacaat cttttttatc aataagattg accaaaatgg aattgattta tcaacggttt
    60721 atcaggatat taaagagaaa ctttctgccg aaattgtaat caaacagaag gtagaactgt
    60781 atcctaatat gtgtgtgacg aactttaccg aatctgaaca atgggatacg gtaatagagg
    60841 gaaacgatga ccttttagag aaatatatgt ccggtaaatc attagaagca ttggaactcg
    60901 aacaagagga aagcataaga tttcagaatt gctccttgta ccctgtttat catggaagcg
    60961 caaaaagcaa catagggatt gagcagctta tagaagtgat aacgaataaa ttttattcat
    61021 caacatacag aaagaagtct gaactttgcg gaaatgtctt caaaattgaa tattcggaag
    61081 aaagacaacg tcttgcatat gtacgccttt atggcggaat cctgcatttg cgggattcgg
    61141 ttagaatatc ggaaaaggaa aaaataaaaa ttacagaaat gtatacttca ataaatggtg
    61201 aattatgtaa aattgataag gcttattccg gggaaattgt tattttgcaa aatgagtttt
    61261 tgaagctaaa tagtgttctt ggagatacaa agctattgcc acagagagag agaattgaaa
    61321 atccgctccc tctgctgcaa acaactgttg aaccgagcaa acctcaacaa agggaaatgt
    61381 tacttgatgc acttttagaa atctccgaca gtgacccgct tctacaatat tatgtggatt
    61441 ctacgacaca tgaaattata ctttctttct tagggaaagt acaaatggaa gtgattagtg
    61501 cactgttgca agaaaagtat catgtggaga tagaactaaa agagcctaca gtcatttata
    61561 tggagagacc gttaaaaaat gcagaatata ccattcacat cgaagtgccg ccaaatcctt
    61621 tctgggcttc cattggttta tctgtatcac cgcttccgtt gggaagtgga atgcagtatg
    61681 agagctcggt ttctcttgga tacttaaatc aatcatttca aaatgcagtt atggaaggga
    61741 tacgctatgg ttgcgaacaa ggattatatg gttggaatgt gacggattgt aaaatctgtt
    61801 ttaagtatgg cttatactat agccctgtta gtaccccagc agattttcgg atgcttgctc
    61861 ctattgtatt ggaacaagtc ttaaaaaaag ctggaacaga attgttagag ccatatctta
    61921 gttttaaaat ttatgcgcca caggaatatc tttcacgag
//
LOCUS       DACDYJ010000006        59109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDYJ010000006 DACDYJ010000000
VERSION     DACDYJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.006"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(59..187)
                     /locus_tag="IWM51_000392"
     CDS             complement(59..187)
                     /locus_tag="IWM51_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9969838.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IWM51_000393"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IWM51_000393"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9969839.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="IWM51_000394"
     CDS             complement(1674..2729)
                     /locus_tag="IWM51_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9969840.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IWM51_000395"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IWM51_000395"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP9969841.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IWM51_000396"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IWM51_000396"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP9969842.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IWM51_000397"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IWM51_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP9969843.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IWM51_000398"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IWM51_000398"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP9969844.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IWM51_000399"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IWM51_000399"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP9969845.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="IWM51_000400"
     CDS             10491..12050
                     /locus_tag="IWM51_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP9969846.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="IWM51_000401"
     CDS             complement(12161..14092)
                     /locus_tag="IWM51_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9969847.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IWM51_000402"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IWM51_000402"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9969848.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="IWM51_000403"
     CDS             complement(15021..15773)
                     /locus_tag="IWM51_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9969849.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="IWM51_000404"
     CDS             complement(15932..16141)
                     /locus_tag="IWM51_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969850.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="IWM51_000405"
     CDS             complement(16152..17480)
                     /locus_tag="IWM51_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969851.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(17845..18432)
                     /locus_tag="IWM51_000406"
     CDS             complement(17845..18432)
                     /locus_tag="IWM51_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP9969852.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IWM51_000407"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IWM51_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP9969853.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="IWM51_000408"
     CDS             complement(20012..21301)
                     /locus_tag="IWM51_000408"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP9969854.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="IWM51_000409"
     CDS             complement(21455..22387)
                     /locus_tag="IWM51_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969855.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="IWM51_000410"
     CDS             complement(22544..23203)
                     /locus_tag="IWM51_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9969856.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="IWM51_000411"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="IWM51_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9969857.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="IWM51_000412"
     CDS             complement(24244..24534)
                     /locus_tag="IWM51_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969858.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="IWM51_000413"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="IWM51_000413"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP9969859.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /locus_tag="IWM51_000414"
     CDS             complement(26821..28176)
                     /locus_tag="IWM51_000414"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP9969860.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="IWM51_000415"
     CDS             complement(28200..29369)
                     /locus_tag="IWM51_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969861.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /locus_tag="IWM51_000416"
     CDS             complement(29366..30253)
                     /locus_tag="IWM51_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP9969862.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="IWM51_000417"
     CDS             complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="IWM51_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP9969863.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34320..34841
                     /locus_tag="IWM51_000418"
     CDS             34320..34841
                     /locus_tag="IWM51_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9969864.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35540..36105)
                     /locus_tag="IWM51_000419"
                     /pseudo
     CDS             complement(35540..36105)
                     /locus_tag="IWM51_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36284..36940)
                     /locus_tag="IWM51_000420"
     CDS             complement(36284..36940)
                     /locus_tag="IWM51_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969865.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="IWM51_000421"
     CDS             complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="IWM51_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP9969866.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38477..39475)
                     /locus_tag="IWM51_000422"
     CDS             complement(38477..39475)
                     /locus_tag="IWM51_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP9969867.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="IWM51_000423"
     CDS             complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="IWM51_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP9969868.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40491..41222)
                     /locus_tag="IWM51_000424"
     CDS             complement(40491..41222)
                     /locus_tag="IWM51_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP9969869.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41226..42491)
                     /locus_tag="IWM51_000425"
     CDS             complement(41226..42491)
                     /locus_tag="IWM51_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP9969870.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="IWM51_000426"
     CDS             complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="IWM51_000426"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP9969871.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="IWM51_000427"
     CDS             complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="IWM51_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP9969872.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47833..49335
                     /locus_tag="IWM51_000428"
     CDS             47833..49335
                     /locus_tag="IWM51_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9969873.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49337..50074
                     /locus_tag="IWM51_000429"
     CDS             49337..50074
                     /locus_tag="IWM51_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9969874.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="IWM51_000430"
     CDS             complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="IWM51_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP9969875.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="IWM51_000431"
     CDS             complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="IWM51_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP9969876.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="IWM51_000432"
     CDS             complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="IWM51_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP9969877.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="IWM51_000433"
     CDS             complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="IWM51_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP9969878.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPIILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(55574..56740)
                     /locus_tag="IWM51_000434"
     CDS             complement(55574..56740)
                     /locus_tag="IWM51_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP9969879.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(56756..56983)
                     /locus_tag="IWM51_000435"
     CDS             complement(56756..56983)
                     /locus_tag="IWM51_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP9969880.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(57370..57626)
                     /locus_tag="IWM51_000436"
                     /pseudo
     CDS             complement(57370..57626)
                     /locus_tag="IWM51_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            complement(57969..58434)
                     /locus_tag="IWM51_000437"
                     /pseudo
     CDS             complement(57969..58434)
                     /locus_tag="IWM51_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
BASE COUNT        16267 a        12824 c        10302 g        19716 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    30541 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    30601 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
    30661 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
    30721 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    30781 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
    30841 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
    30901 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
    30961 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    31021 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    31081 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    31141 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    31201 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    31261 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
    31321 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
    31381 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    31441 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    31501 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    31561 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    31621 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    31681 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    31741 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    31801 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    31861 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    31921 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    31981 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    32041 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    32101 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    32161 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    32221 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    32281 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat
    32341 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    32401 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    32461 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    32521 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
    32581 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    32641 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    32701 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    32761 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    32821 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    32881 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
    32941 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    33001 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    33061 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    33121 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    33181 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    33241 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    33301 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    33361 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    33421 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    33481 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    33541 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    33601 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
    33661 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    33721 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    33781 tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    33841 cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg caccagtttt
    33901 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    33961 tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta attcagccat
    34021 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    34081 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    34141 atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt tcttttattt
    34201 ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga caaacagtct
    34261 aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca
    34321 tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt
    34381 catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg tattgccgac
    34441 aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc
    34501 gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac gtgatgcacc
    34561 ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa atattcgata
    34621 ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac
    34681 taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc
    34741 aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt gatgaatcta
    34801 tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag gagcgtagga
    34861 caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg gaagcagaag
    34921 caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa
    34981 atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa gctgagcaaa
    35041 ttatctccac caaataattt attcatattt ttgacttgcg catactaact aattcttaaa
    35101 ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg gtgattcaca
    35161 ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc
    35221 ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg tttattttaa
    35281 acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa
    35341 atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact cctaactcct
    35401 ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca cagcctagta
    35461 tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa
    35521 atggattaat agatttcgat tattcaaagc gattttctat tggtggattg tgttcccaac
    35581 taacaagacg gattccaaag tcattatata actcttcaag agttaaccct ttcctgttcc
    35641 caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg ccaagacctg
    35701 gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa atccagctcc
    35761 tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata attgttgtgc
    35821 cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc tacctgcatc
    35881 tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc
    35941 tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc ttggtggctt
    36001 aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac
    36061 aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc gcaaatcacc
    36121 tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc tactgatcgt
    36181 aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta
    36241 cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt
    36301 taacaatcgg ttcagatatt tcccaattca ctagccttat attatattga tttaacaatt
    36361 cttcttcatt tattgtattt tgatcaagcg gaccaaattg ttctaataat tctttgtcat
    36421 gtaaaatatc ccttctagta atgcctcttt ccctttcaat gaaagaagca ctaggaaaag
    36481 tatgattata accattcaac ctatctccca tgtaataatt agcaggtcct ggaaaataaa
    36541 atatttgctt attatcacta acttctaaca gcaacactaa atcttctccg ctggctaaac
    36601 gacttgtcca tctacgatat ctccctatgc cttcgttcat tccaaatcca tcaaattcaa
    36661 ctattaacgt gtcttctatc acatgtaatt cttcattcaa ttcatactca agtctaaaag
    36721 gaaattctcc atatgtaatc tctggcaatg gtggtgctgg agttctccaa ataaatacaa
    36781 taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct gctcgaagta
    36841 aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa ataaaaagta
    36901 ctgcaatcat aatgatagta aaagttgtaa aaaaacgcat ttttgccctc cctttataaa
    36961 catcgattct caaattctaa accaaatctt ggtagttcaa gttcaggtat aacgtattcc
    37021 aaccatacat ctcaattact atttatgaac ttatgattat cactgaataa atcactccat
    37081 ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc taatccaatg
    37141 aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt aagaagagca
    37201 gttctcaagg atgatcgagc agttccttcc agttttttaa ttaatcaggc cagttataga
    37261 taaatacttg atcttatcta tatcaggact tctatattaa gcaaaaattt atgtacaaaa
    37321 ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa aacaatagaa
    37381 gcctttccaa attatgttac tccagtaaag atggaattat agtttgacgg aagaaaagca
    37441 agtataactt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta tcagtttttt
    37501 ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt ttgcgaattc
    37561 ggtgattaat tcccgatttt gatatcgcac ctgagggaat catttcccct aattctttca
    37621 agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt tcaggaaggg
    37681 catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta gaagccgcat
    37741 caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc gaatttctca
    37801 tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca ccaatcaatg
    37861 ttaagaaatc agcaattttt tctgctcctt ttaaataaga aatatagcca ttgcgccgtt
    37921 caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct tggttatgct
    37981 gttcataaat agaaaatatt tccaaatgat aacggctcgt ttccggatta ttgactgaac
    38041 ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt cccataatct
    38101 cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc atcaacacat
    38161 cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat ttcatttttt
    38221 tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa tagatacgtc
    38281 tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc acaataccta
    38341 aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct cgatgaactt
    38401 ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc tcctcctttc
    38461 tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat tcttctacta
    38521 ctttgtctcc atcgtggaag acaccaccat ctttcaattc caaaaagtcg gtggaaatca
    38581 ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact agatactcat
    38641 catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc aatactgtat
    38701 cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca tctgtgaaat
    38761 gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca gcagttgtct
    38821 tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg gtgaataaac
    38881 ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact tttcgagccg
    38941 ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg gttttacggt
    39001 cgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaagaca gcatgcaagg
    39061 tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt tttgctaata
    39121 attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg atcagattac
    39181 cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg aaaatatctt
    39241 catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca ccaggaggag
    39301 ccatagcgat cgattctctg attgcaccgc tgcttccccc atcatctgca acagtcacta
    39361 ctgctgtgat atctgctcct tgatttcgca aacttttcag gataactggc agaccagtgc
    39421 caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt ttcacgaccg
    39481 gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt tatagttttt
    39541 ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac cgcctgtaca
    39601 gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca atacagtttc
    39661 cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca tcacataatc
    39721 atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat gagggttggg
    39781 caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt taaaaccaaa
    39841 tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact cattgatttt
    39901 ttcacgtaat tgtcgtggtg tcaatgtcgt cgtatcaatg atcacggaag cttgggcttt
    39961 caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac cgtccatcgc
    40021 cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt ctgaagcatc
    40081 taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca gcatgttttg
    40141 gatttcttca aagaaggaac gagaacgtaa atccactcct aaagccattt ttgtgacttt
    40201 gcctgattct ttgattaatt cccagaattt tggaatcaat cgaggcggca tattatcgat
    40261 acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg caccgctcat
    40321 acctgttatg atgactaatt gcaaattatc tgtcataatt acgaactcct ctcacattgt
    40381 gcatagtaaa attatatcat tccgggcgta tcattaccag ttttcttttt cttttacaaa
    40441 aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt ttacttttcc
    40501 gatgctgtat ccaatgcctt gcttctttca gctagcgctc gttcaatcgt ccgatctgct
    40561 aaaatagatt gggcatcgat cactggataa tggttatctt cagcaaattc atgcatcaat
    40621 gacaattctg tgcaacctaa gataactttt tcacagttta ggcgctctac cgcttcttct
    40681 aagatttcgt aatatagctc ttgattcaaa taatccgatt ccttgatctc atgatagatc
    40741 agataattga ttttttcttg aagaacagta tcaggaatca ctgtttcgaa tccaagattt
    40801 ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag aatcgctact
    40861 ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat atgaaggata
    40921 ggaatatccg ttgccgcttg taattcttca aaaaagtagt gtgcggtgtt acaagtcaac
    40981 acaatgaagt tcggctgcaa taaattttgt ttctcaatat catccagcag aaacggcatt
    41041 ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt tgcatgattg
    41101 aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg gttgatcaag
    41161 cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa aaaattctcc
    41221 atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata gttatggaac
    41281 atatatttca tcaacaacca gcgcttgatt gaccggtctt tttcataaaa cacagtagta
    41341 ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt ttcccgagca
    41401 tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc tgattgattc
    41461 gttccatagg tcgtttcaac aaagggtttg ttaaacactc gatcttctgt gacgaaacga
    41521 gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct caaattgatt
    41581 tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt ggcaaagcct
    41641 gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc gttataatct
    41701 ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa cagtggatga
    41761 ccaagacaca tcatccgaac ttgatgatct tcatctacat acgcatttag cacacgcata
    41821 ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata accggcttcg
    41881 tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa agcttttttt
    41941 cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa agcaactggg
    42001 aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc tctgacgatc
    42061 aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct tactttattg
    42121 atcagtcttt caaataaaga ataatcaata tatggacaga taaaatattc tgacagctct
    42181 tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa aagatatttt
    42241 ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat aaacacaggg
    42301 tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg ggtaggggct
    42361 aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa agaacgtgcc
    42421 ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc attttcaata
    42481 ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc aactacacaa
    42541 ggaatatcat accataaaga aaaaagcaat caaaggaaac tcctccgatt gctcaattct
    42601 tttttgaacg gattgaactt ataggaagtt tttgtctata ccaatcagtg tagaaagaac
    42661 taccatgtaa tgattctaga acactgatat taataaaaca agcaaaaatt cattttttta
    42721 tgttttttta actatctcag ctatctatcc tctttatacc attatttttt tcaaatagtg
    42781 acctgtataa ctttctttta cgttaatgat ttcttctggt gttccagtcg caaggatcgt
    42841 tcctccacct tctccacctt ctggtcctaa atcaatgata taatctgctg ttttgattac
    42901 atctagattg tgttcaatca ctaaaactgt gttaccagca tctactaatc tttgtaatac
    42961 atgcaacaag cgggcgatgt catcgctatg aagtcctgtc gttggttcat ctagtatata
    43021 gaaattcttt ccattagaga ttttgtgcaa ttcactggca agtttcatcc gttgtgcctc
    43081 accaccggac aatgtcgttg ctggttgccc catcgtcaca taacctaagc caacatcaac
    43141 aatcgtttgc agtttgcgat gaatctttgg aatgtgcttg aagaattcta cagcatcttc
    43201 tactgtcatt tccaaaatat cagcaatgct ttttcctttg taatgcactt ctaatgtttc
    43261 agagttatat cgtttgccat gacatacttc acaaggaaca tagacatcag gcaaaaagtg
    43321 catttcgatc ttaataattc catccccgcg acaagcttca caacgaccgc cttttacgtt
    43381 gaaactaaaa cgccctttct tataaccccg catttttgcc tcgttcgttt gagcaaataa
    43441 cccacggata tcatcaaata cacttgtata agtcgctgga ttacttctcg gcgtccggcc
    43501 gattgggctt tgatcgatat cgatgatctt ttcgatactt tcgtagccgg aaattgtctt
    43561 gaatttacct ggcttagcag aattcttatt taatttttgc gctaacgatt tctttaagat
    43621 actattgacc aatgtactct ttccagaacc agacacacct gtgactgcta caaattctcc
    43681 taatggaaat tcaacactaa tatttttcag attgttttct gaagcaccag tgatcttgat
    43741 tgctttccca ttacctttac ggcgttcttt tggtacaggg attgattttt tccctgaaag
    43801 atatttccct gtcaaagaat tttcatcatt agctacttct tcaggagtgc ctgcagccac
    43861 gatttgtccg ccttgttctc cggccccagg tccgacgtca atcagataat ctgctgccca
    43921 catcgtatct tcatcatgtt cgactacaac gagggtgttg ccaagatcac gcattttctt
    43981 taatgagcca atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg gttcatctaa
    44041 aatatacaaa actccagaaa gattcgatcc aatttgtgta gccaatctga ttcgctgcgc
    44101 ttctccccca gataatgttc cggaagcgcg actcaaagtt agatagtcta gtcccacatt
    44161 ttccaagaat gaaagtctgt catttacttc ttttaaaatc ggtttggcaa ttactttctc
    44221 ttgttcagat aacgttaagt ttgagaagaa atctactgct ttttgaatag acaactcatt
    44281 tgtttgcccg atattcgttc cattgatttg aacagataga gcttggggat tcaatcgata
    44341 acctttacat gtctgacaag ttaattctgt catataaaga cgcatttgct cacgcgtgaa
    44401 atcactgttc gtatcatgat aacgacgctc gatattcttc aatacccctt cgaaagtcgt
    44461 ttctacatcg cgaacgccac caaaatcatt ttcataatgg aaatggaaaa gatcatctgt
    44521 cccattcaag atgatatcct gatgctcttc cggtaattct tcgaatggtg tatctaaatc
    44581 gataccgaaa ctttcacaag cctgcgctaa catttgagga taatactgag agctgattgg
    44641 attccatgga acgatagcac cttgactaag tgtcttcgtt ctatctggaa tcactaaatc
    44701 ttgatcgact tctaatttga cacctaaacc atcacagtct ggacaagctc caaaaggcgc
    44761 attgaatgag aacagtcttg gttctagttc gccaacagta aatccgcaat aaggacaggc
    44821 atagtgttcg ctaaacaaca tttcttcttc accgatcacg tcaaccagag catagccgtc
    44881 tgccaatcgc agagccgcct ctaaagaatc aaatagacga gaacgaatcc cttctttcac
    44941 aatgatccgg tcgatgacga tcgcgatatc atgttttttg tttttctcca gctcaggcac
    45001 ttcacttaaa tcatagatct caccatcgac tcgaacgcgg acgtatccct ctttttggat
    45061 tcgttcgaat atttttttat gttgtccttt tttctttacc acgatcggtg ccaacacttg
    45121 gatctttgtg cgctcaggca aagccattac ttgatctgcc atttgatcta ctgattgact
    45181 ggtaatctcg atatgatcgt tgggacaaat cggatgtcct acacgtgcaa acaacaagcg
    45241 taaatagtca ttgatttccg tcacagtacc tactgtcgac cgaggatttt tacttgttgt
    45301 tttctgatca atagaaatcg ccggactcaa tccgtcgatg ctatctacgt ccggcttatc
    45361 catttgtcct aaaaactgac gagcataagc agaaaggctc tctacgtatc tccgctgccc
    45421 ttcagcgtac agcgtatcaa atgctaggga actttttcca gaaccggaca aacctgtaac
    45481 gaccacgaat ttatctcgtg gaatcgttac atctatattt ttcaagttat gggcacgtgc
    45541 accgtgaatc gtaattttat cgttagccat gttgtcctcc tactttgaag cttttaattc
    45601 taaaatcata tctcgcacat tagcggctgt ttcaaaatcc aatgctttcg ccgcattttt
    45661 catttctttt tctaatttca tcaataacgt atctttttct tctttcgtca tttcttcata
    45721 cgagacttgt accgcttctt ttgtatcatc ttccgattct ttcgtaatag aaatcaaatc
    45781 tcggatttct ttaatgatcg tttttggaac gatcccatgt tcttcattgt acttctgctg
    45841 aatcgtacgg cgtcttgatg tttcatccat cgctagacgc atagaatctg ttactttgtc
    45901 tgcgtacatg attactttac cttcttcatt ccgagcagct cgtccaatcg tttggactaa
    45961 ggaacgttca ctacgtaaaa agccttcttt atccgcatcc aagatagcaa caagggaaac
    46021 ttcaggtacg tctaatcctt cacgaagcaa attgattcca actaacacat caaattcacc
    46081 taatcgaaga tctcgaatga tttctgttcg ttccagcgtt ttgatatcac tatgcaaata
    46141 tttgactttg atccctaatt ctttgaaata atctgtgagg tcttctgcca tttttttcgt
    46201 caatgtcgtt acaaacaccc gctggtcttt ttcgactcgt tcatttattt cgcctactag
    46261 atcatcgatt tgtcccatga ttggacgaac ttcgatcact gggtcaagca gccctgtcgg
    46321 acggatgatt tgttggataa cggtatctgt ttgttcatgt tcatatggtc ctggcgtcgc
    46381 agagacataa atgatctgat tgacatgttt ttcaaactct tctaaccgaa gtggccgatt
    46441 gtctaaagca gatggcaaac ggaaaccgta atctactagc atctgttttc tcgctcggtc
    46501 accattatac attcctctga cttgaggcat ggttacgtgc gattcgtccg ccacgatcaa
    46561 aaaatcttca ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt tacgtccgtc
    46621 catatgccga gaataatttt caattcccga cgtgtagccc atttctaaca tcatttcaat
    46681 atcgtaattg gttcgctgtt ctaaacgttg agcttccaga agtttgtttt catttcgcaa
    46741 gacagttaaa cgctgttcta attccgcttt gatattcgct acggcatgct ccatatgttc
    46801 atcattcgtc acaaagtgag tcgccgggaa gattgccacg tgttccgttt ctcctaaaac
    46861 ttcacctgtc agtgcattga cttctcggat acgttcgacc tcatctccaa aaaactccac
    46921 acgtaaggca cgttcatctc ttgaagccgg aaaaatctcc acgacatcac cgcgcactcg
    46981 aaaacgaccg cgctgaaaat ctatatcatt tctttcgaat tggatactga ctaagtcacg
    47041 aatcaactga ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt gtttttggta
    47101 ctcaaaaggc gaacctaatc caaaaataca tgaaacggat gcaatgacga tcacatcatt
    47161 tctttctaat aatgaactag tagccgaatg tcgtaatttg tcgatctcat catttacact
    47221 tgaatctttt tcgatatacg tatcgcttga aggcacataa gcttcaggct gataataatc
    47281 ataataagaa acaaaatatt caacagcatt gttagggaaa aattccttga attccccgta
    47341 cagctgacca gccaatgttt tgttatgggc aataattagt gttggtttat tcactttctc
    47401 aataagattg gaaatcgtat atgttttccc tgttccagtc gcacctaata agatttgtgc
    47461 ttttttcccg ccgactacgc catccactaa ttggttaatc gcttcaggct gatcaccagc
    47521 cggttggtat ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa tcattcttcg
    47581 tcctcctttt cattcattcc ttttcatttt acgaccaata aaaagaaaag gaaagcaaag
    47641 gcattcctta ctaaaaaatt ataccacacc gaacatactt tcgccacttt tttatcttat
    47701 tatgcatgtt acgttttata acataaaatg ttagaaactc gcattttttt cattttttcg
    47761 agcttacagt cttgtctgat tcagcacttt tcgcctataa taataaaata ttggaaattt
    47821 aggaggttga ttatgaaaag aaagcattta tttcactcat ttgttttctt aatgacagga
    47881 cttgtgacac ttgcgtttgg acatgttgca caagctgaca acactacaac ggcagagtca
    47941 agcacaagcg agacaatgca aaaaatagag cccaaaaaag acgtatacgt aattgccagt
    48001 gactctgctt ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga aggcatcgat
    48061 gtaaaattga tggaaaacat cgccaaactg caaggattta acatccgaat ggactaccgt
    48121 ggtttctctg ctgctttgca agctttggaa tcaggacaag cagacggaat gattgccgga
    48181 atgactgtaa cagatgaacg aaagaaaagc tatgattttt ctgattctta ctttgatagc
    48241 gggatccaat tagctgtcaa aaaaggagat acttcgatca agtcttatga agatttgaaa
    48301 ggaaaaacag tcggagcaaa agttggtacc gaaagtgctg aatttttaag cgaccatgaa
    48361 tctgaatatg gctattctat caaatatttt gatgctgctg atcaattgta cgacgggtta
    48421 aaagttggcg caatcgatgc aatgatggat gattatccgg taatcggtta tgcgatcaaa
    48481 caaggacaag aattggaaac accgatcaaa aaagaatctg gcggaaaata tgcatttgcc
    48541 gttaaaaaag gacaaaatcc tgagcttctt aatatgttca atgaaggatt gaaagaatta
    48601 aaaagaacag gcgaatacga taagattatt aaagaatatg tagcggatgg ttcagaaact
    48661 tccagtgagt cttccactgc tgatgaatca acatttcttg gacttatcca aaacaactat
    48721 caagcattgt tgaaaggact ttggagaaca attctcctaa ctttgatctc ctttgctttg
    48781 gctatagtaa tcggtgtgat ttttggatta ttcagtgtag caccaatacg cgctcttcgc
    48841 acaattgcta gtatttatgt cgatatcatc cgaggaattc caatgatggt tcttgcgttc
    48901 tttattttct ttgggttgcc tggtatcgtt ggatttacga ttcctgactt ccttgctggt
    48961 gttatcacct tgactcttaa cgcaagtgct tatatcgcag agattgttcg aggcgggatc
    49021 aacgccgttc ctgtcggaca aatggaagct tccagaagtt taggtctttc ttataatcgg
    49081 acaatgcaaa aaatcatttt gccgcaagcg atacgcatca tgatcccatc attcgtcaat
    49141 cagttcgtta tctcattaaa agacacaacg atcatttccg caatcggtgt agtcgaattg
    49201 ctgcaaactg gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt ttacttgatc
    49261 attgcgatca tgtacttgat tttgatcaca gcattgacaa aattagcaaa agttttagaa
    49321 aagaaggtga agtaagatgg cagaaaaaat cttagttgag catttagtca aaaaatacgg
    49381 cgataacaca gtattaaacg acatcaatgt gtccatcaac gaaggagatg tcgtctgtat
    49441 cattggccct tcaggttcag gaaaaagtac atttttgcgt tgcttgaatc gtttagaaga
    49501 accttctgac ggtgaaatta ttattgacgg cgcccacttg atggataaaa gcacagatat
    49561 caacttggtg agacaacata tcggaatggt attccagcac tttaacctat tccctcatct
    49621 ttctattttg gaaaacatca ttttagcacc gatggacctg aagaaagaat ctagagaaga
    49681 agcagagaaa aaagcaatca aattgctgca gacagttggt ctagaagata aaaaagatat
    49741 gtatcctgaa atgctttccg gtggacaaaa gcagcgtgtg gctattgctc gtgctctagc
    49801 gatgaaccct gacatcatgc tttttgatga gcctacatct gcactcgacc ccgaaatggt
    49861 cggtgatgtc ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga tggttatcgt
    49921 tacgcacgaa atgggcttcg ctaaagaagt ggccaatcgt gtcatgttca ttgacgacgg
    49981 aaacttttta gaagacggaa cgcctcaaca aatctttgaa aatccgcaaa atgaacggac
    50041 aaaagatttc ttgaataaag tattaaatat ttaataaaaa ccaaaaatgc ccggtcgctt
    50101 catgacgacc gggcattttt agtttcttgt tttttcactt aactgtacca gccgcatcgc
    50161 ttgttctcct gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat
    50221 cggttcattg acgatatcaa gtacatagtc gataccacct tcagaaacta tgatgattat
    50281 cttattctaa agctggcttt tttgttggta caaacatgac aactacaaaa aaaccaggcg
    50341 aacgtatgat tgttcgtctg gtttttaatt attaatgact tagcgttttt actagaaata
    50401 gaaaatttgg gcaagtctac tttatatttt tactagaaaa actacttccg ctttttcccg
    50461 ccaaaagcat ctttaaactt atcgaagaaa ccgtcttctt gctgttcttc aactttgatg
    50521 ccgccagctt cagcgaatgc acgtaatgct tctctttgtt gttcatttag atttttcggt
    50581 gtgatcagtt ttactttgac atgttggtcg cctgttgcac caccacgtaa cttcggagcg
    50641 ccttttccac gtaaacggaa gttcgtacct gtctgtgttc cagcagggat cttcaatttc
    50701 acatctccat ggacagttgg tacttggact tcatccccca aagctgcttg tacaaaactt
    50761 aaaggcaatt catagtaaat ttcagaacct tcacgatcaa agatatcact ttcttctaca
    50821 cgaaaaacca catacaaatc accaaatggt ccgccgtttt ctccagcttc tccttgacca
    50881 gctaaacgca tttgttgtcc ttcttctaca ccagctggta cattcacttt aactgaatgt
    50941 gctttcttct catgtcctga gccatgacag gttggacaag gttctttgat ttctttccct
    51001 gtgccatgac aaacatcaca agtttgacgg ctcatcactc gtccaagtgg cgtttggcgt
    51061 tctacattga tcgtacctgc accatgacat ttatgacaag tagttggttg tgtgcctggt
    51121 tttgcaccgt ttccaccaca agtatgacag atttcttccc gattatactt gatttctttt
    51181 tctacaccaa aaatcgcttc ttcaaaagac aagtcgaccg tatattgcaa atcagcacct
    51241 tgacgaggcg catttggatc gactgaccga ccgcctcctc caaagaaaga gtcgaagata
    51301 tcttcaaaac caccgaagcc gccaccagag aagccgccga atccaccaaa tccgccacca
    51361 gctccaccag caccgtaatt tggatctgtg cctgcatgcc cgtattggtc gtatgcagcg
    51421 cgtttttgcg ggtcacttaa aatctcatat gcttctgata cttctttgaa tttttcttcc
    51481 gcatcaggtt ctttattgat gtcaggatgg tattttttcg aaagcttacg ataagctttc
    51541 tttatttcat cttctgatgc tccttttgag agccctaaga cttcataata atcacgtttt
    51601 gttgccatag gctttcctcc tattggttct catcattttc acgtcgcttg ttatcatatc
    51661 atattttgtt tgaaacaaaa accgatactt caaactattt ttcaaaagaa aaagccaaag
    51721 tagcaacttt ggccttctct cgaaattcag tgaactgatt atttatcgtc gccatcgact
    51781 tcttcaaaat cagcatcaac gacatcatct gctccgcctt gagctgcttc tggattttct
    51841 tgagcttgtt gttgtgcagc ttgttcgtat agtttaacag tcaagttttg aacgatttcg
    51901 ttcaatgcat cgcgtttagt tttcatttcc tcgatgttgt ttgcttcaac agcggctttt
    51961 aattcgtcac gagctgtttc tgcttttttc acttcttctt cgtccacttt gccttctaat
    52021 tctttcaatg ttttgtccac agagaatagc aatgcgtcta catcattacg taggtcaact
    52081 tcctctttac gagctttatc tgcttcagcg tttgcttctg catctttcac catacgttcg
    52141 atttcttcgt ctgttagacc tgaagaagat ttgatcgtga ttttttgttc tttttgtgta
    52201 cctagatctt tcgcagatac atttacaatc ccgtttttat caatatcgaa tgttacttcg
    52261 atttgtggaa taccacgagg agctgctgga atatctgtca attggaatct tcctagagtt
    52321 ttgttgtcag cagccattgg gcgttcacct tgtaatacat ggatgtctac tgctggttga
    52381 ttatctgccg cagttgagaa tacttgtgat ttacttgttg gaatcgttgt attgcggtca
    52441 atcaatttag tgaacactcc acccattgtt tcgataccta atgacaatgg tgtaacgtca
    52501 agtaaaacaa catctttcac atcaccagtg atcacgccac cttggatagc agcacccata
    52561 gcaactactt catcagggtt aactgatttg tttggttctt ttcctgtttc tttacgtact
    52621 gcttctacaa ctgcagggat acgagtagat ccaccgacta aaataacttc gtcgatttct
    52681 gattgtgaca gacctgcatc ttttaacgct tgacgtactg gtacttttgt acgttctact
    52741 aagtcagctg ttaattcatc aaattttgca cgtgtcaatg tcatttccaa atgcaatggt
    52801 ccagattctc ctgctgtgat aaatggcaag ctgatttgtg tgcttgatac gccagataaa
    52861 tcttttttcg ctttttcagc agcgtctttc aagcgttgta aagccatttt gtcttgagac
    52921 aagtcaatgc tgttttcttt tttgaattct gctaccatgt agtcgatgat tttgttatca
    52981 aagtcgtccc cacctagatg gttgtcgcca gctgttgaca atacatcaaa gacaccgtct
    53041 cctagttcaa ggatagatac gtcaaaagta ccgccaccaa ggtcgaatac taagattttt
    53101 tcgtcgcgat ctgttttatc taaaccatag gcaagagcag ctgcagttgg ttcattgacg
    53161 atacgttcta cttctagacc agcgatctta ccagcatctt ttgttgcttg acgttgtgca
    53221 tcattaaagt aagctggcac agtgataact gctttttcga ctttttcacc taaatagtct
    53281 tctgcgaatc ctttgatata ttgaaggatc attgctgaga tttcttgtgg tgtatatgat
    53341 ttaccttcta catctacttt gtaacctgct tcaccgatat ggcgtttgat tgatgaaatc
    53401 gtatttgggt ttgtcacagc ttgacgtttt gcaacttccc ctacttggat ttctccattt
    53461 ttgaatgata caacagaagg tgttgtacgg tttccttctg ggttagcgat gatttttgct
    53521 tcgtttcctt ccaatactgc tactgcagag ttagtcgtac ctaagtcaat accgataatt
    53581 ttactcataa ttaacgtctc cttctttaat tgaaagtgta agttttatat ccgcacaatt
    53641 attgtgcaac aatgaccata gttggtctta acacgcgatc atgtaatttg tagccttctt
    53701 gtagtacttc tacaatcgta tcggctggtg tatcttcagt cgctggaaca gtttgtactg
    53761 cttgatgaag attaggatca aaggcttctc ctttagcagg gattttttca attccttctt
    53821 cttccagtgc gttacgcaaa ctttccaaga ccatttccac gccttttttc aaacctgccc
    53881 cttgatcatc tgacacttct gtggctaatg cacgttccag gttgtcaatt gatggaagta
    53941 attttttggc taaatcttgt gaacggtatt tttgtaattg ctcacgctca ttttttcctc
    54001 gatttgccat atttgcgatt tcagcacgag cccgcaagta tttatcttcc atttcttcta
    54061 actcagcttt taatttcgct gtttcgaatt cttccgcttc agcttctgaa atacccgaat
    54121 cttctgcagc ttcgacatcg atttccggtt ctggctgagc atctgtcatt tctttatcta
    54181 gttcttcttg attttctttc acgatacagt cttcctttca ttattcagtc agctgtttga
    54241 ttgctcttac taagaaagag agaacgatca gctgcaaata gcttcgttat tcacattacg
    54301 agtttgacaa gtcaagcgag cggtagtaat cagcaagttt agctgctagt tctcttcgat
    54361 aaacatcgac tagaccgaac atccgagaat aaggcatact agtaggccct aacaaggcta
    54421 ttgtacctct tccatgacca tctatctcgt aacttgcttg aatcatactc atattctgca
    54481 ataaatcatt gcctaattca gcaccaatct tgatatgaat attgctatcc atcggagtga
    54541 tcatttgtgt aagtccgtct gagtctttca taaaagaaaa catcgatttg aactgatcaa
    54601 gatcttgacg gggctcataa tccaagagat tcatttgtcc actgacataa accttttctt
    54661 caaaaacatg accaagaacg gtgtcaaata actgagacat cccttctgtc gtctggaaat
    54721 atttatgaag aatgatcgga atctccgtac gaagtttatg gtacaccgtc atcagaggat
    54781 ctccgactag acggtcgttt acaatctgta ccattttttc tagttcctga cttcccatat
    54841 attcgggaag agtgaacact tggctttcta cattccccat atctgttacg acgatagcaa
    54901 taatctgtcg attattcaag ggtacgatac gaaagccagt cagtcggcgg tcttttattt
    54961 caggtcccaa tgaaaaagtc gtatagcttg tcagctcaga taatatctca gcagattgct
    55021 taataatctc attgatttca tgaaattctc tgcctagaga ctgacgaatc agcagcaaat
    55081 catcttctgc tacgcgagca ggttttaaca aatgatccac gtaataacga tagcctaaag
    55141 cagatggaat ccgcccagaa gaagagtgag tcttcaacag caatccttca tcttctaatg
    55201 ctttcatatc attgcgaatc gttgctgagc tggccttgac tccctcagac attaatgttt
    55261 tggaaccaac gggcacgcca ctactcgtat agtgttggat gatcagacgt aaaatgtcgt
    55321 tttgtcgctt tgttaacatc ttcctcacct ctcattagca ctcatatatt ctaagtgcta
    55381 attcacttat aaatatagca agaagccctt ctcctgtcaa gagaaagact ccatttttta
    55441 gcactctaaa cacaagagtg ctaattctaa ataattgatg taaatccagt tatatcaata
    55501 cttcaatacc tcattgtcaa actttctaaa ctttttcttt tcaagtttcg ataaaaaaca
    55561 aatattcttc aaactatttt tcaaataaaa agcgctcaaa cacattgttc cccaaaaaag
    55621 tccctcttgg tgtcaaataa acacggtctt cttcattaac aagtaaccct tcttgctcta
    55681 attcccttag cacatcaccg tacaccgatt caattgtttt ttgatagcga tcatagaaat
    55741 gttttttagt aatccctgtt ttttttctta gtcctaagaa catttcttct tctatttgat
    55801 tttttcgtgt caatttttct tcttccaata ccgggcgttc atttgctcgt aatgggcgta
    55861 aatagtgttg gattggtcct ttgttgcggt aacgaatatc tccaagataa ccgctagcac
    55921 cagctccaaa cccaaaatag tggtcattgt tccagtacat gaggttatgt tgagattcat
    55981 gtccggagag cccaaagtta cttatttcat attggtgacg tccatgcttt tccattgctt
    56041 caatcgtctc ttcaaacatt ctggtttccg tttcctgatc aggcagttcg agtcttcctt
    56101 gtcgtaccca gttcatgaac attgttttgt tttcgaggat caatgagtac aaagaatagt
    56161 gaggcagatc caaagccaaa gctttttcca aagtatcccg atatccttct agtgtttgtc
    56221 ccggtaatgc ataaatcaaa tcgatgctta cattggtaaa attttctgct tctaaaaatt
    56281 gcattgtttg atacacatct tctgcagtat gtttacgtcc aatttttttc aataagcgat
    56341 tgtcaaatgt ctgcactccc atggataaac gattgacgcc atagtttttc atcacttgaa
    56401 gtttttcacg ggtcaaatca ccaggattcg cttctacagt aaactcatta ccattttgaa
    56461 aaggcaggat ctctctagca cctgataata gtcgatcaag ttgtttagca gataaagaag
    56521 ttggtgttcc gcctcctaca taaagtgttt ccaaattcgc tgtaggatga ctttccagca
    56581 ttaatctcat ttcattcagc agcatttcca catactcatc tactggctgc ccttctaaaa
    56641 aaactttatt aaagtcacag taatagcaga tatgctcgca aaatgggata tgaatgtacg
    56701 cagaaacctt gttttctgcg ggatttattg tttctatcat tgaaattctt tctccttacg
    56761 tcgaaaagtc gtgcctgttt tcgaccttct taaaatcgcg ttcaagcacc aactcattga
    56821 aataagccgc atatttagaa tcaaggaaac ctagctctaa aaatgcttgc ttattgtcat
    56881 cggagctaca cggtgctttt acagcctctc gataaacggt aatcgcaaag ctgaccttcg
    56941 gcattaagtc aaaatctgca aaaatatgaa aagctatttt cacagattcc tccttaagtg
    57001 ctcagaccac aacgctttgc ttacaacctc tcatatacgc atactaaacg tatttggcga
    57061 acaaatccac taaaaaatcc aagaatcgac ttcttggatg atttttttac actcatgatt
    57121 cttcctaaac aattttgtcc ttattttatt cataaaaaaa tctagtcaaa tctctcactt
    57181 caagagtttt aactagattc caattattat ttttttgtaa aaataaacag tctcttaatc
    57241 atagacattt cttcggtgcc ctacttgaat agcgataaca acgaattgat tatcttctat
    57301 ctctaccaat acacgtattt gccaatcaga gctgcttctg aatttcgggc gtgaatttta
    57361 aacggcaggt cattccagcc caccaaattc attaaggatt cttccatgaa tactgttgtt
    57421 ttcccacttc ttatccaatg tttatgggca atttcagccg tttgacgatt gtattcatct
    57481 tctagttgtt ccatcgaata gttttttaga agttcagata aagtaatccc ataaaattca
    57541 gccatataag aaagccattc ttttttggcg tcacttacac gaaccgtaat catagcccta
    57601 taattgcctc tcttaaagaa tctcatgcat ttcctgatgt gattttagaa aaatttactt
    57661 gctaaaaaac tgtgaaaatg taggagctag catccttgta gtaaatgatc gagctcttta
    57721 ttatacatta aaaaagttca gaaattttga tgaagtagcc aactactttt tcatgagtaa
    57781 agcaacacta gaaaaagaat cctccagtac ttagaattta ctggaggatg tacttttgaa
    57841 gaagcctata ggttatttaa aaaatattac tacaggaagg aacgagaatt acttcgttac
    57901 ttataataga acttagtctt atatagaagg catagctcac attagcttat ttgtcctata
    57961 aaaataaatt acggtatttt agaaaagcta ttttctcttt tttcaacgat cttcagcaat
    58021 ttttctttat cgtcaggtaa ctcaagttca tgaattctag catgacaatt gggacataac
    58081 gcgatacagt tttcaatcac atcttttcca ccttgcgaca aatattctat atgatgacaa
    58141 tgtaaataag gtacaccgtc ttttataaat ggcccttttt cttggcaaag ttgacaaata
    58201 ccttttgcaa gtcgtttaac atattcagat acgaagggat tacgtttata tatttttgtt
    58261 tttgagaagc aaaactcctc ttcgttaaca tattttgcag ccattttttt attataatta
    58321 ctaacttttc ttgcttcttc ctctaattta gcattagata aattacgaat atgtctagtt
    58381 tgattttctt cctggttata tatctttatc ttcaatcaat tgctcttgat ttattaaagc
    58441 taatggaaat ttatatactt ttctgctgtt gcccgaacta tctttttcat taacagtata
    58501 tatttgtcct gctaaatata cttctcccag atatgtgtgt tcttttggtt taaatgtttc
    58561 aaataaatga atctttacaa aattactttt tgaattggct aaaactttat tttgattctt
    58621 atccaatact tgatcccctt ttagccccat accggtatat tgtaaaatac catttaacca
    58681 cttatcttta taaacattac ttcctgtttg atcactaaac agtacaagag tattagtttg
    58741 atgagatctt ctcattccac cctgtttgga acatttaaac ttctctgaaa tttctttgtt
    58801 agtatatact ctaccaactt ctaactcacc taatatgttt ttagaccaat tttctcgcca
    58861 tgtaataatc ttatcaagat taaacatttc tccctctttt ggcatgccat ttctaattaa
    58921 attagctgga atggcaacag tttttttgac aaattttata aggtgcagaa cttctttccg
    58981 tatgctattc cgattgtcct tgacaatgag cctcctcgga tgtgacgatc ttcggcagga
    59041 gctaacagtt aaagttagac tgcctcgcta gcgttagcac atccgagctc attatcaagg
    59101 attcgctgc
//
LOCUS       DACDYJ010000007        58837 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDYJ010000007 DACDYJ010000000
VERSION     DACDYJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.007"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(159..1469)
                     /locus_tag="IWM51_000438"
     CDS             complement(159..1469)
                     /locus_tag="IWM51_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9969881.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="IWM51_000439"
     CDS             complement(1481..2899)
                     /locus_tag="IWM51_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9969882.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="IWM51_000440"
     CDS             complement(3139..4707)
                     /locus_tag="IWM51_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP9969883.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="IWM51_000441"
     CDS             complement(4711..5121)
                     /locus_tag="IWM51_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9969884.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="IWM51_000442"
     CDS             complement(5118..6041)
                     /locus_tag="IWM51_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP9969885.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="IWM51_000443"
     CDS             complement(6038..6916)
                     /locus_tag="IWM51_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9969886.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="IWM51_000444"
     CDS             complement(6903..8240)
                     /locus_tag="IWM51_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9969887.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="IWM51_000445"
     CDS             complement(8243..9346)
                     /locus_tag="IWM51_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9969888.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="IWM51_000446"
     CDS             complement(9420..10928)
                     /locus_tag="IWM51_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9969889.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="IWM51_000447"
     CDS             complement(10894..11586)
                     /locus_tag="IWM51_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9969890.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="IWM51_000448"
     CDS             complement(11608..12582)
                     /locus_tag="IWM51_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9969891.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="IWM51_000449"
     CDS             12882..13754
                     /locus_tag="IWM51_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9969892.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14212..16410)
                     /locus_tag="IWM51_000450"
     CDS             complement(14212..16410)
                     /locus_tag="IWM51_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9969893.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16642..17634
                     /locus_tag="IWM51_000451"
     CDS             16642..17634
                     /locus_tag="IWM51_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9969894.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17694..19115
                     /locus_tag="IWM51_000452"
     CDS             17694..19115
                     /locus_tag="IWM51_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP9969895.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19108..20607
                     /locus_tag="IWM51_000453"
     CDS             19108..20607
                     /locus_tag="IWM51_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP9969896.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20646..22040
                     /gene="uxaC"
                     /locus_tag="IWM51_000454"
     CDS             20646..22040
                     /gene="uxaC"
                     /locus_tag="IWM51_000454"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9969897.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22199..22882
                     /locus_tag="IWM51_000455"
     CDS             22199..22882
                     /locus_tag="IWM51_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP9969898.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22884..23720
                     /locus_tag="IWM51_000456"
     CDS             22884..23720
                     /locus_tag="IWM51_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9969899.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23731..25485
                     /locus_tag="IWM51_000457"
     CDS             23731..25485
                     /locus_tag="IWM51_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9969900.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25761..26102)
                     /locus_tag="IWM51_000458"
     CDS             complement(25761..26102)
                     /locus_tag="IWM51_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9969901.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26170..28341)
                     /locus_tag="IWM51_000459"
     CDS             complement(26170..28341)
                     /locus_tag="IWM51_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9969902.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28552..29409
                     /locus_tag="IWM51_000460"
     CDS             28552..29409
                     /locus_tag="IWM51_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9969903.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29480..30337)
                     /locus_tag="IWM51_000461"
     CDS             complement(29480..30337)
                     /locus_tag="IWM51_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9969904.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30322..33024)
                     /locus_tag="IWM51_000462"
     CDS             complement(30322..33024)
                     /locus_tag="IWM51_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9969905.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33037..34326)
                     /locus_tag="IWM51_000463"
     CDS             complement(33037..34326)
                     /locus_tag="IWM51_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP9969906.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34476..35522
                     /locus_tag="IWM51_000464"
     CDS             34476..35522
                     /locus_tag="IWM51_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9969907.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35524..37677
                     /locus_tag="IWM51_000465"
     CDS             35524..37677
                     /locus_tag="IWM51_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9969908.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38150..39601)
                     /locus_tag="IWM51_000466"
     CDS             complement(38150..39601)
                     /locus_tag="IWM51_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9969909.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39598..41322)
                     /locus_tag="IWM51_000467"
     CDS             complement(39598..41322)
                     /locus_tag="IWM51_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9969910.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41315..41929)
                     /locus_tag="IWM51_000468"
     CDS             complement(41315..41929)
                     /locus_tag="IWM51_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9969911.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42274..43731)
                     /locus_tag="IWM51_000469"
     CDS             complement(42274..43731)
                     /locus_tag="IWM51_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9969912.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(43772..44692)
                     /locus_tag="IWM51_000470"
     CDS             complement(43772..44692)
                     /locus_tag="IWM51_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9969913.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(44704..45651)
                     /locus_tag="IWM51_000471"
     CDS             complement(44704..45651)
                     /locus_tag="IWM51_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9969914.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46130..46849)
                     /locus_tag="IWM51_000472"
     CDS             complement(46130..46849)
                     /locus_tag="IWM51_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9969915.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(46898..48544)
                     /locus_tag="IWM51_000473"
     CDS             complement(46898..48544)
                     /locus_tag="IWM51_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9969916.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            48723..49313
                     /locus_tag="IWM51_000474"
     CDS             48723..49313
                     /locus_tag="IWM51_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9969917.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49405..50910
                     /locus_tag="IWM51_000475"
     CDS             49405..50910
                     /locus_tag="IWM51_000475"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP9969918.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(50994..52346)
                     /gene="celB"
                     /locus_tag="IWM51_000476"
     CDS             complement(50994..52346)
                     /gene="celB"
                     /locus_tag="IWM51_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9969919.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52346..52864)
                     /locus_tag="IWM51_000477"
     CDS             complement(52346..52864)
                     /locus_tag="IWM51_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969920.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(52880..53206)
                     /locus_tag="IWM51_000478"
     CDS             complement(52880..53206)
                     /locus_tag="IWM51_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP9969921.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53219..55198)
                     /locus_tag="IWM51_000479"
     CDS             complement(53219..55198)
                     /locus_tag="IWM51_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9969922.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55219..55533)
                     /locus_tag="IWM51_000480"
     CDS             complement(55219..55533)
                     /locus_tag="IWM51_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP9969923.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            55701..55994
                     /locus_tag="IWM51_000481"
     CDS             55701..55994
                     /locus_tag="IWM51_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP9969924.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56072..57184)
                     /locus_tag="IWM51_000482"
     CDS             complement(56072..57184)
                     /locus_tag="IWM51_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9969925.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            57571..57750
                     /locus_tag="IWM51_000483"
     CDS             57571..57750
                     /locus_tag="IWM51_000483"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969926.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            57944..58114
                     /locus_tag="IWM51_000484"
     CDS             57944..58114
                     /locus_tag="IWM51_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9969927.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(58538..>58837)
                     /locus_tag="IWM51_000485"
     CDS             complement(58538..>58837)
                     /locus_tag="IWM51_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9969928.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        17655 a        11988 c        10283 g        18911 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt
    13861 atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca
    13921 gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata
    13981 agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag
    14041 cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt
    14101 tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt
    14161 ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata
    14221 tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt
    14281 ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt
    14341 caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc
    14401 tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat
    14461 cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga
    14521 caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa
    14581 ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata
    14641 aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac
    14701 gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc
    14761 aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat
    14821 ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc
    14881 agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata
    14941 cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt
    15001 actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg
    15061 gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt
    15121 ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc
    15181 cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc
    15241 taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa
    15301 ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac
    15361 aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc
    15421 tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt
    15481 tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa
    15541 taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat
    15601 tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca
    15661 attattataa tctgatttca gtactatata cgttttatct ttttctctta tcactttcag
    15721 ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt
    15781 ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc
    15841 accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc
    15901 tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa
    15961 taaattattt ttgttattta tgtacttctg ttgatacttt tcaatttcct cttcggtcag
    16021 ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt
    16081 attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt
    16141 ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc
    16201 atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg
    16261 cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg
    16321 gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa
    16381 tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag
    16441 gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa
    16501 gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat
    16561 caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga
    16621 aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg tcgctaaagc agctggcgtt
    16681 tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa
    16741 caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt
    16801 ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact
    16861 tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac
    16921 agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac
    16981 gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa
    17041 gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac
    17101 gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga
    17161 catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa
    17221 aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta
    17281 acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct
    17341 gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct
    17401 gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct
    17461 ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg
    17521 tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct
    17581 aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca
    17641 agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa
    17701 aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg
    17761 aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat
    17821 tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga
    17881 accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg
    17941 atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg
    18001 cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg
    18061 caggaatcca attcgtggag actgataatt taacagatac tcccccacag agcctcccgg
    18121 gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca
    18181 ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg
    18241 cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct
    18301 gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca
    18361 atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg
    18421 tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg
    18481 tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac
    18541 cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa
    18601 tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta
    18661 tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct
    18721 tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca
    18781 gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt
    18841 taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac
    18901 atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta
    18961 ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg
    19021 tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat
    19081 tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta
    19141 aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg
    19201 attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg
    19261 ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg
    19321 acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt
    19381 gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga
    19441 acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta
    19501 gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa
    19561 aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt
    19621 tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat
    19681 cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa
    19741 ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat
    19801 gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa
    19861 aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt
    19921 tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg
    19981 attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa
    20041 gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat
    20101 gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt
    20161 aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca
    20221 ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt
    20281 agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat
    20341 tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg
    20401 gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat
    20461 gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc
    20521 atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc
    20581 atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa
    20641 gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc
    20701 attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa
    20761 tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca
    20821 aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg
    20881 attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt
    20941 acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag
    21001 aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc
    21061 gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag
    21121 acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt
    21181 cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac
    21241 tactggaaga agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag
    21301 ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca
    21361 tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag
    21421 ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt
    21481 tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta
    21541 actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg
    21601 atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt
    21661 tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga
    21721 tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca
    21781 acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg
    21841 ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat
    21901 atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg
    21961 aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt
    22021 actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt
    22081 gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt
    22141 cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat
    22201 gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc
    22261 cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa
    22321 ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct
    22381 agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa
    22441 aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt
    22501 ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg
    22561 agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat
    22621 gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga
    22681 attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga
    22741 tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc
    22801 tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc ctcttgctaa
    22861 ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt
    22921 tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata
    22981 tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg
    23041 ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag
    23101 gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt
    23161 tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg
    23221 gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca
    23281 gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg
    23341 aagcagggat caatcatcca atctataact atcagttagc taaacgttta atcgatgaag
    23401 tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc
    23461 atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag
    23521 cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca
    23581 agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt
    23641 acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt
    23701 ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa
    23761 aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt
    23821 tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc
    23881 gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt
    23941 tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt
    24001 catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt
    24061 atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc
    24121 cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc
    24181 agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt
    24241 cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt
    24301 ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct
    24361 gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa
    24421 cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta
    24481 gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg
    24541 atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc
    24601 gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg
    24661 attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct
    24721 ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt
    24781 tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct
    24841 ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct
    24901 acactcctta acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat
    24961 gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct
    25021 caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac
    25081 gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac
    25141 cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc
    25201 caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta
    25261 gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat
    25321 tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag
    25381 gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa
    25441 ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct
    25501 atgacaagac ttttgtcgta gctcctattt ctgaataaac ggtgttctcc tgccaactcc
    25561 tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa ttgttccttg
    25621 taactcagag ttaaacggta ggctcacgac ctcttggtaa atggtgttca agagacagcc
    25681 tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt tcacaccctc
    25741 tgttgtttca taaaatagtt ttattcgctg taaaggtcgc gaactggtgt catgatgata
    25801 cggttcaagt cattgatgat tgtactgaag ccttgctctt tttgcatcaa gtcattgatt
    25861 acttcttctg attggatttt cacagccatt tcttgtgctt tcattgcatc ttcgtcgctg
    25921 aactcttgtc cactcatttg tttttcttgt aattcgattt gcatcgcttg gaagtctttg
    25981 aacagtttgt gtgcttcttc gtttgcttgt actttctcgt acgcttcttt caacgctttg
    26041 aattctggta attcacggat ctcacgttcg atttgattcg cgctatcgta gatgtttgac
    26101 atgttatgcc ctcttttctt ttagtagtta ctctctattg taacatgggg ttattcgttt
    26161 tcaaaggggc tactctcctt gatatttccg ccagaggtct ttagccgctt caccaattcc
    26221 ctgaacgcct tctttcacca tatcgccgaa gtttttcagc ccttcttttg cttgttcacc
    26281 atattgattc aagttatctt gccaatttcc agactctcca ttagaatttc ctgtgtttcc
    26341 aacttgatct gcagctacca cttgtcctcc tgtggcatag gcatctgcta cactaaatcc
    26401 tgtttttgaa gtatacggaa ggatgccttc tgccgctgat ttgaagactt tcccgacaac
    26461 atttccactt gtcccttcca aatagtgcag cttgctggat tcttcgtaac ccatccatgt
    26521 cgaaatcact acgtctggcg tgtaaccgat catccattgg tcatttgctt ttgtggcatc
    26581 gaaattagtt tctgtggttc ctgttttccc tgctatcgta taacctgcag gattcgcagc
    26641 agccgctgtt ccatttgaaa atgtaccaag aagcatgctg gtcatttcat cggctgtttc
    26701 ctttgatatg acctgttttg gttttttgtt tgtgttatcg acgatcaccg ctccagtcga
    26761 atcaacgatt ttcgtgataa gatgcggcgt atacatctga ccatcattgg caaatgcact
    26821 gtaagcaccc gccatgatca atggagatac tcctttttcc agtccaccaa gggctaagcc
    26881 ataatactta tcagattttg ctaaaggcag gccgaattct tctgctttat catatccttt
    26941 ttgtaaaccg atctcatgca aaagccatac agtaggcaaa ttcaaacttt gtgcaacagc
    27001 ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat cgtaataact
    27061 ttgcggttcg tctttcaaga tactggaagg cttgtatcca gcttccagtg ctggtgtata
    27121 tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag caaagctgaa
    27181 tcctctaaag acatgatctc ctcttcgtcc tactagtgct tgtacaccac ctgtattcgg
    27241 atctaaagct actgaagcag attggaccat ggcaccatct gaagcatttg ctgggaataa
    27301 tgcactgttt tgataagtag cttctaattg cttttgataa tcctgatcta aagctgtata
    27361 gattttatag cctttattca tgacatcttc ttcatcaaga ccatactcgt taactgcttc
    27421 atcgatcact gcatcaaaat aataaggata acgataacca gaatctgaat tatcgtatgt
    27481 gtcattaaga tacgcgctga tatctgtctg gctttctgct tgttcttgtt cttttgtgat
    27541 ttttccgttt tcttccatta cacttagtac tgtgtttttc cgatcattcg cattttttgg
    27601 gtgatcgatc ggattataaa taccaggacc tttcagcatt cctgccaaag tagcagcctc
    27661 agacagcgta acattcgttg catctactcc aaaatacttt ctggaagcgt cttggacacc
    27721 ccataccccg ttgccaaaat aggcattgtt aagatacatg gtcaagattt cttctttgct
    27781 gtacttcttt tcgatctcga ttgccaaaaa taattccttc gcttttcgat caaacgtttg
    27841 gtctaatgtc agatacgcat ttttggcaag ctgctgagtg atcgtacttc ctccgccccc
    27901 tgatgttccg ccagacaaga ccattcggac tgctgcacgg gcaatccctt tgatatcaaa
    27961 cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt ggatataggg
    28021 agaaatcgcg tttaattcta catatgttcc tttttgggcg tacagtgtgc cagcttcttc
    28081 gtttttctta tcataaatga ctgtcgattg gcttaatcct gattttaacg tttcgacatt
    28141 tgcttgtttt gctaaaataa agaggtagat acttgtcact aagacacaga tcaaaccaat
    28201 cagcaaaatc aatttgttca catgatattt tttccagatg cgctttcgcc atcgatggaa
    28261 ttgtccgaga tatggtctga tccaacgcca aaaactgatc aaagcagctt ttatcttttc
    28321 taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa tcgtcttatt
    28381 ttaccatagt gaaagagtgt tttcttcctg cttaagttgt agttcaatat gtttttttac
    28441 gttagtgcaa gtagagtaac ataagaatga acagaaaaat gtaagttttt tcttaaatat
    28501 gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa ggagtacact tgtgaaaatc
    28561 acgatcactt tacctatgaa ccatcctgaa acaacaattc gcgacttgct tgaaaaggaa
    28621 tggttagttc ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt ttggatcaat
    28681 caacaacctg ctttgttcca tcatgaagta aagggcaatg accagatcac attgttattc
    28741 gaagaaacag attataatta tcaagaagtt caattaggag atgcgaagca catccgtgtt
    28801 ttatatgaag atgagcattt gattatagtg gataagcctg caggaatgaa gactcatcca
    28861 aatgaaccga cagaaaacag taccttactc aatcatttag ctgcttatct cgacactaaa
    28921 cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc cgttttattt
    28981 gcaaaaaatc catttgtctt gccgatactt ggaagaatgt tggaacaaaa aatgatctat
    29041 cggagatatc aagccatcgt atggggagaa attgccaaag atatgaccat caccaataaa
    29101 attggacgag atcgccatga tcggagaaaa agggtcgtag atgataaaaa tggtcagagc
    29161 gccttgacac atgtcactgt caacaaagta ctttctgaac aaacacaagt ctattgtgtt
    29221 ttggaaaccg gacgtacaca ccagatcaga gttcatttag ctcataaggg acatccgatc
    29281 gttggagacc ctctctacca aaatagaaca gctgaacgac tgatgctcca tgcattagaa
    29341 ctgcacatga tccatccatt tactcaagaa aaaatagtcg gtaaagcttt acctggatta
    29401 tgggaataga aataaaaaag aacagagtct tatcagcaga taatgataag actctgttct
    29461 ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc tgctaacagc aaactaccga
    29521 taatcccact ttcattcggc agtccccaac gaacgatata ttcacttact tgaggtgttt
    29581 ccatataacc acccatcaat tcaacaaatt gctgacggat tttttgaagg agatgatcct
    29641 gattcattac cccaccgcct aaaataatct tttcaggtgc taacgtcaac gtatagttga
    29701 tcagtgcttg agcaatataa taagcctgaa tagcccaaac aggatgatct tgcggcagat
    29761 cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat ccttctaaac
    29821 aatctttatg ataaggacaa gtaccttcat aatggtcatc tggatggcgt ttcacccaaa
    29881 tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc acgacacctg
    29941 caccgattcc tgttcccacg gtaaggtaaa tacaactatt ttttccttgt gctgctcctt
    30001 gcttcaattc cccatatgca gcagcattga catccgttgt ccaagcagca ggaatattgt
    30061 agcgttcctt gattgtgcca agaaaatcga attgtgccca cccttttttg ggagttgcca
    30121 aaacgtatcc gtaattatct tttgttttgt cgataccaat cgggccgaat gagccaatcc
    30181 cgattccctt caattcataa cgatcaaaaa attcaaatac ctgatccaat gtttcttctg
    30241 gtgttgtggt agggatactt aatttttcta aaatttctcc accatctgaa accgcacaca
    30301 caaattttgt tccgcctgct tctattccgc catacattcg ttttcctcct cgaatccgat
    30361 tgtccgaatc tcataaggtt ggattattgg gacaaccgct tgcttcgttg gttcttcaag
    30421 cagattcaat atgacagttt ttccattgtc tttagttatt tccaattttt ctaaatgact
    30481 agacatattg aaacctctta ctactacttt atcactaaac ttgctgcgtt tcaaagctgt
    30541 aattgcaaac gtttcacttt ttatcgttaa atattgttgt tttgaaatca atgttccttc
    30601 atgatgtttg atttgttgag tggaaaaagg aatctgtgct gcgtatgcat gcaaataagt
    30661 agagaatttt tcttctggtc catgtagttc aatgctgtaa ttgaaccgat gctcacctag
    30721 acactgtgct tctggtgttg gaaaatatcc ccaatcgcct agttctccca cactgcgaag
    30781 tagcgtcact gcgatttgtc cgtcttctgt tacttcatat tcattcaaac caaagtttcc
    30841 gactgttaca ccgtactctt catcatgaat attgacaaat gcttgctgat gttgcggatt
    30901 cgtggggttt tcccaacttt tagacacatg gtttgggcgc ttcaccactt caaaaatact
    30961 atctgcttcg tgatgttcca catgtaactt tgtaggaaac aagacacgta agcgatgatc
    31021 tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt ttctgattgt
    31081 catgattgtt tttatttgaa ggattcgttt ttctttggat cgttctgctt ttcgataacg
    31141 aaattccaca accatctgct gttctttttc taaacgctcg tctgcagaaa cagggatttc
    31201 catttcctgt agaatactta tttttgctat ttccggtgta tctactaata cttttacttc
    31261 agagttttct ttgttactgg ataggattgg ctgatcacag aaaggttgtt taaagatata
    31321 ctcatttcct atatcgccga tatcttcaaa aatatgcaga ttattcaact ttcgtttgtt
    31381 tttgcgatct tccattgtaa tggtaccatt tttttcaatc gtcaaatgta gagggccgtt
    31441 ttcgatgatt cgtccactct gatgaatcat tgtttccttt tcttctgctt tcgcttcccc
    31501 ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg gtaaagtcac
    31561 tttgatcatt cttgccatat atggcacacg gaaacggtct ttcggcaaat cataaccaaa
    31621 ttggacagtt tcttcagata aaatagctgg tatagtggcc ccactttttg tttctacatg
    31681 atacttacgt ttaggcaaat tttctaactc ttgatatagt tgttcaggta ttccttcagc
    31741 gaatcttttt ctttctaatt cgatggttat ttctgcttca ccagttttat ccattcctgc
    31801 agtatttaca atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat cgattgctgc
    31861 ggtcagctgt tctaaggaat cttgtgcaag atactttcca acttcatctg ctttttcaaa
    31921 acgaggtatc atttcccgat ggacagaatc gactgaacaa ccgcaaatac tgtcgtgagg
    31981 atgattttgc attaatgtct tccaagcata atctaattga tcatggggat agtttccact
    32041 aacttcataa gccatcgtcg caagtggttc tgtaatattt tctagctgtc gctggacctt
    32101 ggtattccat tgttttaagt aaacccgagc agatgctgta ttggctaaag tataccagcc
    32161 atccgtttct tgacttgtca attctccttc tactgagcct aagtcttctg gcacatcttt
    32221 ttgaactgct tccagatagt cagtaaaatt agaatgaata aactcgtaat ctggaaacag
    32281 ttcatttgcg agatggattg cttttgatat atctttttgt accggttgat gatcaacacc
    32341 attcatcatc aataagtggt tcgtagaggc atattgttct gcatccgcta atttttgctt
    32401 ccaaaaggca agagctgctt ctttttctac tggaatctca ttcccgttgc tataccaatt
    32461 ggcaaatagc agaccaaaga ttgctgtttg atcaggtcct ttccaccaca tctctgagta
    32521 ttgggaagaa tagttttctg cttccaaaac ttgattatca aagccaattg gttttacacc
    32581 acggccaaac gctgctgctg aaataccagc ttgtttcatt aattgaggcg tctgtcccat
    32641 atttccaaat gtatcgggaa aatagcctaa catgacaggg gttccccatt tacggctttc
    32701 ttccatgcca atcagcatat ttctcacgtt cgattccgag ctgataagaa aatcatcttg
    32761 caagatataa aatggtccaa ttctcaattt tccttcattg attgcttgtt ggattgcctg
    32821 tcttttttct ggccttactt gaagatagtc atctaaaata atcgtttgcc catccaaatg
    32881 aaaactgttg aaggacggat caacttcaaa tagctctagt agatcgtcta tcaattcaat
    32941 cagtcgcatg tgatgctgtt cgtaagccat gtaccattca cgatcccagt ggctatgaga
    33001 aatgatatat actttctttt tcatttacga tacctcctac ttttctactc taatatcaaa
    33061 gtaatccatg actaattcgc aaaacatcat atttgcccaa gaaaaccatt ctctcgtata
    33121 attttcagga ttgttcacat cgaatccttc gtgcataagg tgtgttcctg cgtcagttgc
    33181 tacaagtaaa ttcaatatcc gctctttttc agatttgtct gacgttgtca ttccttccat
    33241 agctaatgca atcggccaaa cgtatttttc tggcgtatgg gaggatccaa ttccttttgc
    33301 gtactttcct tcatagaaat atggattttc tttactcaac aatgttcttc ttgtttgcag
    33361 ataatcttcc tcttgttcag aacaatatcc caagtaaggt gcggcgatca gattgggcac
    33421 attactgtca tccatgactg tggcgtttcc cattccatct acttcatatg cataaatcgt
    33481 ttgccctttt tgattaaccg ttcgtccgtg attctgaatc ccctcttgga tctctttctt
    33541 caatttctgt gtttgctgta caatttcttt atcatcgagt atcgtttgaa aaatttcttc
    33601 cacataacct aacacgacca ccgcaaacat attggatggt accagatatc cataacggca
    33661 agtatcatcg ctaggacgga aaccagacca tgtcattcct gtaaaagctg tttttgcacc
    33721 tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa atgtataagg
    33781 agagtgttca tgatcttgct ctgttttgaa cacgtccaag atttttttga taccttcaat
    33841 aaaagattga ttgaattgat cagtttttcc tgtatttttg taaagaagat aggccaactg
    33901 tattgggtag cataaagaat caatttcata ttttctttcc cagatccaat cgttcatttt
    33961 tgtatgatct gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat tcgcatatgg
    34021 atcgatattg atataaaaaa attgcttttt gactaacccg ctgatcatgt cagctaattc
    34081 ctcatcttct tttgcaatca ctaaataagg acgaacttgt gcagtagagt ctcttaacca
    34141 catcgctgga atgtctcccg tcaataaaaa ggtggttccg tcattttgct ttttgactgt
    34201 tgtcaacaga gtattggcaa atgctgctgt aaaattcttt gcccagtccg catggatatc
    34261 cccacatttt tgtgtgatat cttccataaa tttctgcacc gattttggta tatctttata
    34321 agccactaat aaaaccttct ttctatttga tatatcttta aagattatac ttcatacagc
    34381 caattatgtc tacgctttca aaaaatatct ttttatttac tttttcatcc attatggtat
    34441 tatttgaata tgatatatca ttaagaggtg tttatatgaa tcaaccattg taccagacaa
    34501 tatttaatga tttgcaaacg gctattttaa ctggtgaatt acctgtagac agccaactac
    34561 caactgaaaa agaactttca cgtacctata aagtaagcag aatcacttcc aaacgtgcat
    34621 tgactgaatt agagcaatta ggacttattt atcgcgttcg cgggaaaggc agtttcgtta
    34681 aatccgcttc tccggaacag attcttgctt ctcctacaaa aacacgacgg attctgtttc
    34741 ttcttccata tgtcgctgat ttgtctgttg gtgattttag caagggattg aaccctgtga
    34801 tgcaagataa acagattgat gtcatgatga cgacgcttga ctttctacaa aataaaacag
    34861 caaaagagct aatcaatgaa tttgacggat tgatttatta tgctcttgat acagaacaac
    34921 atcttgactt actttttgag ctttcattga aagaattccc tgtcgtcata ttagataaaa
    34981 aaatctatga gttacctttt cctaccgttc tttccgataa tttccaaggc ggtgcgcttg
    35041 ctgcctctta tttgatcgaa cagggacatt caaaaatcgc ttatttgttt ggtacgcaga
    35101 ctcatccaca atccgttcga cagcgttacc ttggttacct tgaagcattg gacaaagctg
    35161 gtttgacttt ccacacttca ctggatgaca aaagcgcgac attagatgaa ttgctttctt
    35221 atatcactaa gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc gctatccaga
    35281 ccatgaatct cttgagacag catggttatc atatccctga agatttttca attatcggat
    35341 ttgatgatat ccaagcagca gctcttgttg acccgccact gacaaccatc gcacaagatt
    35401 ttgaacagtt aggtaagctt gcgggagatg ctttggtcac ttggttagag acaaaagatc
    35461 gaccatccga tagtaaattt ccagtttcat tcatcaaaag acaatcaaca aaggagaaat
    35521 aaaatgaata tcggtcaaat agatactaga catgcaacag cgaatcagcc aaatttctct
    35581 aatgggaact gccagcctta tactggcgtt ccttttggga tgaattattt tgcaccgcaa
    35641 actacggatc aaaacggcag ctggtggttt catcctgatg accgcgtttt tcaaggctac
    35701 cgtcttaccc atcaaccaag tccttggatg ggtgatttca gtcatatgct gtttacgccg
    35761 atcaatggaa aactacagga gaacaccctt ttccatgctc aaagttctta ccgcccagaa
    35821 gaaagtatat tttgcccgac tcatctgtcg atctgtcaat tacgttatgg catctgttct
    35881 acattgatac ctagcatgta tggcggaatc ttatctattg attacagcaa aaatgacagc
    35941 ggtttacttt tatcctttcc gggacgccat cagctctttg ttatagatcc ttacacagtg
    36001 gaaggaaaaa tcacacattt tgctggttca gaagatcctg atttttcttt ttattttata
    36061 ttgagatttg aatatccttt aacaattgat gagttagctg tttccaacaa aaatagcgac
    36121 tcgatcccgt tgtattttga acagacaaaa aaacaaacta tccgatttgg gacatctttt
    36181 atcagctcgg aacaagcagc ttataacctg gaactagaac aaaaccaaag caaagagact
    36241 taccttgctc acagtcaaga acaatggcag tcctattttg atcgtataca gctcacacac
    36301 catgatcaac aacaattgcg gacgttttat cataatttat atcgggtgtt tttatttcca
    36361 caaacatttt atgaaattga tcctagtggg caaaaaatcc actatgatac gactagtcga
    36421 tctataaaac ctggtgttct ctatacaaat aatgggtttt gggatacgta taaaacggtt
    36481 tatcctcttt attctttgat tgctgtagaa aaatacgaag aaatgctgga aggttttttg
    36541 aacagctata gagaaagtgg ctatttgcca aaatggctgt ctcctgatga acgtggactt
    36601 atgcctggga cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga tatccgacga
    36661 gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg ctacggttca aagcaaacat
    36721 ccaaattatg gacgccaagg aacagaagat tacttgacat atggctatgt tcctaatcac
    36781 taccacgaat cagtcaatca cacattagac tatgcttata gcgatttttg tattagccaa
    36841 gttgccaagg tactagaaga agaggaaatg acgaagaaat atcagcagca atcattgaac
    36901 tatcaaaata tctttgacta tgagtcagga tttatgcgtt caaaagataa agatggcaag
    36961 ttcagagaac cattttcagc taatcgttgg ggacaagatt atgcagaagg aagtgcttgg
    37021 caaagcagct ttgctgttta tcacgatttt tcaggactga tcggaaaata tggtggcagt
    37081 gagagatata aagaaaaact cattgagctg tgcaatcagc ccccaacgtt tgacccagga
    37141 ggatatggtt ttgagatcca cgaaatgagt gagatggccg cgatagaatt tggacaatta
    37201 gctatttcaa accagccaag ttttcactat ccttacttgt tcagttatat aggacaacct
    37261 gaaatggcac agccactgct taaacagttg atgactcaga ccttcgacga ttctccaatg
    37321 ggttaccctg gagatgagga caatggaagt atggccgctt ggtatatctt caatagtctg
    37381 ggattttatc ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc attaattgac
    37441 aaggctgttt tgcttttgtc aaatggaaag gaattgacga ttgaagcaac gcctaatcgg
    37501 ccgcagcaac aatttatcca taaaatcaaa cggatggaca aagaatatac gaaattatat
    37561 tttacccatc aagatttgct gaaaggcggc acgatctctt accaattagg gatcatccct
    37621 caccctaaga aatatcaagc aaaagatttg cctttttcct tatcaaacta taattaacaa
    37681 gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa aaatagatcc
    37741 tcatattttt cgtttggttg gtcttattac cgcagatgca agcttttgaa caccgtttat
    37801 tgagaggttg tgaaatcagc gttctgacct gagtattaga gagacaaatg gcaaaatagt
    37861 tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga acaccgttta
    37921 gtggagaggt cgtgaaaaag ctgtttagtt tcgagtatta agaaaaattt tgtgaaaatg
    37981 gctttccaca tttttgcata aatttggctt aataccgaga aactggcttt tgaactccgt
    38041 ttatcgagag gtcatgaaaa agctgccttg cctcaagtaa taagaacagc ccaaaattag
    38101 atttctagac aattttttat aagttatttc attacattgg ctttttcttt tatgattctc
    38161 cactgttttc tcggtactct ttaggggatc tgccgacgat tttcttgaat aatttagaga
    38221 aatacgtggt gttgttgtag ccaatctcac ttgctatctc attgatgttt tgattactgt
    38281 gcaaaagcag ttgtttcgct ttttccatac gtaaatggtt caaataagct gaaaaacttt
    38341 ttttcgtttc ttttttgaac agctgtccta gatacatgac atttaaatgc aggttttctg
    38401 acacttcttt gagtgtaagt ggttcttgat aatgctggtg caggattacg attacttttt
    38461 ccacattgtc actgtacttt ttctgtctct gatgcttttt gatgaatgac agaagtagct
    38521 gctgaagttc ttgcaccgtt tttgcctgat ggattttttc gattccttgt aaatattcct
    38581 catcttccag atcaatcaat tcccgttgaa tctccataaa cagtaaaaaa ctggaatatc
    38641 gtatatcttc cggcatcatg gctgctaact gaaactgttc aaaaaaatca gtgatcattt
    38701 tttgagcgac ttccaattgt ttattttgca gcacgcgtct gaaacttgaa aaatcgatgg
    38761 tttgatcttt tgtttctgct tgtactgcgt aaaaaacatg ctcgtcttta tctccataaa
    38821 attgatgcaa ctgtagattg tctttcgctt gttggaatgt ttcagggatt ttgtccaaag
    38881 aatctgtctc ttccccgata ctgatcagcc attcctgatc aggaaagttt tcttgaacaa
    38941 aacaacagaa gccatcaact tcatctgcct ccaacaacaa cgtgatcaat acacggtctc
    39001 cgtaatttcg ttgatagtaa aagatttgtt gccgttcttg caaccatcta ttgataagag
    39061 tatcatgagt tcttgccagt tgaatcaata ctatcctatg ctgcttctca cttatttgtc
    39121 ctattatttc ttcttcactt atttcatcca attcttcatt caaccattga ctgaataaca
    39181 gttcttgata gatttcttgt tgattttttt gttcattttg ctgatctaac cgtgttttga
    39241 ttttcttgag gctttcaacc aattcgaact tattcacggg tttcatcaaa taattcacgg
    39301 cgcctaattg catacctcct ttcaaataat cgaattcttg gtatcctgat aaaatcataa
    39361 attcaaaatt atgacgttct ttttgagccg cttcaataaa ttccagtcca tttatttctg
    39421 gcattgtcac atccgttact actaaatcaa tctgtcgctg ttcaagtaca gctagtcctt
    39481 ctttggcatt ctctgctgtt gctactattt gaaagcctaa actttgccaa tcaattatct
    39541 tttttaaccc attcaaaatc atgtattcat catctaccaa taacacttga tacacgctca
    39601 tccctccttg aatgtaatag aaacggtcag tccacctgtc tcattattct caagcgacat
    39661 ataataactg ctgccgaagc tggcacgtaa acgttcattc acgttttgca gaccgataga
    39721 accgtgaagt tccactttag gatgagacag tttttcttca atctgtttta gccgtttttc
    39781 agtcatccct tttccattat ctttgatgat gatccttacc cgtttgcctt cctgcaagac
    39841 tttgacactt agcgcattat cgaatcgagt aaaatcaata ccgtgtttaa aataattttc
    39901 aactaatggc tggatcacaa atttaggtac ttcgatcttt tccaaatcag gatcgatcat
    39961 aaaatgatag gctactcgat taggataacg catttgatac aaatagacat atttttcgca
    40021 aaagtccagt tcttctttta aagtgatcgt tttctcttga ttcgtattgt ttcgaagcaa
    40081 agcggaaaac gcatacacaa catctgctaa ttcttcactt ccttcgctga ttgcatacat
    40141 tcggatatat tctaatgtat tatataaaaa gtgtggattg atctgtgctt gaagcgcccg
    40201 catatgtgcg tcttgctgtt tgatttctaa tttgtagatg tcttctacaa actgattgat
    40261 gctatccaac atttcgttga taccgattga aagttccttc agttcatatt ccgtgtttgt
    40321 tatgtcgatc cgcgtctcta aattccctgc tgctgtttga tccatggcct gcatgacgat
    40381 agatacttgc tgtgaatatc gtttgaatgt tttataaaga aagataagca gtatattgtc
    40441 taaaataatg ataccaatga tcagcggggc aagatttttt cccgtttcga tttttacact
    40501 ttttttatct agtacagcta atatacgata atcatcagac agttcttggt gttgaacaaa
    40561 attgtttttt tccagatcgt caatcggcaa ttgtgaattt ttcttcagac tctctgtgat
    40621 gatcatttct tgtctttcaa acgtttcttt agctatttta ttatcatgga atgtgtatag
    40681 tcgatttgtc gttccagaaa tcatgtataa agagagtcca ttgaatgacg ttagattttc
    40741 taaaatcata tctagttctt tcttttgaaa accaataaaa atacttcctg actcagctcc
    40801 tccttgtgtg ataggcaact tgatataaaa ggcgtctagt aattttggtg tccccttttt
    40861 tatttcccca cctttgtttt ctctagaaga ttcataatac tccggtagtt gattcaacac
    40921 gatatatatc gcacttagtt cttcataatt tgcgtagaga tctttgattt gatttgggaa
    40981 agaaactaat tcatcggtac tgttttgctg ttcgtataca tacatcagat actgatctat
    41041 gggttttgtt aagtaggtcg tcacattttc gatcttttcg gaattcccta caagctggtt
    41101 tttgatgatc ctactcaagg ttgttttatc attcagcatt ctgcttgtgc tttgaacggc
    41161 atcacttgtc atgttttctg cctgtttttg attttgttcc caagtatgga tactaaaaat
    41221 aacggtagca gtcatcgtta caccgatcat gataaccgcg tatttccgca acagacgatt
    41281 tagtaaggtt ccttttgaaa atagaatccg aaacttattc ataactttcc aactgtttat
    41341 ctaattcttc acgccaatgt gttgtcatcg taccattcca aaccgtcaaa agaccaatga
    41401 caccaaacag gatcaatcct ttgaattgcc aagtcacgcc taaaattata ccgctaccaa
    41461 taattatttt tagaaaagac gaaaagctgg aaaaaaccga aataaaactt agctttagca
    41521 gattaggaac agagatcgta tattcactat ctaaaatcat actgtattga taagtagcaa
    41581 ataggcagag caaggcaaaa agtaaaataa aatcaatagc taaaaaaatg agccctttga
    41641 tttgaacaga taaatataaa ttgtaactca ataaagcagt accagataaa aataaactaa
    41701 atagcagatt gcctcttaaa aaatttcttt tatatatatt ccagcctctt tttattgtga
    41761 tttctttata ctcgaatccg tacaaataaa atagttcatt gactgttttc catgccgcac
    41821 ctaatccaaa gatgatcccg ccggcaaatg tatatatcca gaaatacaag gttagtttta
    41881 taatgaccca gatccggata aataactctt ccaaaacctt ccctaacata tcttctcctc
    41941 caaacattaa gtttatactt tctcattata caaaaaaagg ttgggcaatc agcaccttga
    42001 cctgattatt agagaaacaa acggaaaaat agttcctcat attttgtcgt tttgtgagcg
    42061 aataccgagg gttagctgat tgaacaccgt ttaccaagag tttgtgaaat agccgcttag
    42121 tttcgagtag taagaaaaat tttgtgaata tggctttcca catttttaca tatatttagc
    42181 ttaataccga gaaactggct tttgaacacc gtttattcgg aacaagaggt tgtgacacgt
    42241 cttttgtcac aacccctttg tctttattca ttattattct tgtcggaact catcatattg
    42301 tttttgcatt tctttcaata ccttatcata gccagccttg tccaaagcgt ctttcaattt
    42361 cggtaatgtt tcatctggat caacagtccc tgtattcaat ccatctaaat attgattcat
    42421 gacattagaa atattactta attctgtttt gacactgtca gtgttgaagc tgaatcctaa
    42481 aatcggtgaa gtttctgcat cagcaatcga ttgatcacgt ttggcaatca tgtcatccgt
    42541 aatagactct tgtgtatata gaatcttgtt gtttcctgta ttccaagcag acatatgtgt
    42601 atttggctga taaccatcta gcaatttgat tttatcatct gaaccatcga ctttttccca
    42661 tgcttctccc tcgatccccc aaacaagccc attcaataat tctggatcac tgttaagcag
    42721 ccctagaagt tctactgatt tttctttatt ttttgaagta tttgatacga caaaatttgc
    42781 catttgtgct tgagctgtag attttagtgg aacagtaatt gcttttgaga caagttcttg
    42841 ttttgctgca tttgtcaaaa ttgtatcgcc ataatcatac ggtccttgtg tttctccacg
    42901 cattagccaa gtatttcctt ctagtggata atccttattg ctcgttgcag cgtcacttgg
    42961 aatgagtcct tgtttgtacc agctatgcat tgtacgtaac agtccgatat acgtttcatt
    43021 atcgtattgg ttgatgattt tcgaatcatc tccatccaga tcaatagcaa atggtaacgt
    43081 attccctaaa ggataatcaa agttcccttg gactttgaag ccttgtccaa tggcaaaagc
    43141 tgccgtgttt ggttcttttt catggaattc cttcaagact ttttctgcat cagcgtaaga
    43201 ttggattcca tcaattgaaa gatcgtattt atctagtaaa gctttgttaa aagtaagtac
    43261 ttgttgagca aagacatttc cattgactgg gaaagcatat aattttccat tgaccagatt
    43321 tcctttaata tatgcttcat ctaaatcatt ataagctttt tcagcgtact taggcgctaa
    43381 ttcagtcaaa tcagcaaagg ctcctttttg ggcattggaa acatagttgt tcgcaaaggc
    43441 gatatcataa ttttccccgg aagaaatgat gacgttcatt ttcttttcgt aatcgcccca
    43501 gccaatgtaa ttgatattga cttttacacc gatctctttt tcgattcgtt tgtttgctac
    43561 ttccatcaat tgatcgaaat tatctggttt atctccgatt tgatacatct gcaaagtgtc
    43621 cgaattgcta gcttctgcct ggtctttaga accagaacct ccgcatgctg ctaatgttcc
    43681 caacaacaac gctgaaacac ctgtaaaggc tagtttcttc cataacttca tttttcttcc
    43741 tcctaatttt atgatttata tttttgtttc tttattcttt tactccgccg attgttagac
    43801 ctttgacaaa atgcttctgg aaaaatggat agctgatcgc aataggcaac gtcgagatga
    43861 ctacgatcgc cattctggca gattctcctg gaactgaggc taatccgcct gtcaattggc
    43921 ttcctgctcc tgaattttgt gtcagatatt gaatattact ttggattttc atcaaaagat
    43981 actgtagtgg aactagctta tcatcttgga tgtatagtaa agcattgaac cagtcattcc
    44041 agtaacccaa tgcggcaaat aaactgattg tcgcaatacc tggaacagct aacggtaaaa
    44101 cgatctgaac aaatattcgc agttcgctag ctccatcgat ccgtgccgat tcaataatcg
    44161 aatcagagac cgaacgttta aagaatgtgc gcatcacaat aatattgaag ggactgacag
    44221 ccattggcaa gatcaatgcc caaatcgtat cttttaactg aagaagattt gtcataacaa
    44281 gataattggc caccatccct ggtgagaaca acatcgtaat caaacaaaaa acggtaaaaa
    44341 aacgacggaa tggaaagttt gaccgtgaaa tcgcataagc atataaagaa gtagccgtgc
    44401 tattgatcag tgtacctaaa acagtaacaa gtacagtgac gaaaagcgct tggaaaattt
    44461 tatcctgcat ctggtcgaac aagtacgtat aaccaaatat actgaacttt tctggccaaa
    44521 aactgtaccc gtgagtcgac aatatggact catcggtaaa agagatggcg ataacaaata
    44581 taaatggcaa aatacaggaa atagcaaaga gtgcaatcat tatattagag aaaaaattag
    44641 ccgtcttatt gaaagagcgg acttggactc tctccacttt cttttttttc atttttttcc
    44701 tccttagaat aatgcagaat cgctatcaaa acgtcgggcg atcgtatttg tgatcatcaa
    44761 caagacaaag ccgacaactg attggtaaag tcctgcagca gccgtcatac caaaatctcc
    44821 tgtggatgtc aggccattgt agatatacgt atccaaaact tgagtcacat catataatgc
    44881 accggaattc ctaggaatgt tataaaacag gccaaaatca gctcggaaaa tgtttcctac
    44941 ggctaaaatc gttaaaatcg tcattagtgg aataagttgt ggtatcgtca catgtttgat
    45001 ctgctgccat ttgctagctc catcaaccat agcagcttca taataagtcg gatcgattcc
    45061 cataacagat gcaaagtaaa taatgctgtt ataaccaatc cctttccatg taccaatgaa
    45121 caccaagata aacggccaat atttcggttc attataccaa ttgacagctt ctcctccatg
    45181 tccagtgatc cactgattga atattccttt gtcaggactt aaaaaagcgt aaacaaagta
    45241 actgataatg acccaagata gaaaataagg tagtaaggac atcgtttgat aaactttaac
    45301 gagtcgttta tttctcaact cgctcataat gattgcaaat acgactgaga tcagcagatt
    45361 caaaaggata aatgctacat tatataaaat tgtattttta gtgatgagaa atgcttgatc
    45421 cgaagaaaat aaaaatttga aattttccca acccacccaa ggactttccc tcaagctagc
    45481 caagaaccca tctgctgaaa tatgaaaatt tttgaatgct acgacattcg ccattacagg
    45541 tatgtaaaag aaaaaaatca tccaaagcat acccggtaca gccatcagga caagtgcttt
    45601 ataacgccat agattcataa aaaaatgatt gaaccccttt cgttttttca tctaaaaacc
    45661 cctttcgatt cattcgcaac gtaattatag cgctttcaat aatgagaata aataaacaaa
    45721 gtatagggaa aatgaaacaa agtataggag aggttgtgaa atcagcgttt tggcctgagt
    45781 attagagaaa aaaacgggaa aatagttcct catattttgc cgttttttga gctaataccg
    45841 agggtcagct gattgaacac cgtttatcag aggttgtgaa ttcagcgttt tggcctgagt
    45901 attagggaga tcgtgtcctt actatctaac tctgagtcac aaggaacaat ggggactgta
    45961 acaaaagtct tgtcacagtc ccttattccg aacaaacggt gttccagaag tagcttctac
    46021 ggaaataagc cgaaatttca caaaaaagcc atccgaaagt cagacggaca agtctgcttt
    46081 tcagatggcg ctacaactac tctgcttatt cttttagcta tccattcaat tatttttctc
    46141 tcacttgttc tagctcttct ttggtcatgg gtcgatattg ccctttattc aaatctttag
    46201 gcagccaaaa ttttcccata cggattcttt ttaaatataa gacttctttt cctactgctt
    46261 tgaccattcg cttaacttgg tgaaactttc cttcatgcaa aatcagacgg ataattgaag
    46321 tttctgtctc ttgatctact tgatcaattt ggagttttgc cggcaatgtc tgctcatcgc
    46381 catcaataat cagtccttct gcaaataaac ggatatcttc ttctgtcatt ataccgctaa
    46441 cctcagctag atattctttc tccacatgtt ttttaggtga tagtaactga tgggcaaaca
    46501 ccccgtcatt cgttaaaatc aagaggccct ctgtgtcttt atctaatcgg ccgactggaa
    46561 aaagatcttc ccgataatcc tggtcttcca ataaatcgat tacggtttga tcatgattat
    46621 cctgagtagc agaaatgact cctgcaggtt tatgcaaaat gtagtaaaaa tctttttgat
    46681 acgtaatgat ttctcctaaa taagtaaccg catcttcgaa ttcatttact tgcgctttat
    46741 cgcttttcac cacttgtcca ttgacttcga tcgttccttt tttgattaat cctttgactt
    46801 ctttgcggct gccaagtccc atatctgcta aaaacttatc taaacgcatg cgtttccccc
    46861 atttcaaaag agcatcaatc tagctcttct gattcactta ttttattttt aatttatgtc
    46921 gtaaacgcgc tattcctggt cctagtagtc tttctgccaa gcggattttc aaagctaaat
    46981 aaccatacac taaagcccct atacctgcta ctacaagaat caacaataga gactggaatt
    47041 tgctatcttc gtttaaaaaa gcaccaaaaa tcatctttgt gatccacgaa gccaaaagca
    47101 taatcaaggt aagaataaag atcagcaatg tcctgcgcca gacaaaactt ggtttaaagc
    47161 gggctacttc atggattctc tttaagatca aggtacaaga cacagcaaat ccgatcattg
    47221 tcgccaataa agggccgtat acttcaaata aacgaatggc tgggtattga agaaggagtt
    47281 tcactagaaa cccaataacc aaataattaa ttgccgcctt gttttcaaac attccttgca
    47341 gcatgttgga aactagcata tatagtccta ggaagagtcc tacaaaactt gcttggatca
    47401 gtacattact tcctagagaa tccggtgtat aaaataatgt atttagcgga taggcaagta
    47461 acataacacc aaatgtcgcc ggaaacataa aaaatgaaaa caactgtaaa tttccgcttg
    47521 tcaattttgc cagacctact cgatctttca acgtaacagc ttctgtaatc aacggtaacc
    47581 cagtagacgc gatcgatgtt gccaatgcaa taactaccat cgtcaactta tctggattgg
    47641 cactaaaaat cgaaaaaaga tccagtaact gtgcattgga atactcggtg gatgcagaca
    47701 tgatcttcat aaaagtgaac tgatcgacta atttgaagat cgttacccct gaacccacaa
    47761 taataaacgg aatcgcttct ttaacggtat ctaacaacaa ttccttcgtt gcaatattta
    47821 ttttgttttc gctgtagtga ataaacgccg atgtatacgc ttgatgtttc ttcaaaaaat
    47881 acaaaaggac aagaatactt gctagcatac cgataaatgc cgcgaaagtt gattgcgtga
    47941 ccgccgtcac atagtcacca tccatgactt tcatgatgat gaatgtagcc aatagcatat
    48001 aaaaaatacg tgccacctgt tctacaatct gcgacagcgc ataaggcatc atttcttgat
    48061 tgccttgcaa ataccctcgt atcacactca tacaaggaaa aaccaataca gcgatactca
    48121 aagaacgcat cgttggaatc agttcttctc cgccaccaga agcatgagct agccaaggag
    48181 aggcgatgta catgaacaaa gcaaacaaca ggcctaaccc gcccatcatc tgtaatgcac
    48241 ggataaataa tcgtcgtgaa gtgccatatt cattcaatga attatagcgt gcagtttgtt
    48301 ttgcaatcgc agcaggtatt cctgctgtag agatcagtag aaacaatgca tatatattgt
    48361 atcccatatt gaataatcca ttggcagcct tagcattttc gcccatccaa gcataccagg
    48421 gaatgatata gacagcgcct agcaatcttg aaatgatatt actggcggtc atccaagcag
    48481 agcctcgtgc cattttttct tgcggtgtta attgatctaa tgctggtttt ctttgattat
    48541 ccatcggtca gattccaact ttctacaaaa cattactatc attttaattg gtaaaacaga
    48601 aactggcaat cgattttata taacttttat aagaacaatt gtttttttga attcggtttc
    48661 gtttttttgg taaagtaaag aaatatacag ccttttatgg ctaacattta taggagatat
    48721 caatgacaca agaaataaaa aaactacccg ttatattttt aggtcttttc tttatcacag
    48781 ttggattgaa ttggttcttg ctccctcacg atatagcttc cgccggagtg ggcgccatcg
    48841 gacatttagt tgaaacggcc ttttctatcc aacgctcagt tactgtttgg agcatcaatc
    48901 ttatgatgct gcttctagcc gctgtcctat taggaaaatc agtatttttt aagactgtag
    48961 cgggaagtct gctttttcct gttgttttag agattgttcc aaaagtggaa atgatctcct
    49021 cacatttctt ttcactgatt tcaggaagtc ttttgttcag tcttggggtt tacacattgt
    49081 acacagtcgg ggcctcaaat ggcggtataa cgataccgcc gattattttc cacaaatttt
    49141 ttcggcttcc aatgccgcaa ggacttttac ttaccaatag tcttatcgtc tttttgaatt
    49201 attatgtttt tggattgttg gagacactat tcgttctcct atctattaca gtcagttcat
    49261 tgtttatgaa aatactcatc cgcttgtccc ctgtgtcaag aaaatttgaa taaagctgga
    49321 ttttgtcata gcggaaacaa acgatatatt aaaaagagtg tgctataata aaccaagaaa
    49381 ttgaataaag cgagggatca atcaatgagc ttctctttag aagacatccg tcatttacta
    49441 ttaaaagaaa atctattaaa agaatttgtt tcaacaagag gctggcatct tgatgtacca
    49501 gaaaaagctg aatttcttca gttaagttat gattcaagaa aagctgatgc ttctactttg
    49561 tttttctgta aaggaatgaa tttcaaagaa gaatatttgg attcagcaat cgaacagggt
    49621 attcaatatt atgtatcaga acaaccatac gaaaattcag cagtaggaat catcgtaacg
    49681 gatatccgta aagcaatggc gcttcttgct atggcttttt acgactatcc gcaaaataaa
    49741 ttgaaagtga tcggattcac tggaacaaaa ggtaaaacaa ctgctgcgta ctttacaaaa
    49801 gcaatcttgg atcacacaac aaataaaaaa acagcattgc tttctactat gaatacaacg
    49861 ttagatggca aaacatattt caaatcccat ttgacaacgc ctgagtcatt agatttatat
    49921 cgtatgatgt cagaagcagt tgaaaatggt atgactcatc tagtgatgga agtttcttca
    49981 caagcttaca aaacacaacg cgtatacggc ttgactttag atgtcggaat cttcttgaat
    50041 atctcgcctg accatatcag tccgatcgaa catccgacat ttgatgacta tttctattgc
    50101 aagcgacaat tgatcttgaa ttcaaaaaca gttgttttaa atcatgaaag tgactatttt
    50161 gatttactta gggaaacagc cgatctattc cagattccaa cgatcactta tggtcgttcg
    50221 gaagaaagca attatcaagt gatccgttca gataaaggta ctcacggttt tactttatct
    50281 tctcatgaag atcgcttagc tatctgcgat actgcttatg atattttgct ggctggagga
    50341 ttcaaccaag aaaatgcagc cagtgcgatc attgccgcag cactaacagg tgcctccagt
    50401 caagatgcac aagacggatt aaaagaagct cgtgtaccag gccgaatgga tcaattgatc
    50461 caatccaatg gagcgcatgt ctatgtggac tatgcacata attatctaag cttgaaaaca
    50521 ttgctgaaat ttgccaaaaa tgaacatcca gatggccgtg taattgttgt attaggcagc
    50581 ccaggaaaca aagcaatctc ccgtcgacat gattttggaa aagtgctatc agaaacagct
    50641 gacatcgcct ttttgacggc agatgaccca gcatttgaag atccgaaaaa aatcgcggaa
    50701 gaaatcaatg aagcaattac taatccagat ttgatcgttc attatgaaat ggatcgtcct
    50761 gaagcgattc gacgcgcttt agcagaaagc gctccgcaag attcagtcgt tattgcggga
    50821 aaaggcgtgg atccctatca aaaaatcaat ggtgtagatg aaccttatga aggggattat
    50881 gcaatcgtga agcggttgat tgaagaataa tggtatttat atactagtgg tttcccattt
    50941 ctaagaaaaa aagggactgt gattttttgt cacagttcct tttttcttag aaatcatttt
    51001 aacttttcac tagctaattc tgctgccttt tctctttctt catttaataa tttattatca
    51061 tagaatttga taaatgggaa ccagataatg aatgcgatga ttgcacaaat gactgctaac
    51121 acagcagctt tccaatcacc accactacca acaaatgctc cgatcccgac tggtgaaggc
    51181 cacggcatct gtgcaagcat cggtttgacg atttccagcc gaatggcaaa ataagcgagt
    51241 gtagcagaag ccatcggagc taagaaaaac ggaaccgcta aatagggatt gtagacgatt
    51301 ggcatcccga aaatgattgg ttcattgata ttgaataatc ctggtacaag tgaggctttt
    51361 cctaacactt tcagctgatc agatttcgcg aggaatgcaa taaagatgat caatcctaaa
    51421 gtcgcacctg atccaccaac ggttacataa gaattgttga attctcctgc caacggataa
    51481 tttgcgcctt gtgcgttagc tgccatatta gctaatacga tcggggtcaa gaaagaagtg
    51541 atgatattcg ctccatggat ccctacgatc cacaatgcat ggatcaagaa ataaatcacc
    51601 atgacaccaa gccatgtatt cgtcaactgc gtaacaaacc caaatgggat agcaatcatt
    51661 ttgaagatat ccgttcctag tgcaactaat aatccattga tgatcatcac cacaaacgca
    51721 atgacaaatg ttgggatcag agcagtgaaa gaacgagaaa ctcctggcgg aacaacatct
    51781 ggcattttta tgacccaatt tctcttgaca cacatacaat acaattcgac cgcaataatc
    51841 gacataataa tagctgtaaa gattcctgaa gttcctaaac gttcgacaaa agagcccatc
    51901 ctaataccat ttactacagt ttctgtctca ttgatagaag caatcaagct gattttacca
    51961 tcagtcatga tcaattcagg gatacacata aagaatgcaa aaacagatag taatgccccc
    52021 gtgagcggat taacatttaa tttttgttca tctcgttcaa ttgacgttaa ttcataagca
    52081 aacacaatgg caaagtaaat agataaaatc cccatcgtca ttgtattgac gatcatgtac
    52141 aagtcactaa ctttgaaaaa agttgcatta aaaaatcctt ctaaaaatgg gaacggtaat
    52201 ggcaaaatat ttaaaaccaa aaacatcgag cctacaatcg tgaaaggaat agaagccatc
    52261 cccgctgcca tgacagcccg aacgatttta agttgcgcaa ttttccccat aggtcccatc
    52321 aggtatttct gcaaaaaatc aaacattagt tattctcctt tcctaaatat aacgcttttt
    52381 catattgttt aatacggata tactgttggt cttggttgag tgaaattttc tttaaacgat
    52441 actgtataaa tgcactggca aaaataccgc ttacaacaag aatgaaaaca aataactgtc
    52501 ctccattgtt atttgccata aaaggataca agaatgtaaa tcctgaggta aacaacactg
    52561 gaatcactaa taaattaaag acactctgga acaaaaaata ccactttgtc catggaacgt
    52621 tcgtttggtg ttttcgataa agctttactt gttctcctac tgcaggcaaa atagctaaaa
    52681 tcagaatcat cggtaaacta cttcctaaac ttttagctga tagaagcaga atcatccatt
    52741 gcatgttcat gaaaaagaaa aaggcagtca ggtaacgtac gattagataa cgattgaaat
    52801 acatgttttt caaactaagt tgcttttttt ctaattcgat tttccttttt actgtatcct
    52861 gcacaatgat ccttcctttc tatttatttt tttgatacaa ataattcatt tcctgtgcaa
    52921 cttccaataa tgtcatcgtc gtcatcagat gatcttgtgc atggaccatg ataatttcca
    52981 tttctatttt tgtcccgctg gcatattctt tcagaagttg agtttgtgac tgatgcgcat
    53041 gaagaatctc ttcatttgct tgtcgcaact tctcttctac ttcttcaaac atctctttac
    53101 gcatcgagcg aaatgcttca tgtatcagtg tccgtgcatt tccgctatgg aaaatgatat
    53161 caaaagaagt cacttgtagt gtttcactgt ttattttttc agccatctgt tttcctcctt
    53221 attcgatcag tttgataaat ttactgctga aattctcaaa aataggttca gctaagattt
    53281 cctgctgtac ttttagctgg tcaactaacc taacgattgc tttagtcacg accgtaattc
    53341 cttcattttc gacataagaa ggcgaaacta aaaaaacaaa atgaatttct tgatattgat
    53401 cccactgcat cccagaagga atcaatgcaa cggctacctt tgtttcgatt ccaactggaa
    53461 cagcgggatg tgggactgcc actgtatcac tgaatatgat ttgtcccata ttttcacgtt
    53521 gctggatctg ctttcgcatt tctcttatat atccttcttc ttcatttatg ctcagctttt
    53581 gcagcaaatc atgaagaatt tcttcctttt tcggttcgta cgtataagtc ttgaaaaatt
    53641 tttcagacaa ctgttcggtg taaatctgct ttttttgctg tagagaaaat gctggtatcg
    53701 gtctatctga ggaatgatag ggtatagatt ctccaactac ctttcgtatt ttacgaacat
    53761 catcttcatt caaaaaaata ctgacatgta aaactggaat tttgaaaaac attgtcgaaa
    53821 gatcaataga agaaataatc aggtcgattc cttttaaagt tgtttcattg atctcataat
    53881 agccttttac acttaccact gtaatatttt taccgaattc actgactacg cgattcttta
    53941 atagttgggc actgccgtaa cccgttgcac agataatcaa tgcatgtagt ttatgcgcag
    54001 ctctttcttt ttccaatgcc gccatgagat gcaaagccaa ataggcccat tcatcttcat
    54061 tgatggtata gtttttcaaa gccggcatgt tgctgaaatg cctttttact gcgtcaaaga
    54121 ctttgggatt ttctttcttt atctcctcag tcaatgggtt ctcaatgcga atacctcggc
    54181 tcaatcgaat catcatcggt gacaaatgat cgagtagtcc attgaaaagc tggagatcct
    54241 ctactaatga aaaatgaaat tcctgagaca atcttttcag aactttcgct aattcctttg
    54301 atagttcctg atcattttga ttttctttgt gattcgactt agccatgaga tgtaatgtca
    54361 aataagcaat ttcttcttcg ggaaacgata cacctgcgat tacctcaata cgttgaacga
    54421 tccgctgcgc cacttgatac tctactcgct tagaaacttc ttgttcgatg cctaattcct
    54481 tgatttcaaa cccttctcgg agccgtttga tccccaaagc caagtgtaaa gtcagattct
    54541 gtatgataaa atccgaaact tttaacttag cttctcttgt ttcatcgaga atgataatga
    54601 ctagctcttc aaaactgatg tctgggaaaa aacggctttg ccctaaatat tctttcaatg
    54661 aatttccata atggtttcca aaaaaagtat ccatgataaa atgccgtcga tttctttctt
    54721 ccccttcgat ccatgtccct tgaccatgct tagaaacgat ttttagtgaa tatggaatca
    54781 atcgctcctt gatttcttgc atgtcctttg tcaaactgga acgactgata taaagctctt
    54841 ctgccaaatc atccataaaa agaatggcat cttccaaaag caattggttc aatatatact
    54901 tttgtcgatc ttcggactca taaaatcctt gcggttctct agcatatcca ggtactattt
    54961 cttgccgact caaaaacaag tcaaatgtca gtctttgtaa aatatgcagt tggtagccat
    55021 aacctggttt agcaatgatc ttacctccgt ttttttctac taactctttg atccgctgca
    55081 agtaattacg gattgtccga tcagataata acaattcaga agctaaacgt tgactggtga
    55141 catactcctt cttgtgatca atcaaatatc tcaacagcaa tttttccttc tgtttcatta
    55201 tcatccactc ctattccatc atatgttctc ttcgatcacc tctgctaact tctggatgcc
    55261 tgtagggatt gggacatacg cttggaaagg gatactaaca acaggctttc caacagattc
    55321 tcctaatttt ttgaatttat ccaaaaacat cgtcgtttgc gggctgatca gaaataaatc
    55381 aaaatcacta ttcttaatca tattatcgcc ttctgtagct gaaactgcat ctacgagtac
    55441 ctcttctctt ttttgcttaa agaaatccgt tgtctttgag gccatcatcg aggatgacat
    55501 tcctgccgca caaataatca atgctttttt cataaatcaa tcactccttt tatttttata
    55561 aaccaagcat atcttgtaag cgttttctat tcaaaatata tatttccgta gcattgcgga
    55621 aaaaatgtgt atcatatctt gaataataat ttctggcacc gtgatatact agtgatagaa
    55681 ttttttcagg aggggattta gtgagtatca cagtgaatat tttgtataca agtacaaatg
    55741 gacaagccag aaaatttgca gaagaaatgg tcgaaagaaa cattgtcaaa gcgattcgtg
    55801 cagaagaagg aaacgagaaa tatgactatt actttccact agaagatcca gaatctcttt
    55861 tactgatcga ccgttggaaa gatgaagaag caatcgaaaa gcatcacaaa tccgaaatga
    55921 tggctcaaat aaacgaactc cgtaaaaagt ataaattaaa aatgaaagtc gaacgtttca
    55981 ctgatttacc gtaaaaagat acacatggaa tgatagcaat ttatcatcca tgtgtatctt
    56041 cactttttcc ttatttttta ttcgatctca tctaatgatt caaaccgtgt ttttctctta
    56101 tttttcggat aagtatttca gtcaccccaa tatacatgat tcctaaacat accccaagga
    56161 tattctcaaa aatgcggatc gttactgctc ctggaacccc gaataaactg gcagtgattg
    56221 ccaaagcacc aaaagaatta aaaactgttt tatatctata ggtagaacaa acacctaaag
    56281 ctagaccacc taatataccg acccagttaa aaggaataaa ttggctgata ccaataaata
    56341 aaacacatcc aatcaatgag ccaatgattc ggtctactgc tcgttctttg aaaccaatcg
    56401 acatcaatcc atagctagat acaatagaag aaaatgcaaa ccctgcccac atcaatcgct
    56461 gaaaagggac atactctcct gcaacgatca aaagactaat gccaaatgca tagatgagca
    56521 tccaaatttt ttcctgcgaa tacatgcctt tcaaaaaaat ctctttaaac aagcttttgt
    56581 ctttgttctt ttctcgatgt tttctgtaaa ggagaactga aaaccaacaa aaaaacaaaa
    56641 ataataagct acttgtttgt gtaaagaaat tcttgttcaa cgaatcttta ggtaacgaat
    56701 aaacaataaa gagatatgaa aagccgtaca aagaagcatt ccccattttg ggatcactag
    56761 cggttgccat taatatgctg cttagtaaaa taaaatgaat caacatgccc ataactgacc
    56821 atttgatcag ctggacatac ggtgctacta atagtgagaa catgacgatt gctaaaccta
    56881 tgatcgaatg ggaaatctta taaccaaagt caacaaaacg aatactcatc aacatacaga
    56941 ataaaaccac agcgtatggt ttatgcttcg attcaaataa tgttgtaatt ataatgataa
    57001 aacaaatggc aaacaatacg atgaataatg atcgtaaaaa caatgaacaa atatacatgc
    57061 acttttcttt tttagaagct gcttctcgta ttttttgttt taaaataaac ggatctagtt
    57121 gtagagactg ataaaaatcc acctgttgtc gtcctttctt tttactttta ttttttacag
    57181 tcatctatga caggttgtaa atactatgga acatcatatg gcgttgtcat tttcttgtca
    57241 actatttttc ctattcatgt ttttataaaa aaacatatcg ttttcttgta tgattaaatc
    57301 accacaaaat aaacaactga gagaacgata tgctttatga atggttctat caaaaaaatt
    57361 ctgagatcaa tagacaaaaa tttgatgatt acagaggtct cttacgaaac cttacagaag
    57421 aaaaaaacat tgatcgttgc tgcagttctc ttccctgctc catgtgtctg tagaaattgt
    57481 ggttctgctg tggtagatgg aaataggaaa ttaatggtgg tgaaaaacaa gaagaaggaa
    57541 accattgtcc actttgaata atataatcat atgccaatgg ttatacgcct aaaaaagcag
    57601 cgctatacct gtaaaaactg tcgaagccat tggaacgctc aaagttattt tatccgtcca
    57661 aggcactcga tttcaaatca tgtcagacat aaaatcactt ctttactgac tgaaaaagtg
    57721 tcgttatttt ttatttcgaa aagttgttag gtatctttga caaccgttat tcgcacattg
    57781 aaagagttga gaagctattt accaaagtct tccaaaagaa ttctgtcttg agtgttgagg
    57841 gttgtgaatt tcgttctcac gtttccgttg aggataaact gagttttatt tccgcggatg
    57901 gcgagacaga aaaattaatc gagaaaattt acctacacaa aaaattcctc gattaacaaa
    57961 ctatttcctt agctgcacta atcctaaaga aatagaattc ttagttacag acatgaacgc
    58021 cacctacttt cagctcacca aacatgttct accaaatgcg aaattggtga ttgatcgatt
    58081 acttggtttt tccacgcctt tcaaggaggc gtaaccttta ttcacgagtt ggttgaagct
    58141 ttccgagaca aaagacccga cttgtttttc tccttattga caaagagcca ttaaaactag
    58201 agaaaaaatt acaactgatc gctgattatt tgtagaaaga aaatcaaagt attacccaat
    58261 aaacttgagt cattaggcaa aaaacactgc gaaactcaag aatgattttc gcagtgtttt
    58321 tgtatatttt ttattaacta gtattactac aaaggtttat ttttcacttc tcaacgcagc
    58381 acaaaattgt ctttacttat ttactggttg ggttcaacct tctctactcc gatactaata
    58441 tttgtttgta taccccttct aaaatcggta gtttccattc aattgatggg tactagttga
    58501 ttttaaaaac tatgcttatt caatttttta ttttagctca tatatttcgt ttctttctaa
    58561 attttatact gatgaaaaca agtagcacac ccaatattga caatataaac tggctaatga
    58621 tggtttctcc agtttttgga agatgcttat ttccactgct tgttggattg tttcctctta
    58681 tatctgagct atcagcaact tttctattct cttttgttaa aaagatcgcc acttcttgtt
    58741 ctttttcgat cttgaaagtt actggtgtcg catccaattc ataccccgtt ggcgcttgag
    58801 tttctaccag ctgatatgct cctggtgcta agccatc
//
LOCUS       DACDYJ010000008        55936 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDYJ010000008 DACDYJ010000000
VERSION     DACDYJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.008"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            238..1452
                     /locus_tag="IWM51_000486"
     CDS             238..1452
                     /locus_tag="IWM51_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP9969929.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1483..2910
                     /locus_tag="IWM51_000487"
     CDS             1483..2910
                     /locus_tag="IWM51_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9969930.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            2933..3859
                     /gene="glsA"
                     /locus_tag="IWM51_000488"
     CDS             2933..3859
                     /gene="glsA"
                     /locus_tag="IWM51_000488"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP9969931.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            4067..5527
                     /locus_tag="IWM51_000489"
     CDS             4067..5527
                     /locus_tag="IWM51_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969932.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            5748..7511
                     /locus_tag="IWM51_000490"
     CDS             5748..7511
                     /locus_tag="IWM51_000490"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP9969933.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            7595..8053
                     /locus_tag="IWM51_000491"
     CDS             7595..8053
                     /locus_tag="IWM51_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP9969934.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            8162..8845
                     /locus_tag="IWM51_000492"
     CDS             8162..8845
                     /locus_tag="IWM51_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9969935.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            8992..9606
                     /locus_tag="IWM51_000493"
     CDS             8992..9606
                     /locus_tag="IWM51_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP9969936.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            9622..9828
                     /locus_tag="IWM51_000494"
     CDS             9622..9828
                     /locus_tag="IWM51_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969937.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            10367..10924
                     /locus_tag="IWM51_000495"
                     /pseudo
     CDS             10367..10924
                     /locus_tag="IWM51_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            10982..13021
                     /locus_tag="IWM51_000496"
     CDS             10982..13021
                     /locus_tag="IWM51_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP9969938.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFINNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNPDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(13130..14281)
                     /locus_tag="IWM51_000497"
     CDS             complement(13130..14281)
                     /locus_tag="IWM51_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9969939.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(14435..14965)
                     /locus_tag="IWM51_000498"
     CDS             complement(14435..14965)
                     /locus_tag="IWM51_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969940.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(15064..15414)
                     /locus_tag="IWM51_000499"
     CDS             complement(15064..15414)
                     /locus_tag="IWM51_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP9969941.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(15571..17100)
                     /gene="ahpF"
                     /locus_tag="IWM51_000500"
     CDS             complement(15571..17100)
                     /gene="ahpF"
                     /locus_tag="IWM51_000500"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP9969942.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(17110..17673)
                     /gene="ahpC"
                     /locus_tag="IWM51_000501"
     CDS             complement(17110..17673)
                     /gene="ahpC"
                     /locus_tag="IWM51_000501"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP9969943.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(17817..18479)
                     /locus_tag="IWM51_000502"
     CDS             complement(17817..18479)
                     /locus_tag="IWM51_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9969944.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            18663..20309
                     /locus_tag="IWM51_000503"
     CDS             18663..20309
                     /locus_tag="IWM51_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9969945.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            20300..21382
                     /gene="uxuA"
                     /locus_tag="IWM51_000504"
     CDS             20300..21382
                     /gene="uxuA"
                     /locus_tag="IWM51_000504"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9969946.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(21490..23043)
                     /locus_tag="IWM51_000505"
                     /pseudo
     CDS             complement(21490..23043)
                     /locus_tag="IWM51_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            23191..24332
                     /locus_tag="IWM51_000506"
                     /pseudo
     CDS             23191..24332
                     /locus_tag="IWM51_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(24458..25537)
                     /locus_tag="IWM51_000507"
     CDS             complement(24458..25537)
                     /locus_tag="IWM51_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9969947.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(25646..26086)
                     /locus_tag="IWM51_000508"
     CDS             complement(25646..26086)
                     /locus_tag="IWM51_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9969948.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(26143..26553)
                     /locus_tag="IWM51_000509"
     CDS             complement(26143..26553)
                     /locus_tag="IWM51_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9969949.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(26734..27168)
                     /locus_tag="IWM51_000510"
     CDS             complement(26734..27168)
                     /locus_tag="IWM51_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9969950.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(27268..28116)
                     /locus_tag="IWM51_000511"
     CDS             complement(27268..28116)
                     /locus_tag="IWM51_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9969951.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            28505..30271
                     /locus_tag="IWM51_000512"
     CDS             28505..30271
                     /locus_tag="IWM51_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP9969952.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            30373..31632
                     /locus_tag="IWM51_000513"
     CDS             30373..31632
                     /locus_tag="IWM51_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9969953.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            31779..33080
                     /locus_tag="IWM51_000514"
     CDS             31779..33080
                     /locus_tag="IWM51_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9969954.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            33083..33934
                     /locus_tag="IWM51_000515"
     CDS             33083..33934
                     /locus_tag="IWM51_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9969955.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            33947..34762
                     /locus_tag="IWM51_000516"
     CDS             33947..34762
                     /locus_tag="IWM51_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP9969956.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            34913..36079
                     /locus_tag="IWM51_000517"
     CDS             34913..36079
                     /locus_tag="IWM51_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9969957.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            36309..36956
                     /locus_tag="IWM51_000518"
     CDS             36309..36956
                     /locus_tag="IWM51_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP9969958.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            37040..37984
                     /locus_tag="IWM51_000519"
     CDS             37040..37984
                     /locus_tag="IWM51_000519"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP9969959.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            38119..39570
                     /locus_tag="IWM51_000520"
     CDS             38119..39570
                     /locus_tag="IWM51_000520"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP9969960.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            39591..40427
                     /locus_tag="IWM51_000521"
     CDS             39591..40427
                     /locus_tag="IWM51_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9969961.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            40427..41086
                     /locus_tag="IWM51_000522"
     CDS             40427..41086
                     /locus_tag="IWM51_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9969962.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            41212..41901
                     /locus_tag="IWM51_000523"
     CDS             41212..41901
                     /locus_tag="IWM51_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969963.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            41907..42863
                     /locus_tag="IWM51_000524"
     CDS             41907..42863
                     /locus_tag="IWM51_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP9969964.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            42907..43419
                     /locus_tag="IWM51_000525"
     CDS             42907..43419
                     /locus_tag="IWM51_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9969965.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(43675..44223)
                     /locus_tag="IWM51_000526"
     CDS             complement(43675..44223)
                     /locus_tag="IWM51_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9969966.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(44312..45493)
                     /locus_tag="IWM51_000527"
     CDS             complement(44312..45493)
                     /locus_tag="IWM51_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP9969967.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(45729..47237)
                     /locus_tag="IWM51_000528"
     CDS             complement(45729..47237)
                     /locus_tag="IWM51_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP9969968.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            47396..47587
                     /locus_tag="IWM51_000529"
     CDS             47396..47587
                     /locus_tag="IWM51_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969969.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            47790..48707
                     /locus_tag="IWM51_000530"
     CDS             47790..48707
                     /locus_tag="IWM51_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP9969970.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            48700..49773
                     /locus_tag="IWM51_000531"
     CDS             48700..49773
                     /locus_tag="IWM51_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969971.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            49921..50439
                     /locus_tag="IWM51_000532"
     CDS             49921..50439
                     /locus_tag="IWM51_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969972.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            50420..50812
                     /locus_tag="IWM51_000533"
     CDS             50420..50812
                     /locus_tag="IWM51_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP9969973.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            50888..51823
                     /locus_tag="IWM51_000534"
     CDS             50888..51823
                     /locus_tag="IWM51_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP9969974.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            51956..52735
                     /locus_tag="IWM51_000535"
     CDS             51956..52735
                     /locus_tag="IWM51_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP9969975.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            52728..53045
                     /locus_tag="IWM51_000536"
     CDS             52728..53045
                     /locus_tag="IWM51_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9969976.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            53289..54899
                     /locus_tag="IWM51_000537"
     CDS             53289..54899
                     /locus_tag="IWM51_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9969977.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            55056..55904
                     /locus_tag="IWM51_000538"
     CDS             55056..55904
                     /locus_tag="IWM51_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9969978.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        18549 a         9923 c        10900 g        16564 t
ORIGIN      
        1 ttccttatga aaaaatatct ttaaaaataa aaaaagacga aaataaacgg ttttctataa
       61 aaagaagacc gtttattttt tgtctttatt tgtgtgaaaa acgaaaaaga gcacaacaca
      121 tcaagctttt attaattgtc tgtcttttat aaatgcaagt attaattcaa aaaaacagaa
      181 gaaaaaaaca actacacctt cttttatact cgtaatcgta gaaaggaggc gaaaaaaatg
      241 aatacattac ctatcaatac aggagataca gcattcatga ttttatgtac agcaatggtt
      301 tgcctgatga ctcctggtct agcttttttt tacggcggat tagcacggaa gaataatata
      361 ctaacgatca tgtctcaatc ttttctctcc gttgggataa caacgattat gtgggtattt
      421 ggtggattcg gattggcctt tggacgggat atcggcggtg ttattggaaa cccggcagat
      481 ttctttttga tgagacatgt tacaagcttc cccaacatgt ttcatggtgc aacgattcca
      541 tttttaatgt tctttcttta ccaactgatg tttatcgtga ttaccgttcc tttgatgaca
      601 ggagcttttg cagggagatt gaatttaaaa ggctatatta tacttgttat tttttggaat
      661 ctgctgattt atttccctgt ttgccactgg atatggggtg gcggattttt ggatcagatg
      721 ggatttagag atttcgctgg tggagcagtc atccacacga cagctggatt cggctcttta
      781 gcatgtatcc tcacacttgg tcaaagaaag attcctttag gagaaagaag tcagcatagc
      841 agtcttatgg cagctgctat cggtacagga ctgctttggt ttggctggtt cggttttaat
      901 tccggtggtg cacttcgagc agatgaacaa gcagtcaatg catttggttc aacattcatc
      961 gctcttgcat ttgcgatgat tacttggctg atcattgcaa aagtaaaagg aaatggcttt
     1021 gattttgtcg acgtactaac aggatccgtt gctggattgg cagccattac gccttgtgcc
     1081 ggttatgtag aagcaaaaag tgccatgctg attgggatca ttgcgggtat cgtctgtcat
     1141 gctgctgttg attttcgtaa aaagaaacaa tgggatgatg cacttgatgt gtggggtgtt
     1201 catggcatgg gcggattcac aggtacgatt ctgataggga tctttgcatc taacagccat
     1261 ttactaacga atccaagcag ctggtacttc ttagggattc aagtgattgg tgtcatcatt
     1321 actgccgtgt atgcctatgt gctgacaaca gtgattttaa aatcggcgat gcactttaca
     1381 acgatcacta ctacaaaaga agaacaagaa gaaggattgg actttttctt ctttggcaac
     1441 aaactcaatt gaaacaaatt caaatagaaa agaggaagat tcatgcaaca aagcaaaacg
     1501 aaaattcctg caaaaaaatt aacgttcttt ggctttttgg ctatgaccat ctcaatggtc
     1561 gtttcattat atgaatatcc cacattcgcg acctcaggct tcagcttagt atttttcttg
     1621 ttattaggcg gtttgctatg gtttatccct gtggcattgt gcgcagcaga gatggcaacg
     1681 gtgaaaggat gggaaaaagg cggtgtctat acttgggtca gtcgcacatt gggaaaacga
     1741 ttcggttttg ccgcaatctt tttccaatgg tttgagataa cagttggcta cttgaccatg
     1801 ctgtatttct taactggtgc attatcctat gcaacgggta ttggtgctat acaaaacaat
     1861 aaatatctga aattggcaat cctattgatt attttctggg ctattttact ctcacagtta
     1921 aaaggaacca aatacacttc attgattgct cggatcggtt ttgtagctgg tatcttgttg
     1981 ccagcattag tcttgtttgg cttagggatc cattatgtcg cttcaggcgc tccactacaa
     2041 acgatactgt ctatgaaaac attgattcct gatttttcga aattgcctac gctggttgtt
     2101 tttgtttcct ttattttagc atatatgggc gtagaaacat ctgccagtca tgcaaatgaa
     2161 atggaaaacc ctaaaaagga ttatccatta gcaatgtttg tattggttat tctggcaatc
     2221 attttagata cttttggcgg attgaccgtc gctactacag tacctcaaca tggtttgtct
     2281 cttaatacag gggtgattca atcgttagaa ttcctattaa gacacattga tccaaatctt
     2341 gcatggattg cgaaaattct tgcgattctt gtttgtttag gtgtcatcgg agaaattgct
     2401 tcttgggtaa cctctccttc aaaagcactt cacgtagcag cagaagaagg attacttcct
     2461 gaatattttg caaaagaaaa cacacacggt gttcctgctc atttgatgat cgcaaatggg
     2521 atcgttgcaa caatctgggc agccgtcttt acattaagcg gcggggggaa taatctttca
     2581 tttcttgcag cgatgtcatt gactgttgta atctacctga tgttgtactt tatgtttttc
     2641 gcgggctatt tgaagctggt ttttaaagat gacagtcttg aaagagccta tcaggttcca
     2701 ggcggaaaag ttgtaaaagt aatcgttgct tgtttaggtc ttttgacttc actttttgct
     2761 ttttgtatct catttgttcc acctgctagt atcgcagctg gacagcatgg gatttatgaa
     2821 acgattctga ttgtttctta tcttatcgtc ttagttattc catttacgat ctacgcatgc
     2881 cgtcataaat tccagcatgc agaaaaataa aaaatgaaag ctggttattc aaatggaagt
     2941 aaaagaatta gaacaattga ttggaaaaaa tttccactat tatcaaacag gacaagtggc
     3001 ggattacatt ccagcattag ctgaagtgga tcccaaacaa ttaggtatgg cgatttatga
     3061 tttggataca aaacaactga ttgagggagg agacgccgat gtgcgctttg ccatcgaaag
     3121 catgtcaaaa gttcccgtat tgttattagc aatcaaagac aacggaatag aaaaagtctt
     3181 tcaaacaatc aacacggaac caaccggttt tgccttcaat tctatcatga atatggaaat
     3241 caatcatcgc aaacatccaa tgaatccttt tgttaatgca ggagcgattg caacaacctc
     3301 gttgattgcc ggtaaagatg cagatgaaaa atttgagcga atcttgtcat ttatgaaaga
     3361 gatctgtgat gatcctaata tcacattgaa tgaagaaatc tatcattctg aatcaagaac
     3421 aggtgatatc aatcgctctc ttgcttacta tatgaaaggt aatcaaatga tcgaaggaga
     3481 cgtaccggag atactcgatg cttatttcag acaatgctcc ctcaatgtca ccgctacagg
     3541 catagcaaaa ttagctgctg tgctggcaaa taaaggaatt gctccttgga atggcaaaca
     3601 aatcattact gaagaaagtg ctacaatcgt caaatcaatt atgacaactg ctggtttgta
     3661 tgatgaatca ggagagtttt ccgtccatgt tggtgtacct gcaaaaagcg gtgtaggcgg
     3721 cgggttgatg gcagctgtgc caaatcgtta cggcataggt gtcttcagcc cggcactaga
     3781 tccatttggt aacagcgcag ctggtatcca attgttgaaa gatgtagtca aagaactaga
     3841 cgctgacatc tttgaataac cattcttgaa agaggctggg gtaaaagtcc aaacggctca
     3901 aagctaccta caatgaggct gaaattgtga gcttgtttga cttttgtccc agtttctttt
     3961 tttattggtt tttgaaaggg cttttctaaa taaaaaggaa acaaaaatac gatttacaga
     4021 tatgattaaa taaaatcata agaaaaactg gaggcgtgta tcaggaatgc tggaaaatta
     4081 catcgagcga aatatattta gaaaagtgta tctgtgtgaa caattatttg aattccaaga
     4141 aatagacatc gaacaaacag ctatttctct aagggtgact acaccaacga tccttcacga
     4201 tctagaaagt ctagccgagt gtctagaata ttgtataaaa gaacaagtaa gagagaagca
     4261 taagtataaa ctcgtattca aacatggtat tgctctttct gagctcacac agtttctgta
     4321 cggtcaatct tattttttaa agtttttaag ttattatttt aatggtatat tcacgagtac
     4381 agaactggct gatcttgaat ttatttcttt gagcaaagta tatacaatca aaaaaatcgt
     4441 attggatttt tttaaagaaa atgattattt gaaaaataaa gaaatcatta tcccagaatt
     4501 tgactcgcgg aacatcttgc tagctttagt acgttatgtc aattgggaag gctatgaaaa
     4561 taaaaatccg tatgtagaag aagcttgtaa cgagttgatt gattatgtag agactcattt
     4621 ttttaaaaga agatattcgg aagatgaaaa gtcctttatc atacgaggaa tagagatcgc
     4681 aatcggaaga aaggcatttc cccttggact gaaagaagaa gataagaaaa gcgcagaaac
     4741 gaagccgtta tttcaattag tctgtcaagg attggaagag caaaaaggaa ttctagactt
     4801 acaagaagaa gatgtctatt atattttttc tttgttcaac agtagaaatt ataccaatga
     4861 gaacatggag ctgttaagaa aagatttcga agttgtttat aaaaattttg ttctcaatga
     4921 cctatcactg aatgaattga ttttacagat tattagtatc gctgaaatcg acgatgcaga
     4981 aaattttatt ttcaagcaat cctttttaca atttgtccgt acgttgtggg cagatggaca
     5041 agtattttta ccggaaagga tctatcttct atcagaaaaa gaacaacttc tgtacggaaa
     5101 aattgtagaa atattggaag agtggaaaaa gaaaaatcaa atccaactaa gatggaatga
     5161 aaatttcatt cgaaaattcg tcaaaagtct ttctttgatc aacagtgaac aagcggggag
     5221 acaggaaata gaaatttttg ttgtttcaga aagttcagtc aaacaatttt tttatcgaac
     5281 ccaatttcat ttgtatgcgg aagaaggcat cgctgaaata aatcctattt tatttcgttc
     5341 attagaagaa ttgcctgatg aatgtctatg tgcgaaaaaa agagtggttt tgtgcgatgc
     5401 tgctagctat caaaatggat tagaaactga aaagacaaag atctatccaa tctcattaaa
     5461 aggtgcaaac atccatttgt atcatattgc tcaggaactt cacctattaa aaaaacatat
     5521 ttcttgatga gaccttaatt aggagacctt ctacaaagct ttttaattgt ctgtagaagg
     5581 tttttatttg agtcctgatt cgtgcgaaga tctacttata taacaagctt tctctgaaat
     5641 ttttttaaaa tgctattgac ttagagtcaa ctctaagtag tagaatagat aaagtgtaac
     5701 aaatctattt tcattaaaag aagatagtac ggaggagaga atgtattatg agtacagtat
     5761 tacatgtatt ggatgcaatc aatagccccg cagatgtcaa aaaattatct gttgaggaaa
     5821 tgaaacagtt ggcagaagag atgcgccaaa aaatcttgca aaaggacagc gatgtaggtg
     5881 ggcacgttgg tccgaatcta ggtgtagtag aattaacgat tgcgtttcat tatgtatttg
     5941 attcaccaaa ggataaaatc gtttgggatg tttcccatca gtcttatggt cataagcttt
     6001 taactggtcg aaaaaatggt tttgaaaaag gacattaccg tgaagtgagc ggatattcca
     6061 accaacatga aagtaaacat gactatttta cagtggggca cacatctaca tctatcagct
     6121 tagcaacagg tttagctaaa gcgagagatc tgaagaaaga atcaggaaat gtggtagcat
     6181 ttatcggtga cggctcccta agtggtggct tagcttttga aggattgaac aatggtggtg
     6241 ttttacaatc caatctaatc gtattagtaa acgataatga gatgtctatt gatgagaatc
     6301 atggcagttt atataaaaac ttagctgagc ttcgtcaaac caacggtaaa agcagcaata
     6361 acctatttaa agcaatggga tttgattatc gttacgtgga taatggaaac gatctggaaa
     6421 cggtgatccg tttatttgaa gaagtaaaag atattgatca tccgattgta cttcacgtcc
     6481 atacggaaaa aggtcgcggt tatcagccgg caatcgataa taaaatgaaa tatcattggc
     6541 atgtaccatt tgatctgtcc accggggagg caaagcaagt aacttctgga gaaacattta
     6601 atatgatcat catggattat ttggataaga aaatgcaaac aagtacagct cctattgccg
     6661 cagtcaatgc agcgatacca ggcgcctttg gactgaaaac attccaagaa aaacatccag
     6721 atcattattt tgacgtgggg atcgctgaac agcactctat cactttcgct tctgctttgg
     6781 cttctcaggg agtacgtcca gtcgtcttcc aatcaagtac tttcttgcaa cgggcatatg
     6841 atcaattatc acatgattta gcgatcaacg agaatccagt gattatttta gtcggtggcg
     6901 gaacgatctc agctagtgac cgaacacacc aaggaacatt tgacattcct tacttatcaa
     6961 gtatcccgaa catcaaatat cttgctccag caacaaaaga ggatatgatc tctatgctag
     7021 attgggcgtt cgaacaaaca gcgcagcctg tagcaatccg aattcctaca catggtgtag
     7081 aacatggtac agctatcgca aatgattata gtattccagc ctatcaaaca gtaaataatg
     7141 gaggaaaagt agcaatttta gctttaggcg gttttcttga attaggaaaa gaaacagtaa
     7201 aagcttttag aaaaaaagga atcgaagcaa cgttgatcaa tccaggaacg atcacagaaa
     7261 atgacgtaga aacattagat aaattaaaag aaacacacga attagtcatc acattagaag
     7321 atggtagttt gtcaggagga tttggcgaaa atatcagtcg ttattatggt gattcttcaa
     7381 tgaacgtatt aaattttggt gcagagaaag aattcacgga tagcgttcct gtagggcagc
     7441 tttatgaacg ttatcattta acaccaaaac aaatcgttga agatagtctg cgtgtcttga
     7501 atattcaata attcattatg ctcaccatcc agataattgt ttatactagt atctggatgg
     7561 ttttttcatt ataaaaagca aaggggtgga tgttatgtat cagcaagtag aagaatatgt
     7621 caaaaagttc caagaaaaat atcctagtta tcaatcagaa aaattaggtt tcttttcatt
     7681 tggggataca gaaaaaatgg cagacgagtt gagcgaacta gttgtaaaaa agataaagac
     7741 tggtacatca agtggttacg atctgtataa acaagagaat gagcctttac caaaagttgg
     7801 tcaattagac gtggtcctga atggaaaaaa tgaaccagtt tgtatcatca aaaatataaa
     7861 tgtgaccatc ttgcctttta gtgaagtcac gaaagagcat gcttggaaag aaggagaagg
     7921 ggatcggaca ttagcttatt ggcggaaagt ccatgctgag ttttttaaac atgcttataa
     7981 agaagcgttg aacatagaat ttacagaaga gaagctggtc gtatacgaag aatttgaagt
     8041 aattttcttt tagtaagaaa ggtctgctga aagaaatagc tcattattgt ctcctgaata
     8101 gaaaagggtt acatttttat atgaagctgt atttttatat aacattactg gaggagatat
     8161 tgtggataca ctggaacaaa tcaaaggcgg agtcgtcgtt tcttgtcaag ctttggaaaa
     8221 cgagccgcta cacagttctt ttattatggg gagaatggct ttggctgcaa aagaaggcgg
     8281 agcagttggt attcgagcaa atacgacaga agatatcaat gaaatcaagc gaatcgttga
     8341 tttaccggta atagggatta tcaaaagaga ttacgatggg tcagaagtgt ttattactgc
     8401 gacagaaaaa gaagtggaag aacttttgga gacgcaagca gaaatcattg cactggatgc
     8461 aacttctcgt aaacgtccga cagaaaaaac aactgctgaa cttgtccaaa tgattcacga
     8521 acacggtcgg ttagctatgg cagatatttc gacttttgag gaagcgcttc aagcgcaaga
     8581 agatggtttt gatcttttgt ctacgacttt agcaggctat acagaatatt cacgaaagac
     8641 gcagacacct gattttgatt tattgaatca aatcatagaa caagtcgata tcccagtaat
     8701 tatggaagga catactgact ctcctgaaca agtagagaaa gcatatgaat taggtgcttt
     8761 ttcagtcgta gttggatcta ttatcacccg tccgcaattg atcactaaaa gatatgtcga
     8821 agccgcaagt aaagcaaata actaaaaaac acgcacggca cagattcttg acaaaaaaga
     8881 aaaattgata gatagtttaa agcagattct aagaagatga aaaaagcatt acttttcatt
     8941 ttgtcttcaa gatctgcttt ttaacgaatt ttatagaaag aaggcgaaac catgtttgaa
     9001 cggagtcctt cagaattgac agaacgtgaa aattacaagt tgctgattgg ttcagtcatc
     9061 cctagaccag tagcagtagt aacgactaaa tcagcaaaag atatagtgaa tatcgcaccc
     9121 ttcagttact tcaatatcgt cagttcgaat cgacccatct tatccttggc gatccagaga
     9181 aaacaagatg cagtaaaaga cacagcaaag aatattcttg agacaagaga agctgttatc
     9241 catatccttg atgaggacaa cgtagaagaa gtaaataaaa cagcagccaa tctgcctaat
     9301 gaggaaagcg aactgtacct gactgacttg actttaactg attcagtaat catttcagta
     9361 ccagcattga cagaagcaaa agttcgtttt gaaacatcct tatatgaaca tgtcgagata
     9421 cggaataaaa ataaagtaac agctgattta tttttgctag aagtacagaa atatcattta
     9481 aatgaaacag tctatgatga taagacagga aggatcgatc caagaaaatt gaatgctgta
     9541 agccgtttgg caggaaatga ttatgcagca ataggagaaa ttttcacgat tgcacgtccc
     9601 gaataaaaag gagcactagt tatgttaaaa ataagctata aaccatcgac agattcaaaa
     9661 gaaatgaaaa aagaatatga aacagtgaat gattttctgc aagggcagta cttagaagtt
     9721 ccgccattgc aagaccattt tgtcgttact actgttactt tagatggtaa ggaaatagaa
     9781 atgccagatc aaacgattag cggattgttt aattacttta acaaataaat atttattccg
     9841 aaaagtgaaa aaacatataa aaaaacacat aaaactaaaa aattatcaaa gaatcctggt
     9901 gcaactgaaa gaatttgcaa cgggattctt tcaacttatt tgttattttt ttctaaaaaa
     9961 tattaaagat tgttaaattt cactatttca tttcaaattc cattgcctaa tcattaggaa
    10021 aagtctatca tgaactcgta aatagaatat agtaataacc ttgttaggtg aggctcctat
    10081 acaaacatag gctgttgccc agaaacgtcg aaagtcgcca atgggtaaaa cagaaactgt
    10141 tggattaagg ctttttccaa tagctaagaa tgttttcttt acgttgtata gtgctaaaac
    10201 tcggacgatg agatgatgct aggtcagaat tcttgttcga aaaggtttct gaccttttct
    10261 ttatattttt gtgactgact tatttgatga gacaaactcg ctcctcacct gacaagtgga
    10321 ttgctgtgtg aggaggagat gcggtgaaaa aagatcgatt ggtggaggtc gctcggattc
    10381 acttctggtt gcacaataca accagaaaac acaacaagca aaactgatct ctatcatgag
    10441 agattcatat gtcgatattc caggttacgg aatgaataag ataaatgcag cttactctta
    10501 tgggggagtt gatttattaa atcaaacatt aaaggaaaat ttcaaatttg aagctcccta
    10561 ttatgcaagt atcacatttc aagattttat cgattgcgtt aatgaactgt ttcctggtgg
    10621 agtcaagatc gatgcagaaa aatctctaga tttagatggc gtatatataa acaaaggaga
    10681 gcaagtgatg gatggtaata ctttactcca gtatgctcga tttcgggaag acaaagaagg
    10741 agacttcggg aggatcagaa ggcagcagca agtgatcaaa gctatctctc agcagctgaa
    10801 agacgtaact tcaatattca agctacccaa agcggttggg aaattacttg gtagtatcca
    10861 aacgaacctt ccagaaagtg tgcttctgga ttgtggtatg gatttcctca aagacatatg
    10921 ttagaataaa caggtataaa tagtgaaata aaggaatgac aagcaagaga aggaggaaaa
    10981 aatgaaaaga agtgacaagc acggcaaaaa tcgaacaggc gcttatattg ccggcgcagt
    11041 gatcttaata gcagctgcgg gtggcggtta tttttactac cagcactatc aagaaaccca
    11101 agcagtagaa gctggagaaa agacagttga gcaatttgtc caagctttga acaagggaga
    11161 ttataacaaa gctgcagaaa tgacatcaaa aaaggcggca aataaaagtg cattatctga
    11221 aaaagagatc ttagataaat accagaatat atacggtgct gctgatgtca aaggacttca
    11281 gatatcgaat ctaaaagtag ataaaaaaga cgattctact tacagttttt catataaagc
    11341 aaagatgaat acctcattag gtgaattgaa agatctttct tataaaggaa cattagacag
    11401 aaatgatggt caaaccacga tcaactggca acccaacttg gtttttccag aaatggaagg
    11461 aaatgacaaa gtaagcctga ccacacaaga agcagcaaga gggaatatta tagatcgaaa
    11521 tggtgaacca ttagcaacaa caggcaaact aaaacaatta ggagtcgttc caagtaaact
    11581 tggagatgga ggcgaaaaaa cagccaatat caaagcgatt gcttcctcct tcgacttaac
    11641 agaagatgct atcaatcagg cgatttcaca aagctgggta caacccgatt actttgtccc
    11701 attgaaaatc attgatggag caacgccaga acttccagct ggagctacca ttcaagaagt
    11761 agacggcaga tattatcctt tgggtgaagc agctgctcag ctgattggtt acgtgggaga
    11821 tatcacagca gaagatattg ataagaatcc agaattaagc agtaatggaa aaatcggtcg
    11881 ctcaggtttg gagatggctt ttgataagga tcttcgtggg acaacaggtg ggaaattaag
    11941 cattacagat gcagacggtg tagagaaaaa agttctgatc gaacatgaag ttcaaaatgg
    12001 caaagatatc aaattgacaa tcgatgcgaa ggcacaaaaa atagcttttg acagtctagg
    12061 aggaaaagct gcatcaactg ttgcgacaac gccaaaaacc ggtgatcttc ttgcgcttgc
    12121 tagctctcca agctatgatc caaacaaaat gacaaacggg atctcacaag aagattacaa
    12181 agcttatgaa gaaaatcctg aacatccatt catcagccga tttgcgacag gttatgctcc
    12241 tggatctacg tttaaaatga ttacagcagc aatcggtctc gacaacggca ctatcgatcc
    12301 aaatgaagtg ttgacgatca acgggcttaa atggcaaaaa gacagttctt ggggatcttt
    12361 taaaacaacg cgtgtcagta gtgatgtatc acaagtagac ttaaaaactg ctttgatcta
    12421 ttccgataat atatatgcgg cacaagaaac gttgaaaatg ggtgagaaaa aatttcgtac
    12481 aggcttagat aaattcattt ttggtgaaga ccttgatttg ccaattagta tgaatccagc
    12541 acaaatttct aatgaagata gctttaactc agatatcttg ctagctgata ctggatatgg
    12601 acagggcgaa cttctaatta ctcctatcca gcaagcagca atgtattctg tttttatcaa
    12661 caatggcaca cttgtctatc ctaaattgat tgcagataaa gagacaaaag ataagaagaa
    12721 tgtcatcagc gaaacagcag tacaaacgat cgtgccagat ctgagagaag ttgtgcaaga
    12781 tgtaaatggt acagcacatt ctctttctgc tttagggatt ccgttggcag cgaaaactgg
    12841 tacagcggaa atcaaagaaa aacaggatgt aaaggggcaa gagaacagtt tcttgtttgc
    12901 tttcaatcct gataaccaag gttatatgat ggtcagcatg ttggaaaata aagaagatga
    12961 tgattcagca accaaacgag catcggaact attgcaatac ctcaaccaaa attatcaata
    13021 aaaaattgct ttttaagtac agtaaaagga ctgtggcgtt atcgtcacag tccttttttc
    13081 aaataaacgg ttttcacgat gtctctttca caacttcttt ttgatttact cacacttcat
    13141 aaactttttt tgtaaaatat ttgagaacaa gtggagaaat aagtgtactt aataaaatca
    13201 cgataactaa aggtgaatag tactgtggtt cgatcaattg actttgctgt ccaatttgta
    13261 aaatgatcaa agccatttct ccacgcgaaa tcataccagc accgaccatc aatgaactat
    13321 gttgagaaaa accggcaatc tgagcaccaa aataacctcc aagcaatttg gtcaaaatcg
    13381 ctacaagcgt caatatagaa ataaagagta gttgttcact aaagtcagaa aaatcaactt
    13441 ctaatcctac actgacaaaa aatactggta taaatactgc atatcctaaa gcctctacat
    13501 tgtggtatac ttcttcttta acctttgtct gactgacagc tatcccagca aaaaaagcac
    13561 caatgacaga gctaagccca acaagatccg ctacgtaaga cattcctaaa caaataacta
    13621 gcgatacgat gataatagaa gaatttgcgt acactttttc tgcaaatgaa agtaggaaag
    13681 gcgcaatcca tttgactaat aagaaaataa aaacaaaata aattagctgt tcaatcaaaa
    13741 caagcggtaa agaagcattg ctagaagctt cccccgttag aaaagatagg ctgaaactta
    13801 gaactaaaac gactaaaatg tcatctacca cagaagcacc caaaattgtt gatccttctt
    13861 ttgtattcac tacgtttagt tcttttaata cttctactga aatactgaca gaagttgccg
    13921 caagaataat cccaaagaaa atcgcttcat ttcccgaaac ttggaatgct ttgcctgtca
    13981 accagccaaa aacaaccggg aagatgatcc caagcaaagc aacgaacata ccaggtcgga
    14041 aatatttttt caaaagagat aaatcacttt ccagaccagc taaaaacatt aataagatca
    14101 caccaatctc cgaaaagtca tggactagaa tatcaggttt gacccaattc aatccagctt
    14161 ggcctagcaa aacaccaaca agtaattgtc caatcactgc tgggatacct aatctccgcg
    14221 agatatgtga gccaatcgtt gttgaaatca atatcaggca taaaatacca ataaattcca
    14281 ttctcatcat tcctttgtat aaaatatagc ccgaacttag cataacatac attattgaaa
    14341 aaataggaaa atatactcac aaaaaaacca acaatccaaa agattttttt ggattgttgg
    14401 tttttatacg gtctatttta ataactaaaa atgattaacg tatagtcatt tttcttattc
    14461 tgatgaaatt gataatctcc tggatgccca tgaaaatcaa aataaagccg aatatttgga
    14521 atagaagcat caaacttttg aatggattga acaataagac gaatccgcca atcaacaaca
    14581 gaatactact ataaacactc caacttttag cttttgtatt catgctgaag aacaactgga
    14641 acaagctgtt gatgataaca gctaatccaa gaagaatcgg caggatagaa acgattgcag
    14701 gtgcaaaagc atagacgatc agagctaaga tcaggaaaaa aagaccactg aacagaccga
    14761 tactccaaga acctgttttc cttttaaaac ggtaagcctc aaaaagattc aacaacccaa
    14821 gcaaaacaag ataggcggtg atcagataac cgataaaatg aaaaacagca gaagggttta
    14881 gcacaatcgc aatcccagaa ataatataaa cagcagctct taataaggcg tatttttgaa
    14941 tattttgttt aatcgtctca aacatataag aaaacctcct ttgacttatt ttaacgtgaa
    15001 ctagtcattt taccaaatta aatgttacaa aatgagcgaa ggagaagaat atctctggaa
    15061 aaatcaactt ctgaggatct tttccatttt tttgcctttt gctagctcat cgatcatttt
    15121 atctaaataa cgaatttctt tcatcaagtt atcctcgatg tcttctactc gtacaccaca
    15181 aacaacacct gtaatcaagg aacgtgaagg attggggaca ggggcttccg caaaaaattg
    15241 ttcgaaagtc acttgttttt gtagttgata ttctagttct gtttgggaat aacccgtcag
    15301 ccaacaaatg atttcatcta cttcctcttt tatacgatct tttctctccg cttttgctac
    15361 atacatagga taaacgcttg caaaacttgt cgtaaaaata cgattttgcg ccataatcag
    15421 tcacctttct ttttttcaaa ttatttacta cttatagtat ataagaaaaa gagaagaaaa
    15481 agcgattatt caaaaaaaca aaaaggattg tgacaaaatt cttgtcacca tccttttttc
    15541 cgctctctga ctttttacaa tcttcatagc tcaagtgcgc atcagataat cgaatgctcc
    15601 taaagctgca gtagctcctg aacccattga aacaatgatt tgtttgtatg gactatctgt
    15661 acaatcacca gctgcgaaaa cgccggggat attcgttgcg ccatttttct cggtcactat
    15721 ttcatttctt ggattcattt cgatcgttct atctagccaa tccgtattag ggagtaagcc
    15781 gactagaatg aatactcctg aaacagctag tgtatgcatc tccatcgtac ggcgatcgat
    15841 ataggagaga gattctacct gattcgtacc gctgatttct ttcgtcgctg catgtttgat
    15901 aacagtgaca ttcgcaagag agactagttt atcctgtaag atttgatctg cttttaattc
    15961 tggtaagaat tctagaacat atacatgttt tgctaaccca gccaaatcga ttgctgcttc
    16021 aacaccagaa ttaccaccac cgataacagc aatctctttt cctttaaata aagggccgtc
    16081 acagtgtggg caataagcaa ttcctttatt tttaaactct ttttcgcctg gtacattgat
    16141 agaacgccag tgcgcacctg tcgcgatgat ggcggttttt gctttaagtg atataccgtt
    16201 agctaagcca atattaataa aatgttgctc tttatttaag gaaacggcct gttgattcat
    16261 cataatgtcg atagggtaac tacgcacatg actttctact tgctgcatca attttggacc
    16321 ttctgtgtaa gaggtaccaa tcatattttc aatccctagt gtttcaacga cttgtccgcc
    16381 gaaagaatca gcgacaagtc ctgtacggat tcctttacgt gctgcataga tagctgcact
    16441 actagctgct ggtccaccgc caatcactaa tacatcataa ggttcttttt caaacaactc
    16501 ttcttcaatc aaagggccag tgattttttc aacaatttgt tcgatcgtca tgcgtccgct
    16561 actaaattct tctccattta aaaacacagt tggcactgcc atgatttttt tatcatcgat
    16621 ttctttttgg aacatcccac cttcaatcat cgtatgagta atatttggat ttaatacagc
    16681 taatatattc aatgcctgta caacgtcggg gcaattatga caagtcaagc taacataact
    16741 gtcaaaatgc aatgtttgtg ttattttttt gattttaaga ctcaaagatt cactgatttt
    16801 cggtgcccgt ccactaattt gtaacatagc cagtaaaaaa gaggaaaatt catgaccgag
    16861 ggggatacca gcaaaaacga ttccactttc tttgccaact ggattgatag aaaaactagg
    16921 tgtgcgcatc gaatctcttt tttcccaaga gattttagga gacatttctg ctatttcctt
    16981 cgtaaattca gctagttttt gactattttc actattatcc aaactaacag aaaaaacaat
    17041 aggagattca atcagtgcta aatattgttc gagattttgt ttagtttctt tgtctaacat
    17101 atccaccacc taaatctttc caacaagatc aaaacttggt ttcaatgttt cgccactttc
    17161 tttccatttt gccggacaaa cttcacctgg gtgggtacgt acatattgag cagcgcgaat
    17221 cttatcaatc agtgcactag cgtcacgacc gattccatct gcattgattt ccatagcttg
    17281 aacgattccg tctggatcaa taataaatgt tcctcgctga gctaatcctt gctcttcatc
    17341 caaaacatca aaaatacgag aaatctgatg agacggatca ccgatcatca cgtattcgat
    17401 tttaccgata gcatctgatg tatcatgcca tgctttgtga gtaaagtggg tatcggtaga
    17461 gcaagaaaag acttcaacac ctaaagattt caatgtactg tattgttctt gtaagtcttc
    17521 taattcagta ggacaaacaa aagtaaaatc agctggatag aagcaaagaa tactccagtt
    17581 ccccataaga tctttttcac tgacttctaa aaattctcct tgatgataag catgtgcatt
    17641 aaattcttcg atttttttcc caatcaatga catagtaaaa cctccaataa taattattta
    17701 tttataatta ttgtaattta gttggagttg caaagtcaat ggatattttg ttatttaata
    17761 aagattactc aaaaacgaag gttaattata aaaatagaag aggtcgcaag aaaatgctac
    17821 tgctggaaat actcgggata acgttgagta acagtttcct gttctttcag catcatgtgt
    17881 aagtgcaatc ccgtttgaaa tcgagcttct tctggttttt tatcaactaa agccttgagt
    17941 aaatcataat gttgttccat gatcgtttcc catggaagcc cctgaatggt cactcgtaac
    18001 cagcggaaac gttctaaatg ggtattatgg tcatctaacc agttccaaac ttcttttctt
    18061 cctgcaattt cgaaacaagc ttcatgaaat aaattatctg cacggaaaaa agcgcgttcg
    18121 tcatttattt tgactgcttt ttcttgttct tctaaaatcg tttctagcac tattcgtgtt
    18181 tgtgtgtcca atttggaaca acattcaatc atgatttgct cttccaactg ttctcggaca
    18241 aagcgagcat tttctgcaga aacaagatta attttggaaa catacgtacc actttgaggt
    18301 atcgtataca cgagttcttg ctgacgtagt tgaatcaatg cttcgcgaat gggagtccgg
    18361 ccgatagcaa gatgttcctc cagtgccttt tcagatattt tccttccagg ttctaagtca
    18421 gcatagataa tttgtttacg aatcgcttga taagcttgtg attgcaagct gcttttcatc
    18481 tttattcctc caaaatcatt tctatgaacg tagtataaca gaagaggtct gaaaaacaat
    18541 ctggtatgtc acattaacta gtaaatttgt ataaatagta aaattttggt tgacatagcg
    18601 atttttattg cataaaattc acttgaatac aaaaactaat atgttagttt aggaggggaa
    18661 aaatgattga tttaaacaac tggaggaact ttatccctga attatctcaa aaagaagtag
    18721 aaatacctca ttttgaccgt gcatcattaa gagaaaatgg acaaaaaaat cctgtctgga
    18781 tccatttcgg tggcggaaat ctttatcgag gatttcatgc agaaatcgct caacgtttag
    18841 cggatctagg cgaattgtcc actggtgtca ttgtttgtga aacattcgat gaagaaataa
    18901 ttgaaagggc ttaccaagat tttgatgatt tgattcttga agtggtcatg ccaagcagtg
    18961 gagagttcgt caaacgtata ttagctgcga cagctgacag tctgtattgt catccagatt
    19021 gttctgaaca ctttgataag atgattcgac actttgaaaa tcctagttta caattagtta
    19081 cttttgctat tactgaaaaa ggttatcgat tgaaagatac aaataataaa tatataggac
    19141 cgattgcagt cgatataaaa aatggtccaa agaagccatc acatacaatg agtattcttg
    19201 ctgtgctact ttataaacgt tttttatctg gagcttggcc gattgcactt gtctctacag
    19261 ataatttttc acaaaacggt agtcaattac gtacttcttt gctaacgatc gcaaaagaat
    19321 ggcaaaagga aggttatgta caaaatacat ttatccaata cttatcgaat gaagaaattg
    19381 ttgcgtttcc atggacgatg attgatcgta tcaccccgaa tccttccttg tcagttagca
    19441 aaatgttggc aaaagagggc ttttcaaata tgtcgatttt ccaaaccgca aaaggaaata
    19501 atattgctcc atttgtcaat acagaggaca tccattattt aatcatggaa gatcattttc
    19561 ccaatggacg tccgaaattc gaaaaagctg gggtattctt gactgatcgt gcaacggtag
    19621 ataaagcaga tacgatgaag gtgacgactt gccttaatcc tttgcatacg tctttagcta
    19681 ttatgggttg tttactgggg tataggagca tagctgagga gatgaaagat ccagatttat
    19741 cgctgctagt ttctgaaatc gggtacaatg aaggcctccc agttgtggag tctccaggta
    19801 tcattgatcc aaagatcttt ctggaagaag taattactgt gcgcttttct aatccaaata
    19861 ttcctaatat gcctcagcga atcgccacgg atacctctca aaaggtggcg attcgttatg
    19921 gtgagacgat taaaaaatat atggaaatag aagttaaaga cccgcagaca cttgtattca
    19981 taccattgac tctcgctgct tggcttcgat accttattgg cattgatgat caaggaaaac
    20041 cattcatccc gagtccagac ccactattga cagaccttca gcataagctt tccgatattc
    20101 aactgggtac gcaagataca tcacatattc atgagaaaat ggctcctatt ttgtcaaata
    20161 aagaaatttt tggtgtagat ctttaccaag cagagcttgg cagaaaaata gaagaaatgt
    20221 tcatcaagat ggtagcagga ccaaatgccg tacgaaaaac attacaggat tacttacaag
    20281 aacatggagg aaatttatca tggaaatgac ttttagatgg tacggatcac aggaagaaaa
    20341 gattacctta gatgaaatca gacagattcc tgcggtcaaa gggattgtag gcacattgat
    20401 ggatatacca gtaggagagg tatggcctag ggagaggatc aaagaactaa aagaagaaat
    20461 tgagtctcgt gggttaacac taaaagtaat tgaaagcgtc aatgtgcatg atgccattaa
    20521 aattggatta ccttcccgtg agcagtatat cgaaaactat aagcagacaa tccgaaactt
    20581 ggcagaatat ggaatagaag tcatctgcta taattttatg cctgtatttg actgggtgaa
    20641 atctgacctt gactaccgct tagaagacgg ctcatccacg ttagctttta tatctgatga
    20701 tattccagat gatccaagag aaatcgtcgc gcgcatcgaa cattccagca atgaatttgg
    20761 attgccggga tgggaacctg agcgcttagc tcctatcaaa tccttgcttg aagcctatgc
    20821 atcagtagac gaagaaaaac ttcgagaaaa ttttgcctat tttctccaat caatcatccc
    20881 aacatgtgaa gaggtagggg tcaaaatggc tgtacatcca gatgatccgc catactcgat
    20941 ttttggatta gctcgaatcg taaagaatcg agaagattta gattggttgt gtcgcatagt
    21001 agacagccct tataacggaa tcactctttg tacagggagt attgcagaag atccagaaaa
    21061 tgatgtatat gggattttag cagaattttg ccaaagagat cgaatcccat ttgcacatgt
    21121 tcgaaatatt aaatttttag ctaatcgaga tttctatgag gcaccacata aatccgaata
    21181 cggttcatta gacatgtacc gcatactaaa aacaatgtat gacaacggat ttaacggata
    21241 catccgtcct gatcatggaa gaatgatctg gaatgagaca ggaagaccag gatacggctt
    21301 atatgatcga gcattaggtg ctgtttatct aaatggatta tgggaagcaa tcgaaaaagg
    21361 aaacaaaagt aaagataatt agtgaaaaaa actgtaattt tattatgtaa ataagaagga
    21421 tgttcaaatt cttgatacat aaatccggag gttgttaata agcattcgga tttatgtatc
    21481 ttatttggtc taggaagtaa cttgattttc ttttttcgat tgatccatga gtgcttcttc
    21541 ttttaattca aagatatatt ctgagatcct cataatcact tttttccagt cttctggtgt
    21601 gatgattcct tttaaaatat agattttttt gatatgagaa ggcaattgtt tcaaagggca
    21661 gtcgctgata acgatgttgc agttctccat aaaatcattt tgtattttga tcgattcatt
    21721 tgaaaaaatt tgttctatct taacgatcag atctttactg atataaaaat cacgcgtata
    21781 gttcacacca atcacaattc gcggctgttt tttgtagcga tcatacaaat gtactaggtc
    21841 ttcaaccatc cattgcacat tttccttttc taaaataact ggtaacaagt gttgattttg
    21901 ttctctgaaa tccatataaa attgttcaat ggcggtttgg acttctttca atacgctatg
    21961 ttcttgataa agcatgaaag taggaagggg cgtatttggt tgattgaagt taagaaatcc
    22021 attataaagt ttatttcgtg cgaatgccaa tgagtaaaga atcttttctt tttcaggtat
    22081 aaacaaatca aatacagtac aaagggactc caccatttct cttgaataaa gaacatgagg
    22141 aacgtttgct ttttttaaaa gtttgtattg ctccaagctt gtttcttccg attcgatttt
    22201 cgtgatagaa agtcgagcta aaatattcac taagatgcgt tgttcgcgac aaattggcag
    22261 attaggtcgc aaaatatcta cagagagatc aataaaaaag tcaatatatt catactctgt
    22321 gaattctttt tgtacgtctt tcacagaagt tgcaggaaaa ttctctttac atatttttaa
    22381 tagaaaatat aatttatcta tcataccgct atttagtcgg tcaagtactt cttttttaaa
    22441 aaaagtattc atcataaaag ccactacttg cggatcttca tcataaaatt ctgtagcagt
    22501 acttgaaaag atgttccaat aaatatctaa tgctgcatat tgaatttgta cctcttttcc
    22561 agaaaaagac tttctgccat tattagaaaa ggagatagac aaaccgtact atgctaaaaa
    22621 tgcattaatc tttttcattt tcgtatacaa ataagaggca ctatagtttg tttgctgact
    22681 gaaactgata atcgaatttg tctgacagct gaacaaagaa gtcaagatct gataactgac
    22741 tgattcgttg cagtatcttc caaatagacg ataatagcag taagccggat caattccttc
    22801 aaaattcaac tttaaaaaag gtttttgctt atcaatatgc agttctgtta gattgagacg
    22861 ctcaaagtct tgttctaatt ctttgatgta tagtaaaaaa tttgattcag acatatggaa
    22921 atgatctgct aaatattgtg tagtaataaa gaaacgatgt tcagatagga aaagaaaaat
    22981 atctaatttt gtattgcttt ttttcttcaa gataaacgtt ttaatcgctt cttgagtttg
    23041 catgaaagag gctccttttt acttaaattg ttaaaacttg aaaatattac tgtttttttc
    23101 ataaatttag acaaaaaaac agtaaacata taggtagaat ctctactatc ttatttcttg
    23161 catacaaaaa aatcaaggag tgagagagtt atgagatatt caaccatctt aatatcaaca
    23221 tttgcagcct taagcttatc gcttactata aatcagacag tccacacaga ggagacagaa
    23281 acagccaaat ggacagaaac caagtgggcc aacgtcaatc gaattacctt tcaggatgaa
    23341 ttttcatcct tcaattcagg agtcgcaaaa ttaagaaaag gacaaacagc gaccatcaaa
    23401 gcaaaaattg actattcggg agacttagca cctatactac aatcagctgt tacatttgag
    23461 aatgttgatc ccactttgtc gattggctat gacgagaaca ctttgactat ccaacacaag
    23521 caagcgatat ttaccttcac cgttacatta aatcaggact tgttaaaacc agcgcttttt
    23581 actgtcaagg tatcagatgg actaccgaac gatcatcatt taacgccgta tagtcagcgg
    23641 cagacaattg agcaagattc agccatcgac tcaggaggag atcttgttga agaaccaaca
    23701 gataaaccag aaaatgaaaa taagccagaa gtaccgccta cagaaaatcc agacggagaa
    23761 cagaaaccgg aaatagaacc tggagaagaa cccgatacag aaactcaacc agagccagac
    23821 aataaatcaa aacctgaaat aacccctgga gaaaaaccag atgtaactcc agaaccagat
    23881 acggattctg gaaaccaaac agtaccggaa acaaatccag acacagataa tgaaactgaa
    23941 aatccagaaa aaccagaggt agatccagaa gaaaaaccag atgtgactcc agaaccagat
    24001 acggacgcaa gagaccaagg aataccagaa aaaataaaca aaaaaacaat tcaagaggat
    24061 ggaaaaaaag agagtaaaaa atcaaatcta gcaattctaa aaataaatga agagcagctg
    24121 aataaaaaat cacgaatatt tgatagcgcc caatcagctg aaaccttaaa aagtagcaag
    24181 gatacaacct ttgctgtcct gaaacgaaaa ataaacagct accaaaatcc ggagagtcac
    24241 aaaacaaagt tatactgtgg agcggaatca ttctattaag tatagcaaca atgctttctg
    24301 caaaaagatt taaacaaaac agatctctct aacatttgaa taatgtctgt gaacgcattt
    24361 ttcaaataaa aagaaatatc cagaaaatga ccccaaaata ccagattgaa atctgatatt
    24421 tggggtcgtt catactttat ctttcttcat atttatttta agggatatct gtttcttttg
    24481 gaagatggtt cgggatcttt ttcactttca atacatctaa aaagaaggta aagatcggaa
    24541 tgccgacaat caagccccaa acgccaaaga accgttcgct aacaagtaag accacaaatg
    24601 tataaaaaat cggcagttct gttttgctgg acatcaactt cggattcagc acataagact
    24661 caattagatg gacaatcaaa atcgtaatca ggatataaac aacatctcgg atgcctcctt
    24721 gggaataagc tatgaaagat agaggaatac aagaaatgat aacacctgct actgggatca
    24781 gacttaaaat gaaaatcatg atcgctaaac tcagcagctg atgaaagcca aatacagcca
    24841 aggcgattgt agtgagcaac gtatttaaaa gagcgacaac aaattgagct tctaatacca
    24901 aaccaaatgt attgacaaac ttatcggcaa aatagtaaat atcttgaaag aaccaagaaa
    24961 attcactttt taaaaacaat cttgagaata caattgtttt tttcttttca atcataaaaa
    25021 agaaacttaa gatgaatgac atagcaaacg ccactgccat ttttcctata tcctgtaaat
    25081 agccaagcgc aatcgaagcg ccattttgaa gctgttcaaa aaaatcgttt cgctcaatat
    25141 aagtatggat atattgtaaa acaggatctg catcttttgg ctgatgctcg taaaaatcaa
    25201 taacggactg gatcatatca aatgtttgat gaataagcat cgggatatac tttgtgaatg
    25261 ccagatagat aaggtaaacg atcagtgtat aaatcatgat agctaaaaca cctgtaggga
    25321 ttcggacaaa tcgctgaatg atttgaacaa gatgaatcat caaataagta aagataaatg
    25381 tgagcaaaat ggttgtaatc atacttctaa ctaagaaaag aacaaaaacg acaaataata
    25441 aaacagtgag acggcgtatt ttttcattcg ccaaaaaacg ctgataaacg gtcaaatcga
    25501 caacctccaa ataacaaaaa ataaacacgt tattcattat atgatggaat atgaaaataa
    25561 gaaagagaaa gagcaaaaag aaaaaccgct aaatgacgtt atcttatgag ttggttttcc
    25621 tcataaaatc gttcatctag cagtttcagc cctttttctg gtcttgatga aaattatcta
    25681 gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc ctgttcaagt ggaagataag
    25741 acttgatctt ttgtggaata ccttctgctt gtttttgcaa cgcagcacct tgaggagtca
    25801 gcgtaacatt tacgattctt tcatcacccg tttcacgctg acgattaata taattttttt
    25861 ctgctaactt ttttagtaag ggagtcagcg taccagaatc cagacacaaa tcatttccta
    25921 gctctttcac tgaagtcttt ttcttttccc ataaaactaa catcacaaga tactgggtat
    25981 aggtaagatt cagcggcttg aggagggaag tgtagtgtct aatgatttcc ttagaggcgg
    26041 catataaagg aaaacatact tgattaacta aatatagatc ttccattttt ccacctccgc
    26101 tgttttacta tagtgtatca tagcggattt acacaaaaaa gtctattcac taactgcttt
    26161 cagagtaact tctatatttc cagctgtagc ttttgaatac ggacacactt tatgcgctgc
    26221 ctctaataat tcttgcgttt cttccagaga aagaccttcg atagatcctt caatctctac
    26281 acctaagaca aagctcgttt cactttctga ataaagggaa acttgtgcac tgacagtaga
    26341 agccccagag atattttttt gctgtttgat caattctaat gcactgttaa aacatgcact
    26401 gaaaccagct gcaaacaatt gttcagggtt ggttgcattt tcgacgcgtt taccaggagc
    26461 tgcaatattc aattcgaatg tacgatcggg attatgaact tctccagagc gtccgcctgt
    26521 gttgatcatt ttcgtagaat acattttttt cataaaatta ccacctttaa ttatatttta
    26581 tacaattgaa ttttacacaa tataattgtg gaaagcaaag aaatggattg gcataagata
    26641 aataagagaa agcacatgaa acagacgcgc tttctcgaat agcaaacata tacaaaaaaa
    26701 tatcagagac aaaaactaat ctcatcataa acattacata tatttctcaa aaagttgttt
    26761 tctttcttca aaacttactt cacgatggac ctgatgaagc atccataaat aaccaaaagg
    26821 atctctgaac attgagttag tcacaccaaa gtccatcatt tctgtcattt cttgaatctc
    26881 tacacagcca tttcgtaatg ctttattatg tgtgtcctcg atatcagaga cgatgatatt
    26941 gaaccagatc gtatttggat gttcagcagt aggagcagtc aaaccaagag acgtgttttc
    27001 atccagcata tggaatctcg tgccatacaa ggtaaaaatg acttcattat tgcctctagg
    27061 gtaatctgtt gcctcaatca gttgtatttc atcaaagatt gtttgataca gttctagtgc
    27121 tttcaaacta tcatttaata caaaatctat ttccgttcct ttatgcataa agatctcctc
    27181 tccttttcag tcgaacagtt actagattct tttattatac cagttaaaga aaacgcattc
    27241 aaaaaaatag cggatttact taccaactca ttcgattgga tgataagtaa atccgcgtcc
    27301 cattacttct gctacgctgc tgacaatgac aaaggcactt ggatcgattt cttctatcaa
    27361 tggtttcatt cgggaaaatt catgatcagt cacaacgatc aataaaacct cttttgactt
    27421 tcgattatac ccgccttttg cctctagcag ggtaaggcca cgtccaatct cagcacttaa
    27481 gcgaaggcgg atgtcttcca aataatgttc actcatgacc ataacagatt tctttcgatt
    27541 gatacctgtt tcgatataag tcatgacaat tgaagtgatt gcaatagata aaatagcata
    27601 aagaaactct tctattccaa aaacaaacag attaagagaa acaacgaatg cgtctgtaaa
    27661 aaataagcca atcgaagtat tcaagtggaa atatttttta aaaatgaggg gagggatcgt
    27721 cgtaccgccg ctagaagagt tgtttttata taaaatcgca actcctaaag caaaaatcgc
    27781 acttccaaaa atgacagaca acaaccgatc tgaagtaatc attgtttctg gaactattgc
    27841 aatgattaaa ggaaaaaaca agcttccgaa aagtacttta aaaaagggtt ttttacctaa
    27901 aaatagaaga gctagtacca acataacgat attcaacact aaaataacta tagctcggtc
    27961 aaatcctaaa acagcttcca gtaatattcc tagaccgctg acacctccag cagcaatatg
    28021 atgaggccct aaaaacatat tgatagcgac tcctaatatg aagagggcaa aaataatctt
    28081 aatgtatgtc catataaatt gtctcgtttc tttcatttat ccacttcctt aattcattaa
    28141 taatactctt atcttattat aacttacttt tcaccttaag ttaactttaa ttaataaaaa
    28201 aattaggcta gtctaaatta ttatgtttca ttcttctaga atatacttct acgttaaaaa
    28261 ctgttaaaaa acttttactt tagaaaattg gagaaattga tcgcttaaga taaattcagg
    28321 acactaaata gtacaggaag tagattaaaa cttttaaaag ttttaagtct atctatttgg
    28381 caagtttaaa aaataaaaat tcggtatcaa aaaactgtta ccgataacat ttttgaaagc
    28441 gttgtctttt gtttctctat cacttatagt gatgacgtca tattaaaaca caaggaggaa
    28501 atcaatggat acagctgcaa tttatcatcg accggaaagt gaatatgcat ttctatatac
    28561 gaatacaaga tttcgtattc ggcttcgtac taggaaaaat gatatcaaaa aagtatatgt
    28621 gctttgtgga gatccatata ccataacaac tgaaaaatgg tatcaaaaac aacgaccaat
    28681 gaaaaaatgt ctaagtacca atgttcatga ttattgggaa attgaagtga cgagcgaaac
    28741 aaggcgctta cagtatgcgt tccatgttgt tggggaagat aacacggact gtttttacgg
    28801 tgatcaagga atttttcctt atcgagaaaa tgtgattact gaaccaaatt tttattttcg
    28861 aataccttat tttcatcaaa tcgatcgatt cactattcca gaatgggtaa aagaaacagt
    28921 atggtatcaa atttttcccg aaagatttgc aaacggggac aaagcaaatg accccgaaca
    28981 cactttacct tggggaagta aagatccaga ccgagaagac ttcttcgggg gagatttgca
    29041 aggtgtgagg gatcatttgg attacttgac ggaccttggc gtcaatggca tctatttttg
    29101 tcccatattt aaagccacat ccaatcataa atacgatacg attgattatt atgaggtgga
    29161 tcccgctttt ggcgataaaa aacttttaaa aaacttgatt gatgaagcac ataaaagagg
    29221 gatacgtatc atgttcgatg ctgtattcaa tcatatgggt gtccattcac cacagtggca
    29281 agacgttctc aaaaacggag aaaaatcgat ctataaagac tggttccaca tccgtttttt
    29341 cccagtagat tcttatcaga tgactgactt accagaaact gctgaaaata tcccatatga
    29401 tacttttgct tttactccat tcatgccgaa actaaataca gcaaatccag aagtgcaaaa
    29461 atatttgctg gatatcgcaa cttattggat aaaagaattc gatattgatg gatggcgtct
    29521 tgatgttgct aatgaagtcg atcatcattt ttggaaaaaa ttcagagaag cagttacaga
    29581 aatcaaacca gacatctata tccttggaga gatttggcat tcctctcaag cttggctgca
    29641 aggggatgaa ttccatgcag tgatgaatta tgcattcaca gattcgatta aagattattt
    29701 tgcaaaaaag aaaatcacag cttcacaaat ggtcagtgga atgaatcatc agcaaatgct
    29761 ttacagagat caggtgaacg aaggaacatt caacttatta gattctcatg atactgccag
    29821 gatcttgacc ctttgccaag gtaacaaaga attgatgaag tctgtgatgg cctttatgtt
    29881 tcttcaaaaa gggtctcctt gtatctatta cgggacagaa atagggatga ctggtgaaga
    29941 tgacccggat tgccgcaaat gtatgatatg ggagaaggag gaacaagatc tagagctatt
    30001 cggttttata aaagaacttg tttctctaag gaaacagttg tctaagatta tttctgaagg
    30061 ttctactcag tggctaatag tatatgatcg tgaagataag ctctatttca cacgggaact
    30121 agagggacag atcatctatg tttactttaa tcagtcaaaa gaaccttggg tagtagaaca
    30181 ggaaaatgag gtgattctta gtcaaaattg tcagttgtta gaagacggga aagcagagat
    30241 acgacaatac ggatttttga tcatgtgttg aaaactgtta ccggtaacaa caagaaagcg
    30301 tttgcaaatg aagatttgtg cccattataa taagggtgta acagcacgtt gaatacacag
    30361 gaggaaatga agatgaaaaa aagaagtgtg aaacagttgg gtttagggtt aatctcttta
    30421 ggaatcttag gcggattagc ggcttgtggc aatggtaact cttcagcaga aggggaagcc
    30481 gatgaaggaa aaacgttaac agtttccgtt gattctggat ataaagatta tataaacgaa
    30541 ataaaaaaca ctttcgaaaa agaaaacgat gtgaaaatca agctagtaga aaaggacatg
    30601 ttcgatcagt tggaatcatt agcgttagat gggcctgctg gaaagggacc tgacgttatg
    30661 atggcagcat atgaccggat cggtgcgtta ggacagcaag gacatctggc ggaagtaaaa
    30721 ctgggaaatg aagcagctta cgatgaaaca gacaaagccc aagtgactta tgatggaaaa
    30781 atctacggag aacctgcagt aatcgagacc cttgttttgt actataacaa agatttagtc
    30841 gatacagcac catcaacgtt taaagatttg gagaatctat ccaaagacag tcgttttgct
    30901 tttgagtcag aagcaggaaa aaatacagga tttttagcta aatggactga cttttattat
    30961 tcctatggtt taattgcagg ttacggtggc tatgtatttg gtgatgatgg cacaaatcct
    31021 tcagatatcg gtttgaataa tgcaggtgct gtagaaggaa tctcttatgc aacagattgg
    31081 ttcaaaaatg tttggccaaa gggaatgcaa gacataaaaa gtgcaggaga cttcgccagc
    31141 cagcaattca tgtccaataa aactgcagca atcattgacg gtccatggca agcgcaaaca
    31201 tacaaagaaa acaatatcaa ttatggtgta gccaaaattc ctactctaaa taatggacag
    31261 ccttatcaac cgtttggcgg aggaaaagga tgggtagtca gcaattatgc taaaaacaaa
    31321 gacctctctc aaaaatggct tgattatgta acgaatcagg aaaatcaaga aaaattcttt
    31381 gaaatggtaa atgaaatccc tgcaaaccaa caagctaggg aaacagcaaa ggctaaaaac
    31441 gatgaattaa caacagctgt gattgaacaa tatgaaacag ctcaagcgat gccaaacatc
    31501 ccagaaatgg gcgaagtttg gacaggtgca gaaaacttga tgttcgatgc agcatcaggt
    31561 tcaaaaacac caaaagaatc cgcagatgaa gcagtaaaaa caatcagtga agccatagaa
    31621 caaaaatact gattaaaatg ataaccgtcc atttttataa aaaatggaac aggtgcttca
    31681 gataaaagtg gcaagagcga gatggaaaat tatcttgctc ttgttgccat tatcttaaaa
    31741 gcagtttgcg gtttagcaag tttagagggg gcattcagat gcagcaagat aaaacaagca
    31801 atatcaacga aaaaaaaatc acgctttgtt cacttattcc agggatgggg cagtttgtaa
    31861 atggccaagc ttttaaaggt atcgtttttt tcgccatatt cgtcctattc attggtcaga
    31921 tgcttctggg cggatttcat gcattggtag gattggttac tttgggaagt ataccaatgg
    31981 aagatcattc attatttatt ttgattgaag gcactttgca attcatcatt acaatcattt
    32041 tcttgatttt ttacggtttg aatcttttag acgcttatcg agtagcgaag atgcgtcaag
    32101 agggcaaaaa aatcaatcgg acgatgaagg aagtcataca taatataggt gatgaaggtt
    32161 ttccttatct attgacactt cccgcttatc ttctcatgat ttttacgatc attttcccag
    32221 tcctagttac attgttcact gcatttacta attatgactt caatcatatt ccaccagcca
    32281 gtttaattga ttgggtaggg gctaaaaact tttttagtat cttcttttta agctcatatc
    32341 gtaatacttt ttttgcagtc ttttcgtgga ccgtgatttg gactttatgt gcaacaactt
    32401 tgcaaatcac ccttggtatt ctcacagcgg ttgttgctca ccagaaattt attaaattca
    32461 aacgtatttt cggtatcatt ttcttattac catgggcggt cccagctttt attactatca
    32521 tgagtttttc gaatatattc aacgatagcg taggtgccat caatactcaa gtgattcctt
    32581 tcataaacca gcttcctttt gtagatatca gtgcaatctc ttggaaaacg gacccgaact
    32641 ggacgaaagt agcaattatc ttagtgcagg gctggttagg atttccctat atctatgtga
    32701 tggttacagg aatcttacaa tcgatttcag aagacttata tgaagcagca aaaatcgacg
    32761 gagcaaatgc atggcagcgc ttttcaaaca tcacgcttcc tgctgtcttc ctagtggcag
    32821 cgccaacatt tattacacag tatacgggga atttcaataa tttctccatg atctatctgt
    32881 ttaacaatgg tggtcccgga agcgtaggcg gaggagctgg ttctactgat attcttattt
    32941 cctggatcta taaacttacg actggaactt ctccgcagta ttctatggct tcagcaatca
    33001 cgttgattat ctccgtcatc gttatctgtg tgtcattgct ggtatttaaa aagacaaatg
    33061 cgttcaatat gggggattaa caatgaaaaa atttcattcg agtgctcgat tccaaaaacg
    33121 actttctcac ttttttactt acttttttct tttaatactg tcagtcatta tcatctatcc
    33181 gcttttgatc acagcaagta ctgccttcaa gtcaggaaat attgcagctt ttagtttaga
    33241 tctttcaagt gactggactt taggaaactt tactcggcta ttcagtgaaa cactttacgg
    33301 gacttggtat aaaaatacat tgatcatttc tgtcctaaca atgatcgtac aagtgactgt
    33361 agtaactttg gcgggctata cttatagtcg ctatcgtttt atcggccgca aatcaagttt
    33421 gatgttcttt ttagtgattc agatggtacc gactatggca gcattgactg ccttttatgt
    33481 catggctttc ttattagaag cgctcgataa gtactggttc ttgacaatga tctatatagg
    33541 cggtggaatt ccaatgaata cttggctgat gaaaggatac tttgacactg taccaataga
    33601 tttagatgaa tctgctaaac ttgatggggc agggcatttc aggatttttt tccagattgt
    33661 attaccgctt gtcaaaccga tgctcgccgt tcaagcatta tgggctttta tgggaccttt
    33721 tggggatttc atgttagcta agttcctgct tcgaacacct gaaaatcaga ctgttgctgt
    33781 cggcttacaa acatttattt ctgatgtgac aaaccaaaga gtctctcttt ttgctgctgg
    33841 ggcaattttg attgcactac cgatttgtct attattcttc tttttacaaa agaactttgt
    33901 taccggcctg acatctggcg gaacaaaagg ataggagcag agtatcatgc aaacatttcc
    33961 gttaaactat ttttccgcat tacgtacgcc aactcaagtg tttgcgggac gcaaactatt
    34021 atcatggcct aaatttttct tgatatttgt atttttagtt tccttgatgg tcatgcctgt
    34081 gactcttttt tatgccaatc agatccaagc gattccatta gaacaatttc tatccgttca
    34141 ttcgctgatc gatgaacaag gaacccaaaa attttctgaa cttgaattat ctgaaactgg
    34201 gttacaagca tcgcaacaaa cgatagctgt gacccctgaa attttggtag gtgtgtcttt
    34261 atctgagaaa caacaatccg atcatggaac ttttattgat tttgagaagg aacaatgggt
    34321 gatccaacaa aaggataaat ctggtatacg ccgctatacg atgaattata gcccatcttt
    34381 ccaaccagat tctgtacgta cgcccgaaga tttccaacga tttttagaaa gagaatttta
    34441 tgccagtaat cgtccaacaa tcattttatc ttatagttta agtcttggtc ttgtcctatt
    34501 tgtcatgacg agtctcattt tattcggggc ttcctttttc ttgtggatga caagaaaaag
    34561 ccaactttca tccattcata cattcaaaga gagtgcaaat ttgatgctga atgttatggg
    34621 aataggttcc atggcagcag cagttgtagg gtttatccac tttgacttta tcttgatgct
    34681 aagtgtccaa acaacgataa ctgtcttgct gcttttatgg gtttttgtca aaacaagatt
    34741 caaagataag cgtgtcgaat gacaagttta agcgatttat gcgacaatga atgtgaaaaa
    34801 aatgaaaaat aaagtagttt ttgaaaaatt tctgaaaaaa ttaaacttct tagttgattc
    34861 ttttcttttt tagtattaac gtaatagggt agttttttta aggaggaaaa tcatgttaga
    34921 gaaaacttta tacaatcaat tattttcacg ttctttctcg ctccctgtcg aagtaaatta
    34981 ttgggacggt acaacgaaac aatacggaga tacggacact cctccccaaa tcaaaatcaa
    35041 tatccatgaa gaaatcccaa tgaaagagat tacaagtaac gcttctttgg cactaggaga
    35101 agcctatatg gatcatcgaa tcgagatcga gggaagtatc caagcattga tcaacgatgc
    35161 ttatcagcaa gcaaacagct ttttacgagg aaaagattat ctaaaatggc tgccaaagaa
    35221 agaaaaacat acgaaacaaa aaaacaaaga agatatccat gcgcattatg acttaggtaa
    35281 cgacttttat aaactttggc tagatccaac tttgacttat tcttgtgctt atttcaatac
    35341 acctgaagat acgttggaac aagcacaaat caataaagtc catcatatct tagacaaact
    35401 ctttatcaaa gaaggcgaaa cgttgcttga tatcggttgt ggctggggaa cattgatgtt
    35461 cactgctacc aaagagtata atgtcaaggc aactgggatc acactaagtg aagaacaata
    35521 cgatttgatt accaaaagaa taaaagaaga acatttagaa gataaatgcc gtgtattatt
    35581 aatggattac cgcgaactaa aaggcgaaac gtttgatcat atcacaagtg taggtatgtt
    35641 cgaacatgta ggttctgaaa atcttgaagg ttatttcaaa gtggtaaaag atttattgaa
    35701 accaaaagga acagctttga tccatggaat cagccgacaa caaggcggtg cgacaaatgc
    35761 ttggatcaat aaatatattt tccctggcgg ttatgtccct ggtgttactg aattagttga
    35821 taatattaca aaaaacgatt tgcaactgat tgatttggaa agcttacgtc gtgactacca
    35881 attgacatta gaacattgga caaagaattt tcatcaagtc caagatcaag tcacgaaaga
    35941 aatgggtgat tccttctatc gaatgtggga tctatattta caagcttgtg cagcttcatt
    36001 tgaatcaagt aatatcgatg tgattcaata tttgcttgtt caacctggaa acaatgacat
    36061 cccaatgcat cgtgcataat tgaggcaaaa gtactgttta aagactattt aaaaagaagc
    36121 taagttttat gatgctccag aagtgaactt ttttgtctaa cttctaggat tgtctctaaa
    36181 gcttagtttc tttttcttta tattttcata ataaaagttt ggttctttaa tttttaaaca
    36241 tgacagaaaa ttttttcttc gttatacttg taatagaata ctattttata acaaagggga
    36301 tcgaatcaat gaaaaaaact gttatttata ctagtttatt tgcagctttg tttttagctg
    36361 cttgtcaatc aaataccaat caaggcacat ctgggaatac gtcttccagt agcgaacagc
    36421 gctcttcaca ggtatcggac tcctcaacaa aagcaagcac atcttcgtct agccagacaa
    36481 aaagttctag tgaagcaagt actcagtcag atatcaaagt gtctgcagct cgtgcgatcg
    36541 aattgttcca agataaatac ccagatactg caattactag tttggaatta gatagtgatt
    36601 ggggatctta cttctacaag atagaaggtg tcgatgatca aaaagaatac gaagcaagaa
    36661 ttgatgctca aaatgaaaaa atggagacag agaatccaga aactctagat cgagatgaac
    36721 aaaatggtca aaagaaaaaa gaagatggtc tggatgttga taaattgatc acgattgagg
    36781 aagcagcaaa aatcgccgtc gacaaagtag gaagcggtac ggcaacagat tgggatttgg
    36841 ataaagattt aggtactact tattgggaag tcaaagtaaa agacggcaat caaagtacat
    36901 cagtcaagat caatagccaa acaggcgaag tactgtcaac tgaacaagat gattaagaat
    36961 ttcttcggaa aacaagagtt gatttttctt ttagtcatga tacactagac aaaataactt
    37021 gtttgaagga gcgatttact tgagtttaga aacgaccttt gccaatcata tctttgccaa
    37081 tcctttgatg aatgcatctg gtgtccactg catgactacc caggaattag atgaactggc
    37141 tcattcagaa gcaggagcat ttatcactaa gagctgtacg atcaatgaac gaaaaggaaa
    37201 tcccgaacca cgctattttg atgttccttt agggagtatc aattctatgg gacttccaaa
    37261 cttaggtttt tcttattatc tagagtatgc tcttgcgtat gaaaaagtgc aggaaaacca
    37321 aaatcaaccg ttattttttt ccattgccgg gatgagtgtc caggaaaact tggaaatgct
    37381 tgagaaaata gaaaaaagtg ggtttaatgg gatcactgaa ttaaatttat cttgtcccaa
    37441 tgtgcctgga aaaccacaac tagcttacga ttttgaggcc acttatgaaa cactgaaaga
    37501 agtattcagc atcttttcta agccgctagg aatcaaattg cctccttatt ttgattttgc
    37561 tcattttgat cagatggctg atatcttgaa tcagtttcca ttgacctatg tcaatgctat
    37621 caacagtgtg ggcaatggtt tgtatatcga tacagaacaa gaagcagtag tcatcaaacc
    37681 aaaagaaggg ttcggcggca ttggcggaga gtatatcaag ccgactgctt tggcgaatgt
    37741 tcgcgctttt tatacacgtt tgaaaccaga aatccaaatc attggtacag gtgggattcg
    37801 gacaggacaa gatgcatttg aacatctgct atgtggcgca agcatgttac agattggtac
    37861 agagcttcat aaagaagggc cggaaatttt ttctagaatc ataaaagaat taacgcagat
    37921 tatgtcagaa aaaggatata cgtcaattga cgaatttaaa ggcaaattac gcaccatttc
    37981 atagcaaaga gacgaaggtc atgattgatc ttcgtctttt tttataaaaa acactcgtct
    38041 gttcattcct gtgcaagcgt actcattgta tgttagacga acttgtggga aaataacaga
    38101 ggcaaaaagg aggataatat gaatgatgca acagaattag caaaagagct taaagaaaaa
    38161 gaatttagtt ttcaagaact agttacggat atacagaaaa aaataaagaa gcaaaatcat
    38221 gaattgaatg cgtttgtcgc gcttgaacca ttcgagagta attcccacga gctattgatt
    38281 ccccagtcag aaaaagggcc attggctggt atcccatttc ctttaaaaat gctcggtcaa
    38341 gaaaaaaagg gatggctagc tacttcagga tctcgtttat ttgaaacaca ccgagcttct
    38401 catacatcta attatgtgcg gcaagcagaa gcgataggtc tagttccttt tggacaaacc
    38461 aatgcgccag agtttggttt caaaaatatt acagacccag tcatctatgg cccagcaaga
    38521 aacccttgga accttgaatg tacaccgggc ggatcaagtg gaggagctgc tgctgcagtt
    38581 tcatcaggaa tcgttccttt agctggtgct agtgatggcg gtggttctat tagaataccc
    38641 gcttcgtttt gcggattgat tgggctgaaa ccttcgcgag ggacgatgcc agttggtcca
    38701 tatgcttgga gaggatggca aggagctgct attgatttcg gcttgacagt ttctatgaga
    38761 gacacggagg ctttatttta tgggatgaga agtatccatt ccggtgcgcc atatcaagta
    38821 tcgccagttg agtggcaaac acatccaaga aaaaaacggt tgaagatagc tttttgtgtt
    38881 gattcaccga tccactcaca gattagtgaa gaggcagtac ttgctgtaaa aaaagctgta
    38941 gatttccttg gaaaagaagg gcatgatttg acagagattc cttacccact agatggtcgt
    39001 aaactgatca cgtcttatta tcagatgaat ggcgcagaaa cagcagcaat gatccgctcg
    39061 attgaaacag gcatccagcg agctgtcaga aaagaagaac ttgaacccat ttcttggact
    39121 ttgttccaat atggtcagaa gctctctgcc gctacttaca tcgattctct gcatgtatgg
    39181 gatgaagcag caattgtcat ggaaaaccta catcaagaat acgatctatt cctttcacca
    39241 acgactgcat ttaccgcccc taaaatcagc gaagatttgc aaagtgatgc gataagagca
    39301 cagatggaac acatcagtga ttgttctgaa gtagaaggtt tagacgttgt ttcttccatg
    39361 tttgaggaga gcttgaagat cacaccttat acacagttgg ctaatctgac tggacaacca
    39421 gcaatcagct tgcctactca tttgacaaaa gaaaacatgc ctctaggaat ccaatttatg
    39481 gctgccagag gaagagaaga tttgctattt caaatcggct atctatttga agcaaatcat
    39541 cagttccatt taccaaatcc atggaaatag cagaaacaag gagaagaaga atgaagaaaa
    39601 aaagattgtt tgctctaata actagtgcct tttttatttt aagcgcttgc ggtacagctg
    39661 ccgctaatca ggaagataac agctggagta atgtagaaaa aaagaaagaa ttagtagtag
    39721 cgacttctgg cacactgttt cccgcatctt attataatga tcaaaatgaa ttagttggtt
    39781 atgatgtaga tttggcaaaa gaggtggcta aacggttaaa tgtcaaaatc tcctttaaag
    39841 aatacaacgt ggatggacaa atcacttctg tttcaaaaag agaatcagat tttgctgcga
    39901 acgattttgg tatcacaaaa gaaagagaaa aaaaattctc cctttcagag actttcaaat
    39961 attcatttga cagtatgatc gtacgaaaaa aagatgattc aggaatccac tcacttgaag
    40021 atcttaaagg gaaaaaagca gctggcgaac ccaatactag ctatatgcgt ttagcggaaa
    40081 aacacggagc taaattagtc acatatgaca atgccacgaa tgatcagtat ttaagcgatg
    40141 tagcaaatgg tcgaacagat gttattctaa atgattatta tctgcagaaa atggccgtat
    40201 ctgctttacc agatatccct gtaaaaatac tggagaacgt ttatttcaat gaaaacgaaa
    40261 gtggtttttt attgaaagaa ggaaatgatg ctctgaaaaa gaaaatcgat ggcgtattaa
    40321 aagagatgaa agaagatggg acgacaaaga aaatatctga aaaatatttt caaactgatg
    40381 tttcagtcaa accgaaagaa acgatggctg agtcggtgag tgagtgatgt actttcccct
    40441 aattgatttt tccctcatgt ggacttcact tcctttcgta ctgcaaggct tggtatacac
    40501 attagggatt ggcttcgtgt cgtttctttt aggaaatttg gtagggttgc ttttgacagt
    40561 tttaggtctt ttggattggc tgcctctgaa tgtatttatt cgtttttatt tatcgttctt
    40621 tagaggcatt cccgcactag tcctgctgtt tctgttatat ttcgggttac cttatcaatt
    40681 aagtgcctta acggcttcag tcatctgctt tacgattacc agcagtgcat ttattggtga
    40741 aatctatcga ggatcactag caggcgtttc ttcaggtcag tgggacgctg gtttagcttc
    40801 tggtttttct ttctttgggg tattgaaata tatcgttttg ccgcaagctt ttcgaatctc
    40861 agtaccggca ctcagtaacg tagctatgga tctagtgaaa ggtacttcgt tagcagcaac
    40921 aatcacagtg ccagaaatat ttcaaaaagc aaagatcata ggcggtcgga cattcgatta
    40981 tttttctctc tatattttag ttgcactgat ttattgggtc atttgtatcg ggatcgaaaa
    41041 agtgcaacag tatttagaaa aacgttctat ccatcagtca atctgaaaaa tagaaatggt
    41101 gctagcgtgg ctggcaccat ttctattttt tgtttcttgt atattctgcc tagtatttta
    41161 aaaccatttc atgtaaaatg taaatcgaaa atatgttcgg aggtagaaaa catgaagacc
    41221 ccgctaacca tcgaaataga atcctcgtta taccaatatt gcatcgagca aggcggcttg
    41281 gttgtagaag aagtcacgat gcctgatgac caaggaattg tagatacgct tagctgcttg
    41341 acaaaatttg atggaagccg agaatggcga tgctatgagc tgaaagtgac aaaagcagat
    41401 tttcgctcta cagccaaact gtcatttgtt ggacattata attattttgt tttaccaagt
    41461 aaactatatg aagaagtaaa aactgaaatc cctacaggaa taggtgtttt agtgtatcgt
    41521 ccgtacacgg ttgaagagga gatgcctgcc tctggtacat ttctgattgc gaaaaaagca
    41581 gcaaagaaag agcttgctgt accggaagaa gctttgacaa accgcttcat ggcttcttta
    41641 ttcagggaag taagaaaagc aaagcaaatg gagcgaggcg taaaatattt tccaaaagat
    41701 caattgtaca aagaattgaa aagacgttca gaaaatcaag atccattttc taacgaacga
    41761 tattatgaac ggtttttaga ggaaatagct agtcaggcaa tcaatgagtt gcaagaagaa
    41821 gttatcgcgc ttcagcatga ttatgaatat ttgaggcaac aacagcagat ccgtcggttg
    41881 cctacagagc cattggagtg aaaaatatgt actatcctta tttaagagga aaacaatttg
    41941 acctattagc attaaaagaa gcactgtcgc gaggattgct ctccaataaa atccaaccag
    42001 tgatcgaacc agtacgtgat tccgctacgc ttaaaaatgt gatcgaatta tttcaaaaaa
    42061 agcaccatcc aatcgctgtg atccaaaatc cccaagtagg acaattcaaa ttattcgatc
    42121 agcatgtgca cagttggaca gtcaaagaaa atagctctgt cgtccctgca cagatcttaa
    42181 cgccagaaaa tctcagtgaa gtactggatt cacctccagc gtttcttgtt ttcgatggac
    42241 agcactaccc aaaagataca gaggtgtgga aacagttggc aggagttgat tcgaaatttt
    42301 tgataccaga tactagtcgc tttcgcatct ggctgcccga aaacaaaatt gtgatccgtg
    42361 actcttttca aacgagaaag catgttgaaa gttatgcaga taaaacagat gactttttta
    42421 gtgatgacta tctttttttc cacgcagacg gatatattgg attttctgat ttcacgatcg
    42481 aaggtagccg ttatttcgat aaagggttcc ctagccgagc gcttgctatc catctaacgt
    42541 atattgatgc ttacggcaat atacgcgtga aacattttgt ctctgacagt aacgactctg
    42601 ctaaagacca agcattgaag tttcttgaag caggagataa aatgaaaaaa tggatcatgc
    42661 gtcaccatca tcaactcttg attacttcag gaatgattga attattggct ctttaccagc
    42721 aacaaaaatt ccctggactg ggagtgttga aaaagtggtc acttatgcat catttggaac
    42781 ttattagtca cctgctagat catccaaaag attggcttaa atattattca aaagttcagg
    42841 aactatatga agtgatccaa tgattagata atgtaaatga aaaaggaata gaaaagagga
    42901 aactggatgt atattcggca aacaaggaaa gaagaagtag aagaagtggt agaaatcatt
    42961 aatgccgcag cggatttcat tgcttcgcaa ggctctcctc aatggcaaaa cgggcagaaa
    43021 ccaacaaaag aaacgatttt gaacgatatt gaaagaaaag aaagctatgt attaatagtg
    43081 gaagatcaaa ttgctggaac agctgcatta gtctcaggcg tggatcccgt ttacacaaaa
    43141 atagatggta gttggaaaga atcaactggt ccttatttat cgattcatcg tgtcgcttta
    43201 gcagaaagta ctcgaggaaa aaaactagca aaaccgttct tagaacattt gttgacgatt
    43261 gcagccagca aaaatatcca agatatccgt atcgatactt atccagctaa tgaaccgatg
    43321 gaaaagacca tctattctgc tggattcact tatcaaggga tgattgaatt tccttttgaa
    43381 cacggcgaaa gaaaagcata tcaaataatc atcaattgac tataaaatca tctgagcaaa
    43441 gataaagaca ttaacactac tgaaaaaaac aatcatatga tagccaagag gttgtaacag
    43501 aagtggccag cttcaaaaaa caagaaagaa tttccgaaaa tgcttctcaa atttttgtgg
    43561 aattttagcg tattttacga aatagctgct ttctgaaact cccttcatcg agaggtcgtg
    43621 acatagcttt tgtcacgatc tctcggctta tcctacgagt attattcgat acatttatat
    43681 caataatcct ttttttattt tatctgcagc ttcttggaac gctccatcgt ttgacacgac
    43741 taaaagtgag gggatagctt tcaaacgttt caactgtgct aaatcttcta cataaaccgc
    43801 cagtctggct gaaatctgtt cagatgaatc tccacgtttt ttcaggcgcg attgcaatgt
    43861 ttctttagag atcgtaataa agataggacg gactaccgtc ccgaaatact catataaatg
    43921 ccaaaaacca tttgctgtca aaacggcgta acaatttcct actgtcagtt tctttttaat
    43981 ttctgcttta ctggaaccat atttatgttg agcatattgg tcccactctg caaactcatt
    44041 tcccttgatc atctgattga atgtattgtc agaaacaaaa taataatcct cttgatcttt
    44101 ttccccaggt cttggtgatc gattcgtgta agtaatgatc ttttgttccg gctgaaaaat
    44161 agcttctgcg agtgaagttt ttccagaacc agaaggaccg ataaatacat agcaataatt
    44221 cattgtcatt tctcctttga tgattgaaca agtgaaaaag ctttggaaaa tatttccaaa
    44281 gcttttaaaa gtaactttgc ttttttgtat tttataccag caagccagtc acaatagcac
    44341 ttaaaaagct gactaaagtc gctgttagca aaattttcaa tccgtgtttt gcgacaagat
    44401 ttcctttttc ttcatttaat cccttcatcg caccagaaat gatcccgata gaagagaagt
    44461 tggcaaatga aacgaggaag acagatataa ttgctgttgt acgctcagta aaatggaaat
    44521 taccatttgc taattctgtc atagcaacaa attcatttgt aaccagttta gtcgccatga
    44581 tactaccggc atccactgct tctttccatg ggattccgct gatgaaggca agaggggcaa
    44641 agatgtaacc taggatctct tggaaagtga tgccgaatat cccgtggaag atagcattga
    44701 tcatggcaat caaagcaaca aaaccgataa gcatagcagc aaccgtgatc gccacatgga
    44761 aaccatcaag aatgtattct cccaacattt ggaaaaatgt ctgttctttg ttttcttcaa
    44821 tgatcagctc atcttctttt tcttctaata catagggatt tacaattgaa gccagaatat
    44881 aaccgccaaa taagttcaaa acaagggcag tgatcacata tttagaatcg atcagtgcca
    44941 tataagatcc gacgatcgac atagaaactg tagacatagc cgatacactc atggtgaaca
    45001 aacgtttctc agataggaag ggtaattcct tttttatgga aataaaaact tccgattgtc
    45061 ctaaaatagc agaagctact ccattataag attccaattt gcccatcccg ttgattttac
    45121 ttaatcctag accaacagct ttcataaata atggaagaat cttgatatag cgtagaatac
    45181 cgataatagc agaaataaat acaattggca tcaatacgga aagaaagaaa ggtgcacttc
    45241 ctttatttgc tacaccggca aaaacaaagt caacaccttg cccagcaaaa gcaagaagat
    45301 gatcaaacat ttttgccaat acagaaacaa ccgctgtacc aaaagttgta cgtaataata
    45361 cgaaaccaag aacaagctgt aaaacgaaca tcacaattag tggtttcaat ttgattgctt
    45421 ttttgttact gctgatcaaa aaagcaagcc ctaatgtgac aaataaacca ataataccta
    45481 ataaataact caaataatca ctcctattcc aaattccgtt ttattttacc tgttttgcaa
    45541 ataaaatact aatctttagt gaaattttca ttactttttt agagtgaaac gcattcattt
    45601 tccattagaa aataaagcga acaaaataaa aggtttaaaa agtgtaacgt cttagtaaag
    45661 tctataaaaa aacgaaacta tcgattaagc taatagtttc gttttcaaca tttataagta
    45721 aactttttct attggtaata tagattttcg gaagggtaga caggtgcgca ggtaatatcg
    45781 attcctagtt ttttcaatgt ttgttcatca tttttattga ccatcacagt agagtgggct
    45841 tgtgctccct caagttcagc tagtttatca taagcaactt gagcgattgg attcgttact
    45901 gcactgattg ataaagcgat caagacttca ttgatgctta gtgcatccaa agaggaatga
    45961 agtgtctttg atttcaatcc ttgtattgtt tcaagaacta atggtgacaa taaaggaatg
    46021 tcatctgcga tatgggctaa atgttttact gcatttaaga taactgccgc tgacgcatcc
    46081 atcaatgaac ttgtacgccc ggtaatgatg acttcatctg gtaattcgat tgcaatcaca
    46141 gcagaaggat cttttgtagg actttgcgct tgaatcttat ctgcatagtc atgggcaggg
    46201 actaccacgt ttcggtcttc aggacgtaaa ccgacttctt ccataatcag tttgattcga
    46261 ttaactgctt cttcgtcgat caatcctttt ttgaagtcgc attctgtttg gaaataacga
    46321 cggatgattt cttgtttaga ggcttcttgg acaacggtat catccacgat cccaaatcct
    46381 acacgattca cacccatgtc agtaggagat tgataaacag attctttacc agtaattttt
    46441 tcaataatac gtttgattac aggaaatgtc tcaacatccc gattatagtt cacagctact
    46501 ttattgtagg catcaaagtg gaaggaatcg atcatattga catcttttag atcgactgtg
    46561 gctgcttcat aagcaatgtt cagcggatgt ttcaacggaa cattccatac tgggaaagtt
    46621 tcgaacttgg agtaaccagc tgcatgtcct ttttggcttt catgatacag ttggttcaag
    46681 caagtagcga gctttccact tcctgggcca ggagctgtca ctacaacgat tggtttagtt
    46741 gtttcaatat actcattttt tccaaaacca tcttctgaga cgattttttc tacgttgact
    46801 ggatagtctt cgatttctga gtgtttatag accttgatgt ttcgtctttc tagtttattg
    46861 ataaagactt tcgtggcagg ctgtccacta tagcgagtga taacgacact gttgattaat
    46921 aagccataac cacgtaattc atcgattaac cgcaagatat ccatatcata agtaatccca
    46981 taatcaccac ggatcttatt acgttcaata tctccagcat agacgcaaat cagtatttca
    47041 gcttgatctt tcaatttttg cagcaatttg attttcgcat cttcttcaaa tcctggaagt
    47101 acacgtttcg catgcttgtc gtcaactaac ttcccaccaa attctaaata taatttgtca
    47161 tagtggttca ctcgttccaa aatatacgct gactgctctt caatatattt ttctgaatca
    47221 aaaccaattt ttttcactag gttccctcct taagtactaa agcatttttt catttttttg
    47281 aggattcgtc aactggaaga gcgaaattcc tcaaaaaaac aaagaaaatg gtttaaacca
    47341 acttgtctag atagttattc attggtatga tagtttttga taaggaaggt gctacatgaa
    47401 aaaaagacag aaaaagaaaa acgcatataa acactatatc cgttcgattt ttaccggtta
    47461 tgaaagaatg cttgaagatc ccgaattgga acaattgacg tttacttatc taaacgaaga
    47521 aacgcagcta actcgtgatg agcatcagcg cattcatttc acaacaagag atctgcctag
    47581 taaataaaaa ataagataaa ggtcatcgga ttcgattctg tcctatcaga tggaaagact
    47641 agttgatcga aaaaaaggct gtaccaaatt ttggtgcagc ctgttttatg gagcattgcc
    47701 cgctcttttt tataacaatt ttttacagaa aatggtacta ttgaagtatt aataagtgaa
    47761 aaacagatga gtaagaggag gcaagtagta tgaaagagcc agatacaaat gcaaattatg
    47821 agccagacga actagaggag acgaacgcgg atcaggatac aggacaagaa tcgtcttctt
    47881 tttacgatat caaagaaaaa gcaagcatga atctttccca attattagaa gacttacagg
    47941 cttttgaaaa agctgtccgc gaagaagata tccctgagat ctaccgttta tacaatggcc
    48001 gattgaatga agaattaaaa agaagctcaa atgaaaatca cgagatcgat caattgctga
    48061 tgcgtaagat ccatgatcgt tttttacagg aatttccttt tatggaacaa gtagaaaaca
    48121 tctcgccaac gatgagctat tacaaaatcg gcacttatta ccatgatcgg cccacggtcg
    48181 gtattgatgc aagtctgcca gaaatttttg ttttgcctaa gatcgatgaa gagtgggaga
    48241 aatattccca accggagtca acggactatg acaaaaaaat caatgagcta gacgcaaagg
    48301 tgatcactgc tcagacagaa attgaacgtt tagatgaaca aataaaagaa atcaatcgac
    48361 aaatgactga tttgaacgac aataaaggat tcttgaatcg taaaaaaatc gaggaagaaa
    48421 ttcaagaatt agagaagaag aagcaagtac tatctaatga aaaattaggc tggcttcctt
    48481 atattgagac gcctgaaaca atccagcagc aaaaagaagc attgaagcaa gaagcaagag
    48541 cagaccagct tcgtgcagcc atcgtagaaa aagaacaaag gcaaatcaga cgctatttcg
    48601 gtagcaaaga gggattcggt caagcaatcc atgaattctt gatgaattat cttggcaatg
    48661 agaatcctaa taatcaatct aatgaaggag gttccgagta tgaatgaaca cgatgaagca
    48721 gaatggacaa aaaatgaaga acaaacagaa gatcatgaag aaacgaacac ttcattggaa
    48781 gaacaaacaa gtaatcctct ggatgacaca gaagaaatcc ctatgctaga ggaaacaaaa
    48841 atacatgaaa aagaaaaagt aactccgacg aatacgaata ctgcagaaaa cacccatgct
    48901 tacgaaataa ctgaaaatcc cgggtattta gaaggaaaag agcaagatca tcaagatatc
    48961 gtcagtttga ttgaaacagc ggaagcagaa ttagcggatt tacgtatgag aaaagatttg
    49021 atcgaatcag attttccaca attattagaa tcatatgatc gttttatatt gaatgaagaa
    49081 ccaatctcca cgatcggaaa gacaagcctg atcgaatact tcacgtatga attactgaaa
    49141 ccgttgaatg atattggtct ggaacggtca aattcagctt atgacacatg gaaaacaaga
    49201 catttttcga tcaattatac gttgaatgga caaaacgaat tagtcttttc ctttgtatta
    49261 ccgaccatcg atcaacgata ccaagtggaa tcgatgagtc tcctaaacgt atcaccagag
    49321 acaatggaaa tcaatgtcca agatgaccgg gtgctttctt tgatccgaca ctggtcagtg
    49381 gatcgagtat tctctgcagg gcagatcacg atattcaatc acaaattgaa tcaattatta
    49441 gcccatgcaa gaacacttgg ttttaccgtt aatcagacat tactagacaa tacgaagcca
    49501 cttcgtttga atcttcagag cgaatttgaa ttgacgaatg aagtattgga tgatattttt
    49561 atcgtggcaa tgaaaaatcc atcctacgat atggaaaaga tcaacgaaac tacttatcaa
    49621 gtacgattag ataaaggaca gtctttaacg ataacaacaa acgaaaaaga tcaaacagtc
    49681 ttgtcgatct catctggaga ctacccacga tctgtcattg atttctttat caattacgaa
    49741 ttcttggtcc cattaatggt gagaaagtcc tgattggata agttggatta ccagatgtca
    49801 ttcagataat tctgttccaa aggacttgcg gcagttatcc agaataattg tcacaagtct
    49861 tttctgtatg tactaaatgg acgtgaataa cttgctgatg aaggagggat ttcattagga
    49921 atgtccacaa ataaaatagc acagcttagc ttattgtctg ctgcttgtgt agcaggtagg
    49981 atcgcttttc aatttattcc gaatttccag ccaatgactg ctattttttt atttatagta
    50041 ctttacttaa gcttaaaaga tgcgttaatt gtcatgagtc tttctattgc gatcagcagt
    50101 ttttattttg gtgttggacc atgggtcatc ggccaatgga tcagctatac tgttgttctg
    50161 agtttgtttt caatcgtctg cttgagaaaa acagtgcaaa ggaatctatg gttaaaggga
    50221 gtctgttttt ttctggcggg catggtttac ggaataggta tgaccgtttt tgatacgctg
    50281 ttatatcgtt tgcctcaacc ttggatctat taccttcaag gggtgtcgtt tgatttgatg
    50341 cacagtatag gcaatgttgt gttctttctt gtttttctgc cagtcgtcaa acgtttttac
    50401 aatcattcag gaggaaaaaa tgaaaaaaat aatttatagt ttgcttgcaa tgagtgtcag
    50461 tatactcatt ttgacaagtt gcagtacaac ctatacacaa tcggcttcca accaaaagga
    50521 ggaagtaaaa acatcgcaaa aagcaacaat cacgttacaa gaaaatggtc aaaacttttc
    50581 ggaaaaagaa gtggtcttca aaaaaggcga tgatttactg agtattttga aacgaaattt
    50641 tgaagtcaaa gaagataacg gcttcatcac ttcgcttgaa gggcactcac aggatgagaa
    50701 aaatcaactc tattggatgt ttactgtgga cgggaaaagt gctactactg gtgccaaaga
    50761 gatcaaattg aatgatggac aatcggtcat tttcaatttg tctaaactat aagtttcata
    50821 aaaaactgtt aaagggcgaa tattgccata attcagtgag ttcagttgat gaaagaagga
    50881 aaataacatg gaaaatttta aaaaaagcgg aatgttttcc gtaattgctg ctctaggggc
    50941 taatattcta gttgctatca gcaaatttgt cggctatgca atcagtggca gtgccgccat
    51001 gctaaacgaa agcatccaca gtatcgttga ttgcagcaat cagattttgc tgttattcgg
    51061 tgataaacaa gccaataaag gacagagcga gttgcatcaa tttggtgaag gacgagcaaa
    51121 atactttttc agtacaatcg tcgcaatgat gcttttcttc ggcggtgggg cacttggggt
    51181 gatggaagct tttgaaaagt tgacgcatcc atcccatgaa gtgggtaatc cgatcattgt
    51241 tatagcaata ctgttatttg gtttggtaat cgaaggaagt tcgttacgcg tggcaatgaa
    51301 agaaatcaaa gaattgaata ctgaaaaact ttccacgatg cgctttttgc gcgaaagtcg
    51361 acacagtgaa atactgatta tttttacgga agacttgtgt gctgtaatcg gtttggtctt
    51421 ggctttagca ggaacaatat tgacttatgc aaccgggatt gctgcatttg atgcgatcag
    51481 cggattgctg atcggtttct tgctgatggg agccgccatt ttcctagcaa aagaatttta
    51541 tagtctgatc atcggtgaaa gcgtgacagc aagcgattta tcaaaaatca agttagcttt
    51601 tgaacgtcct gagatcagtc ggctgatcga tataaaaaca gttcatttaa gtccaacgga
    51661 gattttagtt gcagcaaaag cagatatcgt tggtaacaaa gagaatgaag cttactcggt
    51721 catcaatgat attgaaaaaa atatgcgtca gtctcttcct gacaagaaga tgtatatata
    51781 cattgaaaca gataaatatg acccaaatta ttctcgtaaa taaaacgttt attcatgaat
    51841 tgaagcaaaa atgagaaaaa tataatcttg tttttaaata aaacggctac aatttagcga
    51901 atttatgtta caatgagtcc gctgtttatt tgttgtaatt aaaggagata ttttaatgaa
    51961 caaaaagaaa agtgcgccat ggcttcaagc tttgcatgca gtgatgccac tttgtataag
    52021 ctatatacca gtaggattag cttgcggagt attgttgcaa aaagttggat ttgatcctct
    52081 cttatcagga ctgttatcga ttctggtctt ttccggtggc gcgcagtttt tagttgcatc
    52141 tatgcttacg acgcaagcat catttgcaac gacattattg atggttttct ttttagaatt
    52201 acgctataca ttattaggag caagtcttgc cggttttatg aaaaaagaaa agcggacgtt
    52261 tttggctgtc ttttctcaat cactgaatga tgaaaactat gcggtgaact acttaaagtt
    52321 ttcaactgac ccttcctgga ataaaagaaa ggctttatat gtcaattggt tctcaatgac
    52381 tgcatgggcc ggtagcaata tgatcggtaa ttttttcggc tcggtgattc gagtagatgc
    52441 tgaccttgtc cattttgctt tgacggccat gttcattttc atgtttatca tgcagatgaa
    52501 aagcgcacta ctgatcttta caggtttatt ctcaggcgta ttaggggtcg tattcatggt
    52561 attattccaa aatactttcg gattgattgc agcaacagtc attgcttcat ttgccggatt
    52621 tatgctggag aaggcattca aaaaagaaaa agctaagaaa ctaagagacc gcggtaaaga
    52681 tgtcaatgag ccgctcttca acttcccgga acaggaggcc aatcaccatg actaaactat
    52741 atttgctact tttagttgct tgtctgttca tcgtggcata tatcccgcgc ttgttcccga
    52801 tgctttattt tactcatcga aaagtgccat cctggttcag tgactggatg aaatatgtac
    52861 cggtggcatt atttgcggcg ttagctttta aagatgtttt cattactcat gaacatctgg
    52921 atattgcttg gaatatcaaa atagctgcaa tgattctagt agcaggcgtg gcttacaaaa
    52981 cacgttcgat ggcgttgtca gttttgacag gacttgcgtc tgtctttttg ctgtctatgc
    53041 tttaaactgt tttattttat taaaaacaga gattgcgaca taagttgttc aaactgatct
    53101 atccgaactg catttgcaac agtcaattct ggatgcaagt ttattcgtag ggataaaaaa
    53161 gattagtgtt ggcttatgat gcaagctctt ttttatttta ggaattagag agggatttgt
    53221 ccttctatga ttttttgcta taatagggaa gatgtttatt ttatgaaaga atagttgagg
    53281 gaatttaaat gaataaaaaa ttgatgcaag gaacattttg gctaaccttt gctaatcttc
    53341 tatgtaaagt tttaggggta gtctatttga ttccctggct aagtatgatg gggaacaatc
    53401 aagatggtat gcttgctacg acattgtata atgttggcta tcttccttat ggattgttcc
    53461 tgatgcttgg tacagtcgga tttccaaatg cgattgcaaa aaaagtagct gtagccacaa
    53521 aagaaggaga taacgctgct tgtcggctga tttttagaag tacgatcaat atcatgtttg
    53581 tcatcggtat cgcctctgct gcactgatgt atttatttgc tcctttacta gccggaatca
    53641 gtccgattgc taacgttaat aatgggatac tagctatcag aagtttatgc ccatctttga
    53701 ttgctatccc aattcttagt gcaatgagag gatatttcca aggaaaaaat gatctgcggc
    53761 cttatggaac ttctttgatt atcgaacagg ctgtgcgagt gatcgttatc ttggctggta
    53821 cttattactt gcgcgtattg actgacggaa ccatattaga agctgtattg atcagtacag
    53881 tcgcttcttt cttcggtggg ttagctgcga ttatccatat gtatatcgtc ggacgcagaa
    53941 aagacttctt cgaacttaaa gattttttga tttccagccg ttatttcgaa agagaaaacc
    54001 gatctgcctc tgtctctatc attcgggaaa cactaccgtt tatttttgtc ggctcagtca
    54061 ttactatcgt tcaaatgatc gaccaagtaa cgatgaaacc actgcttcac tttttccgtc
    54121 cagagatcgc agatcagcaa ctggaattct tattcagccg tgcttctgtc aatccaaata
    54181 aattgacatt gatcctgatc tccatggtag gaacagtagc tataagcagc ttgccaatat
    54241 taagcacgat gaagaaaaaa gatcggatac aaatcgaaaa aacagtagga gatagttttt
    54301 cgattgcgtt attgattctt cttccttcat tgactggaat gtctttatta gctggaccac
    54361 tatacacatt gttttttggc tatgatcctg aaagtgtcgg atatttccaa atggctttgc
    54421 tagcttcttt gtttttctct ttattcacga tcctatctac gatgcttcaa tctttgaatc
    54481 accatcgttt tgcgatccga ttaactgtag aagcaattat cctgaaagtc atcttccaag
    54541 taatcggatt aggattagtt ggcggatacg ggatgagctt atcaaatggc ttagcattcg
    54601 gtattgtctt tgttcgcggc tatcacttta tgtgcaaaga ataccgaata gcacctttag
    54661 caaagatcag caattttttc ttaaaaactt tccgctccac cctgattatg cttgcagtat
    54721 gttttgccgt tttctttttg ctgaatcagc gattgtcaat ggaatcaaaa agctatgcaa
    54781 tgatttattg tgtggctgta ggaagtattg gcggcttagt atttttattt tctcaattcg
    54841 gaaaaaatgg gtttcgaatg ttgaaaaatg ttaaacatca tagcagaagc aaagaatagg
    54901 ttaactaatc gttttttgct gtcacttgaa aaacggcaac tcgtatgatt acgcttgtcg
    54961 tttttctatt ttccaatttt agtttcatat ctaatcttac ttatgatata gttataaggt
    55021 ctggaaaaat tctttgaaac gaggaaatta aacacatgaa acttgcagta gttacagata
    55081 gttcggcata tctgccggaa cgtatcaaga accatcccga cttattcgtt attccaatcc
    55141 ctttgattat ggatgggaaa atttataacg aaggaatcga tatcgaagcg gatgaatatt
    55201 atggactttt aaaaaacagt aaagattttc ctactacttc acaacctgtc gtaggggaag
    55261 ttttgggttt atatgaggaa ttgaaagaga aagggtatga cacgatttta agtatccatc
    55321 tttcttcagg gatttctggc tttgtcaaca cattatttgc gatgaaagat gacatcagcg
    55381 gagtaacggt cattccttat gactctaaga tcactagtat gccgatggga cacatggtag
    55441 aagctgcctt agacttaaat gatgaaggtc agtcccttga tgaaatactt gcacatatcg
    55501 atcgaatccg agataatacc tatgcttatc tgattgtaga tgatttgaac aatttagtac
    55561 gaggaggacg tttgacaaat ggtgcagcgc tgattggcag cttattaaaa atcaaaccga
    55621 tcctgacatt ttctgaaggg aaaattgttt tattcgaaaa aatccgttca agtaaaaaag
    55681 catttgctcg agctgaagaa attattggta gaagaaataa cgaaatacaa ccgccagtca
    55741 aactttacgt gatacatgca aataatttag aagtagcgat tgcggagaaa gagaaattgc
    55801 agaaaaaata tccaaatgca gaaatcgaaa ttggtcattt cggaccagtc attggtacac
    55861 atttaggtga aaaagccatc ggcattgcca tttcagcgca ataattcctt ttattcaaaa
    55921 aacataccaa aaatat
//
LOCUS       DACDYJ010000009        55795 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDYJ010000009 DACDYJ010000000
VERSION     DACDYJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.009"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            336..1439
                     /locus_tag="IWM51_000539"
     CDS             336..1439
                     /locus_tag="IWM51_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9969979.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            1723..2403
                     /locus_tag="IWM51_000540"
     CDS             1723..2403
                     /locus_tag="IWM51_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9969980.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            2493..3527
                     /locus_tag="IWM51_000541"
     CDS             2493..3527
                     /locus_tag="IWM51_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9969981.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            3541..3876
                     /locus_tag="IWM51_000542"
     CDS             3541..3876
                     /locus_tag="IWM51_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969982.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3860..6340
                     /locus_tag="IWM51_000543"
     CDS             3860..6340
                     /locus_tag="IWM51_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9969983.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            6396..7955
                     /locus_tag="IWM51_000544"
     CDS             6396..7955
                     /locus_tag="IWM51_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9969984.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7952..9316
                     /locus_tag="IWM51_000545"
     CDS             7952..9316
                     /locus_tag="IWM51_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9969985.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            9487..10557
                     /locus_tag="IWM51_000546"
     CDS             9487..10557
                     /locus_tag="IWM51_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9969986.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10572..11240
                     /locus_tag="IWM51_000547"
     CDS             10572..11240
                     /locus_tag="IWM51_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9969987.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            11260..11838
                     /locus_tag="IWM51_000548"
     CDS             11260..11838
                     /locus_tag="IWM51_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9969988.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            12241..13152
                     /locus_tag="IWM51_000549"
     CDS             12241..13152
                     /locus_tag="IWM51_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9969989.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(13408..14325)
                     /locus_tag="IWM51_000550"
     CDS             complement(13408..14325)
                     /locus_tag="IWM51_000550"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP9969990.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14527..14964
                     /gene="zapA"
                     /locus_tag="IWM51_000551"
     CDS             14527..14964
                     /gene="zapA"
                     /locus_tag="IWM51_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP9969991.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15022..15552
                     /locus_tag="IWM51_000552"
     CDS             15022..15552
                     /locus_tag="IWM51_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010781150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP9969992.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELW"
     gene            15684..18044
                     /locus_tag="IWM51_000553"
     CDS             15684..18044
                     /locus_tag="IWM51_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9969993.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            18131..18445
                     /gene="trxA"
                     /locus_tag="IWM51_000554"
     CDS             18131..18445
                     /gene="trxA"
                     /locus_tag="IWM51_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP9969994.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18627..20405
                     /gene="uvrC"
                     /locus_tag="IWM51_000555"
     CDS             18627..20405
                     /gene="uvrC"
                     /locus_tag="IWM51_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP9969995.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20550..21449
                     /locus_tag="IWM51_000556"
     CDS             20550..21449
                     /locus_tag="IWM51_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9969996.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21442..22704
                     /locus_tag="IWM51_000557"
     CDS             21442..22704
                     /locus_tag="IWM51_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9969997.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22867..24765
                     /locus_tag="IWM51_000558"
     CDS             22867..24765
                     /locus_tag="IWM51_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP9969998.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(25064..25783)
                     /locus_tag="IWM51_000559"
     CDS             complement(25064..25783)
                     /locus_tag="IWM51_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP9969999.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            26120..26452
                     /locus_tag="IWM51_000560"
     CDS             26120..26452
                     /locus_tag="IWM51_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970000.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26439..28442
                     /locus_tag="IWM51_000561"
     CDS             26439..28442
                     /locus_tag="IWM51_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP9970001.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28461..28931
                     /locus_tag="IWM51_000562"
     CDS             28461..28931
                     /locus_tag="IWM51_000562"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP9970002.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28952..29536
                     /locus_tag="IWM51_000563"
     CDS             28952..29536
                     /locus_tag="IWM51_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP9970003.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29551..30537
                     /locus_tag="IWM51_000564"
     CDS             29551..30537
                     /locus_tag="IWM51_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP9970004.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            30527..30838
                     /locus_tag="IWM51_000565"
     CDS             30527..30838
                     /locus_tag="IWM51_000565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP9970005.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30860..32641
                     /locus_tag="IWM51_000566"
     CDS             30860..32641
                     /locus_tag="IWM51_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP9970006.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32634..34010
                     /locus_tag="IWM51_000567"
     CDS             32634..34010
                     /locus_tag="IWM51_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP9970007.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34017..34652
                     /locus_tag="IWM51_000568"
     CDS             34017..34652
                     /locus_tag="IWM51_000568"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP9970008.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34682..36037
                     /locus_tag="IWM51_000569"
     CDS             34682..36037
                     /locus_tag="IWM51_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP9970009.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(36229..37623)
                     /locus_tag="IWM51_000570"
     CDS             complement(36229..37623)
                     /locus_tag="IWM51_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9970010.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37715..38686
                     /locus_tag="IWM51_000571"
     CDS             37715..38686
                     /locus_tag="IWM51_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP9970011.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38683..39465
                     /locus_tag="IWM51_000572"
     CDS             38683..39465
                     /locus_tag="IWM51_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP9970012.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(39634..40005)
                     /locus_tag="IWM51_000573"
     CDS             complement(39634..40005)
                     /locus_tag="IWM51_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9970013.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            40139..40582
                     /locus_tag="IWM51_000574"
     CDS             40139..40582
                     /locus_tag="IWM51_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9970014.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(40625..41434)
                     /gene="yidA"
                     /locus_tag="IWM51_000575"
     CDS             complement(40625..41434)
                     /gene="yidA"
                     /locus_tag="IWM51_000575"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP9970015.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(41453..42820)
                     /locus_tag="IWM51_000576"
     CDS             complement(41453..42820)
                     /locus_tag="IWM51_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9970016.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(42804..43634)
                     /locus_tag="IWM51_000577"
     CDS             complement(42804..43634)
                     /locus_tag="IWM51_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP9970017.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            43797..44234
                     /locus_tag="IWM51_000578"
     CDS             43797..44234
                     /locus_tag="IWM51_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP9970018.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            44310..44930
                     /locus_tag="IWM51_000579"
     CDS             44310..44930
                     /locus_tag="IWM51_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP9970019.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            45119..45625
                     /locus_tag="IWM51_000580"
     CDS             45119..45625
                     /locus_tag="IWM51_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP9970020.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            45642..46541
                     /locus_tag="IWM51_000581"
     CDS             45642..46541
                     /locus_tag="IWM51_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP9970021.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            46628..47512
                     /locus_tag="IWM51_000582"
     CDS             46628..47512
                     /locus_tag="IWM51_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9970022.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(47704..47775)
                     /locus_tag="IWM51_000583"
     tRNA            complement(47704..47775)
                     /locus_tag="IWM51_000583"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47740..47742),aa:Arg,seq:ccg)
     gene            47835..49169
                     /gene="rpoN"
                     /locus_tag="IWM51_000584"
     CDS             47835..49169
                     /gene="rpoN"
                     /locus_tag="IWM51_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP9970023.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            49372..50409
                     /locus_tag="IWM51_000585"
     CDS             49372..50409
                     /locus_tag="IWM51_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP9970024.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            50450..51451
                     /gene="gap"
                     /locus_tag="IWM51_000586"
     CDS             50450..51451
                     /gene="gap"
                     /locus_tag="IWM51_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9970025.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            51561..52751
                     /locus_tag="IWM51_000587"
     CDS             51561..52751
                     /locus_tag="IWM51_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP9970026.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            52855..53610
                     /locus_tag="IWM51_000588"
     CDS             52855..53610
                     /locus_tag="IWM51_000588"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP9970027.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            53726..55024
                     /gene="eno"
                     /locus_tag="IWM51_000589"
     CDS             53726..55024
                     /gene="eno"
                     /locus_tag="IWM51_000589"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP9970028.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            55423..>55795
                     /locus_tag="IWM51_000590"
     CDS             55423..>55795
                     /locus_tag="IWM51_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HAP9970029.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGK"
BASE COUNT        18520 a         9694 c        11830 g        15751 t
ORIGIN      
        1 atcgagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
       61 gttttccaca tttttgcata aatttgactt aataccgaga aaatggcttt tgaacaccgt
      121 ttattcaaaa aaagggactt tgacactact tttgtcaaag tcccatcggt tttttatcta
      181 tttttattac aatcatttct tccagagtag tgacatctgg aatggctgaa atgtaaacag
      241 attatttgcc tgtatcaatg tcgaagagta atataaaaag tgtaaacgct atcatattgt
      301 tcatcattca aattttatag agagaaggca ttgagatgga aaaaacatat caagcaggta
      361 caaatgaagg aatagtagat tttatcaata tggaagattt agaaatcgcg gcttcacaag
      421 tgattccagc tggtggttat ggttacatca gcagtggtgc tggcgactta tttacttatc
      481 aagaaaatga acgggcattc aatcatcgtt tgattattcc tcatgtatta agagatgtag
      541 aattacccga tacaactaca cattttgacg aagaaatgct gactgcacca atcatcatgg
      601 caccagttgc tgctcatgga ttagctcatg tgaaggcgga gaaagcttct gcaaaaggtg
      661 tagcagattt tgggacgatc tatacagcaa gttcttacgc ttcttgtact ttagaagaga
      721 taagagaagc aggcggggag aaagcaccgc aatggtttca attttatatg agcaaagata
      781 atggaatcaa tttggatatt cttgaagtgg ccaagcgtaa tggagcaaaa gcaatcgtat
      841 tgacagcgga tgcaacagtt ggcggaaacc gtgaaacaga ccgcagaaac gggtttacct
      901 tcccgctgcc aatgccgatc gttcaggctt atcaatcagg agtaggccaa acgatggatg
      961 ctgtatataa atcttccaaa caaaaactta gtccaaaaga tgtagaattt atcgctgctc
     1021 attctgattt gccagtctac gtgaaaggtg ttcaatcaga agaagatgtc tatcgatcat
     1081 tagaatctgg tgctggtgga atttgggtat ctaaccatgg tggccgtcaa ttagatgggg
     1141 ggcctgctgc gtttgattcg ttgcaatatg tggcagaagc agtagataaa cgtgtaccaa
     1201 tcgtgtttga cagcggtgta cgacgtggac aacatgtttt caaagcaatc gcatcaggtg
     1261 ccgatcttgt tgctattggc agacccgtta tttatggatt gtctttagga ggaagtacag
     1321 gagtccatca agtgtttgat ttctttaaaa cagaattgga aatggtcatg caattagctg
     1381 gaacacaaac agtagaagat atcaaaaaaa taaaattgcg tgagaatcgt tttatttaag
     1441 aaataaatga cgaaaaaaat aggagtgcgg cagatgatgc cgtactccta tttttatagt
     1501 tcaaggatgt cactaataga ataatttgaa acgaaaaaat aaaattgaaa tattttttca
     1561 tacttaataa aaaaggaaga atattcatgt aaacgatttt attgtttttt tctcttttat
     1621 acaaaaaggg tcataatttc aagtaagcgg atgaattcat gaggattttt tgcatactgt
     1681 ctctactata cttaggtcac taagaaaaag gagtgattta ttatgaaaaa gaacatgatt
     1741 gggacattat tactttcaac acttcttcta ggcggcttgg caacaccagc ttttgctgag
     1801 ggtcaagcga cttcaaaagg agatattacc tttactgaac caacaaatac cgttgagcct
     1861 cttaacccta ctgatccttc aaaaccagta gagccagcag atccggaaaa tccagcaact
     1921 ggccagacag gttctttaac attggatgta gttcctgaat tgccatttgg tacacacgag
     1981 attgaaagcg gaacaaaaac gtatcaagta gacgcttcaa aaaacgatac accttatctc
     2041 caagtatcag atcgtcgagg tgtaggtgca gatggccaag cacaagggtg gaacgtgaca
     2101 gtttcggttt cagattttgt aaacggttca caagttttgc aaggcgctga attagatttt
     2161 ggaacatcca ctgttaaaag tacgtcagat aacgaatcca ctggaccaac tagtcaaact
     2221 gtaactgggt tgagtaaagc aagtgcagcg acaccaatat ttacagcagc aaaagatcaa
     2281 ggtctgggaa catggctttc tgtttatgat ccagcaaata tcacactgaa agtgccgaaa
     2341 gcagcagctg gaacattcac ggctgatttg acttggaacc tcgtggctgg cccagtagca
     2401 tagagtatga aataaattca acaaactgcg gtgaaaatac tataggtgaa ggatttttta
     2461 ccgcagttgt tgttgaaagg atgtggtgga caatgaatca aagtaaacga ctgtttgttt
     2521 cgtttttttc tattttaagt ctatttttca tttttccgtc aatcagccaa gcaaaggatt
     2581 cagcaggaga ctttggaatc aaaccggtat ttccagagaa ccaaatagat aaagcaatcg
     2641 gttattttga tttattagtg gcaccggaac aaaatcagac actagaagta attatcagca
     2701 attcttcaga tgaagaacga acatttgagg tctctgtcaa tccggcggta acgagtgatg
     2761 gtggaacgat cgattatagt caaaaaaacc caacgttaga tgagacattg ccgtttgatg
     2821 tccgtgacgt tttgctcatt gcgaaaaagg aaatcaatgt atcagcacat gcagaaacaa
     2881 ctatacctat agaagtgaaa atcccagcaa aaagctttaa gggacgtgta ttagcgggta
     2941 ttcatgtttc acctaaagaa gaagctgaaa cagaaaatgc tacagaagga gcacaaatca
     3001 aaaatcgtat tgcttataat ctcgcagtcg ttttacaaga aagtcaagag acgatagaac
     3061 cggatctgaa acttttatca ggagatttgg acgaggtcaa cgcaaagcca actgttcagc
     3121 ttcgtttcca aaatcctcag cctaggatta tcagcaattt gatatttacg agcaagatct
     3181 tttatgaaaa tcagctttat attgaaaata cgtctaatga atttttagtt gcaccaaatt
     3241 cgaattttca cttgaatctt gatttagctg gtgataaagc aaaagccggc gattaccgcg
     3301 cagaaatcat agcaaaatca ggcgacagta atgaatggcg atttacacaa aactttacga
     3361 tcaaaaaaga aaaagcacaa aaagtaaatg aaaattccgt ttttgcagta caagaacaat
     3421 ctttcccatg gctttatgca ggattggttg ctgcagttct agtgttatgg ttgattttgc
     3481 tgtttttgtg ggtccacaag aaaaagaaaa aaggagaaac aacatgatga ggtgatttcg
     3541 ttgaaaacat cgatcttatt gggagggatt attgcaagtt tgctgttctt cagctggcaa
     3601 gagccagtcc aagcagatac caaaagttca ggtacgatta cattttatga tctaagtcca
     3661 gttccgcatc caagtggttc agtcaatggc gcatcaaaac agcagacaca gctaccgatt
     3721 tataaagaat ctaatcaaca tggcgtattc ccggagacga atgaagaaaa gatccgtttt
     3781 ctttatttga tcattggtat agaattatgg cttcttgcgt ggttgttgtt atggagaaga
     3841 aaggaaaagg aggatgaaca tgttgctaca aaataaattt tcattttttc gctccttctt
     3901 ttttacttcg ctttttgttt taagtatatt tatcttttca ccttcggcag aagctgttgg
     3961 agcaacgcct cctgccattt cttatgatcc atacacgtat gaaaatatcg tacctccaaa
     4021 tggtggacgt agtgcagtca atggcgggaa gcttgtccaa ctaactgggg ctaaccaatt
     4081 gcctaaattg actgctactt atttgaacga tggttcaaat gatccttgga aaggtggcac
     4141 ggtccgtttt cattgggaaa gccatgtgaa actaacaaat gtacttgatt tggtgaatat
     4201 cactgcacaa ggatttgcat ttgtggcgat catcaatttg ccggaacatg tatcaagcag
     4261 tgatgtatta aaagcaattg actggaacaa cgcctacttg tatattgccg gtgcccccat
     4321 caaggttact agcggtagtt tattatcagc aggaagccat accttgcgtt ttgggatggg
     4381 gtcgtgggat acaaatactc tattggcaga tattattgct attattacgc gaggcgaatt
     4441 caacgtggac gatatcccgc ttcaatttga tgttacagct aatgtcgcga agatgacaga
     4501 aaacggagat aaatatgata cgagtccaaa caaaattttg accaagggta aattacaacc
     4561 ttctatgtct aaaaaggccg ttttttctgt tgatttttat gattcgaata atattatcca
     4621 agatcgtatg ttcaattcga ttttagctcc agctgcaggt agattttatc ctaaattaac
     4681 tggcggaaac ttgaatccag gagcatatgc attttctgca agtgcagaga taaatacgtg
     4741 gaatagttac atctcaacaa gtgattcaac aggaaaatat aatacatttg ctgataccac
     4801 agaatacgtg gatggaaata accagaacct tccaggctct accaatagtc ggacattgac
     4861 gatggggctg actgaaggac gttttgatca gttgccattt agtcgctttg atcgcgtagt
     4921 caactatttt acaggaaaaa acgttacaca aggagcaata ctgactcata ctccagcaag
     4981 cacaagtgga ttgaatcaga cagccaatat cgtgtacagt gggaaagatg cgcaaggcgg
     5041 attgctttct cctgttgctt taaaggtatt ggagcagtac aagctagacg gaacggtttt
     5101 accaacaaac ttaacagccg atagtcagtg gggaaagctc ctttcttctg catcgtacca
     5161 agtcacggcc aattggtcag caactgaact aaaaaagggt tcgatccatt acaggcttta
     5221 ttcgaaagga acccaaaaat tagcgacagg atggacagat caacttttcc aaacgatcca
     5281 atcaaataac gggggtgtaa atacagctac ttctacacta cctgcattgg cttcagggca
     5341 atattatttt gattatcgct tggtggacga tgtacttact aacatgtatc cgtcttttgc
     5401 gtacaaatgg cagtctgaac aagctggaat caccactctt cctcaaataa cagtggctaa
     5461 ttttccatct gtgagtgcag atagtcagct aaaaaacctt agcagacaac ctgagacagg
     5521 agatcccctg tctgctctat caggagatac gattcaagaa aatgtgacat tcacgttaac
     5581 aaaatctggg gatgcgataa ccgacaaaaa aatcacgatc tcacttcctg aaaatactac
     5641 ttatatcaat ggttccttaa aattaaatgg tacaactatc gcagataccg gcattcaaca
     5701 aggagtttct gtcccagcag atctattgag caaaatagga gataccattc atcttactta
     5761 caactatcaa ttaaatactg tagatacttc tgtacaatcc gtcagcatac tgacaaaggc
     5821 agctgtactt tccagcaata ttacattagc agatggtgct aagcttccta acccagttgt
     5881 ccaaacatct gctaaaacaa ttcttgtccc caaacaagaa ttaacattag ttaatgtacc
     5941 agatgatttt acatttggta acgatctccc caaaccgttg aagacaagtt attatgaagc
     6001 aaaaggcgat ttttcttttg atgtgcggga tactcggctt ccaagcacgt ctccttggca
     6061 actgacaggg acattgactt ctttatttaa gaatgatcaa ggtcaagaat taagcggaac
     6121 aaaattatac ttcaatcatt ctggatcaaa acaattgatt cagcaaggtc aaaacacgtt
     6181 gatttatgag agtgatggaa cagctaaagg agaggttctc gttgactttc ccgataccga
     6241 tggtttactc ttagaggtaa atagcagtac gaatgctcaa cctggcgcaa cgtatcaagg
     6301 catggtcaca tgggaactga cagctgggcc aaccagctaa gtgattagaa tttgtatgga
     6361 tctgtgataa ctgtcacaga tcctcttttt caaggatgag aaggaaactt ctgctgttaa
     6421 aatcagaaat gcttggagga gtggacttgg aacttttcag tttgcttgaa aaaaatgaac
     6481 aacggcaaat caaacttttc caacttttat tagaggaaaa aggagagatt tccgttaata
     6541 acgtacttac ccgcttatca gttgatcggg ctaccttgaa agaagatctg ttttatttac
     6601 aagctaactt ggaaaatatc aaacaattgg aaattgttat ccaagaaggg aatattttat
     6661 taatcagaca tggaaatata gcagcagtcg atgtttatta tcattgctac ttgcgctatt
     6721 ctacaaaata tcagatactt ctttatctgt tggaaaatgg ctcatttgat cggcagaaac
     6781 ttgtcatgga acttaatatc agttcggcta ctttgtctcg cagagtgaag gaattaaatg
     6841 attctttaaa tgagtttcat ctgaaaataa aaagcgggcg gctgaccggt ccagaaagtc
     6901 aaatttgtta tttttatttc caattatttt ggtttggacg tccttatctt gaaaacaaaa
     6961 aagaatacag tgatttatca ggaaacactt ttttagatct gctgcaaaaa gagtttagtt
     7021 ttactattac agaagaagga cgattgaaat ttgaaatatg gctttccatt atgaagaagc
     7081 gaatgcgctg tacgaaaaaa tccaaaccag attatagcaa tcttcctgtt ggattgtttc
     7141 aaacgacaca atttttattg gacctccata catttttgac ccgttacttc tttcaatatg
     7201 cctttccttg gaatcaaaat gaaacgttgg ttttttatct tttcatgatc agtaacttta
     7261 cattagatac acataactcg catgtccagg agtttttgat aagcgaaaaa tacaaagacg
     7321 acgtagtagc tcaattgaat cgaacgttta aacaatcgat agaagaatat ttctcttttt
     7381 atgtttttcc agaagagatt tcgcaaaaga ttgcgttgac agtgatgcaa aatcacgctc
     7441 attttgccta ttttagagga tggatgaata tttttggcca agaaggatta aaacagcggt
     7501 taactgaagt agaagataaa caattcctgg ttttatgtga aagattggtc agacaaacat
     7561 ttgctattgt tgattttact gaagtagacg aggaaaatgc ccgctttgaa ttatttgctc
     7621 gctatgcatc tattttgcgg acgctgatca agcagttaga attacgtctg caaattgctt
     7681 atgatcttcc ttatgaaagg ctaatggcgg atatcgtgaa agaaatcatc caggaacaat
     7741 tgctgaatac gatcaaatat gatttagtcg aatacgagaa agaaaagcag tatgatatca
     7801 ttttgactag ccaattgaaa gagtacccgt cacagaagtc cgctaaagtt tttgtattca
     7861 ctagcaacga atataattat gatttcccct atttgaacca atttttaaaa gaatgttatt
     7921 tagaaaaatt gaatttaagg atgaataaaa catgaaaatc gtgatcattg gtgcttccca
     7981 tgcaggcatc acagctgccc taaatctaag aaaacttcaa ccgaaagcag aagttttact
     8041 gatcgatgaa gatcataaag atggactagg ctatgtttca aatggaatca acctttattt
     8101 aaaaggaaag atacgtagtt tagctgaagt tgcccataac atgcggactc ttcaagaaag
     8161 tggggcaaaa ctgatcaccg aatggagagt aactgaactt gatccagaca aacatcagct
     8221 catcctcgca tccaaagaag gaaaaaacga gaccataacc tatgataaat taatcgtagc
     8281 aacaggttcc tctcctgtca ccttgtataa acaaattgaa gctgaaaatg tctacaccta
     8341 taaaaatcta gtgcaatcta agcaagtact tgccgcttta aaagaagcaa aagaagtagt
     8401 gatcttcggt gctggttata ttggattgga attagcagat gctttgagaa ataaaggata
     8461 cattattcat ctagttgatt atatgccgaa tgttttatca agatatttcg ataaggatat
     8521 gatcaacagt tttcaaaatc agctgcaaac taagcaaatc aatttttatc caaatgaatt
     8581 cttgatcgat tggaaaaaat cagaagagaa agtagtatcc gtacaacttc tatctcaagc
     8641 aataaaggca gatatggtca ttttttctgc ccaaactcgt cctaatacga cattgttaaa
     8701 agaaaaagtg gctttgtatg aggatgagac tgtgatagtc aatgaatatc tgcagacaag
     8761 tgatccggac atctatgcga ttggagatat cgtcccagtc agttttgaca aaaataaacg
     8821 gcatctcttt ttacctttag ttactcgagc tgtacatatg gcaagagcag tagcgctaac
     8881 tttgagtggt cagccaaccg cttacgacct cagacaaaag ataacagcag cagtcattat
     8941 agactacttt ttaggaactg tagggctgac agaagatgaa gcgccattct tggaacaaaa
     9001 taccggttcg tgttcaggag aatttgatct atttccacaa tacgatgaag aaaacaaaac
     9061 agtaaatgca aagctgatct accatccgga tacattagaa ataattggcg gacagctgat
     9121 aagccaagaa ttcttattat cagatttgaa cctgcttgcg gatatcgtga aacacaaaac
     9181 tacgattcca cagctagcag ttgaggattt tggatttctg tctgaacaca cctctcgttt
     9241 tcactacctc aatgagttag cttttaaagt actagctaag agagcaaata gcaatagagt
     9301 agataaaaga ctgtgagaaa tatcacggtc ttttattgtg tggggatata ataaatatta
     9361 atcaatcgag aataaaaaat tcatataatg aaatagcgga agaatttttt gagcataata
     9421 ttcgccaaaa atttatcaag tgataaaatt gttgtataaa atgattattt caggaggagg
     9481 agttaaatgt ttttagcatt aaatgaaatt atgcattcaa agttacgtta tgcgttagtt
     9541 gcaggggtga tgtttttgat cgcctacctg gtattttttt tgactgggtt agcttatggc
     9601 ttagctcaag ataatcgaac agcagtcgac aagtgggagg cagattcgat tgtgctttcg
     9661 aaagatgcaa attcaaatct agggatgtcc atgattacta agaagatagc agaagaagtc
     9721 gaaggtggga aagtggcgta tcttgcccag acacctggag tcgtgactag taaagacagt
     9781 acagaagaag gaaaaatcaa tgttagcttc ttcggtatcg ataaaaatca attcatcatg
     9841 cccaatcttg ttgaaggaaa agcttttgat aacgatgatg aggcagtagg cgatattagt
     9901 ttgaaagaag aatatggtct agccgtagga gatacagtta agttatctgg tagtgacaag
     9961 acgtttaaac taactggctt tacggatcat gccaaattca atgtctcacc agttctctat
    10021 acaaccatca atgcttacca agagatccgc ttcgaaaaag aagatacaag tgaaaatgca
    10081 cggattaacg caatagttgt cagaggtaaa atcagtaatt tacctgaaga tttagaacag
    10141 atcaaaattt ctaaattcat caatgaatta cctggatata atgcgcaagt attgactttt
    10201 ggatttatga tcggattttt gatcgttatt gcggctattg tgatcgggat tttcatttat
    10261 gtactgacga tgcaaaagat taatatcttt ggtgtaatga aagcacaagg gatcacagga
    10321 ggatttattg cgcgttctgt agtggcacaa acattcattt tatcatttgt tggtattctt
    10381 cttggtctat taggaacagt gggaacatcc cttgttctac ctgatgcggt tccattccaa
    10441 agcaattggc tgttttttgg cgtcattagc ttgttgatgc tggtagtagc tgtgttaggc
    10501 gcactatttt ctgttcggac aatcgtaaaa atcgatccgt tgaaggcaat tggataagaa
    10561 ggaggaagag catgaaagca atagaattta aagaagtaga aaaaagtttc caagatggcg
    10621 accaagtagt taaagcattg aaagaaacaa ccatgagtat cgataaaggg gaatttgttg
    10681 caatcatcgg tccttctggt tcaggtaaaa gtactttttt gactttggca ggcggattgc
    10741 agacgcctag cagtggctct gtatttatca atgatcaaga attcagtaat caaaaagaaa
    10801 gtgaacgcgt taaactacgt tttaaagaaa ttggctttat tttgcaagct tccaatctgg
    10861 ttccttttct gacagtaaaa aagcagttat tattagtaga taagatcaaa catgaacatc
    10921 gtcaaaaaga agcaaaagaa ttatttgagc agcttggcgt ggataagttg atcaacaagt
    10981 atccagaaga actatctggt ggagaacggc aaagggtcgc aatcgctcgt gcattgtaca
    11041 atgatccgtc cattattcta gcagatgaac caactgctag tttggattca gaaaaggcac
    11101 gagaagtagt cgatatcttg gcaaaagaat caaaagacaa aaataaagca acgatcatgg
    11161 ttactcacga tacacggctg attgacaaat gcgatagaat atttatgatg gaagatggtg
    11221 tattgaaaga aaaagaataa ccaaaaagca ggtggaaaaa tggtccatcg atacaacaaa
    11281 gatacacagc aaaaaatttt gaaagaagcg actgacttgt ttatgtccaa gggatatctc
    11341 ggcacttcaa caagagagat cgcacaaaaa gttgggatca cccaacccaa tctctatcat
    11401 tatttcggtg ataaagaaaa actatatacg gcagttttag aaaaccactt aaaagatgtt
    11461 ggaaaagctt tgagagaaat cgttcaaaca agcgaaacgg gatttcaagt aaccttgaca
    11521 aaaatggcgc agtttttgat tgagacacat ctggtcgatt tgtttctgat gcttcacgat
    11581 cttgaaagca atctttcaaa ggaaacaaga gatcatcttt tctttctatg gaagaaaaat
    11641 tatcgtgaac cttttgaaga aatcttttca gcaaaccaat ctgtgctaag aaatgggatt
    11701 tcaaaagaaa tcgcagcaag gcattttttc ttagtgttag caccttacat cactcggtcg
    11761 aaaagacctg tggaagaacc gctaaatgtg gcgcaattga tcgatttata tttgaatggt
    11821 gtattaggag aagcgtaaag ctgttatata gggagaaaaa tagttaggtg aagaagcaag
    11881 atatgacgaa gatcgttata tcttgttttt tttatatagg tagtttttca taaatacaaa
    11941 tttgttattc tctcatgatt atcagggtat atttttattt attatgtcgg tgttccagga
    12001 agtgttttaa tttaataacg aagaaattaa ttgtttgtag aatgaaggaa actatttatt
    12061 gggtagatga atcaccacga atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa
    12121 aaatcatttt agcagtaatg ttattttcta cggttatatc ttttacagcc ccttttttgc
    12181 gtacttaagc tgattttaag ttttgaccct tagtctggat ctacgaggtg agagtcaaga
    12241 atgaaaaaaa taattggagc ttttgtattt gcaatcatta tcattattat ctgttttcaa
    12301 atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg tcagcagtac gaacagtacg
    12361 gttacaaaga caaaggaatc atttgatgat acagaacaag agacattaat caaaatcgga
    12421 aaaaatgacc gtacactata tacagatgac tcgctaacta ccccacttgc aactataaac
    12481 ggcggagagc tggcggagtt tctttctgaa acaaaaaatg cttatcagat caagacaaat
    12541 gatggctata ctggatattt agccctcgaa gatggaacca aagtcacaaa gaatatacaa
    12601 acaaaaccgg aagagctatc tgatgcagtg attgtattag atcctgggca tggcggaaat
    12661 gatactgggg cattaagcaa tgatgaacaa acagaagaaa aagatataac attaagtaca
    12721 gccataaaag taaaaaaggc gctagaggat gctggagcaa cggtttattt gacccatact
    12781 acagatgaat tagtacagct tggagatata tgcgactaca gtgaagaaaa aaaggcagat
    12841 gtctttatca gtttacacgc agactctacg gaatatgcga atgaagcaac tggaatcacg
    12901 acatattatt actatggaca agaggaaaca ttagcacaga caattgctga ctccttcact
    12961 gatctgcctc tggattcaag aggaatctcc actgggaact atcaagttct tcgagaaaat
    13021 cttcaacctt ctattttaat cgagatgggt tatatgaata atgatagtga tttaaaggaa
    13081 ttagtaacag acagttatca gcagcagatt gctgcaagtt tgacacaagc attgaccact
    13141 tattttcagt aataaataag cccagccaaa caaaaatagc tcctcatatt tttcgtttgg
    13201 ccgggcttat tatcgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgagcc
    13261 tgccgtttaa ctccgcgtca caaggagcaa tttcaaaaaa cagcttctca tgtttgatga
    13321 ctattgtgac ttgtgaccga ggagttggca tgagaacacc gtttattcgg aacaagggac
    13381 tgtgacatga cttttgtcac agttccatta tttttttagc agcttcaaag ctttttctgt
    13441 attggcaaaa tgaagtttgg catatttttc aagcataggc atgccgcctt tctccaatag
    13501 ttttgccgct acttggtcag atttcgttcc agctcctgat ggaagggtga ttccagcttc
    13561 agctgatgct ttgtgtaact cttccaaaaa gctggcacga ctgatgatag aagcagcagc
    13621 aacagctaaa tgatattgtt ctcctttagt aacgaagtac agtttttctt gtaattgatt
    13681 tttttctaaa cgcgcatatt ttttaaaatt tgcttccggc gtaaactgat cgatcaaaat
    13741 cgcttctggt ttttctggct cgattttttt tagcagcaaa tggatcgctt gattgtgcaa
    13801 agcgactttc atccgaacgg cattgtattt tggttggatc tcattgtatt tttctggaga
    13861 taagacaagc agtttatagg ggatggtttt ttttaagatg tcagcaagct ggatgatttg
    13921 gctatctttt aactcttttg aatctctaac accaagttta cgtaattctg caagctgatc
    13981 acgtttcaca taagcagcac atactgttag cggaccaaaa tagctgccat ttccgacctc
    14041 atcactacct aaaacggata attgactgat attttttggc aaagaagagg ctgtttttgc
    14101 tggcttggct tgtttatcct gtgcctcccc ccatttatca gcttctattg ctgcattcgt
    14161 accttgaaac atcacttttc cggatgtata agcggtaatg gtcgtcccgt ctttttttgc
    14221 agcaaagcta gtataaggaa tctgtttatt taacaaataa gagcgatagt atgctttcat
    14281 ttgactaagt tggtcgtttg ttgctttgat aatgacgttg ctcataaaat tctcctcgtt
    14341 tttatacata aaactatacc gcaactcacg acttttcgtt ttctaagaga ttgcgagaca
    14401 tagcattttt ttaaagttta tcatataata gttaagtatg tgaaaaatat ttgtggagaa
    14461 cggtgaaaga accaacttca tggtacacta aataaaactt tcatcaaata ggaggaaatg
    14521 attcgtatgg cgcacgaaaa aacacgctac aaagcagtga ttgccaacca aacatatact
    14581 attatcggac gggaaaccaa gcatcatatg gatatcgtaa cgaagctgat caatgaacag
    14641 ttagcagaat tgaaacaatt atcaccacaa atggataatg aacaagcagc aatcttgatg
    14701 gctgtcaatg cgctttcgga tcaattgaag aaacaagaaa gaatcttgga gctggaagaa
    14761 gaaacagcag aattaaagaa aaaaatgatc aaatttacag agcttgaaaa tcgtgtgaaa
    14821 cgcattgaag caatcgagaa cgaagctcgg gaagtattga aagaaaatgg tcaagcagat
    14881 cataccatta aaaatcatgt ggaagcacaa cagattttaa acgagaaacg aaaaggacaa
    14941 attaaacaaa aatcttctaa ttaagatcct tttatcaaaa taagaaaaat ttaaaaaagt
    15001 tagaagagaa aggaacgtag catgctgagt ttgctgattt tatttttatt gctaattgcg
    15061 tttttttctg gagcacgtcg cgggtttgct ttgcaaatca tattcgcgat tggatatgta
    15121 ttgtcattta ttgctgccca gcatttttac aaaccattag caagtcattt ggaattatac
    15181 attccatacc cagctgtcac cccaacatca aagttagcat tttttgatca ggtatttgct
    15241 tttcacctag atgaagcttt ttatgcagga actgcttttc tgattatttt gctaattggc
    15301 tggctgctta cccgatttgt gggggttttt gtccatggat tgacgtatgt gccgatcttg
    15361 agacaggtag actggattgc aggaggcatc ctgtcactaa tcatggctta cgttactata
    15421 tttttgattt tgcagttact tgcctttgtt ccattagatg tggtccaaaa tcaattcggc
    15481 gagcatagtt tcgctcgttt tatcgttgag agaacgccat ttcttaccaa caagatgtat
    15541 gagctttggt aacgaatatt ttgaattaag gaaaatagtg ctggaaaata tttcgttttt
    15601 tgtacagagt agtttattta agaaggctgc gtagcgtatg tccggcacag ctcttttttc
    15661 atagtgaaag ataaggtgat aaaatgaata accgaatcct tgaaacattg gaatttgaaa
    15721 aagtcaaaca gatggtcaga caatttgtag tgactgcaca aggaaaagaa gaactggcag
    15781 aattggtacc agtgagtgag aaacaaacga tcacaaactg gcttcaagaa actgaggatg
    15841 gattgaaagt gcaacgtctg cgaggcggga tccctatacc taaacttgaa aatatccgtc
    15901 cccatatgaa acggatcgag atcggagcag acttgaatgg agtggagctt gcacaagttt
    15961 ccagagtatt gtctacgaca agcgaattga atcgtttcat tgatgatctt tctgacagtg
    16021 aaatcgaatt tgcacgtttg tatatgtggg cagatcagtt agtcacgatc cctgttttga
    16081 gccgccgttt aaaggaagca atcgatgaag acggacgcgt gactgatgac gcttctcctg
    16141 agttaaagag cataagacag aacattcggc gaagtgaaca agctgttcgt gaacagttag
    16201 atgggatcgt ccgtgggaaa aatgcaaagt atttaagtga tgcgatcatc accatgcgaa
    16261 atgaccgcta cgttatccca gtcaaacaag aataccgcgg tgtatttggc ggtgtagtcc
    16321 acgatcaaag tgcttcagga cagactttat ttattgaacc aaaacaagtg gtggatttga
    16381 ataatcgttt gcgtcaatac caaatcgctg aacgaaatga gattcaacgt attttaagtg
    16441 aactttctgc tgaacttgtt ccccatcgtc aagaaatcat ccacaatgct tatgtgattg
    16501 gtaaaatgga tctgatgaat gcaaaagcac gatttggtaa agaagtaaaa gccatcgtgc
    16561 cgggaatcag tgaagacaat catgtcgtct tgaaacaagc aagacatcca ctgatcgatc
    16621 aggaaaaagt agtttctaat gacatcacaa tcggaaaaga ctatcaagcc atcgtcatta
    16681 caggacctaa cacaggtggg aaaacgatca cgttgaagac tttaggtctg ttacaactga
    16741 tggggcaagc aggattaccg attcctgcag gtgaagaaag tcaaatcggt atttttgagg
    16801 aagtatttgc ggatatagga gacgaacagt cgattgagca aagtctatcg accttttctt
    16861 ctcatatgac gaacactgta gatattttat caaaagtcaa tgaaaaaagt ctggtattat
    16921 ttgacgagct cggtgcagga acagatccgc aagaaggtgc tgctctagca atcgcaatct
    16981 tagatgatct tggtaaaaag tctgcttatg tgatggcaac aacgcattat cctgaattga
    17041 aagtttatgg atacaatcgg gcaaatacga ttaatgccag catggagttc gatgtggata
    17101 cactaagtcc aacgtatcgt ttgctgattg gcgttcctgg acgaagcaat gcgtttgaaa
    17161 tctctagccg attaggtctt gatacagaag ttattgatga agcgaagcag ttgatgaatg
    17221 atgagagcca agatttaaac gaaatgatca cagatttaga gaatcgccgg aaaatggcag
    17281 agaccgaata tctggaaatg cgtcattttg tttctgaggc acaagaactt cacgatgatt
    17341 tgaaagaagc ttacagctat ttctttgaag aacgtgaaaa agaaatggaa aaagcaaaga
    17401 aaaaagcaaa cgaagtcgtg tcagaggcgg aagaaaaagc agaaaaaatc attgctgata
    17461 ttcgtaaaat gcaacagcag attgggcaag gaaatgtgaa agagcatcag ttgatcgatg
    17521 caaagaccca attagcaaat ctacatcaag aagaaacttt gaagaaaaat aaagtattga
    17581 agaaggcgaa agaacaaaaa acgttgaaac cgggagacga agtgttggtt acgacttacg
    17641 gccaaagagg gacgctttta cgtaaaaacg ggaatcagtg gcaagtagaa atcggtatat
    17701 taaaaatgaa tgtgtccgaa gacgagctga cgcctgttgc tcctcaaaaa gagccgacac
    17761 aacgagttat ccatgctgta cgatctgaat ctagtagtca cgtgccgaat caattggacc
    17821 ttcgaggaaa acgatacgaa gaagcgctaa gtgaggtaga tcaatatttg gattctgcca
    17881 ttctagctgg ttatcctcaa gtgactattg tccacggaaa aggaacaggc gcacttcgca
    17941 agggaattac tgattatctt aaaaaccatc ggagcgtcaa aagctttgag tttgcaccgg
    18001 caaaccaagg cggaaatggt gcgacaattg ttaaatttaa gtaaagctta ctaaaggtaa
    18061 gcagatggtt cgctatccaa gtatagaaat gctatactaa tcatagattt taaaaatgga
    18121 ggtctttgat atgtcacaag taattacaga taagacattt aatgaagaaa cagataaagg
    18181 acttgtattg attgattttt gggcaacttg gtgtggtcca tgtcgtatgc aagcaccgat
    18241 tttggatcag ttggaacaag aatatgatga agaagaattc agaattgcta aaatggatgt
    18301 ggatgaaaat ccagaaactc ctcaacaatt cggtatcatg agcattccaa cattaatgtt
    18361 gaaaaaagat ggtcaagtag tagaaaaagc tgttggtgtt cattcaaaag aacaactacg
    18421 tcaaatgatc gatcaatatt tataaaaact tacagcaaaa ataagctgag ggtgggactg
    18481 aatccagttc cgtcctcggt ttttttgcgg ttgagttaag gttattcaaa ttaatatttt
    18541 ttgagtataa ttacgttgtc aggctggatt gtttttttga cagacatgct ataataactt
    18601 taattgaata tgaaggggag taccagatga acgaacgaat caaaaacaaa ttagctttac
    18661 ttcctgatca gcccggctgt tacttgatga aagataaaaa tggaacgatc atttatgtcg
    18721 gcaaagccaa aatattaaaa aatcgtgtcc gctcatattt tactggcagc cataatacga
    18781 aaacggaacg gcttgttagt gaaatcgaag actttgaata catcgttaca gaatcgaata
    18841 tcgaagcatt gctcttagag atcaatctga taaagaaaaa tgatcctaaa tacaacatta
    18901 tgttaaagga tgataaaacc tatcctttcc ttaagataac gaatgaaaaa tatccccgtt
    18961 tggtgattac tcgtaaagta ctaaaagaca aagcactgta ttttggacca tatcccgatg
    19021 tcggtgcggc gaatgagaca aagaagatcc tcgatcgatt atttcctctg agaaaatgca
    19081 aacctagtca gacgaaggag ccttgtcttt actatcattt gggacaatgt ctgtgtcctt
    19141 attatttcga cgtagatccg aaagtctata cgaaaatcgt tgatgaagtg aaaaagtttt
    19201 taaacggagg acacgaaaaa atcgagtcag agatccatca gaaaatggaa aaagctgctg
    19261 aaaacatgga gtttgaaaaa gctgcagaat accgagacca gatccgtgcc attgagacaa
    19321 tcatgactcg ccaaaaaatg acaaatactg atttactgga tcgagatgta tttggttatg
    19381 cgattgacaa aggatggatg tgtgtccaag tgtttttcgt aagacaagga aagctaattg
    19441 aacgagatgt ttcaatgttt cctttctata acgaagcaga agaagatttc ttgacttacc
    19501 tcggtcagtt ttatcaggaa aatgagcatt tcattcctaa agaagtcttg attcctgata
    19561 cgatcgacaa gcccagtgta gaagctttgt tagctacgaa agtgctgcaa ccaaaacgag
    19621 gagaaaagaa aaaattagtt gaattggcga ataagaatgc ctcagttgct ttgaatgaac
    19681 ggtttgatct gattgccaga aaacaggaac ggacgatcgg agcagttgaa cgtttaggaa
    19741 acgcgatgaa tatcccgacc ccaatacgta tcgagtcttt tgataactct aatatcatgg
    19801 ggacgaatcc tgtggctgcg atggtcgtat tcattgatgg aaaaccggca agaaaagaat
    19861 atcggaaata taaaataaaa acagtcaacg gaccggatga ttatgcatca atgagagaag
    19921 tcatctatcg cagatattca cgagtgattc gcgaagggtt gcctttacct gatttgatct
    19981 tgatcgatgg aggaaaagga caagtagatg tggcaaaaga tgtcttagaa aatcaattag
    20041 gaatcgatat tcctattgct ggcatggcaa aaaacgataa acataaaaca aatgagttgc
    20101 tattcggtat agatttggct gtagtaccgc ttgagcgaaa ttcaccagag tttttcttac
    20161 tccagcgaat ccaagatgaa gtccatcgat tcgcaatcac tttccatcgg cagctacgca
    20221 gtaaaaacag ttttgcttcc cgattagatg aaattgaagg gttaggacca aaacgaaaaa
    20281 aaatgctgct taaagagttc aaatcgttga aaaatatcac agcagccacg acagaagaat
    20341 tacaagaaat cggactgccg aaaaatgtcg cacaaaacgt atttgataaa ttacatcagg
    20401 aatgaggaaa gggcacttta caaagaaagt aaagtgtcct atcattttat gtacaacaaa
    20461 aagagcgttt acgttttgtt ggtaaataat aaatcgctta cgtctggtgt ctaaggatat
    20521 atgtgatttc ttgaagaagg agtgaagaaa tgttagaagt caaggatctt gttaaaactt
    20581 ttgggaacta cacagctgtc gatcatgtct ctttccaaat ccccgatgga aaaatcatgg
    20641 ggctaattgg ccaaaacggg gctgggaaga caacgacttt tcgtttgatc ttggattttt
    20701 tgacacaaga ccaaggagaa gtgttatgga atggccatca tcttggggag aaagattatg
    20761 atgtcatcgg ttatcttccg gaggaacggg gactttatcc gaaagtaacg attcaagacc
    20821 aactgcttta ttttgcagaa cttagaggga aatcaagaaa agagattgaa ccaaaaatcg
    20881 atttttggat ggaaaaattt caggtaaaag ggaaaaagac ggataaagta aaatctttgt
    20941 ctaaaggaaa tcagcaaaaa gtccagctga tcgccacgtt aatccatgaa cctaaattga
    21001 tcattttgga tgagccattc agcgggttag atccggtaaa tgctgaattg ctaaaagatg
    21061 gaatattgga attgaaaaaa catggttcat gtgtcatttt ttctagccac aatatggata
    21121 atgtagaaaa aatctgcgat catttgatca tgttgcgaaa cggaaaaatg gttttgaatg
    21181 gaaaagtaca tgagattcgt gaatcatttg gccgtacgaa gttattcttg gaatctggtt
    21241 taagccaaca ggaagtagaa gaaatcgaag gcgtgaaaaa agtgatcctt catgaagaca
    21301 atacgttaga gattacctta gaagatcctg aagctgggaa agaaatcttt actcgtgcga
    21361 ctcagttcgg ctacattcct atgtttaacc agcaaccgcc aactttggaa gagattttca
    21421 aattgaaagc aggtgaaatc gatgcgtaag ttttggatta ttaccaaaga tgtctattta
    21481 aaaaatgtta agtcgatttc tttcttgatc atgatattag tcccttttat catgctgggt
    21541 atcatttatg tagcaggaaa ctttgctcaa caaaatagtg atgtcaataa aatcggcgta
    21601 ttggctgata atgagcaaat cagtcagcaa atgagccaag tcaaaacaga tgattttgag
    21661 ttcaaagcag ttgattctga agaaaaagca caaaaagagc tttcggaaga aaaaatcgat
    21721 gcgtatttag tagtctcaac tgagaatgat aagataaatg ggatgcttta tagcgaaaat
    21781 tcattaggac aaagtacaca actactgata caacaacaac tgactggctt gcaatcgatg
    21841 cttcgagcaa gtcaattagg catttcacca gaagaagtag ctagcttgag ccaacaagca
    21901 agtttgactc gacaaaaagt caattttgat tcaaaaggaa aaatgacagt tggagaagat
    21961 aactcgacag tccaatatgt cgtaagttat gtagcaacga ttgtcttgtt cattattatt
    22021 ttgacgtatg cgcagatcat tgcacaagag atcgcttctg aaaaaggcac gcggatcatg
    22081 gaagtgatcc tgtccagtac gactgcacaa aagcattttt acggaaaact aacaggtgtt
    22141 ttacttgttg cattaacgca aatggcatta tacggtatta tttttggtat aggatacagt
    22201 caattcaaag atatggaaat ggtcaaaaat atattagacg gtatctcatt ggataacttg
    22261 attggaccat tcttgtggta ttctcttcta ttcatgatct ttggcatttt gattttttct
    22321 gttttagcag ctttatgtgg ctctttggtc aacaaagcag aagatacagc aaaagcaatt
    22381 ttgccagtga cttatctatc tctaggcgga tatatgcttg gattgatact aggagcttca
    22441 gatccaaaca atatcgttat tcggatcact tcgtatattc cattcttatc ttcatatatt
    22501 atgccggttc gcttagcaaa tgagacagtc ggtatgggtg gagcattgat ttccttgttg
    22561 gtacttgtag ccgtaacatt cttattgatg atcttgtctg caaacatgta caaatcgaat
    22621 gtactcgtgt atagcgaagg cggattatgg agttcattga aacaatcgat ttcaattatg
    22681 agaaatgaac gaaaaaaagc ataaaatata gaaaaatata gaaggaagct ggacatttat
    22741 tgttcccagc ttcttttttg tatagaccta tttctatttt tttattatat tggatatata
    22801 tattcattga aaatagagca taacaacttt ataatgttga gtataccaat ttttggaggc
    22861 gttttaatgg accgctattt agatttactt tcagagaagt tccctacaac ggaagatgtc
    22921 atcacggaaa tcatcaatct cgaagcaatt ttgcagcttc caaaaggaac agagttattt
    22981 ttgagcgata tacatggcga atttcctgct tttgatcata ttctgcgtat cggttcagga
    23041 aatcttaaag aaaagatcaa agaccttttc tctgaacaaa tgacggaaac ggagatcagc
    23101 caattcacta tttttacggc ttatccagaa tatgtgttga cgactgattg gtacaaaaaa
    23161 caagataaat cccagctgat ccatcgtttg atcgatcttt tacggtttac gacagtcaaa
    23221 tacactcgat caaaagttcg aaaaggattg ccgaaagaat atagctatat cattgaggaa
    23281 ctcttgtata tcgatgatcg tatcaacggg aaaaaagact atgtaaaaaa aatcattgaa
    23341 caacttgtct tgatgaagga agaagaaaaa tttttaacaa aacttgcgca gacgatccaa
    23401 aaatctgtca tcgatcattt gcatatcgtg ggtgatattt ttgatcgagg aactcaagca
    23461 gccaaagtaa tggatcgaat catggatttt tcctctgtag atatccagtg ggggaatcat
    23521 gatattttgt ggattggtgc gtattgcggc tctgaggcat gtttattgac gcttttacgg
    23581 attgccgcac gctacaacta tttatttgaa ttagagaaag aatatggatt aaatttacgt
    23641 ccattatttt cttttgccca tcaaacctat cagaagaatc cagtatttac ccctaaaaac
    23701 aggaaagatc tatctgaaag agagcaagaa gaattagaaa agatccatca agcactttct
    23761 atcctccaat tcaaatcaga acatcagttg ttgaatcgac gacctgaatt taaaatggac
    23821 gatcgtaaat tgttggaaaa aatcaactat gaggaatcaa cgatcgtttt agaaggtcag
    23881 ctctatgagt tgaaagggac ttgttttcaa acgatccagc cagatgatcc taaaaaattc
    23941 ttggaagagg aacaaaaagt gatcgatagt ttgatgtatt cattccagca ctcgttaaga
    24001 atgcaaaaac acatgacgtt tttgatcgaa aaagggggaa tgtatctcac gaacaatcag
    24061 catgtactgt tccatggctg tatacctgta gatgaaaagg ggcagctctt agcatttgaa
    24121 ttagaccaat cttatcgcgg aaaagaactg ctgggatttt ttgaaaaaca gataactgaa
    24181 agcgcaaaag atttgactgc aaatgaagat ttggcgaccg atttgatttg gtatgcgtgg
    24241 agcgggccat tttcgccttt attcggcaag agtaaaatgg caacttttga gcgctatttt
    24301 ctcacagagc ctcatacgca cgtcgaaaaa agcaatccct attatcactt gcgagatgaa
    24361 gagtggttca gtgaaaaaat tttggcagaa ttcgatgcaa aagaagaagg ttcagcaatc
    24421 atcaatggac acacgccagt aaaagtaaaa aaaggagagt caccgatcaa agcaaacgga
    24481 aaagcatttg ttattgacgg aggtctatcc aaagcttatc aaaaaacaac cgggattgct
    24541 ggctactcat tactatgcaa ttcttacggt ttccaaatcg tgacacatta tccgttcttg
    24601 cctattgaac aacaatttgc aaaaaaatct gatcaaacga atgtaaaaaa agtgatcgag
    24661 cagcagctcc cacgcaaact taatcggaat acgacaaaag gaatggaatt acagcaacag
    24721 attactcaac tccaaaggct gttggattat cgaaaggacg attgagaaaa atgctagcca
    24781 acattgctca atagactaaa aagcgactgc aacaaaaggc gtgtcgcagt ctccttcgga
    24841 attagctcaa aaaacggtaa aatattagaa gctatttttc tgtttatttc tctaatactc
    24901 aggtcagggc gctgatttca cgacctcttc cttaaacggt gttctcctgc caactcctcg
    24961 gtcacaagtc acaatatcca acaaacatga gaagctgttt tttgaaattg ctccttgtga
    25021 ctcagagttg aacggcaggc tcacgaccta atcagaaaaa cgattaaaat tcattcacaa
    25081 agaaatcgaa taaacgctgt tcagatgaat ctacacgatg agaaagttcc ggatgccatt
    25141 ggacaccaag gattttgtgc atattatctt tactttggac tgcttcaatg agtccatcgt
    25201 tggaaaaggc gatggctttt aacaaaggag aaagatcttt gactgcctga tgatgatagg
    25261 aattcacaaa gtatgaatca gggagaaggt cacttaataa actgtccgaa acgattttga
    25321 cttcgtgagt tgcaaactgc ggggcagtgg gctgttgttc gtgttttacg gaccattttg
    25381 gatataatga aagatcttga taaagtgttc cctccaacac gacattcaat agttgaagtc
    25441 ctcggcagac tgcaaaaatc ggttttcttt gtttcaatgc ttccaagatc aatgcctgtt
    25501 cgaattggtc tcgttgaata tttgtttccg tcagttttgg atgagggtct tccatgtaaa
    25561 gttgcggact aacatcctga ccgcctgcaa gaatgagttt gtcgattttc tcaattagtt
    25621 gaggcgctga atctggagac atgactggca gaataagagg taatccttga gcatctaaaa
    25681 ctgcatccac aaatccctgc ggtgtatagc ttacttgatt tccttggaaa gtatctgtcg
    25741 ctcgaatcag ttgattcccg gcaatgccga tgattggttt catttttatt ccccctaaag
    25801 tagactaatt gtaacaagga tttaaaaaaa tttaaactat gtaagacttg cccctatact
    25861 tatctgttct tgctgtgtta ctatctgtaa agagaatttt cgtgtgaaat tcatgacgat
    25921 agttgaaaat attgcttaat ttgttataat aaaaagtaag cagaaataaa aaggttgggc
    25981 gtaagcttca aggatctagt tcttcaaggg gtgagaagga cattttattg aaggggcctg
    26041 cctatactaa gttatcttta gaggtacata ctttcaatga aaaaagaaag catgtacctc
    26101 ttttttaaat cgaaaggaag tgaagcaagt gttagaagct ttggaacaaa tccgaaaagc
    26161 agaagaagaa aatgagaagc gtgctcgaac cctacggcag cagttgaaga cctatgcaga
    26221 agaaaaagag caagaattgg agataatcca aacgaacagt cggcaacagc tgaaacagtt
    26281 ggtagaagaa aaagaacaat tcgaaaagca agaattagaa aaagaaaaaa tgaaattgaa
    26341 agcagaagca gacaaggtgc aagcaacttt gaaagaccaa tatcaaaagc atcagagtca
    26401 agcaatcggt gcaatcattg aaagggtgaa agaaacttat ggccgtcact aagatggaaa
    26461 aagtgaccct catttcagat aaaagaaatc aggagaggat tttacaagca gtacaaggaa
    26521 tccaaaatgt tgaaatccgg gatctgtatc aaaatagaac aaacaaccag tgggtagaaa
    26581 aatattttcc agataccacg attattgata aagaagcaaa attaagcgct ttaggcaatc
    26641 ggctgaaaga catccaagaa gctatccaat tcatacaaca ccacggcgat agccgtcaaa
    26701 agaaactcca tttgaaacgt caagaacttt ccttacaaga cttggaagca aattattccg
    26761 aagaaaattt catgaataaa ttagaagaag tccttgattt aaaagaaaag tgggaacagc
    26821 taactgaaaa acgtgatcgt ttagaagaag aggaagaatg gcttcttcat tggcaacagc
    26881 tggatattgc tccaagtatg tatgcaagta agacgaccat ttttgagatg gggactgtga
    26941 gtgcagcaaa ttttgaaagt ttcaaggaag aactaagtaa gcttgatgac gtttatatgg
    27001 aagaaataga ttatacgcca aaacaagttt atctttcgta tgttgcttta aaaaaatcag
    27061 ttgctgctat agaagaaatt gctgggaaat acgggttcaa taaagaaacg tatccttatg
    27121 aacaaaatcc taaagaaaaa ttgcaagaaa ccaaacagca attatccatt gttcacgagc
    27181 agcaaaaaaa gataacgaca gcgttaggtc agtgcagcgg ctatatccaa gaatttgaat
    27241 gggtagaaga agtcacactt gccgttgcag aacgggaaaa agtaaaagaa cggtttatcc
    27301 atgccactta tctgatcgtt ttgcaaggtt gggtagatgc agaagaaaaa cagaatttga
    27361 tgcatgtgtt aaacgaaact gtatcagaag aagatatata cgttagtttc gaaacaccaa
    27421 actcagaaga gattcagcag gaagtcccta ctaaattgaa aaaccatccg cttatcgaac
    27481 cttttgaatt gctgactgaa atgtacagtc tgccaaaata tgaagaagta gatccaacgc
    27541 cttggatgac accattttat cttgtcttct tcgggatgat ggtcgcagat gttggttacg
    27601 gattgctgat gctcattggg acaatactag ctcaaaaact atttgtttta ccccgcggga
    27661 tgaaacgttt cgtaaaattc tttgagattc tctcggtacc atcgatcatc tggggattga
    27721 tctacagttc atttttcgga caggcattac caaaagaact atttggtatc cgcttgcctt
    27781 tcccaatcct atctacgacg gatgacgtca atacgatttt gattttatcc gtgattttcg
    27841 gtctgataca gatcttggtt ggattattcg tggcagcaaa ggaaaatatc aggaggaaag
    27901 attacttgag tgctgtcagt gatggttttg cctggcaagg gatcctgatt gggatcgtta
    27961 ttgcattgat tggtgtcatg ttgataaaaa atacagcatt tgtttatctt ggcgcggggc
    28021 tatcgatcat tgctgcttta tgtatcttaa tcgtgccgat tatccaatcg ccgtctaaag
    28081 tcaagggtgc tgccaaagga gcgtataatc tttacggatt gactggttat atcggtgatt
    28141 tagtcagtta tacacgtttg atggcactag gtatttcagg cggaagtatt ggcgcggcat
    28201 tcaatatgct agttgctttc atgccgccag ccgctcgatt cagtgtaggg atccttttga
    28261 tcatcgcttt acacgcactg aatatgtttt tgacactgtt aagtgcctat gtccatggtg
    28321 cacgattgca gtatgtcgaa ttttttggaa aattttatac aggaggcgga cgtgcctttg
    28381 acccgctgaa aacggcggaa aaatacgtca acatcaatca taagaaaaaa aacaataaat
    28441 aattggagga tttttataca atgatggatt acttaattag tcaaaatggt ggaatggtat
    28501 ttgcagtatt agcgatggca acagcgacga ttttttcagg gattggttca gctaaaggtg
    28561 ttggaatgac aggtgaggca gcagcagcgt taacaacaag tcaacccgaa aaattcggac
    28621 aggctttgat tctgcaattg cttccaggta cgcaagggtt gtatggattc gttatcgcct
    28681 ttttgatttt tatcaactta ggaaatgata tgtcagttgt acaaggattg aactttctag
    28741 gcgcttctct gccgattgct tttacaggtt tgttttcagg tatcgcccaa gggaaagttg
    28801 cagcagcagg tatccaaatt ttagcgaaaa aacctgaaca tgctacaaaa ggaatcattt
    28861 ttgctgcgat ggttgaaaca tatgcgattt taggtttcgt tatttccttc ctattagtat
    28921 tgaatgccta agcataaagg aggaaaagca gatgaacgcc attgaaaaaa tcatctctca
    28981 gatgaatgaa gcagcagagc aagaaagagc ggcgttagag caagaagagc gaatgaagat
    29041 cgatcaaaac tttgaacaaa aacgtacgca aatcgaaaca gaacatcaaa aacaaaaaga
    29101 aaaacaaatc gaattacttg aaaaaaaata ccgtcagctt aggaatcgcc aacaagtaga
    29161 agtacgacaa gaaaatttga atgcgaagca ggaattttta cgacgattgt ttgcagatgc
    29221 aatagctgaa atggagaatt gggatgaatc tgaacaaatc cagtttatca aaaattcatt
    29281 gtattcatta cctttgacag gcaaagtaac attcattgct ggagaaaaat cagcagctta
    29341 tctttctcag acattgctgg atgaatggaa caatgaactt ccctttatga tggttttaag
    29401 tgatgaaact gttgctgatc aagcgggttt tctgataaac gatcaaggag ttcagtataa
    29461 ttttcttttc agcagccttg tacaagatat ccaaggaacg atgagttttg agattgccaa
    29521 tcaattattc gaataaggag ggatcagtgt atggaatacc acgaactaaa tcccttgatt
    29581 cgcggaagag agcttgaact tttgtccaat gagacatttg aacggatgat tcaaacaaat
    29641 tctgtcgaag cacttggtga aatattgaaa tccacgattt acagtccata tatttatgac
    29701 ggatatgaat atgacttcga acagaatctt ttaaaggagc agagccgtct atttcgctgg
    29761 ttaaaggaat cagcaccgga gcctgaaatc gtttggatct atacgatgcg ctatactttc
    29821 cataatctga aggttttgac aaaagccgag atgacaaatc aaaatctgga ccatttgtat
    29881 atcgacgatg gattttattc attagaaacc ttgaaggatg ccattcatac gcaagtttca
    29941 aatgaactcc ctgaaagcat tatggacagt atacgagaag tacgtgatta ttttgaagag
    30001 tctaccattc ttcagggaat tgacgtgatt tatgatcgac agtttttgac agagcagcga
    30061 cggcttgccg ataagctagg atatcaagaa ttgctgacag aagtcattgc ttttattgac
    30121 ttgaccaata tcacaacgat ggctcgagga atattgcaga aaagaacata tgggttcatg
    30181 acgacggtac tttccagctc gggaagtatc gaaaaagaag tatttttgaa ctttgtagat
    30241 aaagatatgg aagattacac gaattttcta ttagagacag attatgctga ttttctgcgc
    30301 cctgtcatcc gtgagggaaa tcttgatctc gtaaagcttg aacagttgaa agatgattat
    30361 ctaagttcat tgtatcaaga agcacaaacc caggctttcg gaccactgcc actactagcc
    30421 tttttgaacg caaaagaggt agaaagcaaa aaccttcgtt tactggttat cggcaaaaga
    30481 agccatttcg aaccagaaac aatcagaaag cgggtgagac aagtctatga cgcataagat
    30541 cggtgtagtt ggcgacaaag attctgtttt gccattcaaa ttgtttggct ttgatgttcg
    30601 ttatggtaca accaagcaaa ccgttcgtca ggcaattgaa gaaatggcaa agaatgagta
    30661 tggcgtgatt tatgtcacag agcaatgtgc agcattgagc ccagaaacga tcgaacgtta
    30721 caaggaacaa atgatacctg ctatcgtatt gattcccaat catcaaggat cattagggat
    30781 agggctggaa gaaattcaaa aaaacgtaga aaaagcagta ggtcaaaata tcttatagta
    30841 tgaaaaggag atagaaattt tgcaaattgg aaaaatcata aaagtctccg gacctcttgt
    30901 aatggctgag aacatgtctg atgccagtat ccaagacatg tgtctagtgg gagatctagg
    30961 agttatcgga gaaattatcg agatgcgtgg cgatgtggcg tctattcaag tatatgaaga
    31021 gacttctgga attggtccag gcgaaccggt tcgctctact ggtgaagctt tatctgttga
    31081 attaggacca gggatcattt cacaaatgtt tgacggaatc caacggccat tagatacctt
    31141 tatggaaatc acacaaagta atttcttagg gcgcggagtt caattgcccg cattggacca
    31201 tgagaaaaaa tggtggttcg aaccaacagt agaagccgga gaaacggtga gtgctggaga
    31261 tgttatcggg atcgtagaag aaacaaaagt tataaaacat aaaatcatgg tgccaaatgg
    31321 gataaaagga acgatcaaaa caatcgaaaa aggttcattc actattgatg aaacagtatg
    31381 tgtcattgaa accgaaaatg gtgacaaaaa tttgacgatg cttcaaaaat ggccagtaag
    31441 acgtagtcgg ccaatcaaag aaaaattaaa cccggatgca cccatgatca ctggacagcg
    31501 agtgattgat acattcttcc cagtgacaaa aggaggagca gcagctgtac ctggtccttt
    31561 tggtgcaggg aaaacagtcg ttcagcatca aattgcaaaa tgggccgatg tggacatggt
    31621 tgtctatgtg ggatgtggcg agcgtggaaa tgagatgacg gatgttttaa atgaattccc
    31681 tgaattgatt gaccctaata caggagaatc gttgatggaa cgtacggtat tgattgctaa
    31741 tacatcaaac atgccagtag cagctcgaga agcttctatc tatacgggaa tcacaattgc
    31801 ggaatatttc cgtgacatgg gttatgacgt tgctatcatg gcagactcca cttcacgatg
    31861 ggcagaagca cttcgtgaga tgagcggacg tttggaagaa atgcctggtg atgaaggtta
    31921 tccagcttat cttggttcac ggcttgcgga atactatgaa cgatctggaa gagtcattgc
    31981 attaggatca gaaggtcgcg aaggaagtat aaccgcaatc agtgctgtat ccccgtctgg
    32041 aggggatatt tcggaaccag tcacacaaaa tacattgcgg gtcgtaaaag tattctgggg
    32101 tcttgattcc agtttagccc aaaaaagaca ttttccttcg atcgattgga tacaaagcta
    32161 ttctctttat tcaacagaag tagggaagta tatggatcat atccttcaag ctgactgggc
    32221 atccatggtc accgagggca tgcgtatttt gcaggaagaa cagcagctga atgaaatcgt
    32281 gcgtttggta gggattgatt ccctttcaga taatgaccgt ctgacattag aagtggcaaa
    32341 atccattcgt gaagattatt tgcagcaaaa tgcttttgat gatgtcgata cattcacttc
    32401 aagagagaaa caattcaaaa tgttgaagat gatcttgacc tttggtgaag aaggacgccg
    32461 tgcgttgtcg ttaggtgctt atttaagtga gatcatggac ggtaccgtat cgattcgtga
    32521 aaggatcgga cgaagcaaat atatcccgga aacagaatta gaaaaactag atcaaattat
    32581 tggtgaaatc aagcaaacga tgcaaacgat cgtttcagaa ggaggcatga gcaatgatta
    32641 aagaatatcg tacgatcggt gaagtcgtcg gtccattgat ggcggttgaa aaagttgctg
    32701 gtgtcaaata tgaagaatta atcgaagtcc gtatgcaaaa tggcgagatt cgtcgtggac
    32761 aagtattgga agtccaagaa gataaagcaa tggtccaaat attcgaagga acaagtggta
    32821 tcaatttacg tgactcttct gtacgatttt taggccatcc tttagaacta ggtgtatcgg
    32881 aagacatgat cggtcgagta ttcgatggtt tgggacgacc gaaagataac ggaccagaga
    32941 tcctgcctga aaagcatttg gatattaacg gagaagtaat caatccagta gctcgggatt
    33001 atccagatga gtttatccaa acaggtatct ctgcaatcga tcatttgaat acattagttc
    33061 gtgggcaaaa attaccagta ttttctggct ctggtcttcc tcataaagaa ttagctgctc
    33121 agatcgcgcg acaagcaacg gtactcgatt catcagatga tttcgccgta gtctttgctg
    33181 ctattggaat cacttttgaa gaagctgaat ttttcatgga agattttcgt caaacgggcg
    33241 caatcgaccg ttctgtcatg tttatgaatt tagcaaacga ccctgcaatc gaacgtatcg
    33301 ctactcctcg tatggctttg actgctgcag aatatcttgc ttatgaaaag ggaatgcatg
    33361 ttttggttat catgacagac atgaccaact atgcagaggc attgcgagag atttctgcag
    33421 cacggcgaga agttccagga agaagaggat atcctgggta tctttacacg aacttagcta
    33481 ctttattcga acgagcagga agaatccgcg gactaaaagg atcggttact caaattccga
    33541 ttctaacaat gccagaagac gataaaactc atccaatccc tgacttgact ggttatatta
    33601 cagaaggaca gatcatcttg actagagagc tttacaagag tgggattcag ccaccaattg
    33661 atgtactccc gtctctttcg cggttaaaag ataagggaac tggacccgga aagacaagag
    33721 aagaccatgc agctacgatg aatcagcttt ttgcagccta tgcacaagga aaacaggcaa
    33781 aagaattagc cgtagtttta ggtgaatctg ctttatctga tgtagacaag atctatgcga
    33841 agtttgctga gcggtttgaa aaagaatatg tcaatcaagg attctacacg aaccgttcga
    33901 ttactgaaac acttgatcta gggtgggaac tgctatcgat gttgccaaga accgaattaa
    33961 aacgaatcaa agacgacatg ctggacaaat atctgccaga agggaagtga gttctgatgg
    34021 cacgattgaa tgtgaatccg acccgcatgg aattgactcg attgaaaaaa cagttgacaa
    34081 ctgccacaag agggcataaa ttattaaaag ataagcaaga tgaactgatg cgtcaattca
    34141 tcctgttgat tcgaaagaac aaccagctgc gtcaagaaat ggaagagtct atgacagaag
    34201 caatgtctga ttttgtattg gcaaatgctt cgatcacgga acctttcatc gaagaattat
    34261 ttgttttgcc ggcggaaaac gttgatttgt ctatcgtaga caaaaatatc atgagtgtaa
    34321 aagtacctgt gatgaatttt gaatatgatg aacagctgac agacacacct ttggaatacg
    34381 ggtatttgaa ttccagtgcc gaactggatc ggtcaatcga tcggtttact accctgcttc
    34441 caaaactttt ggaactgaca gaaatcgaaa aaacttgtca actgatggcg agtgaaatcg
    34501 aaaagacacg tcggcgagtc aatgcattag aatatatgac gatccctcaa ttagaagaaa
    34561 cgatctatta tattcgtatg aagctggaag aaaacgagcg cgcagaagta acaaggttga
    34621 tcaaagtaaa aaatatgggt accactgact aagtggtatc tttttttaaa agaggaaaac
    34681 gatgattaaa caaagcaaac aaataaagaa acgtttgccg gctgttcagc ttctcgcatt
    34741 aggatttttt ctcctgattt tagttggcgg cggcctactt accctaccat tttttagtaa
    34801 tagtgggcat gggacaaatt ttatcgatgc gcttttcact gcaacttctg ctgtctgtgt
    34861 aacgggattg acaacattga atacagcaga gcattggaac agtgctggtc aatttttgat
    34921 catgcttttg attgagatcg gcggtcttgg ttttatgatg gttccgattc tatgctttgc
    34981 attgataaag aagaaagtca gtctcagcac acgtattata ttaaaagaag cgctgaatct
    35041 agaagaagta tcaggtgtga tgaagctcat gctttacatc ctaaaattgg cctttgttat
    35101 ccaattgatt ggtacggttg ctttatcttt tgtatttgtg cctgaatttg gctggttgaa
    35161 aggtatctgg tacagtttgt tccatgcaat ctctagtttt tgtaatgcag ggtttgattt
    35221 gttaggagac agtttagcca atcaccagca aaatgtttat ttgatcatgg tggtatctgc
    35281 tttgatcatt tctggtgggt taggcttcat cgtatggcgt gatttactgg aatatcatca
    35341 gaaaaagaag atgacgattc attcaaaaat cgcattaacc gttacaggta cccttttatt
    35401 aggcggattc gttgtgttct ttttcacaga gcgcaatggg gcaacgctag tggaagggaa
    35461 tatgtttcaa cgattggcaa acaccttttt tatgagtgtg acacctagaa cggctggata
    35521 ttattcagtt gattacctcc atatgagcca tgcaggtctg attttaacga tgtttttgat
    35581 gtacatcggc ggaacatctg ggtcgacggc cggcggattg aaaacaacta cgttaggtgt
    35641 gttgctgatc cagatgcgtt cgatgttgaa aggacgtacg agagcggaag catttggccg
    35701 taccatccga caaggcgcag tgttgcgggc acttacttta ttttttgtta cgttgacgtt
    35761 gtgtgtagtc gccatcatga ttttatcggt gactgaaacg atacctaaaa cttctggcat
    35821 cgaatatatt gcttttgagg tcttctctgc gtttggtaca gtaggtctta cgatgggatt
    35881 gacgccagac ttgactgcaa tagggaaatt gatcattata gcgcttatgt acattggacg
    35941 agttggtgta ttgactgtgg tcttctctct gatgacaaaa gcacaaagaa cacaatcaaa
    36001 atacaaatat ccagaagaaa gtgtcatgat tggataattt catcttctga cagaaaccta
    36061 gtgatttagt aaaataagaa aataaaataa tttttcttgc ttcttggata ctttaagtgc
    36121 aaaatgttcg tttatttttt gaggcactac ccgttgttga acctcgggta gtgcctcttt
    36181 tttattgaaa ggaatcaata agatttgatt cctttattta tcaataggtt ataattcata
    36241 tttttttatt ttttctttta cgatacttag tccagaagct aaaatttcaa ggttattaga
    36301 ggatagggaa attcgaacga atgcatctgg ttgcgcacgt gtagtaaagc gtctggaatg
    36361 ataaagtcgg ataccgttag caagaaagtc attctcaatt atcttagggt caatctcagg
    36421 atcaactgga agaattcgat agtaagggac acgattcgtt ggtttttcta ctttgaaaag
    36481 agaatcaaaa agctgattgg ctctttttgt aagacttaat ttagtttcta agatcctatt
    36541 tgcttcattt gaatggagga tttgagtgat gacttccaca tctaatccag aagttttcac
    36601 atttatatta aaaattgctt gttctatctt gctttggaca tgtactggaa ctacaagata
    36661 agcaactcgc aatccagaac aaataaattt tgtcattcca gctaagtaga ttgtttggtt
    36721 aggtaataaa ttttggaaag gaggaatgat cgtatgttga tagtaagttg ttaaaaatga
    36781 atggatatca tcttcaataa cccatagacc ttctttttca atcacttcgg ctaattgctc
    36841 tcgtcttttg tgagagattt gaaaccctat aggattatta caagaaggca taagaaagac
    36901 accatgaatc ttcttttttc tacattcctg aatcaatata tcaggtcgca ttccttgctc
    36961 gtcaaaatca attggaacga tctctaaatg atataactga gccaattcaa tgaaattagc
    37021 gtatgtatat cggtctgttg ctattctatc acctggagag aaaagtgcag aaagtgtgat
    37081 agccaatcca ttttgaacac cggaaacaat agatagagat tctaaggacg gaatgactcc
    37141 ttgagttttt aaccaatttg ctgcagtagt caattgaaaa tttgtgcctt tgggatcttg
    37201 gtaattaagc aaaccaacaa gatttgggtt attactagtt gattgaataa acgggacaac
    37261 gaatttatta cattcttcaa aagaactaac cagaccaaaa tcaatgatat gttcggttac
    37321 ttcattggta gagatggtga tggactcaaa gacattggga gaaataaatg tgccacttcc
    37381 aatattcgta tacagcaaac ctttctcaat tccatattta taagccttgc caacggtagt
    37441 aaaatttaaa tctaaataat cagctagctc tctttgggag ggtaactgag tgtttttctg
    37501 taatttattc tttaaaatat cttgttctaa ttgttgaatc aacgatcgat aataaggacg
    37561 cttcaatttt tgtttgtctg gtcgccaagt taacgggtaa ttgtcataag aattaatagg
    37621 cattttctcc actccttaaa attgtatacc atacaatttt accattgatt gtattgattt
    37681 tagaagttaa tcttttgtga ggagggaatt gtctatgaaa attattaatt ttgaggaagt
    37741 agtagcttct tttagttttg aagaagcgat tcaagtaatg aaaaaatgtt tttttgatta
    37801 tgaaagtggt aaaatcagcc aaaatcctcg attggtggaa attttacctg acggaaaagg
    37861 caaaaatatg tttgcgatga tgcctgctta tttgggggag aacagatatt ttggcacaaa
    37921 agtcattacc gcttttccgg ataatcatca gtatcattta ccttctcatt taggagagat
    37981 tttcttgttt gatgctcaaa acggactacc caaagcattg ataaacgcaa atgcagtcac
    38041 ctggattcga acagctgctg tatctgcgct ggcaacagat tacttagcaa atccgcatgc
    38101 agaaacactt gctttaattg gagctggtca acaagcattg tcgcatttga ctgcgatttc
    38161 taccattcgt cccatcacta cagtgtttgt ttacgatatt ttagaagaaa gacgaaatac
    38221 ttttatcgct aaagctaaaa aacagtatcc taaaataacg tttataaatg caatatcagt
    38281 agaagatgca gtacgtccag cggaaatcat ttgtacgttg acctctagta aagaggcatt
    38341 tcttcaaaaa gagtgggtaa gggaagacgc acatatcaat gctgtgggca catttactcc
    38401 tgatacaaga gagataagta catcgttgat ggaaacttct caaatctatg tagatgatta
    38461 ctcagctgcc ttaacagaaa gcggggacct gcttatagcc atggcagaag gagatttaga
    38521 ggaatcttgt attgttggta cattaggcga attagtttct gagaaaaaaa cattttataa
    38581 aggagaatcg aggccgacga ttttcgacgc agttggtcta gctattgaag atctttgttg
    38641 tgcagaatat atttataaca aattagagaa aggggcttta aaatgaactt agattattac
    38701 gtagttgatg catttgcaga tgaggtgttc aaaggaaatc cagcagcggt ttatgtgcta
    38761 gaagaatggc tgccagaagg tacgatgcaa aaaattgcca ttgaaaacaa tttatctgaa
    38821 acagcattta cagtgaagaa aaaccaagaa tttgagctta gatggtttac gccagatcga
    38881 gaaatcgatc tttgtggtca cgcgacatta gcaacagcat ttgttttatt taactattac
    38941 aaaatacctg atgaaacgat taaattttct actcaaagcg gtaatctatt tgtgacaaga
    39001 attaaagatt attattacat ggattttcct agtattatgc cgaaaaaagt gccgatattg
    39061 tctgaatatg aagaagcgat tggggctaag attaaagaag cctacttagc acgtgattta
    39121 tttttcgtat tagaagatga aaaaacggtg gctaaattgc agccagattt tacagctatc
    39181 aaaaattttg aattaggaat aggagtcatc gtgacagcag aaagtgtgca aaaagacttt
    39241 gtttctagaa ccttctttcc aaaattaaca atcaatgaag atccagtatg tggatcagca
    39301 cattctaatc ttattcctta ttgggcagaa agaatgaata aaacaaaatt gtcagcttat
    39361 caaatgtcat caaggggcgg ggatctagaa tgtgaattaa aagaagatcg tgtgataata
    39421 ggaggaaaag cagttttgtt ttctaaaggt acctcgttta tttgatttct ttcaaatgaa
    39481 ttaaaaataa attggtggag agtggcaacc ttatagctac aatggttgtt aaaaaaagac
    39541 gcaaactttt tcaatttaat gaaaagtttg cgtctcattt taattattgg tgtttgtttt
    39601 ttattcaatc aagagtaaaa tgccccatac taactatcct cggatgactt cgattttata
    39661 accatcaggg tcaatgacaa agtagtaaga aggcgcagtg ccaggtaatc cttttaagtc
    39721 agtaatctca agaccggcag ctttatgttg ctcgtgtaat ttttctagat catcagctgc
    39781 tatagcgata tgaccatagc cgtctcctag atcatacgca ccatgatcat agttgtaagt
    39841 caactctaat tcatagtcat ccccaggaag tgtcaaataa acaagtgtaa atttgttttc
    39901 tggaaaatca cggcggcggc tttcttcaaa accaaatgcc ttttgataaa attcgattga
    39961 tgcttcaaga tctttcactc gaacacatgt gtgagccatt ttcatagatt catctctcct
    40021 tttcctttta ctcaatcata acaaaaagaa gtaaggaaat agttgatttg tgcttgttca
    40081 tgagaaattt ctcatgtaaa atggatagaa aataaaactg ttaaggtgga aactattcat
    40141 ggataaacca atttttggtg aaaaaaagca atttgctgat tatcaaaccc gttatgcagc
    40201 atatattgtc atagaaagag atcagcagat tgcagtaatc gaggctccaa atggtgcttt
    40261 ttttcttcct ggcggggaaa ttgaaggaga agagacgaaa gaagaagcga ttgtccgtga
    40321 gctgatagaa gaaatgggaa ttgtggcaga aatttcttct tatctgggag aagctgatga
    40381 gtatttttac tcgaattatc gtgcaactta ttattataat ccaggatatt tctttgttgc
    40441 tgattcttgg aagcgtatag gagaaccaac tgaaaagacc aataaaattt ggtgggtgag
    40501 ccctcaagaa gcgcttgtga aattgaaacg tggtagccac cgatgggcag tcgagagatg
    40561 gatcgaaaaa aataaaaact gaaagaaagc gagtagatca gctttctctc agttttttta
    40621 tttattacag tacatatttt ttgattgctt cagctacacc gtgctcgtcg tttgatgaag
    40681 tgatgacatt tgctgcttct ttgacaagag aaacggcatt tcccattgct actcctagac
    40741 cagcatattc aatcatggat aagtcgttct cgttatctcc gatagccatg atttcatctt
    40801 gatcaatacc aaggtgctgt gcgagattcg caactgccgc acctttactt gcttctttat
    40861 ttagtacttc gaagtaatag ggcgcacttt tgaccgttgt gtatttatca cggaaagttt
    40921 gcggtaaacg cgcaattgct gcatctaaaa tttctggttc gtcgatcatc atcattttga
    40981 cgatgttcat gtctggtgtc atttcttcag gtgttcgata ttttagcggc atcttgacta
    41041 aacttgcttc gtggatcgta taagcgctga tatctcgatt aggcgtgtag atcgctttgt
    41101 catcgatcga atggagatga ctgccgacac ggcgtgccat cacttcgatc tctaggaaat
    41161 cttcatgtgt cagtccgtga cgggcgatga ttttacctga tttcgtttgt tgtacgagtg
    41221 ccccgttata agtaataacg taatcattat cttgaaaaag gttcaactcg tttaactgtt
    41281 cctggacacc tggtaaggga cggccagtgc ataaaacgat cgaaacacct tttgcaacag
    41341 cagcggcaat cgcttctttt acttcttttg ttacttctct tttctcattt aataaagtac
    41401 catcgatgtc gatagcgact agtttgatag acataatttc ctccaatata ttttaattta
    41461 tttcaatcaa tgcgccatta cggatatggc tggcaaattc ttgataagtt tcgtcaaata
    41521 aatcataatg atcacgcaag gacggatcaa ccatttcttt tggaaagaaa aatcgctcat
    41581 ctccttgttc ttgtcccgct aatgcagcaa caagctgact aacctgtgat aattctatca
    41641 aagttccgtc atttcgcaaa atctcgattt gtgtgcgatg tctgccttgt tctgggcgat
    41701 aaaaatcata aggtagatcg tagctagaat tcacagcagt gtaataaaca ggattatatc
    41761 cgaccttttc tactaacaaa gtgagttctt ggactaattt tgaatcccgg ttgccagaaa
    41821 aagtagcaga tttcagtggt ttacgattca aaaaacgttt agctaaatca tttaaaatag
    41881 gatcagcaga ttcagaccat tgagtaaagt aagtgcccaa gaccccatca tcaagtttga
    41941 ggtattcttc taaagtaaag cttccgtcaa gaaatggcag aagtaattga gaatggagtt
    42001 caaaactgct ggcatcttct tgatacaact cgtgtgcgcg atgaagcaga tgctccaaaa
    42061 ttacttccat accacgggaa acaggatgga agtacacttg cacatacatt tgatatcggc
    42121 taacgatata atcttccact gcatgcatgc cactcatgga aaaagcgatc ccgccttcat
    42181 aaggacgaat cacacgtaat attcgggtaa gatcaaatgt tccatattct gttccagtga
    42241 aataagcatc ccgaagcaaa tagtccattc gatctgcatc gatctgacta gaaatcattt
    42301 ggacaacttg tgggttgggg taagtttttt ggatcacact agctaccttt tcaggaaaac
    42361 cttcttctac tcggttaaga atctgataca cttctgtttc tggagaagtg atgatctcta
    42421 cagtgatggc ttcatgattc gtatggaaga tatgttcaaa tgtatgagag taaggaccat
    42481 gaccgacatc atgcaacagt gccgcacata aagtcaccag ccgttcctga tcatcccagc
    42541 ctccgttgcc tattttttct tttgaaaaat ttcgagaaaa gatatcgcag attcgtcttg
    42601 aaatctcata aacgcctaat gaatgagtaa aacggctatg ttctgctccg tgaaaagtga
    42661 aagaagaagt gcctaattgt ttaatgcgtc gtagacgttg gacttctttc gaattgatca
    42721 aatctaagat tacttgatgc tggacatgga tatagttatg gactggatca cgaaaaactt
    42781 tttccatagg cagcatttga tatttataag gggttgtcat aatgtagctc ctttatgtta
    42841 ttacgttgtt ccattctctg gatttggttg ttccattgct ccagctggat ttcttttttt
    42901 agccagtcgg tagaatcctg taaatcactt gcagaataca gcttttcaaa agcttttgca
    42961 aatgcttctt tgacttcttt tacaaaaagg ggttggtcaa gcagttcttc aagtgttgcc
    43021 attgtagcag gatccactgc aggatagcca tttgtaccaa agtcttcttt taaagcagat
    43081 tgataaaatc gcttcatgac ttgcccgcgt ttttcctgat taccgttgac acttaagtac
    43141 atcataacag aaatcccatt tttgatccga cgctgagcga taccggcaaa ttttttgcca
    43201 tggatgctta aatcataagt tccaggacaa taagaatggg tgatttcgaa tgcttgtatc
    43261 ggtgcctttg gaaaagcgta tttagtcaat aggagcattt gctcgtaagc gtcatctatg
    43321 gttatttttt ttgtgtcact ttgggggaaa atcaaagaaa tattcaaaat tccttcatct
    43381 gatacgatcc ctaatccacc tgaatttctg atcactggag cgtaacctgc atctttcagt
    43441 gtttggatcc cgtcacttag aaaaggagtt cgaacatcct tcatacctaa gatgactgtg
    43501 cagtcatatt gccagaaatg taaaaagatt tgctggtgtt ctttagaata ttccgttaat
    43561 atatctgtta atgcaaaagg ggaaaagtcg tttttttgat aaatgtgctg atctagtcga
    43621 atcgcagggt tcattcaacc ggctcctttg tataaattca tattttctac aatattatac
    43681 tctaaaatca aagagttgtt ttggaaaaaa ggttttctgt aaaaatggac aagttggttg
    43741 acaaaacaaa agaaactagg aacaatgtag ctatgagaag taaaggagaa tgaataatgg
    43801 aattatcaca aggaacaccg atttcaatca aattgcgtac aaaagtaaaa cagcaagggg
    43861 aagtgcaaga tttttatttt gatttgaaag ggcaaatggt gacaattggc gatacgctgt
    43921 acatacgtta taaagagatt caggaggaga cgggcgatga gattcctgta acgatcaaat
    43981 tagttccaga tggtcatgta cagctgatcc gtgcaggaga aatgcggatg cgtttgaaat
    44041 ttggctataa agaacggtta gaaacatcct atcgaacacc atacggtatg atccagattg
    44101 ctacttttac gaaagaactg catgttagtt tgaaagaccg tcccacagga gggaaggttc
    44161 gaatcgatta tgatctgttc atgggaccgg aaaaaatcgg cgaatattat ttgaccttgg
    44221 attttacagc gtaaatataa aagaaaagtt tgattttttg tgtctgattt tgtatgataa
    44281 ggagtagact gtgaaaggac gtgtcctgat tggaaattag tgtatttgaa ggtgcaaaca
    44341 aaagcgaatt atcaatgatc gaagtagcac acgcgatctt agaacaacgt ggcgatgtca
    44401 tggatttttc tgatttagct aaccagatcc aaaactatct tgggaaatca gacagtgaaa
    44461 ttcgtgattc actcgcacaa ttttatactg acttgaatat tgacggcagc tttatctcat
    44521 taggggataa tcgttggggc ttgcgttcat ggtatgctat cgattcgatc gatgaagaag
    44581 taaaccatgg aatggacgaa gaggatgaag atactccacg tcgccgtaaa cgcaagaaag
    44641 tcaacgcctt tatcaatgat gatgaagacg cgatcgatta caatgacgat gacccagaag
    44701 atacagatct gacagaagaa gatgacgatg atctgtttga tgacgatgat gatgaagatg
    44761 aagaaatcgc tgcttataat tctgacttgc aagaaatcgg agcagatgac ccgacagatg
    44821 atgaagaaga cttgccagga aatatcgaag aagatttaag tatcatagac gaagacgatg
    44881 atgatgaaga ttatgaagag gaagaatttt ctgactctga aaaagaataa acattatttt
    44941 cttacgagag gttgtgacag agacgatcag cctatcagaa taaggaggtg tgacacgatt
    45001 tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa
    45061 attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat
    45121 gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg
    45181 actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa
    45241 agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa
    45301 agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt
    45361 agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc
    45421 ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga
    45481 cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat
    45541 tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa
    45601 tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa
    45661 atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg
    45721 agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga
    45781 gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc
    45841 tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg
    45901 aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga
    45961 agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact
    46021 ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg
    46081 atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga
    46141 ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa
    46201 tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc
    46261 tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat
    46321 ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg
    46381 ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc
    46441 aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga
    46501 caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact
    46561 agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag
    46621 agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt
    46681 tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc
    46741 ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac
    46801 tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc
    46861 atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc
    46921 aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa
    46981 agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga
    47041 aatcgtgaag cagacagctg aagcagggca cgagattggc aattacacat acgatcatca
    47101 gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga
    47161 gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac
    47221 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
    47281 ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
    47341 ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
    47401 cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc
    47461 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
    47521 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
    47581 aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg
    47641 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
    47701 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
    47761 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
    47821 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
    47881 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
    47941 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
    48001 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
    48061 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
    48121 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
    48181 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
    48241 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
    48301 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
    48361 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
    48421 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
    48481 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
    48541 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
    48601 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
    48661 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
    48721 tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
    48781 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
    48841 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
    48901 atttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
    48961 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
    49021 aagaagacaa aaacaagccg ttgtcggacc agaaattggt tgaattgctg aaaaaagatg
    49081 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt
    49141 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact
    49201 ttttctagtc tttttagttg aaaaacgaga gcgaaactgt tagacttaca gtcgtgaggt
    49261 tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg
    49321 ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat
    49381 gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat
    49441 aaaattttac gaaatatcta ttggatgcag ccgattgggc gtcgaagttt atcggaaagt
    49501 atggggatca cagaacgagt cttacgaaca gaaacagacc ttttgaaaaa tctgaatttg
    49561 atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc
    49621 cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa
    49681 tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg
    49741 gctgatgcat tcggcgaagc gctgagcgaa gccttagacc gactacttcc agaaggcgag
    49801 aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat
    49861 ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg
    49921 ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac
    49981 cgcgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag
    50041 ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt
    50101 gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg
    50161 aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc
    50221 gtttataagg tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc
    50281 atggcaatcg cgggcggaaa atcaaaagct aaagcaattc gagcttatat gaaaaatgca
    50341 ccaaaacaaa cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta
    50401 accctttaaa ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa
    50461 agtaggtatt aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt
    50521 agatggaatc gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt
    50581 gttaaaatac gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc
    50641 attcaacgta aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc
    50701 atggggcgaa ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc
    50761 agcagctgaa aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg
    50821 aaacgatgtt ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac
    50881 agttatctca ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa
    50941 cgacaaattt ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca
    51001 aatgactcta gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc
    51061 aaacatcgtt cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcccgaatt
    51121 gaacggtaaa ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga
    51181 attagtaaca gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga
    51241 agcttcaaac gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg
    51301 tatgacttac ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa
    51361 acaattagtt aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt
    51421 acgtacttta gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata
    51481 gacatgtaca tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag
    51541 tttattcagg aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca
    51601 aaaaagttct tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg
    51661 acactcgtat caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag
    51721 caatcctttt ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt
    51781 tagctccagt tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg
    51841 aaactcgcgg agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg
    51901 tattcgaaaa tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag
    51961 aactaggtaa atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag
    52021 ctcaccgtgc acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg
    52081 gattcttgat ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac
    52141 caatgattgc aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact
    52201 tgattcctaa agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag
    52261 caaaaggaat caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag
    52321 aattaatcga aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag
    52381 aattttcaaa tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg
    52441 ctttagacat cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa
    52501 cagttgtttg gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa
    52561 tcggtgtttg tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag
    52621 actcagccgc agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag
    52681 gtggcggcgc aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca
    52741 acgataaata aacgaccaag tttgctcaat tctgacgagt aatcgaacaa aaggtgaagg
    52801 gagttttcat gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt
    52861 aaacctatta tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt
    52921 gctgaagcag taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct
    52981 cctgcattat tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca
    53041 gcacaaaact gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct
    53101 cttgctgatc taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc
    53161 catgaaacag acgaagacat caataaaaaa gcgaaagcta tctttgcaaa tggtatgaca
    53221 ccaatcctat gctgcggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg
    53281 atcgaaggac aaattacagc tggtttagct gatctttcag ctgaacaggt gtcaaacatg
    53341 gttattgctt atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc
    53401 gctgacgaaa tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta
    53461 tcagaagcag ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac
    53521 atggcaaaag aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca
    53581 ttcttgtcat tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa
    53641 tgtttgcact aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa
    53701 aacaaactca aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat
    53761 cttagactca cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt
    53821 tggccgcgga atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg
    53881 tgacggagac aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa
    53941 caacatcatt gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa
    54001 agctatgatc gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt
    54061 aggtgtttct atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca
    54121 ctacctaggc ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg
    54181 cggatcacat gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc
    54241 tccaacattc aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat
    54301 cttgaaagct cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct
    54361 tggttcaaac gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt
    54421 acctggtaaa gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga
    54481 aaaaggtgtt tacgtactag ctgactcagg cgaaggcgaa aaaacaactg acgaaatgat
    54541 caaattctac gaagaattag tttctaaata tccaatcatc tcaatcgaag atggattaga
    54601 cgaaaacgac tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt
    54661 agttggtgac gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg
    54721 aattgctaac tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga
    54781 agctatcgaa atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga
    54841 aacagaagat tcaacaatct ctgatatcgc tgttgcaaca aacgctggcc aaatcaaaac
    54901 tggttctcta tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga
    54961 ccaacttggt gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa
    55021 ataattcttt tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac
    55081 ttagttaagt aatcgaactc tctacttgct gaaatatcag caggtagaga gtttttttgc
    55141 aactagcttc tgtatatgat gtttacaaag aatggtacaa gaaaagatca tccttgaaaa
    55201 aggaaagcta acaataacta gcatcatata agagctttta aggatagaac agtagaatag
    55261 gaatctatgg gcaagtcctc tcgtataaat aaaataggag ggaagtagca gcatattttt
    55321 cataaagtgg ctctctttct tcatttttac tttggttgct cctatttgaa aattcgagca
    55381 cactcaaata caataaaaag aaatggcaag ggagaggatt agatgaaaaa agaagaaaaa
    55441 atcgcgattt tacaagagat tatccgaatt aaatcagtga atggaaacga aggtgaagta
    55501 gcagcctatt tgaataagct tttggcaaag catgacataa caggagagat tgtttcttac
    55561 agagatggta gagataattt gattgctcgt taccaaaagg gccaatcagg aaaagtgtta
    55621 ggcctatctg gacatatgga tgtcgtggcg gctggtgatg aatcgtcttg gacctatgca
    55681 ccctttgcag cagaaatcca tgggaaccgt ttgtacggac gtggtgctac agatatgaag
    55741 agtggcttgg cagcaatggt gattgccatg atcgaactga aagagtcagg aaaac
//
LOCUS       DACDYJ010000010        54233 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDYJ010000010 DACDYJ010000000
VERSION     DACDYJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.010"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..722
                     /locus_tag="IWM51_000591"
     CDS             <1..722
                     /locus_tag="IWM51_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HAP9970030.1"
                     /translation="IITPGNIHDSQVAFQLVKQSKRLFPEINCVVADAGYKTPKFVHF
                     LTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYK
                     SNPKECVNCPLLNQCTKSKNHQRVITRHVWGDLMDEVEHLRLTDLNKSIYKKQKQTIE
                     RIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRN
                     EVDKKLFQARVTSLEQLLSTV"
     gene            complement(763..2142)
                     /locus_tag="IWM51_000592"
     CDS             complement(763..2142)
                     /locus_tag="IWM51_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HAP9970031.1"
                     /translation="MTKVTLKKVNPPKKDNEAKIYQLTDETMKFRKEKVLAAMEKSEI
                     DTLVIYCDLEHGGNFSYLTGFVTRFEESLLVLHQTGEAFLVLGNENTKMVDYSRIEAE
                     LIHTPLFSLPDQPMDNEKPLTEVLTNAKISKGKKVGLVGWKMFTSEEDNYQIQDLPAF
                     IVSSIVELVGEDAVSNRTDLFIHSDYGVRSLNTANEVAYYEFGSSLASDCVMNAIDEV
                     EVGKTEMEIGNHLVKYGQPNNVIPIAATGERFENAYIYPTDKQVKLGDKMSITTGFKG
                     GLASRSGYAVATTDDLPEGQKDYIEKVAAPYYDAVVAWLENIKIGMQGKELYSVIGKV
                     LPKSEYKWHLNPGHLTADEEWMSSPMKKDSEITLKSGMLLQIDIIPSVAGYAGASCES
                     GIALADESLRDQLAKEYPNVWSRIISRQEYIRNELGIELPDEVLPLSSGVGYYTPFFL
                     EKNLALVKG"
     gene            complement(2163..3710)
                     /locus_tag="IWM51_000593"
     CDS             complement(2163..3710)
                     /locus_tag="IWM51_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9970032.1"
                     /translation="MGPLSIIVASGLLLGIVSILQNPTLVGESFANATVIQGFVGGVQ
                     AIVGTMFGLLPILFAISVAIGMSREDKEIAAFSVVIAFILFHVVINYLLQLRGITAES
                     TSVETLMSDGMSQIKAVETSNMYETLLGTFTYRMGVFGGIIAGLWTAMIHNRYHTKQL
                     PAAFSFFSGNRFVPIMIILTIPIISIASFFIWPFVNTAINALGGLIGKSGVIGTFLYG
                     FSERLLIPTGLHHVLNQLIRFTPIGGTATINGETVSGALTIFNTELALPVKDMDILRS
                     ATRFLTQGTHPFMVFGLPAACYAMYKTSYLAQRPKIKGMLIAAAATCFVTGITEPIEF
                     AFIFISPILWVFHAFMAGLSFMLMNLFGVAVGNAGGGFIDLAIFGILQGTYTRWYLVV
                     ALGLVYAVAYYFVFKYVIERFDVKTPGRSNDDVLEDQDEEMTENELGKLILQGLGGAG
                     NIQEIDNCISRLRLVLKDTATIDEHILKKTGSMGIVKINENNLQVVYGGQVENATRSL
                     KNEMKRK"
     gene            complement(3959..4738)
                     /locus_tag="IWM51_000594"
     CDS             complement(3959..4738)
                     /locus_tag="IWM51_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9970033.1"
                     /translation="MTLEQLVNEHHSKLNENDDYVLSFITQNMEISISQTVSELAKQA
                     NVSDSTIIRLTKKLGFKGYGEFRYYLKEEFARMQKTRVDKSSLFLPEVLLGDVQATTK
                     LFEDDKSVEDIYETIRKADKIFAYATGYGQRMMLNEFARCMWNLGIYIIVVPEKNELD
                     LISKALTSNDLLFVISLSGRVDRIDDTLRRIHMKGTQLVSVTRFGQNNLASLSDYSLY
                     YQVTNINDVNELNNSSFCTLNLALSLLYEGYINYYKQSSVD"
     gene            complement(4813..5271)
                     /locus_tag="IWM51_000595"
     CDS             complement(4813..5271)
                     /locus_tag="IWM51_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9970034.1"
                     /translation="MGFFSRKEKLVAPVSGNLIPLSQVSDPVFSQGMMGEGFAVEPTN
                     AKVVAPIDGVIKSIFPTKHALTLTSNQGRDLLIHIGIDTVQLKGVGFQVLVEEGQRVS
                     SGESLVIFDCDQIKAEKLSDVVMVLFPEDKGIKLEVSEKKVSEGELLFKI"
     gene            complement(5283..6542)
                     /locus_tag="IWM51_000596"
     CDS             complement(5283..6542)
                     /locus_tag="IWM51_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HAP9970035.1"
                     /translation="MKTNFERIKKDIENLAEYNATPGEGVTRSAYSKEDREARNYLIN
                     QMEQLGLEIWEDGFSTLFGRRAGKKADAPSVMIGSHYDSVVNGGRFDGVAGVVTALET
                     MRILEENNFENDYPIDLILMNAEEGETFGPSTGVSNSRAMMGTLTMSELETVKNRHGQ
                     TKLEALRAYGVEPDLEAAKRDPKTIKNFIELHIEQGPVLEDEGKDIGLIEFIPGIGRF
                     KARFYGETEDSTAPMNKRKDALVAASKFVLFVEQWMKNLGEGIAGTVGKLTIKPNSNQ
                     FVPDFVEALIEIRTFDTKTTNPQEIKEKLLNELSVIQGETGVRTELEEMARINYSNPT
                     NPSVMDQGNVEKMKHICDQLGYSYQVINNGTGHDAMMMTDFVPTNMIYIPSKGGITHH
                     PDEWTDFEDVKKGADVLLNLVMDISKD"
     gene            complement(6658..6972)
                     /locus_tag="IWM51_000597"
     CDS             complement(6658..6972)
                     /locus_tag="IWM51_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9970036.1"
                     /translation="MDYEIQKFISEKEDQYFDRKSARIKPRDIIKHIVAFANADGGKL
                     VIGIEDDGTISGFKFQGAIDPWICVNKVDAFIESFLNQSTLELCYETRKSSYGGIYDN
                     DF"
     gene            complement(7118..8614)
                     /locus_tag="IWM51_000598"
     CDS             complement(7118..8614)
                     /locus_tag="IWM51_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAP9970037.1"
                     /translation="MNHQEAKQFFLKTSSFFSTSLPNYIDLSVGIEKAVDLLKNKPFT
                     ELVRHKRSLSEAVDVNHRILANKDGNYAWRPLQILHPLVYVDLVNGITQKNNWNHIKN
                     KFEEFQADKRIECISLPVESISSKSDTAETILNWWENLEQAQISYALEYDYCIHSDIT
                     DCYSSMYTHTIPWAVHSKEWAKTHRKDTQGVGNLIDSKIRYLQNGQTNGIPQGNVLMD
                     FVAEIVLGYADKMLLNKIEKTEIKEFKILRYRDDYRIFSVRKDQAEVIIRLLSEVLSD
                     LNLKLNSNKTFLSDNIILDAIKPDKIYWDLKHASFTEKSNNKWKFKLSLQKHLLQVKL
                     LGDKYPNCGSLSKALSEVYTHRVSTLNKRPDDIYQLISILVNIMQKNPRTLEACIVIL
                     GKLFEFIAVEEINLYLDKILRKFVNTPNTDIVEIWLQRLSLIHSREKSYSAKLCKKVS
                     NPKGFTLWNSDWLKKGFDESGIVNETFISNLSLTTPAKDLELFISQYE"
     gene            complement(8792..9271)
                     /locus_tag="IWM51_000599"
     CDS             complement(8792..9271)
                     /locus_tag="IWM51_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HAP9970038.1"
                     /translation="MKVYTVQPVDFLQSVDSEGFIYPKKRYTELSVFFDNPNAVGALH
                     WLEEEYTKRIQLPLDRDLIWLWRDTCHLKYARKDNFTDFSKYNFYCFEIPIEFYRKNI
                     LWSRFIEWHEPLNHEWETDENQEFEHIFDIPKHPGYGDIQGVTTRLHKDWIKKITHN"
     gene            complement(9647..9892)
                     /locus_tag="IWM51_000600"
     CDS             complement(9647..9892)
                     /locus_tag="IWM51_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9970039.1"
                     /translation="MLFLKEEKLIDKENVIGSNIRRIRLEKGMGQTELVRELQLSKIA
                     MTRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            9997..10770
                     /locus_tag="IWM51_000601"
     CDS             9997..10770
                     /locus_tag="IWM51_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970040.1"
                     /translation="MKKFAIILTCALLTGAISGCATSDTNSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSESNSIEKVLPIEIEKNQENIKLKAELPILNSTEIDW
                     HENIKVTIEKGALAQLYTEKVEQFFESGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLETPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHELPKPENEADMVIKIF"
     gene            complement(10999..11247)
                     /locus_tag="IWM51_000602"
     CDS             complement(10999..11247)
                     /locus_tag="IWM51_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9970041.1"
                     /translation="MDTITPTNFKKNIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMKNEKEDDFKVV"
     gene            complement(11346..11696)
                     /locus_tag="IWM51_000603"
     CDS             complement(11346..11696)
                     /locus_tag="IWM51_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970042.1"
                     /translation="MIIENESEEIGRMADENKVVPLETLVINKEEFEQLFLDFFVQYD
                     RIYDFSSSLDLFELEAEIIKNSQEEDGNIIISVEKFLELFSEIFEIDIDYYQPQEVFR
                     LFRDQIIGISQTTK"
     gene            complement(11737..12231)
                     /locus_tag="IWM51_000604"
     CDS             complement(11737..12231)
                     /locus_tag="IWM51_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970043.1"
                     /translation="MFKVYFPFQWLFKGILLLLLGLILFGSYLTIKARYFPEEKPVKI
                     VTKDLKMGETYQADGVEITFKKVELTGKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENKNGEELYEYAMILDNPEYIESGKSETINYYYELPSERQEYQIVVRRANGYFVGL
                     PFNL"
     gene            complement(12224..12526)
                     /locus_tag="IWM51_000605"
     CDS             complement(12224..12526)
                     /locus_tag="IWM51_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970044.1"
                     /translation="MSDPYIKVYCSKETKTIFDSFYEQNKGIFGTKANMFRVMVSNLP
                     VLANQSNVKLSNPESKGIEQKISEIESIVVGKLDEIDQKLSYYLKNKCKTEEGKDV"
     gene            complement(12742..14133)
                     /locus_tag="IWM51_000606"
     CDS             complement(12742..14133)
                     /locus_tag="IWM51_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9970045.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDNSEE
                     ALQEMNLVRTRMNKSEDIAQAFHVIHSFDKNISKELSIEKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNIDMETCKRLRIDPKDLERMRKINDEVLVENGLEPVDNQY
                     YENLEKRTNLYSNDPSKLKNGKINYQEFVQNAIEATLENCEVNSFVRFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQDVNFYERRDELITLGREKQSRKEKSQYVERTFSARKLGK
                     KYSFESVFERLEKNLEETHMNNFVDLDTEQIIALASGVDYKTGELNVPFVELDSIAQK
                     QFSDHEGAKKIIKYDDFLREVDRHYANTISLEDDQEYTIKLHTDVNPRTLRSTKSKTI
                     LVKKTDQKELFLPAEGFKNCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQRRQRDFEMDF"
     gene            complement(14118..14729)
                     /locus_tag="IWM51_000607"
     CDS             complement(14118..14729)
                     /locus_tag="IWM51_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAP9970046.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVALIKQLNQIGNNLNQAVKALNIIAKYFREQSENKKEK
                     SLHFSFDVSRYNIFKDELANSIAKKMMISYPDDIFLESNRSTSSFLKLSEVEKDYFQY
                     WLEKQTRPDTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            complement(14741..14929)
                     /locus_tag="IWM51_000608"
     CDS             complement(14741..14929)
                     /locus_tag="IWM51_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970047.1"
                     /translation="MQLILQISTQDFLELAELCDSVKKATNEKITVTNYIELMIQEEL
                     MMKNEIESNFKCCSLEKM"
     gene            complement(14943..17480)
                     /locus_tag="IWM51_000609"
     CDS             complement(14943..17480)
                     /locus_tag="IWM51_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970048.1"
                     /translation="MKIIKKKIANRKTNWLPVIFLVLIAVILFTSNIAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     RFVESTGLNKVYQAVLSTIVVGLMILSLVIIGYKMITGKGTVDLKSVGMNVVMSISLI
                     LLMPTLIHSGIEFSKTFYNDTTTITNSDEGVAWSLIEQGVTDLAYINKTDQYSMIDKT
                     NDRNNLTKENFWKTDLTQVLTDNVLDKMEKENPIADNLRYELVENSDNEFVATKFSDN
                     FLSTFVEGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLGTGSGSSVNPFIKAVA
                     YVCAVIVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTVYAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNINPASAIQQKVKQDIEERKNAMHGQAKS
                     AEVLINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKSVKENLQNSTSSQDRRSVDVRENLQNTTSSQNGNRTVYVRENVQSSGTSQPRTVDV
                     RENLQNTTSSQNGNRTVDVRENVQSSASNQPRTVEVRETRAEASQGDKTQKINIIEER
                     KERPNEKVIVEQEVKEKKRPRFTYENNPLFQDISDKPNPLFDSLFKK"
     gene            complement(17467..17685)
                     /locus_tag="IWM51_000610"
     CDS             complement(17467..17685)
                     /locus_tag="IWM51_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970049.1"
                     /translation="MIENHLVDKIHCLDHNADTLELTESICSNTIVLDLKAVNDDYVT
                     HIIIDRKIAQKLSESLRKWAEGGNNENN"
     gene            complement(17687..18730)
                     /locus_tag="IWM51_000611"
     CDS             complement(17687..18730)
                     /locus_tag="IWM51_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP9970050.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKNKMQMFPNFYYLLKPVLCKDDVYKINKITHQENSQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLFESLS
                     TKNNKLDEELIETFIEENLLKQMWGESIVNCLKLASNSDYRQFDNWYKKFNYAIKSAE
                     KEQKVQLKIIYEICNNSYFVDHVREQLAMTLRDLIRRAKTDHRIKQKNNYIFTSLKNK
                     ALELIKLQKKEGI"
     gene            complement(18885..19211)
                     /locus_tag="IWM51_000612"
     CDS             complement(18885..19211)
                     /locus_tag="IWM51_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970051.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(19219..19422)
                     /locus_tag="IWM51_000613"
     CDS             complement(19219..19422)
                     /locus_tag="IWM51_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9970052.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKSK"
     gene            19497..20147
                     /locus_tag="IWM51_000614"
     CDS             19497..20147
                     /locus_tag="IWM51_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9970053.1"
                     /translation="MSIKTLLNLWFLCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            20202..21368
                     /locus_tag="IWM51_000615"
     CDS             20202..21368
                     /locus_tag="IWM51_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9970054.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(21457..21849)
                     /gene="rpsI"
                     /locus_tag="IWM51_000616"
     CDS             complement(21457..21849)
                     /gene="rpsI"
                     /locus_tag="IWM51_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9970055.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(21865..22308)
                     /gene="rplM"
                     /locus_tag="IWM51_000617"
     CDS             complement(21865..22308)
                     /gene="rplM"
                     /locus_tag="IWM51_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP9970056.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(22827..23294)
                     /locus_tag="IWM51_000618"
     CDS             complement(22827..23294)
                     /locus_tag="IWM51_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP9970057.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(23414..24952)
                     /locus_tag="IWM51_000619"
     CDS             complement(23414..24952)
                     /locus_tag="IWM51_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP9970058.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            25032..25718
                     /locus_tag="IWM51_000620"
     CDS             25032..25718
                     /locus_tag="IWM51_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP9970059.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(25919..26446)
                     /gene="msrA"
                     /locus_tag="IWM51_000621"
     CDS             complement(25919..26446)
                     /gene="msrA"
                     /locus_tag="IWM51_000621"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9970060.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(26530..27207)
                     /gene="pgmB"
                     /locus_tag="IWM51_000622"
     CDS             complement(26530..27207)
                     /gene="pgmB"
                     /locus_tag="IWM51_000622"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9970061.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(27204..28334)
                     /locus_tag="IWM51_000623"
     CDS             complement(27204..28334)
                     /locus_tag="IWM51_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9970062.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(28334..30517)
                     /locus_tag="IWM51_000624"
     CDS             complement(28334..30517)
                     /locus_tag="IWM51_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9970063.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(30514..31539)
                     /locus_tag="IWM51_000625"
     CDS             complement(30514..31539)
                     /locus_tag="IWM51_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9970064.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(31584..32411)
                     /locus_tag="IWM51_000626"
     CDS             complement(31584..32411)
                     /locus_tag="IWM51_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9970065.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(32408..33475)
                     /locus_tag="IWM51_000627"
     CDS             complement(32408..33475)
                     /locus_tag="IWM51_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP9970066.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(33501..34337)
                     /locus_tag="IWM51_000628"
     CDS             complement(33501..34337)
                     /locus_tag="IWM51_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9970067.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(34334..35203)
                     /locus_tag="IWM51_000629"
     CDS             complement(34334..35203)
                     /locus_tag="IWM51_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9970068.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(35273..36556)
                     /locus_tag="IWM51_000630"
     CDS             complement(35273..36556)
                     /locus_tag="IWM51_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9970069.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(36570..38255)
                     /locus_tag="IWM51_000631"
     CDS             complement(36570..38255)
                     /locus_tag="IWM51_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP9970070.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            38530..39555
                     /locus_tag="IWM51_000632"
     CDS             38530..39555
                     /locus_tag="IWM51_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9970071.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(40150..43803)
                     /gene="rpoC"
                     /locus_tag="IWM51_000633"
     CDS             complement(40150..43803)
                     /gene="rpoC"
                     /locus_tag="IWM51_000633"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP9970072.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(43882..47499)
                     /gene="rpoB"
                     /locus_tag="IWM51_000634"
     CDS             complement(43882..47499)
                     /gene="rpoB"
                     /locus_tag="IWM51_000634"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9970073.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            48227..49195
                     /locus_tag="IWM51_000635"
     CDS             48227..49195
                     /locus_tag="IWM51_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9970074.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            49213..50118
                     /locus_tag="IWM51_000636"
     CDS             49213..50118
                     /locus_tag="IWM51_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9970075.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            50115..50921
                     /locus_tag="IWM51_000637"
     CDS             50115..50921
                     /locus_tag="IWM51_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9970076.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            51078..52046
                     /locus_tag="IWM51_000638"
     CDS             51078..52046
                     /locus_tag="IWM51_000638"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP9970077.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(52363..52887)
                     /locus_tag="IWM51_000639"
     CDS             complement(52363..52887)
                     /locus_tag="IWM51_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP9970078.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            53174..>54233
                     /gene="scm"
                     /locus_tag="IWM51_000640"
     CDS             53174..>54233
                     /gene="scm"
                     /locus_tag="IWM51_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9970079.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSD"
BASE COUNT        15796 a        11297 c         9339 g        17801 t
ORIGIN      
        1 caatcatcac tcctggaaat attcatgaca gtcaagtcgc ctttcagctc gtcaaacaat
       61 cgaagagact ttttcctgaa atcaattgtg ttgtcgctga tgctgggtat aagacaccta
      121 agtttgttca ctttttgact catttgaacc ttcgaccttg tttgccttat tcaaggccca
      181 aagggaaaaa ggggctatta tcaaaaaatg agtttctcta tgacgagtat tttgattgtt
      241 atatttgtcc gcaagatcaa atgctcgctt tttcaacggt tactcgagaa gggtaccgag
      301 aatacaaatc caatccaaaa gaatgtgtga actgtcctct gctaaaccaa tgtaccaaaa
      361 gtaaaaatca tcaacgagtc atcacgagac atgtttgggg agatctgatg gatgaagtgg
      421 aacatcttcg cctaacagat ctgaataaat caatttacaa aaaacagaaa caaacgattg
      481 aacgaatatt tgcggatgca aaagaaaagc atgggatgcg ctggaccaag tatcgaggct
      541 tggaaaaagt cgcgacgcat acgatgctag tgtttgctgc aatgaattta aagaaattag
      601 ccacctggct atggaaaggc aaggagcctt tatttttttg ttcaaaaatt cgaaacgaag
      661 tagataaaaa gctgttccaa gctcgagtta cgagtttgga acagcttttg tctacagtct
      721 gaatcatttc ttccgtttta aagaagaaat gattttcata gatcaacctt taactaaagc
      781 tagattcttt tctaaaaaga atggtgtgta gtagcccaca ccgctggaca acggtaaaac
      841 ttcatccggc aactcgatgc ccagttcgtt acggatgtac tcttggcgag aaatgattcg
      901 cgaccaaaca tttggatatt ctttggctag ttgatcacga agcgactcat cagctagcgc
      961 aatcccactc tcacagcttg cgccggcata gccagcaact gatggaataa tatcaatttg
     1021 tagtaacatt ccggacttaa gagtaatttc tgagtctttt ttcattggcg aagacatcca
     1081 ctcttcatca gccgtaagat gccccggatt taagtgccat ttgtattccg atttaggcaa
     1141 gactttacct attactgaat atagctcttt accttgcatt cctattttga tattttccaa
     1201 ccatgcgacc acggcgtcgt agtatggagc tgcaactttt tcaatgtaat ccttttgccc
     1261 ttctggtagg tcatctgtcg ttgcaactgc atagccactt cgacttgcta atccgccttt
     1321 aaagcccgta gtaattgaca ttttatcgcc tagtttaact tgtttatcag ttggatatat
     1381 ataggcgttc tcaaaccgtt ccccagttgc agcaattgga atgacattat ttggttggcc
     1441 gtacttaact aagtggttac caatttccat ttctgttttt cctacctcta cttcatcaat
     1501 agcgttcatc acacaatctg aagccaagga tgagccaaat tcataatatg ccacttcatt
     1561 agcagtattt aatgagcgta ctccgtaatc actgtgaatg aacaggtcgg tgcgattgct
     1621 aacggcatct tcccctacta attctacaat cgaacttaca atgaatgccg gcaaatcttg
     1681 gatttggtag ttatcctctt ctgaagtaaa catcttccaa ccaacaagcc caactttctt
     1741 acctttactg attttagcat tggtcaatac ttcagttagt ggtttctcgt tatccatcgg
     1801 ttgatccggt aatgagaaga gtggcgtgtg gattaattct gcctcaattc ttgaatagtc
     1861 caccatttta gtattttcat ttcctagaac taagaaagct tcacccgtct gatgtaatac
     1921 caacaagctt tcttcaaagc gagtaacaaa acctgttaaa taagagaagt tcccgccatg
     1981 ttccaaatca caatagatta cgagggtatc tatctctgac ttttccatcg ctgcaagtac
     2041 cttttcttta cggaacttca ttgtttcatc agtcaattga tatattttag cttcattatc
     2101 ttttttaggt gggttaactt tttttagagt taccttagtc atattgtcac tactttctgt
     2161 ttttatttac gcttcatttc atttttcaga gatcgagttg cgttttccac ttgtcctcca
     2221 tataccactt gtaaattatt ttcgttaatt ttaacgattc ccattgagcc ggtctttttc
     2281 aagatatgtt catcaattgt tgctgtatct ttaagaacta gtctcagccg agagatacag
     2341 ttatcgattt cttgaatatt accagcacca ccaagtcctt gcaagattag tttgccaagt
     2401 tcgttttctg tcatttcttc gtcctgatct tccaacacat catcgttgga tcgaccagga
     2461 gttttcacat caaatcgctc aatgacatac ttaaatacaa agtaatatgc aactgcataa
     2521 acaaggccca atgctacaac taagtaccaa cgagtataag taccttgtaa aattccgaag
     2581 atagccaaat caataaatcc accaccggca ttcccaactg cgactccgaa tagattcatt
     2641 aacataaagg ataaaccagc catgaatgca tggaagaccc atagaatcgg tgagataaag
     2701 ataaaggcga attcaattgg ctcagtaatc ccggttacaa aacaagtagc cgctgctgca
     2761 atcaacatcc ctttaatctt tggacgttgc gccaaatatg aagttttgta cattgcatag
     2821 catgctgccg gcaatccgaa gaccataaat ggatgagtac cttgtgtcaa gaagcgagta
     2881 gcacttctca aaatatccat gtcttttact ggcaatgcta attcagtatt gaagatggtt
     2941 aatgctccag aaactgtttc accattgata gttgccgtac cgccgattgg tgtaaatcga
     3001 atcaactggt ttagtacatg gtgaagacct gttggaatca gcaggcgctc tgagaagcca
     3061 tataaaaaag ttccaattac gcctgactta ccaatcaaac cgcctaaagc attgatagct
     3121 gtattgacaa aaggccagat gaaaaatgaa gcgattgaga tgattggaat agttaggatg
     3181 atcataattg gaacaaaacg attgccacta aagaaactga aagctgctgg taattgtttt
     3241 gtgtgatagc gattgtggat catggctgtc cataatcctg caatgatacc gccgaaaaca
     3301 cccatacgat atgtgaatgt gccaagtaaa gtttcataca tattggaagt ttctactgct
     3361 ttgatttgac tcattccgtc agacatcaat gtttcaacag aagtgctttc cgctgtgatg
     3421 cctctaagct gaagcagata attgataact acatggaaca gaataaatgc aataaccact
     3481 gaaaatgctg caatttcctt atcttctcta gacatcccga ttgctactga aatagcaaat
     3541 agaataggaa gtagtccaaa catcgtacca acaatagcct gtactccacc aacaaatcct
     3601 tggatcacgg ttgcattagc gaagctttct cccaccaaag taggattttg taaaattgaa
     3661 acgattccta gtagtagtcc cgatgcgaca ataatagaca acggacccat taatgatttc
     3721 cctaacttct ggatataagt tctcaatata aacaccttct tctttttagt tgatataaac
     3781 gtttacatcc cttgtacaac gaaagtatag caccctgaaa aagcgctaac aatacgatag
     3841 gtctgataaa aatcaacatt cagcacttgg ttgttcattc aaaacgtgaa tcttcaatca
     3901 cagaaaaata cgcgctatca agcacgtatt ttaggtgtaa atatatgcat caactttatt
     3961 aatcaacgct actttgtttg taatagttaa tatacccttc atacagtaag ctaagagcaa
     4021 gattcaaggt gcagaatgat gagttgttca actcattcac atcattaatg ttggtcactt
     4081 gatagtatag gctatagtct gacagactgg ctaagttatt ttgaccaaat cgtgtaactg
     4141 agaccaactg tgtccctttc atatggattc ttcttaaagt atcatcaatc cgatcaacac
     4201 gtccggacaa tgatatgaca aatagtaaat cattcgaggt caaagctttt gaaatcaggt
     4261 ctaattcatt cttttccggt acaacgataa tgtagattcc caagttccac atgcagcgtg
     4321 caaactcatt gagcatcatt ctttgtccat atcctgttgc gtaggcaaat atcttatctg
     4381 cctttctaat cgtttcatat atatcctcaa cacttttatc atcttcaaac aatttggttg
     4441 tcgcttgaac atctccgagt aatacttccg gtaaaaacaa actactttta tcgactctgg
     4501 ttttttgcat gcgtgcaaat tcctctttaa ggtaatatcg aaattcgcca tatcctttga
     4561 atccgagttt cttagttagt ctaataatcg ttgagtcaga tacatttgct tgttttgcta
     4621 attccgatac agtttgactg atgctgattt ccatattttg agtgataaaa cttaatacat
     4681 aatcatcatt ctcattcaat ttactgtgat gttcatttac taattgctcc aatgtcataa
     4741 tcagacctcc aaaaataata aaaaagattg ctctctcatt ttaatacgaa agcacaatct
     4801 ttttaagtac tattaaattt tgaaaagaag ttctccttca gaaacctttt tttcagagac
     4861 ttctagttta atacccttat cttcgggaaa tagaaccata acaacatctg acagtttttc
     4921 cgccttgatt tggtcacaat caaaaatcac caatgactcg ccggatgata ctcgttgacc
     4981 ttcttcaacg agcacttgaa acccgactcc ctttaactga acagtatcaa ttccaatatg
     5041 aatcaataaa tctctacctt gattactagt caatgtgagt gcatgctttg ttggaaatat
     5101 cgatttaatg acaccgtcaa tcggtgctac caccttagcg tttgttggtt caacagcgaa
     5161 tccttcgccc atcatccctt gcgaaaatac aggatcgctt acttggctaa ggggaatcag
     5221 atttcctgat actggagcaa ctaatttttc ttttcgacta aaaaatccca ttgacttact
     5281 ccttaatctt ttgaaatgtc catcacaagg tttaaaagaa catccgcccc ttttttgaca
     5341 tcctcaaaat cagtccactc atcaggatga tgtgtaatgc cacctttgga tggaatataa
     5401 atcatatttg ttgggacaaa gtcggtcatc atcatcgcat catgtccagt accattgtta
     5461 atgacttgat agctatagcc caactgatcg caaatatgtt tcatcttctc aacattacct
     5521 tgatccataa cacttggatt tgtaggatta ctgtagttaa ttcgagccat ttcctccagc
     5581 tcagttctaa ctccagtttc cccttgaatc actgataact cattcaacaa cttttcttta
     5641 atctcttgag gattagttgt ctttgtatca aaggttctga tttcgattaa agcttcgaca
     5701 aagtcaggga caaattgatt tgagttcggt ttgattgtaa gtttaccgac ggttccggca
     5761 atcccttcac caagattctt catccactgt tcaacaaaaa gaacaaattt agaagcagca
     5821 actaaagcat ctttccgttt attcattgga gcagtcgagt cctcagtttc tccataaaaa
     5881 cgtgctttaa aacggccgat gccaggaata aattcaatca atccgatatc tttgccttca
     5941 tcttccagga ctggtccttg ttcgatatga agttcaataa aatttttgat tgtttttgga
     6001 tctcgcttag cggcctctaa atctggttcc actccataag cacgaagagc ttccaatttt
     6061 gtttgtccat ggcgattttt tactgtttcc aactcactca tggttaatgt tcccatcata
     6121 gctcgtgaat ttgagacacc cgtacttggc ccaaatgtct cgccctcctc tgcattcatc
     6181 agaattaggt caattgggta atcattttca aaattatttt cttccagaat ccgcattgtt
     6241 tccagtgcag taacaacgcc agccactccg tcaaatctgc ctccattaac aactgaatca
     6301 taatgagagc caatcatcac tgatggtgca tcagcctttt taccggctct acgaccaaat
     6361 aatgtagaga agccgtcttc ccaaatctct aaacccagtt gttccatttg attgatcaaa
     6421 taatttcttg cttcacgatc ttctttagag taagcagagc gggtcactcc ttcacctgga
     6481 gttgcattgt actcggctaa attttcgata tcctttttta tacgctcaaa atttgtcttc
     6541 ataattaaat cctccatttc ctacaagtga atgttagcac ccatagaaag cggctacaat
     6601 ataatcaatg aagattgaat cgatattcga aaactgaata ttgcttcggt taatgactta
     6661 gaaatcatta tcgtagattc caccataaga actttttctc gtctcataac acaactcgag
     6721 agtggattgg tttaaaaaac tctcgataaa tgcgtccact ttattgacac agatccatgg
     6781 atcgatcgca ccttgaaatt taaacccaga aatagtccca tcatcttcta taccgattac
     6841 cagcttacct ccatcggcat ttgcaaacgc tactatgtgt tttataatat ctctaggctt
     6901 aatccgtgca ctttttctat caaaatactg atcttctttt tcacttataa acttttgaat
     6961 ctcataatcc atttaaaatt tcccttctta aattttttta ttatagagcc gtatattcaa
     7021 tttccacaaa aaaataatct aaaacaaaac agacaccttc attttgatat gttctcccac
     7081 atatgaatcg gtcgctatac agcattacat atagtaatta ttcatactgc gaaataaata
     7141 gctccaaatc tttagctggc gtagttaagc ttaaattcga aatgaaagtt tcattgacta
     7201 ttccactttc atcaaatccc ttcttcaacc aatcagagtt ccacaacgta aatcctttag
     7261 gattagaaac tttcttacat aattttgcac tataggattt ctctcttgaa tgaattaaag
     7321 aaagtctttg taaccaaatt tcaactatat ccgtattagg agtattaaca aattttctta
     7381 agattttatc caagtataag ttgatttctt ctacagcaat aaactcaaaa agcttaccta
     7441 aaatcacaat acaagcctcc aaagttctag gattcttttg cattatatta acaagaatac
     7501 taatcaattg gtagatatca tctggtcttt tgtttaaagt agaaactcta tgtgtataga
     7561 cttcacttag cgctttactt aaactcccgc aatttggata tttgtctcct aaaagcttta
     7621 cctgtagcaa atgcttttga aggctaagct taaacttcca cttattatta cttttctctg
     7681 taaaacttgc atgtttaaga tcccagtaaa ttttatcagg cttaattgca tctaaaatta
     7741 tattatccga taagaatgtt ttatttgaat ttagcttcaa atttagatcc gataatactt
     7801 cagataacaa tctgataatc acttcagctt gatcttttcg aaccgaaaaa attctgtaat
     7861 catctctgta acgaagtatt ttgaactctt taatctctgt tttttctatc ttgttcagta
     7921 acattttatc agcataccca agtactattt cagctacaaa atccatcaat acatttcctt
     7981 gaggtatacc gttcgtttgt ccattttgta aatatcgtat tttagaatct attaagtttc
     8041 caacgccttg agtatctttt ctatgtgtct tagcccattc ttttgaatgt actgcccacg
     8101 ggatagtatg tgtgtacatt gagctataac aatcggtaat atctgaatga atgcagtagt
     8161 catactccaa agcataacta atctgtgctt gttcaaggtt ctcccaccaa tttaaaatag
     8221 tttccgcagt atcgctcttt gaagaaatag attccacagg aagtgatata cattcaattc
     8281 gcttgtcggc ttggaattct tcaaacttat ttttgatgtg attccaatta tttttctgtg
     8341 taatcccgtt tactaagtct acatatacta atggatgtaa tatttgaagg ggtctccaag
     8401 cataattacc atctttatta gctaatattc gatgattaac atcaactgcc tcagagagcg
     8461 atctcttatg tctaactagt tcagtaaatg gtttgttttt taataaatcc actgctttct
     8521 caatgcctac tgataaatca atatagttgg gcaacgatgt actaaaaaaa cttgatgttt
     8581 ttaaaaagaa ttgctttgct tcttgatgat tcatctctaa aatacttttt ctagtcctag
     8641 tcaaattcaa aatctcctag atcaattaag ttgtaatgta agaggatacc ctaaggttac
     8701 acaccctatt tactattata ccagtattca ttcgtgatac ataagggtgt aacattaaga
     8761 actctatcta aaagactaca agtagtcacc tttaattgtg agtaattttc tttatccaat
     8821 ccttgtgcaa ccttgtcgtc accccttgta tatccccgta ccccggatgt ttcggaatat
     8881 caaaaatgtg ctcaaattcc tgattttcat cagtctccca ctcatgattc aacggttcat
     8941 gccactctat aaaccttgac cataaaatgt tctttctata aaactcaata ggaatttcaa
     9001 aacagtaaaa attgtatttg ctaaaatctg taaaattatc tttacgagca tactttaaat
     9061 ggcaggtatc tcgccacaac caaattaaat ctcgatccaa tggtaactga attcgtttcg
     9121 tatactcttc ttcaagccaa tgaagtgcac caactgcatt cggattatcg aaaaagacag
     9181 ataattcagt atagcgtttc tttggataaa taaatccttc tgaatcaaca gattgcaaaa
     9241 aatctactgg ttgaaccgtg tacactttca tttattcgcc accttttttt gactacttat
     9301 agtcatttac caatttgaaa tctgcatact ttttcattca attgagccca gcattaattg
     9361 tacacgagtt ttcttcacta acggatagta tcaggatata ctaatactat cctgaatagc
     9421 gaaatgaggt agccaaaatg agaaaagtcg atgaatatat caaaaagaaa tcccaaaaaa
     9481 gtaaagtatg gcaaagaaca tttgagttag agtataagaa actctcagat agcctcaaaa
     9541 aggacactta taatccaaat gaacaaagta aagcagataa ccaataaaga ttaccctccc
     9601 atatttaaca ttgcttcacg aatcagtaat caatgactac aagtagtcat tgaagcaaat
     9661 cttcataaga tacctgtaga atatccctaa tagctctcaa ttgatccagc ttaatatgtt
     9721 gccggccacc ttcaatctta accaatgttt ctcttgtcat agcgatttta ctgagctgga
     9781 gttctcgcac cagttctgtc tgtcccattc ccttttctaa tcggattcgt ctgatattgc
     9841 taccgattac attttcttta tctattaact tttcttcctt caaaaatagc acgccttttg
     9901 gactaaaatt agtccttttt cttattaatc ttaaagattt ggtatgctac aatgggacta
     9961 gtattagtcc atattcaatt ttaaggagga atttttttga agaaatttgc aatcattcta
    10021 acctgtgctt tactcacggg agctataagt gggtgcgcca cttctgacac aaattctaca
    10081 agtactgaaa caagtacaac aacctcagaa aagaattatc ctatacattt ttttccagtt
    10141 ccagatggat ttgaagctta ttatcatgat aaaaaactag atgttccatt gtcagaaagt
    10201 aactctattg aaaaagtttt gccaattgaa attgaaaaaa atcaagagaa tataaaactc
    10261 aaggccgagc ttcccatact gaacagcaca gaaattgatt ggcacgagaa tattaaagta
    10321 actattgaaa aaggagcgtt agcacaactt tatactgaaa aagttgaaca attttttgag
    10381 tctggtgcca ataaagactt ttcaaaaata agcgattatt cggataactt ttttaatgag
    10441 acccaagatg aaatcacgaa ctcaactttt atggctgaag atatgtacaa tatgacgtta
    10501 aaaaaaataa aagcttacga ccatccatta aatctgacac taaataatgg attactctct
    10561 tttgtattag caggttacgt tgactatgaa tattcaccag attatttgga gacacctctt
    10621 caacacgaga acaatgatat taattttaga tttgtctacg agtcaaaaag taaaacatgg
    10681 ttagttgatc aattaagcac tataaacaca agcttccatg aattacctaa acctgaaaat
    10741 gaagctgata tggtcattaa aatcttctaa aaaagaagcg ttgaaggaga aatattcctt
    10801 tcaacgctct tttttatatg cttagaaaca taaataaatt gtatactttc tttttagccc
    10861 ctattacttt tcacgaaaaa tatctttttt cgacactagc gtttctctac ttataatatt
    10921 gtacacagtt tttgtattat tgaaaaatta ctcattattt agcattaatc cacatcgttt
    10981 tatcaatcga ccttcatact aaactacttt aaagtcatct tccttctcat ttttcattaa
    11041 atcagataca taagcccatg tacccgttcg ttgtaaataa agttcttctt gtagcagctc
    11101 ccactttctc ttagaaatca taatgacgcc atcgttcaat ccatttttag aatccctagt
    11161 aatttctatt tcagaatttg aatgaactac ttcttgcaac actttaccta tatttttttt
    11221 gaagtttgtt ggagtaatag tatccaaaat gaacacctct cttctctata cctctattat
    11281 gaagcgccca tcaaataaag caagcgttga ggaataattc tttcaacgct tgctttatta
    11341 agctttcatt ttgtagtttg actaatacca ataatctgat ctctaaacaa tcgaaaaact
    11401 tcttgtggtt gataatagtc tatatctatt tcaaatattt ccgaaaacaa ttcaaggaac
    11461 ttttctactg aaatgataat gttaccatct tcctcttggc tgttttttat tatttctgct
    11521 tctaattcaa ataaatctag ggacgaacta aaatcataaa tcctgtcata ttgaacaaaa
    11581 aaatcaagaa aaagttgctc aaactcttcc ttatttatca ctagtgtttc aagcggaaca
    11641 actttatttt catcagccat tcttccaatt tcctcacttt cattttcaat aatcacaaaa
    11701 atgacattaa cttaaaaacc actaacaaaa taagacttac aaattaaagg gtaatccaac
    11761 aaaataacca ttcgctctac gtactacgat ttggtattcc tgcctttcac taggtaattc
    11821 ataatagtaa tttattgttt ctgatttacc actttcaata tactctggat tatccaatat
    11881 catagcatac tcataaagtt cttcaccatt cttattttca atcgaccatt gttgcggacg
    11941 tattggattt tctgtatcat ttttcattgt ataggtcact tgtaatacac gatcatgttg
    12001 cgcaaattca tttctttttc cggtcaattc aaccttttta aacgttattt cgaccccatc
    12061 tgcctgataa gtttcaccca tttttaaatc tttagttaca atttttactg gtttttcttc
    12121 tggaaaatat ctggctttaa ttgttagata gcttccaaaa agaatcaatc ctagtagcaa
    12181 taaaagaatt cctttaaaga gccactgaaa cggaaaatac actttaaaca tctttcccct
    12241 cctccgtttt gcatttgttt tttagataat acgataactt ttgatcaatt tcatccaact
    12301 ttcccaccac aattgattct atttcagaaa ttttttgttc aattcctttg ctttcaggat
    12361 tacttaattt tacatttgat tggttagcta acactggtaa atttgatacc atcacacgaa
    12421 acatattggc ttttgttccg aaaatccctt tgttctgttc gtaaaaactg tcaaaaattg
    12481 ttttagtttc ttttgaacaa tacactttaa tatacggatc agacatgtgt ttttactcct
    12541 tatctttttt tatttaggga gtctgtaggg agcttgctcc ctcagaggtt ttccccatct
    12601 aacttgccag aaagtagccc tgcgtaagca agggcggatt gactggcaag tagatgggta
    12661 gctggcgtat gccctcaaaa ccgcctcttt ccaattccct ccgtatcgat gggaaactgg
    12721 aaagtctact ggctaattcc tttagaaatc catctcaaaa tctcgttgtc tcctttgctg
    12781 attttcaatc atttgttgcg tgaactcgta atctttttta gcacgataca ttttcacatc
    12841 gtcattcatt atgcgactga tttgatagtg aatctgttta gcagaaggtg tatgagaaca
    12901 atttttaaag ccctcagctg gtagaaataa ttctttctga tctgtctttt taactaaaat
    12961 tgttttactt ttagtactac gtaaagttct aggattcaca tcagtatgaa gtttaatcgt
    13021 gtattcttga tcatcttcta aagatattgt gtttgcataa tgcctgtcta cttctcgcaa
    13081 gaaatcatca tatttaatga tttttttagc accctcatga tcactaaact gcttttgtgc
    13141 aatggaatct aattccacaa acggtacatt taactcccca gttttataat caactccact
    13201 agctaacgct ataatttgtt ccgtatctag atcaacaaaa ttattcatat gtgtttcttc
    13261 taaattcttt tcaagtcgtt caaaaacaga ttcaaatgaa tattttttcc ctaactttct
    13321 tgcactaaaa gtacgttcga catactgact tttttctttt ctagattgtt tttctcttcc
    13381 taaagtaatt agctcatctc tacgctcata gaaatttaca tcttgtttgt aaagaacata
    13441 gccaagtttg ttatttgttt ttgaaaattg ataaacttcg atgttgtatt cttttgctaa
    13501 atgatccgaa aaacgcacaa atgaatttac ttcacaattc tccaacgtag cttcaatcgc
    13561 attttgtaca aactcttgat aattaatttt tccatttttc agtttacttg gatcgtttga
    13621 atagagattc gttctctttt ctaaattttc atagtactga ttgtctacag gttcgagtcc
    13681 attttcaaca agcacctcat cgttaatttt tcgcattcgt tctaaatctt tagggtcaat
    13741 acgcaacctc ttacaggtct ccatatcaat attattaata acaagatgac tatgaaaatg
    13801 atctttatca ttgtgacttg caacaataat ttgcgaattt ggaaaagctt tttctgcaaa
    13861 ttcgacagct atttcgtgca ttttctcaat acttaactct ttagaaatat tcttatcaaa
    13921 cgaatggata acatgaaatg cttgtgctat atcttcagat ttattcatcc tggttcttac
    13981 taaattcatt tcttgcaatg cttcctcact attatcacta caatttattc ctgagactgc
    14041 aacgcatttt tctttttttg aaggattagc tgcatattgt aatagcgagg ataaattttt
    14101 cttgcgtcct ttcgatgtta tagtagctcc caaagttgtt tcacctcatc ttttaaactt
    14161 gcaataattt gaatcgagtg aattaaattc cggtcaactt tttgggaata ctcttctttc
    14221 gtatcgggtc tagtttgctt ttctaaccaa tattgaaaat aatctttttc tacttctgac
    14281 agtttgagaa aagaagatgt actccgattg gactccaaaa aaatgtcatc tggataacta
    14341 atcatcattt tttttgctat gctattcgca agttcatctt taaaaatgtt gtaacggcta
    14401 acgtcaaaac taaaatgcag gctcttttct ttcttatttt cagattgttc tctaaaatat
    14461 ttagcaatga tatttaatgc ttttaccgct tgattcaaat tgttaccaat ttgatttaac
    14521 tgtttgatta gagctaccct atcatcttca gccaataacg gcggttcata tttgagtccc
    14581 tttaaccgct cacgacaaac atctgctcta gaaattcctc tttgttttgc aatcaatgtc
    14641 agttcaagat aagtcttttt atcgacacga aaatttaagg cctcactatg tttgcgattt
    14701 ttttcgtaca ttttttgttt atcatccatt gattatcccc tcacattttt tctagagagc
    14761 aacatttgaa attgctctct atttcatttt tcatcattaa ttcttcttga atcatcagtt
    14821 caatataatt tgtgaccgtg attttttcat ttgtcgcttt ctttactgag tcgcataatt
    14881 cggcaagttc taagaaatcc tgcgtactaa tctgtagaat taattgcatg acaatcacct
    14941 ctttattttt taaatagact atcaaacagc ggatttggtt tatcagaaat atcctgaaac
    15001 agtggattat tttcataagt aaatcgtgga cgtttcttct ccttcacttc ttgttctaca
    15061 atcacttttt cgttaggcct ttcttttcgt tcctcgataa tatttatttt ttgcgtctta
    15121 tctccctgac ttgcttcagc tcttgtttca cgtacttcta ccgttcttgg ttgatttgac
    15181 gcactacttt gtacattttc tcgtacatct actgttcgat ttccattttg actacttgta
    15241 gtattctgca agttttctcg tacatctacc gttctcggtt ggcttgtccc actactttgt
    15301 acattttctc gtacatatac tgttcgattt ccattttgac tacttgtagt attttgtaag
    15361 ttttctcgaa catctactga gcgcctatct tgactacttg tagaattttg tagattttct
    15421 ttgactgatt tttctaatcc ggtatttgaa tttttagctt cttggattct ttctttagtc
    15481 aacgcttctt tttcgtccat attagcgcct gtacgccctt ctacacgttt ccttaacatt
    15541 tcctcacgat tcattgcttc aggcgtgtat tttgcttcct gtcgcttctg attaattaat
    15601 acttcagcag acttagcttg accgtgcata gcatttttac gttcttcaat atcttgttta
    15661 actttttgct gaattgcact tgcaggatta atatttgatt cagcattatt tttcattgct
    15721 tcacgcatac gttcagaact agacataatt ctatcagcgt tttcaacttt agcgccatct
    15781 tttctatccg aaagacgtgt tggcatagaa tcagcatatt ttccagtttc atcttctttg
    15841 tttttagcta ctataggttt gctattttta ttaatagcgt acacggtacc atcatctaaa
    15901 ccttctttga atttattctt ggtatcatta gccatatcac gcatactctt aaatggtttt
    15961 gttgctcttt cactggccat tgtagctcca tccgaagcta agcctgataa tcctcgttca
    16021 cgagcatacc caagtgtttt ccccgtttta ttagcttttt gagaaagtga ttgaccaaag
    16081 ttgcgttcag gttttcgttc ttccccttcc gcttgatcgt ttccaccact atttttccca
    16141 cctttagatc ggttcatacc acctgttcct ttacgaaaca gcatagctcc catgccaaat
    16201 gctgaagcaa gctggccata gccttctttt aaaccaatat ccacgccaaa gtaacgcatg
    16261 actgttccac taccctttat cagcacaata acagcgcaga cgtacgctac tgcttttata
    16321 aaaggattta cagaagaacc gctacctgtt ccgagataat taacaaacat tgcaaacata
    16381 gacaatccga acccttgaaa tccgactgtc aaataacact gtaaaatatc tgacaaaact
    16441 tgctttgatc gttggcctgt ttccaagtca gtagcaaata ctaaaactcc aagtacgcgt
    16501 ttaaacgcta gctcgagtat tgctgtaatt ataatgaaag cactaaaaac atacgctacc
    16561 gctaaagcaa tcaatccaaa agaaatcccc catagatttg cttgatagcg ataatatcct
    16621 gatttaatgc cctcaacaaa ggtcgacaga aaattatccg aaaatttggt agcaacaaat
    16681 tcattatcag aattttcaac taattcataa cgcagattat cagctattgg gttctctttt
    16741 tccattttgt cgagtacatt atctgtcaat acttgcgtaa gatccgtttt ccaaaaattt
    16801 tcttttgtta gattgttccg gtcattcgtt ttatcgatca tggaatattg atctgtttta
    16861 ttgatataag ctaaatcagt aaccccttgt tcaatcagcg accacgccac gccttcgtcc
    16921 gaatttgtaa tcgtagtcgt atcattataa aatgttttag aaaactctat tccgctgtga
    16981 atcaacgttg gcattaaaag aatcaatgaa attgacatta caacattcat tccaacagat
    17041 tttagatcaa ccgttccctt acctgtaatc attttataac caataatcac tagtgaaagt
    17101 atcattaagc caacaacaat ggtacttaaa acagcttgat agactttgtt caatccggta
    17161 ctttctacaa atctaaacaa atctaaaact tcgggaatca tattttgaat gccatcgaca
    17221 acagcgaaca ttgattttat aacagaccag cctatccaca taccgaactg gctgtaaaaa
    17281 tttccgttgt attttttcaa ataattctgg taaagcggat tagttaaaaa ctcagtttga
    17341 ctctcagtat catcaaacca attgaaaata ctgccttctg caatgttact cgtaaaaaga
    17401 atcacagcta ttagaacaag aaaaataacc ggcaaccaat ttgtttttct attagcaatc
    17461 ttttttttaa ttattttcat tgttacctcc ctcagcccat tttcttaggc tctcagagag
    17521 tttctgagct atttttctgt caattatgat atgagttacg taatcatcat taacagcttt
    17581 taaatcgaga acaatagtat ttgagcagat actctcggtt aactcaagag tatctgcgtt
    17641 atgatcaaga cagtgaatct tatctactag atgattttca atcattttaa attccctctt
    17701 ttttttgtag cttgattagt tctaacgctt tgttttttag agaagtaaag atataattat
    17761 ttttttgttt gatacgatga tctgtttttg ctcttcgtat caagtcacgt aaagtcattg
    17821 ctagctgttc tctaacatga tctacaaaat aactattatt acaaatttca tagataattt
    17881 tcagttgaac tttttgttct ttttcagcag atttaattgc ataattgaat tttttgtacc
    17941 agttgtcgaa ttgacgataa tcactattgc tagccagttt taagcaattc acaatacttt
    18001 ctccccacat ttgtttaaga agattttctt caataaatgt ttcgatcaat tcttcatcta
    18061 atttattatt tttagttgaa agtgattcaa aaagatcatc attatgtgat tctttaatat
    18121 ctttactatc tttataagac ttatataatt ctagttcatt ttttgaacta tcagcaaaat
    18181 tgtttttgtc tcttgttttc attgatgtgt cgcatagttc aaattttgaa ctatcaacag
    18241 aatattgatt tgttccatag ttcaaatttt gaactatcaa gtttttttga ctattttctt
    18301 gatgggttat cttattaatt ttataaacat catctttgca cagtacaggc tttaacaaat
    18361 aataaaaatt tgggaacatt tgcattttat ttttattgaa gccatttttt cttttcacaa
    18421 gaagtttaac tttttgaagt tcatctagtg ctgataagat tgtatttttt gacattcccg
    18481 aaatttctac gagatttttg atttcaaata tcaaaaaaat tttttgattc tcatcataca
    18541 agttattttt caatgaaagt ctatttctat cttttagtaa catataaact aacttagaac
    18601 ttagtttcat tttcttatat ttttcattta cgtttaaaaa aataggatat tgatagtata
    18661 attctgcata tatatcagaa ttagaatagc cttgcttttc aactaagctt tcaacttctt
    18721 tttcattcat tgtcacacct gcttctgatt ttatttgcta taatagattc aaataaagtt
    18781 ttctcagaac tttattattg agtgttcccc cgatctgcaa actgacggga acactctttt
    18841 taattttttc tacttcatca ttattcaaaa aaacacctcg ttttttaaat ttgatgactc
    18901 aatatccatt ttttgcattc ttcttttgaa aacaactcca ttttttctaa taaaataact
    18961 ggcaatccca ttcttttaaa tttcaaaaaa gttgtatatg atacattcaa atatttagcc
    19021 atctcttttc cttttagaaa tggtctttct aacccggtat caattcttgc ttgatcaata
    19081 gcctcttttg tcatttcaaa aacataattt ttcaattgat tcgtttgttg tttatctaac
    19141 attactgtaa ttcctgcatc cattattttt ctcctctcat tttctatttc ttcaatcagc
    19201 caactcgcca agttgtattt atttagattt caaatcatta agtaaccaat ttaccaactt
    19261 ttgataagta gatgctctaa tactttcttt gctcccattt tcgattttga catatgtttt
    19321 ccgatttata cctatcaatt ttgctgcatc gtttttagta agtataagat cggctctatg
    19381 tcttcttaag cttctaatga attgatcatt aagagttatc attcagcgcc tccttttaaa
    19441 ttattcactt tgtggttttt atcttaataa gcacatttta aaccacgaaa cgagtaatgt
    19501 caattaaaac attactcaat ttgtggtttt tatgctataa tagtttggaa tttactatag
    19561 ggggtgttac tttgaataga ataaaatttt atattgattc cagtaaaaag accattaaac
    19621 aaattagtac agaaactggt attaactata caactcttac gaactattac cgtggtgagc
    19681 gaaatcctag aaatactgaa acatggaata tccttgctga ttatttccat gttcctgtaa
    19741 gttatttgat gggattagac gaaacaaatt ccatagaaca acctaatctt gatagaataa
    19801 aaactgctcg tttagaaaaa aaggacaaaa tagtcaagaa tattgccagt cttgactcag
    19861 attattttac tgaattatct aatctatact tttccatgac ttctttactt gaagtcaaac
    19921 ttcaagaaga tgactccgac tttctttttt taaaaagtat tctagaggac tcctttgaat
    19981 acacgattct atttgccaaa caaaagaagt ttattaataa agctttaaac ttaaatatcg
    20041 atgaaagaaa agagttagaa tctgagttat ttggcaactt aacccaagta caggttaaca
    20101 atctaaaaag agagataaca gctcgattaa tatcaaaact tcaataaaac taattattca
    20161 atcagccaac tcgccaagtt atcagattgg agagattagt tatgtcaata caaaagtaca
    20221 caacaaaaag cggtgcacga tatagtgtcc gcttgtatgt tcgtaaagat gaaaatggaa
    20281 agcaagaata ctatgtaaaa aaagggttta aaactgaaaa agaagccaag ttacacgaag
    20341 caagaaaaaa ggtcgagatt gaagataccg gctatcataa gccttctaat gacttgttta
    20401 taacagtgta tgaagaatgg ttgccttctt ataggaatcg tgtacaagaa acgacatatc
    20461 aacgtacaaa agaccttttc cgcttgcata tactaccaaa attcgggaat aagtccattt
    20521 ctaagattac tcctgttcac tgtcagaatg cagtcaatga atgggcttta aaattcaaga
    20581 atatcaaaca gcttaagtct tacagctctc aaatatttga gtatgccatt tttgcagagc
    20641 tattacaccg aaatcctatg gcaaatacaa atctaccaaa gcgggaaaag aaagaaaatg
    20701 aaaattactt ttcattagaa gaactaaaag ctttttggga tattctcaat aaagaagagc
    20761 ctttaaaaca tattttaatc ttccagcttt tgattactac tggtatacgt aaaggagagc
    20821 tagctgctct tcattggtct gacatttatt ttgaagaaca gctaatgtat gttagaaaga
    20881 gctatgcaac tattcgaaat gaagagaaga atgcaagaag aaaaactgtc aggattcaaa
    20941 aaaatacaaa aaatatatct tccgaacgaa tattaccaat tgatgcacaa actattgaaa
    21001 tgttaaaaat ctggaaaaaa gaacaagctc gagagctttt gcagtttggc ataaatacaa
    21061 atacaaaaaa tcaacttatc tttacctatg taaatgcaga cggtgacatt aatcaacctt
    21121 tgcatgcgga ctattcaaat aatatattga aacgtttaga gaagaaatat aaatttaagc
    21181 acgttactat tcatggattc cggcacactc acgctactct tctactagaa ggtggagcaa
    21241 gtatcaaaga aacacaagat cgattgggtc ataaaaatgc tgaaactacc cttaacactt
    21301 attctcatgt tactgaaaaa gctcaacgaa atgcgttaga aaaattcatt gcttacactg
    21361 gtttataata ttgtcattac ctttctcatt accagaacga aaaaaagaca ccttctcgca
    21421 atgagaaagt gtctgaaatc gttggtataa caacgattaa cgttttgaaa actgtgaagc
    21481 tttacgtgct ttcttaagac ctggtttttt acgttcgacc atacgtgcgt cacgagtaag
    21541 taagccagca cgtttaagag ctaaacggaa gtcaggatca acttctagca atgcacgagc
    21601 gataccatga cggatagctc ctgattgtcc agcgtatcct ccgccgttta cgttaacaaa
    21661 tacgtcatat gcgccttttg tttcagtaac agcgaaaggt tgattgatta cttcacgcaa
    21721 gtcagcatgt ggaatgtatt cttcaacatc ttttttattt acagtaattt tgccagttcc
    21781 tggtactaga cgtacgcgag caactgcgtt tttacgacgg cctgtgccga tatattgaac
    21841 ttgtgccaat gaaattccct cctattagat taagtttgtg atgtctaaaa cttctggttg
    21901 ttgtgctgca tgtggatgtt ctgctccacc gtatacgttc aattttgtaa attgtttgcg
    21961 gcctaaagtg tttttaggaa gcataccttt tacagaagtt tcgatcaaac gacgagaatt
    22021 tttagcacgc aattcgccag cagtgattga tttcaatcct cctgggtagt tgctgtgacg
    22081 gtaataaatt ttgtcagtcg cttttttacc tgttaatttc actttgtctg cattgatgac
    22141 gatgacgaaa tcgccagtat ccaaatgagg tgtgaaagtt ggtttatttt taccacgtag
    22201 tacagatgca acaactgttg aaagacgtcc caatggaaca tcagttgcgt ctactacata
    22261 ccatttacgt tctacttcgc ctggtttggc catatatgtt gtacgcacgt tattttcctc
    22321 caatttaagt tctgatgttt gacatacact tcgagtttcc ggggctcttt gtggggcaaa
    22381 caataccatt taacatatta cccgctaatc tagtcaatgt caacgttttt ctgcttgatt
    22441 gaaaaattta tatgaaactt tacttctttt ctttctttta tataaaaggg tcgtttcctg
    22501 tcaaaatgaa aaacaaaaag aggctgtgac aaaagtcttg tcacaacccc ttattttgaa
    22561 taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatccata aaacataaga
    22621 agctgttttt taaaattgtt tcttgtgact cggagctgaa cggcaggctt acaacctctt
    22681 ggtaaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc aaaatatgag
    22741 gaactatttt gacgtttgtt tctctaatac tcaggtcaaa acgctgattt cacaacctct
    22801 ttttcggtca agttcaacaa cagtttttac gcgtcgatct ctggcgcttt gcccaaatga
    22861 gcttggatca tccagatacg tttttctgtg gctgttttga attcgataaa gatatcttgt
    22921 gttggatcgt cgccttcttc tccagaaact tcgattcctt tttggtaaag atctgctaga
    22981 taacgatagc ctgcaacaag tgtttccaaa cgttctggaa tagtacgatc ccagcggcca
    23041 atttcatcag ggatttttgt gttatctgca aattcacgca atgtagaata aggtgaaccg
    23101 tctaatgtga tcaagcgttc ggatacttca tctaactgag cgttcaaatc atccatgaac
    23161 tcatccatca ttgggtgcaa tgtaaggaaa cctggtcctc tcatgtacca atgtgtttgg
    23221 tgaacaacaa cagacatttg gcttagatct gcaactaatt gatttaatac ttctttggtt
    23281 tgttggaatt tcatctttct tgcctcctaa cattttctta cacttaaaat aatagtagac
    23341 tttcaatgaa aagtacaaca aaatcattta cctgagaacg attttcagtt cgctttttta
    23401 tccgtaaagc caatcatttt tcagaatcag ctaattcttc tcgcagttga gcaaattgct
    23461 gataagtctg tgctaaatgc ttttgtgtcg aaacaaataa tggatagatt ttctgatatt
    23521 ttttatgatc agtttcttgt actccaactt gctctttcaa ttctatcaat tcctgtgctt
    23581 cactgatact ttggatcttt ccgatgcttc tccagcctaa aatcacagct cctaatgcgg
    23641 atgcttctgt gacagtaggg aaaacaagcg ggtaacccag tacatcagcc atcatctgac
    23701 gaaaaatcgg agattgagca aacccgcctg tagcacggat ctctgtgagc tgattttcct
    23761 ggcaaactcc ttttaaaatc cgataaaggt tgagacagat cccttctaga atcgccttca
    23821 tcatttcttt ttctgtatgt tgccgctgta aaccgataaa actgccaaac gcatctgcgt
    23881 cccaaagcgg tgctcgctca ccaagtaaat agggatgaaa aatcagacca ttggcacccg
    23941 ctggcacaaa agcgatagcc gaaagcagtg catcatatgg attttctcct ttttctttag
    24001 cagattgaac gacttcctgt aaaaatgtat gccctgccca atcaaagatg ccagcaccat
    24061 tgctagtcgc tcctcccact acccaatgtt tctgatccag aacataacaa aacgtctcca
    24121 tttctggatg gaactgaggc tgagtagtaa taaaacgaag tgctccgctc gttcctacgg
    24181 tcaaagcggc gatcgtatca ccaaaagcac ctaaccctag attagaaagc ggcccgtctg
    24241 cccctcccaa aacaaatgat atatcatttg gcaaacctag ttctgctgct gtttttgatg
    24301 gtagacctgt tacttgatag gtggaaggaa agacttgcgg caattgctct attcgaatgt
    24361 tcaagtagtc aagcacttct tctgcccatt ggaattcatg gatatcaaaa tatccagttc
    24421 ctgaagctgt tccgtagtcg ctgatcagtt cgccaaacaa tctataaaag atatagtctt
    24481 tgataccgac aaaataagca gtttttttga ataattcagg ttggctgttt tgcagccact
    24541 tgattttgtt caatggtgtc attggatgaa tcggcgtccc agtctgccga taaaattctt
    24601 tgccaagcac ggtatatttc agtttttctg cttcgtccgc tgctcggtta tctgcccaag
    24661 tgatcattcg agataacggt gttttctctt catccaacaa tatcaggcca tgcatcgcag
    24721 aggagaagga gacaaattga atgattgtgt ctgaaggcaa ctgtttaatt acttctttta
    24781 taacggtgca gacaccttga aagatcaatt cctgatcttg ttctgccatc ccttgtgttt
    24841 cttggatcaa agggtagcga aaataagcgg atgctgcttc ttgggcttca tctgtgaaag
    24901 caactgcttt ggtttgtgtg gttccgatat ctaatccgat tgaaacgttc attttttcac
    24961 ctcaaatttt atgtatgtac aaaaatttag tttaagcttg cgtatcttgg ttttgaaaag
    25021 ggggagatgg cgtgttactt tattttatca ttggttgctg tatcggatca tttttatgtt
    25081 taaccgccca gcggattccg ttaggtcact ctattattta tcctagatct cattgtgtca
    25141 aatgctgtcg ttcgctttct tggtatgaat tgattccgat tctctcgatc atcgtccaac
    25201 gatttcgctg ccgctattgc aggtgtcggt tgcccttcta ctatttgttg gcagaagcct
    25261 tatgtggtgg tctttttgcc tggttcttta ctttttcttc cgcaagaaat ttttcgacgt
    25321 tcatatggat gctttcggct ttgctatttt cgttgatgga tttattttac ttcatggtcc
    25381 attctcacac gctattctgt tcttgggcaa ttttatggat tttttggttg caggccggcg
    25441 tcttccaatg gcaaagtgtg ctgttaacgt ttatcgtagg cggtgtgtgc cttcgctatg
    25501 gtcaatccta tctcggagct ggcgatactc tgttgatgct ttcttggagt ggtggcttgt
    25561 ctctagaaga gttgcttcag atcttgtttc ttgctagtct gtttggaata tccgtttttt
    25621 ctgtttactt tatctgtcat aaaaaaaagt tggaaaagct gcctttcatt ccttttttga
    25681 gcagtgcgtt gctgattgtc cttcatctta ggtattgaaa aaaggttgtg acaaacgact
    25741 tgtcacaacc tctgatatat agtgttctct taccaaatcc ttgtgacttg gagctaggcg
    25801 acaggttcac aacctctcca ctaaatggtg ttcaatcagc tgaccctcag tattagctca
    25861 aaatgctggt tttacaacct ctgatatacg gtgttccata agtagctttt tcggcttctt
    25921 atttcttgac gccccaatgt cgacgaagga aacctgcacg tccagaacct tctcgataaa
    25981 gattgtaatg gagcttgttt ttcttgtaat aatcctgatg gtaatcttct gcaggataaa
    26041 attcgcttct tggttcgatt tttgtcacga ttggcttatc gaatcgcccg ctgtcttgca
    26101 atgcttgttt tgaacgctct gcaatatttc gttcttcttc atcattatag tagatcactg
    26161 gacgataaga atcaccgcga tcagcgaact gtcctaatgc gtcagtcgga tcggtttgct
    26221 gccaatagat ttccaccaac tgatcataag aaataacttc tggatcatat tcgatctcta
    26281 ctgcttctgt atgccctgtt gtgcctgttg ttacttgttc ataagttgga tttggtacat
    26341 gcccgccagt ataaccggat gtcacagaaa tgatccctgg ctgctcttca aatggtttga
    26401 ccatacacca aaagcatccg cctgcgaaaa ctgctttttt tgtcatataa aatccccctt
    26461 ttttctcatc ataccttgat ttccaaaagg aggataacgt tttgcttgtg acagaaaaaa
    26521 cggaaactat tatatacgtg atacggcttc tgataaatgc caaaacgaac gtacttgatc
    26581 aaaaacggtt aaatcaggaa tcacatattc agcagaagga agatttatgt tttcacctat
    26641 tcccaataca tgcatgttac cagccaaagc tgcttgacaa ccagcttctg aatcttcaat
    26701 gaccaagcat gcatttggcg gtacattcaa ttgctgtgct cctttgagaa aaacttctgg
    26761 atcaggtttt gcttttgata cttgcgtgcc gtcaataagg acatcaaaat aacttgtcaa
    26821 attcgttttt tctaaaatga ggcgtgcatt tttgcttgct gatcccaatc cgatcttgat
    26881 gtttgttttt ttcaagtact caagaacagc tattgcacca ggcaaaacgg atgtttcatc
    26941 cattttttga atcgcttgaa gatatagatt gtttttttgc tcagcaaagg ctgatttctc
    27001 tttttctgag aatttctgtt ccatttttcc aaatgacagc aatcgctcta gtgactcggt
    27061 tctggatatt cctttcagtt gctcgttttc tttctcagtg aatggaatgg acagctggtt
    27121 agctaaaacc agccacgctt cgtaatgata acgagctgtg tctacaagaa cgccatctaa
    27181 atcgaatagt gctgctttga ttttcatcga ttacctcctt gaaaatgaac gacttgaagt
    27241 aaagatgcga tgtttttagc tgtgcgatga agattatcag tggcttgctg taaagcttct
    27301 tgtaaaggca cagctgcagg aaggatcgga aaaacggccg aaatgccaac atctgctgct
    27361 ttttcgcttc ctttaccaac acttccgcaa atagctatca caggaacgcc ctctggtact
    27421 cttctagcaa tcccaactgg cgcttttcct tttagacttt gtccatctag acgtccttcg
    27481 cctacgatta cgaggtctgc ttgctgtacc cgtttatcga actccagtaa atccaatacg
    27541 tagtcgatgc cagagactat tttccctgaa gcaaatgcta acagtcctgc acccattcca
    27601 ccaccagcac tactgcccga tgcctcgatt acttgagggg caaatctcat ataaaaatca
    27661 tgcatggctt ggtcagccac tgctaattca ttttctttca agcctttttg tggtccgaaa
    27721 acaaaagaag cgccttcttt tccgcataaa agattagtaa catcagtaat gacagtgatt
    27781 ttggctttaa tcagctcaat aggcacgttc gttttatcaa ctgtagcaat tttacctaga
    27841 ttttctccca gtgcttctac tttttgatta tcttgatcgt aaaaagcata acctagacct
    27901 tctgccatgc cgatgccgcc atcgttggta ctacttccgc caacgccgat gatgatgtgt
    27961 cgaacacctt gttgcaacaa ttgtcgtatc agttctccta ctcctcgcgt ggataaccta
    28021 agtggctgtt gttggtttag tgggacatct ttcaatccga cgatttctgc catttcaaaa
    28081 atcgcagttt gaccgtctga agcgtactgg atttctttgt ttttgccaaa tgcattagta
    28141 acgactgctg tttgccgctt caaccttaaa ctgtctgcta atgatgaaac cgttccttct
    28201 cctccatcgc ctataggtag cagatcatat tcagcctgaa caaaaacttc ttgaaaacct
    28261 gattgaatgg cttgtgctgc ctctaaggct gtcagacttt ctttgaaaga atctggcgca
    28321 atcagaatac gcattatttt cccctccgtt ccaagatcag ttctttttcc agctggtatt
    28381 ggtctccatt gacaaaaata ggtaatgttt ctttcgtctc tttttttatt actatttgtt
    28441 tttttgtcaa aacaaatagc aacctgtgat ttttccaaat aaaaggaaaa gaaatttttt
    28501 cccatgctga aggaagtctt ggagaaatag aaagagttcc attcgttact gtaatacctg
    28561 aaaaaccaaa tataactgct agccatagtg ctcctaatga agcagcatga attccgtcat
    28621 ctgatgaatg cggatctggt cctaaatcaa tcaggcacgc ttcttggaat aaatgatacg
    28681 cccattccat ttcttggata cggcaagcct ctatggcgtg aatcgcttta ctcaacgatg
    28741 agtcgtggat tgttttgttt tcatagtaat gcaaattctt agaaacgatt ggttgagaaa
    28801 aatactcagg taataagtat aacagcatga caagatctgc ttgcttgctg acttggagtt
    28861 catttacttc gctacgggca taatcaagta aaatagactg gctgccagga cgttctttgt
    28921 aagtggtcaa atcaataact ggcttactga gatacgtgtc atcttgcgga atcagctgtt
    28981 ctttgttagg cactggcaaa tataacttat ctaaaaaatt ttgacattct ttcaaaaaaa
    29041 gcggatcgcc agcttgatat ttaacagcta aggccacatt ataatgcgcc agatagttcg
    29101 tatatgcatt attgtcgata tattctgtgt attcatctgg accaatgact ctgtgtatct
    29161 ctaaatgatc atcgactttt acagctctgc tcagccaaaa agctgctgtt tcatgaatga
    29221 gcgtttttcc ctttgtttct aaaaacgatt gatcctctgt tgcttggaca tactcttgaa
    29281 cggcataagc aaggtctgca ctgatatgat gttctgcggc agctgaagca acttcttgtc
    29341 ttgttccggt tttgatattc aatgcagcaa atttgggtgt ctcttcctga ccagtgtgtg
    29401 cgctttccca tgggaaaagt gcaccttgat agtgataatc tcttgctttt tgccgtgcgc
    29461 cttctaatcg atcataacgg tacagcagca aattctttgc tacttcagga aactgatata
    29521 aaaagaaagg caggataaaa atttccgtat cccagaaaac atgcccttta taaccttcgc
    29581 cagttagtcc cttcgcccct acactaacac ttggatcatt gcggggagtc ataatctgta
    29641 aatggtaagc agcgaagtcc atcgctaact gatcgaaaga agaatcgcct tggatcgtta
    29701 tccgatgctg atgccaaaaa tctgtccaat aagacatcga ctgctctaaa atttctgtat
    29761 aactaacgct tttctgggct tgggcaatac ttttcttaaa cgggtctgtt tgctgaagag
    29821 aggtagagag cacgatgatc ttctccagcg taaaggacgt attttttctg atcttttctg
    29881 aaaaggaagc ggtgatccgg cgattttttg cttggaatac acctgcttta ttgaatttag
    29941 ctgcaatccc aatatcaatt ttactttctg ttgtctggta ggatgcactg atcaaatcgt
    30001 caccaaatac tcgcagttct ttttcaacca actgctgtgt accaaaattg gtttgctgtc
    30061 catcgatacc cgttttcaca tctacttggc agtcttcggt caacggcttg atgaccactt
    30121 tcagggctgc ctgatggaga ttattttgat cagcaacacg acaagtaatg acttccactt
    30181 tttttccttg agcagtttca atgagcgcat agcgtttaag ctctcccatt tctaaatcta
    30241 gtgcccgctc gtatgcaaga actttcatcg tctcaagaga aaaatgttca ccgtcgattt
    30301 ttatttccca tctgatgaca tctgggagat tcactaactc ggctgattct gctcctaaag
    30361 gtcggttata tacaccagct gcaaacattc cccttacttg attccgatag tcttcctcct
    30421 ggcttcctct gataccaaaa aatccatttc caatggtcat ctgactcgca tacttattga
    30481 tatactctaa atcaaactgt ttttcttcta ctttcataag gtaataactt ctcctgtttc
    30541 tgccgaacga tataaatttt ccaccagatt ctggatgaca tatccttgcc agccatctgc
    30601 aatcatctga gtctggtcac cgagcgcccg attcacaaaa tgagccatac ttagttcatg
    30661 tgggtcatta actggcgtgg tcttttgtaa ggaaaccagc tttccatcta catcttcgaa
    30721 tagttcgcct ggatacagat tagcacctgc ctgatcgcca caaaaagcca cattcagttt
    30781 gtcttctctg atattcaaag caaacgaagt attcagttgg atgatcttgc cgttttctaa
    30841 ttccagtgtg ccaaaaattg catcttctac tgtgaatttg cttggatccc atttcccgaa
    30901 tttccctgaa ttttttttcg taccaatttt ttggaagctg tacgcccgaa cgctttttac
    30961 tttaggaaat ccagtaatat acatcgctgt atcaagcatg tgaatgccat aatcgatcaa
    31021 aggcccgcct ccttgaagtt ctttgtcgat aaacgttccc cagcctggaa caccagaccg
    31081 acgaagcgca ttcgcttcta cgtaataaat atccccgaga acatcaagat gattcttgat
    31141 cattgttgct tcgtcagaaa aacgatgctg gaatccataa gccaaaatct gcccgttttt
    31201 atcggctgtc tctgccatct ttcttgcttg atcagctgtc atagctggtg gtttctcgca
    31261 catcactgaa gcattagctt cgagggcagc caatacatgt tcgaagtgaa agcgattggg
    31321 tgtacagaca ctcacgatat cgggagaaat ctcattcagc atttgtgtaa ctgatgtata
    31381 cgcatgagaa aatcctgtac gtcggcaaaa gtcttctgct cgctggctga ctggatcaac
    31441 cacagcggcc aattctaaga catctgatct tttttgataa taagcagcat gtactttttc
    31501 tgctacttgt ccagcgccta tgattgctac tttcttcatt tatttctctc tcctttgctt
    31561 atttacttat tgttattctt tctctattct ttctctaaat catttaaatg acgcgctgtt
    31621 tccagacagt ttttcaagta ttgcaatgat tcctgataaa ttttcacttc attttctcct
    31681 cgcaaacgac attcatatac catataacct tcatactcat tttgaagtag cgtttgaaaa
    31741 tggcgagtaa aatcaattgc gccagatcct ggttggtagc gatgattatc tgctaaatgg
    31801 atatgaccca catactcttt ttgcgtagtc aatgcttttg aaatatcatc ttcttcaata
    31861 ttcatatgat aaaaatcagc gatgatctgc acatgtttca attgattaga gtcaatgtaa
    31921 tgacgcgcat ccgctaacaa attgatcata tggtcttggt atcggttcaa aggctccaaa
    31981 taaataaccg tacctgtttc ttcggctact ttatctaaat aacgaagtga ttcgctgact
    32041 gctttatagt ctccttcttt gcttcggggt gaaaccatcg gcggtaatcg ataagtgaac
    32101 attccccaag ctgctgggat aacgattcct ttgcctccaa cctcgcttag tgcacgcaaa
    32161 atctcagcaa tttgacgtac accattaaga cgccgttctt cgataaagtc accgatccac
    32221 ccatcgtagc cgttacaagc tgtcactact gggatccctg tttctttgat tgcctgtttg
    32281 actgtgtcga tattttctat taacaactta ccgtcgattt cgtattcttc aaatccacag
    32341 tctttgataa atctaaattt ttctaaaatg tctcttggga aaaatgcttg atcttgcgta
    32401 ccgaatttca ttgttctcct cctcggactt gaacgcccat tttgatactt tcttctgggt
    32461 gctggtctac ataacgcatg taagcttctg cgctttctgc aaatggcaca actggatcaa
    32521 tgatttcttc acaatccaag taaccgttca tcaataattc ccaacatgtg tcctcgattc
    32581 gtttacggtt ccatcttggg taatctgggt ttggttcact agatgctcga gcaaagacga
    32641 tttttgcatt attgaaatgt gcttcgcgac caagattaaa cccagctgga aaaggtttag
    32701 caaaggcaac ataagaaatg atcccgccat agccgatccc tattaaagaa gcttgtaatg
    32761 cggcagcact gccactcgtt tcaataactg aatctactcc acgtttatct gtcaattcct
    32821 ttagacgaaa accgacatct gctttcatag gatctagcgt gtaatcggca ccgtgttttt
    32881 ctgctaattc tctacgctgc gctatgggat cgatcccaac gatggtagta gcacctgctt
    32941 ttttagctaa ttgaatcgca atttgcccga tagcgcctaa accgaagatt gctacagcat
    33001 cacctgctcg aacatgtgct tcccggatac cacctaaagc gaattgagca ggatcgtaac
    33061 atactgcatt tttccattga tccaaccgat caagtttacg tagtttataa ttatcaacac
    33121 cgtttgtaat cagcgtttca cgaatgggtc cataagtgca gactaactct cctttttgat
    33181 actctgtcac caaactgccc gtttcttcga tcgtaccgac aatcatattt cctaattgaa
    33241 attcgccaaa taccacaccg cgttggcttc cttcttctct tggcaagaat aggcgccatt
    33301 cttctgaaaa ttcttcatcg ataaatggac ttaatcctct aaagtcgaca atttctgttc
    33361 ctggttttgg agtagcgaaa attactcgga tacgtacttc gttcttttcg atctcacggt
    33421 cttcgtaatc tttgaatccg gctattcttg gttctaatgc aactaatttt ttcatctttt
    33481 ctttcctctt tcttttcttt ttatcctttt acgccgcctt cgactaaatc acctttaata
    33541 aatcgttcag ataacgcata cataatgatg actggtaaag cggtcaccaa tgaagcggcc
    33601 atcattcgtc cccaaatgta gtctggtgta ctgaataatg tgttcaaacc gattggtaaa
    33661 gtaaattttt cggaggtcga caggaaaatc gaagcaaata aataatcatt ccacgaaacc
    33721 ataaagcaat aaacgaagac agaaatgatt ccagaaatag ccaatggtat aatgatccga
    33781 ataatgatct ggatccggct aagaccatcg atcattgctg cttcttcaat atcagtcgga
    33841 atcgtgtcaa aataactttt tagcatgaat atcgcagtag gaagtgtttg aacgaccatc
    33901 gtgataatca gtgctgtatt ggtatcatac aaacctagac cggaaatgat tttaaataat
    33961 ggaacaacga tcaaaatacc ggaaaacata taaacggtgt aaaagctagc attgattttc
    34021 gagcgagctt tgaagcgtag ttttgacaaa gcgtatgccc cgctgatccc aatcactact
    34081 gcagttacgg aggctatcaa ggagatcact aaactatttt tgaaatacat gatgaatgga
    34141 aaaatctcag cattgaaaat atcctgataa tgttcaaaag tccattcttt cggaaaaata
    34201 gtttgtgtcg ttgcaattgc ttcttgtgaa tccttgaatg atgtcatcag catgatcaaa
    34261 aatggaaaca aattgacagc taatagcaac aaaatcgtcg cgtaaaataa gattttcctg
    34321 ctctttttat ttttcatttc aagtccacct tctttcgtga gagcaagacg accgctgcaa
    34381 taatgatgaa caatagaatc gatatagctg ctgcttttcc aagatcattg aacgcaaagg
    34441 ctgtctgata caaataaacg cctaagatat tgactttatt cgtcagtaga tagacatctg
    34501 taaacatata gaacatccag atagatcgta aaacaatcac agttgctagt actggtttga
    34561 ttgccggcag tgtcacgatc ttgaacttct gccaagcatt tgcaccgtcc atttcagctg
    34621 cttcataaag cgttttgtcg actgtttgca aaatagccaa aaatgaaata aacgcatacg
    34681 gatagtatct ccagatagca aacaaaacga ccataaagaa gctagcaact ggctggtcga
    34741 accacagcgg tgcttcgttg aaaagctgca acctatctac taacagatca ttgacgattc
    34801 cataactatt attgaacata tatttccaag caaaaatcaa agaaatagat ggcgtaacat
    34861 aacttaataa aatcagtgaa cgcaccgttt tcctaaatct gaacttgcgg ttaagcaaaa
    34921 tagctacggc taatcctaaa gctgtactgc ctgctacaac taagaatgtg taagcaactg
    34981 ttaaacctaa agaacgataa aattcactat cacttaaaat ttcaagataa ttgcttaatc
    35041 cgataaaaac ggcgtctgag ttaggattca gcggcatatc gaaaaaactg atctgaatat
    35101 tggaaagcat tggaatcagc actaaaacag ctaataaaag catgcttggt gctaaaagtc
    35161 ccatggctaa ttttaagtca gaatgtttcc ttggacgatt cattgaaacc tccccctgat
    35221 aatctgccaa tagctatcta cttttttcga acaccattgg cagaacttct tgttatttcg
    35281 tatttttttg tgcgtcagct aaagcttgat cgacatcttc gtttccgaca gttacttggt
    35341 tcactgcttt tccgatcaca cctgagcttg tgatatctcc cattttcgtg aaattctttt
    35401 ccccgactag tccaaagact tgtacttcat caaaagcact agcaatctca gatggcagct
    35461 cgccaaaaga cttgatgact tcattttctt tgtaaccatc cagttcgact acttgtttat
    35521 tcactggttg agcaccacca ggagacatca agacccactt ttccatgttt ttcggttgag
    35581 ataagaaagc tgtgaatgct tcagcagcct ctttttgttt ctcgtctaga ccagaagaaa
    35641 tcgtcaatcc agatactgtg ccatagacag cttgatcttt ttgtgttggg atagcaaaac
    35701 cgatattttc tgagttcccc gcttcataaa cagaaggcaa aatgtaagtt gaatagattg
    35761 ccattggtgc tgttccattc atgaaagcat ctttgatttc tgtcacatca ttagagcctg
    35821 gcatcgtgta ttgggataag ttttgataaa acgacaatgc ttctctcatc ttttccgtat
    35881 cgatcgttac ctcgccttca tcatccaaaa catttgctcc atttgacaaa gcaaattgag
    35941 agaacgcttg ctcactcatt gttccttcag cagtaggcat agcaattcca tattgtttat
    36001 tctctttatc tgtgaaatgt tgagcaattt ttaaaatatc atcccaattt tctggttctg
    36061 aaaaaccagc ttctgataat ttttctttgt cataccaaat accttgaacc cagccgctaa
    36121 ttggtgcagc aatatagctt tttccatctt ctgaacgaac aaggtttttg gctccgtcat
    36181 aataattatc ttctccagct tcctcgataa cgttttggac tgccgtctga tcaatcagtt
    36241 catctttatc catcacttta gcaaaatcct gactgacttc cattacttct ggtaatttac
    36301 cggaacgagc taacgtaacg atttttgtat tgaacgctga ttcttctacc ggcacttgct
    36361 tgattttgat gtctggattt tctttttcaa aatcagcaac cagttcattg atcacactta
    36421 atcgttcctg ttcgacagaa gagtgcatga attgaatcgt tgtctgttgt gattcgttag
    36481 agctttgact ttcaccacaa gcagtaagaa gaaatccagt agctagcaga caggtagtaa
    36541 caataatttt tttcattttt ttccacctct tattttatta ttttcagcca catatattca
    36601 tacggctgga atatcaactt tcgttcttga ctgcaatgga taatttgtcc agaaaacagg
    36661 tctaggtaat ctccttttgg taaaagataa cctgctgatt gattggaaaa attatggata
    36721 actaagactt tctcttcctt cgcttcgcga ataaaactga taaaagaatc tgctgagtcc
    36781 agtaagtaaa tcaaactatt cggatggaag gctttttctt tttttcgaat agatagcatc
    36841 tgggtcaatt caccatatac tgctgttctt aatggatctg tctgcaactc tttctcaata
    36901 tctgctaaca aaaatttttg ccgattgatt tttcgattct ctcctgtttt ttctacgcct
    36961 ttgtaatcat ttcgacttcc cagcacactt tgaatataaa ttgccggcac gcctggtaac
    37021 gttaccaaaa atgaatgagc agctaaaaag cgtttgcttc tccattccgt cgtatctgtt
    37081 ggagaactta acgcatctag atacgtagcg tttatctcat atggactggt cgttccatct
    37141 gggtttttct tgtagtttac tcgggcaccc tctttcttca gctgttccac taattcaagg
    37201 atcgtttttt ccggcagaat acctcgtacc ggattcaatc cgataccgtc atgggaggct
    37261 aaaaaattaa aaaaagttgt ttttttcgat gcgaaatcga tggtttttaa ccattctttc
    37321 aacttacttg tattacccga acgaatcgct tgaagggtca aaggtggtag cgaaaactga
    37381 tagaccatct gtgcttcatt ctcgccattc ccaaaataac tgatattatc tttgtgtggc
    37441 acatttgtct cagtgatcaa taccgttcct ggagctgctt catcagcgat tgcacggaat
    37501 aattggatga ttttatgcgt ttctggcagg tgtatacaag aagttcctag ttctttccat
    37561 aaaaatccca cagcgtccaa gcgaataaat gaagcgcctt tttctaagta aaacaaaaga
    37621 atatcgatca ttttcaataa aacttctgga tttttaaaat tcaagtctac ttgatcttcg
    37681 ctaaaagtcg tccatacgtg tttttctttc ccgtttttgt caatgaacgt tgtaagtaac
    37741 gggctggtcc ttggtctagt cacgctgctt gtatctgtat ccagtggcaa atcaataaaa
    37801 aaatctttgt acgtatcatt caagtttaaa tattcctgaa accaagtact ttcggcagac
    37861 atatgattgc agacaaagtc gaacattaac cgtgtttcgc ttctcatttc ttctatatct
    37921 tcccatgtgc ccaaacggtc gttgattgcc tgataattta caacggagaa accatcatca
    37981 gatgtatatg ggaaaaatgg caagaaatgt acaactctaa atgtatctgc caaatacttt
    38041 tgataaaatt tcttaaatgt acgcaaagtt ggttgctgct cttcaaaaaa ttgatcccca
    38101 taagtaatca agaaaatatc ttcttcgtcc caatgcagtt tgcctgcctg gcctttcttc
    38161 tgctgataaa cactgacttt ttgttcaatc atagaggcgg ttttctcaga caaatctgag
    38221 tcgcgataaa tcgcttgtaa aagttttgta tacatcggat tcccctttct tttttgggag
    38281 cgttcccaac gatttgatca tattatggaa aacgcttact gtcaatagca tttttttaaa
    38341 taattttgat atacacaaaa atacaaataa gaaagcttat gtatcaataa ttaatagctt
    38401 cttaaaaaaa tatggtctct catgatcatg caagctattc ctaaattttc aaaatcatag
    38461 acaaaaaaga atgtctattg tactatggaa ttattctaaa acgctcccaa aaaggaggtg
    38521 catataaaat tgaaacctac tatttatgac attgcgaaaa aagctaatgt ctcaaaatca
    38581 actgtctcca gagtattaaa taaccagcca aatatctctg ataaagcaaa agcaagtgta
    38641 cttcaagcaa tcaaagaatt gaattatgaa ccgagcaaaa ttgcaagagg actgtcagga
    38701 aatggttttg acgcgattct agcattatct catcgtactt ctcatacgac ctatggcaat
    38761 cctttttttt cagatgttat tcaatccatt tctgccgttt ctgaacaaaa taacttcgac
    38821 ttgattctac aaacggcaaa aacaccagaa gaagagctag aaaaatgcgc gaaaaagatc
    38881 caagaaaaaa tgatccgagg aattattatt ttaagctcta cgatggatga aacacttctt
    38941 tttgaattgg atcaatacaa tattcctatt gtggtaatcg gaaaagtcgg aaacgactac
    39001 acgaacatct actcagtgga cacggataat ttccaggata gttacgattt ggtggaaaca
    39061 ctgatcaaga atggacaccg tcatatcggc tgtctccatg ctccgacaga agtttatgta
    39121 tccaatgatc gtctcaatgg ttaccgtcaa tgcttatttg atcaccacct agatatccgt
    39181 aatgaatgga ttatagacgg cggctttcgc ttagaagata gcatggcagc aatgaatgat
    39241 ttaatgaagc tacgagaaag accaactgcg attttttcta ccgatgcatt aaaatcattg
    39301 agtatttaca aagtggcaga acaatctgga aagcagatac ctgatgatct ttcgatcgca
    39361 ggattcagtg atcccttatt gtcctcccta ttttcgccac agttgactcg gatagacatt
    39421 ccgacaaatc agttaggaat caaagccaca gaacttttat tccaactaat ccaccaccaa
    39481 taccctgaag aaaaaagaat cattctttca accgagatta ctctcacaga ttcgatccaa
    39541 caacaagaaa tgtagaacag aattataaaa gaacagaaac actgaggata agtcagttac
    39601 tctgttcttt tttcaatcac tcatccctcg ttaccccctc ccggtatttt agcgaatttt
    39661 tcttattttc catctgtttt agatagctgt ggataactat aatgtttgtg ttaaactatt
    39721 ttttccacag ggaagaaaaa actattttta accacgttgt gtataagttt ttatagttcg
    39781 tttttttaaa cacatttacc cttatttcaa tccatgttat ttggggatac ttatctctaa
    39841 aacataaata gaataacaga catgtctact ggttttgttt tattcgagga attattcaat
    39901 aatagctagc aaaatattaa aaataaccgc tctatcttcc catcaattta ttttgcattc
    39961 aaaaaatgat ctatctttac ttttgttgat tgacttctct tggaagatga tcgttccgat
    40021 accttaattg gatacgaacc gatgttgtta agatcctttc ccacttttct tctagttaga
    40081 caaattattt tagacaaacc aaaaaagagg ttgtgacaat aatttgtcac aacctctttt
    40141 ttaaaatatc tattttgttt gattgttcat tgcgtcttct gcagccattt gcgcttcaat
    40201 atctgaaata ctgtagacat tttcgcttgc tacaccgact tcttttggtt ccatgtcacg
    40261 ataacgttgc ataccagtac cagctgggat gatcttacct ataataacat tttctttcaa
    40321 accaagaaga ggatctttct taccgcggat cgcagcatcc gtcaagacac gagttgtttc
    40381 ttggaatgat gcagcagata agaaactatt tgtttctaat gatgcttttg tgatacctag
    40441 taagacaggt cgagaagttg caggaacgcc tcctgagact agcgtattgt agttttgatc
    40501 tttaaagtct gcgatgtcta ataatgtacc tggtaagata tctgtatcac ctggatccat
    40561 tacacgaact ttacgcagca tttgacgaac cattacttcg atatgcttgt cgccgatttc
    40621 taccccttgc atacggtaaa cacgttgtac ttcacgaagc aagtagtttt caacagataa
    40681 tacatcgcga acttgcaaca attgttttgg atcgattgat ccttctgtca atggtgcacc
    40741 acggtggatc atatcgcctt ctgctacttt catacgtgct gtataaggaa cagtatacgt
    40801 acgtgtatct gtttttcctt tgattgttac ttctttggta cgtgttgcag gatcttctga
    40861 gatatcgatg acatcccctg ttacttctgt gatcactgct tgccctttcg gattacgtgc
    40921 ttcaaagatt tcttggacac gaggaagacc ttgagtgata tcgtctccgg caaccccacc
    40981 tgtatggaat gtacgcatcg tcaactgagt accaggttca ccgattgatt gagcggcgat
    41041 cgtaccaact gcttctccga cttcaacgtc agagccagtt gcaaggttgc gtccgtaaca
    41101 atgtttgcag acaccatgtt ttgtattaca tgtgaagaca gaacggattg tcactgtttc
    41161 gacacctgca tcaacgattt gttttgcgat gtcttcggaa atcaattggt ttcctgcaat
    41221 gatcaattca tttgtttcag gatgacgtac ttctttacgg gtgtaacgtc ccactaaacg
    41281 atcttctaat gattcgatga tttcatttcc ctcgcggatg gcttggattt caagacctcg
    41341 gtcagtaccg caatcttctt cacggatgat tacatcttgg gccacgtcaa ccagacgacg
    41401 agtcaagtaa cctgaatcgg cagtcttcaa ggctgtatcg gtcatccctt tacgagcccc
    41461 gtgagttgag ataaacattt ccaagacgga tagtccttca cggaagttcg aaatgattgg
    41521 caattccatg attcgaccat taggagcggc catcaaacca cgcattccgg caagctgtgt
    41581 aaagttggaa atgttaccac gagctccaga atcagacatc atgaagattg gatttttcgc
    41641 ttccaaacct tccatcagtt tttgttggat gctgtctttt gcagcattcc aaacagcaat
    41701 gacacgttca taacgttcgt catctgtgat cagaccacga cggaattgtt ttgtaatagt
    41761 ttcaacttgt ttatgggcat tttcaatgat ttcttgtttt tcattcagta cactgatgtc
    41821 agcgatccct actgtgatac cggcatgtgt cgaatgtttg taccctaagt ctttcatacg
    41881 gtcaagcatc ttagaagttt ctgttacttt gaatcgtttg aagacttctg cgatgatgtt
    41941 tcctaagttt ttcttcttga acggtaatac taattcttgc tctttgatat gtgcaggaat
    42001 atcagtacca gcttcaacaa agtatttgtc cggtgtttgg acggtcaagt tgaagtctgt
    42061 tggttcattc agataaggga attcaggagg catgatctcg ttgaagatga tcttaccaac
    42121 tgttgtgatc atcgtacgtt ctttttgcca ttcagtaaat ggtttttcac ctaatgagtt
    42181 agggttcaca ccgatacgtg aatgtaaatg aacatatccg ttgcgccatg cgataacggc
    42241 ttcgttttga tctctgaaga ccattccttc gccttcgcgt ccttcttctt ccattgtcaa
    42301 gtagtagtta cctagaacca tatcttgaga aggagtaaca actggtttac catctttagg
    42361 gttcaagatg ttttgagcag ctaacatcaa catacgtgct tctgcttgtg cttcttcatt
    42421 caatggaacg tggacagcca tttggtctcc atcgaaatcg gcattataag cttcacatac
    42481 aagtggatgc aaacggattg cgcgtccttg tactaaaact ggttcgaatg cttggatacc
    42541 aagtcggtga agcgtaggtg cccggttcag caatactggg tgttccttga ttacgtcttc
    42601 taaaacatcc catacttcgt cttcttgacg ttcgatttta cgtttcgcat ttttgatgtt
    42661 tgaagctagt tcgcgttgaa caagctcacg catcacgaat ggtttgaata attcaatggc
    42721 catttctttt ggaagtccac attggtacat cttcaagaaa ggtccaacaa cgataacgga
    42781 acgacctgag tagtcgacac gtttacctag caagttttga cggaaacgac cttgtttccc
    42841 tttcaacata tgagaaagag atttcaatgg gcggttacct ggtccagtaa ctggacgtcc
    42901 gcgacgaccg ttatcgatca atgcatctac tgcttcttgc agcatacgtt tttcattttg
    42961 aacgatgatt ccaggtgcat tcaagtctaa caaacgtttc aaacggttat tacggttgat
    43021 tacacgacgg taaagatcat tcaagtcact tgttgcaaaa cgtccacctt ctaattgaac
    43081 cattgggcgt aaatctggtg gaataacagg gataacgtcc ataaccatcc agcttggttg
    43141 atttcctgaa gcgcggaatg cttctaaaat atccaaacgg cggattgcac gtgtacgttt
    43201 ttgtccttgt gcagatttca attcttcttt caattcggct gcttctgcat ctagatcaac
    43261 attatcaagt aattgtttaa tcgcttctgc acccatagca gcttggaatt cttgtccata
    43321 ttgttcacgt ttttcgcgat attcgcgttc tgtcaacaat tgttttttct ctaaattcgt
    43381 atttcctggt tcgatgacta catatgaagc aaaataaatg atttcttcca aagcgcgtgg
    43441 gctcatgtct aatacaagac ccatacgtga aggaatccct ttaaagtacc agatatgtga
    43501 aacaggagct gctaattcaa tatgtcccat gcgttcacgg cgaactttcg aacgagtaac
    43561 ttccactccg catcgatcac aaacgattcc tttataacgg atacgtttgt attttccgca
    43621 ggcacattcc cagtcttttg taggaccaaa aatccgctca cagaacaacc cttcgcgttc
    43681 aggttttaag gtacgatagt taatcgtctc aggttttttt acttcaccgt aagaccagct
    43741 tctgattttt tcgggagaag ccaaacctat ttgcatactt tcgaatttat ttacatcgat
    43801 caaaaggggt tccctccatt tcatttaatg gcaaaccgca agacggctga tcttcgaccg
    43861 ccttgcagct catagaccgg attagtctaa attatcttct gcggtttcaa agttttggat
    43921 gacatcgtct ttttcatctt ccacttgttc agcagctttc ttttctaatt ctttggcagt
    43981 ttgctgttcg gcaaattttg tcaaagcatc aacagtgatc aaatcgtcgt cttcatcgtc
    44041 catgtcgcga agttcgatct ctgtttcttc gatatccaag acacgcatgt ccagacctag
    44101 tgattgcaat tctttcacca atacgcggaa tgattccggt acacctggtt ttggaattgg
    44161 ttcacccttg acgattgctt cgtatgtttt cacacgtccg actacgtcat ctgatttgta
    44221 tgtcaagatt tcttgtaaag tataagccgc accgtaagct tccagtgccc atacttccat
    44281 ttcaccaaaa cgttgtccac caaattgagc tttacctccc aatggttgtt gtgtaaccaa
    44341 tgagtaaggt ccgattgaac gtgcatgcaa tttgtcatcg accatgtgag caagtttgat
    44401 catgtacatc acaccgacag acacacgtcc atcaaatggt tcacctgtac gtccgtcgta
    44461 cagaacagtt ttcgcatcgc tggccatacc ggcttcacga actgtttccc atacgtcttc
    44521 atcacttgca ccatcaaata ctggcgttgc tacgtggata cctaactgac gtgcagccat
    44581 acccaagtgt aattccaata cttgcccgat gttcatacgt gaaggcaccc ctaaaggatt
    44641 cagcatgata tcgattggtg ttccatcagg taggaatggc atatcttctt ccggcataat
    44701 acgggataca acccctttat ttccgtgacg accggccatt ttatcccctt catggatttt
    44761 acgtttttga acgatataaa cacgaactaa catgtttacg cctggtgaca attcatctcc
    44821 agcttcacga gtaaagattt tcacatcgtg aacgataccg ccgccgccat gtggtacacg
    44881 taatgatgta tcgcggactt cacgagcttt ttcgccaaag atcgcatgca atagacgttc
    44941 ttctgcagat aattctgtta ccccttttgg cgtgactttc cctacaagca aatcgccatc
    45001 ttggacttct gcaccaatac ggatgatacc catttcgtct aaatctttta atgcgtcttc
    45061 cccgacatta gggatttcgc gtgtgatttc ttcaggtcct aattttgtat cgcgggcttc
    45121 tgattcatat tcttcaatat ggatagaagt gtatacgtca tcttttacca aacgacggct
    45181 catgatgatc gcatcttcat agttgtaccc ttcccaagtc atgaaaccaa ctaaaacgtt
    45241 ttggcctaga gccatttcgc cttgttccat agatggtccg tctgccagag tatccccttt
    45301 ttcgactttc tcacccaaat gaacgattgg acgttggttg tagcttgttc cggagtttga
    45361 acgacggaat tttgttacat cgtatttatc taatgcaccg ttgtcgcggc gtacgcggat
    45421 ttcagaagca tcaacgtatt caacgacacc gtcatgttta cataacaaag cagctcctga
    45481 gtcatgcgct gatttgtatt ccataccagt accaacccat ggtgcttgag gattgatcaa
    45541 cggcactgct tgacgctgca tgttggcacc catcaaggca cggttggagt catcgttttc
    45601 caagaaagga atacatgctg tcgcaacggc aactacttgt ttaggtgata cgtccatgta
    45661 gtctacttta tcaatagata cttctaagtt ttcactttga gcacgagcca taacaacgtc
    45721 ttgagcaaat gttccatctt cattcaatgg gctgtttgct tgggctacga tgtaatggtc
    45781 ttcgatatca gctgtcaagt agtcgatttg gtcagtaaca cgacctgttt cacggtctac
    45841 acgacgataa ggtgtttcga taaatccgaa tttatttact ttcgcataac tggacaaact
    45901 attgatcaac ccgatgtttg ggccttcagg agtttcgatc ggacacatac ggccatagtg
    45961 ggagtagtga acgtcacgaa cttcataacc ggcgcggtca cgagtcaatc cgccaggtcc
    46021 taaggctgaa agacgacgtt tgtgtgtcaa ttcgccaaga gggtttgttt gatccatgaa
    46081 ctgtgacaac tgagaagaac caaagaattc tttgatactt gccacaactg gacggatatt
    46141 gatcaattgt tgaggtgtca atgtttcagt atcttggatc gacattcttt cacgaaccac
    46201 acgttccata cgtgctagcc cgatacggaa ttggttttgt aaaagttcac cgactgaacg
    46261 gatacgacga tttcctaagt ggtcgatatc atcgacattg ccgatacctt ccatcaagtt
    46321 caagaaatag ctcattgaag caatgatgtc ggctggacga accgttttta cagctgcttc
    46381 tggatagccg tttccgatca cgtttacttc acgttcaggg tctcttggtg aaaatacttt
    46441 gatgacttgg acagtcattg gatctgtgac cacaccgtct tctgatggat agtaagtcac
    46501 tgaatttagg ccattatcaa tgaaaggagc tagtgtttcc atgacttggt gagtcagaac
    46561 tgttcctttt tcaacgatga tctcgcctgt ttctggatca actaatgttt cagctaatgt
    46621 cagattcaat agacgtgttt tcaaatctaa ttttttgttt actttgtaac gaccaacgtt
    46681 tgctaagtcg taacgttttg gatcgaagaa acgagcattc agcaagttac gtgagctgtc
    46741 tgctgttttt ggttcgcctg gacgcaaacg ttcgtaaacg tctttcaaac cttcttctgt
    46801 tcttgagtcg cttgcgtttt tatgcaagtc tttttcgact gtattgcgta atgtttcgct
    46861 gtcgccgaag atttcaaaga tcgtatcatc ggaaccaaat cctaacgcac gaactaatac
    46921 ggttaaagga atcttacgtg tacggtcgat acgtacataa gagatgtctt ttgcatcagt
    46981 ttccatttct agccaagctc cacggttagg gataacagta gaaccgaatc cttcttttcc
    47041 gtttttgtcg acttttccat gaaaatagac acctggtgaa cggactaact gagaaacgat
    47101 tactcgctct gccccgttga tgatgaatgt tccttgttct gtcattaatg ggaaatcacc
    47161 gaagaatact tcttgtgctt tgatttcgcc agtctcgcgg tttgtcaaac gcaatgttac
    47221 atgtaatggt gcagagtagt ttgcatcatg cgcacgagct tctgctactg tatactttgg
    47281 ttctttcaat tcataatcta caaattccaa agatagattg ccattgaagt catcgattgg
    47341 caaaatgtct tcaaacattt ctctaagtcc ctcatctaag aaccattggt aagagtctgt
    47401 ttgaatttcg atcaaatttg gtaattccaa cacttcactg attcttgcga aacttctacg
    47461 ttcgcgatgt tttccgtatt ttactacgtg tccagccaag ctcttcaccc ctctaaaatg
    47521 tcattcaaaa agactacccg atcaattgtt acaataatat ttcaaatatt atttttaggt
    47581 aacaacagac attttttgcg gcttttaggc aaaaaaaaac caaaaataga acattctctt
    47641 gaaaccttac ttcgtgagct tctacttttg tttccttata ttaaagccga aacggacaat
    47701 atttcgtttc tgccttttct tcatcagata gtttttgcaa cttcatatac tacacttatt
    47761 ttctataaaa gtcaacactt cctggcgttt cttctgcttt ttcgcaacat tttcttaatg
    47821 ttttcaattt tatgttttca attatctgac aattcttaat tgacagacct ttttatctcg
    47881 gttatacttt agaaaattca ttttgtggag gaacttctat gcgaaccaac atcaaccaaa
    47941 tcgcaccaac acaactgaat aacttttact ttagctactt tagataggtt tgtattcatc
    48001 atgatggata caaacgttaa acgacagttt gtatctatga tggctaaaga actttgtatt
    48061 gcagcaaaaa gccacgtaga caaaatgcta agtggctgag cagtgaaaat acaaagtaaa
    48121 agtatcaagc attctacttt ttcaatttat tttccgactt ttcaatccat ttggacattt
    48181 tgtctgaatg gattttttgt ttcccaaaat taaggaggaa aaagaaatga aaaaaaatat
    48241 aatcatcgga ttaggcgctg ccctgctact agctttagga ggttgcgcac aaaaagacaa
    48301 ttccgcagag gcatcagatg gacaaaaaac aattggcgta ttgcaacttg tacaacataa
    48361 ctcgttagat gctgcctata aaggattcaa agaaggatta gcagaagaag ggtataaaga
    48421 aggcgataat ctaacaatcg attatcaaaa tgcccaaaac aatcaagaca acctgaaaag
    48481 tatgagtgag aaactcgtca aagaagatcc tgatttgcta cttggaatcg ccactccagc
    48541 agcacaatct ttattaaatg aaacaaccac tatcccgatg actgtcacag cggtcacaga
    48601 cttggaacaa gcccatttag tcacatccaa tgaaaaacct ggcggcaatg tgactggcac
    48661 gacagatatg gtgccaatcg aaaaacaaat cggcttactg ttaaatatcg tgccagacgc
    48721 caaaacaatc ggcatcatgt ataacaacgg tgaagcaaac tctaagatcc aagctgattt
    48781 ggcagaaaaa gaattgaaaa aagcgggcgt caaagtcaaa gtactgacag caaattccac
    48841 aaatgatgtc caacaagtga cgaaaagtct agctaaagat gtagatggaa tctatattcc
    48901 tactgataac acgttcgcaa gtgcggcaag tgttgtcggt gaagtcgcaa aagaaacaaa
    48961 aacacctgtc gttgctggcg cagttgatca agtcaaagac ggcggtctag caacagtagg
    49021 aatcgactac gaaagcttgg gcaaacaaac agggatcatg gcagcaaaaa tcttagacgg
    49081 caagaatcca agtgaacttc ctgtcgaaac agcgaatgat ttggaactgt atgtcaataa
    49141 agacatggcc aaagcattag gcatcaatcc agacagcatc aaagaagctg aataaaacac
    49201 aggaggattc tcatgacaaa tttgctctct cttttcttat catctgcatc gcaaggggtc
    49261 ttatgggccc tacttgcgat tggtgtatac gtcactttcc gtatcttgga cattgccgac
    49321 ttgactgctg aaggaagctt tcctttaggt gcagggatcg cggcagtaag tattacaaat
    49381 ggctatcatc cattgacagc ttgtcttcta ggctttttag ggggtgcggc agcagggctt
    49441 gtttccggac tgcttcacac aaaattgaaa attcccgccc ttcttgcagg gatcatcacc
    49501 atgactggtt tatattctgt cacatcccgt gtgatgggtg cgccgaacat ctctttattg
    49561 ggtcagccaa cgatctttca ttgggtcgag tcattcggca taacaaaaga caacgctgta
    49621 ctgatcgtcg gtctgctttt tgctttgatc gttgtatgtt tacttgtttt gttcttacgt
    49681 acagaaacag gacttgctat tcgtgcaact ggcgataatc tggcaatgag cgaagcaaac
    49741 gggatcaaca cagataatat gaagatcatc ggttatatga tttctaatgg atgcattgct
    49801 ctttctggct ccttacttgc gcaaaacaac ggctttgctg atttaaattc tggtattgga
    49861 acgatcgtaa ttggacttgc ttctattatc atcgcagaag ttttgttacg taaccagcca
    49921 ctatttctcc gtctcttgac tgtcgtttta ggtgcggtca tttatcgttt tattttagcg
    49981 ttagtcttcg aactgaatgt tgaaccaagt gattcgaaac ttgcttctgc tttcgtgcta
    50041 gtgatctgtc tttctttccc gcagatccag caaaaattcc atttacctca aaaaaagaaa
    50101 ggcggtagtg tcaaatgaat gacgtcttaa cattggaaaa tctccatcat acctttgaaa
    50161 aaggaacgat caacgaaaat catgtcctga aaggcattaa tctttcctta gatcccggcg
    50221 acttcgtgac gatcatcggt ggaaatgggg ctggtaaatc tactttgctg aacagcatcg
    50281 ccggtacatt ttctgtcgat cagggtcaaa ttttattgaa tggaaaaaat atcacgaaaa
    50341 aatctgttgt cgaacgttcg aaatccatca gtcgcgtatt ccaagatcct aaactaggga
    50401 cagccgttcg tttaacggta gaagaaaatc ttgctcttgc tatgaaacga ggaaaaaaac
    50461 gcggcttctt ccgtggtgtc aaaccacaag accgaacatt tttcaaagac cagctggttc
    50521 ggctaaattt ggggttggaa aatcgtttat cctcagaaat cggtttgctg tctggaggac
    50581 aacgtcaagc tattacgtta ttgatggcca cattacagcg tccagaactg atcttattag
    50641 atgaacacac tgcagcgctt gaccctgcta cttccaaaac ggtgatggaa ttgacagaaa
    50701 agctgatcac tgagcagaat ctcactgctt ttatggtcac tcatgacatg gaagacgcga
    50761 tccgctatgg gagtcgactg atcatgctgc atcaaggaag gatcgtcgta gatatccagc
    50821 aagaggaaaa acagcagctc acagtgcccc aattattagc gttgttcaaa caaaacagcg
    50881 gcacggcatt aaaagacgat caagtacttt tatcttcata atcagaaaga ggatgggaca
    50941 aaaaatgaac gcccaccctc ttcttttttc tctttccttc tttcgttttt gctttatgta
    51001 tcaaccgttg tttcttcgaa aactattttg ttctctcctt cttcctgata gaatggatgg
    51061 gaaaggagct tgatgccatg acaacgaaag acaaggtact tcaattattg aaaaagacac
    51121 cagattttct ttccggagaa aagttggcac agcaactgga agtttcccgg acaagtattt
    51181 ggaaagccat caaagaattg gaaaaggcag gctatcgttt tgagcacggg cctacagggt
    51241 atcgttacct gccctctgac gtactagatg caaaagaaat ccagcaagag ctacggtcgt
    51301 ttattcctga tttgaaagta caaacgatgt tgacttctga atctacgatg aaagatgcca
    51361 aactggcagc agctcaagga gattctgttt cgaagttatt tgttgctgat acacaagaac
    51421 aagctcgtgg acgttttgga cggccttttt tcgctgaacc tggacgagga atctacatga
    51481 gtctcttgct ccatccagac aaacggtttg atgaattgcc tcaatatacg atcatcgcag
    51541 ccagtgcctg tgtgaaagcc attgaatctg taaccggtcg ttctgcttcg atcaaatggg
    51601 tcaatgatat ctacatggat ggaaaaaaag tctgtgggat tttatctgaa gcagtcagtg
    51661 atatggaaag cggcaggatc agtcatatca tcattgggat cgggcttaat ttttctgttc
    51721 ctcaaaatca ttttccagct gatctccaag aaaaagccat ctctgtcttt cccgacggac
    51781 aagctagtgt cacaaggaat cagctaatca gcaaaatctg gcaagaattg ttcacttatc
    51841 tttctcaccc agaaaaagcg ttggacttct ataaagaaaa atcattcgta ttgggaaaaa
    51901 ccgtgtcgtt caatcaaaaa ggaacgactt atgtcggtct tgcaaaagaa atcggtgatc
    51961 atggcgaact gatcgtccaa acaaatgaac agaagatgag tttgtcttct ggcgaaatca
    52021 gtctcacatc tatccgtacg caataattat cagatttcac aaaaggacac ctgatctcat
    52081 acagtgtcct tttttctgaa ttctcttcaa aagactctcc ttattctatc agctgacagt
    52141 ttctctatcc tctagaaaaa ggtattctaa aaatatctat aaagcatggt acactttctt
    52201 taaatgaatc aatgcaaaaa acaagagagt catcagttta cacgatcaaa agagatagca
    52261 ggccgcttcc tttttcaatc ttatagatag accatgggta acgataaaaa caaaaaagaa
    52321 cttgcgactt attttgccgt aagttctttt ttagctagtg ccttaatctc ttaatcccaa
    52381 caaacgtctc gcatagatca cctgaccaga gtgcatagca gcatcatcaa taatagaaac
    52441 gagacgcatt cctcgtgtga ctggtggtgt ccagttctcg tcaattactt catctagctc
    52501 ggaaggttcc agccttctta agtaagaaat agcaaaatca gcagctcctc gcaagtatcc
    52561 tagcagatct tctatttgat tagcttggat ctgctttgct tgttccagac tatgcttcca
    52621 atcttgttcg ttatctgcta tcgtcatcgg aaaccggctt tcccagtctt gactatgcca
    52681 aattggagaa gttcctgcta aatcagcaat ctggaaatct aattctcgag cggtatgcca
    52741 agccagccaa gtcattgatt tgatctgcgg ggctctatct tccactggaa aacgattggc
    52801 ctcctcaata gagacgcctt caaacaaacg ttcaaaccga tcgtatgccc gatccacact
    52861 ctcgcatagc atatctaaat aatccattta ttttcctcct ttttatttat cctacttact
    52921 ttttcctaat tctaggatac ctacttatat gttactacac acccttattt tttacacttt
    52981 aacagtcacc atcatcaata gtttggagat tgaaagaaaa agatagtgtc gaactatcaa
    53041 ttgaacaaga agcaacaata tgaacgtttc attaatattc tttaaataaa caatgatttt
    53101 tcaaacgaac agtttaagac taggagaaaa agagtataat aggaaaatgt ttgaaaggaa
    53161 aggaagggga aaaatgaaaa aaagggcaag tttactagtc ctagttgctt tgttgttcaa
    53221 tatcttttcg tatggtgtgg ccgtttcagc ggaaacatac attcctaaag accctgggaa
    53281 tatttataat gatctaactg gtaactatgg cttgttagga attgctagtc aattccacgt
    53341 atttgctaaa aataagacaa ctgtgaatgc acatacagac ggaaatatag caacacatga
    53401 acttgatgca aaaaataatt ttggtacaga gcttcatgaa ggtttattga accaagaaat
    53461 aaattatata caaaaaactg attctttcgc taattcttca ggcataccta caggtagcac
    53521 aagaatgaat aaatttgtcg ttggcagtaa cgtggatgta gcacttcaag atggtcatcc
    53581 tacagtcaat ggatccaggc ttgatcactt aactccagat gatttttata aagatcgtgc
    53641 tgggcatgaa tatattgatt tcgagcagga atttgctaaa ttgaatgtag cagcaaatga
    53701 tcttgcaacg atcactccag ctaaaacata tactgctgca gattttccag acatgaacaa
    53761 tcggacaatc gatttaactg atttatcaga taccgggttt ttattggtaa atatcgatgc
    53821 agaagtgtta acgatgaata cgccacttca aatcattaat ccaaatgatc aagtagttgt
    53881 atttaatgtc atcaacagtt cttctgcatt gaacgtacag tcaccaatca aatacaatga
    53941 tcgttcaaat catgaaacag aagatttttc ggatgcgaat atctcttgga attttggaaa
    54001 tgagatgact gatttgacaa tcagtgctcc ttttcaagga acaatccttg cgcctaatgc
    54061 tactatccgc attactcaaa accaagacgg aacgattata ggaacaaatg tgattttaga
    54121 tgcagctacc aatcgttggg atcctaacga aatatttatc agcgacaatg gtacagatac
    54181 gacagacaca agcgacagta cagatacatc taccacaagt gatagctcag att
//
LOCUS       DACDYJ010000011        53103 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDYJ010000011 DACDYJ010000000
VERSION     DACDYJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.011"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(32..907)
                     /locus_tag="IWM51_000641"
     CDS             complement(32..907)
                     /locus_tag="IWM51_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9970080.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            1055..1387
                     /locus_tag="IWM51_000642"
     CDS             1055..1387
                     /locus_tag="IWM51_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9970081.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            1499..2707
                     /locus_tag="IWM51_000643"
     CDS             1499..2707
                     /locus_tag="IWM51_000643"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP9970082.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            2861..3316
                     /locus_tag="IWM51_000644"
     CDS             2861..3316
                     /locus_tag="IWM51_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970083.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            3678..3953
                     /locus_tag="IWM51_000645"
     CDS             3678..3953
                     /locus_tag="IWM51_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970084.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            3966..6044
                     /locus_tag="IWM51_000646"
     CDS             3966..6044
                     /locus_tag="IWM51_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9970085.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            6204..8090
                     /locus_tag="IWM51_000647"
     CDS             6204..8090
                     /locus_tag="IWM51_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9970086.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            8102..9049
                     /locus_tag="IWM51_000648"
     CDS             8102..9049
                     /locus_tag="IWM51_000648"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9970087.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            9069..9596
                     /locus_tag="IWM51_000649"
     CDS             9069..9596
                     /locus_tag="IWM51_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9970088.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(9659..10312)
                     /locus_tag="IWM51_000650"
     CDS             complement(9659..10312)
                     /locus_tag="IWM51_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9970089.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            10444..11286
                     /locus_tag="IWM51_000651"
     CDS             10444..11286
                     /locus_tag="IWM51_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9970090.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            11444..12340
                     /locus_tag="IWM51_000652"
     CDS             11444..12340
                     /locus_tag="IWM51_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9970091.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            12343..13056
                     /locus_tag="IWM51_000653"
     CDS             12343..13056
                     /locus_tag="IWM51_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP9970092.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            13076..13594
                     /gene="msrA"
                     /locus_tag="IWM51_000654"
     CDS             13076..13594
                     /gene="msrA"
                     /locus_tag="IWM51_000654"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9970093.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            13591..13818
                     /locus_tag="IWM51_000655"
     CDS             13591..13818
                     /locus_tag="IWM51_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP9970094.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            13970..14521
                     /gene="lepB"
                     /locus_tag="IWM51_000656"
     CDS             13970..14521
                     /gene="lepB"
                     /locus_tag="IWM51_000656"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9970095.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(14604..15146)
                     /locus_tag="IWM51_000657"
     CDS             complement(14604..15146)
                     /locus_tag="IWM51_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9970096.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(15383..15781)
                     /locus_tag="IWM51_000658"
     CDS             complement(15383..15781)
                     /locus_tag="IWM51_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9970097.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            16041..16901
                     /gene="ylqF"
                     /locus_tag="IWM51_000659"
     CDS             16041..16901
                     /gene="ylqF"
                     /locus_tag="IWM51_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP9970098.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            16894..17661
                     /locus_tag="IWM51_000660"
     CDS             16894..17661
                     /locus_tag="IWM51_000660"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP9970099.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            17717..18574
                     /gene="dprA"
                     /locus_tag="IWM51_000661"
     CDS             17717..18574
                     /gene="dprA"
                     /locus_tag="IWM51_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP9970100.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            18696..20774
                     /gene="topA"
                     /locus_tag="IWM51_000662"
     CDS             18696..20774
                     /gene="topA"
                     /locus_tag="IWM51_000662"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP9970101.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            20743..22119
                     /gene="trmFO"
                     /locus_tag="IWM51_000663"
     CDS             20743..22119
                     /gene="trmFO"
                     /locus_tag="IWM51_000663"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP9970102.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            22317..23222
                     /gene="xerC"
                     /locus_tag="IWM51_000664"
     CDS             22317..23222
                     /gene="xerC"
                     /locus_tag="IWM51_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP9970103.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            23259..23807
                     /gene="hslV"
                     /locus_tag="IWM51_000665"
     CDS             23259..23807
                     /gene="hslV"
                     /locus_tag="IWM51_000665"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP9970104.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            23821..25221
                     /gene="hslU"
                     /locus_tag="IWM51_000666"
     CDS             23821..25221
                     /gene="hslU"
                     /locus_tag="IWM51_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP9970105.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            25239..26030
                     /gene="codY"
                     /locus_tag="IWM51_000667"
     CDS             25239..26030
                     /gene="codY"
                     /locus_tag="IWM51_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP9970106.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            26112..26987
                     /locus_tag="IWM51_000668"
     CDS             26112..26987
                     /locus_tag="IWM51_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP9970107.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27119..27757)
                     /gene="plsY"
                     /locus_tag="IWM51_000669"
     CDS             complement(27119..27757)
                     /gene="plsY"
                     /locus_tag="IWM51_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP9970108.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            27884..28318
                     /locus_tag="IWM51_000670"
     CDS             27884..28318
                     /locus_tag="IWM51_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP9970109.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            28484..30529
                     /gene="parE"
                     /locus_tag="IWM51_000671"
     CDS             28484..30529
                     /gene="parE"
                     /locus_tag="IWM51_000671"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP9970110.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            30541..32991
                     /gene="parC"
                     /locus_tag="IWM51_000672"
     CDS             30541..32991
                     /gene="parC"
                     /locus_tag="IWM51_000672"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP9970111.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            33268..35499
                     /gene="pflB"
                     /locus_tag="IWM51_000673"
     CDS             33268..35499
                     /gene="pflB"
                     /locus_tag="IWM51_000673"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP9970112.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            35743..36504
                     /gene="pflA"
                     /locus_tag="IWM51_000674"
     CDS             35743..36504
                     /gene="pflA"
                     /locus_tag="IWM51_000674"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP9970113.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            36589..37515
                     /locus_tag="IWM51_000675"
     CDS             36589..37515
                     /locus_tag="IWM51_000675"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP9970114.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            37697..38590
                     /locus_tag="IWM51_000676"
     CDS             37697..38590
                     /locus_tag="IWM51_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP9970115.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNKKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            38724..38849
                     /locus_tag="IWM51_000677"
     CDS             38724..38849
                     /locus_tag="IWM51_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9970116.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(38983..39615)
                     /locus_tag="IWM51_000678"
     CDS             complement(38983..39615)
                     /locus_tag="IWM51_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970117.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(39763..40305)
                     /locus_tag="IWM51_000679"
     CDS             complement(39763..40305)
                     /locus_tag="IWM51_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP9970118.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            40440..40829
                     /locus_tag="IWM51_000680"
     CDS             40440..40829
                     /locus_tag="IWM51_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970119.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            40833..41597
                     /locus_tag="IWM51_000681"
     CDS             40833..41597
                     /locus_tag="IWM51_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9970120.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            41726..42673
                     /locus_tag="IWM51_000682"
     CDS             41726..42673
                     /locus_tag="IWM51_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9970121.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            42803..43543
                     /locus_tag="IWM51_000683"
     CDS             42803..43543
                     /locus_tag="IWM51_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970122.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(43834..44502)
                     /locus_tag="IWM51_000684"
     CDS             complement(43834..44502)
                     /locus_tag="IWM51_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9970123.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            44636..44923
                     /locus_tag="IWM51_000685"
     CDS             44636..44923
                     /locus_tag="IWM51_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970124.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            45339..46877
                     /locus_tag="IWM51_000686"
     CDS             45339..46877
                     /locus_tag="IWM51_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP9970125.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            46874..47839
                     /locus_tag="IWM51_000687"
     CDS             46874..47839
                     /locus_tag="IWM51_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9970126.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            47826..49148
                     /locus_tag="IWM51_000688"
     CDS             47826..49148
                     /locus_tag="IWM51_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9970127.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      49211..49315
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            49389..50132
                     /locus_tag="IWM51_000689"
     CDS             49389..50132
                     /locus_tag="IWM51_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9970128.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            50147..51139
                     /locus_tag="IWM51_000690"
     CDS             50147..51139
                     /locus_tag="IWM51_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9970129.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            51151..51888
                     /locus_tag="IWM51_000691"
     CDS             51151..51888
                     /locus_tag="IWM51_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9970130.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            51878..52600
                     /locus_tag="IWM51_000692"
     CDS             51878..52600
                     /locus_tag="IWM51_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9970131.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(52684..>53103)
                     /locus_tag="IWM51_000693"
     CDS             complement(52684..>53103)
                     /locus_tag="IWM51_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP9970132.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        18307 a         9116 c        11117 g        14563 t
ORIGIN      
        1 ctttcctcat agcctatctt tgtctttttt cttatagttt tttcagtaat cttgtctgtg
       61 gcttcaaata agtaaagcct tgcccttcca cttcatgcac attgatgatt gaaaggaaag
      121 ctttttcgtc aatctcgtgg acgatcctct tagcttcagt taattcacta gagctaacga
      181 ccatatagat gatttttttt gaattaccag agaatgcgcc ttctccagaa aggaaagtca
      241 cacctctttg cagaccggtc atcagtagtg gtgcaatctc ttcgcttttg ttggagacga
      301 caagtagacc ttttgcagaa tatccgccat ctaagatcga atcaatcaca cgactgaaaa
      361 catacgaaac aatcaacgta tacatcatac gtttcaagtc gatataggtt aatgaagcaa
      421 gtaagacgat gatatcaaat gccaataggg aacgccccat agtgatgccg taattttgtt
      481 ctaaaatccg agcgataata tcactgccgc ctgttgttcc acccatgcgg tagacgatcc
      541 cgcttcctac acctgcaact aaacccgcaa gtagagaaac gatcagtaga tcatgctgta
      601 aattgatttc tagtggaacc ctttgccaga tccataaaaa gaatgacaaa gaaagtgtac
      661 ctagaacagt ataggccaag gaacgtttac cgaggatctt tcctcctagt aaaatcaatg
      721 ggatgttgat taataaagtc gtataagctg gatcaatacc aaataaagct cttaagatca
      781 aggtgatccc tgagactccc ccttctgcta aatgattagc gatgttcaag taaacaagac
      841 caaatgcata aatacttgtt cctataatga taaaaagaac ttcttttact gtttttcgtt
      901 tgaccatatg aattccagct ttcaattatg tattttcaca gaatttccat ttagaaattc
      961 cttcctaaat gtagcatgga aattcgattt tctcaattgc tcttatctag gatttgcgtt
     1021 aaagttttgg taagatagtt ttggagggaa aaagatggat aaaaagcgtt ctttatacag
     1081 tatgcagcag gaagtggatg aatatatcca gcaattcaag gtggggtatt tctctccatt
     1141 agctcagatg gctcgattaa ctgaagaagt aggtgaattg gcaagagaag tcaaccacac
     1201 ttatggagaa aaaagcaaaa aggcttctga accagttaac tcggtagcag aagaacttgg
     1261 tgatgtttta ttcgttacga tgatcatggc taattcattg aatatcgact taactgaagt
     1321 ttttgaaaaa aacatggaaa aattcaatca aagagaccat aatcgctttg agcgtaagga
     1381 cgagtaaaca agatgaagtg gtcctggcct tgaagactct gaaacggact tgtcaaacag
     1441 tcaccatgca actgagtctt tatctcagga caagaaacac gcaggaaagg ttggaaaaat
     1501 gaaattaaaa caattacctc tggaatacca aaaggcaatt ccagttctga aaaaattaga
     1561 gaatgcagga tatgaagctt attttgtagg tggaagtgtt cgtgatatct tgctgaacca
     1621 gccaatccat gatgtggata tcgcaacaag cgcatttcca gaagaaatca aacagctttt
     1681 tccaaaaacg atagatattg ggatcgaaca tggaactgtg ttggtattag aaaatgatga
     1741 acaatatgag atcaccacat ttcggacgga atcaacttat caggactacc gtagaccaga
     1801 tcatgtcgaa ttcgttcgtt cgttggcaga agatttgaaa agaagagatt ttacgatcaa
     1861 tgcgtttgct ttgaaagaag atggagaaat catcgatttg ttcgatggtc tgcaagatct
     1921 agaagaacag atcttgcgag ctgtaggcaa tcctcatgag cggttccatg aagatgcctt
     1981 acgaatgatg cgcgggttgc gttttgtcag tcaactaggg ttcaaattag aggaagaaac
     2041 cttctacgcc atcaaagaaa accacgagct attaggtaaa atatctgtag aacgtataaa
     2101 tgtggaattc atcaagatgc tcttgggaaa tgaacgggcc gcgggaatcc atgcatttat
     2161 tgaaacagag tgctacattt gctgtcccgg tttgaaagaa gaaggcgaag cacttttacg
     2221 ttttgctgaa ttaccatccg tcccattgaa agaagagagc caagcatggg cattattgat
     2281 cagccaactg gatctaaaag aaaatgagat tcgttccttt ttgaaatcgt ggaagtgttc
     2341 taaccaaatg attaaagacg ttcaagcact tgtccaagga ctacgacaac gagaaactgg
     2401 agctttggaa ccattcgaat tgtacttctt aggcaaaaaa catgcactgc aagtagaaga
     2461 attgatgatc tattttgacg aaacacctca gatggatgcc gtaggagaat cctatgatcg
     2521 tttacctatc aagtcattaa gcgaattagc agtagatggt aaaatcttgc tagaagaaac
     2581 tggaaaaaaa cctggtaagt gggttagtga agctttgcaa ttagctgaaa aagccgtcgt
     2641 agaacaaaga ataaaaaatg ataaaaaaca ggttttggat tttttatcga aagaaaaatt
     2701 gatttaaaaa acactagact gtatctgctc agctttgttg gctacttaaa gtgggcagat
     2761 acgtcattag aagaaaaaac ataagatgtg accacttaca taaatttgtg ctaaactgag
     2821 gtagtacaag aataaattac atcataaagg agtcttttta atgagtaaag aaatgacagc
     2881 gctaaaattt tattttagaa atggagaaac ctggacgatc aaccgccgtc atatcggcga
     2941 tctttggatc aaacaaatca caacaagttt tggacgaatc aatggcagtg aatttgttga
     3001 aatccatccg tgtgctggat ttaaaatcga aatcttccat gaaggggacg cagttgcgac
     3061 acatgatatc aacctaggcg gactggaaat gggcatgttt aatcgcgcat tgaaatacga
     3121 agatatcgag cgaatggaaa tcctttaccg aaatggcaca cctgatcttg tgtacttccc
     3181 atatctagat aaaggaacag aaggattaga caatcaatat caatcaacaa aaatcagcga
     3241 aaaaacaggt aacttgtata tcgtgatcaa tccagatcaa cgtgtagaag atgtatacgg
     3301 ggaatttttc gaataaatcg atttttacgc aaaaatgagg ctgtgaaaga gatgtgtctt
     3361 attactactg ataggctgtc tcttccacac cgtttattca gaataaggga ctgtgacaag
     3421 atttttgtcg cagccccatt tttttgttgt gataaaagaa caagaaaggc aggaatggga
     3481 aagtaaacat ccttttgtca gaaactttta tctttcttaa gttttacttg atatctgata
     3541 ctatttggaa tagaatgagg cgttgaaaaa tttatggaaa tttctaacta gcaataaacg
     3601 atgaaacaat gaaatatttt tcatccaaaa agaactttgc tataatgatg gaagtttgtg
     3661 tataggagat ctggaaaatg agagagagtt ttgaacaaca aaaaaaactg ttgcatgatc
     3721 gctatggcgc cctttcgatg gatgaccgcc gtcaaatact ttgtaagttg cgtaaaagaa
     3781 atatattgat gtatcgtcag ttggaacgtc tgaaacatga tctgcttcgt ttggagtcaa
     3841 aacgtgtaca gtgtgaatta gaggggaacc aaacacaggt tgaagtagtc gaaaccaaaa
     3901 ttttaaagaa aaaagaacaa tttttaaaaa tgcttacaca aaataaaaaa taaaggggat
     3961 ggacgatgga atttgctgag ttagttattg tatttgcagt aacgatcacc ttatcgaaca
     4021 tagctagccg cattttgcca atgattccag ctccactgat ccaaattttc cttggagtgg
     4081 tattaggtct gacagagtgg ggacaatcaa tcgattttga acctgaattg ttcttagtta
     4141 tgattattgc accattgtta tttagagaag gagaaaaagc agatatttct tcgattctca
     4201 aaaatttcgg gacaattttg ttcttggcat ttggaggagt cattttgacc cttgtcgggg
     4261 taggtgcgac gttatcattc ctgttgccaa gtgtcccact tgctgcctgc tttgcttttg
     4321 gagcagcgtt aggtccgaca gatgcggtag cagtcagttc tctttctgga cgagtgaata
     4381 tccctaaaaa ggcgatgcat attttggaag gagaaggtct tttgaatgat gcatctggtg
     4441 ttactgcctt ccagtttgct cttggtgcgc tgatcaccgg atctttttca gcagtaaacg
     4501 cggggatgag tctagtagtt tctagtattg ggggagcttt gatcggcttt ttgcttgtct
     4561 ggttcaaaca aaaaataatt catttgatcg aaaaagcatc agcacaagat gtcacagggt
     4621 atctgctgat cgaacttttg cttccttttc tagcctatgt attggcagag gcatttgatg
     4681 tatctggaat cattgctgca gtggcagcag gtattctcca agcttctggt tttaggaaaa
     4741 tctctgtgtt tgatgcagaa ttgtcgagct tgtcccacag tacatggaca actattgctt
     4801 ttacactgaa tgcattagta tttatatttt taggaatcga attgacgcaa gtgttctcgc
     4861 cagtatgggg agacggttta tatccaaacg gtcttttatt agcgattatc gtattaatct
     4921 ctgtcatgct ttttgtgatc agattcattt ctatcagtct tttttatgta ttcaaagatg
     4981 gcagtaaaaa gttcaaaaaa cagttgaacg agatcttgat cttaacattt ggcggtgtaa
     5041 agggaacagt cagtttggct acgattttta ttttgccatt ttcaatcaat aatatgatgt
     5101 tttaccagcg gtctttgctt ttgtttctga cggcgggtgt catcttagtg actttggtca
     5161 tagggatcat tgtcttgcct atgctgacgg aaacagagga agcgaaaagc acggatttga
     5221 atgctttgat gattttagaa gaggtcgttg aagttttgcg aaaagaaata aaggaaattg
     5281 atcgtaatac aaaagaattt ttagccactg aagcagtgat agaaaattat caagaacgga
     5341 tacgtgatct gtacttggaa gatttaaccg atgatgaaaa gcaagaagta caagaaatac
     5401 aagcactgat tttgtcgatc gaacgggatg gattggatga aagttatcgt tcgggcaagc
     5461 tttcttctaa tggttatcgt ttttactccc gttttctttc tcgattcgaa cattctatca
     5521 caagccagat tttgtctttt atcggctttt ggttcattgt tgtccgtagg ttaatgcgaa
     5581 tcgttctcca tccgaaaatg ttttggcaaa gaagatatgc tgatcgtcag acatttatta
     5641 gtgatgaaga tatccaagaa atcagagaga catatcaaaa aaatactcag ttgattattg
     5701 aaagtttaga taacttgact gatatttatg atgaaacgat ggttcaattt tttatccagc
     5761 aacgcaaact tgaaggaata aaaatgaact cggggaattt gatttcttcg tggatgatcc
     5821 agcaagatag tctgtttacg aaaaaaatgc taagaggtta ttatcttgaa agaaaaatca
     5881 ttgatgaata cgaagttgct gaaaaaatca cgacgttttc tgcgaacagc tatcgcagga
     5941 atatcaattt gttagaatct tatacgatga acaaaccatc tgataacttt tctttccgat
     6001 ttgcgtttcg agcaaagtca aaaaaaacga gtccaataaa ataaaaaggt aaaaattttc
     6061 aagttttata aaaaacaaaa catatcagaa caggatcgaa tcggtcctgt tctttttttg
     6121 agaacacaaa aagaacaaat accaaaaaaa ttttcctgaa gttcactatt tttatgatac
     6181 aatatccaag ataaggaggg aacatgttga aagaattaaa agtaactgac ctcaaaaaaa
     6241 cttatggtga aaaagatttg tttgaccaga tttctttttt ggtccacgaa aaagaccgta
     6301 tcgggttgat tgggacgaac gggacgggta aaacaagtct cttaaatatt attgccggta
     6361 tcgatagcag cgacggtgat cggcaaacag tgttttatcc aactgattac cgtattggtt
     6421 atctttctca agcagctgaa ttttccgatg aattaactgt tctccaagca gtctttcaag
     6481 gaaacagccc cttgatccaa actgttcgag catatgagga agcattgatc gaactagggg
     6541 aaaacggaga agaccctgat gtgcaaaaac gttatgctaa agcagaagaa caaatgaata
     6601 aagaagatgc ttggactaca gataccaacg caaagattat tttgcaaaaa ttaggaatca
     6661 gtgaattaga taaaaaaatc agtactttat caggtggtca gaaaaaacga gttagtctgg
     6721 cgcaagtgtt gattgatgaa cccgatttac tattactaga cgaaccaaca aaccatttag
     6781 attatgaagc gatcgagtgg ttggaaaact atttgaaaca ataccgtggc gcactattga
     6841 tggttaccca cgatcgttat tttctagata gagtagcaaa ccgaatcttc gaactttcat
     6901 ttggaaaatt atacgagtat aaaggaaatt atgaagctta cgtcttagaa aaagcagaac
     6961 gtgatcgtgt agaagtagaa caagaagaaa agcgcaagcg gctttataaa caggaattag
     7021 cttggatgag ggctggcgta caggcacgtg gcaccaagca gcaagcacgg atcaaccgtt
     7081 tcgaagatct aaaggagaac ttgtaccaag taaaccaaga agatgattta gagctgaacc
     7141 tagcgacaca acgtttaggg aaaaaagtac tggaaatcaa agatggcagt taccgtataa
     7201 atgaccaaac actgctagaa catttagatt tattgattca atctcgggaa agactaggta
     7261 ttactggtaa aaatggtgcg ggaaaatcga cattgcttaa tattctagct ggcagaattc
     7321 ccctggatag cggtacaatg tctatcggtg aaacggttca tcttgcgtat tatacacaag
     7381 aaaatgaaga aatggcacca gataaacgga tgattgctta cttacaagaa gctgctgaag
     7441 aagcaaaaac agcagatggc agtcaaatcg gagtagcaga attacttgaa cgatttttat
     7501 ttccaagatt tatgcatggg acactgatag gcaaactttc aggcggagaa aaacgccggt
     7561 tatttttgtt gaaactgctg attcagcaac ctaatgtttt attattggat gaaccgacca
     7621 atgatttgga catagcaaca ttgacgattt tggaagatta ttttagaagt tttccaggtg
     7681 ctgtgatcac tgtttcccat gaccgttatt tcttggacaa ggtagcagat aagttattag
     7741 tattccaagg aaacggaaaa caagaacttt attacggaaa catgtccagt tatttactga
     7801 agcaaaaaga gacccagcag cctgctgaaa aagcgaaacc aaaaatacag agcaaggaac
     7861 ctgctgaaaa gaagaaatta tcttacatgg aacaaaaaga atgggaaacg attgaagatg
     7921 agattgcaga acttgaagaa aaaatctcat tgctccaaga agagatgaat catcaaggag
     7981 ataatttcac tcgattacaa gaattgcaaa acgacgtatc tgaaacagaa gcacaacttg
     8041 aagaaaaaat ggcacggtgg gaatacttga gtgaatgggt agaagattag gaggaaaaag
     8101 gatggaacaa gcgtatcttg acttaggaag aaaattgttg gaagaagggc atcgtaaaga
     8161 agacagaaca ggcacaggca cattaagctt attcggatac caaatgcggt ttgatctgca
     8221 aaaagggttt cctttgttaa caacgaaacg agtacctttt ggattgatca aaagcgaatt
     8281 gctttggttt ttaaaaggag atacgaatat ccgctatcta ttacaaaata aaaaccatat
     8341 ttgggatgaa tgggcatttg aacgctacat caaaagtcca gactataatg gaccagatat
     8401 gactgatttt ggccatagat gtttgacaga tcctgaattc aatgaagaat ataaaaagca
     8461 gagcaagctt ttttgcgaga agatcctaac cgatgattcc tttgctgaaa catacggcga
     8521 tcttggacac atctacggct atcaatggcg tcactgggaa acaaaagacg gtggctttat
     8581 cgatcaaatc aaacaagtga tcgaagcaat caaaaagact cctgattcta gaagattgat
     8641 cgtttctgca tggaatccgg aagatgttcc tagcatggct ttgcctcctt gtcatacgat
     8701 gtttcaattt tatgttcaag agggacggct aagctgccaa ttgtatcaac gcagtgggga
     8761 tgtgtttcta ggcgttccgt tcaacattgc aagttatgcg ttgctgactc acctaattgc
     8821 acatgaaaca ggtttagcgg ttggcgaatt tgttcacaca ttaggagatg ctcatcttta
     8881 tatcaatcat atcgaacaaa tgaaagaaca attgagccga gaaatacgcg catttccgac
     8941 tttagttttg aatcaggaaa agcagtcagt atttgatttt gagatggaag atatccaatt
     9001 agaaggatat gacccacatc cggcgatcaa agctcctatc gctgtttaga atcagaaagg
     9061 atgagaaaat gttagctgca atttgggcac aggatgaaaa cggattgatt ggaaaagaag
     9121 accgtcttcc ttggcgtttg ccaaatgatc tgaaattctt caaacaaatg acagaagcaa
     9181 atacactagt gatgggacga aaaacattcg aagggatggg tagccgtccg ttgccgaatc
     9241 gtcagacgat tgttttgacg agagattctt cctataaagc agaaggcgtg catgtcatgc
     9301 atagtgtgga agacgtgctt gctctggaaa aagaaacaga cggtatcttt ttcatcgctg
     9361 gagggtcagc tgtttatcaa gaatttctgc cattttgtac gatattatac cgtacagtca
     9421 tccatcatgt attcgatgga gatgcttatt ttccacctgt tgactggtct gattggtcac
     9481 tgatcaatac tagtcaagga gaaatggatg aaaaaaatcg ttatccgcat caatttgaaa
     9541 cgtatcagcg taatgagaat cctgtaaaaa gcgggacagg cgaattgacg atataaaaaa
     9601 gctatggata gactagctgt tatttagagc tgtcccatcc atagctttat tttttttatt
     9661 aagtaaagaa taaaacagag aagtacataa aacctgcacc taacataaca aacaaatgcc
     9721 aaacaacatg catatagcgg atagaattca agctataaaa gaatgcgcct atcgtgtaag
     9781 aaagcccgcc tgctaccatc aaaataacac cagtcattcc aagagagtga taaagaggaa
     9841 cagcagccac gacacagagc caccccataa aaatatagat gatcgtagat acttttgata
     9901 ctctctcccg tttatgcaac gtcaacgctt tatatataat gccaagaatc gcaaacaacc
     9961 agattaatac aaataaagtc caacctagcc atcctcggat actcagcaaa caaaaaggag
    10021 tataacttcc tgcaatcaaa agaaaaatcg aactatgatc gaatacttgg aacacctttt
    10081 ttgcttttgt aaaaatcaat gaatgaaaca aagtagacgt taaaaataaa agaatcatca
    10141 tcgatccata gatagcataa gagacaatat ggagggagga atgcaagtgg gcacctttta
    10201 ctaaaagaac cactagtccc gcgatactca atccagtccc aattccatgg gtgactgcat
    10261 taaacacttc atttaatatt tcatacgttc gattaaattt cgttttttcc atgaaaacgg
    10321 ctcctttact aggtgtaatt aatgaaaaat cattatttgt ctgttatact aaaaatagaa
    10381 attgtcttac atgtagttta acatgaataa ggcatcttgt taaagaaaga gagagagaaa
    10441 ataatgacaa atgtaaaaat cgtaacagat tcttcatgta cgatggaaaa aaatgtacgc
    10501 gatgctttag gcattcgtgt catgccgcta tccatcatga tcgacggaac agtatatccc
    10561 gatgatgacc aactagacgg cgaaagattt atggaaatga tggcgaaagc aaaaaatctg
    10621 cccaaaacaa gccaaccacc aattggagaa ttcatctcga tgtatgatga gctaggagaa
    10681 gacggcagtg aagtgatttc gatccatatg acaaaaggct tgagtggaac agtagaagca
    10741 gcaagacaag ccagtaatct aacaaaaacg aaagtaacgg ttatcgacag tgataccact
    10801 gaccaaggat tatcttttca ggtaattcga gcagctgaat tagcaaaaga aggtaaaaca
    10861 gtagagaaaa tccttgaaga agtcaaaaaa gtacaagaca atacaaaatt atacatcgga
    10921 atatccacac tagataactt ggtaaaaggt ggaagaatca gccgaacaac tggtttgctg
    10981 tctagtgttt tgaacatacg cgtggtgatg aatttcgccc attctgaatt aatccctgta
    11041 actaaggggc gaggaaagaa aacgttcgat aaatggttta ataatttgaa aaatgaactg
    11101 aaagaattgc caaatgtgcg acaaatcggt atttcacatg cggatgcaaa agaattggcg
    11161 caagaattca aagagggttt gcaagaatta ttcccggaca tgcatatccc tgtactgcat
    11221 acgaatccgg ttatcgctac gcatactggt aaagacgcgt ttgcaatcat gtattatgta
    11281 gattagtcga aaagaggata agccctcagc ctgaaaggct tgcagcttat tctctttttc
    11341 tttataaaat taggaaaata attgttttct tttttcataa agactacctt tttgataaaa
    11401 tttttgataa cattagaacc aatcgaatac aagaggtgaa aaaatgaaat taacgaaacg
    11461 acatttgatc ctcgttttgt tcccattatt aattgcgatc atcatctatg caggtttagc
    11521 tgcttctata ccgaaagctg gcccactaat gaaaccagta actactgtga gagaagatcc
    11581 aaataaaaaa gaaaaacttc actacatagc tatcggagat tcgctgactg aaggagtagg
    11641 agatcaaacc aagcaaggag gattcgttcc ccttgtcgca aatgatctaa aagaccgtta
    11701 taatttgacc gcggttgaaa cagaaaacta tggtgtaaat ggtgaacgaa gtgaccagat
    11761 cttaaagcga gtaaaaacaa aagaagaaat tcgaaacaat atcaaaacag ctgattttat
    11821 cacactgact gttggcggta atgatttgat gaaagttatc caaaataatt tattcgggtt
    11881 aacggtcaat tcctttaaaa agccactgaa aaactatcag gaaaatgtca cagaactatt
    11941 ggaagaattt cgatccctca ataaaaccgc acctatttac attttaggca tctacaatcc
    12001 attttatttg aactttcctg agatcactga catgcagaca attgttaaca attggaacaa
    12061 aggaacagaa acggtgacaa aaaaaatgcc gaactgctac tttatcccaa tcaatgattt
    12121 gctttatcaa ggattgaatc aggaagtagg tgtcacagaa gaaggaacag atcaaacaac
    12181 tagcacgaca gaaaatctaa atggcatcaa aaataatgtg ctctatgaag aagatcattt
    12241 ccatccaaat aatttaggtt atcaattgat ggcaaatgca gtgagagatc agctgatcga
    12301 gacacaaaac caatggttag agaaggagga tgatcaataa acatgaaaac aagatctgac
    12361 gtatcttcaa acaaaacaaa gaaaacaaat tactggaaat tcgccttcct tatccttctt
    12421 ggggtagtac ttggttcgac cgtattttta ggcagccgta tttttgctaa tcgggaacca
    12481 gaactcccag aaataccagc tcttacggaa agacaaggag atcctgtctt aacgatcaat
    12541 tcaaataaag aaaaagtcaa tcaaatcatc agctttttcc ttagtgagta tcaaaaagat
    12601 agtgatattg aatataaatt ttatttggaa aatgaagcac tactcaatgg tactttcgaa
    12661 gtactaggtt ttcctatcga tttctattta tattttgatc cgtatgtgat ggaaaacgga
    12721 aatgtccaac taaaagctag aagtctttct atcggaacat tgagtttgcc aatcaaagat
    12781 gtgatgaata tgatcaagag aaactataaa ttaccagatt ggatcgaagt caatacagat
    12841 gatctgacta tcatgcttag gttggatcaa ttccgaatgc aaaacgggat gtatattaaa
    12901 gcagataaaa tcgacttagt aaatgatgac attcggttta gtctatattt gccaaaagat
    12961 acctctgatg cttcaaacaa ctcaaatacc aacaaaacta gcaccagcag tactgaaagc
    13021 aagacaaaag aaaataaaaa atcatcggat gagtagtatt agagggggaa tcataatgga
    13081 acgagcaata tttgcaggag gctgcttctg gtgtatgatc cagcctttcg acacgcttcc
    13141 tggtatccat acagtgatgt caggatatac tggagggcat gtaccagatc cgacttatga
    13201 acaagtaaaa agccaaacga ctggacatac agaagcagtg gagattatct atgatccaac
    13261 attgatttca tatgaacaat tagtagaact ttactggcag caaactgatc caactgacgc
    13321 tttcggacaa ttcgaggatc gcggtgacaa ttatcgcccg gtgatctttt atttgacaga
    13381 agaacagcgg atcattgctg aaaaaagtaa gaaacagtta gctgaaagtg gacgtttcga
    13441 acagccaatc gttacaaaaa tcgaaccagc ccaaccattt tatctagcag aagaagacca
    13501 tcaagagtat tataaaaagc atcctgaaaa ttttaaaaga aaccatgcac gccgggctgc
    13561 ctttatcgaa gaaaattgga ggaacgaagg gtgaatcgaa gcttttttca ttatttaatg
    13621 acactgcgag gcggtggcac acatgatgct ttatctgttt tcgctacaga agccggtaaa
    13681 gacatccaat ttcctaagca cagctctagc tacgaagaaa tttctaatta tctggaactg
    13741 aatgtcgact atttgccgtc catggatatc tttgacgaag catgggaaaa atatttagaa
    13801 aataatcatg acatataaga gatagttttg tactttatga tcaaaaaagt tttttgtcca
    13861 atagcctaat tgaaagaatt atttttattt cagctatcta gtcgcatttt tttgatattt
    13921 ttcttataat aaagaaagaa tgaaaaaaag agggatggat tgatagagat tgacaaaaaa
    13981 acaacgctat attaatcgct tctggcttgt attcaaatac ttacttgttt ctgtattcct
    14041 tgcgttcatg ttgcggggat ttttattcat cccggtacca gtggaaggca attcaatgga
    14101 aaacgtgttg aagcaaggtg atatggttgt gatggaaaaa ttttcggaga tcagacgatt
    14161 tgatattgtt gtttttcaat tagcagatgg cacgatttat atcaaacgag taatcggtct
    14221 tccaggagaa aatgtcagct accaaaatga tcaattgaag atcaatggga aagtagttaa
    14281 ggaaccttat ctaactaaaa acctgaaatc agatcatgca aatgcatcct atacgacgga
    14341 ctttacacta caagagctaa cgggacaatc caagcttccg gaagataatt attttgtact
    14401 tggagataat cgaagagttt caaaagatag tcgttcgttt ggcacgatca ataaaactga
    14461 tattctaggt aaagcaagat ttgtctatta tccacttgat gaaataaaat ggatccagtg
    14521 agaaacaata aagcgtgcag tctgttgaga ctgcacgctt tattgtttaa tccaaaatca
    14581 gtttactacc ttagtacgta aaatcaaaca gctcgtttaa acacttttct agcctctaaa
    14641 gttttgataa atgaataaac aaccattatt tgaataggga tttggatcat attcctgatg
    14701 aatctaccta gaaaaattac tttgaaatct gcaccaaaca aaacagacat cagccaagaa
    14761 ttgataaaaa ggctgactgg gatcataatc aataattggg cagcgatgac ggaaggcagg
    14821 tttatctttt tatgatataa aaataagcca ttaataaatc caccaaggaa agccgtgata
    14881 agaaaagcag cttgaaacgt tcctgaacca aatatcgtga aatttaaagt gttcgacaac
    14941 atttcaagca aaccagccca gatcggtcca tatagataac ccataagcgc catacttaaa
    15001 aatccgaatg tgatttgaaa ataagcagga ccaatcgcta tcttgtataa gatgacatca
    15061 agggctaata aaagagcaga ggtcgacaac aatcttgcat tcatttcggg caatctaaaa
    15121 acgtcttttg caatcaaagt gttcaaaata ttctctcctt tgtcagttaa taataaaagg
    15181 ataccataaa atcaccaatg tgtcatgaca cattggtgat ttacatacac tggcatctaa
    15241 caattaaaaa aagggtgaga agactgcttt tataaactgt aaaaaagaga ttgtacgaat
    15301 aaaaaaaata gaggcggaga atacttatcg tatcatccgc ctttgttttt ttgttataaa
    15361 aaacgtcaga ttagttatct gcttacatct tcatccatac gggttctaat gtatttccgt
    15421 ctagatcttg gacttctaat gaaaacattt cttcttcagg cattcccata tctacttcgt
    15481 ggaaagtacc accattttct tttgcaattt gagcaaattt ttttacttca tccgcatttt
    15541 caacactaaa cgaaatcaaa gcaccacttg tttttttatt atcagctatt tctttatctt
    15601 ttataaattt tttataaaaa tcatgagaga gaagcattat ccaaaaagtg tcatcccata
    15661 ccattgaacc agcttcttca gtagaaaatt cttcattttt tttaaaaccc agcttttcat
    15721 aaaaagcaac ggaacgattg acatcagcaa caggaaaatt aacaaaaacc atactattca
    15781 tttgtttcgt ctcctttctg ttttgttcgt ctatcatctc ttatttagga aacgctgtca
    15841 aaaatttaga ttcatatata gaaaccaaaa taaaaatttg catcatcaaa aaagtgaaga
    15901 tgtgatagaa tgaggactgg ttcactttca cttaactaat agatggaatt tctttataat
    15961 aaaaaagtga atttatattg aaaaagaaaa aaacaagtaa agccggggaa tattccggta
    16021 tgaaaggaat ggagagaaca atgaccatcc aatggtttcc gggacatatg gcaaaagccc
    16081 gacgagaagt ttctgaaaaa atgaaatatg tggacatcgt atttgaattg gtagacgcac
    16141 gattaccttt atcttctcgt aatccaatgc tagatcagat cattcagcaa aaacctcgcc
    16201 ttgttttatt gaataaagcg gatctggcag atccacaaca gacaaagctg tggcaagaat
    16261 attttcgtaa acaaggccat cttgcattag ctatcacggc acaagaaagt aaaggaatta
    16321 aaggtctagt acctgcggct aaagaagcat tgaaagaaaa acgggcaaga gatgctgctc
    16381 gggggatcaa gcctcgcgct attcgggcaa tggtcttagg tattccaaat gttgggaaat
    16441 ctacgttgat gaatcgacta gtaggcaaga aaattgcaca gacaggaaat aagcctggcg
    16501 taacaaaagg tcagcagtgg cttcgtttag gtaatgagtt ggaattgtta gacacaccag
    16561 ggattttatg gcccaaattt gaagatgaag aaatcggcaa aaaactagcg ctgacaggcg
    16621 cgatcaaaga ccagcttgtc catttagatg atttagcgat ttacggatta gacttttttg
    16681 cccgatatta ttcaggaaga atcagcgatc gttatggttt atcaatcgag caagaagaaa
    16741 aattggcacc ggaattactg atggatatta cagcaaaacg tgggtataaa gaagactatg
    16801 atcgtggcag cgaattagtc gtgcatgata ttcgtcaagg aaaacttgga cgatacacac
    16861 tggatcgttg cgaagaaatg gagcaaaaaa gtgatgactg aatccatcca aagtatcaaa
    16921 gaacgattga aaagcataca gagaatagat gatcctacac tagaatcatt aagaaatgat
    16981 ccacgcaaag gcgtgcagca agcactggct tcttttgaac gacgattgaa aaaggaagca
    17041 ttaattaagg aaaaatatga agaaatgttt tgctttgaaa agcaagggta cgcacaaggt
    17101 caccaatgga ttgccggcat tgatgaagta ggaagaggac cattagctgg acctgtcgtg
    17161 gcagctgcag ttatcttgcc gaaaaatgag atcatattag ggctgaatga ctccaaacaa
    17221 ttatcagaga aaaagcgaga acttctttat caacaagttc aagaaaaagc cctggcaatc
    17281 gggatcggga tcgttgatca gcagacaatc gatgatgtga atatttatga agcaagtaaa
    17341 ttagcgatga tccaagcagt agaaaaattg tccattgtac caagctattt gttgatcgat
    17401 gcgatgacct tggatttgcc tattcagcag gaaaaaataa tcaaagggga tgcacgatct
    17461 gtgtcgattg ctgctgcaag tatcattgct aaagtatatc gagaccaatt gatgaaagaa
    17521 tatgacgtgc tttatcctgg atatggtttc tcgcataatg caggttatgg aacaaaggaa
    17581 cacttagaag gacttgaacg acaaggaatt acgccaatcc atcgtaaaag ttttgcacct
    17641 gtaaaaaaat atttacaata aaaaacagcc gcttttacgt atcttattag aaagagagac
    17701 gaaaggcggt tttatgatgt atcaaataga agaaaattta ttgaaacttg cttatacaaa
    17761 aggaatcagt agtcaaggaa aatgggcact tgctaactgg atgatgcagt atccttataa
    17821 agaaatcgga tttgaagaca tcattaagat aggtaaaata accaaacacc gggagctgtt
    17881 tcgcaaaagt tggcaggaaa ttcacgctaa ctggggcaaa atccaatcga aacagtcgtt
    17941 tatcacttgc tttgatccca aatatccccc tcagctgctt cacttgacct atccgccaat
    18001 tgtactgttt tataatggtg atctatcttt actttcttgt aacatgttaa gcgttgttgg
    18061 tggaagacac acttctgctt tagccaaaaa aacagtttat tatttactaa aaccagtgat
    18121 agaagctgga tatgtgatcg tcagtggctg cgcgaaaggg atagatacat acggtcacca
    18181 agcagcaatc tctcatggtg gacggacaat cgcagtcatt ggaacaggga tagagcaagt
    18241 ctatcctgct gaaaatcgtg acttgcaaga ggtgattgca aatgatcatc ttcttttgag
    18301 tgaatatcct cctcaatccg gcccgaaaaa gcattgtttt ccaatgagaa atcggattat
    18361 cgcagctttg tctaaaggaa catgtgtctt tgaagccaag aaaaaaagcg gctctctgat
    18421 aacagcacag caagcattgg atcttggaag aacagttttt tctgttccag gaaatatttt
    18481 aacagaacat tctaccggat gtcatcattt gatccaagac ggtgcagttt gcacggtttc
    18541 gggtgaagat atcttggcag aatttaaaga ataacaccat acttttcatt tgcttttctt
    18601 gacaaacgtt ttctgaatgg atatgattag ctacgatttg tatgaatcct cttttaatag
    18661 taggaacacg acaatggaaa ggagtccatt cagtaatggc gtataaatat ttagtgattg
    18721 tagaatcacc tgcaaaagcc aagacaattg agaaatatct tggacgaaac tataaagtaa
    18781 tggccagtgt tggccatata agagatttac cgaaaagtaa aatgggtatc gattttgaaa
    18841 acaattatga accccattat atttctatac gcggaaaagg cgatgtcatc aaaagcctga
    18901 aagccgcagc aaaaaaagct caaaaagttt acttggcaag tgacccggat agagaaggag
    18961 aagcgattgc ttggcattta gcgtacctac ttgggttgga tctgaaagaa aaaaatcggg
    19021 tggtcttcaa tgaaatcaca aaagacgcag tcaaagcagc ttttaaggaa ccaagaacga
    19081 tcgatgtaga tttagtagat gcacagcaag ctcgtcgtac cttagacaga atcgttggtt
    19141 attcgatcag tcctattctc tggcgtaagg tcaagaaagg gttaagtgca ggacgtgtcc
    19201 aatctgtcgc tttaaaaatt attattgacc gtgaaaaaga gatccgagaa tttgttccag
    19261 aagaatattg gagcatcgac ggtaatttta aaaaagctcg caagaaattc aaagcaaatt
    19321 tctggggaat cgacggtaag aaaaagaaat taccagatgc acaaagtgta aaagaagtca
    19381 ctgctagaat tgatggaaca gacttcgaag ttaaaaaagt agaaaagaaa gagcgcaaaa
    19441 gaaaccctgc gaatcctttc actacaagta gcctgcaaca agaagctgcc cgtaagttaa
    19501 actttcgaac aagaaaaacc atgatggtag cacagcaatt atatgaagga atcactttgg
    19561 gtaaacaagg aacagtcggc ttgattacat acatgcgtac tgattctacc cggattgccg
    19621 atacagcaaa agcagaggcc gcagcattta ttgaagatac gtatggaaaa gaattttcta
    19681 gacatgatca ccgaaagatc aaaaatgctc aaggcgcaca agatgcccat gaagctgtgc
    19741 gtccaacgag tgtgcttcgt acaccagatg aactcaaaca atatttagat aaagatcaat
    19801 tgaaacttta tacattgatc tggtcacgtt ttgtggctag tcagatgaca ccagctattt
    19861 tagatacaat gaaagtgaca ttagaacaaa atggtgtcat cttcattgcg aacggttcaa
    19921 aaatcaaatt caaaggattc atgcaagtgt atgtggaagg tagagatgac ggcaaagaag
    19981 agaaagaaaa tattcttcca gaacttgaag aaggcgatgt agtccaatct gttgatattg
    20041 aaccaaaaca acattttact caaccaccag cacgttttag tgaggctacg ttgattcgtg
    20101 cactagaaga aaacggggta gggcgacctt ccacttatgc accaacactt gatacgatcc
    20161 aaagacgcta ctatgtaaaa ttgacacaaa aaagatttga gccgactgaa ctaggagaaa
    20221 ttgtcaattc attaatctgt gaattcttcc ctcaaatcgt cgatatccat ttcacagcag
    20281 aaatggaagg agatttggat aaaattgaag aaggcacgga agcgtgggtc aaagtggttg
    20341 atcgtttcta taaaccgttt gaaaaagaac tgacgaatgc ggaagaaaaa atcgaaaaaa
    20401 ttcaaatcaa agatgaacca gcaggattcg attgtgatgt ctgtggacat ccgatggtga
    20461 taaaattagg gaaatatggg aagttttatg cttgcagtaa ttttcctgat tgtcggaata
    20521 cgaaaccaat cgtaaaagaa atcggtgtga cttgcccagt atgtcatgaa ggacaagtca
    20581 ttgaacgaaa atctaagaaa aacagactat tctatggttg ttcccgtttc ccagaatgtg
    20641 aattcacttc ttgggataaa ccagtgggca gaaattgtcc aaaatgtgac ggatatctgg
    20701 tcgagaagaa agtcaaaggc ggtaaacaag tagtctgtgt aaatggagat tacgaagaag
    20761 acatccaaaa ataatagaaa agaggttctt tcattgaaaa tggtaaatgt tgttggagca
    20821 gggcttgccg gaagtgaagc tgcttggcag atcgcacaag caggagtccc tgtccgttta
    20881 tatgaaatgc gtccagtcaa atcgactgag gcgcatcaca caagtaattt tgctgaacta
    20941 gtatgttcca attcattaag aggaaacagt ttggctaatg cagtagggtt attgaaagaa
    21001 gaaatgcgcc gattgaattc ggtagtaatc acttcggcag atgaaacagc agtgccagct
    21061 ggtggtgcct tagctgttga ccgcgattct ttttctgaaa caattacccg aaaagtcaaa
    21121 gagcatccat tagtaacagt gataaacgaa gaaattactg aaataccgga aggaatcacc
    21181 gtagtagcaa caggaccttt gacttctcat ccattagcag aaagcatcaa agcattcaat
    21241 ggttcagatg gtttttattt ctatgatgca gctgcaccaa tcgtagacaa agctacgatc
    21301 gatatggata aagtatattt gaagtcccgc tatgacaaag gagaagcagc ttacttgaac
    21361 tgtccgatga cagaagagga attcaaagct ttccatgagg cactaatcaa tgccgaagtg
    21421 gcagaactta aatcttttga gaaagaaaaa tattttgaag gatgtatgcc aattgaagta
    21481 atggcacagc gaggattcaa aacgatgctt ttcggaccta tgaaaccagt tggattggaa
    21541 gatcctaaaa ctggcaaacg tccttatgct gttatccagc tgcgtcaaga caatgctgca
    21601 gcttctttat acaacatcgt gggctttcag actcacttga aatggggcga gcaaaaacgt
    21661 gttttccgga tgattcctgg gttagaaaat gctgaattcg ttcgctatgg tgtgatgcac
    21721 cgaaacagct ttatgaattc gccagaattg ctgaaaccga cttaccaatc aacgaaacgc
    21781 gaagatctgt tctttgcagg tcagatgact ggtgtagaag gctatgttga aagtgcagcg
    21841 agcggactac ttgctggaat caacgccgca cgcttagcaa aagaaatgga accagtcgag
    21901 tttccacaag aaacagctat tggaagtatg gcttactaca ttactcatgc ggaaggaaaa
    21961 catttccaac cgatgaatgc caactatggt ctgtttcctg aactgccaga gcgcattcga
    22021 gacaaaaaag caagatatga agcaattgca caacgagctt taaaagctaa tgaggcaata
    22081 aaaacagaat tagaaaaaac tgcaatccca aatgagtaag gaaactccat gaagagggtg
    22141 agacggatag tctcgctctt tttcttgcgt ttttcttaag aatttctcaa acaaaatgtg
    22201 aggaattgcg ccaattgtga acaaacttag aaatattggt taaattctga caatttattt
    22261 gtctttttat aaacgctgtg ctaaattatt ctctgggaca aaaggaaggt gagaaaatga
    22321 cagaagccac gtgggaacaa aaatttttgc gttatttgat tgtcgaaaga ggatattcag
    22381 aaaaaacgaa agaagcatat gaagaagaca ttctccattt caaacatttt ttggaggaat
    22441 ctggagacgc tgatctgctg aaggcggaat attttacggt tcgtgtctat ttgagtgcat
    22501 tagctgatca tcgtcctagc tatagcagaa attctatcag tcgcaagata gccagcctac
    22561 gttcgtttta tcagtatctt ctcaaagaag aagtgataaa agaaaatcca ttttcttacg
    22621 ttcatctcaa aaagaaaaat ttgagactgc cacgtttttt ttatgaaaat gaaatgcaag
    22681 cactttttga cagcgtagca ggagatagtc ctttggattt gaggaaccgc gctttactgg
    22741 aagtccttta cgggtcaggc atccgtttaa gtgagtgtag cagtttgtcg gtatcagaca
    22801 ttgattttga ttcagaggtc atgttgatcc atggtaaagg gaacaaagag agatatgctc
    22861 ctctaggttc ttttgctcaa gatgctctgc aagaatattt cagggaaggc cgccaggtat
    22921 tgatgacaaa atatcacgaa gagcatgata tcgtttttgt gaatcatcat ggaaaaccaa
    22981 tcacgcctac tggaattgaa tatgtcttga atcaaatgat caaaaaaagc agtttggata
    23041 gtgatatcca tcctcacatg ctgcgccata catttgctac tcatttatta aataatgggg
    23101 cagatttgcg gacagtccaa gaattgctag gacatgccaa tttatcgact acccagatct
    23161 atgcccatgt aacaaaagaa agtttacaaa aaaactatcg ttctttccat ccaagagcgt
    23221 aaaaataagg aaaagaacaa atttaggagg aaacaaacat ggtcgaatca ccatttcatt
    23281 caaccactat ctgtgccgtc gaaaaagacg ggaaatttgc tatggctgga gatggccaag
    23341 taacaatggg tgaatctgtt gtcatgaaag gcactgcaaa gaaagtacga cgtatctaca
    23401 atggagaagt tgttgtaggt ttcgccggaa gtgttgcaga tgcgtttact ttagaagaaa
    23461 aatttgaagg caaactaaac gaatataacg ggaatctaca acgcgcagct gtcgaattag
    23521 ctcaagaatg gcggacacag caatctatgc aaaaacttga agcgatgctg atcgtaatga
    23581 atgataaaga aatgcttctt gtttcaggta caggagaagt tatcacacca gatgatggta
    23641 tcttagctat cggatcagga gggaattttg cgttggctgc agcccgagca atgaaaaact
    23701 atgctcaaaa cgacatgagt gcgaaagaaa ttgcagaaaa tgcgttaaat attgccgcag
    23761 atatctgtat ctttactaat cataatatta tcgtagaaga actgtaagag gtgagtagat
    23821 atgtatgaat taaaacaaac accaagacaa atcgtaagtg aattagatga atatatcgta
    23881 ggacagcacg cagcaaaaaa atctgttgcc gttgctttgc gtaaccgcta tcgccgtttg
    23941 cagttagatg aaaaaatgca acaagatatt acaccaaaaa atatgctgat gatcggacct
    24001 acaggggtcg gtaaaacaga aatcgcacga cgtttggcaa aaatcgtcaa tgcaccattt
    24061 gtcaaagtcg aagcaacaaa atttactgaa gtcggttatg tcggaagaga tgtagaatca
    24121 atggttcggg atttagtaga aaatgcgatc caaatcgtag aaaaagagca gtacagccgt
    24181 gtatacacaa gtgctaaaaa aaatgcagaa aaacgtttgg taaaaatctt ggtacctggt
    24241 atcaaaaaag aacagaaaca atccactaat caatttgaac aaatgatgaa tctttttaat
    24301 gctagtcagc aaaataccgc tgaaccgcaa gaagaagtga cagaagatat tcgaatcaat
    24361 cgccgtacag tcttggaaca actacaaaag ggtttactgg atagtcgtga agtaaccatt
    24421 gaagttgaag accaaaagaa acctgcacca atgatgaaca atggattgga gcaaatgggg
    24481 atcgacctaa atgaaactct aggtgctttg acaccgaaga aaaaagtgga acggacggta
    24541 acagtcaaag aagctttaga gttattgatc aaggaagaat cagcaaaatt agtcaaggat
    24601 gctgatatcc atagtgaggc aatcaaatta gcggaagaaa gtggaattat ttttatcgat
    24661 gaaatcgata agatcacttc taaatcacag caatctggag aagtatccag agaaggcgtc
    24721 caaagagaca tattgccaat tgtcgaaggt tcacaagtaa acacaaaata cgggatcatc
    24781 caaacagatc acgtcctatt cattgcttca ggagctttcc atttgtctaa accaagtgat
    24841 ctaattccag aacttcaagg acgcttcccg atacgggtag aattagatga tttgacagct
    24901 gaagattttg tgaagattct gaccgaacca aataatgctt tagtaaaaca atacgtcgcg
    24961 ttgatcggta cagaaaatgt gtcagttacg tttacaaaag aagcaattga acggatcgct
    25021 caaatcgctt tcgatgtcaa ccgtgacaca gataatatcg gcgcacgcag actgcatacc
    25081 attttggaaa aactactaga agatctatta tttgaagcac cagatatgca aatgggagaa
    25141 ataacgatca cagaagcata tgtggatgac aaacttggct caattgcggc aaatgaagat
    25201 ttaagtcgct atattttata aaaaatcgga ggacgacaat gacagacttg ctaacgaaaa
    25261 cccgcaagat aaacacactt ttgcagcaaa agaatacttt tgacatgcaa gcagatttgc
    25321 cttacgataa aatggctgtc acactaggtg gtattcttga cagcaacacc tatatcatag
    25381 gaagtgacgg agtattacta ggttataatg aacgtcacga tgtgaacaat gaacgaatca
    25441 aaacaatgtt tgttgagaaa caatttcctg aatcttatac tgatacagtg gcacagttga
    25501 tgaaaactga agctaatatc ccaattacta gtgatattac tgcatttccg gtagagtcaa
    25561 gagagaaata tccatttggt ctgacgactg tcgttccgat ctttggagct ggcgaacgtt
    25621 tgggtacgat tatcttatct cgtatggaac aatcgtttgc agatgacgat ctagtcttag
    25681 cagaatatgg agctacggtt gtcggtatgc agatccttta tcagaagtct cgaagcatcg
    25741 aagcagatgt acggagtgca acggctgtcc aaatggcaat caacacatta tcttatagcg
    25801 aattgaaggc tgtacaagct atctttgaag cattggatgg agatgaaggt cgtctgactg
    25861 cttctaatat tgccgatagt atcggcatca ctcgttctgt gatcgtcaat gctttacgaa
    25921 aattggaatc agctggaatc attgaatctc gttcgttagg catgaaggga acttatttaa
    25981 aagtattgaa ttcccgtttt aaagacgagt tagcaaaaca cagttattag aagtaagggg
    26041 actggggcag atagaaatgt ttgttccagc cttcccattt taaaaagtaa gtcgaagttg
    26101 gaggaatggc aatgactgtt attattcaaa atgaccagct gatcgccgaa atcgctgaac
    26161 acggtgcgga attgatcagt ctgaaatcaa aagaacatgg tttagagtat atctggcaag
    26221 gtgatccagc gtattggggc agacatgcac cggttttatt cccttttgtt ggacgtttaa
    26281 aagatgatca gtatgtttat caagggaaaa catatcaaat ggggcaacac ggatttgctc
    26341 gggatatgga ttttgaagtc attgaacatg gacaagaaat ggctagtttc atcttaagga
    26401 gtacgccgga gacattagaa aagtatccat ttgattttga attagtgatc agctatgaat
    26461 taggaggaga cggcatcacg gttcattatc aagtggcaaa taccggtgaa aaagaaatgt
    26521 tcttttctat cggtggacat cctgctttca atgtacctct tgaagaagga ttgtcatttg
    26581 aagatttcta tctggcattt tctccattga aatcaagatt acgcctacca ttgaaaggcc
    26641 cattcatcga tatggatcaa aagacgttag gtcagacaaa tacgaatctg gctttgaatc
    26701 gtgaactttt tgagcaagat gccttggttt ttgaaacaag gggtctaaat gcgtacgcca
    26761 ttcgaagtga aaaatcttcc catagcgtaa ctttaagcta taaagatatg ccttatgtag
    26821 ggatctggtc tccttatcca aaagaagcgc ctttcgtttg tatcgaaccg tggtgcggaa
    26881 tcgctgacac ggtgaacagt agtggaaact tgatggaaaa agtagggatc aatcaactag
    26941 gtgcccatga aatcttcaag acaaaatatt ccatcacagt gaagtaagag gttgtgaccg
    27001 agaagttagc agggaacacc gtttaccaag gtgtgtaaaa aagctatcct gtaagtaata
    27061 agggagtgtg acatcgattt gtcacactcc cgttttatct ctttggaatg aaaaagtatc
    27121 aggatttttt cttgctgcta ttcaaaccaa acgggattcg actttcagta tggtttctga
    27181 tacgcttgat attgtcttta tgccgataaa agataaagga agaaactaaa aaggcaatga
    27241 tcgtcagtaa ccaattgggc ttagccaaaa tcacaggagc tacataagga aggacgatcg
    27301 tagataaagt gatcaatact gcactgatca tactgctcaa actcaccata cttgtacaaa
    27361 ataaactaac gataaagatg atacaagaat aaacaaaaaa tacaggatta aatcctaaaa
    27421 gcattcctgc acttgtcgca acagcttttc ctcctttgaa cccagcaaaa atcggaaatg
    27481 tatggccaag aatggcgcat accccaaacc aaagagggtt aatcccagaa aggtggaaga
    27541 tcatcggcaa acaagtagcc aatgtacctt ttaaaatatc cataagcagg acaacaatcc
    27601 cggctgtttt gcctaataca cgaaatgtat tggtcgttcc agtattaccg ctcccgtgtt
    27661 gtcggatatc ttttttgaaa aacaatttgc caatccagac accggaagga atagaaccta
    27721 aaagatatgc taaaataagt aataagatca atttcatttt ttaccctctt tcaagttgaa
    27781 aactatcctt tattttagca tgaataggta acaggaacaa ttgaaaaaat gaaagataca
    27841 agtataatag atggaataaa aagaaaagca aggggtggca gaaatgtctg taaaaaatcc
    27901 aacacaagaa acgattttca actatttgga agaagcaaaa aatattgccg tcgtaggctt
    27961 gagcaataaa ccagaacgaa caagttacat ggttgcacag gtattgcaag acaatggata
    28021 taaaatcata ccagtaaatc cattattggc tggtgaagaa attctaggcg agaaagtcta
    28081 tgcgaatttg acagaaatcg atgatccaat cgatatcgta gatgtattta gacggagtga
    28141 atttttacct gaaatagcag gtgatttttt gaaaactgat gctaaagtgt tctgggcaca
    28201 attagggatt gaaaacaaag aagcagctga gatgctaaga aaagcaggac gtacagatat
    28261 cgtaatggat cgttgtatta agattgaact tggtaagttg aaagaaaacc aaaaataaaa
    28321 atgaccacaa agaagagatg atcgatagag gggaatgtcg aaaaagcggt taggagataa
    28381 ttaaaattga tttctcgtct accctctttt ttttcacgac cttttctagt atgataatcg
    28441 tggtgcggtt ttgccgcaaa gtattattga aggagcgttt gccttggcaa aaaaagtaaa
    28501 caacgaatac aatgactcgt ccatccaagt attggaagga ttagaggcag ttagaaaaag
    28561 accagggatg tacattggat ccactgacag ccgaggcttg catcatttag tctatgaaat
    28621 cgtcgataat gcagttgacg aagcattatc aggttatggc agtgagattg atgtgaccat
    28681 ccatgaagac aatagtataa ctgttgcaga tagcggacgt gggatgcctg taggaatgca
    28741 tgcttccgga ataccaacag ttgaagtgat ttttactgtg ctgcatgctg gcggaaagtt
    28801 tggtcaagga gggtacaaaa cttctggtgg actgcacggt gtaggagcca gtgtggtaaa
    28861 tgccttatcg aaatggctaa ttgtaacgat cgttagagat ggcgtagagt atcagcagaa
    28921 atttaaaaac ggcggtaaac ctgacggtac tttgaaaaag ataggaaaaa caaaaaaagc
    28981 aaacgggacg actgtccatt ttttaccaga tgatacaata ttcagtacaa cgaaattttc
    29041 atatgaaatt ttagccgaac ggttaagaga atctgccttt cttctaaaag gcgttaaaat
    29101 ctctctttct gatcttcgtg gagaagaacc agtcaaggaa atttttcatt acgaagaagg
    29161 aatcaaagaa tttgttgatt atctgaatga agaaaaagat acattgacac cagttgttta
    29221 tttttcagga gaaaaagaag gtatcgaagt agaggtcgct tatcaataca atgacggtta
    29281 ttcagaaaat gttttatctt ttgtcaataa cgttcggaca aaagatgggg gaacccatga
    29341 agctggtatg aaagcggcga tgacgaaatc ttataatgaa tatgcgagaa aagttggttt
    29401 gctgaaagaa cgggacaaga atctggaagg tagtgatttc agagaagggt tagctgctgt
    29461 actttctatc cgtgtacctg aaaatcttct gcaatttgaa gggcaaacaa aagaaaaact
    29521 tggtacgcca gttgctcgta ccgtagtaga taacgtcatc agtgaacaaa tgggctttta
    29581 tttgcaggaa aatagcgaaa tgagccagat gttggtccgt aaagcaatca aagccagaga
    29641 agcgagagaa gcagcacgta aagccagaga agaaagccga aatgggaaga agcgtaaaaa
    29701 aggcgaatct cttctttcag gaaaattgac acccgcacaa tcccgaaacc ctaagaaaaa
    29761 tgaattgtat ttagtcgaag gggattctgc tggcggatct gctaaacaag gaagagacag
    29821 aaagttccaa gcaattctac ctttacgagg aaaagtcatc aataccgaaa aagctaaaat
    29881 gcaagatatc ttgaaaaatg aagaaatcaa tacgatgatc tatacgattg gtgcaggagt
    29941 cggtcctgag ttttctatcg aagactgtaa ttacgacaaa gtaatcatca tgaccgatgc
    30001 ggatacagac ggcgcccata ttcaagtatt actgcttact ttcttttatc gttatatgaa
    30061 accgctgatt gaagctggga aagtatatat cgcgttaccg cctttatata aagtatccaa
    30121 aggacaagga aagaagcaag tcattgaata tgcgtggaca gatgacgaat tggcagcaat
    30181 gattaaaaaa gtcggcaaag gatatatgct gcagcgatat aaaggacttg gagagatgaa
    30241 tgcggaacaa ttgtgggaaa caacgatgga tccgacttcc cgtacgttga tccgcgtccg
    30301 tatcgatgat gctgcgcaag ctgaacgtcg agtaaccaca ctgatgggtg acaaagtaga
    30361 acctcgaaga aaatggatcg aaaatcacgt gcaatttacg ctcgaagaag atggcagtat
    30421 cttagataaa aaagaggata ctgaaatctc tccttcagtt tctaatgatt tattagatga
    30481 agaacgagca gataaaaatg aaaacaatca gttatttgaa gtagaatagg aggtcacctg
    30541 atggaaaatc gacaagaagt acaagaatta actttagaag aagtgatggg tgaccgcttc
    30601 ggacgttatt ccaaatatat tatccaagaa cgtgcactgc ctgatatccg tgatggattg
    30661 aagccagtgc aacgccggat attatttgcc atgaacaaag atggcaatac gtatgacaaa
    30721 ggattccgta aatcagcaaa atctgtcgga aatatcatgg ggaattatca tcctcatggt
    30781 gacatcagta tttatgaagc gatggtccgc atgagccaag actggaagtt gcgggaagta
    30841 ctgatcgaaa tgcacgggaa taacgggagt atggacggtg atccacctgc tgcgatgcgt
    30901 tatactgaag ccagattatc caagttaagt ggagagatgc tagcagatat cgagaaagaa
    30961 acagtcgatc tcgtgtggaa ttttgatgat acagaaaaag aaccaacagt ccttccagct
    31021 cgatacccaa accttctcgt caatggatcg acaggtatat ctgctggata tgcaacagaa
    31081 atcccgactc ataacttagc tgaagtgatc gatggcacaa tctatatgat cgatcatcca
    31141 caagcgagcc ttgaaaaatt gatggaatat attcctggac ctgactttcc aacaggaggg
    31201 atcttgcaag gaaaagatga aatcaaaaaa gcttatgaaa caggacgagg aaaagttatt
    31261 cttcgttcga aaacaaagat agaggcaatg aagggtaata aacagcaaat cgtgatcaca
    31321 gaaatcccat atgaagtaaa taaagcgacc ttggtgaaaa aaatggatga gatccgtttg
    31381 aataagaaaa tcgatggaat agctgaagta cgagatgaaa gtgatagaac aggtctgcaa
    31441 attgtcgtcg aattgaaaaa agacgccaat gctcaaggaa ttttgaatta tttgttcaaa
    31501 aacactgagc tacagatcaa ttacaatttc aatatggtcg ctatcgacca tatgactccg
    31561 catcaagttg gactgaaaga cattttaaga agttatatcg agcatcgaaa gcaagtcata
    31621 acaaaacgta gtcaattcga tttagccaaa gctcaaaaaa gacagcatat cgtagaagga
    31681 ttgatgaaag ctttatcgat tctggatgaa gtaatcgcaa cgattcgtga aagtaaagac
    31741 aaaaaagacg ccaaaaagaa tttagtcgac gtctttcaat ttacagaaga acaagcagaa
    31801 gcaattgtca cattgcaatt ataccgtttg acgaacacgg atatcacaga gcttcaaaag
    31861 gaatccgaaa gcttgattgc gcaaatcaca gagttgaata aaatcctttc aaatgacaaa
    31921 gagcttttct ctgttatgaa aaaagaattg cgagaagtga aaaaaaatta ttcttccgct
    31981 cgtctaacga caatcgaaga cgaaatcgaa gaaatcaaaa ttgatacgca agtcttagtg
    32041 gcacaagaag acgtaattgt cagtgtgacg cgagaaggct atgtgaagcg aacaagcctt
    32101 cgttcttata gtgcttccaa accagaagaa atcgggatgc gagaaggcga ctacttgcta
    32161 tactcaggtg aattgagtac attggaccat gttcttctga tcacaaataa agcaaatgtg
    32221 atctaccgcc ctgtccatga actacccgat ttgaaatgga aagatgctgg tgagcacatt
    32281 tctcaaacaa tctccaatct gtctgtagac gaatcgattt tagctgtttt cccatatcaa
    32341 aaaattcaag cagagaaaac atttgtattc atcagcaaga atggattgat caagcaaaca
    32401 cgaatgacag agttcgaacc atggcgcacc tataagagcc gtccgctcag ctgtatgaag
    32461 ctaaaggcgg aagaagatga actgattgct gtgtatctga aagaagatca gccaggctta
    32521 gatgttttct tagtaacgca tcaaggaatg ggtttgcgct atcctttaac agatgttccc
    32581 gttgttggag caaaggcagc tggagtgaag tctatgaatt tgaaagatgg agataggatt
    32641 atcaacgggc tgcttgtgtt agcagaaggt gatacgccga tcgtaatcgt tactcagcga
    32701 ggagctgtta aacgcatgct tgctcaagaa atcagccaaa cttcacgtgc gaagcgggga
    32761 gtgacggtca tgcgtgagct gaaaaaaaat ccgcaccgta ttatctatat gtcggaagga
    32821 agatcaaaag aaataacctt gatgaatcaa aaagggcagc agttaacgat tgacccaact
    32881 gatttcccta taggtgatcg aacatcgaat ggatcatttg cgatggatga gaaaaaaggt
    32941 ggagaagtca tcgaagttat cgatgctcct gagattttca tcgagtcgta actgttgtag
    33001 tattttaatg aagagacgcc atctgtatta atacaggtgg cgtctctttt tcattcattt
    33061 ttaataaatc cttatacgtt tgcaaaaaaa gatgaaagtg ttcacaatgc ttaaatgttg
    33121 gtatgtcaac gttgatttaa atatatgtaa ataaaacata aaaattgtta aataaaaaac
    33181 aagaaaaccc ttgcaaagat agggtgctgt gttatactaa tcacgtaaaa actgttacat
    33241 aaaacagaaa tttaggaggt ccatacaatg gagcaatgga atggatttaa aggtaccaaa
    33301 tggaaagaag gcgtagacac tagagacttt attcaaaata actacactga atatcgcggt
    33361 gatgacagct ttttggaagg cccaacagaa gcaacagaaa aattatggga tgaattgcaa
    33421 aagttaaatg atatccaatt cgagcgtaac ggtgtctatg atatggacac agaaatcgta
    33481 tcaactatta cttcacacga tgcaggctat ttggataaag atttggaaaa aatcgtcggt
    33541 ctacagacag acaaaccttt gaaacaagca ttcatgccgt tcggcggaat caatatggct
    33601 aacaatgcgc taaaaagcaa tggctataca ccaaacgatg aattgacaca tattttcact
    33661 gactggcgca aaacacataa tcaaggtgta tttgacgctt atactgctga aatgcgtgct
    33721 gctcgtaaaa acaaaatcat tacagggctt ccagacgctt acggtcgcgg acgtatcatc
    33781 ggtgactatc gtcgtgtggc attgtatgga atcgactatt tgatggaaca aaagaaaaaa
    33841 gatcatgata tgactggtta tcatacaatg agtgaagaag tgattcgttt acgtgaagaa
    33901 atcactgaac aatatcgtgc attagcgcaa atgaaagaaa tggctgcgaa atatggctat
    33961 gatatttcta aaccagctgc aaatgcaaaa gaagctgtcc aatggctata cttcggatat
    34021 ttagcagcaa tcaaatcaca aaatggtgct gcgatgtcaa tcggacgtat ctcagcattc
    34081 cttgatatct atatccaacg tgatttggat aatggagtaa tcacagaaaa agaagcacaa
    34141 gaattgatcg accacttagt aatgaaatta cgtatggtca aattcgcgcg tacacctgaa
    34201 tataaccaat tattctcagg attccctatc tgggctactt tatccattgc aggtatggga
    34261 ttagatggtc gttcattagt tacaaagaat gatttccgtc tgcttcatac attaacaaat
    34321 atgggacctt caccagaacc aaacttgact gttttgtatt ctgaacacct tccagaagga
    34381 ttcagaacat atgctagcag aatcgctatc gaaagttctt cgattcaatt cgaaaatgat
    34441 aatcagcttc gtgaacaatg gggttctgat gattgtgcga ttgcttgctg tgtatcagga
    34501 acagttgtcg gaaaagacat gcaattcttt ggcgctcgtg ctaacttggc taaaacacta
    34561 ttatatgcca tcaatggcgg agtcgatgaa gtgactggcg cccaagttgg accagaatat
    34621 cgtccaatca catcagaaat actagacttt gaagaagtga aacataactt catgaatatg
    34681 atggattggt tagcagaact atatgtcaat acgttgaaca tcattcatta tatgcatgat
    34741 aaatattctt atgaagcagc tcagctggct ttgatggatt ctgacttgca acgcacattt
    34801 gctacaggta ttgctgggat ctctcatgcg gcagacagct tgtcagcgat caaatatgca
    34861 aaagtaaaag caatccgtaa tgaaaaagga atagcagttg attttgaagt ggatggcgac
    34921 ttccctaaat acggaaatga tgatgaccgt gcagatgaat tagtttcatg gatcttggaa
    34981 tacttcatgg gacaaatcaa acgtcataaa acttaccgca actcaaaacc aacaacttca
    35041 ttattgacga ttacttcaaa tgttgtttac ggtaaaaata caggaaatac tcctgatggc
    35101 cgtgaagctg gaaaaccact tgcgccagga gcaaatccaa gttacaatgc ggaacaaagt
    35161 gggttattag ctagcctgaa ctcaacagct aaaatgaatt acggctgtgc attggatgga
    35221 atttcaaata cccaaacaat caatccttca ggtcttggaa aagaacaaga tacaagaatc
    35281 gaaaacttgc gtaatgtctt agatggatac ttctcaaaag gagcttatca tttgaatgtc
    35341 aatgtattca gtaatgattt attgcgtgat gcaatggaaa atccaatgaa atatccgaac
    35401 ttgacgatcc gggtatctgg ttatgcagtt aaattccgtg acttgacaag agaacaacaa
    35461 ttagatgtct tgatgagaac ttcacacgac agaatgtaaa aagttcgtga aaaaagtttt
    35521 tcgacttagc gtcgaaagga cgttgaatag ccatcgcatc aaaatcgaat aatcagtatg
    35581 cttatttgaa agaaacgatt tacaggggca ggacttgtat gttccgtccc tgtaaatttt
    35641 acacataacg aagcggcaat tgcgaaaaat tttgttcaga aagcttcgct tcgacagcta
    35701 acttgtactt aaaatgaaat caaagaaaag atgtgaactt aaatggaaga aaaaacaatc
    35761 ggctatgttc attctatcga aactttcggt tcagtagacg gacctggact gcgctttgtc
    35821 gtgtttatgc aaggatgccg catgcgttgc caattttgtc acaatccaga tacttggaat
    35881 attggcggag ggaaagaata cacagcagat gaacttttag ataaagcaga acgcttcaga
    35941 ccttactggg gagataaagg tggtattact gttagtggtg gagaacctct attgcagatc
    36001 gattttttga tcgaactatt taaaaaagca aaagagcgga agatgcatac tacattggat
    36061 acatgcggaa aaccttttac gtatgaagaa cctttctttt cacgttttca agaattgatg
    36121 aagtatacag atttactgtt atttgacatc aaacacatcg ataatgaaga gcataaaaaa
    36181 ctaacgcatt gggacaatga aaatatttta gaaatggctc aatatttgtc aaaaatcaac
    36241 aaaccggttt ggattcgcca tgtactagtt cctgagcgaa gtgattatga tgaatatttg
    36301 atccgactag acaactttat tcaaaccttg tcaaatgtag acagagtaga gattcttcct
    36361 tatcatacga tgggaaaata caagtgggaa acacttggtt tgaaatatcc attagaaggg
    36421 atcgaaccac ctacaaaaga acgtgtcgaa aatgctaaac gattgcttca tacagaagag
    36481 tatacaaaat atttaacgcg ataggtcgca tcggaaagaa gataagtatt gcttctttcc
    36541 gtttttttgc tattattaaa taggagaaat cacaaaggag cgaatgacat gtcaaaaatt
    36601 ttagtttttg gtcaccaaaa tccagataca gatgcaatcg gggcagccat tgcgttttca
    36661 cacttgcaaa atgaattagg taaagaaaca gaagcagttg ctctaggtac acctaatgaa
    36721 gaaacacaat acgcattaga ccactttcag ctatctgcac cacgagtagt agagtcagct
    36781 aaagcagaaa atgcagatgt catgcttgtg gatcataatg aattccaaca aagtatttca
    36841 gatatcagcg aagtaaatat tttagctgta gtagaccacc atcgtattgc aaactttgaa
    36901 acagctaatc cattgtacta tcgtgcagaa ccagttggat gtacaagtac gattatttta
    36961 aaactatata aagaaaacca agtaacaatt cctaaagaaa tcgcaggaat catgctctca
    37021 gctatcattt cagatacatt gttgttcaaa tcaccaacat gtacgcctga agatgaagca
    37081 gctgcaaaag aacttgcagc aatcgctgaa gtcgatctaa acacttatgg acttgaaatg
    37141 ttgaaagcag gaacaaatct aagtgataaa tcagctgcgg ttcttcttga tttggatgca
    37201 aaaagtttcc caatgggcag ttccaacgtt cgtatcgctc aagtcaatac agtggacctt
    37261 aatgaagtga tggatcgaca agcagagcta gaagcagcaa tggaagatga aatgaaagca
    37321 aatggctacg atctgttcgt attagttgtc acaaacatct tagacagtga ttcagaatta
    37381 cttgttgctg gtaaaggaaa agaaaaagta gaagaagctt tccaaacaac tttgaccaac
    37441 aaccgtgctt tgcttaaagg tgtcgtttca cgtaaaaaac aagtcgtacc tcaattgacg
    37501 gctgtattta gctaatcaaa tggtagtatg aagcattcaa aatgaatgga taagcgattg
    37561 atataaaaag aaacatagaa catactaaag gccgaagaaa tggaggagaa taacagtcaa
    37621 ttatgactct tgttcttcct cctttttacg taaaagcctt ttaagtaaaa taaaacggat
    37681 agaaaagaga acatatatga actattattc aaaaaataag gaaaactttt atcaaaaact
    37741 gaccgatgat ccgctttttt cttcattgac cgattatcta tatgaacatc gagaacaaga
    37801 aacgattcta agagaattga aaaaagaatt ttcgcaaaat aaattttccc attttcttga
    37861 tttattgatc gatgcaggat taatcaagag ggaagaacga agatatcatt tgaactttcc
    37921 aatatttgat cctaaagatt acttgcaaca agccacttcg gctgctgaaa ccatagctga
    37981 acaattgaag agactctctg ttgatgaaca aaaattagcg atgggagaag ttatttgggc
    38041 gtattgtttc gaagatgaac ggaaagaagc gtatttttat ggagtaagaa acagccgaga
    38101 gacagaattg ctgaggacaa ctgcaggaaa taagaagtac cgttttatca cactttcctc
    38161 tatagaacac ttcccgctta ctttggctaa ttactttttt gtacagaaga atcagcttcc
    38221 tgtaacgaag gcattcaaag aattagccga acttattgga gacgtaaatg aatcgtactt
    38281 ttttgatcaa atagaagtta tcgttgacag gatacgtaag aataaatata aaaatcgccg
    38341 gcctagtatt ttccatcagt ctctacttgt gacgaatact ataaaggaag aagaatcatt
    38401 cacattagaa ttacctattg tcgaaaaaaa caattttgag attgaattac caacattgga
    38461 tccttcattg acaatggagg aaatggcctt tcttaaacga caaatctttt ctgagctgag
    38521 caagaaattt ataccgcatg cattttctta tatcaaagaa tatggaacag ttttagtgag
    38581 taaaacctaa gagagcagta agcaaaaatt atactgactc ttgcaggagg agcctttctc
    38641 ttcctttatg gttaaaagaa ttatttgagt attttgccta tctaccacct attagattca
    38701 ctacatggtc tggaaacaaa ggaatgcaaa aaaatggtga gaaatgtgga atgacaaagg
    38761 aagtagtgat aagaaaagtt agatttttaa ataatcaata ttatgattca gtcaagtatg
    38821 gcattttatg ggaagaacta gctgattaga ttaggaagtc catttgataa tccagaatca
    38881 ctaagaaacg aatggcgagt cattaataat ttatccaatc ttgggttatt cttttagatt
    38941 gaagtgttta aaactaaaaa agcatttatg aagtcgtaat agtcaggctt caaaatgctt
    39001 tttattttgt ttgttttttc tttgccacat aaatacacct agactgacga tgaagagcat
    39061 atccaacaga tttcctgcga cttggatcgt cgaagggttg gtcagtggca ttaaagcaag
    39121 gacaaaaaag gaaaacaagc tgtaaataaa acaggtgaca ggaactaatc gaaagaagag
    39181 cggacatttc aattggctaa aaatgagtga aaaaataaaa taagtaaaaa tggataaaat
    39241 cacgactaaa ccattcatga taaagaagaa aagcgtattg tgattaaacg aataaaaaag
    39301 aattgctaaa aaagaaaacg ttttaaatgc aatcaatacg cttgaagctt cgtttaacgt
    39361 accaggatgt cgatgaaaaa aataggcacc gaaataaaca gaaaacagta agccgcaaag
    39421 agaagacaaa atcaaaaaaa acggattttc ttcggcttct gtgatatcag aatgatccaa
    39481 aagcaaccag cctaatgcac tagaaggcgc atgtgaggtc ttaaggtatg aataacatag
    39541 aaaaaataag atcgtcaaag ttgcaaaaaa agagcctaaa tgtaaaaaat tagtggttct
    39601 acgttgaatt tccataatat ctccaaatta aattgataag tatataaagt ataataacaa
    39661 attgatgagt atatgtcgat gttttttaat caaaataata aataaaaact gtctaacatt
    39721 taaaatttaa tttagatatt agacagtttt taagtttgtg agttattttc cttgtcgttt
    39781 tctcactttt ttagtccaga aaccacctga aatatatttt ggctcataag aaatgataaa
    39841 agcacgctct tcgatttctt tgatcaggcg atacagttca cgttcgcttt ttctaggaga
    39901 aagtatttcg agaactagcc gctcaccttc cagccccatt gcagaagtct gtgtcacacc
    39961 ataaccgtgt tctctcagta tacccggcaa acttttatct tctgcgctat ttgctggcaa
    40021 aatcgttgtg accatgatgt atcctaatgc caatttgtct tcaatcaaaa ttccgacact
    40081 gatacctact gcgtaaccta acgcatagac aaataaattc agtggattat ctaaacgatc
    40141 taaaacaagg gataagccga cgatataaat cgtgatctcg accatactga caagtggagc
    40201 aatcagtcga tagcctttca ttgtcaacat gaaacgtaac gtgttcagag tgatataagc
    40261 aaaattaatg aggaagatca tccccaacat ttttggatca atcatttgta aaactccttt
    40321 catttatata acaacaatat caaaaaaaat cgtaagtgaa gaaaaattga aactttatac
    40381 atacaaaaaa atgataaaat gattataata gaattataaa gataggttag gtgattaaaa
    40441 tgaaaacggt caatcgtgtg ttttgtgcca gccacgtgct aagtctcctt ctcagtatat
    40501 acttaggtgt ctattcgatt caacagccaa ttgaggtaga gaacattgtt tttcagatgt
    40561 cgctgacaga tggtttaatt ttaagcgttt ttttcttttt gatactgttt attggtaata
    40621 ttttcggtag cgttgccagt tttttgaatt tttctattta tccactcctt tcgctagcat
    40681 taggagtggt gggactttgt tctctgtgct tatttcccga gccattcagt cctgcattta
    40741 tattatttgg tacactgaac ctgttccaga caactgttgg agcatggttg ctatggcgat
    40801 caggaaattt aatgaaaata ggtgagtaac aattgaatcc aattatattg aaacgttctg
    40861 tccatcatat tccagtgtta gaagttgttc tgaaagaatt aagagaggaa cgtattcccg
    40921 ttgtcatcta ttatcacgga tggcagacaa gtaaggagtt agttttgacg caaggaagaa
    40981 aaattgcgca gcagggcatt cgtgtgatcc tgccagatgc gatgaatcat ggggagcgca
    41041 aacagccggt ttcttctatt ccatcctata cattttggga tagtatctac ggaaatctgt
    41101 ttgaatttga cacacttatc aagcatttag aaaaaagagg tcttctacat gatcagcttg
    41161 cagttggcgg ggtttcgatg gggggaatga caacatgcgc cttgttggct aaacatcctc
    41221 atatagtagc gggcgtgtgt ttgatgggtt cccctgcacc tttggaatat ggaaaagaaa
    41281 tcgtaaggcg cgcatcggaa tataattatg tcgtaccaaa agactatttt gatttgattt
    41341 catgggttaa gagctatgat ttgtcattgc agccggaaaa gttagctgga cgtcctctgt
    41401 tcttttggca tggacaagct gacgaaaaaa tcccttatat acaaacggaa cgatttgtag
    41461 aaaaaaatcg gcataagtct tacggaaaag aaattgtttt tcgttcctca aaaacacagc
    41521 gacatatggt acaaatacca ctaatggaag aagcttccac ttttttagca aaaaatttga
    41581 taaaaaagta taaataattg aaacggaaaa tcactcatga cgatagagga ttttccgttt
    41641 tttattttgg gctataataa actctgttag gaaaatttgg tataattgat agtaagaatg
    41701 acaacatgtt agaaaggaag agagcatgcc cactttagca gatgttgcaa aacgtgccaa
    41761 tgtttcaaaa atgacggtct ctcgagtcat caatcatccg gaacaggtga ctgacgaatt
    41821 gaaagagtta gttttctcag caatggctga actggattac cgtccaaata ttgctgcaaa
    41881 agcacttgtc acgaatcggt cgcagatcat caaattattt atactggaag aaatcgacac
    41941 aactgagccc tattacatga atttgctgat gggaatcgct aaacggatcg gtaaagcgca
    42001 ttattctctc caattagtta caaatgatgc cttcgacgtc ggttcttgtg atggatacat
    42061 catcacaggt gtcagaaaaa aagacttcga ttggatcgac cgtttggaaa aaccagttgt
    42121 cttgtttggg gaaaattctg aaggatatga ttatgtagac agcgataatg aatatggtac
    42181 agcaaaagca acgcaatatg ccttagaaag agattatcaa acgattattt atatcggtat
    42241 cgaagtggac gagccttttg aaatttcaag ggaaactggt tatttatctg tgatgaagga
    42301 agttgatctt cctagtaaaa tcttgcggtt tccaaatcat tcgacagaag tagaaagata
    42361 tatgacagaa aactgggcag attttacaga taaaacttgt tttgtatgta gttcagatcg
    42421 gctagcacta ggagcagtaa gagggattat aaatgctgga ggacacttgc ccgaagaatt
    42481 tggtgtgatt ggatttgatg gggtattttt agaccaaatc agttcaccta aattgacaac
    42541 gatcaagcaa gatattatac gtatgggtga agtatgtgga gaaatgttgt taaaaaaaat
    42601 agaagaaaaa ggggcgagtc aaggttatcg tcgttttttt cctgaactag ttatcagaga
    42661 gacaacgaat taagtcttat tactatatat atatttatga aattcatcgc tattttctta
    42721 aaataatcag cgcattgcta ctgaaaacaa tgaatctgtg ctattattga gaaaagttcc
    42781 atttttgaga aggatggttt gaatgaaaga aatcagacga aaacatttcc gttttcctgc
    42841 gtacgatgat gagttaggcg taaaattaac caacgacaag aagcgagtat tatttgacgg
    42901 acaagaagat cttttgactg aacagaatca gtccccgttt gaacagatga aagaatttca
    42961 tttcacgaac acgaacaccg agaaaatccg tcgcaagcgt cgtgcaacaa atggattgaa
    43021 gtcagctccg cgtaaggaaa atttggaaaa gcataaagga aaattaccgg attataccgg
    43081 ccattaccaa tcagaaatca catcgacagg acgaaaaaaa ctttttggtg attcctctcg
    43141 ttctgtcaga caaagtgaaa caaggacaga gtccaagcat actacacgaa acgcatctgt
    43201 tgacacgaag gtgcgggagc gttcttattt tgttcctaaa tatgtacccg catcgattat
    43261 cccagaccct agagaaagca aaatttctga ggatcattta atcgaatcga tgaaaaaaac
    43321 ggatgactct tatctgttgt tcgatacaga accgacgccg tatcaagaaa aaagaaatgg
    43381 tgagccgtct gtacgaacat tcaatcgaac aaaaacgtta gaggaaacaa agcagtcaga
    43441 agaaaaaaaa tcgaaaaaaa gccatggtat cttagaacgc tcattacaag gattgatcga
    43501 tgatcaagga aactctttgg gagaaaatag ctattttaaa taagtgatat acaaatttat
    43561 attcggctac agttggaaag gaaacgctgc ggcgtttcct tattttatac tctatccgaa
    43621 agtttatatc gtatcctcaa atttttttga gtttaagcga attttttctc ccaataagta
    43681 agaaaataat ggcaaaataa aaaaacaaac tattttcgca gatcgtttgc aaaaatagtt
    43741 tattttttta taaagaagat tttactgaat aatatcattt tagtttacat aatgatattt
    43801 ctgtaaatta aataaagtag tgaaaaataa gaatcagtgc tctaactgtt caagaggatc
    43861 aagtggttca aatgtttctg tagtctgttt tgcttccaat tggggcaaaa cagatccaat
    43921 ttccagatac ttcactttat tcaatgtata agatagaata gaacagctaa gtaatgtata
    43981 cagaaaatgt gtgagatcca aataaagcaa ggagacaact aaaataccaa aagtaatcaa
    44041 gaaaagactt ttgccaatct taatatgaaa atatttttca atggcttgat aaatgatatc
    44101 tgttccgcct gtgcttccgc cgaatcgaaa gacgagaccg caaccaatgc cagaaagaat
    44161 acctgtcatc aaactattga tcagtagact atctacagca aaatgtatat gaaggctctc
    44221 ccataaatgt aaaaagaaaa ctagcgcaaa tgttccgacg atcgtccgga gaaaagcgtg
    44281 ttttccaaaa agaaacaatg agataagcaa taaaggtaaa ttaaaaagca gtgaagtgac
    44341 agaaggagcg aatccaaaaa gattatgcaa aagcaaagca ataccagtaa ccccaccatc
    44401 tgccagctta gatggaatat tgattcctgt cacagctaat ccgtagattc ctgcgcctaa
    44461 aatgattccg cacaattctg taaataaatt taattttttc atttctagcc tcttttcttt
    44521 tgaaaatttt cagcaacttc tttattatac tgacttatac tcttacttca agaggaaaat
    44581 gaaaaaaaat tatttttggc tgtttctttt tatttatcaa ataaggagtg gaaaaatgcg
    44641 agcagcagac ttattgacaa ttcttacaaa atcacccaaa aaaacagagt atcaaggagt
    44701 ttttttggct caagaacaaa aactggtgcc tgtgacaatg gtccagataa ataaagcaaa
    44761 tcaatttatc tttttatttg aacacggcaa agcaccactt gctatgaaag acatctttct
    44821 ttttttgatg aagaaccgtg agaaagctgt ttattatcag tcaaaagaag aactcattcc
    44881 tctttatgga gtgaaagaga tagaaaataa agtggtgata taagtaaaaa taagaaaaca
    44941 agacgtttat gatgtcaaaa caaccatttt ttacattttt ggcaaaaaag tgtcatgata
    45001 cagtataata ttcgttgaac aggccaataa aggtgttctc ccccacgaga tttctacttt
    45061 attggctgtt tctttttatt aaaataaaag ttgtgtgtgt aaaaaagata tagctgattc
    45121 caagtgacaa tttcgtatag atttttatgg tattcgtagt gaaaaaacga gaaccaaaaa
    45181 gttgatccga ctttgttgct tggattcttt ttttgtcagc atatttagag gtattttttt
    45241 cttttttttg ttcgtgctgt aaggttaagg caagacaagt tagttgggaa aaaggaaaga
    45301 aatgtttata cttaaagaat aacagaaagg aaggtcgcat gaataatttt atacaaacat
    45361 taagtgaaag aaaaggcgag ttgctggaag ctgcatttca acatctgtcc atttctctca
    45421 tttctttgct gattgcagca ctgattgcta ttcctttagc gatttgggca gccaatcata
    45481 aaaaaatggc agaagtctta ttgcagatca cgggtgtgct ccaaacgatt ccttcacttg
    45541 ccttattagg tttacttatc ccatttgtag ggatcggaac tgttccagca ttgattgcac
    45601 ttgttattta cgctttgctt ccgattttcc aaaacacgta tattggtcta gctgaaattg
    45661 acccgtctat tgaagaagca gcggtagcat ttggtatgtc cagaatgaga cgcttgctga
    45721 aagtagagct tcccatcgca ttacctgtca ttatctcagg tatccgaact gccttagttt
    45781 taatcatcgg tacggctact ttggcagcat tgatcggagc aggtggatta gggacattta
    45841 ttttgttagg tattgaccga aatacacctg ttttgacatt gattggtgcg atcagttctg
    45901 ccttgcttgc aattatattc agcggactga ttcgctggtt gcagcataag aaacctcgat
    45961 attccatcat tactttagca gcagtggtgt gtttatttgg cggcgtttct ttatatcagt
    46021 ctgccttgtt taaaaatgaa accatcacga ttgctggaaa attaggagcg gaacctgaaa
    46081 tcctgatcca aatgtataaa gaattgattg aagacaacac aaaaacagaa gttgaattaa
    46141 aaccgaattt tggcaagacg agttttttgt tcaatgcact ggatcaaggt caaatcgata
    46201 tctatcctga gttcacaggt actgtcctag aaagcttggt gaaggtacct gaagcaatga
    46261 aaagtgctga tttgacaaaa gaaaaaacat atgacgaagc agaagaatta ttgaataagc
    46321 aattcgatat gacattatta aaaccaatgg cttaccaaaa tacgtatgcc ttggcagtca
    46381 agagatcttt tgcgaacgaa catcaactta aaaagatttc cgatttggca gctatctctg
    46441 atcagatcaa agcgggattc acactggaat ttattgatcg ttcagatgga tataaaggaa
    46501 tccaagaagc atatggcttg aatttttcaa gtgtacaaag catggagcca agtttgcgtt
    46561 accaagcaat caataatgga gatgtcaata ttgtggatgc ctattccaca gatagccagc
    46621 tcaaggagta cgatctagtg actctagaag atgatcgagg gttattccca gcttatcaag
    46681 gcgcaccgct gatgaaaaaa gattttgcta aagaacatcc tgaaatcgtt aaagcactga
    46741 ataaattaag cggaaaaata acagaggatc aaatgattga tatgaattac caagtcaatg
    46801 tcgaaaagaa aaagccgaca gaagtagcac gaatcttcct gaaaaatgaa ggattagtaa
    46861 aggaggaaga tcagtgacta aattaatcga gttcaaacat gtgcaaaaaa agtatgatgg
    46921 aaagtatgtc atcgatgacc taaatctggc aatcaaaaaa ggagaaatct ttgttttggt
    46981 tggtccttcc ggtagtggaa aaacaactac tttgaaaatg atcaatggat tatctaaacc
    47041 atcagcagga gacatctact tcaaaggaaa atcgttaaat gaatataatc tgcagaaaat
    47101 gcggtggaat atgggatatg tgctgcagca aatcgctttg ttcccgacca tgacagtcaa
    47161 gcaaaatatt gaagtcatcc ctgaaatgtt aggttgggaa aagcagaaaa gagcagaccg
    47221 tgtagatgaa cttcttcaaa aagttggctt atctcctgat atttatcgag atcgtatgcc
    47281 tagagagttg tctggaggag aacagcaacg gatcgggatc atccgagcta ttgctgcaag
    47341 cccagatgtg attttgatgg acgagccatt tagcgcatta gatccgattt caagaaactc
    47401 attacaagaa ttggttttgt ccttacacga agaattgggc acgaccatcg ttttcgttac
    47461 gcacaatatg gaagaagcaa tcaagctagg tgatcgtatt gcatttatga aggatggaga
    47521 aattatccaa tgtgacacac cagagcagtt attgatgaat cctaaaaacg actacgttcg
    47581 gcatttcttt gatgaaccga aacagacgaa agagtggcga gtagaagatt tagtcgtcaa
    47641 tggatatttc ttaaatgaaa taccagaaaa tgctcgtcaa caagtttgct ttgatacgcc
    47701 tatgaaagaa gtatattcgc ttctttcggt gtatccaagc attgtgatag tagaaaagca
    47761 aagaagtatt gggtcgctga caagtaaaga aatctttgct tttttaagtc gagaggagga
    47821 aggaaatgaa aacatatgat gcaatcatca tcggttcagg tgtgagcggc ttaagtgctg
    47881 cttatggatt gaaagaagca ggtaaaacag tactagtggt cgaagaagat ctatggggcg
    47941 ggacttgtcc taaccgtgga tgtgatccta aaaaagtctt gttaagtgca gtagaggcac
    48001 gtaatcgggt aaaacaattg agtggaaaag gttttaacga gatcccaact gccaactggg
    48061 aagaacttca aaaattcaaa cgaacattta cggatccagt cccagaaagc cgaaaaaaac
    48121 aattagcaga agctgaaatc gatcatcttt caggaaccgc tcgttttttg gatgattcat
    48181 ctattgaagt caacgaagaa gtcttccatg cggattactt agttttggca accgggcaaa
    48241 gacctacgat cttaccagtg gagggaaagg aatacttgaa aactagtgca gactttttat
    48301 cattgcctgt actgcctaaa gaaatcattt ttatcggtgg tggttatatt gcttttgagc
    48361 tagctacgat tgccaatgct gcagggagca aagtaaccat tgtccatcat aatcaacggc
    48421 cattaaaaga atttgaggcg tctttggtag aggaagccgt ccatcaaatg gaagcatctg
    48481 gcattcagtt tgcttttggt gttgaaaccc aaaagatcat ttcagaaggt actcgctatc
    48541 gtttagtcgg aaaagagacg gaattagtag cagatatgat tttttgtgcg actgggagac
    48601 agccgaatac agaatcttta gcacttgagc aagcgaatat cgtctttgat aaacacggaa
    48661 ttgcagtcaa tgattatctg caaacgagca atccaaaaat ttttgcgtgt ggagatattg
    48721 tttcaaggaa aaccccaaaa ttgacaccag tcgccacttt tgaaggaaat tatgtagcta
    48781 agcggataac ggatgcaaca agcgaaccta tcaaatatcc gattatacct acgatcgtct
    48841 acgccagtcc taaacttgct gaagtaggag ttacaaaaag tcacgcctct tcgtcagatc
    48901 aagtggtaga gatggatctt accagttggt tcacctatca tcgtgtcaat gaaccagttg
    48961 ctaaggctga attgaccttt gaccagcaaa attatttgat tggtgctgca gtgattagcg
    49021 agcaagcaga cgaattgatc gatgatttga cactcgtcat caatcaaaaa ttgactaaaa
    49081 aagagttgga tagctatatc atggggtatc cgacattggc aagtgatctc tcctatttat
    49141 tgaaataatc agcatgataa ctaaagcgct tgttgctttt tccttcttgt gctacacttt
    49201 aaaaaatgaa tacctgtttc tggggatgtc gtagacgaca tgagagtaag ttgtgaactt
    49261 agacccattg aacctgtcag ttaatactgg tgaagggaga aacatagcag acgcttgaag
    49321 cgggttacta tgtcgtgccc atttcaagcg ttttttgtta ttcatattag aagaaaggag
    49381 gtaacaaaat gaagaaaaat ctttatccag cagtcagtat ggcattcatt ttaattattt
    49441 ggcagattgt tgtcatagtt tggaaaatac ctgcctttat tttacccgga cctgctgctg
    49501 tattttctgc attagcggca gatgcacaag tattactttc ccattctgtc acgacattaa
    49561 atgaagcagt gcttggtttg gtaatagcta gcgttctttc ttttattact gccttggcaa
    49621 tggattattg gaagttttta gaaatgtctt tttatccgtt actagtgatt tcacaaatgt
    49681 tgccaatcat ggtgttaggg ccattactta ccctatggtt tggtttcggt atgcttccaa
    49741 aagtgatcct tgttgtcttg ataagctatt ttccgatcgt agtttctttt tcggatacat
    49801 tgaggaaagt atcaaaagag caaatcatct ttttgaaaac gatgggggca gatacaggga
    49861 agatttatcg aatatataaa ataccagcgg gtttatccgg cttcttttcc ggtttaaaag
    49921 tggctgctac ttattgtgtc agcggagcag tcgttggaga atggctaagc gctcaagcag
    49981 gcttagggta ttacatgata cgcgtaaaaa acagctacca gatcgataaa gtttttgctg
    50041 cgatcatttg tgtcattcta ttgagtttat tgctgaatgg cgcttgttca ttattgaaaa
    50101 aagggtatta tcgtatgctt tatatttttt aagagaaggg gatcaaatga aaaaattatg
    50161 gcttttatta ccgctactgt tgcttttatc cgcttgtggc actgcaaaag aaacaagtag
    50221 caaacaagaa ataaaggatt tgaaaaaagt gacacttgtt ttggattatg taccgaatac
    50281 taatcatact ggcatctacc tagcaaaaga aaaaggctat tataaagagg ctggtttaaa
    50341 tgtccaaatc atcgaaccag gagataattc gacaagtatt ggtctagtag gtgcggacaa
    50401 agcacaattt ggtgtaagtt atcaagaaga tgtcacttat gctcacgcag atggacagaa
    50461 tataccagtc aaagcaattg ctacagtgat caaacataat acttctggat tcgccaccct
    50521 atcagacagt aacatccatt caccaaaaga ttttgaagga aaaacatatg caggttggca
    50581 aagtccaagt gaagaagctg tcttaaaagc tgtgatggaa aaagacggcg gggacttttc
    50641 aaaactgaca atggtaggaa gcaatggaga agggccagaa agcttaggaa aatcctcaga
    50701 tatccaatgg tactttgaag gttgggatat gatcaaagca aaagaagcgg gtattgaact
    50761 aaactatatc ccattgaaag aattagatga acggttagat tactatactc cggtaattat
    50821 tacgaatgat caactgatca aaagtgatcc agaattggta cagtctttta tggatgccac
    50881 gaaaaaaggc tatcaggaag ctattaagga tccaaatgac agtgctaaac tgttgcaaaa
    50941 atatgcaaaa gaaaatgatc ggacgttttt agaagaatca caagcattct tgtcaaaaaa
    51001 ttatacggat gatcctgaga attggggatt gatggaagaa aaagtttggc gcaattatac
    51061 agcttttatg caggaaaacg gattgatcaa gcaagatgta ccttcgcaag aactattcac
    51121 taatcagttt ataaaatagg agtgatagat atgtcaatcc aaatcagcca cttgaatgta
    51181 gtcattgaaa acctgaaaat attagaagac atccatttca ctatccctaa agataccttt
    51241 acatcgattg tcgcgccaag cggagccggt aagtcaacat tgctgaaaac tttaacagga
    51301 gtccagccta tgacttctgg aacgatcaaa gtagacgatc aaaaagtaac tggtctccat
    51361 acagcattta gttatatgcc gcaagaagat atgttacttc cttggctgag cgtctatcaa
    51421 aacgttacgt tatatcaaaa aatcaatcat ctaacaatca atgaagaaca agtaagggat
    51481 tatctggata tctttgggtt aaacggctat gaacattttt tgccggaaca attatctggc
    51541 gggatgaagc agcgaacggc cttgttacga acaatcatga atccttcttc ttacttgctt
    51601 cttgatgaac catttggagc gttagatgct atgaccagag ggcagatgca agactggatg
    51661 ttgaagctgc ctaaaaaagc aaagcggact actttacttg tgacgcatga tatcgaagaa
    51721 gcaatttatt tatcagacag gattcttgtg ctttccgccc gtccggcaca tgtgattgct
    51781 gagattcaag tgcctgaaaa acgacggtca cgtgaatggc tactgcagca atcagagttg
    51841 aagcaaacga tttatcagtt attggcaggt gaattagatg ttaagtgatg ctcattgtca
    51901 tttagatggg agtcagagtt tagcactcct tcagcaggaa cacacgattt tgacgatcat
    51961 caattgtgat tcgccagaag aatggaagga aaacagacag cttgcagcaa gtaagactca
    52021 agccttaagt tacggtatcc atccttggaa agcagatagc tatacgtttg aacaagttga
    52081 gccttttttg aaaaaagcaa ggatcatcgg tgaaataggg ttagataata tttggacgaa
    52141 tgtaccgatg actacacaaa aaaaagtatt tgagcgacag cttgcttttg cagccataaa
    52201 tgagaaacct gttgtactgc acacaaaagg atgcgaaaaa gagatcttaa attatatcca
    52261 agcttaccct aatcactatt tgatccattg gtattcttct ttggactatc aacaagacta
    52321 tattgattta ggctgttatt tcagcattgg tcttgatttg aaaaagaatc cagcagtctg
    52381 gcaactagct aaagcagtcc caatcgacca cttacttatt gaaacggatg gactacatgc
    52441 agcagagtgg gctttagggg ggattttttc agaaaaagat tatgtctcac tgcttgaaga
    52501 acagttaaag acaatcgctg agatcaaaga gctatcagtt gaagaagtta aaaaaaatac
    52561 ttatcaaaac ttggaacgtt tcattagatt aggtaaataa aaaagagatg tgacacaact
    52621 agtgtcgcac ctctttttgg ctttctttgt ataaaagtaa actgtttgat taataaatga
    52681 atcttagtac caaccgtttg cttcccagaa tgctttcgcc gcttcccaag aaccataacg
    52741 tgaagtaaca tattgttctg ctactctttc ttggtttgca gcagagtagt caccatttaa
    52801 gtaagaagaa tctaattggt aacgtccgat gtaacgtccg ttcgttgctg tgtaagaacc
    52861 gcttgattct ttttgtgcga tccattcttt tgcagagctt gtgcttgctg gagtagcagc
    52921 agtttctgtt acagcaggag caggttgtgt ttcagcaaca ggcgctgctt gtttttccgt
    52981 ttgaaccgtt tcttgtacaa caggttgttc agcttggact ggtgcttctt gaacggctgc
    53041 ttgaggttca gcagctgctt gtgcagagcc ttctgttggg attgttaatt gttgtcctga
    53101 gta
//
LOCUS       DACDYJ010000012        52831 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDYJ010000012 DACDYJ010000000
VERSION     DACDYJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.012"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(313..939)
                     /locus_tag="IWM51_000694"
     CDS             complement(313..939)
                     /locus_tag="IWM51_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970133.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1234..1569)
                     /locus_tag="IWM51_000695"
     CDS             complement(1234..1569)
                     /locus_tag="IWM51_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9970134.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1556..1840)
                     /locus_tag="IWM51_000696"
     CDS             complement(1556..1840)
                     /locus_tag="IWM51_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP9970135.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1896..3419)
                     /locus_tag="IWM51_000697"
     CDS             complement(1896..3419)
                     /locus_tag="IWM51_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9970136.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3412..4587)
                     /locus_tag="IWM51_000698"
     CDS             complement(3412..4587)
                     /locus_tag="IWM51_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9970137.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4591..4776)
                     /locus_tag="IWM51_000699"
     CDS             complement(4591..4776)
                     /locus_tag="IWM51_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970138.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4742..6436)
                     /locus_tag="IWM51_000700"
     CDS             complement(4742..6436)
                     /locus_tag="IWM51_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9970139.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6433..6906)
                     /locus_tag="IWM51_000701"
     CDS             complement(6433..6906)
                     /locus_tag="IWM51_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP9970140.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(6975..7130)
                     /locus_tag="IWM51_000702"
     CDS             complement(6975..7130)
                     /locus_tag="IWM51_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP9970141.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7264..7644)
                     /locus_tag="IWM51_000703"
     CDS             complement(7264..7644)
                     /locus_tag="IWM51_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9970142.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7648..7863)
                     /locus_tag="IWM51_000704"
     CDS             complement(7648..7863)
                     /locus_tag="IWM51_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970143.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7866..8273)
                     /locus_tag="IWM51_000705"
     CDS             complement(7866..8273)
                     /locus_tag="IWM51_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP9970144.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8539..10014)
                     /locus_tag="IWM51_000706"
     CDS             complement(8539..10014)
                     /locus_tag="IWM51_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP9970145.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(10004..10852)
                     /locus_tag="IWM51_000707"
     CDS             complement(10004..10852)
                     /locus_tag="IWM51_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP9970146.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10889..11251)
                     /locus_tag="IWM51_000708"
     CDS             complement(10889..11251)
                     /locus_tag="IWM51_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970147.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11551..12175)
                     /locus_tag="IWM51_000709"
                     /pseudo
     CDS             complement(11551..12175)
                     /locus_tag="IWM51_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12172..12483)
                     /locus_tag="IWM51_000710"
     CDS             complement(12172..12483)
                     /locus_tag="IWM51_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970148.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12527..12820)
                     /locus_tag="IWM51_000711"
     CDS             complement(12527..12820)
                     /locus_tag="IWM51_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970149.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            13014..13661
                     /locus_tag="IWM51_000712"
     CDS             13014..13661
                     /locus_tag="IWM51_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9970150.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13722..14867
                     /locus_tag="IWM51_000713"
     CDS             13722..14867
                     /locus_tag="IWM51_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9970151.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(15515..16735)
                     /locus_tag="IWM51_000714"
     CDS             complement(15515..16735)
                     /locus_tag="IWM51_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HAP9970152.1"
                     /translation="MSEKIYKTPSQRFNTLRYRDMEISTSSHRRVLNQYNYYNLINGY
                     KDPFLFTNNPEKYKIKKLPDGTSIKTTPNQLEALYKFDDALRKKLLEYLLKIEEQLKN
                     AFTQAFYEVQSKNMNIPYANRKNLHLDNEYLRRCYFDLDVHYEQSYQNRNAIRNTIVH
                     DTVPRNFYFGGDVEPLFYNSNGRKTPQGAKFRKVDKEQYYDSFKSIVYSTLAKQKSKS
                     ESLERYSSKHGYVPMWILMNFLTFGNISKFYLFQTKEVKRNILKRLKFIEETTSEVDF
                     EIISLNFSRSLEILTIYRNICAHNERVYNNSVSIPLDDSFMDFGEKLPFYYEAKSPQQ
                     MSQSKLVIRRNSRKKIFVLFFLISNFLNKREISKFKNEIETQFSLLEAKLSVITIDEI
                     KILMGLNFDWKSNL"
     gene            complement(16951..18450)
                     /locus_tag="IWM51_000715"
     CDS             complement(16951..18450)
                     /locus_tag="IWM51_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAP9970153.1"
                     /translation="MDNQEAKQFLLKPASFFNTSLPNYINLSVGIGKAVDLLKDKPFS
                     ELVKHKRSLSEAVDVNHRILANKDGNYAWRPLQILHPVVYVDLVNVITQQNNWRHIKN
                     KFEEFQADKRIECISLPVESISSKSDTAETILNWWENLEQAQINYALEYDYCIHTDIT
                     DCYGSIYTHTIPWAIHSKEWAKTHRKPSQGVGNLIDSKIQYLQHGQTNGIPQGSVLMD
                     FIAEIVLGYADKLLLNRIEESGIEEFKILRYRDDYRIFSLRKDHAEMIIRLLSEVLSD
                     LNLKLNPNKTFLSDNIIIEAIKPDKIYWDLTHSSFIEKNNSTWEFKLSLQKHLLQIKL
                     LGDKYPNCGSLSKALSEVYRHRISTLSKRPDDINQLISMIVNIMQNNPRTLEACIVIL
                     GKLFEFIAVEKINLYLDKILRKFVNTPNTDMVEIWLQRLSLIHSREKLYSAKLCKKIS
                     DPKRFSLWNSDWLIDEFDESEIIDEAYISNLSLTTPAKELELFISQYGK"
     gene            complement(18714..19028)
                     /locus_tag="IWM51_000716"
     CDS             complement(18714..19028)
                     /locus_tag="IWM51_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970154.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLNFFIQYDRIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSETFEIDPDYYQPQEVFREFRNQIIRSSNI
                     EN"
     gene            complement(19105..19599)
                     /locus_tag="IWM51_000717"
     CDS             complement(19105..19599)
                     /locus_tag="IWM51_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970155.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLKNDTEKPIRPQQW
                     SIENKDGEELYEYAMILDNPEYIESDKAETINYYYELPNEMQDYQIVVRRANGYLVGL
                     PFTL"
     gene            complement(19619..19906)
                     /locus_tag="IWM51_000718"
     CDS             complement(19619..19906)
                     /locus_tag="IWM51_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970156.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDSESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNK"
     gene            complement(19928..21265)
                     /locus_tag="IWM51_000719"
     CDS             complement(19928..21265)
                     /locus_tag="IWM51_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9970157.1"
                     /translation="MGSIIKIASKSRSIHNTISYMLNKQEDSELVTGIGVSNDLNAIE
                     DFKRTNEIFGDKGKIKLWHFTQSFDPLEDISAEKAHELGVKWAEKMFPNQSILVVTHT
                     DKGHVHNHFGISPNDQRTGKKMDIRKNDIRVLQAWNDNILIDNGISPLDMYKYNHLVA
                     DRENWKEREFRTENKISNREKIKLAVSEVAQYAKNYDDFHAELKKKHSVETYRFAKNQ
                     RIGYKLLDSEGGLILKIGGKRLGQNYDMNYLDDLFTENKKAEQIQSEEVLTEIAIRHL
                     GKSLNGRADSRINWQERAQRFELDNENIPFLRSSKDYEEFYDKYKFEIEDRIEIVLDV
                     FGENKDQFFGFAVEEEPLKYKKKCVDLVYKKVVYDISESLSTDYDQFNVHDTKPALER
                     NFKKRYIQETKRVLYLFNRAIDDGTIEKARREYEETAREIDYQKRQNQYDMDF"
     gene            complement(21250..21747)
                     /locus_tag="IWM51_000720"
     CDS             complement(21250..21747)
                     /locus_tag="IWM51_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970158.1"
                     /translation="MAIDVSKLPLEERRKRYKRNEDSRRQNRPIKFRVDDYQYQELEK
                     TADIFGLSVPMYVKKIAVDNAGKSLKYTDRQYKEINFHLLAIGRNLNQLVKTANYLRV
                     QLNKKDTEIQKEEHQSVYLSFTTKQERLKRLYAKKNDLMIQIKSISDPLNRLEKELNK
                     LWARL"
     gene            complement(21896..22234)
                     /locus_tag="IWM51_000721"
     CDS             complement(21896..22234)
                     /locus_tag="IWM51_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970159.1"
                     /translation="MARKKQKSIADLKKELAELERKLYPELGKFAYEKTGISSVKEFE
                     ETFEINQKGTREKWQKAVNFANSEFTLVHEFLASDQLLETKFQQFLRRKGETAYRIFY
                     REETQEESVR"
     gene            complement(22238..22423)
                     /locus_tag="IWM51_000722"
     CDS             complement(22238..22423)
                     /locus_tag="IWM51_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970160.1"
                     /translation="MQLILQISTHDFLELDELCESVKKATNEKITVTNYIELMIQEEL
                     KMKNELMNELSHIQKGA"
     gene            complement(22437..25112)
                     /locus_tag="IWM51_000723"
     CDS             complement(22437..25112)
                     /locus_tag="IWM51_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970161.1"
                     /translation="MKIVKEKIASRKIHWLALSILVLMFIVVFTGNVVQASIFNWFDD
                     TESQTEFLNNPLYQPYLKKFNGDFYGQFGMWIGWGVIKAMFAITDGIQNMIPEVLDLF
                     SFIENTGMNKVYQSVLSTVVIGLMILSLMIIGYKMVIGKGNLDLKSVGMNVVMSIALI
                     LLMPTLIHSGIEFSKTFYNDTTTITNSDEGVAWSLIEQGVTDLAYINKTDQYSMIDKT
                     NDRNNLTKKNFWKTDLTQVLTDNVLEKMEKENATADNLRYELVENSDNEFVASKFEDN
                     FLSTFTDGLKSGYYRYQANLWGISIGLIALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDLETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANGTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQGAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKLVKENVQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENLQSSSTSQPKTVDVRENVQNSTSSQGFKSVDVRENVQSSSASQPKTVDVRENVQS
                     STSSQGFKTVDVRENAQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIREN
                     DKGSKPRRRFTYEDNELFRDTLNDPNPLFDSLLKK"
     gene            complement(25099..25317)
                     /locus_tag="IWM51_000724"
     CDS             complement(25099..25317)
                     /locus_tag="IWM51_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970162.1"
                     /translation="MIENRLIDQICCLDQNVDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQEMAQKLSESLKKWAEGGNNENS"
     gene            complement(25310..26275)
                     /locus_tag="IWM51_000725"
     CDS             complement(25310..26275)
                     /locus_tag="IWM51_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP9970163.1"
                     /translation="MKTLFDYILTLTGGSVEFMAEKRMLSKTFLNNASFMKMSDSAKL
                     LYVYLNVNADDDGIVEAYPYVRMLGTPDDDVKILLAKEFLYMLNEDLLVFLPHWLQNQ
                     TLRTTRYTPSIYRFKLLNSNPDFFTRLKQISIEKILKAGQTEMLRNDLIEKWYSVGLP
                     KDDHGHAQVRLGQVSSDQSRSGKVSLGEIREDQSINLSDDMEVEIMRQKIKNQIGYNA
                     LKSNPCYKRIVEDIVNIIQSIYSEKSKTYRINREEKSANEVIEKVEQLNQFDIESAID
                     SFNAIDHEIHNLNLYWITTLYNCKNTVSSRAYNNFVTQTREVLYD"
     gene            complement(26465..26749)
                     /locus_tag="IWM51_000726"
     CDS             complement(26465..26749)
                     /locus_tag="IWM51_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9970164.1"
                     /translation="MDVSSVQVILPPDLDQSLRKYFYQVAKEAVDQVRIETSLSKEIF
                     RLGEACEYLSCSRSTLKKFINQRGLKVSIIGDCQFIRKEELARFIKEYEI"
     gene            complement(26762..27055)
                     /locus_tag="IWM51_000727"
     CDS             complement(26762..27055)
                     /locus_tag="IWM51_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9970165.1"
                     /translation="MTDNEINAILSLIKTKKDEVVRLDSPVRKYRRFCGYRQKELAEL
                     LEISELTYRNKEKGRVPFKDYEIERIVKLFHKDGIHLDIVDIFFSDLKTKKDE"
     gene            27124..27888
                     /locus_tag="IWM51_000728"
     CDS             27124..27888
                     /locus_tag="IWM51_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9970166.1"
                     /translation="MNLNKTSVGNRIRLVRKELGLSMKEFGKKITPTAADSIVSRWER
                     GISLPNNDRLTQIAKIAHKDINWLLWGTFKEFISSLLDKEGYTNFLNDFPETVDTIYN
                     DLMPISENLQFPDYENSILRLFNKIYLPIFNDYCLDIIELDLEDFINKRHSISSDKFK
                     NRFLSSFHEGVSNSGYKYGDTDLIYKYGIALLEKFDDSYKLNNPYESIDAYFSAMTVN
                     TEDTEKLIYTICKSLGIPFQKGSNTAQILSKNHNNF"
     gene            27939..29084
                     /locus_tag="IWM51_000729"
     CDS             27939..29084
                     /locus_tag="IWM51_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9970167.1"
                     /translation="MATIKQYEKKDGTKAWLFQTYLGISPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKKETNETFKEVYDLWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGQFRIKEITTKHVQKILNQWAKTTDQYKVLHSTASRIFKYAMTLGIITKNPCELVI
                     MPNRNVEKKKDTVKIYSRDQLESLFQYLESKKSTYRADYDKTLLRFLFFSGCRISEAL
                     ALDWSDIDFNSNTVTINKTLSQTKYGYKISSPKTRNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
     gene            complement(29169..29474)
                     /locus_tag="IWM51_000730"
     CDS             complement(29169..29474)
                     /locus_tag="IWM51_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP9970168.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(29499..29930)
                     /gene="ruvX"
                     /locus_tag="IWM51_000731"
     CDS             complement(29499..29930)
                     /gene="ruvX"
                     /locus_tag="IWM51_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP9970169.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(29927..30193)
                     /locus_tag="IWM51_000732"
     CDS             complement(29927..30193)
                     /locus_tag="IWM51_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP9970170.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(30448..31173)
                     /locus_tag="IWM51_000733"
     CDS             complement(30448..31173)
                     /locus_tag="IWM51_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP9970171.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(31534..31604)
                     /locus_tag="IWM51_000734"
     tRNA            complement(31534..31604)
                     /locus_tag="IWM51_000734"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31570..31572),aa:Gly,seq:ccc)
     gene            31721..32473
                     /locus_tag="IWM51_000735"
     CDS             31721..32473
                     /locus_tag="IWM51_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP9970172.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            32489..32968
                     /locus_tag="IWM51_000736"
     CDS             32489..32968
                     /locus_tag="IWM51_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP9970173.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(33219..34799)
                     /locus_tag="IWM51_000737"
     CDS             complement(33219..34799)
                     /locus_tag="IWM51_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP9970174.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(34792..35535)
                     /locus_tag="IWM51_000738"
     CDS             complement(34792..35535)
                     /locus_tag="IWM51_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9970175.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(35711..36487)
                     /locus_tag="IWM51_000739"
     CDS             complement(35711..36487)
                     /locus_tag="IWM51_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9970176.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(36735..37571)
                     /locus_tag="IWM51_000740"
     CDS             complement(36735..37571)
                     /locus_tag="IWM51_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP9970177.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(37582..38511)
                     /locus_tag="IWM51_000741"
     CDS             complement(37582..38511)
                     /locus_tag="IWM51_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP9970178.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            38703..39056
                     /locus_tag="IWM51_000742"
     CDS             38703..39056
                     /locus_tag="IWM51_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9970179.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(39130..39786)
                     /locus_tag="IWM51_000743"
     CDS             complement(39130..39786)
                     /locus_tag="IWM51_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9970180.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            40012..41946
                     /locus_tag="IWM51_000744"
     CDS             40012..41946
                     /locus_tag="IWM51_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9970181.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(42032..43255)
                     /locus_tag="IWM51_000745"
     CDS             complement(42032..43255)
                     /locus_tag="IWM51_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP9970182.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(43249..45147)
                     /locus_tag="IWM51_000746"
     CDS             complement(43249..45147)
                     /locus_tag="IWM51_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP9970183.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(45141..46259)
                     /locus_tag="IWM51_000747"
     CDS             complement(45141..46259)
                     /locus_tag="IWM51_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9970184.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(46342..47175)
                     /locus_tag="IWM51_000748"
     CDS             complement(46342..47175)
                     /locus_tag="IWM51_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9970185.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(47168..48085)
                     /locus_tag="IWM51_000749"
     CDS             complement(47168..48085)
                     /locus_tag="IWM51_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9970186.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(48312..49436)
                     /locus_tag="IWM51_000750"
     CDS             complement(48312..49436)
                     /locus_tag="IWM51_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9970187.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            49619..50527
                     /locus_tag="IWM51_000751"
     CDS             49619..50527
                     /locus_tag="IWM51_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9970188.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            50668..52281
                     /locus_tag="IWM51_000752"
     CDS             50668..52281
                     /locus_tag="IWM51_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP9970189.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        16092 a        10377 c         8269 g        18093 t
ORIGIN      
        1 atagttcttc atatttcgcc gtttttcgag cgaatgccga ggggcagctg attgaacatc
       61 gtttatttgg aaaaagggat tgtgacaaga cgtttgtcac aatccctttt tttatatctt
      121 attaattcat tcttttgttc tattcttctt cttccgccat gaatgagaaa cccatatcaa
      181 agaactataa aggttatcaa aacagcgttt atgactaaat gaattagagt tatctatcac
      241 tatttttcaa gcgatttaaa ccaaatggca tacaatatgg catacaaaaa cttcaataag
      301 ccgtgggggt ttttaaattt cttcttgttc tttaaaatca atgagtggag ctacataatt
      361 tgaatccact ttttcaaacg tcactttttg catcgacgaa tgttttcttt cccaagataa
      421 cgtaaataga tttgactctg gacttataaa gtctacttca ccttctagta ttactttctt
      481 atgcaaatat tcctctaata aatatagact tgctaaagca tacaacacat tttccaaatt
      541 tgcttcctta taatttgaag aacgaccatg ttttactctg ttatatattt tccaccaatc
      601 tggtgtatct tcaggccacc catctccaaa aggttttagt gaagtcaatg ttaaagaatt
      661 cataacccta acaccattat ccaaacacat taattcgcta gatttaataa cagctttaaa
      721 atcagttatg cttttattgc tactgctatc aaatccgcaa agttctttga aaataatctc
      781 acactccgaa ccaatcgcta gtagtagttt ttgatattca atagagaatg ttttgaaatt
      841 attctcatcg agcatcacaa aatctgtggt attaataaac tcattttcta aatgtagata
      901 atacttccaa taaaaatttg taaactctgc tttgtccatt aaatcacctc ttttgaatta
      961 taacaaaaaa aacacgtcca caacagacgt gttctttgat ttctacacaa tcagcttgcc
     1021 aaaaccatcc aatcagtcct agcatgcctg tattaaaatt gaacgttcat catctatcat
     1081 ctttcttagt ccagcatgtt tgcaaccaca cctacaattt tcagtccaac aatagatttc
     1141 gactagctaa gtcttgcaaa attcaggcag ctaacccttc atttactacc ttaattatac
     1201 taaataccca caacgtttac gagtgtaatc attctaagaa atctgtttag ctatgatcgt
     1261 tgtataatct ttaaacgccg ttccttcatt gattgaaata attttataac tcttgttttt
     1321 ccaagctact ttcatatcgt ttgttagttc ataatcttga tcgtatcgaa taataaacgt
     1381 caacgttcct tcaagaatgg tacctacact agcaagcacg tcttttagca tttgtgaacg
     1441 cacattagcc caacattcaa atacagtttc ttcactatcc accatctgcc catcttcatc
     1501 ttttacccgt tttgtctttt taaacttgac tctctcagtc aattcattta cattcttaat
     1561 gattggcata ataagcacct ctcatttgtt gtactaatgc ttgtaccgtt actggtatcc
     1621 gttcgctgct ggcttcttga cggttcaaat accaatgttg agtaagtaat gacaccgcca
     1681 aatcaaattg ctgataatta tccatatctt tatccgttgc gctactatca atagcacttt
     1741 ttatatattc gcctgctgta tcaatcaatt gttgaataaa gtcatcatca atagtgtgat
     1801 cgatccgcat actttttttt atgctatcca aaattaccat tttatctctc ctaatgaaaa
     1861 aaaggcagtg ggataaaccg ccgcccaata tttttctata cttttggtgt tacaactggc
     1921 gtaaactcaa tatacactga agcattttga tcaattttct tataatcgtt gcgaacgatc
     1981 actgcaagtc cttgtgagta gtaatcgaat ttttcccatt gagtcgttac ttgatttcta
     2041 cgagcaacaa agatagattg tgcaatatcc cccataatca tagggaatgt tccagcttta
     2101 ggattggcaa acaatgtatc tgcaatcaat actactggca taccgaataa tgatttacca
     2161 ctaggtgctg tcacatcggg ttgtaaaatg taacgtccat ctgaatcttt caatgtatct
     2221 aggtggtaat atccgctttg gtttagaatt accattttat ttaatgctgg gtctaatgcc
     2281 acattgtata gttgtttcaa atcgtccaac gtagcagccg ttttcttagt gaatgttttt
     2341 aataaatcca tgatatgctt attgtcagtg ttttctacca atttcgctaa ctgatctttg
     2401 acctcttgta caatattcac tgctgaatct tccacaacct catttgataa ggcaatctta
     2461 ccagcacgag tttctacttt ataatcaact gaagtaaaca tttcagcgtc aatatcacca
     2521 atttcagcaa gttcagcttt tgttgctaac actgcttgtt ggttagttgc tactggatat
     2581 ttaccttgtc catttgatac tgtttttact gttgcatatt gagctaagtt ataatttgaa
     2641 cgtttcaaat caaagacttc accgattact tcttcgggaa caactgccgc tgcatttaca
     2701 gtagtgactc catcacgcac ttcgccttgt gaacggatat attcttcata cccacgcaat
     2761 tctgtttttt cgttatcgat taaagttttt tccatgttat tactctcctt ttgtgcttta
     2821 gaagtggatt ctaagctatt gtttgtttgt tttgcttggt tgttactcat aaacgattcg
     2881 taagaacgct tatttacttg gacatttgat gaatcataag cgggaacggt cacgacacta
     2941 atttcattaa gtgctttgat tttatctatt gatcgttcaa ttgtgccatc ttcttttttg
     3001 tcgaatgaat ctaatccaag cacgaaccca aaggacatag aatcaacaac cccttttgag
     3061 atattctcat acacatcatt tgaataggtc gtgtcggtta gctctgcttc aaaatgtaat
     3121 cctgtatcat cgacattcaa tttcaacgta cctgctttga cactagctag tggctttgaa
     3181 taatcgtgtc catataataa gaacacattt gataaatcca cctcttttaa tgcttcgggt
     3241 gtaatcactt ctacaaagcc gcctaaatct tttgacggtt gcccgaattt caacgcatag
     3301 cctgaaatag ttttgccgtc tgttggttgt ttttcatttt ctttctctac tttgggagaa
     3361 tcggctgtta gctcagcttc ttctgtcagc cgtttttcct tttcttcttc attattcaac
     3421 tggtgtaact ccttcctgtt cttttaattg atatttctcc aacgtatcta aatacgtaaa
     3481 gttcaaactt gctagtaaac gatcgccacc atccatgggg ggtagcccca ttttcgaccg
     3541 cccctcatta atcgtgagaa gtgacccttt gacctgatcc aatacatttt ttacttttgt
     3601 ttcaggatca gtctctagta actgatcgga attaaagcgg atattggttt caagtttcgt
     3661 atctaattca ctcacaaaca cattaaagta atgaatcagc gtactttgga tatactggag
     3721 attactttga acggtgctag aatgttcatt ttcaacgcct aaacgctcaa tcggtacacc
     3781 aaacgccttg gcaatttgtt ttgtgttcca atcattggaa ttgataagct tcaaaacatc
     3841 ggtgtttatt tctaatgttt tgtagtccat tgtttcatca agaatgatcg ttctaagagc
     3901 attatctcca ctacttgaac cgttggcttc ttcaaacttt tcacgaattg cgttctttgc
     3961 tgatccgtct aaatctgact tatgaacctt caaaatacct gacccactga caccacgagt
     4021 aaagaaattg tgcaatgtgc ggttgccagc ttgttgaatt ttcagttcat cacgtaacgc
     4081 ataaagtggt ggtaatcctg ttaaaccatc ttgggtgaaa tatttgaagt gcaaaatatc
     4141 gctagacttc acacgtcttt ttttatcacc aatctgataa gacaacgtac cattatctaa
     4201 ttgagtaacc gttacacttg agttaggtaa taagtgaatt tcttctacct tttcaccgtt
     4261 gcgtttaatc tctgcaaagc tattaccgtt tagcaacata ttgaccgcca aagcaaactt
     4321 taaatgccaa ccatccattt ctgaattagg tttctcgttt agtaacttca ccagttcatt
     4381 atcagcttgt ggcatattgt ttttgaccaa ttgaatcgga cttgaagcaa tatcactagc
     4441 aataattcga acggctgcaa acacatcact atttcttaat gcacgaacgc ttgtaaagct
     4501 ggtggtgtaa tcatccgatt ggattgatac cacatgttgt aggaaaggat ctcccgttgt
     4561 attttgtctc gctttaaaaa atgccatctt ttaacctccc ttctgttctg tatttagcat
     4621 gaccgctacc ccaataagca ctatgcccag cgctaacaag ccatagtaga catttgttag
     4681 taaggtgata gcaaccaaca cacacaccaa tccaagaagt aaaagaacgg tatgaatgta
     4741 attagaaaga aaattcttca cttgtataaa attcattgtc tgctttcgct ccttccgttg
     4801 tggtgaagta atccattgca aacacatagg cattgattaa tgcagctatt ggatcaattt
     4861 tgttactgtt ctttgattta ttaatctgaa tgccgttgtt atccgttttt aaaatagcgt
     4921 tgttcactgc atgggtgaga atcgtatttt tattgtgaat aacgttgcct tcataaactt
     4981 gttctctaaa agttcgggtc ggaacgttta gagtaatcgt tccttgtctt acttctaaca
     5041 ttgggtagtt ggctttttca gctttactga taagcgaatt agcgttatac gggtcataac
     5101 aaatccccat acattccaaa tcattttctt gaatcaaatc ttgaataaac tgaaatactt
     5161 ggtcatagtc cacgattcca ctttctaatt gagtaatgga acactcacca gctttttcta
     5221 gttctcgata aggcaaaccg tcgcgctttt ctttgtcttg aagcccatat ttggtggcta
     5281 caaagctatg tgaatcacaa taaagttcgc cgttatctag cggtacgatc catgaaacgc
     5341 tagttaaatc atctgtttta gataaatcca ctccgatata aacgggcttg cctgtaatat
     5401 cttgtggttc tacttcggtt gcctgccagt cttcagcaat catatagctg tcctcactcg
     5461 cttgtctcca taaattgaag tttttcacca atacagaatt aaggttattt tgtttcaaag
     5521 caaggttcac atcatcttga atggttggaa tcattacttt tttgatttct tcactttcaa
     5581 aaatcggatt ggctttgatc catttctctt gatcgtgaat ttcttcggga tcgtccaatt
     5641 cccatatcgc tataaagtac ctgtcggctt gttcttcgcc ttttagcaca cgatctaaca
     5701 ataagtattc ctcgtacata ggaacgttta aatcaagccc agcggtacta ataacacaca
     5761 ataagccgtt cttttgttgg gtcattcctg acttaatgac gttgtacgtt ttacgagttt
     5821 ttgcttcgtg ccactcgtca agaattgcgg ttgttggtgc aaacccatct agcgtgctgg
     5881 tttcactagc cagtgccata gcgaacgaat tagagggcaa gtgctgaatc ttagagttca
     5941 taattttcag ttgtggtctt aaaaacttac tagacttgac cacgtttcta agtgaattag
     6001 acagcatatc atagcccaat ttagcttgtt tcaaagcgtt acttacaaac aatacttgcc
     6061 ttgcttctgc tggttcttgt tccataatca acgcattggc agccatgcca cttgcgatat
     6121 acgttttccc attcttacgg ctcatactgg taaacgctcg attgaatcgc ctgtaattcc
     6181 cttctttagt acgccagcca taaagactgc ctagaatgaa tttttgaaat cctaacatct
     6241 tgattgcttt accgtccgta gatggtagca actcaatgaa tttaatcgct ttggtggctt
     6301 gttcttcatc gaacacataa gggaagctat ctgacttaga acgctccaaa tcgcttatat
     6361 gacgctcaca cgcttggata attttcttgg gtgcaatcag cttacctgat aggactttct
     6421 caatgtaatc attcatgaat tcatcaactc tttgaacggg tcttcgggtt catcattttt
     6481 aaccttgttc aaagctagtt ttgatcgtga ttctaaggtc atacctagtg aggtggcttg
     6541 ttttttcaaa tcactcatag cttgtgattg aacccgaacc gctggattgg ctttctttac
     6601 tccgttttcg catgtgataa acgtacctga ttttcttatc tcggcttcgg ctgttttcat
     6661 tcgagcaaag gctaaacaat aggctaccaa tgaaccataa tctgcttcac tcaatggata
     6721 atcttttttt aatgttggta cgatacgatt ccattcactt ctagcgctcg ctgccatcca
     6781 gtcagggggc gtgatattta ttaatggttg ctgcttgaac aattcttctc tagcgtccac
     6841 acggattgct ttttcttcac ttgatatatt gccttttgta tcgtgtaata ttttagggtg
     6901 tcccatgttg tcctcctttc agacaaaaga aaaaggaacg taagcaatta agctcacatt
     6961 ccttcgtttt tcgatcagaa tttttcttta tctaatttat ctatataaat gatttttccg
     7021 ttctgcattt gaatggtcac ttgaccaaaa tgaggtaact caatggtgcg ctgtttccct
     7081 gttttatcag taataacaat tgtatttttc aataaatctc ctactttcat tatatacttt
     7141 ttcacttgtg attacaagtg taatcgttat ttgtaattac ccttatgcct tttcacaacg
     7201 gttatttgta ccaaaatgag taggcatcga ttgtcagtaa gtttcacact atgccccctt
     7261 aactcaaagg gtactctttt ctcgtctttg tctttcattg gctgtcttgg aattatggtg
     7321 tgcatggcat aaagaacgaa aattagtttc atctaaccgt ctattccaat catcttttag
     7381 ttcaattttg tgatctacaa catcagcttt ccttatcaag ccttcttcta ggcaatcctc
     7441 acatactgga tgatttaacc gatacaagta tgaagccttt cgccatgttc tactttgata
     7501 gaattgaaaa tatttacctt cgctttgctt tctttcttca cgcttaattg ccgtttgctt
     7561 tgctttatgc ttcccacaat acttctgcct ataatcaatc aatgttttac atccagcatg
     7621 attacattgt tttttaactg ccatagatta ccaaccaaag cgatctcgtt cgtcagaatc
     7681 atcttgttcc cattttgatt tgtattcaat ataaggcgaa actatcttca agcacgttcc
     7741 gtctttaaaa ttaactttag tgattttatc ttcctcaaaa ctaataaagt ctacatcttc
     7801 ttgagtgttt gaatactcaa tgtgtttctc accatcgaag tatctaatac tttccatatg
     7861 cataattatt ctacctccaa taaattagtt atcaattcat catctgttag tagcaaagcc
     7921 aagaacttat catggatgtt taaaaacaaa ttatcctcat tcgatgtaag ctctcgttgt
     7981 ctcgctcgat catataggtt aagcagacaa ttaaattcat ccattagctc acctgtatca
     8041 tcatagttgc cactatctac caatgatccc actactagcg attgacacca cgctactgac
     8101 tcctgcagcc aatcaggttc ttcttccatt atctttaata cttctggatc aaatgtggaa
     8161 taatcttctg ttaccaatcc cacactaaat gccagcgtgt tcattagaaa ccgatccatt
     8221 tcatctgtat attgaaagtt cggtgctttg tttgttgaaa taatattgag catttacttt
     8281 cctccttgat tcgtaacacg tggtaacacc tacgtaacag gtacccgtta ccactcaatc
     8341 ccaacgggga caagggttct agcgttttgg taacaggtaa cgggtaaaat ccagtaacat
     8401 tattccccag catattagct aactatttca aaagttttaa ggaaataagg tgttaccccg
     8461 ttaccaaatt gctgtatccc ttggggcagt aagggtaaac tagtaacggg ctaggtgtta
     8521 ctgacccgtt actacccgtt actttttgta ctttccagct cgatttttat tttcataggc
     8581 atttgactta ttgtaaaatc ctcgtcgttt atttcttttt ggcagttgct tccgttccca
     8641 accaggaata ctgctaataa tcaaggcaat ctttttagtt tctgcatctg atcgaccatt
     8701 caataaatcc tttgcttgtt tatcaaaaac agcttccaaa atatcagcgg tgagtacgct
     8761 atcaactaaa taaaactctt gttgcatagt agctccatta tagaaatgct gtaaattttt
     8821 ctcattacat ttactgtcag gatagttctg ataataagaa cgtctcgccc acgctggagc
     8881 ttcttcccat ttatacggga tttccatttc caaatattta gtgattgctt ctttaattgg
     8941 attctccatc ttggcatcct cttgatagtc ttttgcaatt tctaagactt ctttatcttc
     9001 atcgggacta aaatagattc gttgtccttt atcaaaaagc acctttgctt cagctaacac
     9061 ttgaagaaaa tattcatcct ttgttttaaa cggattcatc tttggcttat tcttacaagg
     9121 caaaggataa aaacggcggt ttcctgtatc atctttcaaa tactgcccat cattcgacgt
     9181 tccaataaat acacattgcc ttgcccaagc tatgacattc cgaccataag gaggtctaat
     9241 tttatcgatc ttggcactga tgaagttttt tatctgttca attttagttt tgttcattga
     9301 agataattct ccaagttcaa taattacatt gccttgaagc tgcatataat catctttctt
     9361 ttcacctagc gaatctaatg aatcggtgaa aaaatcatcc gtgtaaagta atgctggaac
     9421 ggtactctta ccaatccctt gcggtccatc caaaactgga acgatctcaa actttattcc
     9481 tggtttatag atacgtgcga ctgatccgac tatccaacgt tttgttactt ctcgtgtgta
     9541 aaggttgtcg tctaccccta aaaggtcaat aaacagtgtt tccagtctag gctgttgatc
     9601 ccatttaact ttttcgatac gttcttttac tgggttgaaa acattctttc tagccacatt
     9661 tactactgct ttgtgtattt tgttgtcatc aaaaactaca ttgtagtttt cttcaatata
     9721 tgccgttaag gcatcctcaa aactgtcttc ccattctcca gcggtacgat tgatccaagg
     9781 agaatctttt aataggtgaa tgtatccgct aaattcgtta taagcaattg ttccagctaa
     9841 attgctatca ttggttaaaa tcaattctgc attcacaaca ctgttacttt taatctgatc
     9901 gtctttattg catcttaatc gtgacatcca ggttttcaat tcaatgatat tgtcttcacg
     9961 ctcgctggct tcgggaaagt ctaaaatact atctaattta tgcttatgag ccatgagctt
    10021 tccccctcaa atctttttta atcatcgatc gaataatttg attcacttct ttatctttta
    10081 gcggtggttc attgtacgca tttgctgtat aaatcaattc tgctaaagtt tgattatcta
    10141 caccgtgcca tagtaaccaa ccgcataact tagttaaatg atcgtttcta ctaccttcgc
    10201 cagcgccttg aacaatcata tctaattttt ttgctgtccc tgtttttgct acgggtcggt
    10261 attgagtctt tatttcttgc ggttttttta gcagtaggtt cactagccaa tcgggagctg
    10321 gtgcaatatt atttagagat acgttttcaa taggttcata tgctctccca acacttcttg
    10381 aaataatcat agttggcgct attggaactc ctttagtgat aatatcaatc ccgcttaaac
    10441 ttttaccttt atcatcacgc ccgaagatat cagcaaatgc acctgtttct tgttggataa
    10501 ttacaccatc aggaatttta aagaagaaat ggattccttt acctggtgtg atttccgtat
    10561 aagtagaagg aaatggatca tacattgaag caagtttttt ataattctca acgccgtttc
    10621 gtgtttcaga atgttgatct aggtcaacta ccaccatttg atgatcttca agcattaacc
    10681 caatatttct agttgcatct tccgtccacc attcattaat ttgattagaa tcgttagttg
    10741 catcattcca accactagag ccttttattg gaattttagt tccaggagat aagggataaa
    10801 ttgaccatcc cgacttttgt aattccgttg ctgcttgata attatttgcc aataaacctc
    10861 tcctcctttt tcaaaatact ggtataaatc attcagacac ctccatgagc ttcaactgct
    10921 caagttcgta tttcaaccca tcccgaacaa attcagcacc ttttaattga ttctgatact
    10981 ttacccatgt attaaatgtt ttcacccttg cttcttgcat atcagtaatg tttaaccatg
    11041 cggttaaaat ctgatttttt agctcgttga tttctcgaca aattattttg tgttctgcat
    11101 gttttttgag tacatacaaa actgttagtt tttcattgaa tgaatttcca aagtaggttt
    11161 tcccgatatt attttctagt tcagcgatcc cttgattaat ttcctctaat tcaaggctat
    11221 cgagttggtt taatagtttt gttttgtaca tctacttatg ccctccagtt cttaattgat
    11281 agtcgtactc gtcataagca gtgaagagat ataacgctaa tcctgcaaag atagctattt
    11341 ttgcaattaa tggtaataag ccgaaaattc cgccgattaa aatgcttcct ataattaagt
    11401 ttcgtttcat aatggttccc tccgattttt taatatggta taatcggaac aaagaatatc
    11461 gggttttatc ccaattaagt tgcctttatc ctgtccgcca agactgttat ggataaggct
    11521 ttttctttgt tctcgatcat gttaatgctc ctaagttgtc tgcaattcag cagaacgatt
    11581 ataaattttc tcactcactg aaaactgcaa cccatatttt tctttaacag tcattaattc
    11641 tactgtctca tctaaaatag gttcacgatc aaccaacatt tcaggagtca tatctttctt
    11701 ttttaccatt ttggaatgac cataaatagt tgatacagct ttattggcta tcacattcgc
    11761 tttaatgtag tctactggtt ttggttcagt aatagcgtgg cttaatttag tcattgcttc
    11821 tttttggtgt tctttgtcta gcatgcggaa tacttgaaag ccttcaagtc ctgtggactg
    11881 gcgtagttct ttgatgatgt cgaaaatcca gtcttgaaat gcctcggctt cttttttacg
    11941 gcttttgaat gccatgcggt aaatattttt tttcgttgat gatgtttaca tcttgtgttc
    12001 tgcccaatga atcgatgacc ttactagtag taaccccatc tttaggcaaa gagtgaatgg
    12061 ctctagtaac ctgtttaaga cctaacgctt ccgcaacatc tttcgccact gcccaccatt
    12121 catcattaat atcgacaaat cttataatgt gtccgttcca aatttcagtt ttcattcaac
    12181 gtcctccagt tcaagcccta gcaagctcgc taacaactca acttgctgat acaaaatttc
    12241 ttgatactct tcaatagaaa gtcgttcttt aaactctgct tgtactaaat cgttcaagcc
    12301 acgcaatact ccgcttaccg ttggttcttg tggtagcaat ttttgatagt ccatttgttc
    12361 gtacaaacat tcaaacgttt cttctagcgc tttgccttcc aagtttgcta attctgccgc
    12421 tgtgtcttct gtaagaattg ttgataatac tttttcgatt gctttatttt gtgtcgattc
    12481 cattattatt tcctcctatt aatccctttg cccccacggc ttgcgtttat ttttggtatt
    12541 cttccataaa gcgatcacat tcatcttgtg aaattcttac gactccatca actataatca
    12601 actttaaacc gttaggaaca tatttctgca tcatagtatt gtatgaaacg ttcatatact
    12661 tagcagcttg tgccttgttc atatattttg gaaactggta ctttttttct aataatgaaa
    12721 gtaacgttcc ttgagtttgc tctacttgtt gaacaactcg atcgtcaata attttaaaag
    12781 ctggatcatc aagcgtgagt ttgtcaattt ttaattccat atttatcccc cctcgttagc
    12841 atacatttct atacaaagat tttaaaaaaa tgcaagaggc tcttgaatac atttctatac
    12901 ctcttataaa ctcaatatac catcatacct tttcatttgc aagaatttta acatactttt
    12961 ttgaactttt atgtttcaca catatttaaa tcgtgatata gtagagaaaa tatatgaatg
    13021 gtggtgaaaa tgtggctaga aaaaaatacc ccgaagcagt ggagacagga caaagaatta
    13081 gactggctag aattgaaaag ggactctcaa tggaacaact tggaggtaaa ttatccccac
    13141 cggccagtaa aggtgccgtt agtaattggg aaaatggcta taatcttccc aataacacca
    13201 ggcttaaaca actttcaaag gtattagatg tatccactac ctatctatta aacggatctt
    13261 atacattaag agatatgcat atgatgcccg aaattaagcg aaatgtgctt gaaaagcaat
    13321 tgaaaaaaac tattgaaaaa aataactcta taattgattc taatctttct aatgctatca
    13381 ataacatcga agaagataac ctgactgtct cacagaagat ttatttaaca aacgcaataa
    13441 aatatcttaa aacaggatct cgaattgaat ttcctgacgg gagtacccca attgcttatc
    13501 tcgcagctct tctaaatacg cttttacgat atgactcatt aattaatgat tcagaaaaaa
    13561 atacagatga gaaaaaagag tatcaagaat tttatgaaaa tgatcttcta aaagagttca
    13621 cagctcttgt tactgagcta aataaacact attcaaaata ataataaact gcctcctcca
    13681 accgccccca cggcttgcta cactatggag ggaaaactat tatggcaaca ttcaagcaat
    13741 atgaaactaa aaaaggtaaa ttttggctct atgaagccta tcttggaatg aacaaaatga
    13801 ctggcaaacc tgataaggtt cgtagacgag gttacaaaac aaaaaaagaa gcccagcttg
    13861 ccttgtcacg cttgcaagtg gactacgata aaaacggtat taaaaaagcg aacaacgaaa
    13921 cgtttaaaga agtttatgac ttgtggctag aaacatataa aaccactgtc cgagaagcca
    13981 ccttcatgaa aactgaaatc aagtttagaa agtggattct tccaaaatat ggaaatcttc
    14041 gtgtgaatga agtcacggta aaaaaagccc aacaaattgt aaatagctgg gctgaaacta
    14101 ctgaccaata caaggtactt cattcaactt ctaagcgtat ttttagctac gctatcaatc
    14161 ttgggattat tgagactaat ccactagaca acgtattaat gcctaaaaga accaccacta
    14221 agcataaaga aaaggtcaaa gtttacagta aagaacaact gatcaccttg tttgattacc
    14281 taaacagcaa acctgaaact tatcgaaacg actttgataa aacgttacta cgctttctct
    14341 tttatagcgg ggttaggatc agtgaagccc ttgcattgaa ttggtcagac attgattttc
    14401 aagaaaatac ggtcaccatc aataaaacac taagccaatc caaaaatggc tataaaatca
    14461 gtgatcctaa aactgattgt agtgctgggg ttcttccact agatgaaaga acaatttcta
    14521 tattaaaaaa atggcagatt aatcagcgga aatatatgct gacacttggt gtaactgatc
    14581 cgactatgat tttttgtggt atctataaag aactaatcac acaccacgct atttatgcaa
    14641 gattgattac tatttgtgaa aaagctatgg ttccatttct cggtgtgcat gtagcaagac
    14701 acactcatgc ttcaatgctc ttggacgctg gagctactat ggtagaagtt caaactagat
    14761 tacggcatac aaaaattagt cagacgatcg attgttacgg tcacttggca aaagaaacga
    14821 aagaaaagac tgttgaaaaa ttagtacaac acttaaatat tgactagatg gcatacaata
    14881 tggcatacag taaaaaataa aaaaaggaga aaccctatca tatcaaggtt tctcctagct
    14941 ttatagtcta ttcttcttct tccgccatga agttaagttc attgacgata attgaagata
    15001 gttgataatt aaatgatttc tgaatagagc taatcacttt attgatgata attcatcttg
    15061 actggttttc taatcgtatt caaaaacgta ttcaagttgc gtggtgtgct tttgatagaa
    15121 aagactgaag aatctgtcaa aataaaatat gttaccaact gatccttcca atgaatcttt
    15181 tgggaatcaa aggaaagaaa taggctttta tataaagatt tcatttaaca aattgccata
    15241 gactcacagt gtacattatc agatactatc atcgagaacg tagccaatat cgtaaccaaa
    15301 acagcaggta gagaggttat ttggagattc aaattaattc cattgttctc atgaatttaa
    15361 tctcatgtct gattaataaa gatagtcaat accttaatgt tttaatgtac attctataat
    15421 agctaacatg gatcattttt ctgatcttat tcaaattgct agcatacttt taatagaata
    15481 ctacaaaaat attttaactt cttttttctt caaactataa attacttttc caatcaaagt
    15541 taagtcccat taatatctta atttcatcaa tggtaattac agatagttta gcttctaaaa
    15601 gagaaaattg agtttcaatc tcatttttaa atttactaat ctcacgcttg tttaaaaaat
    15661 tagagattaa aaaaaacaac acaaaaatct tctttctaga atttctacga ataaccaatt
    15721 tcgattgact catttgttgt ggcgattttg cttcataata aaatggaagc ttttccccaa
    15781 aatccataaa agaatcatct agaggaatcg aaaccgagtt attatataca cgttcattat
    15841 gggcacaaat atttctatat atcgtcaaaa tttctaaact tctagaaaaa tttaaactaa
    15901 ttatttcaaa atcaacttca ctagtggttt cttcaataaa tttcaacctt ttaagtatat
    15961 ttctttttac ttcttttgtt tgaaatagat aaaacttact gatatttcca aaagttaaaa
    16021 agttcattag tatccacatt ggaacatagc catgcttact tgagtatctt tcaagagatt
    16081 cgcttttact tttttgttta gctagcgttg aatacactat tgatttaaaa gaatcgtaat
    16141 attgttcttt atcaacctta cgaaattttg ctccttgtgg tgtctttctg ccattagaat
    16201 tataaaagag cggttctaca tctccaccaa agtaaaaatt tctgggaaca gtgtcatgta
    16261 ctatagtatt ccttattgca ttcctatttt gataagattg ctcatagtgt acatccaaat
    16321 caaaataaca tcttctcaaa tattcattat ctaaatgaag atttttgcga ttagcgtatg
    16381 gaatattcat atttttagat tgaacttcat aaaaagcttg agtaaaagca tttttaagtt
    16441 gttcttctat ttttaacaag tattctagta gtttttttct gagtgcatca tcaaacttat
    16501 atagagcttc aagttgatta ggtgttgtct tgatagaagt tccatcaggc aatttcttta
    16561 ttttgtactt ttcgggatta ttcgtaaaca aaaacggatc tttataacca tttatcaaat
    16621 tataataatt atactgattt aaaacgcgcc tatgtgaact agtactaatt tccatatctc
    16681 tgtatctcag tgtgttaaac cgttggcttg gcgtcttata gattttttca ctcatctaat
    16741 acatatctcc caaaaaaatg ggaacaaccc gcaggttatt ccctcgatca ttatttatac
    16801 cttcattata ccgagctttt caaaaaagtc aactattgct atttatacta aataaaatag
    16861 tttccaaact aagcaaatga taaagtaaga caataaagaa gtaaattttt ggatactttt
    16921 ttattagttg tagtccgtta ttcaaaataa tcattttcca tattgtgaaa taaataactc
    16981 taactcttta gctggagtag ttaagcttaa attggagata taagcttcat caattatttc
    17041 actttcatca aattcatcta ttaaccaatc agaattccac aatgaaaatc ttttaggatc
    17101 agaaattttt ttgcacagtt ttgcgctata gagtttctct cgtgaatgaa ttaaagaaag
    17161 cctttgtagc caaatctcaa ccatatcagt attgggagta ttaacaaact ttcttaagat
    17221 tttatccaaa tataagttaa tcttttctac agcaataaac tcaaaaagct ttcctaaaat
    17281 cacaatacag gcttccaatg ttctaggatt attttgcatt atattaacta tcatactaat
    17341 taattgattg atatcatctg gtcttttact taaagtagaa attctgtgtc tataaacctc
    17401 acttagcgct ttgcttaaac ttccacaatt tgggtattta tctcctaaaa gttttatctg
    17461 caacaaatgt ttttgaagac taagcttaaa ttcccatgta ctattatttt tttctataaa
    17521 acttgagtgt gtaagatccc aataaatttt atccggctta attgcttcta tgattatgtt
    17581 atctgataag aatgttttat ttggatttaa ttttaagttt aaatctgata atacctcaga
    17641 caataatctg ataatcattt cagcatgatc ttttcgaagt gagaagattc tgtagtcatc
    17701 tctgtagcga agtattttaa attcttcaat accgctctct tctattctat tcaataacaa
    17761 tttatcagca taccctagta ctatttcagc tataaaatcc atcaatacac taccttgagg
    17821 tatgccattt gtttgaccat gttgtaaata ttgtatttta gaatctatta agtttcctac
    17881 gccttgagaa ggttttctat gggttttagc ccattctttt gagtgaattg cccaagggat
    17941 ggtatgagta tatattgagc catagcaatc cgtaatatct gtatgaatac agtagtcata
    18001 ttccaaagcg taattgattt gtgcttgctc aagattctcc caccaattta aaatagtttc
    18061 cgcagtatca ctcttcgaag aaatcgattc cactggaagc gatatacatt caattcgctt
    18121 atcagcttgg aattcttcaa acttattttt gatgtgcctc caattgtttt gttgtgtaat
    18181 tacgtttacc aagtctacat atactactgg atgtaatatt tgaagaggtc tccaagcgta
    18241 atttccatct ttgttagcta atattcgatg attaacgtca actgcttcag atagcgatct
    18301 cttatgtttg actagttcag aaaatggttt gtcttttaat aaatccaccg cttttcctat
    18361 acctactgat aaattgatat aattaggcaa agacgtatta aaaaagcttg ctggctttaa
    18421 taaaaattgt tttgcttctt gattatccat ttctaatatg ctttttctag tactattcaa
    18481 attcaaaatc tcctagataa ataaagttgt aatacaaaag tatactctat tgttacatac
    18541 cttatttctt attatatcag tatttctctg tgacacataa gggtgtaaca taattatttt
    18601 aaacgaaatg actacaagta gtcattgaag caaatctcca taagacattt gtaaaatctt
    18661 ttaaaaagaa gcgtcgaaag aaggaacttt tcttcaacgc ttcttttatt gactcaattt
    18721 tcaatattac ttgaacgaat aatctgattt ctaaactctc gaaaaacttc ttgtggttga
    18781 taatagtctg gatctatttc aaatgtttct gagaataatt cattgaattt ttctactgaa
    18841 ataatgattt taccattctt ctctcgactg ttttctatta tttctgcttc taattcaaat
    18901 aaatctagtg acgagctaaa atcataaatc ctgtcatatt gaataaaaaa attaagaaaa
    18961 agttgctcaa actcttcctt atttatcact aatgtttcaa acggaacaac tttattctcc
    19021 tcagccattc tcccagttcc cccacttcca ttttcaaaaa tcacaaaact gatattacct
    19081 taaaagctac tgacaaaata aaacttataa agtaaatggc aacccaacta ggtaaccatt
    19141 tgctctacgt actacgattt ggtaatcctg catttcattc ggtaattcat aatagtaatt
    19201 tattgtttct gctttgtcac tttcaatata ctctggatta tctaatatca tagcatactc
    19261 ataaagctct tcaccatcct tattttcaat cgaccattgt tgtggacgta ttggtttttc
    19321 tgtatcattt ttaagtgtat aggtcacttg taatacacga tcatgttgtg caaattcatt
    19381 tcttttttcg gtcacttcaa tatttttaaa tgttagttca accccatctg cctgataagt
    19441 ttcacctatt tttaaatctt ttgttacaat ttttattggc ttttcttctg gaaaatgtct
    19501 ggctttaatt gttagataac ttccaaaaag aatcaatcct agtagcacta aaagaattcc
    19561 tttaacgagc cactgaaacg ggaagtaaac tttaaacatc ttttttctcc tccgtttttt
    19621 atttgttttt tagagaatac gataactttt gatcaatgtc atccagcttt tcaattactt
    19681 cattacttat cattgattct agttctgaaa ttttttgttc aaatttaatg ctttcagaat
    19741 cattaaattt attatttgat ggactagcta acactggtaa atttgaaacc ataacacgaa
    19801 acatatttgc cttggaacca aaaactcctt tgttttggtt gtaaaaattg ttgaatattt
    19861 ctttagtttc ttctgaagcc gagactttaa aatatggata gttcattttt tctctctccc
    19921 tgtttactca aaaatccata tcatattgat tttgtcgttt ttgataatca atttctcttg
    19981 ctgtttcttc atactctctc cgagcttttt caatcgtacc atcatcaatt gcacgattaa
    20041 ataaataaag aacacgtttt gtttcttgaa tataacgctt cttaaaatta cgttctaaag
    20101 cgggtttagt atcatgaacg ttaaattggt cataatcagt tgataaactc tctgagatat
    20161 cataaacaac ttttttatac accaaatcta cacatttctt cttatacttt aaaggctcct
    20221 cttccactgc aaatccaaag aattggtctt tattctcacc aaaaacatct aatacaattt
    20281 ctattctgtc ctctatttca aatttatatt tgtcataaaa ttcttcataa tcttttgatg
    20341 atctaagaaa tggtatattc tcattatcta attcaaagcg ttgcgctcgt tcttgccaat
    20401 taattctact atccgctcta ccatttaacg atttaccaag atgtcgaata gcaatttcag
    20461 tgagtacctc ttcagattga atttgctccg cttttttatt ttctgtgaac aaatcatcaa
    20521 gataattcat atcatagttt tgccctaaac gctttccgcc aatctttaag attaacccac
    20581 cttcagaatc taagagtttg tacccaatac gctgattttt ggcaaatcga taggtctcaa
    20641 cagaatgttt tttcttcaat tccgcatgaa agtcatcata atttttcgca tactgagcaa
    20701 cttcagaaac agctagtttt attttttctc gatttgaaat cttattttca gttctaaatt
    20761 cacgttcttt ccaattttca cgatctgcaa ctaaatgatt atacttatac atatcaagtg
    20821 ggctaattcc attatcaata agtatattat cattccaggc ctgcaaaact cgaatatcat
    20881 tctttcgaat atccattttc tttcctgttc gctgatcgtt tggtgaaata ccaaagtgat
    20941 tgtgaacatg ccctttgtct gtgtgtgtga cgaccaaaat tgactggtta ggaaacattt
    21001 tttctgccca cttaacaccc aactcatgtg ctttttcagc ggaaatatct tctagtggat
    21061 caaagctttg tgtaaaatgc caaagcttta ttttcccttt atcaccaaaa atttcattgg
    21121 ttcgtttaaa atcctcaata gcatttaaat cattcgatac gccaattcct gtcacgagtt
    21181 cagaatcttc ttgtttattt aacatatatg aaattgtatt atgaatactc ctactttttg
    21241 aagcaatttt tataatcgag cccataactt attcaactct ttttccaagc gattcaatgg
    21301 atcacttatt gattttattt gtatcattaa gtcatttttt ttagcatata gccgctttaa
    21361 gcgctcttgc ttagttgtaa aagataagta aacactctga tgttcttctt tttgtatctc
    21421 ggtatctttt ttatttaact gaacacgcaa ataatttgct gttttcacta attgattcaa
    21481 atttcttcct attgctagta aatgaaaatt aatttcttta tattgtcgat cggtatattt
    21541 tagacttttc cctgcgttgt ctactgcgat tttcttcacg tacattggaa cagataaacc
    21601 aaaaatgtct gctgtttttt ctagctcttg gtactgataa tcgtctactc gaaactttat
    21661 cggcctattt tgtcgtcgac tatcttcatt acgtttatat cgctttcgcc tttcttctaa
    21721 tggtagttta ctgacatcaa ttgccatgcg cacttctcct ttccaatatt atttttaagt
    21781 tcaaaagaag caagcgaggg gaaagggaac ccttccccgc ttgtcgaggg agtaaactcc
    21841 ctacgagcac cctcatgctc cgcattttat cgagattaga tagtcgataa atcagctacc
    21901 taacactttc ttcttgcgtc tcttctcgat agaaaattcg atacgctgtc tctccttttc
    21961 ttctcaaaaa ttgttggaac ttcgtttcca acaattgatc gcttgcaaga aattcatgca
    22021 ctagggtaaa ttcgctattc gcgaaattta ccgccttttg ccatttttct cgagtacctt
    22081 tttgattaat ctcaaaagtt tcttcaaact cttttactga tgaaattcct gttttttcat
    22141 aggcaaactt tcccagttcg ggataaagct ttctctccaa ctcggcgagt tcttttttca
    22201 aatctgcaat tgatttttgt tttttccttg ccatttttca tgctcctttt tgtatgtgac
    22261 taagttcatt cataagctca tttttcatct ttaattcttc ttgaatcatc agttcaatat
    22321 aattagtgac cgtgattttt tcatttgttg ctttctttac tgattcacat aattcgtcaa
    22381 gttctaagaa atcatgtgta ctaatctgta gaattaattg catgaaaatc acctctttat
    22441 tttttaagta aactatcgaa aagtggatta ggatcattaa gtgtatctct gaataactcg
    22501 ttgtcttcat aggtaaatct tcgtcttggt tttgatccct tatcattttc acggatttca
    22561 ctatcaataa tcactgtttc atgatttgaa tcaaattttt tcgctgactt tctgtcttca
    22621 acgatattga ttttttgtgt tttttctttc atttctccac tatctttttg agcgttctcc
    22681 cgaacatcta ctgttttgaa cccttgacta cttgtagaac tttgtacatt ttctcggaca
    22741 tctactgttt ttggttgact tgcactgcta ctttgcacgt tctctcggac atccactgat
    22801 ttaaagcctt gactacttgt agaattttgt acattttctc gaacatctac tgtttttggt
    22861 tgacttgtac tgctactttg cagattttct cgaacatcaa ttgtcttagt accttgacca
    22921 tttgaagaac tttgtaaatt ctctcttaca tcaacattcc gtccgacatg accattcaat
    22981 gaaccttgaa tattttctcg gacatctact gatcggccac cttgactact tgtagaattt
    23041 tgtacatttt ctttgaccaa tttttctaat ccggtatttg aatttttagc ttcttggatt
    23101 ctttctttgg tcaacgcttc tttttcgtcc atattagcgc ctgtacgccc ttctacacgt
    23161 ttccttaaca tttcctcacg attcattgct tcaggcgtgt atttcgctcc ctgtcgcttc
    23221 tgattaatta attcttcagc agacttagct tgaccgtgca tagcattttt acgttcttca
    23281 atatcttgtt gaactttttg ctgaattgca cttacaggat tattattcga ttcagcatta
    23341 tttttcattg cttcacgcat acgttcagaa ctagacataa ttctatcggc attttcaacc
    23401 ttagcaccat cttttctatc cgaaagacgt gttggcatgg aatcagcata ttttccagtt
    23461 tcatcttctt tatttttagc taacataggt ttgctatttt tattaatagc ggacacggta
    23521 ccatcatcta aaccttcttt gaacttattc ttggtaccat tagccatatc acgcatactc
    23581 ttaaatggtt ttattgctct ttcactagcc attgtagctc catctgaagc taaacctgat
    23641 aatccacgtt catgggcata tcccaaagct cttccagttt tgccagcttt ttttgataga
    23701 gtttcgccga aattcttttc tggttttcga tcttctcctt ctccgctgtc tgatccattt
    23761 ccatttccgc ttcctttagc acgattaaat ccatttgatc cttttctaaa cagcattgcg
    23821 cccataccaa aagcagaagc aagttgccca taaccttctt tgagtccaat atcaacacca
    23881 aagtatctca tgactgttcc actgcctttg attaaaacaa aaacggcaca gatataagca
    23941 attgttttta taaaaatatt tgttgagata ccttgtcctg agttcagaaa attgataaac
    24001 atagcaaaca tggatagtcc aaatccttga aagcctacag tcaaatagca ctgtagaata
    24061 tctgatagaa caacttttga acgctgtcct gtttctaagt cagtagcaaa tactaaaact
    24121 ccgagtacac gtttaaatgc taactcgagt attgcagtaa taatcacaaa ggcgctaaaa
    24181 acataggcta ttgctaaagc tatcagccca attgaaatgc cccatagatt cgcttgatag
    24241 cgataatatc ccgatttcaa tccatctgta aatgtagata aaaagttatc ctcaaatttt
    24301 gaagcaacaa attcattatc ggaattttca actaattcat aacgcaaatt atcagcagtt
    24361 gcgttttctt tttccatttt ttcaagtaca ttatctgtca atacttgtgt aagatccgtt
    24421 ttccaaaaat ttttctttgt taaattgttc cgatcattcg ttttatcaat cattgagtac
    24481 tgatctgttt tattgatata agctaaatct gtcactccct gttcaatcag tgaccacgcc
    24541 acaccttcgt ctgagtttgt aatcgtagtc gtatcattat aaaaagtttt agaaaactct
    24601 attccactgt gaattaaagt tggcattaaa agaatcaacg caatcgacat aacaacgttc
    24661 attccaacgg atttcaaatc caaattccct ttgccaatca ccatcttata tccgataatc
    24721 atcagtgaaa gtatcattaa accaataaca actgtactta agaccgattg ataaacttta
    24781 ttcattcccg tattttcaat aaagctaaac aaatctaaca cttcgggaat catattttga
    24841 ataccatctg tgattgcaaa cattgctttg atgactcccc agcctatcca catgccgaac
    24901 tgtccgtaaa agtctccatt gaattttttc aagtacggtt gataaagagg attattcaaa
    24961 aattcagttt gactttctgt gtcatcaaac caattgaaaa tgcttgcttg aacaacatta
    25021 ccggtgaaaa caacgataaa cattaaaaca agtatgctca aagctaacca gtgtattttt
    25081 ctactggcaa tcttttcttt aactattttc attattacct ccctcagccc attttttgag
    25141 actctccgag agcttctgag ccatttcttg attaattatg atatgagtta cacaattgtc
    25201 attttcagct cttaaatcga gaaccacggt gttaaagcag atactctcga ttaattcgag
    25261 agtatctaca ttttgatcga gacagcagat ttggtctatt agacgatttt caatcatata
    25321 acacctctct tgtttgagta acaaagttat tatatgctct ggaacttaca gtatttttgc
    25381 agttatataa agttgtaatc cagtaaagat tgagattgtg tatctcatga tcaattgcgt
    25441 taaatgaatc aatagcagat tcaatatcaa attgattgag ttgttccact ttttctatca
    25501 cttcatttgc agatttttct tctctattta tcctgtatgt tttgctcttt tcagagtata
    25561 ttgattgaat tatattaaca atatcttcaa caattctttt gtaacaagga ttggatttta
    25621 gcgcattata tccaatttga ttctttattt tttgtctcat aatttcgact tccatgtcat
    25681 ccgatagatt gatagattga tcttccctaa tctcacctaa actaacctta cctgacctac
    25741 tctgatctga actaacctga cctaatctaa cctgggcatg cccatggtcg tcctttggta
    25801 gaccaacgga ataccatttt tcaatcaaat catttcgaag catttccgtt tgacctgctt
    25861 ttaaaatttt ttcaatacta atttgtttta atcttgtaaa aaagtctgga ttactattta
    25921 ataatttaaa tcgatatata gagggtgtat atcttgtagt tcttaacgtt tgattttgca
    25981 gccaatgggg aagaaatact aataaatctt cattcagcat atagagaaat tctttagcta
    26041 aaagaatttt cacgtcatca tcaggggttc caagcatccg tacatatgga tatgcttcga
    26101 caatcccatc atcgtctgcg tttacattca gatatacata taataatttt gctgaatccg
    26161 acatcttcat aaaactcgca ttatttaaaa aagtttttga caacatacgt ttttctgcca
    26221 taaattccac acttcctcct gtcaatgtta aaatgtagtc aaataaagtt ttcacagaac
    26281 tttattattg agtgttcttc cggtctgcaa actgtaagga gcactctttt tttgtatatt
    26341 caccttttat taaatcaaat agaaaataaa tcttgatttc ttttaaactc aactttgctt
    26401 cttcatcatc gttcaaacaa acacttcctt tttttcatat caaaagcaca ccacgcaact
    26461 ttttttatat ttcatattct ttgataaatc ttgctaactc ttcttttctg ataaattgac
    26521 agtctccgat gatactcacc tttaaaccac gctgattaat gaattttttt agtgtacttc
    26581 tactacaact caaatattca cacgcttctc caagccggaa aatttctttg cttaaagatg
    26641 tctcaatacg aacttgatca acagcttcct tagcaacttg ataaaaatat ttacgtaatg
    26701 attgatcaag atctggcggt agaataactt gtacactcga tacatccatg atgcccactc
    26761 ctcattcgtc ttttttcgtc ttcaaatcac taaaaaaaat atccacaata tctaaatgaa
    26821 ttccatcttt atgaaatagc tttacgattc tttctatttc ataatcttta aacggcaccc
    26881 ttcctttctc tttgttacga tacgttagtt ccgatatttc taataactcc gcaagttctt
    26941 tttgtctata tccacagaac ctgcggtatt ttctaactgg agagtccaac cttaccacct
    27001 cgtctttttt cgtctttatc aacgatagaa tagcatttat ttcattgtcc gtcaatactt
    27061 acccgtcgtt tttcgtagat tccattccgt ttttgtgtta cattactgtc ggagggatat
    27121 aatatgaacc ttaataagac atcagtagga aaccgtattc gtttagttcg taaagaactc
    27181 gggctttcca tgaaagagtt cggaaaaaag ataactccta ccgccgcaga tagtattgtt
    27241 tcgcgttggg agcgagggat tagcttacct aataacgaca ggctaactca aattgctaaa
    27301 attgctcata aagatattaa ctggttatta tggggaactt ttaaagaatt tatttcatca
    27361 ttgttagata aagaaggata cacaaatttt ttgaatgatt ttcctgaaac agtagatact
    27421 atttataatg atttaatgcc tatttcggaa aatttacagt ttcctgatta cgaaaattct
    27481 attttacgac tattcaataa gatatactta cctatattca atgattactg tctagacatt
    27541 attgaactgg atttggagga ctttataaat aagagacatt ccatctcctc tgacaagttt
    27601 aaaaataggt ttttatcctc ctttcatgaa ggagtatcta acagcggata taaatacggc
    27661 gatacggatt taatttataa atatggaatt gcattacttg agaaatttga cgattcttat
    27721 aaattaaata atccatatga gtcaatagat gcgtattttt ctgccatgac tgtaaacact
    27781 gaagataccg aaaaacttat atataccatt tgtaaatcgc ttggcattcc attccaaaaa
    27841 ggaagcaata ccgcacagat tctttcaaaa aaccacaata atttttaatt cattttttct
    27901 atcaaaagca cgccacgcaa ctgataggag gaaaaacaat ggcaacaatt aagcaatatg
    27961 aaaagaaaga cggaaccaaa gcttggctat ttcagaccta ccttggaatc agtccattaa
    28021 ctggtaaaga agtccgtact acacgtagaa attttaaaac aaaaaaagaa gctcaaatcg
    28081 agcttaatag actacttgta gactttgaag agaacggact taaaaaggaa acaaacgaaa
    28141 ctttcaaaga agtgtatgat ctctggtatg agagctataa aactactgta aaggaagtca
    28201 ctcaattaaa aattgagatt aagtttaata aatggattct tccaaaatat gggcagttta
    28261 gaattaaaga aattactaca aaacatgttc agaagatatt gaatcagtgg gctaaaacaa
    28321 cagatcaata taaagtccta cattctactg caagccgtat tttcaaatat gcaatgactt
    28381 tagggataat tactaaaaac ccctgtgaac ttgtaatcat gcccaataga aacgttgaaa
    28441 aaaagaaaga tactgtcaaa atttattcac gggatcaact agaaagtctc ttccaatact
    28501 tggaatcaaa aaaaagcacc tatagagccg actacgataa aacgctactt cgctttctat
    28561 tttttagtgg ttgtcgaatt agcgaagctt tagctttaga ttggtcagat attgacttta
    28621 attcaaacac cgtcactata aacaaaacct taagccaaac aaaatatggg tacaaaattt
    28681 caagtccaaa aacgagaaat agctatggtt ctctatcttt agacaataaa accattaatt
    28741 ggctaaagaa atggcaaatc aatcaaaaaa gatatatgtt aacccttggt attactgatc
    28801 caacaatgat tttttgtgga atctacaagg aaatcattac taaacacgct atttattctc
    28861 gtatgataac aatctgcaag aattctggta ttccatttct aggtaaccat gtaaccagac
    28921 acactcatgc tagtatgctg ttagagtcag gagtctccat gaaggaagtt caagaacgcc
    28981 ttagacactc atcaatcaaa ttaacaatgg atacctatgc tcatctaact aaagaaacca
    29041 aagaaaaaac cgttgaaaag cttgcagagc acctaaattt ctagcggaac gtattcaatc
    29101 tcgtattcaa ttcaaaatac aaaaaagctg aacccttatc taccaagggt tcagctacca
    29161 tttaaagctt attcttcttc ttccgccatg aatgtattga atacttcttc aatcatgtcc
    29221 cattctgcgt ctgtttcgat ttgttctaac tcgccttctg ttccatcttc gttttcgatg
    29281 tatgcataag cttgtaattc tacatcttca tcttctgaaa caccagcagg atataaaaga
    29341 acatagttac gtccaaattc ttcttgtccg tcaactgtta gtaggatttc atataatgtt
    29401 tcatttcctt ggtcatccac caatgtgatg tgttcgtgtc cttcatggtc gtgggaatgg
    29461 ttatgttctg tcataagatt acctcttttc ttttattatt aaaataattg cttacctgag
    29521 ccgtctaaat aattttgtaa gatcatcaca gctgctaatt tatcgatgac tttttttcgt
    29581 ttggctcttg aagtatttgc ctgctctact agcatacgtt ctgcctgaac agtggttaat
    29641 cgttcatctt gataatcaac tggcaaggag aatagttctt tgatcttttc tccgtatgac
    29701 atggaagctt ctgcccttgg tccgatggaa ttgttcatat ttttaggtaa tccaactaca
    29761 aaccggtcta cttcatattg tgctactaat tccttcaacc gatcaaagcc aaactcttct
    29821 tctgcttcat tgatccgaat gatctctact ccttgagctg tccagccaaa ggggtcactg
    29881 atagcaacac ctactgtctt agaaccgaca tccaagccca ttactctcat aaattcactc
    29941 catgatttga gaggtagtcc ttgacaatta cttccatgat ctcatcgcgt tcgtgacggc
    30001 gaatcaaatt tcgagcatca cgataacgag gaatgtaggc cgggtctcca gaaagcaagt
    30061 agccgacaat ctgattgatt ggattgtagc ctttttcttc caaagcccga taaacaatcg
    30121 ctaatgtttc gctcacttct tttttgcgac tatcgtcaaa accaaaacgt acggtttcat
    30181 ctgtaaaacc catgattcta cacctctttc ccgcaaggta attccagctt accttctaca
    30241 accttatttt actcgaagaa agggaaaact acaaacaaat attccaaact tccgaataaa
    30301 ttaagaaaca ctatttgtat gtttttttcc ttttttctgt agctttatca aaagtactct
    30361 tttcataaag tatacgctat atttctttga atgtatgttt ttacacaatt ttttcataat
    30421 attcataaaa aattcgaata gctgctacta attttttgtt tttgttgctt gtcctgtctc
    30481 ataattgatc gtgtagccat cttgaacatt gtagataaag acattgaatt caattttttt
    30541 gtcttcaatg ctttctgctt ccaattgcac tcccctggct accagttcat tcccctcaaa
    30601 atgaggagtg acacggtagc gtacatgatg atttgtttct ttgatatatg ccgcaatatc
    30661 attttcgtga gcaagcatgt atggagcatt caacgaacgt gtccctgtca tcaagttttt
    30721 aatgttgtta ttttgaccag tcagctggaa accaattaaa tggctgcgat tatataacca
    30781 ttgcccgtca cttaatttct tttgtttcca acctgtcggg tcaatataca aaggctctct
    30841 cttttcagta gggaacaaat cttttccaag catagcattg gcttgtccta ctctgttcaa
    30901 tttatcaata tttgaatagc tttcccatgt tcctttggaa aggctaagat cctcttttgt
    30961 aaattccggc tgattcccat tgatttctat ttcttgatgt tttccatcat attctggcac
    31021 atccgctact tgagaagctt gtggtgtgga atcaaaaata gttgtaatct gctgaaagat
    31081 atctgaatct ttttgtgtgg tggaaacacc gaatgcccct gcaatcacag cagcgacgat
    31141 ccccacgatc gccttggttg tttttttgct catttcttta ctcctttact aacagctccc
    31201 taaatcttaa catatgttcc cctataaata aatactagaa agaaatagaa acagcaaaaa
    31261 ataaaacctg ttttaagatg gcttaaaaat acaaaaaaga gacggattcc tcgtctctaa
    31321 tcagtcacca ctctatctgt aaggtattta ttattattca aaaattttct tcatactcaa
    31381 gacaatacac tgatttcaca accgcttggt aaacggtgcg caaaagcttg catctgcggt
    31441 aataagtcca tccaagttaa aaacataaga agttgttttt gcttggctgt tcttattact
    31501 tgttgcagag cagctttttc acaacctatt ttaagcgggt gacgggaatc gaacccgcga
    31561 catcagcttg ggaagctggg attttaccac taaactacac ccgcaataca aaaacagtat
    31621 acgcctcttt tattccttcc tcaagttttt ttattcttta cgcttctggt atgttttgta
    31681 gtaaaataaa actaatcaat caattattgg agaagatgct atgacgggaa aagaattaga
    31741 tctgttgctg gacacttgtt tactcgctgg gaaaatcatg atggaaagca atgcggagat
    31801 gtatcgggta gaagatacga tgagtcgaat tgctttagct tctggaaatt tccgtttggt
    31861 cagctatgtc actcaaaccg gtctctttat tggtcttgat cgtacctcga ccatccgaat
    31921 ggaacaaatt accaaccgtt cgattaattt agaaaaagta gtaaaagtaa atgatctttc
    31981 acgtaaatat gttgcttcag agctaacatt agaagaactt tactgcgaac tgcaagcaat
    32041 cgaccgagac cgttctttct ttcctgtctg gttgcaaatc gttagcgcgg cagtgatcag
    32101 tggaacaatt atggtgttat ttggcggaaa gatgaccgat ttacctgtga ctttagtcat
    32161 tggaggattg ggatacgcag cttatctata tagtttgaaa ttttttagaa tcaaattttt
    32221 atcggaattt ttatcttctt tattgattgg catagccgct atctacagcg tacgttttgg
    32281 cttaggaagc aatcaggatc taatcattat cggatgtgtg atgcctttag ttcctggagt
    32341 tcaaattacg aacgctatcc gtgatttgct agctggacat tatgtctcag gagtttctcg
    32401 cggaacagaa gcgatgatga ctgctacgat gatcggtttt gcgattgctt ttattttcca
    32461 gcttttttat taggaaagga acacaagtat ggggacgata tttattcagt tttcttttag
    32521 ttttttagcg actgcggcat ttgccgttat tacaaatgta cctaggcgtt cattgatttg
    32581 ttgtggtctt tctggtgctt ttggctggat gatctactgg atctgtgttc aaataggcgg
    32641 atctgctgct tttggttcat tgcttggttc tttaggagta gcattcatca gcgatctttt
    32701 ctctaaacgt ttgaaaatgc ccgtaacgat tttcaatatt cccgggatgg ttcctctagt
    32761 ccctggtggt ttagcttatc aagcggttcg aaatctcgtt actgaatcct atcgagaagc
    32821 ggctcattat gcggtccaag caattatgat tgctggagca atcgctttag gtcttgtatt
    32881 atcagaagtc tttaaccata acatccgtaa tttcaaagta aagcgtgacg caatcatgat
    32941 gaaacgaaaa aaacggcttt ttaaataaac gaagtgtaac aaagaaaagg ttgtgaaaga
    33001 gacgttcagc cttgagcgat gacaaaatat ctcacaaaca ttgcgacaag taaccgcagg
    33061 agcaataaga agtaattatg agtattaaaa aaagttttgt aaaaatagct tctcacattt
    33121 ttacataaaa ttgacttaat actgagaaac tgacttttga acaccctcta tctgtaaaaa
    33181 aggggctgcg acatgatttt tgtcacggcc cctcttcttt attcaaattt tttccaaaac
    33241 gttcgttgat aataaacgat ccatagagcg gaaagtatta gaactaaacc gatcacagga
    33301 atattcacta accaaaatgg ataaaacaaa gccaacattc cgtataagac gataagaata
    33361 atactgatga tcaatgaacc catcatagtt gcgactaaac ccaaacttcc tgaacctctg
    33421 ttaaataatt ggttaatatt ggtccagttc agcagcagca accgataatc tctagcgaaa
    33481 taacgtagtg acaataagta gcagccgaat aaagaaccaa atagcaagct agggataaaa
    33541 aacaaaggca gctgaaaaga cagtccgctt aataatgcga tcccaccagt taaaaaactc
    33601 tgcaatatcc atccgaatct gaacttttct tttaaatatt ggttcataga gattggcaaa
    33661 gaacggataa acagaaaatt ttcttgatcc aaagaaataa gattgctgat aaaagaagtc
    33721 tgattaactg ttaagaaagc caatgcgatc cctaccagga aaacaacacc gattaactta
    33781 ttatctaaat cagttaattt tatttcgcct cctagggcaa aagcaagaat aaaaatgatt
    33841 ggagttaaca aggaagaaga aagaacttgc ataatcaaat taggttcttt tactaattga
    33901 gcattgtaac taaataaaag ctgatgcaca ttttggttct ttttatgggt tctttttatt
    33961 ttgaactcac tgtttgaatc agtcagttgc tctgtaagct ttggtactat cagctgtttt
    34021 acagcaagga aaaatcccag agccgtgata gctagtattc cccaacccaa taaaccttgt
    34081 tgagaaagtg gctgactggc agcatcatag aatggcaata atatcgaaat cggatgccga
    34141 tctgttaact cattttcaat agtccccgtt tgatgattca tccacatgat tccagcaaat
    34201 gcgatcagca tcgagctgcc aagcaagata ctagaaactg tttttctatg ttttttaaac
    34261 actgctgttc gagccaaacc aaaaacaatc aaactagcca gacaaaaaag aataatcaaa
    34321 aatagagaaa acaaaaggat tgagataatg atagtaagca aaatccctag atcagctcgc
    34381 catccggtta aaataaacat gactagaagc ggaaaaacaa aaggaatgat ggtcaaagca
    34441 actaccaaaa tctttgctga aaaaacctgt tcttgtccaa aaggcagagg caaaaatact
    34501 tgcaggtcat ttccttcaaa aaagatatta taaatgactg atatactttg agttaaacct
    34561 aacataccaa aaagtgccac gtaataagta aagaaacctg gcaatttaac aaagttcata
    34621 gcaaacatag caaatccgta aatcagtaaa aaaatcagcc ctgaaaaaag attttgagac
    34681 aaaagataga tagttaatcg ctttcccttt tttcctttct ctcttgcttt attcgtgagc
    34741 tgtggattaa catataaaag attaactcga attaattcta ataattgtcg cttatacata
    34801 cggtccatct cctgtcgctt gccttccagc cattttcaaa taaatctttt ctagcgtttc
    34861 atcaggtgag ccattcaata attcactaac tgccccctga taaatcaatt cacctttctt
    34921 caaaatagcc agtttatcgc ataactgttg tgcggtatcc aatacatgtg tagaaaaaat
    34981 gactgttttt ccttttgcgg catgagcctt catcatttct ttcaaatcaa atgctgcttg
    35041 aggatcaagt ccttgcaaag gttcatccaa tacccaaata tctggatctg gcagcaacgt
    35101 gccgatcaaa atcgtttttt gacgcattcc atgtgaaaaa cttgcgattg tttcattttg
    35161 atgactatac atatcaaaaa gcgctgtcaa ttctgctaaa cgcttttctt ttttttgtgt
    35221 gtctaattca taggcagctg aaatcaagtc ccaatattct ccagccgtca attgtaaaaa
    35281 aatatctggg gaatcaggca cataaccgat tttttgtttg atactcaatc gattttccgt
    35341 caatttcatt ccatctacta aaatcgttcc cgatgatggt tcaataatac taactaaact
    35401 tttaatcgta gttgattttc cggcaccatt atgtcctaaa aatccaaaaa tttccccttg
    35461 tcttatcgag agagacaagt cttttaatgc ttgttttttc ccgtaacttt tacttacatt
    35521 gatgaattca atcatttctg tactcctttt gtttgttatt actttttatt gtacccttcg
    35581 ttattggtgg agtaaatgtc aaaaagagtg cataacaaaa aaggatagaa acgactaggc
    35641 tgctacttgg gttgttctct tctacaggaa aacactcttc taacaggtaa gatatgagtc
    35701 tttgttttct ttaaaatgat tcattttcta tcttttcttg cataaaattc tttagtttat
    35761 caatacttct ctttcccatc tctagttgtt ctccatttgc aaatgttact atttcgttta
    35821 ctctgctaat agatgtaatg ttttcagtat taatgatata agcttttaaa tctttacata
    35881 aatactcata ttcgagtgat tttttatatt ttttaattgt gccgtttacg agaatctctt
    35941 catctttcaa tactaggcct attttatttt tataacctgg catgctcatt agataaagga
    36001 tttcatcgta atggatatag tgatcgctat taccataacg gacagttgtc cacctttttt
    36061 tcgtagcatg ttgaacagga tagttttctt tttctttcaa taagtaactt tggatgcttt
    36121 ttttattttt ttcataagtg aattctttta tcaaataacc actaggcgta atctttcgat
    36181 taataacagt tattgcttta ttcggatgag acgttagata aacaatttta cacttttcat
    36241 tcttttctct aagcatttcg cctagttcga taccgctata ataggcgttc aaatcgatgt
    36301 ctaggaaaaa aatatctgag gaatagattt cattattttt aatgaattca acaaattgta
    36361 taacgttcaa tccctgaacg atctcaaatt tccaaggact taataaaatt ctggattctt
    36421 ccaaaatttc tactagcatt ttttcaaaaa ccaaactatc atccaaaata aatatacgcg
    36481 ttaccataaa aagtacctcc actcgatatt ttactatcaa aatcatttca tacaactttt
    36541 caacatacat gttaggaata aattgtaatt ctcttcacaa tcttttccaa cacctttgaa
    36601 accgatatca aatactttca ggatgctgct tctaaaataa atatcaaaca aatgaataca
    36661 tcatgcaaaa aaagccagac aaccgacgtc cttgcgtgtc agctgtctga gctatgttta
    36721 tttattttgt tcttttaaaa agaacgatac acatactgat ttttcggagc agagactttc
    36781 tcagcgtttg taacaactac agatgggatt tcacgcttga atggttgata atagtctgat
    36841 tggatggttc cttctatttt ataccattca tcatttttaa ccgttactcc ttccggcata
    36901 tgcgtcaaca aaccaaagac tcctgaatct gccacacaat ggataatgcc gaatcggaac
    36961 aagaagatat ctgtattgct atcgttcggt gtttggtaaa caaatccttc atagctgatt
    37021 tttttgccaa tgaattcgct agggtaatta taaattagct ccataacttc taaatagttt
    37081 tcatctgtga tcttgatagt ttgcttatcc ccatactttt ctttcaactc attcatttgg
    37141 tctaagtagt ctgatttgtt gaaataaata ctggtatccg gctttaagta ttgtgtctgc
    37201 atctccggat cccctactga ttctttactt actgggaaat taaatccttt ggcttctacg
    37261 atcgtagtgt cgagactaac tgttggaaac aacgtcccca cgattactgg caaagctagc
    37321 agtacataag cgattccacg ttgataaggt tttgacaacc catggtcatg atcgtgctcg
    37381 tgtgcctttt cttctgcttt tccgtttttg acccacaaat acagctgaac taaagccaaa
    37441 agagcagata aaatcattga aagaatagct aagtagcgat aatggacatt gatatattga
    37501 tcgagtttgc cagaaacttg caagtacatc atcaattcga aatacccaga taaaatcaaa
    37561 aaccgcagca tattttccct cctaaataaa taatgtgaat ccaataacgc taagggcaat
    37621 cagtccgatc agcgataaaa taaagcgtcc tgtaaaatat cgtttcatca tcagtagatt
    37681 tttgatatcg accattgggc caaagaccaa aaaggcagct acaggtgctg tgccgaacag
    37741 acttaacaaa gaagatccta taaacgcatc tgcttctgag caaagagaca tcgtaaaggc
    37801 caataacagc atgatgataa tggctaaaat ctttgttgag ccgagcgtta gcataattct
    37861 agttggtaaa taagtctgca tcgcagctgc gattaatccg ccaatgatca aataacgacc
    37921 tgtatcaaaa aattcgtcga ttgaatgtgt caacacatgc ccagtttgat ggatcagctg
    37981 attttttggt tcattcttct cactttgttc ttcttcatgg acatgatgat ggtgattttc
    38041 tgctacttta gacgtgattt tttctttcaa tacagcgtct ttttgaaaat aggcaagcca
    38101 gacaccaatc acaagggcaa caaaaaagct gcctatcacc cgccagcttg ccatctgcca
    38161 agaattgcca aatgcgataa aagtggaaaa aatcacgatt ggattgatga tcggagcagt
    38221 cagcataaaa gcaaacgctg tgtgaactgg tacgcctttt ttaacaaatt gatgaacgat
    38281 cggtacgatg ccgcattcac atgaaggaaa gaaaaatcct aagatcgaac cagtaaaaat
    38341 agagaagata cgattttcag gaagccatct cttcacacgt tctggggtca aaaatacttg
    38401 taaggcgcct gagataagac agcctaataa tacgaaaggt aatgcttcaa taacgatcga
    38461 taagaaaatc gtactcatct gcaaaaccga atttggaata aaagaaaaca tgagtttcct
    38521 cctttttatt tctagttcat tcgctttgca aatatacgct tttattcaaa aaaaacaagt
    38581 tgctctgtaa atttttacac aactcttatt ttttaccaaa gcacttcaat tgactttttc
    38641 tgttattccc attaaattaa ggtctcatac atatcacttc tatcataaaa gggagaataa
    38701 aaatgaaaca aaccactaac ttgactgaag ttcaagacat actgcaacag tcatccgttg
    38761 ccgtcattta tctttcgatg cctaattgca gtgtttgtca cgctgttcgt ccacgactag
    38821 aggaacttct cacacattat gatattccgg cacttcattt ggatgcattt gaaacaccag
    38881 aagtagctag taaatttgaa gtgctgactg cgccagttgt cctgattttt catcaaggaa
    38941 aagaagtctt tcggcaagca cggttcatcg actttaaaaa aatccgttat ctactagatg
    39001 aactgacagc agaagatgat tccctttctt atgaagaaat cttccgaaca aaataatcgt
    39061 tgtccaaaca gaaaaaagcg attgaacaag tgaaagtttt gccttcattt gttcaatcgc
    39121 tttttatttt tattctgctg tattttcgga atgcgcattt gcgtcagaat gcaagaaata
    39181 gatcaaggtt tgcaattcat tcgtcaaatc aacattttgg atcaatacat ctgacggagc
    39241 attcaatcga gcagcggtaa agttcaagat tcctttgatt cctgcttccg ttaactgatt
    39301 gactacttct tgtgctcttt cagcgggtat cgttaaaatt gctacttcga tttgttggat
    39361 cttgatttgt tctgacatat cttccattgg ataaacgggg ataccgtcta cgatgcgccc
    39421 aacgatttcc tcattcacat caaaagcaca actgatacgt atgctattac tttgatggaa
    39481 tttgtatttt agtaaagcac ttcccaagtt ccctacaccg atcagcgcaa catttgtcaa
    39541 ttgatcgtcg tttaatgtct ttgcaaaaaa gtgcataaga tcttctacat cataaccata
    39601 cccacgtttt cctaattcgc caaaataaga aaaatctcga cggatcgttg cactatctac
    39661 ttgaacggct tcacttaatt cagttgaaga cacttttttc tttccagcat tatttaaaat
    39721 ccttaaatag cgataataca aaggcagtct tttagctgtc gcctttggaa taatatgatc
    39781 tttcacgaaa tgacctccac gtattgtcta attacatctt tgatttctcc atcatagcag
    39841 tatttatagg aaaaaatcaa ttccattgct cagatattgt gaaattttat tctttattca
    39901 tttatttatc atttctgcaa atctcgcagt ataatggaat ttatggtaga ctactaattg
    39961 aaaaatagtt ttttatttga ggagaattca caaagtagaa aagaggattc catgatatta
    40021 ttacaagcca atcagattgc tcgccatttt ggtgcagaag tcttatttga aaacatacat
    40081 ttagaaatca gtacaggcgc tcgtgtcgct ttagttggca gaaatggcgc tggaaaatcg
    40141 actttattaa agatcattgc tggaattgaa gcacctgatg aaggaaagat cgctaaaaat
    40201 aaaacagcga ctctcggata cctcgctcag gacacaggac ttgattccaa tgaaacagtt
    40261 tggaacgaga tgctgaaagc ttttgaacaa gttagacaaa tggaagagcg gatgcgcgag
    40321 ctagagattt tcattagcga aggtacgcca gacacacctg cttatagttc ttatttaaaa
    40381 gaatacgacc ggctgcaaca cgatttttca gatcgaaacg gctatggtta tgaaaatgaa
    40441 attcgttccg tattgcatgg gtttaaattc gattcttcat tttatgatca gaaaatcgat
    40501 actttatcag gcggacagcg cacacgttta gctttagctc gcatgctttt gcaacaccct
    40561 gacattttga ttctagatga accaacgaac catttagata tcgagacact ttcctggctt
    40621 gagaattatc tgcaaagcta tactggtgca ttattgattg tttcccatga ccgttatttt
    40681 ttagataaag tagtcaatga agtctatgaa ttaagcagaa gaaaaatgcg ccattacaaa
    40741 ggtaactatt cgaaatatct agatttgaaa gcagaacagt tagcaagtga atggaaggct
    40801 tatgaaaagc agcaaacaga aatccataaa ttagaagact tcgttgctcg gaaccttgtt
    40861 cgcgcctcta ccacaaaacg agcgcaaagc cgcagaaaga cattagaaaa aatggaccgg
    40921 ctggatcgtc ctcaagggga tgaaaaacaa gcaaactttc tattcagtat agaaaagcct
    40981 tctggaaatg tagtcttgca aatcgaagat gccgctatcg gatatgataa tgtcacctta
    41041 tctgaaccaa tcaatctaga tatccgtcgt caagaagcga ttgctctagt aggtccgaat
    41101 ggtatcggga aatcgacgtt gctgaaatca atcattcacc agatcccttt tatccgtgga
    41161 aaagaaacgc tagggacaaa tgtcaccatc ggctattacg atcaaggtca agctgatctg
    41221 catacaaaca agacgatcct agcagaattg tgggatgagc atcctaccac tccagaaaaa
    41281 gagatccgaa gtgctttagg tgctttctta ttttctggcg aagatgtaga aaaaccaatc
    41341 cctctgctaa gtggcggtga aaaagcacgg gttgctttag ccaagttagc gatgaacaaa
    41401 gaaaattttt tgattttgga tgaaccaaca aaccatttag atattgacaa caaagaagta
    41461 ttagaaaatg cattgatcga ttatgacggc acactgcttt ttgtttccca tgaccgatat
    41521 ttcatcaatc ggatcgcgac aaaagtcgtc gaactctctg aaaatggcag caaagtctat
    41581 ctaggagact atgattatta tttagagaaa aagcaggaag aagaagaaat cgccgaattg
    41641 ttggcacaag atgagccaga atctgctcct gctcccacga acaaatctga ttattaccaa
    41701 agcaaagaac agcaaaagct tatccgtagc cttcaacgaa aaattactca ggttgaagaa
    41761 gaaatggcgc ggatcgatga atggatcgat cagttggaac aagaaatggt cgcgccagaa
    41821 aacctggaag atcacgtaaa actcaatgag ctgaaccaag atttagaagc tgctagacaa
    41881 gatcaagaaa caaaattgac ggaatgggaa gaactaagct tggaattaga agaatatgaa
    41941 aattaacgaa aaaaccaaaa cagaaaccat tcatgctgtt ttggtttttc tttttttatt
    42001 ttatcaaaca aaaattccat atttaccttt ttcaccaata agaagaccaa ttactagtca
    42061 ttctcactaa agcttctaaa taatagtaat caccccaaat acaaggtgta tttaccccat
    42121 tatttttggg cttattataa acagactgtg ttaaaaagcc atcttcttct tccgttgtag
    42181 catatctttc tttcaattgt cgcataattt tccaaacagc agactggtat aagttctttt
    42241 ctgttttgtc taatagacct gattctatta attctaataa tccacaaacc atgatcacca
    42301 tactagatgt atctttttct tctctgctag aatcatcaaa gatcaaatcc caataacaaa
    42361 tggtatcttc tggcaaccga ttcataaaat aattggctaa tattttgctt aaatcaaaat
    42421 atttttgttc tttcaaataa cgataactta atgcaaaacc gtaaatcccc catgcttggc
    42481 ctctagccca acaggaatta tcactataac cttgatgcgt acttccatat cttggttttc
    42541 catctttgac atccatataa aatgtatgat aagttgaacc atcttctctt actaaataat
    42601 ctgctgcatt ttgaatatga gcctgagcag cttttttata tttttgattt ccagtttctt
    42661 cactcgccca atacaataat ggaagattca tgcaacaatc catgatcatt ctaccttgtt
    42721 gtgaaggatc actaggatct ccccaagctt gtataactcc tgccttctcc caaaaacgct
    42781 gaagtaatgt ttctgcagca tcaattgaag cttccttcat ttttcgattt cccgttattt
    42841 tataatctgc tacggttgat aaagaataca aaaaaccaag atcgtgatga ttagtgataa
    42901 tttgacagtc aatcctgtgg ataaaatcta tatctgcttt ttcagctgtt tgttgaaata
    42961 aaggatcacc cgtcttctca aataacaacc aattaattcc tgtccaaaat ccttctgtcc
    43021 aaccaatatt gtctcttaat ggataacgtc cttttatagg acactcgctt gggaattgat
    43081 actgactgtt actgatacgt ttgattaacc tctccgtgtg tctgcgacaa cgctcgatct
    43141 cattattcca ttctttttcc attaattctt tataacattc atttatccat ttttctggat
    43201 gtattatctc ttcaaacatc actttatcaa gcatttcccc accagccttt agcataattt
    43261 tagtcttttt ttattgtttt tattttggat agttagcccg gttgtcccat caaaatcaat
    43321 tttgggacga ttgttccata cttctaaact ataatcccga tcttgatgag caaccactcc
    43381 tgttccgagc caatgggttc cttttccaaa tgtaccctct aatccaggaa taaaagtgtt
    43441 ttcccatgtc atcaagtttg tattgggcaa acccataata gctgtggatt ttcgtttaat
    43501 agtatcgtca cttaaacaga taatacctga aaagccagct tcactcaccc tatagttttc
    43561 cttgtcttct tctatgatta ttccttcttt ctcaggagaa cattcaatag caaaccctgt
    43621 ctcatataga acatattctg tatctaattc tactttgtgt acgcgaatct gccagccttc
    43681 aaaaggaata agccaagttc tgatttttgc ctgtaaagta ggttcccatg tagaggcaag
    43741 tacctctttc ttacatacaa tgtctcgtac attatttcga acgatgaact gtccataggt
    43801 tttgatgctc tcattcttaa aactggtcgt gatatatgtg tccgctcgct gaatccctag
    43861 cattgaatcc attgccattt ctgctttcaa ttgattcgct cgcggtatcg aaaaaccaaa
    43921 ttgactagaa taagcaaatt ttaaatattt ttcattggtc aatgtatgga aatttggccc
    43981 taattgtccc gcattcaaaa aaaatgtatg tccattatca tgttgtatga gcaaattggc
    44041 ttctgaaaga agtttagttc cttcacgaat aggcataggc atttctttag ccgtccaata
    44101 ctcatggtct tcaggtagcg ccaacaataa aaaaatctta ttcagccata atggagataa
    44161 cgtactatta tagggttcag tcaatgtcaa ttgagggtag gtataaccaa tattcaggat
    44221 acctttttca tcaaatattt ctttttgcat ccaccatctt atattccgat tgactaaccc
    44281 cttgatttca cctaatttaa aaggcaaaag atcggcccac aacatagctg accaaaagct
    44341 tgaaactgca aaacgataaa tcatgcttcg accaaaaggc acatttgcac catcttccgc
    44401 aaaaaaatga atatagtctc tagaaaaatc aatagctcgg ttcttatata tctggctaat
    44461 gggcttttct ggttctagta cgctataaat caaaccataa taatgaaatg caaaaggatt
    44521 ataataatct tggcgacctc ttgaactgtc tcgataccag ccattttgaa gataacaatt
    44581 ttctatatta tcaaatgctt ctttcagtgc tgattcttta actggataac caaatttttg
    44641 gataattgtt ccgacaatta tcttaaaaaa ttgccaatta ccatctgcac attcatattc
    44701 aaaaatttgc gagaaccaat gaataatctg gcttttttct cctgtatcta attttttcca
    44761 taataattgt tcatgatgat ataacgctaa taacattgcg ggcatttcta ccatacgctg
    44821 gtctctttca tgtatttctc cccaataatc gggatgggaa ggatctgttc cagcttttat
    44881 tcctgataga attaatttca accatcgttc atctaattgg tcaattgcgg gccccgctcc
    44941 ccataaaacc cgagagaatc cttccattcc tgcaatttta tttccatatc ctaccccttg
    45001 atgtatataa tcaatttgtg cgccacttgc tcgataaaac ggctggagtg gttgatttat
    45061 ctttttaaat aagtcaaagt aatcctgtct ggtccttaat agtggtactg gatgtttcat
    45121 tgtattttct cctcttttta ctaccaaata gctaattccg actgttctaa tcgaagtaag
    45181 ccttcaataa aaaaataatc agaatagatc agtggcacat tataagtcag ttctctatgg
    45241 tatgcttctg ctccgcctcc aatgatccca tcttcatcca tattccaatt tgcatagtgc
    45301 ttttctgtct tttggagtat attgatagca gcttcatgat acatttttga tcgtacagag
    45361 gaaacatatc ctgctaattc cagaagaccg catgcagcgc atactccagc actggtatca
    45421 atattcgaat cttttacagc aaaatcaatg agaggaatat aaccagtttt ttcgattttc
    45481 tgaataaaat ttactgctat cttttctgct atagctagat agtacggatt ttttgtgtaa
    45541 cgataactta acgcgaatcc atataaagcc catgcatttc ctctgctcca acaagaattc
    45601 tcattgtatc cttgcccgcc aagtaaccga atatatttcc ctgattctgc atcaaattct
    45661 gcgatatgcc cggttgatcc atctttcctc actagatact tagctgttgt atctgcatgc
    45721 tgccgagcaa ttttagagaa cctcggatca ccagtttccc tcgaagccca atacagtaat
    45781 ggtaaattca tcatactatc aataatcatc caaccaggtt tatggtcatt ccatgctact
    45841 aaatagttcc ctgaaatatt atagcgtcca gcaagtaccg tagctgcatg taaccctagt
    45901 tgtcgactat gtttgttttg agactgttga taatgtgcaa tagccgatgg taagaataaa
    45961 aaacctacat catgatgaac gtctgaaaaa tggtacaggg cagattctaa taattgttct
    46021 tgttgtattg ctgcctcttt gaacttttct cgttttgtct tgtgaaagag ctgccataaa
    46081 ataccactcc aaaaaccatt ggtccaccag tcgattcctc tgtcctttat tacgtctgta
    46141 tattttccgt tgtaagaaat atatggaatt ttattcccca ttctgtctaa ttccgcatct
    46201 atcttttcag aagctacatt caaaattttt cttgaccatt ccttcgtctg ttcattcatg
    46261 gatatcccgc cattctttta atttttatga atgtggagtt gctcctagaa cgaactagat
    46321 ggttaacatt cgttattttt tttacttatt tgcagctcca atgtcattct ccttaaattt
    46381 agactgcgcc cacacataaa tcaagaccat tgggatcact gaagttgcta aaattagcat
    46441 aattgcgcca taattaattg gctcacctgt actagaaacc gtcatatttt tgataattaa
    46501 gggaaccgta tatttatctt tatctgaaag catgactaat ggaaatagat attgattcca
    46561 acttcccata aagataagta aaccactagt aataatcgta ggtgataaaa tcggtacagc
    46621 gattttcgta aaaatcaccc actcattggc accatcgatt ctggcagcct ccatcagtgc
    46681 tgtaggaaat tgactgctat attgtcgcat cataaacact gaagttgtac tggcaatcga
    46741 agggatgatc acacctgcat aagtattcga taatcctaaa cgactcataa tgtaaaataa
    46801 aggaatcaat gtggtaaaaa aaggcaccat cctagatagc ataactgtat tgaacaacag
    46861 tttattcccc ataaaacgat actttgctaa tgcataaccc gccattgtag aactggctgt
    46921 agctaaaact gtactgatta cagcaataac aatcgaattc caaataatca agggataatt
    46981 atatctcgcc atgatggctt gccagttttt taatagcata tccccaaact tgaaattgat
    47041 tccgccactt aaaatttctt gattactttg cgttgaagct acaatcatat ataaatatgg
    47101 catcaaaaat attaaggcac caaacacaat aatgacgtac gtaaaaattc tgctttgtat
    47161 cttttgttta gtcattttta tcacctccga atttaaattg aatagccgat aatataacag
    47221 atacaaacac catcgtccag gcaatagcag aggcttttcc aaaatcaacc atttcaaaac
    47281 ttgttttata taaattgact cctacgacca aagctgaatt ttctggtcca ttggaagtcc
    47341 caaagaatat atttgggata ttgaaattac ccaagccatt gatagtggct aaaactgtgg
    47401 ccattaaaat gattggcttt agcatgggga tcgttaataa aaagaattgc cgaaatttcg
    47461 atgctccatc tatttcagca ctttcgtata tctctttaga aatcccttga agaccagcta
    47521 aaaataaaat agtgttatat cctacactaa gccaaatcat cgtgatgatc attgatattc
    47581 gtatccaaaa aggatttgtt aaccactgta caccgttaat tcctagcgca ttcaaaaatc
    47641 cattgaataa cccagtcgga ttgaaaaaag tcatgaatac ggaagctaca gccaccgttg
    47701 atgtaatggt tggaatataa tagacagttc taaaaaatcc ttttcctttt aactgtgctg
    47761 aattcaataa aacagcaaag taaatagcta atagcatcgt tattggaatt gatcctgcca
    47821 tgataatcaa aacattcgtg taagatttaa taaacaatgg atctcggaag atagctagat
    47881 aattgtcaaa tccaacaaat tcaaatgagt agccaatttt cataaaacta aaagcaaacg
    47941 agataatgat gggaatcaaa atcgtcacaa taaagaaaat aacaaaaggt gtgaccaatc
    48001 cataagcact ttttttatga gacgtatctg ttgctttttt cttgctgttc tttattgtct
    48061 tgcttggtaa cgaagattgc atcatcgtta agtcctccta atatttttta atcaccagtt
    48121 aattttacgc cgtacttatg agcaaaattt tctgcttctt ttagatatga attttcaaaa
    48181 tcacctgttt gccaatagtt atatgcagcg gtagtcaaat aagacgtagc atctgaataa
    48241 gtggctggta aaacagtatt tgatgtattt tcattgattt tattcaatag ttcatagtat
    48301 ttttgtcctc cttagtagtc aaactttttc tcagctgctt ctgtttgata ggccaatgag
    48361 ttagatgtag ccacgcccag ctccagtgcg ttggctaagc attcttcatc tgtcagcata
    48421 aatgtaagaa attgttgtgc cacttgtgga tttttggcat ctttagggac ataaaaactt
    48481 gatcctcctg atactgcacc ttttcctgga ttttcttttg aaaaaggaat cagtggagcc
    48541 atgcgccatt tgtcagatgc atcaggaaaa ttcaaagaca tattcgtagc ataccagccg
    48601 ccagcaacaa acattgcaga ttcttgaaat gctttctcag catcttgtgc accgtagaaa
    48661 tgtacaatat ctgagtcgat catttctttg attaactttg cagcatgttt cgcttcttta
    48721 gtattcagac ggatatttcc ttcttcatca agcaaagatt gattctgctg ggctagcatg
    48781 ttgataaaca attcttggtc cccagattct ggcaaggcaa ttccaaaact accagttttt
    48841 tcttttaatg cttttgccac ctggatatat tcttcccaag aactgatttc ttcaaaatct
    48901 acacctattt cagcaaattt ttttggttga taataacaaa gtgctggggt aaaatcacta
    48961 gcccaaggaa taatcgtttc attactcgtt tgttgacgca aaatattcaa tttttgatca
    49021 taaaactgat cgccatattt gtcataaaat ccatattcat cagcactata aaaagcatct
    49081 tgaaattttt ccagcacacc tgctgctgaa gtgtctgcca tggagataat atccggcata
    49141 tcttcgtgtg ttaccaattt tggggtgatc atttgtactg ttgctgttcc cgaagctaca
    49201 ttcacaattt ttaagtcaat atttgctcca gttttgcggt tatagatttt tgcagcattt
    49261 tcaacatagt ttgtcagttt gccgtttgcg acatatactt ctaatgtagt ggcttttttt
    49321 atcattggat ctgcgatgtt tgctgttact tcttcgttag tttttccaca tgcacttaag
    49381 ctgaataata gggtaatgtt tagcaaagct atcaccaatt tgtatctttt tttcatgtct
    49441 atcccttctt tcttttcaag ttcttatata aaacgctttc acttatgtat attaacggaa
    49501 ttagtaacac aaatatttat ataaaaaaat attttacttg tcgttttggc ttttcatttt
    49561 tgttataata gtcattaatt ataaatagaa agctttttat ataaaaagag aaaggaaagt
    49621 ggttcttttg ttaacaaaca accaatcctc caatcttgtc aaaaaaacac taacctattc
    49681 catagatacc catcttcatt tatctgctgc tccctatttt cgactactaa taaatgaaag
    49741 cgctaattta tatgctgaat ttgcacataa atctatagaa ctagataatc cttccctttt
    49801 attcattgat aaacagcaag aagtccaaat cttttctact caaaaggaaa cacaaacttt
    49861 tatcattatc cagttttatt ttgatgagca attattagca catattacgg gacaatcttt
    49921 ttctatagat caagaaataa catctatcat tacttttctg aaaaatgaac agttcaaaat
    49981 tgattatcta aaacatagta agccagctat ttcctatgat caggaaatca attctttttt
    50041 catactatgc caacagatga gcattcattt gctttcctta ttagttactt attgtctgca
    50101 aaaaattcaa ttgtcttcct taccaaggca ttatcaacag gaacttgctt tattgacaat
    50161 accttcaaat gagaaagtca ttcctaaaaa aaagcatatt ctttattcag atgaattagt
    50221 tgatccaatc atacgatatt tagaagaaca tctttcagaa aattttttgc ttgaacaatt
    50281 agcccaaatt ttttttatca gtccagccag cttaaaaaaa cattttagaa aagtgaatgg
    50341 aaaaagtgtt attagctatt ttaaagaact aaaaatggaa aaagcaaaac gtctcgtgct
    50401 agaaaatgaa ttaacctata cagaaattgc agatatatta ggatttcatt cgatccatca
    50461 tttttcttca gcttttaaaa aatatacagg gttatcccct tcaaagtatt atctatcctt
    50521 aaaatagaaa aaaattttta ggttttatcc acaaattatt tctctatatt ttctgtctat
    50581 tcaaaataaa caataatttt ccggcacaat tatttgctgt tttttaccat aacatagtac
    50641 gatcaacgta gaaaaggaga tgtttttatg aaagttgttg tcattggcgg tgtagctggc
    50701 ggtccttcat ttgccactcg tttccgtcga ttgaatgaag cgcacgaaat cattatctat
    50761 gaacgcggag aaaatatttc ttatgcaagt tgtgctttgc cttattattt aggaggtgtg
    50821 atcactgacc gtgactcgct gatcgaacgt acaccagaaa tattgaaaac aaaaaacaac
    50881 atcgatgtct ttactaaaca cgaagtaaca gcaatcgatc cttctactaa gcgattaaca
    50941 gttaaagacc tatccacaaa tgaagaaata aaaacagatt acgataagtt gatcatctct
    51001 tctggtgcta gaccagatta tccggatatt cccggagttt ttgaagcaga aaacggcttt
    51061 gtcctccgta gtgtgacgga tgcggatcga atcaaatcgt tccttgaaga aaaaaatcca
    51121 caacatgtcg tcattcttgg tgcaggtgtt atgggtctgg aattagctga gaatttcaag
    51181 catcgcggct tagacgtgac tttaatcgat caattgccac aagtcgcttt cccttatgat
    51241 ccagaaattg ctaatttagt ttatgacaaa ttgctgaaag aaggattagc cgttcattta
    51301 gaaacaagag ttactgagat ccgtgataaa ggtcgagaaa tagtattatc agatggctcc
    51361 gtcctttctg ctgatatgct aatttttgct gttggtgttt ctccgaagaa tgaagtggtg
    51421 aaagcaaccg gcatacaatt atcagataca ggtcagatca ttgttgatga ccagttacaa
    51481 accaatctgc cggacatcta tgcgattggc gatattatcg aaacgactag tgtagtgact
    51541 ggtcagccga tccaaagtat gctttccagt gcggccaatc gtcaaggaca tatgctggca
    51601 gatattttaa atggtacgcc tatgcgctat cgcggatata ttggtgcagg tgtcgcaaaa
    51661 atctttgatc atacagcaag ttatgcggga atgacagaac atgaattaaa agcatcaggc
    51721 ataacaaatt ataaaactgt ttttatcact ccctttgacc atgcctattt ctatccagaa
    51781 gctacaagat taaatctaaa gctgattttt gatgcagata gcggtcgtat tttaggtgga
    51841 caagcatttg gagaaaaagg tgtcgataaa cggatgggag aactttctgt agcaatcacc
    51901 ggaaacttga cagtctttga tttgcctgat ttggagttgc cttactctcc accgtattct
    51961 actacccgtg acccgttgaa tatagctggt tatgtcgcaa tcaatcaaat gacgaatatc
    52021 gtagaaacga tcaaagcaag tgatataccc gaaaacgatt tgaaagaagc gttcttttta
    52081 gatatacgtg aacctaataa agcaccttct ggcagtatct cggcaacaaa aaatattcca
    52141 atgaatgagt tacgagatcg aatcaatgaa atcccaaaag ataaaaaagt ttacattact
    52201 ttcagaagag gattgaatac ttatacttct gcccgaatct tggcaggttt gggtatcaaa
    52261 gcgattttga ttgaagaata aaaaaacgac cgcggtgggt agccgcggtc taggagttat
    52321 ttttacttga ggagtaaaaa tgaaaaagta tatagggttg ttggttggta tgtatataga
    52381 atagaagata aatgtgaaga aattgtgcat tatttcttca catttattag gttatagttt
    52441 aagaaagttt aaacattgcg caatttaatt attttagctg tttcttttca taaaaaaaca
    52501 tgttgtgact ctgagacaaa tcatagtcca tagtgatgtc tctagagtaa ctacgcctaa
    52561 tattacgagg catcatatta atctatcagc actagtacct aaactagcat ccctttcgaa
    52621 tattttttct tatataataa agtttgcttt cactcctcta atctcaccaa atcgactggc
    52681 ttgcgttgtg tgatactagg aactgattgg tttagaacga cttttctatt gtaatcatat
    52741 ttttcgccat cctcgttgtc ttctaatcct tcaatgaatg atccctaatg tgtgtattaa
    52801 ataatttaat ttttttgttc ctatttattt a
//
LOCUS       DACDYJ010000013        51270 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDYJ010000013 DACDYJ010000000
VERSION     DACDYJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.013"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            330..1175
                     /locus_tag="IWM51_000753"
     CDS             330..1175
                     /locus_tag="IWM51_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9970190.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="IWM51_000754"
     CDS             1456..2676
                     /locus_tag="IWM51_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP9970191.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="IWM51_000755"
     CDS             2673..5393
                     /locus_tag="IWM51_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9970192.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="IWM51_000756"
     CDS             5377..6321
                     /locus_tag="IWM51_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9970193.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="IWM51_000757"
     CDS             complement(6390..7400)
                     /locus_tag="IWM51_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9970194.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="IWM51_000758"
     CDS             complement(7580..7924)
                     /locus_tag="IWM51_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9970195.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="IWM51_000759"
     CDS             complement(7917..8351)
                     /locus_tag="IWM51_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP9970196.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="IWM51_000760"
     CDS             8489..9226
                     /locus_tag="IWM51_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9970197.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="IWM51_000761"
     CDS             9219..10430
                     /locus_tag="IWM51_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9970198.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10637..11416
                     /locus_tag="IWM51_000762"
     CDS             10637..11416
                     /locus_tag="IWM51_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP9970199.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="IWM51_000763"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="IWM51_000763"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP9970200.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IWM51_000764"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IWM51_000764"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP9970201.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="cpaF"
                     /locus_tag="IWM51_000765"
     CDS             14132..15061
                     /gene="cpaF"
                     /locus_tag="IWM51_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP9970202.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="IWM51_000766"
     CDS             15018..16067
                     /locus_tag="IWM51_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP9970203.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="IWM51_000767"
     CDS             16064..16342
                     /locus_tag="IWM51_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP9970204.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="IWM51_000768"
     CDS             16317..16787
                     /locus_tag="IWM51_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP9970205.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="IWM51_000769"
     CDS             16804..17106
                     /locus_tag="IWM51_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970206.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="IWM51_000770"
     CDS             17123..17494
                     /locus_tag="IWM51_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP9970207.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="IWM51_000771"
     CDS             17466..17825
                     /locus_tag="IWM51_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970208.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="IWM51_000772"
     CDS             17997..19004
                     /locus_tag="IWM51_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9970209.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="IWM51_000773"
     CDS             19029..20213
                     /locus_tag="IWM51_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP9970210.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="IWM51_000774"
     CDS             complement(20650..22233)
                     /locus_tag="IWM51_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9970211.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="IWM51_000775"
     CDS             complement(22811..22954)
                     /locus_tag="IWM51_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP9970212.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23177..23656)
                     /locus_tag="IWM51_000776"
     CDS             complement(23177..23656)
                     /locus_tag="IWM51_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9970213.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23729..24013)
                     /locus_tag="IWM51_000777"
     CDS             complement(23729..24013)
                     /locus_tag="IWM51_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970214.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24091..24285)
                     /gene="ssrS"
                     /locus_tag="IWM51_000778"
     ncRNA           complement(24091..24285)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWM51_000778"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24357..25643)
                     /locus_tag="IWM51_000779"
     CDS             complement(24357..25643)
                     /locus_tag="IWM51_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP9970215.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25781..26452
                     /locus_tag="IWM51_000780"
     CDS             25781..26452
                     /locus_tag="IWM51_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP9970216.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26418..26906
                     /locus_tag="IWM51_000781"
     CDS             26418..26906
                     /locus_tag="IWM51_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP9970217.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26919..27866
                     /gene="prmA"
                     /locus_tag="IWM51_000782"
     CDS             26919..27866
                     /gene="prmA"
                     /locus_tag="IWM51_000782"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP9970218.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27867..28631
                     /locus_tag="IWM51_000783"
     CDS             27867..28631
                     /locus_tag="IWM51_000783"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP9970219.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28864..31077
                     /locus_tag="IWM51_000784"
     CDS             28864..31077
                     /locus_tag="IWM51_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP9970220.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31088..31534
                     /locus_tag="IWM51_000785"
     CDS             31088..31534
                     /locus_tag="IWM51_000785"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP9970221.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31705..32964
                     /locus_tag="IWM51_000786"
     CDS             31705..32964
                     /locus_tag="IWM51_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP9970222.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33053..34000)
                     /locus_tag="IWM51_000787"
     CDS             complement(33053..34000)
                     /locus_tag="IWM51_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9970223.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34394..35686
                     /locus_tag="IWM51_000788"
     CDS             34394..35686
                     /locus_tag="IWM51_000788"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP9970224.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35701..37470
                     /gene="aspS"
                     /locus_tag="IWM51_000789"
     CDS             35701..37470
                     /gene="aspS"
                     /locus_tag="IWM51_000789"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP9970225.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37579..38454
                     /locus_tag="IWM51_000790"
     CDS             37579..38454
                     /locus_tag="IWM51_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9970226.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38720..40603
                     /locus_tag="IWM51_000791"
     CDS             38720..40603
                     /locus_tag="IWM51_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9970227.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40678..40962)
                     /locus_tag="IWM51_000792"
     CDS             complement(40678..40962)
                     /locus_tag="IWM51_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9970228.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40952..41686)
                     /locus_tag="IWM51_000793"
     CDS             complement(40952..41686)
                     /locus_tag="IWM51_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP9970229.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41815..42438
                     /locus_tag="IWM51_000794"
     CDS             41815..42438
                     /locus_tag="IWM51_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP9970230.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42630..43751
                     /locus_tag="IWM51_000795"
     CDS             42630..43751
                     /locus_tag="IWM51_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP9970231.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43758..46886
                     /locus_tag="IWM51_000796"
     CDS             43758..46886
                     /locus_tag="IWM51_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP9970232.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46905..50099
                     /locus_tag="IWM51_000797"
     CDS             46905..50099
                     /locus_tag="IWM51_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9970233.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50245..50952)
                     /locus_tag="IWM51_000798"
     CDS             complement(50245..50952)
                     /locus_tag="IWM51_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9970234.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16782 a         8955 c        10641 g        14892 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaagg attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatcccaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggttt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg agactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccatggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttta
    23041 ttttacttga actcattgac tgttttccct ttattttaaa cattataaag accgatgagt
    23101 gcttgccgcg tcttttgttt ttatcacggt tttttgtgca aaaaaacctg ccgatagttg
    23161 gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc tggcaattgg ttttctaaat
    23221 cagttcggac aacaagcaca tcgcaagctg cgtttctaat aacatattca gaaacagaac
    23281 caataaataa acgttctaca gcattcaatc ctgtggctcc taacataatc aaatctactt
    23341 gattatcttc tgggatttct ctggcaataa tttgttttgg tgagccatat tctacgacag
    23401 aggttacatt attcaagcca gctttcttcg cattgctttc ataatctgcc aatgtttgct
    23461 ttgccatttc agtagcttgt tctgcaagca ttccatcaaa ggatgaaacg gtttggaaag
    23521 aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt atttcggata gctacattga
    23581 ccgccttttt aaaagctaat tcagcttcat cagaaccatc tacggcaacc atgatttttt
    23641 tgtattgttg taacataagc attcactcct caagtcaatc gtattgtact cttttattgt
    23701 agaacacttt agggaaaaag agaattattt actgctttgg ttcagcaatt ttttttgcaa
    23761 atcggatact gaaggtggca gaaacaataa ttacgatcag taaaaagacc aaggcaataa
    23821 agcgttgatt gaaaaaagct gttaaaatac ccattacgcc taaaacagca tatgcagctc
    23881 cgtaaaattg taggaatcct tgatttttat catttttgtt gatcaataca aaaaagatat
    23941 cctgtttatt aaataaataa tagctaacga ataataatac taaagtggcg ataatcatta
    24001 aaatcctaat catgaacaaa tcctccaaaa taagcaacaa atagaaaaag caatttgcca
    24061 agagcaaatt gctttattct ttaaaagatg gtaatctccg ttaatgccgt tgtttgtcct
    24121 gtagtattga tcccgcgaat tcaagcaggt gggtcccatg gtcgtagctt cgaactacca
    24181 aatgataagg catgttacca gcagtgacac aagacgggtc ccaagatatc aataaaaatg
    24241 ttcggtcaac acacaaagag gacaattcga acatcacaga tttcccacct ctatagtagc
    24301 atatgaattt aaaaagagca atgagagagc tcgcagaaac agaaccgttt tcttgtctag
    24361 ttttttggag gatgcttttt ccactctttt atacggtaat actgttgacc cagagcctgt
    24421 tcatatttcc ctgtatcttt tggctgataa tactgagcat tcttcagttt atctggtaaa
    24481 tattgctgat tgacccaagc ctgatcaaaa tgatgaggat actgataacc cacgccacgg
    24541 ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat gatcaggaac atctccagct
    24601 ttgccctctc gaatatcagc aagtgctgca tctaatgcca tgtaggcaga attggatttt
    24661 ggcgacaagc aaagatcgac gacgacgtct gctagtggga tacgtgcctc aggcaatccc
    24721 aatttttcag cagctaatac agcgttcact gtacgagccg cagcagcagg attacctaaa
    24781 cctatatctt cataaccgat aaccatcaga cgccggcaaa tacttgccag atcacctgcc
    24841 tcaactaaac gggcgagata atgaagtgca gcatccacat cacttccacg aatcgatttt
    24901 tgaaaagccg aaataacgtc ataatgagca tctccatttt tgtcatgggt aagagctttt
    24961 ctttggacac attcttcaat gatcgacagt gtaagatgaa tccttccttc tttatctgaa
    25021 ggagtcgata gtgtagccag ttccagccca ttcaatgcac tccgcaaatc gccgttcgtt
    25081 gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga ttgcttgttg tccaaggccg
    25141 cgttctttat cccttaaagc atgttcgact gccaactgga tgtcttgttc tgttaatggt
    25201 ttgacttcaa agatctgtgt acggctgcgg atcgctggat tgatggtaat ataaggattc
    25261 tcagtagtcg ctccgatcaa gatgattcgg ccactttcta aatgaggaag cagaaaatcc
    25321 tgttttgttt tatctaatcg gtgtacttca tccaataaga ggatcactgt accactcatt
    25381 tttgcttctt ccgctacaac ttgtagatcc ttttttgaat ctgtagcggc gtttagcatg
    25441 cgaaatgcat aattagtaga gccggcgatc gcacttgcga tactcgtttt tccagtacca
    25501 ggaggtccat atagaatcat ggaagagagc atgcgagcat cgaccatgcg gcgtatgatt
    25561 tttccttccc cgactagatg ctgctgacca acgacttcat cgatcgttcg aggtctcatg
    25621 cgataagcca atggctgttg cataggattt tcctccttct tgtttttacg tcccttaatt
    25681 atatcatatt tagcaagatg cctagaaaga acagccttct gttgttttgt tttcatagct
    25741 tatccaacta atattgtgta tgataaagta ggtgagaaaa atgacggata aacaaaagat
    25801 agatgaattt ttttggaata aaacgacgcc agaagtggca cgggatttat tggggatgta
    25861 tttggagcat gacacaccag aaggaagatt agctggatat attgtagacg cggaagcata
    25921 cctaggtcca gaagacgagg ccgcccatag ctatggttta agaagaacac cgcgtgtgcg
    25981 ggcaatgtat gaaaaaccag gtacgatcta cctttacacg atgcatactc atcggatttt
    26041 gaacatcatt actcagccag aagggattcc tcaaggagta atgatacgtg caatcgaacc
    26101 cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga aaaactggtc cagatatcag
    26161 caacggtccg ggaaaattag tggaagcatt agcaattcct caagaacttt atggtcagtc
    26221 tatagcagat agttcgttac gcttagtttt tgagaaaaaa aagacaccaa aaaagatcaa
    26281 tgctttgcct agaatcggta tcccaaacaa aggtgtatgg acagaaaagc cgttgcgttt
    26341 tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa aaccaaatcg agaaagattg
    26401 gggttggagg aaagaaaatg aaaaagaagg atctattaac atatttagat gaaataattg
    26461 aaaaacaagt gacagactat gatgtagctt tagattggga tacaaaaaat catacgatcg
    26521 agatcgttgt tcgcttgttt gcagaaaata aagcacaatt tgagattgat gatgcagaag
    26581 ggatcgtttc gcaagaacca attatcgaat ttgaagacgg cgtacttctt tttaatcctc
    26641 agaaatctgt ttttgatgaa cgcaattatc tagcagtaat cccttatgaa ggaaaaaaag
    26701 ggttggcaaa atcagtagct gattctttag tggaatatct aaatgaagta ttagctcaag
    26761 ggcaaagcga tttgcttgat tttttggatg aagacgatga cgaagctgta ttcgagcttc
    26821 actgggataa tcagcaacta gcagaattag tagaagtaaa acagcaaaat gaagcagata
    26881 cttatttacc gtacccaagt tattaaggag tgacagatat gaaatggaat gaagtaaaag
    26941 tagaaactga aagtgaagca gtcgaagcag ttgctaatat tttgatggag gcaggggcga
    27001 gtggtgtcgc gattgaagat tctttagatg ttgaacattt tcagtcggat caatatggtg
    27061 agattttaga taaaaatgat tttacatcgc tgaaagaagg cgcactagtc atggcttatt
    27121 ttccggaaac gactttctta ccggaaatct tgccttttat caaagatcgt atcacacaat
    27181 tacctgagtt tggtctagct attggaaaaa ataaagtaac agttagtgaa gtagaggaaa
    27241 gcaactgggc aacagcctgg aagaaatact atcatccggt gcgtattacg cgaatgctga
    27301 cggttgcgcc aagctgggaa aactaccaga cagaagatcc tctagaaaaa attatcacac
    27361 tagatcctgg tatggcattt ggcacaggca ctcatcccac gacttccttg acattgacag
    27421 cactagaaac agtcttgcgt ggtggtgaaa cattgctaga tgtgggaact gggtcgggcg
    27481 tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt ctatgccttt gatttagacg
    27541 aagtagccgt tcgttcggcg aaagaaaata tggatatgaa tgaaatagcc aaagatgtcc
    27601 atgtatcagc taacgatctt ttgacaggaa tcgagattga aagtgatgta atcgttgcga
    27661 acattttagc agatatcatc ttattgatga tcccagatgc ttggcgttta ctgaagcaaa
    27721 caggcacgct gatcgtttca ggaatcattg aagagaagaa gcaaatggtt ctggatgcca
    27781 tgttagaaca agggtttatc gtggatcaaa ttttccagca aaaagactgg tatgcaatta
    27841 ttttgaaaaa accagaggtg gaatagatgc agcggtattt tttaaaagaa tcatatgaat
    27901 caaaagacta ttttgtttta gaaggggaaa actatcacca cattgtacgg gttatgagga
    27961 tggaacccga ggatgaagtt tttttggcat tcaatgatca aatagccatt gttgcagaaa
    28021 tcacggagat tactgaagag gctgtttatc tgaaagaaat taaaaaagaa gaaaatcaaa
    28081 aggaactgcc aagttcgatt accatagcga gtggctatcc taaaggggaa aagctggatt
    28141 ggatcgttca gaaatccact gaattaggag cgcaagcttt tcttggtttc ccggcatcag
    28201 cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg acaacaacgg cttgaaaaga
    28261 ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt gccatcagta gagttattgg
    28321 aaacgaaaca agatttattt gagcggctag atgattatga cgtggtcttg gttgcttatg
    28381 aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa aattcttagt gaaacaaaac
    28441 cagatagtcg aatcttagct gtctttggac cagaaggtgg attatcccgc gatgaagtcg
    28501 attattttgt agaacacaaa gggatacttt gtggattagg accaagaatc cttcgtacag
    28561 aaacagcacc tctttattta ttaagtgctg ctagctatca atgggaatta atgaatcctt
    28621 ctttcagcta acgttcttca ttaataaaag agtatcgaca gaaagaattg aaaaaatagt
    28681 caacaatttg tataatagaa acaattattt atacaatgga tacgaaaaat cgtttgaaaa
    28741 agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa ctgcacggat tttgggacaa
    28801 ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa aaaagaaagg gggatcgatt
    28861 cctgtgacaa aagaagaaat tttaacggga cccggtgtca taaagctcgt tagtcaatac
    28921 atgggtcccg aacatgtagc atttgttgag aaagcatgcg aatacgcgac tgctgcacat
    28981 gacggacagt ttagaaaatc aggcgaaccc tacattattc atcctatcca agtcgcaggt
    29041 atattagcag atttaaaaat ggatccccat acagtggcta caggcttctt acatgatgtt
    29101 gttgaagata cagaaatcac tttagaagat ctgagagaag aatttggcga tgacgttgct
    29161 atgttagtgg acggcgtaac caaattaggg aaaataaaat ataaatccca cgaagagcag
    29221 ctagcagaaa accaccgaaa gatgctgcta gcaatggctc aagatttacg agttatcatg
    29281 gtcaaattag ctgatagact acataacatg cgtacgttga agcacctgcg agaagataag
    29341 caaagaagga tcgctcagga aactttagaa atctatgcgc ctcttgcaca tcgtctaggg
    29401 atcagccgga tcaaatggga attagaagat acagcacttc gttatctaaa tccaaaacag
    29461 tattaccgta tcgtccactt gatgcagacg aagagagaag aacgtgaaaa atacgtaagt
    29521 ggtactgttg aagatattcg aatagcgaca gaagagctgg ggatttttgc agaaatctat
    29581 ggacggccaa aacacattta ttcaatctat cgtaaaatga aagatcagaa aaagcagttc
    29641 aacgaaattt atgacttgct agctattcga gtgatcgtag attcaatcaa ggattgttat
    29701 gctgtattag gagcaatcca cacaaaatgg aaaccaatgc ccggcagatt taaggattat
    29761 attgccatgc caaaagctaa tatgtaccaa tctttgcata ctaccgtgat cggtccggca
    29821 ggaaatccgg tagaaattca aatccgaaca caggaaatgc atgaaatcgc tgaattcggg
    29881 gttgctgcac actgggccta taaagaagga aaaaatgaaa aagtagaacc agatggtatg
    29941 acgaaacaat taagctggtt ccatgagata ctcgaacttc aagacgaaag ctatgatgct
    30001 tctgaattta tggaaggcgt aaaaggagat atctttagtg ataaagtcta cgtcttcaca
    30061 ccaaaaggag acgttactga gttaccaaaa ggatccggac cattggactt tgcatacagt
    30121 atccatacag atatcggtaa caaaaccact ggtgcaaaag taaatggcaa aatggtgcag
    30181 cttgattaca aattgaaaaa cggagatatc attgagatca tgacttctcc aaattcattt
    30241 ggcccaagtc gcgactggtt gaaattagtt gctactagca aagcaagaaa taagatcaaa
    30301 cgtttcttca aagcccaaga tcgagaagaa aatgtgatca aaggccacga atccgtggtc
    30361 aaatgtatta cagatctagg atttacgcct aaagatattt tgacgaagaa caaactgcaa
    30421 gaagcactcg atcgttttaa ttatcaaaca gaagatgatc tctatgcagc tgtagggtat
    30481 ggagaagtta gccccttgac gatggccaat cgtctgactg aaaaagaacg taaagaacaa
    30541 aaaatcgagc agcaaaagca agaagcagaa gaaatcatga atcagccgaa aaaagaacct
    30601 gacaaaatga aagtacgtca tgaaggtggc gttgtcattc aaggggtaga aaacttacta
    30661 attcgtatca gtcgctgctg taatccgatt cctggtgatg atatcgttgg ttatatcact
    30721 aaaggcagag ggatatccat tcatcgtcga gattgtccga atgttcagcc tgacaaacca
    30781 aatgtagcag aacgtttgat tgaagtcgaa tgggaagata catcgaatac acgaaaagag
    30841 tatgatgcag atttggaaat ttacggctat aatcgttcag gcttattgaa tgatgtactt
    30901 caaacagtca atgcgctaac gaaaaatctc aacagcgttg aagcacggac gaataaagat
    30961 aaaatggcga cgatccattt gacggttggt atccagaatt tatcccacct aaagagtatc
    31021 gtggataaaa tcaaagcagt acctgatgtc tacagtgtac gccggacgaa tggataggag
    31081 gacagatatg agagcagttg ttcaacgggt aagtaaagct gctgtgtcag tcgatcagca
    31141 agaaattgga aaaattggct acggcctact tgttttatta ggcattcatg aaaccgatac
    31201 gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa atgcgtattt ttgaagatga
    31261 acaaggaaaa atgaatatta gtgtagaaga tgtgggagga caaattttaa gtatctctca
    31321 attcacactg tttgcggata cgaaaaaagg aaatcgacca agctttgtgg aagccgcacg
    31381 accagaaaca gctattcctt tatacgaatc attcaatgaa ggaatcagaa gtcgcggcat
    31441 cacagtcgct accggacaat tcggagcaga tatgtcggtt tctctcgtca atgatggacc
    31501 tgtaacgatc attattgatt ctaaaaacaa ataaagtaat ctggttttca ctagtaaaaa
    31561 ctatgaaaca cgtcgcttaa gttagactga taaactctaa cttaagtggc ttttttgtct
    31621 gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt aagcttaaat tatacaagtt
    31681 attttttata ggaggttgct gaagatgaaa gaagtaccag tagtagatat caaaatcaca
    31741 gatttaaaaa aattgtatca aaaaggatac aacatgttgg aggagttacg ccatgaagcg
    31801 gacgctcccg tcgtcaaagc aaagattttc aataaagaag ctatcacgat ctacggatca
    31861 tcagcagcaa aagtatttta tgatccaagg aatttcaaac gaaaaggtgc tatgccaaaa
    31921 ttagtattga agacactttt tggtcaaggt ggagtacaga ctttggatgg agctgctcat
    31981 caccatcgga aaaatatttt tatggatcta atgacaccgg aaagaatgga agattatcat
    32041 cgtatcttag ataagaatct gacacaagca cttgaagcac agcatggtca atttgaacta
    32101 tttgatttaa gtaagatggt tttctttaca tctatttgcg agtgggcagg tatcaattta
    32161 tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat atcaaatctc tatgatcagc
    32221 ggaacgttta cttctccgat tgatcacata aaaggtgtcg aaaatcgcaa aaaatcagaa
    32281 aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc ctgtggctgg aaaagaaaat
    32341 gtggcattat atgcttttgc caatgccaca gatttagatg gtcagctttt acctctggaa
    32401 gtggcagctg ttgaattatt gaacatcatc cgtccaacgg ttgctctaac tgtgtgggcg
    32461 gcattgatgg gacatgcact tttcagtaga ccagatttgt atcagcaatt aaaaaatgac
    32521 ttttcgacgt tacaagatcc gtttattcaa gaaatgagac gctattatcc tttcttccca
    32581 atgctacctg cgatttcgct gaaagaagta gaagtagatg gttatcgtat accagaaggc
    32641 agttgggtta ttttagattt atatggaaca gaccatgatg aacgaacagt tgaagcgcca
    32701 gattccttta tgatcaaacg gtatgtaggg aaagcaaaag atatttccta taaagaagaa
    32761 tatgagatga ttgcacaagg cgggggaaat tttcgacaaa tgcatcgctg tgcaggagaa
    32821 tggatcactc ttcatagtct acgtgtgttt tccgatcagc tagtaaataa gtttgagttc
    32881 tctgtacctg aacaagattg gacgattcca ttcaatcagt ttccgactta tccgaatagc
    32941 cgagcgctac tttacaagaa ttagattagc ataaacttat cgaaaaaaag gaagatcaaa
    33001 cagtgctgat cttccttttt tcatattact ataaattgat aagatcaatt gatcaaaatc
    33061 cacgaaatgc ttcatcaatg gtttgataat cttcattgga taaatgcaaa tctaaagctt
    33121 ggacattgtc agatacttgc tctggacgct tagctccagg tataactaca gacactcgtg
    33181 gattcttcat gtaccaagct aaaacaagtt gcgcaggagt ggcttggtat cttttagcaa
    33241 tggggcgaag tacatcgaca gcggttaaaa tttctctgaa acgttctcct tggaaatctg
    33301 gatttctttt tcgaggatct ccttcaccaa attgtttttc ctcaccaagt tcatattttc
    33361 ctgtcagcaa accggaagca agaggaaaat agggaacaaa actgattttg tttttttcaa
    33421 gataagggaa taattctttt tcagcctgcc gatggatcaa actgtattta tcttctacta
    33481 catcgacata tccatctgca ttcgcttctt tcaattgttc taacgtgaag tttgacacac
    33541 cgactgctcg gatttttcct gcttctttta attcgtgcaa tgtagcaact gattcatttt
    33601 ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc gatgtaatca gtttgcagac
    33661 gttttagtgc atcttccacc gattgtttga gaaactcagg agaattatca aatgttcgcc
    33721 ctttatttgg tacatgagca gcttttgtag cgatgacgac acgtgagcga tcatattctt
    33781 tttccttcag tacctcgcca atcagttctt cggaacggcc gtttccgtaa gcataagcgg
    33841 tatcgatcaa attgatcccg ctattcaaag ctgtacgaac tacttgtttt cctgtctcat
    33901 cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat ccaagctgtt
    33961 ctgcgtagac ttgtgaatgt ccgattctta tttctgccat agctgattcc tcctttttgt
    34021 ctagtctaaa acaaaatacc aaaaagctga actaaaacgt ttttgtgagg ggaaatagtc
    34081 agatatctct tgactttgag actggttttt agtaggttat aagtaatatc gaatgtcaat
    34141 gaaagagcga gtaagttttt gaaactttac aagagaggat catcgaggct gaaaatgatc
    34201 taaagtttca ttagcctaaa gacactctgg agatggacga gtgaaaagcc gtcccgcgta
    34261 cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat taggtggtac cgtgtatgaa
    34321 tttcattcac ccttgttcat gatgaacaag ggtgattttt ttatttacat agctaagaaa
    34381 gggagagaaa actatgagtt tccaaagacc aaaaggaaca aatgatattt tgccaggcga
    34441 atcagaaaaa tggcagtttg tcgaagaaac agcacgttta ttattcagag attatcagta
    34501 taatgaaatg cgtacaccga tttttgaaca ctatgaagtg atatcccgaa gtgtagggga
    34561 tacaacggat atcgtttcaa aagagatgta tgatttttat gacaaagggg aacgccatgt
    34621 gacgcttcga ccagaaggta cagcaccaat cgttcgttct tttgttgagc ataaattgtt
    34681 cggcccagag tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt ttcgctacga
    34741 acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag catttggatc
    34801 agaaaaccct gcaactgatg tggagagcat ggtgatggca cttgatttct tcaaacagtt
    34861 ggggatcacg caaatccggc tagtcatcaa ttcattaggt gataaagaaa cgcgtcaagc
    34921 ctatcgtcaa gcattgatcg actatctttt accttttgaa tcagagctga gtgaagactc
    34981 taaacgccgc cttcatgaaa atccattacg cgtattggac agtaaagaca aacgagatca
    35041 aaaatttgtg gcagaagctc catctatctt agattatcta agcgagcctg ctcaaaaaca
    35101 ttttgatacc gttgtttcca tgctggatgc attggctgtt ccttatgaaa tcgacagtaa
    35161 tatggttcgc ggactagatt attacacaca tacgattttt gaaatcatga gtgacgcacc
    35221 aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc tagtggaaga
    35281 actaggtggt ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc gattactctt
    35341 gactatggag gcagaggaag tagttatccc tgcatttaat gagttggatg cttacgtggt
    35401 cgcactcgga gacgaaacaa atatcgaagc attgaaagtg gtccaagcga ttcgaaactt
    35461 tggtttttca gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat ttaagacagc
    35521 agataaagcg aatgccaaat tagtattggt gatcggtgaa gatgagcttg ctagtcaaac
    35581 gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt tttccattag aagagatata
    35641 tgaacatttt gatgaagtct atgatgaaat gactttattc gagtaggagg aaaaaaataa
    35701 atggcacaaa gaacgatcta ttgcggatta gtttccgaac aacaattaga acaaacagtt
    35761 acattaaaag gatgggtaca aaaaagacga gaccttgggg gagtaatctt tatcgaccta
    35821 cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa agaagcatgg
    35881 gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag tgactgggga agttagaaga
    35941 cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg aattcgaagt gattgcaagt
    36001 gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc aaattgaaga tgatcaagca
    36061 atcagtgacg aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc aaaaatgact
    36121 aaaaacatca aattaagaaa tgcgacaaca aaagcgattc gtcattattt agatgataat
    36181 gactttctcg atatcgaaac accttaccta gggaaatcta caccagaagg cgcaagagac
    36241 tacctagttc cttcacgtgt ccatgctgga catttctatg cgttgccgca atctcctcaa
    36301 ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt attatcaaat cgttcgttgt
    36361 ttccgcgatg aagatttacg tggagaccgt cagcctgaat ttacgcaagt agatattgaa
    36421 acaacgttct taacagcaga agaaatccaa acttacacag agggattgat tgcgaaggta
    36481 atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac ctatgatgaa
    36541 gcaatggctc gctacggcag cgacaaaccg gatacacgtt ttgatatgga attgatcgat
    36601 ctaagcgata cagtcaaaga agtagaattc aaagtcttcc aaatggctct tgaaaacggc
    36661 ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc gctactcacg aaaagacatg
    36721 gatcagttag gtcaatatgt aggacaattc ggagctaagg ggcttgcatg gctgaaagta
    36781 gaagaagacg gattgaaagg tccaattgct aaattcatgg gagaagcaac agaagcaatc
    36841 attaaagcaa cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga taaaagtgaa
    36901 atcgtcgctg ctgcattagg tgctatccgg acacgtttag gaaaagaatt aggactaatt
    36961 gatgaatcga aattcaattt cttatgggta actgattggc cacaatttga attttcagaa
    37021 gaagaaggtc gatatgtctc tgctcaccat ccattcacga tgccaaaaga agaagatatc
    37081 ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat cgtcttgaac
    37141 ggttatgaac taggcggagg ttctatccgt attcatactc gtgagcttca agaaaaagta
    37201 ctagaaactc taggctttac aaaagaacaa atggaagaac aatttggttt cttgttaaca
    37261 gctttagatt acggattccc tccacatggc ggtattgcat taggattgga ccgttttgca
    37321 atgttattag ccggagaaga taatattcgt gaagtcattg cctttcctaa aaacggaaaa
    37381 gcagcagatg tgatgagtga tgcaccaagt atggtttcgc ctttacaatt gttcgaattg
    37441 aatattgatg taacgaatat agaagaatag ccgataaaaa ggtaaaaagg atttgtcgaa
    37501 tacgcttctt ttgacaacct tgatttaatt gaaaaacact ttataatgaa gccataacat
    37561 ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca tgattacacg
    37621 accaagcttt cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt gaatttcttt
    37681 tatcagccag gacatattta ttcaagtggt attactggtc tagctcagat tttgacgact
    37741 ttgtcaaaat cactgatagg attcgaagta ccggtatcaa caactttgta tttactgaat
    37801 atacctttgt ttgtattggc gtggttaaaa atcgggaaga agtttacaat tttcactttt
    37861 atcacagtca ctttgacctc cctttgtatc catattttac cggaaacagt cctatctacc
    37921 gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat tgggctagcg
    37981 ttgagaaacg gcctatcatc gggcggtcta gatatcgtga gtatcacgat tcgtaaaaaa
    38041 acaggaaagt cgattggttc tatttcgatc tacttcaatg cattgatcgt ttttgttgcc
    38101 ggaattctat ttggctggca atatatgttt tacagtacgt tgtctatttt cgtcagtgga
    38161 aaagtaactg atgtcgttta tactaaacaa aagaagatgc aggtaatgat cgtaacgaaa
    38221 catccagaaa aagtagtaaa tgaaatccaa aactgtatgc gtagagggat cacgatcatc
    38281 aatgaagcag aaggtgctta taagcatgat aagcagacag tattgatgac gatcgttaca
    38341 cgatacgaac tgcctctact tagagaagca atgaaagctt ctgatccaca agcttttgta
    38401 agtattgcag aaaacgtcca gatacttggt cgtttctatg aggatggcat ttagatcgag
    38461 tttgacaaaa attcatgaaa tactgctgta gacagacgtt cgtttgtctg cagttttttt
    38521 ctaccatagt agaatcagca aagaaaaact gcttttttat ttgaaatttt cttaaagttg
    38581 aaaaaaagca tgtttatttt tttaaattta tgggatgata gaaaaaaata gtagagaaag
    38641 gacacgattc tacttgctat tttcacttaa tacccgtata ttacgaatga aaaaagttta
    38701 tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc gctacatcac
    38761 tgggtttgat gggctccgtt cattagctgt ggtgggtgtt atcttctatc accttctgcc
    38821 aactagtatg aaaggcggct atttgggcgt tccgattttt ttcgtcgtat ctggttattt
    38881 gatcacggat ctgctgcggc aggaatggga acaaaatgga aaaatcaata tttggcaatt
    38941 ttatgtcaga agaatgaaga gactctatcc ggcacttgtt tttatgcttg tgacagcttc
    39001 ggcttatatc actttgtttc agcgagggct gttaaacaat cttcgaggag ttgtggtcag
    39061 cagcttactg tatgtcaaca actggtggca aatcaataac ggtttatctt atttcgatcg
    39121 atttgctaat gagtcaccgt ttacgcatat ctggtcttta gcagtagaag gtcaaaacta
    39181 tttggtttgg ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa gaaaatggat
    39241 cttttatact gtgttaggta catccttcct ctccgccatc ttgatgatga tcttgtatac
    39301 accaggaggc gatccaaccc gtgtttatta cggtaccgac actcgtctgt tttctatatg
    39361 gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa acatcccgaa
    39421 acaagcgaaa aatattctga atatcgctgg aggaatctcg cttttggcac tgattctttg
    39481 cttcttcttt ttagatgatc attacaattt catctattac ggaggaatgc tcctagtgag
    39541 tctgttatgc gtagtgctag ttgcagtgac agcacacccc ggagcaagtt tgaatcgatg
    39601 gctgacaaat ccagtattta cttggattgg caaacgaagc tatggcattt atctgtatca
    39661 gtttccagtt atgatttttt atgaagcaaa ggtgactgat attgccgatc atgttttgtt
    39721 gcatacttta gtggaaatca cattgattct aatcatcagt gaactttctt atcgatatgt
    39781 ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc caagtgatga aaggatggtt
    39841 cagcaaaccg atcatcagta agagcaaacc atgggttgtt ccaggcacat tacttgtagc
    39901 aattgcactc gttggtttcg ttacggcacc gaaaaacgct gtaacagccg atcaaaaaca
    39961 actgcaggca aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa acaattcaga
    40021 aaacaccact agttcggttg accaagcgat ccttgataag tacgagttgt ccaaggcaca
    40081 agggaataag gcacaaaaat tagaactaac ggcatttggt gattcagtta tgttggatgc
    40141 tgcaacggat ctacaagaac tttatccaaa agtagtggta gatggagatg tcggacgaca
    40201 attatatgcc agtgtaccgt acattgaaaa attaaaggaa caagatttgt tgaaagacac
    40261 tgttctgatc ggactaggaa cgaatggctc attctcggaa gctcagtttg atagtgtgat
    40321 ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga cgaagcgctg
    40381 gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca atatgacgct
    40441 gattgattgg tacagctaca gtaatgacca tagtgactgg ttttatgatg atcaagtcca
    40501 tccaaatcct gaagggatga cacattacat tcacttagtc agcgaagcct tgctaggagg
    40561 aagttcaagt acgaacagca gtgagacaaa cgtgacagaa tgatagctac aaaaaacttc
    40621 aggcaaaaac atttgttttt gcctgaagtt tttttatatt taccgtgttt ttaacattca
    40681 tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt tttgttttcg
    40741 ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag aaaatacttc
    40801 tgcatggatc atcttaacag gtcttctttt ttgaagacga gtatatttag caccagtacc
    40861 attgttatgt tcctctaatc gtctagaaag atcggttgta tatccaccat aaaatgtctg
    40921 atctttacag aataatacgt aaaaataatg ttcatttgcc atagagcatc tcacgaatct
    40981 ctgcagtgta ttctccatcc atattgtaag tgatcaatgg tggagcgact ttgaatccgt
    41041 ctaatttgcc ttctttgatt ccttcaacta acaaaatatt tgcctctttt ccaggttttg
    41101 ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc tgaatgattt
    41161 ctaagaaccg atcaggacga tggaccatag taaagcgtcc atttgttttt agtagcttac
    41221 ttgaaatatc gatcacttct tctaacgtcg tatgaatctc atgacgggcg attgccaggt
    41281 gtttattagg gttcgtttta tttgttggca gccctttgaa ataaggtgga ttacagacga
    41341 ctaagtcaca agaattatgt cgaacgacag ataagctgtc tttcagatcg atggtgtgga
    41401 cagtcatttg ttcatttaaa tggttaagcg cgatactacg tcttgccatg tctgctagac
    41461 gaggttgaag ttcgatagcg tcgatagccg cctgggttcg tttacttaaa aaaagtccaa
    41521 ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca cgggaaaagt
    41581 gagcaagcag cacagcatct aaagaaaaag aaaacacttt ggaactttgg ataatcttga
    41641 tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg aagcatggat tccctccaaa
    41701 atgaaaaaaa tagtcgtctg tattatacta tttttattaa aaagaaccaa ggtggcttgt
    41761 caaacctcta ttttttatgc atactatgac cataagaagg gaaaggaaga gactatgttt
    41821 tatcgattta tgcgcggggt agttcgagta ctattagcct tagttaatgg aaatgcccgc
    41881 tatgagaata aagaagtttt accgcaagat gagaattaca tactagttgc tccccatcgt
    41941 acttggtggg acccattata tctggcggta gctgcagcac ccaaggaatt cagctttatg
    42001 gcaaaagaag aactgttcaa aaatccagtt ctgcgattta tattaactcg atccaatgct
    42061 tttccagtta aaagagaaaa tccaggacca agcgtcatta aaacacctgt aaagattttg
    42121 aaatcgacaa atttgagttt gatcatgttt cccagtggga caagacatgc tacagaactt
    42181 aaaggcggaa tggcattgat ttccagaatg tctaaagcga aaatcgtgcc agctgtatat
    42241 caaggtccgt tgacacttgg ggatctgttc aagcgtcaga gagtgacagt gcgtttcggc
    42301 gaaccaatcg acttatcaga tatcaagaaa acggataaag aaggtatggc ggagatcgaa
    42361 cgacgaacac aagcagcttt tgacaaattg gacaaagaag tgaaccctga tttcaaatat
    42421 gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc ggtcttttga atctactgtc
    42481 ctgttcagtt cagacttttt caatgaaaac gagggagaga cgtcaagtct gccctctttt
    42541 tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag tgttataata
    42601 attagaagga cattttttga ggtgattgaa tgcgtttttt acatacagcc gattggcata
    42661 tcggaaaaaa actacaaggt tatgacttga ttgaagacca gcgtcatgta ctatcttcta
    42721 tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga gacttatatg
    42781 ataggagtgt cccagctgtt gatgccgtag aattatacaa tgaattgatg gtcgattgga
    42841 atttaaagga aaagatcccg atttttgcta tatcaggaaa tcacgatagc agtacgagac
    42901 tttcagcggg tacgccatgg ttcagttatt cgaactattt tttgcgaaca cagctagcag
    42961 aagcatttga accagtcgag tttaaaaacg tacaattttt ctttcttcct tactttgaac
    43021 caattgcagc gagattatat tttgaagatg acaatatccg aacgatcaag caagcggtag
    43081 ccaaggtgat agataaaatg caggagctat ttgattcaga aaaaaaacaa gtattgatta
    43141 gccatttttt tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg
    43201 ggttggattc gatccctggt gaactactta caccgtttga ttatgtagca ctaggacatt
    43261 tgcataatca agctgctttg agacaggata atgctcgcta tagcggttcc ccattgaaat
    43321 tctctttatc tgagatcaac caaaaaaaag gcgtatggat cgttgacctg acaacaggcc
    43381 tggagttgaa ttttagggag atcgaacccc tgcatgatat tgaagaaata acagcgagtt
    43441 tcaaagaatt gctggatcct gccttttatc agtccgtcaa tcgggagaat tacatctata
    43501 tctatctcac tgatagaacg atcatcccga atatgatgaa ccagcttcgg caaatatatc
    43561 ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa
    43621 aagtactaga aatgaaagat cctcaaaaat tagcagaagc attttttagt gaaatgacgg
    43681 aagagcagct aactgcaaac caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg
    43741 aaatggaatg atcaagaatg aaaccgaaaa aattacaact taaaaatttt ggtcctttta
    43801 tcaatgagac cgtagatttt tcacgtttga cagaagcacc gctttttttg atcagcggga
    43861 agacaggagc tggcaaaacg acgatttttg atgggatcac cttcgcactt tttggtgaaa
    43921 cttccggaag acttcgctct ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag
    43981 aaacaagcgt gactttttct tttgaacatc agcagatgag ctatgaaatc gaacgaaaac
    44041 cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa
    44101 cgttgaccat ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa cgtacagaag
    44161 cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa acagttttct caaatcgttt
    44221 tactacccca aggagaattc cgtaattttt tggtttcatc aagcagtgat aaagagactg
    44281 ttttacgtca tttattcggt actcaatttt tccaacagtt caatgagcga ttaaaagaaa
    44341 aagcaaaaca acagcaacaa aaattggatc atttagagca agagctgcat cttctgcaaa
    44401 aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc gttttagccc
    44461 aatggacaaa ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa gaagctctaa
    44521 cggaacttca ggaaaaacaa aagcaactag aaaattccta ctatcgtttt gaaagcttga
    44581 aaaaaagtta tgaagaaaaa acgatattaa taaacaagca aaaggaatta gctgctcaaa
    44641 aagacgaaat agaggaaaag aaacgatgga ttgggtatta tgaatttacg caacgattag
    44701 tcgaaccaat ccagcgccta caagaaataa aagttgaaaa aatagaactg tcacaaaaaa
    44761 gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag tggcaaaaaa
    44821 atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa aaactgcaaa
    44881 acaaacagat gatggtgcca attgtcgaat ccttacgtag taaaaaacaa gacgcagaac
    44941 aattaacaat ttcattggat aaagaagaat tgaatgccga gaagctgtat caaaatctcg
    45001 ctatctatca ggaaagaact cgaattcttc aagaaaattt actggatcag gaaaaactgc
    45061 aagaagatcg tcttgctttt ggacagttaa aatatctaga tgaaaagcag caagaaatag
    45121 aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat aagagaagaa ctcaaaaaag
    45181 caactgaaga aataacaaaa aatagtcagc tattgaaaga acagacagaa caagcagaac
    45241 agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt ttgcgtccag
    45301 gggagccatg tcttgtttgt ggatcaacgg aacaccccat acaacaatta aaacacgaac
    45361 agccaaataa ggaagatatc ttaaaagtag aacaacaact ggcagaagct gagtctgctg
    45421 tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat cttcaacaac
    45481 aattatcaga tttagaacaa aaaatcatgg caacgaatat acaattggct gaacaagaag
    45541 aacacttgca acaggctttg aaaaaggcat ttcctgagat aacagaacaa aaaacaaaaa
    45601 caactcttgt agtaatcgaa caatctttag atagacgaaa aagcaagatg gccaatgcaa
    45661 gaatagagct ggaagaaata agacaacaag cagaaaagct cgaacaacaa ttagctgtct
    45721 acaaacagca gcagacagaa aaaaataatc agctccagca actgcaagga gaaatcaaaa
    45781 gtttagaaca acaattgcaa gatacggata gtaactgttt agaagaggag atacgacgac
    45841 ttgaaaaaca gttagaagaa gactcgaatc aactagctgt tgagcagaaa gcaggagtgc
    45901 aattgttgca agatcttcat acgcttcgga cacagcttga tcttcaacat gaacaaatag
    45961 aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag ttagaaagcc
    46021 agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa gcacaatata
    46081 aagaaaacca acgtacagtt acttcttatc atgaagagca attagtgaca gctgaccggc
    46141 tgcgtcaatt agaaaatatc cagctaactg agacattccc agatacagaa gagatcagcc
    46201 aacagttaga agaaataaga cgtatgatcg aaataaagca agaaaagata tacggtttac
    46261 aagaacaaaa aattagtaat caaaaaatct acgatgaatg tctttcgatt tatcacacaa
    46321 gccagacgaa attagatgag cttacgcaat tacagcagct ttcgcaaaca ttcaacggag
    46381 aaaatcctaa gaagactagt ttggaacggt atgtgcttca ggtttatctt caagaagtgt
    46441 tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa tttgagctag
    46501 cggataccat tggcagttat cgagggaaaa ccggattaga aatcaatatc tatgatgatc
    46561 aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt attgcagcac
    46621 tttctttagc attagctctt gccgatgtga tccagacaca agctggtggt gtagcaatcg
    46681 atgcattgtt tattgatgaa ggatttgggt cattagatga agaagctttg gaaatggcaa
    46741 tggaagcttt agaaacgatc gaaagtgaag gaagaatgat cggcatcatt agtcatgtaa
    46801 gagaattgaa agaacgagta ttacaacaaa taagaattga tacagaaggt agcggacaaa
    46861 gcaaaatacg ctatcaactt ggataaaaag ggagattggt aactatgaaa tggagtattc
    46921 cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga aggccgagtg ttatcggttg
    46981 tccctgacca agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa ttgtatatgg
    47041 ttgatttgga tggtacagca aaagagatag attactgcca atgtccctat tgggaggaac
    47101 ataaatattg taagcataca gttgctgtgg aactttattt acggtcgaaa aatctatctc
    47161 gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa agcaacaagt gagggagaaa
    47221 tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt
    47281 tgattattga ataccaagta gaaacgattg agactaatca gtatcaccca gagctttcta
    47341 ttttggcact ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa
    47401 aaaacatcta tgatttttta caagcttaca cagaaaaaga aacttacact gttaataaac
    47461 agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc
    47521 atcttgcagg aagtgctcag acgcatcaat tattaggtac gaacggctta caagtcaaag
    47581 gaaaactaga taaaagatat ctgcttctcc cagtagaaca agcccgcttc ctgttagagc
    47641 agatgaatca gactacacgt ttgcatctgc agataaatga acaaaacgat caaggaatac
    47701 aattttccga tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga gaagattatt
    47761 tgctgcaagc attgaatgat tttgattttt tctttatgca ttatcagtgg ggagttatcc
    47821 aagggacatt ttattctctg acgaaatatc aacaaacggt ttatcaaact tggaaacaat
    47881 tgattcgccg attggagaaa ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt
    47941 ttaaagagat tatccctctg ctgtcagaga tcggcgacgt agctattgcc cctgcagtag
    48001 aagaggaagt agcagatatc cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa
    48061 aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat ctattcgact gacccgaagc
    48121 atgaggtctc gacagaacag gcttttcagg tgttacgtga cacagtagaa gagaaacgag
    48181 tacttgatct attcagaatg tatcgctacc agaaaaatga aactggttat gaacgtccat
    48241 tgccaagcgg ggaagaacta tatgcctttt tccgcacaga actatctgtc ttccgtcagt
    48301 ttggtgaagt cagacttgga agaaaattgc gagagttgta tttagatgcg cataaacatc
    48361 aaccgaaaat cgaagtaacc gagaccggtt catggctcga tattcgattc gatgtgacag
    48421 ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt
    48481 atacattaga aaatggtcag gttctttctt ttgattcaga agaatttcaa caaacaagcc
    48541 aagtgttgca acaattacgg gaatcgatac gtacagaaga aggaacgatc catgtaccta
    48601 aaaatcaagg attgatcatt caaaatcaat tggaaaaaag caatgctact tttagtgaat
    48661 cgttccaaac gatggtacaa gatctcattc atccagaaag ataccaggca cagcttccca
    48721 aaggattgaa tgcaacgatg agagattatc aaaagcaagg ttttcgatgg ttgaagatgt
    48781 taggtcatta tcagtttggc ggtattttag ccgatgaaat gggacttggg aaaacactac
    48841 agacaattgc ctttttactt tcggaaaaag aagaacggaa aagcttctct gctttgatcg
    48901 tagcgcctgc gagcttaatt tacaactggc aggcagaggt gagaaaattc gccccttctt
    48961 taagtatcca agtaatcaat gggaataaaa aagaacgtga agaattactg gctaaagaca
    49021 cagatatccg agtgacttct tatgccagct taagacagga tctagctaat tatcagtcgc
    49081 aaaaaattga ttatttgatt cttgatgagg cacaaatggt caaaaatagc agtacgaaaa
    49141 ctgcacaagc attgagagaa ttagctgtac ctcagcggtt tgcactgagt gggacaccaa
    49201 tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac gattatgcca ggatttttcc
    49261 cgactaaaac aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt
    49321 ttattttacg gcgagataaa caaactgtgt tgaaagactt gccggaaaaa acagagatga
    49381 atctatattc tgcacttaca gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa
    49441 tgcgtgaaga aatcacatca atggattctg aagcattcaa gaaaaaccgg atcgggatac
    49501 ttgctggtct gacaagactg cgacaaattt gctgcgatcc tcgtctgttt attgaagatt
    49561 accaaggcgg ttccggcaag ctcgaacaag taaaagatct actggtagct gccaaagaga
    49621 acaaacgaag agttttgctg ttttctcaat ttacagggat gctgactatc ttgcaagaag
    49681 agttagccga gctagggatc tcgacatttt atttaagagg cagtacgaaa ccacaagatc
    49741 ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg atttccttaa
    49801 aggctggggg aactggattg aatctcacgg gagcagatac ggtcatcttg tatgaccttt
    49861 ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa
    49921 atgtggtaga agtgtggcgt atgatctctg aaggaacgat tgaggaacgc atggattcac
    49981 tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa gaacagttga
    50041 cgaaactgac tgaagaagac atccgcatga ttttaagtgt aggagaaatc gaggagtaag
    50101 agactcgaaa aagcgttcag taatgggtaa gaaagccttt ctaaagaagc gattgtttat
    50161 ttgatttaaa aaatagcaag gaggctggga ttccatttta tcccagcttc cttgctatca
    50221 tttttttatt tctattttta aaggttatac aaagattgtc ttcattgtct ctaacagctg
    50281 atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt tcaattttgc gttctcgatg
    50341 gttgaaccaa agcgcttgcc agttagcact ttttgcacct agaacatcat tgtcgtaatt
    50401 atcgccaacg tataaggtct cttctggaag catatgaaat tctttctcag ctagctgaaa
    50461 aatctctttt tctggttttt ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg
    50521 aatccaattg ttcaattgaa gttgcttcag tttttttgtc tgatggtcag tcggcccatt
    50581 tgtgataatt cctaatggga cattcttttc cttcaagaaa tcgagggttt ttttgacctc
    50641 ctcatgcaag cagatattat caagttcttc ctcgtaaatt ttttgaaata agaggccatc
    50701 ttcttccgta atatgagggt agtctaaatc ttttagtgat tggctgatcc gatgtgctcg
    50761 catatattct agtgtccaat ctcctgccat tacttttggg aagttttcat cactatgatg
    50821 gcggaaccgg atatatagtg gatgcatatc tgcatctgaa acaagcggaa cgactctttt
    50881 gaccgcgttt cgaaatggct gctgctgatc atacatcgta tcatcaacat caaacacaac
    50941 tgatttaatc aaaattttac acgcctttcg gttttcattg attttttcac aaatccttga
    51001 ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt cttactatat
    51061 ttctatttcc ttgacagaat aaggttttta tatttcagtt tttttcacta aaaatcgtga
    51121 aacacgtcaa taagtttgat gaacaaatta atcattatgg tagaagtcga ttgatttatt
    51181 ttaatgcaat ctctgtggaa ttttcgaaag atttattttt ttaatacagt ctcaatataa
    51241 ctattcaaac ataaaataac gttatttgat
//
LOCUS       DACDYJ010000014        51170 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDYJ010000014 DACDYJ010000000
VERSION     DACDYJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.014"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..641
                     /locus_tag="IWM51_000799"
     CDS             <1..641
                     /locus_tag="IWM51_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP9970235.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(1507..2037)
                     /locus_tag="IWM51_000800"
     CDS             complement(1507..2037)
                     /locus_tag="IWM51_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAP9970236.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(2034..2651)
                     /locus_tag="IWM51_000801"
     CDS             complement(2034..2651)
                     /locus_tag="IWM51_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9970237.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            complement(3233..3454)
                     /locus_tag="IWM51_000802"
     CDS             complement(3233..3454)
                     /locus_tag="IWM51_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAP9970238.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            complement(3632..5164)
                     /locus_tag="IWM51_000803"
     CDS             complement(3632..5164)
                     /locus_tag="IWM51_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9970239.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            complement(5300..6385)
                     /locus_tag="IWM51_000804"
     CDS             complement(5300..6385)
                     /locus_tag="IWM51_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9970240.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            complement(6400..7119)
                     /locus_tag="IWM51_000805"
     CDS             complement(6400..7119)
                     /locus_tag="IWM51_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP9970241.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            complement(7116..8426)
                     /locus_tag="IWM51_000806"
     CDS             complement(7116..8426)
                     /locus_tag="IWM51_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP9970242.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            complement(8453..9043)
                     /locus_tag="IWM51_000807"
     CDS             complement(8453..9043)
                     /locus_tag="IWM51_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HAP9970243.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(9361..10422)
                     /locus_tag="IWM51_000808"
     CDS             complement(9361..10422)
                     /locus_tag="IWM51_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9970244.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(10990..12057)
                     /locus_tag="IWM51_000809"
     CDS             complement(10990..12057)
                     /locus_tag="IWM51_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9970245.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(12077..12556)
                     /locus_tag="IWM51_000810"
     CDS             complement(12077..12556)
                     /locus_tag="IWM51_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HAP9970246.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(12573..13034)
                     /locus_tag="IWM51_000811"
     CDS             complement(12573..13034)
                     /locus_tag="IWM51_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9970247.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(13048..14298)
                     /locus_tag="IWM51_000812"
     CDS             complement(13048..14298)
                     /locus_tag="IWM51_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP9970248.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(14676..16127)
                     /locus_tag="IWM51_000813"
     CDS             complement(14676..16127)
                     /locus_tag="IWM51_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9970249.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(16448..17212)
                     /locus_tag="IWM51_000814"
     CDS             complement(16448..17212)
                     /locus_tag="IWM51_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP9970250.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(17240..17938)
                     /locus_tag="IWM51_000815"
     CDS             complement(17240..17938)
                     /locus_tag="IWM51_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP9970251.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(17950..18729)
                     /locus_tag="IWM51_000816"
     CDS             complement(17950..18729)
                     /locus_tag="IWM51_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP9970252.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(18745..19689)
                     /locus_tag="IWM51_000817"
     CDS             complement(18745..19689)
                     /locus_tag="IWM51_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9970253.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(19735..20514)
                     /locus_tag="IWM51_000818"
     CDS             complement(19735..20514)
                     /locus_tag="IWM51_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970254.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(20894..22969)
                     /locus_tag="IWM51_000819"
     CDS             complement(20894..22969)
                     /locus_tag="IWM51_000819"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP9970255.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(22971..23888)
                     /gene="glyQ"
                     /locus_tag="IWM51_000820"
     CDS             complement(22971..23888)
                     /gene="glyQ"
                     /locus_tag="IWM51_000820"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP9970256.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(24274..25092)
                     /gene="recO"
                     /locus_tag="IWM51_000821"
     CDS             complement(24274..25092)
                     /gene="recO"
                     /locus_tag="IWM51_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP9970257.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(25235..26134)
                     /locus_tag="IWM51_000822"
     CDS             complement(25235..26134)
                     /locus_tag="IWM51_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP9970258.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(26149..26550)
                     /locus_tag="IWM51_000823"
     CDS             complement(26149..26550)
                     /locus_tag="IWM51_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP9970259.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFSVIVGLILFVPKIYHLLFN"
     gene            complement(26528..27004)
                     /gene="ybeY"
                     /locus_tag="IWM51_000824"
     CDS             complement(26528..27004)
                     /gene="ybeY"
                     /locus_tag="IWM51_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP9970260.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(27021..29213)
                     /locus_tag="IWM51_000825"
     CDS             complement(27021..29213)
                     /locus_tag="IWM51_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP9970261.1"
                     /translation="MLNKTLQKLQKKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(29237..30208)
                     /locus_tag="IWM51_000826"
     CDS             complement(29237..30208)
                     /locus_tag="IWM51_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP9970262.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            30914..32293
                     /gene="brnQ"
                     /locus_tag="IWM51_000827"
     CDS             30914..32293
                     /gene="brnQ"
                     /locus_tag="IWM51_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9970263.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(32449..32895)
                     /locus_tag="IWM51_000828"
     CDS             complement(32449..32895)
                     /locus_tag="IWM51_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP9970264.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(32922..33098)
                     /locus_tag="IWM51_000829"
     CDS             complement(32922..33098)
                     /locus_tag="IWM51_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP9970265.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(33260..33688)
                     /locus_tag="IWM51_000830"
     CDS             complement(33260..33688)
                     /locus_tag="IWM51_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9970266.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            33780..34640
                     /locus_tag="IWM51_000831"
     CDS             33780..34640
                     /locus_tag="IWM51_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP9970267.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(34734..35027)
                     /locus_tag="IWM51_000832"
     CDS             complement(34734..35027)
                     /locus_tag="IWM51_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9970268.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(35100..36827)
                     /locus_tag="IWM51_000833"
     CDS             complement(35100..36827)
                     /locus_tag="IWM51_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP9970269.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLIDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(37041..37967)
                     /gene="trxB"
                     /locus_tag="IWM51_000834"
     CDS             complement(37041..37967)
                     /gene="trxB"
                     /locus_tag="IWM51_000834"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP9970270.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(38112..39545)
                     /gene="ascB"
                     /locus_tag="IWM51_000835"
     CDS             complement(38112..39545)
                     /gene="ascB"
                     /locus_tag="IWM51_000835"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9970271.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDKDHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     NEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDTVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(39545..40948)
                     /locus_tag="IWM51_000836"
     CDS             complement(39545..40948)
                     /locus_tag="IWM51_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9970272.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMATMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     DLIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            41178..42080
                     /locus_tag="IWM51_000837"
     CDS             41178..42080
                     /locus_tag="IWM51_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9970273.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(42121..44007)
                     /locus_tag="IWM51_000838"
     CDS             complement(42121..44007)
                     /locus_tag="IWM51_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9970274.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLKAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(44104..44955)
                     /locus_tag="IWM51_000839"
     CDS             complement(44104..44955)
                     /locus_tag="IWM51_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9970275.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQNNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(45290..47074)
                     /locus_tag="IWM51_000840"
     CDS             complement(45290..47074)
                     /locus_tag="IWM51_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9970276.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(47074..48825)
                     /locus_tag="IWM51_000841"
     CDS             complement(47074..48825)
                     /locus_tag="IWM51_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9970277.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(48967..49191)
                     /locus_tag="IWM51_000842"
     CDS             complement(48967..49191)
                     /locus_tag="IWM51_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP9970278.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            49442..51028
                     /locus_tag="IWM51_000843"
     CDS             49442..51028
                     /locus_tag="IWM51_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP9970279.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT        15054 a        10709 c         8140 g        17267 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc ttctcaacgc ccattaaacg aaaattctaa cgggattctg cgtcgtaatg
      481 gactgccgaa atcaatagat tttagagaag tgaatcagac atttatttcc agtgtcagca
      541 atcaacgcaa tcatattcca agaaaatcat tgaattacag aacaccaatc gaaatatttt
      601 tgagctatgt acaagaagca ttttattcta gcttaatttg acaaatcata taatttaaaa
      661 ttaacattga ttctcctatg aaaatatctt ttacctttct cctgtgaaaa ggaaatttcc
      721 ttttttatag tgttattgat ataatcagaa tattcatcaa actctgtatc tggagttaaa
      781 aatctacagt aacatctata agctttgcta gtaatcttct tgggttctct ctccattgtc
      841 aagcaacatc aaaacaccta cttactattc gattcttttc tgccaaaaac gagtcagcat
      901 cactcacaac ttatgtgttg ctgactccga ctagtccttt gttcaatatc aacttattaa
      961 acagatataa tatttttctt tatttcgtat actccaagac ttccatcttt ctttcatctt
     1021 atctctgaaa aaaacacagc aaagtgccct taataaatat acattagaat ttgcaaattt
     1081 tttcatctta agatatatcc tattcatatt ctttacaatc ctaatgggtg attacaaatc
     1141 tatttagttc aagttctaca ttataaacac ctttcttgat agtatcatct gctgcacaag
     1201 cgatgtcgat ccacttatta ggggatttac gtgctcaaaa gtggctattc cttttgggaa
     1261 gtccatccat aataatggtc catggatttt tttagggtct tgtcgttcaa atttctcaaa
     1321 aactttatta tacttccata gttttataat tatttggttt cactttggct cgttcaaaca
     1381 ccaacttgta tttcttttca acgttcgaac tggagccccc cctataatct cattattatt
     1441 ttcatgactt tttactaccg tggaattaca agcaacaaca atattatctg ctaaatgaac
     1501 acctttctat atcacgcttt ttgctccaat ccaacaatgt ttacctataa ctattttatt
     1561 atttttattc aaaatcctat gagtattcaa ttcaaaaatc gtatgtgaat cggtatccat
     1621 aaataatgta tcccacgata ctgtacagcc atctttgaat tgaatttcct taaagcaaat
     1681 tatactcatt tttcctgtcg aattaaactt tgacccaata tctaaaactg catttttcct
     1741 tatgtcaata tgagcccctt gtgcgataaa cgctggccct tgtattctta gtattccttc
     1801 aatacttata atcgtttctt catttcccgc agtatttaat actggaaagc ccaaataaac
     1861 tatttttcca gattgtaata ctatctcaat tttccctctc gatttgcgtg taatccgtac
     1921 tttacgatgc accaaaatag gtaatctcca cgcatctgaa atatgaagaa atttataatt
     1981 gaagtatatc gttttaggta aatttaatat attctttatt atcaatccga gtctcatctt
     2041 tcaccatctc tttttgtaat caaaattagt ttataaagga ttacacccaa tataccgccc
     2101 aacgtattat atacaaaatc cgaaatttga aacgttccta gcctcaaaaa aagttgtaaa
     2161 aattcaatta ttgctgaaaa ataaaatgca atttttcctc catatactat tgaggaccat
     2221 attttgggtt tctttatatt ttcatattta atagacatta ccatataaac atatggaata
     2281 aaaagaataa tattttctaa acacgctgta gataactctt ttgttccagt tgttgtatta
     2341 tacttataaa tccaccagtc tcccataaca tcagataaag gatttaacca aattgttcga
     2401 ttgattaatg ttgtaaataa aatcatcgca gtatagaata tcaaagcaca aacttttcta
     2461 tattttctat ccttctttaa atgaagataa agttccatta gtatacgttt atagccctca
     2521 cctgcagcaa tgccatctgt tgcatacata aaatagacca gtcctaaaat tgtcaataaa
     2581 acagcaaacc caaagggttc atataattta tataatatat ctgtaataat ttctgcaatc
     2641 actattatca taattatttc tccaatatca agtatacact tatcgcttcc aattacacat
     2701 gatcatatta gagtgtatta tcacattctc taatattcaa tttatttttc ctaaaatatt
     2761 cgaaataatc gtcttgataa cgagtaagta tttttatacg attgataaca tagtaacaat
     2821 atttttaatt atagtaaaaa atcatctgcc tttttcatcc acctaatctt atttttcatt
     2881 ttattcaaca tccttaaata aacttccgtt cctaagctct ataatatctt cttaaaaaat
     2941 aatattatgg tgtcgttttc aaactcgtaa tcttttacat tggaaaaata catacttttc
     3001 tcaatgtagc aactcctatc tcacagtatt caaaaattct tcaatattat gaaatacttg
     3061 tgcatgttct ttaaatacct tgtcagtctg tttataatcc cctactaaat tttgcattgc
     3121 tcccaactct tcgacaaatt gtcatctcca tctgtaaaca gccctaaaaa ctcatataat
     3181 gctttttcat caatattttt cccatattat tctcggtata gtttcacaag ttttacggat
     3241 tcttcatatt gtttatcttt ttataatatt catctaggga cataattact tttgctagat
     3301 ttcctgcaac aacacaatcg tctgatacgt ccttgtttac taaactattt gcacctataa
     3361 ctacattatt tcctatatgt acacccttta gtattctaga cttcattcct ataaaaacat
     3421 tattacctat tttgatcctc tccgatgatc ccaaagcttc tccatactac ttttaaaacc
     3481 gatcaatcat aaacatgagt caaaattgta actccttcat atcatctcca atttcaacaa
     3541 gagtcttcgt tggaacaaaa actatacatc gttcgcctat tcggcagcct aaaaattgta
     3601 gttactttat atatcattct gatgtctctt ctcatctttt taccaatacc gacgtaagaa
     3661 ctttcaaaat atacttaaac ttcttattac ggatattaaa cagaagaata actaaaatcg
     3721 atatgacaac cgatataact aaatttatca ctaaacttat cactttgaaa tttccgatat
     3781 taacatacga acaagcaaaa taacttaaat aagaagcaac aaaagctatt acacacaaac
     3841 ttatgagctt ccaaacatat ttacctgccg attcaaattt aaatatattt tggaaaacat
     3901 tatataataa ccagggtaaa tcaaccaaca atactgaaac aatggttgcc gccaaaactc
     3961 catataatcc gaaccaatta acgaggatga tatttaatac aatgtttatc attgctgtaa
     4021 ttaatggtct aaacctgtct gcgtaccata ttccagcagc atctttgtat aacccaatta
     4081 actgatcaat ttcatataag aaaaaatgaa ctatcataaa tataacaaat gaaatactca
     4141 taagattatc acttcccacc cagataatca tgaacggctg aaataatgta ataaagcatg
     4201 cacaactaat tgccagaaac caacctataa ttgttgtaat atcacaaaaa tctatataat
     4261 ttttttcttg actttctaca ataagactgt tacctatacc tgctatacat gattcataaa
     4321 ttattgcaaa tatactcatt atggcagaaa taataaagta ataattctga taaatcgcaa
     4381 gtactgaaag acctaagaaa gatgaaataa taattgaatc tgaagatcgt aatattgtac
     4441 cgcctacctt gtttgtaact aatcctttta ctttttttat aatatcgtca atagtttctt
     4501 tctctaactc accaacagga gtataatcag gatacatctt ttgagaaaca acagcacaca
     4561 ttaagttaag aactatttgt cccaaaatag aagccaataa atacaaatag taatttttta
     4621 agaaaatcag aacatatgcc tgcattacaa attggaatgt aaaagtaatt agcatagaca
     4681 cgttaattac atcatttctt tgatgtgcat atattagact ctttctatat gaaaataacc
     4741 aatacgacaa aacagtattt atcaaataca ttaaatacaa aactgtcata tttaaatttc
     4801 caggaatcga acctttaact aaaaatctca aaaaaggaat gcataacaat cctaatgtaa
     4861 acactatcca tccaataatt gaaaagtact ttttatatgc gttcagtaaa gcacaaatct
     4921 cttttctatt gtcttctgct ataggtttat acatacttac agttagtgca gcacctattc
     4981 ccaattccgc taaatttaac atttgaaata tagatgtgaa caggttgttc aatccagcat
     5041 actccattcc catctgataa atcataagtg tacgcataaa aaagggaatg ataatctgat
     5101 ataacttaaa aattgttcca gtaattatat tccttttagc attttgttta cgattaatta
     5161 tcatatcata tggctccaaa ctttctattt tttattgtta cctaatacta tataatttct
     5221 aaaatcttga aatgtaatag gtttcttcta gctctttgat taattacgct aattttcatg
     5281 tttggacaac cacaaaaaac tatacatttt caatcaaagc aacccattgt tttgtaattt
     5341 ctttcaaatt aaatttttgt acattattat atgcgttcag tgacattctc attgctaaat
     5401 cagtattctt agtaaacatt atcatgctct tagctaattg gtctatattc ttatcttcaa
     5461 ccaacatccc attaaaatta tgatgtacca aatctctcgg accagtttta caatcataag
     5521 aaataactgg taatccataa gactgtgctt ctaatataac cataggaaat ccttcataaa
     5581 cagaactaag cacataacaa gatgcagatt tataatattc ctccacatgg tctgaatgcc
     5641 ctgctatagt tactctttgc agatttaaat cttttatctg ctggcgaaga tctttttcta
     5701 attcaccttt tcctacaatg ataagatccc aatcatctac ttttttttct acaattttcc
     5761 aagcacttat catgtcagca aaatttttct cttccactaa tcgccctact gcaagaaatc
     5821 tctttttatc ataatgatat tcgtactcga tattagatat acacggattt ggcatagtta
     5881 taacaaaatc ttctgataaa ctatattcat caatatgttt ttgacgatct tcttctgtga
     5941 gaaccacaag cctctttgca tattttttta tcaatttttt agccttgaat ctcttctttt
     6001 ctttcagcat ttccattgtc cagtaattaa aatgttccca agaaatcaat ttgcactttg
     6061 ttcctttaat tgcccttgta gtatacaaag acaacaaagt atcaatatca attactatat
     6121 caaattcatt tttgcatata tacttgtgca atttccaaac cggatataaa taagttctat
     6181 ataattttcc ctccgtttta ggatttaaca tataatcaac cttaatcgtc ttatctaacc
     6241 caaaataagg ctctccacta ttccaaaagc ttaaaatagt gacatcataa cctaaatcag
     6301 acaagctatt ggcaataata ctccctactc tttcagttcc cccagaacga gaaattacac
     6361 cactaatgaa acaaattttc ttcaaagtac ttttctcctt tataagtgta aaaatttttt
     6421 cagtgcaagt aacctgttaa attttttttg tccaattgtt gattttaatc tataaacaaa
     6481 tttttgttta ggtgttttcc aagatgcatt atattgatga atgcttattg tattactagt
     6541 tttataaatt tttccagtat cataatcctt ggcagcaaaa taatccgtag gatagatatg
     6601 caggccattt attatttgtt cgctgttatc gcgaaccaat ccaatagatt caagataatc
     6661 tgtaatataa tacggactcg gtttcaagtt caatgtacca tcattttgat aaaaacttaa
     6721 tgaatcgtaa atatccctta aacctttaat aacagaatgt ccttcttctg caccaaaacc
     6781 aataccgcca ttaacccaga tttccccttc aaatcccata taggcatgat tttcacgaag
     6841 tatatctaac gacttaatta gctcaacatc tgtatctaaa taaaatccac catgattata
     6901 aataatatca aatcttgcat aatctggaac aaaaccccat ttacgttctt tatatgcatc
     6961 ggccatataa cggttcttat ttaaatcata atttgattca ttccattcaa taatctcata
     7021 atcagggcaa tactttttcc agctttctat gcactttcta tcttttttcg gaagtggatt
     7081 tcccccgaac caacagtaat gtatcttctt aggaatcata atatctctcc ttttgtattt
     7141 ttttcactat aataatgctc tttccttttc acaacttcag gcatggctaa aatcatataa
     7201 atcagtggcc ccgttgtttc acccctaagt gttgtgttat acacaaaaaa gaataccgac
     7261 ataattgcaa taactttaac aagacttgca tctttatcct tcataaaaga tctttttgca
     7321 ttgaaaaatg tctttattat aaaaaatagg aatatcgaaa ctcctaatat tccaacttct
     7381 ataaataacc aaatatgatg aaaatactga taatgaagat taccaaacga tttataaaac
     7441 tcactgacaa aaaaattggt aaactctgca ttgccaatgc ctactcccat taggactaat
     7501 ctaaaattat tattaaatat ttcatctact actctagaga taccagttat tcgatttaaa
     7561 tcaccaacac catcatatcc agaatctctt gacgcatatt ctaaaagacc ttgaattgtg
     7621 aaattatcag catagctttg tccattatcg ttcaatgtaa taaatattgt tataaaaaag
     7681 gataatacta tcgacacaag tattacgcat gataatatct tgatcactac tttaaaatca
     7741 acttttaaag tgaatactat tgcaacaaat attataaata attcaggaat aaaaactttc
     7801 aattcagcta atgcagacat aaaaagtgct gcacaaacac ataaaataaa atggatgaaa
     7861 tttttttctc ccttgaaata tccgattgta acaacagtaa ctgtcacaca caaaattaaa
     7921 ttcaatggca tattacaacc tgaataattc caaaaaactc cacctatcat atctcctaca
     7981 attgttccag ttggataggt gaccatgaat ctttcaatta aacaaagtgg cagtgaaatc
     8041 cagaataatt tttcaattat agcgaatact ttatagtaat ctttaattgt taaatatgtt
     8101 gcacaattta tatatactat gaacatacga gtgttatttc gtatccccca gataatcaat
     8161 cctatatttt ctaaattaat aactgagcta caaatagaaa ctagtaaaaa caaaactatc
     8221 agcatctcat ttttgctagc atatattcct tttctgcgtc taaagctaag tgcatttaat
     8281 aacacaaaga tattaattat atctcccaaa tataaaattg ctcttggggc gttaaaagta
     8341 tatacaagaa ctatggagat tgcaaaaaat gccaacgtga acaatgtcca atctgctcct
     8401 gaccatcttt ttattgaaat acgcatcgtt attcaccttc tttcgctaaa aattattcta
     8461 aaaatacttt tgttaaatta acatacccta ttatttttgc cggtacacct gcaagaattg
     8521 caccattctc tagacaatct ttgataacaa tagctcctgc cgcaacaact acattgttag
     8581 ctatttttac attaccaata atctttgcac ccacgcatac acgtacatta tttccaatta
     8641 caggacactt aggatcgatt ccatcatttc caatacagtt attcccatat aagtaacaat
     8701 tttttcctat tatggcgtta ccattaataa caattccttg tgtatggtaa aatcttacac
     8761 cttcatcaaa tacattttca ccacactcta taccacattt tcttcccaaa ctattcattc
     8821 ttctgcaaga cacaatatac ataatcgcat aaatgatatt tttctttctc tttgaataat
     8881 aataagatgt aattctcatt cgacgagtat accgccaagc gttgtagtcc ggatgtgatt
     8941 tcattatttt tagtaatttt gtgtgttgtc ctttagattc tatatataga ttcttttcca
     9001 tacgaagaac cgcttttaaa tattctttac tgctaatttc cattcatatc tcctattaat
     9061 tcaatttata cttaacaatt ttattcggat ttcttgcaac aagagcatca ttcaatgcta
     9121 ctgaatctac aacgatggat atgttatctt catcgttgat tccaccggat acaatattac
     9181 ttataaaaat cgtaacattt tttccaaaag aagggatgtc cgtgatgtat aatgcccatt
     9241 ttccgatatt tccagttatt taaacctaac gaatttttac aatagtacaa gtatcacaat
     9301 cattaatata taactggaat ataaatctcc aaaaattcac tatagtacag ttttttatga
     9361 ttaaaatctc ctatttctac ccattccgct taaatatgct aatgttttaa ccccaataat
     9421 ggattggttt agtaaaaaat agttaaatat tcgttgttta attggatttt gtttatatgg
     9481 gtatgagtga atacaggcaa tcaactcatc gttcataagt atcttagaca aataggagtt
     9541 taaatcaaat tctttattaa aactagcata tctaactgcc ttttgaatac aagtacgaca
     9601 gcgtcccaac aaaagtctcc ttgttttgtt tacaaatatc tcattttcta atatatagct
     9661 taaatcgcct tcaatttttt taactaattc aatattacaa tcgaactcat tatctctaaa
     9721 attatgtgtc aatgaattat tattattaca tctataataa tagatacaat ctttaataaa
     9781 acttgttatt tttgaatgtt gcaaatatga aatatggaat atgatatctt cagatatata
     9841 atccctttct gacggaaact tgatattgtg ctgttgaatt atctcagatg aatacagcgc
     9901 atgccaaact gacattggca atataaaatc gactttataa ctcggctcag tgccaatcat
     9961 tccaagcaac actttttcaa ttatagcgtt atttgtaaat atcgtactat catatcttgc
    10021 tggctttgaa ataatattat tatcatcata atattcataa tatccagcat aacatgtgtc
    10081 tgcattactc ctttttatac catttaacat tgcttcaata taattagctg caacaaaatc
    10141 atcagaatca ataaaagcaa catattttcc ttttacatgt tcaagtcctg tatttcttgc
    10201 caatcccaag ccagcattat ttttatgaat aatacgaatc cttgaatcct ttaatgcata
    10261 gtcttcacat attttattag atgaatcctt agatccatca tccacaatta taatatctag
    10321 gttcttatat gtttgattac acactgattc caaacaactt cctacatact tttcaacatt
    10381 atatacaggt atgattacac taattagatc actcgaattc atccttctat cctttcactc
    10441 acgttttctc aattagtcaa tgacggcatc aacgattttc ttaaattctc tgaaaaaaat
    10501 agactctgat cttgatgatg tgaatgacat actatcttct tctagaaaaa ttatttcctt
    10561 taaattattt attaaatcaa taatatgatc ctcgtaagta tgctttccct tggaataatg
    10621 aaaatatact tttgtattac acaatattgc aattttattt cttaatatga ttatttcact
    10681 cattcattac tgccacatat acacaactta taaccacccc ctgcaaagtt tagggtaagt
    10741 tatcagataa tatcttcctc ttcatcatcc agaatacaaa atctatttat ttatagaaat
    10801 atcattaatg tttttatata atgtaccgtg cttgtactct tgctctatta caagcactaa
    10861 aacactacta ttcatttgaa ttcttacacg gataaaaatt tacatcgtct atttcaatat
    10921 tttttctctc tattttattc acttaataag ttcattacat aatcgaaaca ttttttattt
    10981 tccttgtcat cactccttgc tccctttttg atacagattt aaaattctat caaagaaatt
    11041 tccatcgtta aatttcgtta ttgccaaatc cttcgaacaa cgtgaaaatt ttacgtattt
    11101 atctttatca ttgattttta gtattgcaga tgtcaacgct gatacgtcac caggttgaaa
    11161 caaatatcca ttttctccat cacgtactgc tgaagaaata tctcctacat ctgtagcaat
    11221 tacaggcatt ccataagaaa tcgcctctag aatagatata ggcaatccct cgttatagga
    11281 cggtaaaatc atcacatttg atcttctata gcactcctcc tttttttctc ccacaaccca
    11341 acctgtaaat tcaaaatacg attgcaaatt atattcttct atcaattctt tgagcgatat
    11401 ttcttcatca cctgctccag caatctcaaa aataaacttt ttttctggat attgttctgc
    11461 aatgttttta actgcttcta ccaaatcgtg tacacctttt cgctttatca aaactcccat
    11521 aaacagaaat cggcacattt ttgaattcca ctttatggta tattctggaa ctacaataga
    11581 attttctaca acaacagttt tacaatttgg ctcaatttct ttaattatct tcttccattt
    11641 ttctccaagt acaatcgtgg aatcagactc tcttaacagc aagcgaatct gattttgttt
    11701 ttttacagaa acactatcat accatttttt aaattccgat ccatgaagat ggatgattac
    11761 aggaatattt aataatctaa accacttatt cagcacatat ttcctactaa aactccctct
    11821 gtacgacatg tgcatatgaa taatattagg tttactcaat aaagagatac ctataatttt
    11881 agcaaatgca tacataaaat ataaagattt aaatattgca tttccaccct tataactcgg
    11941 tataaaagtc atctcgatat tctttttttc ccaattaaaa ttgagcattt gtgtaattat
    12001 acttgtaatt ccacctttta ccgagctgtc attaccaatc ataagtactc tttgcataac
    12061 gctttactcc ttatatttat attttttcta ttaatacctc tatcgaatta ataatcgttt
    12121 ccgtgttaga tacatacttg ttaaacatcc gaactcttgt ttctttaacg gcatcttcta
    12181 aattttctaa ttcataacaa ggatagataa tattcatctc atcaaattgt ctaattaact
    12241 gcagttggtg atcatcgaca tgctcaccat acttagccaa tcgaggaaca gcaatgaccc
    12301 ttttaccttt ttttacggcc ccaattatag cacctgtacc tccatgagta atcaccattt
    12361 ctgctttttg catagtttca gtaaactcat ccctatttag aaatgcttta taggaataat
    12421 ttagtggttt ataatcacta taaccactct gtgcaaatgt actttcgatt attactcctt
    12481 tttcaattaa atcatcaata gcttttaaca atctatcaaa ttgaaatttt tgtgaaccta
    12541 atgttataaa tatcattttt ttctcctatt atttaatata tcccacccaa ataaatagca
    12601 ttaggataaa attgtagcat agtttcccac tgaacataaa attgatctgc aaaatgatac
    12661 atcaattttc ctgtctgagt agggctggtt acttttgcaa acgattcaat ataaatcagt
    12721 ttttttccca tcatttttgt tattaagcag atagggatca tagctaaaac acctgtacaa
    12781 atcacgagat ccggtttttc ctttatatat ataataattg atttgaataa attaataagt
    12841 atagtacaca aaaaaaactt ttcttttcta tttacttgtc tcatgtagta cgttttttcg
    12901 ccttttgact cagctgaata ttctgtctgt tctgttataa caaaactctc atacttctcc
    12961 atcagtggtt ttaacatcat taactgttca taatgccccc cagaggaagc cgcaaaacat
    13021 atctttttgt tcatattaac ctctctatca gtcacgctga aaaatatctc tagtgtaaac
    13081 tttttcttta atatcatcca agcaggtatc atatcgattg gcaataatag aatcacacat
    13141 cgccttaaac tgctctaaat cgttgataac cttacttccg aaaaaagtct ctccatcttt
    13201 cagtgcaggc tcataaatca ccacagtcgc acctttagct tttacgcgct tcataactcc
    13261 ctggattgaa ctctgacgaa aattatcact gttgctcttc atggtcagcc ggtatatacc
    13321 gattaaaact ggattttccc tgttgacatc atactgatta tattcagcgt agttgtaata
    13381 gcccgccatc tgaagaacac gatctgcgat gaaatcctta cgggtacgat tagattcaac
    13441 gatagcctca atcaaatttc caggaacatc tgcatagttt gccaacaact gcttggtatc
    13501 ttttggcaag caataacctc catatccaaa ggatggattg ttgtagtgag ttccaatacg
    13561 aggatcgaga caaacaccat tgattatctg ctgagtattc agccccttca tttcagcata
    13621 agtgtctaat tcattgaaat agctgactcg cagtgctaaa taggtatttg caaacagctt
    13681 tactgcttcg gcttcagtga atcccataaa aagagtatcc acgttctcct taattgcact
    13741 tttctgcaga agtgttgcaa aaacatgagc agcatttaac aagcgctcat cagtcatatc
    13801 agtaccaaca ataatacgag atggatacag attatcatat aaagccttgc tctcgcgcag
    13861 aaactctgga ctaaaaataa tgttcttact ccccgttgtc tcacggactg attcagtgta
    13921 gccaacagga attgtagact tgatgaccat gatggtatca ggattcacgt ccatcacaac
    13981 ctcaataact gactcaacag cagaagtatc gaaatgctga gtacgactgt catagttagt
    14041 cggtgtagca ataacaacga aatctgcgga tttataagcg ctccttgcat ccaaagtagc
    14101 agttaagtca agttcttttt ctgtaagata cttttcaata tattcatcct gaattggaga
    14161 cttccgttta ttaatcagtt caatcttttc agggataatg tctaccgcgg ttacctggtg
    14221 atgctgcgcc aatagtgtgg cgatactcaa accaacataa ccggtgccgg ctactgcgat
    14281 gttgtacttt ttcttcacaa tatctcctct ttcctctctt aagtatggag aatgttactc
    14341 ctacggctat tcatcatgtc aaaataacac agcttagaat gttgataaaa taattttcca
    14401 gaatgtgata aaatctcttt catttataaa ccacacatct aattttatct attctccaca
    14461 agacatgtag agtattcttt aataacaaga tacgacctta ctaaatactt caaaaaatta
    14521 tcatatttaa ttcataaata taaatcttca ttttaaatgt atgcccaaaa atttatctgt
    14581 aatgcgtcat caaaatctag attccaaatc tttatcaatt tttatccact cattctacca
    14641 ctttagtaat attttctatt tataacactt tcgtcctaca ttgatccccc cctctgaagt
    14701 acagttttta ctgtcttaaa taaaatttta aaatctaacc ccatactcca attttttata
    14761 tattccgtgt caagttttgc tacttcttca aaatcagtaa tatcacttct accacttacc
    14821 tgccacattc cagtaattcc tggcttgata gcaattcttg cacgatgatg ataatcatat
    14881 tcttcatact ctgatttaag gcatggtcga aatcctataa ggctcatctt tcctgttaga
    14941 acgttgaaaa attgagggaa ttcatctaaa gacgtggatc taataaattg accgattcct
    15001 gtcttttttg ttccatctgg aagtatttta tttccgataa ttcttggatc aaactccatc
    15061 ttaaacataa gattattgct catcttattt tctttcatta attctacttt tctttcttct
    15121 gcatccatat acatacttct aaacttatac atcttgaacg gtttaccatt ctgacctata
    15181 cgttcttgtg caaagaaaat tgggcctggt gaattaatat aaataagtgg agcaataaag
    15241 ataaaaataa ttcccgttgc aatacatcca acaatacctg caagaatatc aattgttcgt
    15301 tttataaatg cctgttgagt tgtcataaca ttgatacttg ttgtaagtac tgtatattct
    15361 cctatacgac caatagattg ttttcccaaa gcactatctg atacttttgc aagattcaaa
    15421 tgtacagtaa ggcccatttc agagcatcgt tcaataaatt ctttcataca cgaagtactt
    15481 ttattcaaaa taataaagac ttcatctacc catccctgac ataagtaatc cgctgcattt
    15541 ttggaatcag aaacaactgt cactccctca acatcttcac caatcatgct tttatctatg
    15601 ataatgacac catttatttt atacatttta tagttatgct ctttcacatt acgaataact
    15661 acttcagcaa tactactaga tgtaactata ataagtgatt tgttcccctc atttttcatt
    15721 ttatgtttca agtaatgttt ccataagagg cgagttatat atgtaattac aacgtataaa
    15781 cctcccatca agaaaagaac gactctagaa taatcatttc caccctgaac actaatgaga
    15841 tacaaacttc ctaacagcac caacataatc gcttgcttta cagaagaagc aaattctata
    15901 tagtaccctc tttttaatac acctctatat gcttcaaaca tgaaaataac taaaagatct
    15961 gccagttcaa tgaagactgc catgttttga tacattggaa tcacataagg attccaatct
    16021 acctggcgta agacatacga tagataaaaa gcaagctgga tacacaataa atctaaaata
    16081 ataaaatcaa tatgtttaac ccatcccctt gaacttttct tgtacattcc tttttactcc
    16141 cctcttttta ataaaataaa ttgtattact tgttttctct cctaaatatc tttctgaagg
    16201 attattgact cgacaaattg gcttgtggat attcttatat atagaacatc cgtaagccaa
    16261 cttaaaactc cttaacaaaa tttttttact catattgtag atcaaaacaa gtatccaaga
    16321 aatcatattt tatttctttt ttctgcttac tcaacaacag ctaaaagaaa aatcatcata
    16381 aacaaattca actaagccaa cgatcccctt gtgaaatcaa gttttttctt gatttacgaa
    16441 taaagagcta aaataaacca aacttcttct tttttacctc ttcatagact ggataactga
    16501 tttcatcccc gttgatcaga tcctttgcga cttgtttcat ctgtactact ttttccttac
    16561 caaagtcctt tgcaatctgc tcatagcact cttttaagta aaaggtgcgc ttagagaccc
    16621 catgggcatc agaagcagcc atctgcacga gattatgttt gaccatcgtt ttggctgttt
    16681 tctggatgtc tttgccaaat accccaacat aacttggcgc agtgagttgg gccaaacatc
    16741 ccatatctag gaacggaagc aaatgatttg ggtcttcgcg aaaatgcacg ttgcgttctg
    16801 gatggacgat tactggtgtc tttcctagtt ctaacaactc aaaaaaaagc cgttctgcat
    16861 ataacggcac ttccagtgtc ggaaattcga tcagaagata tgtatcatct aaatctgtaa
    16921 ataatatttc atctcggtgg atgtcttcga tgagcgttcc ggtaatacgt acttcttgtc
    16981 cttccaataa cgtcaaaggc aactgtcttt tctctaattc ggcttgtaac gaagcgacta
    17041 gagaaatgac ttgtgatttt tcattgctgt attttccatt attgtggtgt ggtgtgcata
    17101 aaatatgcgt gatgccttgc tgaatcgctt tttcagccat tgcaatactt gcttctaagt
    17161 tttctgcccc gtcatcgatt cctggtagga tatggcaatg tagatcgatc atttctcttg
    17221 tctcctctct tcttattcct taatttccgt agtaataacc agcatctttg ctatgttctg
    17281 ctccgttgta cacaacacct aagactcgtg cttgtaccat ctccagcagt tcttttgctt
    17341 tagtcaagga ttctttacgt gacatatttt cacgtacgac caataaagtg ccatccgttt
    17401 ttaaagacat gatctgtgca tctgttacgg ctactactgg tggcatatcg aatatcacca
    17461 cgtcaaataa ctgtcttgct tcggatagga tctgatccat tcttggcgag cttaacaatt
    17521 ctgatgggtt gggtggctta ggtccactag gcaaaatcga caagttctcg actggcgttc
    17581 gttggatcac atctgctaca cttcctgatg aactaagtgc tgtacttaat ccactggcat
    17641 tgttcagctg gaatgtctta tagacgacgg gtttacgtaa atctgcatcg actagtagaa
    17701 ctcgttgacc tgatttagca aaaacgactg caatgtttgc tgcagtggtg gatttccctt
    17761 ctccaggacc tgatgaagtc acgacaatcg ttttgatttg ctgaccagct gcggaggcaa
    17821 actggatatt cgtccggatc gtacgatatt gttcggcgat tggtgaagat gggtttgtta
    17881 atgtgatcaa actgactgca tgagtttgtg ctgctttttg tttctgtgtt cgtgccattt
    17941 ttcttcctcc tagactcttg agcggcttct gcgtgatggg gctgttttat cgtttgtagc
    18001 ttttggtcgt gcaggagctg ttcctttttt acggatacca gcgttcaatt ctttcgctga
    18061 catttctgga actgttccta agattgggaa tcctaatgta tctgtcacat atttttcatc
    18121 tttcactgtc cgatcgagta attccaataa gaatgccaga cctacaccta ccatcattcc
    18181 taacattaag ccagtcatta aattcaattt attatttggc gaaacaggtg tcgtattggc
    18241 aactgcacct gaaatgatcg agattttatc aacattcatc acatcttttg cgttctcttg
    18301 gaaaacttgt gaagttgtgt tagcgatctg ttgagcagcc actgcattcg tacttattgc
    18361 ctgaatcgag aacatttgtg aattttctgc ctgattaact gagatggcat ctttgatttg
    18421 ttcagcagtc attttcacat tgtcttctgt ttctaaacga tctttgactt cattgagcac
    18481 taaatcactg acgatcatat ctttatacgt attgatcatt tgcaggttcg tattgacatc
    18541 attgacattt gcagtgtctg attgtggcag tgtcacgatc aattgtgctt gtgaactgta
    18601 ttttggtgtc aagatgaaaa atgttacgat tcctgagatg gccaagccag caaacattga
    18661 gatgatgatt attaccaagt gtttgcgtaa tgtttcaaaa atttctttca agctgattgt
    18721 ctcttccatt ttttctccac tttcctattt attctctaga ttcatttgat cttttaattc
    18781 ttttttcaca cgatcaagtt cttgatcact gactttttga taggagatat tatcttgcat
    18841 gaagccttct ccttgcagct gatcttgctt aatcgtacca aatgcctccc gataatctaa
    18901 agcaattttt ttcatttcat caaagttcat gtctgttttc atattcgact ctagagcatt
    18961 caaaacggat tgatagttgc tgacaccgtt tagactcaat acttttttcg ctacgccttc
    19021 tacgatcttt ctttggcgcc cttgtctacc gtaatcacca tttgggtctt cataacgcat
    19081 acgagaataa gataatgcct gatccccgtt caatgtaatt ttaccgattg gaaagtcata
    19141 gccatcttgt gagaaagtga ggtcattatt gacttcgata ccaccaactg catcaacgag
    19201 ttcctttagt cctttcatat tgatagagac atagtgatcg attgggatgt ctacatattt
    19261 ttgtacggta tccatcgcca tggcagctcc gccgaatgcg taagcgtggt tgatcttgtc
    19321 ttttgtgtca cgacctacga tgtcgacata cgtatctcga ggaatgctga cgatcgttgt
    19381 ctgtttgtct tcaggactga ctgtggcaac catcatcgta tctgatcttc cttgatcgac
    19441 ccgacctaaa tctccagtat caatccccat caatagaaca gagaaagatt cttgtttgtc
    19501 cagatcaact ggtttcgttc gttctccttg ggttcgttcc actgattcgt aagttttctc
    19561 aatagaatgt gagagatcca gatataattt tgctcctact cctacaattg ctaatactaa
    19621 tacgataaga actgctaaaa taatcaaaaa tatttttttc cctcttgatt ttcttcttcg
    19681 tcttgccata gtttcctccg tttgctaacc ttgcttgaat caacgtttta actgtcaata
    19741 gcttatattg ctatagccca tttgctgagc aacagtttgt gcttttgttg cgtatgcggc
    19801 tcttattttt tcattccgga tctgctctat ttgactgagc gccgtcaaat attgatcgta
    19861 agtagcggta gctgttactt gaccgtcttt cagcatcgta tcgaatagct gatctagttg
    19921 cgtgacttgt gcaatttggg tatcagcgaa tcctagatat tgcataacga ctgttttcca
    19981 agcactatcc ttaaaaaagc tcatattttc acgtacatgg gcgacatctg cacttgccaa
    20041 cttttctttg atgatgacat catccacagc ttgctgccag tttgatacgt ttttagtaaa
    20101 taatttattg acagccgtct ggatcttcag ttttgattct gcatcctcta actgcgttaa
    20161 gactgctttc ttttcttctt ggatcgcagc agcttcttta ggtaattctg attccttgat
    20221 gtcataatca tctgccgaaa gtttgataga agatagttta tgtgacaaat ttgaaactgt
    20281 ttcttctgtt atttcaggat ttaggaaaat tttttctttt gtactgtaca gcttatctac
    20341 ttcttttgat aaagaggtca cggtgtcttt ttgattggtg atggcagatt ctgcgtaatt
    20401 gatccgttga tgatattgcc gctgattatt gagatggtat ccaaacaagc aaatcacaat
    20461 agcgcctgtg cctaccgcca cagtagccaa taacttgacg aacggctttt ccatagattc
    20521 actcctaaat attgatgata gataagtaaa gatgtgattc attcacatca gaatgagcgc
    20581 tttttatttt aaaagcataa ttaaactaat atattcatag aaatatttat aaacaggttt
    20641 tccctatata atcataccac atacagtttt tttgactagg gaaattccta atttaaaagt
    20701 aaataaaaca gtaacaagat tataattaat cgttttcatt tttatttcct cacttttcat
    20761 gagcgttttg aaaattcgaa caaagaataa aataacaaaa attattaaaa tagaattaat
    20821 aacagctatt tataacaaaa aaaacagaga cacagcacgt ttcgtgcatg ctctctgttt
    20881 ttcaaacaaa agactatttt gtgatgattt gatccaagct tgctaatgac aaagccatgg
    20941 cagctatttg acttagttgt gttagtcggt tcttacgtac tgctggttca tcagccatga
    21001 ccatcgtttg atcaaaataa ttttcgatca gtggacgaag agcgacaagc tggtcgtagt
    21061 tttcgtggat cgtttggtca gcaaaacttg ctttcattgc ttctactgct ttgtgtaatt
    21121 ctttctctgc gtcgttttcg aacagctcag ggtctacttc tgattgtttt tcaagctgcg
    21181 cttttttcgc taagttcacg acacgtgtca aagcttccat tgaaggtttg aagtcttcat
    21241 ctgcaagatg ttgtttgaac aattgtgctg ctgcaaatac ttttgtcaga tctttttgtt
    21301 ccgcattcaa aacagcatcg atgacatcat gacgaatatt cttcgttgtc aataattggc
    21361 gcagacggcc tttgataaag tcgatcactt gttgttgccc ttctgataaa ccaatgccga
    21421 aacgatccgt atctttactg ataacatcgc taatcgtttc ttgcagaaca gacaaaggaa
    21481 aggcccattt tttcgattcg atgatccgaa cgacaccgta tgcttggcga cgtaaggcat
    21541 aaggatcgtt tgacccgctt ggaatcatcc caactgcaaa gaaagaaaag atgctatcta
    21601 atttatctgc aatcgctaaa acagaaccta ctgtagactg aggtagctct ccttcactgg
    21661 atataggcat gtaatgctca cgaatcgctg tcgctacagc aggtttctcg cctttcagaa
    21721 gagcatattt ttcgcccatg atcccttgta gttcaggaaa ttcccctacc atgttggtta
    21781 ctagatcaaa tttatagatt tcagcagctc gttgcaaatc catcaattct tcctcagata
    21841 gtcctacttg ttcgccaatg atacgggcaa tgacttgtac ccgttgcatt ttctcataga
    21901 tactaccgat tttttcatgg aaagtaacag tttttagttt ttcaacgtat tggtcgattg
    21961 aagctttctt gtcttcatta taaaagaatt ccgcatcttc taaacgagcc gtcaatactt
    22021 tttgattgcc tcgaaccaca tcttccaatt ttacccgatc accattacga gcagaaataa
    22081 aatgaggaag cagcatacct tgatcattcc gtacctcgaa ataacgttga tgttctttca
    22141 tagaagtgac tagtacttct tcaggaacag aaagatattt ttcttcgaag tcgccagaaa
    22201 agacagttgg atattcgact aaattgttga cttcttctag taaatcttcg tctagatcaa
    22261 taacccagtg atttttttct gctaattcgt ttgcttgtgt aacgatcatt tcttgtcttt
    22321 tttgcggaga cgcaatgacg aattgttcat tcaatttttc ttcatattct tttgcatgcg
    22381 caaatgtcac atcttcgcct aagaaacgat gacctcgaga ggctctgcct gttttgacat
    22441 ccaagatttc gaatgggatt acttcgtcat ctaacaatgc cacgatccag tgaatagggc
    22501 ggatatattc aaaatcgtat tctgcccaat gcatcgttac agggaaagtc aaacttttga
    22561 tcacccctgc taagcctgtc aatacttctt ttgcttcctt accatgagtg tatttcgtta
    22621 catagacata ttccacacct tttaattctc taaaagtgat agcgtctgtc gtcagacctt
    22681 gtccgcggac aaatccttcc gctgctttcg tccagtttcc atctgcgtcc aaagcaattt
    22741 ttttggctgg tcctttgact tcttcttcac tgtccgcttg tcgttctgga atatttgtta
    22801 ctttgattgc taaacggcgc ggtgtggaaa atgtttcgat cgtatcataa gtcaattggt
    22861 tttcatctaa aaattttgct gttttttctt ctaattgttt catgcttggt gtgacaacat
    22921 gggcaggcat ttcttccagc ccgatttcaa gtaataagtc ttttgccatc ttattcagcc
    22981 tccttcacgg tttctggatt tgctgtgttt ttattcaaca aagggaatcc taatttttcg
    23041 cgttctgcca caaagatttt ggctacagca cgagccatgt tgcggatgcg agaaagataa
    23101 ccggcacgct ctgttacgga tactgcaccg cgggcgtcta ataagttgaa tgtatggcta
    23161 catttcaaga tataatcata cgccggatga acaagatttt catcgataca gcgtttagct
    23221 tctttttcaa aacgatcaaa gttgcttagt agcaagtctt ggtcgcttac ttcaaaagaa
    23281 tatttggaat gttcatattc tggttgtttg aagatctcgc catatttcac gccatcagcc
    23341 cattctaaat catagacact ttcaacttct tggatataag aagcaagacg ttctaatcca
    23401 taagtgatct cagaagttac tggtttacaa ggcagtccac caacttgctg gaagtacgtg
    23461 aattgtgtga tttccattcc gtcaagccat acttcccaac ccacaccggc acagcccatg
    23521 gatgggttct cccagttgtc ttcaacaaaa cggatgtcgt gttctaatgg atcgattcct
    23581 aaaaggcgca agctttctaa ataaagctct tgaatattct caggtgaagg tttcatcacc
    23641 acttggaatt gatgatgctg atacaaacgg tttgggtttt caccataacg tccatcagct
    23701 ggtcggcgag atggctctac gtaagcagca ttccatggtt ctggtccgat tgcccgtaaa
    23761 aaggtatatg ggctcatcgt acctgctcct ttttctgtgt cataagcttg catcagcatg
    23821 cagccgtttg atgaccaaaa tttttgtaat gttaaaatca tatcttggac ggtcaatttt
    23881 ttactcatct gttttgcccc tttcaatttt aaagcgttgc tgtcaaataa gaatacagga
    23941 aagaaaacga caaaaaatcc ctatgtctga ttacagacat agggacgaat aattcgcggt
    24001 tccaccctac ttccgaataa agattcggca gcttcattta tcgtactcgc aagtgcctct
    24061 tcaaaaatcg tttgggtccg gctcccactc tcccgaactc gctaaaccac gtgtttttta
    24121 ctgatcttgg tcaatgtacg tattcatttt gcagttttct tgtttctata atagatccta
    24181 tcactgaacg atctatttaa tctatggtta ataatagacg gaactccctg tgtttgtcaa
    24241 atggttttga ttttttgttg tatgttttat ttactattct tctttttcag gtgtctcttt
    24301 ttctttttca gacatgtctt tctctgacgt ctttaatacg tgtgcccaag acttcatttg
    24361 attgatgaac tttttgcttt tcaaatggat accgacatat tcatcataca gctggtcgat
    24421 gagttcacgg attgcttgtt tggtttcttc tttcagatgg attccactaa ttttatcata
    24481 agaaatagct gaaaacatcc gaacgaaata aacagctcga ggatctgcat gataacggtg
    24541 atcgtccatc gaccaatgtc tttcgcatat cacaccacca tatttggaag aatagtcaaa
    24601 ttttcctttt gtttccccgc atacgacgca gtgtgtccag attggtgcaa ttccgaagcg
    24661 ttgcagtatt tggacttcaa aaatattcgt gatgatttct gcatcgtctc cttggtccat
    24721 ccgttgcagt gcttgttgcg taaactggaa caaatgcggg tcgtactgat gatcttcgat
    24781 tgccgcatct actagattca ataaatatgt gccatagccg ctcaaaaaga tgtcttcttg
    24841 gatgtgtcga aacgcatgga catctttgac actgcttaaa aatgacaaac cttcacttcg
    24901 aaaattcccg atataaactg cttcagtaaa agggagcaaa gccggagcta agggctgatt
    24961 ttgccgatgg atccctttga tataaaacat cattttgcca gaagattccg taaagatttt
    25021 gactaattta tctttttctt tgtaattacg gctagataaa atcaagcctt ttgattctgc
    25081 aagctggctc atattctccc tcctaaaaaa taaatctgga aaagcagatt ctgccgatcc
    25141 agatcattta tgttgcgatc ttccgcagct tttctcaaca cgcggatttt tatccagttt
    25201 atttctatac ctaacttaca agaagctcag aatatcaata ttcgtctttt cggtagccga
    25261 agtcttgcag atagactttt ttgtctcgcc agtctttttg aactttgacc caaagttcta
    25321 gaaagacttt gtctcctaaa agattttcga tatcttttct cgctttcgtt cctacttgct
    25381 tcagcatttt tccgcctttg ccgataatga tccctttttg gctatctctt tcgacaataa
    25441 tcgtagcctg gatatggatt ttatcattct cgtttcgctt catcgaatca acaacgactg
    25501 cgacagaatg aggcacttcg tctcgtgtta aaaacaagac tttctcacgg atcaattcag
    25561 aaacgatgaa gtattcagga tgatcagtca cttgatcatc tggaaaatat tgtgggccct
    25621 ctggcatttc actgactaat acatccatca aacgttcgac gttgtttcct tctgtagcag
    25681 aaattggaat cacttgtgca aacggcatct gcgttgaata atcttcgatg attcccaaca
    25741 actcgtctgg atggatttta tcgattttat tgataatcag ataaacagga gaatgcacgt
    25801 tttttagacg ttcaataata aaatcgtctc cccgaccgcg tttttgatca gcgctgatca
    25861 taaataagac cgcgtccact tctttcaacg cactgtaagc tgcttctacc ataaaatctc
    25921 ctaaacgatg ctttggttta tggattcctg gtgtgtcaat aaaaatcaac tgagcttctg
    25981 gtgtagtata tactccttgg attttatttc tagttgtctg cgccttatca ctcatgatgg
    26041 caattttttg acccacgatt cgattcaata atgtggattt tcctacattc gggcggccga
    26101 cgattgcgac aaaccctgat ttatgttctg gcatatacgt tctccttttt aattaaacaa
    26161 caaatgatat attttaggga caaataaaat caacccaacg atgacggaaa aacaagcggt
    26221 cactaaaact gctccagctg ccatatcttt gatttttttc ccaatcggat gaaaatgaaa
    26281 atccgtaaac atgtccacga cattttcaaa aacagtattg atgatctcta ctatccatac
    26341 gagaaaaaca gctaaaagca gccataacca ttctatccga tcaagctgga aaatcacgcc
    26401 cataaacaaa gcgaggattc cgaacgcaac atgtttcctc atgttgcgtt cttcctcaaa
    26461 aacggttttg actccttgga cggcaaattc cacggacatc aggaaatgtt tgtttttctc
    26521 tacgttttta tcttttaagt ccataggcat ctaagatctc tttctgtaaa ccaaacattt
    26581 ctttctcatc ctctggtgtc atatggtcat acccgttgat atgcaagaat ccatgaaccg
    26641 ctaaaaagcc aagttcccga tcatacgaat ggccgtaagc ttctgcttgc tctgcagcac
    26701 gttcagtaga gatcatgatg tctcccagta cacgaggcaa tggattctct ccatcttcaa
    26761 aaatcactgg gatttcgtct tcgccctctt cttccaaggc aaaactgatg acgtctgtcg
    26821 gtgcatcttt tccacggtaa tcacgattga ttacttggat cgctgcattg tccatgaaag
    26881 taacagacat ttctgtttct tctggcaact ctaaatatga tgctgcaaat tgcagcaagt
    26941 cgtcgatttc tttcattttc tcttctgaga cttggtttgt ttcatccatg aaagtaatat
    27001 ccattataca tcgcttcttt ctattcttct tgttcttctt ttatcttctc aacttccgat
    27061 ttcattttag gatatttgat ccgagaatgg aaggtactgc ttaaaccgct gatcaatgat
    27121 ttttctactt tacggatttc ttttaacgtc aatccactgt cgtccaattg tccatcggaa
    27181 atacgctctt caataagatt atggacaaaa ctttcgattt tttcgctcgt tggatggtcc
    27241 attgctcgta ctgccgcttc acaactgtca gctatattga cgattcctgc ttcacggctt
    27301 tgaggttttg gtccaggata acggaattgc tcttccgtga cttccgggtt gcgttccttt
    27361 gccttaacgt agaaataacg catcagcgtg gttccatgat gctgtctgca aatatcgatc
    27421 accatttgcg gcatattata atcttccaat attttggcac catctgtcac atgtccaaag
    27481 ataatatgtt tactgtcttc tggcaataag aaattgtgcg gattttccgc tcctgaaggc
    27541 aagttctcaa cgaaaaagct ggcgtgtttg atcttgccga tatcatgata atagcaagct
    27601 actcgagtta gtaaagaacg tccaccgatt tctgctacag cattagcact tagattggct
    27661 accatcatgc tatggtgata cgtgcctggt gcttcttcca gcaactgttt caataaagga
    27721 tgattaggat tgctgagttc atttaatacg atcacactat catcattgac catcaattca
    27781 atataggggt gcagacctat cgtcagcaag aatgagaaca gtgcacctgt gaatccgcat
    27841 actagcgtta accaagtcgt tgcatcccca aagctcattc cttggtagat aatcaaaatg
    27901 acatccatca tcacaggaaa aataatgacc cacatagcag cggataaccc ttgttctgag
    27961 atccgtttgc gtctgacgat cgttgccatg agaccagaaa atacatagga catcaaaata
    28021 actgtcagaa agttcgtgcc aatcgaatca taaaaaatga aaagtgcgga tactgcttga
    28081 aaaataccag ccatgatccc tgctcttcga ttcaagaaaa agcttaaaac aagtggagca
    28141 aaagccgcgg ggtaaaacag cggaatataa gcagcttgtt ctgtttggaa gagttggaaa
    28201 aatttcatta gtacgacgct tactgacatt gccgtcacgt agaaaagtac gtattttgtt
    28261 ctttctcctg cattttggaa ttgaagtgaa ttataaatca gtacaccgat ttgaagcaaa
    28321 acagctaaaa ccatggcaat gagtgggaaa atagaagtcg tttgattcgt caatcctagt
    28381 aattccaatt ttttcatcgc tgaagcatca atttgatttc cttcgcgaac aataatttct
    28441 ccttggaaga tcatcactgg ttgtacagct tctcgtgcag attgttttaa ttcttctgtc
    28501 tttttctcat tcaatacatc attaactaca atgccttgat ccactaagta gcgaatcatt
    28561 tgttgctgct ctgttgtgac attcaaatat tgaatctgat cttctgcttc ttgtttaaaa
    28621 gcttccaatt cgctttcacg aacttgttta ctcatctgtt cgtcaatgag cttcaaactt
    28681 tcatccctaa ccgtgtgaat ttccgtttca gtcatttcaa atatcgtcga ataaaaattg
    28741 ttcggcagtt tttggtaaaa cgcaacgttt tctgcattca cgttttcaaa atttttcttc
    28801 agtgtagcaa tccgctcttc tgtagtaggt ttcgggatat tttcatcttc cttcgcctta
    28861 tctaccttgt cattataaga tttgttgatg gtatcattcg tcttatcgat caattcgaat
    28921 aattgcttga tccgattgtt ctggtcatct gccagatctt tttgataagt atattctgga
    28981 gtcactgctt ctgctgccag ttttcttctt tgttcggttt cttccgtgtt ctcaatcgtt
    29041 ttattcgcac ggatactctc ttctgctact tggccttctc tgaaatcatt ggccttttgt
    29101 ttgacactac taaatgtaac gccgcagaca atgataaaaa acaagaacag aaggaaaggc
    29161 aatgctgcct ttccgagttt tttttgtagt ttctgcagtg ttttattcaa catgtgctta
    29221 tcctcctaag caaagcttat tcttgatgaa gatccgcctt ttcataagct ttgatgattt
    29281 cggcaactac agggtgccga acaacgtcac ttgcttcaaa attaacaaaa tcgatttttt
    29341 tgatttgctt taatgttcgt tctgcatgaa caagaccact tgtcgtccca cgaggcaagt
    29401 ctatctgact tgtatcccca ttgacaatca tttttgattg ataaccgaga cgggtcaaga
    29461 acattttcat ctgagcgact gtcgtgtttt gtgcttcgtc caagataacg aatgcatcat
    29521 ccaatgtccg cccccgcatg taagctaaag gtgcgatttc gatcacgcct cgttccatca
    29581 atcggttcgt atgatccaat ccaaaaattt ggtataaagc atcataaaca ggccgcagat
    29641 aaggatccac tttttctttc agatccccgg gtagaaagcc taggttttcc cctgcctcca
    29701 cagcgggccg cgtcaaaatg attttttgca cttcgccttt ttttagggca gcaattgcta
    29761 atactacggc aagaaacgtt ttccctgttc cggctggccc tacaccaaat acgatgtcat
    29821 gtttggctac cgattcaacg tatttctttt gaccgctgtt ttttacccgt ataggttttc
    29881 cattacggtc tttgacgatc tcttcctcat acatctcaac aaaataatct aatgtacctt
    29941 tttgccccat cttcaatgcc gtcacaacat ctggcgtact tacgtggatt cctcgtttga
    30001 tcaattcctg taatgtccga atgactgagg cagccaagga aatctgggat tgctcaccaa
    30061 tgatctgaat gatttctcca cgtgtatgga tggttgtttc cgttgtttcc tcgatcagtt
    30121 ttatatgctt gtcgtgtgca cctaaaagca tgctgacatc atcagcagct gtcaatctaa
    30181 tctctaaaga actagactct tccgtcaaaa atagacatcc cctttttttc gattcattct
    30241 ttcctatttt accatactca gaagaaacta tatagcaaat aagaaaaggt tgtaaaagag
    30301 tcgtttaacc atacgtacca agaaacgtta aaaaatagcc gtttccattc gctaaaggtc
    30361 tcgttttgat aaaaagaagc tgagaatgta agttatatga aagtggagaa caaaatattc
    30421 cgtttcattc agcaggtatt ctctaggtat ttttcttgtt tcaaaaaaag aaaacaaaat
    30481 agttaaaata ttcttgcatt tcaaaaaaaa caggtgtatt attacgtcaa caaataaaca
    30541 tacagcaaag cgtcgaggaa aacagtaggc tattcaacga tttattccca gtgagtcatg
    30601 taagtgagaa atgacaataa atcgaataga cgaacatcat ttccgagtca atgtcgaaaa
    30661 cgtagatcat tgtggctgcc tccattatca ggctagcgga taactttatc aaaaagcgtc
    30721 cgtgaatgaa gaagcttttt taagcgaaaa aaggtggtac cgttctagaa tctagagccc
    30781 ttttcccaag tggaaaaggg ctttttttat attttaaggt ggtggttttc atggaatctg
    30841 atattagtgg cattactaat tgctgcaacg tttatatcgt caatcaaaat gataattatt
    30901 ggaggaaaaa tttatggaag aaacaaaaac ttattcatgg aaacaactat tgatggtcag
    30961 ttcacttatc ttcgggatgt tcttcgggtc aggtaatcta atctttcctg tccacttagg
    31021 tcaaatggct ggcgcaaact gggtcagtgc tggcgtagga tttgccatct cgggcgctct
    31081 tttcccatta ctagctatct tagctgtagt agtgacaaaa agtgatggtc tgtatgactt
    31141 agctaaacct gtcggaaaat ggtatgctgc cttgttctta atccttgttc atttgacgat
    31201 cggaccattt ttcggtacac cacggacagc tgctactgct tttgaaatgg ctgctaaacc
    31261 atttttgcct gaaaaatata attcattagg tatgttttta ttctctgctc ttttcttttt
    31321 acttgcttat ttccttactg tccatcccac acggttggtc aaatacgtcg gaaagtatct
    31381 aaactccttg tttcttgcat tgttagcaat catttttgtc gtttctttat tccacccaat
    31441 gggaaatcta aatcaatctg ctgcagatac ttaccaaaca aacgctttaa caaatggtgt
    31501 cctagaaggt tataacacgg ttgatgcagt agccttgctt gccttaagtg tcacttttgt
    31561 ccatgcagtc cgtgaccttg gtttcaaaga taaaaaagtc acagaattaa cagcaaaagc
    31621 tggtagcctg agcgttgcgc tagaagtcat gatctatttt ggtcttgttc ttctcggtgc
    31681 gatgagtttg aataagatgt ctttatcaga aaatggcgga actgcacttt ctcagatcac
    31741 taatttttat ttaggtaact tcggcgctag cttcttagga gtgatggtca cattaggtgt
    31801 cttcactacc gctatgggac tcgttgtttc ttttgcgcaa gatttccata aactgttccc
    31861 taaagtcagt tatatgacat ggctgcggtt aacgactttc gtctcttttg tcgttgcaaa
    31921 tgctggttta gacaatatca tccaatggtc tttaccagta ttgatgcttt tgtatccatt
    31981 gtcgcttgcg ttgatcttgc tttccttaac agcaaaattt ttccaaaaga caccatttgt
    32041 ctatcaagta acgatgttgt ttgctgctgt tcctgccgta ttggatatgc tggcaaattc
    32101 gcctgcttta gtgagtcaac aaagagttgt agcaagcatg ctcgaatttt atcatcatca
    32161 cgttcctttt gctgcattag gacttggctg gatggtccca acgttacttg gttacgctgg
    32221 cagcctactg ttttattacg cctatcgcct atcagggtat aaacaagaag caaatgaact
    32281 accagaagaa taaccaaatc aaacacaatt taacataaga aatattaaac aggaaaaaac
    32341 cagctagatg aaagaatcct attctttcgt ctagctattt ttttgttctc ttttttacgc
    32401 gcacaaaaaa actgcggaca atgattgtcc gcagtttaaa tgataaaatt aagattgtaa
    32461 tagttctttc acaatggcat ttacttggtt tccatctgct tttcctttga ctttaggcat
    32521 gatcgcaccc atgactttgc caaattcttt cattgatgaa gcaccagttt gtgtgattgc
    32581 ttcttgaaca agttgtcgaa tttcttcgtc ggaaagttgt tccggcatgt atttttcgac
    32641 tatcacaatt tcacttttta ctttttcagc taaatcgtca cgtcctgctt cttcaaactc
    32701 atgcaaagaa tcacgtcgct gtttcatttc gcgagacaac accgttaatt cttcttcttc
    32761 tgtcaagtcg tgaccttctt taatctgctc gttttgtata gatgacttga tcattcggat
    32821 gacttgtaaa gattccttat ctttcgcttt catcgctgtt ttcatgtcat cgtttaatct
    32881 acttagtagt gacacactac caactccaat tcatcacaga actagaattt tttgcgtttt
    32941 ctagctgctt cagatttttt cttgcgtctt acacttggtt tttcgtagaa ttcacgttta
    33001 cgagattctt gtaaagtacc cgcttttgac acggaacgtt tgaagcgacg aagagcatcg
    33061 tcaagtgatt cgtttttgcg aacgactgtt tttgacatgt taattccctc cctccgagct
    33121 taaaaagtaa ccgtaattgt tattgaaata tatctcaaaa aacaagactg tcctgtaaat
    33181 attatatcta atgctgtttt gaccgtcaag gaaatcttgc ttttttttaa aattatcaaa
    33241 tgttatttta tttaaatcct tattgacaat tttcacatct tccgaaaatc tcgaatctat
    33301 gcccttcgat cgtacatcca ccgagctgtt cttcgaaata attcattgga cacatatgga
    33361 tttctttcgt tgttccgcac accgtacaga taaagtgatg gtgatggccg atttcccctt
    33421 gacagcaatg aaaacgaaac ttcatctctc catttaaatc ggtttcttcc agtaaatcga
    33481 tttctgcaaa atcatggaga tttcgataaa tcgtatcgta acttactcct ttgaattcct
    33541 gattcatata gtcaaaaact tctttagcgg atacgtagcg attgcgcttc actaaataag
    33601 ttaacaatgc ttctcttttt ttagtatatt taaaaccgtt atttttcaat gtttctagtg
    33661 attgttcaat caatgtctgt tttttcatga cttattcctc caaatcaaaa tgattacgaa
    33721 gtacacattc tagagtatgg tataataaac ataaaaaaaa tgcaagtgag gttttatcca
    33781 tgtcttccga ttctacagaa atcgttactt tttttgtcat ctctgactct gctggagaaa
    33841 ctgcgacaaa attagcgcaa gccactatgg cccaatatcc taccgttgag ttcaatttat
    33901 tccgcagaac atttgtgaca gatgagaaaa cattgatgca ggcactgcaa gatgcactaa
    33961 aagaaaaagc ccttgtcctg cacacattga tcaatcaaga attgatcgag atgacgcgtt
    34021 ctttttgtga agaacatcaa ttatttagtt ttgatgtgat gactccgccg atatcagaaa
    34081 tcgagcgctt aactggtatc aagcccattc ggcaacctgg cgcccttcat ttattgaatg
    34141 agaactattt caaacggatc aaagcaatgg aatttgctgt aaaatacgat gacgggaaag
    34201 atcctcgtgg gtttctagaa gcagatgtcg ttttgctagg cgtatccaga acttccaaaa
    34261 cgcctttgag cttgttttta gctaacaaaa atttaaaggt tgccaatcta cctttgatcc
    34321 cacaagcaca tattcccaaa cagctttggg agatcgatcc taaaaaaatc gtgggactga
    34381 cgaataaccc agatatttta aacaatatcc gtaaagaacg gatgcgctct tacggattaa
    34441 atcctgatac tgcttattcc gatatcgaaa aaatacgagc tgaactcgat tttgcgaatg
    34501 atctttatga aaaattgggc tgcgtagtaa tcaatgtcgc ttctttatct atcgaagaaa
    34561 cagcttcact tattttgaat gctttagacc tagaagatca tagttattat ggaactgaaa
    34621 ccaccgaaga aacagactaa cagtttttct tgtctttttc ttaaaaaaac tggactgtgt
    34681 cagcgaagaa gattcgttga tacagtccag tttgtctcta tttgcaccta tcgttattga
    34741 tgttccacat gtttgagcgt ctggttcaaa atatccagta cgtgagaatc atcaagacta
    34801 tacaaaatcg cttttccctc acgatgggac ttgacgacat ggttttctct taataaacgc
    34861 agttgatgcg atacagcaga ttgttccatt tctactgctt gggtgattgc tgtcacattt
    34921 tgttccccgt ctttcagata cagcagaatt ttgagacgag tggggtcact caacattttg
    34981 aatagttggc tgactttctc tacttttgtt ttttgcgtgt ctggcaatcc attcacctcc
    35041 aacttgaaaa aagactgact tctaaagttt accacttttt ccatcagtct caagtttttt
    35101 tattcttctg tcattttttt gataatcgct tcataagcgg ctactttttc agctgcgtct
    35161 tctgaagagt ttccttttac accgatataa aatttgattt tcggctcagt tcctgaagga
    35221 cggatagcga tccagccatt ttcttccagc gtatacttca atacgtcaga tggcggtgtc
    35281 gttagttttt ctacgtttcc ttcacggtct gttctcgtca actctttgaa gtcttctgtt
    35341 tggatgactt ttgctccggc aaattctgtc ggtgcttctt cacggaattt ttccatcaaa
    35401 gcagagattt ttgcagaacc ttcttgtccg ctcattgtca cagaaatcgt tttttctgcg
    35461 tagtagccat atgtttcaaa aatcgtttgc aagccgtcat acaacgtttt gccttgtttt
    35521 ttatagtaag ctgctacttc tgctaataag agcaatgctt gaatcgcgtc tttgtcgcga
    35581 acgaaaggtt taactaaata accgtaactt tcttcgaatc cgaacataaa agtatgggaa
    35641 tgatcttctt caaattgttg aattttttca gcgatgaatt tgaatcctgt caatacattg
    35701 aacatggtag ccccatagtg ttccgcaatt gctgtaggca attcgctaga aacgattgat
    35761 ttcaataccg ctgcattttc aggtaaagtg cctcgcgatt ggtgtgcttc caaaatatat
    35821 tgagcaaaca gggctccgat ttggttccct gtcaatactt cataggagcc atttggcaaa
    35881 cgaacggctg cgcctaaacg gtctgcatca ggatctgttg caatcagtaa gtcagcgcct
    35941 tctttctcgc caagtcgcat cgcatactcg aaagcagagt gttcttctgg attcggtgat
    36001 ttgactgtag tgaaatccgg gtctgcgaca gcttgttctg gaactagtac gaattgttcg
    36061 aatccagctt gtttcaacgc acgttcgccc aacatttttc ctgttccgtg aagtggtgtg
    36121 tacacaagtt tcaaatcttt acccattgtt tccaccaatt catgatcaat cgttactgct
    36181 ttgatttctt ctagataagc ggcatccact tcttctccga tgatcgtgat caatccgcta
    36241 tgttcagctt cgtcgtcaga taatacttca acttttaacg gattgctgac ttcacggaca
    36301 tatttcgtca atgcatctgc gtctgcagga ggcatttgtc ccccatcttc tccgtagact
    36361 ttatatccgt tgtaagctgc tggattgtgg gatgcagtta ccatgattcc cgcaaacgtc
    36421 ttcaaatgac ggactgcaaa agaaagctct ggtgttggac gtaaactttc gaatacataa
    36481 gaagggatat tgtgatgtgc taatgttttg gctgcttcca tggcaaattc tggtgattga
    36541 tggcgtgagt cataagcgat agcgacacct cggcgttttg tttcttcacc ttgtgtatcc
    36601 atgaaacgtg caagtccttc tgttgcttgt cttaccgtat agatgttcat tcgattgatc
    36661 ccaggtccta agattccccg catgcctgca gtaccgaatt ccaacggtgc ataaaaagca
    36721 tcttcaagtt tttcaggatc ttcacccaga tcaattaatt gtttttttaa gttttcttct
    36781 aaattttctt catgtaccca ttgttcatac acttgttccc aagacatgaa aataacacct
    36841 ctttctgaaa tatggttctt aataagtata gcattcactt attagatatg aaagtacgag
    36901 gtctaaaaaa cgcttaaatc aagaaaggat tcttttatca agcacggttg caagtattcg
    36961 cataaatcgg attcagtaga tttcacgcga aaaaaaagag taaaagaaca attgaaaccg
    37021 ttcttccact ctttttaaac ttatgcagtt gtttcttcta aagattccaa gtatttatac
    37081 acttgttgtc cagcgatccc accatcacca acagcagtag tgatttgtcg caaatctttt
    37141 tcacgaacat ctccgatggc aaaaacgcct ggtatattcg tcttcatttc gttgtctgtt
    37201 ggaatccagc cagcttcgtt cgtcaaccct gcttctctaa atggtgcagt taatggatcc
    37261 agtccaacat agatgaatac tccgtctgct gccaaatcaa actcattgcc ggttttgaca
    37321 ttacgtgctt tcacacctgt cactaccatt tcattcccaa cgatttcgtc aacgactgta
    37381 tcccacataa aggaaatttt ttcattcgca aatgcacggt cttggatgat cttttgtgca
    37441 cgtaattcat ctctacggtg gacgatgact acttcagaag caaattgtgt caaatagatg
    37501 gcttcttcta ctgctgagtc tcctcctccg atcactacta gacgtttgtt acggaaaaac
    37561 gcaccatcac aaactgcaca gtaagaaacg ccacgtccag caaattcttc ctcgccaggt
    37621 acgcctaatt tacggtggat acagcctgtt gcgataatga ctgtttttcc ttcgtaagtt
    37681 ttatcttcac aaataacttc tttgtggctg ccgtgatcct tgatatcttg cacgattcca
    37741 taagcatttt ctgtcccaaa tcttgaaaca ccttcataca tcttataagc tagatcagga
    37801 cccatgattg aatcgaaacc aggatagttt tcgatttctg ccgtattgtt catttggccg
    37861 ccaggggcgc cttgttcgat catcaatacg gataagtttg aacgtgaagc atataaagca
    37921 gcagtcatcc cagcaggacc tgcgccaata acgattacat catacatatg tagattctcc
    37981 tttacttaat caagtaagca tactttacag ccaatgcgaa aaaaagtcta ttgttctgct
    38041 tgcgtttctc gaatagcttt attatactcg aaaaatccgc tgaaaaacaa tagactttta
    38101 caaatcattg atcaatcatt cgaaagattt tctccatttg aatcaattac ttttttatac
    38161 cagttgaatg atttcttctt cgaacgggct aatgtgccat ttccttcgtt gtcacgatcg
    38221 acataaatga atccgtacct tttcttcatc tcacctgtac cagctgatac taaatcaata
    38281 catccccaag tcgtatagcc taatagatcg actccatcta attctactgt atctttcatt
    38341 gcctggatat gtgctgccag atagtcgatt cgatagtcat cttccacata accattctca
    38401 tcgggcgtat cgactgcacc taagccgttt tctacaataa atagtggttt ttgataacga
    38461 tcataaatat cattcatcgt tattctcaag cctaatggat caatttgcca accccattca
    38521 cttgcttcca gatatggatt tttgacagaa gcaaagatat ttcctgcagt ctgttccaag
    38581 agttcgttgt cagtagtcga aactcgagaa gaataataag aaaaggagat aaaatcaaca
    38641 gtatgttctt tcagcaatgc ggcatcccca ggttccattt tgatctgaat gccatttcgt
    38701 tccatttctt tcaatgcata agcgggatat tctcctcgtg actggacatc aatgaagaaa
    38761 taattttctc gatctgattt tctagaagcc caaacatctt caggattgca agtgtatgga
    38821 taatatgctc ctgctgccag catacagcct actttgttct ccggatctac ttcatgagca
    38881 atcttcgttg cgatggcact tgcaaccaac tcatgatgtg ccgcttgata tttgatttgt
    38941 tcttcatttt ctccttcttc aaaatacagc cctgcgccca tgaatggtgc atgaaggatc
    39001 atattgatct cattgaatgt caaccaatat ttgacaagtc ctttatagcg attgaagatg
    39061 actcggcaca gattttcata aaaaccaacg agttctctag aacgccacgc accatattct
    39121 ttgatcaaat gcatcggaca atcaaaatgt gtaatcgtca caagcggctc gatcccgtac
    39181 ttttgacatt ctttaaagac atcctcataa aatttcaaac cagattcatt tggctcattt
    39241 tcatcgccca ttggaaaaat acgactccaa gcaatggaca tccgataagt cttgaacccc
    39301 atttctgcaa acaaagcaat atcttcctta taatgatgat acatatcgat cgcaattttt
    39361 gctggataaa aatgatcttt atcaaattca aacattttct tttttccagt aatgacagga
    39421 aaacggtctt ctccgattgg cacaacatct acgttcgcta acccacgtcc gccttcatca
    39481 taaccaccct cgcattgatt ggctgctgta gcaccgcccc ataaaaaatc ttttctgaat
    39541 cccatcatac ttcctccatt acatgtgtat tgcttgacaa ttctcttgcc agttcatctt
    39601 ggacttcgat tccttctgct tttaaccggc tgattgtttc cttgaattgc ggtaaatact
    39661 gctgatgtgc aataaacaac tcatccatga tccgttttgc tgtctttcct gatcgtataa
    39721 gcggattgat cgtaaacgct tgtaaagctg ttccataatc accagtcacg gctgcttcga
    39781 tcgtcagcaa ttccatattc ttcattactt gcaaccagcc tctttcagca gcaggaaggg
    39841 aaccaaacgc tacatttcgt gcgccttgtg cgccaacata ggcagtgact tctactacac
    39901 aatctgccgg cagatcagga acagctccat cattctttgt agacaccacg atctgtgttt
    39961 ctttatttcc atagatcgaa gcaatcgttt cacaggcagc atctgaataa taagcaccac
    40021 ctcgtttttg caattgttca ggtttatgat ccaattctgg atctttgtat aattcaaaca
    40081 gctctgcttc tgtttctttc acttgttgcg ctcttgtacc aatcgtttga tattcttcta
    40141 aagcatgttc caacatttct tcttcacggt agtaatagcg gtggtagcca caagggatca
    40201 tgttcatttg ttttaattgt tctttgaaaa acgggatgtc aaagatattt ttcggcaatc
    40261 cattgtcttc atcgtataaa cgatcaatca aatctgctgt gcggtcattt cctttattat
    40321 ctgctacttt gtgccaatgg aagtgattta gaccggcaaa cttgtagatc agctcttctt
    40381 ttttcttgcc aagcattgct ggttctgtca tcgtcgccat tactggcaca ttgcataatc
    40441 cgatcacttt atcccatttg ccatatctga taactgcttc tgtcaccatc ccgcttgggt
    40501 tcgcgaaatt gatcagccaa gcatttggac ataaacgctt catgtcttca acgattgcca
    40561 aaaccactgg aacggtacga aaagctttga atatcccgcc agcaccattt gtttcttgcc
    40621 caatcatccc ataataacta gggatccgct cgtctttgat tcgtgcatct aattgaccga
    40681 cacgaaactg agtcgtaaca aaatccgcat ctttcaatgc ttcttcccga tccaaagttg
    40741 catagatctt gacttcatac ggagaagcat cccacatacg ctgagacatc tgactgacga
    40801 tctcaagctt ttcttttcct tcttctacat ctacgagcca gatttctcgt atcggtaatt
    40861 cctcatatct tttgataaat ccttccatca actcgggggt gtaactgctg cctccaccaa
    40921 tcgtagcgat tttgattcct tttgccatgt tctcactcct ttgcttttta cacttttatt
    40981 atcttttttg taaacaaaac aaacaataga aattcgtttc tttccagtaa catttacaaa
    41041 agttagtgtt ttctactgga tttttgtaaa tatcttgtca aaaaatgact ttattaagct
    41101 atataacata aatccgttta caaatcattt tcaaaaaagt tataatttac gtaaaaaaac
    41161 tagaagaagg tatctacatg ttattacgtg aaaaaatcaa agaaatctcc ttttctcctt
    41221 cagaaaatga agtgattcag tttctcttaa gcgattctcc agcacttggc gaaatgacca
    41281 tccaagagat tgctgagcga tgttatgtcc acccgtccac actcatccgt gtagcaaaaa
    41341 aaatgggttt tgatggctgg gtcagttttc gtaatgcttt tttagctgaa acagcctatc
    41401 ttgaagggaa ttttcaagac gtagatgcga atcttccttt ttccaccaca gacggtatta
    41461 tgacgatcgc aaataaaatg gcttctttag aacagatgac gattcaagat acactttctc
    41521 tcattcatca tgaccaactg caaaaagcca aacaactaat gcttcaagca aaaaaaatcg
    41581 tgattttcgc cagtaactcg aatactttga tttctcaaga tttcatgctg aaaatgaagc
    41641 gcatcaaaat ggatgtcacg attgtcacca cttttggtga atcgttctat gaagcgtaca
    41701 attgcacgag tgatatgtgc gggattctga tttcttatac gggtgaaaac aatgtgatcc
    41761 ttcgtaccgc agaaatattg aaacaaaatc atgttccaat tttagcgttg accagtatcg
    41821 gtgattcaac gctatcctca atgagtacag ccgctttgcg acttacgaca cgcgaacgac
    41881 tgtattcaaa aattggtaat tttacgatca atacatcgat ttgttatctt ctagacgtgt
    41941 tatatagttg tgtttttgca gaaaattatc aacagaattt agtccatcta atttctgtag
    42001 gacaaaaatt tgataaacgc cctatcagct cagccatcat gaaagagcct ttcaattttg
    42061 aaaataaaga agtggactaa aaagaatagt taagtctatt ccattctttc acctaaccgt
    42121 tcaggtcagt gcggtgataa gtgcatcgtc tggcccgacc gtctcccctt gattttccaa
    42181 aatatccaaa taatccatgt aattggtgac gattacaggt gtttctaaac tgtatccttc
    42241 cttttcgatt ttctttttgt cgaagcggag caacagatcg ccttgtttca ctctttctcc
    42301 ttgctgcacc aagctttcaa aatatttccc ttccaactga acagtatcca atccgacatg
    42361 aatcagcaat tccagacctg tttcagaaac cattccgatt gcatgtttcg tggggaacag
    42421 ggtcatgact gttccgtcaa aaggggcacg tacttctcct ttttcagggt agatcaagat
    42481 tcctcgacct agtgttcctt gtgcaaaagc tgcatccttt gctgctttca gtggagcgat
    42541 ttgtccttgt atcggtgttt ttacaatttc tttacctgat cttttctgtc tgtcatctgt
    42601 ttcacctgct tcctccttcc aaaagaaaaa agtcagcgca aaggatacca ccatcgcaat
    42661 ggcgatccca atcaatacat taatcacatc tgttaaatca ccatctggtc taatatagtt
    42721 gggaattgta aagatgccta gtccaccttg attccaagaa gttaaattca ttaacccact
    42781 atacgcgccc cctaacgcac caccgacaca agtaaaaata aatatttttt tacgtggtaa
    42841 tgtaaaccca taaatagctg gctctgtaat ccccacaata ccagaaacaa tggctggtat
    42901 tgcaattgct ttgtcttttg gatttctaag cttaaagtag agtgcggtaa gtgctgcagt
    42961 ttgtccaaaa gacactactg caacaggtgt cataatattt tgccaaccat tcacagaaaa
    43021 ttgcatcaat cccattggta cgattgccca atggagacca aacatcacca gaatttgcca
    43081 gaagaagcct acgattgctc cataaataac tggcgaaagt gctaaaagtg tttcaaatcc
    43141 agccattaaa atatcagttg cgaatgtgaa tactggtcca acgagtaaaa aacctaaagg
    43201 cattgtaata agaacggtga agaatggaac gaagaaattc gcgatcatct caggtactaa
    43261 ctttttaatt ggtttctgaa tttggctagc taacaacaca ataaagataa tgggaatgac
    43321 agaagaagcg tagttacgcg caatccaggg aatgccgaat actgtttgat aaataggtgc
    43381 ttcaaaaata gtgccagaaa atatggtcgt tagtggtgct tctgctgcac cactcaaagc
    43441 ttccagcgac aagctaggat tcattaatgc tgcgccaagc aacatcccta cgaattcatt
    43501 tactttaaat ttacgggcag ctgttaaacc gataataaca ggcataaatt ggaatacaga
    43561 atcaccgata ttatcaaaca taacaaatgt gccagaaccg cggtcaaacg cgcctaagaa
    43621 gacaaggaat gctgtcagtc cacgaatcat cccgccagca gctaagatag caaggaatgg
    43681 ttggaaacag cccgatattc catcaataag catatcaaac ggtctcattt tttctgttga
    43741 aacagcctca tcaaaagtaa ggtttcctgc ttttataacc tcttcaaaca cgactggcac
    43801 atgattcccg atgacaactt gatactgccc tcccgctcgc attaccgtta caataccgtc
    43861 catatttttc aagatctcat catttgcttt ttcttcgttt tttaatttga aacgaagcct
    43921 tgtgatacaa tgtgtcaaac cattgatatt ttccttccca ccgacatttt ctacgatttt
    43981 tctcgctaat tcttggtatt ttcccatttc tattccctcc aatatattag tggaaggttt
    44041 agccaactga atgtcactac ccttatttca tacgttcttg ttaatttatc ctcctttttt
    44101 tatttattta caacgattct catgcactaa acgcgcagta tggatcgtta aatacatttt
    44161 ttcgtccgtg gacatctggt agttgaacgt ctcggctaaa aagtctgcta ttttctcaac
    44221 gcaaccaaac gtatccttat attttttttg aataacctga aacaattcct cagaaacttc
    44281 tcccacttct gatttttctc catctaggac gcgtgtcaga aaaaaacgta agtgtgtgac
    44341 gaatcgaaaa aaatggactg aatcttcatc aaatgtaatc ttatagtaat agcggacgat
    44401 atttagaata tcctgtattg ttatagtaaa caggtaggta ttgttttgtt ggctttgaat
    44461 gccttgtgca ttgactaaat gcaatgcgat aaatccggct tcatcttctg ataacttgat
    44521 gtggtattta tccgagattt tcttcactgt ttttttcccg atggcatact ctttaggaaa
    44581 tagttgtttg atatcataca gcaatacatt tggtacaaat attcccattt tgtgacgttc
    44641 gatagccgta tacaaatgat ccgtcaatgc aatatacaca gaatcattca actggacttg
    44701 tagagcctct tttgtatatt caatgatttc acaagaaatt tctaagtact caagaggaat
    44761 cgcttgtgcc aattcttgga actgttgtga aagcccgtca tttgtcaact gaaatttttt
    44821 gtcgatgcat gagtcaaaaa tcgggtcacc cactttcttt ttgaatgcca aaccttttcc
    44881 catcacgatc atttcctgat ttctttcatc cctggttatt acgacgttgt tattcagtac
    44941 tttctcaatc agcatgtttt gctcctttcc atgatatata gggctgtatt caaaacaaaa
    45001 agaaaaaccc aaaaagccgt ccatggaacc atgggagact catcaggttt agcttgcatc
    45061 aggcaatcac tatccaagtt gtatcgtttt ctttatatta tcgaaagcaa acgcttttgt
    45121 caacgctttc ttagtggcta ttaggcactt ttgactagct gtcgtcgtct tttttaaatc
    45181 gggtgactac gccactaacg ctcacagatg aagaatctgg gaagctctgt tttattttca
    45241 ctctagcgct taggacaaaa tgctactatg ctactgtcac aacctctttt tacacaccac
    45301 tgctttgcag tttgtacata tctgcataca aaccattttg cgctagaagt gtttcatgat
    45361 tcccacgctc tactatatga cctttgtcta aaactaaaat caattctgca tcccgaatcg
    45421 tggacagccg atgggcaatc gcaatcgttg tccgtccttg tcgcattttt gccaatcctt
    45481 cttggatcaa tccttcggtt tcagtatcga tatttgctgt tgcttcatcc aatactagta
    45541 ttttaggatc tgtcacgatt gttcgcgcaa aagtgatcag ctgtcgttgt ccactagaat
    45601 agctggcacc gtgttcgatc acttttgagt gatatccttt tggtaattca tcgatgaact
    45661 tgtttgcttg aacaaactca gctgcatggc gaatctgttc gtctgtgata gctggattca
    45721 tcatccggat atttcctgca atatctccat aaaacaagaa ggcatcttgc aagaccaatc
    45781 ccattttctt acgtaactct tccattggat attctcgaat gtcatgatca tcaatcaaga
    45841 tttggccttc ataaaattcg taaaaccgca tcaatacgtt gatgatcgag ctttttccgc
    45901 ttccggtgtg ccccactaat gcaacggtct cacccggatt agcgacaaaa gagatattgt
    45961 tcaatacatt gtgctctcca tcataagaaa agcttacatt gcgaaactcg atcttacctg
    46021 aagtaatcgc ttgatctgct ccaggatttt gttgcggtgc atattcttct gtatccatga
    46081 ttttcaaaat acgactaccg gcaaccatcc catctgtaaa ggtgctcaaa aagtccatca
    46141 tttgagacat tggattaaaa aatccctgga cataggtcgt aaatgcatag atcagtccaa
    46201 cttctacggg agaatgcaac gcatcgatcc caaacaaagt caacgccaac gcaatcgcca
    46261 atgcatagag aaaattgatg atcggactta acagaatcga attcgttttg atcatcgcat
    46321 agcgcgtgtc taaatactcg ttgttcactt gttcaaattc tttagctaaa cgagcttctt
    46381 ggcgaaactg ctggataata cgcatgccgg aaatcgattc attcaatttc gtgttcagct
    46441 gacttaattt ttcccgcata tttcggtaaa tacgagaact gaatttctgg taataccaga
    46501 tgacgattcc taaaatcggc aagaacacca aagtccacaa ggcaatttct ccattgattt
    46561 gaaacatggc aacgaaagaa gaaacgactg caaagatccc agtcaatacc atcaaaaaga
    46621 cataccaaaa ttcaaatagc gtttctgtgt cattcgtcac tcgagaaacg gtcgatcctg
    46681 ccggtgtctg gtcaaaataa cgcataccta gtgtatgcag cttttcaaat aatcttaccc
    46741 gaacatactg ataggtcttc aatgaagcca tggaatacaa ataccattgg aaaaaccaaa
    46801 tgatactttt gactatgact ccaaacaagt acacaccagc aaaaaacaaa ataacttgtg
    46861 tcgtagctga ctgtttagcc agatgatcat ccataaaagt ttggatgatc cttggcaata
    46921 ggacattgat cacagacagg gcaaacgcaa ataagatagc aacgatgaac gttcttctaa
    46981 aaggtttggc gaacttcatc agtcgtttga tgatcttctt ctgttcttta agtggaatcg
    47041 attttgtcca ttccgattga taattttcat ccatcaggcc tcgcctcctt ctatcttcgc
    47101 ttctaattgc tgtttttccc acattcgttc ataccagcca cctaatgcaa gcagctcgtg
    47161 gtgggttcct cgttcaatga ttttcccttg atcgacaaca atgatttctt ttgcatgcat
    47221 cacgctgctt aaacggtgag ctgcaatgat ggttgttttc tcttttctta tttcttttaa
    47281 attatgcaag atagcttctt ctgttttggc atctactgca gaaagtgcat catctaagat
    47341 cagcaattca ggatttttga tcaatgctcg agcaatcgaa atcctttgtt tctgtccgcc
    47401 agataaagag actccacgtt ctccaactaa ggtatcataa ccttttggca attccttgat
    47461 ttcattgttg ataaatgcta aagaagccgc atgctctact tcttcttctg aaagttttgg
    47521 attagaaaaa cggatattat ctcgaatggt catcgaaaac aggaagtgat cctgaggcac
    47581 ataaccgatt gatcctaata aagcattcaa tgaatattct ttgatcgaac gtccgccaaa
    47641 ttggattttc ccttgataat catcgtattc acgcatcagt aatttgatga ttgtcgtttt
    47701 tcccgcacca gttttcccaa caatacctaa tgtttctcct tcttggatcg caaatttgat
    47761 ttgttctaat gctggtgatt cttctttcgg atagttaaaa gaggaaatag ctacattcag
    47821 ctcgcctttt gccggggtct gcactgcacc cagttgttcc acgatatgcg tcttttcatg
    47881 caaaagttca ttaacgcgat cataagaagc atttcctctt tccaatacgt tgaacaatcg
    47941 gccaatcgca aacatcggcc aaacaagcat accgatataa ctaataaatg agaccatctg
    48001 cccaatcgag atcgtgcctt ccaagacata ctttccacct aaaataattg ttgcaacata
    48061 agacagaccg ataatcaagg taatgaatgg atcaaataag gagtctaaaa aattgactcg
    48121 tttgttttta ataattgcat cttcaatctt ttcttcaaaa tccgcgagat cttctttttc
    48181 ttgtccaaat gttttaatga ctttgatccc agtgatactt tcttgtgtct tatcattgat
    48241 actagaaaaa gcttcttgcg aatcacgaaa agcatcgtgg agttttgacc ccagcactcg
    48301 tgaagtaacg gctagcaaag gcagaggaat cagcgcaatc agtgttaagc gccaatcaat
    48361 aaacaaaatc atcgcaatga tcgttgttcc gcccgtaatc actgaatctg caaaggtcaa
    48421 gattccagct cctgctacat tctggattgc attcaaatca ttcgttgcat gagccatcaa
    48481 atctccagtc cgatattttt ggtaaaagag attgtccatt tctgtaaaat gtttgaatag
    48541 ctgctgccga agttctttct ccaaccgagc tgcacttccc caaatattcg tccgccagat
    48601 ataacgaaaa agatattgta agaaagctgc aaggatcaat agtaagatcc accaagaaat
    48661 gatctttatt gataaattct tatcggcgat ctcatcaatg atgatcccaa tcacttttgg
    48721 aggaactaac tgtaccaagg ccactgcgaa tagggagaaa atcccaatta aataatgttt
    48781 cttttcctga cgaaaaaacc aacctaattt tctaaaaatc gacattttac tcctcctttg
    48841 tctttatcaa gagcatagga ttgtctgtcc ttgtcttttt tgctgaatag tctgaatagt
    48901 ctaacaaatt acgaaataaa aaacggactg aaaaaatcag cccgcaaaaa gtctttgggt
    48961 cagaacctat tttttccctt ggttcttcat ttgctgcatc atctggcgaa ttttcttctc
    49021 agatggtttt tgccccattg acatcatcat tgatcgcaac atgtcctcat taacgggagg
    49081 atttttttct aaatagtctt tcatatattt acgcgctagg aaaaagccac ctactgcgcc
    49141 gattagcaaa gcaataatag caataagtac gacaatacct gttgatacca tataaatctc
    49201 tcctttctat ttccgaattg ctctcaatga tttattttac taaagtttat tgaaaaaaga
    49261 aaggcgtatt ctttagtttc atgcaaaaaa tagtggtttt tttagagaag aacaaaattt
    49321 ctttcattaa ccagcaagac acgccacgaa aaaaaattag gcataccgaa tctttttctt
    49381 taattttttc tttctgaggt gtagaatgta atcgtagata aataggaaag gtggtttttt
    49441 cttgaataat caatccaaca aacaatttca taagcatcga cttattgaac atacaaatgg
    49501 tttatcttta gctgaagtaa atggaacagt ggatgtacca aagggaaaaa gtttttttaa
    49561 aacacttctt gcttactctg gaccaggtgc attagtagca gttggttata tggatccagg
    49621 taactggtcc acttcgatca ctggtggaca aaatttccaa tatttattga tgtcagttat
    49681 tttgatgtct agtctaattg ctatgctttt acagtatatg gcagcaaaat taggaatcgt
    49741 gacccaaatg gatttggccc aagcaatccg agcacggaca agtaagacat tgggtgtcat
    49801 tttatggatc ttgactgaat tagccattat ggcaacagac atcgcagaag tcattggtgc
    49861 agcaatcgcc ctctacttac tttttgatat tccattggta attgccgtat ttatcactgt
    49921 gttagatgtt ttcgtccttt tattgctgac aaaagtcggg tttcgtaaaa tcgaagcttt
    49981 ggttgtctgc ttgattttcg tcattttatt cgttttcgtc tatcaagttg gcttgtctaa
    50041 ccctgattgg cacagtttat ttgctggctt tatccctaac agtcatacat tctcagactc
    50101 acctgtcata ggtggtgaaa ctccgctgac tggagcgtta gggattattg gtgcaacagt
    50161 catgccgcat aacttatatc ttcattcagc agtttctcaa actcggaaga tcgatcacca
    50221 agacgaagaa gatgtagcac aagcggtacg cttttctact tgggactcta atatccagtt
    50281 gtcaatggct tttttagtca atgcactgct attgatcatg ggtgtcgctg tattcaaaac
    50341 cggttctgtc caagatcctt ccttttttgg tctgtatgaa gctttatctg atccatcagc
    50401 tatgagcaat ggtatcttga ttgctgtagc aaaatctggt atcttgtcta ctttatttgc
    50461 tgtagcactt cttgcttctg gccagaactc tactattact ggtacgttga ccggtcaagt
    50521 catcatggaa ggattcatcc atatgcgtat cccgatttgg ctaagaagac tagtcacacg
    50581 attgctttct gtgattcctg tattgatttg tgttttgttt acaagtacaa aaggaacgat
    50641 cgaagaacat accgctttga ataacttgat gaatgaatcc caagtttttc tggcttttgc
    50701 attgccattc tcgatgattc ctttactgat gatgaccaat agccaagcgg agatggggag
    50761 ccgattcaaa aattcatttg ttgtaaaact tcttggctgg ttctctgtga ttagtctgac
    50821 ttacttgaac ttacgaggat taccaggtca aatcgaaagt ttcttcggcg agcaagccac
    50881 ctcagctcaa ctagcattgg ctgatcaaat cgcctatgta atcattgttc ttgtccttct
    50941 cctgttggca tggacgattg gtgatttata taaaggaaac aaaaaatatg aacagcgttt
    51001 gcaagaaacg atagaagaaa tcgaatagaa actgattctt ttacacaaag gaggttgtga
    51061 cagaagtggg cagcttcaag aaataagaag gaatttaaga aaattgcttt tcaaattttt
    51121 gtgaaatttc ggcttatttc cgaagaagtt acttctggaa caccgtgtat
//
LOCUS       DACDYJ010000015        49679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDYJ010000015 DACDYJ010000000
VERSION     DACDYJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.015"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="IWM51_000844"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="IWM51_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9970280.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="IWM51_000845"
     CDS             1995..4661
                     /locus_tag="IWM51_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9970281.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="IWM51_000846"
     CDS             5025..5777
                     /locus_tag="IWM51_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9970282.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="IWM51_000847"
     CDS             complement(5799..6953)
                     /locus_tag="IWM51_000847"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP9970283.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="IWM51_000848"
     CDS             7138..9579
                     /locus_tag="IWM51_000848"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP9970284.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9909..10613)
                     /locus_tag="IWM51_000849"
     CDS             complement(9909..10613)
                     /locus_tag="IWM51_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970285.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10800..11045)
                     /locus_tag="IWM51_000850"
     CDS             complement(10800..11045)
                     /locus_tag="IWM51_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970286.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(11062..11913)
                     /locus_tag="IWM51_000851"
     CDS             complement(11062..11913)
                     /locus_tag="IWM51_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9970287.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            12079..13083
                     /locus_tag="IWM51_000852"
     CDS             12079..13083
                     /locus_tag="IWM51_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP9970288.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(13324..13680)
                     /locus_tag="IWM51_000853"
     CDS             complement(13324..13680)
                     /locus_tag="IWM51_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9970289.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            14048..15235
                     /gene="tuf"
                     /locus_tag="IWM51_000854"
     CDS             14048..15235
                     /gene="tuf"
                     /locus_tag="IWM51_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9970290.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(15423..18014)
                     /gene="mprF"
                     /locus_tag="IWM51_000855"
     CDS             complement(15423..18014)
                     /gene="mprF"
                     /locus_tag="IWM51_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9970291.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(18040..18759)
                     /locus_tag="IWM51_000856"
     CDS             complement(18040..18759)
                     /locus_tag="IWM51_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9970292.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            18968..19600
                     /locus_tag="IWM51_000857"
     CDS             18968..19600
                     /locus_tag="IWM51_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP9970293.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            19659..20519
                     /locus_tag="IWM51_000858"
     CDS             19659..20519
                     /locus_tag="IWM51_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP9970294.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            20626..23019
                     /locus_tag="IWM51_000859"
     CDS             20626..23019
                     /locus_tag="IWM51_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9970295.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            23201..25042
                     /locus_tag="IWM51_000860"
     CDS             23201..25042
                     /locus_tag="IWM51_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP9970296.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            25066..26175
                     /gene="rpoD"
                     /locus_tag="IWM51_000861"
     CDS             25066..26175
                     /gene="rpoD"
                     /locus_tag="IWM51_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP9970297.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            26395..28494
                     /locus_tag="IWM51_000862"
     CDS             26395..28494
                     /locus_tag="IWM51_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9970298.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            28690..29553
                     /locus_tag="IWM51_000863"
     CDS             28690..29553
                     /locus_tag="IWM51_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9970299.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            29658..30131
                     /locus_tag="IWM51_000864"
     CDS             29658..30131
                     /locus_tag="IWM51_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9970300.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            30227..31114
                     /gene="xerD"
                     /locus_tag="IWM51_000865"
     CDS             30227..31114
                     /gene="xerD"
                     /locus_tag="IWM51_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP9970301.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            31133..31504
                     /locus_tag="IWM51_000866"
     CDS             31133..31504
                     /locus_tag="IWM51_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9970302.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            31474..32271
                     /locus_tag="IWM51_000867"
     CDS             31474..32271
                     /locus_tag="IWM51_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP9970303.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            32255..32824
                     /gene="scpB"
                     /locus_tag="IWM51_000868"
     CDS             32255..32824
                     /gene="scpB"
                     /locus_tag="IWM51_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP9970304.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            32830..33546
                     /locus_tag="IWM51_000869"
     CDS             32830..33546
                     /locus_tag="IWM51_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9970305.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      33628..33753
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            33876..34487
                     /locus_tag="IWM51_000870"
     CDS             33876..34487
                     /locus_tag="IWM51_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9970306.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     gene            34865..36388
                     /locus_tag="IWM51_000871"
     CDS             34865..36388
                     /locus_tag="IWM51_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP9970307.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            36625..37965
                     /locus_tag="IWM51_000872"
     CDS             36625..37965
                     /locus_tag="IWM51_000872"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP9970308.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            38118..38855
                     /locus_tag="IWM51_000873"
     CDS             38118..38855
                     /locus_tag="IWM51_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP9970309.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(39020..39241)
                     /locus_tag="IWM51_000874"
     CDS             complement(39020..39241)
                     /locus_tag="IWM51_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP9970310.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            39272..40303
                     /locus_tag="IWM51_000875"
     CDS             39272..40303
                     /locus_tag="IWM51_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970311.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            40290..41693
                     /locus_tag="IWM51_000876"
     CDS             40290..41693
                     /locus_tag="IWM51_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP9970312.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            41759..42394
                     /locus_tag="IWM51_000877"
     CDS             41759..42394
                     /locus_tag="IWM51_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9970313.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            42443..43123
                     /locus_tag="IWM51_000878"
     CDS             42443..43123
                     /locus_tag="IWM51_000878"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP9970314.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTREVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            43258..44487
                     /gene="rpsA"
                     /locus_tag="IWM51_000879"
     CDS             43258..44487
                     /gene="rpsA"
                     /locus_tag="IWM51_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP9970315.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            44669..45979
                     /locus_tag="IWM51_000880"
     CDS             44669..45979
                     /locus_tag="IWM51_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP9970316.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            46222..46497
                     /locus_tag="IWM51_000881"
     CDS             46222..46497
                     /locus_tag="IWM51_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP9970317.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            46732..47778
                     /locus_tag="IWM51_000882"
     CDS             46732..47778
                     /locus_tag="IWM51_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970318.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            47780..49039
                     /locus_tag="IWM51_000883"
     CDS             47780..49039
                     /locus_tag="IWM51_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9970319.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            49045..49626
                     /locus_tag="IWM51_000884"
     CDS             49045..49626
                     /locus_tag="IWM51_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP9970320.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
BASE COUNT        16577 a         8600 c        10471 g        14031 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa
     9661 aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa
     9721 ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt
     9781 gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct
     9841 gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa
     9901 tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa
     9961 gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta
    10021 gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact
    10081 tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca
    10141 gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt
    10201 ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca
    10261 gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca
    10321 ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt
    10381 aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc
    10441 ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc
    10501 aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca
    10561 tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt catatgttct
    10621 ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg
    10681 caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt cccgtttttt
    10741 gaaagaaaaa taaaaaatgg aacggattat gtatatatac ataatccgtt ccattctttc
    10801 tattttttcg gtatcaaggc aaaaacaggt agttcaggtg aataaatgac ttcatgttcg
    10861 tgccagggtt tgaccattag cgaatgatcg aaccgttttt tcagttcttc gatgatttga
    10921 tcatgtgtcc agtttcttgc aggaaaatgt tggattttct ctgcatcgat caaaaagaca
    10981 aaatcggaat gaaaagtctg ggtaacttct tccgagagag aattcaagcg ttcgattgga
    11041 tccataaaat cacctttcga cttaaaattg ggtagtatct gggtcttttg ctttgccgac
    11101 tacaccatct aactgatcga tttgtttcat ttcttcttct gttaattcaa aatcaaataa
    11161 ttcagtattt tctttgattc tgcttggtgt cactgactta ggaagaggaa ggaattcgtg
    11221 ttgcagcgac caacgcaaag caacttgtgc aatcgtcttg tcatgtgctt cagcgatttg
    11281 tttcatttca ggcacatcga agattttacc agtgcctaat ggactatatg cttcgaggat
    11341 catatcatgt ttttttgcat actcaactac agttgtctgt aattcgcctg gtgcaaggaa
    11401 aacttgattg accatcggca taatttttgc tgtttttgct agtgtatcta gatggtgggg
    11461 taagaaattg ctcacaccga ttgctttgat tttacctgct tcatacagtt cttcaaatgc
    11521 tcgccatgta tctgcatttg cttgttccca attatcacga aaagcaacag ggtttggcca
    11581 gtggatcaaa aacagatcta aataatctgt ttggagcttc ttcaatgatt cttcaaaaga
    11641 agacatcaca agttcatagc tgtggtttgc attccataat tttgttgtca agaatatctc
    11701 ttctctagga attccacttt ccttaatcgc ttggccgacg ctttcttcat tcttgtatcc
    11761 ttgggcagta tcgatatggc gataacctgc agccagcgct tctttaacag aggaaacagc
    11821 gacatcccca tcaggtgttt gccatgtgcc gaatcctact accgggattt cgtaaccatt
    11881 tgatagacga taagtgcttg ttaatgaatt catactttca gctccttttt tattcgttca
    11941 actataagtg tagcgcattt ttcgaaaaaa agaatccatt ttgtttctac tcttgaaatt
    12001 tttgtttaaa agaaaatgat tttgattctg gtatttttga ttttttttcc gtaaactaag
    12061 acagaaggga atgagaaaat ggatatctat ttaaaaaaag caattctgca tattattgac
    12121 agagaaactg gttctccagt cttttcacaa aaagagctgg atctgacaaa agaatatatt
    12181 cgtgattttc ttcaaaagaa gatccaaaaa atctcttctg ctcaaacaaa aactggacaa
    12241 ctagcagaag aaagcatgat cgttgaatca cttaaaatcg ctcaagaaaa ttttgtggaa
    12301 gcaagtgaag caatcgttca gcggtggttc gatatctacc aagaaagtga agaagcacct
    12361 agtgcagatg tatttgttgt cttgtatgaa ttagatacag tgatgtactt atctttattg
    12421 aaagtcaatt atcgtgaggc ttacactcac tttgttgaag cagacgaagc agggatcgac
    12481 aaccgcttga ttttgaatcg agcaattcta ggggcgaaaa cacaaaaagc agatgaagca
    12541 ttcgctgtga atttgactga ttggacctac gaattaattg aaaaaaaata tgagttttct
    12601 ggcaaaaaag agctttattt ttctggaaga gtgatcgaaa gtgttcctgc accttcttta
    12661 gaagaaaatg taaaagtgat ccaaaaagta gccaagcatc ttggaaaaaa atttgagaca
    12721 gaagaatttg atatcatggc tgatttgaaa gaagctgtct atgatacgat agaagaaaaa
    12781 ggtcggttgg atcatgagat gattgcggaa aaagtcttca aggaaaacat cacggcaaaa
    12841 atggctttta aagaagaagt acaagagcaa ggatttgtcc cagaagcacc tcctgtaaaa
    12901 gaagtacaag aaatttctga gaaaaaattt ggaaaacaaa aattcaagct atcaaacggg
    12961 atcgaactga tcgtaccagt agatgtatat cgcaacccag atttgattga atttgtgaat
    13021 aatccggatg gtacgatatc cgtgatgatc aaaaatgtgg atgaagtatt gaatcgttta
    13081 taaaaatgac tagaatatta ggtgttcaag taccagtgat cagttaaatg aattgtgtca
    13141 agagaaggat cggaaaagac gaatataaca aaaaaagagc gtgagacaaa agtaaagaac
    13201 acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcttat
    13261 ttcttggagt taaacgcttc tgtcccagcc tctttttatc taataaaatt ttaagaccat
    13321 ttactaatta tcgatttttt ctgccgcatt tttaggcact tcagaagcct ttatttcttc
    13381 ccagcttaat acacctttac gaggattgac tttgagttta agatacgcat tcatctttaa
    13441 tggatgttct ctttcttcgc gaagcgtcac ctgttttttt tcaccatctt tattataagc
    13501 cgcttgcttg taggtataag tcgtgaattt ctctccgtta tctgcttggg tagctgacat
    13561 ttcgccattc gtcgtgattt ttgtataata agcttcgccg ccgtaaaaat aatgataagc
    13621 tgcaaaactt ccgccgagaa atatgcctaa aacgacaagt acgccaatta attttttcat
    13681 gtacttcacc tctttcctta ttattaacga aatagaagat ttcgtctatt gaaagttgtt
    13741 ggcaacagct gaattttaaa cttttcctca ttttgttctt attgcttcaa aagaaagtta
    13801 aggactatct tgaagttttt ctcaaaaaac acattttttt atttataaaa ggtacttttt
    13861 tcgcgtataa gacagacgag tgctcttagt tgactttgac caatattgac gtataataag
    13921 cttagttcga atcttacaaa aagtaagtcg gacgtattct ttttgattta gtcaaaaaaa
    13981 gactataata aaaaggtgag agctgaaatt tttcagtgat ctatattatt taggaggttt
    14041 gtaaattatg gcaaaagaac attatgacag aagtaaacca cacgttaaca ttggtacgat
    14101 tggacacgtt gaccatggga aaactacttt aactgctgcg atcactacag tattaggcaa
    14161 aaaaggccta gcaaatcctc aagactatgc tagtattgat gcagctccag aagaacgtga
    14221 acgtgggatt acgatcaata ccgcacacgt agaatatgaa acagaaaaac gccactacgc
    14281 gcatattgat gcaccaggac acgcggacta tgtgaaaaac atgattacag gggcagcaca
    14341 aatggatggt gcaatcttag ttgtttctgc aactgacggt ccgatgcctc aaacacgtga
    14401 acacatttta ttgtcacgcc aagttggtgt aaaatacctg attgttttct tgaacaaagt
    14461 tgatttagtc gatgatgaag aattgatcga tttggtagaa atggaagttc gcgagttatt
    14521 gagcgaatat ggtttcccag gcgatgacac tcctgtgatc aaaggttccg cattaaaagc
    14581 attgcaaggc gatccagatg ctgaagctgc tattatggaa ttgatggata cagtagatga
    14641 atatatccca acaccagaac gtgatacaga taaaccatta ctattgccag tggaagacgt
    14701 cttctcaatt acaggtcgag gaactgttgc ctcaggtcgt attgatcgtg gtgctgttcg
    14761 tgtcggtgat gaggtagaga tcgtagggat caaacctgaa acacaaaaag cagttgtaac
    14821 aggtgtagaa atgttccgta aaacgttaga ttacggggaa gctggggata acgtaggcgt
    14881 gttgttacgg gggatccaac gtgacgatat cgaacgtgga caagtacttg ctaaaccagg
    14941 ttccattact ccacatacaa aattcaaagc agaagtgtac gtgttgacaa aagaagaagg
    15001 tggacgtcat actccattct tcaacaacta tcgtccacag ttctacttcc gcacaactga
    15061 tgttacagga acaatcacat tgccagaaga tacagaaatg gtcatgcctg gtgacaacgt
    15121 gacgattgat gttgacttga tccatccaat cgctgttgaa aacggaacga cattctcaat
    15181 tcgtgaaggc ggacggacag taggttcagg tatcgttaca gagatcgaag cataagaaac
    15241 gcgactcgaa tattttggta atgaaaaatg tttaaaataa aagagcggtc caaacaggat
    15301 ttctgtttgg accgctcttt gttcttcaat tgtataagaa gagattgtag aagcatgaac
    15361 tttcgtttta cttcagtctc ttttattcat catgagagtg ttcatctatc ggtatgcagt
    15421 ttttaatact ttcttcgtat ccattttttc aaaccactta ttctaccttg tgtctgctca
    15481 actggcgcat tatcaataat cagcaaagca atcataacat atgcaatcca actatctcta
    15541 gaatatagag tataacgtga gacccagttt gtagcgtact tgcttttata ttctcttaat
    15601 ccatggaaag aataaaaatg agaaccaaac tcgtaaacca agtatgcaat cctttcctgt
    15661 aaaaaactct ttctggaagt tccgacattt gaaagagggg ccatccctaa attgaaccat
    15721 tggatatgtt cttctttcat ataggcaaac aaatgcaaga acaagaaatc catactgcca
    15781 gaaggtgctt ttttagaatc atatcgcatc agatcaatcg ttcctacttt attgttcgtg
    15841 taagtaggca tgatcgttgc aaaagcgaca atttcttctt gggtattttt gacaactgca
    15901 ataggcgctc tttgtagata atcttcagag aaaaagccta aagaaaaccc tttttccttt
    15961 cgtgaaccca accaattatc agagatattt ttgaaagtac tcatttgctc agtagaaaat
    16021 ggtggttgta agacatcaaa tgtaaatcct tctcgttcga tccgattcaa gacagcacga
    16081 gtgcccttca tttttttccc tgaagttgta aaactattca ggtctacata agcttcttcc
    16141 cccatcttaa tgaaatcgta gccgaattca tgcaaaatca taacaatctc ttcgcttgtt
    16201 tcgtagaaaa ctggtaaata gcatagtcga tctgtttctt cgataaaagc ttcgattgct
    16261 tctggaaaat cttctttctt accagaagga tcacccatta caatacattt attattgaag
    16321 caagcgaatt ggagaagaac agttggttca ccgtcttttt cataggcaaa catccgctta
    16381 tcttttaaga agatcaattg actatcggag tttcctccat aagttgtaag gatcttcagg
    16441 gccttttctt cattaaaggc ttcgccaatc tgtttctttt ctccttgaag gaaatgaaca
    16501 aatagaacga tcatgaaact aacagcgatg atggccaaca atcccgaaaa ccaaatcttt
    16561 tcagaaggaa ataagaaaaa ggaaataaaa tggtgacgcc gatgagggaa atctggcaga
    16621 ttgtataccc caataacgat gtaaagcaag gacagtgcac caataatgat tccatcgatc
    16681 gtacgccact cccatgaata gatcaattgt tcccgaaata attcgttttt agaagcaaga
    16741 atgatcagaa gcagaataga caagaaaacc accggtgtga aactaaaatc acttaataaa
    16801 acataaagta aagctagaac aatcaagaag attgtaggca aatatgctcg tttcacacgt
    16861 gctgcgatcc ctctgcccat aatgattaaa agaaatccca atagtatact aggaaattgg
    16921 ataataaaat gaaatttcaa tgggttcaaa ctatgaagcc agcgaaactc tgtaaaagct
    16981 tgcgggatag tggcagaaag tactagcatg attccagaaa agtataataa gacaaccacg
    17041 attttatgcg caatttctgt tgctaattgt ttagggatac cagaataacg ctgatcaaaa
    17101 gtggaaccga tatttttgaa gaaaaagacg attccaatca aaaacggaat gatgtagtag
    17161 aacaggcgat agagcaagat ccacacaacg actatttctc ttggcacacc aatagcggac
    17221 aacccgataa tcatcattac atcgaaactc ccaatttctc ctggaatcat agacacgatt
    17281 cctatcactg aagcagcaat gaataatgga attgtttgcc acagatcaat ccggatttcc
    17341 attaaataac caacagaaag gaaactaaac aggacaccgg tccattccaa caatgaaact
    17401 agcaatagct gtccttgagt tttaagtgag gtaccaccaa ggaatccttt ctttttgaaa
    17461 gtagtaaata aaaagacgac aggaaaatat agaccgccgc cgatcaacca tatccaatat
    17521 tgcttcaaaa aaggagcaac tggcgtgaag actacaagta aaaaagaaag cagactgtaa
    17581 atagaaagtc cagccatcaa aaacaagaaa actttcgata atgcttggat cacgtttctg
    17641 ctctctttct tttgtccata tagttcagag cgaagcccca cactaacaaa cccgccgaaa
    17701 ccggcaatat tgttgatcgt attgatcagc cagcttgtct caaacaggta acgaggattc
    17761 tgcttttgtt caagcatccg attcaaaata atatcataac caaccatagg taacacagag
    17821 ataagaccaa tcaatagcat tagtccgctt ttccatacag gtatagtggc aaacgtttct
    17881 cctaattgct ggatagatag agttttaccg atggacatca attcaccgac aataatgaca
    17941 atgacagaaa tcaaaaagat tgttttgaac agtcctaggt ggtttttcag ccattgtatg
    18001 gtatttttta acaaataaat caccttctat tcctaatagt tatgtttcag aaagaaattg
    18061 aagaacttct cgattgaagc gttcaggatc ttttctagct aattcatgtc cttgttcttt
    18121 cacaagcaca aaggaagctt ttggtatagt tttagctata tacaaagaat gttttagctt
    18181 gattacatct ttttttccca cgatgatcaa agtgggggag ttgatttttt ttaaatcatt
    18241 ttctgtaagc ccgatgtcat gcagcaaaag tttgataact aaaagatttt tgcgaagact
    18301 tgggcgaaat aaagagagta accataccca tgcataatga atattactga tgactctggc
    18361 agaaaaacga acgcctttaa caagcgtgtt tccagaattt aaaatcaatc gatgaacttt
    18421 tttagggaag ctgctggcaa ataccagtgc tagatttgct ccatcactaa atccaagaaa
    18481 atctgcctga tcgatttttt ccagcaacat gatcgtattc agatcctcgg ccattaactg
    18541 aaaattcaac atagatgctt cgtttgtcga acgaccatgt cctcggctat ctactagata
    18601 cacggtatag tagcgttcga gtacagggac ctgttctgaa aaaaagcggc cgctgccgtc
    18661 attgccatgc agtaaaaaga gagggaagcc ttgaccgctc ttttcatagt aaatttcgct
    18721 cccatctggc atttttgcat atttcttttc ttttttcaca taatcaccta ctgtgtaaag
    18781 tataacgatt atatcagtca ttgggcaatt gtttttactg aaaaacaacg atcattttcg
    18841 taagatgtca aaaaatacag gaaaattcat tttttcttaa ggcgtacaca gaaatcaagg
    18901 aagaaatagc caagaagata aagaaatagt aagataaaaa aagatgtcaa aataactagg
    18961 tgatgaaatg aagcgaaaac gattatctat tatccgtatt cttttttaca tgatcattct
    19021 gacggttatt ggtgcggtgt tgtttttagg aggaagaact tatcgttcgg taaaacgagt
    19081 ggaaagctat caaatagaaa taacaacagc tgcaaaaaat tatcatatgg aaaactacga
    19141 gaatttgatc aaggcgataa tattgaccga atcaaaagga aaagggacag atctcatgca
    19201 gagctcggaa agtctgcatg gagaagctga gatcattgat aatccagctg acagtattga
    19261 tcaaggtgtc aaatatttgg cggaaatgat agtagaagca aagaaacaag gatgcgattt
    19321 atggacagct gtccaagcat ataattttgg gaaagattat attgcctatg taaaaaatca
    19381 tggcggagaa aacacagtag aattagcaga agcgtattct aaagatgtgc tgagcccact
    19441 tttaggaaat gaggaaggga aacaataccg atattggcgg atccagtcct tgctttacaa
    19501 tggcggctat ctctatcata atggaggtaa catgttttat cctgatattg tcaaaatgaa
    19561 tgaaaaaata atagagacct atgaaaaagt ctttaagtaa aatagcagga aatgttcaat
    19621 catgaataaa attaaatgaa agaaggagtg cgcaaatagt ggaaaaaagg caaaaaatgg
    19681 aaaatcaaga agtggggtac acaaccaaag actggatgtt acggtttgtg aagggaatgt
    19741 ttatcggttc aggatttatc ttgccaggag tcagcggtgg tgcattagca gctgtctttg
    19801 gcatgtatga gcggctgatt tcttttttag cccaccttac aaaaaatttc aaagaaaatg
    19861 ttttgttttt tctgcctgtt ggtctaggcg gagtgacagg catctttcta ctttcttttt
    19921 tagtcagttt tttattaggt gcgtatgaaa caacgatatt atggtttttt gtcggatgta
    19981 tcgctggaac tgttcctgcg ttatggaaag aagcagggaa aaaaggaaga gatacttctg
    20041 atgtgatcat catggttgtt acttttgttc tggcttattt gtttttatta tacggcgctc
    20101 aattatttga cgggcaagtc gatcagaatt ttggaacatg gctgattgcc ggcggattga
    20161 ttggtttggg tatgatcgtc ccaggattga gcccttctaa ttttttggtg tatatgggat
    20221 tgtataaagc gatggctgac ggatttaaag aagtacgact tgaagtgatt gtgccgatcg
    20281 gaattggtgg gattctttgt gttcttggat tgtcaaaaat catggattac attttctcaa
    20341 aggcatatag caagctattc catttcattt taggtgtagt ttttgcttca acgattatga
    20401 tcattcctac cgattacgcg ggattgggag ttgctggggt actagcttgt cctgttcttt
    20461 ttatagccgg tacagcacta ggttggtgga tgagccgttt ggaggaacaa tataagtaaa
    20521 gatagattga taataaaaac agcacacggg tgattcaaac aaaaaggttg gcatcttttt
    20581 tgtaaaatca ttcgtgtgtt tttctataaa ttttataaaa acaagttgtt tttaatcttg
    20641 gaacacgcta gacttaaaca cgtgattatt tttgaaagtt ggcggaaaat ggagaaaaag
    20701 aaaatgaatg gacttacaca gcaagaggta gttcagaaaa tggcttctgg ccagcaaaat
    20761 gactaccagg aagatgcatc aaaaagtaca aaacaaatat tcagcgataa tatattgacg
    20821 ttattcaatt ttctgaactt tggtattggg atttgtctgt tatttgtagg tgcgtattcc
    20881 aacatggcgt atttagcaat tattgtcgtc aatatcgtta ttggaattta tcaagaaata
    20941 catgcaagaa atcttgtacg gaaattatcg attgtttccc aatcagaagt actagtgatc
    21001 cgggacggta aagaacaatc gatttctact aaagagctgg tattaggaga tttggtaaaa
    21061 atcggagcag gagaacagat accagccgat ttacaggtta tatctggacg ggcagaagca
    21121 aatgaagctc ttttgacagg cgaatctgat ttgattgtca aagaaactgg agctgaattg
    21181 ctttctggaa gctttttgac aagcggttct gtcatggctg aagttatccg agtaggtgca
    21241 gataattatg ctgtccgctt gactagcgag gcaaagactc acaaagctat tcgttctgaa
    21301 cttgtagatt cgattcgcaa agtatccaaa ttcactagtt tcgtgattat tcctttgggg
    21361 atcatactat ttttggaagc actgttcatc cgcgatgctt ctatcaaaat ttctgttatt
    21421 gcatctgcag cagctttgtt aggcatgctt ccaaagggtt tggtattgct aataagtatt
    21481 gcattaacga caggggtaac caaactggca aaaaaacgaa tccttgttca agacatgtat
    21541 tcaattgaaa cgctggccca tgtcgataca ttatgcttgg ataaaacagg gaccattact
    21601 gaagggaaaa tgagtatcca gcgggtagaa gcactacatc cgctgtacga gaaagccatc
    21661 ccagaaatta tggggacgta tctggcagaa agcagtgata acaacatcac tatgcgggct
    21721 atgagagatt attttgagct ttctaatcgg tatgatgtga caaacaaagt cgcattttct
    21781 tctgatcgca aatggggcgc gatggaattt ccagaactgg gagtcgtgta cgtaggagcg
    21841 cctgaacgtt tggtggaaga agatcggctt ccggacagtc taaaaacagc ccaagcgaat
    21901 ggctatcgcg tacttatgtt ggggatcgct ccaaatcagc aattgaacga agaaaaacca
    21961 gcatttgttg agccggttgc tctgtttgaa atcgatgatc caatccgaaa aaacgctcaa
    22021 gaaacactag cttatcttca tgaggaaggt gtggatctga aagtgatttc tggagacaat
    22081 ccttttactg tctccaatat cgctagacgt gcaggattac cgaattatga tgcgtatgtc
    22141 gatctttctc aaattactga agaaatggaa gtgcgagaag ctgctcatcg ctatacggtt
    22201 tttggacgag tgagtccgca gcaaaagaaa ttgctggtaa atgaattgaa agaaagcggc
    22261 agaacggttg caatgacagg cgacggtgtg aatgatgttt tggcgttaag agaagcagat
    22321 tgcagcattg ctatggcaga aggtgatgga gcaacacgtc agattgcgaa tctggtattg
    22381 ttggactctg attttactac attgccagaa gtcctgtttg aaggacgccg agtggtcaac
    22441 aatgtgacga aagtctcagg catctttttt atcaaaacga tttattcatt tattttatcg
    22501 attatatgtg ccgttacagc aattggattt ccgtttatcc ctatccaaat cacgctgatt
    22561 gatttagcga ttgaagggta tccttccttc tttttatctt ttgaacaaga taaaagaaaa
    22621 atcacttacc gttatttgcc tacagcactt attaatgctt taccaaatgc cttgcttgta
    22681 gtagtgaata tcattgcggt gtatctactt ggaaaaacat tcgcatgggg aaatgctgaa
    22741 actactactt tgatgtatta cttgctgatt gggatcagtt gcatggcagt tgtaaaagct
    22801 tgttatccct tcaacccatt gcgcatcttc ctagcagtaa cgacgatcgt agggatatat
    22861 gtagctgcaa tgctgttcca tggaattttg gaagtaaact tagggttggg agagagcgta
    22921 ggatacttct ttgtattcat gctcgtaaat attgttcttc gagtattgta ctggaaggct
    22981 gatattcaaa atcagttact gaaaagagta gcaaattgat gcttaatgag atagaaatgt
    23041 gttatacttg aaaaagcaat gaaatatgat acaatcaaac atgtataatt gtgtccttta
    23101 aaatcgcact gatttcagtt agtgcgattt tttaccggtt attctcctga aatatcttct
    23161 gttaaaccca aaagatatca atagagcagg gggtgaaaaa atggctcaac ggattcctca
    23221 agaggttatc gaaacagtac gcagtcaaac gaatatcgtt gaggtgattg gacaatatgt
    23281 ccagttgaaa aagtctggta aaaattactt ggggctgtgt ccttttcatg aagaacgtac
    23341 accgtctttt tctgttgcag aagacaagca gatttttcat tgctttggtt gtggtaaagg
    23401 cggaaacgtg tttacttttc ttcaagaact agaaggtcta agttttccag aagcagtggt
    23461 aaaagtggca gacttcgaac agataccgtt agaagatcaa tggcgtcagc gtactatagc
    23521 tgtccaaaat cctgaatctg ctacggggaa actgattgct gctcatgaaa aagcagcgga
    23581 aatctatcat cacatgctat tgcatacaaa agctggacag ccagctttag aatatttgca
    23641 aagtcgtggg ttgtctttag aattaattga agaattcaac ataggttttg cacctaatga
    23701 gcgttctttt cttcagcaag tattcaagaa tgaatcaatt tcaaatgaat tgttagaacg
    23761 ctctggattg tttgtcacgc atgatgatgg ccgtatgtct gatcgttttt atcaaagagt
    23821 gatgtttcca cttcgtaatt ctcaaggcca gacgatcggt ttttcaggac gctggcttgc
    23881 acctgaaaaa gggcaggaaa aagatcagcc caaatacttg aatagtccag aaacagaact
    23941 gttcaataaa agacaagttt tatttaatct tgataaagca agaagcgata tccggaaaaa
    24001 cggagaaatt ttgttgttcg aagggtttat ggatgtgatt gctgcatggc aggctggtat
    24061 caaaactgga ttagcttcta tggggacaag tctaacagct caacagatcc aacaaatgga
    24121 aaagctgacg aaagaattgg tcttttgcta tgacggagac aacgcaggtt ttgaagcaac
    24181 caatcgaggg attgaattgc ttcggcaaaa cagtcgatta gcactttctg tcgtagtcgt
    24241 tccagaaaag cttgatcctg atgaatatct ccgtaagtat ggagaagcat ctttccaaga
    24301 attagtggct catgggcgcg aaacagtctt tacttttaaa tcacgttatt taaaacaagg
    24361 gaaaaattta gataatgaaa aagacaagat cgaatacctc gaagaattac tagttgaact
    24421 tagccgagtt gtttcaccaa tcgaacaaga tatgtatcta tctcaactgt caacagagtt
    24481 tcagatcggc agagagacga tacaaaaaca actgcgcgat cttcgaagga caaatcagca
    24541 aaatcgtcaa gaacctcagg aaagtcaaca cactcaggtt acgagacaag aaactgcacg
    24601 gaaaaagaga cctttgacgc aagtagaaaa ggcagagcag attctattat atcgtgcatt
    24661 taaagaagtt tctgtccgta atattttaaa agaacgagaa tttcagttta ttcatgatgc
    24721 atatgcagaa gtttattttt tattcgatgc atatgtctca caacattatg aattcaacct
    24781 tgcagagttt ttagactttt tgcaagatga aaatttaagg cggttggtgg tggaaatcga
    24841 atatttaccg gtggcagaag aaagttcgcc acgagaaatc caagacttgc tagatgtgat
    24901 cagcaaatct ggcttagctg atgaaataac attaaaaaaa caaatgcagc aagaagccag
    24961 acgaactgga aacaaacagc ttgagcttga attgacgatt gaaatcatca atttaacgaa
    25021 acagttaaaa caagccaaat agctactcaa ggggggcctt cttttatggc aaatggaaca
    25081 gataaaaaaa cgtatgaagc agcagtagca gctttcatca aagaaaacaa acccaaacga
    25141 caagttgtat atgatgattt atccaatcag cttgctacac cattcacttt gaatgcagat
    25201 gaaatggaca aattgatcca aaagattgaa gacgcaggta tcagtgtggt ggatgaaaat
    25261 ggtgatccat caattcacag cttgaagagt gctgagaaaa aagctgaaca agcaaaaaca
    25321 gaagatcttt ctgcgccaac cggtgtaaaa atcaatgacc cggttcgtat gtatctgaaa
    25381 gaaattggcc gtgtctccct tttaactgcg gaagaagaag tagcgttagc attgaaaatc
    25441 gaagaaggcg accaagaagc gaaacagcgt ctggcagaag caaaccttcg tttagtagta
    25501 agtatcgcta aacgttatgt aggacgaggc atgcagttcc ttgacttgat ccaagaagga
    25561 aacatgggac tgatgaaggc agttgaaaaa tttgactacc gcaaaggatt taagttttca
    25621 acctatgcta catggtggat tcgtcaagcc atcactcgag caatcgctga ccaagcacgt
    25681 acgatccgta ttccagttca tatggttgaa acaattaata aattgatacg tatccaacgt
    25741 caattgctgc aagaccttgg aagagaacct acgccagaag aaatcggtgc ggaaatggat
    25801 cttccaacag aaaaagtccg agaaatcttg aaaatcgcgc aagaacctgt ttcccttgaa
    25861 acgccaatcg gtgaagaaga tgactctcac ttaggtgatt tcatagaaga ccaggaagca
    25921 acaagtccag cagaacacgc agcttatgag ttactaaaag aacaattgga agatgtacta
    25981 gatacattga ctgatcggga agagaatgtc cttcgcttac gttttggttt agatgatggc
    26041 cggacgagaa cattggaaga agtagggaaa gtatttggtg ttactcggga acgtattcgt
    26101 cagatcgagg ctaaagcatt aagaaaattg cgccatcctt ctcgttcaaa acaattaaaa
    26161 gatttcttag aataagcgtg gactattgac cagaagagaa atcagtaaaa ctgtttggtt
    26221 tattttaaaa gaaaaaaagc ttgagactac gcagtctcaa gctttttttt ctataggaag
    26281 gaaattcatt cattattttt aagtaaatcc taaaaataag ctattggcgt gacgtataga
    26341 atatgataga ttaaatggta gcgtaaacct agtaaaggag ccgatcgcta agtgatgaaa
    26401 aaatgtccaa attgcggatc gaaaaaaatg gagcagaata ctatctgtcc agactgcggt
    26461 catcaaataa ctgcagaaga tcagcaaata aaagaatcaa ttgaagaaac aaatacagat
    26521 gcagctatgc aggataaatc aactgaagat gcagtctttc ctgatacaga attaaacgat
    26581 ccaatcgaat ggtcggaatt gaaagattta cctctagaat ctgtaatgga attattcgaa
    26641 aaagcagaac cagaagacga agaaccacct gctcacgatc aagtaactaa tatagagaaa
    26701 aatgaacaga agaaagcaac tgttcatgag acttcggaag aatcaggaaa gaatcaagaa
    26761 gaaacgcttg aatcaaaaca aaaaagaaga gtagctgaac tgaaagaaat cgtcgataat
    26821 gaggaagaaa attcgatact atccgcttat atcaaagcac atcgtgaaga tacaaaagaa
    26881 gagcacgcga aagagctgct caaaatgatc agtgaaaaaa ttgcacagga aaacaaagta
    26941 gagccagaaa aggaacaaga aactgaaaaa gactctgata gagaaaacgc cacaggtaaa
    27001 gaaacagtaa aaataaaaga cgtaaaaacg gatatcacag aaaaaactga agtatttact
    27061 gaaaaacaat tagaaaagga aaaaacgaat aaactgccgg aagcagagaa aaaagaaata
    27121 ctcgaaaaca cagacaaaaa gtcaacagag cccgatgaaa taaatagtga atccgatgct
    27181 gaaaatcaat ctgtaagcaa agagggtact gatcttgaag caaaaataga aaaagaagag
    27241 aaccagccta agctgtttga agcagaagcg aaatcaataa caatcgtgcc agatactgaa
    27301 aaaacacatc aagaatcaaa cttagaaaaa acagatccag ataaagcgca aaagaaaaaa
    27361 tcaaaaaaag taccttatct cgttttagca gcagtacttc tactcggtgg aggaagctgg
    27421 gcttattatg accatcatca aaaagtagaa gccgaagtag cagcagaagc aaaaagacag
    27481 caacaaatgg aagaacttca tgagaaactt gctgcctttt atacagatga tcaccatcaa
    27541 tttattcgtg cagatatgat tcatcaagat ttatcaaaac taaaaaatga tttatccagt
    27601 cttaagaatg aaaaaggata ttcagagtta gaaacaactt accaagatat ccaatctaag
    27661 atccgagata tccaaaaagt gaatgagtgg ttcatagcac ctgccatcgt cgatgatcat
    27721 ctagcagatg acatcaagtt gaaagcagat aaagagatcc aagcaatcga aacggataac
    27781 acagattttg gaaaattgat tcaacaagca caaacagaag ctaaaaatca atatgctgaa
    27841 ttgcaaacag ctaaagaaaa aacagctata gtctttaaag acggaaaagt agtggattca
    27901 gcaacacgcg accaatacaa gacggcaaaa gaagcagtag aaaaagtgaa aaatacagat
    27961 ctatctgctc agttgaatga acaattgaaa caagtagata agatgctcac tgataaagag
    28021 aaaaaagaag aagaggaaaa gaaaaaagca gaagaagcga aaaaagcagc tgaagaacaa
    28081 gcaaaacaag ccgctcaagc agcagctgca gctcaagccg ctcaacaagc tgcggtcgca
    28141 caagcagcac agcaagaagc ggcatcctcg accccttcct cttcaaactc agcgaatcag
    28201 ccaatcaaat ctgccagagc aagtgatatt gccgatgcat ctaatccagc atggacgtgg
    28261 gcaccaggtg tccaaagcaa agtgattgct acatgtatcc aacgtggata tatcgttgaa
    28321 ggaggttaca gattagaaaa agccagaatc gaaaatgggg aaggctatta taatctatat
    28381 gccacttcaa caaaatctgc attgatgaaa ggcattggag aaagtgcttt gccattttac
    28441 atcgtaacca tcaactgtaa aactggatgg ttcggaggaa atgggagcaa ttaattttag
    28501 gcaaatgaaa gaggctgtga gcctgccgtt taacaccgtt tattcgaaaa aggggagtat
    28561 gacacaactg ttgtcatact ccttttttca cctttccaaa ccaaggctca gctattcaag
    28621 tagataacat tatgttatga tagcaaagaa atgtattaag aaaacctttc actatagagg
    28681 gagaaaaaaa tgaacgattt attagcaact atatttacag gtattattat tgatgaaaat
    28741 gaaaaaaact actttgtcca aaaaaacgga caaacctttg gcttaagcaa atcagaaggt
    28801 gaacacaaaa tcggtgaagc agttgaagga ttcggttacc aaaatcaaaa acatgaaaat
    28861 cggatcacta cacaaattcc taaaagccgt atcggtcatt acgcttttgg aatcgttact
    28921 gctacacgaa aagatttagg tgcgtttgtc gatatcggtc ttccagataa agactttgtc
    28981 gtatctcttg atgaattgcc gacaatgaca gagctatggc ctaaaaaagg ggatcgcttg
    29041 atgatttccc ttagagttga tgcaaaagac cgtatttggg gcagtttagc agatgaaaaa
    29101 atattcaaat ctttaagcaa atatggaaca gaagaaatga aaaacaaaga tgtgatagga
    29161 acagcatacc gcttgaaatt aacaggtacg tacgttttaa cggaagattt ctatcttggg
    29221 tttatccatc cttctgaacg atataatgaa cctcgtctag gcgagcagat aaaaggtcga
    29281 gtgattggtg tccgaccaga cggtgtactg aatctttcat tgaagccaag agcatatgaa
    29341 gctatatcag atgatgcaca aatgatttta accattttag aacgttctgc agatcagcaa
    29401 atcaacttta cagataaatc ggatccagaa gaaatcatga aagcttttgg tatcagcaaa
    29461 ggagcattta agcgcgcatt aggtaatctt ttgaaacaag gattgattac acaagaaaac
    29521 ggtgtgacaa aactagtcaa aaaatctaat tgagaatccc ttgcatgttt ttcttaacta
    29581 gcttataata attttaatta acatagtgtt atatcttttt ttatttgtaa ttattataaa
    29641 ttgttggggt tgagtaaatg gtcatgaatg ctatttcggc gatgaaaaaa acaaaacagc
    29701 agctacatga agcggggttt aagctgacac cgcaaagaga agctactctg cttgtgcttt
    29761 tggaaaatga gaaagagcat ttatctgcag aagaaatctt ctttttagtg aaaaaaaaga
    29821 gtccggaaat tggacttgct actgtttatc gaacccttga aatcttgaca gaattgaaaa
    29881 ttgtcgataa agtaagtttt aatgatggat tagcccgtta tgatttgaga aaagaaggcg
    29941 ccaaacattt ccatcatcat ttactatgct tgaactgcgg cacgatcgag gaagtagaag
    30001 aggatctttt acttgaagtg gagcaagtag tagaagaacg ttatcatttt ttagtaaaag
    30061 atcatcggct aacgttccat ggaatctgtc aggaatgcta tgaaaaagaa aaaagaaacc
    30121 agtccgtctg agtgtctaga cggctttttc tttgctcaga ttatggaaaa agactatcag
    30181 aatgaagcgg tatctgatat gatagagagg catgaggggg attattatga aagagcaaat
    30241 caacgattat ctccactact tgaatattga acggggattg tccgccaata cacgacaaag
    30301 ctatgaacgt gatttggaac agtacttgca ttttctacag gagcagaaaa ttgcttcatg
    30361 gcaggatgtc gatcgctttt tagtcatccg ttttctggag aagttgcatg aagaaaaaaa
    30421 agcgacagca actgttactc ggatgatctc aagtctcaga agattccatc agtttttacg
    30481 gcaagagcgt atcactgatc atgatccgat gcaacatatc gatacgccga agaaagcaca
    30541 gaaattacca agtacattga ctctgacaga agtagaaaag ctgattgaag cacctgatac
    30601 gacaaaaacg ttagggataa gagatcgtgc aatcttggaa gtcatgtatg ctactgggat
    30661 gcgcgtcagt gaattgattg gattgaaatt gggagatctt catctttctc ttggactggt
    30721 acagacagtt ggaaaaggag ataaagagcg gatcattcct ttaggtgatt atgcgatcca
    30781 atggctggaa cgctacttag acgaagcacg tccattactt gtaaaggacg catctgaaat
    30841 gcatgtattt gtcaataatc atgggaaagg tctttctcgg caaggaatct ggaaaaatct
    30901 gaagcagtta gtacgagagg ctgggattta caaagaagta acaccacata cgctgcggca
    30961 tagctttgcc acacatctgc tagaaaatgg agctgatttg cggacagtac aggaattgtt
    31021 aggtcacgcg gatatctcta cgactcagat ttatacacat atcacgaaga aaagaatgac
    31081 ggatgtttat aagcagcatt ttccaagggc ttagtcatag gaaggtgaga agatgttaac
    31141 acattaccgg aaagaaaacc gtaaaattgc tatgggacta ttgagtttcc ataagaaact
    31201 gacaggtaaa acttctttgt tgaaggagat tgatacgtac gaatctgaag acgattatga
    31261 actttttttc tttacgccag aaggttcaac caacattcaa gggattattg gtatttactg
    31321 ggcaacgaac gatcagctga tcatccatga catttctttg aatccgtctt atcgcggtga
    31381 gaatatgggc tttcaaatgt tagatgaatt acaggaaaag tacccggatg tctccatccg
    31441 tgcaactgaa atcacagagc cttacattgg aaaatggcta gcaaagaaaa agaagaaaga
    31501 gtgaatgagt tgacggagat caatattaaa ttagatgtat tcgagggtcc tctcgatctg
    31561 ctgcttcatc tgattcagca aatggaaatc gatatatatg atataccgat cgctacgatc
    31621 acggagcaat atatgaatta tatccataca atgaaaacac tcgagctagc tgttgcagga
    31681 gaatacttag tgatggctgc tacattgatg gcaatcaaaa gcaagacatt gcttcctgtc
    31741 caagaattcg tcacagacga tgaagaatta tgggaagaag atcctcgaga agaattggtc
    31801 actcagcttt tagaataccg taaatataaa tatgcagctg aaagactgac agaaaaagca
    31861 gaggaacgaa gtctttatta cacaaaagaa cctatgaaca tcgatgggtt agcggatgct
    31921 gaaaagccgt tgaagcgagg acaagtcaaa aaaatcgatt tgatccgtgc catgcagaaa
    31981 atcttagaac gtaagaaatt ttctcaaaaa gtggaaaaaa cgatcacgcc cgatgacaca
    32041 acgatcgaag aacgaatggt atttatcgaa gagcgtataa aaagaaatgc gcatggctgt
    32101 actttcgatt ctttttttga gtcaccttct aaaagtgaag ttgtcactac ttttatggct
    32161 ttgctagaat tgatgaaaaa agggcagatc attgcgcaac aaaaagaaaa ctatgaatca
    32221 attatgcttt atccagcagt aggagatacc gaacatgacg aaattaagtg aacttgaagc
    32281 ccttcttttt attgttggcg aggaagggat cagtaatgaa gaattaagcg gactgttaaa
    32341 tctcagcgaa gcagaagtca caagtttagt gaaagaatta aaagaatctt acactcaaaa
    32401 ctcgcactca gctttgcata tttttgaaac ggaaagccat tatgtattaa cgactaaaaa
    32461 agagctggct cccttactta agcgatatgc gcaagggaca tcaaacacat tatctcaagc
    32521 cgccgtcgaa accttagcaa tcatcgctta taaacagcca atcacaagag ctgaaatcga
    32581 gcagattcga ggagttcagg tttcaggagc ggtacagcgt ctagctgccc ataaactgat
    32641 tgaagaaaaa ggtagagtag aaggaccagg tcggccgata ttatatggaa caactggcta
    32701 cttcttagat tattttggtt tgaaatcatt agaagaactg cctgatacac aagaaatgga
    32761 aatttcactt gaagaagacg tgtcaccaga tctattcttt gatggaatga ataaaggaga
    32821 ataattgtaa tggagagatt gcagaaagta attgcccacg ctggcatcac ttctcgtcga
    32881 aaagcagaag aattcatcct tgcaggacgt gtaaaagtaa acggacaagt tgtaagggaa
    32941 ttagggataa aagttgggaa acatgacact gtggaagtgg atgaagtccc aatttatcaa
    33001 gaagaatacg gttactattt attgtacaaa cctaaaggag ttatttctgc tgttgcagat
    33061 gacaaaggaa gaaaagttgt tacggatctt ttgccgatgg taaaagaacg tatctatcct
    33121 gtaggtcgac tggattacga tacttcgggg atcttgctac tgacaaatga tggagatttt
    33181 gctcagcgcc tgacacatcc aaaacatgaa gtggataaag tttacgttgc aaaggtaaaa
    33241 gggatcgcta caaaatcgat gcttgcacct ttgacaaaag gaataaagat cgaaggacga
    33301 aaaacatctc cagctaaatt ccaaatttta tcagtggatc ctaagacaaa ccacagtatc
    33361 gtggaattga cgatccacga aggacgtaat catcaagtga aaaatatgct tcaagcagtt
    33421 ggtcttcctg tgcaaaaact aaaacgtgaa aaatacggag atctgacttt acaaggccta
    33481 cgtcctgggg attaccgtga actgaacaaa aaagaagtca gcagtttgct gaaccactca
    33541 aaatgaacac ttacttttta taagtgttag atgttgcgtt ttttgaatag atatgtataa
    33601 tgaatatgta aacaaaaatt atataaggtt cgtcttcagg ggcagggtga aattcccgac
    33661 cggtggttat agtccacgac ctattttgca aaaaaatagc tgaatcggtg aaattccgat
    33721 accgacagta tagtctggat aaagaagata gagcttattt gacgacgctt gatcaaataa
    33781 ctctaactct attttccctg gaaaatagag ttttttcatt tggagagaaa cgtcaaaaag
    33841 ttcgctgaag aggagtccta acaggaggat ttcaaatgaa gaacacccgt gtacagaaaa
    33901 tggttgcagt agcgatgttt gctgcgatcg cattagtttt acagtatttt gcttttccga
    33961 tcatgccggc ttttagtttt ttgaagatcg attttagtga tatctcggta ttgatcagca
    34021 tgttcttatt cggtccagcg gccggagttg caacagcgtt cttgcgttca gtattacatc
    34081 tgctgactac aggtttttct ccggataatt tggtaggaga cactgctagt ttcttatcaa
    34141 caacgatctt tacattcccg atttattatt tctttagtgt aaagaaacag cataaaggaa
    34201 gaaacaaatt tttaggtgtc attacaggaa ccatcgctct aactattttt atgagtatcg
    34261 caaattattt cgttattacg cctctttact taatgttttt agggctgaac gcaacacaga
    34321 tgctgggcat gtctttagca aactatgtac tgattgggat cgttcctttc aacttgatca
    34381 aaggattcat cgtgagtgct gtctttttag tgctgcatgc gcaacttctt ccatggttaa
    34441 gtcgaaaaca acatcagttc gaacaacgtc atacagcagt caaataaata tcaaaaagat
    34501 caagaaattt cttggtcttt ttttttggaa aaaaatacaa gacatgcaat aatgtaaacg
    34561 ttttatttgt ttgttttgag tatttttttg atttatgcat aaaaatcaat caattctttg
    34621 caaatgtgaa attcttctcc ttttaaaaga cgaaaatgtt tgtttataaa aaaaaggaaa
    34681 cgctacaatg aaaaagaaga aagctttaaa aacctgaaaa aagaggaggg aatagggaaa
    34741 aacgaacttt ttgaacaaaa gttttacaaa atgcgtgtta ttatttttgt tctgatactc
    34801 tttttgataa aataacatta gagccaaaat tgttgtctct aacggaaaag aaaggaagaa
    34861 gaagatggaa caaacaaaaa agaaaaaatt tcaaatgcct tcagcgtata cggtcttatt
    34921 tattattatt gcagtaatcg ctgtacttac ttggtttata ccagctgggc actatagtgt
    34981 agacgatgca ggcaacatcg tcgctggatc atatgaacga gtacaacaaa atcctcaagg
    35041 gctgtgggat attttcatgg cgccaatcaa tgggatgcta ggtgtaccag cgggggagct
    35101 aactaaagaa acacctgctg caatcccaat ctctttcttc attttggttg ttggtggatt
    35161 tttaggtatt atcaacaaaa caggggcact agatgccggg attgcttcag ttgtgaagaa
    35221 attcaaagga aaagaaaaaa tgcttattcc agtattgatg ctgttatttg cattaggcgg
    35281 ctctacttac ggaatggcag aagaaacgat tccattctat gctttactga tcccagtcat
    35341 gatggctgta ggctttgata cagtagtagc tgtagcaatc gtattagtag ggtctcaagt
    35401 aggctgtcta gcttcaacgg taaatccttt tgcaacaggc gtagcttcac aagctgttgg
    35461 gatttcgatg ggtgaaggta ttggtcttcg attcttgatg ttcgttattt tgttagctat
    35521 ttctattgta tatgtgtatc gttatgcatc aaagatcgaa aaagatccaa caaaatcatt
    35581 agtatacaac caacgtaaag aagatatgaa gcatttcgat attgaagcaa tcggtcgtca
    35641 agaagtaatc acgaaaaaac aaaaacatgt taatgtattg ttctttatta cttttggtat
    35701 catgatcatc agtttgattc cttggacaac cttgaatgcg aatttcacga tttttgaatc
    35761 attgacaaac tggttaacaa accttccggt actaggtacg gttctaggta aaagcatgct
    35821 tccattaggt gactggtact tcccagaaat tactatgtta ttcttagtaa tgtccatcgt
    35881 agtagcaatc acttatggca tgaaagagtc tgaatttgtc tctgaatttt tagctggtgc
    35941 ttctgatttg atcggtgtag caatcgtagt agctgttgct cgtggtatcc aagtggttat
    36001 gaacaatgga ttaatcacag acaccatctt acattggggc gaacaaggat taagttcatt
    36061 atcttcaagt gtattcatca ttattacatt tattttctat atccctatgt cattcttgat
    36121 tccttcaact tccggcttag ctgctgcaac aatgggaatc atgggaccta tgggtgaatt
    36181 tgccggcgtc ggtaaggatt tagtaatcac tgcttatcaa tctgcatctg gtttagtaaa
    36241 cttaattaca ccaacatctg ctatcgtaat gggtgctgta gcaattgctc gtttcgatgt
    36301 ttcagtgtgg tggaaattta ccggtaaatt aattgcgata ttatttgtag ccatctgtgt
    36361 gatcctaggt gtagcagcaa tgttctaaac ctagacaaat acaggtacaa ggtctatcat
    36421 aagaatgaag aaaccgataa acggctatcg ataagcacgt ggataagtca atagctccat
    36481 tcagtgagtg ttttttcttt catcatttgt taagaacatt ctgcttgctg atggagcagg
    36541 cttaaaacgt cattatttga gaagaagagg ttgtgtcgga ggaatttgac atgacccttt
    36601 ttaacatatt aggaggataa tatcatgaaa aaatttgtga cagaacaaca tcatgaacaa
    36661 gcattagaat cattgagcga attgatccga attccttccg tactagatga agcagattca
    36721 ggacaaggac atcccttcgg taaaaaagtg attgaagctt tggataaagt attggaaatc
    36781 agtgaaaagc taggatttag aacatttaaa gatccagaag gctattacgg ttattcagag
    36841 attggctctg gagatgaatt attcggtatc ttgtgtcata tggacgttgt tccagcaggg
    36901 gacgaaaata attgggaaac aaaaccattc gatccaacta taaaagacgg ctggttagtt
    36961 ggtcgcggat cccaagatga caaaggtcct tctattgcgg caatgtatgc cgtcaaagct
    37021 ttgattgatg ctggcgtgga attcaagaca cgtattcgtt ttatctttgg tacagatgaa
    37081 gaaaatttat ggcgttgttt agaaaaatat aacgaaaaag aagaaggaat tacgcaagga
    37141 tttgcgccag atgctgaatt tcctttaatt tatgcagaaa aaggcttatt gcaagcttac
    37201 ttgacaggac caggaacgaa cgagttttct gtaaaagctg gaggagcgtt gaatgttgtt
    37261 cctgatgcag ctccttactc tggagaaaag ctttctgaag taaaagaagc attgaaaaaa
    37321 cacgaattcg atttcgaaga acaaggtgaa ggcatcgtcg ttcttggtaa aagtatccac
    37381 gcgaaagatg cagcacaagg agtgaatgct atttctcgtc tggcaattgc tctttcagaa
    37441 gtatttgatt ttggcccaat caatttctta ggaaaattag ttcaagaaaa tgcaactggt
    37501 gaagctgtcg tgggcaaaac agaagatgaa caatccggcg agctaacgat gaactttgca
    37561 agtttggaaa tcacaccaga acaaacaaaa atcggtgtag atatgagaat ccctgttaca
    37621 ttcaagaaag atgatttagt agctaaatta actaaaacag cagaaaaata tgggttgact
    37681 tatgaagaat ttgacttcct tgattcatta tacgtgccac tagacagtga gttagtgaaa
    37741 aatctattgg ggacatatcg cgatattact ggagacatga ctgaaccgtt tgtatcaggc
    37801 ggcgctactt ttgctcgtac gatgaaccag tgtgtggcat tcggtgcgat gttccctgat
    37861 actcctgact ttatgcacca ggcgaatgaa cgatgggaat taagtagtat gtataaagca
    37921 atggaaattt atgcagaagc tgtgtatcgt ttatgtgcta aataaaaata aacatacaaa
    37981 aagggcggat attcaaatgg atattccgtc tttttttatt tgatgctgga aagtttgcat
    38041 ttctctctta tttttttaag gtataatcat acttgattaa gaaaaagggg tcactcagat
    38101 agaaaaggtg ggagaaagtg aaaatattta gacgaatact aattggctta gtcatcattt
    38161 taggattact cggtggagcc ggttattact atatacagca aaatacgtac gaaccatcgt
    38221 cagaagcgca agtgttggta aaagaaggaa aaaacaaaga agattactta ttttttgaag
    38281 gagagaaaaa gaatcatgga aagtctccat tacttatttt ttatcctggt gctctcgtag
    38341 agccagaaag ttatagtatt tgggcgcaag atttagcaaa aaacggcttt tcagtcgcta
    38401 ttgtcaaaat gccacttgat ctagcagtga tgggcggaaa caaagcggat aaggtcaaag
    38461 aagattttcc aaattccgat tatgtgatcg gtggccattc attaggtggc gtaatggcaa
    38521 gtcgttatgt caataagaat cattcagaca aacatttaaa aggggtattc ttcatggcaa
    38581 gttatcccga caaaaaagga acattgacag aatcagctgt tcctgtttta tcgatcacgg
    38641 cgacaaaaga taaagtattg aatcaggaag cttatcaaga agctaagcag tatttaccgg
    38701 aaagaacaga gtatgtatca atcaatggcg gaaatcatgg aggttttggc agctatggtg
    38761 agcaaaaagg agatggagag gcagaaattt ctaacgcaga ccagcaaact gtgatcgtca
    38821 atacattgga aaactggctg aaaaagttga aataatataa atgggtccta tagggtccgt
    38881 gaaaaaacta gtcaattaac tatagatagc aaatgaatct ttcaaaataa gtcgggattt
    38941 cacggaacaa gagaaacaga tcccggctta ttttcaagtt tagagaactt agtgtacgtt
    39001 tttaaagttt cgtttatatt tatattttat tttctaataa aatcgctcga actggacatt
    39061 tttgatacgc tttttttaca tatgtttctt cttccggcat cacgaactgt tgatgagcct
    39121 ctggttcttg ggcaaataaa acgattcctt cttcatcata atcgaaaata tctggcgctt
    39181 cgatttgaca aagaccgcat gcaatacagc gttcaggtac aagtttacat agtagtgaca
    39241 tttctaacca tcctaactta aggagaataa tatgattgtt tttattttat cgctattttc
    39301 atcaggacac aagctgcgga ttagttctct ttaccaactc cttgtgggaa aacgaacgac
    39361 ttcagtactt atttatggat ttacccacga acttttgttt atccataaca gttttccaga
    39421 attgaagcag gataagtttt atcaaatttt gcaaaaaatt gcgcagcaag gttggatcga
    39481 aataaatgaa aatgaagcaa agctaacgcc tgcaggggct gatatgttgt ccgaacatca
    39541 aatagaacat actggtttgc gttttgaccg ctatggcaga acaggggaaa ctagttggcg
    39601 gctcattaaa tttgctgttc aagtaatttc taatctggca acgggtattc aagactatct
    39661 tccagcagag acaagtcctt tttatacttt ccaactaaaa aaatggctta gtgaaagcca
    39721 attgccacgt gaaattttga ttgatactgc gtatgagagt ttggtgcagc tgttttcgga
    39781 gataccagaa gaagcagcag attttttagc taaccaatta tctggaaatg agcgaacagg
    39841 gctgcttcct tatcagctgg caaaaaacaa tgatgagtca gcggtttatc tgcaacaaag
    39901 cagatgtatc caccttttgt tagcacaaat cgaaaaacgg ccagattcgc tttggcacgc
    39961 tttgatcgat tcattattac agcaaaattt caatcaaagc atgatgatca ccaagcagat
    40021 gtttatgaat ggtcagacaa ttgaccagat catggcaatc cgtcatttaa aaagaggaac
    40081 agtaacagat catcttattg agtgggcctt gttttttgat gattttccat acgagtggat
    40141 tctctctgca gaaacaatcg aacgtttgga accaaataag gattccgtgc gagagtggcg
    40201 ctttagcgaa tggaatgtag atggacaatt agattatgga gaatttcgtt tgtatcaaat
    40261 ctatctattg agaaaggaag cgatacagaa tgttaataaa tgaattaaaa aaatattttg
    40321 gatttgattc atttcgtccc ggccaagaag aaattattca ggcgttgctt gatggagaag
    40381 atactctcgc tattttgcct acaggaacgg gaaaaagcct ttgctatcaa ttgacggggt
    40441 atttaatgga aggtctggtc atcatcgtgt caccgctgct ttctctaatg gaagatcaag
    40501 tgacgcaatt acaaaaacga ggggagaaac gagtagctgc tttcaatagt ttactttcgc
    40561 aatctgaaag aagatacgtc ctccgtcatc tatcgcaata taaatttttg tttttgagtc
    40621 ccgaaatgtt gactaatcct tctcttttag agcatctgaa aaaacaaaat attgcgttat
    40681 atgtagtaga tgaggctcat tgcgtgtctc aatggggagt tgatttccgt ccagaatatc
    40741 aacagttagg aaaaatccga aagcttttag gaaacccggt cacactggca ctaacggcga
    40801 cagcaacaga tttagtagca agagatatac gtcatgtact atttgatcga ccaccaaaag
    40861 agatcagaca atcagtcaat cggcaaaata tctcgctttt tgttcggaaa acgcagcaaa
    40921 aagaacaaga acttgaacac ttcatggaaa gagcacaagg agcggcaatc atttattgtg
    40981 caacaaaaaa agaagtcgaa cgattgtatc atttattcag ggaacgtttt acagttggat
    41041 actatcacgg tggtcttgat gcagctcaaa gaagacaact tcaacagcag tttgtaaaaa
    41101 atcagctgca gtttctcatt gctacgaatg catttggtat ggggatcgat aaatctgata
    41161 tccgttatgt ggtccattat gatttgccag acagtctaga aaactatgta caagaaatcg
    41221 gtcgagcagg cagagatcag gaagaaagtg cagcaattct tttttatcaa agtggggatg
    41281 aacgtatcca ttatttcttt aatcaacttt ctagagaaca gcgacaaagt tttgagcttt
    41341 atttggaata tgccacagag caagctccct ttgatgagct gcaaaaaaaa tggatggagc
    41401 tgatccagca gtctgaaaaa ccagaaaatt gggtggaacg gctcaaacgt caggaaaaag
    41461 aaaaagaatt ccgcctgcag cagatgcttc gctatattaa tgaagaaaac tgccgcagaa
    41521 aattcatctt agcttatttt ggcgaaaaac tctccgaaaa gccgcagaat tgttgtgata
    41581 ttgatggagc aaaagcagaa cttctgccaa agaggaagct tttttctaag actgaacaac
    41641 ttcattggga aacgatttta ctaaatttat ttaaaaaaaa catggaacat tgatttttta
    41701 ggacaagaca ctttttccta tggtataata cacacgatag agtttaggag gtaaaacagt
    41761 gagcagaaaa gatagacatc aatctacaga aaataacgaa acacaagaac catgggaaca
    41821 accaatctac gatacagatg atgaagtttc atcaagaagt tctcagagac aacaaaagaa
    41881 aggaaataca ttatttcttt cgctattttt gattttactg gtcttatgta tcgctattcc
    41941 agcaggtgct tatttctgga tcagaaatgg atcaagcaac aatgcaacag cagcaacttc
    42001 ttctgaaact tcgacatcag tagtagaaag ttctacaagt acaacaaaag aggagtcaac
    42061 tgagacttca acagtagaat ctagtacaat cgcggaagaa tctactgtgt ctcaagatac
    42121 aggtgtaacg tcagaaagca gtattgcagc agagacaaca ccgacttcct caagtgaggc
    42181 aacttatcaa gcaggggatt caagcagcaa tgcagcagaa acaacacctt cttcatcagc
    42241 tgcagccggt tcaacaacga ctgttcaagc aggcgaaggt ccaaaacaag tggctgagcg
    42301 tgcagggatc accactgatc aattgtttga attgaatggc ttagatccaa acaactttat
    42361 gctttaccct ggtcaagaat taagaattaa ataataatag ccgcgctttg cggctttttt
    42421 tctgagaaaa aggagttata tcatgacaca aatcagtatt gcaatcgacg ggcctgcttc
    42481 atctgggaaa agtaccgttg ccaagatatt agcaaaagaa ttgaattata tttatacaga
    42541 taccggtgcc atgtatcgcg cagtaactta tttagctatt acgaacaatg tatctttttc
    42601 agatgaagaa gggctagttt cgctgatcaa aaagtatcct atctcttttt cgcaacaaga
    42661 agatggacag catgtattca tagcatcaaa agatgtgact ttagaaatcc gtcaaccaga
    42721 tgtcacaaaa gctgtttctg aagtctctgc acatggaaaa gtcagagaag aattagttgc
    42781 tgtccaacga aaaatagggg aacgcggtgg cgttgtcatg gacggaagag acatcgggac
    42841 agctgtatta ccgaatgcag aagtgaaaat ctttttagta gcaagcgtaa gtgagcgtgc
    42901 agaaagacgt ttcaaagaga accaagaaaa aggaattccg actgatcttg aaacactgac
    42961 gaaagagata caggaaagag accattatga ttcaacaagg gaagtctctc cgctgaagca
    43021 agcagaggat gctgttcgta tcgatacgac tggcaaatcg attccagaag tcgttgcagc
    43081 aataaaagct gttgttttga aaaaaggtta tcttcttttt taaattaaga aaaaaaatga
    43141 aaaacaattg ttttttcaag gaaaacgaac gaaatcgctt tatttttttt gaataatcaa
    43201 ttaaactaaa aacagtaccg tacttacggc agaatgttgg ttaggaggac aagtgttatg
    43261 acagaatttg atcaaaatca agagaacaac cagtcaatgg aagacgcaat gaaaagcgta
    43321 caagaagtaa aagttggcga tgttgttaag ggcgaagttt tagtcattga agacaaacaa
    43381 gtgattgtcg gaatcgaagg ggctggagtt gaaggagtcg ttccggcaaa agaattatca
    43441 actttgccgg tagaagatat cagcgaagta gtaaaagtcg gtgatgtatt agatttagtc
    43501 gttatcagta cgatcggcaa agataaagaa aacggcagct atctactttc aaaacgccgt
    43561 ttagatgcta aaaaagtttg ggaagatatc gaaagagatt tccaagaggg caaggtcatc
    43621 gaagcacctg ttacaaatgt tgtcaaaggc ggtttagtcg ttgacgtcgg tgtacgtggt
    43681 tttgtccctg catcaatggt ggaagatcat tttgttgcag acttttcaga ctataaaggc
    43741 aaaacattga gctttaaaat catcgaaatc gaaccatcag aaaatcgttt gatcttatcg
    43801 cataaagcgg tagtggaaaa cgaaagagaa gaaaagaaaa aagaaattct ttcaactatc
    43861 catgagggag atgttctcag tggacgtgtt gcacgtctta ctgattttgg cgcatttgtc
    43921 gacctaggcg gaattgacgg tctagtccat gtctcagaga tttcacatag tcatgtagca
    43981 aaaccaagtg atgtattggc tgtcaatgat gatgtaaatg tcaaagtgtt gtctatcaat
    44041 cctgaacaag aacgtgtttc cttgtcaatc aaagacacac agcctggacc ttggtcagaa
    44101 atcgatacaa aagctccagt tggcacagta ttagacggaa cagttaaacg tttgacaagc
    44161 ttcggtgcct ttgttgaagt gttccctgga gtagaagggc tagtccatat ctctcaaatt
    44221 tcacataaac atattgcgac accacatgaa gtgcttcatg aaggtgacga agtgaaagtg
    44281 aaagtattag agatccatcc agaagaacat cgtattgcat tgagtatcaa agctttagaa
    44341 gaaaaaccag cttcagaaga agaaccaaaa aatgttgagt cttatgaatt accagaagaa
    44401 aatactggat ttactatggg agacatttta ggtgatgcgt tgaaagaaga agtagaagaa
    44461 aaagcaaccg atgattctga aaaataattg attttgtatc tgtgactgag atgaaagcag
    44521 ctgcttttgt ctcagttttt cttttgaaaa gcgattagta tttcaataag gaataaaaat
    44581 acttcgttta attgacaatc tatgcttgaa agttggcaat gcattcgtta aactaagtga
    44641 gattggaaac ttgtgaggag gaaataatat ggcaaatcca acaattgcaa tcgttggccg
    44701 cccgaacgtc ggtaagtcta cgatttttaa ccgcatcgct ggtgagcgta tctcgattgt
    44761 cgaagatact cctggagtaa ctcgtgatcg tatttatgca aaaggtgaat ggttaggtcg
    44821 agaattcagc gtgatcgaca caggcggaat tgatcttggc gatgaacctt ttatggatca
    44881 gatcaagcat caagcagaaa tcgcgattga agaagcagat gtaatcattt gtgtagtcag
    44941 cggacgcgaa ggcgtgacag acgcagatga gatggttgcg aaaattttat acagaagtaa
    45001 taaaccagtg attttagcag tcaataaagt agataatcca gaaatgagaa acgatatcta
    45061 tgaattttat tccttagggc taggcgatcc ttatcctgtt tcaggaagcc atggactagg
    45121 gattggtgat attttagatg aagcagtgaa gtatttctct gaagagactg aagaagagga
    45181 cgacgacacg attaagttta gtttgattgg tcgcccaaat gttggtaaat cctctttgat
    45241 caatgcgatt ttaggagaag aacgtgtaat tgtttcggat attgaaggaa cgacccgaga
    45301 cgcaatcgac acccacttcg tttctgaaag tggtcagaaa ttcttaatga tagatacagc
    45361 tggtatgcgt aaacgcggaa aagtatacga aaatactgaa aaatacagcg tgatgcgtgc
    45421 aatgcgtgcc attgaccgtt ctgatatcgt tttgatggta ctgaatgctg aagaagggat
    45481 tcgggaacaa gacaagcgag tagcgggtta cgcccatgaa gctggtcgtg gaattattat
    45541 cgtcgtgaat aaatgggatc ttgtaaagaa agaaaccaat acaatgcgtg atttcgaaca
    45601 agagattcgt gatgaattcc ggtatcttga ttacgctcca atcgtttttg tttctgcagt
    45661 gactaaacaa cggttggaac gtctgcctga aatgatcgaa caagtaagta tgaaccagaa
    45721 tcttcgtatc tcttcggctg tgttgaatga tatcattatg gatgctgtag caatcaatcc
    45781 aacaccaaca gataaaggaa aacgcctgaa gattttttat gctacacaag tcgctgtcaa
    45841 accacctaca tttgtcgttt ttgtcaatga agaggaattg atgcattttt cttatgaacg
    45901 tttcttagaa aatcaaatca gaaaagcatt tacttttgaa gggacgtcca tccgaattat
    45961 ccctcgtagg agaaaatagg cattttacca tatcgacgtt tttttttcaa aagaaaaaga
    46021 cagtttcgtt tgttacgttt ctatgactaa aaaaagcatg tgaaaattgg taaaaacctt
    46081 gctattaagc ggttcatatg atatcgtgat aacagaatat tgaagttaat caatatttct
    46141 ttttaaactt ctggaccact cagaaagttt taactaaatg atatttctat catatctctt
    46201 gtggaggagg tgaatctatc catggcaaac aaagcagaat taatcgaaaa agttgcttca
    46261 gctactgatt taactaaaaa agatgcaact gcagcagtag atgctgtatt ttcaactatt
    46321 caagatgctt tagctaacgg cgaaaaagtt caattaatcg gttttggtaa ctttgaagtt
    46381 cgcgaacgcg cagctcgcaa aggtcgtaac ccacaaactg gtgaagaaat cgaaattcca
    46441 gctagcaaag tacctgcatt caaaccaggt aaagcattaa aagacgctgt taaataagac
    46501 agttgaaaaa gcgcttgtgg ctctagggct acaagtgctt ttctttttat ttcctagcag
    46561 aaaaggaata aataagagac agagattccg aatccgtatg tcatacatag aaaacacatg
    46621 tattttattt aatttttatt tatttgcaag ttatgaggtg agctttgctc ttatcttttc
    46681 tagaatttgc ttactaatgg taaaataaaa ataagaagag gagggatgcg tatggaactt
    46741 gattatcaag tagaagatgc gttgacgaaa gtgttttacg gagaaacgct agtcagtcag
    46801 ctaagagaac tttccattga tcagccaatc ttatttttaa cgaatcagcg ctattatgat
    46861 ttgtttgcag ataagatcaa cccgttattt gcaaatcaag caaacaatga ctggtatatt
    46921 tgtgccaaca ctcaatgcaa tcatttaaca gaattaaaga acctgcttga tttcgtcaaa
    46981 cgctatcccg aaaatcaatc catgtttatc atcggatttg gaaatgaagg gatcatggat
    47041 ctggcaggtt tttttcaaaa acatacaacc cttgattcat cactatggat gatccctgta
    47101 tcgatccgtt ctctaagccg agcattaaca ccgcagcgga cgattttgca gctaccaaat
    47161 cgtgtagtgc tgcaaaccat caatctgcca gaacgcatcg tttatgatca aacaatcagc
    47221 gagcggcagg tggacggaaa gcagattgac tttttgactt ttatctgttg cggaatgatt
    47281 tgtgatcatc gttttttgca aaatctgtat aaaaactatc caaataaaaa gcaattaacg
    47341 actcggccgt ttgccgggat gatcgaagaa atgatctatt tttatcaatt agaggcagaa
    47401 aacctgtcaa attatggtaa actgtttgaa caagcttttt atctgaccga aaatggccat
    47461 ctactttctg ctagtatgaa aaaattttta ggaatgatgt ttcatttcgt gtggaatctc
    47521 caagcatatc cttttgattt ccaactaaaa aacttttttg tgtggctgga cttgcttggc
    47581 tatccaatcc actggctgga acagatttca aagatggagt atttggaaaa agttcttttt
    47641 ttggcagaaa aaagcaaaaa aatacttgtt ttagaaaaaa taggtataat agacggatac
    47701 caacatccaa acgaaaaaga attattgaga acaatgacgg tatttcaaca aatcgtcagt
    47761 gagataagag ggaattgaaa tgaatacaaa tagcgaaaaa atgttgcaag ctttatcaga
    47821 tgaagacttg tcacaagcac aaatcttttt agaaaaagca ttaaaagaag acccagcaga
    47881 tatattagct gaactaggcg aagaattgct ggctattggc tttttgatcg aagcaaaaca
    47941 gatttttgaa caactgatca accaataccc agaaaatgac ggactgaaca tcccgttagc
    48001 agaaattgca atcgaagata atgagatcga cttagctttt gaatacctgg aaaaagtgga
    48061 taagcaaagt gattactatc cgcaaagctt attggcgata gctgatttgt accaagttat
    48121 cggaatccct gaagtgagtg aagcaaaatt gaaagaagca gctgggttgc ttcctgatga
    48181 accattgatc cagtttgcgc tagctgaatt gtatttttca gttgatcgtt tcaaagaggc
    48241 agcagtggtt tatgaaacat tgttggcaag cgggataaat gaaatttcag gaatctcgat
    48301 gcaggaacgt ttaggacaat ccttaagtat gcaaggagaa ttcgaagcag ctatcgtacc
    48361 tttagaagaa gcactgaaag aagaaaggac agatgatcga ctgttccagc tagcttttac
    48421 gaatcttcaa ttgaaagaaa atcagcaggc aatcaactat ctggaagaac ttcgagaagt
    48481 taatccgcat tatcaatcgt tatatctata tctaggacaa gctcttcaag aagaagaaat
    48541 gattgaagaa gcacaaacag tgttagaaga aggaatcaaa gaaaatcctt accaagtcga
    48601 actttatcac ctggcctcgg aaaatgcttt ccgtctgcat gatagacagc gagcagaaga
    48661 acttttactg aaagctttag aagtaggaga caaacaagat gaaacattac ttactttaag
    48721 taatctttac ttggatgatg aaagatatga agatgtgata caagtgatca atcagatgga
    48781 agaaactgct aatccttatg cagaatggaa cctagcccat gcttataatg aattagaaga
    48841 ctttaccgtt gcagctgtac actatgaaca ggcttatcat gaactatcac atgaaccaga
    48901 tttcttgaaa gagtatgcct ttttcttaag agaagaaggg cagctgaaaa gagcgcaaga
    48961 attattgact tattatttaa cacttgaacc aggagatatg gaggcactgt cattattaga
    49021 tgacctaaca gagaggtgag aaaaatgcta attgatgtac gggaaaagaa gaatttttta
    49081 acgtggatgg tcaatcatgt atcttttagc cgaagagaag ttttttggat cttgaattat
    49141 cttgccaatc atgaagcgat tttaagcaat gtccactttg ttgaaggggc gaattttgcg
    49201 gatcgaggac tgcagatcag tgatctatct gttgaagggg aacccatgaa attgtttttg
    49261 cagggaaaag aatttacgga cacagaccag atttttcatg aaatccgttt gaattggaaa
    49321 aagcctttat atgtggaatg tatattcgaa aatgcttggc agacaagaga atatctatct
    49381 atcttagaag acaatccatt ttcttcttgg gattcttcag tcagtgaaga agatactgaa
    49441 ttaattgaac gatattttca agaaaaagaa gaagaggcaa aactaaaaat gctttatgct
    49501 caaatcgatc aagcactaga agatggagat agggatgctt ttttgaaatt atctgatgaa
    49561 gttaaccgaa agatcctttt gcagacacaa aagaaaagta accgggtaag tgaacataac
    49621 aattagttaa aaatagtata gtactaaaaa agacaaaggt cagctggatg tggaatctt
//
LOCUS       DACDYJ010000016        48739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDYJ010000016 DACDYJ010000000
VERSION     DACDYJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.016"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            23..646
                     /locus_tag="IWM51_000885"
     CDS             23..646
                     /locus_tag="IWM51_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970321.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IWM51_000886"
     CDS             1096..2145
                     /locus_tag="IWM51_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9970322.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IWM51_000887"
     CDS             2142..3023
                     /locus_tag="IWM51_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9970323.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IWM51_000888"
     CDS             3026..3796
                     /locus_tag="IWM51_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9970324.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IWM51_000889"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IWM51_000889"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP9970325.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IWM51_000890"
     CDS             5107..6270
                     /locus_tag="IWM51_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9970326.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IWM51_000891"
     CDS             6387..6797
                     /locus_tag="IWM51_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9970327.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="IWM51_000892"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="IWM51_000892"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9970328.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="IWM51_000893"
     CDS             9337..9654
                     /locus_tag="IWM51_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970329.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9814..10050
                     /locus_tag="IWM51_000894"
     CDS             9814..10050
                     /locus_tag="IWM51_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9970330.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="IWM51_000895"
     CDS             10047..10445
                     /locus_tag="IWM51_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9970331.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11112..12056)
                     /locus_tag="IWM51_000896"
     CDS             complement(11112..12056)
                     /locus_tag="IWM51_000896"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9970332.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12352..13296)
                     /locus_tag="IWM51_000897"
     CDS             complement(12352..13296)
                     /locus_tag="IWM51_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9970333.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13523..14716
                     /locus_tag="IWM51_000898"
     CDS             13523..14716
                     /locus_tag="IWM51_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9970334.1"
                     /translation="MNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAVEL
                     LTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASRAG
                     LGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQYT
                     FLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIVVY
                     NAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSLLL
                     MGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPTNTSILLVGANLGT
                     FIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIRVDEEIKE
                     QLTTK"
     gene            14738..16753
                     /locus_tag="IWM51_000899"
     CDS             14738..16753
                     /locus_tag="IWM51_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HAP9970335.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16836..18209)
                     /locus_tag="IWM51_000900"
     CDS             complement(16836..18209)
                     /locus_tag="IWM51_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9970336.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18235..18519)
                     /locus_tag="IWM51_000901"
     CDS             complement(18235..18519)
                     /locus_tag="IWM51_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9970337.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18521..20536)
                     /locus_tag="IWM51_000902"
     CDS             complement(18521..20536)
                     /locus_tag="IWM51_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9970338.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20715..21020)
                     /locus_tag="IWM51_000903"
     CDS             complement(20715..21020)
                     /locus_tag="IWM51_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP9970339.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21109..22140)
                     /locus_tag="IWM51_000904"
     CDS             complement(21109..22140)
                     /locus_tag="IWM51_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP9970340.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22347..23222
                     /locus_tag="IWM51_000905"
     CDS             22347..23222
                     /locus_tag="IWM51_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9970341.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23374..24216)
                     /locus_tag="IWM51_000906"
     CDS             complement(23374..24216)
                     /locus_tag="IWM51_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9970342.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24308..26185)
                     /locus_tag="IWM51_000907"
     CDS             complement(24308..26185)
                     /locus_tag="IWM51_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9970343.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVCNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26182..27603)
                     /locus_tag="IWM51_000908"
     CDS             complement(26182..27603)
                     /locus_tag="IWM51_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9970344.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27606..30995)
                     /locus_tag="IWM51_000909"
     CDS             complement(27606..30995)
                     /locus_tag="IWM51_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9970345.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31252..32643
                     /locus_tag="IWM51_000910"
     CDS             31252..32643
                     /locus_tag="IWM51_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9970346.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32716..33363)
                     /locus_tag="IWM51_000911"
     CDS             complement(32716..33363)
                     /locus_tag="IWM51_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9970347.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33371..34057)
                     /locus_tag="IWM51_000912"
     CDS             complement(33371..34057)
                     /locus_tag="IWM51_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9970348.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34063..35136)
                     /locus_tag="IWM51_000913"
     CDS             complement(34063..35136)
                     /locus_tag="IWM51_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9970349.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35358..36032
                     /locus_tag="IWM51_000914"
     CDS             35358..36032
                     /locus_tag="IWM51_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9970350.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36037..37377
                     /locus_tag="IWM51_000915"
     CDS             36037..37377
                     /locus_tag="IWM51_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9970351.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37526..38512)
                     /locus_tag="IWM51_000916"
     CDS             complement(37526..38512)
                     /locus_tag="IWM51_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP9970352.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            39236..40267
                     /locus_tag="IWM51_000917"
     CDS             39236..40267
                     /locus_tag="IWM51_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9970353.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            40376..41908
                     /locus_tag="IWM51_000918"
     CDS             40376..41908
                     /locus_tag="IWM51_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9970354.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            42047..42859
                     /locus_tag="IWM51_000919"
     CDS             42047..42859
                     /locus_tag="IWM51_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP9970355.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            42849..43334
                     /locus_tag="IWM51_000920"
     CDS             42849..43334
                     /locus_tag="IWM51_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9970356.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            43357..43902
                     /locus_tag="IWM51_000921"
     CDS             43357..43902
                     /locus_tag="IWM51_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP9970357.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(43975..45618)
                     /locus_tag="IWM51_000922"
     CDS             complement(43975..45618)
                     /locus_tag="IWM51_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9970358.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(45747..47975)
                     /locus_tag="IWM51_000923"
     CDS             complement(45747..47975)
                     /locus_tag="IWM51_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9970359.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(48008..48487)
                     /locus_tag="IWM51_000924"
     CDS             complement(48008..48487)
                     /locus_tag="IWM51_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970360.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        14832 a         8979 c         9166 g        15762 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc
    10741 catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg
    10801 tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt
    10861 agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca
    10921 ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga
    10981 tacattttcg gaccgattaa ttttaaccat tctgtttatt tgcttaccta ttagatatca
    11041 ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt ttagttcata
    11101 ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt tgatttgctg
    11161 aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac accattttcg
    11221 tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc agttaaataa
    11281 gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat acgtgcagta
    11341 ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata agcagcatta
    11401 cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc tttttctaca
    11461 atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac ttctgaatca
    11521 ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg
    11581 atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac acgtgatgtt
    11641 ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt tgaagcaaca
    11701 acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg catgattgac
    11761 gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat accagctgtg
    11821 ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac agccacattt
    11881 tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc tttttcttga
    11941 ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc ataagcgata
    12001 cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt tttcatgaat
    12061 aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct tgtttattca
    12121 ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa acgaattcct
    12181 gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat aatacaacca
    12241 aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa taagacagca
    12301 atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt cttaatgttt
    12361 atttctccat tcattgggag tacatgttga atacttcttg aataatttga ataaagaagt
    12421 cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg tttcttgaat
    12481 cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg aagcatcaaa
    12541 atacattttc aatttattac taagatagac cggattgtag ccaaaatgag ctgctgtttc
    12601 ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa gcatctcatt
    12661 ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag tcaattcgat
    12721 cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg tatcaaaata
    12781 aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg
    12841 aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag aagaaagtag
    12901 attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt cttttaataa
    12961 gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag cttctttatc
    13021 aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa tttgtaaaca
    13081 tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc
    13141 atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc ctcggtggat
    13201 ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca ttccttgttc
    13261 acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt
    13321 ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca cgctgtactg
    13381 ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact
    13441 tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga taagcgctta
    13501 caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt
    13561 ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac
    13621 ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct cttttatggt
    13681 cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt
    13741 gatcatcgga ttagtgattg catcgattta tggtatcctc cccctcttct cacaaagtta
    13801 cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt
    13861 ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt
    13921 tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag gacagctagt
    13981 acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta ttcttgtctt
    14041 gtttgttttg tttgttccta cacctgaaac aactgaactt caatcacatg gcacagcccc
    14101 aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct
    14161 ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc
    14221 tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc
    14281 tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc
    14341 tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat
    14401 tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa
    14461 ccaagtaagt atccaagcgc caaccaatac ttcaatctta ttggttggcg ctaatttagg
    14521 tactttcatt gcaccatatg gtttgctctt tctctctttt gcttctcttg ggagtaaaac
    14581 acttgctgtt ttcgtcaatg gcagtatctt gctgttgatt agcgcgctta ttagccttct
    14641 agtagtacac aggacgaata gaaagacagt gataagagta gatgaagaaa taaaagaaca
    14701 attgacaaca aaataaattt gatggaggtc gtgtaaaatg acaaatgaaa aacataaaga
    14761 tagatggctt tggtatcctg gagactttga gatccgccat ggactgctac aaaatttcca
    14821 acgggaagaa cgaggttttg actggccagc gtactggtac atggatgatt gccatcggaa
    14881 cgtcaagttt aagagatatt actttcttga tcaaccttcc atgtttaaag tgacgatcca
    14941 aggagttggg tatgtagaaa ttaatggaca aaaacatcca tgtggaaaat ggctgacttg
    15001 tccagctggt aaagcaaaaa ttcgtatctt tgtaggacat acatcagggc taccagcgat
    15061 gtttatcgag ggagacgaag taaaatcaga tattggctgg acagcaagta attttattga
    15121 agaatatcca gctggctgga gtccgcttta tgtcgatatc gcaaaagatc caaatcaaat
    15181 ctattatcaa aaagaatgcg tccatccagt cactgaaacg gagattggcg gaggaatctt
    15241 attcgatttt gggcgagcag tgaatggctg tgtagaacta atatctctgg atgaccatat
    15301 cgaatctatc actctatgtt atggagagtc cgatgtagaa gctatggata tcgattattg
    15361 ctactataag caaataggaa tgacaagaaa aatgaaagct cgtaaacgcg cttttcgcta
    15421 tttgtttata ccagaggttt ctactggaat ggttcaactt caagtttttc atgaattttt
    15481 atctctttcg aaacaagctg ctttttcaag cgattcttct ttactgaatc agatctggtc
    15541 agtatcagaa gaaaccttca agttatgtag tgatttgttt tttatcgacg gtatcaaacg
    15601 agatcgctgg atttggtctg gagatgcgta tcaatcattc atgatcaatc aatatctttt
    15661 ttttgacgag gaaatcaata aacgaacgat attagcatta agaggacaaa atgaaatcaa
    15721 gcagcatatg aatactatcg ttgactattc gatcttgtgg ttgattggta tcgaaaacca
    15781 ctatatgatg acgaaagact atgaattttt acgcatcgtc taccctaaga tgcaatcatt
    15841 aatgagatac ttgatggagc aaacgaatga attagggttt atttatggga gagcaaaaga
    15901 ttggatattt attgattggt ctgaaatgga taaagaagga acgcttgctg cagaacaagt
    15961 cttattactg aaagcctatc attccatgat cacttgcgga gatatcttag ctgaaacata
    16021 tggagagaac gaaaattatc atactcatct ctatcaagaa aaatatcaac tattgaaaca
    16081 aaacctattt gactactttt gggatgaaga acttggcgct tttatcgatt gctatgaatc
    16141 tgggagaaga aatgtgacgc gtcatgcgaa tattttcgct gttttattcg attttgctaa
    16201 caaagaccaa cagcaatcga tcttaaagaa tgtcttattg aatgacacta tcacacagat
    16261 tactactcca tatttcaaat tctatgaaca agatgcttta tgcaaactcg gacagacgaa
    16321 tatcgtctac caaacgattc ttgattattg gggagggatg ttatctcatg atgcggtaac
    16381 tttctgggaa gaatacgacc ctactcaaac aggaaatgaa aagtacagta tgtatggaga
    16441 tccttatgga aaaagcttat gccatgcatg gggagcaagc ccaatctatc ttttaggacg
    16501 ctattttatc ggtttgtatc caacaaagcc aggttatgaa gaatttatga tacaacctaa
    16561 tctagattca tttgaaacac tgaattgtac gctacctatc aaagatggaa agatttcgct
    16621 tgaaaaaaca aaagataaga tcgtgattac ttctgataga acaggcggaa cagtgaaaat
    16681 gaatggtaaa agttatcctc ttctagcaaa acagccactt gtcttaacaa gctattcaaa
    16741 aataaatgca taaaacaaaa aactgcttgc gacatatcag aagactttaa atcttcttct
    16801 gatattgtcc aagcaatttt tattttgaag tttgcttaat tctgttcttt agaaacaatg
    16861 tttgttcttt ttccggcaaa aagaaagcta gataatacga caagtaaaaa gaaagcgatg
    16921 attccaactg tcaaggctat ttgaccgtat tctcctagtt tcccaagtac aagtcctggt
    16981 actagataat cagtatcggc aaatgtagaa gaagcgattt taagatctcc taatactgga
    17041 aggataaata atggcagcca actaatcaat aagctattga aagcagaacc taataccgcc
    17101 cctcttctac cgccagtcga gttgccgaag acaccagaag cagctccgca gaagaaatgt
    17161 cctactaccc caggaatgat aactgtagtt tgcaagccta tcatagcaaa catggatagt
    17221 gttcctacaa agaagctaac aaaaaagcca attaatacag cattaggtgc atatggaaaa
    17281 acgataggaa catctaatgc tggtttagaa tcgggtacga gttttttagc aatcccttgg
    17341 aatgctggaa cgatttctgc caacaccatt cgaacccctt gaaggacgat aacaaaccca
    17401 gctgtgaatg ttccagcttg gatcagtgca aagattacag cgttgtctcc attgcttagt
    17461 tctctttcaa caaatccagg accagctaac aaagcaagtg cgacatatac gatcgacatc
    17521 aagagcatga tcgatacagt agaatctcgc aaaaagctga aacttttagg aatctttatg
    17581 tcctctgtag aacggtcttt gtttccaaaa aactttccga tccatccact agaagcataa
    17641 ccgatatttc cggtatgccc taacgcaact tgattgtttc cagtgatgtt cttcataaac
    17701 ggttgacaaa gagcaggcgt aatagttaat agtgttcctt cgaagacccc gcctaataag
    17761 atcatccaca aatttgcagt aaaccccgcg cttactaaaa tgactgctgt tagacaagaa
    17821 acataaagca ttgcttgacc tgtaagaaaa atgaatttat atcttgtgaa tctagcaagt
    17881 aaaatattca cgatcatccc tactagcatg atcaaagcag taggtgtacc gtattgttgc
    17941 aaagcaatcg ccactactgc ttcattgcta ggaactactc cttgagtatg cagagctgtt
    18001 tggaacatcg aagcaaaagg ggttagtgaa ttactgatta aaccagcacc gccgatcagt
    18061 actaggaatc cgacaaatgt tttgatagtt ccttggatca cttctgaaaa tttcttcttc
    18121 tgaagaacga gaccaagcag tgcaatcaaa gaaacgagga tggagggctg actgacgatt
    18181 gacacaaaca aattcaacaa tttatccatt gatagttcct cccttgaatc atttttattc
    18241 ttttgacaag atcttcgtta ctttttcttt tacttcttcg ctgtcaatga tcgattgcaa
    18301 aggtacgagc ttttcatcag gtatagaagt tagcgtatct ttcaaagaat gctcaatgaa
    18361 gaaataatca gccatatcgc tagatacact tccaacatcc gtatgactta cttcaaactc
    18421 gtccacatct gctccaagat ctgcaagtgc ttgtttgatg ttcatctcta ccataaaact
    18481 tgttcctaat ccactttgac atactgcaaa aattttcata tcattcgtct ccttttctta
    18541 tgatcgctaa tatttcttct tttgttgatg cgtgtaataa atcttgaaga ttttgtttat
    18601 tagaaagtag ctttgttaaa tttgccaaag cccgaagatg tgcctcatta tcgatagcag
    18661 ctaaacagat gaacaaacta atgggatggg ctggattatc aagcagatcc acaggttgtt
    18721 gaagtttcag caaagacatc cctacttcgt ttacgccatc ttctggacga gcatgaggta
    18781 atgcgatgtg atcgccaata tggataaacg caccatactg ctccacgcgc tcgatcatag
    18841 ctttgatata actttcttct attttatttt ctgataatag cggctgtgcg gcaaatgtga
    18901 ttgcttcttg ccagtttttt ggttgatccg ataattggat catttcttct gttaataatt
    18961 cagatagcat aggacgttga tcctccttct tttctaaaag tttgttcatt ttacgattta
    19021 atacttgata gagcttttct tcgtctacgc cttcttttaa ttcgatataa ggcagtagcg
    19081 ccgacaacaa ttcttgggca gaaggcattg aaaatcctgg tatcagccaa tctctttgta
    19141 cttgattgat cagctgattt ttttccaagt cagtcatgat tggatgcaga acatagagag
    19201 actttttctt atcagtaagt gtgagaggta cagtagaaaa aataatatcg taagaagttt
    19261 cgtcaacctc gtgtaagcgg tcagctgaat tagtctctgt aaaaaagatc atcgggaata
    19321 attgctgaag ttcacttttt aagatcaatg aagagctaat gccacttgga cagacgatca
    19381 cggctcttat ttttctattt ctttcttctg cgtccgcttt tctgatctct ccgccaaata
    19441 gtatagtgaa aaaaccaatc tcttcttcag ggatgctctt ccctattcgt ctttctaacg
    19501 gaagtaatgc ttttcttgtc atttcgtaga tggaagcata ttgatgctta atttgatcga
    19561 tcatcacatt aggtaaataa aaagaatagg aaatacgaaa ataggcaggg accaagtgat
    19621 aaaagacatt catcaataat tcacgaaacg aagcaaattc tacggccatc aggaattcca
    19681 tgcgatggat gacttctgaa gaacattcca gcaaaaaatc aagtgcaggc tcctggacat
    19741 ctccttcaac gatcgatgct aagcaaagag agaaaaatat aatctccgaa ttttgatgtt
    19801 cggaatagcc aaggccagtc aatacttgtc gactaatgtc agtgactgca agagaagcca
    19861 acagttcagc atcttttacc agttccatgg atgtttttcc aattcgaatg gttgtcaaac
    19921 tcaaataata agctaattca tccattcgat ttggtgcgaa tcgaagatct gatagtgaaa
    19981 tcgttttatc tagcaaatct ctaactttgg cgtaataatc aatggaaagg gttgcggtta
    20041 aatacgtgag cccaaatctt ccagcaagtg actgcatcaa acgagcagtg cgattttttg
    20101 cttctcttct taatattgct tctgacccta gcagcctaaa accagttttc ctagtatacg
    20161 acagctgaca atcttccact tctaattcat ttcttatctt tttgatatct gacaggactg
    20221 tccctttaga aacctgaaga aaatcttgaa aatgaaaaac agaaacagac gttgcagtcg
    20281 aataaaccat caaataaaga agatctcttc gctcttgttc ggtatacaca gcttcaaatc
    20341 gagtaatatc caaaaatttt tctgcccagt cgattgctcg gaatttcttt atacggatac
    20401 gggtatcatc cacatattcg gcaaaaggag ttaattgttt gatcgcctcg cttgtattta
    20461 cttcgatgat ctctgctgct ttttctagac tgatttctct tggtgttttc aggagataac
    20521 taattttgta gttcacagcg ctgttcacct cctacttata ttctagtatg gtagcgcttt
    20581 taaatcgagg ggtgtttggt acaacttaca gttggatttt ttttacgact gtaccgaacg
    20641 atttctatcg aatggataca gaaaaaaagc aggtgaccaa atgtctcctg cttttatgaa
    20701 ttgatttcgt ccgatcaatg atgatcatgt ttattttttg aggtcagttc ttcaaatttt
    20761 tctttgacat cttcaaccgc tttttcgata tctttttcaa agtgattggg ctcattaggc
    20821 tttgtttcct cttctacaga atttgaacca gataaagctg tttctttatt ttctgtttca
    20881 ccagagtttg tgccagctaa tgattcttct ttgtcttctg tttcaccaga attagttcct
    20941 gctaaagcca cttcttcttc tgttttaggg gccattgaac ctcttaatcg tttgtgtttt
    21001 tcaccgtcat aatttgtcat gttggttcct cctagtagtt ctatactttt tcatcgtaca
    21061 ataagacagt ttatgcgtca aaaaacaaac ccgattctta caaatatgtc atatttttcc
    21121 ttctgattct tgaatgcttt tgattccttc ttccaaaatt ttcatttgta agattgtttc
    21181 ttcatctttg atgacttttt ctgcgccatt aactaatgtc tcgtaaatcc cttcgtagac
    21241 acggctgtaa tcaccaacct cggaaattac tttttcttca tgataactcc cttgttcatc
    21301 tatataggtc aatacaccat aatgttcggg caaatcgata ccaaagtctt catgatcagg
    21361 catgtaaaac attttcagat gttcttcttg tcgatctttt gtttgtttca cgaacatccc
    21421 tttttttccg taaacgacga aacttggacg ttctttgatg cggaaataac tggattttat
    21481 cgaaattttc atatttccat agaaaaaatc cagatcgaag taatcattca ttcgtccttt
    21541 ccctaaaagt tgacgtacat catagctgat atcatctggt tcgccgaaat aagaaagaac
    21601 ttgatctact gtatgacaag catgtccata taaatagctg ttcgcagtag aaaattcatg
    21661 cgtattttct ggtacttccg gtctgaaata atcataatgc atttctactt ctaaaatatc
    21721 ccctaatttt ccgctttcga ttactttttg gactgtcaag aagtcagaat cgaaccgtct
    21781 gttttggtaa cattggataa atagaccttt ttcttttgct aatgcaaata actcttctgc
    21841 ttcctttgac gtttctgcaa aaggtttctc tactagtaca ttcttaccat gttcgagtgc
    21901 catttttcca tattcataat gaaattgact aggtgtagtg atgacaacta attggatctc
    21961 aggatcatcc caaagatcag acagctgatc agtatattcg atgtcttcga atgttttcca
    22021 cactggttca gatggggaaa aaattttttt gatattgatt ttgttcgtaa gttttcttga
    22081 aaaaggcaaa tgatagcgat ttgtactctt tccattgcca agataagcga ttgtaagcat
    22141 gagtggtgcc tccttttatt gatgttttca gtatatccta gaaaagcgca gaaagtattt
    22201 ataatagcta ttctggtaaa aaagttcaga aaaacacttg ttatcattcg gtttggtgaa
    22261 attaatgttc aaagtaagga taaaaatcaa aaagatagta gcggaaccaa gtgtttatca
    22321 gtaagataga atttggaggt cagatcatgc ttattgaaga aaaactaaca aaacaagagt
    22381 tgtttaccac gacagagaaa cggattgtcg attacatccg taggaatatc gaagctgcag
    22441 tctacatgac gattgaagaa ttagcaaaag ctacgtatac ttcccattca gctatcattc
    22501 gtttatgcaa aaaaatggga ttcagcggat ttaaagaatt ccgtttcgag ctggggcggg
    22561 aagttcatca attgattgct cagttcaatc aaacgattga tcctaatttt cctttttcaa
    22621 gtgaagatag cactcagcaa attgcaaaga aaatggcgga tttatcgatc cagtcaataa
    22681 aaaaagcaca attgcagatt gaacatgaag atttagatca aatcggtaaa gtattgaaca
    22741 aggcacagcg aatctttctt tttgcaaaag gtgattctca aatcacggca aggaaattcc
    22801 agaataaaat ggtcaagttg aataagtttt tgatcatggc tgaagaatac agtgattctt
    22861 cttggaatgc agccaatttg actagcgagg attgcgccat ttttattagc tacagcggtc
    22921 gcatccatca ttatgaacga ataatgacct atttgaagca tgtagaggcc cctaccttgc
    22981 tgattaccgg aaatcagcat tcagaaatgg ccaaacaggc tgatatgtgt cttgttatat
    23041 cgcaggagga atatgatttt gctaaagtag cgactttctc ctctcaaatt gccttcgatt
    23101 acgtattaaa tacgttattc tcagtgatat attctcaaaa ttttgaagag aatatccgca
    23161 acttgaaaaa caaacaagaa ttgatgcaaa aaggccttct taaagaatat cggaaaactt
    23221 aagaaaagat cctcatttag taataagaaa gatagtgttt gataggctct ctttgatatc
    23281 atcataaaaa caggctgaag ttttcttttt caaacctcag cctgtttgtt tacggaacga
    23341 ttcgaataga tatattatag aaatgaggaa tagctaactt ctgtttctta ttttgaacga
    23401 ttgacgaaaa agatatataa gaaggaggaa aatcagtata catgcagcta acaatccata
    23461 caggaaaaat cgtttgttct cggatacttt ctgtacttct ttttgaactt cttttgcatg
    23521 ataaggaacc cggtgccctc tgacaagtaa gcgatgactg ttgaccatat aaggtgtaca
    23581 agttaggagt gtcacgaaat cctgcccttt ttcgattctc agatcttttg tatcagtcgg
    23641 ttctaccgtc ttgatctgat cgacttgata agcgagtgtc cgtccattga tttcgatata
    23701 gaaagaatca cctaaagtca gtttcggtaa gtctgtgaac aactttgctt gaggcagtcc
    23761 tcgatggctg gaaataacgg catgtgtatt ttctcccccc gtaggataag acgttccttc
    23821 caataatgag gcaccttttt ctagcagcac agtattcgtt tcatcaaaaa ttggcaaatg
    23881 gacattgatc tcagggatcg tcaaaatacc gatcgtatgt ttctcaaaat aggttctatc
    23941 ctttttcgca ggttcttttt tcttagtaaa gggatctgca cctggatttc caccttcttt
    24001 tgctaattgc tggtttttct ttgtcatgtt ttcttggatt ttagccatga cttcttcatt
    24061 ttctttgacc gcttgttgct gataatgaga gatgatctgc tgatccagat aattattcag
    24121 cgcgtcgcta acaaatggat aagctagtat ccctattcct gagagaagca agaaaagcat
    24181 cagcagatct agaagtttcc ttttctttgg tttcatttgt gttctccttg tgatgactgt
    24241 ttgataataa aagaaaaagg agcatttgcg taatccgctg ctcctttttc caattcttgc
    24301 aaaaagacta aatctgactg tgcttgcgtc tagcaaagta cagtccagca atcagtagaa
    24361 ggactactcc tgcaccgata tacacataga ttcccttacc gcctgttgaa ggaagtgtac
    24421 ctttgtgttt atttggtatt ttttcaggag aaatcaactg tcctgctgtt acataagatt
    24481 gttcatcgat agtaaatgct acacggtttg ttaatggaac atatttttct ggcgctttcg
    24541 tttcttccag atagtacgtg ccatatttta gacctgtcac atcgattaaa ccgttacttg
    24601 tggttgtaaa aaccgttgct gattctttcg ccgataccca gctgacggct tttgtggatg
    24661 ggtcgatcga taaatatttc gctgtgtcac tatcttgatc acgaacgacg aattctgctc
    24721 cagcaagtgt ttggtctgat gtaacgtcac catctacttt aacaaatcgt ttgcccccag
    24781 taacgacatc gactgacggt ggtgtttgat catttgtatg cccgttatcg acatttgctt
    24841 ggttagaaaa tcctttggtt ggatctgctt tttcgttcaa atgcatatag taaacgaatt
    24901 tcaatgtacc gccaggagtt aatgaaggaa tataattcgg atcaactgaa accgtgaatc
    24961 cgtctgtttg ctcagtgaca gaataattta ctgggtcgat ttctttattt ccatcatata
    25021 aggcataagc atacgttcct gaagaatcat tatcaaatgt taaagcagcg tcatgagtat
    25081 cgataagttt gaatgttgtg tacttgtttt gcgttccttc tttatcagcg atacctaatg
    25141 ggatattgac agaaatctca tattgaattt tttcaccgat tgcgacatct tttccattca
    25201 attgaggtgt tgttttatca actttagacg tatcattttt gatggtcttt tctactggtg
    25261 tttggctatt tgccaggatc tcaaaaggcg tgattgtttg cttttcaatc atttccgcat
    25321 tatcaggagc ttttacttct tctaaattat attttccaac ttctaaatga ttaacgatca
    25381 actggccttt ttcaatagat gcctcggcaa agtcattgtt gccgatgtca taagaatgac
    25441 cagtaatgaa atgtttggct ttggtttgat cagttgtcca agtgtacaat ccatctgtga
    25501 cactttggat gtattttttg acgcttggtg ttccttcttc tttagaaata ataaattctg
    25561 ctccatttag tgcttcgttt tcggcagtac cgatttttgt aactttcaac gttccatcat
    25621 tacagactgt atttttaggg tagagatgga tagtatctag ttcttctgtc ccgtatttat
    25681 aagagccatc tgcttgtttg atcatctcat atacagggaa agctaaaacc atgtttgcgg
    25741 cagctgacac tccgtctttt ggttcttctt tgatcgtgta gactgcatct ttcccatttt
    25801 gttttttagg taaagataaa gtgacatttc catcagcatc tgtcgttcct gaagcaactg
    25861 gtgtaccagg agtcaaagac tggactgctt gttttgctgc atccacggac gctcctttat
    25921 ctcgttgcgc ataaaattct tgagtgacgt tataaactga aaatgaaata tcggctaatc
    25981 cttggtattg atcgaattcg ctcatttctt tcccgctgtt ttggattaaa ggatcgggta
    26041 aatcagtcat tttcttttta tgaaggatga cttgagctgc tgtctcctct gcaaacactt
    26101 tttttgcgcc gaagctgttt gtgagtaatg gtaaaataag gaaaaggact cctaacataa
    26161 cgtttatttt tttatgattt ttcatttttt cactcctgtt ctttttttga taaacaacca
    26221 gattgcaaag cttgcgaata ccataccaac aacgcctaaa agtgtatttt tcgtttcgtt
    26281 tgtttttgga agaaatggac ggctattttc ttttttattg actactttca atatactgtc
    26341 cgcctcttta tcctcagaaa gaaaagttga aatggtaaac ggaatttctg tttcacttaa
    26401 aatataacca gaaggtgctt cgatctcttt taatcgataa cttcctgttt tcaacccgga
    26461 aattgaaaaa gcgccatttt tatcggaaat caggtgaagc gcatctgatt cgttctttgt
    26521 ccacttgtat ccgttggctg tttgctggag gtaatttcct gctttgtttt taataaggaa
    26581 tactgcgcca gctaatcgtt tatctggttt atttgtctcg attttgacaa attgcttact
    26641 ccctgtatat acagagactt tctttgtact gacaccaaaa ccaaaatcca gtgttccgtt
    26701 gttgttgatt tccttgttcg cctgtgctgt actgcttagc tgcatctgat aagaaatggt
    26761 cagttttttt ccagcatatt tttccaacga agggatcgaa aataaaacat caaatccgtg
    26821 aggtttcttg tgtatgacaa agccttctgt cagcttttct ccattcgatg aaatcgttaa
    26881 actattaggt aaaaacgtta atgcttcatc cgcactatct gacaattcga acttttgata
    26941 gtcaaggata tttacaggaa ttgtcgtatg caaggaataa gtgatttttt caccaatcgt
    27001 aaaatcattt ctaggctctt ctaatacctt ctcaaaaggt ggatcagggt agtcgttttc
    27061 ttcattttta ggatacaaat ggatagttga aagtttttga ttgttttgtc catgtacagg
    27121 aagaacaact accatattct ctgccttttc tttgacgact tctggtactt tggattcaat
    27181 gaaaagatac gctttatctc ttttttcttg atctttagcg gccagagaaa aagaagcgat
    27241 accatcttcg ttgttaagag ttgtagttgt tgcttctgcg gtaaacatac cggaagacgc
    27301 accgtttttt gcgatctctg cttgtgcttc ttctaccgtt ttgccctttt cccgtagatg
    27361 gtaaaaagaa tctgtgacat cataaacttg gaatgtgaca ccatttaatc ctcgatacgt
    27421 ttgtaataaa gctttttctt gtccgtcatt tggatgattt ttcggcagtt gcccgtttgg
    27481 gaaaagcaat ttgtgtaaag taatctgaac catttctgtt tctgtttctg ttgctacctg
    27541 agtaaaagct ggtttaaata gtaacaagaa gagacacata ctaagccaac caagtttttt
    27601 catatttagc tgccccctct ttcttttttc aaaaaaagat accaaataga aaacgcacaa
    27661 ccaatcatcc ctactagata gattcctaaa cggccaattc ctcccgtttc aggtaatggt
    27721 acttttgcct gattcgtgat gtctaaagaa atctggttgt ttttggctcc tggtgacaga
    27781 acagattcat gatcttgtcc atccacttga attgacccat cttcgtgtat aactatagta
    27841 actggctctt ttagaccttg gtatccttct ggtgtaaaag tttcggtcag cgtataagtt
    27901 ccaggtttta aattttcgaa tagaaaggta tctgtttctt gtccatcttt tggcgattct
    27961 aagtccattt ctggtccctg cagtctgaat tttgctcctt ttaatgtctg tccgttgtct
    28021 tcttttttgt gtaccgttag atcaaacgct tttagtcgat tttgatgagt gagcgtccat
    28081 acattatttg tcatctcggt attttttccc gtgtaaatga tttttccata tttatcgatt
    28141 tggaattcat aatttcccgg cgaagtatca tatccggcag gaccagctgt ttcaacgact
    28201 cgatatttac caggtgtgag tgaatcaaaa ctggcaagac ctttttcatt cgttgtttgg
    28261 ctgtcaattg gctgataagt accatttgct tcttttttct gtagggaaaa tgctgctcct
    28321 ttaagattta tctctttgtc cgtcccttgc atcgcatact tacgtactgc tacaggaact
    28381 tcaacaaaag gattttcaat agtcaactgg atcgtatcat cggaagtagt gactgttttt
    28441 ccatcaatgg ttaccgtacc atcagaagcg atcttgattt cccaagtagt ctttttgctt
    28501 aatccatgcc cttctggagc ttttgtttct gtcagcgtat aggtcatttc tttttgcaat
    28561 ttgacatttt ctggaagaga ataggttccg tcgccatgat ctgttagtaa agtatcaata
    28621 gaatctcctg ttaatttgaa aacagcgcca ataagatcct tttcacctgt agaggaattt
    28681 ttcgttattt tcaagtttaa cggtgtgaat tgtttagtat ttttccatgt cattgcatct
    28741 atttgttgag aatcgtatcc tggtacaggt aattctttga ttgtttggta actgaatgct
    28801 tgaccttgat tattgtattg aggtagtgat aatatctctt gataactttc tccgctattt
    28861 gcagataatt tgtcaatgtc tgcacgttcc cacgtatttg ttgtatcttt agctggttta
    28921 atgattcgaa tatatccgtt tttccaagct gcagcatttg tcgtatgttc ccgttgaatc
    28981 tcaaaagtaa cttgatctgg acgatcagct agattgttgt caaattcttc ccataacttt
    29041 ttgatgtgaa gactgactcc gggagcttta gcagatggta taccgaattc agctaattca
    29101 ttggtatcaa tgtttggctg gaaagttgtc ctgccgttca tttgatacca gaaatttgga
    29161 tggaagtcct cattttctgt ttggattctc acttggtaat ggatttggat ttcttggtct
    29221 tttcctaaat agatctgatt gctcttgatg gtatttcctt ctatggaaat agatggagtg
    29281 accgttgtag gacttgtccc cacactcttg actgaaagtg taccaggctg ataaatgaat
    29341 ggttctgcga ttggatcatt tatttgtcca ttgcttacag ttgctgagat ctgtacagct
    29401 tttttggcta ggtattcaga gatatctgtt gcatgatcag ctgattcata gtaaagatcg
    29461 cctttttcat ctgatgaaac catttgtcgc atacgagact ctacttctgc ttttgagaga
    29521 ccagctgccg gatcgctttg aagttggatg ccaagaccat gtatttcgat tcctcgttct
    29581 ttgagtgcca tcgcttctcc gatggttgcg ataaacgtac tgtcgattct tctggataga
    29641 ttgttttggt caggtgcatc atagattctt gagattagag aagtatttcc cggccgatct
    29701 tgcgtattag aaaactgagt tccgtaataa ttgctgcttg attgtgcgtg tacccgctgt
    29761 actttatagg aaaatgttgg tacaccatcc gtcagcaaaa cgatcacttt tttatgtcca
    29821 tttggaacgg atagcatgct tcctgcatct cttagtgctt tttgtgtaaa agtaccacca
    29881 tttgtccgtg aaggtgtaat ggattttact tgatttttca ctgaatcaaa tgaacccatc
    29941 tgtactgcac cgttactgta gctatatcct tcgcttgagt agccgacata tcccatattg
    30001 attttgtctg tgataccgct atctgctaaa gtatcgacaa aacgatcgac accaatcttt
    30061 acttcaccga tccgattatt gtcgttcata cttcctgacc agtctacgac caatacgaga
    30121 tcaagaggcg tgatatcttt ttgtacattt ccacgagcat tcaaataaac atcaaacaat
    30181 ccgggtgtac ttgtttcctt tgcatacttt cgtatcgcat agtctgcttg attaccggtt
    30241 cctccatatt cgatatacga atgggtccga tcattaggat ctccattcca agaatttata
    30301 ccatcccatt gatttgttcc gtttttattg ccttgatgat caagaacatt tgtatttcct
    30361 gtaggttgcc aactggcttt aggataagtg ccctgatcat ctgttgtata ttctgtagga
    30421 atcgtttgat tagatggcac actcacagct ggcaaggata atgtaggcaa tgtaaatggg
    30481 ctgacagaat tttcgttttc ttttgatgat tcttcttcta tttcgtctgg ctttggtact
    30541 gacacatctg tatctttctt attttctgtc tcatcaataa tgtcatattc tttattatcg
    30601 acgatcagct tgtttgcctg gctgtggtaa agtgctggat cgattggaaa actcagatcg
    30661 atagtttgtt tagtgtttga tgagatttcc aacgtaattt tcccgctatt tacctgataa
    30721 gcatagccat ctccttcttt tctttccaca tcacttaagt aattttcgac tagaccaatt
    30781 tctttttcta tcttttcagc actggtgtta ttcgcattta gcttgatgtt cagtgctttt
    30841 tttccagact gatccgaaat gacttcagct gaaattgttt ctaaaactgt tggatcatct
    30901 gtagtgtctg ctattgcaga taatggcaca aagtttgata aactcaatat tatcagcatg
    30961 aaaataactt tcagtttctt ccctgtggtc gtcatttttc ccctcctttt tctttttttc
    31021 tgttttgacg tgaatagtga aaattattaa aataaacagg cgttacataa ttttatttat
    31081 tttaatatga taattattat acgaagggcg ctaaagggaa tcacaccaat tttcatacca
    31141 attggtttca tttatgaagt tgattccttc gattaatgag cgtttcattg aaagaaaagg
    31201 gttgagggag aaaaacatat gatcaggttg ttggaatgca actatagaaa gatggaaaaa
    31261 atcattcgaa ttctgtccat ttctgacaag tggatgacta aaaaagaact tgccaaacga
    31321 attggtagtt cagaatctac atttgtaagg tatatcgaag agatcaaaat ccgctgggga
    31381 acggatgtaa cgattcaatc ttcacaaaaa ttggggtatc gtcttgaaca tttgaatgta
    31441 ccagcttatt taaatgttct gatggaaatg tcgcaaagtt ctacaaacag tcgtttatta
    31501 aatgagatta ttgtcaatcc gggtaaaacg attgagtatt attgtgaaaa aatttccatc
    31561 agccggtctt cttttacacg taagttgaaa caatgcaatc aaattttaga aagctattta
    31621 ttaaaagtga tcgttgacca aggttttcag ttaactagcc ttaaaagtga gctccagttg
    31681 cgtatctttg ttaccttttt ctttttaagt tactacggaa gacgcacgtt gccatttcca
    31741 atcgaaaaaa agaagatcaa agaaattatg gtcaagaata attgtcagat gaatttgttt
    31801 caggaagaaa gcccatatga gcaatctttc tttattatgt attttgttgt gcatctcatg
    31861 agagaatccc aaggaactcc atttgaatgg aaagctggag actcttatgt acagttgata
    31921 aatatcgata aaaaagactt cttaatcttg aaaaatctct taggaaaatt gaagtacagc
    31981 agctatataa aagtagtgaa ctattttgtc aatacgattt tttcagaatt ttcggagaaa
    32041 gaaaaaaatg aaatcagtga gcagatacga aaaaatatgt cctactgttc gttttcatcc
    32101 gctgattgca aagaaaaaaa cgaagaatat gttgttcaat tattaacgca aatttatttt
    32161 ttctcccgta ttttcccata cgatacgact catttgacga tccgtgcaac ttactttgca
    32221 cagcagatgc aacaccgcca gatacgtcta tatgctggat ttcgtgcaga acttcaattt
    32281 tattccactc tactgaatca gaatctgatt tattatttat ctagctatat cttttggatg
    32341 cttatcggta atcctgaaat tcaccattat acttctgata aaaaaatact gattatcagc
    32401 gacttaagtc ttcgacatag tcaatatatt gaagaatata tcagtgatat tcttgctgtg
    32461 cataaaattc actctgagac aactgctatt actgaagatc agctgtcgaa atacaatctt
    32521 ctcgagtatg atctgatcgt tacgaatcaa cccattttga atgcccatgt tcctcatata
    32581 ttgattgatg atagtgtgtc attcgcaaat gaagaagaac tcatccgctt attcgagtta
    32641 tgaataatca aaaaacagct atgtataaac caccctgata aaaatttcag gacaaataca
    32701 catagctgtt tctttttatt gtattccttc agcaatccaa cgagatttat agcgaaagtc
    32761 ttttaacaac tcttcttttg tccaattatt tgcaatgaga tccgtcaaag tgttcattac
    32821 gagggtcatg gaaaagtata ggaaactaac tgcttcttcc gacgattgaa tggagaaatg
    32881 attgattctt aataattcca gccatttttc gttcattgga gaatcagccg atagttctct
    32941 acgtttggtt gtctgctgat catttcgctg ggttaacaag tataaccttt gccaatagat
    33001 actttcgtga tctaagtcgc agcaccactc taaatatttt tcgatcccca aaaagaaatg
    33061 ttgtttgttt tcgctaatga attttaagat atcttgatgg actatgtgag aactgctttt
    33121 gatcgtatac aaataagcat cttccaaatc ttgaaaatat ttataaaaag caccgcgcga
    33181 catccccatc ttgtcaacga tttctgagac tttgacttgg ctgatgtgct gtgagcaaaa
    33241 tatctccagt aataggtcat cgattctctg tttctttgcg ttcgataaat tgaaaaaagt
    33301 ttcttttggc atccacatct ctcctttatc tctgattata cgtggatagc ccttcattgt
    33361 catgaaggat tcattccttc tttttctctt cttctgtcaa gtacccatct tccattcgat
    33421 aaacgcgatc actccattga atcatacgga catcatgagt aaccataata gtcgcttttt
    33481 gtttctcatg agcttcttta actagtaatt tgacgacttc atatgcatga tctgtatcca
    33541 aactagctgt tggttcgtct gctaagatca agcttggctc attatataaa gcgcgagcaa
    33601 ttgccactct ctgtctttct ccaccagaaa ggtctttagg ataactattt ttaagtgact
    33661 ggatatctaa agattgcagc aaggaatcga tttttgttga atcttctttt tttgaagtaa
    33721 cttggtcaac tagttcaaat tgttccttta ccgttaaaaa aggaatcaaa ttagagcttt
    33781 ggagaataaa tcctatttct ttaaagcgta aatctgctcg ttttttttct ggaagctcag
    33841 ttagtgagtg accgttgatc actatttctc cggaagtcgg tgtttgcaat cctcctgaaa
    33901 tggtaaggaa agtactttta cctgaaccag aaggaccaat gatgctgacg aattctcctt
    33961 cctttacata aaaatcaatt gctttcaaag ctgagacctt tgtatgacca gaaccaaatg
    34021 ttttggtaac ttttttcatc gttaatattt ttttcatttt tattaacctc ctattgctgt
    34081 aattgggtct actttggtta tcgtacgtat cgaaaacaat ccgcctagca ttgcgacaat
    34141 gatcaaaatc ccactatata gcaaccattg tgaccaaaaa acagcaaaag gcatcgcgct
    34201 tggtaaaatc agactagtca aataagcaaa caaaagagca agcagaacgc ctaccacccc
    34261 aacaataaag gactgtgcta ctaaagattt agcgatgaag aaatttttga tcccttgtgc
    34321 tttcattact ccaaaaatag cagtcttctg aagtgtcatc acgtacatga aaataccgac
    34381 cacggcagca accactaaga acaaaaagta tatcatcgca tttaaggtca tattttgggc
    34441 agaatagcca ggaatacttt caatgaatgt cccaatagtt aataactgta gacccttcga
    34501 tgctttttct ttcgaaattt tagctgaatc ctttattacg atcccattga tcggctgttc
    34561 ttcttcagaa gcaaacggct gttgtccata cttcaaagca gtccacgtat ctagggacgc
    34621 atagatgacg ggagaaactg tgtaataagt ttcaggaaag ataccgacaa tcgtcaaagg
    34681 atcgtcgtaa ctgccaattt tgatcgtatc tccaatttta tagcctgaat cagccagatt
    34741 ttgagagatg atgatttcat tctttgtttt aaattcatgc cctttcgtga gttttggtaa
    34801 taaaaatgct tctttcgttg tgccgaaaac tgtaacattt tctttgtttt ttcctttgat
    34861 tgctccgctg tacaatccga ttggtgcttt ctctcctcct tcaatgtatt tcagatctcc
    34921 tcttgttaat tgtgaagctg caaatacttg atttgcatcc tccgacaaaa caatctcttg
    34981 tgcgtcccaa tcatcgattg cttttttaaa ctcttctgaa agtccattcg ctagtcctga
    35041 caacatgaat acgacatacg caatcaagaa cataataccg acgatcaagc catatcgtaa
    35101 tttagaatag cgaatttctt tgatagctag aaacatttgt ttttcctccc tatgatagat
    35161 tgatagtgac aaaatgtcac ttgttgaata gtgtaactct aaagtgacaa tttgtcactt
    35221 gtgttgtttt tttcttatca ataaattgtc taagacatct ttcgataaaa ccttaacatg
    35281 agtcataaaa aatttattta agccttattt atgctttcca tgataaacta aagtgaccaa
    35341 atcaatgagg tgggaaaatg attaaactat tagtagtaga agacgataaa gtattatcca
    35401 ataatataaa agaaatactc gctgcaatcg gcgagataac acaagtttat gatggtagtg
    35461 aaggactgtt cgagttggaa agtggagttt atgatcttgc aatcttggat ttgatgatgc
    35521 cggaaatgaa tggatatgaa gtgttagcaa aaatacggaa agaaaaaata cagactccag
    35581 tattgatttt gactgcaaaa gatagtttgg aagataagat tgaaggattt gaacgaggtg
    35641 cagatgatta tctaacaaaa cctttctatc gagaagaact gatcatgcgc gtgaaagcat
    35701 tattgaaacg ctcgctaggt ctctttgatg aaaatgttct gaagtataag gatatcagtt
    35761 gtgatttaaa aagcaatgaa gtcttgtata aagaacaagt tttgcccata caaggaaaag
    35821 agtttgaatt gctcgtttat tttttacaaa ataagggaat cattttgaca aaagagcaga
    35881 tatttgaccg gatctgggga tttgattctg atacgacgat tacagttatt gaagtttata
    35941 tgagcaactt acgaaaacat ctgcgaagta ctggattgga taaagaaatc aaaaccttac
    36001 gtaatgtcgg ttatatactg caaagggtgt gatgcaatga atggagatat tacacgaaag
    36061 caacgtagtc gcttttttgc gacaaatgta gcagcatttg cttttatctt ttttcttttg
    36121 ggattcatta ctttacagat attaaaccaa tctgcataca gagaaacaga tctgtcatta
    36181 gagaaaataa attttgattc tcgaacagta caattagaga tacaaagata tcagcaaggc
    36241 gatccctttt taaacccgac tcaaacggaa caaaacagaa caaacatgga acagccatta
    36301 atggaaccat caaatgaccg ttttaacact caagtgatct tgtggtctaa aaatggtgag
    36361 atattgaata aagcagctat tggtgggcgt ttcaaccaaa tcaaagaatt aaaaatggat
    36421 acgaaaaatc tgaataccat ccaaaatatt gaattaaatg aagacagtgg ctcatctgcc
    36481 ctctctttcc gttcgattac gaaaaaagcg gagacgaatc aagatggcgt tgcctacatc
    36541 caaattttag aaaatacaaa tcaaatcaag aattcattag cgacttttcg cacgattatg
    36601 attttgtgta tgattgtttt ttggattctc tctattggta tcagttatta cttgtctagt
    36661 ctaagcatgc gccctattat tctttcttgg agaagacaaa aagaatttgt tgagaatgct
    36721 tctcatgaat tgcgcacacc gttaacgatt attcaaaata gtttggaaca tctttttacc
    36781 aagcctaatc atactattat tgaagaatcc gaaagcatcg ctcaagcatt gtcagagacg
    36841 aggcgcctca ctggattgac ttcggatttg ctgacaatcg ctcggaatga ttctgatcag
    36901 cagctgcttt caaagcaaat gatcaataca caagaatata tcgaacaact tgttaaacca
    36961 tttcaagaga tggccatcat agatggaaaa gagtttattc tccataacga tgcaacatta
    37021 gaagtaccaa ttgatgaaaa aaaaatccat caagttttgg ttattttgct ggataatgca
    37081 ttaaaatata cgaatccaga agataagatc attttggatt catctgttaa ccataaagaa
    37141 tggctgattc acgtaaagaa tactggccct tctatcagtg aagaagatca agcaaggatt
    37201 tttgatcgat tttatcggga agatcaatct cgggcgaaag agactggcgg atacgggctt
    37261 ggcttggcaa ttgcaaaaca ggtaatcgaa cagcatcatg gacagatttc tgtatccaat
    37321 tatcttccac aaggagtcga ttttaaagtc caattgccgt tgaagcctaa aaaataatgc
    37381 ttatccctaa aaaagggctg tgacaaaaat cttgtcacag cccttttttg ttgcgaataa
    37441 acggtgttcc agaagtagct tcttcggaaa taagccgaaa tttctttaag ctgaccactt
    37501 ctgtcacaac ctctttagct tacgcttacg attttttatt gcggtcttca atgtgtacat
    37561 caccgcttgt gtcaatatcc aattcttctc ggctaacatc ttctgtcact ttttcaacat
    37621 cttcatgttc ttctttatga atcccaactt cttcacgaat aactgtatgt ttgcttacat
    37681 ctacttgctc ttctttgatt gggatagtaa tcgtgtcatc ttccaatttt tcgtctgttc
    37741 cctgtgaaga ttgatctgtt actggtttgc gttcgatgac gatttcttca cgtttgaccg
    37801 gtacttctac cgtttcagta tcgttgacca catgtttgtg gatgtcgact tctccagttg
    37861 tcacatcatg tgtatttaca tctaattttt cttcttttag ttgaattgta tcatcatcta
    37921 gatttggttt gcgatcgatg tcttcagtct tgcttgtacc cattgtatca gtctcgccag
    37981 gtacgtccat cggatcagtt cctggtgtca tattcaatgg atccgttgta ttttgaaagc
    38041 ctgtcgtgcc acctacgtcc attggatctg ttccagtgcc agtttctaat ccagtcccca
    38101 ttgtgcttgc tgggctaaca gtatcttgtg tatccattgt atcttctgtt gtttctggct
    38161 gacgatagcc ttttactgtg atgacaaatt ttccatcaga aatatctcct ctatattgat
    38221 ataaaggatc attttctggt gtagccgtct cagaatcata ggtatcaaaa gaaaaagtat
    38281 cttttatttt ttcccataaa gaacggtctt cttcaccatc catccgattt tcatctgttt
    38341 caacatctat accagacaat ccgtcaaatc ctagtctaga tgctgcttca tcagtagtat
    38401 aaataacgat atcatctcgt tcataccctt cgttacgcaa ccgttcaatt actgataatg
    38461 cttcttccct cgtactatag cttccgatga ccgttaaatt tttctcttcc atgtaaattc
    38521 ctcctagcaa atagatttat gattggtata ttgttactta taaatttatt ctataggaga
    38581 cgattgtttt ctacaaatat ttagattgga atttgattaa aacaatttaa ttgaaatttg
    38641 aaaacattta tgttaatgaa agtcatattc taataaaatg tcatttgttt ctatatgaat
    38701 ttttatgaaa aatttcatat aatttccact cgttgtgatg gtttaattta tccaatccat
    38761 caataagcaa acaacttata taaaaaagga ttaattctca atataattgc tcctaccata
    38821 gaataaatct tcatgtaaag taatgaagta ccaactattt ctaatacttc tttaagaatt
    38881 atcccaacaa tatcgttaga caaaccatta ttgaataaat ggagagccaa tcgattctga
    38941 aaaaatgttt tctaattttt cggaaataaa agaaagagcc tcctgccatt agaacgaacc
    39001 aaatccattt cattgctcat gaagtaacca catagctaaa aagaggataa atcaattaaa
    39061 agatcaaaag aatgactgca ctaataaaat aactaaaaat gtatcttctt acttcatatc
    39121 ttttagctag tacttagtta aaaatttgct tcctaagatc aagacaaact gtctatcttc
    39181 atttgatgaa atgcggtaaa atatagaaaa tgaacactat tggagggaat ttgtaatgga
    39241 cagcaaaact agaaaacaac tgcaagaaaa gttaaagcaa cgtacacact tgctcccttc
    39301 cgtctttcaa cagtttttag aagaaaatcg ttcttccctt tcattaggca gtcgctatga
    39361 atacgcaaaa gattttcatc tgtttactga ttatttgaga aaagtatttt ctgaagaagt
    39421 ggatgatcag ctgattatcc atttagaaaa acaagattat cagaatttct tagctaaaat
    39481 ctatcgacat actcgttcgt tccaaactac agcgaaccaa gaaacggagc aattgtttga
    39541 aaatagctat tacgggattt caaggaagca atattcatta aatcattttt tacgattttt
    39601 gtatcaaaaa ggactgattg aagaagagat ttctcttttg ggtacaagtt taccggaaac
    39661 aaccaaagca gctccgcgat atatcgatgc agaacttttc aaagattttg agcatttgtt
    39721 tatcccgatt gaaggctttc aaacaagcag agaagctagc tttgaagaaa agaatagacc
    39781 gaggaacctt gcaattactg ccattcttct gtattgcgga ttaaagatcc atgaagtagt
    39841 agaacttaaa cgtaaagatg tatctttgac caagcagatt cttaatctga atagaaaaga
    39901 aaacagactg aataaagtac caataccaaa agaagcgatg ccatttctgg agaactattt
    39961 taagttaaca gcacaaaata cggacgaaaa ttcttttgtt tttcgttcct cacatgatca
    40021 gcaaatttct cctcggacta ttcgccaaat tttgagcaaa attacagtat ataaaaacgt
    40081 atcgccagaa ctatgcagaa aaacatatcg ttattatgca gaactatatt tagatgatgc
    40141 tgacgcggta aattatttat gtggtaatcg atacctctca tcccattctt ttcaagatat
    40201 ctgggaaaag atggttgatt tttcttatca cgaattagcg gcattcgttc gggtttcttc
    40261 catataaaac aaatgtatga tctttttttc acaataaact atgttattat ataaaggatt
    40321 atgttacgat tatcttgaat ttgtatatta cttaacaaaa gataatggag tgtgaatgat
    40381 ggagaccata acgtattccc ctttgaactt atttacgaat tttcaagaag ctgcagctaa
    40441 cttttccgag accccgatta tctttgatga gccactttca gctttcccag aattaggact
    40501 tgaaacaact tacaaaaaat gctgcgaaag tgtgttgaaa cgggcttatc aattggctca
    40561 tcttggagtg aaatccggag acaaagttat tatttataag agttccgctt ttgacacata
    40621 ccttctagct gtttctgtgt cttaccttgg tgcagtgcct gtcatgactt cttatcatct
    40681 accaacaaca acaatggaag tatttatcga tcgtttagaa gaccctttca ttttatttga
    40741 tgatgagaca aaaaagcgag tgagagaaat ttcaaatgga acaaaatcaa aacagattcc
    40801 tattctttat ttaatggaac aacctgcttt gtctgtttct caatcatttt tagacaaaaa
    40861 tgaaatttct tatatgaccc atacttctgg tacaactggt atccctaaac ttatttgtca
    40921 ttctgctcat tctatgggat ggcggacgaa atggcaaaaa acagttttca caaaaatttc
    40981 ggaaaagaaa ttaatcgctt ttcacatctc tccagtgcat tccagattca atattggtat
    41041 ctcttctttg atgtccatgg gatttcctat gatgccttta gcaaacgcac aaagttcaaa
    41101 agttgttcat atgttggaag ctcaccggcc aattgcacta gagacccatc cgaataactt
    41161 tgttcagtgg cgttttactg ctaaggagca tccagaagct ttttcgggta tccgatacta
    41221 tcactccact tttgatgcta taaacaacca gacaatgaag acatttttgc gaacgtcgat
    41281 ggcacaggat gcggtatttc ttcaagtata tggccaaagt gaatgcggac caatgattct
    41341 taaggctcat acactagaga gtttgaaaac aagtgatgca agagatatgg gcgttggact
    41401 ggaagacatg acaagcgctc ggattacaga ttcagttggg aatgtcctac ctgaaaacac
    41461 agatggtcat atacaattac tgtctaaagg acgggctttg acttattaca aggaagatgc
    41521 acggttccag gaaaatgttt atggcgattg gtgggatagc ggagattacg gttttatgga
    41581 taagcatggc cacctatttt tgaaagatcg gcaagtggat ttgatcgaaa cgatcaatag
    41641 tacattagct attgaagact ttttattaga ttcgttagat tttttggcag aagtagtgat
    41701 tgtaagggat aaaaatcatt ctccacaacc gattattgct ttagctcctg ataaagaaat
    41761 ggattggaat cgatggtggg agcaagtcca tgaacttcct cgcttgaacg tccctatcat
    41821 ccgtgatttt gatgcaatcc cgcgaacagc tacaatgaaa gtgcaacggc ttcaaatcga
    41881 gaaagaatta aaatcacaaa agtcctaact atttaaggaa agaaagatcc attttggtct
    41941 ttcttttctt tttgtttttt taaattggat gagataaaaa tatgccaatt ggctgttttt
    42001 cttttttgaa aatttcgtta tactcttatt catggaggaa aatgttatga aaagaatctt
    42061 gacaattggc ggttctgatc catttgctgg tggaggaatc caaacagatt taaaaacttt
    42121 tgagaatttt ggtctattcg gactaagcgc cttgacttct ataggggcac tagatattaa
    42181 tgagcgattt atgctggaat ctatatcttt ggaattgctt aaacgacagt tagtttcgat
    42241 tgataaaatg acttcattag atggtatcaa aattgggtta ttgaattcaa gtgaagcaat
    42301 catggttgtt cgagacttca taaaaacaaa acaaaatatc ccaatcattt tagaccctgt
    42361 tttggcattc aaagaaactg aagctgtgat caatcaattg tatatttcgc agctcatcga
    42421 agaacttttt ccacttgtta cgatgattac gcctaatttg gcagaagctg ctttactttc
    42481 tggtcaagct cttccctctt cgttaacaga aatgatccat ctcgctaaag aactcactgc
    42541 ttcaggaaca aaaaacattg tgatcaaagg cggcacaagg atggctggag atgaagctgt
    42601 ggatcttctc tattcaattg atcattatga ggtattttct cataaaaaaa tcgacactac
    42661 aacagtaaac ggggccggtt gtgccttttc ttctgcaata acagcgaatc ttgtgttagg
    42721 ggcagatttt accaaagcag tagcagaaag taaaaaattt gttgcccatt gtatttctaa
    42781 tggcgtgctg atgaacgatc ataccggaag tgtctggtct ggcggtattc aaaaaggagg
    42841 aatgagaaat gaaaactaag caattaacga tctatgctat gctgattgcg ttgacagttg
    42901 ccctttcatt aacaattttg atccctgtac cggctacgaa tggatttgtg acattatgcg
    42961 aggccgggat ctataccgct gcttttcttt atggtccaac gggtggatta gttgttggtg
    43021 cttccagtgg tctattgatc gatttgattt caggatatcc gcagtgggca gttttttcat
    43081 tagtgatcca cgggttacaa ggatatgttg ctggttcttt cagcaaatct tctactcgta
    43141 tgtggggatt agggctttta ttaggaagct tagttatgat catcggctat ggtatcgccg
    43201 gttggatttt atatgattgg ccggctggag tggcttctat accaggaaac atcttccaaa
    43261 atctgattgg aatgggactt gcctttccta taactgcaag tcttcaacgt attcaagcac
    43321 ggaagcaata ttaattgaaa aagaaggttt tagaaaatga agttgtcaga aaaagaatta
    43381 aaagaccaat tgactgaaat agttaatgac atattggctg aggcacactt gaaaaaaggt
    43441 gatctttttg ttttaggatg cagcacaagc gaagtcgttg gaggacatat tggaaaaaat
    43501 tccagtgcgg aagttggtca atggatcatc cgtacgttaa aagagttgtt ggatcctaaa
    43561 gaaattgcgc tagctgtcca aggttgtgaa catttgaacc gagcattagt agtagaacga
    43621 acagtggcag aagcaaaaaa tttcgaaatt gtctctgttg ttcctgcact acatgctgga
    43681 ggtgcttgtt ctgttgctgc ttttgaccag ttcaatgatc ctgtcgaagt ggaacacgta
    43741 gttggacaag ccggtatcga tatcggtgac acatcaatcg gtatgcatat caagcatgtc
    43801 caagtacctg tcagacctcg tttgaaaacc cttggacaag ctcacgtgac agcccttcgc
    43861 tctcgtccta aatatatcgg tggtcctaga gcgaattatt gaaatgagta attgacagag
    43921 caatctgaaa aacttcctcg caaagtccaa ctaatgaact ttaacgagga agttttactt
    43981 gtttgtttta tataaccgag cttcataagg tgctagtatg cctttagctt caaattctcc
    44041 gctagaggaa tttgacaaga gtaatgtcca ggactgatct gcttcttttg gaagttcata
    44101 gtcagcaagt gattctgtta agtttaccat aataagatat tgttcatctt ctaaatatct
    44161 ttcatagaca taaagttgtg gatgttcatg aagatattct ttataagaac cgtatatcag
    44221 acctttattg gtttgtctaa tgcggatcat gttcttatag aattgcagga ccgactctgg
    44281 ttcatattct tgttgttcta cattgatcgt tttggtattt ggattagtga tcagccaagg
    44341 ttcatgtgta gaaaaaccag catattgttc cgaagtccat tgcatgggtg tcctgctgtt
    44401 gtctctcgtt gtttcacgaa tgatatccaa tgcttcttct cttgtcttac cttccgcaag
    44461 cagcctttta taaagtcttt tcgaatctac tgcatcaact tgatcgatcg attcaaaggg
    44521 catattcgtc attccgattt cctgtccttg ataaataaat ggcgtacctt gcaaaaagaa
    44581 atacatcata gcgattgctt ttgcagacat agcccaaaaa tcgggacgag tatctccaaa
    44641 cactgaaacg gcacgaggaa catcatggtt ttccatatat aaagcgttcc atccgatacc
    44701 atccaatgaa gtttgccaag cgcttaatgc atgtttcaat ttaaggacat ctaattgccc
    44761 ttctttttct tgctgccaga tatggatatg atcaaattcg aatatcatat cgaaataacc
    44821 gtctttccct acccattgtg gtccttcttc tgcagtgaca ccacttgctt ctccgacagt
    44881 catgatatcg tactcttcaa aaacatgttt caaatcggtc aaatgttctt cgattcctga
    44941 gacattttga aagggtgaga aagggttctc tgtggctttt actgaatatt catctttttt
    45001 tacatgagaa atcgcatcta aacgaaatcc atctatccct ttatccaacc accatcggat
    45061 catattgaat agttcttctt tcaatttctc gctttcccaa tttaagtctg gttgttcttt
    45121 tgcaaaaaca tgcagataat actgtccagt ttcatgagaa tactcccaag cagaaccgcc
    45181 gaaaatcgat gtccaattat tcggttcttt gccatctacc ccgtcgaccc aaatgtaata
    45241 atcgcgatac gggctttctt tagatttttt cgattcttca aaccattgat gctgatccga
    45301 actatggttg atgacaagat ccataatcac tttgatccct aattcatgtg cttcttttag
    45361 caataaatca aaatcttcca tcgttccgaa tctctcttct atatcttgat agtcacttac
    45421 gtcataacca ttatcgacaa atggagattg atagatcgga ttgatccaga taaagccgat
    45481 accaagatct ttcaaataat taagtttttc acggatccca ttgatgtctc cttgaccatc
    45541 gccattgcta tctttgaaac tagcaggata gatttgatag cctactgctt ctttccacca
    45601 tttttgttct tgtatcatat cgtttcactc ctttaacggt ccctcttgat tataaccgat
    45661 tctattttag aagtatcctt gttaccggta actagaaacg aaaagctcaa cgaatctgct
    45721 aagtgcgttc gttgagcttt attttatcat agatagcggg caatcagttg aatcatttgc
    45781 ttgggaaatt cattcaagtc agtaatatct acaaaacgac tttcaccgta gatcgacttg
    45841 atttcttctt tgtcttggcc aatagctgcg gagacaaata ggattccttg acgttcatat
    45901 tctttcaaaa cagactggat atcttctttt gcttttttgc ctgtataatc cggcaatgct
    45961 tttggctgac cgtcactaat attaagcaac agtttggttg ttgcggattg tctgtttaat
    46021 ttttctgctg ctaaacgaag tgcagcacca tcacgattgt tttgccttgg tttcatagtg
    46081 actaattttt catttaacca ttgaaatccg tcttcaaatt ctttataaga aaacaaagaa
    46141 gtcttttctc ttgacgaacg gtctgctgta tctccgtaaa tcaacaatgg gatatcgact
    46201 ttcttagaaa attcggcaat ggcaatcgct gctttttttg ccgcttcgat ccgatcatcc
    46261 cgtatcattg acccagattc atcgatccgt acagctaccg ctaaagaagg ttgctcatgt
    46321 ggcgggttct ttttgtcaaa atttctcaaa tcaccatatg ctactttgct tgcattgaac
    46381 cgctgacctt cgtattttcc tttttggtac gtctctgttt gttcattatc cagtaactct
    46441 aaaatttgcc ggctaagtga ttctactatt ggcattactt gcatgtgtag ctcacgtgct
    46501 tccggcgttt ttccttggaa agctggtcgg tgaacgatga gaccttcttt ttcatgaatc
    46561 gtttccttca tgatttgtcg cgcttcttta ttcagttgct tcttgatttc cttatcatgt
    46621 gcttccatgt cagaagcaga catttcttga gaatcatctc ctgtttgttg ttctttttga
    46681 ttttttcctt cgatatcgct actgcgttct ttttgcattt gaggaggaat ctcgtttttt
    46741 tctattgttt cttcttgttt ctcttctccc tcatttggtt cttcagcaga caacgatttc
    46801 ttttcatcta agcgcagaat ttcttcagca ttttcagtga tgtttcgttc atttgcttct
    46861 tcggcgctaa tggttctttc ttttgtatgc tcagattgag gatgacccca tttttctttt
    46921 ctcaatatgt cctgaagttc cagtagcaat ccactgtcag acaaagcttg ttcaagaatt
    46981 gccaattcat ctgaatcagt ggataattta tacaaaactt ttggataaat cgactggagt
    47041 aaatcttgtc cttgcttata ggtatttgcc caataaaaat aagaacgcat tcctgctaca
    47101 cctttgatgg cgttagcttt tgcagtttca tccaacaaac ggataatctc tgccatcttt
    47161 aagagaagtt cttgtttttg gatcatagtt ttcgccatcg cacgttctgc catgacttct
    47221 aaggcaggaa gatccatttt ttccgcatgt tgcatccgat ctcttaaaga ttcatttaat
    47281 gggcgatttc cttggtaatt tcgatttgta gtgatcacaa tgacgcaatc tggatggcga
    47341 cgaatgattc ctgtcggtaa attcaacatc ccatttggtt cgagcgctga gttaagtgcc
    47401 acaagaacag aggcatctcg gatgacagtc ggctcttgaa tctctaataa atagcctttt
    47461 tcaatcgctc gaacgatttc agaagggtaa tagctgtaat ggattgcatc tggataatca
    47521 gcttcgatcc tttgaactag ctctgctaat ttttctttag cagaaaaagg atcaatgccg
    47581 taatggttct ggatcagtgt cagtacagct tcaagtgtat ctgtttggta aatagcttct
    47641 agtaattctt ggtcttgttc cttattagaa tctactacag gtaataaggc tccgaaaaca
    47701 tctgatttgt ccatatctgc aaaacaggtc acttttgtat agggaagttg cagatcagca
    47761 gatagtgctt tggctaattg tgtcttcccc gacccggcat ctccttccaa aagtacgtta
    47821 ctgattttca tttcaggatc ttgccaattc cctttgattt cggaggcgat ccgccattct
    47881 tctgcacttg tccgatggct tttcggtttc ttccagatca tttgcttttc tagccgtgaa
    47941 aaggttcggt tttctgttag tgagaaattc tccatgcttc tcttcccctt ttgatcatct
    48001 ctgtctatta atatcctagc tcatctaaaa gacgttttaa aatacggaat cgttccccaa
    48061 tcagttctcc gtctttttgt agtgtatcgt aatacaaaat gatgtcttca tcgatctttg
    48121 gatttttggt atctacttct accgtcattc ggttcccttg taatccaatg atgtctacga
    48181 ctggattttc tggatcataa aatttttcat aacgataagc atcttggaag tataaacttc
    48241 cttgcgcagc taagatcgct aaatctaaaa tacgtgtgat tggtaattct tccgactgtc
    48301 gtgaccattt ttctccagta tgtcgccata ccttgcctga gatatcgact tttccacgat
    48361 cattccactg agcaaggccc agtgaaagcc cttttgcatc catatttaat gcatttcttc
    48421 catcaacttt atcatagtct tctacaacga ttactggttt atgttttaaa tttgttggta
    48481 tttccattat tttaactcct catcctttta tttttagtaa ataagtaaat tagtagttta
    48541 ctcatttact aaactatatg cgacattata gaggattgaa gtgaggattg caaagaaaaa
    48601 gagcttcatt gatagaaata aaaacaccat ctcgttcttt tacacgctca ttagtaacat
    48661 gtagaaagtc caagatgatg ttggaatatt ttggctctat actttttgat gttggtgaac
    48721 ttcaccaaca tcttttttc
//
LOCUS       DACDYJ010000017        45749 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDYJ010000017 DACDYJ010000000
VERSION     DACDYJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.017"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            101..544
                     /locus_tag="IWM51_000925"
     CDS             101..544
                     /locus_tag="IWM51_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9970361.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(550..1734)
                     /locus_tag="IWM51_000926"
     CDS             complement(550..1734)
                     /locus_tag="IWM51_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP9970362.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2002..2700
                     /locus_tag="IWM51_000927"
     CDS             2002..2700
                     /locus_tag="IWM51_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP9970363.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2707..3384
                     /gene="nth"
                     /locus_tag="IWM51_000928"
     CDS             2707..3384
                     /gene="nth"
                     /locus_tag="IWM51_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP9970364.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3510..4043)
                     /locus_tag="IWM51_000929"
     CDS             complement(3510..4043)
                     /locus_tag="IWM51_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970365.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4340..6721)
                     /locus_tag="IWM51_000930"
     CDS             complement(4340..6721)
                     /locus_tag="IWM51_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9970366.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6711..7358)
                     /gene="recU"
                     /locus_tag="IWM51_000931"
     CDS             complement(6711..7358)
                     /gene="recU"
                     /locus_tag="IWM51_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP9970367.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7422..7961
                     /locus_tag="IWM51_000932"
     CDS             7422..7961
                     /locus_tag="IWM51_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 family protein"
                     /protein_id="HAP9970368.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISIDDLQNFSE"
     gene            8051..8470
                     /gene="gpsB"
                     /locus_tag="IWM51_000933"
     CDS             8051..8470
                     /gene="gpsB"
                     /locus_tag="IWM51_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP9970369.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8492..8859
                     /gene="rnpB"
                     /locus_tag="IWM51_000934"
     ncRNA           8492..8859
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWM51_000934"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9068..10234
                     /locus_tag="IWM51_000935"
     CDS             9068..10234
                     /locus_tag="IWM51_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP9970370.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10280..11776
                     /locus_tag="IWM51_000936"
     CDS             10280..11776
                     /locus_tag="IWM51_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP9970371.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11857..12540
                     /locus_tag="IWM51_000937"
     CDS             11857..12540
                     /locus_tag="IWM51_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9970372.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            13185..14375
                     /locus_tag="IWM51_000938"
     CDS             13185..14375
                     /locus_tag="IWM51_000938"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP9970373.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            14477..15430
                     /locus_tag="IWM51_000939"
     CDS             14477..15430
                     /locus_tag="IWM51_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP9970374.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            15499..16848
                     /locus_tag="IWM51_000940"
     CDS             15499..16848
                     /locus_tag="IWM51_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9970375.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVIAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(17031..17354)
                     /locus_tag="IWM51_000941"
     CDS             complement(17031..17354)
                     /locus_tag="IWM51_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970376.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            17483..18646
                     /locus_tag="IWM51_000942"
     CDS             17483..18646
                     /locus_tag="IWM51_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970377.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            18716..20065
                     /locus_tag="IWM51_000943"
     CDS             18716..20065
                     /locus_tag="IWM51_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9970378.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(20110..20541)
                     /locus_tag="IWM51_000944"
     CDS             complement(20110..20541)
                     /locus_tag="IWM51_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9970379.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEIDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            20929..23571
                     /gene="alaS"
                     /locus_tag="IWM51_000945"
     CDS             20929..23571
                     /gene="alaS"
                     /locus_tag="IWM51_000945"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP9970380.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            23771..24367
                     /locus_tag="IWM51_000946"
     CDS             23771..24367
                     /locus_tag="IWM51_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9970381.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            24489..25637
                     /locus_tag="IWM51_000947"
     CDS             24489..25637
                     /locus_tag="IWM51_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9970382.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(25675..26088)
                     /locus_tag="IWM51_000948"
     CDS             complement(25675..26088)
                     /locus_tag="IWM51_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP9970383.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            26167..26598
                     /locus_tag="IWM51_000949"
     CDS             26167..26598
                     /locus_tag="IWM51_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP9970384.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(26697..26930)
                     /locus_tag="IWM51_000950"
     CDS             complement(26697..26930)
                     /locus_tag="IWM51_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP9970385.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            27113..28783
                     /locus_tag="IWM51_000951"
     CDS             27113..28783
                     /locus_tag="IWM51_000951"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP9970386.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            28930..29316
                     /locus_tag="IWM51_000952"
     CDS             28930..29316
                     /locus_tag="IWM51_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970387.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            29303..30001
                     /locus_tag="IWM51_000953"
     CDS             29303..30001
                     /locus_tag="IWM51_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9970388.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            30006..30470
                     /locus_tag="IWM51_000954"
     CDS             30006..30470
                     /locus_tag="IWM51_000954"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP9970389.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            30467..31378
                     /locus_tag="IWM51_000955"
     CDS             30467..31378
                     /locus_tag="IWM51_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9970390.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            31613..32152
                     /gene="pyrR"
                     /locus_tag="IWM51_000956"
     CDS             31613..32152
                     /gene="pyrR"
                     /locus_tag="IWM51_000956"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP9970391.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            32166..33449
                     /locus_tag="IWM51_000957"
     CDS             32166..33449
                     /locus_tag="IWM51_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP9970392.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            33462..34388
                     /locus_tag="IWM51_000958"
     CDS             33462..34388
                     /locus_tag="IWM51_000958"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP9970393.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            34407..35687
                     /locus_tag="IWM51_000959"
     CDS             34407..35687
                     /locus_tag="IWM51_000959"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP9970394.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            35692..36768
                     /gene="carA"
                     /locus_tag="IWM51_000960"
     CDS             35692..36768
                     /gene="carA"
                     /locus_tag="IWM51_000960"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP9970395.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            36773..39958
                     /gene="carB"
                     /locus_tag="IWM51_000961"
     CDS             36773..39958
                     /gene="carB"
                     /locus_tag="IWM51_000961"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP9970396.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            40012..40788
                     /locus_tag="IWM51_000962"
     CDS             40012..40788
                     /locus_tag="IWM51_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP9970397.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            40785..41717
                     /locus_tag="IWM51_000963"
     CDS             40785..41717
                     /locus_tag="IWM51_000963"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP9970398.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            41714..42430
                     /gene="pyrF"
                     /locus_tag="IWM51_000964"
     CDS             41714..42430
                     /gene="pyrF"
                     /locus_tag="IWM51_000964"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP9970399.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            42437..43066
                     /locus_tag="IWM51_000965"
     CDS             42437..43066
                     /locus_tag="IWM51_000965"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP9970400.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            43054..43368
                     /locus_tag="IWM51_000966"
     CDS             43054..43368
                     /locus_tag="IWM51_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970401.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            43406..44047
                     /locus_tag="IWM51_000967"
     CDS             43406..44047
                     /locus_tag="IWM51_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9970402.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            44078..44782
                     /locus_tag="IWM51_000968"
     CDS             44078..44782
                     /locus_tag="IWM51_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP9970403.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            44779..45681
                     /locus_tag="IWM51_000969"
     CDS             44779..45681
                     /locus_tag="IWM51_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9970404.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
BASE COUNT        15004 a         7997 c         9882 g        12866 t
ORIGIN      
        1 tttttcttgg tttcagattg ggttttccta caaaatattt ttatctttgt tgaataggaa
       61 acaaaaaaac aatataatga aagaaaaaga aggtgtagaa atggataaaa gacaactgct
      121 aatagaagaa tatctggatt tagctaatga gattattcat cgtaataaac cagaaatgga
      181 aaaagaattt gcaggattta ctctaaatga actagaagtg attgaacata tcgggaaaat
      241 ccctgatcct aacgtgacaa aattagcgaa tgctagttac atgactagag gagccatcag
      301 taagctaaca aaaaaactga tagctagagg gatcataaac acttatcaaa gcgaagagaa
      361 taaaaaagaa atctattttc aattgacgcc aatcggacaa aaaataagga atagacacga
      421 gcaactccat caaaaatggg tacagcgaga tgctactgta tttgaaaata tgtcaaaaga
      481 agaatttgac acagtatttc gttttatcgg tcgatacaga gaatttatac gaagtagcaa
      541 atgaagctac tattttttat tgtagtgata ataggcaaaa atcataagca aaaatccgaa
      601 taccgtcgga agaaatggtg ctgcagcata aagctgtcct gcgattagcg ggccgatgac
      661 gcgcgataga gattgtatac tttgggtacc gcccattaca agaccttgct ggttatctga
      721 gacagaattg gatagttggc cattgaatac aggagtaaaa atagaatcac cgaaaccata
      781 acagataatc ccaaataaaa acaaaatcgg cagtagaaga attccagata atgtgatgaa
      841 tagataggcg aggatttcac tgaacatccc tattcttgtg atctgctgtt ctgaaaattt
      901 tttgagcaat cgaggcataa tgaacaattg cgagacagta tccaagattc caatcaaaga
      961 gaagcttaaa ccaaccaacc cagccttcca ctgaaaggcg tcaatcgaaa attgagaaaa
     1021 gatagattga agagagccag ctgccaacca aacagcaaaa cctgctgtca ataaaggaat
     1081 aacggatgaa atcttgaata attgttgtaa ttggtgaaaa ggtcttacat gggagaaact
     1141 taaatctact gaacgttttt ttattggaag gctttctagc ataaatgtgt agccataaac
     1201 ggcatttaag aaagtaaaga gtgctccaat aaatatagga acagaattcc ccaactcagc
     1261 taatagaccc cctataatag gacccagcgt tgttcctata ccgactaaag cacccatcca
     1321 tccaaaaact ttggttcttt cattaggttc cgtgatgtcg gcaaagtaag catataaggt
     1381 ggagatttct cctgccgtca atccttcaat gatacgacca aggaacaaca tccatatcga
     1441 atttcctaaa ccaaaaagca aatatccgat acttgatcct aataaactga taataaggat
     1501 cggatgacgc ccaaaatgat cacttaagct accgagaatg ggggcagata agaaggcagc
     1561 tagagcataa acagacatga gcaaagtgat atagaaagct tgctgatgta tggagtgaga
     1621 ataaggcagc gttaaaaacg gaagtacagg actgacgata gtaaatccta atccagtgaa
     1681 taagacagag acgtatccaa aaactttaga atgtttattt gtgaaactgt acatgtaaaa
     1741 actcctttat gcaataatgt taacttggaa acaaaaatag tgtatagtaa ttttgtttcc
     1801 ttgtcaacat tctaaggaac agcgaaatct acttaattta tttattccga aaagtgaaga
     1861 tacgcatcaa aaacatgaac aaacaaaaat caagtagacg acattgtaaa tctgaccgat
     1921 cagtgaagta aatagaaaag aaacctgttc ttgcttccca tgtttttttt acgtatactg
     1981 tgattgagcg gaggagaaat catgttgaat ttagaaaatt acttaagcgc tggacaaaca
     2041 gtcatatcta atcttttact taaaaattat cataggatcg gtctacaatc tgaagaattt
     2101 ttatttgttc tacagttgca tatggcacag cttgaaggag atactttccc tgatttgcag
     2161 atgattgcta tggatatggg aatcaaacaa gatcagattt ttcaaatact ggatcgtctc
     2221 gtaacaaatg gatttatcaa gatagaaacg acgattgtca acggtaaaaa agcagatcga
     2281 tataatcttt accccatcta tgatctatta ggtgagtatt taaaaacaca agaaaaaaaa
     2341 tacgaagaaa aaacacaaga aaaagaaatc cagtcagtgt atcaattatt tgaacaagaa
     2401 tttggacgcc cgctttcttc gatcgagttc cagcggatcg gacaatggct agaagaagac
     2461 cattatcagc cggaaatttt gaagctagca ctgcgggaag ccgtattaaa tcaggcttat
     2521 agttttaatt acgttgaccg cattttgcta tcgtgggaaa gaaaaaatct acgtacaaaa
     2581 caacaagtgg aagaagacca aaaacgaaga aaacagcagc ttcttcaaag agaagtagaa
     2641 cagtcagctc aacaagaaga attgccgaaa gtctcactga aaaattggtt ggaggagtag
     2701 cggaatatgt taagcaaaca aaaaacaatg gaagctctag aaacgatgta tggaatgttt
     2761 cccgaagcac atggagaact caagcacaat aatccatttg agttattgat tgcagtgatc
     2821 ttaagtgccc aggcgacaga tgtatcagtg aataaagcaa cgccagattt gtttgcctct
     2881 tttccgacac ccgatgcgtt agcagaagct tcaatcgatg aaatcattct taaaataaaa
     2941 acgattggtt tgtatcgaaa taaagcaaaa aacatcaaag cgtgtgcaca gcagctgatt
     3001 gaacgtttcg atgggcaagt gccaacgtca agagaagaat tgatgtctct tcctggtgta
     3061 ggcagaaaga cagcaaatgt cgtattgggg gatgcattcg ggataccagc aatcgcagta
     3121 gatacccacg tggaacgtgt ctcaaaacgc ctgaggatct gcaaactaga tgctacggtc
     3181 atggaagtag aagaaacact gatgcggaaa gtaccacaag aattatgggt aaagacacat
     3241 catacactga ttttttttgg tagatatcat tgcacggcaa gaaatcccaa gtgcgaggta
     3301 tgtcctttgc tatcaatatg tcaagatggg aaaaatcgga tgcgtctaaa agaaaaggcg
     3361 ctaaaaaaga aaccacgcct gtagacgtgg tttctttaat tgcaaataaa tgtatgaatt
     3421 atcttgttat tatggagaaa agctgtttcg tttatctttt ttgcatgaaa aagtaaatgt
     3481 acatactcag tagctaggat ggtaacttat tacaatttat tgcgataaaa ataaaagttg
     3541 ttatttttat tgggaaaata cccgatgtag aaaataatgc cttccaatat acctataaaa
     3601 taagtccagt attccaaagg attatataaa taagtgacgt atctatctaa aggtgtaata
     3661 aaaatgataa gtgataataa aaaaatacca tttataacta aacataaact aatggatttg
     3721 ggcggtttca ggctcatcag aaaaatatag attctggtga acaacaacca taaaaaagtg
     3781 agaatcattg catgagagct tctttccata aattttcctt taagagtagt gcttaaaagt
     3841 acagaaaaaa taataaatgc aaggatagga gcaattaaag taaatttatt taataataaa
     3901 caaaattttt cagctttttt attcttggaa taacgaggta ttgttttcga taaaaaaaga
     3961 tggtgcagac aaattatgat aattaagcat actatcagat aactcaaaat gaagccttct
     4021 ttcaaaaaat tattcaaaac catatattgt attttttatt tgataataac aatgggatta
     4081 ttaagaagtg gcactaaata aaagactagt gtgtatttag ccgatttaat tataaacaag
     4141 taaatggata aagtatatgt aatgataaaa gaaaaatgga ttttctaatt tttaagctaa
     4201 atgttagatc acacatgtat atgctggata gtcttataaa aaatcatgta atacagcttg
     4261 gtacatgatt tttatttgta gctgtttttt acatagacac aaaaaagaaa ccacgcttgt
     4321 agacgtggtt tcttttttac tatctgtttc cagaagctga ggtgctgctg gatggtgtat
     4381 ggtcatttgc agcatccgct ccgctagttg ctggctcact ggaagaagca ggaggagcag
     4441 atgaagacgg tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt gaagacgctg
     4501 gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc agcagttgat tcgctttgac
     4561 tagtagagct agagctttga gtcgttgtct ctgttgttga gcttccagga gtctggacat
     4621 agctggaaga tggaatagtt gttgatggtg taattgcatt cgatctagct gtatattgat
     4681 ctttgtagta cagttcaccg cccacacgaa ttaatccgct tggcatctcc cagtctgtat
     4741 ttgtcacgtt agctgaaaca tactgcataa gctcgcgata aacatcagag gctacgtgtg
     4801 acgattctga agtgacaggt gtcatttttt tattgtaacc tgtccacacg gaaatcgaat
     4861 aatttggcgt atagcccgca aataggatat ctggatacac cccagaagaa ataccaagtt
     4921 ttgcatactc gtcatctgtg taattggatg ttccggtctt accggcttga tataacccag
     4981 cgatttgcgc attcgtacca gttccttcag tgatcgtatc ttttaaaatg tctgtgatca
     5041 tataagctgt ttctggagac atggctgttt ttccatctgg ctcataagtt tcttctgttc
     5101 cgtcttggaa gacgatttta ttaacatatt gaggtttgta ataagtccct ccattggcga
     5161 aagccgcata ggctgctgcc atttttaagg aagaagctcc gtattttgtc ccatcttgtt
     5221 cttccgtatt actagagatc gcattcgatt gatggatcgt actgtattcg atcccaagat
     5281 tttttaagaa agaagccact ttatctgaac cgacttcatt gaataatttg actgctggaa
     5341 cattacggga taagtaaagt gcttggcgta aagtaatggt gcccatatat tgattatccc
     5401 agtttcctac aggagtcgaa gtcccttcat agttatacgg tgcgtcagta atagtttttc
     5461 cagtagaata tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt ttcatcgtag
     5521 aaccaaaatc acgggaagta ttcactgcta agttgttgcc taaagttacg tcttctgcga
     5581 tgtgacgtcc gccaatctgg gcttttactt tcccggtatt ggtgtcgata agggtggagg
     5641 caacttgcat ctcgtcgtct gggtaagaga catactgatc cgtgttgacg atatcatata
     5701 gtttcttttg ggcgtccaga tccaaattcg tataaatctc taaaccgtct gtgtagacat
     5761 tcttatctgt tttttcttgt acttcattga tgacttcttt gacataatta tcgacgatct
     5821 tagtattgtc atcggattga gttagttctt gcagaccatc cgttaccggt acattaacag
     5881 cttgatcata ttctgtttga gagatttttt cgttttgaag catcgtatac aaaacagtat
     5941 ctcgtctttt cttcgcctga tctggataaa cataaggatc ataggcggaa ggggcttgag
     6001 gcatcccagc aagcaatgct gtttgcggta aagaaagttc tgataatttc ttaccaaagt
     6061 acatctcaga agctgtctcc atcccgtaca agccgtttga catatacact ttatttacgt
     6121 agtaggtcaa gatctcttgt ttcgattttt tttgttccaa tcttacagcc atccacgctt
     6181 cttgggcctt gcgtttcaga gtctgatcct cggcactggt ggagaagaag gaaagtttga
     6241 tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag ttagagaggg
     6301 cagaaccgat gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg cgatcttcta
     6361 ccgacacgat cgcatcttct aatgttttcg gtaattcatt cgcagaaatt ttttcacgct
     6421 tttctgcgcc taaatcttcg aatagttcgc catcttgtgt atatagtttg gaagatacgg
     6481 tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac cagaaaagtc
     6541 ctacacctgc taaaaagagg atacagaaaa atgagagtat tcctaaaagg atcttgataa
     6601 ataatccttt tttatgtgtt ccagaggact ttccagaacc tttgccagaa ttcttcttca
     6661 cactcttttt aggagtaggc tgtttctgtc gtctagagct tctagtttgt tcatttgcca
     6721 ttcttgcttt ctccttcaat agataatgtt gataaataga agtcaaccgc gtccaaataa
     6781 ggaatccgcg gagaaatacc agttttgatc tctattccta gttcttcgat caaagataaa
     6841 ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa atgaacacta
     6901 ttcaaataaa aacagcgctt caaagacgaa aaccataaca agacaaagca gaccccattt
     6961 tgttcgaggc atgctttcat atgttcgatt tgatgggcat ggaagttttt aaagggaaaa
     7021 gatgttttgt tcttggtttc ttttgcttca aagtccaagt aaaagcctcg atagacgcca
     7081 ttatagtctg tagttgaggc ttgggtaaag taggcttcct tgatcacagc agcacttctc
     7141 ttaggatagt ctactttcac gatttggata ggggtaggct ttttatggat aaccgccaac
     7201 ccgcgggaaa gataatattt attactttca ttgattgctt cttcaaagcg catcccccga
     7261 ttgccgaatt cagtgatctg atttcttttt tcttgaattt tttcgacggg agaggcattc
     7321 ttataataag gttggccatt tggatagcgc aataccatct ttaatcacac tcctaggcca
     7381 cattatagca aaaaagcgga tagaaagaaa aggaatctga gatggataag tcaaaagtaa
     7441 tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt taaagaaaat gatcctaaaa
     7501 tccaagttgt taaaaatgtt ttaaaaaaag aactccatca gttagcagaa gaagggctgg
     7561 aatgggtact tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa atagtagcgg
     7621 aattgaaaga agaatatccg gaactatctc ttggtatttt gtatcctttt tctgattttg
     7681 gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa ttagcagatt
     7741 atgtcgaagc tgttagccat caaccctacc aatcacctag tcagctaaag aatcacactc
     7801 gttttatact tgagcataca gtgggctgtc tgctgatcta tgatgaagaa ttccctggaa
     7861 aaacgaagta tttttttgaa gatgcacaga attaccaagc agatcatccc tacgaaattc
     7921 ggctgatcag tattgacgat cttcaaaatt ttagtgaata atcgatattg tgtttgattt
     7981 atcaactttt ttcctataca atgaaaaagg tagaaatgtt attacgtaaa tgaaaactag
     8041 aggtgtaaat atggcgaatt tggtttatag tcctaaagac attttacaac aagaattcaa
     8101 aacaaaaatg cgcggctatg atcccgttga agttgatgaa ttcttagaca atatcatcaa
     8161 agattatgaa acttacagca aagaactcct tgctttacaa gaagaaaacg atcgattgag
     8221 tgcaaaagta gctcaattat ctaaaactca aggagcagct caaacacgcg tgcaacaaac
     8281 agaggcacca aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct tgtcaaatct
     8341 tgaacgtgaa gtatttggta aaaagcttga tcaacaagca agtgcagtca aaccagcaca
     8401 acctaatcca aataactaca caaatgcaga cacaagctta gatgataatg aaaaaacacg
     8461 tcaattttaa tttttgatta atgaatttgc ggttttcggg taatcgcggt ctgacttgat
     8521 caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc gtgcttagcg
     8581 aaaccataag ctaaggtact ctttggagta acggcaggaa aacaagctaa ggcttcggct
     8641 atgcttaagt atccttgaaa gtgccacagt gacgaagcgg ctggggaaac ccatccggtg
     8701 gaacgcggta aacccctcga gcgagcaacc caaacaatgg taggggcgct ttttgtctgg
     8761 aagaagaaca ggacaaaaag gcagtataac tgcagataga tggttaccgc tttgtttttt
     8821 ctccctggaa aagcaaagta cagaacatgg cttatagaaa accgcaaaca tcagggaatt
     8881 aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa acatttgaaa gctatttttc
     8941 aatacttcag atgaatcgtt cttattacaa aacaaacttt tgtatcaccg gttattcgga
     9001 atttgagtaa aagtgtgggg taggactgat gtcccacctc ctttttttat aaataaggag
     9061 attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa gcgggctaga
     9121 agcactggtt ggtaaagaat taagagatct tggaatcgaa tgccaagttg agaacggacg
     9181 tgcacgtttt gaaggaacaa tggaaacgat cgctacagct aatttatggt tacgtacagc
     9241 agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg aattgtttga
     9301 aaacgtaaaa gcattgcctt gggaagattt tcttccacta gatgcagctt ttcctgtagc
     9361 tggaaaatcg atcaagtcga aactatatag cacacctgat tgccaagcga tcacaaaaaa
     9421 agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca gcttctgtac cactgacgga
     9481 aactggcgca cattttcagt tggaagtagc tttgctgaaa gaccatgtga tggtaactct
     9541 ggatacgaca ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag gtggagcacc
     9601 attgaaagaa aacatggcag cagcacttat tatgttgaca aattggcgaa aagaccgtcc
     9661 attttatgat cctgtatgtg gttcaggtac gctttgtatt gaagcagcct taataggcca
     9721 taatatcgca ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt ggttcagtca
     9781 agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg atattgaatt
     9841 agatattact ggttcagata tcaatggacg gatgattgaa attgccaaag ccaatgcaga
     9901 agaaatcgga ttaggctctt cgattacctt taagcaacaa gcagtcaaag acttcaaaac
     9961 ggataaagaa tacggggtca tcgtggctaa tccgccttac ggagaacgtc taggagaaga
    10021 agaaactgtt cggaaattat ataaagaaat gggcgatgta ttccgtccat taaaaacatg
    10081 gagtaaatat atcttgacta gcgacttagc gtttgaggaa ttttacggac aaaaagcaac
    10141 caaaaaaaga aaattgtaca atggtgcatt gcgtacggat ctattccagt attggggaac
    10201 tcgtccgcct agaaaaaaag agaaagacga ataatcttac taattgattc attcaattgg
    10261 atgtttggag gaatagggaa tgaaagaaaa agagttttta caagaagtaa aagaaatcaa
    10321 tcttctacaa caagctttag gaatcctcga ttgggataca caaacaggta tgcctgaaaa
    10381 agctagtgca taccgaagtg aagtagacag ctatctttac agcatttatt tttcaaaaaa
    10441 aatcggtcca aaaatcaaag aagccattcg ctatttttct gaacatccgg aagaattgtc
    10501 ggaagttgga actgccgttt ttgccaaagt aaaagaagag tatgatttgg aacatcgcgt
    10561 accggaagat cttatgagaa aatatacagc tgccgtttct tcagcacacg gacaatggca
    10621 aaaagctaga gaaacacaac aattttctga ttttcaacac gcattgaatc aaaatattga
    10681 attaacaaaa cagttgatac cttactggag aaaagatgaa aagacgaatt atgatgtttt
    10741 gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt gataaggtat ttgatcagct
    10801 taaagaagga attctttcga tccgccgtac gctccaagaa aaagggactg aacctgaaac
    10861 agacttttta aatcggagaa tgacgaaaaa acaacagaag agatttgtag tgaaagtaat
    10921 cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc acccatttat
    10981 gactggaatt aatccgaatg atgcacgaat cacgacacga tggaacgaaa cagatttcaa
    11041 aatggctgtt tttggagtga tccacgaggc tggtcatggt atgtatgaac aagacatcga
    11101 tgataaatat gcgtatacac cggtttatga aggtacatca atgggtatcc atgaatcaca
    11161 atcgttgttt aacgagatca ttattggaag tagtcgcagc ttctggaaaa agcaatatcc
    11221 tttctttcaa gaatgtgcag aaggaacatt cgatgatatt ccttttgaca aattttatcg
    11281 ttcattgaac catacaaaat caagtttggt tcgtatcgag gcagacagtt tgacttaccc
    11341 tctccatatc atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag gggtagatgt
    11401 tgctgaacta cctgaaatat ggaatcaaaa atatgaagag tatctgggaa tacgaccaac
    11461 aaatgatgtg gaaggcatcc tacaagacgt tcattggtca ggtgcaagct ttggttattt
    11521 tccctcttat gcattagggt acatgtatgc ggcacaatta cttcattctt tgcagcaaga
    11581 aatagatgtg gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa actggatgtc
    11641 agagcatatc catcaatatg gtgcatctag aaaaccaaat caattaatca tggatgcaac
    11701 acatgaaccg cttaatccgc aatacctgat cgactatatg aaagacctgt attttaatgt
    11761 atatcaggta aaataaaata caatttacaa agatagctga aattcatcta atctatagat
    11821 gttttcagct atttacttaa gaaagaagtg gaacgaatgg ataaattaca agtgatcgct
    11881 gattatagta tggaccaatt agcgcatgat caaacaggac atgggagcga tcataccaaa
    11941 agagttgtca agttagctga aaaaattttg gagacggaac cgcaggcaga ccgctttgtg
    12001 acgcttgcag cagcttacct acatgatacg attgatgata aagtcgttaa aaatgagaac
    12061 gaggcaaagc aacagcttcg tgtattcctt cgctctttgc caatagtaga ggaacagatc
    12121 tcgatgattt tttccattat cgaaaatatg tccttttcta aaaatctctc tgaagcggcc
    12181 gaactatctc tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc attaggagca
    12241 atcggcattt tgcgtactgc ttatttcggc ggtggacatg ggcacccgat ttttgatcct
    12301 gaactgtatc cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac gacagtgatc
    12361 aatcattttt atgaaaaact gttcttgctg tctgataaaa tgaatactga ttacggatat
    12421 gaagaagcca aacgaagaga aaccttcatg cgtgaattct tagaagaatt ttttctggaa
    12481 tggtctattg acgatgaaaa ttttactcca gacaattgga tcaaaaataa caaaaaatga
    12541 tggaggttgt gacagaagtg gtcagctcca agatatttcg gatcattttc cgtaaaagtt
    12601 acttctggaa cactgtttac caagaggtcg tgagcctgcc gttcagctcc gagtcacaag
    12661 gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt gattcggaat
    12721 tggcaggaga acaccgttta ttcggaagaa taggactgtg acaagacttt tgtcacagtc
    12781 cttttttcga attcaacaag taaaactttt aagaaatatt cagagagtaa aattaaaaat
    12841 tgctgataaa cgaagatgga aattttatgt atttttattg gtctatgcaa taaataaatt
    12901 aaatattcag aatacaattg attttaacga aagggcttct atcaatggtt gtgttgtatt
    12961 tatctgccca ggtagagtaa aatagatcat tatctaaaag gtaattgttc atactatata
    13021 agtcatttga aagtaaaaag atactgtaaa taaaaaatgt ggttaaataa ttctcataat
    13081 cagtgtaagc gttatcaaaa atatagcata cttgctttgt agatgaatga cacgatatat
    13141 gtttggtatg ttctgcaaga cagatgaata tggaggtgga gacaatgtct tccaaagaac
    13201 taacaatgta tttagagaaa gaattggatc aactgaaaga aaaggggtta tacaatacga
    13261 tcgacatact agaaagtgaa aatggtgctt gtatcatcgt agacggaaaa aagatgatca
    13321 atctagcatc caacaattat cttggatttg ccaatcgcga agaattgaaa aaagcatgta
    13381 ttgaagcaac cgaaacttat ggtgtaggag caggagcagt ccgaacgatc aatggttcgc
    13441 tgaaaatcca tcagcaatta gaagaaaaaa tcgcagaatt caaagggaca gaagcagcaa
    13501 tcgcctttca atctggattc aactgcaata tgggcgctat ctctgcagta atgacaaaag
    13561 aagatgctat cttatcggat gaattaaacc atgcatctat tattgacggt tgccgcttat
    13621 ccggagcaaa aatcatccga attaaacatc aagatatgaa agaccttgag aaaaaagcaa
    13681 aggaagcgat agaaagtaag aaatataaaa aaatcatgta tatcacagat ggtgttttct
    13741 ccatggatgg agacattgca cgattgccgg aaattattcc gattgttgaa aaatatggac
    13801 tgatcaccta cgtcgatgat gcgcacggct caggcgtaac tggaaaaggt gcaggaaccg
    13861 tcaagcattt tggattaagc gacaaaatcg atatgcaaat gggcacgtta tcgaaagcta
    13921 taggtgttgt tgggggttat gtagctggat cgaaaacact aatcgactgg ttgaaagcta
    13981 gaagtcggcc attcctattt tctacttcgc taactccagg tgcagctgca gctgcattgg
    14041 cttcgattac tttgatgcaa gaacatccgg aacttgtcga aaaagtttgg gaaaatgcta
    14101 attattttaa agaagaattg aaaaaagtag gctataacat cggtatgtct gaaacaccta
    14161 ttactccagt cattttggga gacgaaaaag tgactcagac tttctctaag aagctgatcg
    14221 aacatgggat ttatgctaaa ccaatcgttt atccaactgt gcctcttggg acaggcagga
    14281 tcagaaatat gccgactgcg gaacatacga gggaaatgct tgatgaagca gtagcagttt
    14341 atcaaaaaat aggaaaagaa atggaaatta tttaactaaa tttagttttt tcgtctattt
    14401 aaaattagag aacatgttca cgcatatatt gttaatcatc tgaagatagt agtaaaaaag
    14461 atcagggagg atcatcatga aaaaagtatt ggtgacagga tgtttaggac aaattggttc
    14521 agagttgatc acacggctac gtcaagaact aggcagtgaa aatgtcattg cgacagatat
    14581 acgcatgcct gaagtcattg agaaaaacga tatctttgag atcctagatg taatggatta
    14641 cgaaaaaatg cgcagcatag cagaacaata tcaagtggac acattgattc atttggctgc
    14701 tcttttgtca gctgttgcag aagcaaaacc acaattagcc tggaatttga acgtgactgg
    14761 gctggtcaat gcattggaag tcgctagaga attccagctg aaatttttta cgccaagttc
    14821 aatcggcgcg tttggtccaa atactccaaa agatcatacc ccacaagata ctcttcagcg
    14881 tcctacaacc atgtatgggg taacgaaggt agctggagaa ctactatgtg attattatta
    14941 tgtgaaatat ggcgtagata ctcgtggtgt ccgtttccct ggattgattt cttatgcaac
    15001 cttgccagga ggcggtacga cagactacgc agtcgatatt tattattcag caatcaaaga
    15061 aggaaagtac actagtttca ttgatcaagg tacatcaatg gatatgatgt atatgccaga
    15121 tgcgatcgat gccattataa aactcatgaa tgcagatcct gatcgattgg ttcatcgaaa
    15181 tgccttcaat atatcggcga tgtcttttga cccggaagag atcaaagcat ctattcaaaa
    15241 aatcatacca gattttgaaa tggattatga tgtggatcct gtacgtcagt caatagcaga
    15301 atcttggcca aattcactag atgtttcttg tgcgcaagag gaatggggct tttcgccgaa
    15361 atacgactta gatgtgatga caaaggatat gttggaaaaa ctgaaagaaa aattggtaga
    15421 gacggtataa aacaagaacc acatccaaaa agttttttat acttttggga ttggctatgg
    15481 tatgaaaggg gacaagggat ggaagaatat caagccacgc cagtaaaaga agtaaaaata
    15541 gaaaatgaat taaaacgtac gatgggattt tttactgctt tatcgactgt tatgggcaca
    15601 gtgatcggtg caggggtatt ttttaaagcc gcaagcgtag cagaagttac aggatccgcc
    15661 agtttgcata tgttttcttg gtttctcggt ggtatgattt ctgtctgtgc aggactaaca
    15721 ggagctgaac tggcagctgc tattccagaa acaggaggga tgattaaata tatcgaacgg
    15781 atttacggaa atacagcagc ttttttattg ggctgggcgc aagtcgtgat ttattttcca
    15841 gcaaatgtag cagccttgtc gatcattttc ggtacacagt tcgtcaatct atttggtctt
    15901 tcccagtcaa tgattgttcc cgtagctgtt acagctgctg tatcgatcct gttgatcaat
    15961 tttttaggtt ctaaagctgg aggggctttt cagtcgatta cgcttgtctg caagcttatt
    16021 cctttgtttg tgatcgtcat tttcggtttg ttccgacaag aaggagtaga cttccaactg
    16081 tttccgatcc aagcaggaga aaatctttca tttttctcag ccttgggagc gggcttgcta
    16141 gcaacgatgt tcgcatacga cggttggata cacgtaggaa atatttcggg agaattgaaa
    16201 aagccggcga aagatttgcc aaaagcaatt tctttaggaa tcatcggaat catgatcgtt
    16261 tatttattag taaatgcggt gttcttaaga accgcatcta tcgacggagt cgcaggaaac
    16321 agcaatgcgg caagtgacgt tgcaaaaatg atttttggcg gcttcggtgg aagactggtc
    16381 acagttggta tcttgatttc tgtttatgga accatcaatg gctataccct tactggaatg
    16441 cgtctccctt atgttattgc aaaagaaaac aacctgcctt ttagcaagct ctttgctaaa
    16501 cttcatgata aaactaaagt tcctgtagcc gcaggtatct tagagctagt catcgctatt
    16561 ggcatgatga tgatcggtgg ttttgataca ctaacggata tgctgatctt tgtcatttgg
    16621 attttttata cgatggtctt tgttggtgtc attcttcttc gtaaaaaaga acctgatttg
    16681 tttcgaccat acaaagttcc aatgtatccg tttattccac tagtagcaat cattggaggg
    16741 acttttatcg tatcaagcac gcttatcact cagacctttt tagcttcgat ggggatagta
    16801 ttgacacttg ctggtatccc gatttatctc tatttaaaac gtagatagct aaaacattcg
    16861 aattttatta gaactgcact tctctgccaa acaaaatatt ttatttaaat gaggagtacg
    16921 tggaagaaat gtcaaaagta ctcgttaatt ttgatcacgt aaatgtcgtt ttgtctacga
    16981 tgaaatagca aagtaaaaga cagaaatcgg gctcaaccga tttctgtctt ttactttgat
    17041 ttgcgtaact gaactattcg gcgtacgccg cgataaatat ttaataaaac caagcaacca
    17101 gaaatgaaaa tgggaagcca aatcagatag acagggtagc caagcacttt agacaaagca
    17161 tgtggagtca ttttatccat ttcaagaagt gcttgctgcc aagtctgaag attgaagact
    17221 agcatgaaac ccgaaataat caggagaata aatcctaaat aatgaattgt agttcttttt
    17281 cttacataag cgatcgtagc ataaaggaac agtaaaaaaa taggtacgcc aaaaacaaag
    17341 tacatattca gcatctcgac accccctttg tgtttatctt actttacttt gaaagaaatg
    17401 agaaataata gcctcgttac ttgcttgaaa cagcaaagga ctgctaagat aagagagata
    17461 caaagaatca aggaggagtc atatggcaag ttatatcgaa ccgattcgtt ttgcgctgat
    17521 tgtttttcca tttttagcct tagcgatctc atctgttttt tttatttatg aatatcgaaa
    17581 gtatggaaca tttctagtaa caagggcagt tattttgtat tctttcgttt tttatttatt
    17641 gtgtgcgtat tttttggtga tcttaccact tccggcaaag gagacggttg cccaaatgac
    17701 agggccaaag atggagctgc atttatttgc ttcatggcaa catttcagag acaacacggt
    17761 attggtcttg tctgaaccta gtacctacct gccggcaatg aagcagagtg tatttttgga
    17821 accagctttt aatgttttat tactcatacc atttggcatt tatttaagat attatttccg
    17881 gctttctttt atgaaaacga tgattgctag tttctgcctt tctctatttt ttgaattgac
    17941 gcagctaagt ggactttatt ttatctaccc aagaccatac cgcttatttg atgtgaatga
    18001 tttagtgaca aatacattgg gtggaatcat tggttttgtc gttacgccgt tatttacatt
    18061 tatgcttcct acaagaagac gaatggatga actttcttat gaaaaaggaa agactgtctc
    18121 attgactcga cgcttggtcg cttatttgat cgactggaca tttttatctc ttattaattt
    18181 ggtagcggcg attttcttta aaattgcgtt cgattcaggg gcagttactt ctcatatctg
    18241 gtggctttta ttagaagtag gggtatactt tatgattgta ccgtttataa ccaatggatg
    18301 gacacttggc aaaaaaattg tgcgtatccg tttggtggaa gaagaccgcg aacgaatttc
    18361 tttaaaagca ttgatgatcc gctacgggta tctgtacttt cttttttatg gtttaagctg
    18421 gttggggcag ttgcttcagc aaatcatgaa ttcgacaacg aatacggtgt tgcagatgag
    18481 tgcagtattc atttctctcg gtgtcttgtt actacaaggt atttttattt taaatatctt
    18541 gctatcattt ttgagaaaaa aacggattct gttttatgaa aaagctagtc acacatatac
    18601 tgttagcacg attggccaaa caaaggatga tgattctcga aattagctga gcaaaaacgc
    18661 tttgtctgtg ttataatgcc aaagtcgtga taatttaaag agaagaggtg cacgaatgaa
    18721 taattttgat atattccgct tcaaaccgtt tatcaatgaa gcgttaaccg caaagggctt
    18781 caaacagccc acagaggtcc aggaacgttt gattccttta attgcaaaag gaaaaaatat
    18841 cattgggcaa tctcaaactg gtacaggaaa aacacatacg tttttgttgc ctttgtttga
    18901 aaaaatcaat gcagaaaaag aggaagtaca agttgttatt acagctccaa gtagggaact
    18961 agctgcacag atctatgaag cagctacaca aatcgctaaa ttttctacac cggagatccg
    19021 tgtctcgaat tttgtcggcg gtacagacaa acagcgtcaa ttaaatcgtc tgcatcacca
    19081 acagccacag gttgtagtag gcacgcctgg tagaatatta gacatgatga atgaacaagc
    19141 gctgaagatt cacaccgcat ttgcatttgt tgtcgatgaa gcggatatga cattggatat
    19201 gggtttttta aatgaagtag accaaattgc aagtcgatta ccagaaaagc tgcagttttt
    19261 agttttttct gccactattc cagaaaaact tcgtccattc ttacgaaaat acatggaaaa
    19321 tccaatcatt gaagaaatca aaccaaaagc agttatttct gaagacattg aaaattggct
    19381 gatttcaaca aaaggtaaag atgagaatca aatgatttat caactgctga caattggtca
    19441 tccttattta gcaatcgtat ttgccaatac aaaacagcat gtggatgaaa ttgccgatta
    19501 cttgaaaaac caaggcttga aagtagcaaa gattcatgga gacatcaccc caagagagag
    19561 aaaacgagtg atgcgacaag tacagaatct ggagtatcaa tatgttgtag cgactgattt
    19621 agctgctaga gggatcgata tcgaaggtgt ctctcatgtt attaatgcag aaattccaga
    19681 agatctagac ttctttatcc atcgtgtcgg acgtactgga cgtaatggct taaaaggaac
    19741 ggccattacg ttatattcgc caaatgatga gcaagccatt gatgcgatag aaaaattggg
    19801 cgtctcattt gaaccaaaag aaatcaaaaa cggggaaatc gtcaaaacat acgatcgtaa
    19861 tagacgtgcc aaacgagaaa aatcaaaaga tgtattagat cccacgctga ttggtcttgt
    19921 gaagaagaaa aagaaaaaga tcaagcctgg ttataagaaa aaaatcgatt gggcaattgc
    19981 agaaaaaaat aaaaaagaac gcaaaatcga acgcagacag caaagccgta ctgctcgtaa
    20041 aaataagaaa aattcacata aataaaaaac aagaggttgt gacaaaaggc gtgtcacaat
    20101 ctcttgtttt taagctagac tttttttcat tccgatacaa agtacttcat cttcaataaa
    20161 aggttctgtg caagtatgat acccttgttt ttcataaaaa tctacagccg tcatttcagc
    20221 tactacataa ctagtctgac aaccttctga aaccgcccgt ttttcagcag cttcaagcaa
    20281 ctgaccgcct aacccttttc ctcggtattc ttttgctacg caaaatcgtt ccggcttgaa
    20341 gcaagtaggt gttttctttt cataacgcaa agtagctact ggtctttttt gatcaagaaa
    20401 tagataatag gtttgttgcg ggatatcttt tccgtcgatt tctagttctg caggaatccc
    20461 ttgttctaat acaaaaactt gatatctcaa aaagcagcta gctgcgcgta tccattcatc
    20521 cgctccaact atttttttca tcatattcct ccttgtcatg aatagcagag atgtattgat
    20581 tcctttattc ttatcgtacc aaaaatcgtg gagtatgacc aaaaggaaat gaaaataaaa
    20641 attgagatat tgacagtgat caatagaatg cgtatactaa ttaagtacga aaaggatatg
    20701 atttgttttt taagactcac agaaaattct ttcttggctg aaaaaagaat agtcactaat
    20761 caaattgctc cctttttcta ttttggtaga aataccgacg cagctcagcg ttatcgagtt
    20821 taagaggtaa tgattacaca tcattgcaat caaggtggta ccgcgagttt tcgtccttgg
    20881 ctgtgatggt gtttttttgt atttaattta tagaaagcag gtaatgacat gaaacaatta
    20941 agtagtgcac aagttcgaca aatgtttctt gatttcttcc aatctaaagg acatacgatt
    21001 gaacctagtg cgtctttgat ccccgtgaac gatccaacct tattgtggat caactctggt
    21061 gttgcaacat tgaaaaaata ttttgatggt tcagttgtgc cagaaaatcc aagaatcaca
    21121 aatgcccaaa aatctattcg aacaaatgat atcgaaaatg tcggtaaaac agcacggcac
    21181 catacgatgt ttgagatgct gggtaatttt tcgattggtg actatttcaa aaatgaagcc
    21241 atccactggg catgggaatt tttgactgat ccaaaatgga tggcattcga tccagaaaaa
    21301 ctttatgtaa cagtttatcc aaaagataca gaagcaaaac gcatctggca tgaagaagtt
    21361 ggcctgccac tagatcacat tattgatgta gaagacaact tctgggatat cggtgcaggc
    21421 ccatgtggtc cggatacgga aattttctat gaccgtggag aagcttataa cgatttagca
    21481 gaagacgatc ctgaaaacta tccaggagga gaaaatgaac gttatttaga aatctggaac
    21541 ttagtatttt cagaattcaa ccataagcct gataacacat ttgaaccact tcctcataaa
    21601 aacattgata cggggatggg attggaacgc atggtctcaa tcgttcaaga tgcgtcaacg
    21661 aactttgaga cagacttgtt tatgcctatt atccacgccg tagaaaagct aagcaaacaa
    21721 gtaaaatatg gtgaaaaccc agtaacagat atttcttaca aagtaatcgc agatcatatt
    21781 cgcgcccttt catttgctat tggcgacggt gcattgccat cgaatgaagg gagagggtat
    21841 gtcttacgcc gtttgcttcg tcgtgctgtc atgcatggga aaaaattagg tattgaacaa
    21901 gcattcttat atcgtttagt ccctgttgtt ggggaaatca tggtaagtta ttatcctgaa
    21961 gtacttcaac aaaaagaatt tattgaaaaa gttatccgta cagaagaaga acgtttcaat
    22021 gaaacaatca atgaaggttt ggaaatctta aatcaagtga tccaagaagt aaaaaacgaa
    22081 aacgggacga ctctagcagg aaaagacatc ttcaaactat acgacacata tggcttcccg
    22141 gtagaattaa cggaagaggt ggcagaagat gaaggactga aagttgatca tgctggtttt
    22201 gaaaaagaaa tggatgcaca acgcgaacgt gctcgctctg cccgcagtac tgaaaaatca
    22261 atgggggtac aatctgcttt attgacagat atcaaagtag aaagtaaatt cattggatac
    22321 acagaacaag aatcagatag tcaattattg gtcatcgtta aaggtgagtc tcttttgaat
    22381 gaagtcgcag aaggtgaagc tcaaatgatc tttgatcaaa cacctttcta tgcagaaatg
    22441 ggtgggcaag tagcggatca aggatggatc atgaatgaag taggcgaaat cgtagcagtt
    22501 gttacagatg ttcaaaaagc gcctaatggt caatttatgc acaccgtaga agtaaaagca
    22561 ccaatgagtg aaggacaaac ctatcatcta ttgatcgatc aatcgatgcg caatcggatc
    22621 atcaaaaacc atacagcaac ccatctactg cacaaggcat tgaaagaagt acttggcgag
    22681 catgcaaatc aagctggttc tttagtcgct cctggacatt tacgattcga ctttacccac
    22741 tttggtcaaa tcacttcaga tgaattagct gaaatggaaa gaatcgtcaa cgaaaaaatc
    22801 tgggaagcta tcccagttga aacaattgaa acagatatcg atacagccaa aaacatgggt
    22861 gcaatggctt tgtttggtga aaaatacgga catgacgttc gagtagtaaa tattggcggc
    22921 tggtcaatcg aactgtgcgg aggcacacat gtagcaaata cagaaaacat cggaatcttc
    22981 aaaatcgttt ctgaatcagg aattggtgca ggcgtgcgac gaatcgaggc tgtaacaagt
    23041 aaagaagcgt acgaactact tgaagaagaa gaaaaacaac taaaacaagt agcaacaatt
    23101 gttaaatcac ctcaattgaa agaagttgtt tcaaaagcgg aacaattgca acagcaagta
    23161 agagagctac aaaaggaaaa tgaacaatta gctagcaagt tagcgaatca acaagctggt
    23221 gatgtattca aagatccaga aacgatcaat ggaaaaacaa tcattgcagc acaagtaaaa
    23281 gtgaaagata tgaatcagct gcgtcaatta gctgaccaat ggaaacaaaa aaatatttca
    23341 gatattcttg ttttagcaaa cgaacaagaa ggaaaagtca gtctgctagc ggcaatgacg
    23401 aaagaatcaa atgaagctgg actaaaagct ggcgacttga ttaaagcaat cgcaccgaaa
    23461 gtaggcggtg gcggtggtgg acgtccagac atggcacaag ctggcgggaa aaatcctgct
    23521 ggtattccag atgcacttaa agctgcgaaa gaatgggttg cgcaacaata gggcgtttac
    23581 gtttgacaga aaaacaaaag ttcaaacatt gaacgaaaca tgattatggg ggcgaagaca
    23641 aacaagtctt gccctcatac ttttttcaat tggatggcgc atctaatcaa atggtcggat
    23701 tcggatggtc tttagttgac gaaagtgaca gaaattttta cgattagtat aaataaaaag
    23761 gggtgaaaaa atgaatagta gaaataagac ctttcgacta gtcttacgtg cgatcttgtt
    23821 agcaatcatc atcgtacaag cgatggttcc ttggctgggg tttattccat taggattcat
    23881 tagtttaaca attatccata tcacagtaat tattgctgca gtagtattag gcccaaaaga
    23941 tggcatggtg attggcttgt tttggggaat cgctacaatt gttcgtgcct atgccatgcc
    24001 aacaacgcca tttgatacat tagtgttcac gaatccgatt atctcggtag ttcccagagt
    24061 acttgttggc ttagtagcag gcttggtttt ccactggatt tatcaaaaaa ataaatcaat
    24121 aacgatcagc tcagcctttg caggggtact gggttcattg gtcaatacag tcttagtgtt
    24181 aggattcatg ggattatttt atacaggtgc aacggcagat gcctatggtg tagactctgc
    24241 tcttttattt aaaaccttag caggggttgc agcaatcaat gggataccag aagcaatagg
    24301 tgcgggaatc ataacaccat tactagcaaa agcgttattt gcagcaacac cgttgaaacc
    24361 agaataatga ccatctttta atagtaatag gacgcacgat agatagaaaa aggaagagag
    24421 gagaggaaaa caatcgtcct ctccatcttt ttacctgagc aacaagcaaa ctagaagggg
    24481 taaaaaactt ggctaagaaa caagaagaaa aaaagaaaca aataccgcaa cagcggacaa
    24541 aaaaaggagt actagcgggt atcttaggat tggttacgaa tctgatcttg tttgtctcta
    24601 agctaatgat cgggcttttt tcaggaagtg tctccattat ggctgatgca gtgaacagtc
    24661 tatcagatac tgcttcatcc atattgactt tgatcggatt taaaattgct gcaaagccag
    24721 cagatcagga acatccttat ggacacgaac gcttcgaata tatcagtggg ttatttgttt
    24781 cgatcatcat tacttatgta ggatttcaat ttttagagtc ttctgctgaa aaaatcatcc
    24841 atccagaaaa tgtctcttta acacccatcg tcttttttgt cctaatcatt tccatctttt
    24901 tgaaatttgc acaaggcaaa atgtatcagg ttattgcgca atccattgat tctgaaacat
    24961 tgagagctac agcaacagat agttataatg atgtctttac tacactggct gtattgcttt
    25021 cagcagcaat cgaatgggta actggatggc gtattgatgg atatataggt tttgctttag
    25081 cattatatat cctttatagt ggattgacga tgcttcgaaa ttttgtatac gaactattag
    25141 gaagtcgccc aacgccagca gagattgaag aaatggaaga gcatttaaat gcttgcgatg
    25201 atatacttgg ctatcatgat ttattggtgc ataactatgg acccaataaa cgctttgcat
    25261 cagtccacat cgaagtagat gaatcaatga atttgaatgt ggctcataag attattgatc
    25321 tgatagaaaa agactttaaa aagtctttgg atgtggaact tgtctgtcac ttggatccaa
    25381 tgcctgttga taacgaaaaa tactataata tcttgcggca gttaaagcaa attcttcttg
    25441 aaacaggtac agggcttaat attcatgatt ttagagtaat tcatcaagga aaaacgctgc
    25501 aatttgatgt agtggtccct gagaaattcc agtttgttga tacagaacta gaaagaatct
    25561 tacgtagtca aatagaagaa aaaatcggag attaccaatt ggaaattacg tttgatcaca
    25621 attatctttt atattaaatg agaaactata aaaagactcc ttaaagtttt taatttaaag
    25681 agtctttttt tcttttaatt gtttatggag agcctgacgt gcaagattat ctgctccttt
    25741 atttttgttt tcgggcaacc attgcaggat caataaagaa aattcggata gaagttcttg
    25801 gattctttga aggtagggaa ggaagagctc gttcttcgtt gcgtttttgt cgaccgtaga
    25861 aactagtgtt ttgctgtctg aaaaacagaa aatcgtttca ttctgtaagt cgtttttctt
    25921 tagaaattct aaagttttta aaaagacttc gaactctgct tgatggtttg tccctataga
    25981 aagcgggata gatagctgtt cttgtttacc ttgatagaca atcaagattc cacctccact
    26041 aggaccaggg ttgccttttg tagaggcatc aatgtatact tttaacataa aaagagattc
    26101 tcctttttgt tcaaactaat gttatggtaa gattatatca ttactgaatc ggtgagaggt
    26161 tagagaatga aaaaaaatat tcgatggcag cctgaactgg cacatacgat catctattgg
    26221 tcattgacct tgatcattct gttttttagt ctgattcttt ctttagaaaa cacaggtcct
    26281 tattggaaaa gcaattttgt tttaggtatt tttcttgttt ttctatttct aggtttgcgg
    26341 cgttctttta ttttaaaatc aaactctgtg attatacgtt atgctgcttt ttggaaagac
    26401 cgagaaatat ctgtcggaca gattcgatct ttgacgccaa ctaatactgg ggtgcaaatg
    26461 atgttgcaag atgagaaagc cccatctgtt ttcatgatgc gaaaaaaaga tcaagtagtc
    26521 ctttttgatc attttgaaaa aaacggaccc aaatcagaat caattcctta tccttatacg
    26581 aaaaaaagaa aaagctaatt ttaaaaatca gaacctaaaa aaacgaattt ttacaggaaa
    26641 agaaaagagt gagacgtacg gttcatcccg tttagcgtct cactctattt gtttatctat
    26701 tcatgcatac tagtatcggt agtctgaacg ttgcctttct tgacatgggc agcttgtgtt
    26761 cctcgattcc cttcgatgat ctcaaattcg accacttgat tttcttccaa agttttgaat
    26821 ccctcttctt caatcgcagt aaaatgtaca aaaatttctt ctgtgtcatc ataactgata
    26881 aagccgtatc cttttttgtt gtcaaaccat tttacaattc ctgtggtcaa agcactcacg
    26941 acctttctaa agtctgatca tagccttcat tataaagagt atttataagt acgtcaaata
    27001 aattaaaaac ctgtaatttt atgttttttt tctgaataaa aaaaggtata attggcatat
    27061 cttaacaaag aagatatttt gttgaataaa gttcgtgaaa aggggtaatc aaatgaagaa
    27121 tgatctgcaa atttcacaag aaacagagct agaaccgatt gttgaagtgg ctagtaaatt
    27181 agatcttaag gaagacgatt tggaactgta tgggaaatat aaggcgaaaa tcaatttttc
    27241 tggaatcaat cgatcaaagg ataacgctga ggggcatttg atcttagtta cttccatcaa
    27301 tccaacacca gctggcgaag gaaagtcaac gatcactgta gggttaggag acgcgctcaa
    27361 taaaatcaat aaaaaatctg ttattgcatt gcgagaaccc tcacttggtc ctgtgatggg
    27421 aatcaaaggt ggtgctgcag gcggaggata tgcacaggta ttgcctatgg aagatatcaa
    27481 cttgcatttt actggtgaca tgcatgctat cacaacagct aataatgcat tatctgctct
    27541 tttagataat catattcatc aaggaaatga attgaacatc gatgcccgcc gagtgatttg
    27601 gaaacgggta gtcgatctca atgatcgtga actgcgtaaa gtaattgttg gcttgggtgg
    27661 gccaatccaa ggtgttccaa gagaagatgg gtttgatatc acagttgcca gcgaaatcat
    27721 ggctatcttg tgtcttgcgg cagaccttga agatttaaag gctcgtcttg caaggatcgt
    27781 tgtcggctat acatttgacc gtcagcctgt gactgctgga gacttgaaag cagaaggcgc
    27841 tctggcatta ctgctgaaag atgcaatcaa gccgaatctt gtacaaacaa tttacggaac
    27901 accagctttt gtccacgggg gaccgtttgc caatatcgct catggctgca atagtatttt
    27961 agctaccaag acagcattga gattagcaga ttacgttgtc acagaggcag gatttggtgc
    28021 agatcttggc ggagagaaat ttttagatat caaagtacct aacttgaaaa aagcgccgga
    28081 tgctgtggtg attgtcgcca ctgtccgtgc attgaaaatg catggcggaa tgaaaaaaga
    28141 tgaacttaaa aatgagaatc ttgatgcttt gaaagtaggt tttgcaaatt taaagcgcca
    28201 tatacgtaat atggaacaat atcagctacc tgtgatcgtc gcaatcaatg aattcgtaac
    28261 agatacagac aatgaactca cactgttgga gcacttgtgt gaaaatcagg ggattcttgc
    28321 caagagagcg agtgtatggg cagatggagc agaaggcggc gttgatttag caaaagctgt
    28381 agtacgattg attgatagaa aagaagctaa ttacaagcca ttataccgcc tagaagaaac
    28441 gatacaagaa aagacagaaa caatcgtcaa gaaaatttac ggaggaaatg gtgttgtttt
    28501 cagcaaaaaa gcgcaaaaac aaatagaaga gtttacgaag aatggatggg acaaactgcc
    28561 gatttgtatg gcaaaaacac agtattcatt ttctgatgat ccttcactgc taggtgcacc
    28621 ggaagatttc accatcacga tccgcgaaat catcccaaaa ttaggggcag gatttttagt
    28681 agccttgact ggagatgtta tgacgatgcc tggtttgcct aaaaaacctg ctgcattgaa
    28741 tatggacgtg acaaatgacg gagaagtgtt gggattgttt taagcttgat tataatctac
    28801 catcgaagga gctgtatttt gactacagct tcttttttta gacgataggg aattaatttt
    28861 gtttacacta ggatcattct ttttctttat tgtattttga gctatactaa agcataatag
    28921 gagggtatga tgaaaacgac atttataaga aaaaccggtt tttacggtat gggtagctcg
    28981 attcagctaa gaatcaacaa tgaaaaacgc agtgttctca gtcatgaaca aaagataacg
    29041 cttgatctgg aagtgccctt tacgatgcag gtcacctttt attggctaaa aagccccgtt
    29101 tatacagtga aagaagcaaa gcctgcttat gtgatcacaa tgaattactt gattcttcaa
    29161 atctatccgt ttttatttct agcaactggg ttgggggccg ttttcctaca aaatttattt
    29221 ttcagttttt ttgcagttat tgtgttgatt gcgttctttt tctatataaa aaaccgggca
    29281 tatttgatca aggaggaaaa ttatggcaaa ctctgatctt tttttaatca gtttcaatcg
    29341 aatcgaaaaa tggatgagag acgagatggg aaatgcacga aatatgggat ttaccgaact
    29401 tgtcagacgc ctagctcaaa gaaaacaatt aatgatcaga aaatacgaag atgatctttt
    29461 acagcttgct cagttaagga atgcgatcgt ccatgatcgg atcgcagtcg attttgtcat
    29521 tgcagagcca aatgattgga cagtcaagcg aatccagctg atcgaacaag aactgattca
    29581 gcctgaaacc gtgctgcctc gatttgcaaa acgtgtaaca ggttttgaac aagatcttcc
    29641 gttacttgaa ctgttaaaga tagtagcaga aaaaaggtat tcacaatttc cgttatacaa
    29701 taaaggaaaa tttgtagcat tgattacttt acgtaatatc gggttttggc tagcaaaaga
    29761 aagccaaaaa ggtccggttg atttaacaaa taaacgagcg ttggatttga tcatccaaaa
    29821 cggaaagtac acaaattatc attttgttcc agctaaaaca catatttacg aggttgaagc
    29881 gatgtttcgt gagcaaggga ctttggaagc cgtacttatc actaaagatg ggaatcccga
    29941 tggtaatctg ctcgggatcg tacgacctcg tgatatttat aaacaaatag aaaaggattg
    30001 attttttgtt aattatttat tggatattca gcgctttgct agtaggagtg gatcagttgg
    30061 tcaaatggtg gatcactgat aatctagcgc ttggagagac gaaaaatctt ataccaggta
    30121 ttttgtcact gaatcatatt cgaaacactg gagccgcatg gagtatgcta gaagggaaga
    30181 tgtggttctt tactatagtg acactcattg cagtcgttgt gattctcaca ttgatgatta
    30241 aaaatcgtga aaaaggtaat cgctggttca tgattggatt gagtttgatt ctagctggtg
    30301 cgattggcaa ttttatcgac cgtattcgtc ttggctatgt agtagatatg ttccagacag
    30361 attttatgag ctttccgatt tttaacgtag cagatgtgac acttgtatgt ggagtgattt
    30421 gtatgttaat ttatattatt ttagatgaaa aggatcaaag aaaaaaatga cagaacttaa
    30481 aacgacgatt caccatgaat ctggtaggat cgataaagta ttgactaatt tattcaccga
    30541 ttacagtcgt tcgcaaattc agctgtggct gaaaaatgga gcggtttctg tcaatggaca
    30601 gccagtcaaa gcaaattaca aagtaaaaca aggagacgag attactgtct tgatccccga
    30661 accagaaaca ttagatgtgg tcccagaaaa tatcccgttg acgatcgttt acgaagatga
    30721 agatgtagcg gtgataaaca aacctcaagg aatggtcgtt catccttcag caggtcatac
    30781 gcatggcaca atggtcaatg cgctgatgta ccatatcaag gatctttcta cgatcaacgg
    30841 cgttattcgt ccaggtattg ttcaccgaat cgacaaagat acttccggac tgctaatggt
    30901 tgccaaaaat gaccaggcac acgaatcatt agctcagcaa ttaaaagata agacctcttt
    30961 gcgcaaatat gttgctttag tccatggaga gattcctcac gaaaaaggaa ccatcaatgc
    31021 gccaatcggc cgttcgaagg aaaaccggaa aatgcaggcg ataagagaag atgggaagtc
    31081 ggctgtcaca cattttactg tattggaacg tttcgaagga tttactctgg ttgaattaaa
    31141 actagaaacc ggaagaacac accagatccg tgtgcatatg aagtatatcg gttatccgtt
    31201 agctggagat ccagtatacg gaccgaagaa aactttgaaa ggcaatggtc agtttctcca
    31261 tgcgcaaatg ttaggtttta cgcacccgac tacaaacgaa aagatgatct ttgaagcacc
    31321 tcttccagaa gtttttgaaa aaactttaga aaaattaaga aaaaacattg cattttaatt
    31381 tagataacgg tatagtaatt aacaacgaaa gcataaaact taatagaatt cctttaagtt
    31441 tagtcccgtg aggctaagaa gggtgggagc aaggatacgt atggtgcgcc ataagtagat
    31501 tgctcattga catagtatag ctatcagtgt atcagtatac gctcagtcta aacattcctc
    31561 ctgtcctctt agggcaggag tttttttatg caaaaaagta gggggaatga aaatgcaagc
    31621 aaaagaagta gtagatcagg taaccatgaa gcgagcattg actcgaatta cttatgagat
    31681 tatcgagaga aaccatagta tccaagatat cgtattggta ggtatcaaaa cacgaggaat
    31741 ctacattgca tcaagaattg ccgaacgcct gaagcagcta gaagatatcg atattcctgt
    31801 aggagaactg gatatcactt tataccgaga tgacaaaaaa gaaaatccag aagaaccgga
    31861 acttcattct tctgacatcc cagtttcctt agaaggaaaa gaagttattt tgattgatga
    31921 tgttttgtat acaggacgaa cgattcgagc agcaatggac gcagtaatgg attttggcag
    31981 accaagaaaa atttcattag cagtcctggt cgatcgtggg catcgagaat tgccaatccg
    32041 tgcagattat gttgggaaga acattccaac atctcgggcg gaagagattt tagtagagat
    32101 gcaagagctt gatggacaag atcgcatcat gattttgaaa gaggaagatt agaggagaga
    32161 gaaacatgtc ggagaaaaaa ggatttcata atgatgacgt ggtgttagat atccacgatc
    32221 gcccaacccc aggacattgg gtaggattaa gtttacaaca cttgttcacg atgttcggag
    32281 caaccgtgtt agtccccatc ttagtaggta tcgatccagg tattgcgcta gtcagttcag
    32341 gactaggtac attggtgtac ttatttacta ctaaaggaaa gatacctgcg tacttaggaa
    32401 gcagcttcgc tttcattgca gcgatgcagc ttttgatgaa aacagatggt tatccagcga
    32461 ttgcacaagg agcagtcaca actggattag tctatttgat tgttgcgctg atcatcaaaa
    32521 agatcggttc agcatggctg gataaaatct tgcctccaat cgtcgtcggt ccagtcatca
    32581 tggttatcgg gttgggactt gcggcaaatg cagcaacaaa tgctatgtat aatcagggca
    32641 cgtatgattt caagtatatt gctgtagcgc tgatcacatt aggattaaca attttcttca
    32701 acatgtggct aagaggtttt ctcggattga ttccgatttt actaggtatt attagcggat
    32761 acatcgtagc cttgctgttt ggaatcgtgg atactaagcc aatcgtagac gctgcctggt
    32821 ttgctgttcc taactttgag attccctttg tacaatatga accaaagcta tatcttggag
    32881 caatcacaac aatggcaccc atcgcttttg tgacgatgac agagcatatc ggccatctta
    32941 tggtattgaa taaactaact aaacgtaatt tctttgaaga accgggtctt cataagacgt
    33001 tgacaggtga tggattagca caaatcgtgg cagcctttgt cggaggtcct cctgtaacaa
    33061 gttacggtga aaacattggt gttctggcta tcacacgtgt ccatagcgtg tttgtgatcg
    33121 gtggagctgc agtatttgca gtcattttag gattcgtagg aaaattaagc gctttgattt
    33181 taagtatccc tggaccagtc atctcaggaa tcagttttat cttatttgga gtaattgccg
    33241 caagtggaat gaagatttta atcgagaaca aaatcgattt tgataaaaag aaaaatctgt
    33301 tgattgcttc agttatctta gtagtaggaa ttggcggact cgtctttgaa gtaggcacat
    33361 ttacattgtc ggcaatggca ttagcaactg tacttggcat cattttaaat cttatcttac
    33421 cagaaacttc acgaagcgaa gaaaactaag gaggaaacat tatgatcatc aaatcagaac
    33481 gtatcagctt aaagcattta ttgaccgttg aggcacttac tgatcaagaa gttatgggct
    33541 tgatccgcag agggcaggaa ttcaagcaag gtgctaattg gactccgcaa aagcgccagt
    33601 actttgctac aaacctcttt ttcgaaaata gtactcgcac acataaaagc ttcgatgtag
    33661 cggagaaaaa actagggcta gaagtgattg aatttgaagc aagcacaagc tctgtccaaa
    33721 aaggggaaac actatacgat acagtactga cgatgtcagc attaggcgta gacgtagcgg
    33781 ttatccgtca tggtgatgag aactattatg acgaactgat acaaagtaaa acaatccagt
    33841 gttcgattat caatggagga gacggaagcg gacagcatcc aacccaatgc ttgcttgatt
    33901 tgatgaccat ttatgaagaa tttggtcatt ttgaaggtct gaaagttgcc atcgtaggtg
    33961 atatcactca ctctcgcgta gctaaatcca atatgcagat gttaaaacgt ctaggcgcac
    34021 aagtattctt ctcaggtcct agagaatggt acgacgatga attcgaagtg tacggacatt
    34081 atatgccatt ggatgaacta ctagatcaag ttgatgtcat gatgatgctt cgggtgcaac
    34141 acgaacgcca tgatggaaaa gaaagtttct caaaagaagg ctaccatcag gaacatgggt
    34201 taacagttga gcgtgcgaag aagttgcaaa aacatgcaat cattatgcac ccagcaccag
    34261 ttaaccgtga tgtagaatta gctgattcat tagtggaagg attacagtcg cggatcgttc
    34321 agcaaatgag caatggagta ttcgtcagaa tggcaatctt agaagcagtg ctaaatggaa
    34381 aagcgtaatt ataggaggat gaaaaaatga aaacactcat taaaaacggt cagatcaata
    34441 cacgaaaaaa tatgacgaca ccagcagaaa tttggataga agatgggcga atcaaagcaa
    34501 tcggtacagg attctcagaa gccgaatttg atgaagtgtt tgatgcaaag ggacaattga
    34561 tcacacctgg tttagtggac gtccatgttc atttgagaga gccaggattt acatacaagg
    34621 agacgatcga agctgggact cgctcagctg ctcgtggcgg tttcactact gtatgcgcga
    34681 tgccgaattt aaatcctgta cctgatacgg cagaaaagct gagacaagta tatgacatca
    34741 ttagaaaaga tgcagttgtg aaggtattgc agtatgcgcc aatcacagaa aatctgcgaa
    34801 gtgagaaatt agttgatcaa gaagcactga ttgaagaagg tgcttttgct ttcacaaatg
    34861 acggtgtagg tgtccaaaca gctgggacga tgtatctggc tatgaaagaa gctgcaaaaa
    34921 acaataaagc cctagtagcc catacagaag atgaatcttt attatttggc ggcgttatgc
    34981 acgctggcaa gaaggcagaa gaacttggtc ttcccgggat cttaagcgta accgagtctt
    35041 ctcaaattgc tagagatctg ttgctagctg aagcaactgg tgttcattac catgtttgcc
    35101 atgtttctac taaagaaagt gtccgtgtaa tcagagacgc aaaaaaagca ggaatccacg
    35161 taacagcaga agtttcaccc catcatctta ttttgatcga cgaagatatt cccgaagatt
    35221 tcggcttttg gaaaatgaac ccgccgctaa gaggacggga ggacagagaa gctttgatcg
    35281 aaggcttgct tgacggtacg atcgattgca tcgctacaga ccatgccccc catggattag
    35341 aagaaaaaag ccaaagtttc atgaaatcac catttggtat cgtaggaagc gaaacagcgt
    35401 ttcaactgat ctatactcat tttgtagaaa ctggtcgatt cacgttggaa caagtgatca
    35461 attggttggc agttaaaccg gctgagattt ttggtttgaa tgcaggaacg ctaacggttg
    35521 gtgcccccgc tgatgtagca gtttttgata ttacacaaac ttgcacgatc gataaagaag
    35581 actttttatc aaaaggggag aatacacctt ttatcggctg gaaagtaaaa ggcgaaacac
    35641 aaatgacttt tgtcaacgga aaacttgtgt ggcagaaggg agaataaaag tatggaacgt
    35701 ttgcttatat tagaagatgg aacagttttc gaaggaaagg cttttggtgc agatgctagt
    35761 gtcttcggag aaatcgtttt tacaacaagt atgactggtt atcaagaaac tatcactgat
    35821 caaagtttta acggtcaaat cattacattt acctatccta tggtgggtaa ttatggggta
    35881 aatagagatg attatgaatc gatttctcca acatgcaaag gagtagtagt aaaagaacat
    35941 gcacgtgtag cttcaaactg gcgaaaccaa atgacgctag attcattttt gaaacaaaaa
    36001 ggaattcctg gaatatcagg tatcgatacg cgcgccctta ctcgcaaaat ccgtgaagtc
    36061 ggaacgatga aagctagtat cgttgatgct ggagattcct tcgaacatgc atttgatcag
    36121 ctgaaagcaa ccgttctagc aaccaatcaa gtgcagcaag tctcaactaa caaaccttat
    36181 ccaagtccag gaacaggtcg gaaagtcatt gtcattgact tcggcttgaa acatagtatt
    36241 ttacgagaat tgagtaaaag aaactgtaac ctcacagtac ttccctataa cacagatgcc
    36301 gagacgattt tagcgctatc accagacgga gtcatgctga cgaatggacc tggtgatcca
    36361 aaagatgtgc cagaagcaat cgaaatgatc aaaaaaatcc aaggaaaagt accgattttt
    36421 ggtatttgtc tcggtcacca gttatttgct ttagccaacg gtgcagatac ctataaaatg
    36481 aaattcggac atcgaggatt aaatcacccg gtacgagaag tagcgaccgg aagaattgat
    36541 tttacttctc aaaatcatgg ctatgcagtc gatgaagcca cgattgatcc agaaaaactc
    36601 atcgtaactc atgtagaagt aaatgacgga acagtagaag gagtcaaaca cagagattat
    36661 cccgctttca gtgtgcagtt tcatccagat gctgcccctg gtccgcatga tgcactacat
    36721 ttatttgatg aatttatgga aatgatggac gcaggaaagg agcagtaaac agatgccgaa
    36781 acgtacagat atcaagaaaa ttatggtgat tggatctggt ccgatcatca ttggtcaagc
    36841 cgcagaattt gactacgcag gaacacaagc ctgccttgcc ttaaaagaag aaggatatga
    36901 agtggtttta gtaaattcta atcctgcgac gatcatgacg gataaagaag tggcagatca
    36961 agtatatatc gaaccgatca cacttgaatt tgtatcaagg attcttcgaa aagagcgtcc
    37021 agatgctctc cttccaactt taggcggaca gacaggcctc aatatggcaa tggagctggc
    37081 agcatcaggt attttagaag aattgaacat agaacttcta ggaacaaagc tttctgcgat
    37141 cgatcaagca gaagatcgtg atctattcaa acagttgatg aaagaattga atcagccaat
    37201 ccccgagtct gaaatcgtca atacagtaga agaagctgta gattttgcaa ataaaatcgg
    37261 ctatcctgtc atcgttcgtc cagcctttac tttaggcgga actggtggag gtatgtgtga
    37321 aaatgaagaa gaattacgta tcgtatcaga aaatggatta agtctttccc ccgctaccca
    37381 atgtttgatt gaacgaagta ttgcaggttt taaagaaatt gaatatgaag tgatgagaga
    37441 ttcggctgat aatgcaatcg ttgtctgcaa tatggaaaat tttgacccgg tgggtatcca
    37501 tacaggtgat tctatcgtat ttgcccctag tcaaacattg tctgaccatg aatatcaaat
    37561 gcttcgtgat gcttctcttt cgattattcg agcacttaag atcgagggag gctgtaatgt
    37621 ccaactcgct cttgatccac atagttttaa ttattatgtg attgaagtca atccgcgtgt
    37681 ttctcgttca tctgcgttag caagtaaggc gactggctat ccgatcgcta agctagctgc
    37741 taaaatagcg cttggattaa cattagacga aatgaaaaat cctgttacag gaacgacttt
    37801 tgcagaattt gaacctgcat tagattatgt tgtagctaaa attcctcgct ggccgtttga
    37861 taagtttgaa aacggagaac ggcttttagg cacgcagatg aaagcaactg gtgaagtgat
    37921 ggctatcggt cggaatatcg aagagtcttt attgaaagct gtgcgttctt tagaaatcgg
    37981 tacacaccat atcgaacttc ctgagttggc agacatcacg gacgatgacc taatccaaaa
    38041 agtggttcga gcacaagatg atcgcctgtt ttatgtagct gaagctatta gaagagggta
    38101 caccatagaa gaattagcgg atttgacaaa aatcgattta ttctttttag ataaactgtt
    38161 gcatatcgta gaaatcgaac agcagttgaa acagcagccg ttggatgaca agatattgaa
    38221 aacagcgaaa caaaacggtt ttacagacaa aaaagtggcg gagctgtggg aaataaacga
    38281 agaagccgtt cgccagatga gaaaagaaaa tgatttgatt cctgtatata agatggttga
    38341 cacttgtgca gcggaattcg aatcaaaaac accttatttt tatagttcct atgaatatga
    38401 aaatgaaagt tcacgatcag aaaagccctc aattctagta ctgggatctg gtcctatccg
    38461 tatcggacaa ggggttgaat ttgactatgc gactgtgcat tctgtcaaag caatccagca
    38521 ggcaggatac gaagcaatca tcatgaacag caatcctgaa acagtttcga ctgacttttc
    38581 tatttcggat aaattatatt ttgagccatt gaccttcgaa gatgtgatga atgtgatcga
    38641 gctagaacag ccgataggag taatcgttca atttggtggt cagacagcaa tcaacttagc
    38701 cgaacctttg actgctgctg gtgtaaaaat cttagggaca acgatcgaag acttggatcg
    38761 tgcggaaaat cgtgatttat tcgagcaagc gctgaaagag ttggatattc ctcaaccttt
    38821 aggggatact gctacaagta aagaagaagc cgtggttatc gcttcgagaa ttggttatcc
    38881 agttcttgtt cgtccaagct atgtattagg cggacgtgcg atggaaatcg ttgaaaacca
    38941 gcgagattta gaagattaca tggaacatgc tgtgaaagca tctccagaac atccagtact
    39001 agtggaccgt tacttgcttg gtggtgaatg cgaagtggat gcgatttgtg acggtgaaac
    39061 agtactcatc ccagggatca tggagcatat tgagcgtgct ggtgtccatt caggggattc
    39121 aatggctgtg tatccacctc aaacattatc tgaatcgatc aagaaaacga tcgaagatta
    39181 cacgatccgc ttagcattag gtttgaattg tgtcgggatg atgaatatcc agtttgtcat
    39241 ccatgaaaat gaggtttatg tgatcgaagt caatcctcga gcaagtcgga ctgttccatt
    39301 cttaagtaag gtgacaggaa ttccaatggc acaaattgcg acgaaagcta ttttgggaga
    39361 gaaactaaag acacttggtt atgaaaacgg actttatcca gaaacaaaag aagttcatgt
    39421 taaagcacct gtcttttctt ttacgaaatt gcagaaagtt gatacttact tagggcctga
    39481 aatgaaatcg actggagaag taatgggatc tgacagacaa ttagacaaag ctttgtacaa
    39541 ggcattcgaa gcttctggat tacacttgcc tgaatatgga gccgtattat ttacgattgc
    39601 ggacgaaaca aaagaagaag cactagaatt agctaaacgt tttacagaaa ttggctatag
    39661 tctgatagga acgaaacata cagctgctta tttcgaaaaa aatggtctgg tcgttgaaac
    39721 agtagcgaaa atttctgagg aagcagcaga aaaaaatgta gtcgacttaa taagagatgg
    39781 taaaacacaa gtggttgtca atacgattga taaagatcgg ggaaatgctt ctaaagacgg
    39841 tttcatcatc cgaagagaag cagttgaaca tggtattcca ttgtttactt ctttagacac
    39901 agcagatgcg atcgttcgag tgatggaatc ccgtgctttt tcaactaacc ctatttaacg
    39961 gcgagcacat gttaagacaa aaagacgaaa aaccgttgca ggaggggaca gatgaaacaa
    40021 gaaatgatgg aaatcgttca gcaaaaacag ctggcaccaa agatttttga aatgacattg
    40081 aaaggtgatc tagtaaaaga aatgcagcaa ccaggacagt tcttgcatat tctcgtgccg
    40141 cgtgcagact tgctattacg ccgaccgatt agcttgaatc atatcgacca cgaaacaaat
    40201 acttgccgta tcatttatcg ggtggaaggt gaggggacta gagtcttctc tactctttct
    40261 gccggagacc atttagatgt catgggtcct ttaggcaatg gttttgattt gtccaatctt
    40321 catacaggag acgaagtatt tataatcggt ggaggaatcg gtgtccctcc tctatatgag
    40381 ttatcaaaac agttagtcga aaaaggaatt aagccaacgc attttttagg ttttgccaca
    40441 catgaagtcg tttattatga aaaagaattt aatcgtctag gagaaacaag aatcgctact
    40501 gacgatggaa catacggtat gcacggaaat gtcggtaatt tattgttagg aacgaagacg
    40561 gaacctacag ctgtttttgc atgtggttca aacgggttat tgaagacagt ggaacagctg
    40621 ttttcttcgc atcaaaatgt ccagttatca ttagaatccc gcatggcatg tgggataggg
    40681 gcttgctatg cttgcgtttg tcatacgccg gaagatgaaa caggaacaaa aagtgtgaaa
    40741 gtttgtgacg aaggaccgat ttttaaaata ggagaggtaa ttatatgaat gagaatcctt
    40801 tagcggtcaa acttcctggc ctagaattaa aaaatcctat catgccggca agcggctgtt
    40861 tcggttttgg caaagaatac ggaaaatact atgatttgaa tcagcttgga tcgatcatgg
    40921 tcaaggcaac gactccaaat gcccgctttg gcaatcccac gccaagagtg gcggaaacac
    40981 catctggcat gttgaatgca attggtttgc aaaatcctgg tctagacgtt gttatgcata
    41041 aacttcttcc agaattagag gagtacaatg agttacccat catcgcaaat gttgctggtg
    41101 cttgtgaaga agactatgta gaagtttgta gcaaaattgg cgaagcacca aatgtgaaag
    41161 caatcgagct gaacatttct tgcccaaatg tcaaacatgg cggaattgct tttggaacag
    41221 attctgatgt tgcttttcaa ttaacacaag cagtaaagaa agtttcttct gttcctgtct
    41281 atgtcaaatt gtccccaaat gtaacagaca tcgttccaat cgctcaggca atcgaagctg
    41341 gaggggcaga tgggtttacg atgatcaata ctcttcttgg tatgcggatt gaccttaaga
    41401 caagaaaacc gattttagcc aatcaaacag gtggactctc cggaccagca atcaagcctg
    41461 tcgctattcg tttgattcac caagtagcaa gtatatccag ccttccaatc attgggatgg
    41521 gcggcgtgca gacagtggat gatgttcttg agatgtttat ggctggagca agtgctgtag
    41581 ctatagggac cgcgaatttc accgatccgt atatctgtcc taaactaatt aaagagctcc
    41641 ctgtgcgaat gagcgaacta ggcatcgaat cgctggaaag attaatcaaa gaagtgagag
    41701 aggaacgtga acgatgaatc aactagacca acgaccaatt attgcattag atttttcaac
    41761 taggcaagag gtagaagact ttcttcgatt tttcccgaaa gaagaaaaat tgtttgtgaa
    41821 aatcggaatg gaattgttct accaagaagg acctgaaatc gtacgttatc taaaagaggc
    41881 tggtcataat gtatttttag atctgaaact tcacgatatt ccgaatacgg tcgaaaaagc
    41941 aatgcgggga ttggcaaagc ttggtgttga tctcacctgt gttcatgccg ctggcggtat
    42001 tcgtatgatg gaggcggcaa tgcgaggatt agaagagggg actccagaag gtggaaaacg
    42061 tccgctactt ctagctatca ctcagcttac ttctacaagt gaagaagaaa tgcatgccga
    42121 tcaactaatc gaagtttctt tagaaaaaag tgtgatccat tatgctagct gtgccaaaaa
    42181 agcaggatta gatggtgtgg tttcttctgc ttgggaagta gaagcgatca aagaaatggc
    42241 aggagacgaa tttgtttgct taactccggg tataagacca gaaggaacag tcgcaggaga
    42301 tcagacaaga gtagtcacac cttctcaggc aaaaaagatc ggctcaactt ttatcgttat
    42361 aggtcgacca atcactcagt caactaatcc ttataaagca tatcaaacga ttcaaacaga
    42421 atggagctaa cctaaaatga atgttgaaca aagtatcgca aaagacttat tagaaattga
    42481 agcagtattt ttaaatccaa gtaatccttt tacatgggca agtggaatca aaagcccaat
    42541 ttactgtgat aaccgaatca cgatgagcta tccaaaagtc cgaaaagaga tcgcaaaagg
    42601 attagcaagc aaaatcaaag aagcttaccc tgaagtacaa gtgattgctg gaactgcaac
    42661 agcaggaatc cctcatgccg catgggtagc ggaaatactg gatttgccaa tggtctatat
    42721 tcgaagtaag gcgaaagatc atgggaaagg taaccaaatc gaaggacgta tatttgaagg
    42781 tcagaagatg gttgtgatcg aggatttgat ttctacaggc ggaagtgtct tggaagcagc
    42841 tgaagcagca aaaagagaag gtgcggatat ccttggtgtt gcggctatct ttacgtatga
    42901 gttgccaaaa ggaaaagcaa atttcgaaaa agccgaaatc cctcttatga cgttgaccaa
    42961 ttattctgta ttgattgaag cagctttaga agatcgttat atcgatgagc aagaattaac
    43021 tctgctaaaa gaatggaaaa aagatcctga aaattggcag atttaagcgc gcttgatgaa
    43081 gatagagaaa tccgatttgt gtctccagaa tcatcaattg gaatagcaaa aatccaaaaa
    43141 ctgattgcga gaagcatctt ttcatcaggt atgaaaagaa acttctatcg tgatcagttt
    43201 ttgatttttt taaaattcat ttttctaaga ctggttagca gaaagaagag aataatctct
    43261 tctctttttt ctattatata tagattttct ctattttttg gtaagggagc tgtttcaaaa
    43321 atggtaaaga gcggaaatgt tgcgaatatc agttatttaa tgatatgata ttcggaaaaa
    43381 gaaatctcaa agggaggtaa cttttatggg gttgaatgaa tttattttag gattgaacaa
    43441 tttagagacg atcacaaggg cacctggatt ttttaaatat acagagcata cagttgcagc
    43501 acattcgtat cgtgtagcat ctattgcgca agttctagga gacattgaag aagcgaatgg
    43561 acaaccgatc gattggaaac tgctctatga aaaatcattg aatcatgact acacagagcg
    43621 ttttattggt gatatcaaaa cgccggtgaa atacgcaagc aaagagttaa gagggatgct
    43681 gcaaacagtt gaagaaaaga tgacggacga attcatcaac caagagattc cagctgagtt
    43741 ccaagaagtt tatcgccgtc ggttgttcga aggaaaagat gaaaccgtag aaggagaact
    43801 attagccatt tcagataagg ttgaccttct ttatgaatct tttgatgaaa tcatcaaaaa
    43861 taacccggaa aaagtttata aggagatgtt cattgaggca gtgactacga ttaaaagctt
    43921 tgaacatcgg ccttctgttc agtatttctt taaagagata tttccggaac tattgaacca
    43981 ggaattttat ggaaaagagg cgtttttaga aaccgtctca acgattgtaa agaaagaaga
    44041 aaaataaaaa tgatatgaga tgcaggggga agtatagttg aaaaagaata tggatgtcga
    44101 atggggctat tctggagaca aaggtccgga aaattgggcg agtttatgtg attggtttgc
    44161 gagaggtgcg gaatttccat tgcagtcgcc tattcatttg accaaggggg aagaaaccaa
    44221 aatagaagga gacacgcttt cctttcatta tcaaaagcaa cggtttactg acaaggaatt
    44281 caaaaacacg attcatttag taccttttga ccaactcagt tatgtagagt tcaagaaaga
    44341 acgctattat ttgacagata ttcatttcca cttgccttct gaacatatca tcaacggaaa
    44401 acaggaagat attgaatttc attttgttca tatgaacagc aaaaaagaga atctagttgt
    44461 gggtgtcatg taccagctaa tggtacagga aggttggctg tataatcaag aaaatgggga
    44521 aagttggaac ttagaaaaac atgagcattg ggtaaatccg gatgtgttct ttccggagca
    44581 aaaaagccat ttccattata ttggctcatt gacaactccg cctaccagcg ggccaatcca
    44641 atggtttgta atggatcatg taggaaaact gaaccaagag tttcttctcc ccttcgatgg
    44701 tcagtatgct cgaccgaaca accgaccaat ccagcccttg aatggaagag aaatttatta
    44761 tttcgaggag tttgagcaat gaatattcag cagatgaaat acgtggtcgc tgtagcaaac
    44821 aatggtagtt tcagagaagc ggcaaagaaa atgtttgtct cccagccaag tctttcagct
    44881 ggtatcaaag aattagaaac cgagttaggg atcacgctgt ttactcgaac gaatcgaggg
    44941 gcatatttaa cagaagaagg gcaagacttc ttaaatcgtg ccgagaaaat attggtccag
    45001 cttgatacat tagaaactca ctatctaacg agtgaaaaga gagacaattt ttcgatcgct
    45061 gcccagcatt atgatttctt aggtcctttg accgcacagc tactcgagga attcaaagac
    45121 gagatcaaac aatttcggat catggagacg acaacagcga gagtgatcga agaagtcaaa
    45181 gagcagcaca gtgagatcgg cattatttat atgaatgagc ataatcaagc tggtatcaga
    45241 agatatttac aacaaggaga gctaagtggc cgcagcattg gaaaattcaa aacgcatatt
    45301 tttttaaggc aaggacatcc attagctgaa cacgcagaga tcaccaaaga agaattgtta
    45361 tcttatcctc aagttcgttt tacacaggat ggaaataatt tcccgtattt ttatgaagac
    45421 cttatcgaga tccctgatca agaaacagtg atttatacga gtgatcgagg tactttgatg
    45481 aatatcgttc ttgaaactga tgcttacgct tcaggatctg gtattgtgat tggagggatt
    45541 cgcgagcact tgcggttaat ccctttggca gaaggcgaac taaatgaatt ttatatcatt
    45601 tattcggcga aacgtccgct cagtgaaatc gctcaaagat ttatcaaaga gcttactcgg
    45661 ttactggacc agcagagcta agaaaagcag atgagaacgc tgtttattga acaccgttga
    45721 tcaaaaaaga ggggttgtga cagaatttt
//
LOCUS       DACDYJ010000018        43753 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDYJ010000018 DACDYJ010000000
VERSION     DACDYJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.018"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(53..787)
                     /locus_tag="IWM51_000970"
     CDS             complement(53..787)
                     /locus_tag="IWM51_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9970405.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="IWM51_000971"
     CDS             1114..1524
                     /locus_tag="IWM51_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP9970406.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="IWM51_000972"
     CDS             1517..2218
                     /locus_tag="IWM51_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP9970407.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="IWM51_000973"
     CDS             2218..3804
                     /locus_tag="IWM51_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9970408.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="IWM51_000974"
     CDS             3823..4044
                     /locus_tag="IWM51_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP9970409.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="IWM51_000975"
     CDS             4041..4802
                     /locus_tag="IWM51_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP9970410.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="IWM51_000976"
     CDS             5182..5691
                     /locus_tag="IWM51_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9970411.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="IWM51_000977"
     CDS             complement(5766..6305)
                     /locus_tag="IWM51_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP9970412.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IWM51_000978"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IWM51_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP9970413.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7372..8169
                     /locus_tag="IWM51_000979"
     CDS             7372..8169
                     /locus_tag="IWM51_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP9970414.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8197..8832
                     /locus_tag="IWM51_000980"
     CDS             8197..8832
                     /locus_tag="IWM51_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP9970415.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="IWM51_000981"
     CDS             8852..9625
                     /locus_tag="IWM51_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9970416.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="IWM51_000982"
     CDS             10033..10830
                     /locus_tag="IWM51_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP9970417.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="IWM51_000983"
     CDS             10808..11221
                     /locus_tag="IWM51_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970418.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="IWM51_000984"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="IWM51_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9970419.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISSVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="IWM51_000985"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="IWM51_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP9970420.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="IWM51_000986"
     CDS             15998..16558
                     /locus_tag="IWM51_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP9970421.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IWM51_000987"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IWM51_000987"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP9970422.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="IWM51_000988"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="IWM51_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP9970423.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="IWM51_000989"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="IWM51_000989"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP9970424.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATEQPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="IWM51_000990"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="IWM51_000990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP9970425.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="IWM51_000991"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="IWM51_000991"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP9970426.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="IWM51_000992"
     CDS             21905..22483
                     /locus_tag="IWM51_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9970427.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="IWM51_000993"
     CDS             22535..23017
                     /locus_tag="IWM51_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP9970428.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="IWM51_000994"
     CDS             23134..23508
                     /locus_tag="IWM51_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970429.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24186..25538)
                     /gene="brnQ"
                     /locus_tag="IWM51_000995"
     CDS             complement(24186..25538)
                     /gene="brnQ"
                     /locus_tag="IWM51_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9970430.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25685..26275
                     /locus_tag="IWM51_000996"
     CDS             25685..26275
                     /locus_tag="IWM51_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9970431.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26403..27752)
                     /locus_tag="IWM51_000997"
     CDS             complement(26403..27752)
                     /locus_tag="IWM51_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9970432.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27920..28660)
                     /locus_tag="IWM51_000998"
     CDS             complement(27920..28660)
                     /locus_tag="IWM51_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9970433.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28673..30265)
                     /locus_tag="IWM51_000999"
     CDS             complement(28673..30265)
                     /locus_tag="IWM51_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9970434.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(30833..31129)
                     /locus_tag="IWM51_001000"
     CDS             complement(30833..31129)
                     /locus_tag="IWM51_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970435.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31305..32030
                     /locus_tag="IWM51_001001"
     CDS             31305..32030
                     /locus_tag="IWM51_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP9970436.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32023..32865
                     /locus_tag="IWM51_001002"
     CDS             32023..32865
                     /locus_tag="IWM51_001002"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP9970437.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32868..33389
                     /locus_tag="IWM51_001003"
     CDS             32868..33389
                     /locus_tag="IWM51_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP9970438.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33642..34205)
                     /locus_tag="IWM51_001004"
     CDS             complement(33642..34205)
                     /locus_tag="IWM51_001004"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9970439.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34456..34725
                     /gene="rpsO"
                     /locus_tag="IWM51_001005"
     CDS             34456..34725
                     /gene="rpsO"
                     /locus_tag="IWM51_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP9970440.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34913..37039
                     /gene="pnp"
                     /locus_tag="IWM51_001006"
     CDS             34913..37039
                     /gene="pnp"
                     /locus_tag="IWM51_001006"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP9970441.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37549..37815
                     /locus_tag="IWM51_001007"
     CDS             37549..37815
                     /locus_tag="IWM51_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9970442.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            37959..39950
                     /locus_tag="IWM51_001008"
     CDS             37959..39950
                     /locus_tag="IWM51_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP9970443.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40304..41605
                     /locus_tag="IWM51_001009"
     CDS             40304..41605
                     /locus_tag="IWM51_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9970444.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41735..43105)
                     /locus_tag="IWM51_001010"
     CDS             complement(41735..43105)
                     /locus_tag="IWM51_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP9970445.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43287..43610)
                     /locus_tag="IWM51_001011"
     CDS             complement(43287..43610)
                     /locus_tag="IWM51_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9970446.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
BASE COUNT        14163 a         8127 c         9112 g        12351 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agttcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcacccggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacaaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaatgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcaataga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgac gaaaataaaa taacaaacaa tttgtaaagt tgacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa aatttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcgaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacgattat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcacagcatg acagcattgg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ctagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttttt ttgcgaaaac tatttttata tcatatatat gtctaaaatc tatattataa
    23881 aagaaatgtt gtttgatcag cttctcttcg gtattagttc aaaacaaaac ggcaaagtat
    23941 atggagctac tttgccgttt tgtttctcta atactcaggt cagtttatag acgctcaata
    24001 aacggtgttc aaaagccagt ttctcggtat taagtcaaat ttatgcaaaa atgtgagaag
    24061 ccattttcaa aaaatatttc ttaatactcg aaactgagcg gctttttcac gacctctcaa
    24121 taaacagtgt tcaagagaca gcctatcggc aataaggctg aacgtctctt tcacaacctc
    24181 ttttatcaat ctgctacttc ttctttctta gcgaaactcc aaatcagtcc gataacaaaa
    24241 ccgataactg ctggtacgat ccaccccatc cccaatttga aaaacggcaa gtagctttcc
    24301 gcaacatata aaagattttg aacaataggt gtttgtttga tagtttctgg gcaagcgttc
    24361 aacccatcaa aaatcgaagc aatcaatgta aaataagtag tcattcgata aacagccgta
    24421 cgctgcttaa acaaaggacc aactaacact agtaaaatca gtgtcattgc taatggatag
    24481 atgaacatca aaacaggcaa cgaaaattga atgatattcg tcaagcctac gtttgccgct
    24541 aaacaaggaa ggatactagc aagtgcaaca aagaatgcgt aatttctttg cggaaacaaa
    24601 tctgtgaatg tttctgcaaa agctgtaatc aaaccaatac cggtcttcaa gcaagctaat
    24661 ataacgataa atgctaataa gatactgcca taagtaccta aataatgatc agcaatctga
    24721 gccagcgtga tcccgccatt ttcacttaga gcaaagctac ctaagctcat agcacctaaa
    24781 taagcgagca aagtataaat gattcccatc aatataatgc tgatcgcacc agatttgatc
    24841 gtgtctttag cgatttcagc aggttttgtt acacccatgt tttggattgt tgtgacaatg
    24901 atgatcccaa atgctaaagc agcaagcgca tctaatgtgt tatatccttg agtaaaccct
    24961 gtgaaaaaag catgctcttg ataatttggc tgaataggcg cttgatttac agaacctagt
    25021 ggattcaaaa aagcaaggag aagcaaagca cctaataaaa ccaaaaatgc aggattcagg
    25081 aactttccaa cataatctaa tatctttgtc ggttttcttg aaaaccacca agccagcaca
    25141 aaaaatacaa tactgaaaat tgctagatat agcgtctgtc tgctaggatc gataaatggt
    25201 gctaaaccaa tctcgaaaga tgtggttgca aggcgaggta aagcaaaaaa cggtccgata
    25261 actaagtaaa gcaagatggt aaaaaccatc gcatatgttt gatttatccg ttttgctaat
    25321 tcatatacac ctgagctttt cgatacacca atggcaatca cccctaagaa gggaagaccg
    25381 ataccggtaa ccaagaaacc tagattcgcc caaaaggtag cagcgcctgc ttcttgaccc
    25441 aaatgaacgg gaaagataag atttccagca ccaaaaaata gaccgaaaag catggagcct
    25501 ataaaaagat agtctttaaa tgataatttt tttgtcatac ataactcctt tacggttttt
    25561 ttaaaagtaa caaaatttcg aaaaatatgc aacaattttc agaaaatttc ctatataaaa
    25621 cgacggttat ttaataacgt aagtgataaa ataaaagcgc tgaatcatct gaggaggaat
    25681 catcgtgaca aagtcgagat tagaagcatt tactgatgga gtggtagcca ttgttttgag
    25741 agttcttgta ttagagatca agatccctaa tacatctagc tttcattctt tatgggtcat
    25801 ccgaaataca ttattagctt ataccatcag ctttatattt gttgcggtga tctgggtgaa
    25861 ccatcatcgt atatttcaga tggcagaacg aattaattac cgagtggtct ggtcgaatat
    25921 ctttttgctc ttttggctga ctttatgtcc ttctgttact agttgggtcg ggcgtaaccc
    25981 tgaagctttt tggcctgaga tggcttatgt cttagtatac acgatgtgga gtttttcttt
    26041 tggtatcttg atgaggcaaa tcatgaaagc aaataaacca gattcccatg tcgtgagagt
    26101 tttgtcaaag gaccgtcgaa gtcttatctc catgatgatt aacttatgct tgattgccgg
    26161 tgtctttttt gtaccgttga tcgggttatt cggacgtttt tttgtatctg gtatttggat
    26221 tttttcctat aaaaaagcag atgcctatta tcagcggttg tttccgggga aatagaaaag
    26281 aggctgggac agaagctttt aactccaaga aataagccga aatttcacaa aaatttaaga
    26341 agcaattttc gtaaattcct tcttatttct cgaagctgac cacttctgtc acaactactt
    26401 tcttacttct tacgttcata tttcatatga tagattagta atagaagcaa gaaccacaca
    26461 ggtgttagta ataaagcgat tctcgtatct tttcctagga acaagacgac acacacaaag
    26521 gccatgtaaa gaaataccag ataattacta aatgggtaca acggcatttt aaatgaatga
    26581 gcttttttgc cgaggatact tttacgatat ttcatgtgcg tgtagacaag catactccag
    26641 ataaacaaga aacaagtagt ggcaatactg cttactagtg tgaatacttc actaggcatc
    26701 acgtaattca agataacaga aatgaaaatg acgatagtag aaaatgtcaa agcattccct
    26761 ggcacatgat gtgttgttaa ctttttgaat tttttagggg cactgccttc ttgcgctaaa
    26821 gaacgaagca tacgtcctgt actatatatt gcactgttgc aggcagaagc tgctgcactt
    26881 aatacgacta aattgatcaa ggaagcagca atcgtgatcc caatttccga aaatacttct
    26941 acaaatggac tgttatctgg attcaagctg ttccatggat agattgccat aatgacaaaa
    27001 agagatccta agtaaaataa gatgatcctg ataggaatat tattgatagc ttccggcaac
    27061 actttttcag gattttctgt ttctccagcg actaatccaa ctaattctat cccagcaaaa
    27121 gcaaaggtta ccatttggaa agctaagata aatccttttg caccagttgg aaagaatccg
    27181 ccatgagacc atagattcgt gatactggct gtgccaatat ctgtcgtata gtgggtgagg
    27241 atcatataag ctcctacgag gataaaggca atgatcgcta caactttgat caatgcaaac
    27301 caaaactcta actcaccaaa caatgagaca gccaccatat tcaagcaaag aagaagacct
    27361 aatgcaatca gttcaggaag ccactgagga acaccaggcg cccagtagcg tacatacatt
    27421 ccgatggctg ttaaatcggc cattgcaatc gatacccagc agaaccaata cgtccagcca
    27481 gtgatgaaag ccgctttttt gccaagatat tctgcaacaa aatccagaaa agaatggtta
    27541 ttggtgtttg ataacagaag ttcgcctaac gcacgcataa tgaaaaaaca gatgcatcca
    27601 gtaataagat aggcgagcaa tatcgaaggg ccagctagct ggatagactt tccggaacct
    27661 aaaaacaacc ccgttccaat cgctccgcca atagaaatca gttggacatg tctatttttc
    27721 agtccacgct gcagttcttg ctcttcattc atttttaaat catcctaact aaaaaagttt
    27781 aattacttcc atattaaaag agttttctga aaaacacaac taagattcca aaattttcag
    27841 aaatttacct tttatgagta attcataaaa aagaactgcg gtaaacaccg cagctctttg
    27901 tcccaatcag ctattgatcc taagcattta aaatacgtga aaggaattct tttgtacgtt
    27961 cttctttcgg atggacaaag atatcttctg gtgtaccttc ttctgcaatc acacctttat
    28021 ccataaaaat gacacggtca gatacatctt ttgcaaattc catctcatgc gtcacgataa
    28081 gcattgtcaa gccagtatgt gctaagtctt tgatcgtatt aagcacttcg ccaaccattt
    28141 cagggtctaa cgccgaagta ggttcgtcga atagcaatac atctggattc attgataatg
    28201 cccgagcaat tgcgacacgc tgtttctgtc caccagataa ttgtgctggt ttggcttcga
    28261 tataacgatc cataccaact ttttccagat tttcgattgc tacttttttg gcagtttctt
    28321 tatctcgttt caggacagtt gtttgaccag acatacagtt ttccaagaca ttcatattgt
    28381 tgaacaggtt gaaggattgg aaaaccatac ctagattcgt tcgatattta ggtaaattat
    28441 atttaggagc taatacattt tctccattat aaatgatctc gccgcctgtt ggtttttcca
    28501 gtaaattgat acagcgcaag aaagtagatt taccagaacc ggaagaaccg atgatcgtca
    28561 caacttcacc tttattgact gttacgctga tatctttcaa tacttcattt tcaccgaagc
    28621 tttttcttag atgtttgatt tcaataattt tttcattcat gattcttcct ccttattcct
    28681 gcaaatttga attgtcgatt tcttcaagct tggtataagc tgaaggacca tcgattttct
    28741 tttctaccgc ccgcaggatt ctggtgatcg taaaggtcat gactaagtag atgatcccga
    28801 cgatcgtaaa tgtttggaag aattggaaat tcgcaccgga agcagagttt ccttggaaga
    28861 acaaatcagc tacgccgatt acacttaaaa ctgctgtatc tttgatattg ataacaaact
    28921 catttcctgt tgctggcaag atattccgta aaacttgtgg aataacaact tttctcatcg
    28981 tttggccatg ggtcatacca atggcttgcg cagcttcgaa ctgcccttca tctactgcaa
    29041 aaatcccccc acgaacgatc tctgtcatgt aagctccagt attgagagag acaataaaca
    29101 aagcggcaat cgttcggttc aatgaaatgc caaaagcgag tgccagaccg tagaagatga
    29161 ccattgcttg taccatcatc ggtgttccgc ggaatacttc gacatagata gaaagaagtg
    29221 catttccgat tttttggaag aagcgagtca gtgcatttcc tgattcaggt accgaacgga
    29281 agacaccaat cagtaatcca agtgtagtac cgacaactgt cccgatcaat gcgatgaaca
    29341 aggtaagtcc agcacctcgt aagaacatat tgccgtattg tttccaaatc gtagtgaaat
    29401 cagaaatgat gccaggtttt tcttcactat cagttgcagc aggctgatct ttgactgctt
    29461 ggtccataat tttggttcgt tcatcgttag agatgccaga aagaatctgg tttacttttt
    29521 ggatatccgg gtcgccttta cgcataccaa cagaaatctg aacatcttcc gggtttgtct
    29581 ggaagccatc tgcaccgctg aattcaagca tttccaagtc tttgttcaca ctagttgccg
    29641 ttacaccttc cggacgttcg ctgacataac catcgatcat tccagaagct aaagctgttc
    29701 tcattgctga gaaattatcc attgcttgtt gtttctgcac atctggtatc tgatcgatca
    29761 cagtatagtg gaatgtattt aactgagcag taatctttgc tccagaaaga tctgctaaac
    29821 tttttgcttt tgcatatttg ctatcttttt gaactactac tactaattgt gattcgtaat
    29881 aaggatcggt aaaatcaatt tcttttcgtc gttcagcagt agggctcatt ccggcgatga
    29941 tcgcgtcgat cttgcctgat tgaagggcag gagccagacc atcccatttc gtttggacga
    30001 tgactaattt tttccctaaa ccatctgcta cttttttagc gatttgaaca tcataaccgc
    30061 cggcgtaact attttggcct tgaattggca aggcattatt ctcatctgtt tgttgagacc
    30121 agttgaaggg tgcataccca gcctccatac ctacgcgaaa ctcatcgctc ggtgtttttt
    30181 catctgcatt tgccagagat cccacactga atagtgagaa gacaaaagca aatagtacgg
    30241 caactgaaac catatttttt ttcatttttt tctccctttc tgattatatg atacacgagc
    30301 ggtctagatg aaatgcggta aagctttatc tgaaaaaata aaaagcgacc gatttataga
    30361 tataaaaagg ccgctaaaat tccataaggt gataaacctc tcacaaaaca tagcgcaact
    30421 tagcatcact gctaagacag tccgcaagct cttaacttgc gtcccaacat acactccaag
    30481 caaaaaagtg cacatttcgg cgatcctccc tattcctgct tctccatgtt tgcttcactc
    30541 cacagggtta atgattctcg cgacctctac ctcattgatt gaggcatctc gtattcagtt
    30601 atttatacaa aaaaagttta cgatactcga gaggctttgt caatctataa ctgctgtttt
    30661 ttgaaaaaat actgataaat atagaaatct ttacttttac cgaaaaaaca atcaaaatgt
    30721 gaatcatcta tccattgcaa acgatagtat aattatacac aaaaatgtat gtaatacaat
    30781 aaaaaaagaa ctagaacaga aaatttcctg tgtccagttc catctcgatc atttaagctg
    30841 ccgctttctt tggctcacgt cctaggatcg cttgcaaaag aacaacgacg ccataaatgg
    30901 ctaatattag ataaatcaaa cttgagtatt gggtgattcc ccagccccat gttcgatata
    30961 cggctaatgt aatggctaaa acaattgcgg caatcacgtt gaataaagca aaagcccaaa
    31021 gatttccatt ttctttgcgt acaagataaa aaacagagaa ataaacgctt aacgctaacc
    31081 aggcgataca agccagaata acgccccaat aaataaagaa tggtaacata tcgattcacc
    31141 tcacttttat acagcagtat tgtaacacgt tttcattctt ttgtcagtga acattgtgat
    31201 aaattgttca aatgcccata acactttttt attcccattt ttctgacttt tcaacaataa
    31261 atttgttcga gtatgggcta taatggaaag tgaggtgaca aaacatgaaa caattaacag
    31321 tagacgaagc agcaaaagag atattagctt atcagcaaat tacatttttg accggtgcag
    31381 gggtatccac accttctgga gtgccagact atcgatcttt gtcaggaatc taccacggga
    31441 tggagcagcc ggaatacttg ctgagtcatc aagcaatggt gaatgaaccg gataaattct
    31501 atcaatttgt caaaaagatt tatcatcctg aagcaaaacc caatatcatt catttggaaa
    31561 tggcacatct agcgaaagaa aaaaatgttt gggttgtttc ccaaaacatc gacggtttgc
    31621 acgcaaaggc taaaagcccg caactcgtca attttcatgg aagtttgtat caatgtcatt
    31681 gtcgaaaatg tcaacaaacg gtagactgga aagaatatct tcacagcgac aaacatcaag
    31741 catgcggagg acaaatacgc ccagacatcg tgttatatgg tgaagggttt caagatggag
    31801 tcatggagca ggcggcttat gccgtcagcc aagcagagct gatcgtcatt gttggaacga
    31861 gttttcaagt tcatccgttt tgtgatctgg tacagtttcg ccagccgtca gccaaaatct
    31921 tagtaatcaa tcaaacgcct gtttatttag cggagcctta tacgtttatt caagaagacg
    31981 gagcggttgt tttcaaaaaa atacaagaat taggagcaga ttatgactga attcaaaacc
    32041 gaaaaagaaa aaatgattgc aggcgagctt tattttgcca atgatcctga gctggttgcc
    32101 gaccgtatgt ttgcaagaag tcaaagccaa atcatcaacc aagcagaatc agcggaactg
    32161 cgatcccaat tgttgaaaga gacttttggc cgtacaggga aaaaaatcta tatggagcca
    32221 gttatcaatt ttgattacgg atataatata tttgttgggg agaattttta tgcaaatttc
    32281 aattgtacat ttttagatgt cagcacgatt gaaatcggtg ataactgcat gttcgctccg
    32341 aatgtccagc tttacacggc gacgcatcca ctacatccag tgaaacgtaa cagcggtctg
    32401 gagtacgcaa aacccattaa aatcggtaat aatgtttggt taggcggagg agttatcgtc
    32461 acgccgggtg tcactctagg ggacaacgta gtagtcggcg caggttcggt tgtgaccaaa
    32521 tcttttcccg acaatgtagt gattgcagga aatcctgctc gtatcatcaa gacagtagag
    32581 gaagaactaa cagaagagtc tcttgaaaca ttacgtcata agatcgatat gatcgatcga
    32641 gagttgattg ctttattaga aaaaagaatg gatacggtaa ctaaaatagg acaagtgaaa
    32701 aaagaagaaa accaagcagt ttatgatgca aaaagagagc agcaagtatt ggataaagtt
    32761 gttagtctgc tgaaaaataa gggatataag gaaacaataa cagatacgta tgttgatttg
    32821 atgaaacact cgcgcgaata ccaaaacaaa atgaaggagg aataagtgtg atcgaaggag
    32881 agacacggcg aaaacaaatc atgcaaacct tggcaaatac agaaaaacca gtgagtgcta
    32941 gcaagtttgc gaaaactttt ggagtcagcc gtcagatcat tgtaggcgat attgctttac
    33001 tacgagctgc aggtaaaagt atcgtggcaa cagcaagagg ttatatcctc gaaacagaag
    33061 aaaccaatac tgggcacatc agtaaaatcg ctgtccagca tggaaaagat caaacagaag
    33121 aagaactgag actaatcgtt gaaaatggtg gtgaaatcat tgatgtaatt gtggaacatc
    33181 ccctttacgg tgagttagca ggaactttgc acatcaaaac gccacaagat atcgattcct
    33241 ttatgagacg ttatcaaaaa agtaatgcta ctttgttatc cgaattaaca ggcggcatcc
    33301 atctgcatac gatccgctac ccagaaaagc atatgctgaa aaacatcaaa caaaaactag
    33361 ctgaagcagg aatcctctac gaaggataac atcgtagctg ctacttttta tcctagaaac
    33421 tattctatca tctctgagat aaacacccat atggaagaaa taagaaaaaa taagaaaaga
    33481 tgcgtatcca atacacagaa ttttttggaa ttgcgatttc tagatgtcta tctaaatttc
    33541 gattagtgga gaaacataaa aaagggaagt gcagcttttg agcactcccc ttttttataa
    33601 agctttcttg tagcatagta tcgaaggaac ctgccaattc tctactcgat caccaatacg
    33661 ccttctttta gtgcaaatgg attttgatcg ttgatgtggt catagaacat cacaccgttg
    33721 atatggtcga tttcatgttg aacgacgatc gattcataat tcttcaaacg aatcttgtgt
    33781 ttttcaccat tcatatcata ataagacaca gtgattttgg catggcgaac aacatatcct
    33841 ggtacttctc gatcaacaga taaacagcct tcaccttccc ctaaacaagc atcttgaaca
    33901 gaatggctca aaattttagg attatacata acagtactta gagaaggtcc atctgcctct
    33961 gggtcaggac ttggtacgtg aacagcaatg attcttttag aaatatctaa ttgaggagca
    34021 gctaagccaa caccgcctcg taagtgtagt tcttcagctt tgattggatc ttggctgttt
    34081 tttaagaact caagcatctc ttttcctaaa gagatatctt cttcgcttaa tggaagagaa
    34141 acttcttttg caacttcgcg taaggtcgga tttccttcac gaatgatatc atccattgta
    34201 atcatggaat atgtacccct ttcttaaaag actgtgtttc aagtctattt tcgcattata
    34261 gtttaccaca aaaatggaaa agtagtagta agattgtttg aaaactacct tttcttaatg
    34321 attaactact tgattttcaa cccggtttca ggtaaagtat agctgtgtgc tctaggcaca
    34381 tgacctgcca cttggttaag cgaatcacca acgttttact cagtcgcagg ggaataatcg
    34441 aaagaggtga aagaaatggc aatttcaaaa gaacgcaaga atgaaatcat caacgaatac
    34501 gctcgccatg aaggggatac tggttctcca gaagtacaaa tcgctgtatt aactgaagaa
    34561 atcaaccact tgaatgaaca tgcacgcgtg cacaaaaaag accatcattc ataccgcggc
    34621 ctaatgaaaa aagttggtca ccgccgtaac ttgttagctt acttacgtaa aactgacgtt
    34681 caacgttacc gcgaattgat caaacgttta ggcttgcgtc gttaatttta gtcaaaaaga
    34741 gtgggattca catgaatctc acttttttta ctcgtatatc aattaagtaa aattccgtga
    34801 tcaagctaga tggactccta ctaaccaaat gaaagctgct caaaagacgg ctttcggcag
    34861 ttttcatttg tttagtagcg tcttgctttc acgagaagga aaaggagaag ttatgtcaga
    34921 aaaacaagta ttcaaaacga catggggcgg ccgtccctta caaatcgaag tcggtcagct
    34981 agcaaaacaa gcaaacggag ccgttttagt ccgttatgga gatacagtag tcttaagtgc
    35041 agctgtggcc tctaaagaag caaaagatac agactttttc ccattaacga tcaattatga
    35101 agaaaaaatg tatgcagcag ggaaaatccc tggaggtttc atcaaacgag aaggccgtcc
    35161 aagtacggaa gcaacattga ctgcccgttt gatcgaccgt ccaattcgcc cgatgtttgc
    35221 agaaggcttc cgtaatgaag tccaagtaac gaacatcgtg atgagtgtag aaacagactg
    35281 ctcgccagct atggcagcaa tgctaggttc ttctttagca ctatcaatct cagatatccc
    35341 atttgacggc ccaatcgcag gcgtagaagt tggtcgtgta aatggcgagt acgtcttgaa
    35401 tccaacagta gaacaagcag aacaaacaga tatcgaattg actgttgccg gaacgaaaca
    35461 agccatcaat atggttgaaa gcggcgcgaa agaagtttca gaagaagaca tgcttggtgc
    35521 attgctgttt ggttttgacg ctatcaaaga gttagttgct ttccaagaag aaatcgttca
    35581 agcagtaggg aaagaaaaaa tggaagtgac tttactgcaa gttgatgaag tattgaaaaa
    35641 agaaatcttt gatgcttctt acgctacaat gaaagcagca gtaatgactg aagaaaaact
    35701 tgctcgcgaa gataatatcg agcaggtgaa gatcgatatc cgcgaagctt atgctgagaa
    35761 atttgcaggt catgaagatg aagatcactt gttgaaagaa gttaaacaaa tcacagaaga
    35821 tcttgaaaaa gatgtcgtac gtgaattgat caccatcgat aagatccgtc ctgacgggcg
    35881 taaattggat gaaatccgtc cattgtcatc agaggtcagc ttattgccac gtgtacatgg
    35941 atcaggtttg ttcacacgag gacaaacaca agctttatca gcttgtacac tcgcacctct
    36001 aggagagcac caaatcattg atggtttagg ggtggaagtc agcaaacgat tcatccacca
    36061 ctacaatttt cctcaatttt ctgttggttc tacaggacgt gcaggctctc cagggcgtcg
    36121 ggaaatcgga catggcgcat taggtgaacg tgcgttagca caagtaatcc cttcagaaga
    36181 agaatttcct tatacgatcc gtttagtagc agaagtattg gaatcaaatg gttcttcttc
    36241 ccaagcaagt atctgtgctg gcacattagc attgatggat gcaggtgtac caatcaaagc
    36301 gccagtcgcc ggtattgcga tgggacttgt ttctgatggt gaaaactata cgatcctaac
    36361 tgatatccaa gggctggaag atcatcttgg cgatatggac tttaaagttg ccggtacaaa
    36421 agatggtatc actgctttac agatggatat caagattcaa ggaatcacag aacaaatcct
    36481 aactgaagca ttaactcaag caaaacaagc acgtatggaa atcttagaag aattgacttc
    36541 aacgattgct gctccacgtg aagaattaag ccaatatgca ccaaaaatcg aaatgatcca
    36601 gatcgaacct gctaaaatca aagatgttat cggtaaaggc ggcgatacga tcaacggaat
    36661 catcgatgaa actggtgtga aaatcgatat cgatcaagat ggaaaagtaa gtattgcttc
    36721 agctgatgca gaaatgatca aaaaagctat caagatcatc gaagatttga ccaaagaagt
    36781 caaagttggt gaagtttatc tagggaaagt tgtccgtatc gaaaaattcg gtgcattcgt
    36841 caacttgatc aaaggaaaag atggacttgt ccatatttcc caattagcaa acgatcgtgt
    36901 caataaagtc gaagatgttg ttaaactagg tgatgagatc cttgtgaaag taactgaaat
    36961 cgataaacaa ggacgcgtaa atctgtcaag aaaagcaatg ttgaacgaag atggttcaac
    37021 gaaaaatgaa gaaaaataat ttaattcata ggaataatgt acttgtgatc gtgaaggtga
    37081 cagggggaca ataattattc gaaaaaaggg gactgtgaca aaagccgtgt cacagtcctt
    37141 ttttttcgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc agccaagtta
    37201 aaaacattag gagctgtttt tatttggctg ttcttattac ttgatgcaga acagcttttt
    37261 tacaacctct ccaataaacg atgttcaatc agctgtccct cgatattagc tcaaaaaacg
    37321 gcaaaatatg aggaactatt tttccgtttg tttctctaat actcaggtca aaccgctgat
    37381 ttcacaacct ctgattcacg gtgttccaga agcagcttct acggaaataa gctgaacact
    37441 tttgttacaa cttctttttt tctggagttt cttggcgcat atttttggag tccttctgaa
    37501 taaagcgtaa aataaaagtg agaaatatgg ataaaggagg gaaaggagat gctgaatttt
    37561 ttatggtcgt tatttatcgg cggtatatta ggtttgatag ctggcgcaat tttaggaaag
    37621 gaagtcccgg gaggcgtatc tggaaatatc attatcggtt ttttaggtag ttggctcggc
    37681 gaatttttac taggaccact gggaccggaa ctcggcggtt tttatttgat accagcatta
    37741 ttgggtgcaa ttttttgcct tgctctttat tcatttgtgg caagacgtat cagagaacgt
    37801 ccaaagaaag aataatttta ttcaaataag ttgacacttc agtgtgttac ttggataatt
    37861 agaagcaatt taggaaagcg ggtgtcatgg ataagaatat catctatcat tcaagcttac
    37921 ctaaaggata tccatataac aagaaatgag ggaaaaacgt ggcaacaacg cacaatacca
    37981 aaaagatatc aatggctggc ctgcttgtag cgatgggagt cgtctacgga gacatcggga
    38041 caagtccgct ttacgttatg aaatcgattg ttgaagataa tggcggattg tcaggaatca
    38101 atcctgattt tgttatcgga tcagtctcat tgattttttg gacattgact ctactgacaa
    38161 caatcaaata tgtcgtgatt gcattgaatg cagataacca tggtgaaggt ggaattttct
    38221 cactctacac acttgtccga aaaggtggga aatatttgat cattcctgca atgatcggag
    38281 gagctgcctt actagcagat ggggtattga cccctgctgt gacggtaacg actgcgattg
    38341 aaggcttgcg aggaattccg cagttttttg accggttcgg taataaccag aacgtgatcg
    38401 tcattatcac tttagttatt atttttgttt tattcatggt ccaacgcttt ggtacagata
    38461 ttgtcggaaa agcattcgga ccaatcatgt tcgcttggtt tactttttta ggcgtaatgg
    38521 gattaatgaa cttcagtcaa gactggacag ttatccgtgc actgaatcct tattatgcta
    38581 tccagctgct ttttagtgca gataacaaat taggcttgtt cattcttgga aatattttcc
    38641 tggctacgac aggtgcagag gcgctttatt ccgatcttgg acatgtcggc aaacgaaata
    38701 ttcgtctgag ctggccttac gtcaaaattt gtttggttat caattatttt gggcaggctg
    38761 cgtggttgtt gaatgtttat caaaaccctg aagcacagca aatcaagaac ctgaatccat
    38821 tcttccaaat gatgcctcaa agttggatcg tgatcggcgt tgcttttgca acagtcgctg
    38881 cagtcattgc ttcgcaagca ttgattacag gttcgtttac gctagtttca gaagcaatca
    38941 agttgaaact gctgcctcgg ttaaagatca tgtatcctgg gaacagtatc gggcaaatgt
    39001 atatcccagc agtcaatctg attttatgga tcgcctgttc tctagtggtt gtcacattcc
    39061 gaacgtctca tcatatggaa gctgcttacg gtctatcgat cactgtgacg atgttgatga
    39121 caacagcact gctttacttt tatcttatcc aaaatgggca ttctcgttgg cttgcatatt
    39181 tagttacctt tttcttcggt gcgatagaag tcgttttctt tatctcaagt gttgtcaaat
    39241 tcttccgagg cggttttgta gccgttctga ttggtttgat catcttagtc gtgatgttta
    39301 tttgggaaca agggaacatt attcgcgaat cgtcaaccga ggatgtgtct ttgagagatt
    39361 atatcccgca gcttagagaa ttacgtgatg atacttcttt accaatctat cagacaaatg
    39421 tggtcttctt ggttcctgat atggctgaag ataaagttgg ccgacagttc atctattcga
    39481 ttttagataa acggcctaaa agagcacgag tctattggtt tgttcatgtg gaagtgacgg
    39541 atgagccata tacgaaagaa tatcaagtag atatgatgga cactgatttt attgtccaag
    39601 tgaatctgtt cttaggtttc agggtccaac aggaaatcaa cgtatatgtt cgccaagtca
    39661 tccatgattt gatgaaacaa ggccgtttgc caaaacagcc acaaagatac tctctgacac
    39721 cagggcgaga agttggcgat ttccaatttg tcctgattga tgaagtagtt tccaatgtga
    39781 caactctagg aaaatggcaa cgtcaaatca tgcaggcaaa actggcaatc aaaaaaatcg
    39841 ctacctcgcc agaaacatgg tttgggctag agtacagcga agtaaaacat gaaactgttc
    39901 cgcttatcat tggaaatact cggaaaacat ggttgaaaga gcggttgtga aagtagcgct
    39961 ttgacctgaa ctaatgtcga atgtcagcta cttgaacacc gtttatcaga ggtcatgaaa
    40021 aagctgtact gcaacaagca gtaagaacga gcagataaaa acagcttctc atgtttttca
    40081 tctgtttggg cttaatgccg tagatgcaag cttttgaata ccgtttttat cagaggtcat
    40141 gaaaaagctg tttagcaaca agtaacgcaa tgattttgaa gtgataagga gatgtggctg
    40201 aaaaatgccg catctccttt tttattccta aatatgaata cgcattattt tcaatctttt
    40261 tgtagaaaaa gggtacaata gaaaaagaga aaggcgggag aatatggtta tcaatttcat
    40321 tattttattt ttgatgattg caattacttc attttttgta gcaagtgaat ttgctttagt
    40381 gaaaatcaga cgatcaagat tggaacaact agaaaaggaa aatgtaaaga acgccaagct
    40441 agctcttcat gttacacacc atttagataa ctatttatca gctagtcaat taggaattac
    40501 gctaaccggt ttgatcatcg ggtgggtcgg tgaaggatct gtcgcggctt tactagaacc
    40561 agtgatcggt aaactacctt ttagttcggc tattagcagt acaatctctg tggcgctggg
    40621 atttatttta gttacgtata tcgatgtggt tattggcgaa ctgcttccta agagctatag
    40681 tatcgtcaat acggagaagg tcgtcttgtt cgtagttaaa ccacttcatt atttttataa
    40741 agtaatgttt ccttttattt gggtattgaa tcattcagct gcagggctag ggaagctatt
    40801 aggtgtgcgt ctcgtatctg aaggagaaga gacattgtct caagaagagc tgacattagt
    40861 cgcattgaat tcttatgaaa aaggagagtt gaccaaagaa gaatattatt atttggaaaa
    40921 cgtgtttgaa tttgatgata ccttggcaaa agatatacaa gtagaccgta cgtccatgca
    40981 agtattcgaa gcttcagaaa ccgtcaaaga agcgataaaa gcttcgattg aacaaggaca
    41041 tactcgctat ccggttatct tagaatcaaa agacaatgtt ttaggttatg tcaccttgcc
    41101 agatttgatc aagcaatctt ataaagatga ccagctgaca gtagaacagt tgattgaaga
    41161 gccgatcgta acaactgaaa cgatccctat aaaaaaatta ttgacgatca tgcgaaaaaa
    41221 gggtaagcat attgctatct taaaagatga atacggaggg acaagcggct tagtgacgat
    41281 tgaagatatt ttagaagaaa tcgtcgggga aatccgagat gagacagatt tggacgaagc
    41341 attgatagcg gaacagtcag atggctctta tatcatctcc ggcaaactaa cattagatga
    41401 tttccagcgt tattttcatg tagagattcc agagtttgaa gaaacgaatt ttactacact
    41461 tgcgggcttt gcttctagcc gatataaaga aataaaagca ggaacaatca ttgagattgc
    41521 atcattccgt ttcacggtat tagaatacca gcatgcacat atcgattatt tcaaagtaga
    41581 gtccacggaa agaaaaacag aataaaagaa aaaaggctgt gacatttttt gtcacagcct
    41641 ttttgttatc taaaaaacct gttttcggta aacgaagaag atattttcaa taaaagtttt
    41701 catttttagc cgcaacgatt tcttttaatc aaggctatct tgcttctgca tgttctaatg
    41761 tgaaatgttc ctcttcaccc atgtgtttca attgtttcat cgaacgcata taagagtaaa
    41821 gcaacacggc aacagaaccg atccaagcta aaggagaagc tgctgctgct cctgggtatc
    41881 cccacattcc tgtaagaacg atagctgcaa aagaacgcat aagcaattcc atcataccgg
    41941 cgatcgtagg gatcttgctt tgtccaagcc cttgaagtgt gtagcgtaaa atgaacagga
    42001 ctgctaagat ccagtaaaga gacccattga tattgaagta ggtttgtgct aattcaaata
    42061 cacgtgtttc tgatgggcta acaaacaatt gaacaaagaa atgaccgaag aaaatgacga
    42121 tagcacctgc taagagacta aatccgacac tcatcagtaa agtttgacgt accccttgca
    42181 agattcggcc atattctttc gcaccatagt tttgtgcagt gaaagttgcc attgcaatac
    42241 caaatgacat catcggctga tttgcaaatt ggtcgatacg accagcagcg gcttgagcag
    42301 ctacgacatc tgtccctaga ctatttagtg ctgcttgtaa cacgatagca ccaacagcga
    42361 tgatcgaaga ttgaaacgcc attggcaaag cagcatttag gtgtacagca aactcttctt
    42421 tttcaaattt gaaatctttt ttgcgtaact gtaacaaagg catttttttc ttgatataca
    42481 ctagacacag tacgctggaa gaaacttgtg cgattactgt agcaatagct gcaccttcca
    42541 cacccatgcc aaaatagata ataaagatca aatcaagtac gacgttgatg atgaccgcaa
    42601 tgatcagaaa gaatagtggt gtgcggctgt caccaagtgc gcgaatgaca ttagaaagca
    42661 aattgaaact gactgatgca aagatcccta ataaaataat tgaaataaat gtttgtgctt
    42721 gatcaagtat ttcaggagga gtttgcatca attgaagcat cggacgaata aacaggagac
    42781 ttaggacagt taaaatcaca gtgacgacaa gactaatgac aacactagca gcaaaacttt
    42841 tcttcagacc ccgataatct tttgcaccgt aacgttgtgc ggtaataatg gctaaaccag
    42901 ctgttaaacc ttgggcaaag ccaatgatca aaaatgtcag gcttccagtt gcaccaaccg
    42961 ctgccagcgc atttttacct agcgtctgac ccacgatgat catatcgatc atattgtaga
    43021 actgctgaaa aacatttccg atcagtaaag gaacagtgaa catcaaaatc agtttagcgg
    43081 ggcttccttt tgttaaatcg cgcatctttt aaactcctta tatctttaaa atttagttag
    43141 tagtatatag cgtttatcct gagaatacca attagaaatc aatgataatt tatgaaactc
    43201 cataaaaaga agccaagaat tgagtcaaag aatcaacaac ttggcctggt ctgtaaaata
    43261 agggacatcc acgtttatct atcaccttaa tattgtctaa tctcacttgg taaaaggatc
    43321 gtttctctcg taccgtcatc ataggcgtat acttgagcag gaaattggtt cgaataggca
    43381 aacggcaaat caatatccat ttccacattg cttcctgctt gagaaaaatg catcgtcact
    43441 tttccacgat tttcgatcaa atcaaaggtt aatatatttt ctaatggaat gactccctta
    43501 agatctaggt caataataaa ccatatactg tcaatcaatt catctggcaa acttgatacg
    43561 acaccaactg aagcgtaacg gttgcggctg ctatcaaaac tttcaaacat ctgtttaacc
    43621 tccttcgaat caacgaatgt tttattttta taattaatcc taggataaac tagaaatact
    43681 tacttcaacg agggttcatt ctaataaaga atcaggaaaa atgcaacaat aattgaatac
    43741 tttttttcaa agt
//
LOCUS       DACDYJ010000019        42857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDYJ010000019 DACDYJ010000000
VERSION     DACDYJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.019"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(522..878)
                     /locus_tag="IWM51_001012"
     CDS             complement(522..878)
                     /locus_tag="IWM51_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP9970447.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(892..1302)
                     /locus_tag="IWM51_001013"
     CDS             complement(892..1302)
                     /locus_tag="IWM51_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9970448.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1649..3835)
                     /locus_tag="IWM51_001014"
     CDS             complement(1649..3835)
                     /locus_tag="IWM51_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9970449.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4053..4736
                     /locus_tag="IWM51_001015"
     CDS             4053..4736
                     /locus_tag="IWM51_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP9970450.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4817..5536)
                     /locus_tag="IWM51_001016"
     CDS             complement(4817..5536)
                     /locus_tag="IWM51_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9970451.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(5848..6054)
                     /locus_tag="IWM51_001017"
     CDS             complement(5848..6054)
                     /locus_tag="IWM51_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970452.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6299..6901
                     /locus_tag="IWM51_001018"
     CDS             6299..6901
                     /locus_tag="IWM51_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP9970453.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7042..8322
                     /locus_tag="IWM51_001019"
     CDS             7042..8322
                     /locus_tag="IWM51_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9970454.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8335..9063
                     /locus_tag="IWM51_001020"
     CDS             8335..9063
                     /locus_tag="IWM51_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9970455.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(9132..10655)
                     /locus_tag="IWM51_001021"
     CDS             complement(9132..10655)
                     /locus_tag="IWM51_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9970456.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            10907..12088
                     /locus_tag="IWM51_001022"
     CDS             10907..12088
                     /locus_tag="IWM51_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9970457.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(12148..13776)
                     /gene="treC"
                     /locus_tag="IWM51_001023"
     CDS             complement(12148..13776)
                     /gene="treC"
                     /locus_tag="IWM51_001023"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP9970458.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(14173..16884)
                     /locus_tag="IWM51_001024"
     CDS             complement(14173..16884)
                     /locus_tag="IWM51_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9970459.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(16894..17595)
                     /locus_tag="IWM51_001025"
     CDS             complement(16894..17595)
                     /locus_tag="IWM51_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9970460.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            17781..18380
                     /locus_tag="IWM51_001026"
     CDS             17781..18380
                     /locus_tag="IWM51_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9970461.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            18461..19474
                     /locus_tag="IWM51_001027"
     CDS             18461..19474
                     /locus_tag="IWM51_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9970462.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            19582..20499
                     /gene="rbsK"
                     /locus_tag="IWM51_001028"
     CDS             19582..20499
                     /gene="rbsK"
                     /locus_tag="IWM51_001028"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP9970463.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            20527..20922
                     /gene="rbsD"
                     /locus_tag="IWM51_001029"
     CDS             20527..20922
                     /gene="rbsD"
                     /locus_tag="IWM51_001029"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP9970464.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            20922..22295
                     /locus_tag="IWM51_001030"
     CDS             20922..22295
                     /locus_tag="IWM51_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9970465.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            22652..24796
                     /locus_tag="IWM51_001031"
     CDS             22652..24796
                     /locus_tag="IWM51_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9970466.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(24848..25888)
                     /locus_tag="IWM51_001032"
     CDS             complement(24848..25888)
                     /locus_tag="IWM51_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9970467.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(26165..27967)
                     /locus_tag="IWM51_001033"
     CDS             complement(26165..27967)
                     /locus_tag="IWM51_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9970468.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(27981..28733)
                     /locus_tag="IWM51_001034"
     CDS             complement(27981..28733)
                     /locus_tag="IWM51_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9970469.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(28742..29104)
                     /locus_tag="IWM51_001035"
     CDS             complement(28742..29104)
                     /locus_tag="IWM51_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9970470.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(29280..30152)
                     /locus_tag="IWM51_001036"
     CDS             complement(29280..30152)
                     /locus_tag="IWM51_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP9970471.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(30178..30690)
                     /locus_tag="IWM51_001037"
     CDS             complement(30178..30690)
                     /locus_tag="IWM51_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP9970472.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(30828..31658)
                     /locus_tag="IWM51_001038"
     CDS             complement(30828..31658)
                     /locus_tag="IWM51_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP9970473.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(31674..32282)
                     /locus_tag="IWM51_001039"
     CDS             complement(31674..32282)
                     /locus_tag="IWM51_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP9970474.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(32275..32592)
                     /locus_tag="IWM51_001040"
     CDS             complement(32275..32592)
                     /locus_tag="IWM51_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9970475.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(32748..33476)
                     /locus_tag="IWM51_001041"
     CDS             complement(32748..33476)
                     /locus_tag="IWM51_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP9970476.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            33635..34516
                     /locus_tag="IWM51_001042"
     CDS             33635..34516
                     /locus_tag="IWM51_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9970477.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(34753..35796)
                     /locus_tag="IWM51_001043"
     CDS             complement(34753..35796)
                     /locus_tag="IWM51_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9970478.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(35799..36467)
                     /locus_tag="IWM51_001044"
     CDS             complement(35799..36467)
                     /locus_tag="IWM51_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9970479.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            36609..38945
                     /locus_tag="IWM51_001045"
     CDS             36609..38945
                     /locus_tag="IWM51_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9970480.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(39060..39371)
                     /locus_tag="IWM51_001046"
     CDS             complement(39060..39371)
                     /locus_tag="IWM51_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970481.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            39552..41027
                     /locus_tag="IWM51_001047"
     CDS             39552..41027
                     /locus_tag="IWM51_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP9970482.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            41074..41658
                     /locus_tag="IWM51_001048"
     CDS             41074..41658
                     /locus_tag="IWM51_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP9970483.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            41660..42079
                     /locus_tag="IWM51_001049"
     CDS             41660..42079
                     /locus_tag="IWM51_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP9970484.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(42360..42644)
                     /locus_tag="IWM51_001050"
     CDS             complement(42360..42644)
                     /locus_tag="IWM51_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP9970485.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
BASE COUNT        12773 a         8565 c         7548 g        13971 t
ORIGIN      
        1 gagtgtttcc cgattcgttt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacaact cagttatctt atcatgtcat
      241 ttctaacatg tcaacaacta ttttatatgt tttttaaaat actattttat tgctttgcta
      301 cttaaaacaa cagaaatgaa tatatcatgt aaatttacac atgtcaatat atttttaact
      361 ctatatcccc acctagattt gaattttgac cacataaatt gagcatgtct ctatagtaga
      421 aaaataaagt caaaaaaaga acacataaca cgcttgatgc taagtgttcg actgttacaa
      481 ctgtatgaaa tgctcagtga cttgattaaa acagttaaag tctatttatt taagataagc
      541 tgttccatca actgctcatt gtatccaata aatagaactt catcgataat aatcaaagga
      601 cgtctgagga gattggtatg ttgtgtgcat aaaaaaatca gttgtttttt tcctacacag
      661 gaaaggtcaa tcgcaatgtt gtgaaaagcc tcagaccaag tcgcaaatag ctgttctgct
      721 tgcacttctt caaaactgca taattcttct gcttcttctg tacttagatc gcgttcttct
      781 aagtcaacaa attgataagg caattgatgg tgttctaacc attgtttcgc cttcagactt
      841 gttacacagt gcgctgatcc atagactttc acttccacga aggctccctc cctatgaatt
      901 taagatgtta ttgttttgta aatagatcag atttctggca acacatcgaa tttcacgagg
      961 gacaaatgac cggatctcat cttcattaaa tccgattagt attctttttt catcaatcaa
     1021 aataggtcgt ctcaataatg aaggatattc tttgaataag acgatcattt gctttaaagt
     1081 taattcgtta aaatctatgc ccagcttttc gtaaattttc gaccgagtgg aaataatatc
     1141 ttgaactcct ccttcagtcg ctgccaagat ctttttgatc tcactttctg ttaacggttc
     1201 ggcaaaaata ttcttctctt caaattcaag ttgattcgct tgcaaccatg ctcttgcttt
     1261 tctacaggaa gtacagctag gcgaagtata tacttttatc attttctcac tcatttttaa
     1321 ttataatcca attctacagt aatttctttt aaaataccaa ttattagcta ataatatata
     1381 aaaaaaaatc aaaataactg attatttacg tttgaataat gagaattttt ctatatattc
     1441 tctattttga taatagtatt ctcattttca aaataaataa gggaggtgtg acaaaattct
     1501 tgtcacacct cccttttccg aataaactgt gttccagaaa tagcttcttc gaaaataagc
     1561 tgaaatttca caaaaatttg agaagcaatt ttcgtacatt ccttcttctt tcttgaaact
     1621 gaccacttct gtcacaccct ctcttctatc actttccttt tttcacacga ctcccggttt
     1681 ctttgataaa tctcgatact ttcaattttc ttgaattttt catttgaggt acacatagat
     1741 acagacgatt tcttgctcta gtcatcgcaa cataaaatag tcttcgttct tcttctatcg
     1801 tcgtcactgg ttctgctttt atttcattaa agtcgattac tttcttttct gtcgctccgt
     1861 tataaggcaa acttccttcg ttacaaccaa tcaaaaagac atttcggtaa ctaagacctt
     1921 tacttttgtg aatgctctgg atacatagtg catcaagttt tccttgtctg taagcaatca
     1981 agcgggtttt taattcttta gctgtcgctt gggtttcttg cgataaggct tggagtcttc
     2041 tgaaagtagc atgctgcttt acttcttctc gtatccattc ttgatcactc tggtcgattt
     2101 ccttagctat acgtggcaga tgaagatagc tttgaacaag cgaatagatt tcttgatgct
     2161 ggtctgtttt acgcaacgct tcctcaaact ctcggatttc tgcagatttc gattttaaat
     2221 tggggcttat cgacaaaata taatctgcaa cggctatatt ttgatattta ggagaaaagt
     2281 attgatcgca ttcacttttt ttcaggtagc agtctgccat cagctgccgg agcaattgct
     2341 ggtaggcctg atttttcttt tgcagattca gtgctgtttg ccaatgaaca attttttgat
     2401 agaaatgttg gaaagacttg ataccaaaga taccttcata tggactatct gcttccagta
     2461 caaaaggaat attcgttttc agcagagatt ggacaagctg ctgcgtttgt gaagctgatc
     2521 ggtataatat gattgattcc ttatatttcc catttttttg aataaatgtc tgaatttgtt
     2581 tgattagcca ctgactttct gctttagtat cagaaaacaa ataggcttcc ataacacctg
     2641 cttctttttg tgctggaatc atcgcctttg gtatccgatc atttttatta tagctgatca
     2701 atctatttgc tccttggaca atagctgccc gagaacgata attagtcaat aagaaaagag
     2761 ttttggtagg atattctttt tcaaagttct tgatgtatgc tggctctgat cctctgaatc
     2821 catagatact ctgatcatca tctccaacaa caatcaactt gatcctgttt tcctttgtca
     2881 tccgtttcac gatttcccat tgaagtggat tagtatcttg aaattcatca atgaatagat
     2941 aatcatattt ttggccgata taatgctgga tctcctcatt ctccaaagcg cttttcagat
     3001 tttggagtac atcattgaac tctagcaatt gctggcttct tttttgttct tggagttccg
     3061 tgagcaatcc atacagtcgt tctgatatcg gctgtcgtgc attcttatca atggaatcat
     3121 atttttcttt aactaactga accatttctt ctactgaaag gttcatattc tgccaatagt
     3181 ctaaccgaga agcaatcgtt ttttctccat attgatcaaa tatataaaga ttatatttac
     3241 gtatcagctg ttgcaaaaca gcattcactt tttgtgcagt atcctttgaa aagtctcctt
     3301 gtattccttg cattgctact tcaggaaatt ttcgacaaat ttcattgtat atcctaaaaa
     3361 agatactatg gaatgttccg atatggacag cggtttttct ctggctgaaa ttatctactc
     3421 gtttttttag ttcatttgca gcgttccttg taaaagtgat cattagtatg cgttcttcgt
     3481 ctacttgttt ttgcgtcatc agataaacag cttttgcaca gattgtttgc gttttgccgc
     3541 tcccagctcc tgctatgatt cgaagatgtt tcttcatatc atatgtgata gctgcttttt
     3601 gttcttgatt gaagcgatta taggaacgtt tctccatcat tttaaagaaa gagctgtttc
     3661 cttcactttc ttttacttgt acttcttcct caataagtcc gggtgcttcg ccataagccg
     3721 cttcataggt agcaatgatt tcttcttgta gccgaaagac ttgttgaagc tttttatact
     3781 tgttctccca gcgtttcaat tctttttgta atcggagaat ttctttttca ttcaactgat
     3841 tgatttcctt cccatctttc ttctttgaat ttgtttattt caagttggtt atttaaagtt
     3901 tcgttgtcta ttttttcttt atactaacag acctttctaa aaatgaaaat gggaagcgat
     3961 atgattttag ttttctgaaa atttttcctt tttttcaatc gtttatgcta agataaggaa
     4021 gacaaaaata ttcataaaag gtgatgaaaa aaatgcagac ttcctttcac gaggggcgta
     4081 caataggact tttgctgaca tttatcggag gagccatgga cagttatact tatatccaat
     4141 atggtgcttt tgcctctgct caaactggca atatcattct atcgatcatc caagcatttg
     4201 acggagaatg gctaagtgtt ggtaaaaaag tattgtctac ccttttcttt ttcttaggga
     4261 ttcttctaac aaagtatcta attgattatt ttagaaaaaa agaaaaacat ttctggcgct
     4321 tgtttgttct atattatgaa gcagtgattt ttttctttgt tagtttgaca cccgtccatg
     4381 agcatcccgc attagtaacg attttgattg cttttactgc atcgatccag tgggtagctt
     4441 ttgataaaat caacgggcgt gcctacacta atttatttac aactggaaat ttaaaagggg
     4501 tagcaacgaa cttatatgat tattgtattt ctaaagaaaa agcagatttt gatcgatttt
     4561 tccactatct aagtgtcgtt cttgccttta tcgcaggagc tattgtcctt gttttctgtc
     4621 atcatctgtt tggcgcccat tcgattttga tcgtttccat tttatttttg atcatggccc
     4681 tcactcagac atttcattta tggcaatttt atcatacgaa tcggttcact atttgatctc
     4741 aatgaatcaa aaaaactaag caagatgctg ctcacagaga aatatctgcg agtgagcaat
     4801 ttgcttagtt tttatattat ttatttgtaa agattccctt tgctttcggg ttatttgtag
     4861 tgtagcggat cgactgggca tctgcctctt ctgtcatttt ctttgcccgc atcttgattt
     4921 cttgtacgat ttgttctcca catgccggat cggctacgaa atcatatttg tccccatcga
     4981 tcactatttt gggacatata tcaaactgtt cataccactc ttcgtatcgt cgattgagtt
     5041 ctttatagta ggaatagagt tcaggatctt gttctagctg ttcatattcc cggcctcttt
     5101 ttttgattct cgctaacata gtttcaaagg atacacgaat ataaaccagt agatctggtt
     5161 tcttttgtgg cgaaatttct tctaactcct tcagcatcgt ttctaaaaga ccttcatact
     5221 gctttacttc tacttcagaa acacgcccta aatctgcatt caaatgaaac agcatactat
     5281 cttcgtagat ggagcggtct agtacgttgt catcatggct gaaagcatct ttgattgcca
     5341 aaaagcgttt gttcaaaaag aaaatctgta ataaaaaagt atacttttct ggattagaat
     5401 aaaatagcgg caatacttca ttatcttcca cgttctcata aaaaggtttt gtttctaaat
     5461 cttgggcaag catttcagtt agtgtgcttt tccctgcacc gatcgttcct gcaacaatga
     5521 tcacaattat cggcatcctt cctgtaataa aatctgttct tcattttact acttcattta
     5581 tagaaataaa agccttgact ttatctgctc cttaaaagct acttctctaa aagtagccaa
     5641 aagtaactcc tattcatgaa aataaaggct gtgacaaaag tcttgtcgca gccctttttt
     5701 caaataaaca gtattctcct gccaacacct cggtcacaag gcaatattca taaaacatga
     5761 aaagctgttt tttaacatta ttccttgtaa ctcagagcta aacgggagac tcacaacctc
     5821 tttatgctct caacatttta tctgttttta ttcttctatt gtacttgatt tgcgtacaac
     5881 taactgccag tgcagattat gagcggtatc attgtcttct actgcgcatt tcatgacacg
     5941 tatcatcaca ctattttcat agattttttc tatttttcct tttattggtg attcaaataa
     6001 atcagattta aatgcatatg tttcgccgac ttcaaacaat tgttcatttt ccataagaat
     6061 ccctccatca ccttttataa ggtcagtaat tttttaacat aaatcactcg aaaaagcaca
     6121 aacttgtcat aaaaagtact tttcgtatca aaactgtcac tttttttggt aaggttcttg
     6181 gaaaacgaag atttttttag gagatcatga cggttttgat gtcaaaacca ctttttatga
     6241 aacaaaagac gttttttatt ttttttagca taaaatttgt ggtggaggta cgtatgagat
     6301 gaaagataaa gttgtgaaca aattattaga agccttattc agcggagaca aatcggaaga
     6361 agatatggtg tatgtccaag taaaatttgc cttagaagtt ttattgaata atcttggaaa
     6421 actagcggtc gttctagtct tttctctggt gacaggctca tgggcggaaa caggaatcac
     6481 ctttctttct tatatctgca tccgcagata tgcttatgga ttgcattccg actctgaatt
     6541 cgtttgtctg ctgtggacac tactttattt atggggcgtt cccttggtga tgaaacatct
     6601 gcaattgact atctccttcc caatgatggt ctttttgtta atcagctgct ttttgttact
     6661 gcttcgctat ggatcaagag gtacagcaat caatcccatt gaacccgaga aacgtccccc
     6721 attattaaag aaggcgatca gcatgtttct aattttttct ctcattactt tatttttctc
     6781 agcttcttac ttttctacct atttgctact tgggattgtt ttagaaatag ctacgttgct
     6841 tcctattaca aactacttga tgaattttgg aggaatcttt catgaaaaaa acaatcatta
     6901 aacaattaac taacttatct gacttttttg tatctggctg ctgctttggt atgagttctg
     6961 aattcaaaat ccctgaaaaa ttaaaaaaat aatacgctaa ttgtgctata atttggtaaa
     7021 aatcaatggg agctgtactt tatgttttta tggtccatag cgttgcaata tttgatttac
     7081 acaatttgta tcttattgat tgaccatcac atccgataca aatatctgtt tgcgggagtc
     7141 attctgattt cttgtatttt gattggatgg gtctattcga ttttagggat ttttactgct
     7201 ctaattgcta taggaatgct gatgcttact tttttcttct tttcgacttc gcgtcaacaa
     7261 ttggtttacg cgcttccttt tgggttattg acgtcgcttt taggagatca cttaacaagc
     7321 atcgacgatt tttttgtttt gaaatcttcg gttattgaac caggagattc attgcagtat
     7381 tttcacattg tcttatccgc ttttttatct gctttattag ctttttgttt aaaaaaatcg
     7441 ttagagactt ggtttcacac tgtggaccgc cgtatgatcg gtacgatcgg gacattgata
     7501 ttatttacat actatatcat tattttttat acccgttttt ctggtgaaac acctgatgtc
     7561 ttagctttga atacgagttt tttcattcta tatctgtgtg ttggcctgat catcttggtt
     7621 tattattgga aaatctctaa taagaaatta gctgatcagt tattagagca acaagtcagg
     7681 atgcaacagg attatatcaa agatctagaa aaaaactatc aagacttgag agaattcaaa
     7741 catgattacc aaaatctgct tttttctctt cacagctata tttcagaagg cgacttagaa
     7801 ggattacggt cttattatga tcaaacgata ttacctacac gccagatgat gaaccttttt
     7861 ccggccaatc taagtatgtt ggataatatg aaagttcctg agatccgtag tttactgtca
     7921 ttaaaactca tgatggcaca ggaaaaaggg ttgatcgtcc agcttcattt tcctgagcca
     7981 atcgaactgg atacgaaaca tacggtaaac cttgtacgta tgcttggtat tatcttggac
     8041 aatgcgattg aaggagctag tgctgcaaag aagaaaaaaa tcgaattgac catactcaag
     8101 aagaatcatt ccgtgatgat tcgtgtgatc aatacaacaa caaatacatt accgttaaac
     8161 cagttgaaac aaaaagggtt ctcaacaaaa acaaatccaa aaaacgaagg ccttggcttg
     8221 tttatcttgg atgaattgac aaagaccaat ccttatcttt tccttgaaac atcaattgaa
     8281 aaccagcgtt tttctcaaac gatttatata caaataaaat aaaggagtaa tgatatgatt
     8341 cccatttata tttgtgaaga cgatccaaaa caattagaag ttttatcaac ggtgatcaaa
     8401 aatagaatca tgattgaaaa cttagacagt tatgtccata tttctacctc tgatccggaa
     8461 gaacttctca atgcagccaa gatcagaact tcttcttttg gtatttactt tttagatatc
     8521 gaactagaag acagtgaaat ggatggtgta gcgatcggta aattgatccg tgaattagat
     8581 cctttaggaa aaataatcta tgtgacttcc catacggaaa tgtcgatgaa aatcttaaaa
     8641 agcaacatcg aaccgactga ttatatcgtc aaagaagatc tttatgattt aaaagaaaat
     8701 gtcgagcata tcctgagcaa aattttcgaa gattttcaaa tgtcccaaat ccaaaaagat
     8761 atcttcaaaa tagagtttaa tgatgagatc aaatttttac cgatcaaaga catccaatat
     8821 ttttctactt cgctaggagc gccacataaa ctggaagtcc atttgactca tgcccaatta
     8881 caattctatg agaagatcaa agaaatcgag aaaatgcatc ggaattttat ccgttgtcat
     8941 aaatcctatg ttatcaatac acaaaatgtg cggtcgatta ataaaaaaac cagagaagtc
     9001 acgttggcaa acggcgagac catcccagcc tctatccgtg gattaaagaa attgatttca
     9061 taaaaagaac cgaatagttt ggaccgacta aatgaccagc cgatccaaac ccttcggttc
     9121 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
     9181 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
     9241 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
     9301 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
     9361 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
     9421 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
     9481 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
     9541 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
     9601 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
     9661 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
     9721 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
     9781 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
     9841 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
     9901 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
     9961 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
    10021 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
    10081 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
    10141 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
    10201 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
    10261 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
    10321 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
    10381 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
    10441 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
    10501 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
    10561 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
    10621 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
    10681 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
    10741 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
    10801 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
    10861 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
    10921 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
    10981 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
    11041 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
    11101 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
    11161 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
    11221 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
    11281 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
    11341 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
    11401 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
    11461 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
    11521 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
    11581 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
    11641 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
    11701 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
    11761 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
    11821 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
    11881 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
    11941 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
    12001 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
    12061 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
    12121 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
    12181 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
    12241 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
    12301 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
    12361 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
    12421 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
    12481 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
    12541 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
    12601 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
    12661 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
    12721 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
    12781 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
    12841 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
    12901 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
    12961 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
    13021 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
    13081 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
    13141 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
    13201 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
    13261 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
    13321 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
    13381 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
    13441 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
    13501 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
    13561 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
    13621 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
    13681 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
    13741 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
    13801 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
    13861 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
    13921 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
    13981 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
    14041 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
    14101 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
    14161 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
    14221 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
    14281 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
    14341 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
    14401 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
    14461 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
    14521 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
    14581 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
    14641 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
    14701 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
    14761 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
    14821 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
    14881 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
    14941 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
    15001 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
    15061 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
    15121 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
    15181 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
    15241 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
    15301 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
    15361 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
    15421 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
    15481 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
    15541 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
    15601 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
    15661 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
    15721 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
    15781 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
    15841 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
    15901 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
    15961 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
    16021 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
    16081 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
    16141 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
    16201 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
    16261 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
    16321 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
    16381 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
    16441 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
    16501 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
    16561 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
    16621 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
    16681 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
    16741 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
    16801 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
    16861 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
    16921 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
    16981 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
    17041 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
    17101 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
    17161 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
    17221 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
    17281 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
    17341 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
    17401 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
    17461 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
    17521 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
    17581 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
    17641 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
    17701 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
    17761 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
    17821 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
    17881 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
    17941 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
    18001 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
    18061 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
    18121 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
    18181 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
    18241 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
    18301 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
    18361 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
    18421 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
    18481 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
    18541 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
    18601 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
    18661 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
    18721 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
    18781 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
    18841 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
    18901 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
    18961 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
    19021 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
    19081 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    19141 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    19201 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    19261 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    19321 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    19381 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    19441 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    19501 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    19561 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    19621 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    19681 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    19741 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    19801 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    19861 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    19921 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    19981 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    20041 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    20101 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    20161 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    20221 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    20281 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    20341 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    20401 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    20461 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    20521 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    20581 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    20641 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    20701 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    20761 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    20821 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    20881 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    20941 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    21001 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    21061 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    21121 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    21181 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    21241 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    21301 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    21361 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    21421 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    21481 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    21541 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    21601 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    21661 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    21721 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    21781 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    21841 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    21901 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    21961 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    22021 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    22081 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    22141 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    22201 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    22261 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    22321 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    22381 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    22441 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    22501 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    22561 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    22621 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    22681 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    22741 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    22801 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    22861 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    22921 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    22981 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    23041 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    23101 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    23161 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    23221 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    23281 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    23341 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    23401 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    23461 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    23521 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    23581 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    23641 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    23701 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    23761 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    23821 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    23881 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    23941 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    24001 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    24061 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    24121 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    24181 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    24241 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    24301 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    24361 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    24421 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    24481 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    24541 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    24601 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    24661 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    24721 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    24781 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    24841 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    24901 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    24961 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    25021 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    25081 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    25141 atggatgatc gatgccaccc actatcattg attggcgaaa atcactggca aagacctcac
    25201 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    25261 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    25321 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    25381 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    25441 gcgtataccg tacttctaca tctaaagttc caggaaatcc gttttccata tctgggctag
    25501 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    25561 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    25621 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    25681 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    25741 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    25801 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    25861 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    25921 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    25981 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    26041 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    26101 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    26161 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    26221 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    26281 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    26341 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    26401 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    26461 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    26521 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    26581 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    26641 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    26701 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    26761 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    26821 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    26881 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    26941 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    27001 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    27061 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    27121 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    27181 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    27241 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    27301 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    27361 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    27421 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    27481 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    27541 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    27601 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    27661 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    27721 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    27781 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    27841 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    27901 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    27961 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    28021 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    28081 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    28141 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    28201 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    28261 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    28321 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    28381 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    28441 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    28501 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    28561 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    28621 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    28681 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    28741 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    28801 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    28861 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    28921 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    28981 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    29041 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    29101 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    29161 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    29221 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    29281 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    29341 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    29401 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    29461 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    29521 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    29581 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    29641 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    29701 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    29761 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    29821 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    29881 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    29941 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    30001 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    30061 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    30121 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    30181 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    30241 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    30301 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    30361 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    30421 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    30481 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    30541 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    30601 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    30661 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    30721 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    30781 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    30841 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    30901 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    30961 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    31021 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    31081 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    31141 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    31201 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    31261 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    31321 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    31381 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    31441 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    31501 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    31561 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    31621 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    31681 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    31741 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    31801 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    31861 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    31921 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    31981 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    32041 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    32101 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    32161 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    32221 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    32281 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    32341 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    32401 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    32461 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    32521 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    32581 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    32641 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    32701 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    32761 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    32821 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    32881 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    32941 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    33001 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    33061 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    33121 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    33181 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    33241 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    33301 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    33361 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    33421 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    33481 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    33541 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    33601 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    33661 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    33721 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    33781 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    33841 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    33901 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    33961 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    34021 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    34081 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    34141 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    34201 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    34261 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    34321 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    34381 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    34441 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    34501 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    34561 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    34621 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    34681 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    34741 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    34801 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    34861 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    34921 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagta caatctctgt
    34981 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    35041 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    35101 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    35161 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    35221 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    35281 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    35341 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    35401 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    35461 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    35521 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    35581 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    35641 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    35701 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    35761 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    35821 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    35881 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    35941 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    36001 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    36061 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    36121 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    36181 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    36241 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    36301 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    36361 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    36421 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    36481 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    36541 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    36601 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    36661 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    36721 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    36781 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    36841 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    36901 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    36961 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    37021 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    37081 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    37141 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    37201 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    37261 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    37321 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    37381 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    37441 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    37501 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    37561 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    37621 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    37681 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    37741 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    37801 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    37861 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    37921 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    37981 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    38041 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    38101 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    38161 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    38221 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    38281 tcgtcaaagc tcaaagggac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    38341 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    38401 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    38461 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    38521 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    38581 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    38641 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    38701 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    38761 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    38821 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    38881 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    38941 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    39001 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    39061 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    39121 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    39181 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    39241 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    39301 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    39361 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    39421 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    39481 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    39541 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    39601 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    39661 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    39721 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    39781 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    39841 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    39901 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    39961 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    40021 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    40081 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    40141 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    40201 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    40261 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    40321 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    40381 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    40441 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    40501 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    40561 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    40621 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    40681 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    40741 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    40801 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    40861 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    40921 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    40981 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    41041 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    41101 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    41161 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    41221 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    41281 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    41341 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    41401 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    41461 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    41521 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    41581 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    41641 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    41701 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    41761 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    41821 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    41881 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    41941 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    42001 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    42061 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    42121 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    42181 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    42241 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    42301 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    42361 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    42421 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    42481 ttcacgatac tgccttcata cggcacattg tcaaaagttg cttggacttt aactcttcca
    42541 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    42601 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    42661 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    42721 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    42781 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    42841 gctcacaacc tcttcct
//
LOCUS       DACDYJ010000020        39688 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDYJ010000020 DACDYJ010000000
VERSION     DACDYJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.020"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(78..635)
                     /gene="frr"
                     /locus_tag="IWM51_001051"
     CDS             complement(78..635)
                     /gene="frr"
                     /locus_tag="IWM51_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP9970486.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(638..1360)
                     /locus_tag="IWM51_001052"
     CDS             complement(638..1360)
                     /locus_tag="IWM51_001052"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP9970487.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1496..2377)
                     /locus_tag="IWM51_001053"
     CDS             complement(1496..2377)
                     /locus_tag="IWM51_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP9970488.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2476..3258)
                     /gene="rpsB"
                     /locus_tag="IWM51_001054"
     CDS             complement(2476..3258)
                     /gene="rpsB"
                     /locus_tag="IWM51_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP9970489.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3617..4096
                     /locus_tag="IWM51_001055"
     CDS             3617..4096
                     /locus_tag="IWM51_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP9970490.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4313..5404
                     /gene="phnW"
                     /locus_tag="IWM51_001056"
     CDS             4313..5404
                     /gene="phnW"
                     /locus_tag="IWM51_001056"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP9970491.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5397..6182
                     /locus_tag="IWM51_001057"
     CDS             5397..6182
                     /locus_tag="IWM51_001057"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9970492.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6528..8219)
                     /locus_tag="IWM51_001058"
     CDS             complement(6528..8219)
                     /locus_tag="IWM51_001058"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP9970493.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8641..9588)
                     /gene="arcC"
                     /locus_tag="IWM51_001059"
     CDS             complement(8641..9588)
                     /gene="arcC"
                     /locus_tag="IWM51_001059"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP9970494.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9703..10722)
                     /gene="argF"
                     /locus_tag="IWM51_001060"
     CDS             complement(9703..10722)
                     /gene="argF"
                     /locus_tag="IWM51_001060"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP9970495.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10813..12042)
                     /gene="arcA"
                     /locus_tag="IWM51_001061"
     CDS             complement(10813..12042)
                     /gene="arcA"
                     /locus_tag="IWM51_001061"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP9970496.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12503..13204
                     /locus_tag="IWM51_001062"
     CDS             12503..13204
                     /locus_tag="IWM51_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9970497.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(13776..14792)
                     /gene="tsaD"
                     /locus_tag="IWM51_001063"
     CDS             complement(13776..14792)
                     /gene="tsaD"
                     /locus_tag="IWM51_001063"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP9970498.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(14789..15253)
                     /gene="rimI"
                     /locus_tag="IWM51_001064"
     CDS             complement(14789..15253)
                     /gene="rimI"
                     /locus_tag="IWM51_001064"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9970499.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(15260..15802)
                     /gene="rimI"
                     /locus_tag="IWM51_001065"
     CDS             complement(15260..15802)
                     /gene="rimI"
                     /locus_tag="IWM51_001065"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9970500.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(15786..16610)
                     /gene="tsaB"
                     /locus_tag="IWM51_001066"
     CDS             complement(15786..16610)
                     /gene="tsaB"
                     /locus_tag="IWM51_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP9970501.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(16699..17679)
                     /locus_tag="IWM51_001067"
     CDS             complement(16699..17679)
                     /locus_tag="IWM51_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9970502.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(17703..19187)
                     /gene="galT"
                     /locus_tag="IWM51_001068"
     CDS             complement(17703..19187)
                     /gene="galT"
                     /locus_tag="IWM51_001068"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP9970503.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(19199..20188)
                     /gene="galE"
                     /locus_tag="IWM51_001069"
     CDS             complement(19199..20188)
                     /gene="galE"
                     /locus_tag="IWM51_001069"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP9970504.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            20437..20604
                     /locus_tag="IWM51_001070"
     CDS             20437..20604
                     /locus_tag="IWM51_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP9970505.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(20666..22477)
                     /locus_tag="IWM51_001071"
     CDS             complement(20666..22477)
                     /locus_tag="IWM51_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP9970506.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(22474..22839)
                     /locus_tag="IWM51_001072"
     CDS             complement(22474..22839)
                     /locus_tag="IWM51_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP9970507.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(23002..23397)
                     /locus_tag="IWM51_001073"
     CDS             complement(23002..23397)
                     /locus_tag="IWM51_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9970508.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(23415..24377)
                     /locus_tag="IWM51_001074"
     CDS             complement(23415..24377)
                     /locus_tag="IWM51_001074"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP9970509.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(24377..24589)
                     /locus_tag="IWM51_001075"
     CDS             complement(24377..24589)
                     /locus_tag="IWM51_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP9970510.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(24610..25308)
                     /locus_tag="IWM51_001076"
     CDS             complement(24610..25308)
                     /locus_tag="IWM51_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP9970511.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(25328..25870)
                     /locus_tag="IWM51_001077"
     CDS             complement(25328..25870)
                     /locus_tag="IWM51_001077"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP9970512.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(26002..27006)
                     /locus_tag="IWM51_001078"
     CDS             complement(26002..27006)
                     /locus_tag="IWM51_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9970513.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(27003..27992)
                     /locus_tag="IWM51_001079"
     CDS             complement(27003..27992)
                     /locus_tag="IWM51_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9970514.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(27989..28795)
                     /locus_tag="IWM51_001080"
     CDS             complement(27989..28795)
                     /locus_tag="IWM51_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9970515.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            28961..29917
                     /locus_tag="IWM51_001081"
     CDS             28961..29917
                     /locus_tag="IWM51_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9970516.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(29994..30512)
                     /locus_tag="IWM51_001082"
     CDS             complement(29994..30512)
                     /locus_tag="IWM51_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9970517.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(30600..30749)
                     /gene="rpmG"
                     /locus_tag="IWM51_001083"
     CDS             complement(30600..30749)
                     /gene="rpmG"
                     /locus_tag="IWM51_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9970518.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            30977..31423
                     /locus_tag="IWM51_001084"
     CDS             30977..31423
                     /locus_tag="IWM51_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9970519.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(31618..33513)
                     /gene="asnB"
                     /locus_tag="IWM51_001085"
     CDS             complement(31618..33513)
                     /gene="asnB"
                     /locus_tag="IWM51_001085"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP9970520.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(33838..34812)
                     /locus_tag="IWM51_001086"
     CDS             complement(33838..34812)
                     /locus_tag="IWM51_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP9970521.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(35148..35235)
                     /locus_tag="IWM51_001087"
     tRNA            complement(35148..35235)
                     /locus_tag="IWM51_001087"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35201..35203),aa:Ser,seq:cga)
     gene            complement(35380..35946)
                     /locus_tag="IWM51_001088"
     CDS             complement(35380..35946)
                     /locus_tag="IWM51_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9970522.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            36201..37532
                     /locus_tag="IWM51_001089"
     CDS             36201..37532
                     /locus_tag="IWM51_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP9970523.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            37498..37848
                     /locus_tag="IWM51_001090"
     CDS             37498..37848
                     /locus_tag="IWM51_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970524.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(37940..38359)
                     /locus_tag="IWM51_001091"
     CDS             complement(37940..38359)
                     /locus_tag="IWM51_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP9970525.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(38360..38704)
                     /locus_tag="IWM51_001092"
     CDS             complement(38360..38704)
                     /locus_tag="IWM51_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP9970526.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            39054..39650
                     /locus_tag="IWM51_001093"
     CDS             39054..39650
                     /locus_tag="IWM51_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9970527.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT        11518 a         8196 c         6898 g        13076 t
ORIGIN      
        1 tttaattttt aaataaaaga aatataaata aaaacagagg aagcggaatg atccgcccct
       61 ctgtttttat gattttatta aacttccaat aattcttttt ctttatcagc agcgatctta
      121 tctacttctt taatgctgtt atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa
      181 tcatcttcgg taatatcacc atttttttgt tgttttttat attcatcgat cgcatcacga
      241 cggatgttac gaatcgcgat ttttgcattt tctgcttctt ttttcacatc tttagctaat
      301 tcttttctgc gttcttctgt cagttgcggg ataactaagc gaatgacatt tccatcattg
      361 gttggactga ttccgatatc acttgccata atagcttttt caacatcttg aagaatgctc
      421 ttgtcaaaag gtgtgatcat taaaacacgt gcttctggaa tttggattga tgccatttgg
      481 ttcaatggtg ttggcgcacc gtagtagttc acagtaatac ggtccaaaag gctagcgttc
      541 gcacggcctg cgcggatttg cccaagttca cgttgaagat tttgtgctgc ttttttcatt
      601 ttttcttttg cttctgtcat aattgcatct gccattttta tttccccctt acagttgttc
      661 cgatattttc gcctaagatt gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa
      721 gcagtggaat atcattatcc atgcttagtg aacttgcagt tgaatccatg acttgtaatc
      781 ctttggcaat aacttccaga tgagtcagtt cttcaaattt tactgcattt gcatctactt
      841 tcggatcagc agaataaaca ccgtctacat tgtttttagc catcaggata acatctgcgc
      901 cgatttctgc cgctcgtaat gctgctgttg tgtctgtcga gaagtacggg ttgccggtac
      961 cgcctgcaaa gataacgatc cgtccttttt ctaaatgacg ttctgcttta cggcgaatat
     1021 atggctctgc gatttgacgc atttcaatcg aagtttgcac gcgtgtcggt acacccacgt
     1081 tttccaacgt atcctgtaaa gctaaggcat tcataactgt agctaacata cccatgtaat
     1141 cagcttgagc acgttccatg cccatttgtg ctccgatttg tccgcgccag atatttccgc
     1201 cacccacaac gattgccatt tcaacgccca gctcgtgaac ttctttgatt tcttggatga
     1261 tttctttgat tactggtggt ttgattccaa atcctgcatc cccagctaaa gcttctccac
     1321 tcaattttaa gactacacgc tgatatttcg gtgtgaccat gttcattcct ccaatatttt
     1381 ctattgccat tttaccatac ttagatgatt tcgacactct aagactggtt tttcttgttt
     1441 tgcttaaaaa aagggagcgc acaaaccgtg cgttcccgtc tgttattcaa cagaattatt
     1501 ttttcacttg gctcataact tcttctacaa agttatcttc gcgtttttcg ataccttcgc
     1561 caacttcgaa acgtacgaat gttttaacag ttgcgccttt tgatgcaacg tatttttcaa
     1621 cagtcatatc aggatcttta acgaatggtt ggtccaccaa agcgatttct gctttgaatt
     1681 tcttcaagcg tccttctacc attttttcaa cgatattcgc tggtttgcct tcgttcaatg
     1741 cttgttctgt aagaacagtt ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt
     1801 ttacataacg aggattgata gctgctacgt gcatagctac gtctcttgca actgtttcgt
     1861 cagttgttcc atctagaaca gtcaatacgg caatacgtcc gcccatatgc aagtatccac
     1921 caaatgcagc attgtcgtct ttttctacaa cttcaaagcg acggaagctg attttttctc
     1981 cgataacttg agttgcttcg atcaagtcag attcaattgt acctttatct gttttgattt
     2041 tcattgcagc ttccatgtct gctggtttgt tttcagcaac aagttctgca atttctttta
     2101 ctaaatcttg gaacatttcg tttttagaaa caaagtctgt ttctgagttt acttcaacga
     2161 ttgctgcagt gttacctttc acagcaactg aagccaatcc ttcagctgca atgcggtcat
     2221 ttttcttcgc agcctttgcc attccttttt cacgtaaaag atcaactgct ttttccatgt
     2281 cgccttctac ttcaactaat gcttttttcg catccatcat tcctacgcca gtcatttcgc
     2341 gtagttcttt taccatttta gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt
     2401 tctttttaaa aaaagctgct tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag
     2461 ctccatcatt caaaattatt ctgctgaaga attgtcgcct tcaacaacat ctacgatttc
     2521 ttcgattgat gttgctgtgt tttcttctgt gaaatcttct tcaactactt gatcttcacc
     2581 ttggtttcct tcgatgaaag catcagccat ttttgaagta atcaatttaa cggcacgaat
     2641 tgcatcgtcg tttgaaggga ttactacgtc gatttcatct ggatcgcagt ttgtatcaac
     2701 catagctacg attgggatat ttaatttgtg tgcttcttga acagcaatac gttctttacg
     2761 tgggtcaacg atgtacatta catctgggat tcttggcata tcagcgatac cgcctaagaa
     2821 tttttcaaga cgttcacgtt ctttgttcaa accagcaact tcttttttag gtagaacttc
     2881 aaaagttcca tcttcttcca ttgcattgat ttgtttcaaa cggctgatac gtttttggat
     2941 agtatcccag tttgtcaatg ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc
     3001 acgagttgct tcttctttga ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc
     3061 tacgccgcct tcttcagcaa cgctcttcat gtaatcataa gctgcatcta ctaatttaac
     3121 tgttttttgc aagtcaatga tgtagattcc gtttctttct gtgaagatat atttcttcat
     3181 ttttggattc cagcgacgag tttggtgacc aaagtgtacg ccggcttcta gtaattgttt
     3241 catagaaatt actgccatgt ttgtttcctc cgatttggtt ttatttttcc tcttcttatc
     3301 ttctaccttg ccggcaaact ttttcaagca cctaccgaca atccaataag aatgtggatt
     3361 tgctgatgca taaagcaccc tcagctatta tacagacaaa agatccgtat cgcaagagca
     3421 aatatacttt tctgctcaac attcaatagt ttacctgaaa gataaaaaga attcaagaaa
     3481 aacagtagat ttctctttgt ttctttatta tcctttttcc ataaaataat tttaggtata
     3541 aaagacgaat attattgggc aatccttctt ttttttgtta tgatacatgt gaaatctttc
     3601 gtaaaggaga aagaaaatgc gcaaagctca aagacatttg ctgattaaac aagtcattga
     3661 agaattcccg atccgtacgc aagaagaact attaaacaaa cttgggcatt atggcgttac
     3721 cgccacgcag gcaacgattt ctcgagatat ccgtgatcta aaaatcgtga aggcacctga
     3781 tgaaaatggc gtttcccgct tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac
     3841 tgaggaagag gatcggctgg ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt
     3901 ccactttttg acgatcgtca ataccttgcc agacaatgct catttatttg catctgtttt
     3961 agatgagatc aaacctccct ctattgtcag tacaattgct ggattcgata cgatcatggt
     4021 catctcggaa aatgaggagg acgcaaaacg agtagaagaa tttcttcacc atccaactga
     4081 agtatcactt ttttaatcag agcacgtatt cctaatatgg aatgcgtgtt tttttatttt
     4141 caatattttc cttattaaat aaacctttac actctttaca aaaatacatc attttttatt
     4201 caattttttc aacaccttga ttgaatcaca ataatcatca tttatattgg atgttaacaa
     4261 gagcagcttc ttgctctttg tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa
     4321 attattaact cctggaccat tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga
     4381 tcattgtact tgggatcaag aatacaaaga aattacacaa tggataagaa aaaaattatt
     4441 ggatcttgct caagtatcgg aaggaatata cacaacggtc ttaatgcaag gtagcggctc
     4501 ctttggggta gaatcggtat taagttctgt gatcaggcca gaagatactg tactgatttg
     4561 ttcaaacggc gcatatggac aacgaatgat caacatgtgc gaatgtctga aactgaacta
     4621 taccgtttat gaggtatcgg aacatcaaat cctccaagct catgtgatta cacagctgat
     4681 tgaagagaat ccctctatct ccgcactagc aatggtccat agcgagacta cttccggtat
     4741 tttgaataat ttggaagaga ttgctgctgt cacacaagaa aaagatcttt tgtttatagt
     4801 ggacgcgatg tcaagctttg gtgggatacc gattcgtgta gccgaactag ggattgattt
     4861 tttgatttcc agctcaaaca aatgtattca aggtgttcca ggcttttctt ttgtacttgc
     4921 taataaagcg gaactagaaa agcgaaaaaa tgtgagtcga agtttgtcac ttgatctata
     4981 tgaccagtac cgcgtgatgg aaaacgagaa tggcaaatgg cgtttcactt cccctaccca
     5041 tacggttttg gcttttcaac aagcgctgaa ggaactagag gcagaaggag gaatcgaaac
     5101 tcgatattca cgttatctcg ccaataatca tgtactacga caaaaaatga cagagatggg
     5161 gttccagcct ttcattcaag aatatcaggg gccattcatc acctcttttc tttaccctaa
     5221 ggactggcga tttgatttta atcattttta tcagtattta aagcaaaacg gatttgtgat
     5281 ctatcccggg aaaatcagcg atcatgaatg ttttcgtatc ggaaatattg gcgagatcta
     5341 tccggaagat attttggaac tgacagcaat tatcaaagac tataaggaga acgttaatga
     5401 ttaaaactgt aatttttgat tgggcaggaa caactgtgga ctttggctgt atggctcctg
     5461 tccacgcttt tagaaatgct tttctagaaa aaggaatcca actgactgac aaagaaatcc
     5521 gcgaaccgat gggaaaatta aaatgggatc atatccaaca gcttctttcc ctcccttctg
     5581 tcaaaaaaca atggatagct atttatggac aactacctca ggaaaaagag gtggaggaat
     5641 tgtatcaaca atttgaacat tatctgttta aagatttggt caagcattct accttgaaac
     5701 ccgacacatt ggaaacgatc gaacggttga gaaacgctgg gatcaatatt ggttccacga
     5761 ctggatttac tgctcctatg atgacaatcg tagcagaaac cgcaaaaaaa gaagggtatt
     5821 caccagactt catggcaact tctgaagacg tcgaagggta tggtcgaccg tttccttata
     5881 tgatccataa aaacatgcag tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg
     5941 gagatacggt ctcagatatc caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa
     6001 ttgaaggtag ttcgctaatg gggatttctt atgatgagta ccaaaaatta gagatgggga
     6061 aaaaagatcg tttaaaacag tctgttgctc ttagattcca acaagctggc gctgatattt
     6121 gtgtaaacaa tttaaaagag ttaacagaat acattttgta cgctgacaag aatccagaat
     6181 gacttttata caaaatcaga ggtcgtgaaa aagctgtact gcatcaagta ataagaacag
     6241 ccaaacaaaa atagctcctc atatttttcg tttggttagg cttattaccg cagatgcaag
     6301 cttttgaaca ccgtttattc gaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc
     6361 cccttattta caaacaaaca gtgtttaaga gacaacctct ccactaaacg gtgttcattc
     6421 agctgactct cggtatttgc tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg
     6481 tttctctaat actcggatca gaacgctgat ttcacattct ctttttctta catcttatct
     6541 ggtgcatgaa gtcctagcaa acgaaggtct tctttcaata atacagtcac tgcataaact
     6601 agagctaagc gtgcttcttt ttgttcatca tcagctaaaa tcttcgtatg agcatagtat
     6661 ttgttgaatg cttgtgctaa tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt
     6721 tccccagcag acaagaccgt ttcagggtat ttttgaacta atttaaccac ttcccagctg
     6781 gtatcgtcat tcaatgcata gtctgctgtt tctgaaggga caaatcctgc tttttccaac
     6841 aggctgactg cacgagcatg tgtatattgc acataaggtc cagtttctcc ttcaaatcga
     6901 acaacttctt ccaggttaaa gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa
     6961 atgactgccc caaccccaac ttgtttagct acagcatctt tgttttccaa atcagggttt
     7021 ttctcgctga tttgttcttt agcagattgg attgcttcat ttaatacttc ttctaataag
     7081 acgatctttc ctttacgagt tgataatttt ttaccacctt gcgtgatcaa accaaatgga
     7141 atatggcgca tatcgtcaga ccagtcaaag cccatttcct tcaagacagc tttcaactgt
     7201 ttaaaatgat agctttgttc attccctact acatatagag attgtttgaa gtcgtaagta
     7261 cgtttacggt acaatgcagc agctaagtca cgtgtaatgt atagtgtcgc accgtctgac
     7321 ttcttaatca acgctggatt caggtcataa gcagataggt caacgatctc tgcaccttta
     7381 tcctcgttca gcaaatgttt ttcttctaaa agtttgacga tttcatccat cttgtcattg
     7441 tagaaagctt caccgttcaa cgaatcgaaa cgtacttcca gcagatcata gattttattg
     7501 aattctttca tcgattcatc acggaaccat tgccataatt gaatagcctc ttcatcgcct
     7561 tcttccagac gtttgaacca agcacgagct tcttcattta attctggttc tgtttcagct
     7621 acttcatgga attggacata taaacgaagc aattcattga ttggttctgc tttaacagct
     7681 tcttctgtac cccatttttt gtaagcgacg atcagttttc caaactgtgt tccccagtca
     7741 cctaaatggt tgatgcggat tggctgataa ccgatttttt ccataatgaa accgatcgaa
     7801 ttaccgataa ctgtcgaacg caaatgtccc attgagattg gtttggcaat attaggagaa
     7861 gacatgtcga ttggtactgt tccttgattc ccgatattgc tatccccata atgttctttt
     7921 tctttcacta ctgtttgcaa aacttttttg ctgataagtt ctttattcat gaaaaagttc
     7981 aagtaaggtc cgacaacttc gattttttca aagttggcag aatcgatttt ttctgctaaa
     8041 tctgcagcga tttgctgtgg cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat
     8101 gcaacatctc cgtgttcagc tgattttgga ttttctagta attgttcgac ttgttccagt
     8161 gttaaatcat ctttgacagc gtcatagaca gcttttgcga caagatcttt attattcatc
     8221 tttattcctc ctaaagtgct cccttgattg ttaaacaaaa aaaatctcta gcgacggcat
     8281 acttagtatg ccatcactag agacgagatt tcccgcggta ccactccaat tgtcctaaaa
     8341 aaggaccagc tccatctgat atcggtagac gaccgtctgt ttaaaacaga attctccaag
     8401 tgcgcttcgt tgtatcttct tcattcggct tccaccactt ccgaattcgc tagccaagaa
     8461 tgatacaagt actctcttgt tcaacaaatc ttagtcttat taaactttct tgaattatat
     8521 cagaaaaaaa aacgaaaggc tagtcgtttc tgataaaaaa acaatccaga tggcctaaag
     8581 gagaagaaat accacctgga tagctataaa tcatttcgta aatacgaaag acttaacaaa
     8641 ttaatcagct acgataatcg ttccttcttc agaagctaaa agattctcga tattttctaa
     8701 agaagtgatg attgccttag cgtttggttt tgcttcaaca aatgcaatag ctgcctctac
     8761 tttcggaagc atgcttcctg gagcaaattg cttttcatca atgtattgtt tcatttctga
     8821 aacagtaaca gtttctaatt tcttttgatc tggtttttgg taattgacat acacattgtc
     8881 tactcctgtc aatacgatca gcaaatccgc atcgatgatt tcggctaatt tttctgaagc
     8941 aaaatcttta tcgattacgg cttctcgtcc ttctaatcca gtagctgttt ctacaacagg
     9001 aatcccaccg ccaccaacag agactgtgat aactccttga tcgaccagcg tttcgatgac
     9061 acgtgcttct ttaatcgaga tgggtttagg ggaagcgact acttttctcc atccacgtcc
     9121 agcatcttct ttgaatgtca cagtggagtc agcattcatt tgttctttgg cttcttcttc
     9181 cgtgtagaat ggaccgactg gtttacttgg atttttgaat gaaggatcat tttcatccac
     9241 aataacttga gtgactaaag aaaccacatc tttatcgatc cctttttcct tcagtacttc
     9301 gcccatggca ttttgtaacc agtaaccgat agatccttct gtcattgcaa cacatgtatc
     9361 taaaggcatt gccggtgttt tctcagaatc agcagcttgc tgctgaatca ataggttacc
     9421 gacttgcgga ccatttccgt gggagatgat caattcatct ccttgttcga taaatttcac
     9481 taaatacttt gctgtttcca ttaacgcttc ttgttgggct tttgcacttg catccgtaga
     9541 caagattgca ttacccccta aagcgacaac tacttttcgt ttggccattt cattcgtcct
     9601 ttcttcagtt ctaccgaatt ttctagataa caaaacgagc cagcgttttg tgattttccc
     9661 tcaatgctga ctcgtttttt cgtgagatga atgtggttat gcttaaacgc gagggataaa
     9721 caagttacct aatgttgctg ccatgatcgc tttgattgag tgcatacgat tttctgcttg
     9781 gtcaaattga cgagcatatt tgctgcggaa gacttcatct gttacttcca tttcagtgat
     9841 gccaaagcgt tctttcattt gttcaccgta aacagtgttt gtatcatgga aagctggcaa
     9901 gcagtgcaag aagatcaaac gatctgtgtt gtgtgttttt tctaccattt ccatattgat
     9961 ttggtaaggt ttcaacaatt tgatacgttc ttcgaattta tcttcttcac ccattgatac
    10021 ccatacatca gaataaagga cgtctgctcc gtctacacct ttatcaacat cgtcggtaat
    10081 catcaattgt gcaccggatt tttcagcaaa gccttctgcc attttgacga tttcttcttc
    10141 aggttgtaat tcttttggtg caacgatacg catattcgct cctaaaattg cacctgtgac
    10201 taataaagag ttagccatat tgtttcgacc atctccgcag taagcaacag tgataccttc
    10261 tactgttcca aagttttctt ggatagttaa aaagtcagcg atcatttgtg ttggatgcca
    10321 ttcatcagtc aagccattcc aaactggaac accagagaat tctgctagtt cctctaccat
    10381 tttttgactg aatccacgga attcgattcc atcaaacata cgacctagta ctttagctgt
    10441 atcttctgtg gattcttttt tacctaattg gatatcattt gcacctagat attcaggatg
    10501 tgcacctaga tcgatcgctg cagtagtaaa tgcagaacga gtacgtgtag atgttttttc
    10561 aaataaaagc gcaatatttt ttccttctag atagtgatgt gggataccac gttttttcaa
    10621 gtctttcaag tgttctgaaa aatcgatcaa atattgtaat tcttcttttg taaagtcttt
    10681 ttctgctaat aagcttcttc cttggaatac tgattctttc atattaaaca ttctccttta
    10741 gattgttttt atttaagatt aaagaggaaa tcccgacggc agctgacagt aagaccgccg
    10801 tcaggaaaca aattacttca agtcttcacg gacaagtggt tggctcatgc agcgtggacc
    10861 accacggcct cgtgacaatt cacttgaatt gatttctaac actttgatgc catagctacg
    10921 aagcaattcg ttcgatacat aatttcggtt ataagtgact acaacacctg gagcgattgc
    10981 caatgtgttt gatccatcat tccattgttc tcgaggtgca acgatctcat ctccattacc
    11041 agttgggatc agtacaagat catctaatcc aagagcagct ttcatcgttt cgtgtaagtt
    11101 atctgaatga gtaatcttga tatcatcacc atctggttcg attgtaaaga catccatctt
    11161 accgtcttta ctttgaattg ctggatggat agtgaattta tcgtgatcta ccattgtaaa
    11221 tacggtatcc aaatgcatca ttgcacggtt gtttggaatt ttgattgcta acactttttc
    11281 aaatcctgaa ttttttgcga ataggtggcg tgctaatttt tctaaagctt tcgcattagt
    11341 tcgttgagaa atcccaacag ctactacttt atcgcttaag atcaattcgt ctccgccttc
    11401 gatatgatct ggattttcac gatccaacca tacttcgatt cctttatttg caaatcttgg
    11461 atgatgtttc aaaatatatt ctgtaaacat cgattcacga cgacgtgctt caaaagtcat
    11521 cttattgact gtcatcccat ttcccattga tgcggaaggg tcgcgtgtaa agtacaagtt
    11581 tggcattggg tccatgtaaa atggatagtc atcgtctgct gacagatcgt atagactgct
    11641 agaacgtact tcaatatctt ttgtacgaac cccagccatg attttatcta ccatttcttg
    11701 cgtttccatt gatagaagga actcatgaag accctctctt acagcattcg atgcaatatg
    11761 agattcatcc aacattttat tcaagaattg ttctttgaca ttgcctgcgt caatcgcttc
    11821 tgccgctaat ttttccaagt aaagagtctc gacgccttca ttttgcaatg tctttgcaaa
    11881 gttgtcatgt tcttcttgag caatcggaag atatggaata tcatcgaaca acagacgatc
    11941 cataatatct ggtgtcaaat tttccacttc ttgtccaggc cttttcagta aaactgtctt
    12001 taatttccct atttcagaga aaacgtgaat aggtttatcc atgttgtgtt gcctccttct
    12061 ttttgattac atgtatagga tatcctcctt ttgtaatcac tttcataatt ttgtctcatt
    12121 caaaatgaga cattcctcac attaatattc gataattaaa taattaaacg tttttctatt
    12181 tttaagcgta atttattcat tttttattct gtatacactt tcaaaaaaat atttattcat
    12241 tgaaaaaaat tttttgaata aattaaaaaa ataaattata ttagcgttgt tagcgtttac
    12301 tatatgtatt attgttgttt tttatacaca aatcattcgc ttcaaaacaa attattcaat
    12361 accattgtat attttttaca taataacaaa ttatatttgt aaagtattaa aacttgtttc
    12421 taatgttggt atgttaaaac tttacaaatt ctttaaaaaa agtgacaata aatatagtga
    12481 acaataagga gaggactttt taatgcgtgt taataaaatc gattttcact tttcaaaact
    12541 cagatatcaa cctgattttg aacatttctc ggatgaagag ttccaacaga tcaaggaaca
    12601 tactgttctt cgttcttata aaaaaggaca gatccttttc gacgatggtg atcccagaca
    12661 acggttgtat atcttgacca aaggactcgt tcgtttagaa cgttatgatg aaagtgcaac
    12721 ttattactac tatgactatg tgaatacaaa taaccttttt cctatgtcag gaatgtttgg
    12781 agaagagacc tacacttata cagctcaagc gatgactgat attgaaacat tctaccttcc
    12841 tgtttcctac tatgaaaaat tagcacgaaa aaatcccgaa cagttgatct atatgataca
    12901 aaaaatttct aaagtagttg aaatacatga attgcgtatc caaatcggat tgacctctag
    12961 cgcctttgac cgggtaaaac aaaatttatt cattttgaaa gaagaattgg gcgtaccaac
    13021 aaaagatggt catatcacca ttccttatcc gatcacctta aaagaattag cagtgaatag
    13081 cggcacgaca cgtgaaactg ctggacaagt aatcaaacaa ctacgtgatt ccggactgtt
    13141 agattatgac aaaaagcgtt ttactttctg taagacagct actactccag ctatgagaga
    13201 ataacgcgaa caaaaataag cagtgaccct gatatttatc agggtcactg cttattttcc
    13261 tccctaaaac ttctgaaaga tccggcagtt ttagatcact gggcaagact caaagatttt
    13321 ctatgtgtgt ttcagttatg taatattcac tgtttgcaaa aaatactgat agattaacta
    13381 gaggaagcgt cttgtcctca tccgacctag attattcgat attcctcgac aataaacgat
    13441 taatcagtga gggcaatcag aggaacggat aatgatgaat aagctgtaaa aaatagggct
    13501 ttgacaaaag cgtgtcacag cccttatttc gattaaacgg tgtgtacctg tcaactcttc
    13561 ggtcacaagt cataatgttc ataaacatga ggagctgttt tttgaaattg ttccttgtga
    13621 atcagagtta aacgacaggc ttacgaactc ttgattaaac ggcgttcaaa agcttgcata
    13681 cgcggtaata agaacagcca ggtcaaaaac atgagaagcc gtttttcctg ggctgttctt
    13741 attacttgat gcagggcaga tttttcacaa cctctttatt cagataaaac caagcttgga
    13801 tcagcattca actgatagct agcaaaattt cttttttgca tttccacatg tgctgctgct
    13861 ccaatcattg cagcattatc tccacaaagt cgaagagggg gaatcactag ttctacctca
    13921 gggagtttag ttgccaaagc atcctgcaat ccttcacgca acccttgatt ggccgctaca
    13981 cctccagcta caatcagttg cttgactgga taattttgac aagctcgaag tgttttagta
    14041 atcaatacat cgatcacact tgcttgaaag ctagctgcta aatcattttt gtctagatct
    14101 tctccacgct gttgtgcatt atgcaccaaa ttgataaatg cactttttaa tccgctaaaa
    14161 ctgaaatcat aattatcttc atggatcatc gctcgtggaa aatggtaatt atcttttccc
    14221 tgatgagcta gctgatcgat ttcttttccg cttgggtaag aaagacctaa tactcttcca
    14281 actttgtcat aagcttctcc tgctgcgtca tctcttgtct ccccaatgat ttcatatgat
    14341 ccatcttctt gcatatagac taattctgta tgtcctccgc taacaagaag tgccatcaat
    14401 ggaaactgga atggtttaac cagacgggct gcatagatat gccctgccat atggtttaca
    14461 ggaataagcg gcaattggtg tgcccaagca aaagccttag ctgcagaaat accaatcagc
    14521 aacgagccga caagtcctgg tccataagtg acagcgactg ccgataaatc ttctgccgac
    14581 acaccagctt ctaccagtgc atcttccaag cataaggtga tttgttctac gtgatggcgg
    14641 ctagctactt caggaactac gccaccaaaa cgtttatgac tgtttatttg agaagcaacg
    14701 atgttagata agatctctgt tccatttcta acgaccgcta cactcgtttc atcacaactt
    14761 gattcaatcg ccagaatcaa ttcttcattc atttcatcgt ctccgttttt aactttctgc
    14821 tcataatgat cgcatcttcc gtcggattgc gataatatga tttccgttta cctaatatac
    14881 gaaacttttc tgattcataa agttttcttg ctgcttcatt agatagacgt acttctaaaa
    14941 aaacatgcag cgaatctttt atttgttccg taatcagtaa ttgtaacaaa tgccgtgcat
    15001 gcccttttcc ttgttctgaa actgcaacag caatatttgt gatctcgact tcatctatta
    15061 ctttcgagta tccaataaag ccacagatct ggttatcttc aactaaaagc agataatcag
    15121 agtgaggctg ttggatgtct gccaaaaact gcattttcgt ccaaggtgaa ccatgtggat
    15181 aagcattttc gctaatcatc catacgcatt ctgccaagta cgcttcatca aaatcttttt
    15241 ttctgcaaat caactaatct cactcatttt ctaaaagttt tatcatatcc aacgcatctt
    15301 cttttgtttc agtgtaatat ctttttttga tcgttgttgc ttgaaaacct aacttccgat
    15361 aaagtctttg tgcatcttga ttactcatac gcacttctaa tgacatcgtc tcacagcgat
    15421 tcattatagc aaagttctcg atttcgtcaa taagcagcga accgattcgt ttacgctgat
    15481 atgccggtcg aacggcaata ttcgtgatat ggcaatctaa accgaacgct cttgaaccga
    15541 taaaaccaat cattcttcca ttatcaaaga tacctagata tagatgagga agcggtgact
    15601 tcaattctga tataaaggca ctttttgtcc atggcaattc gccattatag acttctcgtt
    15661 cgacatccag cagctctttg atatcttctg tagtcaattc tcttagtacg taacgacgat
    15721 cgatttgttc gattgttttt tctgtataaa tcattttatc ttgaaaaaag cttctggcat
    15781 acgctctaaa ttttttcaac ataactttcg tctccaggtg tgtgcgtctc caaccatttt
    15841 tcttctgctt ctacccgttt taaatagcga ggaagaaaag cttgtttctc tgcaactggt
    15901 tcagctaaag cacctaattg agccacagtt acaccacttg gaatatccca tgctggaact
    15961 gtattaatca tcgcactagg caatatttcc tttatttcat agaagaattt gtgacaatca
    16021 ctcccgacga aataaatagc ttcattcagc ttttgtagtt gtgtgaacaa atcagaagca
    16081 gaaatatgta catcttcaag tacaatttct aatctatttt ctttccagcg gtatgcccca
    16141 gcgtatacat tttttcttcg agcatcaaac atgggcacaa taattttatc cacaccgatg
    16201 caattagccg cgatggtttt cagactagat actcctacta gctctttatc taatgtataa
    16261 gcaatcgttt ttgctgtcgt tacacctata cgaagccctg tataagaacc gggaccatca
    16321 gaaaccgcga ttcggtcaat gtcttttgga cttatttgaa gatctgcaaa caattggtcg
    16381 attgcaggca tcagtgttac actgtggttg cgtttgatat ttgtttgtat ttgtccaaga
    16441 atttgttcgt cttctaccac accgatcgcc aaagtttggt ttgcagtatc tattcctaat
    16501 gtaatcatat atttgatcct ttctgctatg acattctcgc cttccattgt agcatacttt
    16561 tttattctga ggatgaaaat accaaaggca aaactagtac aatgatacat gattatctgc
    16621 tggcgggcaa attttgcccc ttcctgaaac acaaaaaagc tgcggtatca cttcatttcc
    16681 ccacagcttt tccatctttt atttcgttga ttctcgaata atcaactctg tttccacaat
    16741 aatctttctt gaaaccggtg ctttggaatt acttaattga agaatcgtct ccactgctag
    16801 ctctcccatc cattcggtat aaacttttac agtagaaaga gagggcgtta catatttcgc
    16861 tacactgaca tcattgaaac ccatcacaga aatatcttct ggtaccacta aacctttttc
    16921 ttgaatcgct ttcagtgcgc caatcgctaa tgcatcatta gctgcaaaaa gcgcatcagg
    16981 aacatgcgga ttcgttttta aatactgctt cactgcttga tatcctgact cgacagaaaa
    17041 atctgcttcg ataattggac cttcgtcgta gcctttttct gccaaaactt gttgaaatgc
    17101 cataattctg ggatctttta gccgctgatg gtttttcttc gtatattctg ttccagcaag
    17161 catagcgata tgttgcgctt tatgggagac aaaatgttca atgacttttt gaacacttga
    17221 cttaaagtcg acaactagag agtcgattcc ttgagaagtg ccgtcagaat ctaccaaaag
    17281 caaggcattt tgggatcctt tcatttctaa taactgagct tcgtcgaatt ttcctagtgc
    17341 gatcgttcca tctgtcggaa gatcactaat ggaatcccac gtttctttca ataaagtaac
    17401 gtttgcttct tctgcctttt tttcgattcc tagacgaata gataaataat aaagatcttc
    17461 aagctcttcc gtatcgtcgt accactggat caggcgaagc gtttgcttag tatttgctgc
    17521 atttttacga tgtttcgtat aatttaagcg ttctgctgct tcaaaaattt tttgctttgt
    17581 ggctaagcca acggaaagat caggatcata gttgagaact cttgaaacag tagcaggaga
    17641 aactcctgcc aactgcgcaa tgtctttaat tgttgccatt tgtctctcct ccttttttac
    17701 gtttataaaa ttgcagtaaa tcgttcaaac gctgcctgtc ctttttcatc tcgcttgaat
    17761 actccagcgt cttccagtac acgtgcaaaa acctgaccta cagatttttg tacgacatcg
    17821 gtgaccgtat ctttcgtcaa atcaggatgt tcttttttta attgttctgc ccacggacga
    17881 tgatattcag ccatctggtt ttcttctcct aataagaaat gctctacttc tactagttct
    17941 ggtttcaaac gaggtggtaa aacagcaagc cccattactt cgatcaaccc aatgttctcc
    18001 tttttgatat gttggacatc tttatgtgga tgaaaaatcc catcaggatg ttcttctgaa
    18061 acattgttgt ctcgaaggac aaggtccaac tcaaagtaat tgtcccttct tcgtgcaatt
    18121 ggcgtaatcg tatggtgtgg tgtaccatct gctgaaaaag ctctgataga tactgtttca
    18181 tccgaatatg ctttccactt ttccaatact gagttagctg cttcaatcaa caattttttc
    18241 tctgttgatt gcaaacggat cacagacatc ggccatttca cgactcctgc gatgacttgc
    18301 ggataatctg ctagtttgat aagtttttct atcggtgctt ttgccatagg aaattcatga
    18361 cgtcctgcct gataatgatc atgagaaaga atcgatcctc caacaatcgg caagtcagca
    18421 ttcgaaccaa caaagtaatg aggcaagact tcagtaatcc gaagcagtct agcaaatgtt
    18481 tcctttgtga ttttcatcgg gcggtgttgt tctgataaaa tgatcgaatg ttcattgtaa
    18541 taagcataag gagaatattg gaatccccag ctttcaccgt ctagattcat tcgaataatc
    18601 cggtgattgg tgcgggctgg atagttcaag cgacctctat atccttcgtt ttccatgcaa
    18661 agcatgcagg taggatagtc tacttttacc gcctcgcgtt ctgctgcgat ttgttttgga
    18721 tctttttctg gcttagataa attgattgtg atttccagtt caccatactc cgactctgcc
    18781 ggaaatttga tgttttttgc gatggctctt gttttgatgt agtcattttt cttacttaaa
    18841 ttaaagaaat actcagttgc ttcctctggg cttttcgaat agtgctgtgc aaagaatgca
    18901 ttgacgactg aaggtggcgg tgtgagaaaa tccattaatt gcgcttcaaa aatctcttgt
    18961 tctgctggca attctgaaag aacaccattt tctcttgcct tagcgacaag ctgatccaat
    19021 aagtctacag attcttcctt aactggtctt acttcaatgc tttctagagc atcttctcct
    19081 accatcccta aaagacgatt ctgtaaatat aagcggtcca tttccatcca tccgccagct
    19141 tcgatcgcaa gtgtggcaaa atcacaaatc gtttgactga ttgacatatc tcattcccct
    19201 attcttcata accatttgga tggctgacat gccaatccca tgctgttttg ataattgctt
    19261 tgatatctgt gtattcaggt ttccaaccca gaacctctct cgctttttca cttgaagcga
    19321 ctaatcggct aggatctcca gctcttcttg gtgctatttc tgctggaatt tcttttcctg
    19381 tcacttctct agctgcttct agcatttcct tgactgagta tcctttgctg ctgcctaaat
    19441 tgaagaagtt gctttcattt ccttctttca agtattccaa agctaaaata tgtgcagcaa
    19501 tcaaatcttc tacttgaaca taatctctga tacatgttcc atctggtgta tcataatcgt
    19561 ctccataaac agccaatgct tttctttgac caagagctac ttgtaaaatg attgggacaa
    19621 gatgcgtttc tggtgtatga tcttctccga tagatgcatc tgcttttgct ccggctacat
    19681 tgaagtagcg caaagcgaca tagcgcatgc catatgcttg gtcacaccac ttcatcattt
    19741 tttccatcat caatttactt tcaccatacg gatttttagg attagctggt gtatcttctg
    19801 tgattggaga ctcttttggt tctccgtaag ttgctgcagt agaagagaac acgattttat
    19861 tcacattgaa ctcgtgcatg acttcaagta agatttgcat accgtatacg ttgttgttga
    19921 aatacatcaa tggtttttcg acagattctc ctactaaaga gcttgcagca aaatggatta
    19981 ctccttcaat cggttccttt tcaaatacgg aacgtagaaa ttctttatcc cggatgtctc
    20041 cttcataaaa atgagcatct ggatgtactg cttgtttgtg acctgtgagc aaattatcta
    20101 ccacagctac ttgatacccc ttttgaacta gctggtctac agcgtgcgaa ccgatataac
    20161 cggctccgcc tagtactaaa attgtcataa aaaacactcc tttgattagt aaaattgtgt
    20221 ttgttgatta tatataattt aaaaaaacga agtttacttt tatggtgaaa acaaacaaag
    20281 taaaatttcg taaattcata tggtaaattt ataataaaac atttagtaaa cattttcaag
    20341 gattttttgc aaaggtttac aaacaaacgt aaaactagcc tttttttctc ttttgttcca
    20401 taatagatat aacaaataat aaatgaggtg gattcaatga aaaaatttgt atctggaata
    20461 ttagtaggaa gtctggcaac agccgctgcc gttgctggtt tggtcgcttc tgtgaaaaaa
    20521 acagtgatcg atccaattga cgaaaaagaa gcaatgatcg aagaaaaccg taaaaaagca
    20581 atgcgcaaac gcgtttctcg ttaataatta tttaaaaaca cgcatgaaga cttgtgaaag
    20641 aacagataag tcatcatgcg tatttttatt tgtcagtcaa tataagttgt gctattttat
    20701 ctgcctgtct cagattccat ggatctgtat aaggtctagc tactgctgca gttagccaat
    20761 ccacaccttc aacaggtatt ccatagcaat aaaagtcaga ctggatttcc cctgaagcat
    20821 caatcacgcg attcgtacct cgctcaacat ctatagcttc tgaatgataa cttcctgtat
    20881 tatctgaaaa tgaaaaaggg cgaacaagtt tgttttcctt caaatttttt atgacaggat
    20941 tacttgaacg agaaaagctt gtttgtggca atcgagcttc aatcaaatag cgactgctga
    21001 tttctttatc ttgtagagaa ataacatagc ggttgtgctt ttgctgaata tcaaatggcg
    21061 gttcgaacaa atgaaaaata cccgcatcta tcagtgctgc taattctttc gtccgaatga
    21121 ctggcggacc gatcgttaaa aagctattta gatgagtaaa ccatccccat agtatttctt
    21181 tgtattcctt tgcagaaaaa acgtgccatt caatagcctg ccggattgga tcacgccagt
    21241 cttttaatgc atcaaatgtt gaagcaaatg ctcctgtgca attgcctttt tccgcttctt
    21301 ttatctgccc ttcaataaat gctcggctag cttctgcaaa ggactcatcc tgataggtaa
    21361 gaggaatttc taaatacgac caatcccatc tataagggat tagtgctgga tgctttccga
    21421 gacactcctc tgaagagagg gagagaaaat catgctcaaa atttttcttt ggaatcggca
    21481 aatgatattc ttcgatgatt ttcttgtaat aaaacaactc aacgtctttt ttcagatagt
    21541 caaaaaatgt ttcgccagtc aaaaatcccg actgatgcca ttgctccagg ttttcttttg
    21601 ttagtaaccg tggccatgcc attgatccgc cttgcttttg gtttcttcct cgagggtaat
    21661 aagggagccc ccttcttgat ccactgtata caatcggttc cttgcctgat ggatgataga
    21721 caagttttcc tttttcgttc tcgaagcgcc cttctcttga tactgtaaac aatccgacat
    21781 aatcaaaaaa acttaagcct agacctctta aaaagacatt ttctttaggc ggtatcttcg
    21841 aaagatcgac atctgcagga ttagaaggaa gctgatacag taaatcattt ttctttgcgt
    21901 agtctaccca cactctttct tcttctgtct catcattttc ccagtgaccg cttgctaaaa
    21961 caagctgatc gatagaatag ctttgctgat ctgtctgtaa aagataacca tcttttcttt
    22021 tgctgacttt ttttactaaa gaataaacaa actgaataga agctggaaat tcttcttgca
    22081 actttgagaa aaaccagcgt tgatagagtc cgtataaaca tcgagtagat tgctgatctt
    22141 tttccagacg attgatttct tctataatta gttgcttatt tttggttacc gtctgttcga
    22201 tataatctgt agcaggtcct ttactccatt gaaacaagtt gggtccctgt gttaccaatc
    22261 ctccactgga caatgtttca tctgtaaaaa gcgtcacttg ttgagaaaca gaattcatta
    22321 ataactccgg tggctgattc aaccgccaaa ctcgacctcc aggaccattg ggatcgaaaa
    22381 gaaaaagttc aagagaatgt ctgtcctgat tattatggag aatcctttct gctatactta
    22441 atcctctagg tccggcacca atgatcccta ttttcatttg catgcctcca tttcgataaa
    22501 ttttttcaat aaaacagcat ggttctgatg ttcatttttt gcaccataaa caaaagtaat
    22561 gactggatgt tctttcataa gttgactgag tttaaaaata gcttgcttct tttcttcttc
    22621 gaaaatcaat tcttgctggt atttctttgc aaatgaagca aatcgttctg gtacatggtc
    22681 aaaagttttg cgtgcttcat tactaggtgc aatttctttc aaccacagat ctaactgcgc
    22741 tttttctttt gaaattcctc gtggccagaa cctatccacg agtattctat agccatcctc
    22801 tttttcagca tcaaggtaaa cacgttttaa tttgatcata gttgtttcct tctttctgcc
    22861 ttttcgctta gcttatatat gtagataata gcacaaaata aaaaaaatga acggcacaac
    22921 tatccattgc gccgttcatg tgatcttggt caaaatctaa tgattgattc ggtttttaaa
    22981 gctgtcatac aatcatctgt tttatacttt tttacttttg atttttccag accatccgtc
    23041 atatccaccc ttaagtaaat aaatctcttg ataacctgct ttacgtaatt tatttgctgt
    23101 acggatgctc aagcttttct tttgatcgta aatatagaca ggctggtctt ttcgaataga
    23161 tcctaatgaa tctttcaaaa cagtgtaagg gatgtttctt gcacctaaaa tatgcccagc
    23221 atcaaacaca tctttttcgc gaacatcgat tacttgtgct tttctcattc cttctcgaaa
    23281 ttcttcttct gtcaaagtcg ttgctgaacg cttggccatt actcgtagat agacttccca
    23341 aaagatgatt gcaagcacaa tcaatagcaa aatgatatta attacccata aaaccatgta
    23401 atgcctccgt ttttttaagc aaatttcaag gctaatgagg cagcaccaat gacaccagct
    23461 tcattaccaa gctctgctaa tttgattttt gttgattctc ttacttgcgg gaaagtaaat
    23521 tctttaaaat atttctctac acggctacgt aaaaattctc ctgctgccga tactcctcca
    23581 cctaacacaa tacttgacgg attcaatgta ttccccaagt tggcgcaagc taaacctaag
    23641 tagaagcata cgcgatcaac aaccatcaat gcaaacggat cgtcttcttg agccatttca
    23701 aagatatctt tacttgttac ttcttcaccg ttgtctaaca ttgctttcag ttttgaatct
    23761 cctgcgtatt cttctgccaa gtaacgacct agacgtacca caccagtagc gctagacaca
    23821 gtttctaaac atccgcgttt accgcaagta cattcgaacc cttcaggatc cacagtgata
    23881 tggccgatct ctccgcctgc tccgctcacg ccgtgaatca aatttccgcc agcaacgata
    23941 ccaccgccaa cacctgttcc tagagtaacg aatacaacgt ctggatcgtt ttctccagca
    24001 cctttccatc gttcacctaa agcagcaaca ttcgcatcat tatcgatagc gaatttgatt
    24061 cctgttcctg cctcgatcag ttttttcaca aattgaactt ctgtccagtt taagttatac
    24121 gcaccaataa ccgttccagc ttcgctgtcc acgcttcctg gggtacccat gccgattccg
    24181 ataaagtctt cggctttcat attatataaa ttcaaacgat ggttgattga ttcaatgatt
    24241 tcaggaacga tatgcttacc atcatccaaa atattggtat cgatgctcca tttttgttga
    24301 atctctcctt ctggtgttaa aattgcaaat tttgctgttg ttccacctaa atcaatccca
    24361 ataatcttct tgtccattac aaatttcctt tctttcggct ttcttcaatt cggtgctctc
    24421 tttttaatat gcgccacgct gtcatgtatg tatcatgatc aatcaaacgg ctatcataca
    24481 gattacgcaa ttcgatagtc attaattcaa tgtcatagat gcgtgcaccc acatagataa
    24541 agatgccaaa ttttttgaac agctgctgta catcatataa gctctccatc tttaagcact
    24601 tccttcatat tatacaagta aaccatattt ttttaaaccc agtaaaatac atattacaaa
    24661 aagaaataca aaaactagtg tagaaagaat cctttggtgt atcgaatagc ttgtctgttg
    24721 tttcggtaga cccaaaacat tccctaacac taatccgcca attagcccac cgacatgtcc
    24781 ccaaacatct acagaggtat ccagaagacc aaaaacaaat gtcaacacaa taaataaaag
    24841 aaaatgtctg actagctgct ggatcgctgg gttatgttta aaatgaaacc ctaagataaa
    24901 caacgcaccg aacaaaccga atatcgaagt actcgcacca ccagacagaa cattcgcttc
    24961 gttaaaagca aagcttgctg tatttcccat tatcccgctg aacatataaa tcaagaaaaa
    25021 acgccaatgg ccatagattg cctcgatttg ctgccccata aaatacagta tcagtgagtt
    25081 cattacgaaa tgcatcagtc caaaatggat gaagattggt gtcactaaac gccaccattc
    25141 attgaagtga acaagataag gaccatacat tccggcataa taagggatct caagtcgcgg
    25201 aaataattcc atcatgagga agaccgctgt ctgtatccct aaaaaagcat aggtccataa
    25261 cggtcgtctt aaccatgcac gcaattttat ttgctgttga taattcatga aaacctccca
    25321 tttcatttta gtcaataaac aattgttgga tcttctgatc aaagctctcc ggttgccatt
    25381 gttcattcaa ttgttcacga aaaacaaggc tacatgtttt tttatgaaaa ttatttaaat
    25441 accggtcgta ataaccaccg ccaaaaccga ttcgataacc ggctctattg tagataatac
    25501 ctggtacaat gaggagatcg atcatctccg gcaaatagag cttctctgat tgtggctcaa
    25561 gaacaccaaa actcgaacgt acaagaacgg actgttcatc catttcataa aacgccatct
    25621 gacgtttcgg caaagtttta ggtacaacta ttttcttttg ttctttttct gctcgttgga
    25681 taaccggctg tgtatccagt tcaaattcat tcgataaagt cgtccctatc acttttgctg
    25741 acttccagta ctcgctggcg aatagctgtt tatagattcg ctgttctttt tcctttttct
    25801 tgccactttt ggccagattt ttcagctttt cgattccttt ccggcgtaat cttactttca
    25861 attggtccaa aaactcaccc ttccttctct cataaaatgg tataggaaaa aaagaaaaat
    25921 agcaatcgaa ctttcaaata aatgcataaa tagaataaaa agaagaccta gaaatgattg
    25981 gccttccttt tttaataaat ttcaactgtt cagcgcgtgt ttttgtctga taaaaagtgc
    26041 atacagaaaa tatggcgcac caaaaagggc aatcaaaatt cctgtaggta cttccccact
    26101 tggtagaatc actctagcaa ccatatctgc actaagcaga aaaatggaac cgaacaaacc
    26161 gctcagaaca aaggtccagt tattttcttt acgtacgact agcttcgcag catgaggagc
    26221 tagtagtcct acaaaagata aactacctgt caccgctaca ctaccgcttg ccaaagcaac
    26281 agctaacaac aaaaggattt tttgactttt agccacgtta aggcctaatc cttgcgcgcg
    26341 ttcttgacca aatgatagca atccaatcct ctgtgcataa agtcctccta gcggaattaa
    26401 gataaccgcc catggaagca atgcccaaac ataaggccag ctcgcacccc agattgatcc
    26461 cgctaaccat gcattcacaa atccataatt ttccttagat aattggatcg tgcatagcaa
    26521 tgttaatgca gaaaggccgg cattaacggc aataccggac aataagattc gattcatacc
    26581 caaaaagcca tttcgatcat aggccgcaag atagacacag caagcagcta aaacaccacc
    26641 tagaaaggct acaactagca atagaaacgg cagcgaatag tgcgaaaaga aacctaaata
    26701 aagcatcact aaaaatcctg ctccggcatt aatgcccaat atactagaat ctgccaattc
    26761 atttcttgtt actccttgca agatataacc agaaacgcca agacaagctc ctgctaataa
    26821 actgatcagt aatcgaggaa ctcgaaattc tctaacaatc agcgccaaag aagatgattc
    26881 tttccctatg aacgtggcaa aaagctgatc caggtaaata aaaggcgtac caatcattaa
    26941 actacctata gacaggatga caagtacaaa aagcaagaaa tagaacgtcc aatatatttt
    27001 tttcaccgtt gttccctcct tacttgtatc agtaaaaacg gaacgccaat caatgcaatg
    27061 actaatccaa aaggtgtttc aaaaggggga ttgatcatcc tcgctgtaaa atccgccaac
    27121 ataaccaatc caccgccaac taaaaaagag acaggtagaa cataacgata gtccgttcca
    27181 accattccgc gaacaactgg cggaaccaac agcccaataa atgaaacagg tccgactaac
    27241 gaaacggcaa tggctgctaa cactgcaact agacagaaaa caatcaaccg cgttcgttta
    27301 ggatgacttc caagagctaa tgcatgtgtt tcactcaaat gaatcaaaga tattgctttt
    27361 cctaatgaaa aagaaaccaa aacagcaagt atatataaag gaaacaatac tgctaattgt
    27421 ttccatgtga tattggcagc tccgcctaca aaccaaaaag caagatcctg ctgcagatta
    27481 aagaattggg taagtgcttg gctgatagca gtaaaaaaag aactaatagc tacgccagct
    27541 aaaatcagct gtaaaggatt catgcccatt ttcgaaaact gcaaaaaacc aaatacgagc
    27601 aaagttacag caaaagaacc tgccaatgac attagaaaca cagaaaaatc agaagcttta
    27661 ggccaaaaag caaaactaag ggccattgcc aacgaagcac ccgcgttgat tcctagtaag
    27721 cctgaatctg ctaaaggatt tcttgtcact ccttgcatca atgcaccgct caatgcaaaa
    27781 caactcccta caagaaatgc tcccgccaca cgaggcaaac gaatatttcg tatgatttgg
    27841 tgactttgtt ttgatctatc aaaatcaaac aacgcctgcc aaaaatctgc ttttcccaca
    27901 gtcgctgcgc catttgaaag agcaacaaaa aaaataatca aaagaacaaa acagaccaaa
    27961 agcaggagtc tatttttctt catagtcctc ataattgatc ctcctgctct tttggcaaca
    28021 aatcataggt tagaaaaact ggtttatttg ttcttggaag cgtgacaatc gttgcatcaa
    28081 tctcaaatat ttttttcata cactcttttg tgatcacttc ttcaggtgta ccttctgtga
    28141 tcaacttacc gtttttcatt gctaataaat aatctgaaaa acgtgaagca tgattaatgt
    28201 catgaatcga catcaagatc gttgtttgtt tcatttgatt gatttcagtc aataactgta
    28261 ggatctctaa ttggtgtgcc ggatctaaaa atgttgtcgg ttcatccaag atcaagatat
    28321 ctgtttcctg agcaagcgcc atagcgatcc agacacgctg ggtttgtcca ccagacagcg
    28381 ttgcgatatc ccgcatacga aatccagcta gacctgtagc atccaacgcc cactcgattg
    28441 cttcatgatc ttgcgtgtcc atgccactga acagtttttg ataagggtat cgtccgtaag
    28501 atactacttc ttctactgtc atacctgtag ggtgttcact agtttgtgct aataatgtaa
    28561 ttttttttgc aagttctttt gaactgtatg tagagatatc agagttattg acaatgatct
    28621 ttcctgattt cacaggtagt atacgggtaa aacttttcaa taaagtagat tttccacttc
    28681 catttggccc aatcaaacta gtgattttcc cttcaggaat cgtaacagac atgttttcaa
    28741 tgattttttt gttttcgtag ccggctgcaa tgtcagctgc tcttaatctc gtcatttcac
    28801 ccatcctttc gctgaaaatc attctcaatt atgacgctgt ttcaacattt cgtcaagcgc
    28861 tactttcata tctatagcca aatgaagcgg ttgacagcgt tcgaaatatc aggaataata
    28921 accaatgaga aagattctca attatgttag gaggaaagaa atgaaattaa aaacaaaagt
    28981 attcacatta tctagcatat tgctgctggc tggagtactg agcgcttgct ccccctcttc
    29041 agataaagaa acagctacaa ctgccaattc gacgacttca tccaatagta ctcatacagt
    29101 cacagataca cttggacata aagtagagat tccaaacgcg cccaaacgga tcatcggcag
    29161 ttacttagaa gactatctga ttgcactagg ggaaaaacct atcgcccagt ggactgttgg
    29221 cagtggttcc attcaagatt atctgcaaga tgatttaaag gatgtcccaa cgatttctta
    29281 tgaccttcct tatgagaaag tgttaagctt tgagcctgat ttgctgttga tttcttcttc
    29341 cgctactgtc gaaggcggga aatacgaaca atatagcaaa atcgcgccaa cttatgttgt
    29401 aaaaaacgga acagatgtca catgggaaga acaactaaaa gatgtcggca aagctttggc
    29461 aaaagaaaaa gaggcagaaa aaatcatttc cgattatcaa aaatccatca agaaaactcg
    29521 agaagaacta gcagataaaa tcgagaacaa gacagctgca gtgttgtggg tgaccaacaa
    29581 ctcagccttt atggtcagca aaaatcggtc aagtggaaga atcgtatacg acgatctgga
    29641 atttggtgtt cctgatctag tggaagaggt ctccaaaaaa gccacttctg actggtctgc
    29701 tgtttcctct gaaaagctag ctgaattaga tgctgactac atcatccttg tgaacagtga
    29761 caaagatgct gccatgttca atgaaccaag ctggaagaat cttaaagcag tgacagacaa
    29821 tcatgtactg caattcggac ctgaatctag ttggctatat aacggaccta ttgccagtca
    29881 ggaaatggtc aatgatatca aagaaaaact aaattgattt taactaaaaa aagaatcttc
    29941 acgattagtc gtgagatgct ttttttctta ttaacaaact agacgcatag attttattct
    30001 tcttttgaag caaccctatt ttttactctt tcccattttt gaaaacgatg ggcatataga
    30061 ttttcttgat ccgttatccc ctcatactcc tctataaggg cgaagtctga aaaatcaata
    30121 ttcccaatat acgtatcgcc ttcaaacgtg ctatcgatca atgttcgcca gatccgacca
    30181 gtgtaaggaa gaagttcttg aaaaatccga ctaccacccg aaacatagat atcttctgag
    30241 atcgtttgac tatacgacaa aagttcatcg atcgaatgca ttacttccgc attttctttc
    30301 gtgcgaaaat ttggttgcgt tgtcaacacg atgatatggc gataaggaag tgaaagatcc
    30361 cccattcctt cataggtctt tcttcccatc actaaaattc gatccattgt atgttcgcga
    30421 aagaaacgca tatcatttgg caatcgccat ggtaacaaac catttttgcc gatcagacca
    30481 tttttatctt gtgcccacat ggaaataaac atattatccg ctccttatga ttttagatgt
    30541 tttgagataa aaaaagtctc aattctttga ccagcaagga attgagaact ttaaaaacat
    30601 tattttgttt cacggtgcaa agtaactttg cgttcgcgtg ggcaatattt ttgtttttcc
    30661 aaacgatcag gattgttacg tttgtttttg cttgttagat agttacgttc tttacaagaa
    30721 gtacactcta aagtgatgtt tacgcgcatg tttttccctc ccatttttac aagaatcctc
    30781 taagatgact ctcagacttg attatcttac catgattttt tctagattgc tagtgttttt
    30841 cacgattttg ttaagtaaaa ctacttgacg ataaatacaa cggttttact tctcaaacaa
    30901 aatctttttc tttttgtata aataccctta cacttgttta tatagattat gttttttaat
    30961 aaagggggat actaatatgc catggaacat gaaggacttt cctgcatcaa tgaaaaactt
    31021 agataaattg acaagaaaaa aagcaatcga catcgcaaat gcattattag atgaagggta
    31081 ccctgataac cgtgcgatcc caattgccat tgatcaagca aaaaaatggt atgaagatgc
    31141 tagtgaatca gaaaaacgaa ctttcgaaaa agaaaaaaat ccaagcaaga cggatgaaca
    31201 cgatacgaat cctcgtgctg gaaaactgct tgattcagat gtgatcgtag aatatgagga
    31261 agaacaatgg atcgtgaaat ctaaaggggc aaaaaaggca agcaaccatt ttgatacaaa
    31321 aaaagaagca atcgaaaaag gaaagcaagt agcacaaaac aaagaatcaa ctttgttcat
    31381 ttataaaaaa gatgggacaa aagaaaaaga aatcgattat tgaacatatg taaaggggtt
    31441 gtgacgaaaa tcgtgtcaca acccccttat gttcgaaaca atggtgttcc tctgccaact
    31501 ccgaaccata agtcataata tctataaaac gggaagagct gttttttgaa attgtttctt
    31561 atcactcagt gttaaacggt aggagaaaac ctctttttta ttgatatttg attttcatca
    31621 aacagtcaca tagtcgattg cttctgcttg cggaatcgtt tcatctacaa agtagacttt
    31681 ataccatgtc aagaaactga agattgtcca gatttttctt tgctcttctt tacgtccttc
    31741 gtgatggtca tctagcaact tcatgatttt ttcttgatca aaaaattctt ttgcaaaatc
    31801 tgcttcgaat aatgctttga cttgttcgta gccgtgttct tctttcaacc aagctttgat
    31861 tggcacaggg aatcctaatt tcacacgatt cgaccattct tccggaagat gtttgttcgc
    31921 agcttgtctg aaaatatctt ttgtgttgtt ttgattcaat aaaaacttac ttggtatttg
    31981 ttgagccaat ttcatgactt cgatatccaa taaaggtact cggacttcca aagaacttgc
    32041 catagaaagt ttatctgcct ttaacaagat atcttttggc atccattgat gaagatcgac
    32101 atattgcatc ttattgactt catctttgat tccttctgtt ttctcgtaat gaacagtcat
    32161 gatttcatcc acagatggtg ctgtttgata ttttggtgtt aacacttctg atgcttctga
    32221 ttcttcgaac actcgagcat gaccgataaa atattctttt gcaggagcaa gtgattcata
    32281 taaatggatc cgtccatgga aatttttcat ttttccgatt ttacgtccta aattatagcg
    32341 tgttcctttt ggtaattttt tcaatccttg agcaatcaca cggatgaatt ttgaattcgt
    32401 atggaatcca tacgtttggt atcccgcaaa cagttcatcc gccccttctc cagattggac
    32461 tacccgcaca ctttgtgaag caagatttgc taaaaagtac aaaggtacgc aagaaggatt
    32521 tgaatcaggt tcatctaagt gatactggat caaagggaat gctttgaacg acatgtctcc
    32581 gtcgatcact gctgctgtat tatctaagtc caatagttcg gttagttttc tggcttcaat
    32641 cgcttcatta taggttttat catcaaaccc gattgagaaa gaatgatctg gtttcaatac
    32701 agaggttaca taactggaat caaccccgct ggataagaaa gaaccaactt ctacatcact
    32761 gaccgtatgt gcttcaatag aagcttgaac ggtttcgtcg attttttcga tccattcttg
    32821 tcgggaatgc gtttcttttg tttcaaaatc tgcatcccaa tattgctgaa tgtctagttt
    32881 tccatcttta tacgtataat aatggccttc aggcaaacga tagacatctt tgaagaatgt
    32941 ttcgccgttt aatggagaat attggaaagt catataaggt ttcaacgctt ctttgttcaa
    33001 ttctttgtta aaatcaggat gaggcagaaa acttttgatt tctgaaccaa acataaatgt
    33061 tccgttcatt tctgcataat aatatggttt gatcccaaag tggtctcttg caccgaataa
    33121 ctcattcttt tcattatccc aaatagcaaa agcaaacatt ccgcggatct tttgcaataa
    33181 ttctgttccc cattcttcgt aaccatgcaa aaggacttct gtatctgcat gtgtttgaaa
    33241 aacgtggcca gctgcaatca attcttctct caaaggctgg taattataga tttcgccatt
    33301 aaaaataatg atctttgtcc gatcttcatt gtaaataggc tgagtaccgc cctctaagtc
    33361 aataatactt aatcttctga atcctaaagc aacatgttta tcgatatatt ctcctgaact
    33421 attaggtcca cggtgaacga ttcgatccat cattgtatta ataattgttt ttttgttgtc
    33481 cttgtcgtta acaaaaccta caataccgca catttaactt tcacatccta atataaaatt
    33541 actgtttatt tcattcatat gcatcttatc atagaataaa tagcggacga atattttctc
    33601 tcttacaatt tcgtcacgtg aaaacaaaag atgaaagttt ctgtttttac agaaaataaa
    33661 gtcccaatat ctaatttacc atgttttctt ttttgaggct accttattct cttcgtgtta
    33721 aaaaaatatt agtggatgct atttccaaaa aaaacagcaa aaaaagacac gacctaaaga
    33781 tcgtgtctaa ttatgactgt atcagaaaat atgataccaa acggatctat tcattaatta
    33841 attgacatag tttatctgtt gctcttttat gactggataa ctgaccaatt catgacgatc
    33901 caagtcttct ttatttatat ttacagcagt aatttgactg tcttcatacg tacgataata
    33961 ataaatccct ttatcaacgt tacagcaaga agaataaatc gtatattcgt attttccttc
    34021 tccagtatcg catactcctt tttgttgctc gactgagcct agtatatgaa aaaattgact
    34081 gatactttca gattctgaat cacctgatgc agcattcatt ttagtaaatg tggcttttac
    34141 aaatcgagaa acagaagaga gatctccagg taagcccaat cctcccatcc cacgactata
    34201 aacattcaag ctgatctggt ttgaaaaatg attttctggt gttccatttg atagcgagcg
    34261 ataattgttc agattaaata attgataatc aaatggcgga ttatttgtca acacaccgac
    34321 aggattatca tataaatgaa gtccatcttt catactttca attactatcg atttttcttt
    34381 atcagctaat aaccagtgta aaggtgacaa gggtagtttt tcactaaaat cgatattgac
    34441 tagattgata gtagccaata attctttagc ttcgtctatg gttgagcatt gccctagtat
    34501 ccatggaata aactcaaatg gtgcaacgtt atcttttcct tcttgtaatt ctttataatc
    34561 agcattgcca ggaaaattca gtcctgccat actcaaccct ttttcatttg tagcctcata
    34621 atagagtgga tagttggata ccccagctgc tattcctatc attgcataat gtttatctaa
    34681 attttttacc tttctaaaat cgaaacgata atttcttgga gtaatagtga ctacttcatt
    34741 ataagacatc tcataatcaa aattccttcc aaaataatgg tctttcgttg cataagtgat
    34801 tgcggtacac atcgttttcc ctcctaaaac aaatcgtttt tacactttta tcttacatct
    34861 agtatttttt tttatcaaaa aaaaggccaa agaagattga tttactagca tttataaaaa
    34921 atagtactca gggggaatca aaaaatattg taggttaaaa gtacaacaat aaggggctgt
    34981 gacaaaagtc ttgtcacagc cccttattcc gaacaaacgg tgttcaaaag cttgcatctg
    35041 cggtaataag cccaaccaaa cgaaaaaata ggaaaagcta tttttgtttg gccgttctta
    35101 ttacttgatg caggacagct tcttcacaac ctctaatcgt tgatataagg aaggtacagg
    35161 atttgaacct gcgcgccggt attagccggt tcgccggatt tcgagtccgg tgcattacca
    35221 ctctgccaac cttccgtaag tactattttt aaactttttt cacgaaatgt caagcctatt
    35281 tctaatagat aaataacaga aatcgctttt tttagcaaaa aagtccattg acagtttgca
    35341 gccaacggac ttaccgatca aattcatact gatagaacac tatctgatta tttcaggatt
    35401 atctaatgct aaaatcgagt caattgtccg tttgatcagt gcaatgattt gttcttctgt
    35461 tggatgcatg atgtttgatg acatcaatga cgctaagcca gtcactgcta cccatgtacc
    35521 agtatgcaga gcatcgacat attcatccga taattgattg tatctttcat cttctttaac
    35581 tatttcacgg aaataattgt atgaaaaatc atgcattttc tttccgtccc catgttcatc
    35641 aatgtacaat gccatgtaaa gactatgatg cttttttgca aagtagatat agttcaaacc
    35701 gagatcaatg attttatcac cagtacgttc aatagggaaa acatcttctt ttagttgttc
    35761 aaagatagaa tccagcaacg tattttttaa atcttccatg tttttgaatt ccagataaat
    35821 cggctgtgtc gaaatcccca tctttttagc aatatttctc gcagtaaatc ctttaaaccc
    35881 ttcctttgca acaacatcat aagcagcttt tagaatttga tccttggtat agacttttct
    35941 tctcattaaa actcccccac ttctttactg cagtatatgt tgttttattg taccctattt
    36001 ttagcatttt ttgcaaacat tttactcaaa acgttatatt ttaaattttt ttcagtcttc
    36061 tttaactttt ttttgcaaaa ttaaaaaaat ttggttaaat tcaatgggtt cccttacatt
    36121 tttagttttg acctaaatga tagttgtcca aatctttcat ttcaagttat agttaaagaa
    36181 aatgaaaaag aaggtgtgta atggaaaacg aaactgacgt tatttatatt catcctcaaa
    36241 aacgaatcgt ttcacagaaa agaaagtatt tttatcttgg ttttacaggt gttttttttc
    36301 tttttattgg cttgctttct aatactccta ctgataattg gagtggattg ctaactattc
    36361 ttacctcgcc aagcaatctt ttgacagatt attttgcttt aggtgttttc ggaagcgcat
    36421 tcataaatgt agggattttg accttacttt ctgtgctttt agcttatcgg cacaaggtga
    36481 ttttaaacgg tcccttattt gcttcgattc taacagttac cggtttctct ttttttggca
    36541 aaaaccttta taattctatc tcaatcattt taggtgtgta tctatacgct gtatttgtca
    36601 ataaaccttt ttctcagtac atcatgatcg gactgtttgg tagcgtatta tcaccggtcg
    36661 ttagttatat cacttttggt atgcgttttc ctttactgat aggtatttta ttggggaatt
    36721 tagctggcat agcaattggt atacttcttc cgcccttagc tgctcaaact ttagttttcc
    36781 atcgtggatt cacactttat aatattggat tcacctctgg gttgattgcg atgactttta
    36841 ctgctgtttt gaggttattc agctattcaa tcgtcgaaaa tacattggtt ttcaatgaat
    36901 atcactttcc tctcatttgg attatttttg gctttttttc gctgacagtc ggaattggtt
    36961 tctattataa ttcctttcgg ctttcgggga ttcgagaaat tttcgattca tctggaaaat
    37021 tgacaactga tttcattgct aattctggta ttggtgctac tttgatcaac atgggcttag
    37081 ttggtctgat gctgtcttct tatgttttgc tggttggcgg tcagttaaat ggacctgtca
    37141 tcggtgctat tttatctgcg gtgggtttca gtgcttttgg ttgtcattta aaaaacagtt
    37201 ttccgatact agtcggaata tttattgctt ctttgtttgg aacttttcat gagatcacca
    37261 gtactggcat gcttgttgca gctgtgtttg ggacaggtct tgcgcccata agcggttttt
    37321 acggcagttt ttacggtgta atagctggtg tgctacatat tgctcttgtc cataacgtca
    37381 gcactttgca cggaggacta aatctatata acagtggttt ttcaactggt ttcgttgcag
    37441 gtatccttgt accgatacta gataacttta ccgctgtcag aaaggaaaaa aagacacttg
    37501 gaaaaagaat tatcaaaaag aatcatcgat gagcttctta cttatttata ttcccatgaa
    37561 ataactgaaa tacgtattgg agtagaattt gctgaggaag ggcttttcat agagatacag
    37621 ggtacggcag aaactcagcc agaagattta gatgtacttg ctgatttgct gaacaatcct
    37681 cgggacccta gtatggaaat ctactatcaa gaactattag gtaatccccg acatgatcgg
    37741 gaagattatc atctgttagg tagtttgatt gatgacgtgg aaatcatgta tgcccagccg
    37801 attatcaata ttaagatctt tcgacgtgct tctcattcct attattaacc ctattttcta
    37861 tttataaaag actgaatcaa aaaagcagct cctgaaagtt cgattaaaat ctactttcag
    37921 gagctgttca agcttgacat tattgatttt ccaatccttc atacacttca tctgttacag
    37981 gttctaaaaa ctctgaatta ccagcagtga tcgccaaatg ttcaaaccaa ctgtcttttg
    38041 tcgcaccatg ccaatgttta atccctttgt gcgttacgac gacatctcca gccattaatc
    38101 gtcttgctgg ctttccttct tcctgatacc agccttctcc tccggtcaca agtaagattt
    38161 gatagccgtc tgtatggaca tgccagtgat tgcgacaacc tggttcaaaa gttacattac
    38221 ctacaccaac agaaacttcg ggatcattca ctaacatatt cagatagctt tgcccggtaa
    38281 atacttgagc gtaagcttcg tttttttcac ctgctggaaa aatcacgcca ttgtgtattt
    38341 cttcgtgttt tgtcatcatt tacgcctctt tcttatcttc gctattttct tctaaataga
    38401 tttcttttgc taaactaaaa gctgaccaag cttttggcca ttctacataa aaagctaaat
    38461 gggtaatgat ttctgctatt tcttcactag taatgccatt ttgcttgcca atcttcagat
    38521 gagctggcaa ttgttcaaaa tttcctcctg aaattaatgc agatatggtg atgatactac
    38581 gctgatgagc agataattct tctctggacc atacttgtcc aaataaaaca tcatcattta
    38641 attctgcaaa ttttggtgca aaatctccta attgatctcg tcctgcggtt tgtttttctg
    38701 ccatttctct tattctccct tcacgactgt taggtgattc aactatggtt ctaaggcaac
    38761 tttattttct attgtttctt cgcatttcat tttaaatgta gtctttcagc ctgtccaatg
    38821 cttagtttat tcagtcgata tgctttttaa gcatagttgc taacgctttt tttattttaa
    38881 aaaaaacaag ttattaaata agtctttagt tggatgtttt tttgagatca atcttttata
    38941 cttatgatga acttacaaaa ttgtttgttt tctgtcatat caaaaaatgg cgcagttagt
    39001 tgcttttcta gtaaagtata gttaacaaag aacctctaag gagttgatga ataatgaata
    39061 ttgctgcctc tcgaaaactg ataaatttca tatccattat cggattaggc ctatcaatcg
    39121 cgctgacgat ttattttatt aatttaggtg ttttcaaaga tttgaatgcg ttaagaggat
    39181 tagtaggtga ttccatcatt ttaggaccga ttatttttgt cttcatccaa atcctgcaag
    39241 tagttattcc aatcattcct ggagggatca gtactgcagc tggtgtcctg atatttggcc
    39301 cttatgctgg atttatctat aactatattg gaatctgcat tggttcgatt atcatttttc
    39361 tgctagggcg tcgttatgga aagccattca tactttccat gattagcgat aagacatata
    39421 acaaatatat tggctggctg gataatcaaa accgttttga gaagctgttt gctctagcta
    39481 tttttcttcc agttgctcct gacgatgcgt tatgtttgat ggctggattg acaaacatgt
    39541 cagtgaaacg atatacacta atcattttga ttgctaaacc actgtcgatt ttcttatata
    39601 gtatggcctt gatttatgga ggtcaatatc taagtggtct tcttggataa tatttatttt
    39661 gtaaaattag tcattgattg acaatgtt
//
LOCUS       DACDYJ010000021        34984 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDYJ010000021 DACDYJ010000000
VERSION     DACDYJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.021"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            299..1960
                     /locus_tag="IWM51_001094"
     CDS             299..1960
                     /locus_tag="IWM51_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9970528.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="IWM51_001095"
     CDS             complement(2030..2377)
                     /locus_tag="IWM51_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP9970529.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="IWM51_001096"
     CDS             2461..3402
                     /locus_tag="IWM51_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9970530.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="IWM51_001097"
     CDS             3403..4446
                     /locus_tag="IWM51_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9970531.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="IWM51_001098"
     CDS             4452..5522
                     /locus_tag="IWM51_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9970532.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="IWM51_001099"
     CDS             5512..6465
                     /locus_tag="IWM51_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9970533.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="IWM51_001100"
     CDS             6477..7487
                     /locus_tag="IWM51_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9970534.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="IWM51_001101"
     CDS             complement(7772..8593)
                     /locus_tag="IWM51_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9970535.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="IWM51_001102"
     CDS             complement(8690..8968)
                     /locus_tag="IWM51_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970536.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="IWM51_001103"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="IWM51_001103"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP9970537.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="IWM51_001104"
     CDS             complement(11724..12311)
                     /locus_tag="IWM51_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970538.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="IWM51_001105"
     CDS             12486..13808
                     /locus_tag="IWM51_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9970539.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="IWM51_001106"
     CDS             13963..14739
                     /locus_tag="IWM51_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9970540.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="IWM51_001107"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="IWM51_001107"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP9970541.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="IWM51_001108"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="IWM51_001108"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP9970542.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="IWM51_001109"
     CDS             16629..17258
                     /locus_tag="IWM51_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970543.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IWM51_001110"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IWM51_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP9970544.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="IWM51_001111"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="IWM51_001111"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP9970545.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="IWM51_001112"
     CDS             19445..19981
                     /locus_tag="IWM51_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9970546.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="IWM51_001113"
     CDS             complement(20021..21784)
                     /locus_tag="IWM51_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9970547.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="IWM51_001114"
     CDS             complement(21787..23502)
                     /locus_tag="IWM51_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9970548.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24281..24505)
                     /locus_tag="IWM51_001115"
     CDS             complement(24281..24505)
                     /locus_tag="IWM51_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970549.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24705..27473
                     /locus_tag="IWM51_001116"
     CDS             24705..27473
                     /locus_tag="IWM51_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9970550.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27554..27973
                     /locus_tag="IWM51_001117"
     CDS             27554..27973
                     /locus_tag="IWM51_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP9970551.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27985..28455
                     /locus_tag="IWM51_001118"
     CDS             27985..28455
                     /locus_tag="IWM51_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9970552.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28472..29251
                     /locus_tag="IWM51_001119"
     CDS             28472..29251
                     /locus_tag="IWM51_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9970553.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29238..30059
                     /locus_tag="IWM51_001120"
     CDS             29238..30059
                     /locus_tag="IWM51_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9970554.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30076..31125
                     /locus_tag="IWM51_001121"
     CDS             30076..31125
                     /locus_tag="IWM51_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9970555.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31138..32142
                     /locus_tag="IWM51_001122"
     CDS             31138..32142
                     /locus_tag="IWM51_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9970556.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32775..33794
                     /locus_tag="IWM51_001123"
     CDS             32775..33794
                     /locus_tag="IWM51_001123"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9970557.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            33837..34718
                     /gene="galU"
                     /locus_tag="IWM51_001124"
     CDS             33837..34718
                     /gene="galU"
                     /locus_tag="IWM51_001124"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9970558.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT        11470 a         6174 c         7173 g        10167 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacgtctat tttacttaat ttttctataa atgaattctg cataaaaaat atatcaagta
    23761 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atcttttttg
    23821 ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc aaagaaggag
    23881 tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga ggaataaata
    23941 atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa cgaagacaac
    24001 ctcccgacat caagtcaaaa gctacttgag attttgatct gaaaataaaa aaagaaaaca
    24061 aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg
    24121 gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc tgaaagcatc
    24181 ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat aaggatttgt
    24241 aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta tggaaggatt
    24301 tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct atatattgcg
    24361 tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa ggcaaaaata
    24421 caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa gtatgtttcc
    24481 tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct ttcatatgtt
    24541 gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa attataaggg
    24601 ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa agattttatt
    24661 gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg aagaaggaaa
    24721 ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg aacgaggtct
    24781 ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc agtcattatt
    24841 tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca gtgatttatt
    24901 tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt tatttttcta
    24961 tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta ttctctttta
    25021 tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca gctctttact
    25081 atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt aaaagttatt
    25141 tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat tctgcaccat
    25201 tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact agcaatttgt
    25261 ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg gcttttgaag
    25321 cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca gaaggtcagg
    25381 aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa cctaatttgt
    25441 cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact agtcatttct
    25501 taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt aatcagcgta
    25561 gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa agaaaaatgg
    25621 ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag aaattatccg
    25681 gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca tatgctgaac
    25741 agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa ctacttgcgc
    25801 agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac cagaccttag
    25861 agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt tttgagcaaa
    25921 tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagga aaattaaagc
    25981 aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa gaaaaacacg
    26041 agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg gaaactgcct
    26101 atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc tatcagcgaa
    26161 gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa gaattagaac
    26221 aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt ctggaattaa
    26281 gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg acgatctatc
    26341 taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt gctgctcacg
    26401 gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa gaggatctat
    26461 ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca gaaatccttg
    26521 attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac atgggatctc
    26581 tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg attttgaata
    26641 atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat gtatcattag
    26701 aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg ctctatccag
    26761 aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt acggcggtcc
    26821 acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa atcatcccat
    26881 atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc atatacgaag
    26941 tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat ctttcattag
    27001 aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac actactatga
    27061 atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca ttggaaaaag
    27121 tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc gatcatttta
    27181 tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa ttagcgttgt
    27241 atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc gaaacctatc
    27301 aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt aaatctgcgt
    27361 ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt gcctatatct
    27421 atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt tgaacataag
    27481 caagttgttg ccactaattt tggcatgcaa cttgcttttt tagtatgtgt aagtgaaaag
    27541 aggagatgaa aaagtggata gagaaattat cttagcttcc catggaaaat ttgcttcagg
    27601 gatattagat tcattagagc tgatctatgg gaaaaatcat tctattactg tattcgattg
    27661 ctatactgat gagaattttg atttagcgga aacagtaagg cagcttttta tgaggtacaa
    27721 agataaagaa atcattgttc tgacggatct ttttggcgga agtgtcaaca acgaattctt
    27781 acaatatatc aatcgggatc atgtctatct cgttgccgga ttgaatttgc cactattgat
    27841 tgagctagta tctcggttgg acgctgaagt agagacaact gttttgatcc agcaggtgct
    27901 aactgattca aaaaaaatga tccagttttg taacgagcgg ctcaatcaga aaatagaaga
    27961 agacgaattt taggaggaag aaaaatgatt gtattgacaa gagtcgacca tcggttatta
    28021 cacggacaag tagctttttc atggacacaa acaataggcg cagactgtat tttgattgct
    28081 aatgatgatg tacctacgaa tgagatacga aagacaacga tcaaattagc caagccacaa
    28141 ggagtcaaac tagtgatcaa atcaattgat gactcgattg ccgcgttgaa aagcggggtg
    28201 acagataaat ataagttatt catcgtagtg gaatcgatcg aggatgcata taagctggca
    28261 gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga tcaaggcaaa acctggtact
    28321 cgcagcatcg gaaaagcggt aaatatttta ccagaagagg aggaacgctt gaaacgatta
    28381 attgacaaac aaatagaagt agaaatccgt caagtaccag cagataaaaa agtggatgta
    28441 agagaagttt tgtaagaaag gaagaacctc aatgattgta caagcagtct tattaggaat
    28501 tgttgcattt gtcgcacaat cagaatatgc actagggaca tcgttgcttt ctcgtccgat
    28561 tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta aaaactggga tcattatggg
    28621 cgcaacttta gagttagcat ttatcggatc gttctcagta ggtgcttcga ttccacctga
    28681 tgttgtgacc ggtgggattt tagggacggc ttttgctatt acagctggag ccggtacaga
    28741 aaccgcgctt ttactaggtt tgccgattgc tacgttgaca ttgatattga aaaatattta
    28801 cttagggctt ttgattccaa ttatgaacca taaagcagac aattatgcag aagaaggaaa
    28861 ttataaagga gtcgagcata tgcacttgct tgccggtatc ggtctttcct tgatgcttgg
    28921 tgttgtggta atggtttctt atatggtggg aagcaatacg atcggaaatc tattaaatat
    28981 gattcctgac ttcgttcaaa aggggttgtc agtagctaca gggttgattc cagctcttgg
    29041 ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt gcaccatatt ttttcttagg
    29101 ttttgctata gcttcttact tagagattcc tgtgaccgga gtagctattt ttggtgcgat
    29161 tttagcagtt gtggtagtca atattatgaa tgtccgtcaa ctaccacttc aaacaactgc
    29221 aggggaggta gaagaagatg aagaattctg agatcaaaaa cagcaaaata acgaaaaaag
    29281 agctaaatca cgtattttgg cgttcttttc aaatggagtt ttcttggaat tatgaacgtc
    29341 agatgaatat ggcttatgtt tatgcaatga ttccgatttt gaaaaagtta tatcagtcga
    29401 aagaagaaat ggcagcagct ttgaaacggc atctagagtt ttttaacacg accccacata
    29461 tcgttacact gattttagga atcacggctg caatggagga agaaaataag gatgatcctg
    29521 aatttgatgt gactgcgatc gatagtatca aaacatcttt gatgggacct cttgcagggt
    29581 taggagattc tttcttttgg ggaactttgc gattgattgc cacaggtgtg ggtacctctt
    29641 tagccatgca aggaaatatt ttaggcccta tcttgttcat tttgattttc aatattccgc
    29701 acattctgtt tcgctatcta gcaacaggtt ggggatatcg cttaggaacg ggttttttga
    29761 aaaagatcca ggcaaacggt atgatggaaa gtttgacatt aggcgcgtct attattggac
    29821 tgatggtcgt tggagcaatg actgccacga tgatcaccat caatattcct ttaaaaatcg
    29881 gttcaggaga aaatgctgtg actgttcaaa gtatttttga tgatatcgtt ccaaatatct
    29941 tatgtcttgg agcatttggc atagtgtttt atctattgaa gaaagaagtg aaaccattaa
    30001 cgatcttgat cggactagct ttctttggaa tttttggttc gtggataggg attttttaaa
    30061 aaaggagcag agaaaatgtt tacgatgcaa gattatatct atgaagaaaa ggaagtacta
    30121 agtacgatct tgaaaaagaa tgatttttcg acaagagaac atatgaaaaa aacagtgaat
    30181 ttactgatct tagcaacggg ttcatcttat aacgcttgtt tagcagcgaa gccagcattg
    30241 gaatcttatg gtgatcttac tgtcgatatt caagaacctt tttatttttt caattatgga
    30301 aaactttctc cttcaatcga tactgtgatt gctgtgtcac aaagcgggaa aagtgcttct
    30361 actgttgaag cagtaaaaat gatacaaaaa caagggttac ctgttgttgc tatcacaaat
    30421 gacgtgcaaa gtccacttgc tttagaagct gcacaaatta ttgatttagg tgccggggta
    30481 gaatcagtag gcttcgttac aaaaggctat tctgcgactg ttttacagtt actactctta
    30541 gggataggaa ttggaataag taaaaataaa atcagcaaaa aaatagaaca agactacatg
    30601 caacagttgc gcaaaataat caaccattta ccagcgatta ttcaaaaaac ggaagaattt
    30661 tttgatggat atcaaagttt atttcgttta gcacagcgtt tcgtcaccat tggatatggt
    30721 ccaaactggg gaacagctaa agaagcagag acgaaattga ctgaaacaat ccgcgttccg
    30781 tcacaaggat ttgagttaga agcttatatg cacggcccat acctggaggc agatgcttcg
    30841 catttgctat tctttattga aggggaaagt gtaaataagg aacgttcgca aaagttgcaa
    30901 agatatatga gtcggtacgt tggagagact ctcaccatta ccacaaaaaa agcaagaaac
    30961 gagaaaacac ttggattagc aatagaatgt gatgaatatc tgtctgtatt agcgcttgtc
    31021 gttccgtttc aactattcgc ttataaaacc gcagtagcta aaggtattga cttgaacaaa
    31081 aagatttttg aagactttga tacagtcttg aaaagtaaat tataagaggt ggaaatcatg
    31141 ctgaaattca atgaagaaga acaaatcaaa gcaataaaag gcgcactagc tttacgtccg
    31201 caagtagaag aaatcatcga taaactttat gcggaaaaat ttgacgctgt ctattatcta
    31261 ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca cttatatgaa tgggaaaagc
    31321 aacttgcctg tctttgtaca gcatgctgca gaatattaca ctactggaaa caaaagatta
    31381 acaaaagatt ctatcgttat tttgtcctct gttaccggta caactcaaga agtagtgaaa
    31441 gcagtagaac aaatcaagaa agtaggagcg acactgattg gatttatcga taaagcgaac
    31501 agtaagttgt ctcagctatg cgattttgtg gtcacttatc cagctcctgg aacagaacaa
    31561 atcaaatttt ttatggcagc agatcgattg atgtatttga atggagaatt cgaagcttat
    31621 tcagaatact atgaacagct cgaacgttat ttaccaacag ggttagtgga agcagagaaa
    31681 aaagcagatg catttggctt atcatttgca gaaaaacatc gacgtgatag tatgcattat
    31741 tttattggag cagggaatca atggggagct gtttattcat atgccatgtg ttactgggaa
    31801 gaacaaagct ggcttccatc gaaatctatc catgctgccg agtttcttca cgggacgtta
    31861 gagatcgtag aagaaacaac cccagtcaca ttatttttag gcgaagatga acagagagtg
    31921 ttatctgaac gtgtggctaa acttttgcca aaaatttgca gcaattatac attgatagac
    31981 acaaaagact atccagtaga aggtatcagt gaaaaatatc gcggacgtgt tttgtctttc
    32041 ttattgatgc atgtagtgac acaacgcatc gatgctcatg tggagcagtt gaactgccat
    32101 ccattagaga tacgccgcta ctatcgtcag tttgattatt aggatagact ggataaaaag
    32161 aatataaaaa ataaagagag cagcgataac tgctcccttt atttttttaa aatattggca
    32221 tcaattacgt tataaaacac attttttgca ttatctacag ttcaacttat aatctgttta
    32281 atgggacatg tgggtgccaa ccattttttg ttatgcaata gtactattta gttgttgcat
    32341 ggatccgtag taaaagtcca agtacaacga gttgcgccag tttacatagc gttttgtatc
    32401 cagaatgttg actaaaacaa aagttcctat gtcaaccgtc aacagagaca atctgtccgt
    32461 tgcaggacct gacagattta gaaatgttca atctgtctct tatgtatttt aaatcaatac
    32521 tcactaggag aagtttccta acctcctaat agtaatgctg gtagtaacat tgtcaaaact
    32581 gcttcctctt aggaacaagc aaagcataac gagtgtaaaa ataaaatgta gactattgta
    32641 acatatcatt gattatttca agtaaaaacg ataaaagaag taaacaaaca cttaaaaaca
    32701 atggaaatta gtcccaactt taagaaatgt atactataat ggaaattatc aattatttat
    32761 ttggaggaat atccatgaaa caaaaaatcg cagttcttgg tcctggatct tggggcactg
    32821 ctttagcaca aacattggca gaaaacgggc atgatgtccg tatttgggga aatgtacctg
    32881 aacagattga tgaaatcaat aagtatcata caaatcagca ttttttacct gatttgacga
    32941 tcccagaatc gattatcgga tataaagaat tggcagaagc agtggataac gcagatgcta
    33001 ttttatttgt tgttccaaca aaagcgattc gttcagtcgc acaggaactt attggtaaaa
    33061 tgaatactaa gccaatcatt atacatgcaa gtaaaggact agaacaagat acgcataaac
    33121 gaatctcaga agttttggct gaagaaattc cagaagaaaa aagacaggca attgttgtat
    33181 tatctggtcc tagccatgct gaggaagtgg cagtacatga tatcacaaca attacagctg
    33241 caagtattga tcaaaaagca gcagcctatg tacaaaaact gttcatgaat gattatttca
    33301 ggatttatac aaataatgat gtgattggcg tagaaactgg tgcagcactg aaaaatatca
    33361 ttgcaattgg tgccggcgct atccatggat taggattcgg agatgatgca aaagcagcaa
    33421 tcatgactcg aggattggca gaaatcagcc gtcttggtgt cgcgatgggt gccaatcctc
    33481 tgacatttat cggtttgagt ggtgtggggg acttgatcgt tacttgtaca agtgttcatt
    33541 ctcgcaattg gcgtgccggt aacttgttag gacagggaca taagctggaa gaagttctag
    33601 aaaacatggg aatggtcgtt gagggtgtgg caacgacaaa agcagcagta gagctagctc
    33661 aacagctagg tgttgagatg ccaattactc aaacaatcta taatgttttg tataatggag
    33721 aagatatcaa aaaagcagca aaagaaatca tgttacgtga cggaaaaatg gagaatgaat
    33781 tttctcttcg ttaaatagat gatcaatagt taatcgtcgt ttaggaggaa cagcaagtga
    33841 aggtcaaaaa agcagtcatt cctgccgctg ggctaggaac acgttttttg ccggcaacaa
    33901 aagccatggc aaaagaaatg cttccaatcg tggataaacc aacgatccag tttattgtgg
    33961 aagaagcatt ggattcggga atcgaagaca tcttgatcgt aacgggaaaa gaaaaaagac
    34021 ccattgaaga tcactttgat gcaaatatcg aattggaaaa caatttgcgg gaaaaggaaa
    34081 aaacagaatt actcgcttta gttgaagaaa cgactcaagt caatcttcac ttcatccgtc
    34141 aatctcatcc aagaggactg ggggatgccg tgcttcaagc aaaagcattt ataggaaatg
    34201 aaccgtttgt cgtcatgctg ggggatgatt tgatgaaaga tgatatccca ttgactcaac
    34261 aattgatgat ggattatgaa gaaacacaag cttccgctgt tgccgtcatg cgtgtaccag
    34321 aaaatgaaac atctaaatac ggaattattg atcctaaaac agatagcgaa aagggtcgtt
    34381 gcagagtaaa agggtttgta gaaaaacctg aaatgggaaa agcaccaagt aatttagcca
    34441 ttatcggacg ttatctactg actcctaaaa tatttgagat attagaaact caagaaccag
    34501 gagcaggaaa cgagatacag ctaacggatg cacttcagac tcttaaccag acagaggcgg
    34561 tctatgcgag agagtttaaa ggaaaaagat acgacgtagg cgataagtta ggatatatga
    34621 agacaaatat tgaatatgga cttcaacatt cggaaattgg gacttcatta agtgcatata
    34681 ttatccagtt gagtaaaaag ctaagaacag aaagttaact ggaaataagt ggcctgctga
    34741 gaatgaaagg ctatttgtta ttaaagcggc agcttgtgcg tcatttgcgc aactgtcgtt
    34801 ttagtttttt gactctagat cagttgagtc gtggaaaacg aaagatagaa aaagactttc
    34861 gtgaatagat tcagaaaata ttatgctaat ggtttaattt ttttttaagt tgtataaaag
    34921 aaaatgatta cataagagaa atgaaagcca agccagaaga ttcatagtag aataaaaagg
    34981 cgaa
//
LOCUS       DACDYJ010000022        34710 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDYJ010000022 DACDYJ010000000
VERSION     DACDYJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.022"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(362..2254)
                     /locus_tag="IWM51_001125"
     CDS             complement(362..2254)
                     /locus_tag="IWM51_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9970559.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2251..3978)
                     /locus_tag="IWM51_001126"
     CDS             complement(2251..3978)
                     /locus_tag="IWM51_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9970560.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3994..4644)
                     /locus_tag="IWM51_001127"
     CDS             complement(3994..4644)
                     /locus_tag="IWM51_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9970561.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4848..5144
                     /locus_tag="IWM51_001128"
     CDS             4848..5144
                     /locus_tag="IWM51_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9970562.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5336..5956
                     /locus_tag="IWM51_001129"
     CDS             5336..5956
                     /locus_tag="IWM51_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP9970563.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6048..7214)
                     /locus_tag="IWM51_001130"
     CDS             complement(6048..7214)
                     /locus_tag="IWM51_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9970564.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7226..7579)
                     /locus_tag="IWM51_001131"
     CDS             complement(7226..7579)
                     /locus_tag="IWM51_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9970565.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7707..8729
                     /locus_tag="IWM51_001132"
     CDS             7707..8729
                     /locus_tag="IWM51_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP9970566.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9168..9602)
                     /locus_tag="IWM51_001133"
     CDS             complement(9168..9602)
                     /locus_tag="IWM51_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970567.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9712..11571)
                     /locus_tag="IWM51_001134"
     CDS             complement(9712..11571)
                     /locus_tag="IWM51_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9970568.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11689..12848)
                     /locus_tag="IWM51_001135"
                     /pseudo
     CDS             complement(11689..12848)
                     /locus_tag="IWM51_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(13155..14579)
                     /gene="araA"
                     /locus_tag="IWM51_001136"
     CDS             complement(13155..14579)
                     /gene="araA"
                     /locus_tag="IWM51_001136"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP9970569.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14595..15293)
                     /locus_tag="IWM51_001137"
     CDS             complement(14595..15293)
                     /locus_tag="IWM51_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9970570.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15250..16911)
                     /locus_tag="IWM51_001138"
     CDS             complement(15250..16911)
                     /locus_tag="IWM51_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP9970571.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17129..18529
                     /locus_tag="IWM51_001139"
     CDS             17129..18529
                     /locus_tag="IWM51_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP9970572.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18781..19860)
                     /locus_tag="IWM51_001140"
     CDS             complement(18781..19860)
                     /locus_tag="IWM51_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9970573.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19946..20905)
                     /locus_tag="IWM51_001141"
     CDS             complement(19946..20905)
                     /locus_tag="IWM51_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP9970574.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20917..22440)
                     /locus_tag="IWM51_001142"
     CDS             complement(20917..22440)
                     /locus_tag="IWM51_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9970575.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22450..23013)
                     /locus_tag="IWM51_001143"
     CDS             complement(22450..23013)
                     /locus_tag="IWM51_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9970576.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(23019..23882)
                     /locus_tag="IWM51_001144"
     CDS             complement(23019..23882)
                     /locus_tag="IWM51_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9970577.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23896..24792)
                     /locus_tag="IWM51_001145"
     CDS             complement(23896..24792)
                     /locus_tag="IWM51_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9970578.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24967..26238)
                     /locus_tag="IWM51_001146"
     CDS             complement(24967..26238)
                     /locus_tag="IWM51_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9970579.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26228..27745)
                     /locus_tag="IWM51_001147"
     CDS             complement(26228..27745)
                     /locus_tag="IWM51_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9970580.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27943..28833
                     /locus_tag="IWM51_001148"
     CDS             27943..28833
                     /locus_tag="IWM51_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP9970581.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28906..29730)
                     /gene="nadE"
                     /locus_tag="IWM51_001149"
     CDS             complement(28906..29730)
                     /gene="nadE"
                     /locus_tag="IWM51_001149"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP9970582.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29742..31214)
                     /locus_tag="IWM51_001150"
     CDS             complement(29742..31214)
                     /locus_tag="IWM51_001150"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP9970583.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31851..32213)
                     /locus_tag="IWM51_001151"
     CDS             complement(31851..32213)
                     /locus_tag="IWM51_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9970584.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32436..33188
                     /locus_tag="IWM51_001152"
     CDS             32436..33188
                     /locus_tag="IWM51_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9970585.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33328..34251)
                     /locus_tag="IWM51_001153"
     CDS             complement(33328..34251)
                     /locus_tag="IWM51_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9970586.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34266..34538)
                     /locus_tag="IWM51_001154"
     CDS             complement(34266..34538)
                     /locus_tag="IWM51_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970587.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10245 a         7222 c         5989 g        11254 t
ORIGIN      
        1 gcataaattt ggcttaatac cgagaaactg gcttttgaac accgtttacc aagaggttgt
       61 gagcctgccg tttaactccg agtcacaagg agcaatttca aaaaacagct tctcaggttt
      121 gttgactatt gtgacttgtg accgaggagt tggcaggaga acaccgttta aggaagaggt
      181 tgtaaaatca gcggtttgac ctgagtatta gagatacaaa acggaaaaat agcttctcat
      241 attttgccgt tttttgagct aataccgaag gacagctgat tgaacaccgt ttattcggaa
      301 aaagggactg tgacacgact tttgtcacag cccctaaaaa aaataataaa tttgtactat
      361 tctatgaagc ttcttcttca gaaaactgac tgttataaag atctgcatag aatccgtctt
      421 tttccatcaa tgtatcgtgg tttcctgttt ctacgatcga accatgattc atcacaataa
      481 tgttgtctgc atcgcgtatt gtagacaagc ggtgagcaac aacgaaactt gtccggtttt
      541 ccaataggcg gttcatcgct ttttgaatca gtatttctgt tcgtgtatct acactagatg
      601 ttgcttcatc aaggatcagg acatctggat ttgctaagaa tgcccgcgca attgtgatta
      661 gctgacgctg cccttgcgaa atattgcttg catcctcgtt taatacagtg ttatatcctt
      721 ctggcaattt acgaacaaaa tcgtctacgt gagcagcttt agcagcctca atgatttgtt
      781 cgtctgttgc atcgtcgttc ccatatttga tattgtcata gatacttcct gtaaacagcc
      841 aagtatcttg cagtaccatt gaaaactgtg cgcgcaattc atcacgtgaa agatcacggg
      901 tatctacacc atcgtaacga atactacctc cagaaacatc gtagaaccgt tcaagaaggt
      961 tgatcagtgt cgtcttccct gcaccagtcg gacctacaat agcgaccatc tcaccaggtt
     1021 tgacatccaa gttaaaatca gtcattagca agttatctgg tgtgtagccg aattgaacat
     1081 tttcaaaacg aactttgtat ggtgtatctt tttcttctgg aaggccagat ttttcattgg
     1141 acatctcttc ttcatccaac acttcaaata cccgttcggc tgaagcaact gttgattgga
     1201 tagtgttcat caaacttgca agttgagtga ttggttgaga gaactgtgtt gtatattgca
     1261 agaacgcttg aacatcccct aatgtcatgt tcccatttgc aacttttact ccgcctagta
     1321 cagcgacgaa cacatagcct agatttttaa cgaaattcat caacggcatg atgattgccg
     1381 aaataaattg agctttccaa ccagcggcat acagattctg attttctttt tcaaatactt
     1441 cctggtcttt ttcagtatga ttgaatgttt taacaatggt atgaccacca tatgtttctt
     1501 ctacttggtt attcaataaa cctaaacttt tttgctgatc tgcgaaaaac acttgtgatt
     1561 tcggcgctac gatcatcacg atgatcaagc ttaaagggat catcaataac gcgactaacg
     1621 tcaattgcca gctgattgaa agcatcatca cgatgacccc aatcaatgtg actgcactag
     1681 ttacaaattg agtcaaattt tgctgcagcg tgcttgcgat gttatccata tcattgattg
     1741 ctcgagacat gatatcccca tttgtatgtg tatcataata ggaaatcggt acacgattca
     1801 ttttggcttc caaatcatga cgtaattcat aaactgttcg ttgggaaaca cgtgtcataa
     1861 taaattgctg taaaaagttg aatacagcag aaatcaaata catcacgatt actgtcgcaa
     1921 tgatctgcgc gattttatca aagtctatcg gaaattttgt tacttgttgg ccttgttgca
     1981 tctgttgagc gccagccatt acacctttga atatttctgt ggttgctttc cccaagacct
     2041 ttggtgtttg gatttggaag atcacggctg ctattgctag tacaaataca gcgatgatcg
     2101 caaccatacg tttagacata tagccaaaca atcggcgtac cgttccccag aaatttttag
     2161 gcttttcacc ttttacaccg agatttctgc caggtccacc atgtcctgga ccgcctgctg
     2221 gtccatgaga gggacgtgtg tttgttttac tcatgctaag tcctcctctc ttagttgtga
     2281 atgaacgatt tcttgataag tttcgtttgt tgctaatagt tcttcatgtg ttcctttgcc
     2341 aaccagtttt ccttcatcta gcacaaggat catatctgca tccattactg tacttactcg
     2401 ttgagcaact aaaacggtga tactttcttt catattcgtt ttcaatgctt ggcgaagatt
     2461 ggcatccgtt ttgaaatcca acgctgagaa ggaatcatca aacacataga tatctgctgt
     2521 tttgactaat gcccgtgcaa ttgccagacg ctgtctttgt ccaccagaga agtttcctcc
     2581 tccttgttcc acatggctat ctaagccatc ttccatctct gaaacaaaat ctttagcttg
     2641 tgcgatttct aatgctttcc aaatcatttc atctgttgca tccggcttac catactgcat
     2701 attgtctcgg atcgttccag agaacagaac tgctttttga ggaacaaagc ccattaattc
     2761 tcgcaaatta tattgagtca tgtcactaat gtccgttcca tttatttgga tcgaaccaga
     2821 ttcgatgtca tataatcgag ggatcaaatt gactaaggtc gttttacctg aaccagtccc
     2881 ccctatgatt gcgacgatct cgcccgactt ggcttggaaa tcaatatctt ccaatgcaag
     2941 tttttctgct ccatgatatc gataattgac atgattgaag gccaatgttg cgttttttcc
     3001 agcaaaagaa actgttttag gattttcagg gtcatggata ccgatttttt catccaacac
     3061 ttcattaata cggtcagctg aagcttgtgc acgaggcacc atgataaaaa tagcggaaag
     3121 catcataaag ctgatcaaga tctgcatcgc ataagtcata aatgcaatca gattaccgac
     3181 ttccaaagtc atatctgcta tgtagtgtcc accaaaccaa gtgattgcta cgtttgtacc
     3241 gctcatgatc aatgtcatca ttggcatcat caatgcgacg atcgtattaa ctttgattgc
     3301 cgtatttgta taatctttat tcgctaaatc aaaacgattt tcttcaaagc gtgtcttatc
     3361 gaatgctcga atcactctga cccctgtcaa tccttctcga aataccaggt ttaaacggtc
     3421 agttttcttt tgcatacttt tgaataatgg aacagcaaag tacatgattc cgccgatcaa
     3481 aataatcatt actgggatga caactaagaa gatttttgtc aactgatgat ctttttgata
     3541 agcaagtatg ctcgcgccaa tcaacgtgat tggtgcattg atcatcatac gtaaaaacat
     3601 ttgtgtaacc atttggattt gagtcacatc attggtcgtt cttgtgatca aagaggccgt
     3661 tccatattta tcaaacgcat cttttgtcag actctctgat tttttataga catccgtacg
     3721 caattgctta cccagtttct gtgattcacg agtagcaaaa aatgtatttg cgatagcagc
     3781 taagatactg atcaaagaaa agccgatcat cacaaaacct gtatgccaaa tataatcgac
     3841 atctccttgc gctacccctt tatcaataat attggaggta agtgtcggca aatataaatc
     3901 tgccagtact tggatcacca tgaaaactgc agctgcaatt gctgaagtca aggagatccg
     3961 ttttacgatt tttagcatct agttcccttc tttctattta tatactccgt ttttcagcca
     4021 agaaagtttc aaattgaatt cactgattgc ttgctctact gagtaatctt cagatgttga
     4081 aaataagcga tacgaatgcg caattgttgt aaatacagta ttcatcacta attggatcag
     4141 taattccaat tcatgatcat tttttacttt cagcttcgtc cgatcgatca attcaagcaa
     4201 ctcttttgca tcttgtttgt gttctttatg tggatcgtga ggcgtttttt ggcgactcgt
     4261 atgaggagcg acttttcttg aggcacggta atccatgttc ataaacaagt tacgataaaa
     4321 agaagcattc tcgccttcca aaatctcata aatcatttta gaaaaatagg attcgaatcc
     4381 tttgaataga tcaccatcgg attctttcat tagttgtaac atatctttag agctgtcacg
     4441 tctcattgtc tcaaagtaat aaaaataaag atcctcttta tcttcaaaat actgatagaa
     4501 actgcctcta gggatatccg ctaatttgac gatattagca atcgaagctt cttttaaggg
     4561 tactctagaa aactcgattc tcgcagcctc gattagtctt ttttgttttt ctttaggcaa
     4621 atggaaaaat gtctgtgtcg gcatatggat tctcctttct ttcaagatga cacattgtca
     4681 tatgacgcta tgtcattata acacctccta cctgttttgc aagacatttg catagttttt
     4741 ttcattatca tccgatacat taaaaaagtg ataagaggaa tccttccatt tgaattaatg
     4801 agagttgccc attaaaatag ccacatacta aacaaaggat gagataaatg gcccaatcaa
     4861 ttagtgtcca agacttcaat aaattgcctc tcgatgctga cagcatcgtt ttagatacaa
     4921 gaaatacctt agattacaca aaaaaccact taagccactc gctttctatc cccgctgcta
     4981 tcttgccaag acaattaaaa aatctagatc caaagaaact ttattatatt ctaagctata
     5041 caggcaggcg atcagaagca ttagccgatc aacttactgc taatggtttt cgtgctgttt
     5101 atatcattgg tgggatggaa gctcttcagt cgagtgttgc ttgaatattg cttgttagat
     5161 tcaaaagagg ctgtgacaaa aaatcgtgtc acagcctctt ttataccctg tcttttttta
     5221 gttatttatt cttcaacctt atcaatatga cttgatagcc ttttcttttt cgagtaaact
     5281 gattttgtta gtcatagtta taacaatcat agcaataaaa ggagaaggta agcatatggc
     5341 atctgtttta gtagtcaaag gtcatcccct aactgcagaa gaatcgcgca cagttaaggc
     5401 attgacgtct tttttaacaa gttataaaga aaatcatcca gatgatgaag tgactgtttt
     5461 ggaactttat cgcgatgata ttccggaaat cgatgaagaa ttattatcag gttgggaagc
     5521 attacgtgct ggtgcagaat tcacttcctt atcagaaagt caacaacaaa aaattgctcg
     5581 tttcaatgaa ttgactgatc agttccttgc tgctgataaa atcgttatcg caaacgcctt
     5641 atggaatcta aatatcccaa caaaattaaa agcttggttc gataccgtca atgtagctgg
     5701 aaaaacgttc cgctatacag aaaacgggcc agaaggatta gtgacaggta aaaaagcact
     5761 gcacatccaa tctaatggcg gtgtatacaa cggacaagac tttgcctctc aatatgtaaa
     5821 aggtatttta aactttgtag gcatcgatca agtagatcag ctattcatcg aaggaatcga
     5881 ttatgaccct gaccgtgcag atgagttgat gcaaaatgca ttagataaag ctgctgcttt
     5941 aggtaaatca ttttaaaaat tgagataaat gcatacaaaa gagacagagg aaaaaatctt
     6001 caaagcagca tttgtgcctt ggatattttc ccatctgtct ctttttgtta ttctttctgc
     6061 tttttcaacg gattttcttc ataaagagaa gagattgttg tgtaccattc aaaaacatga
     6121 gtaatcacta ctttagctgc tgcataaaca ggaatcccca agatcacacc gatgattccg
     6181 aacaatttcc cagaagtcag cagaactaat aaaatcgtta ctggatggat tgctaactga
     6241 ttccctagta ctaatggaga gatcaagcgt ccttctattg tctgctctac tgcaaaaacg
     6301 atcagtactt tgatgatcat gaatggacct gctacaatgg ctaaaaagat agccggaatc
     6361 atcgctaaaa atgatcctaa gtaagggatc aggtttaaaa aacccgcaat aatccccaac
     6421 gtgacagcat actctaaacc aatgaccgca aaaccaatca taaacatgat tgccacagca
     6481 aaagccacag ttagttgtcc tcttatataa gaagaaactt gactattcat ttcactcagc
     6541 acattcaacg tcggtttttg catgcgtgtc ggtaaaaatt tgacaaaata aggtgctaat
     6601 tgtttaccgt ctttcaatag ataaaataag ataaacggca tcgtcagcag agccactaaa
     6661 atagtcgcta ccgcgccgac aaaacttcca agactctgaa ctgtactctt agatatatct
     6721 tggatcatat cgcctgccga attcatgaac ttatcacctg ctgtttccaa ctgttcgcga
     6781 aattggttga acagcgggtc ccgcaaaatc tcggttaatt tattatcaat cgtatctaca
     6841 tattgaggga agttactgat gaagctcact gtttgttcct gaatcttagg gatgatcact
     6901 acgctccccc atgcgattag tgcaacaacc aaaacaaaca gcccaatgat actgtaaagc
     6961 cttggtactt ttcgcttttc aaagtaatca actaccggat tcatcaagta atacaagatt
     7021 cctgctaaaa tgataggcag acctacgatt gctaaaaatt gccagattgg tgcaaacaaa
     7081 tatgaaactt ttgtaaataa aaagacgatc agcaatatta acaatacaac aagtaaggct
     7141 gtgactactt gattatttaa aaaccagcgc caaaaccacg ataccggtct gttcttctta
     7201 ttttttcctt ccataattcc tcctgttagt tataaaaaat ttggcttccc aaaccaatag
     7261 caggcagtct atgcggatga agatagattc catttgcaat ttgtccttct tctggacaag
     7321 catcaaaaat caatgatacg tgagctactg tctttaaatt cccattggcc atcggaccga
     7381 cataatcgat cgtatatacc tgatcatcaa aagcaattgt tccgcctgct ttcaaagaaa
     7441 aaggttcgtc ttttgatact tcttgaatca ccgaatattt tcgtaaggca gctgttgctg
     7501 ttttatcgaa caaaatcagc atgggttctt ccttgtttat tgcttctgtc ccaatttctg
     7561 taatctttgc ctgaatcatt gcactctctc ctttaattgc tttcaaaaac agtataacac
     7621 attctcattc tttagccgtg tggaataatt atgtgataga ataaaaacga aaactatttc
     7681 attacaatta ggaggaatca gctcatatga tcgaaaagat aattttagga acctatacgc
     7741 gtcgcgaaag taaaggaatc tatacgatcg accttgatac agaaaaagaa gaattagcca
     7801 acctttcatt agctgttgaa gaaaatagcc ctacctatgt agcaaaaagt aaagctggca
     7861 atctttacac agttactagt gtggatggat taggtggtgc cggtgcgtac gataaggatt
     7921 ttcatttttt gaatgcggta acagagtctg gcgctccttt gtgttatgta gcggttgatg
     7981 aagcacgtca attactatac ggggcgaact atcataaagg tgaagtaaat gtctaccata
     8041 tattaggaga tggcgaactg aaagcagcag attcaatatt ccatcaagaa gcgacaggtc
     8101 ctcataaaaa ccaagatcat gcgcatgtcc actatacgga tctgactcct gaccaacgat
     8161 tagtcgtatg tgatcttggt acagatcgtg tgtatactta tgatgtgtcc gaaaacggca
     8221 aattgaatct tgtaactacc ttcactgcag agccaggaac aggccctcgc catcttgtct
     8281 ttcatccaaa tggacaattc gcttatttat ttggtgaatt agacagcaca gtccgcgtgc
     8341 tttcttataa taaagaaaca ggtgcttttg aagaattaca gaaaatatcg accttgccag
     8401 aagaattcga cggtgaaaat ggaggggcag ctatccgcat ttcaaatgat ggcaagttcc
     8461 tttatgcttc taaccgagga cataactcga ttgctgtatt cgcagttttt gaagatggtt
     8521 cccacattca aaacaagcaa atcatctcaa cagaagggga ttttccccgc gatttcgcac
     8581 tagatccaag caataatttt gtcgtgtgtg ccaatcagaa caccgataat ctgacattat
     8641 atcgccgtca tcctgaaaca ggattactgg aattactgaa aaaagatgtc tatgtaccag
     8701 aatgtgtctg tgtttatttt gatcgttagt aagaaaagga gaatgaaatg aatcagcaag
     8761 acgtattaga cagactacgg gaagaactaa atattccttt ctttgatggg aaaatcgaag
     8821 ataaagagta tagcgaagag gaatatcaga aactcaaagc cgatttacag aactactttg
     8881 aacaatatgt acagaatgta gaaaactaat cagctttcca tactatctga aaagaacatc
     8941 aggacaagac ttttccgtct atcctgatgt tcttttctac aaataaaaaa ccgccgagat
     9001 ggttagactc gacggaaaag gagtatttac tcgattttga gtaagtacaa ggttgttgtt
     9061 ggttataggt atgtcattat cataaaggat tttgatcact ctatcataaa ggtaatatta
     9121 catttatatt agataactct agtttttatt gttatcaaag atttctttta ttttttcaac
     9181 aacaaattag tcattttttg gatggcagct gtcaattggt ctgtcgcttg gtcttgtttt
     9241 gcttcagatg tattgaatag atcttcatct acccctgatt caagatcgat actttggatc
     9301 atttcttcac agcctgctac atatgaaaga taagcttttt caaaattttt atgaatgccc
     9361 attgcttttg ctggtggacg caaagtactg actttcataa gcattgcccg atattttttt
     9421 gttccttctt caaataacag gatgatctct tcaagagtcg atttgtccag ttcacttgtt
     9481 tttccttcat caattgcgtt tctgatcact tcatatttcg gatgcattcc ttctccgatt
     9541 gcttctgttt ccttgacgat ttgatcgatg gactgcacat aatagccaat attcggtaac
     9601 atgttcgttt tcctactttc tccattgttg ttattattta ttctacttgt ttttatcctc
     9661 aaatgacaag tacttgaaaa aatttaatgg tttctataca gctcgccatt cttattcgtg
     9721 ctcaaagaaa ccatctgcta aactatattg tttccctgcg acaatgtcat attggatcaa
     9781 ttgatattcg tcgtaaatct cttgttgcga gcgattgaat tgcgcttctt tataccactg
     9841 tccgttttga tagtacagtt ctcgttcaaa cgccggaagt tcctgttcta atgataaaag
     9901 cagctgataa aagccatttg ttgtctgatt cgtctgttcc attagattcg ctgcaaaata
     9961 aaacggactg gtgattgcat cgtgagtcgt ttgcttttct aattctcctt tactgtcgaa
    10021 cataagaaat tgtgtttcat gcaatgtttg tttgtcattt tttgcttgga tcgtatctga
    10081 atagatccct ggaagatgat ccccccaaaa aacaactaaa gtccttctag aaagattctt
    10141 caattcttcc gtgaactgct tcagtgccgt gctgctatag gaaatatctt gcagataatt
    10201 ctctaagtca agtgtcccac ttccatctga agtttttgca ggatagtcta gattatcata
    10261 ctttccatcg tagggcatat gtgtctgcat cgtgactaaa tgaatgaact gaggtgtttt
    10321 ttcttccttg agaagtgtca ttacttcttt gtaagctgac tcatcagaaa tatatggatt
    10381 attctctatc gtatccgtat aggtcatcgt acgctcgctg atgaactggt caaatcctaa
    10441 tgtctgataa acatcctctc ttttatacat cgaagtattg tatggatgga tcgctgtcgt
    10501 ctggtatgat tgtgcgttta acgctgaaac gatagaagga agctgatcca tttttggtac
    10561 aagcatcgtg taaggggtgg tgagttgcgc attaaataac gccatagaaa aaccagtcaa
    10621 tgcttcaaat tcgatatttg ctgttccacc gccatagttt tgagaaagca tattgccact
    10681 atatgtttga tcagctactt cataataatc tgccaatggc tcaccagata cttctacgcc
    10741 gtttaatcga ctaggatctg agaaactttc actcatcacg aacacgatgt tcggcgactc
    10801 ttcctctaca gttttatttt tttcatcggc tagcttctgg tatttttcag tgatttcttt
    10861 gatcttttct ttcgaatatc cttcaggttc atccattggt tctaccttca gattgtacaa
    10921 aaacccaccg ataaaaccag tgttatagta gttcattttc tggctgtaag gtatccatag
    10981 tgccgtttta ttatatgctt ttcgcaacaa attatcggga ttgttgaaat tactgatata
    11041 ccctagcagt ccaattgcca caagcagtgt cgttaatcgg atcaattgga atcgccttcc
    11101 ttttttcagg cttcgaaaca tgtaccagca aaacaatcct atcacggttc ctgcggcagc
    11161 taagatcact gtaaacagca tagtgccggt catatctttc agcaatccga tttcagtgat
    11221 catcttcaaa tcgtctggat aaatcggttc ttgccgataa ctcattttca tataatttgc
    11281 aaaacctaat acaccgatgg ctacgacata cgtaagggaa cctaaaggga cagatcctgc
    11341 tagtgcaatc agaaaaataa gaataatcag caagacgaga caggctaaga agaatttttc
    11401 cgtatgccaa gaaaaagcaa atttcaacgc aaggtctacg gatagatcat tttgacacca
    11461 ctgcaaaaat aaattactaa aaacaacaag aaaaaagata ctgatgattg ctgctgttcc
    11521 ttttaaaatc tttttctgtt tggtcgaaag tctgcttata tatcttttca aatgcttgct
    11581 ccttcatttc tatcaataaa agaatgttgc ttctcttatt ttagttgtac gaaattcgaa
    11641 agtaaagaac ggaaagaaga attaatgaaa tccaaatatt ctctttcttt atggcctttc
    11701 tggtttcttt aataagcgga cctttcgtac tccagaaatt ttttctagct gttgtttcac
    11761 ttgattgagc tgttcaaaat cagtactgtc tatgtcaatc aatatgtaga catgctcatt
    11821 cttatggtta cgcgatatat ctgcgatcgc tagctcgttc tcattcagca gagaaaatat
    11881 ctctgctaat atattggttt tcttttgata aaatatagtg aatcgaaacg gcgcttggaa
    11941 aaacattcgt gcggatggat aattgaccgc ttctctaacc gtaccgaaca aaagataatc
    12001 ttttaaggct tcaactgcca aacgtccgct gtctgccaaa gcttcctgag tcgatcccct
    12061 agatgaggca acataagtat acggtcattt tctaagaact cttcttctgg aaaatcagtc
    12121 aagtatttag ccagatgttg tttttccaat gcttgcataa cagcatattt atcaacaatt
    12181 tcacttcttc ctacattgat aagtacagca tcttttttca ttttttctat attcgcctga
    12241 tcgatcaatc cttttgtatc ttctgtcaac ggcagcatga ctacgatgta atccgaggta
    12301 gaaagaactg tttctaaatc tgcttgccga acataatcca actgggcatc gcggatagaa
    12361 taacccaaga catccatccc taagctatag caacttagcg ctacttttgt gcctatcgct
    12421 ccaagaccaa gaagaccgat ggttttgcct tgcaattctc gtcccacata agcacttctt
    12481 ttgttttctg cctgttctaa aatattagga cctgttagtg tttgtaccat acgacttgct
    12541 tcgattatcg gacgggaaga taagagcaaa cagcacaaaa caagctcttt tactgcatta
    12601 gcatttacac ctggtgtatt catgacgatc gtcccgtttt cagaagcttt ttcaacgttg
    12661 atcgtgttca ccccaacacc agctcttgaa attgctagca aatctggtgt aagccattca
    12721 tctttcactt tcgtactacg aataactatt ccttcggctg gcaaatcatc tattattttg
    12781 aaatattccc cttgtaactt ttctatctct tgttctggaa tagcatcaat caaatgaacg
    12841 tgatacataa aaccccctcc ttactaacaa gtatctcgaa atttctgcaa ttagacaact
    12901 gtttaccatt tgacttccta gaagcaaaaa gagcagttag agcagagtag gctgagtgat
    12961 aaaaataagg ctatgacaag ttactgccat aagccttatt ttcgagagca aaatattgag
    13021 ggtgagatat cttgataaat ggtgttccgt tagctgacat tgataataag actaaatttc
    13081 aaaaaagttg aaaagcaaat ttcgtcaatc tcttcttatt tcttaaaact aagtggcttt
    13141 ttcacaacct ctttttatct aataaccact gtttccaact caactaattt tgcccacgtt
    13201 tcaatttgat ctgttgttag atttagcgag acaacggtat gatgaccgcc acctgcctga
    13261 atccatgaat ggacaccttc atggaaattc ggtttagttt tccataacac acgtgcaaca
    13321 ggtaaattag gtgcttcagt cgttggttca aaggcttcta cttcattgat cagcagttta
    13381 taatgcgtac cgaaatctgc catcgatacg actacccctt ctcctgcttt cccatcaaat
    13441 acaagacgcg ccggatcttc tctatctccc attgataaag gagaaacaac gattcttggt
    13501 ttcgtcgcag caagcgtcgg atctacttcc atcatatggg attccaagat tgcttcttgg
    13561 ccactagcta gctcgtaagt ataatcttcc atgaatcctg tatctttccc tcgagccatg
    13621 attttcagca accgatcaat cgctgcagtt ttccagtcgc cttccccagc aaaaccgtat
    13681 ccttctgcca tcaaacgttg tacagctagt ccaggtagtt gtttcattcc atacaggtct
    13741 tcaaaattag tcgtaaaagc gttatagcct ccttcttcta agaaacggcg aataccgatt
    13801 tcctgctgag cttgtacttt tacatgttcc tcccatgtct tttcttcata atcaccatag
    13861 tcgaaggtat acaattcctt gtactcttca aataattgat cgacttcttc gtctgatacg
    13921 cgatcgatta cttgtacaag atctccgata ccaaaataat caacagtcca accaaattga
    13981 atctgtgctt ccactttgtc tccttctgtg acacccacgt ttctcatgtt gtcaccaaaa
    14041 cgagccactt ttattccaaa actttcatta taggctactg caacatccat ccaatcagca
    14101 atctcttttt ggatctcagg tcgtttccag tagccaacga cgattttatt ttgtttattt
    14161 aatcgagcat tgatgaaccc atattcgcgg tcaccatgag cggattgatt taaattcatg
    14221 aaatccatat cgatcgtttt ccaaggaatg ctttcgttat attgcgtagc taaatgaagc
    14281 aatggttttt gtagaagctt tgtcccgcgg atccacattt ttgctggtga gaaagtatgc
    14341 atccaagtga tcacacctgc tacttcttct cgataattga cctctttcat gatttttgtg
    14401 atcgtatcag cagtaactgc taattcttga aggcggattg gataaggcaa ttggccattt
    14461 tcattcagtt catcgaccat ttcttgtgca tgtttcttta cttctcgcag tgcttcttcc
    14521 ccatataaat gctgtgatcc tacaacaaac caaaattctt tttctccaat attcaacata
    14581 attagccact ccttttattt gttttgtccg taatacgcat tttttccgtg ttttcgtaaa
    14641 taatgtttat ctaaaatccg ttgaggcagc tcttctgaaa atgaattgag ctgtctggta
    14701 aataaattca tcttgcatac ttcatctagt acgactgcgt tcattaccgc actttgcgca
    14761 tctttccccc aagtaaatgg accgtgtccg tgtaaaagta cccctggaat tgctaaggga
    14821 tcaattttgc gttctttgaa tgtttctatg ataacttttc ctgtttcata ttcgtacccg
    14881 ctgtcgattt cctgttgtgt caaaaatcga gcacaaggta ccgatccata aaaagtatct
    14941 gcatgcgtcg tccccatcgc aggaacatcc aatcctgctt gtgcccagat cgtcgcccat
    15001 gtcgagtgag tatggacgat tcctccgatt tgaggaaatg aacgatataa aacagcatga
    15061 gtaggcgtat ccgaagaggg attgagttta ccttcaatga tatgattgtc caagtccaca
    15121 acgaccatgt cgtctgctgt catctcttca taactgactc cgctaggttt gataacgaaa
    15181 tatcgtgttt caggatcaaa agcgctcaca tttccccatg tatatttgac gagtccgtgt
    15241 ttaggcaagt caagatttgc ttggtatact tcttctttca gttgttccag cattttctat
    15301 cactcctttt tcttgcaatt gatgaatcgc tgttttttca atgtcgatcc cctcacggta
    15361 acgtctcata aatatctgat agcctgctat ttcttctgct gaaggtgtga tggtgattcc
    15421 ttggtcttgt ccaaacacgt cttctgccaa gaagttttct aaagacttcc catgtcgatc
    15481 catcatatag gctgctaata acgcgattcc ccacgctcca ccttctccgg ctgtgtccat
    15541 cactgtaact ggtgcctcca tagcagatgc tagaatccgc tgtccaactt ctggtgtttt
    15601 aaaaatcccc ccatgcccca ctaaacgatc aatttggaca ttctcagatt tcaatatatc
    15661 cattccgatt ctcattgcac caaaagcact ggctaaatgg atccgcatga aattcgctaa
    15721 atcaaaacgg ctacctggtg tacgtacaaa taatggcctt ccttcattta ctcctgtaat
    15781 gttttcaccg gaaaaataac cataagaaag taatcccccg caatccgtat cgccttctag
    15841 cgctttgttg aataataccg tgaataattt atctctgctt atctccatac ccaaagaatt
    15901 ggtaaattct tcaaaaatac tgacccaagc attgatatcc gaagagcagt tattcgtatg
    15961 aaccatgcct actagttctc ctgaaggtgt agtgaccatg tcgatttccg gatggacatt
    16021 ttctagttct ttttctaata cgatcatagc gaaggcggaa gtaccagccg aaatgttccc
    16081 tgtccctttg gctacactgt tcgtagcgac cataccggtt cctgcatccc cttctggcgg
    16141 acaaaccggg attcctggtc tcaaatttcc cgtagggtcg atcagataag cccctgattc
    16201 gcttaacttg ccagcttctt ctcctgctac tagaacttta ggcaaaatgt gatccaagtc
    16261 aatcgctacg tgcgcttttt cgattaactg accaaaaata tttttcattt cttgatggta
    16321 ggttttcgtt gaaggatcga taggaaacat gcctgatgca tctcctacgc ctagtacttt
    16381 ctcaccagta agctgccaat ggacataacc tgcaagcgtt gtcaaaaaat taatttcttt
    16441 cacatgttct tctttattca aaatagattg atacaaatga gcgatactcc atctctgcgg
    16501 aatattgtat tgaaattttt ccgttaatat tcgttctgct tcttcagtga ttgtatttcg
    16561 ccaagtacga aatggtacta gtaactgttc cttgctatca aatgctagat aaccgtgcat
    16621 cattgcactg aacccaatcg acccgattgt cgtaagattc tcgccataga tttcgaatac
    16681 ttctgctgcc atttttcggt aactgacgcg cagtcctttc caaatatctt ctaatgaata
    16741 ggtccagata ccattttcta gtttgttttc ccactcataa ctaccagcgg caataggagt
    16801 aaaagatgaa tcaatcaata cagctttgat cctagttgat ccaaactcaa tccctaaaga
    16861 tgtacgacca tctatgacat ctaacatact ttgcgcattt acttccttca taatgattta
    16921 actccctcct tctcgtcaaa cctgatcaaa ccgtcttttt agtagcgctt acaatgatta
    16981 ttgtatactt ttgtacgtac atgtcaagca gttatttaca agaatacaat aaaaaacact
    17041 tgttttgtac gtacataagt tataaaatga aattgcttac aaaatctgat caaccgcctt
    17101 ctcgaaattc tagaaggaga gaatacttat gaaaaaagaa aaaaaagttt cctcatcgtt
    17161 tatttatttt tttggcgcat ttggcggcat tctatttgga tatgatattg gtgttatgac
    17221 cggcgcctta ccttttttac agcatgattg gggtttggca ggtaaagctt ctttgatagg
    17281 atggatcacg tcttcggtca tgttaggtgc gatccttggc ggctctttat ctggtcttct
    17341 ttctgataag cttggacgac gaaaaatgat tcttctttct gcacttattt ttatggctgg
    17401 ttctgtcctt tctgcatctg cccctcacaa tggttcttat tttctgattg cagcaaggat
    17461 tcttctaggt ttagctgttg gcgctgcatc tgccctagtt cctgcttata tgtctgaaat
    17521 ggctcctgct agactaaggg gaagattgtc agggatcaat caagtgatga ttgcttctgg
    17581 catgctcctt tcctatgttg cagattattt gctaaaaggg ttgcctgaaa cgatggcttg
    17641 gcgcgtgatg ttagggctcg ctgctgtccc cgcgctgatt ttatttttcg gtgttttagc
    17701 tctaccagag tcaccgcgtt ttcttatgca atctggccgc ttggaagagg caaaaagagt
    17761 attgaactat attcggacac caaaagaagc tgaacaggaa ttcgaacaaa tccagctgaa
    17821 tgtcaaacaa gaaaagacaa ccggaacttc ttggcatact ttgtttttag aaaaatatcg
    17881 ttccttagtc attgccggta ttggtgtagc agtattccag caatttcaag gagctaatgc
    17941 tattttttac tatattccgc tgatcgtaga aaaagcgact gggcatgcag cgagcgatgc
    18001 attgatgtgg ccgatcatcc aaggaatcat tttagttgct ggttctttgc tctttttggt
    18061 tattgcagac aagttcaatc ggcgaacttt attgaaaata ggcggtagcg tgatgggact
    18121 ttctttcatc ctaccagctg ttttaggaac tgtactggat gctcatacaa attcgttgtt
    18181 gattctcttg tttttatgta tttatgtcgc attttattct tgcacatggg ctccactgac
    18241 atgggtaatc gtaggcgaaa tattcccact agctgtccgc gggcgagcat ctggcctagc
    18301 ctcttctttt aattggattg gttctttcct agttggcttg ctttttcctg tgatgactgc
    18361 ttctatgtct caagaaatcg ttttcggcat ttttgggatc atctgctttt taggggtttt
    18421 atttattcaa gagatcgtac cagaaactcg tgggaaatct ttagaggaaa tcgaacaaag
    18481 tgcatctaaa aaaacttatc ctaaacgtat ttctatccat cattcttaaa gaggttgtga
    18541 cagaagtggt cagcttcaag aaataagaag gaaccgctta gtttccagtc acaaggaaca
    18601 atttcaaaaa acagcttctc atgtttgatg aatattgtga cttgtgacca gttggtagga
    18661 gaacaccgtt tagtggagag gtggtgaaag aaacgttcag ccttgagcga tgacaaaagc
    18721 tctcacaaac attgcgacga gtaaccgtag gagcaataag aggcggtaaa aacccgtctc
    18781 ttatttttcg ttttctttca ctgtttcccg ttcaactagt acaggttcat agagaatatt
    18841 tggctgtgcc tgtccttttt caatcgttgt gacgatccac cgtgcggcat cttgtcccaa
    18901 cttttctttt ggatgcgtca atgttgtcaa ttttttattc cctaaatggc ttaaagaaga
    18961 atcgtctgtt cctacgatcg ataggtcatc tggaatatga tagccatttt ctgtcaactt
    19021 atctagcatt aaactagcta tttgatcgtt atagcaaatg atgccatcta aagcttgttc
    19081 ttctttcaac cgttgtactg ttttttcagc gattgtttct cgtgtttccg tggtgaaagt
    19141 aatgatatct tccgtcaaaa aacgcacatt atactgttca cacgcgctga taaaaccttt
    19201 catcctgtat tttccttgaa gatcgtcgat ttttgtgatg aacaacagac gctgatgtcc
    19261 tttttttatc aaatattctg ctgccataaa accgactttc acatcatcta aacaaatata
    19321 agaggcattt aactcttcat aagcagcatt gatcatcacc atcggaatgc cttgttctct
    19381 caaagcaaca taaagtgcta agtttgggtt atactgatta cttttcgtag gctcaacgat
    19441 caaaccatct accccataag caatcatttt cttcaaacac tctttctcca attcgtgatc
    19501 gttattcgta ctggctaaaa gcaatgaata cccagattct cgtaattcat tttcgattcc
    19561 tcggataatc gacggaaaaa tataatcaga gagatatgtg gtgatgacac cgatcgtttt
    19621 tgttctgttc aatggtgttt tttctttctt atagctgtca tctacatatg tgcccgaccc
    19681 tttttcttta cgcagatagc cctcatttac caacacagcg attgcttgcc gaatcgtatg
    19741 tctgctgacg tcatatattt cttgcagctt gaattctgag ggaatggttg tatttacttc
    19801 gtattcctca cgaaggatct tttgttttat ctcttctgca atatcttgat attttgccat
    19861 ttgccttgcc cctttttatt tgtacgtaca tatatcataa cgagtctgca aggtttattc
    19921 aagcaaaaat caaggtttct tgtgctcata aggcatacgg aacaggttca ccaaattctg
    19981 gaaatccttg ctcattccac gtaaagcatt gcgcgttcgc gtgccgatta ggattgtcta
    20041 acggatcccc tttttggttc ttctctggtc gagcatgata gatcaaaata tcaaagcacc
    20101 cgtcttctga aaccgtaaac gagttgtgac caggaccaaa ttgcttattt ttttcagaag
    20161 aaacgaatac tggctccgat aatttatgcc atgattcttt taataaaaga ttcttgtgct
    20221 catcagccca cagcaggccc atggcatagt tttcatctgt tgcactacca gaatacgtaa
    20281 tgaatatttt cccattgcga gtaaggactg ctggaccttc atttactgaa aaaccccgtt
    20341 tttcccattc atactcaggt atagaaagga ggatctgctt cccctttaac gtccaaggat
    20401 tatccatttc ggatagataa agattagaat tcccagtaat ttcaggatct tgttgggccc
    20461 acacataata tagctttcct tgagaagaga aagttgttcc gtctaaactg aaagtttccc
    20521 ttttcgtttt tatttgtccc ttttctatcc aatctgtatt cattggatct atatgctgat
    20581 tttcaagtac gaacatgcgg tgatgatgag attcatcacg tatagtttta tgattgcttg
    20641 cagcaaagta aatataccat tttccattga tataatggac ttcaggtgcc cagatcaatt
    20701 cactcatcgg tccttcttca tgagcctgcc atacaatcag gcttgcagca tgttctaatt
    20761 cattcagtga ctttgccctt cttaattcaa tcacttggta gcctggaacc gatcctgtaa
    20821 aataataata accatctgta tgtttatata cccaaggatc tgcccgttgc cgaacgatag
    20881 gattattata aagtgtcttc atcataacaa atttcctcac attttcttta ttctaattac
    20941 attccaagag tatttttcta tccctacatt taattcgtca tgtttttcta ttgaaaaata
    21001 gtttatttca ggttttattt tttctggttc ttcacgtgta tttttgtcca acagatcgct
    21061 actggttaat gtgtagtgct ctccttgatc tagcaaattg aaatcatgtt caatcgttat
    21121 cttttgttgt tcatttgtgc gattgatcaa gaaaatagtc agctgattgc cgttatctac
    21181 gatcacactg tccacataag gaacattttt tatcttttca caatcataaa cagggcaaat
    21241 gatttgttgt ttcaatactt gacctttagc atatttcgat aagtgttgca tcacataata
    21301 agttggattc ctccatgctt gtcctttagc atctgtcagt attaaaggta ttgtgtttac
    21361 taacaatgat tgacaactaa tctttacgcg atctgcgtgt cgtaaaatag ccagccctaa
    21421 tgaaccaaat aataatgtat cttccatagt aaagtaacac caatcgatgg gactaccgac
    21481 ttcaaaatcc ctatgtcttt tttccggttg ggcatgtaca ttccattcat caaacgctag
    21541 atacattgtt ttctcacttc tcaaatatcc ttttacataa tcacaagtag caacgatttc
    21601 ttcaatttga cgatcgaatc gataagcttt tgcaaggtat gtcgctgtat catcacgctc
    21661 atttttagga ggtttagtca agtcttcttc atcatactta tcaatatagt tatgcaaagc
    21721 taaataatct acattctcat aagcttcttc taatgcgata cgatcccatt cagggtacga
    21781 tggtaaacgt ggcgtagagc ttccaactaa gattgtttca attgaatcat ccactaactt
    21841 catgactttt gaggcttcat ttgctaacct accatattca taagcactct tagcccccac
    21901 ttgccatgga ccgtctagct cattccctaa acaccataat ttaacgccaa aaggcttttt
    21961 ttgcccattt tttattctta aatcactcca gaaggttcct tttggaaaat tacaatattc
    22021 tactaaattt tttgcttcat caattccttt tgttcctaaa ttcaccgtga atatcggttt
    22081 cgcttgtaca tgactgatcc atttaaaaaa ttcgtggagt ccaaaagaat ttggttctat
    22141 acctcgccat gctaaatcaa ttttttttgg tcgttgttct ttcctaccta cagtatccac
    22201 ccaatcataa ccagatgtaa agtttcctcc aggatagcga atgacaccta attctaattc
    22261 ctttacataa gagaggacat cttgtctaaa cccgtcacca tcagctgttt catggcccgg
    22321 ttcataaatc cctgtataga cgacacttcc catatgctca acaaatgagc caaacagccg
    22381 agaactgatc tctcctaccg taaaatcctc tctgattttt actttcactt cttcgtccaa
    22441 cagctttccc tacctttcat ttcctatagt caagataata gatacaagtt tccatacgcc
    22501 accagcaaag ataaaaataa aaggagggaa aagaaatatg agcagtacaa atcctaataa
    22561 caatacgcaa caaagaatat ttctcaaaaa atcaaaaaat actttccatg cactttgttc
    22621 aaaaatttgt aaaaatgtga tatctttgtt ttcagaaatc acatatatcc aggataaaag
    22681 aaacatggaa taaataagta aaactacata tccactaatt gaaagtacga ttgtctgttc
    22741 ttctaataat tgattaacaa gaaaataaat aatgatcgtt aacagactaa ggaacaagtt
    22801 cttaccaaaa tgttgcttta ttccttgaaa gaaaaaataa atgatatttc ctgatccctc
    22861 acctttccat tgcgccatta cataaaaaac agcacatgta gcaggcacga tagtaataac
    22921 aggcaaacac acgattaccc agagaatacc taataagatg taatcagaaa ccacaatcaa
    22981 ctgtccataa agtttagtct caactatttt catcttcctc actctttcat tccagacata
    23041 gctgttcctt gaacaaaata tttctgtgca aaaatataaa ccactagtaa tggcagcatt
    23101 gaaataaccg ttccagccat tacaggtcca taaaaagaaa tgttctgccc tgtaaataaa
    23161 gctaaccctg ctggtaaagt tctcattttt gaactacttg tcaatatcaa tggataaagc
    23221 aagtcattcc aactattcat cagcgtgaaa atccctaatg ataaaagagt aggtttgctc
    23281 aatggcaaca tgattttcaa aaaaatatta aactcactta atccatcaat gcgtgctgcc
    23341 tcttccaaag aatctggtaa agtaataaaa aaggaacgca tcatgaagat accaaatgca
    23401 gttgtcatac gaggaatgat caaacctgca taagtatcaa gcatgccaag caaattgact
    23461 tcaataaata gaggaatcat gaagacttgg aatgggatca tcattgtcag caaaacgaaa
    23521 tagaacaaaa ctgattttcc tttgaaacgc atacgagcaa atgcataccc cgcaagagaa
    23581 tcaaagaata cagaagtgat cactgtccct ccagcaaaaa tcactgtatt tttgatgtaa
    23641 tctaataaag ggatcgttgt ccaaacctta atatagttat ctagggtata gctatctgca
    23701 aatattctag gcggaaatga gataatgtct ttttcgtgtt taaaagaaga aataatcagc
    23761 cacaaaaaag gaaacgcaat aaccaacaat ataactagca tactaatcgt tcgaatgatt
    23821 tttttaccac taactgcttt tcgactattc acagtaactg taatcgagct ttctggtgcc
    23881 atacaggttt ccctcctatt tcatttgttt ttctttccgt gacatgtaca cattagtgga
    23941 gatagctata gttgcaacaa tgataaacaa aaccgaagac agtgctgatg catagcccaa
    24001 ttcataaggt gctgtaaatc ctcgactgta aatatattga acagctgttt ctgtcttaaa
    24061 ttgtgggcca ccagcggttg caacatatac ttggtcaaac acttgcatcg aaccaatcag
    24121 attcgttaaa atacaaaaac ctaaggaggg aacgatctga ggtagcgtaa tgttaaaaaa
    24181 ctgctgtcga tttgttgcac catccatttg agcagattca tacaaagagg gagagattcc
    24241 ctgaatagca gtcaagagaa tcgtcatagt aacaccaaaa ttcttccaaa cagtcatgat
    24301 ggctaccgtc ggcatagcaa gggttggatc gcgaaaaaat tgagggatcg gcaagccgac
    24361 ttgtgaaaac aagtaaggaa tcgcccctac attcggatca agcaacatag aaaaaataat
    24421 cgcaattgac gtcaatgaac aaataactgg aatgtaaaat gtcgaacgac aaaaacgatt
    24481 gaatctggta tttttagata atgctactgc tgctaataaa ccaacgataa tttgtgttgg
    24541 cgtttcgagt acggtgaaat ataaagaatg aatcaacgaa ttaactgctc gttcatcact
    24601 aaatatacgt gtgaaatttt caaaacttac aaaatttctt gatgtaaaga aaatattcaa
    24661 atctgtaaaa ctaatagcaa atgttcctaa aagcggtatg acagtaaata ataataaaac
    24721 taaaaaagat ggagttaaaa acaaatacgc agcacgatca ggacgattcc aatatttggt
    24781 gaatccgttc atatagtcca cctcatcttt cattttgata aaatatacaa tcttggttta
    24841 aacgaatctg aataagatat ccgaacaatc gctgtgattt ttgcaataac acctatctgc
    24901 aaatactagg tcgatcattc agatatctgt atcttgccaa cagtctatcc tgtataaagt
    24961 tctgtactat ttttgcagta attgattgat ggcatcatct gcatcgttca tgagtgtctc
    25021 aggatcttct cctgctaata atttttcaat cagtggattg atgatatcgt tattgatcgc
    25081 agagatttgg gtaaaacctg gtaaatatgg ctctgcatag tccatttgtt tcgataattc
    25141 agatacaata ggatcatttt ttacttcttc atcttttgct actgaatcca agtaggcagg
    25201 aaaaccattt tcgatactcc attttttacc aatttcagtt gtattccaat atttcaaaaa
    25261 ctcatagaca gcttcttttt tattattatc tgtacttgcc ggaatggcat atcctacgcc
    25321 atctaaaata gctgtttttt tgtctgaatc actcccttgt ggtaaggtag tcacattata
    25381 atcaatctcg tttgctttca atccgttatt taaccatggc ccattgattt caatggctag
    25441 ttgaccagca ttcattaaat tgtccatttc ggcgccagaa atcgattctg gaccagtctt
    25501 gtctacatgg atcatttttt ggatcgcttt taacgtttta aggttctcag gagacgcaaa
    25561 ttcagactta gtataatcat cgttcactaa tttaccgcca tttgctaaca tccagttata
    25621 taacggtgca gttccatcct tattgaagac aaaacctgat acatttttac tactatcagt
    25681 taattttggt gccatttctg tcaattcttc ccaagtagta ggtggttgtt cagtatctaa
    25741 tccagcttgt ttaaacaaat cattattcca atacatatac atgccttgta cctgcatggg
    25801 gacaaaatac tgtttacctt ctacttcacc taattcaata gccgtttctt taaaatcaga
    25861 tttatctact ccatcatatt cccagaaatc gtctaatggt tgtaccgctc cgttttctac
    25921 atactgtgca aaatctggat aatttaatgc aataaagtca ggtgcttttt tagaagaaat
    25981 cgctgtagga agtttttcat tcaaattggc ccaagtcatg atatccattt caattttgat
    26041 gtttttatca tttgttttat taaattcatt aacgatttct tgcaatattt caccatcaga
    26101 tgcagtaaac ccattccaaa aagtcaaagt cacttcttta tcagaatcag attctgctga
    26161 acttccgcca ccgcaagctc ctaaaatacc tgttgcagct accaacaaag agccaattaa
    26221 ccatttttta cctttcattt tttgttcctc cttcatctga taaacgacat ctgaccacat
    26281 tccaagatag cggtttcaat aaacaattga ttttcccatc ttcagctttt acatttgttg
    26341 aatgacttgg ttttacttgt tcacttttag gaagattagt tgcttttgta tcaaatccac
    26401 tcatttcaga aaaatcaatc actccctcta atgataattc agacaagtga acagttagat
    26461 tcatttcttc ttctccacgg ttaactgcaa atatgacaag ttctttgtca tttattacgg
    26521 ctatactttc taaatacggc acttcttcaa aatcttccgt cgcatagttt gctgaacgaa
    26581 tttgtgggct taacacctct ccttgtccat acacagcagc ttgcataaat ggataataga
    26641 tcgtctgtct ccaagcttct ccgtttgttt ctgtcctgat tggtgcaata acatttacca
    26701 attgagcaag acaagcaatc tttactctat ctgcatgttt caataaagtg attaatagac
    26761 agcctaatag taatgcatct tcaaaattat agttatcttc tagtaaagga ggagcaatct
    26821 gccaaggcgc tgcttgctca tcttgttccg ctgaatgata ccaaatattc cattcatcaa
    26881 aggacaggtt gatctgcttc ttactatgtt tcttcgcctt gactgtatcg cacatcgctg
    26941 tcacttcaga aatgaaccga tccatgtcta aggaacgtgc aagataatga gaaagatcat
    27001 ttttttggtt gccataatat tgatgcagag acaaataatc tatttgatcg tacgcttgat
    27061 caagaactgt taactcccaa tcaccaaatg taggcatttg ccgattcgaa cttccgcata
    27121 aaaccacctc aattgaatca tctacgagtt tcatcgcctt agccgtttcc tctgctaacc
    27181 gtccatattc ttccgctgtt ttatgtccaa tctgccaagg accgtccatt tcattcccta
    27241 aacaccatgt cttaattcca aaagggctct cttgaccatt cgaacgcctt aaatcactcc
    27301 aataagttcc tttaggaaaa ttacaatatt cgactaaatt acgagctgca tctactccac
    27361 gagtacccag attgacagcc atattgattt cggcccccac atgctgacac cagttataaa
    27421 attcatgaat cccaacttca ttcgtttcaa ttgtccgcca agctaaatcc aaccttctag
    27481 gtctatttgc tatgggccca atactgtctt cccaacgaaa tcctgacaca aaatttccac
    27541 caggatatct aatcatggga atgttcaatt cttttaccaa gtgaatgacg tcttggcgaa
    27601 atccactttt attagctgat ggatgatctg gctgataaat cccttcatat actgctcgtc
    27661 ccatatgttc aataaatgaa ctgtacaacc gcggatcaac tttgcctaca cgcttgacac
    27721 tactaatcaa cattgtggct tccataactg acctccttta atttatacat gaataataca
    27781 cctcgttgaa agcgttgtca ataatataaa tgttaaactt acttattttt tatcgatttt
    27841 aattaaccaa aatattaata atgattaaaa caaataattt aatataactt ttattaacaa
    27901 atatgttaat atataagaaa aaccttttaa gaggtgatat atatgcaact acaaataaac
    27961 gaagaatcct taccggtata tgaagcatta gctagtaaaa caaggatcaa gatcatccag
    28021 ctactttcca aaaaaaagat gaatgtcaaa gatttggcaa aagaacttgg agtgagcagt
    28081 gctatcacga cgatgcacgt gaaaaagcta gaggaagcaa acatcatcaa aacagaaaaa
    28141 gtaggacaac aaaaaatttc gagccttcgt gtcgataaaa tcgatatcag ttttccagaa
    28201 aaaatattca atgcttttga tacgaaagaa acatcgatac ctattggtca ctatacaaat
    28261 tatgcaattg aaccaacatg cggtttagct acgatccatg attttatcgg gaaagtggat
    28321 gaaccacgct attttatgga tcctcgaagg atggatgccc gcattctctg gtttaccagc
    28381 ggatttgtcg agtatcaggc acctaatttt ttaaatacag aggatacatt ggagatgttg
    28441 gaagtttctg tggaaatttc ttctgaattt cctttttcaa atgacaattg gccttcagat
    28501 atcactttca gcctaaatgg tgtggagcta ggaacatgga cgagccctgg agattttgct
    28561 gatatccgcg gtaaatatac gcctgattgg tatccagata atcttaatca atacggcttg
    28621 ctgaagacga tccgaattac caaacacttg acgaatatga atggagaacc cttgtcaaac
    28681 atcacgatca acgacatccc taaagagcaa gacacttggc atttgcgaat cgaagtgaaa
    28741 gacgatgcca aacatgtggg gggctgtact ttatttggta aaggattcgg taattacgat
    28801 caggatatca aattaaaagt ttactacagc tgatattttt cttttctatt ttataaaaca
    28861 aaaacagcca atccctatga agggacggct gtttttgctt tattgttatt tccagaaatc
    28921 atccaaaacg gaaattggca aatgtcgttt atgttgtgtc ttgttccacc atgcttcgat
    28981 tttttcttgt gcttgagcgg gaacttcttt tccttctaga taatcatcaa tttcattata
    29041 tgtgacacct agtgcgactt catctgcaat cattggtttc ccatcttcaa gatctgcagt
    29101 cggaattttt gtgtataatt tttccggtgc attcaatgct tgaagcaatt gtttcccttg
    29161 tcgtttgtcc aaacggaata atggcaaaat gtcggctcca ccatcaccaa atttcgtgaa
    29221 gaatccagta atgttttctg cagcatgatc tgtccctaat acggctcctg ctctttctcc
    29281 agctacggca tattgcgtaa tcatacgttg acgtgctttg atatttcctt tgttaaaatc
    29341 gctgattccc acacctgctt cgcttaatac ttgtacttgt gcatcaacag ctggtttgat
    29401 atttactcgc agactaacat cgggctggat aaagtcaaga gcagcttttg catcttcttc
    29461 atctgcttgt tcaccataag gaagacgaac agcgataaat tgatagtttt catcttttgt
    29521 ttcttcacgc atttcttcca tcgttagttg cgccaaacgg cctgctaaag tagaatcttg
    29581 gccgccgctg atacctagta caaaagtttt aagaaacgga tattttttca aatatgcttt
    29641 cataaagtca atacttttac gtatttcttc ttttggatca attgttggtt tgacacctaa
    29701 ttcattgata atttctgtct gcaggctcat ttattctcct cctatgcttc tgtatgaagt
    29761 tctgctacgc tttttttcat tcgttccatg atagccattt tatggttcca gcattcagta
    29821 gacagatcga ctgggtattt ttgcggattc aagtctcgtt tatactcttc ccatagtgaa
    29881 tctagatttt cttttgtata ttctttgatt tcgtttagtg caggtaattc gtaaactctt
    29941 tttccttcga taaagatatc ttgtaaaact gggcgagctg taaagtctcg aacagtttta
    30001 ttgataaacg tatgcactgg atggaacatg aagatagctt cttcttctcg agggtcttca
    30061 tcccataatg tcacgtagtc tccttcagat ttcccatcaa aatttcgagt gatgcgccac
    30121 acttgttttt tccctggagt agttactttt tctgcattgc ttgataactt gatcgtatcc
    30181 atcatttttc cttcatcatc ttcgatcgat actagcttga atactgcacc aagggctggc
    30241 tgatcatagg ctgtgatcaa ttttgtccca acaccccata catcaatttt agctttttgc
    30301 atctttaaat tcaaaatcgt tgcttcatcc aagtcattcg aagcatagat ttttgcttcc
    30361 gtaaatccgg ctgcatctaa ttgttcccga acacgcttag atatataggc catatctcca
    30421 ctgtcgatgc gtacgccttg gaagttgatt ttgtcaccta attctttcgc cacgcgaatc
    30481 gcacttggca cacctgattt caacgtatca taagtgtcga ctaagaacac gcagtcttta
    30541 tgggttttag cgtaggccat aaatgcttca taatcatttc cataagattg gactaacgaa
    30601 tgagcatggg tcccgctagc tggtatccca aatatcttcc cagcacgtac attgcttgtt
    30661 gcatctgctc cgccgatata tgccgctcta gttccccaga ctgctgcatc cagttcttgt
    30721 gcccgtctag ttccaaattc caatagtgga tcttctccga tgacagattt gatacgagct
    30781 gcttttgtcg cgatcaacgt ttggaaattg accatattca acagtgctgt ttctactaat
    30841 tggcagtgcg ctaaaggccc ttccacttgg attaatggtt catttgcaaa aactagttct
    30901 ccttcacgag cagaacgaac tgttgcttta aattcaaaat tttgtaaata ctccaaaaaa
    30961 ccttcaggat agacttctaa tccacgcaag taagcaatat cggaatcatt gaacgtcaga
    31021 ttctctaaat aattgactaa ccgttccaat cctgcaaaaa ccgcgtatcc gtggttaaaa
    31081 ggcatttctc gaaagtaaca ttcaaaaaca gcatgtaaat cagctcgacc taattcccag
    31141 tatgtctgca tcatattgat ttgatataga tccgtatgca gcgtcaaact gtcgtctggg
    31201 taagtattat tcatcttgta ttactcctct tggcaagttt tcatttccta cccattataa
    31261 caaaaaaaat taataggaaa agaaatcttc cattttttct gaaaaagcac atgttttacg
    31321 gatatttctt gctaaaaatg aactccatct gctaaaacta tcctttctta ttggtattta
    31381 acctaaacga gaaagaatac ttattttatt caatgtatcc gagacacgaa gaaattagcg
    31441 ttttctattt ttcttacttg ataaaataag tagattttcc tactacaatc gtagccgtta
    31501 agataccgat gattgaacaa atataaccaa atgtctctac aacataatca atggcaaaag
    31561 tttttgaagc aataatacgt ctttcctttt ttgcttctta tccccaaaga aacggatata
    31621 acttattatg tattgaattt ttttggaaag gcgaattatg acaagatata ccttgcgttg
    31681 aaaaaactcg cggaaaaaat atcacacgtt tttaaaaaga tacaaagtaa gaaagcagct
    31741 gtcaatagaa tggaaacaaa aatggcgaga catcaatcag tctcgccttg ttttttattg
    31801 tatacattag atatggagca ggttaccata aagatgatta ttagtcgtta ttaaatagaa
    31861 tccttcgtat ttttttgttc tatcactaca atatctttat ttggacgttt gaattgtatt
    31921 ccggcacgtt ctagctcctg tttcactcct acatggactt tgacatcttc tcgtgtcatt
    31981 tttaaaacgt gatgaatcgc atagtcgatt tcttgccttg tgattttttt cttcgtattg
    32041 atcatcacga cttgcttttt atcatatgtg tctaccaata gcagttgtgt tcgatctaac
    32101 gaaaataatt tgtaataatt aatagccgta ttcccaaaaa agaattccac tactttagga
    32161 taaaatgtct taatatttaa attactattc acaggtgttt cgattagctt catctcattc
    32221 ctcttttcta tatagtcgat ttagatccaa tcttaagtta tgttaccatc tttcctagca
    32281 ttaaggaatc ggttacaatt gaacctttaa agacaaaagg atttaccttt tatcacttaa
    32341 tagtataaca taatttcgtg agaaaaaaat acaatttttt cttgaccatc tcgttacgta
    32401 aaggattatg cttacatttg tcaggaggaa aaataatgga gtacacaata aagaaaatgt
    32461 cagagatatc aggagtcagt ccgagaacct tgcgctttta tgatgagatt ggactgctaa
    32521 aaccagctag gatcaattct tcaggttatc ggatttatgg caaaaaagaa gtcgatcgtc
    32581 tgcaacacat cttattttat cgaacaatgg cattcaagtt ggatgatatc caagaggtct
    32641 tagacaatcc atcttttgac caccagaaag ctttaatcaa gcatcagcaa atgttgctgg
    32701 aaaaacgggc acaaatcgat accctcttga caactgtcca acagacactt gatatgtacg
    32761 aaggaggaag aaaaatgagt gacaaagaaa aatttgaagg gtttaaacaa caaaagttga
    32821 aagaaaatga agaatcctat ggacaagaga ttcgacaaaa atacggaaaa aaaaaggtta
    32881 cagcatccaa tgaaaaatgg atgaatatgg ataaagaaac gtatgaagaa atgcaaactg
    32941 ttgaaaagca gatgttattt gatctcactc tttacttaaa agaaccagaa gatgaaagct
    33001 tagcagaccg tatcttccaa gctcataaga agtggttctc ttacagctgg cctgaatatc
    33061 agccaaaagc gcataaagga ctaggaatga tgtacgaagc cgatgaacgg ttcactgcat
    33121 attatgacga gcgaagtggt catggtgcag caaaagcttt aaatgaaatt atccaaaaaa
    33181 aggcgtaatc ttttacatga tccaacaaaa aagtacacgt aaaatgacct aataacaaaa
    33241 cagcagacac ccaagttaga tttttatgct aacttttggt gtctgctcca ttctttcaca
    33301 agttttttca tcctacttta aatatattca tcgttgaata aacgtataag gtattttcac
    33361 tttatccttt ttttctgtaa gaaacaattg aaatgccatt tctccgcaag cttccagatg
    33421 gtggtcgatg gttgggaaac caagaagctc gctgatcagc aaattatcgc gtccaatcac
    33481 cactggtgtc ttatcttgca gatagctttg caagatgcct gctgcaacca tatccgaatt
    33541 tgtaaagaca ccatcaactc caagctcttt aaaaaagaag cctgcttgtt ttcctttctc
    33601 tgcatctata caatcccaaa aaacaagtga ttcatcaaaa gatggaaaaa gttgatgaca
    33661 tagctgcaaa gtgatttttg agttataact tagcctgcca cttcgcccca acgtgatgcc
    33721 caattgcttt ataccttgct gcttcaaata agtaagacct tctgtaattg attgttctcg
    33781 atcaataaag acacagcttg cctgttttcc cttgatttct tcacaaaaaa ctagaggtcc
    33841 atatttttga tatgccaata aatcatctag tgtattggca cgagacgtaa tgatcaatcc
    33901 atcgaaagct ttagctgcaa attcttcaag ataccttcgc tctttttctt tatgatagcc
    33961 cgtcggtaaa agtgtcactt tgtaatcata atcaaacgct gcttcaatga tcccactgat
    34021 caactgatca aaatacggat gattggtata aggcagaatc actccaatgc ttttcgtttt
    34081 tccataactt aactgtctgg catttgcatt agggatataa tcgacttctt tcatgaccgc
    34141 aagtatttct ttcctttttt cctcatctac atatggatgt ccattgatta cacgtgaaat
    34201 agtagaaact gaataacctg tgagctttgc gatatctcga atgtttgcca ctgctgcttc
    34261 gccacctata caaaaagatt ttcttgtaaa tagtctacca cagctggacc gcattcaaaa
    34321 aactgataca tttttccttc atgcgaaaaa ctgatccgct gcccgcccca atatatatct
    34381 tttgtcaaat tttcgatttc attcattggt atttctttta ctatattcct attcaaccct
    34441 tgaaaactgt acaacgttaa tacggatccc ttgaactcga caaacgcata aaaataattg
    34501 ttcgttgtgc catctgttat ctctacataa gtattcattg caagccctcc ttttgatact
    34561 ttgagtatag tgtatgaaac gcgtctcata gcaaggagtt tgttttgcca aagaaaagtt
    34621 ttattcctct ttcattatta ccaaagtctc ttattttaaa ttgaatacat tataatgata
    34681 ctgtaaatat atctttatta ttagtgaatg
//
LOCUS       DACDYJ010000023        34289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDYJ010000023 DACDYJ010000000
VERSION     DACDYJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.023"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..313)
                     /locus_tag="IWM51_001155"
     CDS             complement(<1..313)
                     /locus_tag="IWM51_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970588.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YE"
     gene            435..1019
                     /locus_tag="IWM51_001156"
     CDS             435..1019
                     /locus_tag="IWM51_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970589.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            1030..1275
                     /locus_tag="IWM51_001157"
     CDS             1030..1275
                     /locus_tag="IWM51_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970590.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            1281..2003
                     /locus_tag="IWM51_001158"
     CDS             1281..2003
                     /locus_tag="IWM51_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970591.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            2134..2877
                     /locus_tag="IWM51_001159"
     CDS             2134..2877
                     /locus_tag="IWM51_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970592.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            2883..3248
                     /locus_tag="IWM51_001160"
     CDS             2883..3248
                     /locus_tag="IWM51_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970593.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            3264..3794
                     /locus_tag="IWM51_001161"
     CDS             3264..3794
                     /locus_tag="IWM51_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970594.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            3956..4264
                     /locus_tag="IWM51_001162"
     CDS             3956..4264
                     /locus_tag="IWM51_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970595.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            4260..4679
                     /locus_tag="IWM51_001163"
                     /pseudo
     CDS             4260..4679
                     /locus_tag="IWM51_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4816..5616
                     /locus_tag="IWM51_001164"
     CDS             4816..5616
                     /locus_tag="IWM51_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970596.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            5653..5835
                     /locus_tag="IWM51_001165"
     CDS             5653..5835
                     /locus_tag="IWM51_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970597.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            5946..6263
                     /locus_tag="IWM51_001166"
     CDS             5946..6263
                     /locus_tag="IWM51_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970598.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            6266..6498
                     /locus_tag="IWM51_001167"
                     /pseudo
     CDS             6266..6498
                     /locus_tag="IWM51_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6613..7092)
                     /locus_tag="IWM51_001168"
     CDS             complement(6613..7092)
                     /locus_tag="IWM51_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970599.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            7212..8549
                     /locus_tag="IWM51_001169"
     CDS             7212..8549
                     /locus_tag="IWM51_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP9970600.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            8572..9099
                     /locus_tag="IWM51_001170"
     CDS             8572..9099
                     /locus_tag="IWM51_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970601.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            9086..9649
                     /locus_tag="IWM51_001171"
     CDS             9086..9649
                     /locus_tag="IWM51_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970602.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            10071..10808
                     /locus_tag="IWM51_001172"
     CDS             10071..10808
                     /locus_tag="IWM51_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970603.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(10825..11241)
                     /locus_tag="IWM51_001173"
     CDS             complement(10825..11241)
                     /locus_tag="IWM51_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970604.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            11633..12880
                     /locus_tag="IWM51_001174"
     CDS             11633..12880
                     /locus_tag="IWM51_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970605.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            13148..15538
                     /locus_tag="IWM51_001175"
     CDS             13148..15538
                     /locus_tag="IWM51_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAP9970606.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            15587..17422
                     /locus_tag="IWM51_001176"
     CDS             15587..17422
                     /locus_tag="IWM51_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9970607.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKSVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            17497..18354
                     /locus_tag="IWM51_001177"
     CDS             17497..18354
                     /locus_tag="IWM51_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9970608.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            18344..19453
                     /locus_tag="IWM51_001178"
     CDS             18344..19453
                     /locus_tag="IWM51_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9970609.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
     gene            19792..20592
                     /locus_tag="IWM51_001179"
     CDS             19792..20592
                     /locus_tag="IWM51_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970610.1"
                     /translation="MVGVSKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            20680..21486
                     /locus_tag="IWM51_001180"
     CDS             20680..21486
                     /locus_tag="IWM51_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970611.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(21508..22935)
                     /locus_tag="IWM51_001181"
     CDS             complement(21508..22935)
                     /locus_tag="IWM51_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HAP9970612.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTYGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGY
                     KTPKFVHFLSHLKLYPILPYTAPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTIT
                     RDGYRTYKSNPKFCVSCPLLENCTLSTKHQKIVTRHIWADLMDELEHQRHTALNREIY
                     KKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKTKKPM
                     AFYFKIMQRLTKRSGLITNSFLSTI"
     gene            23256..24125
                     /locus_tag="IWM51_001182"
     CDS             23256..24125
                     /locus_tag="IWM51_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP9970613.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            24145..24492
                     /locus_tag="IWM51_001183"
     CDS             24145..24492
                     /locus_tag="IWM51_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970614.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            24505..25767
                     /locus_tag="IWM51_001184"
     CDS             24505..25767
                     /locus_tag="IWM51_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970615.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            25847..27223
                     /locus_tag="IWM51_001185"
     CDS             25847..27223
                     /locus_tag="IWM51_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP9970616.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            27220..27849
                     /locus_tag="IWM51_001186"
     CDS             27220..27849
                     /locus_tag="IWM51_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970617.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            27868..28347
                     /locus_tag="IWM51_001187"
     CDS             27868..28347
                     /locus_tag="IWM51_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970618.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            28849..28902
                     /locus_tag="IWM51_001188"
                     /pseudo
     CDS             28849..28902
                     /locus_tag="IWM51_001188"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28906..29505
                     /locus_tag="IWM51_001189"
     CDS             28906..29505
                     /locus_tag="IWM51_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP9970619.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            29521..29997
                     /locus_tag="IWM51_001190"
     CDS             29521..29997
                     /locus_tag="IWM51_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970620.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            30014..30916
                     /locus_tag="IWM51_001191"
     CDS             30014..30916
                     /locus_tag="IWM51_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970621.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            30989..31180
                     /locus_tag="IWM51_001192"
     CDS             30989..31180
                     /locus_tag="IWM51_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970622.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            31344..31931
                     /locus_tag="IWM51_001193"
     CDS             31344..31931
                     /locus_tag="IWM51_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970623.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            31931..32830
                     /locus_tag="IWM51_001194"
     CDS             31931..32830
                     /locus_tag="IWM51_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAP9970624.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            32830..33744
                     /locus_tag="IWM51_001195"
     CDS             32830..33744
                     /locus_tag="IWM51_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970625.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            33796..34011
                     /locus_tag="IWM51_001196"
     CDS             33796..34011
                     /locus_tag="IWM51_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970626.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT        12863 a         4752 c         6794 g         9880 t
ORIGIN      
        1 cctcatattc atagctatca attagctcag ttgaactata agctttttct agtagttcca
       61 tttcccattt tggagagttt ggatcacttt ttcttagtct agctatatct tctaaaattt
      121 tcttagaagt attatcctcg taaatatttg ctaaatcaga taatttatca attaaatcta
      181 aaataagcgc actgtaattg acagctattg cagaacgata atttccctga taaaaactac
      241 ttctaacttc ttctaataat ggaataattc taggattttt aacttttttt atttcatcat
      301 ctaatataaa catataaatc tccttttttt atttgtatga caagtttacc ataaaaaaaa
      361 attaaaaagc gagacatatt tatgttgttg tgttatcata ataacataaa tatgtagagt
      421 gagatgatga aaaaatggat ttgaaacaat cgttttacat ctggttatat cctgataatc
      481 ctatgtcaat aatggctgat atttttgtag ttagcatctt attgtattta ttttatctaa
      541 ttttctttca caatttaatc gggtacatga ttcttgattg gaaaggaatg ttcgatatag
      601 aaagaaaacc atttgctaac aatcaaacct ataccgtctt cgaaagtgga gattgggaag
      661 aaaatgaagt tgatgtgatc catgaaattg gattagcttt atcaggaata caagatgctg
      721 atgttttggt atcaatgaca acggatactg gatacaacgc ttatgaggta ttttatctta
      781 tgtattttat atggcgtgat ggtcaagaaa ttatcaatgg tcgtgctgaa aaaacaatca
      841 attctaatac tgaaatttct ttttgtatgg gtgaagggga aatgattgta tataaagatg
      901 ggtcagcaaa cattaaaaca gcctttggtg atattgaaac aagtattaag gaacttaggg
      961 aatatgtgca gaacccatat aattttcaat atttagcagg aagaataaat ttagtttagg
     1021 agtaaaaata tgaaaaaaat tataaatcaa ccaacaaatc acttgataat tgaaggtgtt
     1081 attttcgtaa caatgtattt tactgtagga aatggcatct caaatacaat cattcaaaaa
     1141 aatccacaat acctattatt tagtctttat gcttggattg tacaaatggt ttgggtttcc
     1201 atattgatgc tgttgtttta ttggatagta cgggttattc ttttattgtt aaacaaaaga
     1261 aaaaaggata ggtgaataaa atgaaatcaa ctaaattgaa ggttgtattg ttatcgacag
     1321 cagcattaat aacccttaca gcatgtggta caactaatag tgctaataat aacaaagaca
     1381 caaagtctag tagtacgatg gttactaaaa acagcactac atcggcatca acaacaatgt
     1441 cttcaatgac tgaaaataat gctgaaaagt atcaaaaata cgaagacatt aataaaggtc
     1501 aaaagccgga tgaagtttat aacaacttta ttaatacagt aaaaaatgat gaaaattatg
     1561 ggaatgatcc attgcaaaat aaaaatctga ctatcgttct ttacaaacct gattgtccag
     1621 attgtcaaaa agcagaatca aaaatcatgc aagaaataaa agaaggattt agttatgctc
     1681 caacattaat gcatgatatt accataaata aaccagctta cgagtattac gcagttgctg
     1741 tagatattac tgatggtgta cctgagtggg tacaaaacat tcctggtctg gctgatgaga
     1801 acaatcaata tcatacgcca acagttgcag tagtaacacc aaagaaagtt aacggcacac
     1861 tatactggga tgtaatggga atgacaacaa atacaactga tgaaatgatt gaaagtacaa
     1921 ctaaatatta tatggatttg ggagcatata aattatatca acaatcagag cctgttatgg
     1981 acgatcaatc caaagttaaa tagatcttta gggcgtaagc cctatttttt taaaattaag
     2041 tgataacaaa taaatgattt ctgaaacaaa cgttcttact caactactcg aaacacacaa
     2101 gctaattaac tagattataa aggagaatgc gtaatgaaag aaatcagtgc aaatgaagct
     2161 catattccgc aaaagaatca ggttggtata tataaggtgc ttcatggtga aggaatttat
     2221 aaaatacagt tacttaccgg ttacaataat ggtgcaaatg ggaagtggtt aaaggcaaca
     2281 agtattcgtg aacttagaag tgaagtagaa aaaattaggc ttggtctcac aagtgaagaa
     2341 atagctaaac tgaagggaaa taattctttt gtaattgaaa aagaaaagga aaaacatcta
     2401 ctttatcgtg agctgaaaat tgaagaatac tatgaaattg atttacctga aaaattaaga
     2461 attattgatc aacgttcaga agaaaaatgg aaaactgaaa tatatccaag cttaaatgac
     2521 tacaagacat caggaaagcc catgtataac tacacaggct ttttaaccta cacacaatac
     2581 aaatatgcct ttcgcaaatt aaaaggcatt aaacaagctg aaattgctct gtatgagatg
     2641 aaaaaagtgg atagattgaa attgattcaa gatttagtgc aaaatacatg gaatcaagcg
     2701 attgtcgagc tagaaaaaga aaatgggaaa attttaacta ataaatatct gatcgtaaat
     2761 gaaattgaag aattaattgg tttattttca tgtgaaaaag gtgaagtctt cttgaagtca
     2821 tcaccaacct attcaatttt ggacagtccg tatcagttta tacaggcaag gagatgagtt
     2881 agatgtctag cttgaaagat gactatatcc aagcaaaaca agagtatgaa aaaatacaga
     2941 tgttgaaatc tgagaaagtc gaattatttt taacacaaaa attagcagag gatcatattg
     3001 ctaacttaga tgctaaaact ttagaggaat atgatcggca gtttaatcag atacatcaca
     3061 atatcatttt gcttgttgag caggcaaagc gaaggtttga aaaagcggaa tatgatctat
     3121 tagatcaagg aattgtatgc ttggataagg gattgcgaag atattatggt gcagagttaa
     3181 aaaagcaaaa tgtagataga aatgcactaa aacatgaaat cttagaattg ataatgcaac
     3241 gaatttgaag ggatagaaag caaatgtcac aaaacattag tgctgtaaaa caattaatag
     3301 aagaagattt agagaccaca gttcagagat tagcggaacg aatccatttt gcaaattatc
     3361 aaaccatctc taactggtca agcaggaaca gaaatccgga agaaattcca atgaaatttt
     3421 tttatcgaat cggaaaagaa tatggtgttg atccgataaa aattttcgag aagaccatga
     3481 tttattacta tcatggaaca cctaacgaag tgttagaaca tcttttggaa gaggcaaaag
     3541 aaaaatcatc aatattagag ctatcgcaaa atgacaaagt tattcaagtg aaatgcgttg
     3601 atccaacagg aaagattatt gtcggttttg atattgaaag aaaatccaaa atagatttta
     3661 ttacacaatt aaaaaagata aataatttag atattctaac gcttagacca gatcatgaac
     3721 agttgtttat gagttttaaa aaacaattga aagaaataca aaaaagatat aacaaggtgt
     3781 ttcctaatgc gtgaaaagct caaagaaatc agagtttttt ggataattaa caaagaatgt
     3841 atcatcttat atactcttgg atttcttagc atgatcttgt tttggtggtt atatttaaaa
     3901 cagtaattgg agagggtcaa gaggattttt atcaagataa ggatgtggaa actgcatgtg
     3961 gaataataga ttaaaaacag gattgttatt aatagttatt tcctgcgcta tgatgattgg
     4021 tatgaggata caaagggaac aatcctattt tgaagtttct gctaacaatg taatagaaag
     4081 atgccattat ggtcaacatt tttggactgg ggaagtcaga gaaaatatag atcgtgaata
     4141 tgttcaaaga attgtttggg atgcatattc tatcaaagat tatcctaaaa gtttaacgag
     4201 tagattattt tacagtgaaa aagataatca aaaactctca gatttgatga tgaaaaaagt
     4261 atgactatta ttacgaaaga gaaacagagt gctatattcg aggggaaaaa gcctacaaca
     4321 aaagtgacta ttggggtgac aaggcgaagc gattaactgc aaattttaga gtgtatgaat
     4381 cagagtttat aaacttgacc tttcttaata gtgtttttat tgattatgta attactacga
     4441 aacgcattgg aagttattca agtacctacg catccttagt tccaatgtta aactcgatga
     4501 gccgtttctt aaaagaaaga gaaaagaaag agctacagta tatcaaagaa atcatgccgg
     4561 attttgaaat gcacgaatgg caaattgctt tatcagattg gaaattggcg aataaggttc
     4621 gtagaataac accttttcaa gccaagcgct ttttgagagc tatgaaattt gtgcaataaa
     4681 aaaagccacc agacaagttg atggagacta acacaattac caacagttgt aattgtgtta
     4741 gcttacaaat taattatggt gtttagtcaa tcccaaattg tgaaatggca ccaataattg
     4801 gaaaggaaaa atgaaatgtc tgaagattta cgagagctga agttacgaaa gaagcagtat
     4861 gatcagtatt tttccggttt gttgaataaa catattgaag aaggacttga tcatgatttt
     4921 ggaaaagatg aatattgttt atattgcgaa cgttacaatg aaaaaatgtc tgaatatcta
     4981 cagctgaaaa aagagataca gaaattagag aaaaaagtac atgcttggaa gtataaagat
     5041 tccatgtctg gttatgttga atctcaaaat aagccaagaa aaaagaagca agcgtatgtt
     5101 ctaaagatgg taaatgggga agagttaatt gtattagcgt acagtgaaga agaggttcga
     5161 gaactcgttg aaacacctgt caggaaaaag gttgaaacaa taaatgagat agacgacagc
     5221 caatacaata gcatctatat tgaaacaagt catggtggat ataagaaatt agcaactttc
     5281 cttagaagtt ctaaagttgg gataatttct aaaaaagaca taagtgctgt taccgatagt
     5341 ttttgtactt tatctgttcc taaagcttat ttccaatacg atagtaaaag atttgaacgc
     5401 ttagagaaat tctgtagacc gtatactgta ttgaaaacta aaaaatttaa taaaactaaa
     5461 agagtttcta tacagttacc gactggtcaa atctcattag gtgaaaaaga ctatctgata
     5521 aaagtaggaa aagagttaat tataaaagta ccggaacact tatttaaaga aaattttgag
     5581 atacgtgaaa ccttggtatg gaaagaagaa aaatagtttg gaagacatag aatggtataa
     5641 ggggaaattg aaatggcagt aaataaaata ttgcaggtac taatcataga cgaatttggc
     5701 ggaggagaat ggtttgatgt gcagtccata gactttgaag aagaagtcgt agttatagaa
     5761 aatatttacg gcgaagtata ttcatattcg cttgaaggtg agaatattga ggatttacgg
     5821 ttgattggtg agtagaaaaa aacagaaata tcaaaatgga gataaaaatt aatagcatgt
     5881 aaagatagtc atgcttgggt taaaaatgtt aaaattcaaa aaaatgagtc agaagggata
     5941 aaagtatgaa aagagaggaa tacaaacaaa gattaaacga actgcttgaa gaggacgaaa
     6001 cattaactca tggttcgcct gatgaaattc tatacatgat tgataatatg gtgatctttg
     6061 gtggatatga acttggtaat aggtcagttg atcataatat tttagaattt gatgatgtat
     6121 catgggaaga aattcttgat tggggaattt tggcggtacc tgaaactaaa acctatatta
     6181 gtgacactat ggttcctttt tttgaggaat tggattataa aagacttccc aaaaatgaaa
     6241 atcatatact gggaggaaac taacaatgtt aagtgactta tttattttac tgatctatct
     6301 aggtgtaagt gaaattattg cgtatttttt gtttgtcatt gctaattatg gtatttccag
     6361 tgcaatcgtt caaatgttta gattaccagt aagtgaacta aaaaaatgat gagattatca
     6421 gcatatcttt taatgttttt gaatgtggtc acgttaatct ttgtagtaat cggtgtgact
     6481 ttcgatttaa tggaatagtg ggaaatctat aatggaaaaa agagacacga atgaacatat
     6541 tttttgaatc aatattgttg atggtttttg cgatcagcgc ttaccagaaa tctaaaaaag
     6601 agtaatgatt gtttaatcat cactcttttt ttgtagggaa attgaaatat gtggagcttg
     6661 tattggttta gttaggattg cttggctata aggagctagt tctttatcaa aaccatcaaa
     6721 gtaggttttc agtagcgatt taactgatcg ctcatcttgt ttgatttgga taatttcagc
     6781 atccaaatcg gttgtgagat caggagcttt tgatgcgatt acctgatttt ttttatcttc
     6841 caactctctt aggctgttta acaagttgta aaaagaatca ttttgattgt tcattccaac
     6901 gtgagttgca attttaacag aagtttcgtc cgtattgtca tcaaaagaac tcattggaat
     6961 atcaagtagt aacgaggatg tttcttttac tgactttttc tttacagcat tttttgtttt
     7021 tccatctttt ttgctcttta ctcgagatgt ttttggagct agtttaggta aatcacttgt
     7081 tttatcagtc atttattagc ttttcctttc tattaattta tgttattata ttatcataat
     7141 aacataaaaa agtacacttt ttgggggcgt agttattgaa aaaatctaag agaaagattg
     7201 ccacattgct tatgttaatg gtgcaattta cagggattgc aatgagtgta atcacaataa
     7261 atccgcaagt gatacatgct gatgggatag ctacttatga tgcacaattt aactgtacta
     7321 ctgaaggtag tgggtcagga acagatagcg atggaaacga tactggaagt ggcgctgtaa
     7381 caggtgatgt agccaaaaat attgaagata tttacaaata catgaatgga gaatatggct
     7441 tatcaggtgc tagtattgca ggtattttag gtaactggaa acaagaatca ggtattgatc
     7501 ctcgagcagt agaagggtat tacggagatg gacagagaga agttgacttc atcaaacaat
     7561 ttactgatgg tctgatgggt attggctatg gtcaatggac agctgggcga catacagcat
     7621 tagttgattg gtctaagaaa aataatggcg gttcttggtg gactacagaa gcccaattgg
     7681 actttatgtt caaaggtgat gggggattcg taggaatatt aaaagattat gctttaaatg
     7741 cttctgatga tgtcgtagaa aatgcagtaa cttttcataa aaactgggaa atttcagctg
     7801 attccgaatc agctgtaata gctgtacgtg gtgaaaatgc aaaagaaatc tggaagtaca
     7861 tgaaaagcaa gggtatggac ggtgcaaaag atgaaagtaa gatcaataaa atatccggag
     7921 gttcaagtac cggagatgga tcgaataatt catcaagttc agcatcagga acagtagaag
     7981 atgcctgtgc aactacggat gacgctaatt ctggttctgg atcaattggg aaagaaggaa
     8041 aaattaacgg taaaaaaggt caagtaatag cgaatatgac ttatgaagaa gctttgaaaa
     8101 aatatgggaa agcgatcacg ctaccaaaat tcaaagaacc agattggtca acttcacctt
     8161 ttaacggaat atatcaaggt cagtgtacag agttgacgtg ggcttatatg agccaattat
     8221 acgatgggaa tcaaccgact actggaaatg gtggttatgt atgggaaagc tacaaatcgg
     8281 caggagcaaa aataacgaac aaaccaatcg taggttatgg gtttagtgct tcggatggat
     8341 ggttatgggc tgttggagat gcagggcata ctggtgtagt tatagcggtg tttgaagatg
     8401 gatcatttct atgtgctaac tttaatgttc caccaaccca agctccaaca aggggagttt
     8461 cggtaacgtt gattgatgga gtggacggat cagataatgt gcatttcttt agcggtgtag
     8521 gaaaagctaa atttgaaatc gaaaaataaa ttggaatgaa ggacgatata gatgaatagt
     8581 aaagtgaaag atacaattat catttctttg ctttctggta tgttattagc actgcttgtt
     8641 ggaatcctat tcttcaataa gcagaatcag gacatagaag ctgaaaaaca cagtgtgcat
     8701 attgaagata agctgacatt ctttaaagag gttaaaccaa atgaggtgga tggattacgg
     8761 aaagatttag aaaatgatat tcaagaaatg ttgaatggat cagttcaaaa taagtttgca
     8821 gatcaatcag gtgaagaatt tttgagaaaa ctatttattg taacaggtaa accaccaatt
     8881 actgataaga gtagtgagaa agaaaaaatc gagtactata agccttttac ttttaagcta
     8941 acaaactttg gaattaagac atctagtagt gggttgtatg aagcattgac cgattttgaa
     9001 gttcaataca atggaaaaaa agtgtatgat accacattaa ccatcacact aaataatgat
     9061 aagaaagtga ctgggggtgc tttttatgca aaatcatgaa atcactactg ctaaaaaggc
     9121 gatcggaata ctttccataa ttgtgatcct ttgttgtgtg gtactatttc aaaagaaaaa
     9181 tgcttatgat aataatgtca aagaattgga agaccttaaa agtaaacaaa tcgaagtagt
     9241 caagaataac cagaaagtga aagctatgca ggaagctcaa tacaaagaat tgggaaatgc
     9301 tgaaattaag gaacaaagta ggaaattctt taatttattt tacaactgga attcatggga
     9361 aaaatatacg aagaatatga aggaaataca aaaagaattt cctcagatag taaagtctag
     9421 tcctgttgat atttctggca cgatggtagg cactggtaat agtccaatat ctagctatac
     9481 tgctgattac tatacaacaa atcaaaaagg taaaatggtt gaattgatta cacagacaag
     9541 aaatactcca acaacagcaa caacaacaat ttggcgtgga gtaacaagca taggagaaaa
     9601 tcaattgttt tatttggagg atttagatag ctatgtaaga gcagagtaaa ataaaaaaag
     9661 agctaacggt accaacgtta gctctttgtg ctgacgatga taacaaaacc taccgaaaaa
     9721 ggattgtttc atcacaaaca caaacagaaa agaattgata aaaaacccga acctcagaat
     9781 ttttattaat caattcattt agattataat aaattgagct gaacattaca atacgtatat
     9841 gttgtaaaac gtaaaaaaag tcaaaagtaa atactttctt aaaaagtcaa ataaaactat
     9901 taaagacaat atatatggtt tatttgacat aagaaaatgg tctagatgat aattgtaata
     9961 tagaattgta gacttaaata aagcataaaa taccttttat gataaggtat agctcttaat
    10021 tttaagcagt tttagatata agattgaaag taaagtgggg gagaataata atggcagatc
    10081 ataaattaga aaagttatta gaagaaacgt ggaatccaaa agaatttagt gaatttttta
    10141 tggagaattt tgaaactgac ttagcagtga ttgttaaaga tgctcttaga gagcaagggt
    10201 atcctgaaac tgcaaactat ataaacatta attttacttt gtatactgaa aataaaggaa
    10261 cttgggattt ttgggctact ttagcaaata aggagttaag tgataaatca gacacaggaa
    10321 ttcgtaactt ttttgaatca aacagagatg attatatgta tgctaacaat caaaataaat
    10381 tgaactttag agtagagttt gatgaaacac ctgaagaatt tatagaaaga caaccgccta
    10441 aagaaaatgt agcaaaggta ttggaagatc gttggaattc agatgaaata gtttcaacaa
    10501 ttagcgaact aggcggacaa tatgaaccgc tagtagaagc ggttagagaa gagctaagac
    10561 tgaataagtt tcctgatgtt caaaatattg atgtttctca aattgaaata aacgtcaaga
    10621 tcacgaataa acttgattat ggtagttggg cagatatagc attagaaaaa tacatctact
    10681 caacgttaaa agagtttatc gaaaatagaa tggatatcat gtatctacag catccacagt
    10741 atttaaactt tggtgttgaa atagctacac ctcttgaaga atggaaaatg gagcaagggc
    10801 ttgactagcc tttgctccat ttttttattt tacaaatgaa tacagttcgt cgtttttacc
    10861 aagaatgatt gtttgcccaa ctttgagatt gtcaggtgat ccttcagcgg tttgtaagga
    10921 gaacgttcca ttgaacttat cggataccgt tataggtagc tttaattctg atccttcctc
    10981 atctttgaga attgctgttt ttcgttcatc aaaataaaca gtctttatta aataattttt
    11041 cgtagtgatc tcagcagatt tgtctttttt tgtagtagta ccagtttctt tagaattaga
    11101 ggtattttct acgactattc catctttggc atttgagctt gtgtttattt gattgaatat
    11161 tttcagcgac aagataacgc taagaatagt caatatagaa acaagtgtaa ttaatactga
    11221 taatttcctt tttttgttca ttttttctta acatcctttt ttgttttgat tagttagata
    11281 ttccttatct aacaagcctt tttaggctat taaatgaaga taaaccccaa aaaatattgc
    11341 aattatgaca gtgcaatggt actattatta tacaataaat tttgcgaaag gggtaggtct
    11401 aaatcgcaaa caaaaagaaa ttgaacctag acaaatacaa cttgtatacc gcaagggaag
    11461 caagtttgat tttgggtaaa ggtagaaatt atgttacaaa cataatcaac tattatccaa
    11521 atcgtttgcc taaaggttcg ttcaagaaag tgggtgacaa aacctatatt ataacggaag
    11581 aaggaataga agcattgcga tcacaaacag aaaataaaaa caaaaagaag gaatgaatat
    11641 gaagagaaat tcgcttggta tggttttatt ggcatcagct gtacttgcgg ggttatcagt
    11701 accagtaacc tcacatgctg atgaatggac tgcaaacaca ccggatcaaa tccaaattac
    11761 aacaggacaa tcaagttatg cgttcaaaaa aggagatact atttggtcaa ctgtagagaa
    11821 gcttaaaagc ttaggttaca agactactgt tgctgagttt gccgaattga acaatgttga
    11881 tttggcaaaa ggagaacaac ggactatccc gattggacga actttcaaca tcagtaaaga
    11941 tacattaaca gttaaggaag cagatggcga agtagttagt caaactgttc aaaaagataa
    12001 acctgatcct attgaaaata cggagcaaga gaacaaaaaa tccgagacta caaaagaatc
    12061 ggaaccaaca gcaaacactt ctgataagac agaaacaagt gcttcaagtg ttgatggagc
    12121 aagttcatct aatacaaata attctacaag tgagaaagaa gcatcacagg gaacaagtga
    12181 aagtagcact acggaagctg atactggaac atcaacaaat aactcacaat caacatcaaa
    12241 tgatgcaaat gaatcaacca gtacatcttc tagtgatcaa tcacaaacag gttcgagtga
    12301 tgaaactgac agttctactt caccaagtaa acccgaacaa ccaactaatg ttgtgctagg
    12361 cgttccattt attagtcaag gtaacacaat gctttgcgaa ggaacatctt tgctagaagc
    12421 gttacattat aaaggtgttg caacaaatca agatttaatg acttttgtta aatcaatgcc
    12481 actatctcct gacaataatc caaataatgg tttttctggt gaatggagac acaatgtgga
    12541 tggaacttat caaggtatga atccggctcc tgtagtttca tggggtgttg ctaatggcgg
    12601 aaatgttgta gatatttctg gttcatcggt acaaggatta aaagatgaga ttgtaaatgg
    12661 aaatcctgta gtagcgtggg taacttatgc ctttgaacct gctcaacata aaccaatgtt
    12721 ttggggcgat gctatttgga atcgacatgt agtagccgtt gatggattta aagacggttt
    12781 ttaccatgtt gtcgatcctg tgtttggggc aagttggatt aatgcaggat catttgaaac
    12841 agcatataac gtatctcaat tagcggtttc ggtacgttaa taaaagatat cgtttatata
    12901 ctaagtagtt atatagtctt tatttgtcct tgcttaacta cttagaaaac ttgttgctca
    12961 tggtgggtag aactaaaagg ggtgatgcta tgaatactag agcgttgaaa cagcttgctt
    13021 tcaacgaaaa aaatgattaa taggtgtggt gattttaact taggaatggg cgcgaaggtc
    13081 attaccacac cgacatatcg tggctcattt tcttcgccta actggtataa agaaggagta
    13141 acgagagatg aagcatgaaa aaacgataat tttaaaatca aaaataattt attgttttgc
    13201 gactttagta atgatgttat ctacgcttat aatgcctttt tcagcaataa gagctgaagc
    13261 agatgtcaca aaaccgacaa gtgcaaaatt tatagaaact ggcgtaaaaa cagacggtta
    13321 tattagggtt aatatgccga atcatccaaa tgaatggatg atttcaagtc aatttaaaga
    13381 ttctcatgga aatattggat attgtatgga ttcagaattg ccatccccaa caggaagtgg
    13441 agccggaagc ttgaagtaca agggagctgg cagtgatgag ttctatcgta tgtttaaagg
    13501 tggttttcca tcaaaaacag caaaggaact gggtgcagga aatgatacag aagcatggta
    13561 tgcaacacaa ttagtttcat gggttttagc cggaaacttt aaagtttcac agattgtatg
    13621 gtcacatcca aaccataccg ccgcagaaac agctcgtgta aaaaaagcat ttgaaaaaat
    13681 ttatgactat gctaagaatg gaaaagatac gcccaacact gagttttcta tcacagcttc
    13741 taagacagct gacgaaggaa aatatcacac tttcacatat aaaacagcat caaataagac
    13801 tggtaatgcg aagctcactt ttacatcagc aaaaccagct ggtatgaaga tttatgatgc
    13861 agatggtaaa gaaattacta acaacactgt gaaattaaat tcaagtttta ctattaaagt
    13921 acctgtgact acaccaagtg gtactttgtc atttaaaggt actgctaatg ttagcacaac
    13981 caatcctttt acttttgatg gacgaggtgt gtatcaagat gccgtagtta tgatcactac
    14041 ttcagaaaca aaggactcta aatctttgag tgctaaatgg acaagagctg ttggtcaggt
    14101 aacagttaag aaagttgatc aagatggaaa tgctttaaaa ggagcggagt ttactctaac
    14161 tgacaataac aatgctaaat ctgttaagac aaccggagat gatgggttag ctacgtttga
    14221 tttgcaacaa ggcttaacat acagcttaga agaaacaaaa gttcctgaag gttatcaagg
    14281 ttcatttaaa aaggatggta ttactgttaa taccgatgga caagaattta gttacactgc
    14341 tacgaacaca attaaaaaag gtaaagtaaa agttaaaaag gttgaccaag atggaaaacc
    14401 attagctaat gctgaattta ccttgacgga tgattcatcc caaacacaag cagaaaaaac
    14461 agatgaaaac ggacttgcaa gcttcgatat tgtagcaaac aagacctaca gccttgaaga
    14521 gaccggagta ccaactggtt atacaggcgt attcaagcaa gaaggaatta ccgttaaaga
    14581 tgatggacaa acttttgaat atacagctac aaacactttg aaaaaaggtt tagtaaaagt
    14641 tcataaagtc gatcaagatg gaaaaccttt ggcaaatgca gagttcactt taaccgatga
    14701 aaaaggtgca acacaagtag aaaaaacaga tgaaaatgga cttgcaagct tcaatattgt
    14761 agcaaacaag acgtatagcc tagaagaaac cggagtacca gtaggttaca caggtgcatt
    14821 taagcaagag ggtatcaccg ttaaagacga tgggcaaact tttgaatata cagctacaaa
    14881 taatttgaaa aaaggtgtag taaaagttca taaagtcgat caagatggaa aacctttagc
    14941 agatgcagaa ttcaccttaa cggatgaaaa aggtgtaaaa gaagttaaaa agacggatgc
    15001 aactggtctg gctacgttca acattattgc gaacaaaact tataacttag aagaaacaaa
    15061 agtaccaaca ggttacacag gcgcattcaa acaagaagga attaccgtca aagatgatgg
    15121 acaagtcttt gaatatactg ctaaaaacac tttgaaaaaa ggtgtgatca aagttcataa
    15181 agtggatgat caagggaaag cattagcaaa cgcagaattc actttgacag acgacaaagg
    15241 tgtaaaagtt gtacaaaaaa cggacagcaa tggcttggca acatttaata ttgttgccaa
    15301 caaaacgtac aaaatcgaag aaacacaaaa ccctaatggt tatacaggga aatggtctaa
    15361 agaaggcatc accgttgctg acgatggaca aacctttgaa tatacagtaa ttaatcacct
    15421 tgtacctgaa acagtaagaa caggcggaat cggtacatca gccttccttc ttgtatttct
    15481 aattgcagga ttgagtatgg tgctactgta ttatcgagaa cagcacaaag caaaataaaa
    15541 aagtgttaat ggaaaagaag taaatatgaa aaaagaaggc tgacgtatga aatcaaaaaa
    15601 aattttttca aatttaatgc tatttggtac tttgctagga gtgggattgg gcgcaagctc
    15661 aatctcttct agccttggac taggggggca agtagcatat gcacaacaaa actataatta
    15721 tcaaggaact ggaacgattc atatccataa agtaacaaat actaatgggg atgggaaccc
    15781 tgtctcagat attgataaca aagggaaaga aatagcttta ccaaatggtg taaaacctct
    15841 tgaaggcgtt gagttctctt atgtgaaact tactaaagat gaagctaaat ccttgggatg
    15901 gacggatagt gatactacaa aaggtcaaca tattgatgat gctaaagcac gattattact
    15961 aaacaaaaaa tacagtgaag taagtagaat gaagactgat tcacaaggga atatagacat
    16021 acctatacaa gtaaatacag acgatttaac agataacttg tatttgattg ttgaaacagg
    16081 aacagtcaaa ggtgtagttg aagaagcgta tcctatgatt gttaacgtac ctgaactaaa
    16141 cggatcagat gatcaatact ttaattatga tgtacatgtc tatcctaaaa actatttcca
    16201 attaaactca gcagagcttg ataaaaaaga cgaagatgga aaggctcttg ccggaactgt
    16261 ttttgaattg cgaaaagatg atggatctga tcaggttgtt ggaacttata aaacagacag
    16321 cacaggtaaa ttaactgtag atggtttgga attaggtgac tattatttcc tagaaaccga
    16381 tttgggaaca gatgctaata aaacaaccta tttactagat ggtaaaaaat tcaaatttag
    16441 cgttaaacgt ggggagactg ctaaagtagt tcttgatgtt accaactaca aacgacctgt
    16501 aattactaag gtaattgata aagaaaccgt tgatactggc gaagatgttc aatatacatt
    16561 gaaaccatca attcctggta atattggtaa ttacgaaaaa tatgttgtca aagatgttct
    16621 aggtgcagag ttaacttatg attcatcaga agtgatcgaa ggaatgactt tagatactga
    16681 ttacagtatc gaacgtgacg gacaaacgac aaactatgtc tttactgaat caggtatgga
    16741 taagcttcag aaacaatttg atgcagggga tcgcttcttt acaattttga tgaacgctca
    16801 tactaatagt aaagctaaaa tggatacacc acttaagaat gatgtttcaa cgatctacaa
    16861 caatggatat gaacccgaag acgaaacacc agttgatcac gcttatacaa tcattggtga
    16921 tcaccaattc attaaaacag atggagatac aagcaaagca ttgagtggag caaaatttgt
    16981 agtgaagaac aaaattgatc cgaataaagt tacttaccta aaacaagatc caacaacgaa
    17041 atctgttact tggattgcag ataaatctaa agctactgaa ttcgtttccg gatcagatgg
    17101 taagttccaa attgaaggct tgcaatatac ctatgatgta gattttgata aacaaggtaa
    17161 tccaacaatc acagataaaa aaatcaatga ttatgctctt gaagaagttg ttgctccttc
    17221 aggatataca ccaactagaa atgatgttcc atttgtggta tcgaatgatg atgcttctag
    17281 cgaagtaaca gacatcaaga accttaaaac acctgaaaca gttcgtacag gtggaattgg
    17341 aacagtagta tttctagtgg ttgcaggtgt ggcaggaaca gggctatatg ttgtctacaa
    17401 acgagaacaa cacaaagctt aatgaaataa aattccctag agtgtttttg gctctagggt
    17461 ttctttttaa aaagtgtgtg aaaagtaggt atgagcatga aaaagaagaa tatcaagttg
    17521 atcatcttaa aaattgggat gttgctatgt ttaggagtag gtctatatcc tgtaattact
    17581 caggcttata cctactacca aactcaacaa acactaaact cctataagga aaaatatgat
    17641 gaagcttctg aaaaggagaa tgaagaagtc ttcaagagat attcagaagt taataggaaa
    17701 cgaaaagaca cagaacaaac ctcagtcacg attcctgaag ataataacaa tattccgtca
    17761 aatggaaatc ttcagaagga taaattaggt gaattagtgg gtgaaattct tataccaaag
    17821 attaatgtgg atttgccaat ttatgatggt gtaaacgatt tccaactatc agcaggagca
    17881 ggaatattaa ccggaactga ttatccaact ggtgggattg gaaaccattc tgttatatca
    17941 gctcattcag gcattcctaa taaaaaatat tttacagatt tagagttgct caaacttggt
    18001 gataaatttt ttattgatat ttcagggaaa aaacttgctt atcaagtaga tcggataaaa
    18061 acaattctac caaccgaatt tgaggatttg cttcctgtgg caggaaaaga ttatgtaaca
    18121 cttatgacat gtacacctgt tggagataat tctcataggt tactcgttag aggggttagc
    18181 ataccttacc aaaaaagtat tgttgagaaa gaaaagaaga aagaagattt aaaggtaaag
    18241 gcgcagatga actggtatcc tatcgtagtt gtaggggttt tattaataat agctgtgata
    18301 gtcattatta aaaaaatagt agcaaagaga cgaggaatga aaaatgaaga ataaaatact
    18361 actttttttg actcttattt gcactttggt tttaacaatt tatcctgcaa atcagctcta
    18421 tcatcaattc aaaacaaaag agaatgtggc ggaaatccaa gagaaaattg agaaaaaaca
    18481 aactccttca aaagaggaag tggcaaaaaa agtcgaacaa ataaaatcga acagcggtaa
    18541 ccaaggatta gacaatactc agaaaacaga taaaggagta gacaatactc aggaaacaga
    18601 cggtggatca tttgatattc gtgaagctga aggaaaggtc aatccataca caggagatgt
    18661 tttttcaaaa aatcaaacga aagattttga accatctacc tattcaggag cggttggtta
    18721 tgtctatatt ccaaaaataa atgtgctaga gcttttgttt ctaggcgctt cacaggagca
    18781 tttatataga ggtgtggcac aaatgagtgc tactagtcta cctttatcag gtaaaggtca
    18841 tcaaacagta atagcaggtc atcgtggcgg ttatacgtca ccacaatttc tttatgttga
    18901 tcaattagta gccggagacc acatttatat agattatctt ggtgaaaaag cagtttatac
    18961 ggttttccga acagacgtta ttagaaatta cggtcagaat tcaacactga ctgatattcc
    19021 tgatttagat atgttaacac tttatacttg tcatccatat cctcagaact acgatcgttt
    19081 attagttaga agtctaagag atgaaaaagc aaaatacatc acgccacaag taacagttgg
    19141 caataccgca tacgctgatg ccattgatag cgagtccgtt tcagataaga cagaaactgt
    19201 tacatcttct aatgatttat ttgaagatgt tgatcgtgcc aacaaggaat tagatggaaa
    19261 taatgatctt aatattccgg aacaaggtag cgtagagatg caagacagaa cgtttgctga
    19321 aaaggcaagt tcaatattta caggaattac caataaacat cagaaaatgc ttttagtcat
    19381 ttggactgta ataggagcat tagcaagtct aaaggttctt attcttttaa tcaaagagtt
    19441 tcgaaaagca taatgaagat tataaaaatt gatagagtac agtaaaaagg gattgatact
    19501 attattgatc tcttttttat tttgaataga gctagattca cttttaaata aaatcaaaac
    19561 tgatcttaat tctaaagaaa aagtcaagaa aaaaatacaa taaatttttt tatcagaata
    19621 tttatcagaa tattattaga aaagataaac tcaaaagctt aatttaatag ggttattggt
    19681 tgtatttaga aataatgtat ggaaaagcta taaagcaata gataagatgt agaaaatctt
    19741 atttgtgaat gaaaaacaag tcatcgtaaa atattaatag gaggtgtagt aatggtggga
    19801 gtatcaaaaa cagctttaga ctcattagct aaactattta gtagcaggtt tgatggtgca
    19861 gaatttgttg aatatctaag ccaaaacgaa agtggaaaaa ctctgatcga aacaatgaaa
    19921 aaagactatt tgaatgaaaa tgactttccg acatggacga atattgaaga tgctacatgg
    19981 gatgtagtat gtacgatata ttctccttgg gtaacgattg ctgattttgt tttagataaa
    20041 attggagggg atacataccc tgatgccaaa tctcttgcta caagtgactt taaaacgttc
    20101 tatggttatc taataggtct aataggcaat ggaatagatt tgaaagttac gccaaaacaa
    20161 acacctgaag aaattgaaaa agaaaaagga gaggttcaaa tggcttatgc aaatgacaga
    20221 gaaaaagtaa aattattgga aggtaaatta tctgaagaag aacttggaaa aatggtgcaa
    20281 gaaatcaatg aagacagcaa ctattttcct gaattgaagg tatatcaaaa tgacgaagat
    20341 ttctttaatg aaatgtttag taatagtcca tgggaagcag taagtaaaac aaatttagga
    20401 gaatggaact ttcaagatga tctagttgca tttagcaatg atgggtattt acgatcatat
    20461 tcagaaagtg agtatagagc tgaattaaaa acgtatgaaa cggatattct gatgagctat
    20521 ttaaatctat tagagagtgg tcagggagag ttaagagata aagttgaaga actattaaaa
    20581 gaagagctat agggattatt tcaacataaa cacaagcaga aaactaccaa acaaaacgta
    20641 ccatgcaaaa agtcggttaa accggctttt tgctctatta tgagaggaga tcaaaccatg
    20701 agtgcaacaa ccgatatggc tttagaagca gtaataaaag ataatacagg aaatgtcgaa
    20761 aatgtagaag actttagcga tattgcaatc aatctttcag catggatttc agctgtagga
    20821 gaagaaaata tcgaatttga gatcaagaat atggatgact taaaagcaaa atttcctttt
    20881 attgcatcga aactaagtca agttgccgag gataaatcgt ggaatgaatt agttagtttt
    20941 tccgaaaaaa tttatgatat tacgcaagaa gatgagtgtg tttcatggga aatgaataat
    21001 caatacaaga aaattgacgc tttcgtcaaa tcacgtatag aaggaacagc ttttgagttt
    21061 gaaggtatag ataccgcaga taattggtta aataaagaat cctttattaa aagtatagcg
    21121 gatgatcttg aaaaacaatg gtctaaggaa gactatgcag agtacctaaa aacagaaaga
    21181 gatacagaag tgaaagaaaa actcactgag gtatgtttgg atgaacatat tgatcccaat
    21241 atcgtaaaac aagaagacta tgaagtggtt attgataaag ttcctactga ttttgaggaa
    21301 gttgtaaagt atatagttga agaagcatta tcgcaatact cttcagatga ggaagtggac
    21361 gaagaaactg ttcgtaccag agtttggcaa ctggtttttg atgacaaaga ttataaatat
    21421 ttgagtgatc aaaactcggt taattggagt gtacgattat taattgatca aaatgatatt
    21481 gaataaaata gagctgaaaa gctctattca gattgtagac aaaaaggagt tggtaattaa
    21541 gccactcctt tttgttagtc tttgcataat tttgaaataa aaagccatag gcttttttgt
    21601 tttccatagc catgtggcta gtttttttaa attcatggca gcaaacgtaa gcatcgtgtg
    21661 cgtggcgact ttttcaagac ctcgatattt cgtccaacgc ataccatgct tttcttttgc
    21721 atctgcaaat attcgttcaa tggtttgttt tctctttttg taaatttctc tattcaacgc
    21781 cgtatggcgt tggtgttcaa gttcatccat aagatcagcc caaatatgtc gagtcactat
    21841 tttttgatgt tttgtactca aagtacaatt ttcaagtaat ggacaactca cacagaattt
    21901 tggatttgat ttataggttc gataaccatc acgagtgata gttgaaaata acaacggttg
    21961 attattggga cataaatagc agtcaaaata ggcatcataa acaaagtctt ttttacgaaa
    22021 cattcctttc actccatgag gtgcagtata tggaagaatc gggtagagtt tcaagtgtga
    22081 cagaaaatga acaaatttag gcgtcttata tcctgcatca gcaaccacac gctttatatt
    22141 tggaaaagct cttttcgact gtttaaccag atcgattgcg acttggctgt catgcacatt
    22201 tcctggtgtg attaatgtag ataagataaa accgttatct tcacaggaag tatgtgcaga
    22261 ataagcaaat tgtttttctc gttcaccttt aacatagtaa ccactttctg gatctgaatg
    22321 acttattttt tgaagactca actcactttc tgtgtcccat gtaaaaggac gttttcctac
    22381 agcaatacgt tctttattta tttcatcttc taattcatct tgataagctt ttgcttgacc
    22441 gtaggtcatc tctttcgtga acttatgttt attggcattt gctttgatat gagtagaatc
    22501 aatataaagg ttgtcttccg taacgaagcc agcttttaca gcttgttcta gaatatgaga
    22561 gaaaatatct tcaaataaag ttgtttctct aaatcttcga acatagtttt taccaaaagt
    22621 agagaagtgt gggataggat cctcgaatga ataacctaaa aaccaacggt aagcaacatt
    22681 tgtttcaacg tctttaattg tctgtctcat cgaacggata ccaaataaat attggataaa
    22741 tactaattta attaagacca ctggatcaat actgggacga ccataggttg aatacgtaga
    22801 ttctacaatg ggataaatga aatcaaactg aatcgcagca tcaatttttc gaactaagtg
    22861 atctttaggt acaagatcat caattgttaa catagcaaat tgatttctac cttcaatcga
    22921 ttgttttctc atcattagaa tcactcccag aatagtttat acaattagta taaaacaaaa
    22981 aactgcaaac aacactccaa atttaggagt ttgtctacag tctgaataga gctgaaaagc
    23041 tctatttttt ttgataaaaa aactagctat tttattaaaa ataatttggt tcaaaattta
    23101 tttttttaac ttgataattt tttgccatcc ttttttaaat tggctatata tcaaggttta
    23161 tagtgggaat aaataaaaat acatttgaca aaatttacaa tatagtttat tatatattta
    23221 tcttattatg ataacacaat aaaaggaatg gtggtatgaa aaagaagaag tttagccgat
    23281 cgttgttagg aattttggtt ttagttgcgg ttggtcttag tggcaagggt tactttcaga
    23341 ataacagtac tcaggaacat gattttcaga tcaaaagtag tgttcaggta ggtaataaag
    23401 cagacagtga atcaaaagaa tttttaggga tacgttatga tccaaacaaa tatccaaata
    23461 atatcgtaaa cattaatgat gggaaatata cactgacaaa agaagaccat gaagcattat
    23521 cagaaaaagg cagtcaaacc ttttgggtaa attatgcaga tttagatcaa cttggaagag
    23581 caggagatgt tgaagcgttg atcacctatg atgctgtaaa ttctcactca tctaaaaatg
    23641 tggaacgtcc atctttcaaa agttctgtcc atattgcagg agaatacaag gatggagtat
    23701 ttgatcaaga gagagatact tgggtcggca aagattcaaa caatgaaatc actcagctag
    23761 aaggctataa aggttatctc tataacaaaa gtcattcatt agcatggtcg ctaggaggag
    23821 atatggaggt acacaattta acacttggaa cgagagcgca aaatgttggg aaaaatgatc
    23881 agaatggagg gatggcttac tcagaaacga ttgtacgtga tgcaatctat aaaaataaga
    23941 atgaaaaagt attgtataaa gttgatccaa tttataaagg gaatgaatta gttcctcgtg
    24001 gttctcatgt cgaagctttc tcagttaacg ataatggaaa aacggtaaac cttaatgttt
    24061 atgtatttaa caatcaaaaa ggaatcaaca tcaactatca aaatggtact tgggtaagag
    24121 attagaaatt gagaaggtga agaattggaa acaataagca tcaatggatt agataaatca
    24181 gttgcaaatc gaataaaaaa aggaaatggc tactatcttc tgaagaaggg taaatattca
    24241 gttgctagag agttagaaac aatgagtatc gtgtatgtag ctaagaatca ttttgacgta
    24301 gaacaggcta tttttgatcc agattctgaa atagggaaat gtgttgaatt atcagagaaa
    24361 actgttagta caaaaggggc gatgatcatt gagagagaaa tgttagacga aatcagagta
    24421 atcattccta aagatacgca gatgtgtgag gacagcataa gtgacgatga accaaaatca
    24481 gaaagaaaat aaggagatat tcagatgagc gaaaaatcca ttgaattaaa ggaatataag
    24541 cgaagcgatg gagaaaaata ttatcctgta gccagttttt ctaaatcagt atatctggtt
    24601 ggacgtgcat tggtagttgg tgcaattggc atatatattt tgcattgggt aacaaaggtt
    24661 gtacaactgc ttgtagatac cttagtaagt attcttgtct cgttaaatga tgttaacgga
    24721 acagatgatt taggattaaa agagatgttt aaagagttac tcaatgcgtt ttttcccaat
    24781 tatttaagtt attcatataa tgtacaaagc ttttttgcaa agctacactt ggtagacatc
    24841 tctattttag tattagaagc aggaacggta ttctttgttg gatatttggt tactttgatg
    24901 ttcaggcatc actatggaga acaaagacca ttttttgatg atatggaaag tagaaggttg
    24961 aaaagagaag ctctagaagc tatggatgtg aagtatgatc caaacaagat tataggagat
    25021 gaaacgaaca acaaaaaaag aaaacgaaac aaaacgcaat tagaatccga tgtgctatca
    25081 acgcttaaaa atatcaaaat ttctgttcat acaagacgtg atctaagtgt caatgaagat
    25141 attcgagaat atatcgtaaa aatcccgcaa ccaaaaggga agaaagttag agaaacagtt
    25201 ttacgacaaa ttcaagattt agggtcgatc ctgacaagag caacaagcgg acgagccaaa
    25261 tttggtgaaa aagaagatga agtttcaggc gattatttca ttttccgagc atcagtaaat
    25321 attgaaagag aacgtaagga aagagtaagt accaaaaaca aagaaacgga aagagcaaca
    25381 tcttcaatcg cagtttcaaa ttcacaattt tcttttccaa tcaatctctt tgccgataat
    25441 tccaaaaata tccaaaacca aaccgaaaaa gctagaaaat atgccgaaac tcaaaagcaa
    25501 tctttagaac tttatctatc ttctgctgac gtacctgcaa gtctaacaga tatgcacgtt
    25561 gggaatacat ctattgaata tttgtttact ttatctcgta ccagaaaaat tcaactaagc
    25621 tcaatgaaag aagaaattga aacttacttg catatatcag gtgtaatcgt gcggatcaac
    25681 gcagggcgta ttcaaattac tttaccatta ggagaaaatg atcgtattcc aattgatgta
    25741 ccatcaatga tcaatagcgt gttttagaag acatcacaaa tagaaacaga aaatacaaac
    25801 taccagttca caaacagaaa agtaccgaga aaagtgagga atagaaatgg ctatgaaatt
    25861 attagagaaa gtcaaaaaga gagccgaaaa agctgaatct attttgctag ataaagaacc
    25921 taatataaag cttaatggaa aattcagtgt caatatgttg aacacaaagg ctaaagaccc
    25981 aacagatgct atttttggaa ttaagcctga tggcacaatt gctcattttt gcatcagcga
    26041 aatgccacac atgttggttg caggaacgac aggatcaggg aaatcagtat tactgaatga
    26101 aattcttgtt acagtaatgt gtcactcaac gcctgatgaa ttaaaaatag gaattgtaga
    26161 cccaaaaagg gttgaatttg gaagatacaa aaaattgccg ttcatgttgg ctgatccaat
    26221 tacagatatg gatgaagctt atgatttttt tgaatatctt gtgatcttga tgcatgaacg
    26281 ttacaaactg atggaaaaag cagggttaca aaacatcacg ctatataacg aatatgccga
    26341 aaaaaatgga ttagatagat ttccctatgt gatcttatta gttgatgaat attcacagtt
    26401 ggttggaaca cataaagaag tagaaggttt gattgttcaa ttaggacaaa tggcaagggc
    26461 agcaggtatt catgttatct tagcaacaca atctcctcga tcaacagttg tcactggtat
    26521 tatcaaagcc aattttccgt cacgtgtttg ccttatggta gcaagtgatt tagaaagtag
    26581 gattgtatta gatgaaggcg gtggagaaag tctaagtcca aagggagata tgcttattaa
    26641 gcttgttgat ggaagtatgg ttagagcgca gggagcttac atcagtaaca aagaaattga
    26701 aacaatcttc aatcacctta gaaatactat gccagaacca gaatatgttg attaccgagc
    26761 aatcgtagct gaaactcgtg gtgagggcgg tgaagacgag gattttggtg gaattggtaa
    26821 agctcctgta agaaaatctg ctaaaagaaa tttgaacaca cctttagcaa atccatcatc
    26881 taaagagcca tcaattgaaa agaagagtgc gtttgctatt aagcttgaaa agaacaaaga
    26941 agaaaaagtc gagcagggag ctaaaacact agaaaaactg caagcagaaa aactagaaaa
    27001 agttttaagg gcattaggaa aagctgatcc tgaaccgcaa acctcaaaca acacgaataa
    27061 taaacagccg attgcatcta aaaccaaacc taaagctaat gatccaaaag gctcagaaaa
    27121 cgtagtgatc acaactgata aatctgaaaa gaaacaaata aatggaagta cttcacctat
    27181 catgcaaaaa atgttaaaag caaaaaaaca ggtgcataaa tgatcatcgt cagttctttt
    27241 ttaagatggc ttactccata taaatataca gatgaagtag ttgtgaaacg agcgaagaaa
    27301 agagataaaa aagcggtact aactcctaaa cagatgcttg atcatggcat tttaggggtg
    27361 aaggttttag aagtgaataa accgtttgaa tcaaatagtg caagagaaaa gttgcttgga
    27421 gctgaagccg atgtcgcata tttttaccat gacaaaagca agcatcaaat agctattaaa
    27481 ttcaatccaa aatcggcatg gcggtatcaa gcaagagatg acttgaaacg taggaaactt
    27541 agaccaatgc tctatccaga aggaaaagaa gcatttgata gaacccacat cgttcccatt
    27601 ggttttcatg gtacagaatc agataaccgc ctattagtag gttggaacag caatcaaaat
    27661 agagttcaga tcaataaatt tgagaagaaa gtaaggaaat tcaacgatcg aaaaacgatc
    27721 ctatggtttg cagatatttg taaacagccg gatggaacag caaagtggga agcggtgata
    27781 tttgacgaaa atggtaaaaa agtaatgtct gaaacatggc atgataaagc taaatttgtt
    27841 tggcagtagc agtagaggtg aaaaagattg ctagaacttt ttgaagcaaa tagaacaggc
    27901 tttaatcaaa agttagaagt gggagaaact atagaatata aagataaaaa ctatattatt
    27961 atcggaatct ataacacgag aattaacaga ataggtgatc cacgtataac cagtgacttg
    28021 gtttgtcaat ctgttaacta ttcacctgat ctgacaagca ggtataagaa atacgtgtta
    28081 ttagaatata gacacaagat tactgatgaa attggtgtaa acaacacacg aaacatattt
    28141 aagatcggaa cagtgattcc ttatagtaac aacgaagaaa aaatatttta tcagatttac
    28201 ggaatagaaa agtttgaata tgaacatgtc gatctgctag tgacgtacca aatgcgttta
    28261 atagaacctt ggtcacaggc agaaattgat aaagcagtaa aattgaaccg attatcaaaa
    28321 tttaacgtat taggaaatgc cttttaaaaa tcgcaataca caaacagaaa aataccaaaa
    28381 aattagcaat gaacgtaggt aagaacatga caataaataa taataaccct aaaaaatata
    28441 atgctcttgt ccaatgctat atcgaaaaca aaattacaat gcaagaagtc gctgatgaat
    28501 taggcaaatc aaagagccta gtatcacacg attttaagca caatgttttt ccgaacccga
    28561 ttgagctgag gaaaaaattt caaaagagag tttatcaaga aataggtaag tggattgatg
    28621 aagggaatgc aattgaccct ttaattgaaa aatatccttt tttaaaaagc aaaaataaaa
    28681 ttcaaaggtt aattcgtcaa ggcgatattc aagctaaaga acctgttttg acaaccaatg
    28741 atttggctaa aataaatctt cataaacaaa ttattgaaga ctttgaaaaa aatctttcaa
    28801 caactgaaat tttaaaaaaa tacaatattc cgagaggaaa gctaacatcg actcttttta
    28861 gagaatatgg agaatcggca gttactaaag cgaaggaagt agcgaatgag agaaaaaaac
    28921 aaaaatagta gtcgatacct agtagcgact gtagtttttg tttgtgtatt gattttcctg
    28981 tttgattttt tgcagattgg aaaaagcata attggaaata cattgagtgc aaaggaaatg
    29041 gttgctttag gtggtttgta tgaaggacac ttgttcccga atatattcat ttctatcgtt
    29101 tctcatatta acttgtacca tttaatttct aatttattgt tcttagtagt tcttgaagaa
    29161 gcactgaatg atcagtatga gtggttaaca ttattgatcg ttttaatcat atcaggattt
    29221 ctaggaagct tagttccttt tctgctacac agcaacatcg taagtatggg gcttagtgga
    29281 ggtatttttg gatggattgc tttatttgcg attgcataca tccgttttga tagcaagaaa
    29341 catttattac taagtgtttt tgtattattg ataatcggca ttttattggt atctactttt
    29401 gaatcaggcg aggcaaatgt cttgtcacat gtaatcggat ttgtctgtgg aatgatcttg
    29461 ggatttattc taaagaacgt ttcattgatc aaacaaaaga attaaaaggg agtgctaaaa
    29521 atgccaaaaa gaaaagaaaa gaaaattcca gtgtttttgt ctgtctcaga aatgttatca
    29581 cttcaaatac tgttaagaaa aaagattgaa acaatagacg aagaagttag aaataaaagg
    29641 gctatgtacg aacaacgtga agcgctaaga gaacgaatcc actattttga tgatatacac
    29701 actaaaatct atgaagcacg tacaaatcac gaagacgaaa gtaaattagg attagctcaa
    29761 caacggatac ttgaatggct taatacgaat actgatcaag aaccgttaag agtgattagt
    29821 aatctttttg aatttggaac gaagcattgg tttaatgctg tagagaagga tgctgactta
    29881 caagaagcgt ttgatacgct aagtaaggat agcaaatcca tgcgagattt attgattact
    29941 tatctaaatg agcatgagtt tgaacaaacg aaaaccaaaa agaaaaataa agaatagaaa
    30001 gttgtgatga atcatgctat taaccctgtt aggttattta tgtttaattc ttgctttttt
    30061 tggggcaatc gctataatta ttggtcaatt tcaaacaaat aatccgatta tgcgtaacat
    30121 aggcattgga attgtgattg taacctttat tgtgtttaat gccactaaga tttttggaag
    30181 taacgataaa caatttggta tatcgggagt taataatagt tatgctccag attctttggg
    30241 aagggtagta atagatggaa catttgaaaa tgtaacgagt tataaaaatg tttcgctgac
    30301 agtgaaggaa gatggcaaga aagcatcaga cgacgttctc gctactatta agccgtctca
    30361 atcacaattt gaagtgtttt atacagttaa agagaatagc cctgtacaca agctgacatt
    30421 ggtttttaac gttgatggag aagaatacta taaagatgta aaaataaata aaacatcaac
    30481 gaaaaagatt atcgaatcag cgaaaaaaga agtagctaca gaagaaagta caactgaatc
    30541 aagttctact gtcgaagcgg aactaccaga taaaattgat tttgatcctg aaaaagtcaa
    30601 atcgtttgaa tctgtttctg gttctatggc tgatcggttt aacactgaag tcttcaaagc
    30661 tatcaaaact gttaaattgg atactgaaaa gcaaatgatt aatgttacct tattcaattc
    30721 agttgaatct atggatcagg ataaaaaagt tacggttgca agagaactag gaaaaagagt
    30781 aattggtttg tatcgtggta ctgctaaacc agattcagac ggtgcagatt tgccagttca
    30841 tgtagtttat gaagacggta aagtttttgg agattctaca gcaaacgaag cattaacgat
    30901 taaattggct aaatagccag aaagttataa taaacaaatg gtgtcacacc aataatatat
    30961 aggtgtgaca ccttggagat gatagctgat ggtagtaaat aaagaaaaag ttagaaagta
    31021 tgtgacttta aaaatagctt ctgttaaata tttagaaaag cgagctaaag aagaagaaag
    31081 agcaagaggt aagaaagtaa cagtttcaga gttgttagaa gaattgatcg agaatgatca
    31141 aacaataaga cgtgcattta ctaataggtc tatcagataa caatataatt aataaaaaag
    31201 aaagagtgat ttggtcttag actaaatcac tctttttata taaagtttct ttacactcat
    31261 tccgaataat gttaaaattc tttatatgat atatagttcc ttgtcagagg aagagataac
    31321 cagcaatatt ggagggataa taagtggaaa acaataatga tattagacat tttagcattg
    31381 gggaaagagt tacaacagta agtgaattat caaaggatta tagagataaa gagggaacga
    31441 ttatttctct taataagaat cgaagaggcg tttggcaata tgttgttgat ttaggtgatg
    31501 gcgttacagt tgagtgtaaa gatagtcaac tgatcagaaa taatggcagt cattccattg
    31561 aaaaagcaat tcttgtatac ccaaaagtat caagtaagat catgaatgac tggttgcttg
    31621 gaaaatttgg tgagtacaat aaaaaagaaa tcgcagaagt gatcggcaaa tcttatccaa
    31681 cagtattagg gtatctaaaa aagataacga gtaaagaagc aaaaattgag gatgttttaa
    31741 cagtaagaga gcttcaatca ttaatcgagt atcggaataa acaaataaca aatcctgagt
    31801 accttagaaa agaagatggt ctattgaaac aattggaaca agtaaataaa atccggaaga
    31861 tgcaaaaggt agctccttta agccttgata cctttaaaaa aatctatttg aaagagatca
    31921 aaaaagacta atgtggactg taactttacc gttccaacct gtatctgcac caagaccaag
    31981 tgcaagaagg aatgctgctg atgaaactaa tgacgtagca gagaaaagga tttcaaccta
    32041 ctatgataaa aagtatcttg attatttgaa aaaaataaag agctttatcg aagatcaaga
    32101 tttattagat gatgaatttt actcgattgt aaatgatcct atgggcgcaa ttatggaagt
    32161 ggacttttac taccagatac caaaaagcta taaaaaagtc tataacatta tgaagacaac
    32221 agcgccagat ttagataatt tagtaaaaag tgctatggat ggtattttta acatctcagc
    32281 tatacgagat agccatatca caggcttaat tgcttttaaa tataatgtgg cgaacgaagg
    32341 aaaaacagtt gtgaggatca aacgatattc agaatttgag tgggatactt ctgaaggatt
    32401 gaacggagat atatgggaag ctacatttga ttttaaacca gtagcaacgc caagaccaaa
    32461 gtctaaattt agaggaaatg ggattatcac ttactatcca aaagagtaca acgactattt
    32521 ggaaaatatc aagaatacgc ttaaagagaa agatttggta aatgatcaac tgaaaaaggt
    32581 aatgaatgct cctatggggg ttatagctca gatagattat ttttaccaag tgagaaaaga
    32641 taagaaaaag cttgattcac tgatgcgtac ttcacagccg gatattgata atttagtcaa
    32701 aggaacattg gacggaattt ttaacaagga aataggcatc aaagatagca gggttgtagg
    32761 gttaattgct tttaaataca atacttttga aaagtcgaaa acaaacgtga gactacagga
    32821 gatgggctga tgaataataa tgaaaaaatt cttcatgtac ttgatagctt tgaaataatt
    32881 caagaagaac taaaaaaata tcgggatgtt ttggaacaaa gatatgattt tgtcaatcag
    32941 caaaagagta atcacatgga tttcattctc aatatgaatg acttgaagaa gaaattagtt
    33001 gaacgaaaag aacaagaaaa gttgatcaaa gcttactttg aactcggtga aaaagaagtt
    33061 aaaaatgcaa tggaactgaa tgaagagcgc agggttttag atcagctgtt agaacaatta
    33121 ttggtgatgt ttcaaaaagg acgaatagat gaggatttaa ttgaagaagg attgagaaaa
    33181 tatccttcaa actcaggtat cggaatagtg ctgaaagcga ttgatgaaga agaaattgaa
    33241 gctttcatac cgccagagga ttttgaatcg gcaatggaat atataaaata ttatagccaa
    33301 gggatcactg cttttagaga gtttgatcct gaagatgtta ttcatgactt gaacaaccta
    33361 aaagaatggt gtgaaagtta tggagtcgat gattccggat tggactactt gatcagtatt
    33421 atggaaatcg aagaagagat gccggataag cctgatccaa cagatatttt agaattgatc
    33481 catgaagcta gaaatccaat tgcctatatt tctagaggat atactgtatt ggaatactat
    33541 aagccttata tatcagcgat gaaccattta agacgtgtat taagggaaaa acgtgaatac
    33601 cgctcagtgt tgaatgcaac gaatagatta gaaaaagctg tttcagagct agatgcttat
    33661 tatcgtgaac attacctgca agcaggtggt atgccaagaa atacgaaagc aaatatcagt
    33721 aggtatataa aaaaagctga gtaggcggag ttagcccata aaatggcaaa aggtaacctg
    33781 aaaggggcat tttgaatgaa aagggatgtt tttgaattgt acgtattaga acgtctagaa
    33841 gacggcatga tggcacaaga gataattgat gaaattgatg atatgatcag cagatttggt
    33901 caattggagt tgaatgatgg aaacaaaaat tcggtagtgc taaaacaatt ttggttgaac
    33961 aatttttcgt ataaggattt tatcaaagaa aaggcaaata cattgtactg actaaattca
    34021 cgaggagcag tgatgagagc ttattgctaa gagcagttag gctctttttt gtttgtgtaa
    34081 aattatttgt tttataatac acatttatac acaaaaaaat attgacagat agtacacaaa
    34141 tgtggtagga tatatttgta agcaagttag attaacaagt taaaggaaga gcagttatga
    34201 aaaaagaaga actaatagat atatttcaaa ttgttgaacg agcaaataat atgggaatta
    34261 tgttttttga tcgtatttct ttaaagatg
//
LOCUS       DACDYJ010000024        33288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDYJ010000024 DACDYJ010000000
VERSION     DACDYJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.024"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            134..409
                     /locus_tag="IWM51_001197"
     CDS             134..409
                     /locus_tag="IWM51_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP9970627.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            415..1182
                     /locus_tag="IWM51_001198"
     CDS             415..1182
                     /locus_tag="IWM51_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP9970628.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1350..3179
                     /gene="typA"
                     /locus_tag="IWM51_001199"
     CDS             1350..3179
                     /gene="typA"
                     /locus_tag="IWM51_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP9970629.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            3440..5212
                     /gene="recQ"
                     /locus_tag="IWM51_001200"
     CDS             3440..5212
                     /gene="recQ"
                     /locus_tag="IWM51_001200"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP9970630.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            5326..5628
                     /locus_tag="IWM51_001201"
     CDS             5326..5628
                     /locus_tag="IWM51_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP9970631.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            5631..6794
                     /locus_tag="IWM51_001202"
     CDS             5631..6794
                     /locus_tag="IWM51_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9970632.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            6820..10248
                     /locus_tag="IWM51_001203"
     CDS             6820..10248
                     /locus_tag="IWM51_001203"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP9970633.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            10269..11393
                     /locus_tag="IWM51_001204"
     CDS             10269..11393
                     /locus_tag="IWM51_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970634.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            11383..11820
                     /locus_tag="IWM51_001205"
     CDS             11383..11820
                     /locus_tag="IWM51_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9970635.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            11804..12142
                     /locus_tag="IWM51_001206"
     CDS             11804..12142
                     /locus_tag="IWM51_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP9970636.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            12261..12821
                     /gene="rsmD"
                     /locus_tag="IWM51_001207"
     CDS             12261..12821
                     /gene="rsmD"
                     /locus_tag="IWM51_001207"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP9970637.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            12818..13309
                     /gene="coaD"
                     /locus_tag="IWM51_001208"
     CDS             12818..13309
                     /gene="coaD"
                     /locus_tag="IWM51_001208"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP9970638.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            13299..14342
                     /locus_tag="IWM51_001209"
     CDS             13299..14342
                     /locus_tag="IWM51_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9970639.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            14501..15229
                     /locus_tag="IWM51_001210"
     CDS             14501..15229
                     /locus_tag="IWM51_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP9970640.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            15345..15590
                     /locus_tag="IWM51_001211"
     CDS             15345..15590
                     /locus_tag="IWM51_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9970641.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            15587..15805
                     /locus_tag="IWM51_001212"
     CDS             15587..15805
                     /locus_tag="IWM51_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP9970642.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            15941..16480
                     /locus_tag="IWM51_001213"
     CDS             15941..16480
                     /locus_tag="IWM51_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP9970643.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            16473..18746
                     /locus_tag="IWM51_001214"
     CDS             16473..18746
                     /locus_tag="IWM51_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP9970644.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            18784..19830
                     /gene="holA"
                     /locus_tag="IWM51_001215"
     CDS             18784..19830
                     /gene="holA"
                     /locus_tag="IWM51_001215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP9970645.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAKQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(19919..20173)
                     /locus_tag="IWM51_001216"
     CDS             complement(19919..20173)
                     /locus_tag="IWM51_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP9970646.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(20573..20842)
                     /locus_tag="IWM51_001217"
     CDS             complement(20573..20842)
                     /locus_tag="IWM51_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970647.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(21312..21767)
                     /locus_tag="IWM51_001218"
     CDS             complement(21312..21767)
                     /locus_tag="IWM51_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP9970648.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(21905..23833)
                     /locus_tag="IWM51_001219"
     CDS             complement(21905..23833)
                     /locus_tag="IWM51_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9970649.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(24030..24863)
                     /locus_tag="IWM51_001220"
     CDS             complement(24030..24863)
                     /locus_tag="IWM51_001220"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP9970650.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(25055..25759)
                     /locus_tag="IWM51_001221"
     CDS             complement(25055..25759)
                     /locus_tag="IWM51_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9970651.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(25782..26408)
                     /locus_tag="IWM51_001222"
     CDS             complement(25782..26408)
                     /locus_tag="IWM51_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9970652.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            26734..29109
                     /locus_tag="IWM51_001223"
     CDS             26734..29109
                     /locus_tag="IWM51_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP9970653.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(29133..29624)
                     /locus_tag="IWM51_001224"
     CDS             complement(29133..29624)
                     /locus_tag="IWM51_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP9970654.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            29811..30731
                     /locus_tag="IWM51_001225"
     CDS             29811..30731
                     /locus_tag="IWM51_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9970655.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            30885..31229
                     /locus_tag="IWM51_001226"
     CDS             30885..31229
                     /locus_tag="IWM51_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP9970656.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(31419..31919)
                     /locus_tag="IWM51_001227"
     CDS             complement(31419..31919)
                     /locus_tag="IWM51_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9970657.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            32447..32530
                     /locus_tag="IWM51_001228"
     tRNA            32447..32530
                     /locus_tag="IWM51_001228"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32481..32483,aa:Leu,seq:cag)
     gene            complement(32854..>33288)
                     /locus_tag="IWM51_001229"
     CDS             complement(32854..>33288)
                     /locus_tag="IWM51_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP9970658.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11011 a         5771 c         6783 g         9723 t
ORIGIN      
        1 aaaaatcagt tgcgttttgt tttttagaat tatcaataat attattatgt aaacttaaaa
       61 atatgacaac ttcttggttt attaccactt cctatgctac actgaagagg aatatgaaat
      121 aggagagttg aagatgcaag aatatcagta tccacttgat ttagattgga caacagacga
      181 aatggtcatt gtaatgaaga tgtgggaaga tctggaacgt gcgaatgaaa aagggataaa
      241 caatgaagct tttttgaaga cttatcagca gtttaaaact gttgtcaaat ccattggtga
      301 ggaaaaacga ttgggacggg aatttgaaaa agcttccggt tattctttat atcgtacagt
      361 gcagcaagca aaaaaaaatc caaacaaaac tttgagaatg aacgggtgag ataaatggaa
      421 cagatgattg atcatatcaa gtcctggctt gaagaagctg gtgagaaaat caaatcttct
      481 caacaaacat taacaatctc gcaaaaatca aatcgaacgg atctagtaac aaatgtcgat
      541 cgaaacatcc agaattttct aatcgaaaaa atccattcct ttgatccaga ggcgaaaata
      601 ttggcagaag aagaagggtg taatcaatta tcggatctcg atggaagagt ctttatcatc
      661 gatcctattg atggcacctt gaattttgtg attgaagggg aaaatttttg tattatgctc
      721 gctgtttatg aatccggcgt tggaaagctt ggtttcattt atgacgtgat gagaaatgaa
      781 cttttttggg gtggaaaagg aataggcgtg tataaaaatg atgagccatt gcctagacca
      841 gcaaataaat ccttatcgga aggcttactt ggtgtgaata gttatctttt cggtcataat
      901 aaatatggaa tccgagaaat tgcggaaagc agcatgggga cacgtatgta cggatgcgct
      961 ggtcttgaac tagtggcaat cttgaaagga aaccacattg cttatatttc aaaccttagc
     1021 ccttgggatt atgcagcggg aaatgtctta ttggaagagt ttggtatggt aacaagcggt
     1081 ctttttggta ctcctttgac atttgaaaaa agggaatact atttagcagg gacttcttgt
     1141 gcatatgcag aaatcgcaga ccttttgaaa aaagaaggct gaaaaaaagg tagaaatcat
     1201 tcacaaaaat gataaaaacg agcaaaagat taattttctc gaaaaaatga aaacatttta
     1261 ctatatttca tgaaaaaagt ctgctataat tagcagtcga gtaaaatttg ccctgtcata
     1321 aagaaccaat aaaaaaggag ttagttacat tgaattatag aaacgatatc cgtaacgtcg
     1381 caattattgc ccacgttgac cacggaaaaa caacattagt tgatgaacta ttaaaacaat
     1441 cagatacatt agacgcacat acgcaattgc aagaacgtgc gatggactca aatgcgctag
     1501 aaaaagaacg tggtatcacg attttagcca aaaacacagc tgtagactat aaaggtattc
     1561 gcgtaaacat catggatact ccaggacacg cggacttcgg tggcgaagta gaacggatca
     1621 tgaaaatggt tgacggcgtt gtgttggtcg tagatgcgta tgaaggaaca atgcctcaaa
     1681 cacgttttgt attgaaaaaa gcactggaac aacatatcac accaatcgtt gtcgtaaaca
     1741 agattgacaa accttctgct cgtcctgagc atgtcgtaga tgaagttctt gaactattca
     1801 tcgaattagg agcagatgat gatcagttag actttcctgt gatttatgca tctgcactaa
     1861 atggtacgtc aagcttgtca gatgatccag cagatcaaga accaaccatg gcacctattt
     1921 ttgatacaat tattgaaaaa atccctgctc ctgtcgataa cagcgacgaa ccattacaat
     1981 tccaagtttc attacttgac tacaacgatt atgtaggtcg tatcggaatc ggacgtgtat
     2041 ttagaggaac aatcaaagta ggagaccaag tagcattaat taaattagac ggaacagtta
     2101 agaaattccg tgtaacgaaa ttatttggtt tctttggact aaaacgtcta gagattcaag
     2161 aagcaaaagc tggagacttg attgctgtct caggtatgga agatatcttt gtcggtgaaa
     2221 cagttactcc tgtggatcat caagatgcgt tacctatcct tcacattgac gaaccaacat
     2281 tgcaaatgac tttcttagtt aataattcac catttgccgg tcgagaaggt aaattcgtaa
     2341 ccgctcgtaa gattgaagaa cgtttgatgg cagaacttca aacagatgta tccttacgag
     2401 ttgaaccaac aaactctcca gatgcatgga cagtttctgg tcgtggagaa ttgcatttgt
     2461 ctatcttgat tgaaaacatg cgccgtgagg gatacgaatt gcaagtatct cgtccagaag
     2521 ttattgaaaa agaaatcgac ggtgtaaaat gcgaaccgtt tgaacgcgtg caaatcgata
     2581 caccagaaga atatatgggt agtgttatcg agtccttgag cttacgtaaa ggtgaaatgc
     2641 aagatatggt ccatacagga aacggacaaa tccgtttgac attcttaaca cctgcccgcg
     2701 gtttgattgg ttattcaact gaattccttt caatgacacg tggttacgga atcatgaacc
     2761 atacttttga ccaatatctg ccaatgcttc ctggtcaaat tggcggacgt caccaagggg
     2821 cgcttgtttc gatcgataca ggaaaagcaa cgacttattc tatcatgagt atcgaagaac
     2881 gcggtacagt cttcgtagaa ccaggtacag aagtatatga aggaatgatt atcggggaaa
     2941 acagccgtga taatgatttg actgtcaaca tcacgaaagc aaaacaaatg acaaacgtac
     3001 ggtctgctac taaagaccaa acatctgtca tcaaaaaacc aaaacaattg acgttggaag
     3061 aatctttgga attcttgaat gatgatgaat attgcgaagt aacaccagaa agcattcgct
     3121 tgagaaaaca aattttagag aaaaatgctc gtgaaaaagc atctaaaaag aaaaaataat
     3181 ctgtaaaagg atgaagagag aaaccagctt gttataaaaa atagctggtt tctttttttg
     3241 tccaactctt ttctgtggag aagtcatctt tgtcaatgga cataaattca gaaaatacgc
     3301 cgaaagacgt ttaaaagact tttaaaaaac ttatttctag agagtttttg tttcataata
     3361 agataatgtt tcacgaaagg agatcaactt gtccttcgaa gaaaatcgaa gtaaaatggc
     3421 aagagaaagg agcgggaaga tgaacgactt attaaaacaa tactttggct atgatgagtt
     3481 tagacctggt caaaaagaaa ttatccagaa agtcattgat caggaaaatg tattaggaat
     3541 catgcctacc ggaagtggga aatcaatctg ttaccagctt ccagctttac tattagatgg
     3601 gcttactgtg gttgtgtccc ctttaatctc tttgatgaag gatcaggtgg acgcggcgaa
     3661 tcaattaggt atacccgcca cttttatcaa tagctcgtta gatggttacg aaacagctag
     3721 acgcttccaa gaaatcgata gacagcagta ccgtttactt tatatcgctc ctgaacgttt
     3781 tatcatgccg gattttatcc aagcaatgaa gcgatggaac gtccgtatga tcgctattga
     3841 cgaggcacat tgtatctctc agtgggggca tgatttccgc ccaagttatt tacagatggc
     3901 gaaccagcta gatcaactgc caaatcgtcc tgtaattgtt gctttgacag ctacagcgac
     3961 tgtgcaagta gctgctgaca taaaaagact tttaaaaatt ccagaaaaca accatattca
     4021 gactggtttt gaacgagaaa atctacgatt tcaagtaatt aaagatcaaa aaaaagaaca
     4081 atatctaatc gaatatttaa agatcaacaa aaatcaatct ggtatcattt atgcagcaac
     4141 gagaaaagaa gtagaccgtc tctatcattt gcttaaaaaa ttcgattttt ccgtgggtag
     4201 atatcatggt ggtctaaatg agaatgagcg aacagaaatg caagaagcct ttttatacga
     4261 tcgcttgcag ctgattgtag cgacaaatgc atttggtatg ggtataaata aaagcaatgt
     4321 ccgttttgtt atccattatc aaattcctgg ctctctagaa gcatactatc aggaagctgg
     4381 tcgggcggga agagatggtc tttccagtga agccatcctg cttttctctc ctcaagatat
     4441 ccaagtccaa aagtttttta tccagcagtc ccaaagagaa gaagggcaaa agcaaaaaga
     4501 atatgaaaaa ttaaaagcga tgacagaata cgtgtatatt gagtcctgtc ttcagcaata
     4561 catcctaaac tattttggcg agacaagcag tccgtgcaac cgttgtggta actgtttaga
     4621 tgacagagaa atagtagaag taacgacaga agcacagatg gttctttctt gcttaaaacg
     4681 tatgggtgag aactatggta aacaaatgct gatgaaagtc ttagcaggat cgaaagaaca
     4741 aaagctgcaa gctttaggtt ttggacattt atctacctat ggactgatga aaaaccaatc
     4801 gcaaaaagaa acgatgcagt taattgaata tttgatatcg aatggctatc ttctgaccat
     4861 caatggtgag tatcctgttt tgaaagtaac agaaagaggg atacaagtat tgaaaggaca
     4921 agaatctgtt tatcgaaagg aacccaagaa agtccagcag ctatcggatg aagaaaccga
     4981 tacactattt gaagtgctgc gggaactgcg aacggatcta gcatcagaag caggcgttcc
     5041 cccgtatgtc gtattttcag attccacatt gaaagaaatg agccgaatac gtccatcaag
     5101 cagattggag atgcttcaga taaaaggagt tggccaaagc aagcttgata aatatggcga
     5161 agccttttta tctcggataa aaaatgcgga ctctaacgtt ctggataagt agaaagaacg
     5221 aatacgtaaa aatgaataaa aacaactggt taaatctgtt ttttttgctc cttatgaaaa
     5281 tcaaatatgt tataattaag gaattagaat gggaggtaag cacagatgga ttctttgtcg
     5341 aaaaaacttg cgatccaagt attaaatgat gaagcagaac ggatcaaacg attaatccga
     5401 aatcaaaaca acagtctctg tatctcacaa tgcaaagcct tcgaagaagt ggtagataca
     5461 cagatgtacg gcttttcccg gcaagtcggt tttgctgtcc gtataggagt aatcggaaag
     5521 aacgagggcc agctgatgtt aagtgagcta gaacgagaat tgaatcagtt atatagtgag
     5581 gtttaccaag aaatttatga taaaaaagaa gttggcaagg aggaatagac ttgcctaaga
     5641 aagtgaaaaa aaggcatcta ttagactata gcatcttgat tccctattta attttatgtg
     5701 tattgggatt gatcatggta tatagttcta cgtcgtatct attattagaa aacggacaaa
     5761 atccgtccgc atcagtcatt aaccagagta ttttttgggt gcttagcttg attgctatcg
     5821 cactacttta taaaatgaaa acagatgttt tgaaaaacca gcgtttgatc atggcagcca
     5881 tcgcagtttt gacgattttg ctgctgattg ttgtgttttt tggtaaggag atcaatgggg
     5941 cgaaaggttg gctgcaaatt gcaggattct cgatccaacc tgccgaatac ctaaaaatca
     6001 tttctatctg gtacctgtct ctgactttat ctaaaagaca aaacagtgta caaaaagatt
     6061 ttcttgggac tgtgaaacgt ccacttgcaa tggttatcgg acttactgca ttagtcgcta
     6121 ttcttcccga ttttggtaat gcggcagtta tctttttgat tattttggtt ctgcttttag
     6181 ccagcggagt caattatgtg tatactttga ttgtaggagt aggaggcttt tgtctaagta
     6241 cttttacgat ctggctgatc aatatcacaa acggaaagat cttacccgga cgtttacagt
     6301 atatctataa tcgatttgct atttaccaaa atcctttctc agacgaatta aataaagggc
     6361 atcaactagt caacggctac tatgccatgt tcaacggagg actatttggc aggggattag
     6421 gtaacagtat ccagaaaaaa ggatttttac aagaagcaca aaccgatttt atttatgcaa
     6481 tcgtagtgga agaactggga gtgattatgg ggattttgat attagcgttg ctattcttta
     6541 tgattgtgcg cattgtcctt gtaggtatcc gttcaaaaga tccgttcaat tcgcttttgt
     6601 gcattgggat cggcgcaatg tttttgatcc aagtatttgt caatcttgga ggaattactg
     6661 gcgttattcc tttgacaggg atcacgttcc catttttaag tcaaggaggc tccagtttgc
     6721 tgatgctttc gatttgtgtc ggctttgtcc tcaacatcag tgcggatgaa aaacgcaaga
     6781 gcttaggttt ataataaaaa aagatgtagg agtggttcga tgaaaaaagt tttagttgct
     6841 aacaggggtg aaatcgctgt acgtgtattt cgtgcttgta cagaattagg aattaaaact
     6901 gttggaatct atgcagaaga agatgaatat tccgtacacc gtttcaaagc ggatgaagca
     6961 tatttagtag gacaaggaaa aaaaccgatt gatgcgtatc tagatattga agggatcatt
     7021 tctatcgcga aagaatgcgg agctgacgct attcatccgg gttacggtct tttgtcggaa
     7081 aacttgaatt ttgcgcaacg gtgcgaagaa gaaggcatca tttttgttgg acccaaactg
     7141 catcatttag atatttttgg agataaaatc aaagcaaaag ctgcagctat cgaagcgggg
     7201 attgcttcga ttccaggcac agatggacca atcgcttcta tagacgatgc cttggagttt
     7261 gcgaaacaat acggttatcc gattatgatc aaggcagctc taggcggcgg cggacgcggc
     7321 atgcgtgtag ctcacgatga aaagagtgca agagagggct acgaacgtgc gaaaagcgaa
     7381 gcaaaagcag cctttggtag tgatgaagtg tatgttgaaa aatacatcgc taatcctaag
     7441 catatcgaag tacagattct cggggatacg catggaaacg tcatccatct ttttgaaaga
     7501 gattgttctg ttcagcgccg tcaccaaaaa gtagtagaag tagcgccttg tgtctctatg
     7561 aatgaacagc aacgtcaaaa aatctgccag gcagctgtcc aattgatgaa atatgtcggg
     7621 tatgtcaacg caggtacagt agaatttctt gttgaaggag atgattttta ctttatcgaa
     7681 gtcaaccctc gcgtccaagt ggaacatacg atcacagaaa tgattacaga tattgatatc
     7741 gtaacaacgc aactgttgat tgctcaaggt ttggatctgc ataaagaaat cggattgcct
     7801 cagcaagaag gaattaaatt aaatggttca gccattcaat gccgaatcac tacagaagac
     7861 ccgttgaata actttttacc agacacaggg aaaatcgata cgtatcgttc acctggtggt
     7921 tttggcgtac gtctggatgt cggaaatgct tacgcgggct atgtagtgac accgtatttt
     7981 gattcattgc tagtaaaagt atgtacgcat ggtgctacct ttgaaacagc cattcagaaa
     8041 atggaacgtt gcttgaaaga atttagaatc cgtggagtga aaacaaacat tccatttatg
     8101 ctcaatgtca tcacacatcc tgaattccaa tcaggaaatg caaaaacaac cttcattgat
     8161 agtacagcaa cattatttga atttcctcga ctacgagacc gcggaaacaa aacgatgaaa
     8221 tatatcggtg aaattactgt gaatggtttt ccaggtatcg aaagtggcga aaaaccattt
     8281 tatgaagaac cacgtatgcc taaagatcta atcacaagaa ctgattacgt aacggctaag
     8341 aatgtattag atgcgaaggg tgccgatgct ttggtcgaat ggatcaaagg acaggaaaat
     8401 ttgttgttga cggatacgac atttagagac gcccatcaaa gtctgctagc aactcgtgtt
     8461 cggacaaagg atttcaagca gattgcacgt ttgactggag aaggtcttcc tgaactcttt
     8521 tcaagtgaga tgtggggggg agcaacattt gatgtggctt accgcttttt gaatgaagat
     8581 ccttggcaac ggttgagaaa aattcgttct ttaatgccta atacgttgct gcaaatgctt
     8641 ttcagaggtt caaatgccgt tgggtattct aattatcctg acaatgtatt ggtggaattt
     8701 gtcaaagaag cagcagcgca aggaatcgat gtatttcgaa tctttgatag tttgaactgg
     8761 actccacaaa tggaaaaaag tatccaagct gtacgggata ctggtaaaat cgcagaggca
     8821 gctatttgct atactggcga tatcaacgat ccaagtcggg caaaatacaa tgttcagtat
     8881 tataaagaca tggcaaagga actagaacaa ctgggtgcgc atatcattgc aatcaaagat
     8941 atggctgggc tgcttaaacc gcaggcagct tatcgtttga ttagtgaatt gaaagaaacg
     9001 acagatctgc caatccatct gcatactcat gataccagcg ggaatggaat catcacttat
     9061 tctgctgctt caaaagcagg agtagacatc gttgacgtgg caatgagtgc aatgagtggg
     9121 aatactagtc agcctagcat gagcagcttg tattatgcac tggtcaatgg tccacggctg
     9181 ccagaaatca cgattgaaaa tgcacagaaa ttaaaccatt attgggaaga tgtgcgcatg
     9241 tattacaaac cgtttgaaaa cggcttgaat gctcctgaaa cagaagtata tatgcatgaa
     9301 atgccaggag gacagtattc gaatctacag caacaggcaa aagctgtcgg attaggtcat
     9361 cgctgggatg aaatcaaaca aatgtatcat actgtcaact tgatgtttgg cgatatcgtt
     9421 aaagtaactc catcatctaa agtagttgga gatatggcac tatttatggt ccaaaatgat
     9481 cttacagaag aagatattta tgaaaaaggc gaaacactaa gtttccctga atctgttgtg
     9541 actttcttcc aaggagaact agggcaaccc gttggcggat ttcctaaaaa attgcaaaag
     9601 attattctta aaggcagacc tgcattgaat gaacgtccag gtcttttggc tgaatcagtc
     9661 gacttcaacg aagtaaaaaa agaattggca gaaaaaattg gctatgaacc aaaacaagac
     9721 gaagtattaa gctatttgat gtatccgcaa gtctttttgg actatcaaaa agcatataat
     9781 caatttggcg atgttacatt actggacaca ccaacatttt tccaaggtat acgtttgggt
     9841 gaaacaatca atgtacaaat cgaacgcgga aaaacgctta tcattcgttt agatgaaatc
     9901 ggagaacccg atatcgaagg gaatcgcgta ctgttcttca atttgaatgg acaaagacgt
     9961 gaaatcacta tcaaagataa ttcgatcatc agtacggtcc aaacccgatt gaaagctgaa
    10021 ccgacaaacc gagaacaaat tggagcaacg atgtctggtt ctgttttgga tgtattagtg
    10081 aaaaaaggcg ataacgttaa aaaaggtgat acgttgatga ttactgaagc gatgaaaatg
    10141 gaaacggcca tcgaagcacg ttttgacgga gaagtcgcac atgtatatgt gtcttcagga
    10201 gacactattt cgtctggtga ccttttgatt gaagtgactg agaaataata agcgaaaggt
    10261 ggggaaacat gaaaagactg ggtttgtttt ggctgattct gctattagtt attgtggcat
    10321 tctacatgga accagtcttg tttccgccaa aacttgaagt gaaggaaaac tctgatcaac
    10381 aaatatcaaa ccaaatgaca acgtggaaat accaggagct taaagcaact ggtttttcta
    10441 cttatatcgg aaaaccgatc gatcagctgg aaaaggattt tggccaagcc tatgatcgat
    10501 taactagcgg atttggtttt gaaacaagat attatgaaaa taaagctagt catctcacat
    10561 ttgaagcgaa catccaagac agcagagtca caactgttaa agtactgcaa acagggcctg
    10621 ctgatatcgc gccgttttat ttagggatga cgatgcaaga tcttacgaaa atcactacta
    10681 tctatactaa tttcactttt gagtaccatg atacagaagt tggaatcgaa ctaatggaag
    10741 aggatatgaa ttatcgtccg ctaattgctt ttgataacga cacttttgct attttatttt
    10801 ttgatcaaac aacaaacggc ctattttcag ttgtgtattt agataaagat agtcttttga
    10861 aactattgcc atatcaagtg tttggtgaag gccttccaca ttatcaagca gataaatcgg
    10921 ctgattggga atcaatcaat cgcgttaaag aacgaaaaag tacgacttta ttaaatatgc
    10981 ttagaagaga agacgaactt cccatataca atcaaacact tgcctttcaa aaccacagtc
    11041 agctgctact acatgatttt ttgacaaggc ctgaggaaat attgtcagag gaacgtatgg
    11101 ctgattggca aaaatcgcta ggcagtgctc aaacgacagt gaaattctca ctgacgaatg
    11161 atgaattgga gaagttaatc agtaaacaaa aattagaaaa tactagtggg ctatttattc
    11221 atccggcgat tgacgctact ttttcgtttt tatattggta cagcgatcct tacaaccatg
    11281 accgtttcat ggacccggga acggattctg taggcattgc tttttctaaa gaaaatatgt
    11341 tagtcttatt gcaagaagaa aacggagaaa gtagtgatag cagtgatcgt taacgaagag
    11401 ctgtttcaac tggaggatca atgcctccaa gtcgctgaaa tgataaaaaa gagtaaaacg
    11461 atggcttctt atcttgaaca taaacagtca atggaagaat ctgcaaaagt ttgccagttg
    11521 aaagaagcgt tcttgtctaa gaaagaagca tttgaaaatg tagcggcata tggtgagtat
    11581 gcacctgatt atcgtgaaaa aaaacgagct gttcgaaaag caaaaagaca gcttgatatg
    11641 gatgagcatg ttgcagcttt ccgatatagt gaaacacagc tgcaaagtct gttggatcaa
    11701 attgtccaag tacttgccga agaagtttct aatgaaatca aagtagatgc aggtagcccg
    11761 ttttttgaaa cgaaaaaaca ctttggatgc ggaggtaatt gtcatgcaat tagacaatag
    11821 tgaatttgaa ataacaaaac gacgcgggct gatcgtatgg gtctacagct taaaacaatt
    11881 aaagaatctg aaaaagttcg gcttgttgca ttatgtctca agaagaatga aatatgtcgt
    11941 gctttactta aatgaagaga cattggaaga aacagaagaa cgaatccgta aattacattt
    12001 tgttcgaaac gtagaacgtt cttaccgacc ggatatcgaa atgaattttg ccgaaaaaat
    12061 aggaaacaaa acagtattaa aagacgaagg tttcgaaatt gaagagctaa atacagaaat
    12121 ccgattagct gaacatcttt aaaaatctct tatgagcagt ttttaaataa aaaataactt
    12181 ggataacgat cgatttttga tcattgtcca agtatttttt tggtatacta gtaccagtac
    12241 catttctaag gagtgagtag atgcggataa tatcaggaaa ttatggcggg cgccgtttaa
    12301 aaagtttagc aggggcgaac accagaccaa ctacagataa agtaaaagaa tccatattca
    12361 atatgattgg accatatttt gatggagaga cagtattgga tctgtttgca ggaagcggtg
    12421 gtttagctat cgaagcagta tctagaggct gcagtcatgc ggtatgcgta gacaaaaatt
    12481 accaagcgct aaaaatcatc aaagaaaaca tcgaaattac gaaagaacca gagaaattta
    12541 ccacaataaa acttgatgcg gacaaagcaa tcgaacggct agcagcggag aaacaacaat
    12601 tcgactactt gtttcttgat ccgccatatg caaaacaaaa aataatcgat caaatccata
    12661 aaatggaaga actgggactt ttttctaaaa ccgcagttat cgtatgcgaa acggataaaa
    12721 cagtcgaatt gccagaaact gtggctggat tccatcaaat aagaaaacag atttatggca
    12781 taacggccgt aactatctat agaaaagagg gaaatcaatg aaacgtgcat tattcccagg
    12841 aagttttgat ccttttacaa aaggtcactt agatacggtg gaacgtgcgg ctaaattatt
    12901 cgatgaggta gtaattggtg tttttatcaa tacttcaaag aaaagtttgt ttcccccaga
    12961 agaaagaatg acattgatca caaaagctgt ttctcatctt cctaatgtaa aagtcatgca
    13021 tcaagaaaat caattgacgg tagaaacagc aaaagagatt ggtgcagatg cgttgatcag
    13081 aggcatacga agtatcaaag actttgaata tgaacgagag attgctcaga tgaatcatca
    13141 tctatatgat gaattggaaa ctgtcttctt attagcaaaa ccagaatata gtcacgtgag
    13201 ttccagtatt ttaaaagaag tactgcactt tggcggagat gtgagcagct acttaccgcc
    13261 agtgatcaac gaagctttag ctggaaagcg tgaatcaaat gaaagctaaa caatggttga
    13321 aaacagtact gattatcata tttggaatgt tgatcattgg atttgtagta gttcccttgc
    13381 cgtattatgt agaatcaccg ggagccacga tcgatctgaa agaactaatt acggtcaatg
    13441 ataaggaaga taaaggttct gggtctttct ctcttacctc tgttggtatc aggcaagcta
    13501 caggtttctt agctttaaaa gctaaaatga ccccttttga agaattgatc agtgaagaag
    13561 aattgactgg tggggcgaca aatgaagaat ataatcaaat gcaacagtat tatatggatt
    13621 cttcccaaaa tgcagcaatc gaacaagcgt taaaacttgc agacaagcct tataaaatgg
    13681 aattcaaagg cgtatatgtc atgagtgtag agaaaacatc gaatttcttt ggcaagcttt
    13741 cagttggaga tacagtaaca aaagtagacg ggaaaagctt tcaaagtaca gaagcattta
    13801 tggattatgt aaaaagccaa aaagttggac aaacaatcga aatcagttat ttgcataatg
    13861 gtgaagaaaa aacagcttct ggtgatctga tcgagttacc aacagataaa aaggcaggaa
    13921 taggtatcac gcttactgac cataccgaga tcgaatcaga tacagatgtt cgtattaatt
    13981 ctggctctat cggtgggcca tcagcgggat tgatgttcac tttagaaatc tatgaacaag
    14041 tcactggtaa aaacttgcga catggtaagc aaattgcagg aactggaacg atcaacagtg
    14101 aaggagaagt tggccgaatc ggaggaatcg ataaaaaagt ggcaagtgcg gataaagctg
    14161 gcgtagagat tttctttgca ccagatgata cgatttcaga cgatgttaag aaagaatatc
    14221 ctgaaataaa aaccaactac gaagaagcaa aagctgccgc taaaaaattg aattcctcaa
    14281 tgaaaatcgt accagtaaaa acagtacaag aagcgcttga ctatctagag aaaatgaaat
    14341 gaaactaaat aattaaagag gggttgtgac acgagttctg tcacaacccc tctttttcgt
    14401 caaaaatctt cacgataaaa ataaaaacaa aagaaaaaat tgacgtaatt agatgttgaa
    14461 gggacgattt tgccagagct cactgtttct tcaattttaa atgaaaggag taatgaatga
    14521 tttttactgg tcattctttg aaaaaagaac aatgatagaa aaaatcagca agaaaatact
    14581 agcttcaccc aaattgatca tttggatgtc tatgatagtt cttatctttt gtatcgcaag
    14641 tggtttttta ttatcaaatt tgctatttta tagagaaaca gaagagactg ctgcaaaaga
    14701 aatactttct tcgtttagtt ccagtggaga agatccatca gctgaaggaa acgatgtaga
    14761 agaggatgct cgtccaaagg tcatgtacac cgatatcaaa ggaagtgtga aggagcctgg
    14821 tatctattcc ttttcttcag aagaacgggt ctatgatgta ctaaagcgtg caggtggatt
    14881 attagaagaa gcggatagcg atcgaatcaa cttttctgcg aagatagaag atcagcaagt
    14941 cctatatatc cctgctgtag gtgaagagct gccagaacat cttgatcagt tggcatcctc
    15001 ggaaggaaaa cagcctacag cagattccga gcctcccaaa ataaatatca atacagccac
    15061 tccatcagaa cttcagcaga ttcctgggat cggatcagtc aaagcgcaag aaatcattcg
    15121 atttagagaa gaaaacggat ccttccagaa agtggaagac ctgcaggaga tttctggtat
    15181 cggtgaaaag acagttgaaa aactaaaaaa cttcgttaca attaaataga atgattcaaa
    15241 atcgactggc acgagcaata aatggacgct aatggatact aagaataatc caattctaag
    15301 agcttggcag gacgaggagg tagtattttt ggataggaaa tagcttgatt gttcgcaaag
    15361 aaacattaaa aaaaccaatg ctgaatgtct atctccaaaa taaaatatct gggattcata
    15421 ttatgaatac ggctgtcagc ggcaataaca gtcaagctct acgagaacgc tttgccaaag
    15481 atgtacttag ctatacagca gataaagtat ttattctcat tgggacgaat gatctagcag
    15541 agcataaaca actttctaaa gaaacgtacc aaaaaatctg cagtggatga tagttcagct
    15601 gaaaaaagac tatagttctg atcatgcttt ctttatcacg ccaccagcag tcgatgagac
    15661 aaaacaaaaa tatcggaata acaagttgat caaagaatat atctctgttc tcacagaagt
    15721 agcaaaacgc catgattgtg aagtatcgat ttttatacgg aatttcttga acaaatggat
    15781 tcgaagaatt tttataatgg gctaatggat gacggacttc atttcggcag tgaagggtag
    15841 gatcttttgt cagacaggat gattcgttat atttaaagaa tattttgttt aagcaatgag
    15901 tactttttaa tcaacttgaa aaaacggagg aatataaaac atgttagaaa aatcttcaaa
    15961 agcatgcgat acgaataaag aaaaagttac tgataagtca cataatgaac gcattccttg
    16021 ggaccagtac tttatggctc aggcagtact gttatcttta cgtagtacgt gtacacgttt
    16081 agaagtagga gcgacactcg ttcgagaaaa aaggatcatt gctggcggct ataatggagc
    16141 agtcagtgga gatgtccact gcatagatga agggtgttac gtcgtggatg gacactgctt
    16201 gcgaacgatc catgcagaga tgaatgcact cctacagtgt gcaaaacttg gtatcccgac
    16261 ggaaggttca gaaatctatg tcacacattt tccatgtttg gcctgtacaa aggctttgtt
    16321 gcaagctggt gtgaaaggga tcaattatct ccatgactat cgaaatgatc cttatgccca
    16381 agcgttgatc gaacaattag gtgtatctgt acaccaagtt gctttagact cacagcattt
    16441 ttcaaaactt caaaatgaat taacaggaaa acatgtctag cgttttagaa aggcatgttc
    16501 gaaatcggtt gatttttccg gcaattacgt cgatgattat ctgtttgttg tattacactg
    16561 acaattgggg ttatcgtttg cttcttgccg gaattttttt atggttttcg ctaaaagaag
    16621 gacgttttat cgcgtacttg tgtattttgt gtagcataac ggtatccatt tcttataggc
    16681 tacaagagta ttcattttta gaggaaaaaa caacatctgc gcaaattact gtatttacag
    16741 atacgattcg tgcaaatggt aattttttga caatggaagg aaggttatca gagaataaac
    16801 aaaagctcca gctttcttat caagtaaaca cagaagcaga attgaaaaca tggcttaact
    16861 tccgaggaaa gggaatagaa ttgactgctg aaggcgagtt catctctcca gcaaaaaagc
    16921 ggaatcttta taattttgac caagaagctt acttcaggaa ccatagaatt tctggacggt
    16981 tccaaattca aaagattgaa cagatcacca tgcaaaatca ctggcgaaac tggcttcaga
    17041 gaaaaagagg atcttttatc tcctttgtcc aatctgaatt tccgcaaaaa acaagtgtat
    17101 atattaattc tttattatgg ggctataaag atgcaggatt cagagaacca gaagaaattt
    17161 ttagggagag tggtttgctg catctgttca gtctatcggg tctacacatc caattttatt
    17221 tagggtggct gtattattta tttcgacgta tcggatggcc actacacgtc agtattattc
    17281 cgttgagtct actaactatc tgttatgttt ctattgcagg aagttctatt tctgttttgc
    17341 gtgctagttt gctgttttta ttgcgattgc tagtcaaatt gctgaataag cattattcca
    17401 ccatggactt atttgcagtt gtcttttggc ttcttttact atttgatcct cgatttcttt
    17461 ttcatgcggg tggacagctt agttttttca tgagtttttt gtttatcctg atttcttttg
    17521 atggagattg gttgaaaaaa acggcctctc acgtcctgtt tactatgatc acgatgccgt
    17581 tgattgtatg gcatttttat gagtggccta ttttaggtag tctttttaca ttactgattg
    17641 caccggtatt caagtttctt tttcttcctg gtgtgttatt tctcacactc ttcaatcgct
    17701 ggctgccgga aactttactt ttgctgatag aagagggatt aatcttattt gagcgaataa
    17761 tcgatttttc ttcagggtcg tctctcatta taggacgttt gccccttgtg ctatctggga
    17821 tgtatatttt cggattactg atttgtatgg ataggttgaa agaacaacct gtgaaacact
    17881 tgttttatgt tggtcttttt tcttttattt ttttgacttt gccattttta cgcttttctt
    17941 caacgatcgc ttttatcgat gtcggtcaag gagacagtat ctttttgcaa agtccttttc
    18001 gaaaagaaac gattttgatc gatactggag gtcagttaca ctttgaaaga gaaaagtggg
    18061 cgcaaggtat gatacgtcct cctgctgaaa gaaatatcgt tccatatcta aaaggtcagg
    18121 ggataagtgt aatcgataaa ctcatcttga cacacgacga tacggatcat gtgggagaat
    18181 tacccacgtt atctcagcat tttactatca aaaaaattta tatcggttgg ggcgctggac
    18241 gaaatgagcc attacaaagg catttatctg aagctcaaaa aaatggtact gagatcattg
    18301 aaatcaaaca tggggacagg atcagcggtt attatgatct ttatgtattg actccttttg
    18361 aaaagggaga gggaagaaat gaagattcga ttggtctatg gatggaatat aacaataggc
    18421 gctttttatt tttaggagat ttgaatcaag caatggaaaa acaattgctt ttgatttatc
    18481 ctaatctgaa agccgatgta gtcaaattag gtcatcacgg tagccgtacg tcaagtaaca
    18541 cagattttct ttcccatatt gaagcaaaac atgggattat atcctgcggc gtaaataatc
    18601 gttatggtca cccgcacagc gaagtattgg agacactaga agaaaatcag ataaaaacat
    18661 ggcgaacgga tcaacaagga atgatcacct atcgctggca tccagttttt catcctcatg
    18721 ggctagtgga aacaatgata gactagtaat tctcatctgc tcatggtagg ataatttttg
    18781 aaagtggtga gtaatttgag tatacaagca gagttacaaa aaatacgtaa agaaaaactt
    18841 gcatcttgtt acttgatttt aggaacagaa aagtttctcc aagatcaagt tcggacagaa
    18901 attgtcaaaa aaatccaatt agagggcgaa gatgacttaa attttttaag ttttgacatg
    18961 gaaaatgcaa gtttagatga ggtagtagct gaagcagaga cgttgccgtt tttcggagat
    19021 caacggttag tattcgtaga gaatccttat tttttaacag gtgaaaaagt aaacaatgga
    19081 atcgaacaaa atactgattt attgacggct tatttgaaag aaccattaga atcgactgta
    19141 ttagtattct ttgcgccata tgaaaagttg gatgaacgga aaaaagtaac gaaacaattg
    19201 aaaaaaacag caattatcat caatgtccaa cagttgaatg aaaaagaagt acgtcaatat
    19261 ctgatgaata ccttagagaa ttcctcaatc aaaatggaac gaagtgcaat tgatttattc
    19321 ttgaggttga ccgacttaga tttatccaaa atgatgagag agttacagaa attggtcctt
    19381 tatggacaaa atcaacagca aatcacggta agagaagtag aagaattagt tcctaaaacg
    19441 ttagaacata atatttttga catgacccaa tatattttaa aagggaagac ggaacaagct
    19501 ttgcgtttat acgaagattt agttatgcaa ggggaagaaa caatcaaaat taatgcgatt
    19561 ttgctttctc aattacgttt atttttacag acaaaatttc ttgtaaagat tggttatcag
    19621 caggcaaata ttgctgaaac attgaaaatc catccatatc gtgtaaaact tgccaagcag
    19681 gaagtaaaaa aattcgatga acatttgctg gtacagttgt ttgatcaatt agttgagatg
    19741 gattatcaaa tcaaaacggg acaaatcgaa aaagaattaa gttttcaact attcgttttg
    19801 cgcacagggc agttgttaag aaatcgataa gaagaaagac gagggcaaag catgcgggaa
    19861 ggtcgtttgt ttttgtcctt tttttgacaa ataaaaaaat cggccgaagc cgattctttt
    19921 atttagcgat ttttttgctt aaacgagatt tgtcgcggtt agctttgttt ttgtggatta
    19981 gaccttttga ttcagccata tcaatagctt taacagcttc tttatataac gcatctacgt
    20041 tgtcagcacc tgaagctaca gcatcttcaa attttttgat tgcagtacgc atagcgcttg
    20101 tttgagatga atttttcaca ttagcgtttt cgcttgtacg aacacgtttg attgcagatt
    20161 caatgtttgg cattagtttc acctccgata acttaagttc ttacgcgtgt taccgcataa
    20221 ttcaacattc attattatac ctaaaggcag cgtgctttgc aatatgaaca tgacaagttt
    20281 ttttccttaa cagtctttta ttaggatttt ttttcagtaa aaacggatag tacaagctga
    20341 aaaaaatcaa aaagacgcta ttgtgggagg caatagcgtc aaaccaaact agatttttat
    20401 ttatctattt aaacaaagtt tgaatgggaa tgtttggtac taagttatta tataacaaag
    20461 agaaccgttt gcaaagtttt ttttgaaaag ggatacgatt ccctgttgtt ctttgttttt
    20521 ctattaagaa actgcagtta ttttataacc agtatccttt gatcagtaaa ggctattctg
    20581 gtgtaccgat tattttaatg tattcatcga tattttttat caacattttt tttatgacca
    20641 actggttccg acggttccat ttgccactta cttccagcac atatttattt aggggcaaac
    20701 gtaagatata ttttgccaaa tagcgatctt taagcaggcc attgatcaca cggtcctcaa
    20761 gctgcaagtt gatcccagtc agcatcattg gctgttcttg taaaacgtac gtatcaatta
    20821 aaagaccttc aaaagtttcc acaagcgtct tcctttcttt tcaaagtaac ctgaaaacag
    20881 gcactaattc ttccattttg atattcgttg aagtggatgc gatcattctt tcttttgtcc
    20941 actgtaatct agtataacaa aaaagagcag aagagccaaa aagaaaaaag agcatcgaat
    21001 tctataggaa gtctcattgg cacggcctaa attattacaa aacattgatt aaaaaaagcc
    21061 cctgcatcgc aggagcatcc cctatatcgt tttaataggt caatcaaaaa gcagtatcaa
    21121 acacattttg tatcaaatca ggaaataata gataagctaa gcccagaatc aaaagtaccg
    21181 ctagaagaaa ccagccgatt gttcctatga aatactttaa tttttcgaac attgtatcac
    21241 ctcgttgtca gtatatcacg gatcagtaaa ttgggggtgt taagaatctc ctaaaataga
    21301 caaaatttgt tctattttcc taaagcaaat acttgtaaga tacagtaagt gataccagaa
    21361 gcaatgaccg gtccggcggc gattcctctt aagaagacaa cacctaaaat cgtgccgaaa
    21421 acaagtgcaa cagtgatttg tggatcagaa gcaatcaatc ctacaccctt ggaagataag
    21481 acagccacta gcccaccgca aaaaacagct acccagccaa taggggattt aaaggcttct
    21541 aatagatcac gtagaccgat ttctcctgtt gcgataggga ttagaatagc cgctgtgatg
    21601 atcgtcaccc caatattgat tcctttgcct tgaagccaag ggaaaaagga acgggaggct
    21661 ggtatgagct tcataatcaa aacaaaagct gccgctatca tcagactttg atttttagct
    21721 aaaattgcta caagtaaaat aattcctaaa aaaagccaac tttccatgaa atatattcct
    21781 cactttcttc gaaacaatca taacatgttt ttagaaagtt acaaaagtag gaattcatcc
    21841 ttcaagacct attcataagc tgtttttttc ataaaataaa caaaatcata aaaaaacact
    21901 ttgcttattt gacagaagac agccgcgctt tgtatagtgg acaagactta catttttttg
    21961 aagaatcaga aagatcaggt aaaggtctta gtaaacgcaa gccattcaga ataaccaata
    22021 ttgtgcttcc ttcgtgtcct acaactccaa gaggtagatt taaaaattgc aggaagttag
    22081 aagcaatcaa caagagaata acaagaatcg aaaaaataat attttgtttg atgatccgcc
    22141 gcatttttaa agatagccga tgactgtaga caaatttatt taagtcgctt ttcattaaga
    22201 cgatgtctgc gatatccata gcggcatctg tcccttgccc catagcgaca cctaaagaag
    22261 cggctgctaa agcaggcgca tcattgactc catcaccaac catcgcattt acttggtaaa
    22321 gttcattctc ttttttgact agttgtgttt tttcttccgg agtacagctt gcatagtaat
    22381 gttcaatccc tacttgttca gcaatggcct ttgctgtgcc agaatggtcg ccggttatca
    22441 tacttgtatg gatgttttgg gatttaaagt aggaaattac ttgttgggta tttgcttttg
    22501 gaatatctag cagacctaaa acagcaatta actgctgatc tttagaaaga taaatcacgg
    22561 ttttgccttg attttccaaa cgttcgatca tttccgcaat ctctttgctg attatcatcg
    22621 tctcaggatc atatgcgtgc ttgccaactt tccattgcgc acctaaatag gtagtttgta
    22681 atccaaaacc agtaatttct tctattggta agtattggat ttcttcaggt attgtgcaat
    22741 cgaaatattg caaaatcgca tgagcaagtg gatgcgtcgt tttttgttcc atagcaacaa
    22801 gaagttgttg tgccaaatcc ttttcatcaa agaaaaagct gtcagttaca actggttttc
    22861 ctttagtgag cgttcctgtt ttatcaaagg caatcgcttt taattcagaa agcttttcta
    22921 aatggatacc gcctttgatt aaaatcccat tttttgcaga atttgaaaga gcagctagag
    22981 aagctggtgt agcagatgcg acaagcgcac aaggagaagc cacaactaat aataccattc
    23041 ctcgataaaa gctttcatcc caactccaac ctaggaaaaa gtgggggaga aggatcatta
    23101 gaggaacagc aagcaaaatc aattttacat aattattttc gattcgttcg ataaatgaag
    23161 ctgtttgagt tggcgtattc tgcgcttctt ctactaattg gataatctta gcaaagacag
    23221 tttcggaact cgtttttgtt actcgaatcg tgatgggatt tccttgattc agcgttcctg
    23281 catacatctc atcgcctaaa tatttttcta caggcacaga ttcaccgctt aatgcagact
    23341 catcgatcgt tgcctgtgtg ctttctaaaa agccatctat cggaacagct gctcctttag
    23401 cgacaaatac caagttatcc acctttagtt gagaaacatc tacttcttgc atttgtccat
    23461 tttcttttaa taattgggct ttttgtggtt gaagattcat caaagcagta atttcttttt
    23521 gacttttatt agttgtgtat tcctctaatg caccgcttaa acaaaagatg aatgtcaaca
    23581 ttgctccttc aaagtaatta ccgatcaaac aggcaccaat agcagctaaa gccatcaata
    23641 aatccacatt cagtgtccga tcattccaca attctttcaa gccttccgat gtttgtttat
    23701 atccaccgat gataatggca ctgatataaa aaagtggggt gatggtaaga gagaatcggt
    23761 cgaagataaa accgaagatc ataaataaaa ggcagccgat tgttgctgca aaagcttttt
    23821 tctgattagt catcgttatt agacctcctt gtcaaaacaa ggatagcata ataaaaatgt
    23881 aattgataat aataacagct aaatgagaac gataatggtt atcatttggt gattaagctt
    23941 gatgttgttt ttattattta taatgattat tattaaagaa aataaaaaac cagaacattt
    24001 ctcaaataag agaagtgttc tggttttttc tacattgcaa taaaccaata accaatcaaa
    24061 atgataccaa ggataatacg ataccagcca aaagcggtaa agtcgttttt cttcaaatag
    24121 cttagtaaga atttgatagc taagacggaa accacaaaag atacgaatgt tcctacaaga
    24181 agaacaacag tttcttccat tttgaatgaa ttcccttcag ctaaatattt gacgatcttc
    24241 aagccgcttg cgccaaacat gacaggaatc cctaagaaaa atgaaaactc tgtagcgaca
    24301 aaacgcgaag caccaatcaa gatagatcct aggatcgttg cgccagaacg ggatgttccg
    24361 ggaacaagtg ccaagacttg gaataaccca acaagaagag ctgcttggta cgtaaagtca
    24421 ttcagagaag tccaacgagg tgttttgttt ttgttgcgtt tttcgacaat aataaaggca
    24481 ataccgtaaa cgatcaaaac aatggcaact ggtaagaact tgtggaaatt tgcttctaac
    24541 caatcatcca aaggcaaacc aatgatgatg gctggtatgc aagcaacgat cactttaaac
    24601 caaagtgtcc acgtatcttt tttttctacc gttgatttac ttggagaaaa tgggtttaac
    24661 ttgtgaaaat aaagaacgac gacagccaag atcgccccta attggataac gacattgaac
    24721 atcgacataa attgtgaact ggcatccagt ttgatgaatt catctgctag gatcaaatga
    24781 ccagtactac tgatgggcag ccattccgtt attccttcaa taataccgag aatcactgct
    24841 ttgataatat ttgcaaaaaa cataaattcc ttccttccca attaagatac atcttatagt
    24901 ataccgttat tttttgagaa acccaatctg ttttttaata ttaagaaaaa cttgagaaat
    24961 aaagcagata agtacacgaa gagaaaaaag aagacaaagc cctcgttcag actttgcctt
    25021 ttatgattct ctggtaaaaa ttaaaatatc cccattatag aaacgagtcg cgactatgtg
    25081 ttttcccgtt atcgatataa gacgtatcat tgatgccgtg gatagaaaat cgaccgtttg
    25141 cataagtgac ctttgttacg ctgccattta aaagcccagc acgaacaggc tgtttaggat
    25201 cgatcagatt aagcaaaaag gcgatcgtca aaccatgaga tacgactaaa acatttccac
    25261 caccattttt ttcgcagtga tgggcgatat cttcaaatcc tgtccataca cggtctcgca
    25321 atgtttcata agtttccgcc cacccagcgg tatctaagtt gtatacagca ttagcaattt
    25381 gttcaaaaga aacttctgtg gtaaacattt catcatatgt tttaaaattc aatacacgag
    25441 ggatcacacc ccacatttcc atgtcatatc caccttcaag agacccgaaa caccattctc
    25501 taatcctttt ttcaataaaa taaggaattt cttttccctt agaatgttct cctagaataa
    25561 tacgggcagt ttcgattgca cgaccactgt cactagaata agcaaatgaa aagtcgacat
    25621 tttttaaccc gcgacctaaa taatgaatgc cttcttgtcc ttgctttgtg aggggtgtat
    25681 cacaccagcc ttgtactcgt tcaattgtat tgaacatcgt ttttccgtgt cgtacgatat
    25741 aaagtgtagt ttctgtcatt ttattctcct cctaagtcgt tttaacgatt gaaaaaagga
    25801 tttgtccttt tttcctgttc aatcgtcgtt gcatcgccat gtcctggata gactgcaaat
    25861 tcatcaggta atacaaacag ctgggtccga atactatgaa gtaattgttc taaattccct
    25921 gtatacagat cggttctacc gatacttcct ctaaacaggg cgtcgccaga aacgacaaaa
    25981 tcatcaaaaa taaagctgac gcttcctata gaatgccctg gggtagggac gacgcgaaaa
    26041 gacatgtttc ctaaagtata agttttcatt tcaaattcat attctgcagg cgagacaata
    26101 acatcagcaa tatcatcatg tctaccgagt ccagaaagat tcaaaatcgg atcacctagc
    26161 catttttgct ctagtgggct gacataaact gggatttgat aatacgcgcg taaaggttcc
    26221 actgcaccga tatgatcgta gtgagtatgg gtaagcaaaa tcgctaccgg ctgttggttt
    26281 gtttttttga tttcttgttt gattcgttcc gcatcttctc cagggtcaat gatcaaaagt
    26341 gcttcttcgt tgtagaccaa ataacagttt tcttcaatca ttcctgtttt taatctttcg
    26401 atatgcatgt gcattcctcc ttgatagcca aaacttcttt catccattag tataacgcat
    26461 tcattgctag gaaacaaaaa caggtactta tttctcttta cttatctgaa ttatcagact
    26521 atatgaaaag tatttggata ttctaaagag aaggaaagta agttttcata gcattttaat
    26581 atgtccttgt tacattagat tcatccaaaa atccaaattc tatatcctat atccctgcct
    26641 aatcagcagg gtgttttttt atgtttggaa agagagctct ttttgctaaa ctaggttttt
    26701 tcaggtaatc ttagagagaa agaggtggtg gaaatgatca aacagtcaaa aatatctgtg
    26761 cggcacttag tagaatttat tttgcgacgt ggaagtatcg acaatcgtaa aaaaagtaac
    26821 catactgcat tagaaggagc gagaatccac cgcaagctcc aaaaagatgc gggagaaaac
    26881 tatgaaaaag aagtcttttt aaaaacaaca gttcaattag atgaatatta cctgatcgtt
    26941 gaaggccgtg cagacggtat tttcaaaaga gacggtattt attatataga tgaaatcaaa
    27001 acctccgaac cacgatttga agatttagaa ccagaacaag tggaactttt ctttcatcaa
    27061 gcaagggtgt atgcctatat ctatagtcaa gaacatgatt tacaagaaat aaatctgcaa
    27121 ttgacttact tccagacggc agaagaagtg atcacgagaa aggttgaaca tcagacaaga
    27181 gagcagttag aaactttttt taaaaagctg actgaagatt ataaagaatg gcttgttttt
    27241 caagaaaact ggcgcactgt acgcaatgcc tctttgatgg ctttgaaatt cccccatgat
    27301 gagtatagga aaggtcagcg tgaactagcg gtagctgctt ataaaacgat ccgtacgaaa
    27361 cagaaattgt ttgtagaagc acctaccgga acaggaaaaa caatctctac gttattcccc
    27421 gctttgaaag ctgtagggga agaagaggga gaaaagatct tttatctaac ggctaaaaca
    27481 ataactagac aagtagctga agatgctatg acagcattaa aagatacagg agctgaagta
    27541 aaaagtgtca ctttgacggc gaaagacaag atctgctttt tagatgagac gacctgtaat
    27601 ccagatcagt gcccatatgc caatggttat tacaatcgga ttaatgaagg tttatgggat
    27661 cttttgaatc atgagaatca gatcactcgt gaagtcatcg aaacttatgc aagaaagcat
    27721 acactttgtc ctttcgaact atcattagat gtcagtatct ggtgtgacgt aatcattggg
    27781 gattacaatt atttatttga tccaactgtt tatttaagac gtttttttga agatgaaaaa
    27841 aacgaggact atctgttttt gattgacgaa gcacataatt tggtgaatcg ttcccgagag
    27901 atgtattcgg cagaactatc ttatgaaaaa acgaaacgaa gtaaggaagc tataccaaag
    27961 gaattcaaaa aacttcatcg ccgtttcaac aaactattaa aagaatttga ttcgatccga
    28021 gaaatagcaa aagaagatca ttgggattat catcatcaaa aagcaccggc tgaatcttta
    28081 gtaaaggcag gttatcaatt aagcgaaaaa attaaagaat ggctggcaga atttcctgag
    28141 cacccatcac aagaacaatt gcttccttat tattttgatt tgcttcattt tttgaaggtc
    28201 agtgaatttt atgatgatca ctacgaaaca acagtcgaaa aaacgtatcg cgatctgata
    28261 gtaaaagagt tttgtattga tccaagcttg tttcttgagc agtcgttgga taaaggaaaa
    28321 agcagtattc ttttttctgc tagtttttca ccactctctt attatcagga aacactgggg
    28381 ggacaaaaaa gtctggctta caaactgccg agtcctttcc cagaaggaaa ccagcaagtt
    28441 ttcatcgcaa attacctaga gacgacttat cgaaaaaggg aacacagtct ggcaaaatta
    28501 gtagagacga ttcatgcttt tgctgatgga aaggtcggga attatttcgt tttctttcct
    28561 tcttatcaat atttagatct tgcggtggaa gcattcagac agcaatatcc cgacagcaat
    28621 gtgctaatcc aagaaacgga catgaatgag gaagaacgcg aacgattttt aacaaaattt
    28681 caaacaaatc ccgctgaaac attgattgga ttttgcgtac ttggcgggat tttttcagaa
    28741 ggaatcgatt tgagagggga ccgattgatc ggcagtatga tcgtaggtgt aggtttgccg
    28801 cagatgaatc atgagcagga actgatcaaa tcatattatg atgagaagga acaccttgga
    28861 tttgcttacg cttatcagct tcctggaatg aacaaagtct tgcaagctgg tggtcgagtg
    28921 attcgtggca tggcggatca aggaatcgtt gtattggcag atcaacgatt ttctagtttt
    28981 tcttatcgac gtttattccc acaacattgg caaacagcaa gagaagtacg aagtgttggg
    29041 gaattggaag aaggaataca gcgtttttgg ttatcaaaag aagcagtaaa tcaaaaaaac
    29101 gaggaatgac agacgtcagc ctcgtttttt gattaacgat agttagtgaa ttgcaactcg
    29161 attggtaagt caagtttacg taataaagca atcacagctt gtagatcatc acggttctta
    29221 cctgtcactc taatctgatc ttcttggatt tgtgctttta ctttcagttt ttcatttttg
    29281 atagctgtag tgattttctt cgcgttttca cgatcgattc cgttgaccaa atcggcagtt
    29341 tgtcgagctt tgccacctag agcgtgttct gattcggaaa aatggatatt tttgattggc
    29401 acatttcttt tgatcagctt accaaataat acatctttga tctgttcgat tttgaaatca
    29461 tcatcgctca atacaactag attgctgttc tctaatttga tttctactgt agaatccttg
    29521 aagtcaaagc gattggacaa ttcttttgtt gtttgctgaa tcgcattttt tacttcttct
    29581 gtattgactt ctgatacaat gtcgaagcta gcatcttttg ccataggaac cctcctgata
    29641 tacatataaa tatttaccat acatctatct tatcatagga ttagggcaga ttcttacctg
    29701 tttgctaaaa ttcttctcat ttgagttcga agtattttat tttaatagat aaactgttat
    29761 tcttacattt ggaataccaa agtaggggag aggtgcttgt gagtaaaatt atgactgcgt
    29821 atcataaaaa tgaaagatta aagaaaatag tggttatttt gataaccgga ttgaccgctg
    29881 cggtcgcttt aaattatttt ttgattccag ccaatgtctt ttctgcaggg atgaatggga
    29941 tcgctcaaat catttcctcg cttttatatg aatggtttca tatcaagata gacacaggaa
    30001 catttatttt tcttttgaat atccctgtgt ttgtattagg gtttctcaaa gtaggaaaac
    30061 gagcaacgat ccttagtttt gtgaatgttg tatgtgtatc ggtgatgacg atgctgcttc
    30121 cagtcggaca gatcactgat aatattttga tgaatgccct agtaggcggt gttttattag
    30181 gtcttggcgt aggattatct ttaaaaatgg gctttacaac tggtgggatg gatatcatct
    30241 ctcttgtact ttcacaaaca actggaaaaa cagtcggaaa ctacatgctg attctaaatg
    30301 gtatcattgt agtcatcgca ggcttcttct ttaactggga aagtgccttg tatacgatta
    30361 tttccattta tgcaatgact caagtaattg atgccatcca cacgagtcat caaaaagtaa
    30421 cagcaatgat cgttaccgtc aagccagaag aggtaacaga agaactagca aaacggatgg
    30481 tacgtggaat gacattactg ccgtctgtcg gtggctattc aaaaaaacaa gggaacatga
    30541 tcatgatggt catcacccgc tacgaaatgt atgatcttga tcagatcgtt catgaaatcg
    30601 acgacgatgc atttatcaat attttaccaa ctcattcagt atttggccgg tttgcaaatg
    30661 aaaacgaaca aaaaatgtat cgaacaactg gcgtattgcc agaaataaaa aatcgttcac
    30721 aaaaaaaatg aggaatcagg tttattttag aataaattta atctattaca tctaataaga
    30781 acatttttat aaatttaaac gagaatctta cttctaaatt ctcatttaca agcagctaaa
    30841 aaaagagtat gataaataaa aatcgtgtta aggagtgtcg tacgatgagc gaagcaaacc
    30901 aagaacggtc tggacaagaa atagaactaa tcaaagaacg tattcttgct gccttggaaa
    30961 tggtgattga tcctgaatta ggaatcgaca tcgtcaattt aggcttgatt tatgatatag
    31021 aattcaatcc tgaaaatgga gaaacagtca ttaagatgac tctaacaact atgggctgtc
    31081 cattagcaga tattttaaca gaatctatcc atgacgcatt aaaagaagtg ccagaagtaa
    31141 cgaaagcaga agtcaaacta gtctggtatc cagcatggac gacagataag atgagccgtt
    31201 atgcacggat tgcgttgggg atacgataga aatcttggct gactagctat gagaagcaaa
    31261 taggaaaaac aaaaaagcct tttatccttt ctagaagaaa agagataaaa ggtttctttg
    31321 tttataaaat tttgtctgct atattataag ttaattaaat aaatgtgttc tattgataaa
    31381 ggacattcaa atccctttcc ttttggaatc cttctttttt acatcagttt gattttcgta
    31441 tagtattctt tctcatcttc acgataaatc gcagccccat ttttttgttg tgctttaaga
    31501 ctgccaagat tttttttatt tacagacaaa agtgcctcat caatgccgat tttttttgcc
    31561 tcttgtagta ctaatgctaa tccttgtgtg gcatatcctt ttttacgata attaggggag
    31621 atgccgtaac caatatggcc agcgccgttt cttaatgttt cgttcagttt atggcgcaaa
    31681 ttaaaaattc ctacatactc atgttcatca ctttctaaaa taaagacggt tgcgggaacg
    31741 taactctcag gaagatcgat tcctaaagac gttttttcgc atttttttac ataatctaaa
    31801 aattcagcaa atgtatagcc ataagcattg ttgataaatc cactctcatt tggtccaaag
    31861 ctagtttgta gctgataagc tttctttaaa tctgcttccc atagtttgat cagtttcatc
    31921 gtattcacct ctttaagtaa ccttattcat ttgttttcta ctatagcact attgttgtaa
    31981 atagccatgc ctatttttat ttattgtgtc atatcgtatt aagaatcatc gaacaataca
    32041 aatatgaatt acaataaaaa aattatttga agttcgttca ttcgttactt atgatctcta
    32101 gaagaaagaa aacgtgaatt acttcagcgt ctagaattcc agaaggatag taatcaacag
    32161 aagaaataat caatatccaa atgaaagaag atagctgaat cgagaaaaag aggaaaaaag
    32221 aaatgaagaa aaaaataaaa tagttactaa tcccagagtc gttcgtttaa cattgaaatc
    32281 gatcatatgg ataaaacaga ttttttcacc aaccctcaat agatgatgga gttgctcgga
    32341 tgaattattt gtgtagatgg cataagggag ggcgtacaga aatgaaaaag aacaaaaata
    32401 tgttcttgac aaaaaacaca tgtaatccta aactaaaaga tgtgatgcgg ctatggcgga
    32461 attggcagac gcgctagctt caggcgctag tgagggtgac ctcgtggaag ttcgagtctt
    32521 cttagccgca taatttgttt ttttataaag agaaggtaca cctggagcga tgagaaagaa
    32581 aataacatat tatctaaaag aatataggaa aaattttaaa caacggaacc agattcaaat
    32641 aaatcaaaaa aaatttgtgt gaaggtggga cagtttaaaa cctaatagat tgattcaaat
    32701 agtagattta tagattctga tacaagaaat actggcaaac gatagttaag gaataaagac
    32761 taaacttaaa ctcaaaaaga ggtcatgcga aagaataaat cgcatgacct cttactatat
    32821 tttgaatgtg tttaaacgtt caaataatga atcttagtac caaccgtttg cttcccagaa
    32881 tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg ctactctttc
    32941 ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt aacggccgat
    33001 gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga tccattcttt
    33061 tgcagagctt gtacttgctg gagtagcagc agtttctgtt acagcaggag caggttgtgt
    33121 ttcagcaaca ggcgctgctt gtgtttccgt ttgaaccgtt tcttgtacaa caggttgttc
    33181 agcttgaaca ggttcagctt ggactggtgc ttcttgaacg gctgcttgag gttcagcagc
    33241 tgcttgtgca gagccttctg ttgggattgt taattgttgt cctgagta
//
LOCUS       DACDYJ010000025        32968 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDYJ010000025 DACDYJ010000000
VERSION     DACDYJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.025"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            164..2698
                     /gene="secA"
                     /locus_tag="IWM51_001230"
     CDS             164..2698
                     /gene="secA"
                     /locus_tag="IWM51_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP9970659.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDIQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGDE
                     DAVIQSKMLTKQVEAAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIVDFAGSTLVHEDSISLTDIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKDAN"
     gene            2825..3926
                     /gene="prfB"
                     /locus_tag="IWM51_001231"
     CDS             join(2825..2887,2889..3926)
                     /gene="prfB"
                     /locus_tag="IWM51_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP9970660.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAVMQEEAEARI
                     NALDEKMNTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4031..4717
                     /gene="ftsE"
                     /locus_tag="IWM51_001232"
     CDS             4031..4717
                     /gene="ftsE"
                     /locus_tag="IWM51_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP9970661.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4701..5594
                     /locus_tag="IWM51_001233"
     CDS             4701..5594
                     /locus_tag="IWM51_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9970662.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEKAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFYQLINPSLLRSHYSLIHPENIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5746..7194
                     /gene="cls"
                     /locus_tag="IWM51_001234"
     CDS             5746..7194
                     /gene="cls"
                     /locus_tag="IWM51_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9970663.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDTLGE
                     EVREALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPEFFPTIHSTGDMAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEVYENRGMMIKMKEGLARLISPLL"
     gene            7531..8388
                     /locus_tag="IWM51_001235"
     CDS             7531..8388
                     /locus_tag="IWM51_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP9970664.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQAENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8503..9423
                     /gene="pstC"
                     /locus_tag="IWM51_001236"
     CDS             8503..9423
                     /gene="pstC"
                     /locus_tag="IWM51_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP9970665.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9423..10307
                     /gene="pstA"
                     /locus_tag="IWM51_001237"
     CDS             9423..10307
                     /gene="pstA"
                     /locus_tag="IWM51_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP9970666.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10322..11128
                     /gene="pstB"
                     /locus_tag="IWM51_001238"
     CDS             10322..11128
                     /gene="pstB"
                     /locus_tag="IWM51_001238"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9970667.1"
                     /translation="MKKYNWNETNVITMPKDKEVALYTNDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYKDVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11144..11902
                     /locus_tag="IWM51_001239"
     CDS             11144..11902
                     /locus_tag="IWM51_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9970668.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWEDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11915..12592
                     /gene="phoU"
                     /locus_tag="IWM51_001240"
     CDS             11915..12592
                     /gene="phoU"
                     /locus_tag="IWM51_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP9970669.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12814..13848
                     /locus_tag="IWM51_001241"
     CDS             12814..13848
                     /locus_tag="IWM51_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9970670.1"
                     /translation="MQKKISIIITLFFICILASWFIQRQAALYDVPVIEIERVRIEED
                     QQLITGRLMNTDQKGEVISLTAPYQKNQIETANITTRQIYLVQMHGDEWQLVAKKNDG
                     WLFFFTSVFIGTMLLIGGKQGVFALGSLIINIVLLLLLLLLFTKTEGISLLNIAILFV
                     VIGIIVSILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTR
                     PYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKQQPKISAKQLWLSGRQVGRDVIGS
                     MANILLFVYISGAIPSLLLYLGNQWSFKETVERHLSLELLRVIAGSLGIVLSIPIAVL
                     FFLAVRRLKK"
     gene            13845..14612
                     /locus_tag="IWM51_001242"
     CDS             13845..14612
                     /locus_tag="IWM51_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9970671.1"
                     /translation="MNVLIILVGIFAISVLFVGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILVLSIIGFLLIAVVILFFINGYNLKMRAAFASILIFLFCFLLLIPITDFLAI
                     QGFTSIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     MSFNQLRHASFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDISFQQIINEKSFVL
                     EYTMAILTTLAALLVLPLTCVTTAYLFTKKKEHLKME"
     gene            14712..16280
                     /gene="liaX"
                     /locus_tag="IWM51_001243"
     CDS             14712..16280
                     /gene="liaX"
                     /locus_tag="IWM51_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP9970672.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDELENGEEKISEPVDPKEKERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEATGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSDDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVNQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATPENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDSIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16459..16764
                     /locus_tag="IWM51_001244"
     CDS             16459..16764
                     /locus_tag="IWM51_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP9970673.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF"
     gene            16764..17114
                     /locus_tag="IWM51_001245"
     CDS             16764..17114
                     /locus_tag="IWM51_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP9970674.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
     gene            17280..18218
                     /locus_tag="IWM51_001246"
     CDS             17280..18218
                     /locus_tag="IWM51_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP9970675.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18233..19063
                     /locus_tag="IWM51_001247"
     CDS             18233..19063
                     /locus_tag="IWM51_001247"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP9970676.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGSLPDYDRSLGKNQQII
                     "
     gene            19107..20132
                     /locus_tag="IWM51_001248"
     CDS             19107..20132
                     /locus_tag="IWM51_001248"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9970677.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTNV
                     KAAKYVQELFMNHYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20147..21085
                     /gene="galU"
                     /locus_tag="IWM51_001249"
     CDS             20147..21085
                     /gene="galU"
                     /locus_tag="IWM51_001249"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9970678.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            21210..21659
                     /locus_tag="IWM51_001250"
     CDS             21210..21659
                     /locus_tag="IWM51_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP9970679.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFLKTKNILKRQEENKNG"
     gene            21652..22266
                     /locus_tag="IWM51_001251"
     CDS             21652..22266
                     /locus_tag="IWM51_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9970680.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(22329..23432)
                     /locus_tag="IWM51_001252"
     CDS             complement(22329..23432)
                     /locus_tag="IWM51_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9970681.1"
                     /translation="MNNVKINELRQWMAQHQLELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            23642..24643
                     /gene="ccpA"
                     /locus_tag="IWM51_001253"
     CDS             23642..24643
                     /gene="ccpA"
                     /locus_tag="IWM51_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP9970682.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            24997..25392
                     /locus_tag="IWM51_001254"
     CDS             24997..25392
                     /locus_tag="IWM51_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970683.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREAQKQQDLLAVEKQS
                     VKVLKNTFADIKEVKIEQFEKNDMTGIYEALITMTNTEGKAVYFDYSFTKESNEIDGY
                     GVENEEVQKEGITVDEIIVIYTDGSEEKV"
     gene            25389..26645
                     /locus_tag="IWM51_001255"
     CDS             25389..26645
                     /locus_tag="IWM51_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAP9970684.1"
                     /translation="MTITDKQYKGISNKVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKNSFDGMQAMAVAPIKNGKVDISQVVIAFAGTNLSDWKDLETDARSVMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAYLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSVKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            26657..26980
                     /locus_tag="IWM51_001256"
     CDS             26657..26980
                     /locus_tag="IWM51_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970685.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            26973..27239
                     /locus_tag="IWM51_001257"
     CDS             26973..27239
                     /locus_tag="IWM51_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970686.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQTWAEKRLTDQNHQ
                     MTAEEATQEFSNRHAHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(27402..29747)
                     /locus_tag="IWM51_001258"
     CDS             complement(27402..29747)
                     /locus_tag="IWM51_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9970687.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKDEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            30026..30928
                     /locus_tag="IWM51_001259"
     CDS             30026..30928
                     /locus_tag="IWM51_001259"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP9970688.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            31139..32914
                     /locus_tag="IWM51_001260"
     CDS             31139..32914
                     /locus_tag="IWM51_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP9970689.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPTVEIIIA
                     CLLAIYYRKYNSSKKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFDFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVIGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIAVTSMLVFSKLNLKKALSSK"
BASE COUNT        10815 a         5843 c         7066 g         9244 t
ORIGIN      
        1 ttttttgagt ttcctcctct tttagatgaa atatgaataa attatgaagt tcttacttta
       61 atacgcttaa gatatttcaa ttatctataa ctaatgataa aatgaaaagt aaagtctaca
      121 tagtctggat aatacacaga aggaaaagga gattacacaa cgcatggcca atttgattcg
      181 ttcacttatc gaaaacgata agaaagaact aaaacgttta gatagcattg caaaaaaagt
      241 tgaagcatac gctgaccaaa tggcagcatt aactgatgaa caattacagg caaaaactgc
      301 tgaattcaag ggcagatacc aaaaaggaga aacattagat caattacttc cggaagcttt
      361 tgcagtcgtc cgagaagcag caaaacgtgt attggggtta tatccatacc acgtacaatt
      421 aatgggtggg atcgtgcttc acgatgggaa tatccctgaa atgcgtactg gtgaaggtaa
      481 aaccttgact gcaacgatgc cggtgtatct gaatgcgttg actggcgaag gggtccacgt
      541 agttaccgtc aatgaatatt tagcaacccg tgactcgacg gaaatgggcg agctttataa
      601 tttcttagga ttgacagttg ggttgaatat caactcaaaa tcttcggatg aaaaacgcga
      661 ggcttataat tgcgatatca cttatagcac gaataatgaa ttaggatttg actatttgag
      721 agacaacatg gtcgtttacc gtcatcaaat ggtacaacgt ccgctgaatt atgccattgt
      781 cgatgaagtt gactcgatct tgattgatga agcgagaacg ccgctgatca tttcagggca
      841 agcagaaaaa tcaacagctt tatatacaag agcagacaac ttcgtgaaac gtttgaaaga
      901 agaagaagat tataaaatcg atatacaatc aaaaacaatc agtttaactg aagcagggat
      961 cgaaaaagct gaagaaacat tcggtttgga caacttatat gacattgaaa atactgcatt
     1021 gacccatcac ttggatcaag cattgagagc caactacatc atgcttttag acatcgatta
     1081 tgttgttcaa gatggaaaag tattgattgt tgaccaattt acgggtcgga tcatggatgg
     1141 acgccgttat tcagatggtc ttcatcaagc gatcgaagct aaagaaggcg tagagatcga
     1201 agacgaaacg aaaacgatgg cgacgatcac tttccagaac tatttccgta tgtacaagaa
     1261 acttgcgggg atgactggta cagctaagac agaagaagaa gagttcagag agatttataa
     1321 tattcaagta taccagattc cgacaaaccg tccggtcatc cgtgacgatc gaccagattt
     1381 gctttaccca acattacaaa gcaagttcca tgctgtcgta caagatatca aagatcgcta
     1441 ccgcaaagga cagcctgtct tagtcggaac tgttgcagtt gaaacatctg agttattatc
     1501 agatatgctg aatcaagaac gtattcctca tgaagtactg aatgcgaaaa atcactttaa
     1561 agaagcagaa atcatcatga atgcgggtca aaaaggtgca gtgacgattg ctaccaacat
     1621 ggctggtcgg ggaacagata tcaagctagg gcttggcgta agagaacttg gtggcctagc
     1681 agttatcggg acagaacgac atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc
     1741 aggacgtcaa ggagaccctg gtatgtcaca attctacttg tctttagaag atgatttgat
     1801 gaaacgattc ggttcagaac gaatcaaagc tttcttagat cgaatgaaga tcggtgacga
     1861 agatgctgtg atccaaagca agatgctgac gaaacaagta gaagctgcac aaaaacgcgt
     1921 agaaggaaac aactacgaca cacgtaagaa cgtcttgcaa tacgatgacg tgatgcgtga
     1981 gcaacgtgaa gtaatctata aacagcgtca agaagtgatc atggaagaaa aaagcttatc
     2041 taaaccactg atgaatatgg tcaaacggac aatcagtcgt gtggtagatg cgcatacgca
     2101 attagaaaaa gaaaattgga atttggaaag tatcgtcgat tttgctggca gcacgcttgt
     2161 ccatgaagac agtatttccc ttacagatat cgaaggaaaa acctctcaag aaatcaaaga
     2221 atatctaaac gaacgagcac aagaagtatt caataccaaa gcagctcaat tgaacggacc
     2281 agagcaactg cttgaatttg aaaaagtcgt gatcttacgt gtcgtggact ctaaatggac
     2341 agatcatatt gatgcaatgg accaattacg tcagtcaatc ggcttgcgtg cctatggtca
     2401 aaacaatcca ttagtcgaat accaaacaga aggttataag atgttcgaag atatggtagg
     2461 tgccattgaa tatgaagtca ctcgtatctt catgaaagct gagatccgtc aaaacgtaca
     2521 acgtgagcaa gtttctcaag gaacagcttc tcactcagaa gatggagatg ccaaagaaaa
     2581 cagtaatact agcgcgaaaa aacaaccaat ccatgtgaac aaggttggta gaaatgatcc
     2641 ttgtccttgt ggaagcggga aaaaatataa gaactgtcac ggaaaagatg caaactaatt
     2701 tctgacagca ggattggtga gacaaattct tttttaaaga atttgtctca ccttctttct
     2761 gttatttgac gattctgtta gactagatat aataaaaaaa gtatagtgaa agaaggaaaa
     2821 aaacatggaa atatccgaaa ttcgaaatct cctgacagaa atgcaggaaa aggtcacaag
     2881 cttcaggagg tctctttgac ttagaccagc tggaagaaga cattgcggaa gcagaagcaa
     2941 aaatggctga acctggtttt tgggatgata gcgaagctgc tcaaaaagtg atcagtgaaa
     3001 acaatgcaaa taaagaaact tatgatcaat tccattctct tgctgaagaa ctggaagaaa
     3061 tcgaattgat ggtggagatg ttgcaggaag aacctgatgc agtaatgcaa gaagaagcag
     3121 aagcacgtat caatgcgcta gatgaaaaaa tgaatacgta tgaattatct atgttgttag
     3181 atggtcctta tgatcgaaac aacgccatcg tcgaattgca tccaggggct ggcggaacgg
     3241 agtcccaaga ctggggcagc atgcttttac gcatgtatac acgctgggca gagcagcatg
     3301 ggtatcaagt agagacacta gattatcaag caggcgatga agcaggaatc aagagtgtga
     3361 ccctactgat caaaggacac aacgcatttg gctatctgaa atcagaaaaa ggtgttcatc
     3421 gccttgtacg tatctcgcct tttgactcag caaaacgtcg tcatacttct ttttgttcgg
     3481 tagatgtgat gccagagctt gatgaaacga tcgatataga aatcaatcca gatgatttga
     3541 agatcgacac atatcgggcg agtggtgcag gtggtcagca catcaataag acagaatccg
     3601 ctgtacgaat cactcacttg cctacaggaa cagtagtagc gagtcaggca caacgttcac
     3661 aattgaaaaa ccgggaacaa gcgatgggga tgctgaaagc taagctttac caattagaag
     3721 tggaaaagaa agaacaagaa gcagctgcac tgagaggaga acaaaaagaa atcggctggg
     3781 gatcacagat ccgttcttat gttttccatc catattctat ggtaaaagac catcggacaa
     3841 acgtagagac aggaaatgtc caagctgtta tggacggaga tcttgattta tttattgacg
     3901 gctatttgaa aatgcatctg caataagagc cctttgttaa attgaaataa aattgtaaga
     3961 ttttgcaagc aagttgaaac attgccatgc tataatagct aaagactgaa aaagatatgg
     4021 agagaacgcc atgattgaaa tgaaggatgt aatgaagaag tactcaaacg gtaccactgc
     4081 tatccgcaat ctctcgattg tcatcgaaca aggagagttt gtgtatgttg ttggtccgtc
     4141 tggagctggt aaatctacat tcatcaaatt gatgtatcga gaagaaaaag cttcaaaagg
     4201 aactttgaac gtcgcaggtc atgatctaat agccatcaaa aaccgcgaag ttccttatct
     4261 acgcagagaa atcggcgtgg tgttccaaga ctataaatta ttaccaagaa aaacagtata
     4321 tgaaaatgtt gcgtacgcca tgcaagttat cggcagaaaa cctcgggata tcaaaaaacg
     4381 agtaatggaa gtgctggatc ttgttggttt gaaacacaaa gtccgagtct ttcctagcga
     4441 attatctggt ggggaacaac aacgtgtttc tattgcccga gcaatcgtaa atacacctaa
     4501 agtacttatc gcggatgaac cgacagggaa tctagatccg gaaaactcat gggaaatcat
     4561 gaaactatta gatcggatca atgcacaagg aacaactgtc gtgatggcta cacataacag
     4621 tacgatcgta aatacgattc gacaccgagt aattgctatc gaaaacggcc gcattattcg
     4681 agaccaagcg gaaggggaat atggatacga tgattagaac ctttttccgc catttattag
     4741 aaagtatcaa gagtttgaaa cgtaacggct ggatgacagt agcttcggtc agtgcggtaa
     4801 cgatcacgtt gactttagtt ggtgtgttca tggcggtcat catgaacgca accaaactag
     4861 cacaagatat agaaggaaac gtggatgtat ctgtgttcgt agacattggt acaaaagacg
     4921 aagagatgaa aaaattagaa aaagaattaa atgacttaga tcatgtcaaa aaagtcgaat
     4981 tttcaagcaa agaacaggaa ttgaagaaaa tccaaaacga aatgggcgat tcttggaaat
     5041 tgtttgaagg ggataataac ccgttatatg atgtatacgt agtaagtgct accgatcctt
     5101 cttatacaaa acaagtagca gaaaaagcgg cagatttgaa gaatgtttcc cgttctgatt
     5161 acggtggggc ttcctcagat cggattttcc aaattgctgg tgcagttcga acatggggat
     5221 tagttgcggc ggcattgctg ctgtttgtag caatgtttct catttctaac acgattcgga
     5281 tcacgatttt atctagacag cgtgaaattc aaatcatgcg tttagtaggg gcaaaaaatg
     5341 gatatatccg ctggccattt ttccttgaag gtggttggat cggcttaatc ggtgccattc
     5401 ttccgatact gatcattaca tttggttacg catggtttta ccaattgatc aatccgtcat
     5461 tattacgatc tcattattca ttgatccatc cagaaaatat cgtttggaaa atcaatcttt
     5521 tgatggtcgg tattggtgtg atcattggtt cactcggctc gatcatctct atgagacgtt
     5581 tcttgaaatt ctagtgtcac aagataaata taatcagaaa gttggacgat ttcgttccag
     5641 ctttcttttt ttatcagcta ggtggagtaa atggggcggc ttttttctct ttactttaaa
     5701 aaacaacttg ggtattctta agttggtaga aaaagaggtg aagccgtgaa gattatcgtt
     5761 gacaattttt ttacgatcct tttgattatg aatattttgc tttcctttat tatcgttttt
     5821 cgagaaagaa aggaaactgc gcaaacatgg gcatggctgc tcgttttgat gttcattcca
     5881 gttgtaggat ttattttata tattttttta ggaagaggga tttccaaaga gaaaatcttt
     5941 gatttgaaga tccaagggaa aatcggtaag aatctagaaa tcgaagaaga taaacaagca
     6001 ttgatgcgag ggctttatcc tcatccgcct actggacaag tagatgtgaa acaattgatc
     6061 tatatgctta ctgtatttga aagtacgctt tatacgacta aaaatgaaat cactttattt
     6121 acagatggtc gtgaaaaatt cgatgcgctg atcgaggata tcaaacaggc agaagaccat
     6181 atccatttcc aatattatat ttaccgcagt gatacattag gtgaagaagt tcgagaggca
     6241 ttgacagagg cagcaagacg cggagtaaaa gtcagagtcc ttcttgatgc atggggttcc
     6301 acacaagttt cttctagttt ttttcagaat ttgaaaaaag caggtggaga agtcgcattt
     6361 ttctttcctc tattcgttcc gtatatcaat cctcgaatca attatcgcaa tcatcgtaaa
     6421 atcgtagtga ttgatgggaa aatcggatat acaggtgggt tcaatgttgg aaacgaatat
     6481 ctaggattgg taaagaaatt tggttattgg cgagacaatc atctgcgtat ctatggagaa
     6541 gcggtttaca ccttgcaaaa ccgttttttg atggactgga attctcagca tacaaaagaa
     6601 gtaggatatt caccggaatt tttcccgacc attcattcta caggtgatat ggctgttcaa
     6661 atcgtgacaa gcggaccaga taccgaacat gagcaaatca aaatgacata tttgaaaatg
     6721 atttcgttag ccaaacggga gatattgatc caaacgccgt attacatacc ggatggatcg
     6781 attcatgagg cattgaagtt ggctcttctc tcaggcgtaa aagtccatat ccaaatacct
     6841 aacaaaccag atcatcttct cgtttactgg gcaacgtatt ctttcgctgc agagctgatc
     6901 gagtatgggg caagaatcga aacatatgaa aatggattta tccatgcaaa gacgatgatt
     6961 atcgatggcg gcatcgtttc cgttggttca gctaatatcg acgttcgctc atttagattg
     7021 gattttgaag tcaatacact gatctatgat gaacgaatgg cttctcgcgt aagaaaagca
     7081 ttctttgaag attcaaagat ttcgacgcat ctcacaaaag aagtgtatga aaaccgaggg
     7141 atgatgatca agatgaaaga aggattagca agactgattt cgccgctcct ttaaaataca
     7201 aaaaggacag gagagccatc aatagatgga aatctcgcgt ccttttttct cggggccatt
     7261 tctgagagta cgtcagaaat ggagaaagcg tctcgcatga gcgaaattaa ctttggggta
     7321 tctcttgttt tttggggaaa gcaagaaata agctaatata tgctattaaa agagacacat
     7381 ttcaaagcca gtatagcata gaaactttta atgagatagc tttttttgca attatgagaa
     7441 tttattttgt tatttaatga ttttgattcg ttttcatcaa gtaatgaaaa tgatagaata
     7501 aacatacttt aggattaaag gagatttcta atgaaaaaac taaaattatt cttaccaata
     7561 ataggattag ctattctttt atcaggctgt tcaaagtgga ttgatcgagg agaatccatt
     7621 actgcagttg gctcttctgc cttacagcct ttagtagaaa cggttgctga aacctaccag
     7681 gcagaaaatc ctggaaaatt catcaatgta caagggggcg gaagcggaac tgggttgtct
     7741 caagtacagt caggagcagt cgatatcgga aattctgatt tgttcgcgga agaaaaatca
     7801 gggattgatg cttcggcatt agtcgatcat cgagtagcag ttgttggaat cactcctatt
     7861 gtgaacaagg atattggcgt aaaagatatc agtatggaaa acctgaaaaa attatttacg
     7921 ggcgagatca aaaactggag tgaagtcggt gggaaagatc agccagtagt tatattgaac
     7981 cgtgcatcag gaagcgggac ccgtagtacg tttgaaaaat gggtattgga cggagaaaca
     8041 gctgttcagg cacaagaaca agattcaagc ggaatggtgc gccaaatcgt tgcggacaca
     8101 cccggagcaa tcagctatgt tgctttttct tacgtttccg atgatgtgga tacgttgaac
     8161 gttaacggtg tggaaccaac agatgaaaat gtgacaacga acaaatggac gatttgggca
     8221 tatgagcata tgtacacaag agaccatcca aaaaaactga caaaagaatt tttagactat
     8281 atcttatctg atgaagtaca agataatatt gtcggagagc taggctatat ccctgtttca
     8341 catatgaaag ttgaacgtga ttggcaagga aacattgtta aagagtaatc tttacacaaa
     8401 atttacaaac ttagctaaac ttttacatgt tattaaaacc aaattgatgt tcaatttgta
     8461 cactgagtga gtatgttaaa cctgattaag ggggagtcat ctgtggaaga tgtgaaaaaa
     8521 gtattattaa caaaatcaaa acgttcaaaa atggaacagc gcggaaaatt cattagtttt
     8581 ttgtgtatcg cgctgatcgt gttggttgta ttatctattt tttattttgt cgctagtaaa
     8641 ggattggcca ccttttttac tgataagatc aatgtatttg attttctgtt tggtacagat
     8701 tggaatccaa gccaaatagg agcaaatggg aaacccatgg taggggcatt gcctatgatc
     8761 acaggttcat ttattgtgac ctttttatct gctattgttg ccacaccatt tgctattgga
     8821 gcagcagtgt tcatgacgga gatttctcct aaaaacggaa cgaagatctt gcaaccggtc
     8881 atcgagctac ttgtaggtat tccgtctgtc gtttacggat ttatcggttt atctgttatc
     8941 gtgccttttg tcagaagtat ctttggcggg acagggttcg gtatcttagc tggtacattt
     9001 gtgttattcg ttatgatctt accgactgtc acaacgatga ctgtggatgc attaaaggca
     9061 gtgccgcgac actatcggga agcttcatta gcgctaggtg ccacaagatg gcaaacaatc
     9121 tacaaagtgg tgctgcgagc agcagtgccc ggaattttaa cagcagtcgt ttttggtatg
     9181 gctcgtgcgt ttggcgaagc cttagctatc caaatggtta ttggtaatgc ggcattaatg
     9241 ccaacaggac ttactactcc tgcttctaca ttgacgtcca tcttgacaat gggaatcgga
     9301 aatacaatca tgggaacagt tgaaaataat gtgttatggt cattagcgct gatcttgcta
     9361 ttgatgtcat tattctttaa tatcgtgatc cgtatgattg ggaagaaagg agagctgaaa
     9421 taatgaacgc aaaacgctca gataaaattg ctacaggtgt tctatatgtg gtttcaggga
     9481 tcatcgtatt gatcttagca gctttattac tttatatctt agttcgagga ataccgcaca
     9541 tttcatggtc attcttgact gaaccttcca aagcctacca agaaggcggt gggatcggga
     9601 ttcaattatt taactcgatt tatttgcttt tgattaccat gatcatcagt tttcctatct
     9661 ctttaggtgc gggtatctac ctttcggaat atgccggaaa aaactggctg acagacgtga
     9721 tccgtacgtc tatcgaaatc ttgagctctt tgccttctgt tgttgtcggt ttattcggtt
     9781 tcttggtttt cgttattcaa ttccagtatg gtttctcaat catttctggt gcattagctt
     9841 tgaccgtatt caacttgcct cttttgactc gaacggtcga ggaatcttta caagcggtcc
     9901 attacacaca acgagaagct ggattggcgt taggtttgtc acgctgggaa acagtgataa
     9961 aagtggttgt tccagaagcg acaccaggaa ttttgactgg tgtgatccta agttcaggaa
    10021 gaatttttgg tgaagctgcc gcattgattt atacagctgg tcaaagcgcg ccagcattag
    10081 attttacgaa ctggaatcca ttaagtgttt ctagtccgat cagcattttc cgccaagctg
    10141 aaacactggc tgtccatatt tggaaaatca acacagaagg tacgatgcct gatggtgttt
    10201 ctgtttcggc tggcgcttct gccgtgttga tcattgctgt tttactgttc aacttcggcg
    10261 ctcgtgcgat tggcaaacga ttataccgaa aaatgacttc agcctaagga gaagaagtaa
    10321 aatgaaaaaa tataattgga atgaaacaaa tgtcattacc atgccaaaag ataaggaagt
    10381 tgcattatat acgaatgatc tgcatgtttg gtatggcgac aatgaagcaa tcaagggagt
    10441 ggatctagaa ttcgaaaaaa ataagatcac tgcattgatt ggtccctctg gctgcggaaa
    10501 atccacgtat cttcgttctt tgaatcgtat gaatgatggg attgctaata cgaaaattac
    10561 tggaaagatt atgtataaag acgtggatat caatgcgcct caagtggatg tttatgaaat
    10621 gcgtaaacgg atcggcatgg tgttccaacg ccccaaccct tttagcaaat cgatttatga
    10681 caatataact tttgctttga aacagcatgg tgaaaaaaac aaaaagaaat tagatgaaat
    10741 cgttgaaaca agcttgaaac aagcagcttt gtgggatcaa gtaaaagata gcttggataa
    10801 aagtgccttg gctttatctg gcggacaaca gcaacgtttg tgtatcgctc gtgcaatcgc
    10861 gatgaaaccc gatatcttgc tgttagacga gccggcaagt gccttggatc ctatttcgac
    10921 aggaacggtt gaagaaacac ttgtaaactt gaaagaagac tacacgatca ttatcgtgac
    10981 gcataatatg caacaagcag ctcgtatcag cgactatact gcctttttct atctaggaaa
    11041 agtaatggaa tatgacaaaa caaacaagat atttaccaga ccaaaaattc aggcaaccga
    11101 agattatgtg tccggtcatt ttggttaaga cggaggcctc aacatgacaa aagaaattat
    11161 ctcatccaaa gatcttcatc tgtattatgg aaagaaagaa gcactaaagg gaattgatct
    11221 aagtttcaat caaggtgaaa tcacagcgat gatcggtcct tctggttgcg ggaaatctac
    11281 ctatctacgt tcgttgaatc gaatgaacga tctgattcca aatgtaacga tcaccggaag
    11341 tgttttatat aaagacaaag acatctatgg tccgaaaaca gatgttgttg aactaagaaa
    11401 agaaatcggc atggtgttcc aacagccaaa tccatttcct ttttctattt atgaaaatgt
    11461 catctatgga ttgaagttaa aaggtgaaaa agataaaaaa gtccttgatc aagtagtgga
    11521 agaaagtctg aaggcagcgt ccgtttggga ggatgtgaaa gataaactgc ataaaagtgc
    11581 cttgtcttta tccggtgggc agcagcagcg tgtatgtatc gcacgtgttt tggcagtgaa
    11641 tcccgaaatc atcttattgg acgaaccaac aagtgcactt gaccctgttt ctactgggaa
    11701 aatcgaaagt atgctgttag aattaagaga gcggtatacg atgatcatcg tgacccataa
    11761 tatgtctcaa gcttcacgta tttcagataa gacggcattc tttttagatg gtcacttgat
    11821 cgaatacaac gatacgaaga agatattcat gaatccagaa aaacaagaga cagaagatta
    11881 tatttcaggt cgatttggat aaggaggaaa cggaatgcta cgtacacaat ttgaagaaga
    11941 attgctgaat ctccataatc agttctacga aatggggatg atggtgagca gcgctgtgca
    12001 caagtcagtt cgtgcataca tcaaacacga taaaatctta gctcaagaag ttatcgaaaa
    12061 cgatataaat atcaataata tggaaacgcg tttggagaag aaaagttttg aaatgattgc
    12121 attacaacag ccagttacaa ctgacttgcg tatgattatc acggtcatga aagctagctc
    12181 tgatttagaa cgtatggccg atcatgcggt gtccgttgct aaatccacaa tccgcgtaaa
    12241 aggacagacg agaataccgg aaatcgaaaa agagatctcc gatatgtccg attacgtgaa
    12301 aaaaatggtg gacaacgtcc tagttgcgta tgtcaaaaca gacgaaaaag atgcgcgtac
    12361 aatcgccaat atggatcaaa gagtcaatga ctatttcaat cgaatctata atgcaaccat
    12421 caaaaacatg caagaaaatc ctgaaacagt catcagtggc acagactatt tgaatgttgc
    12481 cagctatcta gaacgaatcg gagactatgt gacaaacatc tgtgaatgga tcgtctactt
    12541 ggctacaggg aaaatcactg aattaaatac gaaccacaat gagattttct aaactgaagt
    12601 tgtgattctg ctgtttattt aggaaaagag gttgtgaaat cagcgttttg tcctgagtat
    12661 tagagaaaca aacggtaaaa tagttcctca tattttgccg ttttttgagc taataccgaa
    12721 ggtcagctga ttgaacaccg tttattctgg aaaagggttt gtgacaaaac atgtcacaaa
    12781 cccttttcat tattccaata gaaaggataa agaatgcaaa aaaagattag catcatcatc
    12841 actctatttt ttatttgtat acttgcttca tggtttattc agcgtcaagc tgctctatat
    12901 gacgtacctg tcatcgagat cgaaagagtg agaatagaag aggatcaaca attgattaca
    12961 ggaagattga tgaatacgga ccaaaaagga gaagtgattt ctttaacggc tccttatcaa
    13021 aaaaatcaaa tcgaaacagc aaatataaca acaagacaaa tctatcttgt ccaaatgcat
    13081 ggagatgaat ggcaactagt agcaaaaaaa aatgatggct ggcttttttt ctttacaagt
    13141 gtcttcattg gcaccatgtt gcttattggt ggaaaacagg gcgtatttgc tttaggaagt
    13201 ctcatcatca acatcgtttt acttttactt ttgttgctcc tctttaccaa aacagaaggt
    13261 atttctctct taaatatagc aatcttattt gtcgttatag ggataatcgt tagtatattg
    13321 gcgttagacg gatggaatag aagcagtata atcaaaattt cggctgcagt tacaagtgtt
    13381 tttttagcat tttttatttg tcttttgaca atgaatcatt ggaaagatca aggactgcgc
    13441 tatgaagaac tgaattatct gacacgaccg tatcgttctg tttttctttc cagcgtgctg
    13501 attgggacag ctggagggac actcgatacc gtgatcacag tcattgcgac attagaagaa
    13561 ttgaccaagc aacaaccgaa aatatcagcg aaacagttat ggctttcagg aaggcaagtg
    13621 ggccgagatg tgattggttc gatggctaat atccttttat tcgtctatat cagcggagcg
    13681 attccatcgc ttctgttata tttaggaaat caatggtctt tcaaagaaac ggtagaacga
    13741 catttatcat tagaactttt acgcgtcatt gctggcagct taggaatagt attatcaatc
    13801 cccatcgcgg tcctcttttt tctcgctgta aggaggttaa aaaaatgaat gtacttatta
    13861 tacttgttgg gatttttgca atcagtgtgc tatttgtcgg tggtacgcaa ggtatgtata
    13921 ttcttttagg attattcatt aatttaggaa tctttttcct attattattc ggctatcatc
    13981 agaagtggcc gatattggta ttgtcgatta tagggttcct attgatcgct gtggttatct
    14041 tattttttat caatggttat aatctgaaaa tgcgagccgc ttttgccagt atactgatct
    14101 ttttgttttg tttcctacta ttgattccta tcactgattt tttagcgatc caaggcttta
    14161 cttcgataga actagaagaa ctatctggct tagataagac gttagcaatt gattttagac
    14221 tacttacgag gtcgttgctt ctgatcagtc ttagcggagc tgttttagat gcgagtgtag
    14281 cgatttctag tggaacattc gaagtatatc aggcaaatcc gcaaatgtct tttaaccaat
    14341 tgcgccatgc tagctttgcc attgctaaaa aaatattggc atcaacagtg atgacgcttc
    14401 tttttgcgtt tatggggagt tctttagcgt tgattctttg gtttattgac ttagacattt
    14461 cttttcagca aataatcaat gaaaaatcat ttgtcttgga atatacgatg gctattctta
    14521 ccacactggc cgcactactt gtcctgccct taacttgcgt gactacagca tatctcttta
    14581 caaaaaagaa agaacatctc aagatggagt aaaagttaca acttaagtcc tatgacattt
    14641 ttactgaatc agtgcataat atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg
    14701 aggcaatttc catgaaagaa agagaacgta ttttagactt agtgaaaaaa ggaatccttt
    14761 cgactgaaga aggcttagat ttattagaaa gcatggcaac tgaaaaagac gaaaaacaaa
    14821 tcaaaaaaga agcagacaag gtaactgcta gtcataaaga aaaagatcag gcaagtcaat
    14881 tgatcgatga acttgaaaac ggtgaagaaa aaatttctga gccagttgat ccaaaagaaa
    14941 aagaaagaca agatcaagaa aacttagaaa agatcttaga tgaactagca actgaagcga
    15001 ataaaacttc ggcgcgtcta gacgaagtaa atgctcaatt cgctgacaat aaggcagcac
    15061 gtaatgagaa acaggaagca ttgatgcagc tgaatacaaa agaagaatta ggcgagttga
    15121 cagaagaaga attagcacag cgtcaaacgt tagaagcaga aatcaaagag ttagaagcaa
    15181 ccggcgatac actattagaa gaacaattga agctggaagc agaattaaaa gatatccgca
    15241 agaatcaatg gtctgagaaa aaagaaacat ttacggataa gtttgaattg ccagatgatt
    15301 ggaaagatca agcgacagat acgctgaatc aagtaggcga aaaaatgtca gaagctggat
    15361 cacagttagg caaattcttg aagaaaacat tccaaactgt ttcagaaaca gtaaatgata
    15421 acatggaatg gaaagatgtc agcttacgcg tccctggaat tgcgacaacg aaatttgaac
    15481 acgaatttta ttatgaagct cctgcagcaa gtatccttga tatcaaggca gcaaacggaa
    15541 acgtgacgtt aaaaacgtgg gacagtgatg atgtgaaagt ggaagccaag atcaaacttt
    15601 acggaaaaat gggtgctgaa ccatttgaag cattctctga acggagccag atcgaggtca
    15661 atgaagatca tatctccttc caaattccaa ataagcgtgt gcgtgcagac cttgttttct
    15721 acttgcctaa acgagtatac gaccatgcag ctatcaaact tctaaatggc aacatcatga
    15781 ttgagacatt ggaagctaaa gatatttata caaaatctac gaatgggaac atcatcgtga
    15841 accaattaac tgcaacgatg ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa
    15901 acgggaacat cttagattct attatagaaa cagtaaacgg tacagtgaca tttggtgcca
    15961 caccagaaaa cctttctgtg tctttagtaa atggtgatgt acgtcttacg attaaagaag
    16021 ataatttgaa aaaagtagaa gcaagttcag tgaacgggaa tgtcaaagtt gctttaccag
    16081 atagcatcgg tttggaagga cacgctaaaa caagtcttgg aagcattaac agccgtctgt
    16141 ccaattacga agtagttcgt gagaaaaaag aacggacaaa ccaaatgttg caattccgtc
    16201 gtgtttcaga tgatgagatc gcacaagtgc agctttcaac gacaacagga agtatctatt
    16261 taaaagatac agataaataa agaggttgtg aaagagatgt tcagccttga gcaataagaa
    16321 gcgatttttt aaaatagctt cccctatttt aaaaaaatgt gccttattgc cgataggcta
    16381 tctcttgaac accgattaag aagaaggttg tcacaacgat tttcttcatt taaaatagta
    16441 gaacagaggt gagacatgat gaaaaaaaag ttgacaaaat ccaataaaaa tgttgttttg
    16501 acaggtactt tagcagggat tggggaatat atcgggattg atcccactgt tatccgtgtg
    16561 ctttatgtat tcttaagctt agttgctttt ggaagtccaa tcattttata cattttatta
    16621 gctttgatta tcccatctgc gcgttctgca cgaacaaact atggacataa caatgcatat
    16681 tatgaaaaaa acaattatcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16741 gatgacgaat ggagtgactt ttaatgtcgt actttcagcg tattttggtt aatacgttaa
    16801 cattcatatc gttggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    16861 ccgtccttgc cagcttcgta ttatctgtgt tgaacatgtt tgtcaaaccg attttgacga
    16921 tcctgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    16981 cgattcttaa aatgacttcc ttttttgtag gagcagaaca ttttagtttt tcttcatttg
    17041 gtgcagcgtt attcgtagct atcttgatgt cacttgtcaa tgtaattgtt agtgaacata
    17101 acagcagaaa ataaaaaaag ctgtgccgag atctcggcgc agcttttttg tttagaataa
    17161 agaatgcgtt ttctattaca ttggtaatta aataaaaacg ttttttttaa ttggtagtac
    17221 ttttggctag acaatgataa aatgatagag aacactctaa tagaaatgag gtagcatgaa
    17281 tgtcagaagt tgtaaaagtt agccaattag taaaaaaatt atctttggaa atcgtcacag
    17341 gcgacgagaa aagtttagac cgcgtcatcg tgactgggga catctcacgc ccaggactag
    17401 agttgaccgg ttattttaat tattattccc ataatcgttt acagctattt ggcagtaaag
    17461 aaatcagttt tgcggaacgg atgatcccag aagagcgttt gatcattatg agaagattat
    17521 gtagtgaaga tatgcccgca tttattattt caagaggatt gccggctccg gaagaaatga
    17581 tccaagcagc caatgaaaat gggatagctg tgctgcgttc gcctatctca acgtcccggc
    17641 tgctaggtga attatctagt tatttagatg gacgcttagc ccctcgaaca agtgtccacg
    17701 gtgtgttagt cgatgtctac ggattaggtg tactaattca aggggacagt gggataggga
    17761 aaagtgaaac ggcgttggaa ctgattaagc gcggacatcg attgatcgca gacgatcgag
    17821 tagatgtcta tcagcaagat gaactgacgg taatcggaga acctcctaaa atcctggaac
    17881 atctgattga gattcgaggg atcggtatca tagatgtaat gaatcttttc ggtgcgagtg
    17941 cggtacgagg ctctatgcaa gtccagttag ctgtttatct agaagcatgg gcgaaagata
    18001 agaaatacga ccgtctgggc tctgaagaca cgaccgttga aatcgcggaa gtgggcattc
    18061 cacaaataaa aatccctgtc aaaacaggac gaaacgttgc aatcatcatt gaagtagccg
    18121 caatgaattt ccgcgcacgt acaatgggct ttgacgcaac gaaaacattt gaagaaagat
    18181 taagtcgctt gattgaagaa aactcaggca atgactagca aggagtgaca atatgttagc
    18241 acaaatcaat cgagttgctt ttgagcttgt aggaattcct atctactggt atgcattgat
    18301 tatcgtatca ggaatcatta ttgccatgtg gctcagcagc agagaagctg ttcgggtagg
    18361 attaaaagca gatgacgtga cagattttat gttgtgggga ttgccgattt ccattattgg
    18421 tgcccgcttg tattatgttt tattcgatct tccccaatat atagctgatc caatccagat
    18481 attcaatatc cgttcaggag gattagcgat ttacggggga ttgattgcag gtgcaatcac
    18541 actgtacttt tttaccaaat atcattttat ttctttgtgg acctttttag atatcgctgc
    18601 accaagcgta ttattggcgc aggcaatcgg acgatgggga aatttcatga accacgaagc
    18661 gtatggtcca gacacgacac gaagtttctt agaaagtctg catttaccgc aattcatcat
    18721 tgataacatg tatatcgaag gaatctatcg acaaccaacc tttctttatg aatcactttg
    18781 gagtttgatt ggatttattc tgcttttggt tttgcggaag aaaccacatc ttttgaaaaa
    18841 aggagaagtc cttttatgtt atctaatgtg gtatagtttc ggacgtttct ttatcgaagg
    18901 gttacgcaca gacagccttt actttttagg tgtgatccgc atttctcaat tattgtctgc
    18961 tgttttattt gtcggcacat tgattctgtt tatttggcgt agaaaaaaag gaagtttacc
    19021 ggattatgac cgctcattag gaaaaaatca acaaattatt tgattttcag agcgttaaaa
    19081 atgaaaaaaa tttcaggagt ggtaaaatga attatagagt agcggtttta ggaccaggct
    19141 cttgggggac tgctttagca caagtcctcg ctgaaaacgg acatgacgtt cggatttggg
    19201 gacatcgtgc ttcacaagta gaagaaatca acacccaaca caccaatcaa cgttatttac
    19261 cagatcatca tctacccgat tccatccgag cattcaccga tatggaagat gcagtaaaag
    19321 atgcagatgc ggtactattc gttatcccga cgaaagcgat acgttctgtt gcaaaagaac
    19381 tagttgccaa attgaatacg aagccagtga tcatccacgc aagtaaggga ttagaacagg
    19441 gaacgcataa aagaatctct gaaatcttgt tagaggaaat cccgacagaa aaaagaaaag
    19501 ctgttgctgt tctttctggg ccaagccatg cggaagaagt agcaaagcat gacattacga
    19561 cgatcacagc ggcaagcaca aatgtaaaag cagcaaaata tgtacaagag ctgttcatga
    19621 accattattt tcgaatctat accaaccctg atgtgattgg tgtagagatg ggagcagctt
    19681 tgaaaaatat tattgccatt ggagctggtg ctattcacgg cttagggttt ggcgataatg
    19741 caaaagcggc aatcatgacg cgcggattag ctgagatcag ccgtttaggt gtagcaatgg
    19801 gtgccaaccc gctgaccttt attggcttaa gcggagtagg tgatctagtc gttacttgca
    19861 caagtgttca ttccagaaac tggcgcgcag gcaacttgtt aggacaaggt cagcctctag
    19921 aagaggtgct agccaatatg ggaatggttg ttgaaggtgt gaatactact caagcagcga
    19981 tggaactagc ccaaatgatg gaaatcgaaa tgcccatcac tcaaacaatc tatgaagtgc
    20041 tgtatgaaca aaaagaaatc aaaacagccg caaaagaaat catgttacgt gatgggaaaa
    20101 aagaaaatga attctcatta acaatttttt gaaggagcga atgtatatga aagtaaaaaa
    20161 agcagttatt ccagcagcag gattaggaac aagattttta ccagcaacaa aggcgatggc
    20221 aaaggaaatg ttgccaatcg tggacaaacc aacgatccaa ttcatcgttg aagaagcgtt
    20281 gaaatcagga atcgaagaca tcttgatcgt tacaggaaaa gctaaacgac caatcgaaga
    20341 tcactttgat tcaaatatcg aattagaaac aaacctaagt gaaaaaggaa aaaccgaatt
    20401 attgaaatta gtagaagaaa cgacagatgt caatttgcat ttcatccgtc aatctcatcc
    20461 aaaggggtta ggacacgccg ttcttcaagc aagagccttt attggaaatg aaccgtttgt
    20521 cgttatgcta ggcgatgata tcatggaaga cgaagtacca ttgacacagc aattgatcga
    20581 tgactatgag cgtacacacg cttctacgat tgccgttatg caagttcctc acgatgaaac
    20641 ttcaaaatac ggaatcatca acccagggga agtactggaa gatgggttat acaatgtcaa
    20701 aaactttgtt gaaaaaccag atccaagcaa agcaccaagt gatttagcta ttattgggcg
    20761 ttacttattg actccagaaa tctttgatgt attatcagaa caacaaccag gcgctggaaa
    20821 tgagatccaa ctaactgacg caatcgatac gttgaataaa acacaacgcg tatttgcccg
    20881 taaattcaca ggtaaacgtt atgacgttgg tgacaaattt ggttttatga aaacaagtat
    20941 tgaatatggt ctgacacatc cagaagtagg ggcacctttg cgtgaataca tcatgtcttt
    21001 aggtaaaaca ttagaagcag aaggaagcaa gatcaatcat aatccaaaag gaataacaga
    21061 aatcaaagaa gaaaagaaaa aataatcttt ttttctaacg agcttcagct gctaattgaa
    21121 taaaaagtag ttgaagctcg tttttatttg tttaaaaaac acgaaaatgc tatccttata
    21181 gaaaagagag acaagaaggg agtttgatca tgtcaggagg agaaattgca ggattgatcg
    21241 cagcaatcgc ctttgcgata cttgtcgttt tcatcgttcg cgtattgttg caagtttcca
    21301 aaactttaga aaaagtagac cagactgtcg cagaagcaaa tacgacaatt gagatcgtca
    21361 cacaagacgt agatctttta tctagacaag tagaaggctt gctagtcaaa agtaatgagc
    21421 tgctcttgga tatcaatgga aaagtcgcaa cgatcgatcc attgttcaca gcagttgctg
    21481 atttaagtga aagtgtatcg gaacttaatc attctagtaa aaacatcgtg acgaaagtcg
    21541 gcggaatcgg aaaaacgaca gctaaagcga ctgttgccgg aaaagtcggt ggaacagcga
    21601 tgaaattttt aaaaacaaaa aacatactga aacgacagga ggaaaataag aatggctaaa
    21661 aaaggtggat ttttattagg tgcagtagtt ggaggtacag ccgcagcaat cgccgcatta
    21721 ttgttagcac ccaaatctgg taaagaactt cgggatgaat tggctgctca agcagatgat
    21781 ttgatggaca tggcttcaga ttacacagat atggcaaaag aaaaatcatc tgagttaaca
    21841 ggtattgcaa aagaaaaagc gcaagatctt tctcaacaag caggggatct agcaggcaat
    21901 gttaagcaga aggtaagtga atcagcagag gaagcaggaa gcgaatcaga tgatatcttg
    21961 ttaaacctca aagaacaatc agctgattta tctaatcgtt tcaaaaaaac agcagaagat
    22021 ctgaaaaagc cagcagacga attaggtagt attgctaaag atacttcaga agatatcatg
    22081 atcgatgtaa aagatgctgc agataaagca ggagagacgg tcaatgaagg aatgtcagaa
    22141 gcaaaaccag ttgcagatga cacaaaagaa atgttgaaaa tggcaaaaga agatgcaaaa
    22201 gaagatgcaa aagaagctaa aaaagaaatg aaagatacaa tgacagattc gactaccaat
    22261 cactaagatt actcatttct agaataaaaa aggtgagagc ggaccagctc tcaccttttt
    22321 tattttcatt actctaaaac aagtaattct tttggtgtag tcgtgaatgg cacacaacca
    22381 tcacttgtca catacacaca atcttcgata cgtaccccta ctttttcagg aatatagatt
    22441 cctggttcaa gagagaagca catcccttct tcaatcacta agtcgtttcc ttctacaaga
    22501 gaagggaatt cgtggacggt tgttccgatt ccgtgaccta aacggtgatt gaaatattcg
    22561 ccgtagccat agctgttgat cacgtttcgt gcgatttgat caagttcacc tgctgtcaca
    22621 cctgggcgaa ctgcctcttg tgcggcaagc tgtgcttcaa gagtgatgtt atagattttt
    22681 tcttggaatt cagttggttt ttgataagca actgtacgag tagcatcact gcaatagcca
    22741 ttccaaataa cgccaagatc gaataacact agttcatttg gtgaaacctt cgtgcttcct
    22801 ggtgtaccat gagggcttgc agcatttgct cctgcaagga ctaatgtgtc gaaagacatt
    22861 tgtgaaacac cttgtttttt taattggtat tcgatttctg cgataattgc ttgttcagtt
    22921 acgccttctt ttactgtttt gaagccaatt tcaaaggcaa catcggccca tttaccagct
    22981 tcgatcagtt tgtctttttc ttctaccgtt ttgatcagtt gcagacgctg gataatagaa
    23041 gttagatcgg tgtaagaatc tgtacctgtc aagatatcag agatagcatc gaagcgatcg
    23101 acagtcagag cattcttttc aatacctaca ttaccaggtt gcccatagcg tgcgcgaagc
    23161 tcttttccga tgatctccca aggattttcg ctgtctaagt agccacgtac atcgtacgcc
    23221 cagctgctgt tttgagcatc ttcaacttcc agagcaggag tgaaaaggaa aggatcttgt
    23281 tccagaggga taaaaagagc tagtacacgc tcgtgaggat cactttcata tccggaaaag
    23341 taagcgatgt ggccagggtc gctgatataa gccaattcta attggtgttg tgccatccat
    23401 tgacgtagtt cattgatttt tacgttattc atttggcaac ctactttcta aattaatttt
    23461 gtattcaata attgtaccac tgattgatta aaagaccaac gttcttaaaa ctgaaaaaag
    23521 taaaaatttt catgaaaaca gagataaaac ggttgcaatt gttgagatta tttgatatcc
    23581 tattttagaa atgtaagacg tattttcaga aaacatataa aatatgaata ggagaacaca
    23641 aatggaaaag caaacaatca ctatctatga tgtagcgaga gaggcaaatg tttcgatggc
    23701 tactgtttca cgtgtcgtaa atggcaatcc aaacgtcaaa ccagcaacaa gaaaaaaagt
    23761 cttggaagtt atcgatcgct tggattatcg tccaaatgca gtcgcacgtg gcttggcaag
    23821 taagaaaaca acaacagttg gggtaatcat tcctgatgtc agcaatatgt tctttgcctc
    23881 tttagcacgt ggtatcgacg atgtggcaac aatgtacaaa tacaatatca tcttagcaaa
    23941 ctcagatggc aacgatcaaa aagaagtcaa tgtattgaat aatttattgg ctaagcaagt
    24001 ggacggcgtg atctttatgg gtcaccatat cacagacgaa atccgtggag aattttcacg
    24061 ttcaaaaacg cctgttgtac tagctggttc cattgatcca gatgaacaag taggtagcgt
    24121 aaacattgat tatgtatcag caacaaaaga tgcagtaaat aaattagcaa aaagcggcaa
    24181 taaaaaaatc gcttttgtca gcggtgcgct gattgatcct atcaatggtc aaaaccgtct
    24241 aaaaggctat aaagaagcat taagcgaaaa caatctacca tatagcgaag gattgatttt
    24301 tgaggcacaa tacaacttta aagatggtct gtctctagtt gaccggatcc ataatagcgg
    24361 tgcaacagct gtctatgttt cagacgatga attagccatt ggtatcttgg atggtttatt
    24421 agaccgcggc gtaaaagtac cagaagaatt cgaaatcatc acaagcaaca attctttact
    24481 gacagaagta gctcgtcctc gtttgacaag tatcacacaa ccattatacg atttaggagc
    24541 ggtatccatg cgcctattga cgaaaatgat gaataaagaa gaagtcgaag aaaaaacgat
    24601 tatcttgcct tatggaatcg aagaaaaagg atcaactaaa taataaaaga tcaatcattt
    24661 aaaggttgtt cccaaagtgg tcaacttctg gaacaacctt tatacgagaa aaggggttat
    24721 gacaaaattt tgtcataacc cctttttttg tgactttaaa aaaaagagag aatggcggaa
    24781 acaaacctta cgtccatcgt atacgacaca atctgtgcgt agatgctaca ttttgttcac
    24841 tactatcaaa attactttaa aatgaaaacg tgataaaaaa gaaagtagaa aagtagaaaa
    24901 gtaactctaa aaaatccaaa aaaatccagg ctcgattgcg tacttatctt aaaggagaaa
    24961 caatccatca tacataaccc gaaaggagac ttctatttgc ctaagaaaag acgaaagacc
    25021 atagtcatca ttttgaccat tcttactata ggaatcggag gaaaatttta catggacaaa
    25081 agagaagcgc aaaaacagca agatttactc gcagtagaaa aacagtccgt caaagttctg
    25141 aaaaatacct ttgcggatat caaagaagtg aaaatcgaac aatttgagaa aaacgacatg
    25201 acaggaatct atgaagcatt gataacgatg accaatacag aaggaaaagc cgtttatttt
    25261 gattatagct ttacaaaaga aagcaatgag atagatggct atggtgttga aaatgaagaa
    25321 gtacaaaaag aagggattac ggttgatgag attatagtta tctatacaga tgggagtgag
    25381 gaaaaagtat gacaattact gataagcagt ataaaggaat aagtaataaa gtatattggc
    25441 tagatccaaa acatcctcaa tatagtaaaa gttacaaaga aggcgccatt aaaaactttg
    25501 gagaaacaaa attccaaatc ctaaaaatca aaaactcctt cgacggcatg caggcgatgg
    25561 ctgtagcgcc aatcaagaac ggtaaagtgg atatttctca agtagtgatt gcttttgcag
    25621 gaacgaactt aagcgattgg aaagatttgg aaacggatgc aagaagtgtt atgaatctct
    25681 atgggaatag accaggagca agtttttctg aaaaagcaac aacaaacaag attttatccg
    25741 ttagtggatt gaaaacagcg gggcaaatca attcagcgat tgcatttgct aatgaagtaa
    25801 aaaaggaata tggagaagct gctatttcta ctaccggcca ttcattaggt ggattcttag
    25861 cattatatgt agcagcagag aaacaatgga aaaatgtcgg cttcaacggg ccggacccgt
    25921 atgatctctt atcaccagaa gcaaaaaaat gggtggaaga aaatcccggt atgctcacca
    25981 attaccgaaa tcacgccgat tttatcggta attttggtgg taatggcaca ggtgctgaag
    26041 ttcggatcag tatggaaaca gcatacttga atctgcttgc ttcccacagt ttaacctctt
    26101 ggaagtttga tgaaaatgga aagctgatta ttcctgaaaa tgaaaacaat gttaaagctc
    26161 gaaaacaaca gaaagaaaac caagcgaccc agttgttttc ctatcagttg caagaactga
    26221 acaacatacg taaaaaacta cgattaagtg gcggccggct gacagttaat gaacaaattt
    26281 acttagatga cacacaagct aggttagtgg tggaaaagtt atcggtaaaa atgaaaagcg
    26341 ctatggaaga agttatccgc atcaaccaaa gaggaatttt tgagctagag aaaaaatggc
    26401 aagaaggctt gcggcaagca cgtgttgtcg ccccttcgtt gactgaacag gaaatcatag
    26461 aagcattagc tagtgtgggc gccacaaaag agaacttagt cgattctgcg aaagaagtct
    26521 atctgctacg aatcaataag gcaagacgta tgggggaggc ctttgataca ttggcgaagg
    26581 agatccaaga caaaatcaat gaaatcgtgg cacgagatcg agaattagca cagcaattca
    26641 attaaaggag aagtagatga aactagaaga attggaaaga gattatcgaa gtgaacgaag
    26701 acaattagaa gctcaagaag atgaattgat ccactttaag aatcaaggat tacgattgat
    26761 ggagcaagag gagcagcgac tgcatcatgt attaaagaac ttagctttag ataatgagcc
    26821 tctgtttcaa gcaagacgaa gttattcgaa ttatcaggag cattatcaac aagccctaca
    26881 agtagaacga cgaaaactct atcagcagca agacgaacta gaacgttcct atcgtcaaaa
    26941 aatcagacaa ataactatgg agaaatcatc ttatgaatga atttttaatg caattactaa
    27001 aagaattaga gaaaaatcta catcgatcaa atgtacgttc agttggtatg gggatatctc
    27061 agcaaacttg ggcagaaaaa cgattgacag atcaaaacca ccaaatgact gcagaagaag
    27121 ctactcaaga attttctaac cgacatgcac acttaaaaga atcagtaaaa gggcaatttg
    27181 cagaaaaact aaccgaatta ttccaaagac aagaaaagaa attaaaacag tttcgttagt
    27241 aaagttatat agaaaaattt tgtgaaaatg gtttttcaca tttttacata aatttgtctt
    27301 aatatcgtag atgtaagctt ttgaacacca tttattcgga aaaagggagc tgtgacaaga
    27361 tttttgtcgc agctcccttt aattctgatt aatctattag atcaatcatc tttatcgtct
    27421 gattctttgg tctctggatt cgctctagca taagctgaag cggtgttcca gtaatctgta
    27481 tagttttcat cagtaccgcc aacgccgaat ttaaaggcgc ttttcttcgg accatttttt
    27541 gcccaaagtg attccactgt ttttcctggc gtttggatag attttttatt gacagtaact
    27601 tttccagctg tttgtcctgt gaacccagat acttttttct tcatcacatc ggatgataag
    27661 tcaaatttct catcagtgcc gaaaatctca gggtttgctt ggtaagcttg attgattaga
    27721 ttggcaagat aattagccga acgtattctt gcttggctgt ccattccagt attgtcatcg
    27781 tgtcctgccc aactactgat agtgatagat ggagttgaga cgatcagcca tgaatctcgg
    27841 tattcattgg tagaaccagt ttttccgacc caatctgcat ttcctagatt tccgtttaag
    27901 cttgagatga catctttgaa tggtgtagtg attttttcat tgatcacact acgcatcatg
    27961 tcattcatga tcgatgccgt tgcttcggaa taaatacgga cagaattact ttcatgttta
    28021 taaatcacat tacctgcatt atctgtgatg gaatcaatga tatagccttc ttcgtagact
    28081 ccaccatttg ctaaggcctg gaatccattt gtctgttgaa gcgtcgttac atctactgtc
    28141 cctaaaggag ccgattcaac gccccaatta tcatttgctg ggtagttcat cttcgccaag
    28201 tagtgctcgt aagcgtattg tttcgatcca ccggaattca acacatcttg atataagtga
    28261 taagctggga tattgttgga ccattccagt gactcacgga cggtttggaa ggtgttcgac
    28321 ccttcattgg tcgcattgac gattttctca ccagcatttt ttccttcttg ccaagtcgtt
    28381 gcataatctg atacacggga ttctgatccg acaagtccca tatcgattgc aggaccgtaa
    28441 acaagaaccg gtttgatgga tgaaccagct tgacgttctg tatcaaaggc gtggttattt
    28501 tgattctggc tgtaattccg accacccacg aagccataga tacgtccggt tctgttatcc
    28561 atcaatacat ttccagtctc cacttggcta ccagcaccat catctaacat gtagccataa
    28621 ttttctacgc cggtttgcaa tgcagcatag atatctttat caatcgtcga atggatcgta
    28681 tatcctttgt tttggatcgt ttgctgtgct ttttgataat aagcttcact gaccgatgaa
    28741 tctgacatat cagcattatc tttttctgcc aattgttgtg caataatcct tgttgcttcg
    28801 ttcatcacgg tataatacaa gaattctctg tcagtctgtt ccgcgatttg ttgtgggaga
    28861 aagtcttttg taagatcata ggctttcgct tcttcgtatt gttctttcgt aatttgtcct
    28921 tcacggtaca tactgaataa aacaaaatct tttctggcaa gaccggcaga aagatcttct
    28981 tttagcgcgc ctgtattcgt gtatggacta tacgtgatag ggctttgagg caaaccagcg
    29041 atataagcag cttgcggcag agtcacatcc tttgcatcta caccaaagat tcctctagca
    29101 gcttcctgga ctccagcaat attttgcccc ttattattcc gaccaaatgg tgagacattc
    29161 aaatacgtag taatgatctc atcttttgaa aagtgttttt ctacttgtgc agaaagaacg
    29221 atctcgtttg ctttacgttt gaatgttgtt tcatcggtta ggatctgttg tttgactaat
    29281 tgctgtgtca aagtggatcc tccagacgag ccgatgccag tcgcttctga aactagtgct
    29341 cgtaaaacag ctttagggac ataaccttgg tgtttatcga agtattcatc ttctgtactg
    29401 ataatcgcgg ttttcaataa aggcgaaatc ttatcagatg aaatagtcgt ccgcatcaga
    29461 tctgtctgga tttttgatat ctctgtatta tctgcataaa caatctttga cgtttccgta
    29521 atgtttccta gttctgtctg cagttctttt ttagtcggca attgtgtgtc ttcgacaaga
    29581 taggcaaagt agccagcacc tattccgata cctagagctg cgaacaacaa aagaagaaca
    29641 gtaataaaaa cagtcaatga ttgaaacact ctaaaaataa tcttaggaac aaaccatttt
    29701 tctttttttc ttctattctg tttttttcga gtatttttgc gtggcaaaag ggtcacctca
    29761 actaatggtt ttatttcgct aattatagca aataatagga caaaaaaaca gacaaggctg
    29821 tttttgacag gaatttcttt ttcgaatgag ataaaacgtg aggaaatgat cttttttatg
    29881 aaagagttct taagcttttc tttagggaaa gtttcagtta ctatggagaa cagggcaaag
    29941 aaaaacaaac tattttcttt gtacatcatc ttatcaatat acaggaattg ttcgaaacaa
    30001 ttgcctttca aggaggaatc catccatgtt attaggctca cacgtcagca tgagcggaaa
    30061 gaaaatgctg ttaggcgcgg cagaagaagc agccagttac ggatcttcga cctttatgat
    30121 ctatacaggc gctcctcaaa atactagaag aaaagctatc gaagagatga atattgatgc
    30181 cggaaaagca tttatggcag aaaacggact aacaaatatc gtcgttcatg cgccttatat
    30241 cattaatttg ggaaatacga tcaaaccaga aatgtttcca tttgctgtac aatttctgag
    30301 agaagagatt gtccgtgcgg aagctttagg tgctactcaa atcacccttc acccaggcgc
    30361 tcatgtagga gcaggggcag aagctgggat tgctagaatt attgaggggt tgaatgaagt
    30421 tttgacaaaa gaacagacgg cacagatcgc tttggagacg atggcaggga aaggtacgga
    30481 aatcggtcga acatttgagg aattagcgca aatcatcgat ggagtaaaat tgaatgaaaa
    30541 attatccgtt acttttgata catgtcatac gaatgatgcc ggatataata taaaagaaga
    30601 tttttcaggt gtaatggaag agtttgataa ggtcatcgga ttagatcggc ttaaagtact
    30661 gcatatcaat gattctaaaa atcctcaagg ttctcataaa gatcggcatg ccaatatcgg
    30721 ttttggaaca attggatttg acacattgaa caaaatcgtc catgatccaa tgtttgacaa
    30781 catttcgaaa atattagaaa ccccatatgt cggtaaagat aaaaagaaag cttacgcacc
    30841 atatggaaaa gaaatttcca tgttcaaagc gcaagcattc gatcccgaag tgttccctga
    30901 gttattagca gaagaagtca cactttaagt tttctacctg acagcaacaa aaaaggattg
    30961 ggcagtctgc cttgtccttt tttgtttaag aaacaaagga gtgctttttg cattcgtttt
    31021 cgccatgctc cttttgcccc ctctttttaa attcagacaa acagggtata ctgatagaaa
    31081 agtaaaaaac gaaaagtttc acaaacaagt agcaaaacgt gattgaaagg acgaaaagat
    31141 gctgaacatt ttaaacccct tgatcgacca actaaaaaag aaacatacat actttattct
    31201 tggcatagcc gtattattta caattattcg gttgtattta tatagtaaag caatctatgg
    31261 aacagatatg tcggcgacct atgatgatca gctgctgatt cactatgctc aaaatatttt
    31321 ggatggtaaa tggctagggg agtacacgac tacgacactt tcaaaaggga tttcctacag
    31381 cttgttttta gtacttgcac ataaactgca tctttcttat gcagttttat tcggcatgtt
    31441 cagtattctt gccagtatat tggttgcact agcgtttaaa ccaatcgtga agaacaagta
    31501 tcttctttta agtatctatt tttatttttt gttttctccg attagtttta cacatgaata
    31561 ttccactcga acatatcgta atgctctcgt gatcccgact gttgagatca ttatcgcttg
    31621 tttactagct atttattacc gcaaatataa ttccagcaaa aaattgctgc cgtggttttt
    31681 agggctagct gtttcttttc ctttcttttg gttcattcgc gaagattcca tctggcttct
    31741 tccttttact gtagtcgcgt taggcatcag tttcgttcag atcttgctag gacaagactt
    31801 ttctttcaaa gaagcaaaga agttaaaaga acgaccattt ctcctttcga aacaagaatg
    31861 gctgaaactt tcattgtttt tactccctct agtcggtttc tccatcgtct atcagtggat
    31921 cgaatataaa aacgagacaa catatggtat tgcagccatc aatgaccgtt cgagtggcga
    31981 atttggacgt ttgatgaaac agctgatccg aatagatgac gaaacaaact taaataaaga
    32041 aaacagtgag atatgggtct cgcatgctgc attaaataaa gctttagacg catctcctac
    32101 atttcatact gtttctactc gtattgacga attgtatacg actcatgcgt ggacccaagg
    32161 cggaaaagta aaagaattac caggagatat cattttttgg gcattgcgag atgtggtaag
    32221 cgaatctggt tattaccaaa acaatgcaaa agaaaccaat gatttttgga aaaaagtcaa
    32281 tcaagaatta gaagaagcct atcaaaaggg aactatcaaa aagaaaaaag aattctatct
    32341 gatgggatca ggggatggaa agacgatgaa tgatctgcca atcgtatttg attttctgaa
    32401 atcaggatat gcttatgcat ggcgatacaa agatttccat caagggggga attatagttt
    32461 tgggacgcaa gaacaagtcg aacaggcaca agaattattg aatcttccac taatggataa
    32521 ttggatcgat agcagtgatc cgagaacacc tgatttccag ttgacaaaaa cagcaaaagt
    32581 cgcgaatgtc atgataaaaa tttaccaagt tctggccatt cccgtgctga ttgcttcaat
    32641 agcagggata ttacttatga tactaggaac cgtatttgca aaagctgatc gtgcctatta
    32701 tagttcctct ttgattgtag tcatcggatt aatactgaca caattggttt ttcttttcgg
    32761 tgtctcgtgg ttttgttctt atgcgccaga aaaaaaggat tattttttaa gtgtctacac
    32821 tggcgcaggc gtgccattaa cgcaattcat cgcagtaaca agcatgctag tttttagtaa
    32881 attgaatctg aaaaaagctt tatcttccaa ataaaaaggg aactgtgaca aaagtcttgt
    32941 cgcagttcct ttttccgaat aaacggtg
//
LOCUS       DACDYJ010000026        31521 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDYJ010000026 DACDYJ010000000
VERSION     DACDYJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.026"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            189..557
                     /locus_tag="IWM51_001261"
     CDS             189..557
                     /locus_tag="IWM51_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9970690.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            737..1678
                     /locus_tag="IWM51_001262"
     CDS             737..1678
                     /locus_tag="IWM51_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP9970691.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1747..2478
                     /locus_tag="IWM51_001263"
     CDS             1747..2478
                     /locus_tag="IWM51_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP9970692.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2622..4802
                     /locus_tag="IWM51_001264"
     CDS             2622..4802
                     /locus_tag="IWM51_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP9970693.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4822..5304
                     /locus_tag="IWM51_001265"
     CDS             4822..5304
                     /locus_tag="IWM51_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP9970694.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5320..6081
                     /locus_tag="IWM51_001266"
     CDS             5320..6081
                     /locus_tag="IWM51_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9970695.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6212..7546
                     /locus_tag="IWM51_001267"
     CDS             6212..7546
                     /locus_tag="IWM51_001267"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP9970696.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7853..8338
                     /locus_tag="IWM51_001268"
     CDS             7853..8338
                     /locus_tag="IWM51_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP9970697.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8331..9014
                     /locus_tag="IWM51_001269"
     CDS             8331..9014
                     /locus_tag="IWM51_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP9970698.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9140..11785
                     /gene="polA"
                     /locus_tag="IWM51_001270"
     CDS             9140..11785
                     /gene="polA"
                     /locus_tag="IWM51_001270"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP9970699.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11830..12666
                     /gene="mutM"
                     /locus_tag="IWM51_001271"
     CDS             11830..12666
                     /gene="mutM"
                     /locus_tag="IWM51_001271"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP9970700.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12630..13259
                     /locus_tag="IWM51_001272"
     CDS             12630..13259
                     /locus_tag="IWM51_001272"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP9970701.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13311..13643
                     /locus_tag="IWM51_001273"
     CDS             13311..13643
                     /locus_tag="IWM51_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9970702.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13955..14446
                     /gene="nrdR"
                     /locus_tag="IWM51_001274"
     CDS             13955..14446
                     /gene="nrdR"
                     /locus_tag="IWM51_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP9970703.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14469..15842
                     /locus_tag="IWM51_001275"
     CDS             14469..15842
                     /locus_tag="IWM51_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP9970704.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15842..16768
                     /gene="dnaI"
                     /locus_tag="IWM51_001276"
     CDS             15842..16768
                     /gene="dnaI"
                     /locus_tag="IWM51_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP9970705.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16897..17337
                     /locus_tag="IWM51_001277"
     CDS             16897..17337
                     /locus_tag="IWM51_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9970706.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17481..18182
                     /locus_tag="IWM51_001278"
     CDS             17481..18182
                     /locus_tag="IWM51_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP9970707.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18179..19300
                     /locus_tag="IWM51_001279"
     CDS             18179..19300
                     /locus_tag="IWM51_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP9970708.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19315..20547
                     /gene="pepT"
                     /locus_tag="IWM51_001280"
     CDS             19315..20547
                     /gene="pepT"
                     /locus_tag="IWM51_001280"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP9970709.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20679..21587
                     /locus_tag="IWM51_001281"
     CDS             20679..21587
                     /locus_tag="IWM51_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP9970710.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21622..21894
                     /locus_tag="IWM51_001282"
     CDS             21622..21894
                     /locus_tag="IWM51_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP9970711.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21905..22951
                     /locus_tag="IWM51_001283"
     CDS             21905..22951
                     /locus_tag="IWM51_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP9970712.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23202..25811
                     /gene="clpB"
                     /locus_tag="IWM51_001284"
     CDS             23202..25811
                     /gene="clpB"
                     /locus_tag="IWM51_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP9970713.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25962..26633)
                     /locus_tag="IWM51_001285"
     CDS             complement(25962..26633)
                     /locus_tag="IWM51_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970714.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(26626..27120)
                     /locus_tag="IWM51_001286"
     CDS             complement(26626..27120)
                     /locus_tag="IWM51_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9970715.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(27140..28360)
                     /locus_tag="IWM51_001287"
     CDS             complement(27140..28360)
                     /locus_tag="IWM51_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9970716.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28507..30342
                     /gene="lepA"
                     /locus_tag="IWM51_001288"
     CDS             28507..30342
                     /gene="lepA"
                     /locus_tag="IWM51_001288"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP9970717.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(30436..31518)
                     /locus_tag="IWM51_001289"
     CDS             complement(30436..31518)
                     /locus_tag="IWM51_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9970718.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        10504 a         5627 c         6748 g         8642 t
ORIGIN      
        1 tataaataca cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt
       61 atttattatt aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat
      121 tttcccattt tcctatggta aactgacaaa gttgagaatg ttagaacaga tcgtttagga
      181 ggaaacagat gtttacgaat gaaataaaaa tcatgcttta tgtggacgat gtagaaaaaa
      241 atgcaacgtt ttggagagca gttggctttg cggaaaaaga tcgtcaagaa atggatggga
      301 cgcttataat tgaagtagga gttgaggaca gtcaaacctc atttctgtta tatgatcgag
      361 catttgttga gtcccattca cctggcacag ctactagtgc cccatctttg atgttttaca
      421 gtaaagatat ctttggacta tataaaaaaa tgcagacgca atcagtcaca ttaggtgata
      481 tggtacagct tggagaagaa tatgtcttta actttgctga tcccgatggc aactactttg
      541 cggttacagg taaataatag aaataaagac tttgtttttc tgtgcgaaac aggaaagcga
      601 agtcttttta attcaaataa taatattatg taaacaaaaa actttccatg cgtaattata
      661 cacaagcaaa taaatcgtca aatgcgaacg ccttctttat actgtttagg aacgaaaaga
      721 aggaaggaag aaatacatgg attatcaagt actgctttat tacaattata caaaaatcga
      781 agaaccagag gaatttgcca aaaatcattt agccttttgc aaatctttgg atcttaaagg
      841 tcgtattcta gtagctgaag aaggaataaa tggtactgtg tctggaaccg ttgaaaacac
      901 gactgcctat atgaaagcaa tgaaagctga tcctcgattt gctgatacga tgttcaagat
      961 cgatccaagc gaaggacatg cctttaagaa gatgtttgta agataccgtc cagagcttgt
     1021 aagtttaaag ctagaagagg acattgatcc aaaccaagtg acaggtgcct atctttcacc
     1081 gcaagaattt aaagaagcta ttttagatga acagacagtg gtgattgatg cacggaatga
     1141 ttatgaatat gacttgggac attttcgagg ggctgtacgc ccagatatac gcagttttcg
     1201 agagcttcca gactggatcc gcgagcacaa agaagaattt atggaaaaac gggtagtgac
     1261 ctactgtact ggtggcattc gttgcgagaa gttttctggt tggcttgtcc gtgaaggatt
     1321 caaagatgtc ggacagctgc atggcggtat tgctacttat ggtaaggatc ctgaagtcca
     1381 aggagaatta tgggacggca agatgtatgt gtttgatgaa cggatcgctg tcgatatcaa
     1441 ccaagtagat aaacaaatcg tcggtcgcga ctggtttgac ggaacaccat gtgaacgata
     1501 tatcaattgt agcaatcctg agtgcaatcg tcagattctt gcttccattg aaaatgaaga
     1561 aaaacattta ggagcatgta gcatagagtg tgcgaaacat ccgcgcaatc gctatgttca
     1621 agaccatgga atctctccag aagaagcaga actgcggttt acagctttaa ttaaatagaa
     1681 aacaatagtt ttgattgtcg accatttcca atatgcgcta tactagcgtt gaggatggtg
     1741 atccacatga aatcattgat ttatgctatt ggggatgtcc atggcaaaca tgagcttttt
     1801 gaaaaactta ttcactatta tgatccaaag atccatcagc ttgttttatt aggggatctc
     1861 aatgatcgag gtcctcgtac aaaagaatgt tttttattag gaaagcaatt agtcgaagaa
     1921 acaggagcca tttacctccg gggaaaccat gaagaatatt ttttgcaatt tctgcaatca
     1981 ccggaggact ggtatgcgcc ttatatgaga aatggtggaa aagaaacgtt tgaaagcctg
     2041 attcacccag gggcatcgga ggaatactcc ccaactgaaa tctcgatgat gatccaatcg
     2101 agatattcag aactgattaa atttttagtc cagcgcccct tgtattatga atgggaaaat
     2161 tatctttttg tccatgccgg tgtggatttg acaaagaaag actggcgtaa gacaagtcta
     2221 agagacttta tttggataag ggaaccattt catgaaggga aaaacaatac aggtaagaca
     2281 attgtatttg gacatacgat tacaccaatg ctccatggag atatgcagac aacagacctc
     2341 tggattcagg atcataaaat cggaatggac ggtggggctg tattcggagg gtcattgcat
     2401 ggggttgttt ttgacaagga aggtattctt caagatattg aatatcagaa caacgaagga
     2461 ccttggcatg gaatctgact aatggaaaga atgaaggagc tgtgactttg ttgcggctct
     2521 ttttattttt atagagaata ttgtttcttt cagtagttta acccaggata aaaagtatga
     2581 tagaataaag aagcaatttt tagcagaaaa gaggcatttt aatgacggaa aatttgaacc
     2641 agacgatcat tcagcttgta caaaaagaac tttcagacta tcgtcctaag cagttgacta
     2701 ctgtacttaa tttgttaaat gaaggaaaca ctgttccttt tatcgcgcgt taccgaaaag
     2761 aaatgactgg aagtcttgat gaagtccaga ttcgtgaaat cgaagagcgg tatgcctatc
     2821 ttgaaaactt agaaaaacgt aaaactgaag tgattcggct aatcgatgaa caagggaaac
     2881 tgactcccga gttagaagca gaaatcaccc aagccgtgaa aatgcaacaa gtggaagatt
     2941 tgtatcgtcc atacaaacaa aaacgacgga caaaagcaac gattgctaaa gaaaaaggac
     3001 ttgaaccact cgcgaagtgg ctgatgcagc ttacagatgg agaggtgcaa tctgaagcag
     3061 aaaaatatat tgacaaagaa aaagaagtct cttctgccga agaagcgttg cacggtgctc
     3121 atgagattat tgctgaacaa gtcagcgata atgcaaaatt tcggacttgg attcggtctt
     3181 atacgtataa caaaggtatg tatgtcagca atgtcaagga tgagcaagca gatgaaaaag
     3241 gcgtatatga aatgtattat gactttgcag aacccgtcca taaaatggtt tctcatcgaa
     3301 tattagctac taatcgtgga gaaaaagaag atattctgaa agtattcttt caagtagatg
     3361 aagcagcaat tttagcttac ttggatcgcc aaatagtcaa aaatcctgcc tctccttctt
     3421 cttcattcgt cagagaagcc tatcaggata gttataaacg ctttattcag ccagcaatcg
     3481 agcgagaact gcgcaatgaa ttgactgaaa aggcagatga gcaagcaatc gctattttcg
     3541 gcgaaaacct ccgtaatctt ttgttgcaac cgccactaaa aggtaaagtt gtcctgggat
     3601 ttgatccggc ttatcgtact ggatgtaaac tagcagtcgt agatgcgaca gggaaagttt
     3661 tagcaatcga agttatttat cctcataaac ctgcagcgca agcaaaaaga gaagctgctg
     3721 gaccagcttt tatccaatta attaataaat atcaagtaga tatggtagct atcggaaatg
     3781 gtacagccag tcgggaatct gaactttttg tggctgagca gttgaaatcc gccgaccata
     3841 aaacctatta cgctatcgtc aacgaagcag gcgcttctgt ttactccgct agcgaaattg
     3901 cacgaaaaga atttccgcat ctacaagttg aagaaagaag tgctgtttct attgcccgtc
     3961 gcctgcaaga tcctttagct gaattagtca aaatcgatcc taaagctgtc ggagtaggcc
     4021 agtaccaaca tgacgtttct caaaaacgtt tggctgaaca attggatttt gttgtggaaa
     4081 cagcggttaa ccaagtaggt gtggatgtga atacagcaag cccgcagctc ttgcagcata
     4141 tatcaggatt gaataaaaca actgcccaaa acattgtcat ttatcgtgaa gaaaacggag
     4201 aattcaccgc acgaactcaa ttgaaaaaag tgccacgctt aggaccaaaa gcatatgaac
     4261 aagcaatcgg attcttgcga gtccctggtg ggaaaaacat tttggacaac acaggtatcc
     4321 atcccgaaag ttattctatt gcaaaagaaa tattaacttc agtccaactt tcagaaaaag
     4381 agcttggtac agaagaagca gtagaaaaat tgacacgatt atccgtagag aaactggctg
     4441 aaagcctatc agtcggagaa gaaacgttag ccgatatttt agctgggctg acacaacctg
     4501 gacgagatat gcgtgatgag atgccagcgc ccttattacg tacagatgta ttgagtatgg
     4561 aagacttgaa accagggatg gaattgactg gaacggtccg taatgtcatt gacttcggcg
     4621 catttgttga tatcggtgtg aagcaagacg gacttgtcca tatatcgaaa ctcagcaaaa
     4681 aattcgtcaa gcatcctaca gatgtcgtgt cagtaggaga tatcgtaaca gtttggatag
     4741 aacaagtcga tacgaaaaaa ggtagaatca gtttgacgat gctttcccct tatgaagagt
     4801 aacaatgagg agtaacaaaa attgacacaa gaagaactgc agcagttagt agaagagata
     4861 tcattaaaaa gcttccagct tcctttcaag caccaagcaa tgttcaatcc acgcttacgt
     4921 acaactggcg ggcgctatca tttaaacgat catcatttag attttaatgc gaagctattt
     4981 gaagagtcag acgaagagac gattgcagga atcatcaaac atgaactttg ccattatcat
     5041 cttcatctag ctggaagagg gtatcagcat aaagatatgg atttcaagta tcttttgaaa
     5101 aaaacaggag ggttgcgcta tgcaccagcc ttgacgaaga aacagataaa gatggagtat
     5161 tatcgatgcc aagattgttc tgccacgatc tgccgcaaga gaagaatcga tgtacgcaaa
     5221 tatcgttgtg gaagatgtca tggagaaatc aaatggattg agacaaaaag agaagacttt
     5281 aatgaagcac aaaaaatcag atgaaaggat agaaggcctt tggaaaaaag tgagcagagg
     5341 aatctaaaag actggaatca gctattaaat tttttagccg cacctgaaga gggaaatttt
     5401 gaggaactga cggcacctac acttatttta gctggaaact gtcttcctat cttagctgat
     5461 aaagcagcac aaatgtattt aaatggccaa gtagatcaat tgtttttggt cggaggggtg
     5521 gggcatgcga ctagaatcct ttatgagaat tttgaaaagc aaggatttca ttttgaagat
     5581 ggcatgagtg aaagtgaaat ctgtcgtcag tatctaaaag aagtctatga tttgcccgat
     5641 aaggcttttc tgatagaatc gaagtctaca aattctggag agaacgccac tttttcactg
     5701 gagattcttc attccttagg agctgtacca gaaaaaatct tattgatgaa tgatccgaca
     5761 ctgcaacgca ggactcgagc aacttttgag aaagtatggc aaaatgaaca aactattttc
     5821 gcgaatgctg tgccatttgt gccggaaatc cttcactttt ctgaagagat catctttaca
     5881 gcaaaagaac tgaatcatca atggcctaag gaatatttcc atgcgctagt attaggagaa
     5941 atggaacgtc tccatgacga tgagaacggc tatggtccga aaggaaaaga ttttattcct
     6001 cacattgata tatcagagga agtttggtct tcttacacca gaattaaaga gtcaataaaa
     6061 acggattttt ctcgtacttg accattttag caagcgaatc ctcctttcca aataaatttg
     6121 gttgaaattt tcaatcgttt ataagataat gaggagcgtg ttgaatgtaa tagctaaaag
     6181 aatggacgct agataagaga agaggattca aatggaaaat caaaataaat tgtatcattt
     6241 tgttggtatc aaaggttcag gaatgagctc cttggctctt gttttacatg aacaaggtct
     6301 aaatgtccaa ggatcagaca ttgaaaaata tttctttaca caaagagatt tagaaaaagc
     6361 aaatatcacg attctgccat ttaacgcaga taatgtaaaa ccagggatga cgatcattgc
     6421 aggaaatgca tttcctgatt cacatgaaga aatccagcga gcaaaagaat taggtttgga
     6481 agtgattcgc tatcacgact ttattggtca ttttatccaa aattatacga gtatcgctgt
     6541 aacaggctct cacggaaaaa caagtacgac tggactgctc tctcatgtat tgagtggtgt
     6601 ccgtccaaca agttatctaa ttggagatgg aacaggacat ggtgatccgc aagcggaatt
     6661 cttttcattt gaggcctgtg aatatcgccg tcatttcctt gcctattcac cagactatgc
     6721 aatcatgacc aatatcgatt ttgatcatcc ggattattat acaagtatcg acgatgtatt
     6781 tacagctttc caaacaatgg ctggacaagt gaaaaaagct atttttgcgt atggtgacga
     6841 tgcttattta cgcaaattaa aagcaaatgt gccgatttat tattatggtg tcacagaaaa
     6901 cgatgatatc caagcacgaa acatcgaacg cacaacttct ggttctgctt ttgatgtcta
     6961 tcatggggat gaatttgttg ggcacttcac tgtaccagca ttcggtaaac acaatatctt
     7021 aaatgcttta ggtgttattg cagtagctta tttcgaaaaa ctggatctga aagaagttgc
     7081 agaagaaatg ctgactttcc caggagtaaa acgtcgattc agtgaaaaga tcgtagcaga
     7141 catgaccgta gtcgatgatt atgcgcatca ccctgcagag atcaaagcaa cgattgatgg
     7201 cgcacgccaa aaatatccag ataaagaaat aattgcagtt ttccaacccc atacgtttac
     7261 gcgtacgatt gcattgatgg atgaatttgc agaagcactt gatttagctg ataaagttta
     7321 cttatgcgat atttttggtt cggctcggga agaacaagga aacgttaaga ttgaagacct
     7381 tggagcgaaa atcaaaaaag gtggagaagt catcaaagaa aataatgtat ctccattgtt
     7441 agattatcat gatgcagtag taatcttcat gggtgctgga gatgttcaaa aatttgaaca
     7501 agcgtatgaa aaacttttaa gcagcacgac gaaaaatgta ctgtaataca attactgcag
     7561 tgtccttata aaaatagaaa gagggggagt ggcaaaaatc ttgccaagcc cccttttttg
     7621 caacttatga taaacggtgt tcaaaagctt gcatctgcgg taataagccc aatcaaatca
     7681 aaaacatgag aagctgtttt ttcttggctg ttcttattat ttgatgcagg acagcttttt
     7741 cacgacctct gtaaactata ggaatctgtt aaatcaaggg agcagaactt gccttttacg
     7801 ctacttttgg tacaataatg tatgattaaa aataaactgg agggtttcta tcttgaacat
     7861 aggtaagccg agaaaactag gaaaaacaat aaatgaaatc gaggaaggcg actcgttatc
     7921 cttaacagaa tctattgaag ataaccagct tttgctgtat ttgggattga cgaatgatgc
     7981 aaatcctttg tatatccaac atgactttgc acaaaaaaca gaatatcaac agccaatcgt
     8041 tccatcagtg atgctgatgg ggatcatcac aagtgcaatt tcaaagcatc ttcctggacc
     8101 aggatcacat gttgtcaatt tttctgtcaa ttttattgaa ccggttttcc attatgaaac
     8161 actgactttt cagtttgagg tcataaaagt agataagatg aaggatgtgg tgacgatttc
     8221 tgtagaagcg gagaatacga acgaagatcg tgtgttggat gctgtcgtta tggtccaacc
     8281 gccacaagtt cctgaattgg aaatagcaat cgaaagtgaa ggtgaattga atgaataatc
     8341 caagtacatc aatgtcaggt atcaatcgtt tttatgcaaa agtttatgga tttttaggta
     8401 tgggacttgc ccttagtgca gtcacttctt acttggctct ttgggtattt cctttgcaat
     8461 tagcaacttt tatcaataat tttccactag gctttgtggg tctgtggatc gttgaactga
     8521 ttttagtaat ggttctaggc gtaaaagcac aaaagaatcc gagcttagca atcggtggat
     8581 ttattgccta ctctcttttg aatggcataa cattagcggt tactcttgca tactacgata
     8641 ttggaacagt aacgcaagct ttcttgacag ctactttgat gttcatgggg atgtctgttg
     8701 tcggagtaac aacaaaaaga gatcttagcg gggttggaag agcaggacag gcagctttaa
     8761 tcggagtgat cattgcacta gtcttgaaca tcttcatttt gagaagtagc gtagttgagc
     8821 tgttcatctc cctactgtta gtcgtcatct ttgcagggat tacagcttat gataaccaaa
     8881 gcatccgtaa tatctatgtc cagacaaacg gaacagcaca tagtggaatt gctgtattca
     8941 tggcattgaa gctgtatttg gactttatca acttgttctt ggcgtttctg agaatctttg
     9001 gacggaacaa ctaaaaaaga aaaaaattca tcagttgggg ctataccgaa ttatttggta
     9061 tggtcccgct ttttcttttc gcattccgca ggattctttg ttagaatgaa taagaacttt
     9121 acagtaagga gttaagaaga tgacaaaaaa taaattatta ctcgttgatg ggaacagtgt
     9181 ggcttttcgc gcattctttg cgcttcacaa ttctcttgaa cgtttcaaaa atagaaacgg
     9241 tcttcataca aatgcaatct atgcattcaa tacgatgttt gaaaatgtta tgcaaaaaga
     9301 gcaaccgact catgttctgg ttgcgttcga tgcaggaaaa acgacttttc ggacagaaat
     9361 gtatgcggag tacaaaggtg gaagatccaa aacacctggc gaattcaaag aacaaatgcc
     9421 atatatacgt gatttgttga caggactagg ggtacagtat tacgaattgc ccaattatga
     9481 agcagatgat attatcggta ctttggcaga aaaagtagac aaagatcaat ttgatgtcgt
     9541 cgtgctttca ggtgatcggg atttgactca attagctagt aaagaagtaa aagtggatat
     9601 cactgtaaaa ggtgtcagcg atatcgaaag ctatacacca gaacatgtag cggaaaaata
     9661 tgatgggttg acgccaaaac agattattga catgaaggga ctagccggag atgcctctga
     9721 caatatcccc ggtgtgacta aaatcggtga aaaaacagct atcaagctat tgaaacaata
     9781 tggctcagtc gaaggaattt atgaacatat cgatgaaatg aagcaaagca aaatgaaaga
     9841 aaatctgata aatgacaaag agcaggcttt cttatcgaaa aaattggcga ccatcaatac
     9901 ttcagcgcct gtggatgtct ctatcgactc attgaagtat gaagggaaaa atctcgataa
     9961 actcgttccg ttttataaag aaatggattt caatcagttc ctttctaaat tgaatatcgt
    10021 agaagaagaa gtaaaaatgg atgatatttt gtttgaagtc gttcatgaat tcaaagagga
    10081 aatgtttact acggatatgg cgttgtacgt ggaaatgatg ggcgacaatt atcatacaga
    10141 ggaaatcgtc ggagtagcgt gggggacaga aaagaaaatt tatgtcacga acgatctatc
    10201 tgtattcgaa aaccgagcgt tccattcttg gatcactgat ccgactcggc tgaaaaaagt
    10261 atatgatgcg aaacgaactt atgttgcttt aaaccgctat actggaaaaa cgaaaggaat
    10321 cgattttgat gtcttgctag gtgcatacct tctcgatacg aatgaaaaaa gcacagatat
    10381 cgaaggaata gctgctcatt acggatacaa cgatatccaa tcagatgaag ctgtatacgg
    10441 caaaggggcg aaaaaaggtt taccagaaga agaggaactc ttttttgctc atttagcgcg
    10501 aaaagtagct gcaatcaatg cgttgacacc aaaattgagc caagaactgg cagaaaaaaa
    10561 ccaagaagat cttttccgga aaatggaact gccattgtct atcattttgg cagagatgga
    10621 aataagcggg atcaaagtag atgcgactcg cttacaagaa atgaagggcg agttctctgc
    10681 tcgcttaaga gagatcgaac aaaaaatcta cgaagaagcc ggcgaagaat tcaatttgaa
    10741 ttcaccaaag caattaggag tgatcttatt tgaaaaaatg ggattacccg ttatcaaaaa
    10801 aacaaaaact ggatattcca cagccgtaga tgtactggaa cagcttcgtg aacaagcacc
    10861 aatcgtagag gatatcctca cctatcgtca aattgcaaaa attcaatcta cctatgtcga
    10921 gggactgctg aaagtgatcc aatcagatgg aaaagtgcat acccgttatg tccagacatt
    10981 gacccaaaca ggacgattaa gctctgttga tcctaatttg cagaacatcc cgattcgatt
    11041 agatgaaggg agaaagatac gtcaggcatt tgttcctaga gaaaaagact ggctgattta
    11101 ttcttctgac tattcacaaa tcgagttacg cgtacttgcg catatctcaa atgatgaaca
    11161 tttgaaagct gccttcctag aaggtcaaga tatccatgcc agcacagcga tgcgtgtatt
    11221 tggaatcgaa aaggcagagg atgtcacacc aaatatgcgt aggcaggcaa aagcagtgaa
    11281 ttttggtatc gtgtatggca tctctgatta cggactttca caaaatctag gaatcagccg
    11341 aaaagcagca cagcaataca tcgatacgta ctttgaaaaa tacccaggtg tcaaagaata
    11401 tatggaaaga atcgtacgcg aagcaaaaga ccaaggctac gtcgaaacgc tgtatcaccg
    11461 ccgtcgatat ctgccagaca tcaattcaag aaattacaat atccgttctt ttgcggaacg
    11521 aacagctatc aatacaccaa tccaaggaag tgctgctgat attttgaaaa tcgcgatgat
    11581 cgaattggat aaacgattaa aagaaacagg tcttcaagct acgatgctgt tacaagttca
    11641 tgatgaactt gtcttcgagg taccggaaaa agaacttgaa tcattagata aattggtgaa
    11701 ggaagttatg gaacaagctg tttctcttca tgtaccactg atcactgaca gcagttgggg
    11761 aaaaacgtgg tacgaagcaa aataaacatt gtttaagaca gagcaatata atgaaggtga
    11821 aaagaaaaaa tgccagaatt accagaagta gaaaccgttc gtaaaggatt ggaacggtta
    11881 gtagtaggaa aaacgattca aaaagttcag gtcttgtggc ccaagatcat cgagcaacca
    11941 gagacaccca tctttgaggc ttctcttgtt ggcgaaacca tccaatccat tggacgtaga
    12001 gggaagtttt taatttttca tctcgatcat tgtgaactga tctcacactt gcgaatggaa
    12061 ggaaaatatc aatttacgaa agaaaataca ccaatcgata aacatacgca tgtacttttc
    12121 ttttttgagg atggaagcca acttcgctac aacgatgtac gaaaatttgg tcgtatgaca
    12181 atcgtcgaaa aaggagcaag tgcaacttac cgaggaatca tgaaattggg acctgagcct
    12241 ttgccagata gcttcttatt agcagatttc gcaaatggac tgaaaaagtc ccacaaagca
    12301 atcaagccat tgctgctaga ccaacgtcta gtgactggtc taggtaatat ttatgtagat
    12361 gaagcattat gggaagcgaa gatccatcca gaacaaccgg cgaacacact gcgccccaaa
    12421 gaggtagaac aattacgtct atcgatcatt gatgttttgg atcgtgcaat agaagccgga
    12481 ggtaccacga ttcgcagtta tttgaatgca ttaggagaat caggcggatt tcaagtagca
    12541 ctgcatgtct atcagcaaac tggcaaacca tgtatacgtt gtggaacgcc gatcgtgaaa
    12601 acaaaagtgg cacaaagggg gacgcactat tgtccgaatt gtcaaaggct aaaaatcgca
    12661 aagtaggatt tgttttaggt ttgacgggtg gaatagctac cggtaaaagt acggcagcca
    12721 aagtctttca gtctcatgga tttcctttga tcgatggcga tgtgattgcc agagaaactg
    12781 ttgagcctgg aacgcttgct ctgaggaaaa ttgtatcagt gtttggccaa gaggtattgc
    12841 agcctgatgg gcaactagat cgagggaaac ttgggatgat tgtttttcca tcaaaggaaa
    12901 aacggcaaaa acttgatcag ctattagatc cgtttatccg aaaagcgata aaagatcaaa
    12961 tcgcctctct aagttccaat catcctttag taattgtaga tatcccattg ctctacgaag
    13021 gtcattatga ccactatatg gatgcagtag ctgtcgttta tactacgcca gaaactcagc
    13081 ttcagcgttt gatgaaacgt aatcaactga cgctagaaca agcgcagcag agaattagta
    13141 gtcaacttcc aatcgaagaa aaaaagcagc gtgcggatat tttgttcgac aacaatggaa
    13201 caaaagaaaa acttgtggga caaatcgaaa attggctttt tgctaatcgc tttctttgag
    13261 tatctgtgtc aattttgcta tcctgagtat atagaaagtg agggatagcg atgggatcat
    13321 taaaaaatca aaaggataaa gtcgtaggta acgcaaaaga aaaaatcggc gagatgctca
    13381 ataaagaaaa tctaatcgat gaaggtcgta cgcaatcaga aacagcagaa gaaaaagaaa
    13441 agcagtacca agcaagaatc gatgaaatag aagaccagca ggcagaagaa gaagcgaagc
    13501 ggcaagattt gactcaagaa cgaaaaaatc cgacacctga tcgtagtaaa acaccgctcc
    13561 cagaaagtga ccacattccc gatgagcaaa aaatcgagga acagcgaatg aaacactata
    13621 caggaatcga agagaagtta taaaatagta aaaaatcgag atgtgacttg aagcagatga
    13681 gaaggtgttc atctgcttca agttttttgt gctagatgac taaatcagct tttgtgtaag
    13741 ttatttctca gtaatcaagg tgatcagtat tggaaaattg aaatttagta aggacagggg
    13801 acacaattac gcatgaaagt agaaaaaatt gttcttatat ccgtgttttt tgggtaggac
    13861 agtcgcgttt gtgatttgtt tcgtgttata atacaactag ttgtaaagaa agacacatcc
    13921 tttatataaa atgcgatgca agaggtgaga caaaatgaga tgtcccagat gtcaacataa
    13981 taattcacga gtaatcgaca gccgtcaagc agatgatgga cgtgcgatcc gtcgccgtcg
    14041 cgaatgtgag aattgttcgt tccgattcac tacatttgaa cgaattgaag ctgctccgtt
    14101 attagtaatc aagaaaaacg gggaacgcga agagttcaat cgcgataaaa ttttaagagg
    14161 actcatccgt tcagcagaaa aaagacctgt agctatggaa caaatggaac aaatcgtcaa
    14221 ccatgtcgaa aaccgcgtac gcagtctcgg agaaaacgaa gtatcgacga atctaattgg
    14281 tgaatatgtg atggaagatt tagttgattt agacgaaatc gcttatattc gttttgcaag
    14341 tgtttatcgc cagttcaaag atatgtctgt tttcttaaaa gagttgcaag atatcgtgga
    14401 taaagcaaaa gaaaataata aagagaataa taaagaaaac aattaagaaa caaaaaggag
    14461 gcatttcctt ggaaaatgct tggaaagagc ttcaaccaaa aaatatttac caaggaagaa
    14521 aagcggctcc gctgagagaa gacagcaaga acgccttgct gtatctttat cagccactaa
    14581 tcggcggaga agccttgagt atctatttca ccctgttatc agaaatctct caagaatctg
    14641 gcaaaggtcc tgaagggatg catgcagatc tgcttagtag tctaggctgc ggtatcccgc
    14701 aattttatga tgcaagaaaa aagctagaag gaatcggctt actggatgtt tattttaaag
    14761 aagatagtga tctgggacct cgttttattt atgagctgtt agaaccactc attcctaaag
    14821 catttttcaa agactctgtt ctggcttttc ttcttctgga aaaagttggt gaacgacgat
    14881 tccagaagct tacccaattc tttgaaccta aaaggcatac agcagaggga tatcaaaaaa
    14941 tcacaaaaaa atttttagat gtttatcgct tcaatgaagc agtgtacgct gctaatcaag
    15001 aaaacatcga atcgctgaat aaatcttttg aagaatctga atcagtagca ttacttgaag
    15061 cagattcgac attcgattgg cagtttttga atgattggct ggcaaaaaaa catattgcaa
    15121 gtcttgatga tccaatcatc aaacaagtcg acatgtatca tcagctttac ggtttcgatg
    15181 aattgacact tggcgaactg atcagtcaat cttatgactt tagtgaacaa aaagtatctg
    15241 aaaaagaact gcaaaaagcc attttggcaa tgcagagacg tccggtgaag caaacaaaag
    15301 aaacagttgc ggtttctgaa aacagtaatg aaccctcgtc aaacgaacat ttgacaaacg
    15361 cagcgcttca gctgataaaa gaagcacaga gcattccacc gatgaaatat ctggaagcaa
    15421 tcaagtcaga aaaaggcgga tatgtttcta aacaagaaaa ctggttactg caagatcttg
    15481 tttctcaatc aggtctttcc agtagcgtga tcaatgtttt gctgaattat gtactagtga
    15541 tcaagaataa tgctagctta aatgccagtt atgtaaatac agtggcaaat gaatgggcac
    15601 aaaagaaaat cgccacagca gaagaagcga tccagcacat tcggcaaata agtaatcaag
    15661 caaaacaatc aaaacaaaag aagcaaacaa attatcaaac tggcaagcga aatgttcgac
    15721 gggaaaagct tcctgattgg gtcaaccagc caaaagatga aaaacagatc agcagcgaga
    15781 aaaaagcaga gattgaccgc cgttttaaag aatacctggc aaaaaaggag ggtgatatct
    15841 agtggaagac gtaggcaaag aattgaagag gatcatcagc aaacgcaatt atcaagaaaa
    15901 gtatgctgaa atgatcgctg aagtcttaaa tgatgcggat gttcaagctt ttctaagcgc
    15961 acaccaagaa gaattgacac aagcggatat tgaaaaaagt tacgctaaat tgtatgaatt
    16021 cgtgcaagaa aagcgtaaat atcaaaaaaa cgatccaacg atgatcgctc ccggctatga
    16081 gccgaagctg acgttgaatt ttcattatgt ggatgtcact tatgtaccaa cggaagcatt
    16141 gttagcacgg caaagacaag aagaaatcag aagccgagta aaagcaatgg atatgccaaa
    16201 agatattcaa tcggcttctt ttgaaaactt tgaacgtaca gatggacgag gatatgcgag
    16261 cttagaagcc ctagattttg tagagaaata tactgctgat ccaaaagggt atcataaagg
    16321 actgtatcta gtaggaagtt ttggtattgg aaaaacatat ttattaggag caacagcgag
    16381 agaattagcg attgctggat tcaccacgac gctagtccat tttccgacat ttgccgttga
    16441 gatgaaacag gcaatcggaa aagatcaagt ggcagaaaag ttagatgcag tgaaacgttc
    16501 acctattttg atgatcgatg acattggtgc agatgcgatg agtagttgga tacgagacga
    16561 agtattcggt gttatccttc aatatcggat gcaggaacag ttaccaacct tcttctcatc
    16621 gaattttaca atggatgaat tggaacagca tttgtccgtc actcaacgtg gagatgaaga
    16681 gccgctaaaa gcgaaacgga taatggaacg catacgttat ttgacaaaag aaatcgaaat
    16741 gactggtcgc aaccgacgca atggttgaac agttgacaag tccaactgac atttgaccta
    16801 gtagtaaaag caggtcagcg aatcattata tggacagaaa tgaccggaga caatcaccgc
    16861 aacaagtaat gcagaaactt aaacggagga ttttttatga gggctatttt ttctagtata
    16921 tctgtagttg gtctattttt tcttttagca ggctgcggta gcgcagatag caacacaggt
    16981 tctgttgatt cgattgatgc gggtcaaaca gtaaaaacaa gcagtacgaa aacggagaga
    17041 caaagcatat ttgtcggaac gattgaaagt atcactcaag tagaagcaga agaagaaacg
    17101 cttcagttga aactcgtcga tgtcatggcc aaagaagatc cagcgaatat gggaacgtca
    17161 ttcagcaatg acggagtgat cttaaatgct actgctgaaa aaataagcgt ggacataaat
    17221 gaattgaaaa tcggtgacaa agtccaattt acattagttg agatgccgat catgacgatg
    17281 tctatcccac cgcaagttcc tggagattcg attattgatg tttcattgaa caaataacca
    17341 ataacatttt ttcaaaaata agaaaatgtt gttttataga acgaaaaggt atatttttta
    17401 aggagatatg ggaatttttt tattcctagt ctccttattt tatttggaaa atgctataat
    17461 tatctagaag gagtgaagct atgaaccatt tagatttatc tcagcgctta gccgcagtgg
    17521 gtgactttgt ccctgaatcc gctcgtttag cagacattgg atcagatcac gcttacttac
    17581 ccgttgcatt gatattaaga ggaaaaatcg aatatgcagt tgctggtgaa gtcgttcaag
    17641 gtccttatga atcagcacgt aagcaagtca aaaaagatgg attagaagac cgtatcaccg
    17701 tacgccttgc aaatggtcta gatgctatcc agccacaaga tggtattaca gctgtcacga
    17761 ttgcgggtat gggaggttca ctgatccgag atatcctaga agctggcaag ttgaatcaac
    17821 gcttaagcgg acaagaacgt ttgatccttc agccaaacat cggagagcgt acattacgat
    17881 cttggctgca agaaaatcag tataaaatcc aatcagaaaa aatacttgaa gaaaataaaa
    17941 aaatatatga aatcattgta gccgaaaaag aacaaaaacc agttacttac aatgaaatgg
    18001 agctgatgtt cgggccattt ctgcttgaag agaaaagtga gatcttcaaa tcaaaatgga
    18061 caagagagct tcaacaaaga aaagctatcc gtgaacagct caaacaagca gctaatcaag
    18121 aggcacgtca aaaacaaatc gagcaggaaa tccaaatgat tgaggaggtt ttaaagccat
    18181 gacaatcagc ggacgcactt tcatccaaaa gtttgaaagc tattgtcctc agtggctagc
    18241 tgaagaagga gacccagtcg gactgcatat cggaacattg gataagccaa tccaacgagt
    18301 aatgatgaca ttagacgttc gtccagaagt ggtcgaagaa gcaatcaaga aaaaaatcga
    18361 tttgctgatt gctaaacatc ctccaatctt tcgtcctgta aaacggctgg ttactgatca
    18421 gccacaagaa aaaatgtatg cagatttgtt gaaacatgat attgccgtct atgcagcaca
    18481 cacgaacatg gatattattt gggatggact gaatgactgg ttttgtgaac ttctcggtat
    18541 tgaagtggag agttatttag taaagactca tgagattcat tataaaaaac tggccgttta
    18601 tgtacctgtg gatcatgcac agaaaatgcg tgaagtatta gctgcagctg gtgctggtac
    18661 acaaggtgat tataccggta caagttttac ctcgattgga cacggtcgat ttactccgga
    18721 agctggtgca caaccagcga ttgggaaagt agggaagaca gaacaagttc aagaggcaaa
    18781 agttgaggtt attttgccgg aaactatcga aaaacaagtg attcaagcca tgcggtcagc
    18841 tcatccttat gaggaacctg cttatgatct tttcgctata gatgaaccag tcgaaatgtt
    18901 tgggttagga cgagtaggtg aattgccgca agaaatttct atagaggcat ttgtagaaca
    18961 agtaaaagaa gcgttccaac tagatggact gagaatagtc cagccaaaaa atgcaaaatc
    19021 ctctgtgaag cgcattgcga tttgcggtgg aagcggtgaa aaattttatc cgcaagcaat
    19081 cgcccaaaga gcagatgtct atatcacggg agatatctat tatcatacgg ctcatgatat
    19141 gcaaagcgct gggttgattg caattgatcc cggtcattat attgaatcgt tatgcaagca
    19201 acgatttatc gagaaatttg aatcttggaa acaagaagaa aattgggata ttaacttttt
    19261 cgtttctgaa acaaatacaa acccatttca atttaattaa aaggagagaa tactatgtac
    19321 gaaaatcttt tgccacgttt tttacgatat gtcaaaacag aaacacgctc cgatgcaaca
    19381 agcaaaacga ctccatcgac tcagacacaa gttgcttttg cgcaagaatt gaaaaaagaa
    19441 ttagaagagt taggcttagc tgatgttttc tataatgaag ataacggatt cgttatggga
    19501 acattaccaa gcaatgttga tcatgaagtg cgttccattg gatttatttc ccacatggat
    19561 acagcagatt ttaatgcagt aggagtcaat ccacaaatca ttgaaaacta tgatggcgag
    19621 tctacgatcg tactagatgc ggaagaacgc tatacattga atacaaaaga ttttccaaat
    19681 ttgaaaaatt atgaaggcca cacattgatc acaacagatg gcagtacatt actgggagca
    19741 gatgacaaat cagggattgc ggaaatcatg actgcaatgg agatcttgat caataatcct
    19801 tctatccctc acggtaccat caaagtggct tttggtcctg atgaagaaat cggtgtcgga
    19861 gcggataaat tcgatgttga gcaattcgat gtagattttg cttatacagt cgatggtggt
    19921 cctttaggtg agttgcagta tgaaacgttt agtgctgctc aagcaaatat tacgatccaa
    19981 gggaaaaatg tacatccagg gacggcaaaa gatacaatga tcaatgctct acagttagca
    20041 atcgattttc ataatcagct tccagccgat gaagttccag aaaaaacgga aggatacgaa
    20101 ggtttcttcc atttgatggc attggatggg aatcctgaag aagcttctat gagttatatc
    20161 attcgcgatc atgatcgtga aaaattcgaa gcacgcaaag cacaaatcac acatatccaa
    20221 gaagagttga atcagcgatt tgatcaagaa cgaatcaaag tagatatgta tgatcaatat
    20281 tacaacatga aagaaatcat tgaaaaagat atgagcatcg tggaattagc gaaacaagca
    20341 atgatcgagc ttggtatctc tccagtgatc gaaccagttc gtggaggaac agatggttca
    20401 aaaatctctt acatgggtat tccgacgcca aatctatttg ccggaggaga aaatatgcac
    20461 ggacgattcg aatttgtttc tttacaagta atggaaaaag caacagacgt gattgtaaaa
    20521 attgcagaat tgaacacaaa gagttaaaat cgaaaaagaa cagcttatga caaggcgttc
    20581 gtcccaatga tggaatgtca tctgtctaag gctaaagggc aggacaatgg aattggttgt
    20641 cccgccctct tttttgaaga gtaaatagga ggtaaagaat ggaaaagctg ctagtttttt
    20701 ataacaaaag ttcagggaaa gatgagggtg agaaattagc acgctggttt caagattatg
    20761 taaaagaaaa gcagccagaa ttatatgtag aactagctga aactggccca tcgattgatg
    20821 atgcggaatt agtcaagaaa gcagaagcca tagaagctga tactttagta attataggtg
    20881 gagacggcac gattcatcat atcgtccaag cgttccaaaa aacattatct caataccatg
    20941 tcggattact tccaggtgga actgtcaaca accttgcacg tgttcttgag attcccctca
    21001 aaaaagaaga agctgctgac gtgatcctgg aaggcaatgt acggaaaata gactatggtc
    21061 aagtcaatga tgaagtcatt atctctacac tgacaatcgg aatcttagca gatacagcag
    21121 tttggatcag tcaagaagaa aaacagcgtt acggctcgtg gatctttatc aagaagtttt
    21181 ttaagttatt gctgaagaag aaaaaatatc atttggattt agaaacagac gaggagcatt
    21241 ggaaaggaaa atcccaactg ttgacagtca cgatgtctaa ctccgtgggc ggttttacaa
    21301 attttgacga ctcagcgacc ctagatgatg gaaaatttca tgtaacgatc gtaccttcct
    21361 tagatatttt ccgatttatt ttttatttgc ctagaatcat gagaggcaaa ttctatacga
    21421 tccccggcat tacttatttc acagccaaac gcatgaaaat ccaagcgcag gaaaaatctg
    21481 tgggcactcg aacggatgga gacacgacag atgatcttcc agttgaatta tctgttgtat
    21541 caaaaggatt aacagtttat tcaccaaaga aagaagaaaa agcttagaaa tagtagttaa
    21601 cagaaagttg gaagaagagt aatggatgta ttgttacaca atcctttgtg gttttacctt
    21661 ttcattgtga atttttactt attttgtctg atggggtatg ataaacatca ggctagaact
    21721 agcggctggc gtgttcctga gaataatttg atatttgtca gcctgttagg cggtggtttg
    21781 ggaggattga tctctcgaaa agttttccat cataaaactc ggaaaaagaa gtttactata
    21841 ggattcgctg caggtgtact tgttgatttg attctcattt attttttcca ttaaggagca
    21901 ggagatgtcg tctaaaggaa cgaaaactaa aatcattctg aatctgcttt tgctgattgt
    21961 gatttttgcc attatttatt atttaatcaa tcaatcattt gcagatatct ttgccgaatt
    22021 gctgtctacc agtgttgttg ttttggcagc ggctgttata ttagggacag tttaccaaat
    22081 cgctgaaggg cgctcaatca aagaaattgc tgaacctttc gcgccgaatt ttacaacgaa
    22141 agatgggttc ttgacctcta gctatattgc gttttaccga atcgtttcgt ttggtacagg
    22201 aacgctgatc tcggagattt atttttataa caaaaaagga atgaagctat cccagggagc
    22261 aggggtgaca gcactccata tgattatgta caaactggca gtgatcactt atgcattagc
    22321 tggcttggtc atccaattct ctctttttta ttctaatggt ccatcaatga tctggtttat
    22381 catcgctggt atccttttga cgatcgtgat tgttgccttt ttattggcga tgtccgtcag
    22441 cttgaatgtt caaatatttt ttgtcagctt atcgaataag ttatttaaaa gtgagcgttt
    22501 gcggaaatgg gtggatacat gcaatcagca gatctactct ttaagggaaa cggtccaaac
    22561 cattatccaa gacaggactg cgctattgcg gatttatcta tggaatatgt taaaacttgt
    22621 attttggtat gtacttcctt atttgattct gattgaaaac catcctaaaa tagattttct
    22681 actgaccatt tcgtttatca gctttgcagt agtgctttca ggggttattc ctacgcctgc
    22741 tggtattggt tcctttgaat ttgtctattt gttattattc agaccagttg ttggaacagt
    22801 tgacgcagtt tcttcgcttt tactgtatcg tttcagttcc tttatcttgc catttctcta
    22861 tggatttgtg tatgtgattg ctgaacggcg aagagtgatc aaagaagaaa taatagaagt
    22921 aaaaaaagag aataaagaaa atcaagggta ggtcttttat aagatctacc ctttttatca
    22981 aatcaaagaa aaacgttatg gataagataa ttttacgttt gtttggctct ttttttatat
    23041 acatacaatc aattttgttt ttttaagatt tatataagtc gagaaaaaaa gtcaaaaaac
    23101 tatgtgtata ttttgaccaa aattgaccaa agcgatataa tagtttttgt aaagatcaaa
    23161 gaaataagtg atcttacctt taattaggag ggtatacctg tatgaatatt gagaaaatga
    23221 ctacgacgct tcaagaagcg attgctgaag ctcaacagat tgcggtcact cgaaaacatc
    23281 aagatatcga cattgcccat gtatggaaaa tctttttaca atctaatcat tttggtcgaa
    23341 atttctatac ggatgccgga ttggatgtcg aatcttttga acatgaaatt gatcgtctac
    23401 tggatgagta tccagttgtt tctggtggaa acgtccaata tggacaaaac ctcagccaga
    23461 atttctttcg attattgaat gaagcagacc aaatacgtga aagcttcggc gacgagtttc
    23521 tttcaacgga agtcgttatt ttagcgttga tgaaattaaa aaattatccg ttaactgttt
    23581 atttgaataa aaacggatta tctgaaaaag aattgcgtaa aaacatcgaa gagatgagag
    23641 gaggagaaag agtgacctca caaaaccaag aagaacaata caaagcatta gaaaaatacg
    23701 gcgttgattt agtacagcaa gtcaaaagcg gaaaaatgga tccaatcatc ggtcgtgatg
    23761 aagaaatccg tgatgtcatc cgtattcttt cacgaaaaac aaaaaataat cctgttctga
    23821 taggagaacc cggtgtaggg aaaacggcaa tcgttgaagg gcttgctcaa cgtatcgtac
    23881 gcaaagatgt gcctgaaaat ttaaaagata aaacgatttt ttcattagat atgggtgcat
    23941 tgatcgctgg agcaaaattc cgtggtgaat ttgaagaaag actaaaagca gtcttgaaag
    24001 aagtcaaaaa aagtgatgga cgtatcctct tgttcatcga tgaaatccac aatatcgtag
    24061 gggcaggaaa aacagaagga agtatggatg ccggaaatct gttgaaacca atgcttgctc
    24121 gcggtgaatt acatttgatc ggtgccacaa ctttagatga gtaccgtcaa tatatggaaa
    24181 aagacaaagc gttggaacgt cgctttcaaa aggtattagt caaagaacca acagtggaag
    24241 atacgatcag tattctgcgt ggattaaaag aacgttttga gatccaccat ggtgttaaca
    24301 tccatgacaa cgcattagta gcagcagcta ctttatctga ccgttatatt actgatcggt
    24361 tcttaccgga caaagcaatc gatttgatcg atgaagccag tgcaacgatt cgggtagaaa
    24421 tgaactctat gccaacggaa ttagatcaag ttactagacg tttgatgcag ttggaaatcg
    24481 aagaagctgc actgaaaaaa gaatcagatg atgcaagtaa aaaacgcttg aaaaatttgc
    24541 aagaagaatt agctgaactt cgtgaagaag caaatgcgat gaagatgcag tgggaaacag
    24601 aaaaagaaga agtcaattct gtttcagcaa aacgtgctga aatcgataaa gcaaaacacg
    24661 aattagaaga tgcagaaaat aactatgatt tagaacgcgc tgctgtattg cgtcatggta
    24721 cgatacctca attagaaaaa gaattgaagg aattagaagc taaagcaaaa gacagcgaga
    24781 ttaagatggt tcaagagtcc gttaccgaaa atgagattgc tcaagtcgtt ggcagattaa
    24841 cgggtatccc cgtaacgaaa cttgtagaag gtgaacgaga aaaactaatt aaattgaatg
    24901 aaacacttca taaacgagtg attggtcaag atgaagcagt tgatgcggtc agtgacgcgg
    24961 taattcgttc acgggctgga ttgcaagacc caaatcgtcc tctaggttct ttccttttcc
    25021 taggaccaac tggtgtcgga aaaaccgaat tagcgaaagc gttagctgag aacttgtttg
    25081 actcagaaga tcatatggta cggatcgata tgagtgaata tatggaaaaa catgctgtgt
    25141 ctcgtctagt cggagctcct ccaggctatg taggttacga agaaggtggt caattaacgg
    25201 aagctgtcag acgaaatcct tatacaatcg tcttgttgga tgaaatcgaa aaagcgcatc
    25261 ccgatgtctt caatatcttg ttacaagtcc tagatgacgg acgcttgact gattcaaaag
    25321 ggcgagtagt agatttcaaa aatacggttc tgatcatgac cagcaatatc ggttcacagt
    25381 tactgctgga aggcgtgact gctgatggaa cgattccaga agcagtggct gaacaagtca
    25441 acacgctatt acgtggaaac ttcaaaccag aattcttgaa ccgaatcgac gacacgattt
    25501 tatttacacc attgagccta gacaacgtga aaggaatcgt tgacaaaatg gtggcgcaat
    25561 tggcacaacg tctggaacat caagaaatcc tgctgacaat cagtgatgaa gccaaaacgt
    25621 ggattgctga aaatgcttat gaaccagcat atggtgcaag accattgaaa cgatttatca
    25681 ctaaggaagt agaaacacca ctagctaaag aaatcgttgc aggacatgtt atgcctaaat
    25741 caaaagtaac gattacttta cttgatggac aacttcattt caaaacagaa gagctagaag
    25801 aaatcgtcta atcttccatt gagactataa aagaaaaaag gggtctgaca caactagtgt
    25861 cagacctcct tttttcaaat aaacagtgtt ccagaagtag tttcttcgaa aaataagccg
    25921 aaatttcttg aagctgcccg cttctgtcac aacctctttt attaggtatt tttcactttt
    25981 tctaatttat aactttcaaa gtaacgctca tcttttttac tttgagacca tgcaaacaat
    26041 cccattaagg cagcacctat cgtatttgtc agcaagtcgc ccatcgtatc catcaatgct
    26101 gctcgtccga caaacagtgt gccatcagaa gcagcaaaac gttgaagatt catgcctaag
    26161 aactggtcgc ataagaattc ccagaactcc caaaacacgc cgcaaagtcc agcaaacgca
    26221 aagccgaata agaggaacaa ccaaggactg gtttttgata tctcagcatc tttcatcaag
    26281 taaccaatca atccatagcc aagagctgtc aggaccattg gactcaccgc atgtaagatt
    26341 ttgtcccaaa aagagatgat actgatcaaa tgcatccctg tgcctaaaaa gacagagatg
    26401 aacaagaaaa accagtaaaa caaaacgatt gctgctggta ttttaagttt gaaaactttc
    26461 aaaatgactt gtggcatgaa aatcaatgca atccctaata aagattccac gataaatgtg
    26521 attccttgag aagtcgaata tttttgtgtg ataaattcat aaaaattata gcagacaacg
    26581 aatacaccaa aagcaagtaa agctatttgc acctttttga tttctttaat cactgaaagt
    26641 ccctccatct aaaaatcttt ttacttctga aagcaactca atttctgaag aaaccacttt
    26701 tccgttcaat ttgatcaaac ctattgtata taagttcagg tagtgaaatt gattttcagc
    26761 tgtttcttgt agtgcagcga tttttttcgg gtgggttccg ccttgctgtc gagtatcagt
    26821 atataagccg atcactggga tgcctttcgc ataagccaca ccgatttccg atgctacccc
    26881 ggcatcgatc gtcagcccat ctagtaaagc aatcattaaa tcactagcca acactttttc
    26941 tgtgtcagca agagcaatca ttcgactgtc tgcataagca gttttgtcat tgattgcttc
    27001 attttcttga ggaagataga tcgaaagctc agaagagagc tctctgattt ctttaactaa
    27061 ctgttggtta tataacaaat cacttttagc aaacattggt gcggcaaaat aaattttcat
    27121 ctaaacatca tcctttttat caaaaataat cgctaaagta agcttccatg tttggaatga
    27181 tacgctctag tgacttcagt gtttcttcat ctgtttctaa ccgttcgatt cttgccaaat
    27241 aagaagcacg gcgataattc atcatcaagc aagtcaatgt ttggatatcc agtcgaacgg
    27301 attttcctat aggttcattt aagacacgga cttgtccctg ttcatcccat gttaaagcga
    27361 agacaccatt attccaagga gcgacaggat cttcaatgat gaaatgaaaa gcatcagctg
    27421 ttccaacaaa cggaaaccgt tgaaggaatt cttttacatc aacgatccga gccataaaat
    27481 atggttcgat ctgctctttg atctgactat cttcaatcaa aaatgctaaa ggctcatttt
    27541 tataaatatc tccatgtacc cagtagatca tggaaaaatg tgcggaaata aagttccata
    27601 agccatttcg agcttcttga tcaagataga acatttcttt tacgtgaaat atttcttcag
    27661 ctacccagta gaacagaaca cctcttggtt catggttgct atcgtagtag acagcggctg
    27721 tgcgctcatc ttcattttcg aaacgccaat actcttccca atgaaaatca ctgcggaata
    27781 aagcaccatg gttttgttgg gcaaatttgt cataaacgga aaatacgtct ggatgatcaa
    27841 ctggtaatcg ctcaaccata ccacttactg ggatttgttt aggtagctgt gtatctctga
    27901 ttttaaagga aagtttatca gacatgattt cccatccttt acgccgatag taggggatac
    27961 tgtaaggata caaataagag atccattgct tattcttgcg catattatcc aatgcaacga
    28021 taattagatc ttgcatcaat ccatggttag catattctgg ataagttcca actccagtca
    28081 caccacccat tttataacgt gcaccgtgaa tattgacttc acaaggatag atagcgattt
    28141 gagagatcag tttattttcg tggaaccatc cgaacacttt cgatacttcc aaaatcggtt
    28201 ttttggaacg gataaaagct cgtttattct caaacccgct ttcctcgatg tctgcttcag
    28261 tcacttggaa cacataggaa agcaattcat tgaattgatc gatatgttct tgagtgaccg
    28321 gttttaattc aagattttct ctaaaatctt tttgatccat tttttcatct ccatttatta
    28381 gcttgataac agtatagcaa aattctacaa aaaaaacttc tatagctgac taattaaaaa
    28441 ctctttaaat tactgctttt cgttggtata atggaaagtg ctgattatat gaaaagagga
    28501 gacgctatgg atataaagaa aatgaaagaa cgccaagagc agattcgtaa tttttcgatc
    28561 atcgctcata tcgaccatgg gaaatctaca ttggccgatc gtattttaga aatgacgcac
    28621 actgtgacgt ctcgcgaaat gcaagatcag ctgttagact cgatggattt ggaacgtgaa
    28681 cgtggaatca cgattaaatt aaatgctgta gaactcaatt acacagcgaa aaatggagaa
    28741 acatatatct ttcacttgat tgacacccca gggcacgtcg acttcaccta tgaagtctct
    28801 cgaagccttg ctgcctgtga gggtgccgtt ttagtggtcg atgcagcaca aggaatcgaa
    28861 gcacaaacat tggccaatgt ttatttagcg ttagacaatg atttggagat cctgccagtc
    28921 atcaataaaa tcgacctacc cgcagcagat cccgaacgtg tccgtcaaga aatcgaagac
    28981 gtaatcggaa tcgatgctag tgaagctgta ttagccagtg ccaaatcagg gattgggatt
    29041 gaagatattc tcgagcagat cgtggaatat gttcctgcac caagtggaga cttagatgca
    29101 ccgctaaaag ctttgatctt tgactctatt tatgatagtt accgcggtgt tgttctaaat
    29161 gtccggatca cggacggtat ggtcaaaccc ggagacaaaa tcaaactgat gagtaatggc
    29221 aaaacatttg atgttacaga ggtcggggtc ttctcgccaa aagcagttgc tagagacttt
    29281 ttgatggtcg gtgatgtcgg ttatatcaca gcaagtatca aaactgtcca agatacacga
    29341 gtaggggata cagtaacgtt agcagacaat cctgccgctg aagctttacc aggttaccga
    29401 aaaatgaatc cgatggttta ttgtggtctt tatccaattg atacttctcg ttacaacgat
    29461 ttgcgggaag ctttagaaaa actgcaattg aatgatgctg ctttacaatt cgaaccagaa
    29521 acctctcaag cattaggttt tgggttccgc tgtggattcc tcggacttct tcatatggat
    29581 gtcgtccaag aacgcttgga acgggaattc aatctagaat tgatcacgac tgcaccatcc
    29641 gttatttacc atgttaataa aacggatggt acgactgcta cagtagataa cccggcagat
    29701 tttcctgaac ctgtaacgat tcaggatgta gaagaaccat tcgtcaaagc acagatcatg
    29761 gtcccaaatg attttgtcgg tgcagtaatg gaactttctc aacgaaaacg gggagaattc
    29821 attacgatgg attatttaga tgattaccga gtaaatgtcg tctataatat tccgctttct
    29881 gaaattgtct ttgacttttt cgacaaattg aaatcaagca cgaaaggcta tgcctcattg
    29941 gattatgaaa tgtctggtta tcaaaaaagc aagttagtga agatggacat cttgctgaat
    30001 ggagaaaaag tcgatgcatt aagctttatc gttcaccgag actttgccta tgaacgtgga
    30061 aaagcaatcg ttgaaaaact gaaaaaacta atcccacgcc aacaatttga ggtgccaatc
    30121 caagcagcta ttggacaaaa aatcgttgcc cgttcagaca tcaaagcctt gcgtaaaaac
    30181 gtcttggcta aatgttatgg tggagatgtt tctcgtaaac gtaaactctt agagaaacaa
    30241 aaagaaggga agaagcggat gaaacaaatc ggctccgtcg aagttccgca agaagccttc
    30301 atggccgttc tgaaaatgga cgaggacgaa ccgaagaaat agcaagggtt tgtttgaaaa
    30361 cagtgtaaaa aataaaccat gtttacatgg atttgccacc ggtttgccac cggtggtttt
    30421 tttcggtggc aaaaatcaag aattctttct aagattttcc atgatatcga ccgtttcatt
    30481 tctcatctta tctgttacat gcgagtaagt atccatcgta attgaaattc tgctgtggcc
    30541 caatcgttca gagatttctt tcatttttgc gccattttcg agaagaagtg tagcatgagt
    30601 atgtctgaga gaatggaagt taaaagagag ggagagtgca ttcgatatcc tcctagtatt
    30661 ccatttcact acacttggcg taactagctc accgtcttcc ttcgtacata ctgcatttga
    30721 atcaatgtag agctttccat acttcattcg attttctaat tgttgtttct tatgtttttt
    30781 cagaattgct agcaaggttt gtccaataaa aatcgttcga ttggaactgc ttgtctttgg
    30841 cgtaccatat acccatgcgc cgtcattctt taccatttgt ttctctacag taattgttcc
    30901 atttgaaaaa tcgacatcat cccacgtcag accacaaact tcgccaacgc gcattcccgt
    30961 ataaaatcca atattcaaag gaatatagaa aggatgtcct tcaggagtaa tttctagcat
    31021 atgatcaaag tcctcaagag aaatgatttt tagatctttt ttagtcgttg gtcgtgcttc
    31081 atattttggt atttttacat acagcatagg attttgcttg attaatcccc aaggataaac
    31141 cgccatattc agcgcattct taaggacaga gtgagtaata gtcattgttt tcttcgagta
    31201 acccttttta aattcagcat tgatgaaatt ttgtaaaaga gcaggggaga gatccgtaag
    31261 ttttttcttt cctaaataac cgtttatatg atttttgatg gtaaatcggt agttttcata
    31321 ggtattgtat tttagattta gtttaacgta ttcctccatc caaaaatcaa ggtattgttt
    31381 tactcgagta tccgtaccta aaaagtattg tcctgtttcg tcaatatctg ataaaacttt
    31441 tcgtaaagca gcttcggcct ctggtcgggt gtctccgcca actttctcca ctttttttct
    31501 tgagccatca tcattgatat c
//
LOCUS       DACDYJ010000027        31464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDYJ010000027 DACDYJ010000000
VERSION     DACDYJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.027"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            340..660
                     /locus_tag="IWM51_001290"
     CDS             340..660
                     /locus_tag="IWM51_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP9970719.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            814..1719
                     /locus_tag="IWM51_001291"
     CDS             814..1719
                     /locus_tag="IWM51_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9970720.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1716..2489
                     /locus_tag="IWM51_001292"
     CDS             1716..2489
                     /locus_tag="IWM51_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9970721.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2670..4094)
                     /locus_tag="IWM51_001293"
     CDS             complement(2670..4094)
                     /locus_tag="IWM51_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9970722.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4305..4751
                     /gene="nrdI"
                     /locus_tag="IWM51_001294"
     CDS             4305..4751
                     /gene="nrdI"
                     /locus_tag="IWM51_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP9970723.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4816..6798)
                     /locus_tag="IWM51_001295"
     CDS             complement(4816..6798)
                     /locus_tag="IWM51_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9970724.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSAATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6812..7708)
                     /gene="murQ"
                     /locus_tag="IWM51_001296"
     CDS             complement(6812..7708)
                     /gene="murQ"
                     /locus_tag="IWM51_001296"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9970725.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7711..8772)
                     /locus_tag="IWM51_001297"
     CDS             complement(7711..8772)
                     /locus_tag="IWM51_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9970726.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8983..9510)
                     /locus_tag="IWM51_001298"
     CDS             complement(8983..9510)
                     /locus_tag="IWM51_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9970727.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9577..11079)
                     /gene="lsa"
                     /locus_tag="IWM51_001299"
     CDS             complement(9577..11079)
                     /gene="lsa"
                     /locus_tag="IWM51_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9970728.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKSQPKHLSISYVRQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11337..12458)
                     /locus_tag="IWM51_001300"
     CDS             complement(11337..12458)
                     /locus_tag="IWM51_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9970729.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPHGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12583..13524
                     /locus_tag="IWM51_001301"
     CDS             12583..13524
                     /locus_tag="IWM51_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9970730.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFRSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKTAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYNPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13674..14570)
                     /locus_tag="IWM51_001302"
     CDS             complement(13674..14570)
                     /locus_tag="IWM51_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP9970731.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFNMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLAYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPIPSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14839..16179
                     /locus_tag="IWM51_001303"
     CDS             14839..16179
                     /locus_tag="IWM51_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9970732.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVIVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16397..16840
                     /locus_tag="IWM51_001304"
     CDS             16397..16840
                     /locus_tag="IWM51_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP9970733.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17105..18523)
                     /gene="nhaC"
                     /locus_tag="IWM51_001305"
     CDS             complement(17105..18523)
                     /gene="nhaC"
                     /locus_tag="IWM51_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9970734.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(18926..20149)
                     /locus_tag="IWM51_001306"
     CDS             complement(18926..20149)
                     /locus_tag="IWM51_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP9970735.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVEQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20180..21019)
                     /locus_tag="IWM51_001307"
     CDS             complement(20180..21019)
                     /locus_tag="IWM51_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP9970736.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETTVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDEKNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21036..21716)
                     /locus_tag="IWM51_001308"
     CDS             complement(21036..21716)
                     /locus_tag="IWM51_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9970737.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21718..22791)
                     /locus_tag="IWM51_001309"
     CDS             complement(21718..22791)
                     /locus_tag="IWM51_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9970738.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IIEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKINILQKNELDSRIKVIEGGR"
     gene            complement(23437..24108)
                     /locus_tag="IWM51_001310"
     CDS             complement(23437..24108)
                     /locus_tag="IWM51_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9970739.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYFDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(24195..26873)
                     /gene="mgtA"
                     /locus_tag="IWM51_001311"
     CDS             complement(24195..26873)
                     /gene="mgtA"
                     /locus_tag="IWM51_001311"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP9970740.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLTFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASMFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVDGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNVTFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIHRFQELL"
     gene            27532..27969
                     /gene="ndk"
                     /locus_tag="IWM51_001312"
     CDS             27532..27969
                     /gene="ndk"
                     /locus_tag="IWM51_001312"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP9970741.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAVDAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(27991..28857)
                     /locus_tag="IWM51_001313"
     CDS             complement(27991..28857)
                     /locus_tag="IWM51_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9970742.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICSKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLSLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEKKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29017..31110)
                     /locus_tag="IWM51_001314"
     CDS             complement(29017..31110)
                     /locus_tag="IWM51_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9970743.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWMKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVLYWLDIATFIGILVWLTIKKIPIKSQPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFSESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSAIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWNEFDN
                     AQMQRVPLMIHIPGYTKGSVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSPQHD
                     QVVAFRNGNFVTPKYTILGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKSALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNIAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKDNQ"
BASE COUNT         9121 a         6562 c         5561 g        10220 t
ORIGIN      
        1 cttattgtaa ggtaaagctt tttttaattt attgcccgat aatatataga aataaggtct
       61 agttactgtt ttttttaaat gattaataaa taacaacttc tttctatgaa attgcaaagc
      121 attcatcttc ttttccgcta acgatatatc cgtcagtgct tggtacactt tactaaaaag
      181 tatgagattg ccgatatctg gataggtgtg ctatactaca gaaaaagata tacgatctgc
      241 caccgggtag gataaagagg atgatcattc cattaggtct tttaagggat gattttcctc
      301 tttttgtgta tttttttata gttaaggagg aatgtaagta tggcatggat ggaattaatc
      361 atagcaggaa ttttggaagt attctggtca acgatgatga aatggagtga tggtttttct
      421 aaaatcaatt attcttttta cacagtgatt ggcatgattg ctagttttta ctttttgtca
      481 aaggcaatca aaagtttgcc gatgagtctc tcttatccta tctggacagg gattggcgca
      541 gttggttcgg tcataattgg tgtggtcttg tttcatgata agctgaatct ttccacttgg
      601 ttctttgtgg ggatgctact catcagtatc attggcatca agattactag cggccactag
      661 acacttggaa taagtactga tcagaaaaaa taagcctttt tatttaatat tcaatttttt
      721 tatgaaaaaa taagaaataa gtccaatgac ctagcgactt ctccactcat caggtgtatg
      781 cttgtactat ctatagaaaa ggagtgaata tttttgaaaa gtctttcggt ccaagaatta
      841 tccaagaact ttggtcacaa acaagccctc agcgatgtca gttttagctt atcccctggc
      901 aaaatcgttg gattagtagg tcctaatggc gctggaaaaa caacgatcat gaaagctatt
      961 ttaggactga tccattactc agatggaaca atctgtattg gtgaaaatga gatttctccc
     1021 tctagtcata aagggttgga acaagtagga gcattgatcg aatacccggg tatctatcca
     1081 ttcttaacag gctatgatca tctgaagtta ttctctgaaa ccaacgatgt atctgctatc
     1141 gacactatcg tgaatcagct caagatggaa aagtacattc acaaaaaagc gaaatcctac
     1201 tcgttgggaa tgaaacaaaa attgggaatt gctcttgcct tactgaacca tccgtctatc
     1261 gttattttag atgagccgat gaacggtttg gatccacaag caacaagaga tgtgcgaaaa
     1321 atcattaccg acttggctac tcagggcacc tctttcttga tctccagtca cttactcagc
     1381 gagcttgaaa aaatggtcga tgagctgatc ctgatcaatc aaggaaagat catcaaacaa
     1441 tgtacaatgg ctgaattgaa ccaaacagcg caagatttca tcgttatctc tacaacaaat
     1501 gatctagctg ctcgaacgat cttgacagaa gctggatata ctttggaaga tcctgataaa
     1561 atcaagcttt taaaagaaaa cgaagaatta ttaggaaccg tgatccagct attgtctgca
     1621 caaaatatcc atgtgacaga cgtccagcat ctgacgaatg atttggaatc ttctctattg
     1681 gaaattctgg attcagtaaa gggggaagaa gaaaaatgac attgcttgta aaacaagaat
     1741 tatttaaatt gatcaagaaa aagtcaactg ctgttttatc tgttctatta gttgttttgc
     1801 tcataggaac agcacttcta gccaaaaaat acacgacgat cattgatcca gttgaaatga
     1861 ctgctcagct ttttagtgcc acatcttgga ttgtctttat catgattgca gctgcaagca
     1921 cgatcatttc gatggaagca caatatggta cattgaaaaa tctcctttat cgaaagtatt
     1981 ctcgaggaga aatattagta agcaaatgga tcacattagt tatttattct gtttatttat
     2041 accttttagc tattattgtc actgtgttga tgaaactgat tcttttccct tcgatttcct
     2101 tcacggagaa ggtttctact ggtcaaacac tgatccaaag cttgttcact tatactttgg
     2161 gtagttatat cggtttatgg ctgattttaa gtttagtgtt gatgattgct tgctttatca
     2221 atagttccgg tgcatccatc tcagcaggaa tcgtctttta ttttgcttcg tcaatcatat
     2281 ctggtatcct tattgcactc atccaaaaat gggagtggat caaatggaat ccgatcagta
     2341 tgttgaatct gcaaaaccaa attggaaacg aagagatcat gaagcaattg actcatcttt
     2401 ctactaatca aatgctattc ggtaatttag cttacatcgt tttgttctta gcattaggat
     2461 atctcgtatt taagcgaaaa aatgtatgat gactcatcaa aaaagaggct gtgaaagaga
     2521 cgttcagcct tattgccgat tggctgtctc ttaaacaccg tttattctga aaaaggagct
     2581 gtgacaacat ttgtgtcaca gctcctttcc tagtatgttt ttgaataaac gacaggctca
     2641 caacctctct ttatacttct tttatacttc tatacgtgaa gcatcccagt ggcaaataaa
     2701 accattgcaa tcactccgac catcgtcaca cctataagta aaatcttatc taagtggata
     2761 tgttgtacag gctcgttttg ctccttacgt gctttgaggt aaaaataaat gcctggcaag
     2821 aaaagaacaa gaacaagcat tacttcatta aaggctacga ctagcatagc ccatacttga
     2881 aaggcacttg ctaaaagccc tactaaaaat tggacccatt ctttccgctg ataagaaagt
     2941 ttcatttggt acaatcctaa gaaaagccac gtgaagaaaa tagctgctgt accaagtgtg
     3001 taagcaaagt tataagcact atcagtgaat aataatgaaa gtaaaaagac attcgtacat
     3061 aacgtcgtca atacgatagc ataagttggt gccccatttt tgttgagttt tccccatgct
     3121 ttaggcaaaa gtccgtcttc agccatgttt cccattgttt caatcggtaa gatcgtccat
     3181 gagagccaaa caccaactaa agaaatgatc aaaccgccgt tgatcacgac tgctccccat
     3241 ttaccaacca tcatttcaaa cacatagccc atagcaggct gcgccttgat cgtttgcagc
     3301 tgattcatcg tcattgtccc atatggaagc atggaaacca agacgtaaat aataatcaag
     3361 ccgaatatcc ctagaatcgt tgctttccca acatctgatt gtttttttgc tcgtgaacct
     3421 acgacagagg ctccttcaat accaacaaat acccaaagca tcaccatgat gcaactcaat
     3481 acttgactgg aaaacgggat cggctgtcca ttcgcattca atataatgtc gctagaaaag
     3541 ttatcaatga attgttggat cgaaaacgtg ctgatagcta ctacaataaa taaaaataat
     3601 ggcactaatt tacagaatgt gacgatagca ttgacgaaac tagcattttc gactccccga
     3661 ttcactagcc atgcatatcc ccataaaaag actgtactca tcacgatcga tgccagattt
     3721 tgcccacctt caaatactgg gaagaaatat cctaatgcgc tcatcaacaa tgttgcaaaa
     3781 gctacgttgc ctaaccagca tgtgagccag taaccccaac cgctgataaa accagcgaaa
     3841 cggccaaaac cttcttctgc gtaactaaag atcccgcctt ttaaatctgg acgtttttta
     3901 ttgatattat tgatcgacaa ggagagcatc aacattgctg ttcctacgag tacccaactg
     3961 aaaatagctg ggccgccagc cgatccggag atgtccgtca taataccaaa tacgccacct
     4021 ccaatcgctg aaccaattac taaggcagca agtggaatta aaccaattcc ttttttctgt
     4081 tgctctgtat ccatgtaagc atcctccaaa ataaaaccgc ttttatcaaa aagtataaca
     4141 aacctttttt atttttactt aattaataaa aactttaata gaaatataat tctaccggct
     4201 tttttctcat tatacaagca aagcgcctag tccctttcca aattacacca ccaaaaaact
     4261 taaaaaagat gctatactaa aaggaaagaa ttggaggatg atccatgaac attttatata
     4321 tatcgatttc aggaaatacg cgtgcctttg ccaaacatct agcagaatat gctgaaaaaa
     4381 tgcatgcaga agatcctgtc aatcctgaag ttactttaaa agaaatccat gaaaatagtg
     4441 attttacgaa agaaacagaa ccatttttta ctttcgttcc tacttattta gacggcggaa
     4501 atggtttaga taacggtgat acagaaatct tgaccgaaac gatgcgtgaa tatctggaac
     4561 atgaagacaa tcataaactt tgtcttggtg tagtcggtag cggaaataaa aacttcaata
     4621 atcagtattg tctaacagcc aaacaatatg cacaaacatt tggctttcct ttcttggcag
     4681 attatgaatt acggggaaca ccatctgatg tgacacgaat ttatcagatt ttagcagaaa
     4741 acaataaata ataaaaaaga agaagctgaa aaatagattt cagtttcttc tttcgtttct
     4801 attgtttcta tcatattaag ctttgacatc catcacaggt gcttttgctt ttgctttacc
     4861 tgttttttcc aagacaaagt tttttacacg atccatattg gttaccaaga cgatcatgga
     4921 tgtttctttg cctgctgctt tgatcgcttc taagtcagct tctgctacaa gcgtatcagg
     4981 tgtcacttgc tgcccttctg ttactttcac atcaaatggc gtaccgccta agtcaactgt
     5041 gttgattccc atatgaagca agatttctaa accatttggc gttgtgatcc caatagcgtg
     5101 tttcgttggg aagacagagc tgactacccc agtaactgga gaatagatat gtccgttttc
     5161 tggttctaca aagtagcctt cacccatcat cttttgtgca aaaactggat cacttgcttt
     5221 atctaaagaa acaactgttc cgtcagcaac tgatgagagg atagtcacac tttcagctga
     5281 agtttgaggt actacttttt caggcacttc atttactgtg gctgtttcag tcgctaattc
     5341 ttctttttct aaacggtctt taggaatacc gaagaagaat gtagcaacga agccaccagc
     5401 ataagctgcc aataatccaa ggatgtagcc taaccattta ccgtcagaga tcaatgggat
     5461 caaagcggct cctgaaggtc caatagcgat agccccgaca tttccgatca ttccgatcac
     5521 tgccccgccg atacctccac cgatacaagc agtaataaat gggcgtccta gaggtaatgt
     5581 tactccgtaa atcaatggtt cgccgattcc taggatcccg actggtaaag ctcctttgat
     5641 catttctgtt aattctttat cttttttgca gcgaatccat aatgccaatg ctgcacccac
     5701 ttgtcctgca ccagccatcg caagaattgg aagcaatagc gtacgaccag tttcatcaat
     5761 catttgcaca tggattggag tcaaaatttg atgcagtccg aacataacca tcggcaagaa
     5821 taatgtgcct agaacgaagc cggcaaatgc tccaccaacg ccaagtaccc agttgatccc
     5881 accgaccatg ccatcagaaa tgaaacctgc taaaggcatg atgaagaaaa tttcaatcag
     5941 accgatggca atcaatgaaa gagttggtgt aacgatgata tctaccgcat caggtactac
     6001 tttatgcagt tttttctcaa cgattgacaa tagccaaaca gcgaagatga cacctaggat
     6061 cccaccttgt ccagctgcaa gagcttcacc agtaaataag tttttcagtg gtgcttccgg
     6121 attcatccct gtcagcatca caacggcccc gataactcca ccaagagttg gtgttgcacc
     6181 aaaaacttgt gcagcattga tacctgtata gataactagg tagctaaaca tcccgttttt
     6241 caagatgttc atcacatcga tatattgttg ccatgttgct gcatcaagtg ttccggctgt
     6301 gactaagttt gctaaaactg cggcaatccc tgccacgatc ccagtaccga caaatgcagg
     6361 aatcattggc acaaagatat tagaaatgtc ttttaatacc gatttgattt tagatggttt
     6421 ttgtttcgat ttttgtgccg ctttcatttc ttgtgcccgt tcgtttacac ggtcttttcc
     6481 agatgctgat gaatttgctg tagctgcact tacagaagaa agtttttctc ctaatttcac
     6541 gcctgccaaa tcaaccattt gctgtgctac tttgttcact ttcccaggtc cgatgatgac
     6601 ttgaagctgt tcatcttcta ctacaccaag cactcctgga atcgctttta attcagagac
     6661 atttacaaga tcatcattga tgatcttcat acgcacacga gtcatgcagt ggacaatgct
     6721 ttctacattg cccatgccgc ctgccccttt ataaatttgt tttgccagtt cttccaaagt
     6781 cagttcgttt tttgccatgg tcttttcccc ctcataaggt cccttttaca aatccgtttg
     6841 cttcagctaa ttttgcttga gcttcctctt tatcactatt tgtcaaaatc atgacgatag
     6901 ccagcttaac attttgatct gcttcttcaa agtacttttc agctgtttcg taatcagctt
     6961 ctgtcgcttg catgatgatt cgttttgagc gttcgattag tttttcattt gttggtttga
     7021 catcgaccat caagttattg aatactttac ctataccaat catactgata gtagaaatca
     7081 tattcaagat aagtttttgt gctgttcctg acttcaatcg agtagagcct gtcaaaaatt
     7141 ctggacctgc atcgacttcg attgggaaat ccgcgtgttt actgatttcg gcttgtttat
     7201 tgcaagaaat cgttcctgtg acagcaccga tcgattttgc gtaatctaat ccaccgatca
     7261 catatggtgt acgaccgcta gctgcgatac caataactat atcatttttt gtcaaattca
     7321 gcttgattag atcttcttgc cctagctcat aagaatcttc ggccccttcg acagctacag
     7381 tcattgcttc ttctccacca gcaattagac ctatgaccat ttccggttct acaccgaatg
     7441 tcggtacgca ttcagcagca tctaacacac ctagtcttcc acttgttccg gctcccatat
     7501 aaatcagacg tccacctttt ttaaaggact caatcgtgtt agcaatcact ggttcgatac
     7561 ttgtcagttc tttttcaacc gcagcggcca ctttctgatc ttctttgttc attagttgaa
     7621 cggcttgaga aatactcatc tcatctaatc caaaagtttc ttggtttctg cgttccgttg
     7681 ttaggttctc taaattgatt ttgctcattt ttatcttcct tcttttctta taaattgata
     7741 gttcatacca gctttgattt ggttgatcaa tggtagctct tctttgataa ctttagctac
     7801 tacattcact ttttcatccg ctggcaaatc atatttggtt aattggatct cacccatata
     7861 gcgcaaatac ttttcgttat ctaaagtaat agaccctttc atacgcgtag ctgtatttct
     7921 tgctgggata ggcggaatct ctcgaaaacg tgcatctgcg cttcgtatga catctcttgc
     7981 atcgtcttgc cgattcgtat gcttccctaa tatatattcg taaaattctt catctacagg
     8041 ttccgtatgg agcaagactg tctgttcttt ttggaaagaa gcaaattgct cctgaacatt
     8101 ttctgacaga cctgcatctc cgatgtaaac aatatccgtg ttacttgcta atagatctag
     8161 agcgcctgca agaggatgga tccctcgatg ttcttccaaa gttggcaatc cttgatatag
     8221 tgggcctctc aactctgtat ctcctggtac aaaaccttgg acaaggagac cttgaatttt
     8281 gagccattga ttttttttct gataccattc tttgtccagc cctgtttcgg gacgtggata
     8341 ataattatgc caagcttcaa tctgtgagaa atcagcttct gcttctttca attcatcgat
     8401 atctttaggc gtgatcgtgc tagcattcaa actgattttc aattgatgtg accagtcggc
     8461 aatctgctga ttggaaatat gataatccat tcttaaacct gttacaccaa ttgctttcaa
     8521 aggcgttaga tcttctaatg aaaaacccgc tcgttccaat gcctctcctg aaatatcgac
     8581 catcaattct agtttattct ttttcgcttc gcttcctagt tcgatcaatc gcttttgata
     8641 tgccgaaaca tcatcttctg gtatatgcat ggaagtgaaa ataccgacaa agccattgga
     8701 tgccatttgg cggatatact gtttcttctc ttctgacaaa gaatcgttca taaatattga
     8761 aaagccaaac atattatccc tctttttctg tatccgtctt catttctatg actagagtat
     8821 atatctaaag aaacaaaatt tcaatacttt tattaaaata attaaatttc atttctaaag
     8881 gttgagttta tattatcaaa agaattgtca taacctaaaa aaggcactgc taagcaaaag
     8941 cagtgccttg aaatatttta tttaaataac acaaaaacat aattactcat aagaaaccaa
     9001 ttctgtatta tctaccatga atgtaaagaa cccctcatta ggcatcggat gaaaataagc
     9061 attgatcact cttatcaagg cattatgaca aactaacaga tagacatttt cttcgtcttc
     9121 gatgcattct ttcaataaag gaacgatccg tgcatacaca tctaagactg actccccatt
     9181 tgggaatcgt acagcgaacg ctaaacgtgc tttttgaaag ttctcatctt tgcttggcag
     9241 tccatcataa tccccaaaat ccatttctac caatcgttca tcaacggtca gcggcaagct
     9301 gagacgatca gcaactgctt gtgctgtatc tcttgctcgt tgaagagggg aatggatgat
     9361 tttcgtgatt ggaacaggca aatctactac cttttctgct agtttctgtg cctgcatctg
     9421 tcctttttca gtcagcggca catctgcccg tccgcaaact aatccttgtg cgttccaagt
     9481 tgtttctccg tgtctcgtta cataaagcat gtgattctct ccttcgttgg caaagttccc
     9541 cttaaccata ccttgttgtt cttgagatta caagggttag attctctcaa gagaaataat
     9601 ctcagtagat actttatcca ggaaggtttg atcatgttcc actagtaaca tggcaggttt
     9661 cacgttcaag atcagttgtt ctaattgttc ttgattgaag acatccaaat aattcaatgg
     9721 ttcatcccat gtatatagtt cagctggctg tgataaagat ttagccaatt ccactttttt
     9781 ccgttggccc atactcatct gctcgatctt gttatgaaaa acatctcttt ccatcccaag
     9841 ctttcggagg ttgttcaaaa atgcttggta gtctacttgg tttttctctg caaattccgc
     9901 caacgttcct cgattgtctt cataattttg gcgtacataa ctaatgctca gatgttttgg
     9961 ctgggatttt tctcctataa ccttgccgtt gaatgccccc agaagataat ggataatgga
    10021 tgactttcct gcaccgttag gacctgaaat cgccacccgc tgatgaggct cgattgtaaa
    10081 atgaattggc tcgaataaca gattttcata ccctaattga agatcttcta cgcttaaaag
    10141 tcgcttatgg tgagacgctt ggctattcat cgtcaaagaa tcgatatatt cgatatcttt
    10201 taatagtttt tctttctcgc tgatctgggt ctccatccga ttcacgattg ctttggatcg
    10261 tttcatcgtc cgtgcagcat cagcacccac tgctccttta ttcactcgtc tagattcagt
    10321 atcgatgaat ccgacttgtt tctttgtttt atctccttct cgggaacgag accattcggc
    10381 tttttcagct gctgttttct ttagcctact gacttctttt ttcaattttt cattttgagc
    10441 catttcaaat tcatcacgaa gttttttctg ttcttcatag atagagaaat tcccttgata
    10501 aagttccagt tgactttttt cgattgctaa aacatggtcc actacttcat cgataaatcc
    10561 ccggtcatgg ctgaccacga tgaagccttg ttttttcttt ttcaaataag ccgctacttg
    10621 ttttctacca gagatatcca aatgattcgt tggttcatcg attaacggga aatgagtgtc
    10681 atccacaaat aaaagtgcca gtaggacttt tgtcttctct ccccccgata gtgtgctgaa
    10741 ttctctccat aagatttcag gatctgtttg catcaattgg agctctcttt cgatttccca
    10801 tatctcaaaa tccgtaatat catttaacac gtaataggtt aaacgttctt tatcttttgt
    10861 ctgttgcggg aaataggcaa attcttgctg atggattact tgcccttgat aaggtagttt
    10921 gttttgtaga atattcagta aagttgtctt tcctcgaccg tttcgtccga taagtcctag
    10981 tttccattgt gtgtcaaaat ttagatttgc ttgttcaaat aataatgtgc cttggctgtc
    11041 gtagccgaat gtcagatttt ttatttcgat tttagacatt ttccatcacc tcgtaaaaaa
    11101 agaccagtcc tattttcaga actggctaaa gaggatgcaa gggttggata cacacaaaaa
    11161 gacccataca tcgaaaaaac caagccaatc ccgaaaatct gcacactgct tccttgtctc
    11221 cacaaggtac agtctgaatg cagctttcat caatcgactt aatttttcaa tgcacggatc
    11281 cctcactttg tcctatattt gaaattacga tagcataaag aaaatgattt tgtcaactac
    11341 ttcgggagga tggtgaaaaa tctgctaagt gtctgtccta agatttttgt tttttctaat
    11401 gtttgcctgt gttccattgc ttcttctaaa cctattggac ccaaatgaat acttaagaca
    11461 attgggagaa aatcagagag tccttctagc tcttggtctc tacttccgca aatagcgatt
    11521 attggttttc gatatttttt tgcaagcact gctacaccgt gtggaacttt tccgtggatc
    11581 gtttgctgat cgatccgccc ttctcctgta atgatgacat ctgcttgttc gatctttttt
    11641 tccaatccag tgatttggct gactaaatcg aaaccagaaa ccatcttcgc tcccaacaaa
    11701 aaaagggctc caccaatacc gcctgctgct ccacttcctg gaatatcatc taaatccacc
    11761 tcccagaaat cttttatttg ctgagcaatc tttcctgctt gtttatctaa gaaagctagc
    11821 gtcgtatcta ttcctccttt ttgtcttcca aattgccaag cagctccttg ttgacctgta
    11881 tatggattgg taacatctgc agcgataatc aattttactt tagaaacaag aacgtttctt
    11941 cgatcaaaat gaagtttttt agtagataat aaaggatttc cttctgtgta cccttctaaa
    12001 gaagcaccca aggattggag taatcctaga ccaccatcgc tacacccgct tcctcctaaa
    12061 gtgacaataa tttgttctac ttcttgaagc agtgcatctt tgataagttc tcctaaggca
    12121 taactggaag caagtcggat agtttgatcc gttggtttat caagtagatc taaccccaat
    12181 gcttgtgcag attcgatgac tgcggtattt tttccttgcc aagttgtcaa taaatagctg
    12241 atttttcgct gacggaataa aagatccatc ccatcaatgg tacgccatgt tcccccgagc
    12301 gcgtaataga ttgcttctgc agtattttct ccgccatctg caatcggcac attgattttt
    12361 cttcctttac gatcttccca gccatttaga actgcctgat ttagttcaaa tgaagaagca
    12421 cttcctttaa atgaatcaat tgccgccaaa atatccatct gtatccctca ctttcttctt
    12481 aagtaagaca ctttttttca tttttttcaa acaaaacaat gctattttct tccatttttc
    12541 tatatactaa aagggaactc attgataaag gagagaaaaa acatgaaaat cgcaatagct
    12601 ggagccggag caatgggcag ccgaatcgga ttgatgctac atcaaagcgg aaatgaggtc
    12661 ctcttgatcg atcgttggcc tgcacacatt gaagccattc gtacaaatgg gttgattgct
    12721 gactttaacg gtaaagaagt ggtcgcaaaa ctgccaatct attcaccaga agaaatcatc
    12781 gaaagcaacg aacacgtgga cttgatcgta gcattaacaa aagcgaatca attagatgat
    12841 atgtttcgtt cgatccaatc cattattact gacaatactt atgttttgtg tttactaaat
    12901 ggattaggtc atgaagatgt actggaaaaa tatgtgccaa agaaaaatat cttatttggg
    12961 atcacgatgt ggacagctgg tctagctggt cccggaaaag tcacattgtt aggtgatgga
    13021 gaaatcgaac tagagaactt ggaaccagaa ggcgaagcat ttacaaaaaa agttgtggaa
    13081 gtctttcaag aagcaaaatt aaatccgatc tatagccata atgttcgtta ttccatttgg
    13141 cgaaaagctt gtgtaaatgg aacattgaat ggtttatgca cgattttgga ttgtaatatt
    13201 gctgaactag gcgcgcaaaa aacggcagag agcatggttc gaacaatcgt atctgaattt
    13261 gcttctatcg cagcaaaaga aggaatcata ttagatcaag aagaagtgta ccaacatatt
    13321 gcttctactt ataatcctga caacatcggg ttgcattacc catctatgta ccaagacctg
    13381 atcaaaaacc atcgtttgac tgaaatcgac tacatcaatg gtgccgtttg gcgaaaagga
    13441 caaaaatatg atattgctac accatattgt gcctttttga cccaattagt ccatgcaaaa
    13501 gaaggaatcc ttggcgcaga ataaaaggct ctctgatgag aatcagctga atcagcagct
    13561 ggttctcttt ttttaagccc aatgaaagat caaaaagaaa acacaactct catgatgcca
    13621 taagaattgt gcttgtttgt ttcggtacat aatctcttca aaatgtaggt tttttattta
    13681 ttttcaacag aggagaggtc atattcgtat tgctcataaa tagtggaatc gttagtgata
    13741 aatcgttttg cccaattttc tgcatcataa aattccaata gctctgatgg gattggctta
    13801 aaaggtgtat gcagaaattc cggtaattca tctaaatcac gttctcgaag caaaaaaata
    13861 ttttcaggac gataaaaatg ataatgagca atcgcttgat tatccgtcac tagtttcttt
    13921 tgaaaataca tagattcaaa tgtccgcaaa gtcactcctt gctgaccctc ccgcaacagt
    13981 tccagaatcc cgcgcgattc ccctgttttt ttcaggtact cttcataagc aagatatcca
    14041 ggttgttcat tccctcgatg tggaagaatg aaatagttgg tcgtaacatc tctttttata
    14101 aattcctgct tatagctttc tgctcgttct tttctcccat tattcgttgc accaaaaaat
    14161 agatccgtct ttggttcgct agaagaaaaa tccatatgtt tcgaataaaa tgaactatta
    14221 tgtttcagcc caagttcttt tgcttccaaa aaatcaaaat gataaatctc atcgattgcc
    14281 ggttcatctt gtgcctgctt cagcatcgcc tcgtgttctg ctttgaagtg attccaaaaa
    14341 aataacacaa tttttgcatt ggtaaatggg cgcacgcgtc gagccatctc catattaaat
    14401 ccataatcta aaaaaacgat cagctgataa tttgccaact gttgtctagc tgatttaatg
    14461 aattccgact gtagtaaatc ttttccggac caaacatcta aagagcggat ggaaggcaaa
    14521 aaataaagtg atttattttt tgcaactagc agtacgtcat ttttgttcat cttttccgcc
    14581 tcactttctc tgtcactttt tcctatgata gcctgtttaa atagtaaaaa gccagtaaaa
    14641 cgcttcctat atttgtatga aaatttaaca tttgtatgaa aatatgatga aataaatcat
    14701 tcttctatta aaattcgcct aaaagttttc tgaatagttg taattgtctg aaaatttgtt
    14761 atcattagtc attgttaaat attgctattt attcttaata taaattttga ttgtgaattt
    14821 aaaagaaaga ggtcatttat ggaagataaa aaattgaaaa aacaacttag ctccagacat
    14881 atcaccatgt tagctttagg tggagcaatc ggtgcaggtc tatttaaagg aagtggcgaa
    14941 gcgattggga tcgccggccc ttctgtattg attgcttttt tgattggcgg gctgatttta
    15001 tatatcgtga tgaaaggtct gggaaaaatc gttttaagcg gtggcgacac gcatcacgga
    15061 ttatctggtt tgatacgccc ttatctaggt gctcattctg ctgattttac tgattgggtc
    15121 tactggtcta tgtggattat caatatcatc gctgaagctg ttgcagctgc ttcctttttg
    15181 cagttgtggt tcccgcacat tccagcctgg gtatttgttt ttatgttggc tgttttaact
    15241 acgatcatca atctttattc ggtacggtta ttcgcagaaa cagaatattg gctggctttt
    15301 gctaaaatca gtgtcattat cttactaatc atttttgcta cttatcttgt cggtcagcaa
    15361 atgttgggca caggcgtttt ccctaccctt cagcaattga ctgaccatgg tgggtttact
    15421 cctcatggta tgaagggaat catcagttct ttgcttgtag ttatctattc ttacggcggt
    15481 tctgaactga ttgctatcac ggtaagcgaa gcagatgatc ctaaacgtgc gattcctaaa
    15541 gccatcaaag gtgtcatggg aagaatcatt tccttttaca tcattccttt gtttttgctt
    15601 ttgattcttt ttccttggaa tactttagca ggcacgaatg tcagtccttt tgtgattgta
    15661 tttgaaaaaa tgaatatccc atttgcagcg gatatcgtca attttgttat tgtccttgct
    15721 ttgttctcat ctatcaattc gggtgtgtat gcatcttcaa gaattttgtt cttccgtttg
    15781 aaggatcgaa aaggttcctc tagaaaacta gctgttttaa acaagcatca agtaccgcaa
    15841 cgtgctgttt tattttgtac cagtgttctt tatcttggtg tagctctttc ttattttgtc
    15901 ggtgacaaat tatttggcta tctggcaggt tccctttcgt atacagtatt attgatctgg
    15961 atcattatca gtgcggcatc ctttgtactg gcactaagaa aaggaacatt atgggataaa
    16021 ggaatcagct tttttgcatt agctgtctta ggattgattt tccttggtat cctgttcacg
    16081 aactccattg gggtaactgt attgactggc ctgctttacc tgttcattta tttcagttat
    16141 caaaaaaaga atgatgcgtt cgtattgacg aatgaatgaa atcgatagaa aattttattt
    16201 tatttatgtg gaatagatgg aactattgaa gaaaggagtt gtgacaaaag tcttgttgca
    16261 actccttttt ccgaataaac agtctcatga cctcttggta aacggtgttc aaaaaccctc
    16321 tgtgcaaaat aattgattgt tctccatgct catgacatga tagaagaaag gaatgaaaaa
    16381 aaggagtttg ttttaaatga aaaagtattg gaagtttttg ttgatgatcg gtgtttcaac
    16441 ggtagttatg tttggtatga tgtacttgaa tgtctatgcg ctcgatcata tcttcttcag
    16501 tcgtacacga gtattcatgg cgttgatgat gggtgcagtc atggcagtca tcatgctgtt
    16561 gttcatgtgg aaaatgtatg aaaacaaaaa gataaatgtt atgatcttag ccgctagtct
    16621 tcttgttttt gccggttcct tgtttatggt acgaagccaa actactgtca acgacacagc
    16681 ttggatgaag gccatgattc ctcatcattc tatcgctatc ctgacaagta aacgagctga
    16741 tttaaaagat cctgaagtaa aaaaattagc ggaagaaatc atccaggcac aagaagaaga
    16801 gatcgacaaa atgaaaaaaa tgatcaaaga aatggaatga ccattatccg tccaaataaa
    16861 aaaagaggat gttccagaag tgatcagctt caagaaataa gaagaagcta cttctggaac
    16921 accgtgaata aggggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca
    16981 aaaatagctt ctcctatttt tcgtttggtt aggcttatta ccgcagatgc aagcttttga
    17041 acaccgttta ttcgtaaaag ggattgtgac aaaaaaagtc ttgtcacaat ccctttttat
    17101 gtttttattg ttctaattgc gctgtcttgc ttactgcttc ttgcggtttc acaaattttt
    17161 ttgagattcc ccaacctgtg aaggcactga ccatcgaaaa cacaggagaa agcaaactga
    17221 aaaatacgaa tggcaagtaa gtgagtgtag gaataccaaa tgtacttgct acgaaacttc
    17281 cggcaattcc ccaaggcact aagtagttga ttactgttcc accatcttct agcgttctac
    17341 ctaatacagt tggatcaaat cctgcttttt cataagtttc tttaaatgca ttaccaggca
    17401 gaataacaga taggaattgt tcaccaataa aaatatttac tccgatactt gtcatcaatg
    17461 ttgtaaaaat caattttggt gtgttcgtca tctttcttga tactcgttcc atcacggttg
    17521 agatcaagcc aaattcgatc aacaatccac ctaatgacaa agttagaata atcaatgaca
    17581 cagtaggcat catactttcg attcctccgc gactaagcag tgaatcgatt tgttggtttc
    17641 cagtatttga aacaaaccct gattcgatga ccccagccaa cttcgttaag gaagtttgtg
    17701 ggttttgaat aaaaatcatt cctactgcta cagcgatatt caacaaaatc gtcaaaattg
    17761 ccggcacctt tttccatgca cacagtaaca ttaaaaataa aggaatcagt gaccagattg
    17821 aaatagtaaa gtttgcttct agaacatcag tcacttcacg aatagatgaa agacttgcgg
    17881 ctttacttgt gtgtcctaaa atcatgaata aaaagaaaga aacaacaaat gccggaacag
    17941 ttgaccacat catatgcttg atgtgtttga acaaatcggc atcaacaata gctgctgcta
    18001 gatttgttga ttcggataat ggagacatct tatctccaaa aacggcacct gaaacaatag
    18061 ctccaacaac cagcgctgga tttaggccta gtgttgtccc aataccgaaa aatgcaatcc
    18121 caatcgttga cataacagta aaggcactcc ctacagcgct acctacgatt gcacacacaa
    18181 caaatacaga agggacaaac cactggacac tgatcaattt gaacccaatg accattagag
    18241 ttggaatgat ccctgcttgt atccatacag caatcaatga acctactaaa ataaaaataa
    18301 agattgggat gattcctggt ttgatgccat tgatgattcc ttcgtgcagc caatctgtcg
    18361 ggtatctttt tatcactgca tacaacatga ccattccgat cgctgctaag atcgtgatat
    18421 taggtgagac ctttaatccg acaacgccaa tcgcaatgcc taaaacaata accattaata
    18481 caagtgttgc ttctaaaaaa cttggtgttc ctttttttcc catgataatc tctccttttt
    18541 tagttgttct tatgttctaa aaaagttaag atatcggatt actccgccgc aattcatccg
    18601 ctattcctcc tttataaaaa taaaaagacc tgttttaaac taaggacgaa tgactccgcg
    18661 gtaccacctt aattaaaaac agatctgttt ttcactttca aacttttatt caaatgtatg
    18721 gactttccat gtaattcgtc aattaggctt gatcggttca cagcgaccac cgactttctg
    18781 aacaaaacac ctaaccgcta ctgcgtgaaa agttgacttg tatgaagttt accttccata
    18841 agatcatttg tcaatgaaaa agaaaatatt ttttcatttt tctgaaaatt atttttataa
    18901 ctttcacaca caagtttatt ttttactaga aagtagcacc ctcttgtttt tttcttgcta
    18961 ctatttcttg atgttcttgc ttgattcttt ctaatagctt tggctctttc aacagatcaa
    19021 gagctgtatt ggcgagtagt tctgctgcaa tcgcaataga agcaaggcct ttgtcacttt
    19081 tagcagcagc tttgaattct tctgtgtgtc cggcaatata tgtctcagag attgctaccg
    19141 taggctggat tgttggtatg acttggctga catttcctac atcagaagaa ccgagacttg
    19201 ttctttgttc cgtttctatt tcttcatctg gaacacctgc ttcttttgca tgctggaaga
    19261 ataagtcatc aaaagaagga gtaacgatga catcatcaac tgcattttgg aacaatccaa
    19321 actcaaaagt cgtgcccgta cttatcgcag cacctcgtac gatattttcc actttttcat
    19381 atactttatc taatgtttgc cgatttcctg cacgtaaata gaaacgtcca gcagcgtaat
    19441 ctggaatgac gttggcagct actcctccgt ctgtaatgat cccatggata ttgacatctt
    19501 ttggcaattg caagcgtaga gcgttgattc cattaaacac ttggatcaat gcttccaaag
    19561 catttactcc tttttcaggt gctgctgctg catgggaagc gacaccgtaa aatttgatgt
    19621 cgactggatc attcgctaaa gattctgtcg ttttcccata acgaaaagct ggatggacgc
    19681 ataacgctac atcgacatcc tcaaaaaaac cttctcgaac aaagctgcct ttagctgatc
    19741 cgttctcgcc cccttcttcc cctggggtgc catatacacg gatttctcca ccagtttcat
    19801 caataaattg ctccactatg ctggcggcta atacggagta agtaccaaac aaattatgcc
    19861 cgcatgcatg accaatacct ggtaaagcat cgaattctgc caaaaaggca attgtgggtc
    19921 cgggtttatt ggaaatataa cgtgcatcaa aggctgttcg gtgtccagct acttcttttt
    19981 ctacccaaaa cccttctttt tctaattgtt taatcaacgc tgctgaggaa taaaactcat
    20041 agttacttac ttcaggatgt tcgtggatat ctagagcaag tgcttgatat tctggtaaac
    20101 gatattggat tagatccttc aattggtttg ttgagattgt tgttgtcata aaaaattcct
    20161 cctatgctag tcatttttct tattttctcc cgaatgtttc ccatgcaggc acgcttgatc
    20221 cttttgatgt tttctcgatt actttgactg tgtcttctgt ttgataagcg tctactattt
    20281 tttgatacgt tttatttttt tcatcttctt ttcttgcaac gatgatattc acataaggac
    20341 gtgcatggtc atccactggt tctaagaaga tggcatcaga agcaggatta aatccagcat
    20401 ctacagctac accgctattg atcactgaaa tgtctacatc tgacaaagca cgtgctgttt
    20461 gggaagcatc taattcagaa atgtctagat tttttctatt ctcggtaata tcagaaacag
    20521 tgggcgcttg tccttttgcc ttatccactt tgatcaatcc agcagtttgc aataacagaa
    20581 gagctctgcc gccatttgta acatcattag ggattgctac ttttgctccg tctttgatat
    20641 cagctacatc cttcaaagtg ttagaataaa ttcccagcgg tgcattgaca gtatttccga
    20701 tcgatactaa atcagttccg tgttctttat tgtaattgtc taaaaagaat tgatgttgga
    20761 aagaattcaa atcaatcgat ccatctgcta aagccgcatt tggttgattg taatctgtaa
    20821 atttcacgta ttccaaatta atgttctcct ttttcaaacg gtctttcacg ttgtcccata
    20881 catctgtatc ttctccaata attcctagtt ttacggtagt ttcttttttg tcagttgtat
    20941 ctgcttttcc ttggctacac cctgccaacc aaataattgc tacaaatgta atgactacaa
    21001 aacgaataat ttttttcatt ttttctcctc cactttcaat gtgtaatttt tttaatcagt
    21061 tgattactga taaattgact taaaaaaact aagattagaa tcaataatgt agccacaatc
    21121 gttacatctg tttggaatcg attataccct ctcgatatag caagattccc taatccgcct
    21181 gcacctactg ctccagccat ggtagtcagt ccgatcacgt tgatgatagt caacgccgag
    21241 acccgaatga taccaggcaa accttctaac aaatacacac gtaaaataat accgattgga
    21301 ctggttccca tcgcttctgc cgcttcgatc accccaggat caacttctag cagtgcgttc
    21361 tcgatttgac gagcaaaaaa cggaacaact ccaaaaatca atggcactaa agctgctttc
    21421 gcaccaatcg tcgttccaac taagaaacga gtagtcaatg ctaaaagaga aagcaagata
    21481 atgaaaggga tggaacgaat gatattgatc agcttatcaa gtatttcaaa tagcagacgg
    21541 ttagctagta atccattagg gcgtgtcact actaacgtca cacctaagat gattccgatc
    21601 atcccgccaa tgaatgctgt ccaaaaagac atatatagtg tttgcttcgt cgcttcgaca
    21661 aattcatccg gaatcaaata aacattgggc aaatatgttt taaagaaatc agtcatttta
    21721 tcttcctcct tcgatcactt tgatacgact atctaattcg tttttttgta aaatatttat
    21781 ttttacacca gatttgatca aataatccaa tgctttttgc cgctgtttgg tttctccagc
    21841 taaagtaacg atcaaactcc ctaacggcgt ttcttgtaat aactctacat tgccatataa
    21901 aatattcgct gttacttgat atttgctgta cagatgagcg attagaggtt cgttcgtctg
    21961 atttccaata taacttaatt ccaccagcca tttattggcg atctgatcgg caaaagcaga
    22021 atgggcaatg atcgtttcta atgcttggtc aagatgagta gctgttcgaa taaaatcttt
    22081 ggtcaattct tcttcaggat tgctgaagat ctggatgcta ctaccttttt caatgattct
    22141 tccatcttcc ataaccgcta ccttgttgca gatttctttg acaacttgca tctcatgagt
    22201 gatcaggacg attgttaaac ctaactggcg attcaatttt tttaacaatg caaggatctg
    22261 aagggtcgtt ttcggatcaa gtgcactggt tgcttcatca cataataata ccttaggttc
    22321 attcgccaaa gctcgtgcga ttgctactcg ttgtttttgt ccccccgaca gttgtttagg
    22381 ataagcagat gctttttctt ccaaaccgac aagttccagt agttcattca ctttttgacg
    22441 acgttcctgt ttcgattttc cagaatactt taaagaaaaa tcaacgttgt caaaaatagt
    22501 tcggctttcc attagattga aatgctgaaa gatcataccg atggcctttc tttcttttcg
    22561 caattctttg ggagagagct gactcaaatt cgtctgattg acaatgactt ctcctgctgt
    22621 tggtttctgc agcaaattca tcacacgaac taacgtactt ttccctgcac ctgagtaacc
    22681 gacgattcca taaacatcgc ctttttcaat cgttaaatct acagaatcta ctgcctgtat
    22741 ctctttttct ttttgtttga aacatacaga aacatctcgc aattcaatca ttaaaattcc
    22801 tccttatcta aaaaaagcgt cctcaagaaa atgaaaatgt cattttcttg aggacgcttt
    22861 tttgcgtgtt accacctcat gttcaagctg tgttcacaca caactcctct tcaagtacga
    22921 tcatactcta gcaagatatc ggtcgcagcc gtcatcgcct tacatctatg ctcgggatga
    22981 attgctcgat ggccattttc caatagtcgt tccttgcttc ttctcagcta ccgaagctct
    23041 ctgtcaaagg ttcttctatc gtacttacca tttcaatgca aataatattc aaaaaaaaat
    23101 ccttttacta acataaacta tgtcagtaaa aggacgacgt atcgcggtac cacctttttt
    23161 cggaagtttc cttccctcaa gcccaacatc tcaaaaagag agattggcgt tcgttttaac
    23221 ggtcgaacag ttccgaattt gcctacttat cgacaaatta ttctccaagg ccattttcag
    23281 cttttcttac ttgactcttc tcaccagcca gagcctctct gcaaagtttc caaacttact
    23341 ttccttttca acgaaatcga tattttgttg ttgggattaa tctacatgag gaaaactaaa
    23401 aagtcaagca cgaaattcaa attatttttt ctatatttat ttttctgttt ttttatctaa
    23461 catttcaata acagttcgaa gatcttttcc ttcagcaaga agtttcaatt tttccttttc
    23521 ttcgacggta taatttcgga caaaactgga atcaggtatc aaaacaacgc ctaatcctgc
    23581 tcgtgaagca gctgttgctc ctactaaaga atcttccact gcaaaggctg aactagcttc
    23641 taatcctgtt cgttccaaag ctgtcagata aggaatcggt gaaggcttgt tctccgacac
    23701 ttcgtctccg aatgtaaacg tgtcaaagta atcagctaat ttaaagtgat ccaagatatc
    23761 tgttgctctt ttcgtaacag tggtcgtagc aatgcctagt ttcatatgac gttcttttaa
    23821 gtctgccaat atttcttttg catatggttt taagtggatg gctccgcttg tcagctgttg
    23881 ataaaaatat tcttcacgct tcgctcggac ttcctgtact ttttcagggc tacctgctgc
    23941 tttcactaaa atatcaaaac tttgtttcca gttttttccg ccccaagcgt caacgatttc
    24001 ttttgggata tggatatcaa attgctgaaa tccccaaagc cagccatcac gatacgtttt
    24061 ttctgtgtca actaacaaac catctaaatc aaatattgca cctttcatct atacattaat
    24121 cctttccaaa acaatacaca tagtttagca cgactggtcc aaaaataaca gaagcaaaga
    24181 accagccttc tcgtttataa gagttcttgg aatcgatgga tatagatttt cttcacagct
    24241 gtcacaagcg ccagataagc gatgactgta gcaaataacc ataaccagta attgccagga
    24301 agtggcatca agcctaattc agcaccaaaa cttgtaaaag gaaggacaga accgacaata
    24361 attcctaatg tagtgataac aaacatggca aatgttacgt tgctttggat aaatggtact
    24421 ttcggtgtac gcagtgcatg gagcactaag gtttgtgtcc atagtgattc gacaaaccat
    24481 cctgcatgga ataatgcgat gaaaatcact tgctgtgaac tatccaatgt atggaaactt
    24541 ccgtcaacta cagcaggaca aataacaaaa tacatcaaca agtaagtagt aatatcaaat
    24601 atcgaacttg ttgggccaaa gtaaaccatg aatttcccaa tactagatgc attccatttt
    24661 tttggctcgt ccaaatattc tttatccatc cgatcccatg gcaaggaaat gcaggaaaca
    24721 tcatagatta gattcaaaaa cagaagttgt aaaggcagca ttggtaaaaa tggcaggaac
    24781 atacttgcaa tcagcacgga aaacatattg ccaaaattag aacttgctgt agccttgata
    24841 tatttcatga tattcccaaa agtttctcgt ccagaaagaa ggcctctttc caaaatcgtc
    24901 aaatcttttt ctaaaaggat cacgtctgca gattctttcg caatatccac agctgtatct
    24961 acagaaatac cgatatcagc cactttcatt gcaggagcgt cattgatacc atctcctaaa
    25021 aatccgacag tatgaccatt tttacgtaaa gctgtcgtca aacgtgcttt ttgctgagga
    25081 ttcaatttta cgaatacttc atggtcttca gctacttgtg ctaattcatg atcactcatt
    25141 tccgagattt tctcaccggt aaccaattca ttgatctcta gacctacttg acggcacact
    25201 gaacgtgtaa ctaattcgtt gtccccagtc agaactttta cagctacacc atgttctttc
    25261 agagctttca acgcttgttt cgttgtttct tttggtggat caaggaaagt aagataacca
    25321 atcaaaacca tgtctgactc atctttgact gaaaattctc caacgacgct tggattggtc
    25381 ttttgtgcga caccaatcac tcgtaagcca tcttcattaa gtgactgtac ttttgcaagt
    25441 acggcttctt ttcgttcttt tgttaatggt gtaacagtac cgccaagatc aataaattta
    25501 gaaatgttca gcatttcttc aatcgcgcct tttgtgatca tttgtgtttt accagctgga
    25561 tcagcaacca caacactcat acgacgacgt tcaaaatcaa aaggaatttc atccactttt
    25621 tgataattga tcttatctgt atctaaagtt tctgttgctt catcaatgat agccacatct
    25681 aacaaatttt tcagccctgt ttgatagtag ctatttagat acgcatggcg caaaacacga
    25741 tcatcttcat ttccttcaca atccatatga tattcaagaa tgattttatc ttgtgtcaat
    25801 gtccccgttt tatccgtgca taacacatcg attgcaccaa agttttggat ggcattcaaa
    25861 tttttgatca ctgttccttt tttggccatc acacttgctc ctttgaccaa attcgtcgta
    25921 acgatcattg gaagcatttc aggtgttaga ccaactgcta cagatagtcc aaaaagaaaa
    25981 gcttctagcc aatccccttt tgtaagccca ttgatgacga taactgctgg agccatcaat
    26041 gacataaatt tgatcagtag catggatgtt ttatggatgc ctaattcaaa gcttgattga
    26101 attggttttt cagatagcgt ttctgctaca tgaccaaata gtgtttcatt tccaacagaa
    26161 acaatgattc cttccgcact tccacttatg acattacttc ccataaagac aagattttcg
    26221 taacttgttt cgctttgctg atcgtcttca catactttcg catgcttttc tactggatag
    26281 ctttcccctg tcaatgctgc ttgggaaatg aataaatcct ttgcggacaa taaacgcata
    26341 tccgctggta tcatatcccc tgctgaaagc cgaacaagat ctccacagac gatttcttcc
    26401 ataggaatct ctgtgtactt tccattacgt tttacagctg ccgttacttt cacaagtgat
    26461 ttcagttttt cagccgcttg attggaacgt acggactgaa tcaacgtcat tgtcccgctg
    26521 acgatgacca tagtgaagat aatggctaca ccgaaaagat ctttatctcc aggagctgct
    26581 aatacatatt ccgtgatgaa tgaaataatt cctaatgcga tcaacactaa tgtaaatggc
    26641 gtgatgtatg ctttcaatat ttctacgatg aatggtgttt ttttgccata tgaaatcgca
    26701 ttttcaccgt aattttctct tgaacgttcg atttgttctt ctgtcaatcc atttgctgat
    26761 gcgtggaata gttcaaataa ttcttgtttc gtttttccag caaaagctgc atagattttt
    26821 tgtgtatctt tctttttttc catctcaatc tctcctttgt gtccaaaaga cagctgttct
    26881 cgctgctttt ggtctttagc tattcatcgg atcgatagag aaaaaactga aaggggctga
    26941 gaacagatag ataatagcct tggcctttcc tctagatcct tgtttttcga atgactgtca
    27001 tccatttgtt ctcatctcct ttactaagta attttaattc atgacttcca atcatttttc
    27061 gaatagttat tttcccaatt agaccacctc ctttttattt ttcaccgatg gggatttgcg
    27121 gcaacaaaaa aacctctcgc caaatactca gcgaaaggtt tgaaaaagca tattaaaaaa
    27181 agcttctcag atctcatcgt tgagttttga cactgtacaa cgtaaaaagc agatacctgc
    27241 ttttagctac cttatgaaaa gccttatctc gacaattcct gttctaccca ttggcgtctc
    27301 tcgacgtttt tgggcagtag cctatgtttg tataggagcc tcacctaaca aatcttattc
    27361 ggttttcctt cgttaatata acaactaaaa aagaggatgt caaatcaatt ttccatgttt
    27421 ttcatacttt ttttaaaata cacacgttat attcctgtct cacgcggttt tatttgtctc
    27481 attttccacc tcccgctata atagaaatat agtaggaaac gaggtgctgt tatggaaaga
    27541 acacttgtta ttatcaagcc agacggcgtg agacgccatt tagtcggaag catcattcaa
    27601 cgttttgaag caaagggact ggccattgct gaaatgaagt ttgaaactat gactcctgaa
    27661 ttagcaaaag aacattatca gcacttgact gagcgcagtt tttttgatga actgatcgat
    27721 tacatgactt ccggtcctgt cgtttatctt gtgctggtcg gcgaggaagt gatcgatatc
    27781 gtccgtaaga tggttggggc aacaaaagct gtcgatgctg ttccaggtac gatccgagga
    27841 gactacgcgc tgccggggac tgaaaacatc attcacgctt ctgattcccg tgatgctgcg
    27901 gtaaaagaga tcgcccgctt tttcccgcca ttcgatataa acaaaccgtc tgaaaaagcg
    27961 gcgaagtgat acaaattcta aactgctggt ctagttgctg ggttcagcaa tggccagcag
    28021 ttttttctct aaggacctcc gttgattctt tcctaacata tattcgagca ctgtgaagaa
    28081 ttcttggtag tctttcgtct ttttttcccc taaagcagca gaaatcaatc tttgataata
    28141 atctgccatc aatgtttcat agatcactgt acttctttct ttatgtgcta agctccgttt
    28201 catatcttca ttcacactac gtgccttttt caaccgacca ttttctatat aaaacgaagt
    28261 caggttcatt aaaaaagtaa agagatactg ctcatgattg ttcaactgtg ctaatcgtcg
    28321 cattcttttt ttgattttca aataattgtt ctcaatgaac cgatcactga atatccaagg
    28381 aaaatcagct agaatagata gttcaaattg accatagtct ggtatctgca tcaagtattc
    28441 ttcaatgatt cggcattcat aacgtaaaaa ttcaaattct gtctcagata cttctgtttt
    28501 ttcgaccatg tgaagactca aaaccaaata atacagatct tctgttttct taaaatgcct
    28561 acggttattg ctgatctctt tataaggatc aaagctgtct tcttgtaccc gatctagcat
    28621 tcgtcttttt ttctttaact ttattggttc atccagctgg tttcgcatcc attcatattc
    28681 tgcaagtgtc atgttcattc gctctaaaaa tgatctcaat gttacaaatg acattttttt
    28741 cactgaattt gtctcaatcc cgactagaga agtcttagaa cagattcctt ccgctaattg
    28801 ggactgtgtc aacttcttgt tgactcgaag tttcttgatc aattcaccat catacataat
    28861 agttctcctt tttaatttaa atttttccac attcaataaa tagatacgtt aaaaaggaaa
    28921 aatacacgaa aaaaataaaa aaatgttttt ttcataaaaa ataccaacct tcaagcatta
    28981 gaatgttgtc taactactga aggttggttt gattgtttat tgattatctt tttgctgttc
    29041 aacgtctgtt cttgttactg gatataagtt ggttgttgtt ttattgccat tttttgaaaa
    29101 gacacttgtc gaatgttcgc ctttttgctt ttcgatggct atgttacgtt ctgcttcatt
    29161 tttatagtca tattttttcg gatcgatcgt ttcaaatccg tttggcacat aaaaacgcaa
    29221 gagattttct tgattcagct tatctgaaag acttaaagca gatttgactt tttcctgatc
    29281 tttggaaatt tcctcgtcga ttcctgctgc tttcgtatcg atgatttctc ctgtttggtt
    29341 attgtaagct tttcctccta agatggtgta tttgggtgtc acgaagttcc catttcgaaa
    29401 agccactact tgatcatgct gaggagataa taaatccgta ccaaaatgga gatagtcttt
    29461 atcatcgatt cccaagagat gaagtaatgt cggcaaaaca tcgatttctc caccgtattc
    29521 gtgattgact gaccctttag tataaccagg tatatgaatc attaatggca cacgctgcat
    29581 ttgtgcgttg tcaaattcat tccatgtatc tgggtctttt ccaagagcac ttgctaagtc
    29641 tttattatct gtatttgaga tgccaaaatg atccccataa atcacaaaga tcgaattttg
    29701 gtaaatagcg gattttttca ggtacgtaaa aaattgttcc aatgactgat ccaaataatg
    29761 tgccgttcga acatagttat ctactgtgct atttccagtg ttcaaagatg gaaaatcgaa
    29821 attcttatca tccgtataat aaggcgtgtg attggttacg gaaaggaatt tcgtgtagaa
    29881 cggctgttgt agatgttcaa gatattgggc agattcactg aataaatctt tatctaatat
    29941 tccataacct agcgtttcat ctgattcatc aaaataagat cgatcaaaga aattgtcata
    30001 acctaagttt ttatatacat gatctctatt ccaaaaacta gctacattcc catggaaaac
    30061 agcgcttgta tagccttgct tttgtccaag gatcgctggt gcggcttgaa agacattatc
    30121 tgacccaagt tctgtgaata atgagccttg aggtaaacca aatgtgccag tttccaacat
    30181 attttcagca tcacttgtct ttccctgtcc tacttcatga aaaaaattat caaagctgat
    30241 cgttgcttga ttctggaaaa tactatttaa aaacggtgtg acttcttgtc cgtctacctt
    30301 catattaatc aaaaactgct ggaaactttc taaatggaga acgatcacat ttttcccttt
    30361 agcgatccca aatgtttcgg gatttggctc tgcataatgt ttctttgtat aatctaaaac
    30421 ttcatcaatc ccattactgc ttgcatgcgc gcgaacactg ctagtcattc ctgttttgat
    30481 tccatcataa atagtatacg catcgattcc tagatatttg acaatatatg aacggtcaaa
    30541 tgttcgctgc agcaattgtg gacggttggc ttcgctcagc gctagatttc ctgaaaaaat
    30601 aagccctgaa agacaggtca cagcgatagc catcggtttg ctgacaggtt gacttttgat
    30661 aggtattttc ttgatcgtca accaaaccag tatcccaatg aacgtagcaa tatccagcca
    30721 gtagaggaca tcatggggtt tcatcaaaga aaaggagctt cctgataacc cttgcgacac
    30781 tttcgaaaat cccaatacag atttgactgt aatgaaatct gtgaattccc gataaaaaat
    30841 aatgttcaag tacaacaaaa gcgtgtttaa aatatcaatg atcaaaagaa tggctaatgc
    30901 tggctttggc tttttaatat acaaagaaag gccaaaaaac aataaggtag ttgcaatcgg
    30961 gttgatccac aagataaagt actggatcgt accttccaca cctaacgaaa aatctgcata
    31021 gtaagcaata atcgtcttca tccagaaaaa gaaaacagct gtcaaaacga ttgctaaacg
    31081 attttggcac aattttttaa gttgtgtcac gtatatcact tctctctatt cacatttttc
    31141 cttattttgc tcctgaaaaa tgaaaattcc ctaaaaaagt tgttatctta agattttttt
    31201 attctttcta aagattattc cgtgcacttt tcacagcata aaaccgctgt gatataagcg
    31261 attgaaacag taaggtgata aaagtcttgt cgcagctcct gtttagttga tgcaggacag
    31321 ctttttcaca acttcttctt taaacggtgt tctcctgcca actcctcggc cacaagtcac
    31381 aatatttata aaacatgaga agctgttttt tgaaattgtt ccttgtggct cagagttaaa
    31441 cggcaagctc aagacctctt gata
//
LOCUS       DACDYJ010000028        31426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDYJ010000028 DACDYJ010000000
VERSION     DACDYJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.028"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            261..1130
                     /locus_tag="IWM51_001315"
     CDS             261..1130
                     /locus_tag="IWM51_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP9970744.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1339..2364
                     /locus_tag="IWM51_001316"
     CDS             1339..2364
                     /locus_tag="IWM51_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9970745.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2403..3353
                     /gene="manA"
                     /locus_tag="IWM51_001317"
     CDS             2403..3353
                     /gene="manA"
                     /locus_tag="IWM51_001317"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP9970746.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3447..5150
                     /locus_tag="IWM51_001318"
     CDS             3447..5150
                     /locus_tag="IWM51_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9970747.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5152..7185
                     /locus_tag="IWM51_001319"
     CDS             5152..7185
                     /locus_tag="IWM51_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9970748.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEEPMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7196..7636
                     /locus_tag="IWM51_001320"
     CDS             7196..7636
                     /locus_tag="IWM51_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9970749.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7836..8963)
                     /locus_tag="IWM51_001321"
     CDS             complement(7836..8963)
                     /locus_tag="IWM51_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9970750.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8966..9643)
                     /locus_tag="IWM51_001322"
     CDS             complement(8966..9643)
                     /locus_tag="IWM51_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9970751.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9907..11673
                     /locus_tag="IWM51_001323"
     CDS             9907..11673
                     /locus_tag="IWM51_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP9970752.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11688..12125
                     /locus_tag="IWM51_001324"
     CDS             11688..12125
                     /locus_tag="IWM51_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9970753.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12138..13289
                     /locus_tag="IWM51_001325"
     CDS             12138..13289
                     /locus_tag="IWM51_001325"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP9970754.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13351..14049)
                     /locus_tag="IWM51_001326"
     CDS             complement(13351..14049)
                     /locus_tag="IWM51_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP9970755.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14042..15394)
                     /locus_tag="IWM51_001327"
     CDS             complement(14042..15394)
                     /locus_tag="IWM51_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP9970756.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15617..17017)
                     /locus_tag="IWM51_001328"
     CDS             complement(15617..17017)
                     /locus_tag="IWM51_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP9970757.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17178..17915
                     /locus_tag="IWM51_001329"
     CDS             17178..17915
                     /locus_tag="IWM51_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP9970758.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18040..18519
                     /locus_tag="IWM51_001330"
     CDS             18040..18519
                     /locus_tag="IWM51_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP9970759.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18516..19283
                     /locus_tag="IWM51_001331"
     CDS             18516..19283
                     /locus_tag="IWM51_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970760.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19513..20082
                     /gene="lepB"
                     /locus_tag="IWM51_001332"
     CDS             19513..20082
                     /gene="lepB"
                     /locus_tag="IWM51_001332"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9970761.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20186..20788)
                     /locus_tag="IWM51_001333"
     CDS             complement(20186..20788)
                     /locus_tag="IWM51_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9970762.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20894..21363
                     /locus_tag="IWM51_001334"
                     /pseudo
     CDS             20894..21363
                     /locus_tag="IWM51_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21469..22053
                     /locus_tag="IWM51_001335"
     CDS             21469..22053
                     /locus_tag="IWM51_001335"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9970763.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22056..23129
                     /gene="prfA"
                     /locus_tag="IWM51_001336"
     CDS             22056..23129
                     /gene="prfA"
                     /locus_tag="IWM51_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP9970764.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23122..23961
                     /gene="prmC"
                     /locus_tag="IWM51_001337"
     CDS             23122..23961
                     /gene="prmC"
                     /locus_tag="IWM51_001337"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP9970765.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23954..24133
                     /locus_tag="IWM51_001338"
     CDS             23954..24133
                     /locus_tag="IWM51_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970766.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24155..25180
                     /locus_tag="IWM51_001339"
     CDS             24155..25180
                     /locus_tag="IWM51_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP9970767.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25355..26599
                     /locus_tag="IWM51_001340"
     CDS             25355..26599
                     /locus_tag="IWM51_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP9970768.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26752..27699
                     /locus_tag="IWM51_001341"
     CDS             26752..27699
                     /locus_tag="IWM51_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9970769.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27833..28462
                     /gene="upp"
                     /locus_tag="IWM51_001342"
     CDS             27833..28462
                     /gene="upp"
                     /locus_tag="IWM51_001342"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP9970770.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28601..28843)
                     /locus_tag="IWM51_001343"
     CDS             complement(28601..28843)
                     /locus_tag="IWM51_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970771.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28964..29036
                     /locus_tag="IWM51_001344"
     tRNA            28964..29036
                     /locus_tag="IWM51_001344"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28997..28999,aa:Thr,seq:cgt)
     gene            complement(29155..29793)
                     /locus_tag="IWM51_001345"
     CDS             complement(29155..29793)
                     /locus_tag="IWM51_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9970772.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29916..30548)
                     /locus_tag="IWM51_001346"
     CDS             complement(29916..30548)
                     /locus_tag="IWM51_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9970773.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30899..31198
                     /locus_tag="IWM51_001347"
     CDS             30899..31198
                     /locus_tag="IWM51_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9970774.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        10719 a         5482 c         6319 g         8906 t
ORIGIN      
        1 tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatag gaggagctat
       61 ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc tctttttata
      121 agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct cacatttttt
      181 ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa taaagcttga
      241 cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc tatttaaaaa
      301 gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag aacgttgttg
      361 gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa gaagctggaa
      421 gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg gtgatcaaaa
      481 agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat atcctttgtc
      541 gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga cgtatgggga
      601 ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag aataagaacg
      661 accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa tctgaagaaa
      721 aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat gttactaact
      781 taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat aatgaagaag
      841 agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa gctagcggag
      901 gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa ttcatgatcg
      961 atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt gatttcttct
     1021 tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa tggtggttcg
     1081 tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa ctttttcttt
     1141 cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc ttattctaga
     1201 gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc agtttttttg
     1261 tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa gaatctgata
     1321 gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa aaagatggaa
     1381 gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt agaaacattt
     1441 acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga tctgttgttt
     1501 gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg tctattagaa
     1561 gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt tgaaaatcag
     1621 acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg gataccaaac
     1681 agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga accaggtgag
     1741 caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg gttgatgcta
     1801 gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt ggaaccatta
     1861 ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc cacagaacaa
     1921 ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag agttttggct
     1981 gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac ttttgtccaa
     2041 atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga gacgattgat
     2101 tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg atgggactta
     2161 aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac aggtacgttt
     2221 ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg cgtacatcct
     2281 gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa ctttttgaaa
     2341 gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga ggagtttgaa
     2401 ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg ggaggaagtc
     2461 gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa gattgggcga
     2521 tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa gggaaaagat
     2581 tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa ccagtgtttc
     2641 cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta catccagatg
     2701 atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg tatatcatcg
     2761 atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga gaagaactag
     2821 ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca gttaaaaaag
     2881 gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga atcatgattt
     2941 tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac agaaaagatg
     3001 cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca actgtccctg
     3061 cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg atcgtcactt
     3121 atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc acatctttca
     3181 aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac cttgtcgttg
     3241 acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat ggcgtttctg
     3301 agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa tagttcccag
     3361 atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa atccgtccat
     3421 caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac aacagcagtc
     3481 gcgtgtctat gaaacaactg agaaaagctg gacatcactt aaatgaaatg atcatgccaa
     3541 atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt gtgtctggcg
     3601 atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt ttacccatct
     3661 tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc gccggtgcaa
     3721 ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga gcgatgatta
     3781 tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat gtgaaattca
     3841 aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt agcgttattt
     3901 gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg ttgctcgcct
     3961 ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg atcttagaac
     4021 ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca cctttaggaa
     4081 tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca aatcctggac
     4141 ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa aaagtaaatg
     4201 cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat tttccatttg
     4261 tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc ggcacgttga
     4321 tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca ctactcgtga
     4381 tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata gcaatcagta
     4441 catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa gaaaaagaga
     4501 acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat tccgtcattt
     4561 ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg aaaaagaaat
     4621 tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa ttgaataacc
     4681 aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga gaaaaagtac
     4741 ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg ctgtcacgaa
     4801 taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca gatatcacag
     4861 aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga cgcggctcac
     4921 aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa cagtttgtta
     4981 tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat atcgcacaaa
     5041 gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt ctagttgaac
     5101 attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa gatgtattta
     5161 tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc tgtatctatc
     5221 caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga attagataat
     5281 cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag aggctcattt
     5341 gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga tgggaaaagt
     5401 aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt actgacgaca
     5461 gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac cactttttat
     5521 gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat cattcggaac
     5581 cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc gaatgtatta
     5641 gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc atcactgaat
     5701 tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt aatcggggaa
     5761 gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt ggttttgatg
     5821 ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga gacatataat
     5881 agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg gatcgcttct
     5941 gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt attaaatgac
     6001 ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca gcttgcttat
     6061 ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt ttatgaagat
     6121 gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag agccgtttta
     6181 caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata cgaggaagtt
     6241 gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt atccgaagaa
     6301 gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc gaaagaaagc
     6361 aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat gttggaacta
     6421 cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat tgctgattta
     6481 aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat tttaccagga
     6541 tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt caagcagcta
     6601 aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta taacagcaat
     6661 aacgagtgga tgatagaaga tacttatgag gaaccaatgc atttcatgga tcaaatcaat
     6721 caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat ttccgaaacg
     6781 attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga ggtggttcat
     6841 cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac aaatatgggg
     6901 ttattccata catccagctc attgatttct aaaccgatct tttgtatttt caatttgaaa
     6961 catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg tatgttagtt
     7021 atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa gatcagtgga
     7081 gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa agaaatcgtt
     7141 tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg aaaagatgat
     7201 acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga aagaagcgtt
     7261 gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat atatccaatc
     7321 aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag ccttgccaca
     7381 tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc aagtgcctga
     7441 tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat ttgtagtggc
     7501 tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct gttcagacat
     7561 cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa tgatttttga
     7621 acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc aagtaataac
     7681 ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat tactgcggat
     7741 gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg ggacggtgac
     7801 aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt tatatagata
     7861 gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa gataccattc
     7921 atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat gatcacattt
     7981 tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca tccaatcgaa
     8041 gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag attgactaga
     8101 gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag cacccacaac
     8161 aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg tatctcctga
     8221 ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat tattactagt
     8281 atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata ataaatggta
     8341 aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag agagattacc
     8401 tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat acataaaatc
     8461 aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa agagagatgc
     8521 gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata acggagcaac
     8581 tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga aatcagtcga
     8641 tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac attccctgtg
     8701 ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact ttccacaaat
     8761 tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc ttcatagtta
     8821 tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag tgggaatgca
     8881 aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag taagagattg
     8941 cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa atagctaaaa
     9001 tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa catgctttag
     9061 aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc ttcccctctt
     9121 tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct aatccgttca
     9181 acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca agcaagacat
     9241 gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca gtcatcttca
     9301 atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg atcaaagtca
     9361 aataatcaac agccgataga tagtcggata actgattcgt tgattctaga aaaaagaaag
     9421 accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat tgatcataag
     9481 ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat ccgttcgggg
     9541 caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga atcacacgtt
     9601 ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg cctccaatat
     9661 aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca aaagttattg
     9721 tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt tttctgatac
     9781 cgaaatcttt ttattttgct gacatactca aacctttggc acaaagtttc gtgccaaagt
     9841 acgactattt ctctttttag taagcggata caattagagt atcaaaagga gagagggaga
     9901 gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag ggtacaaaaa
     9961 ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat tgcctggggt
    10021 gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt cgctaccatg
    10081 gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg cggaaaagta
    10141 attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt gattgtcgga
    10201 acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg atatgccatc
    10261 aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat gctagtcaat
    10321 aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc tgcgattggt
    10381 ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat tttatctgcc
    10441 catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt tttgaataat
    10501 gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga ggctggaaaa
    10561 tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt acttgctttt
    10621 actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat cattcacttt
    10681 ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt attattctta
    10741 tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga tgctggacta
    10801 agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc gttcaattct
    10861 taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg tatttctgca
    10921 gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc tgttcaagag
    10981 acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg agctacattc
    11041 tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag tgcgatgggc
    11101 gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt gaccaatcaa
    11161 gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt cgaactacaa
    11221 gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa ctttttgaat
    11281 tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac atcagtttct
    11341 gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca aatcaaagag
    11401 attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac cgtgctcaaa
    11461 gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat ccaagattta
    11521 aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa agtacagatg
    11581 gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca aaaatacgaa
    11641 tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg caaagtttgc
    11701 cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg atccgctatt
    11761 gcggtcagat gcttgtagaa gcagggtgcg ttgatgagga atatatcgat gccatggtag
    11821 atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct catggaacag
    11881 atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta ccagatggtg
    11941 tagattttgg taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt attgcaggag
    12001 tcggagatga gcatttgcaa cttgtacagc aaatcgcact ttattgcagt gatatggaca
    12061 atgttgtcca gctagcagac gcgctcagca aagaagaagt aacggaaaat ctagcaattg
    12121 cttaaggaga gagaaaaatg agagcagtac attttggtgc aggaaatatc ggtcgaggat
    12181 ttattggaga ggttttggca gctaatggat ttgagattac tttcgtagat gtgaatcaca
    12241 cgatcattga tgctttaaat gaaagaaaag aatacgaaat cgaattagca gatgaatcaa
    12301 aagaaaaaat cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat ccagaaaaag
    12361 taatccaaga aatcgctgaa gcagatttag ttactaccgc cattggcccg aatatcttac
    12421 cttttattgc tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa ggaagcacaa
    12481 catctttaga tatcattgcc tgtgaaaata tgattggcgg ttcagcattt ctagaaaaag
    12541 aagtgtataa atatcttccg gaaacatcat ttactgacaa atatatcggc tttccaaatg
    12601 cagcagtcga tcgaatcgtc ccattacaac accatgaaga tcctttattc gtccaagtgg
    12661 aacctttcaa agaatgggtc attgacgata gccaaagaaa aaataaaaaa atccaattaa
    12721 aaggggtttt atatgtagat gatctagaac catatatcga acgcaaacta tttagtgtca
    12781 atactggtca tgcgactgtc gcttatacag gtgccttgtt aggttaccag accattgatg
    12841 aagcaatgca agatgcgtta gttgtggcac aattaaaatc agttttgcag gaaacaggaa
    12901 gtttgctgat tgccaaatgg gggtttgatg cagagcagca tcatgcgtat attgaaaaaa
    12961 tcattcatcg cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc gctcggacac
    13021 cattacgtaa gttaggttat caagaaagat ttacacgtcc agtgagagaa ctacaagaac
    13081 ataacttgac ttgcccacat ttgacggcaa caatggggat tatttttaat tactatgatc
    13141 ctgaagatga acaaagccgt caattgcatg aaatgaagat ccatgaaaac ttggaacaat
    13201 tgattcaaga agtcacagga atcaatgatc cgaaaacaat cgggaatatc aagcaaaacg
    13261 tgaaccgtta tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga atgtgacgaa
    13321 atttttgtca cattcccctt tttcgaataa ctaggctgtt tgtccttcaa gatagggcag
    13381 atccaattct ttaccgtaac gttgttctaa agctaggcgg atcaaacgag ctgccaggtt
    13441 ctcatttctt gcaaggatag gtccatggaa ataagaaccg aagacatttc ggtaaatgac
    13501 accttcagat tggtcttcac cattatttcc ttgaccttgt accactttcc ccaaaggtcg
    13561 ttcaccttca cctaagaaag tccgaccatt gtggttttca aatccataat aagtttcatt
    13621 gaattcttcg ttatggataa cgatatcacc gataaaccgg ttattttcct gacttaacgt
    13681 gtagtgatcc agtgcactga ttccttggat cttttctcca ttagctccca tgtagtagtg
    13741 acctagcaat tggtagccac cacaaatggc taacatgacg ccatcatttt cgatatagtt
    13801 gatcaaagaa tctttctttt tctggatgtc ttttgaaatg attaattgct caaagtcttg
    13861 accaccgcca aagaaaacca agtcatattt atcagcatca aaaggttcat gtatactaac
    13921 gatctccgta tggcaagaaa cccccatttt ttctgctata tatttcaaca tcaaaaggtt
    13981 tccgttatca ccatatgtgt tcatcaaatt tccataaagg tgggcgatat tgagttcata
    14041 attagccacg gttcattcct cctttgataa atccttgagc agctagttct ttacgaagct
    14101 gcaatacagc agtgtaagta gctaaaatat agacatgctc agttggcaat tctttgattt
    14161 tttcgattac ttctggtaat tcttttgttt ctgtcaattt attttctgga atcccggcaa
    14221 cttttagacg taaagtcata tctgtatgac ggtctcctcc agaaataaca gctgggatat
    14281 ccatatcggc aaagttttca aaattaccat cccaaatcca gctgacatcg attccgtctg
    14341 cgtaattggc atttaataaa gccactaaag aaaatggata aggagctaga ccaatcatat
    14401 cgattacttg attgatacct acaggatttt tgacaagtac taatgtacat tttttcccat
    14461 tgatattgat gacttcttgt cggccgaata ctttctcgtc atatcctaaa ccttgtttga
    14521 ttttatcagg atcgacattg aagtactctg ccactgcagt agctgctaaa gcattgtaga
    14581 cattatacat accgccaact tcgattccgt attcatgacc atcgatcacg aaatctgctg
    14641 acacattgtc catccgtacc atatcagtta atcgataatc caattcagga cgtttgaagt
    14701 cacaatttgg acagtaatat ttccctaagt ttgcataagt gatcatttta taatgcaaga
    14761 tgtgatgaca atttggacat aagacaccgt ctgtattgta atgagccatt tgttcacgat
    14821 cttcttcatg gtcaaagcca taatattttc taggattgac tgtttcgata gaattgaaga
    14881 ttggtgaatc accgttactg ataatcgttg catttggtga ctttgcggca ccgtctacaa
    14941 tcattttata ggtcgtatag atttctccat accgatccat ttgatcacgg aaaatatttg
    15001 taaataagaa cagttctggt ttcatatatt ctgtgacttt gctcaaactt gcttcatcta
    15061 tttctaggac agcaaacttt ttctgtcctt tttttggttt tgcctgcaag aaagtcgata
    15121 caattccctg taccatattc gcaccagtag ggttggttag aacttcatcg aattgctggc
    15181 gcaaaatatt gactgttaaa gcggtagtca atgttttacc gtttgttccg gtgacaacga
    15241 cgacttggta gtcttttgcc aaactatcta gtatatgtgg atcgactttc agcgctagtt
    15301 tacctggtaa acttgatccg cctttaaaaa aagtttttaa tgcccattgc gacgtttttc
    15361 caactgcaat cgcaaaatga ctgcgtattc ccattatgta acctccaaaa acgatttgct
    15421 gacgaataaa ttcaactaat cataccatat gttgaaaaaa aggttaaatt gcttttctta
    15481 ttcttaagaa aaggttacgg ctgaaagtgc atgaaatcgg acaaaatcgc atgatttaac
    15541 aaaaaaacag cttgaaacaa aatgattgtt tcaagctgct gtgattttat tattccctct
    15601 tacctatatt cccattttaa ccgataataa ttttctcgct tggatattca tagcctagat
    15661 ctcgtgtttc ttttggcacg aataacatca atgtatatag cattccgatc cgcccaataa
    15721 acatcagtgt agcaatcgtg attttgccca caacagacaa gtctccggta atccctaaag
    15781 ataaaccagt tgttccaaac gcagaagtta cttcgatgat gatcgagatc agcgtttgtt
    15841 cttctgtagc agataaaaaa acgatacaaa agaaacacat accaagagaa agcataaaga
    15901 caacgactga ttttcgcaca tcatcttgat cgattctccg tccaaaaata ttgatgttat
    15961 cttcactttt gaggaaggaa tatagataca aaccgatgat tgctacagta gttgttcgaa
    16021 tcccgccgcc tacagaacta ggactgcatc cgataaacat cagtaaagaa aaaatgatca
    16081 gtgttgtgat ttgaaaatca cccaaatcgt gaatctgcag accagcatta cgagtcgtaa
    16141 tcgaataaaa catcgaattg atccatttga cactcatatt ggaatcttgg aataaatggt
    16201 ccttttccaa tagataaatc aagacagttc ccgaaacaaa caagataaca aaagctaaca
    16261 cagctaattt ggtaaacaag gaaaaacgaa acggaagctt cgcattggaa cgtttgtata
    16321 atagccattc ccggcactcc atcaatacag gaaaaccaat tcctccgaca aaaatcaaaa
    16381 acatgatcag gatcaagaaa aaatagtcat gtgcaaaggg cttgatggaa tccccagtga
    16441 cgtcaaaccc agagttcgtg acagccgata tcgcctgata aaaaccgtaa aaaacggcat
    16501 cacgccaacg atcaaaatag cctctgtaat aaaaataaat agaaaagaat gtcccaaaaa
    16561 gtaattgaaa ccagattaaa atagcaaaag tgatccgaat cagtcggacg ataccagaca
    16621 aacgcggttg attcatatcc gtcatgatca gctgtcgctg tttcagtgtg atccgtcgtt
    16681 tagaaaaaat gacaaaagca gtagaaatca tcatgatccc caaaccacca acttgaaata
    16741 atatttccag cagtataata ccgttatcat tgaacacaga attgatatca aaagtcgata
    16801 gtccagtcac actcactgta ctgattgcca tgaacagcat gtccacaaaa gaaacatggc
    16861 tatctggttc gcgaaaaatc ggcatataaa atagaacgag tgataagacc gtcatcaata
    16921 tataataaaa aacgatgatt tggatcgatg agaaatgatt ggcagcaaac ttctgtcctc
    16981 gccgctgcag ccttcgaaag aataaatcaa catgcacagt actatcctcc tgacaatagt
    17041 cgtttctacc agtatacaag gggaattagg aatactcaaa gaaattatca aacagataac
    17101 aatacagctt tcgattaaaa tagaaatggc ttataataag gaaaatccta gaaaaaagaa
    17161 ggaggaggaa taacaaattg attaatgctc aacaaatcgt agaaaaaatg aaaaatcaaa
    17221 aaataaatta tgatacagtt ctgaaaaaaa tgatcatgca atgggagaaa gaagaagtaa
    17281 agcctaagat attaatacat acatgctgtg ctccttgcag tacgcacaca ttggaattca
    17341 tgtctcaata tgcagagatc acgttatttt tttcaaattc gaatattcat cctaaaagtg
    17401 agtatttgcg aagattacat gaacaaaaac gatttgtaga ccaatttaat gagaaaactg
    17461 accatcatgt tgcgctaatc gttgatgaat accgtccaaa tgaattcacc aaaatggttt
    17521 taacaaacca tcttgaaaac gaaaaagaag gtggtgctcg ctgcagtgca tgcttcaata
    17581 tgcgattaga tcttgttgcg caaaaagcgc aagaactagg atacgattac tttggaagtg
    17641 ccctgaccat ctcgccaaag aaaaatgcag ctttgatcaa tcagatcgga atggatatcc
    17701 aaaagattta cgggacacaa tacttgccaa gtgatttcaa gaaaaacaaa ggctatgaac
    17761 gctctttgca aatgtgcaaa gagtatgata tttatcgcca atgttattgt ggttgcgtgt
    17821 tcgctgccaa gcagcaagga tgcgatctga aagaaatcaa tcgggaagca aaagcttact
    17881 taaaaacatc aaccgtagtt tttgaggaaa attaaagaaa tctttcggaa aaacgtaaga
    17941 aaaaaataaa gaatttatgg tagaaataag gtagactgta taatttgtcc gtagtaaagt
    18001 aaggaaacaa gaataaattt caatgaagga agagataaga tgcgaaaagt agcacctatt
    18061 ttgaagccaa tcgctgtatt aacaatctcg acgattgtcg cgactactac aggtattgtg
    18121 aatagttcca gctaccgtgc accaagttca agttctagta ctgtagaaga aacaacacca
    18181 actacttcag tctcgacatc tgaaccaaca acaacggaaa gtttgaatga aaccactacg
    18241 acaagtacat gggacgatac aacaactagt tcgtcgtatg atgagacata tacaactgac
    18301 agctcaagcg aaacaagtaa tacggtacca gatggcactc catcggattc aaccaatcca
    18361 agtacaagtc cggatacaag tgctagctcc gaaacgatac caagcagtac gactgtagat
    18421 gataacacgc aaaatgatga tgcagcacgt acagcacagc aaaaccaaga ttcaggaaat
    18481 acccaaacgt ataaccagca agcaggtgat tctcaatgac atttttacta gaccttatca
    18541 acggggtgca taaattcctt ggctatttag acatcagtcc taaatatctt aaccgtggat
    18601 atacgatttt aagtgtgatt cccacgcttt atttattgag aattgtttat ggactttggc
    18661 aaaaccaaaa ctatcttcaa tttttcttat atggacttgc atttctagtg cttgtttatt
    18721 ttactgtttt aaatgtattt tattactttt tagataaaaa cacgaaagcc gatgtcactc
    18781 agctttttgt gaagtattta cctgatgaag cgttcaacat ccagacagaa gaatcgatag
    18841 ataatggaaa ttcgatcgat aaagtgaata cacaagaggt catggtttct tatgacgaag
    18901 attatcaatt gaaattagcc gagaatatgc ggtatttgat tggaaatggt gagatcaaaa
    18961 caaatgaact cggtcgcatg gatggatttc tggtcgatag aaatacattg tatccttatt
    19021 actatgtcaa gaaaacagga gagaaagagt atgttctaaa tattggacga agctactcgg
    19081 acatggaaaa agtagggacc attcgacttg actcaacaga agacacattg gaacctgtcg
    19141 gattgttcat cgtcggcggt gattttgtca aagacgggtt acgctatcat gagccttatc
    19201 gtctgaaatt gattgcaaaa aaggcacagt cgcaggaaga accagtggtt tatagtcgta
    19261 gccaaagaag aagacattca taatgtgatg aaacgccgtg tatcagtggt tgcgataaaa
    19321 acgtttattc ggaataaggg ggatgtgaca cgtcttttgt cacgttcctt ttttatataa
    19381 cgagaaagat aagacgaaca catcacttat tctttccttt catttataaa cattgtagaa
    19441 taaagaaagt acaacgaagt attgtgaaaa aacgtaataa ataacgagga aaatacaagt
    19501 aggaggtaag ctgtgaaaga atttttgaaa aattggggga ttttgatcct tgtattagcg
    19561 gctattttat tagctagagt gtacgtgttt acaccagtta ctgtaaacgg gcattcgatg
    19621 gaccctacat taagtgatgg acaacgattg atttcatcta agatctcaaa ctatgaacga
    19681 atggatatta ttactacaaa ggaaccgggg gatgaagaac ggatgattgt taaacggatc
    19741 atcggtatgc ctggagatac agtcaagatg gaaaatgacc agttgaccat taatggcaaa
    19801 aagtatgatg aaccttatct ggatgagttc aaaaaagaat tttcagaaga taagttacaa
    19861 ggagaatatg cttatagctc aggattccaa gcacaagcag aaagttcatc cacctttacg
    19921 agtgactttg aatatactgt gccaaaagga aagtatcttg ttctaggcga taaccgcttg
    19981 atttcaaaag acagccgaat gtttgggtta gtcgacaaag atatgatcca aggaaaagtt
    20041 gttttccgtt attggcctct ttcagaaatt aaaatcatgt gatgaaaaaa taaaaagaag
    20101 ctgtgacatg ttttttgtca cagcttcttt ttccgaaaat tccttcttat ttcttggagt
    20161 tgctcacttt ttttgcaatc tcattttaag ctttttggct gatggataag aaaagcgaag
    20221 cgtttttgtt cataaatagc ttttccccac gatggaaggt atgactagta gcagagacaa
    20281 agcccgtttt ttccaatcga ttattcaatt cttcttggct aaagccatta tggactttcg
    20341 gatgactaat ttgttcattc ttgtcaaagt ctacgatgat caattgaccg ccaggagcta
    20401 aaatttcata aagttttgta agaatattct ccgtatcagg aatgtgtaaa agtacaagtg
    20461 atagaaggat gagattagct tggacagcag gaaattcgac agaagcatca gcgtggatcg
    20521 ttcggacatt tttcagatct gctgcttgga tcttttcttc tgccattttc agcatagttt
    20581 ctgaagcatc ggcaatgatc agttctttga atctttccgc aagcggtaaa ctaacaagcc
    20641 cagttccgcc tccgtaatcc agcaggactt tttggtggct gtcgcgcggt agataagtag
    20701 ttagttcttc gcgaataatc ttggctaatg cttgacggtc aggggaatca taatggttag
    20761 cgatggaatc aaaaatattt tttgtcatca aatagctcct tacgtaaaac tttgaaattt
    20821 taacttatta tagcacgtga aaagaaagaa agcatgctat actcaattca aaaagggaga
    20881 atctagaaag aatatgccga tatcgtttgt aaaagataga gaggaaaaag gaaagtgtgt
    20941 ccgagaaata ttactagact tgcctgagtg gtttgggctt cctgaatcaa cggaaaaata
    21001 tatcgaggaa tcaagtaaac tacctttatg gtgtgaaaaa agaaaagaag aatatttagg
    21061 ctttatcact ctttcacaga caagtgaaga tactgccgaa atctattcta ttgtatggga
    21121 ataaaaaaag actatcatca ccaaggatta ggaacaaaac tttttaaaga agcagaaggt
    21181 tacgcagcaa aacattacaa atatttgcaa gtgaagactg tagacgaggg gcactactct
    21241 atatatgatc aaactatttg tttctacgaa tcattaggat tttcaaggtt ggaagttttt
    21301 ccaaatttat gggatgaatg gaacccatgt ttagttcttg tcaaaaaatt ggaacaaaag
    21361 tgagcaatag atttaatact aatctgtgct tgatgaaaat tctcactgta aaaaatgctg
    21421 aaaatcaagt ataataccat aggaacaaac tggaaggtga aaaagagcat ggcacaacta
    21481 ttttttaagt atggcgcaat gaatagcggc aagacgatcg agatcctaaa agtcgcacat
    21541 aattatgaag aacaaaataa gccagttgtc ttaatgacaa gcggtattga tacaagagat
    21601 ggtttaggcg ttgtttcaag ccgaatcgga ctgaaacgta atgcgatgcc gatatttgaa
    21661 gaaacagacg tatttgacgt aattcagaaa atggaaagca aaccctattg tgtgctgatc
    21721 gatgaagcgc aattcttaaa aaagaaacat gtcattgctt ttacgagaat tgtggacgaa
    21781 ttggatattc cggttatggc atttggtttg aaaaatgatt ttcgaaacga gttatttgaa
    21841 ggatcaaaat atttattgct ttatgcagac aaaatcgaag aaatgaaaac aatctgttgg
    21901 ttctgccaca aaaaagcaat catgaattta cattatattg atggaaaccc tgtttatgaa
    21961 ggcgatcaag tgcaaattgg cggaaatgaa gcttattacc ccgtttgtcg aaaacattac
    22021 tttcacccat caatgattac agaaggagaa taagcatgta cgatcaatta caagcaattg
    22081 aagaccgtta tgaagagctt ggagaacttt taagtgaccc agaagtgatt tctgatacaa
    22141 agcgttttat gcagctttca aaagaagaag caaacacgcg cgaaacagtc gaagtttatc
    22201 gtcgttataa agaagtagta caaggaatca ccgatacaga agaactgctt ggtgaaaaac
    22261 tggatgatga aatggctgaa ctagcaaaag aagaattatc tgaattaaaa aaagaaaaag
    22321 aagtcttaga agaaaaaatc aagatcttgc ttttaccaaa agatccaaat gacgataaaa
    22381 atatcatcat ggaaatccgt ggagcagcag gcggagacga agcagcatta ttcgctggcg
    22441 atcttttcaa tatgtaccaa aagtatgctg aagcacaagg ctggaaaaca gaagtgatgg
    22501 aagctaacgt taccggaatc ggtggatata aagaagtgat catgatgatc accggagaca
    22561 acgtatattc gaaattaaaa tatgaaagtg gtgcgcaccg cgtccagcgt gttccttcaa
    22621 ctgaatcgca agggcgtatc catacttcca ccgcaacagt tgtcgtgatg ccagaagctg
    22681 aagaagtaga gatcgatatt gaagacaaag atatccgtac agatatctat catgcttcag
    22741 gtgctggcgg acagcacgtc aataaaacag catctgctgt acgtttgact cacttaccaa
    22801 cagggatcgt tgttgctatg caagatgaac gttctcaaat caagaaccgt gaaaaagcaa
    22861 tgaaaatctt gcgtgctcgt gtgtacgata agatccagca agaagcacaa agcgagtatg
    22921 atgccaatcg aaaatcagca gtcggaactg gggatcgttc agaacggatc cggacgtata
    22981 atttcccgca aaatcgtgtg actgatcatc gtatcggatt aactatccaa aaattagatc
    23041 agattttagc agggaaatta gatgagatcg ttgatgcatt gatcatgtat gatcaaacat
    23101 caaaactgga agctatgcaa aatgggtaaa atgacctatc gagaagtcct gtcacgggct
    23161 tcttcttttt tagaggatca tgggaaagaa gcatatagta tccaattttt atttttggaa
    23221 cgaaaacaat ggaaaaaact tgattggctc cttcatatga acgaagagat ttcagaagaa
    23281 gaacagcggt tgatcgaaac cgatctgcga cttctgcttg cagatcatcc acctcagtat
    23341 ttgctaggat atgcagattt ttatgatcat cggttaaagg taactgaagc aacactgatc
    23401 cctcgaccag aaacagagga gttagtcgaa tggtgtttag acgagacgcc tggtgtaccg
    23461 ttagaagtga tagatattgg gacagggact ggagcaattg ctattagttt gaaagcagca
    23521 agaaaaaatt ggcatatatc tgctgtggat ctttcagaag aagcattaga agtagcgaaa
    23581 gaaaatgctc agaaagaagg aacgaaaatc agtttttatc atggagatac attaggacca
    23641 gtaatggatc agcaatttga tgtgattatt tctaatccgc cgtacatcag tcggaatgaa
    23701 tgggaactga tggatgaaag tgtccggtca ttcgaaccta aaatggccct ttttgctgag
    23761 aacgatggat tagctattta tgaaaaaata gcagttgaag caccaagcgt actaaaatca
    23821 aatggaaaga tttttttaga aatcggattt cgccaaggtg aagcagtaaa aaaaatattt
    23881 cagcaagcat ttccagataa aaaagtggcg ataaaaaagg atctttttgg caatgaacga
    23941 atgatccaag tttatggttg aagttgtgga tgaaaaattt tttatttgtt tttttattat
    24001 aaaaaaacac ttatttgaca aggatgtaaa cgactttttg gtttatcaac aagtttatcc
    24061 actattgaca aatagattat ccacaaacag tggaaaactt gaggataaac tcagacaaaa
    24121 taagaatgta tgaaaaaggg gttgtggata aactgtggaa acgaaacgat atgatgaaac
    24181 agaattgcaa gaggcagccg aagcactgaa agaaggagaa cttgttgctt ttcctaccga
    24241 aacagtatac ggattgggcg ccaacgctct tttaccaaac gctgtcaaga aagtgttttc
    24301 tgtaaaaggg agaccgcaag ataatccatt aatcgtccat gtcgcctctt ttgaacaagt
    24361 aaaagagtat gtggacaact ttcatccggc agcagagaag attgtgaaga acttctggcc
    24421 tggaccatta actttgatat ttaagatcaa aaaagacact ttgccctcag ttgtgacggg
    24481 aggattatcc acagctgcat ttcgtatacc ggataacaaa aaaacactag aagtaatcga
    24541 actttccggt gttccgcttg tcggtccaag tgcaaatacg tcgggaaaac caagtccaac
    24601 gactgcagat catgtatatc atgatctaca aggaaaaatt accgggatta ttgatgatgg
    24661 agctacgcga attggtgtag aatccactgt tcttgattta agtgatccaa cagccatgcc
    24721 tatgatttta cgaccaggtg cagtaacgaa agaacaaatc gaagcagtca tcgaatctcc
    24781 tgttgctatt gatcagcatc ttgtaaaaga aaatgaaaca ccaaaagccc ctgggatgaa
    24841 atacaaacac tactcacccg atacacgtgt tctgatggtc agagatggcg attggtcaac
    24901 tgcagttcaa tgggcaaaaa ataagaagat ccgtgcaggt gtcatagcaa gccccgaaat
    24961 tgcggatcaa gtgcggacag atactgcagc tgtttatatg tataacgaca actcagtaga
    25021 agcggcggct aaaggattgt ttgcaggtct aagaggatta gatgaaccaa cacttggatt
    25081 agatttgatt tttgtacaag tgtatccaga aactggattg gggaatgctt acatgaatcg
    25141 cttgaaaaaa gcagcaggac aaaattattt tgaaaaatga tcaattggtt attgtcgcat
    25201 tcttttagta tgatacaatg ctatcaagta aatcttctat cagtgaacga aaaactaagc
    25261 gtaactaatc gtaatagaag agatacgaat atttggcaat ttgtttttga tgggaaaaat
    25321 aaaattgtag gaaaataaaa aggagctgtt gtttgtggta gattacaaaa cgtttgaccc
    25381 tgatctttgg gctgcgatcg ccaaagaaga agaaagacaa gaacataatt tggagctgat
    25441 tgcctctgaa aactttgtct ccgaagcagt gatggctgca caaggaagta tcttgacaaa
    25501 taaatacgca gaaggttatc cagggcatcg ctattatggt gggtgtgaat ttgtcgatat
    25561 cgttgaaaat ttagcgattg accgtgcaaa agaattattc ggagcgaaat ttgcgaacgt
    25621 ccagcctcac tccggctctc aagccaacac ggctgcttat ctcgctttgg tagaaccagg
    25681 ggacacgatc ttaggaatgg atctttccgc aggcggacat ctaacacatg gatcaccagt
    25741 caacttcagt ggcaaaacct atcactttgt tgcttacggt gttgatccga caacagaagt
    25801 tattgattat aatgtggtac gtattttagc tagaaaacat caaccaaaat tgatcgtagc
    25861 aggtgccagt gcttacggac gaacgatcga ctttgcgaaa ttcagagaaa tcgcagatga
    25921 agtaggcgca aaactgatgg tagatatggc acatatcgca ggattagtag cagcaggact
    25981 tcatcctaat ccagttcctt atgcagatat cacaacgact accactcata aaacattgcg
    26041 tggccctcga ggcggcatga ttttgacaaa cgatgaagca ttagcaaaaa aaataaatag
    26101 tgcggtattt cctggtattc aaggcggccc tctagagcat gtgattgcgg gtaaagcagt
    26161 agcattcaaa gaagcattag atcctgcatt taaagaatat agtgaacaaa tcatcgccaa
    26221 tgcaaaagca atggtcaaag ttttcaatca agcaattgga accagagtca tttcaggggc
    26281 gacagataat catctgatgt tgatcgatgt tcgcgagtta ggtataaatg gaaaagaagc
    26341 agagagcatt ttggacagtg tgaatatcac tgtaaataaa aactcgattc cttttgaaac
    26401 actaagtcca ttcaaaacaa gcggtattcg aatcggcacg ccagcaatca ctactcgcgg
    26461 gttcaaagaa gaagatgctg taaaagtggc tgaactagtt gtcaaagcat tacaagcaaa
    26521 agatgataac gctcaactag acgaagtcaa aacaggtgta cgcgaattaa ctgaaaaatt
    26581 tccgttgcat aaaaaataaa aatttaaatt ccattagtaa acagaaaagt tcaatgttgg
    26641 gcttttctgt ttattttttg ggaaaacgga ttctttgaca atatcggtga tgctctctag
    26701 tatatgaggt gtaattttag tcttcacact aaatctcagg gaggaaaagt catgactgat
    26761 cgtatctcgt tagaaaagga agcagtagca ttcagtgatg caaatgcgcc tcatccgcgt
    26821 atctatgaac tagcaccgga agaaggaaga gttttactag aagaagtaca agaatcacct
    26881 gtgaacaaat attccgtcga tattgaagac ttgatggtgg atactggcga atggggaaaa
    26941 ctcaatattc gtctcgtacg accagaaggg aatatggata aattaccagt tattttttat
    27001 attcatggag caggctgggt ttttggcagt gctcaaaccc atgataaatt gatcagagaa
    27061 cttgcggttc gtacgaattc cgtcgttgtc tttccagaat atagtcgatc accagaagca
    27121 aaatatccaa cagcaattga acaaaattat gctgtgttgc aacagctgaa taaattagcc
    27181 aagtcaaaaa atctggatct cgagaatcta atcgttgcag gagattcagt aggtggtaac
    27241 atggctacag tcatgacgat cttaacaaaa gaacgaaagg gcctcccaat atccaagcaa
    27301 ttgctgtatt atccagtcac aaacgctgat tttgatacag attcctatca tgaattcgga
    27361 gaaaactatt ttttgacaag agaaggaatg atgtggtttt gggaccaata tacgacgaat
    27421 gaagaagagc gtgctgaaat cacagcttcg cctttgagag caaatataaa tgaactaaat
    27481 gatcttcctt cagcaatgat tttaacggga gaagcggatg tattaagaga tgaaggtgag
    27541 gcttatgcgc gcaaacttcg tgatgcaggg gttcctgtta cacaagtacg cttccaagga
    27601 atcatccatg attttgtcat ggtaaatgct atggatcaga caaaggcaac gcgtgcagca
    27661 atggcattat ctacttcttg gatcaagaaa aaagaataag ctttgcttgt ccgcaggaaa
    27721 acttcttttc aaaaacggat tttcttagga aaaatggcga gtttgactag tcaatcactt
    27781 gattttgttt tacaattaaa aagaaacgac agaacaaagg agagcttaaa aaatgggcaa
    27841 atttcaagtg atcgatcatc cattgattca acacaaacta actattattc gggacaaaaa
    27901 ctgcggaaca aaggtttttc gtgaagtagt cgatgaaatc gctatgttga tggcatatga
    27961 agtatcacgg gatatgccat tggaagacat cgtgatcgaa acaccgatca ctgaaacaac
    28021 acaaaaaacg ttgtctggta aaaaagtagc gattattcca atcttgcgcg caggtattgg
    28081 aatggttgac ggtatcttgg aattgattcc agctgctaaa gtgggacata tcggtcttta
    28141 tcgagatgaa gaaacattgg aaccacatga atatttcgtc aaacttccag aagacattga
    28201 tgcacgtcag ttgtttgtag tagatccaat gttggcaaca ggcggatcag caatcatggc
    28261 aatcgatgca ttgaaagaac gtggcgcgtc gaacattaaa tttgtttgtt tggtagcagc
    28321 acctgaggga gtaaaagctt tgcaagaagc gcatcctgat attgacatct atacagcttc
    28381 tctagatgaa aaattgaatg aacatggtta tatcgttcct ggactagggg atgcgggtga
    28441 ccgtttgttt ggtacaaaat aagtaaatac gaaaaagggt gtgacacaac ttttgtcaca
    28501 tccttttttg gtcattttta acgtttgatg cagaacagct ttttcacagc tttcgtttgc
    28561 atataaaaaa accatcccaa aaatgcagag atggtcaaat tcaaatcttt tttagttctt
    28621 tgtagttaac gattactaca cccttacttt gcgtcatcaa gttcgcattt tctgcagttt
    28681 catcaatctc aacaacagcg gaatttttca attgctttga aatcgttcca gtttgcgact
    28741 tgccgtgaat tgtgaacatt acgtgagtat tgatcttgaa tttttcacca atttccgatg
    28801 gtacgatagt tgtatcaaat tttccaaaag ttgccatttt cataaaacct gcctttcaca
    28861 tcaattatac cacgttttat gaaaaatatc atgaaattaa actaattttc catcggactc
    28921 tttacaatca gccaaataga gtatataatt tcttttggat aaggccagct tagctcagtt
    28981 ggtagagcaa cgcactcgta acgcgtaggt cgtaggttcg atccctatag ctggcattaa
    29041 aagagaagac agatatatca aggattttta atccattgat acatctgtct ttttgtttaa
    29101 aggaaataaa gtatgctttc ttagcaatag agcatgtatt atttctataa aaatttagga
    29161 gaataaaacg actagcaata tagctgtgaa atcaaatatc acatgaactg cccaacttgt
    29221 aatgatcgag ttgcttttaa tggcagcttg gttgaataac aatcttgcca ccccttgtat
    29281 caaaaggatg tgtgctacag tgtgtaaaat atttccatta tcataagtac tgtaatggat
    29341 aaatccaaag atgattgctg acaagaaact agcgacagga atgctgaact tactagaaaa
    29401 aatagcgaga aaatacactg agaacaattc ttctccaagt atcatgatag gcaaaataat
    29461 aaataataac gggctgaaag cctcatttat tggattgcct gttaaacccc atttcaaaat
    29521 ttgttgcaaa aataccgaga ctgcggcact gattactaca ttgataaaaa aatatttggc
    29581 tatatttttg acaggagcga caggtttcga gaacattttt ttcgtatctt ttttaccaaa
    29641 aacaagtgta aatgctataa atccagtgat agttaaagaa aaaatgctaa taatggatgg
    29701 cgcaaatagc aaccctaaca aaccgagtac aacgacaata gtgcccaata tccctttttt
    29761 aagttctatt ttttttccta aaatgttatt catgataata actcctacta ttcagttttt
    29821 gaagatagag tcagtataca tgaagagtta aaataggcaa tgttttttta tggatatttg
    29881 tattgctaaa aatttatctt atatattcat tttgattata ctacttttat ttgtaaaaat
    29941 tttctagcaa aaccaagtgc ttgcaactga ttaagcgtac aattttttag atgttccgag
    30001 cgtacttcaa tcatttcaaa ggagttgcta cttaaatcga tattttttaa atcagtatca
    30061 aaaaagctag cattttctaa atcattagta gaaaacatta ttttagtgag ggaacagtct
    30121 acaaaatctg cgcttgataa gacagagtgc tcaaaggtac tttcttttat gtttgaacca
    30181 gataaattta catagtccat tttacaattc tcgaataacg tgttcccaat ataactttcc
    30241 atcaatttag tacctgttaa tttacaattg acaaatttca cgcgatgcaa catgcttttt
    30301 gtcaatatac agcctgaaaa atcacagttt tcgaatatac agtcggtaat ctccattctt
    30361 tgaaaatcat tggaagcaaa aaatgcatac ttgaaaatag atttgtaaat aattggatgg
    30421 cagattattt ggttttcgat gacctctttt tgtgaaagga tattatacag ttcaaaatct
    30481 tcgttttcat agatatgaaa aaaggatcct ttattcaagc ttgcttcatt aactttagga
    30541 aaaatcatat ttaattacct ccattttcat aaagtcatat taacaaaagc taaaccaact
    30601 tacattagca gaaaatgaga taggttttcg cctttttcga taacgatagt gtcgctatct
    30661 cttgaaacgt tcttagaatt gccctgagag acacaaatta ggcgtaattc taataaaaat
    30721 cttagaataa aaatttttac tgatttaatt tagaatgttt ttaaaataaa aatcgttgcc
    30781 ataacagtgt attgaaaggt tttaacaatc cagtaatgat ttaaaaaaat ctagatgtag
    30841 cgtatacttt gtttatagaa tgtagtggga agaaatatat ttttgggagg aaatcgtgat
    30901 gtctaaaagt gaagaaatag aaaacatggt actaaaatta tatgatctcg ttttaaatcc
    30961 agagatcaaa gaaaaggaac gtatactttt gatagacgca aagacaggct tagaaaaagg
    31021 tcagtattat cctaaagtca taaataattt ggaacgtagt ttacgtccgt tagcaatcag
    31081 gggagaattg tctaaaccag tatcaccgtt ttatatggag atatcaacaa taggaaaatt
    31141 tgaaaaagaa ttaggtcgag gaatggcttc ggcaccgatt acttttgggc atttataaaa
    31201 acgagttatt catctcaatg aattaaaaag tatagtcaat ctcataatgc aggctaggtt
    31261 catttatttg aacagagtct gtattttttt attctaaata aagaaacaga tgtttcttta
    31321 tattgtattt tatcctttaa ttaacacaaa aacagctagt ataaactaat aatgttaatt
    31381 aaaagtgtat tttatgaaac tatcgttatt tattaatgtt gcattt
//
LOCUS       DACDYJ010000029        30326 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDYJ010000029 DACDYJ010000000
VERSION     DACDYJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.029"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..220)
                     /locus_tag="IWM51_001348"
     CDS             complement(<1..220)
                     /locus_tag="IWM51_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9970775.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGN"
     gene            complement(217..510)
                     /locus_tag="IWM51_001349"
     CDS             complement(217..510)
                     /locus_tag="IWM51_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970776.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            complement(547..684)
                     /locus_tag="IWM51_001350"
     CDS             complement(547..684)
                     /locus_tag="IWM51_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9970777.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(686..1117)
                     /locus_tag="IWM51_001351"
     CDS             complement(686..1117)
                     /locus_tag="IWM51_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970778.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            complement(1131..1625)
                     /locus_tag="IWM51_001352"
     CDS             complement(1131..1625)
                     /locus_tag="IWM51_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970779.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     ESVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            complement(1625..2074)
                     /locus_tag="IWM51_001353"
     CDS             complement(1625..2074)
                     /locus_tag="IWM51_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970780.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(2078..2695)
                     /locus_tag="IWM51_001354"
     CDS             complement(2078..2695)
                     /locus_tag="IWM51_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970781.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(2695..3603)
                     /locus_tag="IWM51_001355"
     CDS             complement(2695..3603)
                     /locus_tag="IWM51_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP9970782.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(3616..6375)
                     /locus_tag="IWM51_001356"
     CDS             complement(3616..6375)
                     /locus_tag="IWM51_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP9970783.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            complement(6372..7076)
                     /locus_tag="IWM51_001357"
     CDS             complement(6372..7076)
                     /locus_tag="IWM51_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9970784.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(7088..9397)
                     /locus_tag="IWM51_001358"
     CDS             complement(7088..9397)
                     /locus_tag="IWM51_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP9970785.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGGVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(9592..10056)
                     /locus_tag="IWM51_001359"
     CDS             complement(9592..10056)
                     /locus_tag="IWM51_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970786.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(10056..10682)
                     /locus_tag="IWM51_001360"
     CDS             complement(10056..10682)
                     /locus_tag="IWM51_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9970787.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(10689..11054)
                     /locus_tag="IWM51_001361"
     CDS             complement(10689..11054)
                     /locus_tag="IWM51_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP9970788.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(11044..11382)
                     /locus_tag="IWM51_001362"
     CDS             complement(11044..11382)
                     /locus_tag="IWM51_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970789.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(11372..11698)
                     /locus_tag="IWM51_001363"
     CDS             complement(11372..11698)
                     /locus_tag="IWM51_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9970790.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(11679..11957)
                     /locus_tag="IWM51_001364"
     CDS             complement(11679..11957)
                     /locus_tag="IWM51_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP9970791.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(11959..13323)
                     /locus_tag="IWM51_001365"
     CDS             complement(11959..13323)
                     /locus_tag="IWM51_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9970792.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(13336..13911)
                     /locus_tag="IWM51_001366"
     CDS             complement(13336..13911)
                     /locus_tag="IWM51_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAP9970793.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWD
                     DLSEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(13877..15064)
                     /locus_tag="IWM51_001367"
     CDS             complement(13877..15064)
                     /locus_tag="IWM51_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9970794.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(15128..16855)
                     /locus_tag="IWM51_001368"
     CDS             complement(15128..16855)
                     /locus_tag="IWM51_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9970795.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(16852..17304)
                     /locus_tag="IWM51_001369"
     CDS             complement(16852..17304)
                     /locus_tag="IWM51_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAP9970796.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(17416..17796)
                     /locus_tag="IWM51_001370"
     CDS             complement(17416..17796)
                     /locus_tag="IWM51_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9970797.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(17793..18179)
                     /locus_tag="IWM51_001371"
     CDS             complement(17793..18179)
                     /locus_tag="IWM51_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970798.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(18181..18459)
                     /locus_tag="IWM51_001372"
     CDS             complement(18181..18459)
                     /locus_tag="IWM51_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970799.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            18507..19235
                     /locus_tag="IWM51_001373"
     CDS             18507..19235
                     /locus_tag="IWM51_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAP9970800.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(19381..19857)
                     /locus_tag="IWM51_001374"
     CDS             complement(19381..19857)
                     /locus_tag="IWM51_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HAP9970801.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(19817..20164)
                     /locus_tag="IWM51_001375"
     CDS             complement(19817..20164)
                     /locus_tag="IWM51_001375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970802.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(20151..20336)
                     /locus_tag="IWM51_001376"
     CDS             complement(20151..20336)
                     /locus_tag="IWM51_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970803.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(20337..20609)
                     /locus_tag="IWM51_001377"
     CDS             complement(20337..20609)
                     /locus_tag="IWM51_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HAP9970804.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(20606..20809)
                     /locus_tag="IWM51_001378"
     CDS             complement(20606..20809)
                     /locus_tag="IWM51_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970805.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            20934..21146
                     /locus_tag="IWM51_001379"
     CDS             20934..21146
                     /locus_tag="IWM51_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970806.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(21179..21373)
                     /locus_tag="IWM51_001380"
     CDS             complement(21179..21373)
                     /locus_tag="IWM51_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970807.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(21381..21569)
                     /locus_tag="IWM51_001381"
     CDS             complement(21381..21569)
                     /locus_tag="IWM51_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970808.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            complement(22196..22561)
                     /locus_tag="IWM51_001382"
     CDS             complement(22196..22561)
                     /locus_tag="IWM51_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970809.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(22601..22903)
                     /locus_tag="IWM51_001383"
     CDS             complement(22601..22903)
                     /locus_tag="IWM51_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970810.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(22903..23202)
                     /locus_tag="IWM51_001384"
     CDS             complement(22903..23202)
                     /locus_tag="IWM51_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970811.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(23365..23634)
                     /locus_tag="IWM51_001385"
     CDS             complement(23365..23634)
                     /locus_tag="IWM51_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970812.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(23646..24395)
                     /locus_tag="IWM51_001386"
     CDS             complement(23646..24395)
                     /locus_tag="IWM51_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9970813.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(24398..25084)
                     /locus_tag="IWM51_001387"
     CDS             complement(24398..25084)
                     /locus_tag="IWM51_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970814.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            complement(25090..25761)
                     /locus_tag="IWM51_001388"
     CDS             complement(25090..25761)
                     /locus_tag="IWM51_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9970815.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(25754..26095)
                     /locus_tag="IWM51_001389"
     CDS             complement(25754..26095)
                     /locus_tag="IWM51_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970816.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(26273..26971)
                     /locus_tag="IWM51_001390"
     CDS             complement(26273..26971)
                     /locus_tag="IWM51_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9970817.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            27058..27528
                     /locus_tag="IWM51_001391"
     CDS             27058..27528
                     /locus_tag="IWM51_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970818.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(27571..27750)
                     /locus_tag="IWM51_001392"
     CDS             complement(27571..27750)
                     /locus_tag="IWM51_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970819.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(27737..28075)
                     /locus_tag="IWM51_001393"
     CDS             complement(27737..28075)
                     /locus_tag="IWM51_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP9970820.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(28091..28294)
                     /locus_tag="IWM51_001394"
     CDS             complement(28091..28294)
                     /locus_tag="IWM51_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9970821.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(28309..29085)
                     /locus_tag="IWM51_001395"
     CDS             complement(28309..29085)
                     /locus_tag="IWM51_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP9970822.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            29384..29704
                     /locus_tag="IWM51_001396"
     CDS             29384..29704
                     /locus_tag="IWM51_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9970823.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            29722..30153
                     /locus_tag="IWM51_001397"
     CDS             29722..30153
                     /locus_tag="IWM51_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9970824.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT         8511 a         6333 c         4659 g        10823 t
ORIGIN      
        1 tgtttcctcc ttcttcgctt tcagccgaga acattttgta ggttcgattt gatacaccca
       61 acacactccc taaaaacgcg ccaaaaccag taatgatgac aacacagata tctgtgtact
      121 gccaattgag cgctttacca actaacccca cgaaagtagc tagtgcggga ataattacca
      181 gtgcgaacca ttttagtact tcgaacgttt tattattcat tttcttctct ccctaaacaa
      241 agttttgatt tgttgcgtgt gttccaccaa tttttctgta tgtgtatcta atctttcatc
      301 gtgtttcttt agttcttcat gaatcatcaa tcgatctgat ttgctcgatt ctaaatcttt
      361 agtcagcaaa tctaaattgt ggcttacttt tgaaagagtc tcagtaattt tcgagaaaga
      421 tgcagcaatc ggtctaatta ctaataaaat caaagaaacg atagcggtta ttgatcctgc
      481 tatcattccc cattccccta aattaatcat gtgacaactc ctttacctta aataaaaacg
      541 catcaattaa gatgcgctct tatctttatt aatgatttta tctgcttctt cgtctgtaat
      601 gcataatgga acgaaaacca ttacttgttc gttagtgaaa cagccccagt catacattag
      661 tttgatatct tcatatgaat acatgttact tcccaccttg taattgtttt ttgatttctt
      721 cgatttcctt ggtattttgt acagaagcaa gcatcgtttt tgaattcaac tgcgctaaac
      781 tttctgactt ttctttcaat aagacatttt cttgcttgat tgctacattg tttagcattg
      841 ctttagaatt tagctgtttc aattcatcat tttctgcctt cagcgtttcg tataatgcct
      901 taatattagc caattcatct acgctatcaa cagcattccc ttttttctct tcttcagtag
      961 ctaactcaac ccactgtttt ttatcaaaat caaatcttgg tctccagtta ggaactggtg
     1021 gtttaatctc tgtacagttt tcagggatat tttcttgatt gttcaaaatg atttgctcga
     1081 atccgtaagg cttgattgat ttgtaaacta ctttcattag atttcctccc ttaaattgaa
     1141 taagtgataa caaatgaata agctgatcca taacttgaat ttcttctcca tttaatggct
     1201 ccgtctgcgc caatagatag ttgagcactg ttcaaagtag aacggtctat tgacccaacc
     1261 agttgttcaa aactaattgg tgtccgatag ccttctggaa cagttaaaat aaccgactca
     1321 ttcccgccac tactttttcc gtttaaagcc acaaaatata tagaaactgt ttttccttca
     1381 cgataaagct ttgctgtccc tgtattaccg ttagtgacaa ccagatttac cacttcatat
     1441 ctgttatcat cagtcgtcaa aacacgttta ttattgattg ttacagaacc tgaaaaattc
     1501 ttatgtcctg caatggcttg gtctccatct gtctgcacca atgcttcttt accatcaata
     1561 gcttctacat gcgtttttaa gtatttagca actccgtctt cttttaattg aacaatatca
     1621 gccattaaac cgtccccact ttctcaaatg taatattcgc taatccatca agtttcactt
     1681 tatcggttgc tgacattaaa cctgctgtcg tagttgtagc attacctgga tttttctgtg
     1741 ctccagctgc aattccatcc aacttagttt tatctgtgga cgacatcaac ccatttgctg
     1801 tagttgtagc tacagctgta gttgtggcat ttattccagg atcaccttta tctccctttg
     1861 gtaaaacaaa attaaatcta gctgcagatg atgttcctac attcgtaaca gaagcggttg
     1921 aaccactaga aacggttcct atggtaattg ttgctgcttg gccaggatcg cctttatctc
     1981 ccttcaccgt tgttggtttg ccttctatag cattccaatg agtttgtgga taaacctgta
     2041 cattgctttg ttttattttt acgatatctg tcattctcta tacctcccca attttttcaa
     2101 aagtaaaatt tggaattctt tcgtttgtgt aattttctgc ttgatttaca gcttcttgga
     2161 atttttgatc tacatatgac tgattaacac caccagtccc actaccacct gtagaactaa
     2221 ttgttccatc ttctgcaatt gatatatttg cacctgcttt taatattttt agagattcta
     2281 attttccttt taattcttca gagaaattga agtctgtttg cttagttgca gataaaacac
     2341 cttcttcagt aacttctaag agttccccaa cttttatacc tcctaattgt tcaggagtag
     2401 caatcggcaa aatatatgtt ccaccttctc catttacgat cttttgaaac atttcagcag
     2461 taagaatacc atcgcttgtc tcgcttgcat aaggtagttc agtaattgca ttctctaatc
     2521 ctaaatcagc tttcgttaaa atgactgcac catatttacc attaaccgaa agaacttttg
     2581 attgtcctga tatcattttt tctaagccta aaactgcaga gacatgtgta attggcataa
     2641 attgacgttt aacaccagat tcatcagttt ccatcattct ctttacttta accatcaaat
     2701 caccccaact ttctcaagag tgaaaacatt ctgttttgga tcatcaacag ttgcaataat
     2761 caaagctcct tcttcaattg gaaattctac tgtttcaatt ttttcgactt cgtgattatc
     2821 tgaaaagaga tcgtcttgaa gaatatcagt cacttcaatt tcaccgtatt caatcgtgaa
     2881 aagtgctgct ttcatttttt gatacaaata atttagatcg gataggaaac gttcagaaat
     2941 cgattcatgg cgtacacctt gaagatctac acgtgcatcc attaattcgg ctaacattac
     3001 tccacctgga tcgatagact tcaaaatatc cttgattgat tcaaaccatg acaagtaatc
     3061 tgattcttgt ccttctctcc aatcttggaa actattctcc tgttcctctc tccagcgatc
     3121 aaattcttct ttcctttcat tcatccagtc cgtaaaatcg cctttatttt cattaataaa
     3181 atctgtcata tcagcaatta aatcttcaat tgattgccaa taagaaccca tttctccttc
     3241 tgtttttgaa acagcattga tgacaaaata agagaagtct tgagttgttc caatcaagtc
     3301 ttctccttta aatataatga aattggctgt ttgtcgatgc aaacactgca tggaatattt
     3361 atcaaaaata tatttgattt tccctttttt agcatccaca atttttgttt ctaattggac
     3421 tggatatttt ccgccaacaa ctgattcaaa atatacctta cattctgata aatcatatgg
     3481 aagaccattt tcgacaattg tagcttccat aacttcagtg tttttattgc cttgtcgaac
     3541 ttgaatcatc cccacgtaat tatagggttc tgttgtactt aatatgacat tccactttgc
     3601 cattaaatca cctccctatt ttggtggtat gacaatggaa gaaattgcac ttgcactata
     3661 atactgtcga tctaactttc cacaaatcat acctaactct gtgttttgtt cataagtttg
     3721 catacgacca ttagctaagc ctcggataac gccagtatgt ccataagttc catctgcaaa
     3781 ccatgaaccc acttgtccac ctcttgccca attaataatt gcaccaacta ctaattgatc
     3841 atatctaggg ttttgaatca ctttccaacc aacagcagac caatcatagg caataccaat
     3901 atcagatgct gcagaagtat ttcctatcac atgagttaaa ccatattttg ttccagcacc
     3961 catgccacaa ccacccagat atcctgaata ttctgcagac aagccataac actgaccatt
     4021 gccaattcgt tgtccaatca aagactctaa atgttttagt cctgcttctc cagtagctcc
     4081 tcctggtttt aaatctttaa atttgttata ccagttaact gcataatctt gacgttcagg
     4141 atgtgtcgct gccggacgtt catagtttcg ttcaaaagca tatgctgctt gtcttggatc
     4201 agtacaggcc ttaaatccat caaccgtcgt aggttgtact acgcccatcc attgtccatt
     4261 tgtaaatgtc caaataagca atcgaacttg tgcatctaag ctcgttattg gttctttaat
     4321 acctgcagca ttaaataagt tttgaacata aacttttcca tcccatgttg ctggaccaac
     4381 aagaggatat gaagaaccat cccactgaac taatccgtat gctggtccac ctatttgaac
     4441 agtatcagga tcaaaagttc ctcctgtttc ttgttgaatg ttccccaata ttccacatgc
     4501 agattgtttc gtaaatccgt tattacacaa aatatcgtat attctccaag ctctcttttc
     4561 tgcatctgtt tttaattcat caggataccc accaggtgtt tctcctccgc cagatggacc
     4621 gccattttgt cctggtatca cttctttacc tctaacagta agttttccct gaatatctaa
     4681 atcaccgaaa taatatgctt ttccgtttcc taataacaca aatccttttt ctgcagttgg
     4741 agaaattaaa atgtattttc catcgccatt tgtacgtatt accaaagaat tttcctcaag
     4801 aggtgtaggt gttacagcat ttggaaatgg atttcctgct gaatctgttg ttccgatcgt
     4861 tccaatggat tcttttctat tccagaattc catacctttt ttggttagct ccatgatttt
     4921 tttgttttca ttccaaattt gaagcgtgcc ttttacaagt tttaacacat caccataagc
     4981 attaaaggaa gtttcgaata cttctgcatt tattgttcct actttgatga agtcagcaac
     5041 aatttctcct tttgatgtga tagcaatacc gaacgggcca tttactccgt tgtctgaata
     5101 acctaagcca tttaaattcc aacgccacac tcgctttgca ttcgccacat ttggagtatc
     5161 catgataaga atctctgatg gagctttttc tggacgaaaa acgacatgcc caccagaatt
     5221 accagtgatc catgccgtcg cattcaacac attttgaact aacgtttctg ttcgattgtc
     5281 gattttcttt ttcaattctt gtgcttgagt atttactgcc gaagtgtaca gtgataaatc
     5341 attacctaaa acaatatcct tatatttccc aagcgttggg aaataagtgt attcgaccat
     5401 acgctctttt atctcgatat ctagctcttt cgccctaaca tgagcgacat caccaaagtg
     5461 taaagacgct agctcttgat acatgtcacc gtattcgagt gtgtgttcca acgccaccat
     5521 actgactgta tgagtagctt ttggctcatg gatacgatca ttgtcaaata aggttttgcc
     5581 ccatttgatc aattcatcaa ctgttttaca atctccgttt tcacgtttgg caatacgtcg
     5641 attctcgttg gttactccat caatttctaa ataaccatat tcgataggtt ctttgtcttc
     5701 atcataatcg ttatcaggaa caccgccaac caaatacaaa ctattgacaa ttgattcttc
     5761 atcaatttct tcttcgattg cttctaaatt gatgccaaaa tcaatccgaa aaccattatc
     5821 tgatccaatc tgttttacca gtttcaaatc atagttatcc atatcaagtt ccgcgctagt
     5881 cacgccagtc aaattttgat taccgttatt tgaaccaatg attgcttcaa ctggcgcgac
     5941 ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa agatagttaa atttttgatc
     6001 aaatgccaaa ctgtttttaa gattggtcat gatctgagac ccattgccgt tatccgtaaa
     6061 cgacttgaca ataaagttct tattggccat aaaaccaata tgccgagctg ttacagaaac
     6121 ggaggtcaaa ttctttttga cattataaat ttcaaaatat tgccatgatc catctggtac
     6181 cttcgctttt ataaaatttc ctttttttaa ataagagcga tactgtccgc ttcttgaata
     6241 gttaccataa aaacgatatt ggccattcag cacacgattg atttctggca aatcttccca
     6301 atcaactaac gacgctccgt tcacgctcaa atcatctggc atttttttat aagcataaat
     6361 aaattcttgc gtcacagata cacactccta ttccaaaacc gcacttcttt aaattttccg
     6421 gatattttta cctgattcca ttcaggctgc aatactggcc aatcaccacg cgtaaataaa
     6481 ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa ccacgattgt tgcagctaac
     6541 gtattaagta ttgttaggct tttatcaccg acagcgatcg ttatatctcc tccgttcgat
     6601 tctatttcca gatagggatg tgcaatttcg tcaccatgat caaaaatatc cataacacta
     6661 gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga gtggctgaca acgaaaagta
     6721 acatcgaatg tataaaagaa gccccactcg ttttcgaaag ggacttcttt atccatgctg
     6781 cagatagcct ctagatactt gtctggatca ttatgtgtga ttaattggct tttaccagtg
     6841 agccatcgtt tgacttctct cagtttcgaa tgtggaatag tgatcccttc tatttccaaa
     6901 tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc cgcttcggcc ctgaatcgtg
     6961 taagtttcgt atcgcttgtt cggcataata tctggaagct ctgcctcaat gatgcaattc
     7021 atatcaatta ctgcattccg atttttccaa ataaaatttg gttcatctgg attcataaat
     7081 tgtcgactca aataggaaca cctcccaaat cacgaatagc ctgtttgttt gctttagcaa
     7141 atttacgatt catacgatct aactcagatg gattgtttgc atcgactttg ccaatgtgta
     7201 tatgctgttc aatgctacct ccagaaactc ttccactgat ccctttgcgt ttttcttcgt
     7261 ctgataatgg agtaactgtg gttttaccat tcttggcagt cagtaactca ggtccagctt
     7321 caccaacaat cgcttgtcca ttgattagat gaccgccttg tgctaaataa ggcagttttg
     7381 aaatagaaaa tgattttcca cctacaccag gaacccattt tggtatcttt atattattca
     7441 atccaccaat aaatccattg atcaatccaa tcatggcatt aataggagct ttccctacag
     7501 caacaatacc atcgaaaata cctccaaaaa tatcaactag accttgccat gcttttgacc
     7561 aattccctgt aaatactcca gtgataaaat ctagaaaacc actgaaaatt cgttttccag
     7621 cattataaaa attattgaaa tttgctataa caccatcgaa aaatccacca aaataaccac
     7681 ttaaaaaatt aaatatttca acagctacat ctgaaacacc tttgaagaaa gaattcactc
     7741 catcatgaaa ccatttaacg ttattatatg cccaaattag tccagtaact aatgcgccaa
     7801 tagctataac tgctaatgca aaccagccac cagacattcc aaataaacca gcaagtcctt
     7861 gccaaactcc tataaaactt ttaactcccc caacgatttt tgttacagat cccatcagcg
     7921 aaccaagaac gattagtact ggtccaactg ctgccgctat accagcaatt gttatgatcc
     7981 aattctttgt atcttgatct agagaccccc accatttaga gaattcctgt aataaggaag
     8041 ttgctttttc aaaaaaaggc aaaaggctta cttggactgc ttcacctacg tcagccattg
     8101 ctaattttgc attgttcata gcttgatcgg cttgatcaat tgggtcgaga gtagcatcga
     8161 atgtgtctcc tacagctccc ccactttctc cagcagtttt tgctaaatct tccaggttca
     8221 aagtacctct acgaattgca tcggccattc taggaccgcc tttagtcccg aatacttctg
     8281 ctgctgcatt gatggcttct gtttcagaac ctgcattttt taccttgtct tgtagttctt
     8341 ctagtccttg acttaaagat tttccgtctt tcgcataagc cactgttgct ttagataaac
     8401 tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa ttgtccaagt aaagtgaccc
     8461 cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc gccatcaatt gctttttgca
     8521 tcaagtcgtc tacagattga ccagtattct gcgatgtttt cgttgttaca tcaaggacag
     8581 aattcaaatc atcatattct agtccataag catcaatagc ttgtcgagca gatattgctg
     8641 attgcgaaac atctgtatca ttgatttcag cgtactgtag gagataattg gttgaatctt
     8701 ccagtttttt atccatgaat ccaaattgtg tatttacctc accaatagct tccccaaccg
     8761 tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa agactgtgaa agtctatcag
     8821 cttgatcacc tgttgctccg gtttttgtaa tgatggtatc aagtgcctcg tcaacttcac
     8881 caaatgctgc aagtcctgct gctcctgccg ctacaatagg cgcggttact ccaacagaca
     8941 ttttttcacc aacgcctttt actttctcac ctgtttcttc gattttctgg agtttttttg
     9001 ctgtatcaac agaaacgtca ccttgttctt taagtgcatc gttcgtctgt tctaatgctg
     9061 tacgaagttt attttctccg gtttcagaat tgagtaattg tttataaagt ttttctgatt
     9121 cagcagaata ttcaccggtt tctttgacag atttttcata ttcctctctc agcatttgag
     9181 atcgttgttc agccaatcct aattgttttt ctaatttttg tttagccgct cgcaattttt
     9241 ctgtttgagt tgcatccttg tccatcgcag acacttggtt tttgtactcg gcagcggcta
     9301 agttcatttc tttgttgatt tctttgattg ttttcgaata actgacttcg ccattcgttt
     9361 taaaattaag aacgacatca gattctttac cagccattta tcttagcgct cctttctacc
     9421 accaaggcga tttatccata gttacagatt gaggtggttc aaaatcggtg ttttgttgta
     9481 accactgtaa ataagatttg agccacaagt taggtgtaga ctttaaaaag aaactctcac
     9541 tccaattcaa tagagtgaga gcaacgtaaa tataaaaact ccaaggagtt cctatctctt
     9601 ccgattcttt ttgtttttct ttctttttgc ttgcgctttt tgaaagtctt gtggcttctt
     9661 ggattttttt aggtcttcca cctgaaatgt ctggctagca aaaatttcca tacaggtact
     9721 ataagcagac aacacctctc cactcattcc caaaaatttg aaaatagttt ctggatcttc
     9781 ctctaaacca ccagtacgca gcatggcata aatcaaggca cgcatgattt ttagatcact
     9841 aggcgataag ttagcagaag aaatttgtcc ttctttttta gatagcatgg cgttcatatc
     9901 agattcaaat tgcgaataat ctccaccata cacatctgct ataaattcca tcgtttgcat
     9961 agtaaatgaa atagggaatt ctgcgccttg aataattaca acagcagaat tttttaagtc
    10021 ttcaacgtga attccataat cagataaccg agccattaac ccgcacctcc aactttagcc
    10081 aatgttttcc actgttcttc atcgtataca ggttgagcaa tgaatttatt aaataattct
    10141 agagaagctc cgtctctatt tgaatcaaaa cttgcataca tcacattatt gtatttcagt
    10201 ccgatggaaa ccagatttgc agttacatcg tcaatttttg tttcttcctc tgcagtggca
    10261 tactcttcgt caatcacatt ggatagttgt gtattaggat accaaactgc ttttttaccg
    10321 ccattttcaa tatttccaat aaatccaaag gcgaagtacg gaaattctcg tgcggtattt
    10381 ttgctaaacg ttactccgct tttagcaact aatcctttga tttcatccat cacttctatc
    10441 ggaataccca cgtgatccaa cgcaagttca tgttttgttt ctcgcgacac acgacgaaac
    10501 attttgctgg atgcccattt ttctaatgct gttccattcc ctttgacacc gatttttgtg
    10561 gcaattggca atcgtaaaac ttcactataa gtcggtgcag ttccaacaga atctggcgtt
    10621 gccatcatcg caattaaaat gtcgtccaat ccttcaaaat aaaaagtatc ttgttttccc
    10681 aattaaaatc acccttccca taaatcaagt atttttctag tcataatttc ttcaattttt
    10741 tctttgtttt gttcatacgt accacttgca aaatgttggg cacgttgatt tgttgtgcca
    10801 ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt gaactttcac ttgatcgttt
    10861 tcaactacta cttttatttg atcgcgcata tgtttctttt ttaagagcga cttaggaata
    10921 ttaggaagca actgctttag ataaaagtta gctgcttctt ctaaagattc caacgataat
    10981 tttttcggat caacttttga aaggtttcct aaataatccg atatttcaga aaaaccattc
    11041 ttattacttg gcattttcca cacacctcac atatgtgtaa tagttggtta cggtatcgtc
    11101 gttctcatca ccttgtatac ctgtaaaatc tgaatatgga atgccagcat tttgcagcgc
    11161 ttgttcaata acaactaaat cctgttctgt tccaagtgta aaaaaagaga cttggtaata
    11221 aggtaatttt ttatagactt taccggatgc catttttttg ctgttactca catttgagta
    11281 cacaatgtat ggatacatcg tccctaatct ggctttgtct ctgaacactg ctaactttgt
    11341 tgatttcagc gctgttttca attcatcaaa gctaatcgac ataagctaaa ctcaactcca
    11401 tttctcttgc atcgggattc gtataaatgc gagtaatgtt atacgttaca gaatcaattt
    11461 tgagcgcact taatttctct gtgatggatt tatcccatct gactttaatt cgtctgacaa
    11521 cgtctgtctt ggcttgctgt gataaatatt tttcttgaga agtcacaccg agttcttcat
    11581 agaatattag acgcttaaat tcgtaaattg tagttggacg atcgtttccg tctgttcctg
    11641 ttttgatgtc tagcaattcg gctttccatc tgagattatt agtctgtctc ttcggcattt
    11701 tgaatcgctc cttgcacgat aaatggcgtc atggcattca tagctttgtc gagttcatcc
    11761 tctgaaactc tatattcata ggcaatgccg gcaaccatca aaataagata ttcttgttgg
    11821 ccaccagttg ctgttttgac ataatctttt gccatattta aataaaaaga gagcaaagaa
    11881 tcatccatgc cctcttcaaa atgaatatgt gatttgaatt tttcctctaa agacaattct
    11941 ttagtttgct cttccatctt aaccaccaac tggttttgta atttcgtagc gatatactgc
    12001 cggttcaaat ggagaataaa ccaattgacc atctagcaag ttgtaaattt ggaatccaat
    12061 ttgattttta ccagagaatt tttcaacaag tttttgaatt tccaaggcac caataacttc
    12121 ttgaatttta aatgcagaaa aatcgccaaa atataaaact ggcgtgtctg gttcaccctt
    12181 tttatctgct gcatctgtcc aatccacagg atagccaact aattggtaac caattccacc
    12241 ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca tctgttttca ttttttcaat
    12301 ggcagtcaaa gcagctcgat taataataaa gcgtcccttt ttcatcactt ctgtcactgg
    12361 tgtattttta aattcgatta atgcatcata taatttttgc ccagcacctg aagcagttag
    12421 atctaaaggt tttttaaatg ctacagcctt tttggctaat gcaccaggat tttcatttcc
    12481 agcgtcatca ccattgaaca tataattgat ttctttacgc acataagctt ttttcaattc
    12541 ttccacaaca atatcttcaa ctggaacacc agacattttt aataattttt tagttactgt
    12601 tgccaaagca tcgaattcgg caggattaag caaaatttca tcaaactgaa tagctgtttc
    12661 agcaatatca gttgaacgct ctttcttgtt tacattcgca tctgctttct tcacaagaat
    12721 tggatatttg acatctcctg atgttcgcac cactgttccg tatttacgaa gtaaattttc
    12781 ttcttgagca taagtaataa cttcagatgc aattacttct gggacagtaa ctgaaccgtt
    12841 gccagcttca atccctaaag ctcgagcttc tgcttcagaa atatttccag ctacaaaatt
    12901 agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg gtcatttttg cacgtgcctc
    12961 caatccgttc ttaattgatc caagtaatcc atctcgttgc tgttgagtaa tcattccaga
    13021 acgattttct gaactatctt cactgtctga atcttcttct tgatcgttgt cttcaccaga
    13081 acggctttcg tctgaatccg tactattcga ttgatcatct gtattgtcgt ctgtttcatc
    13141 agttccagaa tctgcgccta attcgtcttt aattccgttc aattcatcaa taacactgtc
    13201 aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat tctcctgatt caactttttc
    13261 acgtaattca gtcaatcgtt gctcacgacg tgccttcatt tttttcaata attctttatc
    13321 catgtatttt tcctcctacg cttctagcgc ttgattaatt tttttgatta atttttttct
    13381 ttcttcgacg tttttttcta attcatcacg acttcttaat gcagcttctg tatcttcgta
    13441 tgctggcaaa ggcacaatag aaacctcgta taattccact tcatggattg ttcgaagcat
    13501 tggttcagaa ttgtagtccc acgtttcttc agtcggcaca aaaccaaaac tacattggtt
    13561 gatgtctcct cgttccatag atttaactaa gtcccttgct accgttgtgt ccggtaaatc
    13621 aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga agggttccgc ttttagtacg
    13681 tcctagcaca ttggaccaat cgtgattgaa caaacaacga acatccgaat tactaattgc
    13741 ctttgagaat gcaccaggaa caatcacttc gcttaagtca tcccataaaa gtgtttgact
    13801 gttgaataca gcagcgtagc cgctaatggt cctcgtatga gtttcttcat cggaacgtgt
    13861 tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc ttcatttcca tcacctcctt
    13921 tcaagtcttg atcattcgtt ggtaaggaat catctgttgc atttttctgg ccaatcctag
    13981 ataagtcatt tgaaatatag atagcttgtg tttctggagt attctgttta ggaaaaccaa
    14041 gcatttctgc cacgttatct ggacttgtaa tcccggtacg aacgatgttg tagccaatat
    14101 ttgtttttgt tgagtaagga acaaaatcca aaatattaat tttccattcc actctatagc
    14161 cagaattagg cataaaaaaa agagcggtgt aatgttcgct cttgtttttc aatattggtt
    14221 tgattgcttt gttgtgcaga tacatcatcg ctttttcaat atctgtcttc atcagcgatt
    14281 gatacgtatc aacattgatt cctagaaatt ttcctaagtc ttttttgtaa acacctaaat
    14341 aattcaaaat tgccgcgtca tcaacaggac tttttaatgt ctcgatggaa tatccttttc
    14401 ccaaaggaat catcttaaca gaatgatcac tctcattttg cgttccttcc agttgatcca
    14461 atatagcttt gacaattttt gtttgggcgc tattatttgg attgatgtgg gcgtctagtt
    14521 taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc cgtcaaaact ttttcagcgc
    14581 tcagaacgcc ttctagagtg tttcttgcaa gatcaattat tccagcacct tttaatgaat
    14641 cagttccgat gttcttaatg tgtcgaatca tttgaccagg tattttttga ccattcattt
    14701 caaattcttc ttgaagtcgt tcattgattt tagttgttac accgtacgcc aaatgaagct
    14761 gatccctatc cgttaatggg aatgtctcac cattgatcaa taaagtattt gtttccaatt
    14821 tggtaaattc gaatcctgtc aaataatcat tgggactctt caaaatattt agtaagtgat
    14881 gatctttgac ttcgttgccg tcgggaccaa taacaattgg tgtggccaat gccacctgat
    14941 tagatatgtc ttgaactaat tcataaacat cagaagattc catgatagat gaatccgtca
    15001 catatctttg accataacgt gtgtagtgtc caaaaatatc ctcgatatat ccacgttttt
    15061 ccataaaaga atagactgca ttcgataacc gatcacgtaa tttcaatttt tttcaccgcc
    15121 tttctattta tctatagatg gaacttaggt aatcatctaa ctcatctgaa tcaatatctg
    15181 tcatttgatt catcgtttcc ttatgaccac acaaaaacgc cacgaaccca tcaatctttt
    15241 tctttgattg acgtttactt ggcgcttttt gtccattgat gttagtaatt gctacaacgt
    15301 tcaaagtgca ataaaggaat aatggattat caaattgaat tcgtttctca taaaacaacc
    15361 gttcgacatc gtcaaaagga gcattcaata ctttaggata ttgagcaact tcaacgcatt
    15421 ccaatcctaa gttttctaat ttctcaacaa gtttgtcact catcgctgga tcataattca
    15481 cttgctgaat atcatataaa ttcatgcagt cctctatgta ctgcaaaatc tgatcttgat
    15541 caatcatttt tccgtcgcaa aattcaacga aaccttgttc agccaaatca ctgtaaggaa
    15601 cattatcttc tttttctcga aactctaaat cttcattcgg aataaaataa agttgcttta
    15661 ctttaagaac cgctttacca tcttcatccc atgttgggaa gtttaaagat acacaggtca
    15721 aatctcgtgt acgtgataaa tctaaaccga tataacatgg ttcgccactt aaatcaccaa
    15781 gttcttgtgt ggtaactaaa catggctcta cttgatcctg ttcaaagaaa ttatccgcgc
    15841 cgttcacaaa tacatccaag tgtttcgtta gaaattcagc tttggaatga gctgaccgtt
    15901 gcgctgtttt aaatgccgat tctaaagcag ataaatcgac tgatattccc cagttaggat
    15961 tgcacatttc ccagactttt ctatccgtcc aatcataatt tttatttggc tcatagatta
    16021 gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc ttttgcttcg cgatatacac
    16081 gcattccaac agacgacgaa cctttcccag cagttgagat attaaacatc aatggctgtg
    16141 gtaaagatat ctgagcagac ttaaaattgt catactgttc cattttctcc tgtttatgca
    16201 attcatcgtt caatacaaaa tatggattgg atccctcgat attatcaata ttcttggttt
    16261 gaacaataaa tttatttgag taagccatat cttcatgaat atagtcataa gtaatactag
    16321 aaacagttcc ctttggcccc ctaaatattt tagttccttc aagtaggatt ggattgttta
    16381 agattgtaac tgcaaaaggt ttggcagcat attgcgcttg agcaaaatca gatgcgcaag
    16441 catagcaatc aactgataac gccccttctc cgtacattgc atatcctaat gaaccgacag
    16501 caatcaatgt tttaccattc ttttttggaa tctggacata agcctcacga gtaacgcgta
    16561 caatttggcc tttttcattt tctttaaccc aaccataaat ccaagaataa ataaattttt
    16621 cccagggttc taaaagaaac ggttttccca ccatttctcc ttttgtgtga acaataaatg
    16681 attctaccca atccatcatt tcattcgcgc gatctacatc aaaccaaata tcttttcttt
    16741 ttttccactg ataccagcga tctattgcta aacgaacggt cttaggatac ttcttaggtt
    16801 tctttcttac ttcttttgca aataaatcag cataatttac accaggttca atcatttttc
    16861 agcacctgcc ttcttacgcc atttatttct gtgcgctgct agttcatcta ctggctttac
    16921 ttctgggcgt gtaatttctt catcttttct tgcggtcgat cctccagcaa ttagtctacc
    16981 agtttttgct ttgtttgtta atcctaataa atctagagct ttcatcttct tatctgccca
    17041 agtttctact tgttgtgcca atggatgttt tgattgatta gtagctcctg atttattcgt
    17101 gaatttttgc gtctccggaa atcctttttc cttccacaat aaatatttgt gttggtaaat
    17161 ttcaaaaata tccaagtatg attcgattaa tggatcaaga gtgatggtgt acaaatcaga
    17221 caaattcatt atttctaaga tacgagcttt ttcagcactt actttttcat caataatcgc
    17281 tttacgttgc gctttagtcg tcatttttgt atacaccccc ctttgttttt tgaaaaattt
    17341 gacctaacga tgcgcgtgac tccccgctac cctatctccc cacgcgaaaa aattttgaaa
    17401 atgaataggg gggcttcaat tgaaatacga aggaaaaact tttttgtctt ccgtttcgtt
    17461 ttcaacaatt ggatgacatt ttgaacataa tagcatgaga ttgtttggat caagcttaag
    17521 cagttcgttg tctttgattg gtacaatgtg atggacgtgt gctctctttc caaatatgaa
    17581 ctgaccacac cgctgacaat gaccaccttc tctttcataa ataaattgac gcatatcttt
    17641 ccatgcttgc gttcgataga atggtttgtt ctcatgatga taaacagact ttgcttgctg
    17701 cttcttcttg cgtgatctgc ttgatctctt gtgttcagta cagtagatac cctttgctat
    17761 tttgttcgtg catccgtcaa actgacagta tttcattcta cttcacgaat aagatttatg
    17821 atatcgcctt ttgcacggat agcaccagga atatcaatac catgtttctt agcatatgca
    17881 cgcaattctt ttgcagtcat attgtccagt tcatccgtat cttcagtgga ctgatcatta
    17941 gtagcttcgt ctccatcaaa attagcagca gtgtttccat cgctatcaag aatttcactg
    18001 ctatgagcta tgagttcgcc attgacagca acaaatgatt tgccatcact caaaattcct
    18061 ttttcttctt ttacttcaaa atttggttct tgatctttcg gaacaactac tgtccttttc
    18121 ttttctgaat cccaatactc tgttccacta atggatgttc taattttgat cattgccatt
    18181 ttaattctct cctttgtaat ttgtacggat tacttttgcg cccattcgct cataccattc
    18241 aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa gaaataacta acgtattcat
    18301 tccagttctg taatcatcat tctccacttg cacatgtgca tgattaccat tccaattttc
    18361 tctgtacgga ctattctctg cttcaattgc tttcttataa actttagcca aacttgattc
    18421 tacttcaata ctaagtaccg cttcatgaaa atcattcatt tgtttactct cctatcagat
    18481 attttgtggt atgatttata aaaaagatgc gaggtgtttt aatgcttcaa gaggttaata
    18541 ttaagtatcg tgatcgtcaa aatacagaga caatttattt gccaaatagt tgtccgcatt
    18601 gcggaaatat aatgactccg tatgtgtatt ttggggtttc atcatatagt gcgttcgata
    18661 atgatagaat ctttggcgta ttagtacagt gtataaatga tgagtgcaac aaattttata
    18721 gtttacaatt tataaatacg tttaatgaag atgaaggata ttctgaaaat attttaatta
    18781 aagattatta ccgtccgccg attaaaatag atttaccaga aaatattgaa aaagtttctc
    18841 ctacatttgt tgatatttat actcaagcca cacaagctaa atctgagcaa ttggaacaaa
    18901 ttgctggagt tggttataga aaagctctag aatttctaat taaagattat gcaatatcta
    18961 aaaattcaga ggatgaagat aaaataaaaa aaatgttatt aggtaaaaca attgaaaatt
    19021 atttgaatga ttttcctaaa ctccaaagat tagccaaagc tgctacttgg attggtaatg
    19081 atgaaacgca ctatgttcgt agacatacta ataaagatgt tcatgatatg gatgcattta
    19141 ttaaatcagc agcgcaattt atcgcggctg attatgatgc tgatttagct gatcaattta
    19201 taaatggaaa taactttatg ccaactacta attaaaagaa taaaagagcc acagatacca
    19261 attaataaaa cggttgggat atcttggctc tttcatattt ttttgacact aatagaatat
    19321 catgctgcta acaatgttta catagctaca atgaccgaac atttagtgaa cagtgtaatt
    19381 ttagaataaa gagagttgtt ctttgtactt ctcgctattg taaaaatcaa tttcgttttc
    19441 ttttttcaat cggtcagctt gtctgtgttc atactcatct aaaaagttca atgtctttct
    19501 aatttctgca tgtcgctgtc tgacatacga tgagctatac ccaacagatt cagcaatatc
    19561 ttctaagctc atttgatcaa catatttcat cttaacaatt tgattatcga taccagaaaa
    19621 gctatctatc agcaataaca tttcttcttt ttgctcgatc aacaattcca attcattttc
    19681 tatcttttgg atattctctt ctaaagaaga agttctcgaa ttcttttcga tgcggacgtt
    19741 tgctaaatca ccacagaccc atcgattcaa ttcaagctta cttttattaa gattccactt
    19801 taagtaaaga atctgctcat ctagttcttg gtaatctttt aaccaatgaa atctcacaaa
    19861 cgccacccct tatggtagaa tagtattgcg ctgctatctc gaaaggggta gcttttttat
    19921 tttttcttag ccgttccatt ctggattgcc ttttgttcca aacgtcgttt tttcttctta
    19981 atctttgatt ttgtttttcc catacaattc tcctttccga aaacacattt tgttcgattt
    20041 ttaatactaa ataaaagttg gccagaattt ttcgttcgtc tttactcatc tttttttgaa
    20101 taagattact agcataaaaa tagttagtac cacgaaaatg aacaggtaaa tcaattacgt
    20161 tcaccttctt catgccagtt atcagccact agaatctttg cgttagtgcc gccaccgttt
    20221 tcttttttgt atttttcaaa tgctttataa gcagctgttt ttgatttata gatttttccg
    20281 gaaccgactg ggaacatcca tgtgccctct gtgatagcta agatgtatac tttcatttaa
    20341 ttcacccctt taatccccaa cactacatat ccggacttct gcatgtaatc agtaatataa
    20401 gtgatctctg ctttgtatga gtcaccagtg taactaaatg ttggtctcgt tagtccaggt
    20461 aattcatgtt taatgtattt tttatattca cgtagtatta actgatcacc aactttgaag
    20521 tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc tcgtcactgc ttcaaaatat
    20581 tctggtagta tttttagttc gtggatcatt ctgtttcctc cttcaaaatt cgctcatcat
    20641 caaaaagtga atctccacaa aatccttctc ccaaatattt atcgttcatg tataagtcaa
    20701 ccaaatattt gtgatctgct tctcgatatt cattaatatt aacgatcttt attttgtaaa
    20761 cattaccttc gtcgctcact atcttgaatt gttctctgac tttcaccacc gttcctcctc
    20821 tttttgaaaa aattttagtg tttttgtaag accccctaaa tcagcttttt tatttgatat
    20881 aatacttgta tcgattaatc aaatcgaaat taaatttaaa aaggtgattt atcatgaata
    20941 tcaacaattt agtaaaactc gttgaacaac gttttaaaga atcaggatta gaacaattaa
    21001 aaaaagataa ttctccagaa ttattttcta attttaccga agacgacatt cctactctta
    21061 aacaattaaa atattattct gattattgga cgcaaactcg tgagaatgaa ttaaatttag
    21121 aaaaactcat tattaaaaag aaatagcaac tccagcccac acggctggtt ttttagtttc
    21181 aacttccctc cttacgggtc aaaatcatcg gttccggcat aatctgatca tcctttagca
    21241 acaaatcagg atattttttc cttagcttct tattgttcgt gtaagttttc aatagccatt
    21301 ggttggcttc gcttttgctt tctccagaaa aataaatttt ccctgtttgg atgcctgcaa
    21361 gttgaatcat cattttcacc tcaaatcaaa tttttttctg ctagatggag caaaagcaat
    21421 ccgacatcaa gaatcttttg acccattatt tcagctactt tgctcggctt aactccttga
    21481 gaaaagagca agcgtgcttg ctctatctct tcgtgagtcc aaataaattt ggcttcctca
    21541 aggataatta ctggatcagt tgctctcatt gttcctctgg aaaaatgatg tcattcttat
    21601 gcttggacca ggtatctgca tatggaacaa agtattgtct agctaattcg atttgatcaa
    21661 ttaaagcagt ttctgataat tcatggtctg cagcaatttc attcatacta tcgccttcat
    21721 ctaaacagat aagaccgttg acgttatcga tttttacatt tgtgactgct tgactttctc
    21781 cacgctcata atattctaca gttgatactg atacatcagc ttcggagttt aattgcagaa
    21841 tctgttactg ccgggataat ttaatattct tttcataatt cacctttttt cctactgaca
    21901 tcgaatattt ttcatgttat gattagccgt gaaaggagag ctatattatg tgtttacaca
    21961 tgatgtcctt agctcattcg gttctttgga agcttttttg gaaataactc tcaggttctt
    22021 gacgcaaatc tcatttattc tttgaaacaa aaaacttcca ttttccgaga gctttggatc
    22081 acttcaaaag agatttgcat ttatatttct gtgaccttga gtcgcgtaaa gaaccttaca
    22141 gcaatggcgg caattccact ttcaggaaac cgcctttttt cattgatatt ggtggttagc
    22201 ggactaccca ttttctttcg gcagtgccaa accacaaatg atctgtgttg atgtttaggt
    22261 agttgtatac cagataagct aaatccaaag tgcctgcatc ttcaagacga caatattcca
    22321 aaaattctcc catagctgga cataaaaatc ctggagctat catatcccaa cagttaatag
    22381 tgcaaagaaa ttcgtttagc ttctgaacgt tttctttggt ttcacgaatt acaacatact
    22441 ccaattcttc tccgctggca gagcattcca aaacttcaaa ttgattaaga aatggtagaa
    22501 catatgcttc aaacatcaac attccatctt tctgtttttg cacttgtttt gcatcaatca
    22561 tgtctatcct cctattcagt tatttcttcc attaatggaa ttatttcagg tcactggact
    22621 taacgaaaac accattgacc atctttccag tccgaccttt gatttcgtca taagcaaaat
    22681 ccaagcactc gtataaatcc atatcatttt gcattgccaa gatgatcaag gttactacta
    22741 catcgccaat tccgtctctt aaaccatgtt catcttttct agctagagaa gcggcaactt
    22801 ccccgatttc ttcgaccgtt tttaacattt gcttgctgaa atccgcttga tccaatccct
    22861 tatcttttgc ccactgctct actttcgcga ttagttcgtc cattatttct cctccacata
    22921 cctaaactgt cttccttttg aatcaatcca taagctccta gctctatccc aaataatatt
    22981 tttgctcaga ccagtaattt cagataactg tttagcggta cccgttacta gaattcgatc
    23041 accatgccag attgcaatct ttctcggcgt tctccgtttg ggcttttcag tccacattga
    23101 tttaccgagc ttttggactt ctgcaactat ttttttatct tcctgccaat cttctgaata
    23161 agtcaattcg ataattcgct gcattgccgc tttcttatcc atcccgacat tctcctttca
    23221 gtaatttgag tacttgatca agtgcgctct cacgtccgcc atgaaatgtg ttgagccact
    23281 tgtcttcgta cgacacactt tgtcttaaag cttcttgatg cattaattcg atctgtgctg
    23341 taaatgtctt taggtccatc ttgtttacac ctgctcaagt tcattaagat gtttttgcaa
    23401 gcctttaaca caatcaatga atagtaattt ggtatatgct aaatttctta attgcgttcc
    23461 atcgatatag agtgcgaaat agtatctgag tttactccaa ctcgaacgat cattcttaat
    23521 tccttcaatt ccagcttctt cgagttgatc atatacgtct ctcaggacgt ctaattcctc
    23581 gcccttttta taattcgcta tttcattaat tagttctaga taatcgatct tcatttttcc
    23641 acctcttaga atggtgcttt tgattgtcta ttagctcgtt ctagcgcttt tttcttgaga
    23701 taggcttctt ggtcgattgc ccattcagga agcttctctc gtcttcctgt gcgcttgtat
    23761 ccactgcttg cgttcttagg ttcacttttt tctttccttg cccaacttcg aatagttgcc
    23821 aaatagtttt tataagtctt accagatgat tcacaatact ctgacagtcg ttcgattcgc
    23881 tcttggtaat cattagggaa ttctgttttg agtttctcca tctgctcatc tgacaaaaga
    23941 acatttttat actctccgta tttatgacgg acgggcttag ccttcgattt tttcgaaggc
    24001 ggtaactctc ttatatattc ttttgtatta ttaaatgtat tattaataga tgtattatta
    24061 tctttgactt tttcgtcaat aggggtattg cgtttttcgt caatagggta ttgattaatt
    24121 cgtaggtacc tattgattat ttgattggta ccctctttgt aaatgatttc ccgattcaag
    24181 tatccaaact taatcaaatc acttacccat cgcgatatgg tctctttatt cacaccatat
    24241 aaatctgcaa agtactcatt gcctgcccaa caaaagcctc tttcattaca caaggccgtt
    24301 atctctccgt ataacaactt agtatttggt ttaagtcttt tgtcgtacct tacgttggct
    24361 ggtataatcg cataataact tcgatgttct gtcattttta ccctccaata tttaactttt
    24421 tgattgtttt ctggtttaat ttgatccctt tgatttgata tttatttttg aaattaatca
    24481 cacctatttt gtgcttctct gtgtgatgga ttctgcagag tgctgcaaat gtgtactctg
    24541 catgatcaac ttctttgcgc tttcgtcttc ctagcgcttt gtcaaagtga tcgatgtcag
    24601 ctcctgtttt gccacagatg cagcagactc tttttgtgat gcatttgtag aagtaatatt
    24661 cttgattcgc aggtaaaatc tcatagcctt ctttgaaagg aatatgatgt tcaaagatga
    24721 aatctaagat gatatttgct aagacattgg catcactcac agttgtattc gattcgtctt
    24781 tcaggcttat tttgcgccct gtgacacctt caaaacgaaa gtagaagaat tccttccaga
    24841 agtccgttgg catgcctgta tcgataaaaa tatcgcctat cagcgcatag atgaagtttc
    24901 gttgctgtac ggtgaaacgt ctaggatcaa taaaacgaat ttcaatgact cgatcaccat
    24961 cgtagccgtc atacatcgtc tttagtcgat caatgttcac ttcctcattg atggttgcgc
    25021 ttatgtcttt tcctttgaac tttttcagaa ccgctgaata tgaatcgatt aatggtttaa
    25081 acactcatat cacttcttat ctaattcttt tctcttagct gctattgctc gctccatcaa
    25141 ggcacattgc tcatagctta actgttcaat agtttcaacg ttatcagcta agagccctaa
    25201 tttatctgtc tgctcattaa catattcgat taaggttttg gtcatatctt tacccatctg
    25261 ctcattgaaa gcttctagaa tcgtctctag catgtttaat ttctttgtat cgattctagg
    25321 tggtgttgga atatcttccc cttgaaatac atataatccc agtccgtgta gagccaatgc
    25381 tttcacaaag catcgcttca atgagttatt gatttgcatt gcatttggtt taacaactgg
    25441 ttggtttcga taatctaaaa caggaaataa ttcggtttcc gtgtgtcctt taaccgttac
    25501 tgagacagat acataagtcc cagtttcatc cataagaaaa ggtttatatt cctcaacaag
    25561 aaagtcttga tgagttccag aaacaaccct gtagtgtttg tactcattaa tagttaccgt
    25621 tgcctgtgga tcattctttt tcataatctc ccacgcgtga gcccaagata aataatcaaa
    25681 atttcctttt ttcttgagaa ttttatttaa cttacgacta aaaagttttt caaagttcgt
    25741 tgtccctttg atttcactca tcaaattctg cctccatttc agcaatgtat ttcttacctg
    25801 gtccgtaata agagatatca atcaagttat ctctgtcgta ctcttctagc gcatcaatca
    25861 agccatcttc gatgacgtaa atatattcag gtttattcga atgcttcgat agatggataa
    25921 gataaacatg atcccaaata gttacatggt tgcctaaatc atcttgatcc caagctagtt
    25981 cttcattcgt caaaagattt cgtctgattt ttcgaccact tgtttcctca attttcggct
    26041 tgccccaatc aggatcaatc aaatattgat ctagagtgga aagttctttt tccatatgct
    26101 aaaatctcct tatgatgtgt tttctttgtg actctttgct tgccggcgga gtcacttttt
    26161 tatttgttgc catgcttttt gcttgtcaat atgttgttgg cttaggatga ttggtttata
    26221 gtatttccac cagcaattag caattgccgt ccctattctt agcgcttcag ctctattcat
    26281 tgtcatcacc aaaaagtctc tgttgtctgt tcagttgatc aatttccata cggatcgcag
    26341 tttctggcaa ccacatttca ataaatgaaa cagcatcatc gaatctctta cgaggtaact
    26401 cgccatatct tgggattgaa aaggtacgtt taaattcaga ccaaaatttt gagaatactt
    26461 ttttgctgat ttcttcataa gctcggcttt cttttccccc tagaactccc ataactttca
    26521 tatttccttt ttgcttaatt tcaaactctt gttgtccgct aattcgcata gtatctttaa
    26581 gcatggaaac atctttttta acatctttca tttcttctaa ttgatagatc atcatatctt
    26641 caattgtttg aggaacagta ttcttgcgaa taacatcttc catttcgttg aatgcttcaa
    26701 tgtatttttg tttgaagtag atagctttct ttcctgtaaa ccccatagcc agcaagaaaa
    26761 atccatctct actaatgaag aaaactcgtc gatttctgcc atatgaatct ggttcattac
    26821 cttctacaaa catctgtcca aaattggaca catctttttt tagtgcatca atatctctta
    26881 aaacatgttg atgttttttc tcgaagcttt ctgccacttg taagctcgta gtcacagctt
    26941 ctttattttt caaaattact aattcttgca ttatttcttc tctccttttt ggtataattt
    27001 agttaaaaac tggtggtgtt tattttgaat ttttttcact taacaatttt tcaatggatg
    27061 tcaataataa gttttatatt gagtacgttt ttaacaggga taaaaataaa aggaatgcgc
    27121 cctcaactag aagttgaact taatgcttct tacttcgctg cagatatgat ttatacaaaa
    27181 gtaatcattt ctaactattc aacagaacca gctatgttag ttaatttaga actttcatcc
    27241 gctaacttaa accgcagatg gtcagctact ccatttaaga aattaattgc taaaggagga
    27301 tcagtcgatg ataatagaat atactctgaa gctgtaccgc taaatattcc tcctaaaagt
    27361 gctatatctt gttaccttgc atttgaagta gggaaaatta attttaataa actccttaat
    27421 caccaaacaa aaatgatatt tactctaaat agaacccaaa tccacaaaat tgttgatatc
    27481 aaaaatacaa acttcccaat agagaaatta gtgaaagaat taaactgagt aatagtaata
    27541 tacattctag tttctctttt ctattcattt ttagtcagcc ccctcggttg gctttttcgc
    27601 tctgtactca gcttcagctt catcaagccc cataaaaatc caaaccatgt aaacaatcgt
    27661 tcctatcaac gcttgcctgc ttccccaaag tcctaaagcg tagatgatta gcggtgcgct
    27721 gaacactagc gctctattaa attttcccat ccgcttgcct ccttaagatt tccgaaaaat
    27781 ttgtttctaa aaattcaagt gttttacttc ttaaaaatag atatgtgtct cttccttcaa
    27841 ctggataata gacaaatcca tttttgtttt tctcgatatc gataatattt ctatatcttg
    27901 ggttctttaa aactctagaa gtaaaccaat catattttct gttaatccgt tctagcactt
    27961 ctggcaacgt catccatcta ccagtatcat cagctttttt taactcctca taatccactt
    28021 gggagataat tacatagcct tctggaattg ggatttttgc ttctagatat tgcattagct
    28081 gttcctccct ttacaattcg tacatagtga taatcgaatc tataattctg tttgcttctg
    28141 cagaagtctt tttaccgttt aaaattaaag ataagtagct tttgctaatt ccaaatcttt
    28201 cagcaagcat ggtgtaagtt aagaactttg aactttcgac atattctttg attttttctc
    28261 tatctcgttg agtgatttct gcaatgtcag tcatactaaa actcctttct aaccaatttc
    28321 ctctaaatcc atttgagggt aatatccttc tttttttagt aattcgtaga taaatagacg
    28381 ccctttctgt gtccatttgg tattcattac aattttagtg ccaccatcgg ctttcgggat
    28441 ctcagttgta tgagattttg tgtatccttg gttcatgtgt tttttgcaca ataaccattg
    28501 gttaccgact tttttctgaa tacctagttt atgaagtaat ttattcatct gttgtggaga
    28561 catcccataa tctgctgcaa tctgactaat tgttactgaa tctgtagaag ataatatgct
    28621 atctaaatag gagattttag gttcgtactc tgcaatcttt tgttctgcga ttaatcttcc
    28681 agttctttct tcttttagtt gagttgctaa ttgaatgatt gtatctggat taagcaaagc
    28741 ttcttctact ttttctggag ttagataacc tccatgtttt ctaattgctg gcaacacttc
    28801 acttgttacc caacgtttaa attttttggc agagggaagt tttgatttta agattaaact
    28861 gtacaaacca gattcgttga tgattgtcat ctctcttgat tgacctgagg tcgtgattcg
    28921 cgaccccatc ttatcttcca aatctacatg ccgcgacaaa gcatctttag tgtttgaata
    28981 acccaaaaca ctcgcaacgt ctttgcctac aaaatatggt tcatcattta ctaaaattgt
    29041 ccgaacttcg ttttgttcga aattaaaaat ttgcggtgtg ttcatatttc tcattccttt
    29101 cttttatatt cgtaaacaaa tttaacaact tttttctaaa ttcaattgac agtatctgag
    29161 taatactcta tactaagagc ataattaaat aagacataaa aacattgatt ttaaagcttt
    29221 cttggcggtc tgcatttaca tatcaatagt gttttttgtt gtctttttag ttgttaaatc
    29281 tgtttacaag atatagtata gctcattact cagaattagt caaattattt ctgcgtattt
    29341 ttctaaactt tttttgtaaa catttagaaa ggttgtttta tcgatgaata cctatgaaat
    29401 aataaaagac ttggcgaaga ggaaaaagat atctatacgg cagttagaaa ttaattttgg
    29461 atattctaac ggttatctag caaagtggaa aaataatact cctaattcag atgaattaag
    29521 tcggattgct gactatttcg atgtctctgt agactacctt ctaggtagag aagaaagaga
    29581 aacccctaaa catgtggatt tatcagaaga cgatactgtt ttttcttttg atggaaaaga
    29641 aatatctaag gagacaatgc gtaaagcgat tgcaattgct aaagctttag aggaaaatga
    29701 atagttggag tgatggtttg tatgtatttg aaattgaaag aaatgttgag cgaatacaat
    29761 ttgaaagtag tttatatgga gatgaaagag cctggtttct actatccaaa acccagaatc
    29821 atttttttga atgaaaatct atacggtgaa actgcagaag cctttcattt atctcatgaa
    29881 cttgcacatt tcagcgcctc tcattttgaa ttttcagttt tgtacgatac ttctacaact
    29941 tttcactcaa aatttgaaac agaagcagat aaaatagcta ttctaatttt attaaatatc
    30001 tatattgaga acgaactgac tgatgaatct cagtttaatc tcgaaaaatt tatggagtat
    30061 tattctattc agaataagct cagatacact tgttatgctg tctgccaatg ctattttaag
    30121 aaaaaatatt cttacgcaag gcaatatgta tgaatacatc aagaattatt aaaaaaaccg
    30181 atttcagcaa aacattaaaa aagcccgtgc tgcaacacgg gctctttcct catatatgag
    30241 cttctacaaa aaaaatcggc tctttgtcaa taaggactga tgggttgtac aaaatcaaat
    30301 ctgggtcaga atgaacctca ttctgg
//
LOCUS       DACDYJ010000030        29572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDYJ010000030 DACDYJ010000000
VERSION     DACDYJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.030"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(47..1342)
                     /gene="purB"
                     /locus_tag="IWM51_001398"
     CDS             complement(47..1342)
                     /gene="purB"
                     /locus_tag="IWM51_001398"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP9970825.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1355..2488)
                     /gene="purK"
                     /locus_tag="IWM51_001399"
     CDS             complement(1355..2488)
                     /gene="purK"
                     /locus_tag="IWM51_001399"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP9970826.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2472..2960)
                     /gene="purE"
                     /locus_tag="IWM51_001400"
     CDS             complement(2472..2960)
                     /gene="purE"
                     /locus_tag="IWM51_001400"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP9970827.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3376..4683)
                     /locus_tag="IWM51_001401"
     CDS             complement(3376..4683)
                     /locus_tag="IWM51_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP9970828.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4686..5267)
                     /locus_tag="IWM51_001402"
     CDS             complement(4686..5267)
                     /locus_tag="IWM51_001402"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP9970829.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5383..5480)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5839..7131)
                     /locus_tag="IWM51_001403"
     CDS             complement(5839..7131)
                     /locus_tag="IWM51_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9970830.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7128..7751)
                     /locus_tag="IWM51_001404"
     CDS             complement(7128..7751)
                     /locus_tag="IWM51_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970831.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7754..8590)
                     /locus_tag="IWM51_001405"
     CDS             complement(7754..8590)
                     /locus_tag="IWM51_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9970832.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8607..9494)
                     /locus_tag="IWM51_001406"
     CDS             complement(8607..9494)
                     /locus_tag="IWM51_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9970833.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9510..10793)
                     /locus_tag="IWM51_001407"
     CDS             complement(9510..10793)
                     /locus_tag="IWM51_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9970834.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11476..12159)
                     /locus_tag="IWM51_001408"
     CDS             complement(11476..12159)
                     /locus_tag="IWM51_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9970835.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12177..12794)
                     /locus_tag="IWM51_001409"
     CDS             complement(12177..12794)
                     /locus_tag="IWM51_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP9970836.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12804..13628)
                     /locus_tag="IWM51_001410"
     CDS             complement(12804..13628)
                     /locus_tag="IWM51_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP9970837.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13621..14181)
                     /locus_tag="IWM51_001411"
     CDS             complement(13621..14181)
                     /locus_tag="IWM51_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970838.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14355..15311
                     /locus_tag="IWM51_001412"
     CDS             14355..15311
                     /locus_tag="IWM51_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9970839.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15616..16572)
                     /locus_tag="IWM51_001413"
     CDS             complement(15616..16572)
                     /locus_tag="IWM51_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP9970840.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16676..17278
                     /locus_tag="IWM51_001414"
     CDS             16676..17278
                     /locus_tag="IWM51_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9970841.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17366..18865)
                     /locus_tag="IWM51_001415"
     CDS             complement(17366..18865)
                     /locus_tag="IWM51_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP9970842.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18881..19405)
                     /locus_tag="IWM51_001416"
     CDS             complement(18881..19405)
                     /locus_tag="IWM51_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970843.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19703..20452
                     /locus_tag="IWM51_001417"
     CDS             19703..20452
                     /locus_tag="IWM51_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP9970844.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20443..21228
                     /locus_tag="IWM51_001418"
     CDS             20443..21228
                     /locus_tag="IWM51_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9970845.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21197..21913
                     /locus_tag="IWM51_001419"
     CDS             21197..21913
                     /locus_tag="IWM51_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9970846.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22015..23034)
                     /locus_tag="IWM51_001420"
     CDS             complement(22015..23034)
                     /locus_tag="IWM51_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP9970847.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23053..24111)
                     /locus_tag="IWM51_001421"
     CDS             complement(23053..24111)
                     /locus_tag="IWM51_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP9970848.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(24332..25480)
                     /gene="nagA"
                     /locus_tag="IWM51_001422"
     CDS             complement(24332..25480)
                     /gene="nagA"
                     /locus_tag="IWM51_001422"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP9970849.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(25556..26392)
                     /locus_tag="IWM51_001423"
     CDS             complement(25556..26392)
                     /locus_tag="IWM51_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9970850.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(26576..27793)
                     /locus_tag="IWM51_001424"
     CDS             complement(26576..27793)
                     /locus_tag="IWM51_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9970851.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            28176..28259
                     /locus_tag="IWM51_001425"
     tRNA            28176..28259
                     /locus_tag="IWM51_001425"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28210..28212,aa:Leu,seq:gag)
     gene            complement(28499..29497)
                     /locus_tag="IWM51_001426"
     CDS             complement(28499..29497)
                     /locus_tag="IWM51_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970852.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         8843 a         6304 c         5064 g         9361 t
ORIGIN      
        1 tatttttgct attctatttt aattgaattt tgcaactcac tcttctttat tccaaaccta
       61 ctcgtttgaa tatcacgtca acgtttttca aatgatgatg ataatcaaag gcatcgtcta
      121 tttcttcttt agataatacc gatgtgatgg ttggatcttc ttctaacagc gggcggaact
      181 gcacttggtt gtcccaagcg tacgcagttt ttggctgaac aagatcataa gcttcttctc
      241 ttgtcattcc cttatcgatc aatttcagca taacgcgctg gctataaatc aaaccaaatg
      301 ttgcagacat attccgcttc atgttttcag gaaagaccgt tagattttta acgatattgc
      361 caaaacgatt taacatgtaa ttcaataaaa tcgtggtatc aggcaagatg attcgttctg
      421 ctgaagaatg agaaatatct ctttcatgcc ataatgtgac attttcatag gcagtgacca
      481 tgtgtccgcg caccactctg gctagaccag ccatattttc tgaaccgatc ggattccttt
      541 tgtgcggcat agcagaagaa cctttttgac ctttcgcaaa aaattcttct acttcacgtg
      601 tttcggattt ttgcaatccg cgtatctctg ttgcaaaacg ttcaatactt gtagcaataa
      661 gcgccatcgc tgagaaatat tcagcatgta gatcccgagg gagaacttga gtagagattt
      721 cttgtggacg gatccccaat tgtttacata catactcttc aataaatggc gggatatttg
      781 cgaacgttcc cactgctcct gagatttttc ctgcttctac tccttttgcc gcatgttcaa
      841 atcgttcgat gttccgtttc atttctgaat accatgtggc aagtttcaat ccaaaagtag
      901 ttggttcagc atggacaccg tgtgtccgac ccatcatcac tgtatatttg tgttcttttg
      961 ctttttcgcc aatgatttcc gtaaaacgtt tcaagtcttc ccgtaaaata tcatttgctt
     1021 gcttgatgag atagccataa gcagtatcta cgacatccgt acttgttaga ccataatgga
     1081 cccattttct ttcttctccc aatgtttcag aaaccgctcg tgtaaacgca acgacatcat
     1141 ggcgtgttaa tttttcgatt tccaagatcc gatcgatatc aaatgatgca tgctcacgaa
     1201 tttttttcac atcttcttgc gggatttcac caagttctgc ccaagcttca tctgctaaaa
     1261 tctcgacttc gagccacgca cggtatttgt tctcttccgt ccagatattc cccatttcag
     1321 ggcgtgtata acgttctaac atgttttttc tcctctaatc ccaaatattc gtttgtttga
     1381 tttcttccag cgtttgctgg atgtcttctg tcaagatagt gatatgtccc atcttgcgac
     1441 ctttttttgc ttgttttttt ccatagaaat ggaattgcca gtctggctta ttctcaatca
     1501 agcgatagct ttccagcagt tgttctccca aaacattgac cattactgca ttgcttatca
     1561 aacgtacttt ttctcctaat ggccaaccac aaatacctcg aatatgggca tcgaactgac
     1621 tcatagaaca agcttcaata gaataatgtc cagaattgtg aggacgagga gctaattcat
     1681 tcacatatag cccgcctgtt tttgttaaaa acatttctac ccctaaaaca cctgaaaggc
     1741 tcactgcttc tgcaatcaca cgggcaatac gctcagcctc ctcgatcaca tcctgatcaa
     1801 tcgcggctgg agcaatcgtt tcatgaagaa tattgttgtg gtggatgttt tccacgaccg
     1861 gaaaagtcgt gaaatctcct tgaccatttc ctgccaccat gatcgaaatc tctttttcaa
     1921 acgggatcca tgcttctaaa acacaggttc cttctcttaa aaggtccatg gcaggagcta
     1981 gatccgcccg acttttcaaa acatactgcc ccttgccatc atagccgcct cgagtggttt
     2041 tcaaaacaca aggatagccg atcccgtcaa tcgcatcttg gatatccgtc ggactgacga
     2101 tcgttgcata aggagcaatc acgatgttat tcgtttccaa aaaagatttt tctaacaggc
     2161 ggtcttgcgt aatcgcaagc aagtctgtcc cttgaggaat aaatgacatg ggtaaaatcg
     2221 catttaacgc ctctacactg acgttttcga attcataggt aatgacatct gttcttctgg
     2281 caagctcttt cagcgcaaag gtgtcatcat agacactttc aatgtgccaa tctgcaactt
     2341 gtgcggctgg acaatcgctc acaggatcca gcacacctac tttaaagccc atttcttttg
     2401 cactaatcgc catcatacga cctaattgac cgccaccgac gatcccgatc gttgaaccag
     2461 gcaacaaagg tttaaccaag ttgatcacta ctttctataa cagttttagc caacattgaa
     2521 cgtctttcag ccaatttagc ctccaattct aaatcataca tggaaagcat ctgtatcgca
     2581 agtagtccag cattaattgc tcccgctttt ccgattgctg tagtagctac aggtactcct
     2641 ccaggcattt ggacaatcga aagaagcgaa tccaaaccat ttaatgttcg tgattggacc
     2701 ggtacaccga taaccggcaa agttgttttt gatgcgacca tacccggcag atgcgcggct
     2761 ccccctgctc ctgcaataat gattttgatt cctttttttc gagcgttttg tgcatattca
     2821 aacatcaaat ctggggttcg atgagcagaa acgacctttt tttcataaga cacaccgaat
     2881 tcatccaata tttgacatgc ttctttcatt gtttcccaat ctgaagtact ccccataatc
     2941 acagagattt ttgctgtcat tgtttttcct cccagttttt ctacattcat attttaacaa
     3001 tcactttttt tgatgtcaaa gaaatttcga atgttaacaa agaaaaacat tttattgttc
     3061 gtgtttatta aagataaaaa aaaggaatgt gacaaaagtt aggtcacatt cctttttatg
     3121 gataaacggt gttctcctgc caactcctcg gtcacaagga acaatattca acaaacatga
     3181 gaagcttttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
     3241 ttccttcaac ggtgttcaat cagctgaccc tcggtattag ctcaaacaaa cggcaaaata
     3301 cgaggaacta tttttccgtt tgtttctcta acaatcaggt caaaacgctg atttcacaac
     3361 ctttcttttg ctctattact tcttgaaggc ttcttccaat atttcttcat cttttttcaa
     3421 tccattaaag atcaaattca atatgatagc cgtaatgcta ctcattacga tgccatttcc
     3481 agtgaacatt tgcaatgttt gcggcatttg actgaataat gtcggcgtaa cattgaaacc
     3541 cagcccaaat ccaacagaaa cagcaacaat caatagattt ttatcattgc tgaaatcaac
     3601 ttccaacagc atacggattc cttggactgc caccatcccg aacatgacta acattcctcc
     3661 accaagtacc ggctcaggga tgatttgtgc taacgcaccg attttaggga ataatcctaa
     3721 aacaatcaag aagaatgcag agaaatagat gggttttctt gttttgattc ccgaaagttg
     3781 aaccaatccg acattttgag aaaagccagt ataagggaat gtattgaaaa taccgcctaa
     3841 gataacggca agtccttctg cacgatatcc ctttttcaat tcttcttctc caacattttt
     3901 cccagtaatg tcaccaagtg caaaatatac acctgttgat tctaccatgc tgaccatgga
     3961 aatgatgatc attagtaaaa tcgaccatac gtcgaaagtt ggtttaccaa aataaaaggg
     4021 ttgtggaaca tggaacaacg gcgcatctcc aatcgcgctc agatcaatca ttcccatgaa
     4081 agcagctaag atgcttcctc cgactaaacc aatcaatacc gcgattgaac gaatgaaccc
     4141 ttttgccagc atttgcacgc ctacaattag gagaatcgtc acaaatgcaa gtagcagatt
     4201 tgttttgtca ccaaaatcag ctgctgttgc attacctcca cccattttct cgattgctac
     4261 aggaatcaaa gtcaaaccaa taacggtgat aaccgttcct gttactatag gagggaatag
     4321 ttttttaatt ttagaaaaga caccagaaac taacacaaca aagatcccag aagcgataat
     4381 agatccgtag atagctccta ctccttcatt ggttccgatc aagatcagtg gagctactgc
     4441 ttggatcgca catcctaaaa cgacaggcaa gccaattccg aaaaatcggt taactgtcaa
     4501 ttgcaataat gtcgctaatc cacacataaa gatatcaatt gaaatcaaat aagtcatctg
     4561 ttcttgatta aagcctagtc ctgtaccgat caaaagaggc accgctactg ctcctgcata
     4621 catcgctagc agatgctgca gccctaaaac cgcagctttt ccattttgtg tttctttttc
     4681 cacgattatg cgtcctcctc aagaaactca acttgtccat tcgtcaagga agcaatacgc
     4741 gcaagagaaa cgactctcaa tcccatttct tcaagtaact gacgtccatc ttggaaagac
     4801 ttttcgatga cgatcccgat tccttctact tgcgctccag cttgctggca taattcgatc
     4861 aatccttttg ccgcttgtcc gtttgccaag aaatcgtcga taatcaatac tttgtcttct
     4921 gaagataaaa atttacgaga aatagaaatc gtacttgtca cttgtttcgt aaaagaataa
     4981 acagaagctg tcaataattc ttcatccatc gtcaaacttt ttgcttttct agcgaagatc
     5041 attggaacat ctaattctct tgcagcaaat aaagctggtg cgatcccaga agcttcaatg
     5101 gtcacaactt tcgtgattcc ttgttcttta aatacttctg caaaccgttt tcccatttgt
     5161 tccatcaata cagggtctac ttggtgagtg acgaagctgt cgacttttaa tacgccctcg
     5221 cctaatatac gtccgtcttt ttgaatacgt tcaactaatt ctttcacttt atttgctcct
     5281 ttatcattta ttaataccga aataaccgct tttgtgcaaa aacctactat gtttctgtta
     5341 aagaaaaaca ccctccactt tatcataaga aaagaagggt gaaatagcca atcttttact
     5401 tatagtccag catttacggt actggctaga aactgtcatc catattatga cgatatataa
     5461 gtaggtatgt aaaactaata atagtgtaaa ttcattttct tgcaactgtt ttgcacgtga
     5521 aggcagtttg ccatatttga ataaaaaatg ttgtttcaaa tattgaaaac agtctaacac
     5581 tactttctga aactgacaac gtggtttact tatttttttg taaaatctaa tgatttttaa
     5641 tgcattattc ctttttgttc gggtttaaat agttattttt attttctcct atctaaaaaa
     5701 gcagcccaca taaaaagaag gcatgacaaa aagcatgtca tagccccttt ttccgaataa
     5761 atagtgttca aaagacagcc tatcggcaat aaggcaagat tttataaact acgggaagta
     5821 atttccgtaa atctattctt attcttttaa gctgacaagc tcgtcaatca ctactgcttt
     5881 tcctgaagca atagaatgat ttgctgcgat tcctgtcaaa atcgacaaaa taccatctac
     5941 gtgagttgct gcccgattcc acggatcttc ttctttttct tcaccaaata gatcatctaa
     6001 taaacgcgga tctcctccgc catgtccgcc cgcttcttgt tcgacgatta cctcatatgg
     6061 ctcaccgaac atcggataga catggatgga ctgaagaacg gctgctcctt catccttttt
     6121 atctccacct gcattgatgt aagacttttc gatcactttc atttcgatcc gtcctttgct
     6181 gccattgaaa gaaacattga atccttccca cggcatatac gcgtttagcg aatacgtcaa
     6241 aatcgcacca gtttcatatt tgaccgttac gcctagtgta tcttcaatag agatatcatc
     6301 ggaaaataca ctgcggtcac gaatatatcc actgtctttt tctgcttcta aatacatttc
     6361 tcgcaattcc tcattggctg acaggtctat agcaaatgga tcagctttag ctagttcgct
     6421 tccatagcat cgactgtaaa atatttctac tccgcgtttc tccgcattat gcatcccata
     6481 aaagtttaat ccaccgaatg caaatacctt ttggggacgg ctgttcaacc aaaaattcac
     6541 tagatcaaaa tgatgggtag atttatgaac aagaagcccg ccgctattgt gcttatttcg
     6601 atgccatctt ctgaaataat ctgccccgtg ttgcgtattt aacagccatt cgaagtggat
     6661 agaaaacaca tcaccaatca catcactttc tatcagttcc tttatcttcg tgtgatacgg
     6721 tgcataacga taattaaaag tgactcgcag gcttcttccc gtctgttttt ggaccgttat
     6781 tatttcttga gctttttcct catcgatagt cagaggtttt tcgcaaataa catcatatcc
     6841 ttttttcatt gcacgaatga tataatcatg atgcgttcga tctactgacg taataatgac
     6901 ttcagccggc cgtacgtcat ccagtagttg ctcaaattgt gatggcatac atgtctttac
     6961 ttttttctgt ccatacttgt tcacaagtat ccgatttgca taatccatcc ttgtttgaga
     7021 tacatcacaa aaaccgacta gttcacaagt ttgatgatac gtagaggcaa ttgcctcata
     7081 aaaaaaacgc gctctcccac caagtccgac ttgtacatat tttttcttca tttaaagtca
     7141 ttctcctttt cttttaagca aaatactgtg acaaatggca gccctggata cagtaatgca
     7201 gtgagtaaaa aatcatgctg tatacaccag aacagtccat acaaaaggca taaataccac
     7261 agaaacccct tgacgttttt tgacaacagc cagatattcc ttttgatcaa ataaacaaga
     7321 ctatctgctt tatcttcttt gcagatatca attagcgtac taaaaaaact atgggataca
     7381 aaaagcaaca gacacaatag aaaaggaatc agataccaag ctccaatctt tcccactaat
     7441 aaataccaat caactgcaaa aataatgaca aacagctgaa acaacgtaat gatgaaacac
     7501 atccgtttga tttttccttt cctccaacat accttttttt ctgccaagta atggacagat
     7561 agataataag aaggatacat catcaaaaaa taaatcagat agacaacacg agcagacagc
     7621 actaatccta aagtgaacaa acatcctaga actaaagcga aaagagcatt actgataaga
     7681 ctggttaaaa aaatccgaca gacatagtag ttgagcttgt aaagaaacat ctcttctatt
     7741 ggctttttca tattcagccc ttcagaccgc ttgtactgat cccctggaca agatatttat
     7801 tgaagaataa gaaaatcaga aaaaccggta gcagcgacaa cgtagacatc gcaaacatac
     7861 tcgcgtaagt cgtactagat ccagcgtctg caaataattt gatggcaatc gataccgtgt
     7921 aagatctcgg cttgtttaaa taaatcaatg gtcccatgaa gtcgtcccat ttccaataaa
     7981 actggatgat gatcgtcgtg atgatcgaag gtttcagcaa aggaaaaata attttggtaa
     8041 agacactata cttgctgcat ccgtcaataa tcgctgattc atctaactct ttggggatgc
     8101 tgaccatgaa ttgcatgatc tggtaaatga aaaatgcttg tccaaaaaaa tgtggtaaaa
     8161 tcagcggcac atacgtccca accaaattca gccgattgta aataataaac tgcggaatca
     8221 aaatcacttg tgcaggaatc atcatcgtta ctaacatgac gacgaaccag aatctttttc
     8281 ctttgaaatc gattctcgat aatgcgtaag caatacaagt cgagctcaga actgtcccta
     8341 tcgtcgaaac tgcagagatg acaaacgaat tcctaaagaa agtaaaaaat gaaattccag
     8401 caaaccccgc ccatcctttt gcgtaatttt cccattgaaa agtctctgga atcaaattca
     8461 gcgaaccact cataatctct tgatccgttt taaaagaacc tgcgatcatc cataagaccg
     8521 gataaatcat gactagacct aatagcaaaa taaaaccgta ttgaaaccat ttcgttactg
     8581 tattattcat ttcttcctcc tctcattcac acaacgaccc atttttttga tgatttgaaa
     8641 atcagaaagg tgacagctcc cataatcaac agcatgatcc acgaaatagc actagcatac
     8701 cccatagaat taaaagaaaa agcttggtta tacacataaa gagcataaaa gttcgtggca
     8761 tcattcggac caccttgggt aatgataaat gcctgcgtaa aagtcataaa cgccgcgatt
     8821 gtctgcataa ccaagttata caaaatgatc ggcgaaagaa gaggtaacgt gatcttgtaa
     8881 aacgtctgcc atttgttcgc tccatcgatc tctgccgctt cataatacga gcctgggatc
     8941 tctttgatac ctgcagcaaa gatgatcatt gccgaaccga attgccaagc agacatcaaa
     9001 acaagaggga tcatgaccag tttttgatcg ctaaaccagg aaagcgcagg tgcgcctaat
     9061 cgttccagga aaatattcac taacccgttt ttagaaaaca actcccgcca aactagagcg
     9121 atagaaatac tgccaccgat caaactaggc acataataca aagaacgaaa gaaagtcgtt
     9181 cctttgatat tcctcgttaa aagataagca atcaatagac caaagatcag tttcacaggt
     9241 acagatgcaa atacatagag aaaagtgact cggatggagt tcaaaaaacg ggcatcactc
     9301 agcatcttta tataattatc caagccgatc catgagactg gatttcccaa acgataatcg
     9361 gtgaacgaat aataaagtga caagatcatc ggtatcactg ttatcaatag aaatcctagg
     9421 atgaatggag aagcaaatac gtaaccgacc acattgtctt gatccaagaa ccgttttagt
     9481 ttttcttttt tcatgccttc ctcctgctgc tatcctagaa tgtttgacgc aaactcataa
     9541 agactctctg ctgcttcttc tgctgttact tcttcataaa tgactttttc aactaaaaga
     9601 ttgtattggt cttgaatttc cggattcaaa gcactatcaa agacattcac ttcgccgttg
     9661 tccatatcgg aaatcatttc taaataatca aatgtcgctt gcaaagttgc atcatctgca
     9721 cgagcgagct gttctcgaat attcgacatg attggtacac cacgttctcc ttttaaaatt
     9781 tcattggctt cttcactgtt ttgaaagaaa tcaataaact tagcagcttc ttctttatgg
     9841 gctgagcttt ttgccatact cagcatttgc gaagaacgta aagggatacc agattctcca
     9901 tctgcttttc tgcgaggtgg attaatcaac tgaatctcca tatcttcagg agcccctgac
     9961 aatatttctg tcaactggtt acttgataga aacaccattg ccgcttctct atccctaacc
    10021 gcctgaaaag acagatccgt cgtacttgca gctccaccac ctggttctgg atgggcccct
    10081 ttgtctacca gatttttacg cagctggaaa aaatctgtta gatagtctgg attttcaaat
    10141 cccaagctgt tagatagatc ttcccgatta taaaaattca atcctttctc aacttgtggt
    10201 atttgaataa tacagccagc tataaaatct tcgaatcttg aaatagcata gattcctaat
    10261 tcttcagtga tttgatagct gatttgttca aattcatccc atgtccaatt tgaagctggt
    10321 tcagggatat ttaattgttc aaatatattt ttattgtaag caatcgcaaa cgaattcacc
    10381 ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg ttgctaaata ggattcatct
    10441 gcattagaaa tatcaattat cccttgctcc acatattcat ctaatggttc aatctgattg
    10501 atgtattgag gaaattgatt gttcagttgg aaaatgtccc agatatcatc tgatgctgct
    10561 agtgtaccta attttgtgta atatccttca taatcataaa attcataatc gatcgtcaca
    10621 tggggatttt tttcttcata aagctcgatt acttttactg ttgcatcgtg tcggacttgc
    10681 gaaccccacc aagccatgtt cagcgtgatt tttttgtcat cttcttgccc acaagccgtc
    10741 aagaagaata acataacact taaagctaag atgattctca ttcttttctt cattctctgc
    10801 acctctactt ccattgttat ttttcaatat actgtatttt tatacagaac atatttatta
    10861 aacgatcttt ttcaagggga ataaaaacca agacttgcct tatcgttctg cctaggatga
    10921 acaactcaat actttcaaga aaaattagat agtgctgatt cttctttcat cgctaaaaac
    10981 acttcttgct tcaatgatgg gaggataatt acgcaaagaa aaaaggatat cagacaactg
    11041 cccaatatcc ttgacatagc taaataagca tgcttcttcg tgtgcgctct tccctaaaaa
    11101 acagaaaaat aactagtaat tctgtatgaa attaggtaca atatacacgt cgatgcacaa
    11161 gcattgacta tggcaacttg attggtcagt tcccgctgac ttttcaagcc ccgacaattt
    11221 agctgctact aaattgtcgg gtgccttttt tagtttttat tcgtttttca ttctatagaa
    11281 agcgcttcaa taaatcaaga catttttttg ttagaatgct aataataaca aaaggtttta
    11341 actcttctta tgtacattac aggtaaaaaa acaaaaaaat ttctcttttt tttgaattct
    11401 gaacaaatag tactggagta caaaagacga ggaagagcca ctatctgctc ttcctcgtct
    11461 tttgattctc acctgttatt catgattctt tttctcagtt tcccgatatt tttccatttt
    11521 acgactaaaa agctctccgc tacttggtgg cgtatatgtg atggcatttg ctcctgcttg
    11581 aatggtctca gcaatacttt cttccgttgg accacctgtc gcaatgatag gaagttcagg
    11641 atatttttta cgaaaatggc gtactgcttc tgcagtttct ttacctgcac tgatattgat
    11701 aatcgaaata ccagctgcta acttttcatc tatttctgta tatttcgacg ttaccgtact
    11761 gataactgga atatcaacta ctcgacaaac ttcttttact gtctctaaag gcgtaggacc
    11821 gttcaaaacg acccctaacg aaccttgcgc ttctgcgaac atgctcatgt aggaagaacg
    11881 agcaccttgt gtcaaaccac cacctacacc ggagaaaact ggaatgtcag ctgcctcaat
    11941 aatgctttta gtaatcgctg gatgaggtgt aaaaggatac acagcaatta ctgcattcgc
    12001 atttgtatta cgtatgatcg caatatccgt agtaaagatg atcgaacgga tcaattttcc
    12061 aaatatcttg atcccgcttg cttctaaaat gatttccggt actcttacga tatcttttct
    12121 caattccgtc atgatttctg gcgtaaattg ttctgccatc agccacttct cctttgctat
    12181 ctccggtcat actgattcaa ttgatacgtc tcgatgacct ggatgatttt ttgcgcataa
    12241 ccggggtctg tcgcataacc tgcatcttgc aatgcttgtg cggcttcctg atagttggtg
    12301 gccgtaatca ctttttcata cagcgcagga ttccaatcta ctccttggac gaacagttgt
    12361 gtatggtcat ccatggactg ttcccatgaa tcatacactt tgaagtctcc ttggatagtt
    12421 atccattctt cgttaacaaa ttcctttgtt tccaggctta ctttcttctg atccccataa
    12481 gccttgatcc cgaaaagatt attgtatttg ctagccaagg tactttttcc ccaatttgat
    12541 tctaatatag cttgtcctaa aatgatacta ggaagcactc cataaccatc ttgtagttct
    12601 tgggcatgcg gaacaagccg ttcaataaat tgctgatggg attgcgcttc attttcttgc
    12661 tgattatcag taaaaggaga agacagattc ttcaaggaaa aaacaaaggc taagagtatc
    12721 actgctacac cggcaaccac tgctggccaa cggatattct tgcgcttttg ctttttccac
    12781 ttttttttcg ccatgatcaa ttcctacttt tcatttaatt cattcagata ttcttctagc
    12841 gtcaattggt gtttggtgat atactcagcg ttttctttac cgacataacg taagtgccaa
    12901 ggttcatatg tgatcttagt tatatcttcg tgtccttttg gataacgaac gataaatcca
    12961 tactgcgcag cattatctgc gatccattta gctccaggct gatctccgta acttgcatcc
    13021 aatacagtag aaggataact gttgtaccag ttttgatcga cgacatcgac tgccagacct
    13081 gtgtggtgtt cgctgtatcc tggctccgta atcgtttctt tagtaatctt tgtcgcctcc
    13141 tcttctgata caccttgacg actcatcaca tcttggacat tttgttcgaa aacttgttgt
    13201 tgggatgata cagaccggta agctgagacc atcactaatg gaaatccagc tgcttcagca
    13261 gccttggcaa atgcttcata ttctggagca atgcgtttgt cgatcaagta tccgttactt
    13321 aatgatgcca attgagtaga ctcatcaatt tctttttcta atttgtgatc tggaccaacc
    13381 aaaactagct tccaatcgtc tgttttaaca tctggcaatt gtttggacgt acgatctttt
    13441 gtttctttgt cctttacctt gctgctggtt gtttgactgt tcttttcttt actagcagtt
    13501 gttttggtgc ttgttcgttc gactgcttga ttgccggctt gaacatgcga atcatcatcg
    13561 ctaaaaataa catagcccat tgctgcgatc ataatcacaa ctgctccaaa tcctaaaaat
    13621 ttattcactt gaaaaatcct ttcgttgttc ttcacgttcc ccattcgcct catgaacgta
    13681 agtttcttcc agattttcat tcacccaatc gagaagttcg attttttctc cttcgatctg
    13741 tttttgaaca tctgctggca gacgaaaatt caaaatatca tcatagcccc attcatcata
    13801 ttgaatactt actttcaagt caaggtatct accagcctgc tgattatttt tcaaagggat
    13861 cagctcgact tttgctgctt tgctgctaag ccatttttgc gcgatgcgtg ttttgatttg
    13921 cttgtactct gcaacggcaa cgtttcgttt ttcgggaaaa ataatcgttt gacgcaaaat
    13981 cttttgcgtt agcatccatt cataatcagt gaaaagattt tttactttct gcatcgcttc
    14041 agatggcaga tttttctcgc catctttcca tttttgccat tgttctggac tgatcagcaa
    14101 gtattcatct aaaaaatttt tttcagtttc aaactgttct aataatacac tcataataag
    14161 atcaatatat acttgatgca cgtttatccc ctccttttaa ctattctact taagttttga
    14221 tcaaaaagca cctcttaaat cgctgctttc atttttttta atgtttcaag tcctctcctc
    14281 tttattcttc gtgatttttg ttataataat agacagacaa gaaactcaat ccaactatga
    14341 aggagcgttt tcagatgaaa aaatatcaat ggggaatcgt tggtcttggt accatcgcac
    14401 atgagtttgc cgaaagcttc aatcaagaga cgagcgaact aacagcagtt gcttccagaa
    14461 cttcagaaaa agcggaaaac ttcgctcacc gttacaatat tccaaaagct tacggaagct
    14521 atcaagaaat gttagacgat gctgaaatcg acatcgtgta cattgctgta ccaaatagac
    14581 agcatatcga ccatatcttg gcagcattgg aagcaggcaa acacgttcta tgtgaaaaag
    14641 ccatcacgat gaataaaaaa gaactggctg acgctatgcg cttagcagaa gaaaagaatt
    14701 tgatcttagc agaagcgatg acaatcttca acatgccact ttatcagcag ttacgctcaa
    14761 taatggatac aggaaaactg ggggctttaa aaatgatcca agcacctttc ggcagttaca
    14821 aagaaccaga ccctaaaaac cggttcttca atccagaatt agctggagga gctctgttgg
    14881 atatcggtac gtatgctgta tctttcgctc gtttcttttt gagctctcaa cctgaagtag
    14941 ttgcctctac aatggttcct tttgaaacag gcgtggacga acaatccgtc actatcttgc
    15001 gtaacaaaga aaatgaactt gctgctgtca gcttgacttt tcaggcaaaa atgccaaaag
    15061 tcggtatcgt tgcctttgag aacggttatg tgacgattgc tgattacccg cgtgctgatc
    15121 gtgcggagat cttatttaca gatggtacaa aggaattcat cgaaagcggg aatacatctg
    15181 aagcaatgaa ttacgaaata aagaatatgg tacacatgat tgaaggtgac ttgcccaatc
    15241 gctctctctt cttgacaaaa gatgtcatcg aaatcttgga tcaaatgcaa gttctatgga
    15301 aaaatatgta attttaaaag gttgtgaaaa agctgttctg catcaagtaa taagaacagc
    15361 caaacaaaaa tagctcctcc tatttttcgt ttggctggac ttattaccgc agaggaaagc
    15421 ttttgaacac cgtttaagaa agaggttgtg agcctaccgt tcagctccga gtcacaagga
    15481 gcaatttcaa aaaacagctt ctcatgtttg ttgaatattg tgacttgtga ccgaggagtt
    15541 gacaggagaa caccgtttaa agaagaggtt gtgaaatcag ctttttgacc tgaatattag
    15601 ctgaattcac aacctctatt tttggatttt ttgttgggag aattctgcct tgatttcaat
    15661 aaaactttta aaagactgca aaaactggaa caattctgct cgatcctcga attcagggcg
    15721 actagtcggc agaggcattt gccgatacaa tacataaact tcttcaatac gtgttaaaat
    15781 ttgtctgcca tcatttgact ctgaaaatgt ttctgcagta taaatcaata aaccatagat
    15841 atgttttcca taataagctg gttgctggat tctctctaga ttttccagca tgtctttaat
    15901 cacattggcc tgtgctcttc tcatagagaa gtaagtttcg tagtatagcg gctgtctgag
    15961 ccagtagttt tcttgatgtt ctctggcatc ttcttggctt tcgcgaatga atgtcagtaa
    16021 atgttcgcat ttgatctgca catcttcttt attttctgat aaaagaaatt ctgccatttg
    16081 tctcaaaata tttcgaaatt ctttttcgat ctgtatttgg ttttccttca gctgcttcgt
    16141 acgatctggc atgtaaacat ttgcaagcaa agcaaatact aatccaatcg ccattaatcc
    16201 cgcttcattg ccgatcagct gccaagacat gttttcctcc actaaataat gagtgaccaa
    16261 aaccgaattt actactatcc cttcggttaa tttgaagcga gaggcaagcg ggatgaacag
    16321 gaataaaaaa agactgaagc caaaaatagt atacccaaca gcaaaaaaac tgaacagtgc
    16381 tgttgccgta gctaatataa aggctgcgat tcgattcatg cccgtacgca atgtagagcg
    16441 cttggtattt cctacactta ataatgcaat gatccctgca gctggccaat acaacagcga
    16501 taagctaccc gctgtgacca tcgaaaagaa gacactgatc actgttttga ctgttcgcat
    16561 tcctatacgc atttacttcc ccctttttta ttttaattat acttgaataa tgaaaaacta
    16621 gctatcagac aaaagtttag gtatgatagt aaaaagacca aaaaggagaa tctctatgca
    16681 atttggccgt tttcgactag gtatgcgtac gatcaaaagt gccttagctg tttttttatg
    16741 tatcttgttt tttcatttga ctcaacgagg aatcccgatg attgctgcat tatctgctgt
    16801 tttttctttg cgtcaggact tgactacaac cgtttctttt ggaaagtcta ggatcatcgg
    16861 caatacatta ggtggtacat tagctatcat ttattttttt gtcaaaagct attttcacaa
    16921 tgattttttg gtagaattga tcttgcttcc tattctagtt attattgtga tcgtcgtgtc
    16981 agatggtatt aacaataatt ccggtatcat ttccgcaata gctacgttgt tgctgattgc
    17041 gttgagcatc cctcaaggcg aatccgcttt gtatgctatc caacgtgtac tagatacttt
    17101 tatagggaca ttcattgcaa tcggtatcaa tttctttctt cgtcctcctg aaacagaaaa
    17161 gaaggaagaa ctatcagaag atttggtcga actccaaaag aaagaacgat tgctaagaac
    17221 aaatttacaa gagatagaag ctaagatcca aaaacaaaag aaaaatagcg aggactaatt
    17281 ttcttttgca ataaaataaa aaaatggaga gaagaacttt aaaaagtctt cctccatttt
    17341 tgattgaacg gtgaagtgta ctactttatt cttccttcat cgagttttgt tctgcctctt
    17401 ttggcgcttc atacatattt acttggctag tagtcccacg tgtcgacaaa ttctctacga
    17461 tttccttcaa gtcaagtcct gttgtcgctt ttagtgtttc ttgagtagaa gaaagcaaat
    17521 ttgtggcata atttgtcact cggttcgctc cactggtttc tcctgaactg cttcctgtat
    17581 cgacaacaga gattttttcg atatttccta gcggctgtgc tgcttcgcgc atcaattgcg
    17641 gtagcatatc gataaccata ctcaagacag ctgcttcgcc atattccttg aaggcattgg
    17701 cgatcttttg ttttgcttcg gcttctgctt ttccttttgc tagtgctgct tcggcttctg
    17761 cttgtccagc taaacgtgtc ttattggctt ccgcttctgc cagtgcttcc acacggaatc
    17821 gttctgcttc ggcttcagtc acttctttga ctttttgagc ttgtgcttct tgttctttag
    17881 cgtaccgatc ggcatctgct tttttcttca cttccgaatc atattgcttt tcacgacgtg
    17941 tgatttcttt ttcttccagt tcgatttgtt tttggcgttc tacgatttta atctgcattt
    18001 cctgttcgat tacttgttgt tgtgctcggg cactttctaa attgtaggct tggtctgctt
    18061 ttgctttagc aacatcttgt tcttgtttgt aggttgctaa tttcagttct ttttctttta
    18121 atgattctgc gatctctgtt tgtcgctgta attcagctgc ttgtgattct ttttctgctt
    18181 gagctttttt gatacgcgtt tcttttagag cttccgcttc tgctatatct gcatctcttt
    18241 tgacttgggc gattcttggt ttccctaaag agtcaagata accgtttttg tctctgacct
    18301 ctttgatagt gaaagaaacg atgatcaacc ccatttttgc aaggtctaca gaagcgactt
    18361 cttgtacgct ttgactgaac ttatcgcgat tttgatagat ttcttctaca gtcatagaac
    18421 caagaatcga gcgcaagtgt ccttctaata cttctcttgc ttcattttcc agttcttctc
    18481 ttgtcttgcc taaaaactgt tctgctgctg tagcaatctc ttctacagat gaaccgattt
    18541 tgatgatcga agtaccatca cacatcaccg gtacgccttg ttccgtatac acttcagggg
    18601 tggagacgtc caatttactc gataatagac tgatccgatt cgaacgttga aaaactggca
    18661 agacgaatgc gccacctcca cgcacaattt tgattttgtt attgctttca tcaatgtgca
    18721 catttttgct tcctaaataa cttccactga tgatcaacgc ttcatccggt tttgctgtct
    18781 ggtacttcgc tacaaagacg atcagcaaca tcagtaaaat caaagctgcg attgcgatcg
    18841 cgataaaaac aactggtatt tccatttaac acacgctcct ttaagctttt atcatgcttt
    18901 cataaggcat gacataacag attctttcct tgatctcaat gatcaatact ttcgttcccc
    18961 gctcagctgt ctgttcttgg gggctgtaaa aacaagctgg acgactaatg gaaccggtaa
    19021 cacttttcaa ctgaatttct cccatacctc gtacagggat cggtgtgatc acaacagcta
    19081 cttgtccttc cagagttttt tcagaagacg acatcgttga ttcggcatgt ttcatcggca
    19141 tcaatatata gaaattaagc aaaaatacca acactatcga tatgcctcca ctagcaataa
    19201 ataaaaataa agatgattga tctgttaaac gctcacctag aaaaccaaat aagcttgtga
    19261 aagttaacca agggataata agcatcggat caagcgggcc atcaaaatcg aatacatctc
    19321 caaaaataat caataggact gccaatcctg cacaaatcag caatgtatac caataaatcg
    19381 tctcaagtgc gattccccct atcatataca cgcctccttc catttctacg tctatcatac
    19441 caatgtttga aaattctgta tagcttcttt taccgactca gtggtgtttt ttataggaag
    19501 gttgtgttta tcgttgacaa tttgttaaat ttttgttttc atgttaaaat tttcacaata
    19561 aattctttcg aggtagccta tgtttccttt ttttaagaaa aaaccattaa agctccagaa
    19621 cacgatcatc cttgtcgttt gtttctccac attcgtttct ttgattgttt ctgccatatt
    19681 gatcaggaat tttattcttt cgatgaaccg atctagtgtg tcagataaaa tctctagcat
    19741 tgctaaaatc gttgccaatg ataaactcat ccaaaaaaag ctgcagactg gtgacccgac
    19801 acatgcgatt caaggaataa caaatgaaat catgattgca tcgaatttag attttatcgt
    19861 tgtcatggac cctgaaggca ttcgatattc tcacgcagac ccaagttaca tcggtcttcc
    19921 tttctcaagt catgaagatg tgaaaaaagt gattggagga caagaatttt tctctgaaaa
    19981 agaaggaatg ttagggttcg gcttccgcta tttcgtccct atttattatg atcaagagat
    20041 tattggtgca gtttgtgttg gcttaacaaa caacacgatt gacaatcaag tcaaagatgg
    20101 acaaaaaatg attgtttttt cattgattat tggacttagc gtaggcatca taggcgccgt
    20161 ttttcttgcc caaaaaatga aaaaagtact gcttggactt gaaccacaag aaatagcaga
    20221 acagttaagt gaaaaaacga ttatcgaaaa tgaagtagat gaaggaatct tagccatctc
    20281 ttctgataaa actatcctct tgatcaatac acctgcaaaa aacattttct accaaacaga
    20341 gcagctattg gatttcgaag taggaaaaaa atcagtgata atctatatga cgttttgttt
    20401 aaagagacga ttgagcatca aacgaccgtt cggactcaat ccatgtattt aaatgggata
    20461 gaaactattt ctagcgttac acctcttttt agcaatgacc gttttttagg agcagtcgca
    20521 actttacgaa atcgatcaga agtaatgaaa ttaatggagg agcttggggg gacgaaacaa
    20581 tatataaact ccatgagagc tcaaacccat gaattcatga ataaacttca tgtcatttct
    20641 ggtttgattg atttgaagaa gtatgaagaa gtcacccatt ttatccagca gctaaatcaa
    20701 aattataaaa aagagattgg ccaagtctca agtctgatca aagtacctgc aattgccggt
    20761 tttttattag gcaaaataaa cgaagccaaa gaacaatctg ttgaaatgct gttagaaagc
    20821 gattctttta tccctaaaat ggagatggat gactccatcc attcactttt gcaagtgtta
    20881 gggaacttac ttgataatgc aaaagaagcc gtgctacaaa aacatgaaag aaacggacag
    20941 gtccggattc atttaaacta tgacacagaa gctgaagtat tcatcgtgac cgtctgcgat
    21001 gacggtgtcg ggatatcaga agaaatctca gaaaaaatat ttacgctagg tttttcgacg
    21061 aagggaatca atcgtgggtt tggattgaat ctcatccatt caatcgtcaa acgccacggt
    21121 ggatttttag aattttccaa acaaacaaat ggcggtacaa tcgtctatct agaattacca
    21181 aaaaaggaag atcaaaatga tgacaaatgt cctgataatc gaagatgatc caatggttgc
    21241 agaattgaat cgtacttttg tggaaagcat ccctaatatg aacgtaatcg aaagtttcag
    21301 aaatgtagaa gaaggaaaaa aatatttcag acaaaaaaac gcagatctgc tgctgcttga
    21361 tgtatacatg cctggcgaaa gtggtatcca ttttttatct tggcttagac agcaaggaaa
    21421 tgatatccca gtgattttga tcactgctgc agatgatatc gaaaccataa agaatgtgat
    21481 ccgctacggt gccttagatt atttgatcaa accgttcact ttgcaacgtt ttcagctggc
    21541 gatcgaaaag tatcttgctt tctctcagac aatccaaacg actgaaaaag ccgatcagaa
    21601 attgatcgat caagtattca accacaccac tcccattcac gaaataccat cattcatcga
    21661 tttgcctaaa ggtctgtcaa aactgacttt aaaaaaaata agtgcattta ttctaaaaga
    21721 agatactatt ttttcaacaa aagaactggc tcaaaaaagc tcactgtcac gtatatccgt
    21781 caagaaatat ttgaattttt tagtagaaca agatatattg aacgaaagaa tcaaatattt
    21841 ggaaatcggc cggccgctaa cctgttatga attgacaaag aatgggcgga aaaagctcac
    21901 agattactgt tgagtcatcg aattatccca tacaaaatag tccaagtata cagaagtaac
    21961 aactgtttac ttggactatt tttaaacatt ttattgacga atatccatgt tttcttatct
    22021 taattgacgg gctttaggaa gagatacctc atttttcgtt tttccagttt ctattatttc
    22081 acgaaagttc tcgaaacttg tcgaaatcat atttgataat gcctcatctg tatttgaacc
    22141 tacatgcggg gttacaagaa ctctagggta catttctact aattgctgga tagcagggtc
    22201 tggaatcgtc tcccatggct tgaaggatcg gaaaaaaacc tctgtttcat ttgcaaaaac
    22261 atcggttgca aaaccagcta aatgattgct tttcaaagct cttaaaatag cttcattatc
    22321 ttgaagctct ccacgggctg tattaatcaa gatgctacct tttttcattt gagctaaaaa
    22381 tccttcattg atcatttgat cgttttcacc tgggatatga ggtacatgaa tactgacaat
    22441 atcgcttttg gctaataact cttcaagcga gtggaacgtc aatacttttt tagcagcttc
    22501 tgtctgatga atatcgtagc cgatcacttt ggctcctagt cccttaaaca aaacagcttc
    22561 cgtcaaccct atcctgcctg ttcctatgat ccctactgtg caatttctga tttcacgact
    22621 aaacatttgc tcatccactc gaaaatcttt gaaagatgtt cggatcgtcg tatacgcagt
    22681 atttctcaaa agcatcatgg caaaagtcaa agataactct gctatcgcat ttggcgaata
    22741 cgatggtaca cgggctactg tcataccaaa atcagctgcc gcttgtagat cgatatgatt
    22801 gactcccaca gtccgtgtaa aaacataacg aatcccatat tcctgcattt ttgcaagatt
    22861 ggtctcatct gctatacaat ttcccctcaa caatacagca tcatggtcct ttgctgacac
    22921 gatattgtca tgtgtcaaca gttcttcata aagactgaga tcataatggt aaatattcaa
    22981 tttttcaaaa aaatctatct cgttcggacg aacaccgtaa catgcgattc tcatactttc
    23041 ttcctccatt tcttatgaaa ataaacctat gccatgaatc atttgtaata caacgatcca
    23101 tacaggtgtc agtaccacag caaaaatagt tgacagtaag gatgcattgg aggctaaaag
    23161 tgcctcccta tcaaaactga tagcataggc tgctgccact gttgcagctg gtgtagccat
    23221 catgataacg attgttgcca gtgcttcaaa attgactggt agaatattcg ttgccgtcaa
    23281 aactgctaga atcacgatat tcaatgcagg tacaaatacc actttgacta gactgtaata
    23341 ccatgaagtt ttttcagatg cagctttttt gaagcttact tcacctaatg ttgcgccgat
    23401 tgctagccac gccaatggtg aacttaaacc agctaaataa tccatcggct gaaatagcca
    23461 cggtgcagtc tggtcaattc gcaaaaacgc tacttggctt atctctcctt ctgcacctgt
    23521 aactgacact tgtggcagat acccttggaa cagccaaatg aaaaggccgg caaaagtagc
    23581 gataatgatt ggattgacga acatcgcttt gatattttta gcttccatct tcaagccact
    23641 catttttatg tatccataag aatacaaaaa gattcggtag ccaatattga aaattgagga
    23701 atacatcacg ccgacaggac cataaatcgc actgacgata ggcgttccga aaaaggtcgt
    23761 agaaccaaag atcgtcagga cacgcaacgt tgtttgctta tcccctttat agcttaagaa
    23821 aaaaggtttt gaaactagaa taagaataat ataaataact aatccccaaa tcaagacatt
    23881 gattccttgc atcagtaatt ctgtattgat atcttgcatg aaagctcgaa atgccagtgc
    23941 tggcaaagcc actgttaaga cgactttcga tagaattttt ccaacagatt cagaaaaaat
    24001 cccctttttt ctacaaacaa accctaataa tataataaaa actgttgaag tgattgcact
    24061 gacaatcgac atatctgtca aagtcgatcg aaaaatgtca aataagttca tctttctcct
    24121 cctaattatg ttcatttatt cacaataaaa aaagctttta ttttgctaat acgtattaac
    24181 atttttaatt ttatctttac attgttactt attgaacaat aggaaattaa agaaactttt
    24241 gaaacttatg taaagtgatt tttactagaa cacaaaaaaa accttaaaac caaacttaag
    24301 aagtttagtt ttaaggttaa cttaaattat attatgcgtg gaaacgttcc acgccatcta
    24361 gataagttgc ttccaatgtc atatcagggt tcaatacgat aaagtctgca tcgcgtcctt
    24421 gagcgatttt tccacatgta tcatcgattt tacagctgat tgctggaatc agactagcca
    24481 tcatgactgc ttgttctggt gttgctaatc cccagtctac aacattttta attgcttctt
    24541 tcaatttcaa tatacttcca gctaagttgc cagattctag acgtgctgtt ccttctttta
    24601 ctactactgg aaattcgcct aaattgtaat ttccatcagg catcccaccg gccatcatac
    24661 agtctgtaat taatgcgaca tgatcatgtc ctgctttttc cattaggatt cccgcagcat
    24721 ttggatgtac atgatggcca tcacaaatca attcagaaaa gacatgttct aaagataata
    24781 acgcacccac catacctggt tcgcgatgat tcaatccacg cataccattg aaagcatgga
    24841 caaatacact tgcgcctgct tcaacagctt tttgcgcttc ttctagtgta gcattactgt
    24901 gtcctagaga aacaactact ccttcttcag tcacttgttt gacaaactct tctacacctt
    24961 gacgttccgg tgctaaagca atttttttga ttaaaccacc tgaagcttct tgccattcat
    25021 ggaaagtatc caaatcagga tccccgaaat aacttgggtt ttgtgcccct ttgtgttctt
    25081 ctgtgaagaa aggaccttca aaatagattc ctttgatttt agctcctggt gcttcctgat
    25141 acattttgcc gattgtctca gctacatctt ttaaacgttc tttgcttgac gtcaatgttg
    25201 ttggtaaaaa tgaagtcact ccacaagata acagaccttc agacatcact ttgatgcctt
    25261 ctgcatcatt atccattaca tcatgattca tatagccgtg aatatgtgta tctaccaaac
    25321 caggagcaat ccatttgccg ctttggtcaa tgatgttgac ttctccctca ggtagttctt
    25381 tggtgtattc accaaaaagt ccatcggtaa tttcaagata gcctgcatgt ttgacacctg
    25441 acgctaaaaa gaatttgtca gcatagataa aagttctcat tctattctct ccctttgttt
    25501 ttctacctct aaagtactct tatttagaga agaagtcaag aatggtctac accatttaca
    25561 aattttttga aatttgtttc acagcttgtg ctaagccttc atagaatgct gctctttctg
    25621 gttcttcttt acgcttgtat attaagcttt taagcacaaa taaatcattg aggtaatagt
    25681 agctattctt ctcttgagcc caatagattc cttgattcac ctgttctaac gcttcgtcga
    25741 tttcattttg atggaataag aaatttccat aattataaaa aatttgcgaa aattcacttt
    25801 ttgatcgttc atcagctgat tgatgcagca gttggatgct tcgactcaaa tagtagcgtg
    25861 cttcagccgt ttttcctgcc acaccatata tttttccgat acaactaatc agttgtattt
    25921 ctgtgtcaga aacatggatt ctttcttttt ggctagagta gcttaatgct tctttcaaat
    25981 aataaaggga ctgagagata ttttgacgca acgtgaattc gcaaacaccg agataataat
    26041 aatagcgttg aaagtcagtt gcttgataca gattttcaga aatatcagaa gattttagta
    26101 cttgttctaa cttccgataa tcacgttttt ggaaacaact atccaacagt tcaaataatt
    26161 tatcgtttgt ccgagtagtt tttcgagaga gattcatcac atgatcaatc gttaccccta
    26221 gccgttgaca aatctgcatc atcaccaata cattcggcag ttcctcatta ttttcgatcc
    26281 gacttaatac actttgtgaa caaatatcat gcgaaagcat tttttgcgtc attttttgtt
    26341 cttttcgaat ctctttaatg atcgttccaa aggactcgat taacgtttcc ataagtatcc
    26401 tccccaaaat atgcgtattc gcattctatt cgtattttac tttaggaagt cgcaaacttc
    26461 aagaaaagac ttgcaactaa ataatatact ttagttattt aagaaaactc cactagaaag
    26521 agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa atttggtttt taaaatcatt
    26581 tttggcgata ggttgctagg aattctctca ttttcttagc tgtttgtttc aaatcatcca
    26641 ttttaggaag ataaacgatt cgaaaatgat ctggctggtt ccaattaaat ccaccaccat
    26701 gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac gaatttttca tcatccaaaa
    26761 tattgaatcg ctttgtatca atttttggga atatataaaa agcagctttt ggttttacgg
    26821 ctgaaagacc aggaatatca ttgattgcat tatagatata ttctctttgt tcatagatcc
    26881 ttccgcctgg caggagcagc tcatcgacac tttgataacc gcctagcgcg gtttggataa
    26941 tttgctgaga caatacattg gaacacaaac gcatggagga caacatattc agcccttcta
    27001 tatagccttt gacgtgtgac ttatctccac ttaggaccat ccagcctact cgaaatcctg
    27061 ctacacgatg agatttggat agaccgttca gcgtaacaac gaacaaatca ggcgctaatg
    27121 tagcaatcgg aatatgttct agaccatcca tcactaagcg atcatagatt tcgtcggaat
    27181 aaataatcag gttattttgt cgagctactt caacgatttc ttctaataat tcttttggat
    27241 aaagtgcccc agtaggatta ttaggattaa tgataacaat agcttttgtc cgactcgtta
    27301 tttttgattt gatatcatca atatcaggat accattctgc ctcttcatcg caaatataat
    27361 gaacaggtgt gcctccagct aatgcaatag aagccgtcca caacggatag tccggcattg
    27421 gaaccagtac ttcatctcca ttgtccagca acccctgcat acacatcgtg atcaattcgc
    27481 tcactccgtt gccggtgtag atatcattga tcgtcacatt cggaaaatgt ttcagttggc
    27541 aatattgctc aattgctttt ctagcagaaa agatcccctt ggaatctgag tagccttctg
    27601 agtcacgggc attcatgatc aaatcacgaa tgacttcgtt tggtgcttca aatccaaaag
    27661 gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc ctcttcttgc atccgctccg
    27721 cttcttctag aacgggaccg cgaacatcgt aactgacacc ttctaatttg ttggattttt
    27781 caaattctct catcacttag ccaatccctc atttcaaaaa gttagaactt tccaaatttt
    27841 ttgttattta ctatttcaat atcatactcc atcctagaaa attttgccac ttatttagga
    27901 aaaacgtaag tattaaaaaa acacaaacaa gcgttgagaa cttaattgtc atgatagtca
    27961 ttccatagat tcgctgccca atccattgca attccacaaa tgattcctag cataaaacca
    28021 agcaaaaaac gtccgaatgc aagtccaatc aatacaccta agatcattcc cagtcctgaa
    28081 aattctccgt catatttctc catcttaata agtcctccca tttttgagct tgacaactat
    28141 gtaaaacctc gctatcatta gtgtgttatt attacgcaga tgtgttggaa ttggcagaca
    28201 agcatgattg agggtcatgt gagcgttagc tcgtgtgggt tcgagtccca tcatctgcac
    28261 tacaactcgt gaagttaatt ttgcttcacg agtttttttg tttgaacgtt tctttatgcc
    28321 attatataca tatcactgta tcctttacaa aaagaacgta caatcatgta aacagcctga
    28381 ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa cttgcaagtg ttaaaacaat
    28441 taatcgaaaa acgagtaaaa tccctcacct aatactaaag cgagggattt tttgtatatt
    28501 atctgtttat caccgttatt ataaaggtac aaataccaag cacggttaat ccaaattgca
    28561 taaattcatt tttttgtaca ctcatgcttt tcattagctc tttaactgcc attagtacta
    28621 agatggcgat aacaacataa agtatatagt acgtccgcat tccggaagcc catactgtgg
    28681 gagaaaatcc cataattaac cttgaacaaa atcccattat catcaaaata actaaaaaaa
    28741 tagaagtttt cttattgtca aaacttaaat aaattccaac aattaaacat agtaataatg
    28801 ccaagactac taaagtagct atccaagtac ctggataaaa aagtgacagt ttcgttccta
    28861 actctgtaaa gagattattc aggttactac tactccaaat cattgccctt ttatttccat
    28921 tgacgtaagt aaagctttga cccattgtat taccaaaata aataatcagg tttaagaaaa
    28981 acggtaatgc agtcaatata cgcacataat agttttgaca tttcctatat gttagaagga
    29041 aaattaagaa aaatagcaat aaaaataaga tattcatgtc taaaaaaaac ggctttccaa
    29101 atgatgaaat acctaaatct aacttattaa gaaagttgaa atttttatat tcaggaaacc
    29161 atttattaat ttcatgtgca gcacgtaatg cattccctgg agttgtcaaa gagaaaatta
    29221 attctgctaa gcttattatt gaaaatactg ataacttata attatatcgt ccattgacaa
    29281 tcaaatataa actaactata cttgttagaa caaaaaaaca ggcgttgact tgttcttgat
    29341 tagttaaaaa tatcaataaa ggaatactta cccaatatat ttttctattt atttcttctc
    29401 ctcttaagag ttgaaaaaac ggataaaaag ttagtaaact aaatgctact ggccactgat
    29461 agttcaaggt ggttgctctc caaccggttt cccccattac tgtcaatggt attaaacagt
    29521 atatagagca aaccaataat aaatttttaa catttatttt ctcaaaaaca ta
//
LOCUS       DACDYJ010000031        29109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDYJ010000031 DACDYJ010000000
VERSION     DACDYJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.031"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1030)
                     /locus_tag="IWM51_001427"
     CDS             complement(<1..1030)
                     /locus_tag="IWM51_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9970853.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="IWM51_001428"
     CDS             complement(1112..1801)
                     /locus_tag="IWM51_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9970854.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IWM51_001429"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IWM51_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP9970855.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="IWM51_001430"
     CDS             complement(2328..2831)
                     /locus_tag="IWM51_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP9970856.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IWM51_001431"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IWM51_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP9970857.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IWM51_001432"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IWM51_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP9970858.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="IWM51_001433"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="IWM51_001433"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP9970859.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="IWM51_001434"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="IWM51_001434"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP9970860.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="IWM51_001435"
     CDS             complement(6443..7462)
                     /locus_tag="IWM51_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9970861.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8522)
                     /locus_tag="IWM51_001436"
     CDS             complement(7635..8522)
                     /locus_tag="IWM51_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9970862.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IWM51_001437"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IWM51_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP9970863.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IWM51_001438"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IWM51_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP9970864.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IWM51_001439"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IWM51_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9970865.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="IWM51_001440"
     CDS             9753..10397
                     /locus_tag="IWM51_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9970866.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="IWM51_001441"
     CDS             complement(10453..10956)
                     /locus_tag="IWM51_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9970867.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="IWM51_001442"
     CDS             11553..12518
                     /locus_tag="IWM51_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9970868.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="IWM51_001443"
     CDS             complement(12617..13789)
                     /locus_tag="IWM51_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9970869.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="IWM51_001444"
     CDS             complement(13776..14921)
                     /locus_tag="IWM51_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9970870.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="IWM51_001445"
     CDS             complement(14935..15867)
                     /locus_tag="IWM51_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9970871.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="IWM51_001446"
     CDS             15984..17063
                     /locus_tag="IWM51_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9970872.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="IWM51_001447"
     CDS             17060..17689
                     /locus_tag="IWM51_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9970873.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="IWM51_001448"
     CDS             complement(17799..18026)
                     /locus_tag="IWM51_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9970874.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="IWM51_001449"
     CDS             complement(18129..20717)
                     /locus_tag="IWM51_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP9970875.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="IWM51_001450"
     CDS             complement(20737..21396)
                     /locus_tag="IWM51_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9970876.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IWM51_001451"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IWM51_001451"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP9970877.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="IWM51_001452"
     CDS             complement(22169..23035)
                     /locus_tag="IWM51_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9970878.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="IWM51_001453"
     CDS             23144..23785
                     /locus_tag="IWM51_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP9970879.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IWM51_001454"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IWM51_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP9970880.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="IWM51_001455"
     CDS             complement(25226..26125)
                     /locus_tag="IWM51_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9970881.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="IWM51_001456"
     CDS             complement(26127..26924)
                     /locus_tag="IWM51_001456"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP9970882.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="IWM51_001457"
     CDS             complement(26902..27606)
                     /locus_tag="IWM51_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP9970883.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="IWM51_001458"
     CDS             27737..28312
                     /locus_tag="IWM51_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP9970884.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..29107
                     /locus_tag="IWM51_001459"
     CDS             28430..29107
                     /locus_tag="IWM51_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9970885.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8565 a         6169 c         5232 g         9143 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taattaaaa
//
LOCUS       DACDYJ010000032        28297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDYJ010000032 DACDYJ010000000
VERSION     DACDYJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.032"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(212..907)
                     /locus_tag="IWM51_001460"
     CDS             complement(212..907)
                     /locus_tag="IWM51_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP9970886.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(997..2097)
                     /gene="ychF"
                     /locus_tag="IWM51_001461"
     CDS             complement(997..2097)
                     /gene="ychF"
                     /locus_tag="IWM51_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP9970887.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2106..2297)
                     /locus_tag="IWM51_001462"
     CDS             complement(2106..2297)
                     /locus_tag="IWM51_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP9970888.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2318..3208)
                     /locus_tag="IWM51_001463"
     CDS             complement(2318..3208)
                     /locus_tag="IWM51_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP9970889.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3195..3962)
                     /locus_tag="IWM51_001464"
     CDS             complement(3195..3962)
                     /locus_tag="IWM51_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9970890.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3974..4687)
                     /gene="rsmG"
                     /locus_tag="IWM51_001465"
     CDS             complement(3974..4687)
                     /gene="rsmG"
                     /locus_tag="IWM51_001465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP9970891.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4919..6820)
                     /gene="mnmG"
                     /locus_tag="IWM51_001466"
     CDS             complement(4919..6820)
                     /gene="mnmG"
                     /locus_tag="IWM51_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP9970892.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEVQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6837..8234)
                     /gene="mnmE"
                     /locus_tag="IWM51_001467"
     CDS             complement(6837..8234)
                     /gene="mnmE"
                     /locus_tag="IWM51_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP9970893.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8578..8916
                     /locus_tag="IWM51_001468"
     CDS             8578..8916
                     /locus_tag="IWM51_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970894.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(9084..9842)
                     /locus_tag="IWM51_001469"
     CDS             complement(9084..9842)
                     /locus_tag="IWM51_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9970895.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9860..10678)
                     /locus_tag="IWM51_001470"
     CDS             complement(9860..10678)
                     /locus_tag="IWM51_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9970896.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10675..11031)
                     /gene="rnpA"
                     /locus_tag="IWM51_001471"
     CDS             complement(10675..11031)
                     /gene="rnpA"
                     /locus_tag="IWM51_001471"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP9970897.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11149..11283)
                     /gene="rpmH"
                     /locus_tag="IWM51_001472"
     CDS             complement(11149..11283)
                     /gene="rpmH"
                     /locus_tag="IWM51_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP9970898.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11807..13141
                     /gene="dnaA"
                     /locus_tag="IWM51_001473"
     CDS             11807..13141
                     /gene="dnaA"
                     /locus_tag="IWM51_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP9970899.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13339..14469
                     /gene="dnaN"
                     /locus_tag="IWM51_001474"
     CDS             13339..14469
                     /gene="dnaN"
                     /locus_tag="IWM51_001474"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP9970900.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14698..14940
                     /gene="yaaA"
                     /locus_tag="IWM51_001475"
     CDS             14698..14940
                     /gene="yaaA"
                     /locus_tag="IWM51_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP9970901.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14927..16051
                     /gene="recF"
                     /locus_tag="IWM51_001476"
     CDS             14927..16051
                     /gene="recF"
                     /locus_tag="IWM51_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP9970902.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            16048..17994
                     /gene="gyrB"
                     /locus_tag="IWM51_001477"
     CDS             16048..17994
                     /gene="gyrB"
                     /locus_tag="IWM51_001477"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP9970903.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            18014..20485
                     /gene="gyrA"
                     /locus_tag="IWM51_001478"
     CDS             18014..20485
                     /gene="gyrA"
                     /locus_tag="IWM51_001478"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP9970904.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20721..21020
                     /locus_tag="IWM51_001479"
     CDS             20721..21020
                     /locus_tag="IWM51_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP9970905.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            21070..21582
                     /gene="ssb"
                     /locus_tag="IWM51_001480"
     CDS             21070..21582
                     /gene="ssb"
                     /locus_tag="IWM51_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9970906.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21608..21844
                     /gene="rpsR"
                     /locus_tag="IWM51_001481"
     CDS             21608..21844
                     /gene="rpsR"
                     /locus_tag="IWM51_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP9970907.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            21987..23960
                     /locus_tag="IWM51_001482"
     CDS             21987..23960
                     /locus_tag="IWM51_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP9970908.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            23966..24418
                     /gene="rplI"
                     /locus_tag="IWM51_001483"
     CDS             23966..24418
                     /gene="rplI"
                     /locus_tag="IWM51_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP9970909.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24692..26059
                     /gene="dnaB"
                     /locus_tag="IWM51_001484"
     CDS             24692..26059
                     /gene="dnaB"
                     /locus_tag="IWM51_001484"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP9970910.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            26839..28131
                     /locus_tag="IWM51_001485"
     CDS             26839..28131
                     /locus_tag="IWM51_001485"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP9970911.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         8623 a         5539 c         5625 g         8510 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgcaacggt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acattgtcac
      121 agtcccttac tattatttaa ctatggataa acaaaatgag gttctttact acccctagat
      181 atctagcaaa atagctgatc tttttgctag tttatcttct tgggacggat aaacttccaa
      241 taatcccgta ttttttcttc aagtagtaac ggattgctaa agcagttgcc ccgataatga
      301 taactactac tggatctaat actggattga taactggtgg caatagtgtt gtcgcagaaa
      361 acagtacgac ccaaagaagt gtcgttccac ctaggataag caatgtctta cccattcctg
      421 gtttcttgct tcgatctgct ccgggatatt catattgata gatgtatttg tacatcatat
      481 aaaagaccca gccaccgatt gcagaaccta gaacgagtgt agaaaagcca taagctggcg
      541 ttgttccacg tgaaaacaat ccggtaatcg caatcatgat acttaataga ccaagcaaca
      601 gcaacgtatt atctaaccac atcaataaag gggtggatga attttttact tctgctggct
      661 tattgatgat tgcttccgtt ctttcagaga ctgtcccaaa caattgacgg gctgttcttc
      721 cgcctttttg ttctgcaact aaagtaggca gaatgtcatg aagggctttt actttttctt
      781 cttcagacaa attcgctgct tctaaagatt ttttcaaatc aaaaatgtac tgttgatttc
      841 ttttggttaa ttttgtttct aaactattgt tttcttttac gattgctcgt aattgttctg
      901 gttccattat ctaatagtcc tccttagccg ttccttttct caacggtcgt cacaaaagac
      961 attctaccat attctacagc tagtttgata gcccttttac acattaaagc ggaataacat
     1021 tacgtcgccg tcttgtacga cataatcttt tccttctaat cgaacacgtc cagcttcttt
     1081 tgctgcatgc atgtttccgt aatgatccaa gtcttcaaaa gagacagttt ctgcacggat
     1141 aaatccgcgt tcgaaatctg aatggatgat tcctgctgct tgtggtgctt tgattccttt
     1201 tttgaatgtc caagcacgta cttcttgttc cccagcagta aagtaagtag ctaagcctaa
     1261 caaatcataa gcagcacgga tcaactgatc caaacctgat tcttcgattc ctaaagcttc
     1321 taaaaattcc gctttgtctt cttcatctaa ttctgcgatt tcttcttctg cacgagcact
     1381 gaccacgatc acttctgcat tttcttctgc agcaaagttg cgcacttctt gtacataatg
     1441 attcgcgtct gcatctgcta cgtcatcttc tgagacattg gctacataaa gaattggttt
     1501 tgtcgtcaat aaaaatagtg atttaacgat tttttgttct tcttcagtaa attcgatcgt
     1561 tcgggcagat tttccttctt ccagtacagg tttgatttta tccagtacag ctagttctgc
     1621 cactgcatct ttatctttgg ttttagcaac ttttgctaca cgagtatagc gtttgttgat
     1681 agattctaag tctgctaaca ctagttccaa gttgatcgta tcaatatctt ctagtggatc
     1741 aacacgtcct tctacgtgag taatattatc atcatcaaaa cagcgaacaa cgtgacaaat
     1801 agcgtctact tggcggatat ggcttaagaa ttgatttccc aaaccttctc ctttacttgc
     1861 gccttttacg attccagcaa tatctgtaaa ttcaaaagtc gtcggaacag tttttttcgg
     1921 atgtacaagt tctgttaagc gttgcaaacg ccagtcaggt acttctacca tccctacgtt
     1981 ggggtcgatc gtcgcaaagg gatagtttgc tgcttctgct cctgcttttg tgatcgcatt
     2041 aaacaaagtt gattttccta cgttgggcaa cccaacaatt ccagcagtca atgccataaa
     2101 tttattcact ctttctctgc atttttttca attacttttt ttaatttctt ttcaaaatct
     2161 cgacgggtca tcatgacgat atgtccgcaa ttcatacatc tgattttgat atctgcgccc
     2221 attcgaatga cttcccagcg attcgtctgg caagcgtgag gttttttcat ttcaacgata
     2281 tcgtgcaagt cgtacataaa aatagcctcc tttttgttta ttcttcatca aattgaatat
     2341 tcaagatatc aagaattcga gtcaaatctt tttgagaaag atattcaatc tcgatcttac
     2401 ctttcccatt tttttcttgg atagctacac ttgtaccaaa tttatccatc aaacgatctt
     2461 cgctttctcg gatataatat ggtttttcct ttgctagcct tgggattttt ttcttttctt
     2521 ttccatcatt ttgatttaac tcagaaacaa gctgttccag ttggcggaca gttaaatttt
     2581 cagttactgc acgattggct aatttcaaga tcaatgtttt attttttaaa cctaacagcg
     2641 tacgggcttg tcccatagat aaacgctgat cttgtaccat ttccttgaca agatccggta
     2701 aagaaagcaa gcgcaaataa ttggcgatat aaggtctgct ctttcctaaa cgactagcta
     2761 cttctgcttg tgttagtttc aggtttttca tcagcatctc atatgcttct gcttcttcta
     2821 acggattcag atcttctcgc tgcaagtttt ccagcactgc tacttgcatc atcgcttctt
     2881 cgtcaaagtc tctaacgata gcagggatcg tttctttttc agctagtttg gacgctcgaa
     2941 aacgtcgctc ccctgcaata atctcatatc cttttactgc ggaacggcgt acgatgatgg
     3001 gttgaaagac acctgattgt tcaatagaat tagctagttc ctgcaaagat acttcttcaa
     3061 atgttttacg gggctgatac ggatttggtc gcagctcatt caatggtagc tggacaacgg
     3121 tatcatcttt tacatccacc gtctctaagt tttctaaatc ttgaaacagt gcatcgattc
     3181 ctcggcctaa tcctttactt ttcttcacga cttaccactt cctttgccaa tgcttggtag
     3241 acttcagctc ctcgagaacg tggatcgtag tcgatgatcg gttttccatg actaggtgct
     3301 tctgataaac gaatatttct tggaatgatc gtttcgtaaa ctttttcgcg gaagtattta
     3361 cgtacttctt ctacgacttc atttcccaga tttgttcgtg catcatacat tgtcagtaag
     3421 actccctcga tctctaattc tggattaaag tgtttttgga ctaaacgaac cgtattcagt
     3481 aattggctta atccttccaa tgcgtaatac tcacactgaa caggtatcaa aatcgaatca
     3541 cttgctgtaa atgaattgat tgtcagatgg ccaagagaag gcgggcaatc gatcaaaata
     3601 taatcatact gactgcttac ttctgctaaa gatcctttta atcttgattc tcttgccatc
     3661 attgacgtca attcgatctc tgctcctgct aattggagag tcgcaggcac aatactcaaa
     3721 ttttcgtgtt cagtgatcaa agttgcctca tcaattggca attcattgac tagaacatca
     3781 tagatgtctc ttgtcacatc aggttttctg atcccgacac cactggtagc atttccttgt
     3841 gcatccatat ccactagcaa tacttttttg ccaaggctag caagacaagc acctagattc
     3901 accgtcgtcg ttgttttgcc gacgccacct ttttggttcg caacagatat tatttgtgcc
     3961 atctcttctc ctcctatttg attggctgct tattaggcag tccgggcttt cttgggtatt
     4021 tctttggggt tggtttcttt ttttgaataa cgatgatatg ccgttgatca gagacattcg
     4081 gcaaggtaac cgcctcttct ttgaggaatt taccacctaa taaagcaatc gcttgttttg
     4141 cttctgtcaa ttcctcttca ctttttgctg ctttcaatgc ataaaaatag ccttcttgtt
     4201 tagccaaggg caaacacaat tccgctaaca cattcaaacg agcaaccgca cgagcagtga
     4261 caaggtcaaa tgatgcacga aagtgaggat tttgcccaaa tgtttctgcc cggtcatgat
     4321 ataaggaagc atcgattcct aataatcggg ttaactcaga taagaaggtt attcgtttat
     4381 tcagagaatc aacgatcgtt atctgcagtt ctggaaaggc aatttttaaa ggtatgctag
     4441 gaaaccctgc ccccgaacca acatcgcata aagataaagg ttgcgtaaag tctacttcaa
     4501 atgctaatgt aagtgaatcg taaaaatgct tcagatatac gtctttttca tccgtaatag
     4561 ctgtaaggtt catcttttcg ttccattcga ctaatgtccg ataataaata gcgaattgat
     4621 tcatttgatg atcggtaagc tctatccctt tttctgccaa ggattggcga aattcttctg
     4681 gtatcataaa tattcctttc attgtgaaac agcaattgtt tcacagattc tctccctact
     4741 ttaacgcaaa atctctagac atgcaatcct tccatatgga ttcttaaaaa tttgaaaagt
     4801 aagaaaagcc gatagacttt ttctttctag tatgactcat aaaatataca cagaagagag
     4861 aactagcttt gagcaatttc tgcaaaaact agttctctct tctatttatt attacgtatt
     4921 acgctggttc actgactttc gcaatcttcc cttgttcgat atagaccatt aggatactta
     4981 ggtctgcagg gtttacgcca ctgattcggc tagcttgggc aattgtttct ggttggattt
     5041 tctgcaattt ttgttttgct tctgttgcta atccgttgat tgctgcgtag tcgatgtttt
     5101 ctggaatctt tttcgcttcc atgcgtttta atttttctac tttttccatg gcttttttga
     5161 tgtagccttc atatttgatt tggacttcta cttgttcgat ttcttttcgt gtcaattctt
     5221 cattttctgg aataaaccgt agaagttctg cataagtaat ctcaggacgt cttaaaaagt
     5281 cgcttgctag tacaccatct tttaatggtg ccgcattaat cgttgctaaa aagtcttgga
     5341 cttcttctgt tggtttgatt cgtacattca tcaaacgttt gatttcttgt tcaactgctt
     5401 cttttttctt caaataattc ttgtattgat cttcttttac taagcctaca cggtgaccca
     5461 tttctgttaa tcgcaaatct gcattatcat gacgtaaaag cagacggtat tcagcacgtg
     5521 aagtcagcaa acgataaggt tcgttcgttc cttttgttac caaatcatcg atcatgacac
     5581 cgatgtaacc gtcacttcgt ttgatcacta aaggttcttt tccctgaact ttcaaagctg
     5641 cattgatacc ggcaatcagc ccttgtccag ccgcttcttc ataaccgctt gtaccatttg
     5701 tttgtccagc tgtataaaga ttttcaacga ctttagtttc taatgtcgga cggagctggt
     5761 gtggctctac tacgtcatat tcgatagcat atcctgtacg catcatttct actttttcta
     5821 atccagcaat agaatgaagc atgttgactt gaacatcctc tggtaaagaa gtagataaac
     5881 cttgaacata gacttcttcc gtatttaagc cttctggttc caaaaataat tgatgacgtg
     5941 gtttgtctgc aaaacgcacg atcttatctt cgatggatgg acaatagcga gcgcctaccc
     6001 cttcaacgat ccctgtaaac attggggcac gatgtaagtt gtctcgaata atttggtgag
     6061 tcgtctcacc tgtataggtc agccagcaag attcctgatc tagtttatag gcactatctg
     6121 gtgtgctgaa gctaaaatga ttcggctctt tgtcaccagg ctgctcttct gtaacagaat
     6181 agtcgatcgt acttgatttt actcgaggcg gtgttcctgt tttgaaccgt tcgatagcaa
     6241 agccaagttc ttttagatga ttcgctagtc caatagaagg ttgagagtta ttcggacctg
     6301 atgaatattt caattcaccg ataatgattt ctccgcgaag tgctgttccg gctgtaataa
     6361 caactgcttt ggcccggtag ctcgctcctg tggacgtcac tactccatga caaacaccat
     6421 cttcgacgat cagttcttcg acgattcctt gtcttagcgt caaatttggt tctttttcga
     6481 tcgtatgttt catttctgtt gaataagcat gtttgtctgc ttgtgctcgc aatgctcgga
     6541 cagctgggcc ttttcctgtg ttcaacatac gcatttggat ataggtttta tcgatattct
     6601 tacccatttc tccgcctaac gcatcgattt ctcgaacgac aacgccttta gctggtccgc
     6661 ctacagaagg attacatggc atgaatgcta ccatgtccaa atttattgtc agtaataatg
     6721 tttttgatcc catacgtgca gcagcaagtg cagcttctga accggcgtgt cctgcgccaa
     6781 caacgatcac gtcatacgca tctgcttcat aatgttccaa tcgtttgttc ctccttttat
     6841 tttcctaaac aaaattgact gaataattga gtgattagct catcttgtac actgtcgccg
     6901 acaacttctc ctagatagtc ccagcaacgc gtcatatcta tttgaacgag atccacaggc
     6961 attcctgaat caatacctgc aatgacttct tcaagtgaga gggaagcttt ttctaacaac
     7021 gcgatatggc gggtattaga aacataagta gcgtctcttt ctcctgtttc tccgctgaag
     7081 aataaatcag aaatagctga ttccaaacga tctagtccgt cgtttttagc tacggaaatt
     7141 gaaaaaactg gttcattttc gatcaatttc ttcaattttt cttgttccag ctgtgctggc
     7201 aaatctgttt tatttagaag aatgatccgt ttcaatccac ttgtcgcttc caataattgt
     7261 tcatcttctg ctgtcagtgg ttcgctttgg tttagaacaa gtaagatcaa gtctgcttct
     7321 gctaatgctt ttcggcttct ttcgaccccg atccgttcca ccacatcttc tgtttcccga
     7381 attccagctg tatcgattag cttgagcggt accccccgaa cattgacata ctcttcgatt
     7441 acatctcgag tcgttcctgc aatatccgta acgattgctt tttcttctct taacaaatgg
     7501 tttaacaaac ttgatttgcc gacatttggc cggccgatga ttgccgtact taacccttcc
     7561 cgcagaacct tcccttgctt ggatgtagca agtagcgctt ggattctttg ttggatcatc
     7621 tgcgcttttt caagcaataa cttcgtagtc aattcttcga catcatcata ttctggataa
     7681 tcaatattta cttctacttg tgctaatgtc tctaaaatct cttgtctcaa tgagcggatc
     7741 aaagcagaca gattgccgtc taactgatta agagctagtc ccattgcttt gtctgttttt
     7801 gcccgaatca aatccatcac agcttctgcc tgtgaaaggt caacacgacc attcaagaat
     7861 gctcgttttg tgaattcgcc tggctcagct aatcttgcac cttctcttaa caatagctgc
     7921 aaaatttgat tgactacaac gatcccaccg tgacaattga tttctactac atcttcccgc
     7981 gtaaatgtct ttggtgctct catcacggag accatgactt catcgacaag ctgattgctc
     8041 ttgggatcaa cgatatgtcc ataatggatc gtatgacttg gtacttctga taatcgtttg
     8101 tttccagatt gataaacttt atctgctagt tgcacagctt gatcgccact taaacgaacg
     8161 atactgattg caccttctcc aggtggggta gaaattgccg caatcgtatc aaattctaaa
     8221 gtaatttcag ccattttttc acttcctttt tcattttgcg cacaaaaaaa gtgcccactc
     8281 caccctttat gcacaataaa cggtgaactt cgcactttga ctgcttgttc ttgattaaat
     8341 tctggtcata gattatcaca cttgctataa ggaatcaagt gtttaaaaca attagatgaa
     8401 gttgagcctg ttctgcttcc attaccttat atttaattgt ccttggatgt ctctttgcgt
     8461 tctttggttt attcgaagag aagatactgt ataaacaagg ctttatcttt cttaaaaaaa
     8521 acggtacact aaatggtagc gctatctaaa ctatacactt ttgaaaggag aaaatatgtg
     8581 agcccgttca gatctgctgc attggaagaa gaaagtgacc ctcggatcaa cgatgtcgtt
     8641 ttatctacag ggaatatcaa ccagaaatat atcgtccgcg atatcgtttt tgttgtggaa
     8701 tgcttagaag ctgatttgtt tgacccatct tttcccaaag atgacctttt cttaacaacg
     8761 aagaggaaat taaagacgaa ggtacttgaa tatggtgcga atgcagtaat caactgtcat
     8821 tttgagtatc agcaaaaaaa acgcgacgga aagtattttc tggaactttt tgcttatgga
     8881 acagtgatcc aatttatcca aacaacgatt ggttaaatga aacagacaaa aaaagcggat
     8941 caaaactgaa gacagtttcg atccgctttt ttctatcaaa gttagtgact acttcatcgt
     9001 atactctgat aagaatgatt tctagtaaaa tatacttggt ttgaaaaact ttgtcaacgt
     9061 gatttattca aaaagtaatg gaatcaataa tattttttag caggttctac tactaaataa
     9121 cgataaggtt cctcaccttc cgaatgtgtt tttacatgtt catctttgct taatgcagca
     9181 tggatttgtt tacgttcaaa tgcaggcatt ggttctaaga aaacaggctg ccctgtccgt
     9241 tttacttttt cagccgtgcg atcagctaaa cgctgaatga tctcttggcg cttttcacgg
     9301 taattgccta cattgactac tacagacagc ttatttcttg ctaaacgatg gatgaagact
     9361 tgtgccagat attgcaatgc attcagtgtt tttccatgtt tcccaatcag tatgccttgc
     9421 ttttgtgttt ctaaatggaa aacaaccata ttatttgatt ctctttccat tttgacaaga
     9481 gctggagcat caagctgttt tgaaatttca gtaagataaa ctgccaattc cgttaaagct
     9541 gcttcatcac ctagctcact tacttcttct gttttttctt caagaatcgt ttcgttgact
     9601 acctcgatct cagctgttgg ttcttcttcc aaaatttctt ctacagtctg ttctactgtt
     9661 tcagaaactt tttcgctgat acttggctgg atcgaaagcc gtgcattctt tcgacccatt
     9721 cctaggaaac ctttttttcc ttcttccagt atttcgattt cgacttggtc tttggccaaa
     9781 cccaaatcag ccagacctag ttggacagct tcgtctattg ttgcagcttc ataaatcggc
     9841 attagcatta cctcccctat tatttccgtt ttttcttagg attcattgct ttcttaagag
     9901 cacgttctct ttcgcggatt ctccgtgctt cttcttcacg ctcttttcga attttgaaag
     9961 ggttgttgat cagcagcgtt tgtacaactt ggaaggcatt ggaaactacc cagtataatg
    10021 aaagaccgct ggctaaattg acacccatca ctaaaatcat tacaggcatc acgaaattca
    10081 tgatcttcat tgatgcattc gattcaattt gactcatgct agataaatac gtacttgcaa
    10141 aagtaaagat tgctgctaaa atcggcaaaa taaatgttgg atccgcgttc cctaagttca
    10201 accataagaa atgcccttgt ttcaactctg gtacacgtga aatagattgc cataacgcca
    10261 tcaagattgg catttgtact aataaaggaa gacaaccagc atatggattg acgccatttt
    10321 ctgcatataa acgctgctgc tcttctctta acttactttg tgtttccgga tctcttgaac
    10381 tatattgttg ctgcagtgct tttaatttcg gttgtaattc ttgcgtttta cgcatgctct
    10441 ttgtttggaa atgcatcaac ggcaatagga tcacgcggat gatcagtgta aataaaatga
    10501 tccctatccc tgcattacca aatgacaacg ccttgatcgc ctcagcaaag taataaacaa
    10561 tataacgatc ccaaattcct gtactctgtg cgctgacttc cccagttccg caaccggata
    10621 agacgatgac caaactaacg ataccggcca tcaaaagtaa acgtttgtat ttcttcactt
    10681 actcttttcc tctctgtcga ttatttttgc aagttttaag acgtggacta aattggagcg
    10741 aacctcatca taagtcaacc ccttcacact tggtctagca atcacaataa aatccaactc
    10801 cggatcaatt tcttctttta tagaataaac tgctgctcgg atttttcgtt tgacttcatt
    10861 gcggtttacc gcgttgccga ccttttttcc tacagataaa ccaactcgaa aatgaggctg
    10921 tcccggtttt tccaaacggt aaacgacgaa tttgcggtta gcaaaagaat ctcccgtatg
    10981 gaacacggct tgaaactctc gttctttttt tactcgatac gcttttctca ttagttttct
    11041 ttccttttct atctctcact tacgctcata atcataccat aattacgatt gtcctttcat
    11101 catttcccta aaaaagagta taaaaaaaac cactgaaacc ccagtggcct aagctgaaag
    11161 aacttttctt cctttgcgac gacggctagc taacacacga cgtccatttt tagtactcat
    11221 acgtttacgg aatccgtgaa ctttttgacg tttacgttta tttggttggt atgttctttt
    11281 catatattcc acctccaaaa tgttcttatc tattttataa acacagacat actttgatag
    11341 tataacgaca aagtagtgat tttgtcaata taaaatagaa gatcgattgt taaaacttat
    11401 ccacaagatt atttttttct atgggagtcc tatgtggaaa aagttatgca cagggattat
    11461 ccacagataa taaatggctg gttttacaca gaaaacacac gtgtaaaaaa agtaatccac
    11521 aatcaggtga tttcctgtgt attccgtctc ctaactattg gtatatctat attttttatc
    11581 cacagacgca agaggaaatc tattgatttt tacgagtttt ccacctgtgc attagtgttg
    11641 tggaaaactt ttttttgaca actgtgtttt tctctttttt ctttttgtcg attgtgtgga
    11701 taattgtaat taaaaaattt ttttcttcgg tttctgtgga aaactattga aaaaaatgct
    11761 aaattagtac tagttattca ctttttaatg aaggaggagg ccttatatgg tatccctcga
    11821 tgctttatgg aatgaattaa aagcaacata ccaaaaggat ttatcgcctg ctagctataa
    11881 tacatggatc gaaacagcga accctcgaac ccttgatcaa aaccagctcg ttgttgaggt
    11941 tcctagcaag attcataaag aatattggga aaaaaacctg gcgacaaaaa tcgttgaagt
    12001 cggttatatg ttatcaggaa atgagattat ccctcgcttt attactggcg aagaagcaga
    12061 gcaagaagaa gttatagaag aaaaaaatcc aaaagttgtg gcacctagtc cgctaaaaaa
    12121 agccatgctg aaccctaaat atacctttga tacgtttgtc attggtaaag gaaaccagat
    12181 ggctcatgca gcagcgctcg ttgtagcaga agatccaggt tctatttata acccgctctt
    12241 cttttatgga ggcgtgggac ttggtaagac tcacttgatg catgcaattg gtcatcaaat
    12301 gctgcaaagt cagcctaatg ccaaagtaaa atatgtaagc agtgaaactt ttgctaatga
    12361 tttcatcaat tcgatccaaa acaaaacagc agaagagttc cgtcaagaat atcgaaatgt
    12421 tgatttactg ctagtggatg acattcaatt ttttgcagaa aaagaagcta cgcaagaaga
    12481 gtttttccat actttcaatg ccttgtacaa tgaagggaaa caaatcgtac taaccagtga
    12541 tcggttgcca aacgagattc cgaaactgca agaacgcctc gtttctcgct ttgcatgggg
    12601 gctgtctgtt gatattacac cgccggatct tgaaacaaga acagcgattt tacgtaaaaa
    12661 agcggcggct gaacgtctag agatcccaga tgacacacta agctacatcg caggacaaat
    12721 tgattctaac attcgtgaat tagaaggtgc gcttgttcga gtgcaagctt ttgctgcgat
    12781 gaatagtgaa gatatttcga ctagtctagc tgcagacgca ctgaaaacgt taaaatcagg
    12841 aaaaggacat cctcaattgt caatcctgca gatccaagaa gaagtggcaa agtattatca
    12901 catccaattg aaagatatca aaggaaagaa acgcgtcaaa tctattgttg ttcctagaca
    12961 aatcgctatg tatttggcac gtgagctcac agataattct ttaccgaaaa tcggagctga
    13021 attcggtggc aaggatcata caacggttat ccatgctcat gaaaaaatcc agcagctgat
    13081 ggattctagt gtcagtatgc aaaatgaagt atcagaaatc aaaaactttt tactaaatta
    13141 atttgtcgat tctatcaact gaaaatagca tgtggataaa agaaaaaaac gttcaaaagt
    13201 tatgcacact ttttccacag ctgttttttc gtttctctct tacgtttttt gagttttcca
    13261 cagaatcaac atgccctatt acttttatta ttctttttaa ctaataataa ataaacaaac
    13321 attcaaagga gatacatcat gaaagttact ttaaaccgag ctagctttat gcaggaattg
    13381 caaactgttc aacgagctat ttcaagcaaa accacgatcc ctattttgac aggtgtaaaa
    13441 atcacactga cacaagaagg tttgactttg acggggagca acgctgatat atcaattgaa
    13501 acttttttgt ctgttgaaaa cgaaaaagca aatatgcaaa tcgaatctac tggttccatt
    13561 gttttacaag cacgtttctt tagcgaaatc attcggagac ttcctgaaga aacatttact
    13621 ttagaagttt tagaaaataa acaagtagcg attacttctg gaaaagcgaa ttttatcgta
    13681 aatggattag atgcagataa ctatcctcat cttcctgttg tcgaaagcca taaccagatg
    13741 aaattacctg tacacgtatt gactaaacta atcaacgaaa cagtttttgc tgtctcacaa
    13801 catgagagtc gtccaatctt gacaggtgtg catttcattt tatctgataa ttctttattg
    13861 gctgtagcta ctgattctca ccgtctaagt caacgcgtga ttccagtaga acaagcggct
    13921 gatcattttg atattgttat tcctggaaaa agtttgatcg aattatctcg ctcattaaca
    13981 aatgaagaag aaatcgtcga aatcagcatt atggaaaacc aagtgttgtt caaaacagaa
    14041 acgatgtatt tctattcccg tttgctagaa ggaaactatc ctgataccaa tcgtttgatt
    14101 ccttcaagtt ttaatacaga ggttgaattt tctgttccta gctttttagc ggcgatcgaa
    14161 cgggcttctt tactttctca tgaaggccgt aacaacatcg ttcgtttatc tattcgtcca
    14221 gatgcggttg ttttatacgg aaattcgcca gaaatcggga aagtagaaga aagcttgagt
    14281 tatacagcaa gcagtggcga tccattagat atttctttca atccggatta tatgaaagca
    14341 gcgttgcgtg catttggaga tatgagcatc aaagtgaaat ttatctctgc tattcgtcca
    14401 tttacattgg aaccaacgga agatggcgtt caatttattc agctgattac tcctgtacgt
    14461 acaaattaac aatgattttc attgttcttg aaaatttaat tgaaaagaaa tagaaaaaat
    14521 aaaattttaa acagaaggcc ctaaaatctc ttttaaggct ttctgtttat ttttgtttaa
    14581 atcacctttt ttagattaaa tcgtcttata tggaaaaata tatggataaa tttgttttat
    14641 tgcgaaaaaa aaggtataat agagtatact tttaataatg aaaatgaggg atgggcattg
    14701 aaacaacaag tcattctaaa tacggaattc atgacgttgg gccaaatgtt gaaagaagtc
    14761 acagttatcg gtagtggcgg acaagcaaaa tggtatttgg cagaaaacac tgtgttagtc
    14821 gatggcgaac cagaaaatcg tcgtggacgc aaactttatc ccggcatgat ggttgaagtt
    14881 ccagaagtag gaactttttt tatggtaaaa aaaggaaacg acgataatga ggctgaataa
    14941 gctgtattta aaaaattatc gaaattacga agagctgaac atcgaatttt ccaagaacct
    15001 cgtcatcttt ttaggagaaa atgcgcaagg aaaaacgaat cttttggaaa gtatctacgt
    15061 attagctatg acgcgaagtc atcggacaag tagtgagcag gaattgattg gctggactga
    15121 cgatcaggcg atgattcaag gagagatcac aaaaggttca tccactcttc cattagaaat
    15181 cttattatct aaaaaaggcc gaaaaaccaa gatcaaccat atcgaacaaa aaaagttaag
    15241 tacttacgtg ggacaaatga atgtcatttt atttgcacca gaagatcttt ctttagtaaa
    15301 aggcagcccg cagatcaggc ggaagttttt agatatggaa ttaggccaga tcaatccaat
    15361 ctatctttat gatcttgtcc aataccaagc aattttgaaa caaagaaacc agtatttaaa
    15421 gcaattgaat gaaaaaaaac aaactgattt gctttatttg gatattttgt ccgaacaact
    15481 agcagcgttt ggcagcaaag tattgaaagc aagagcacag ttcgtcaagc gattggagtt
    15541 ttgggctaat tctttgcatc agcaaatcac ccatcaaaaa gagcagctgg aaatcgagta
    15601 tctcaccgca gtcgattctt tagaaacaca tacgcaagag caaatccaag aacaatttct
    15661 cgcacttttg aaccaaaata aaaagaaaga cctttttcgg ggaacaacga cagttggtcc
    15721 tcatcgtgac gatctttcct tttttattaa tcagaaaaat gtgcagactt atggatcgca
    15781 aggacagcaa agaacaactg cactaagtgt caaattagca gaaatcgatt tgattaaaga
    15841 agagacgggg gaatatccaa tccttttgtt agatgatgtg atgagcgagc tggatgatag
    15901 tcgtcaactc catctgctag aaacaatcga aggaaaagtt caaacgttct taacaacaac
    15961 gacacttgac catgtaaaaa ataaaatgac tgttgaacct gagatttttt atgttcaaca
    16021 agggaaacta aaaggagaag aaactgaatg acagaagaaa gaagtttagt agaacgcgct
    16081 aaagagtatg atgccagtca gattcaagta cttgaaggac ttgaagccgt tcgtaaacga
    16141 cctggcatgt atatcggttc gactagttct gagggtctgc atcaccttgt ttgggaaatt
    16201 gtcgataatt cgatcgatga agtattagca ggttttgcaa cgaaaatcca tgtgattatt
    16261 gaaaaagaca acagtatcac cgttattgac gacggtcggg ggatacctgt agatatccag
    16321 gcaaaaactg gacgtcctgc agttgaaacg gtctttactg ttttacatgc tggaggaaaa
    16381 ttcggcggtg gtgggtacaa agtttctggt ggacttcacg gagtaggatc ttctgttgtt
    16441 aacgcgcttt ctacacagct tgatgtcaaa gtatacaaag aaggaaatgt gtactatcaa
    16501 gaatatcgtc gtggagctgt agtggacgac ttaaagatta tcgaacaaac ggatcgccat
    16561 ggaacaacgg ttcactttac gccagaccct gaaattttta cagagacaac tgagtttgat
    16621 ttttcaaaat tggctacccg gatccgtgaa cttgctttct tgaaccgcgg gatgcgtata
    16681 tcgattgaag acaaacgaga agaagaacca gtcatcaatg aataccatta tgacggcggg
    16741 attaaaagtt atgtcgaata cttgaatgca aacaaaaccg tgatttttcc agaacctgta
    16801 tatttggaag gtgaacagca agatatcgca gttgaagtct ctatgcaata tacagacggc
    16861 taccattcca atatcatgag ttttgccaac aatatccata cgtatgaagg cggaacacat
    16921 gaatcaggat tcaagactgc tttaactcgt gtgataaatg attatgcccg aaaacaaaag
    16981 ctgatgaaag aaaatgacga caacttgacc ggtgaagacg tgcgagaagg attgacagct
    17041 gttatttcta tcaagcatcc tgaccctcaa ttcgaaggac aaacaaaaac aaaattgggc
    17101 aattccgaag tacggacagt cactgatcgg ttatttagcg aacatttcat gaaattcttg
    17161 ctggaaaacc caagtgtggg ccgtcaaatt gttgaaaaag gattactagc ttccagagct
    17221 cgtctagcag cgaaacgtgc gcgtgaagta acgagacgta aaggtgcttt agagattagt
    17281 aatctgccag gaaaattagc ggactgctct agcaacgatc ctgaaaaatg cgaattgttc
    17341 atcgtcgaag gagattcggc cggcggatca gctaagcaag gtcgtaatcg agagtttcaa
    17401 gccatcttgc ctattcgtgg gaaaatcttg aatgttgaaa aagcaagcat ggacaagatc
    17461 ttggcaaatg aagagatccg gtctttattt actgctatgg gtacagggtt tggagaagat
    17521 tttgatgtat cgaaagctcg ctaccacaaa ttagttatca tgactgatgc cgatgtcgac
    17581 ggcgcgcata tccgtacatt gctgctgact cttttttatc gttacatgcg tcctattgtt
    17641 gaagctggat acgtctatat tgctcagcca cctttatacg gggtaaaaca agggaagaat
    17701 attacgtatg tacaaccagg aaaaaatgca gaagaagagt tgaaacaagt cgttgcttca
    17761 cttcctgcct caccaaaacc aagcgttcag cgttataaag gacttggaga aatggatgat
    17821 catcaactat gggaaacaac tatggaccca agcaatcgga tgatggcccg cgtcagtgtg
    17881 gacgatgcta ttgcagcaga tcagatattt gaaatgttga tgggggatcg agtagaaccc
    17941 cgtcgtgcat tcatcgagga aaatgcacac tatgtgaaga acttagatat ttaatcatga
    18001 aggaggaaaa agaatgagtg aagaaatcag agaaaacatc catgatgtca atctaaccag
    18061 cgaaatgaaa gactccttca tcgattatgc gatgagtgtt attgtcgcac gtgcacttcc
    18121 tgatgtcaga gatggtctga aacccgttca tcgccgtatt ttatacggca tgaacgaatt
    18181 aggtgtaaca ccggataaag cccataaaaa atctgccaga atcgtcggag atgtcatggg
    18241 taaataccat cctcatggag actatgcgat ctatgagtca atggtacgga tggcacagcc
    18301 attcagctac cgttatatgc tagtggatgg tcatggaaac tttggttctg ttgacggtga
    18361 cggtgccgcc gctatgcgtt ataccgaagc acggatgagc aaaatcgcta cagaaatgct
    18421 gcgtgatatc aataagaaca ctgttgattt ccaaagcaac tatgatgata cagaaaaaga
    18481 accagttgta ctacctgctc gcttcccgaa tcttttagtg aacggaacaa cggggatcgc
    18541 cgtaggtatg gccacgaata tcccacctca taacttgtct gaggtagtag cggctatcga
    18601 tttgctgatg gataatcctg atgtgacgac caatgaattg atggaagtat tacctggacc
    18661 agattttcca acaggtggtt tggttatggg aaaatcagga atccgaagag cgtatgaaac
    18721 aggtaagggt tctattaccg tacgtgcaaa agttgagatt actgagatgc caaatggtaa
    18781 agaacggatc atcgtgacag aattgcctta tatggtcaat aaagcaaaat tgatcgaacg
    18841 gatttctgaa ctgcacagag acaagcgtat cgaagggatc acagatttgc gggacgaatc
    18901 ttcccgtgaa ggtatgcgga tcgtcattga catccgtcgt gacgccagcg catctgttat
    18961 cttgaataac ttatataaat tgacatcctt gcaaaattca tttggattca atatgttggc
    19021 gatcgaaaaa ggtgtgccga aagttttgag cttgaaacaa attcttgaaa actatatcga
    19081 acatcagcgc gaagtcatca caagacggac agtctttgaa aaagaaaaag cagaagcaag
    19141 ggcacacatt ttagaaggtt tgcgtattgc tttggatcat atcgatgaaa tcattgcgat
    19201 tatccgcggg tctaaatcag atgacgaagc aaaagcgacg atgatcgaac ggttcgattt
    19261 atccgatcgc cagtctcaag ctattttaga tatgcgttta cgccgattga ccggtttgga
    19321 acgggaaaaa atcgaaaatg aataccaaga actcttaaaa ctgattgaag atttaactga
    19381 cattctggca agaccagaac gtgttacaga aatcatcaag aatgaattag cagacttgaa
    19441 tcaacgattt ggtgataaac gacgcacaga attactagtc ggtgaggtct taagtctgga
    19501 agatgaagat ttaatcgaag aagaagaaat cgttattaca ttgacaaaca atggttacat
    19561 caaacgtttg gcaaataatg aattcagagc tcaacgaagg ggtggacgtg gtgtccaagg
    19621 aatgggtatc cacaatgacg acttcgtcaa aaatctggtt tcttgttcta cccatgatac
    19681 attgttattc tttacgaata acggaaaagt gtatcgggca aaaggatacg aaattccaga
    19741 atacgggcgc acagccaaag gaataccaat tatcaattta ctaggtatcg attcgagtga
    19801 aagcatccag gcaatcattg cggtgacggg agaagcagaa gaaggccact atctcttctt
    19861 tacgacgcgt caaggtacag taaaacgtac ttctgtcact gcttttgcta acatccgaag
    19921 taatggtctg atcgcaattg gtttgaaaga agacgatgag ctcgtcaatg tgctactgac
    19981 agatggtcaa tctaatatca ttattggaac acataacggc tactctgtca ctttcaaaga
    20041 agaagcagtc cgcgatatgg gacgtacagc ttccggggtt agaggaatac gcctgcgtga
    20101 ggaagattat gtcgttgggg cagccttaat gaaggaagat caagaagtat tagtgatcac
    20161 agaaaaaggt tacggaaaac ggacaaaagt atcggaatat ccagtgaaag gccgcggagg
    20221 taaagggatc aaaacggcga acatcactga aaaaaatggt ccactagctg gattgaccac
    20281 tgtttctgga gatgaagaca tcatgctgat tacagataaa ggtgtcatta tccgtttcaa
    20341 cgtatcgact gtttctcaaa ctggacgttc aacattaggc gtacgtttga tgaagatgga
    20401 agctgatacg aaagttgtca cgatggcagc agtcgaacca gaagctgcag aacaaacagt
    20461 tgaagaacaa ggatcagaag aatagccatc aagactaatt gtgtaagcta gcaagaatga
    20521 ccgattaaaa aatctggtta ttcctgctgg cttttttgat tagttgaatt tctcttaaaa
    20581 tattcgaaac tgttcattct gtattgcttt tgaccaaaat agagtgtata atcagagctt
    20641 gtgagtagtc taattaatta gataactctc cttgctctta ggaacctaag agccgaaagt
    20701 ccacagggag gtgaatatca atggaaaaca cgaaatatga aatcatgtac attattcgtc
    20761 caaacattga tgaagaagca aaaactgctt taatcgaacg tttcgatgca atcttgaaag
    20821 ataacggagc agaagttatc gaatcaaaag attgggaaaa acgccgtttc gcttatgaaa
    20881 tgaacggtta ccgtgaaggt atctatcaca tcgttaaagt atcttctcca tcatcagcaa
    20941 atgctgtcaa tgaatttgac cgtcttgcta aaatcaacga cgatatcatt cgtcacatga
    21001 ttgttaaaga agaagcttaa ttgttgtttc atgtgaaaca atagaaaacg atgagaggag
    21061 atgactattt tgattaataa tgttgtatta gttggacgtc tgacgaaaga tccagatttg
    21121 cgttacacag caagtggtac ggcagtcgca acatttactt tggctgtgaa tcgtaatttt
    21181 acaaaccaaa gcggcaatcg cgaggctgat tttatcaact gtgtgatttg gcgcaaatca
    21241 gcagaaactt tagcaaacta tgctcgtaaa ggaacacttt taggtgttac tggacgtatc
    21301 caaactcgtt cttatgataa tcagcaagga caacgtgtat acgtgacaga agtagtagct
    21361 gacaacttcc aattgctgga aagtcgttct gcatctgaaa atcgccaaca aagcggcgga
    21421 ttccaaagtt ctggtcaatc agctggcgga tttggcggaa acaataactc taatcaaaca
    21481 tctcaatcat ccaacggtat gcctgatttc gaccgtgata cttctgaccc attcggctca
    21541 agttcaacga tcgacatttc agacgatgat ttaccattct aaacgaattt gataggaggg
    21601 aaatagaatg gcacaacaaa gaagaggcgg acgtaaacgt cgtaaagtag actacattgc
    21661 tgctaaccat attgaatata tcgattataa agatatcgaa ttactaaaaa gatttatttc
    21721 agaacgcggt aaaatcttgc cacgtcgtgt aactggtaca ggcgctaaaa accaacgtaa
    21781 aattactgtt gcaatcaaac gcgctcgtat catgggcttg ctacctttcg ttagcgaaga
    21841 ctaataaatc ctaggaattg aatgactgat ccttttgatc agctattcaa ttcctttttt
    21901 tctgtaaaaa taacctctat tcgtttagtc gctcgaatga cgaaagttat ggtaaaatca
    21961 aattagtcat atatgggagg aaaattatgg aaaataaaaa caatcattta tccccgaaac
    22021 ctctcttcct tatcctgttt atattaatcg aagtattggt agtcttaatt ttaccatttc
    22081 gttttgttag cgcttcggtc attctattga tgaatttatg gggtgttcgc tattggttga
    22141 aaatgaaacg ttggcttatt ttaaataaac gtgaaaaaat ccagcaggct tcgactgtag
    22201 ctgaaaccaa tctagcttat gtctcagaag tcatgcctgt aggagtgatt gcttatcagc
    22261 catcaagcca aaaaatcgaa tggctcaatc cattagcatt gaaaatcaaa gatcaaagtg
    22321 gattaacaga ggaagaattg ctagatactt tttttactgc aaaagaaaaa aagaaaaacc
    22381 aagtctatct agcagataaa acatttcagt ttgatatgga tacagtaaag ggtgttgttt
    22441 attttaatga tattacccaa aaccagttgt taaaactcca tcagacgaac agtcagccgg
    22501 tgattggtat tttatctatt gataactatg cagatgccat tgataaattg gatgacaaag
    22561 agatttctta tttgaactct ttcgtcacta cactagtttc tgactggatg aatgaatacc
    22621 atgtcttttt caaaagaatc aacgcagaac ggtttttctt cgtagcacag atgatcgatc
    22681 tgaaaaagat gatggataaa aaatttgatt tgctggatcg ctttcgagca gaagctgaaa
    22741 aacagagctc gcctatcacc cttagtatgg ggattgctta tggctcaaga accctcgatg
    22801 aaatcggtgg agaagctcag aataatttag atgttgcttt agtccgtgga ggcgaccaag
    22861 ttgtcgtgaa agaggtaaaa gaaggagcaa aacctgttta ttatgggggg aaatctgcta
    22921 agaatgtcaa acgcacgcgt gttcgttctc gagcgatgag taccgctttg cgtcggattt
    22981 ttgatgaaac aggcgatgtg tttatcatgg ggcacaagtt tccggatatg gatgctttag
    23041 gctctgcttt tggtgtcagt tgtctggcac gcttcaacca gaaaaaagct tacatcgtta
    23101 tcaacgagaa cgagatcatt ccagatgtag aacggtgtct tgaagaaatc cataagcatc
    23161 ctgaattaga aatgcaacta atctctccag aacgtgccat agagctgaaa aaagaagagg
    23221 acctcctcgt gatggtggat taccataaac cttctctatc gatctctcag ggcctatatg
    23281 agcaatttga taagattgtg atcattgatc accatcgtcg tggggaagag tttccaagta
    23341 aacctctgct gacttatatc gaatcttcag cttcttcagc ttcagagcta gtttctgagc
    23401 tgatccaata ccaaagcagc aaaacaaatc gcttagatcg ttttgaggcc acattattgc
    23461 ttgcagggat ggttgtagat acaaatagtt tcaatgtccg tacaactgca cgaacttttg
    23521 acgttgccag ctatttaagg tcttgtggag cagacgcatc cttagtacaa tatttattga
    23581 gctcggattt agcgacatac ttggagatca gcaatttgat tgctggaagc gactatgtga
    23641 cttctgatat tgttgtggcg gcagggacag acgaaaaaga gtataatagt gttatagcag
    23701 caaaaacagc agatacattg ctttcgatga tcaatatcaa tgcagctttc gtcattacaa
    23761 agcggacaga cggcttgatt gggatcagtg ccagaagtac cggcacgatc aacgtccaaa
    23821 tcatcatgga atccttaggt ggcggaggac acttcacaaa cgcagctact caactggaaa
    23881 atacgacagt tgaggaaaca aaacagaagc tatttgctgc aatcaaagaa aatatgaatc
    23941 aaatatatga taaggagtaa tccaaatgaa agtaattttt ttacaagatg taaaaggaaa
    24001 aggtaaaaaa ggggacatca aagaagttcc agcaggttat gcacaaaatt atctaatcaa
    24061 aaataacttg gcaaaagaag caacgaaaag cagtatcagt cagttagcag gacaaaagaa
    24121 agcacaagaa aaacacgaag ctgaaatctt ggaagaagct aaaaagttaa aagaattctt
    24181 ggaaaaagaa gatactgtag tcgaattgaa ggcgaaagca ggggaagaca gccgtttgtt
    24241 tggttcgatc ccttcaaaac aaattgctga agggttaaac aaacaataca aaatcaaatt
    24301 agataaacga aaaatcgaat tagaaaatcc gatccgttca ctaggctaca caaacgtacc
    24361 tgttaagctt catcatgagg tgacggctac gatcaaagtt catgtcgtac aagaataaca
    24421 aatcttgata gcgatgttca gctggtaagt ataaccagaa ggaaaaaaga caaaccgaaa
    24481 tttatctgag aaaacagctg cagcaatgaa agcaaaagct gcaaatcgat ttcagattga
    24541 tttcggtttc ttttttggga acagcaaaat agcataaaga aaacgagtat aataaaagag
    24601 aactattttt ttattacact ttcggtacga atgatgactt ttctctcatt ttcttgtaaa
    24661 atgaagcaat tgaagacaag gagcgatacg tatgaatgaa gtgtggcagg atcgaatccc
    24721 accgcaaaat attgaagctg aacaagcggt tttaggctct gtttttcttg acgcagaaac
    24781 gatcattgat gccatggagt acatagagcc aaaagacttt tatcgtcgtg gccatcaaat
    24841 tatttttgag acaatgatcg agttaaatga tcgtaatgaa gcgattgacg tggttactgt
    24901 caaagaccgg ctggaacaag ccaatttgtt agaagatgcc ggtggtttaa gttatctctc
    24961 agacttggca ttagcagttc cgacagcagc aaatatcgtc tactatgcga aaatcgttga
    25021 agaaaaatca ttgctgcgta cattgatcca gacagcaaca ggaatcgtta cgaaaggatt
    25081 cgagcaagga gaagaagttc aaacaatttt agatgatgcc gaacgcagta tcttggaggt
    25141 ttcggaaaaa agaaatcgaa gcggcttttt atctattgct gaagtactga actccacgat
    25201 tgaaaatatc gatcgactag ctcaaaacaa tgaagaaatc acaggtctac caactggcta
    25261 tcaaggtctg gataaaatga cagcaggttt ccagcccgaa gaactaatca ttcttgctgc
    25321 acgtccagcg gtaggtaaaa cagcgtttgc attgaatatt gctcaaaatg taggaacaaa
    25381 aacagatcgt tctgttgcga tatttagttt ggaaatgggg gcagaatctc ttgtaaatcg
    25441 gatgctttgt gcagaaggtt cgattgaggc cagccattta cgtacaggtc agctttcgga
    25501 agaagaatgg tcgaatctga tcgttgcgat ggggagtctt tctcgagcaa atatttttat
    25561 cgatgataca ccagggatca aaatctctga aatccgtgcg aaatgtcgga aactagcgca
    25621 agaaaaaggc aatctaggct tgatattgat cgattatttg cagttgattg aaggtaacgg
    25681 taaagagaac cgtcaacaag aagtatcaga tatctctcgc caactgaaaa aactggcgaa
    25741 agagctgaaa gtacctgtca ttgctctttc acagttatct cgtggagtag aacagcgaca
    25801 agacaaacga ccagtgctaa gtgatattcg tgaatccgga tcaattgaac aagatgcgga
    25861 catcgtagcc ttcttgtatc gagatgatta ttatgaaaga gaaggcggag atgacgatga
    25921 tggaaacgag ccaaaaaatg acaacgtcat cgaagtcatc atcgagaaga accgaagcgg
    25981 tgcacgagga acagttgaac tactgtttat caaagaatac aataagtttt cgtcgctttc
    26041 gccaagagaa gaattttaaa gaagaggttg tgagccttga gcaatcccga gagctctcgt
    26101 aagaagatgc aaggagtaac cgcaggaacg actagagtac ttaatttaaa cagatctcca
    26161 aagggattgt gacaaaagtc ttgtcacagt ccctttttcc gaataaacgg tgttcaatca
    26221 gctgaccctt ggtattagct cagcaaacgt caaaatatga ggaactattt tgacgtttgt
    26281 ttctctaata ctcaggtcaa aacgctgatc ttacgacctc ttttccttaa acggtgttct
    26341 cctgccaact cctcggtcac aagtcacaat atcatcaaac atgagaagca gttttttgaa
    26401 attgctcctt gtgactcaga gttaaacggc aggctcacaa cctctttctt aaacggtgtt
    26461 caaaagcttg catctgcggt aatacgccca atcaaacgaa aaatatgaga agctattttt
    26521 gtttggctgt tcttattact tgatgcagga cagctttttc acaacctctg attcacggtg
    26581 ttccaaaagt agcttcttcg aaaaataagt cgaaatttca caaaaatttg agaagcaatt
    26641 ttcgtacatt ccttcttatt tcttgaagct gaccacttct gttacaacct cttttttaat
    26701 aaccattcta aaaagataaa gttcgttttt taagcgtttt tataaaataa aaataaaaat
    26761 cattcgattt tatttgaaat atgtatctat tattgataca atggttcagg attaaataat
    26821 atagaacgag gtgttcacat gtcatctgta gtagtagtag gtacgcaatg gggcgatgaa
    26881 gggaaaggaa aaatcacaga ctttctcagt gaaaatgctg aagtgattgc acggtaccaa
    26941 ggtggcgaca atgcagggca cacaattaag tttaacggtg tgacttataa actgcatttg
    27001 attccttctg ggatttttta taaagacaag atcagtgtga tcggaaacgg cgttgtagtc
    27061 aatcctaaat cattggttaa agaattagct tatttacatg aaaaaggtgt agaaacaagt
    27121 aatttgcgta tttctgaccg cgcgcacgta atcttgcctt atcatatcaa attggaccaa
    27181 ttacaagaag atgctaaggg cgaaaataag attggtacaa cgattaaagg aatcggacct
    27241 gcctatatgg ataaagcagc tcgtgtaggt atccgtattg cagacttatt agataaagaa
    27301 atttttgaag aacgtcttcg tttgaatcta gatgacaaaa accgtcaatt tgtaaaaatg
    27361 tttgattcag aaccacttga ttttgacgat atttttgaag aatattatga atacggacaa
    27421 caaatcaaac aatacgtcac agatacttcc gttatcttga atgatgcttt ggatgcaggc
    27481 aaacgcgtgt tgtttgaagg cgcacaaggt gtaatgctgg atatcgacca gggaacttat
    27541 ccttttgtta cttcttctaa cccagtagcc ggtggggtaa caatcggaag cggtgtaggg
    27601 ccttcaaaaa tcaataaagt tgtcggcgtc tgcaaagcct atacgtcacg tgtcggtgat
    27661 ggaccatttc caactgaatt atttgatgaa acaggagaac aaatccgcga agtaggacga
    27721 gaatacggta cgacaactgg tcgtccgcgt cgtgtaggct ggtttgattc tgtagttatg
    27781 cgccattcta aacgagtttc aggtattacc aacctatctt taaactcgat cgatgtatta
    27841 agtggattgg aaacagtaaa aatttgtacg gcttatgaat tagatggcga actaatctac
    27901 cactatccag caagcttgaa agagttgaac cgctgcaaac caatctatga agaattgcca
    27961 ggctggtcag aagacattac aggatgcaaa acacttgcag aactgccaga aaacgcacgt
    28021 aactatgtac gtcgtatctc agagcttgtt ggtgtacgta tctcaacatt ttctgtcgga
    28081 cctgatagaa cacaaacaaa catcttagaa agcgtctggg cgcaaatcta aggataaaaa
    28141 cgacgactga atcaggacgt tcttaccaga gcgagtcgaa gtgtttatct atacgataat
    28201 aataacttga agaggtagtg aaagagacgt ttagccttat tgccgatagg ctgtctcttg
    28261 aacactgttt attcagaata agggactgtg acaaaaa
//
LOCUS       DACDYJ010000033        28099 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDYJ010000033 DACDYJ010000000
VERSION     DACDYJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.033"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(105..686)
                     /locus_tag="IWM51_001486"
     CDS             complement(105..686)
                     /locus_tag="IWM51_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9970912.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(869..1495)
                     /locus_tag="IWM51_001487"
     CDS             complement(869..1495)
                     /locus_tag="IWM51_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9970913.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1517..2848)
                     /locus_tag="IWM51_001488"
     CDS             complement(1517..2848)
                     /locus_tag="IWM51_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP9970914.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(3003..3404)
                     /locus_tag="IWM51_001489"
     CDS             complement(3003..3404)
                     /locus_tag="IWM51_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9970915.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3490..3696)
                     /locus_tag="IWM51_001490"
     CDS             complement(3490..3696)
                     /locus_tag="IWM51_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970916.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3791..5293)
                     /locus_tag="IWM51_001491"
     CDS             complement(3791..5293)
                     /locus_tag="IWM51_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP9970917.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5311..5766)
                     /gene="argR"
                     /locus_tag="IWM51_001492"
     CDS             complement(5311..5766)
                     /gene="argR"
                     /locus_tag="IWM51_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9970918.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5864..6481)
                     /locus_tag="IWM51_001493"
     CDS             complement(5864..6481)
                     /locus_tag="IWM51_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970919.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6702..8075
                     /locus_tag="IWM51_001494"
     CDS             6702..8075
                     /locus_tag="IWM51_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9970920.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8150..9262
                     /locus_tag="IWM51_001495"
     CDS             8150..9262
                     /locus_tag="IWM51_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP9970921.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(9334..10971)
                     /locus_tag="IWM51_001496"
     CDS             complement(9334..10971)
                     /locus_tag="IWM51_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP9970922.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11139..11747)
                     /locus_tag="IWM51_001497"
     CDS             complement(11139..11747)
                     /locus_tag="IWM51_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9970923.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12131..13282)
                     /gene="queG"
                     /locus_tag="IWM51_001498"
     CDS             complement(12131..13282)
                     /gene="queG"
                     /locus_tag="IWM51_001498"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP9970924.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13301..14053)
                     /locus_tag="IWM51_001499"
     CDS             complement(13301..14053)
                     /locus_tag="IWM51_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970925.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14194..15144)
                     /locus_tag="IWM51_001500"
     CDS             complement(14194..15144)
                     /locus_tag="IWM51_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9970926.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15145..15984)
                     /locus_tag="IWM51_001501"
     CDS             complement(15145..15984)
                     /locus_tag="IWM51_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9970927.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15981..16727)
                     /locus_tag="IWM51_001502"
     CDS             complement(15981..16727)
                     /locus_tag="IWM51_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9970928.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16965..18335
                     /locus_tag="IWM51_001503"
     CDS             16965..18335
                     /locus_tag="IWM51_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9970929.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18390..19286)
                     /locus_tag="IWM51_001504"
     CDS             complement(18390..19286)
                     /locus_tag="IWM51_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9970930.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19567..20244)
                     /locus_tag="IWM51_001505"
     CDS             complement(19567..20244)
                     /locus_tag="IWM51_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9970931.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20335..21240)
                     /locus_tag="IWM51_001506"
     CDS             complement(20335..21240)
                     /locus_tag="IWM51_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9970932.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            21415..22869
                     /locus_tag="IWM51_001507"
     CDS             21415..22869
                     /locus_tag="IWM51_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9970933.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            22885..23916
                     /locus_tag="IWM51_001508"
     CDS             22885..23916
                     /locus_tag="IWM51_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9970934.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23923..25110
                     /locus_tag="IWM51_001509"
     CDS             23923..25110
                     /locus_tag="IWM51_001509"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP9970935.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25266..26648
                     /locus_tag="IWM51_001510"
     CDS             25266..26648
                     /locus_tag="IWM51_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9970936.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26620..27006
                     /locus_tag="IWM51_001511"
     CDS             26620..27006
                     /locus_tag="IWM51_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970937.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27206..27874)
                     /locus_tag="IWM51_001512"
     CDS             complement(27206..27874)
                     /locus_tag="IWM51_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP9970938.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         7820 a         5746 c         5166 g         9367 t
ORIGIN      
        1 ttcttcgaaa aataagccga aatttcacaa aaatttgaga agcaattttc gtaaattcct
       61 tcttatttct tgaagctgac cacttctgtc acaacctcta attattaaag aaccttttgc
      121 gaagttaaca atctttcttt gatcaattga aaattctgcc taagtagtga tagatcctgt
      181 gtgactttca acaataagat acttccctgg atttcagcaa tcaacgcttt ggcttgacgg
      241 tctgcttctt catcggacag tttttcttca gaaagtgcca ggatattttg tttcgtatac
      301 ttgacccatt gattgactaa ttctagtagc aacagacggt attcttcatc cactgtactc
      361 aactcaataa tcaaattacc tacaggacag ccatgataag tgatctgtgt ctcatgatca
      421 gcgaaaatcc agtccaacat ttcttctaat gcttcttctg gctgttttcc agcatttaag
      481 atgtcttcga acaattctgt ctgccatacg tccatatgcg catggatcac cgctagacaa
      541 acttcttttt ttgaagaaaa gtaataataa aattgacctt ttcctgtatc agaagctgct
      601 agaatatctc ggattgacgt ttcatgatag ccttttttat aaataagatc tttggtcgct
      661 tccaagatct tttcctttgt tttcaatgat attctccttt ttcatttctc atagtcctat
      721 attatacagt aaattcatca ttttttcttc ttaatcgttt aaaaaagact gtgccaaatt
      781 atctgacaca gccctcacta taaaactgtt ctcatatagc tagatgttca ttgattcaag
      841 aactgcgagg acaaaagcaa ttcctatctt atgatagttg ctctaatact tcctctgttg
      901 tcatcacttg atctgcaata aagccaaagt tcgtcaacgc tgcatccatt gcttcttggc
      961 caggagcacc aattgcatct gtcgctacca caacattgaa tccactctct accaattcac
     1021 gcatatggga atcgctacac aagtttccgt tcattccacc aagaattact gtgtcgattc
     1081 cattttttct caattggagc gccaaatcat tgctttctgg tccaaagatt ttatgaggag
     1141 agctgatgat cgtattttca tccaaataag gtttcaattc ttcgataaaa tcggctcctg
     1201 aaccttcttc cactgattca tattgacttt ttctagcaaa catctgctgc tcaagcatca
     1261 tatgttctcc tgcaccgcca aattgccatt tatggtcatg tgggtaaaaa taatgaggag
     1321 aaataaagag tttgtatcct ttttcttttg cttgttttac catttcaatc atattttcga
     1381 tcgtattgta tttttctaag atatcttttg tcaaaccaaa gccaccgcct gctggtttca
     1441 agaactcgac ttgcgggtca gtgagaacta ctgctgtatt tttattattg aacatggtgg
     1501 attctccatt cgttatttat tttaatacat catttagagc ttcactcatt gtcggatgag
     1561 aatagatttg atctctcaat aacgtataag gaagttttcc attcatagct agcgaaatca
     1621 agttgatgac ttcatgagaa tcttccgcat agatacttgc ccctagaatc aagtcagttt
     1681 cagggtcgac taaaactttg aatacacctt taggatcttc taatacttgt gctttaggga
     1741 caccgcttgt catatacttg aataatttgt aattgacgcc ttgtgctttg gcttgttttt
     1801 ctgttaaacc tacattggat aaagtcggag tgataaatac actgtaaggc acagctttcc
     1861 ggttattcgt tgttcgttta ttctcgccct tcaactgatc cataatgatt cgataatcat
     1921 ctagagagat ataagtgaac tgcaatccgc ctttgacgtc tccaatcgcc caaatgttgt
     1981 ctgcatctgt acgaaggtat tcatccacag cgattgctcc gcgatctgtt agtttaacat
     2041 ctgtattttc taatccaagt gatgctgtac ttggttttct tccagttgca gccaaaactt
     2101 ttgacgcttg gattgttact tcttggccat cttctgtaaa ggtgacggca gctgttgatt
     2161 cttggtctgc tacttgatct acagatacac ctagatggaa ttcgattccc gcatttgtca
     2221 tgtcttctaa aatcatttgc gcgatatctt catcttcacg tggaagaaat tcagaatggg
     2281 catcaagaac gacaacttta gatccgtatt cattgaacat tgatgcaaat tccaagccaa
     2341 tatatccagc cccgatgatc actaattttt ccggtaattg ttcctgatcc atcgcttgcg
     2401 tagaatcaat caagtagcga cttgtattca atcctttgat tggtaaaatg attggttcag
     2461 aaccagtatt gatgaaaatc cgttcgcctt caacaatcac tttttcacca ttattcgtca
     2521 cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc catcactgtc ccagttgcct
     2581 catcgctgat catatgataa tttttggcgc gaagcatgcc cgtcaatttt tctttccgag
     2641 caacagcctc tgtaaagtcg attcctcttt ctccattgaa aatcaatgat ttagaaggaa
     2701 tacagccgat gttgatacat gttccgccat acatacgtgt tgacttttca acgactaaaa
     2761 ccgattcacc ttttgaagct aacgccttcg ctaaagtctt accgcccttt ccaaaaccga
     2821 tcacaatatt ttgatatttt tccatcattt ttccctcgct tcttttttac ctaccgattg
     2881 gtacaaaaat atgataaccg ataattgtct attctccaaa tcatctgctt tatttgtcaa
     2941 aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc tggttcgatt tcctttattc
     3001 ttctaaacaa tcgttacttc tacatcgata tttcctctag ttgctttgga gtaagggcag
     3061 aattcatgcg catcttctgc aattttttgg gcttcttctt cgctgatgcc atcgatatgc
     3121 acttctaata ctacaccgat ctgatagccg ccatcatcta aattataaag agagacacgt
     3181 gcttttactg tggactctgc tgtcattcct gcttctttga tcatatgttg taccgcccca
     3241 ttgaaacaag aagcataacc tgctgcaaat aattgttctg gatttgttcc ttctgcatgg
     3301 attcctggag gagtgatttt catatgcatc gagccatttg gtgcctcgac ttctccattg
     3361 cgcccgccat ggttgatcat tgctgtttcg tatgcttttt tcattgtact gctcctttcg
     3421 attgaccatt tgctacttgg ctataattat agtgtatgac aagtaaggcg ctttctacaa
     3481 aaattctgct tattcgaata ccagaatgcc gtcttctact atttttccaa aaaacaaaaa
     3541 aatactactc gtgaacaaca gcgtcatatt tgttccaaaa taaatagcaa tcttttctac
     3601 ataatctttt atatgattga ttttaagata catatatacc agaaagttga ccccataaac
     3661 caatactgtt aaaaaaataa ccatattcgt taccattttt tcctcctttt gctggtctcc
     3721 ctaccagcac tcgatttaac cataccactt tttttgccgt ccaagcaact agcctttcaa
     3781 aaagactggt ttattctgct gttaatgatt caagatcaga aaaatgtccg aataattttg
     3841 ctaattttac taacgccaac ttgttctttc cgaattcatc attttctatc tgtaatacta
     3901 gtaacggctc atgtaagctt aaacggatca aaaaccagcc gtttccgtat ggctcttcta
     3961 gatcatatcg aataccttct tcattttccg ggtcgaagtt catccctttt tcttcttcca
     4021 acagtcgctt catttcttct aatatcttgt ttccttgttc gcgtggttca tcgctgaaca
     4081 attttaatcg gatttcttgt acttctacgg gctgtttcaa ctctttgatt tgttcatcta
     4141 atgtgatacc ttttcttttt aattctggca aaagcatcag gatctttgcg atgacatatg
     4201 caccatcatc cagaaaatag ttttccttga atgcagcgtg tccgcttgtt tcgatggcta
     4261 gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt attgattaca tttcgatagc
     4321 cggaaatata acgaacttgg atgcctcctt tttcttcaat gaatctttgt agatgagaag
     4381 aagtagggga attcgtcacg attcttgttc ctggatgctc ttttaatacg attgtactta
     4441 atactgcgat caaattattt cgattgatca ctgttccaga agcactgaca actgcagaac
     4501 gatccacatc tgtatcgaaa atgatcccaa gatctgcttc atttgctaaa acagcctgct
     4561 tgatactttc catcgcttct ttattatcag gatttgggat atgattagga aatcggccat
     4621 ctggttccaa aaattgggaa cctgttgaat ccgcacctaa gctattcaat actttttcag
     4681 caaagaatcc accagcacca tttccggcat caacaatgat tttccaacca tttagtggtt
     4741 tgctttctag tgttccaatc ccctttttaa tttttgtgac taaatcttgc gcataaggtg
     4801 tcaaaaggtc tgctttttct acgcttccag tttgttcgct ttttattttt tgctgctgtg
     4861 ataggataaa agcaatatct tctttttctg ctcctccttc tgaggtaaag aattttattc
     4921 cattataata atagggaaga tggctagcag tcagcataac cgcagcatcg cacgagaact
     4981 gttcaaattg agtggacata aacatagcag gcgtagtagc taattcgaaa tcgatgacat
     5041 gtatcccttg ttctttcaga ctttggatca acgcctcttt cagtgctggt ccgcttatcc
     5101 tactgtcatg accaatccca atcgtccatt ttttatttgg atcggttgat ttcttctttt
     5161 tctttatcca gtttgctagt ccccagccga tcttttttac catgtctgtc gtcaagttta
     5221 cttcatatcc ttcaactggt aatgcgattc ctcgaatatc cgatccattt tgcagcgctt
     5281 tcatatcttt catataaaag tcctcctcat ttattccctc attttttcca gcttttctgc
     5341 aaatcgctgt gcttcttctt ctgagcggca aataataatg aatgtatcta caccggccat
     5401 cgttccgaca acttcagggt aagccacttc atctagccaa ttcgttacca catcggcacc
     5461 atttcgttca gtcaatacga taaccatgaa ctgaacagac tggatacgca aaacctcgcg
     5521 tctaattgct gtacgcaaac gttcttcatt taaagaaaca gatggctggt taaataatgt
     5581 atagcgtact attttgtttt cattttgact ttttactaac ttcatttctc gaatgtctcg
     5641 agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc agccgcgcaa gcagctcttc
     5701 ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc agtgcttgtc gctgtttctt
     5761 tttcatatta gacacctctc aatgttgatt atagcaaaag ataagtaata tttcttcata
     5821 ttttggtatt ttttactttt attttttgtt ttcattcagg ttgctaacct ttttctattt
     5881 taacacgcaa aatcgtcttc attttttcag gtgctgattt tcttggatcg ctgatgtaaa
     5941 tctctttgtg ttctttagaa gctctttcat agccttcttt tgcgatgaat gcttctaatt
     6001 tctcaaacgt tgctggttct tcggcatagg gcccgacgtg taagatttgg gcacaaagac
     6061 cttcttccat ctttttgaat actagttgtt ctttcaagtc ttccggaagt ttgctagaac
     6121 gctgaatggc ttcttgtgct atcgcttctg taacaaagtc aggctgtcgg atcatcaatt
     6181 gataagaaaa atgttctttt ttcatcactg gttcatgcaa atacttttct tgcagtcccc
     6241 attgtccttc cagtggatag acagtataca tctgataatc aggaatcagc caattttttt
     6301 taggcgacat gcgtattgca tagctgactg catatagaca ggctactctt cgtttaaagt
     6361 cttcctgatt cgggtcgccg atccctctga tgctaaagaa tggtttttta ggaacctcta
     6421 gtatttgcgg gctttttttc atactatagc tcttgtcttt acgccattca attttttcca
     6481 tttttcttcc tccgcgaaat tccatttagt tatttattat catacctctt tttgagtgac
     6541 ttggctaatc gtgttgacac tatttttcag tttgtgctag aataagtgaa gtaaataaat
     6601 cgtacagata acacgtttcg gcgtgtcgag agagaggcgg tttctatttt tataatagaa
     6661 atcactctct ttttttacga aattttttag aggtgatgta aatgacaaaa caaaaacagc
     6721 tgcttttagt acctgcagta attgcaacag ggatgatgtc ttttgcgggc gtattgatcg
     6781 agacggccat gaatgtaact ttcccaactc ttatcagaca gtttgattta acgactgcac
     6841 aagtccaatg ggtaacaacg atttatcttt tgatgatttc gatcattgtt ccgctgtcta
     6901 cttatttgaa tcgcaatttt agtctcaaaa aactcttttt agcttcaaat ctgcttttct
     6961 tggcaggtat aatgatcgat ttcttctctc ctaccttcat ggtcctattg tttggtcgat
     7021 tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat gttccatatt atcttgacat
     7081 ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg tactttgaca acggccattg
     7141 ctccagcaat tggccccact tatggaggca ttatgacttc acattttaca tggaatcata
     7201 tcttcctgtt tcttgtgccc gttttgattc tttctttgat tatcggctgg tttgctattc
     7261 ccgagatggc agtacaaaaa agtggttatt tggattggat aagcgtttta ggaatttgtc
     7321 ttttgtttag tggaagtcta acatttttga gtacgttggg aacagtttcc agctggattg
     7381 ctttaatcat cggcggtatt ggctggttcc ttttttacta tcgaagtaaa aaagtggagc
     7441 atccactgat ccgactgtct attctttcaa ataaaacatt ccgcttgttt ttgattggct
     7501 tcttagttta tcagttttta ttacttggtg tatcttttgt cttgccgaat ttccttcaga
     7561 tcgttcaagg aagtagtcct tttgtcgctg gattagcgat gcttcccggc gcagcggttg
     7621 gtgctttgct ttctcctttt tccgggaaaa tgctggatca atatggtccg aagaagccaa
     7681 tttttgctgg gctgattctt tctttgattg gttggctagc attattccta ctcataggac
     7741 acgcttcagt gggtgtattg attgcttgtc atgtgtttta tatgatcggt atcggattat
     7801 cttacagcaa tatgatgacc accggaatga atgcactgaa tgaagaacta caaggagacg
     7861 ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc tgtggctact tcattagtag
     7921 cggtcattat caattacgtc cagcatcata cttcccataa ctatgaagtg tctactacat
     7981 taggatcaaa agcagcatta ggcgtcttgt tgctgttgct tctcgtaagc tttgctcgat
     8041 tcgcctatta tctgttcttt gctaaaaaag cgtagattcc atatacggca ggtgttgcat
     8101 ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga ggctttttta tggaaaaaat
     8161 tgccattatc ggtggcggga tcattggtat gaccctagcc agttacttag atacatcaaa
     8221 attcaatgtt gctttatttg atgaagggac aggacaggca actaaagcta gcgctgggat
     8281 tatctcccct tggttgtcga aacgcagaaa caaacaatgg tatcgtctgg ctaaagacgg
     8341 cgctgctttt ttccctaaac ttataaaaga tttccaactt gatcaaacga tttacgcaca
     8401 atcagggaca cttttgcttc gacctgaaaa agatttagct gaattggctg ttcttgccga
     8461 gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa tggctttcac ctgaacagac
     8521 atctcaagca cttccgttat tgaaaccagc agtttcactg cgtatcagtg gtggtggtcg
     8581 tctggatgga aaagcatatt tagaacatct tggtcagcgt ttagcacaaa aacaaatgga
     8641 gcagatccag gcaaaagtca ctttgcataa gaagagcgat tcttggctca tcaagggacc
     8701 gtttggagaa tacacagctg atcgtgtaat tttgacacct ggcccggcac ttaagccttt
     8761 attacaagaa ttaggctatg ctgcagatat acgtccgcaa aaagggcagc tcttagcatt
     8821 tcagacatct tataagatga gtcaagactg gcctgttgct atgcttgacg gagaagctga
     8881 tttcattcct ttcatggatg gaaagatcct tttaggtgct acccatgaaa acgatcaagg
     8941 ctgggatttg gctcctacta gcgaagctta taagggattg actaagagta cagaacagtt
     9001 cttgactgat ccagatcaga taaaaaactg gccatacgag ttccgtgtag gtacccgtgc
     9061 ttatacgtca gatttttccc catttttcgg cgtactgcca gatgatccaa gcttactggc
     9121 tgctagcggt ctaggctctt ctggcttgac cacaggtcct tatatcggct atctattagc
     9181 caacttctta aatactggtc attggtctac ggaagattat caaaaggaca tctccaccta
     9241 tattacgaaa aatccatcat agaaaaacag ctttttcaag taaggatccc gagcgatatt
     9301 gcgcatggat tacttcttga aaaagctgtt attttagttc aatttctgaa tcaaattctc
     9361 caaactttct tttgcttcat ctggcaagag atcttttcct atctgagctt caatgaatcg
     9421 taaacgaata tcaagtcgtt gtttcaccag atttttatag gacgtatcct caaaagaaac
     9481 aggaaaatct ggtataggta aatagtggat gtcttcaaat ggaccaaacg acttgaattc
     9541 tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc gtttcaggtt tgattttttt
     9601 atcatcgaaa tatccagtat tcaggacata gcaggctgtt tgctgtttca aaaacaactc
     9661 tcgaaaagct tgatagtctt ccctaagagg atagacacgg aaaggattag caaatggttc
     9721 aatgactagc tcatctagct gtctttttgt agtgatattt tccgcgtctg atcgttttgt
     9781 tgctaatgtt gcaccaaaag acgctgctaa aactgggtca ttgatcttga taattggcgg
     9841 aagcgaatca tctttcatga tccaaaaaac agcatcgatc ttttcgctaa gatgatcgac
     9901 tcggtcgggt gtcgcataac gtgatttgac tgtccgccca tttccgtttc gtatatctcc
     9961 tgttactagg actttttctc ctttacttgt acatgtcacg cccacattct gacaagtaag
    10021 gaaagaagaa atggccggat catcagacgg atagtcttgt gttttatcaa agtaagaagg
    10081 ttccaaggca gtagcaaaac cattttttcg agaaataacg aaagcatcgt catgcaagat
    10141 cgaaacagag tgaccttccg tatgttttgc taaggtaatc gttgattttc cagaaccgga
    10201 caacccaaat acagccatcg tataggtctg gtcattacta gcatattgtt tcatcccacc
    10261 gtggcaggca ataaagccat tgcgatttcc caatgcccaa gcaagcgtca aagtcgcttt
    10321 ttttaattct ccaaagtaac gtaatcctag cacagccgct gtattatgga gagcatcaaa
    10381 caaaatgagt ccatttggaa atgtttcatg tgtccattcg ggatcacaaa agatataaag
    10441 atctccctca tcaaaggaaa cagacttttg ataagtctct aagatcttcg aattcgctgg
    10501 ctgaaaattc agcaaataag aatacaaatt gttctcaaat ccttctggaa tcagcaaatg
    10561 actttgcaca gaaaattctt cagataggcc aacgattact tttgtatggt aaaactggcg
    10621 cttgcgactt tgatacacag cttctcttaa aattttctcg tatttgcttt gttccttgtc
    10681 taagtcctca aagatccgcc gagcacttgc tgttcttccc acaatgcttc catcattgaa
    10741 tactagtgtc ctaacatcag ctggtaaatc tagttcttca gcatgtgaga ttgttaaatc
    10801 tgtgatgatc gttcctggac tcccttttgc tagttgataa gctgtcttcc tgtcaaaaac
    10861 ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc gatcgaatac tggataacag
    10921 tggatttcca cttgtgattt cgtttaattc gagactctct tttgtactca tggctcctcc
    10981 tcattcccta aacaaaaaaa cgttttcttt attatacatt attcgagttt ttatttgtac
    11041 tgctgcgctg tgggaagcaa acgagttatt tttcacacaa aaaaaccgag aacttgaata
    11101 aacaagtttc tcggtttcat cattttcctt ttatcggctt agtaccagcc gtttgctaac
    11161 cagaatgttt tagcagcatc ccatgaacca taacgagatg ttacatattg ttctgctaca
    11221 cgttcttgat tggcagctga atagtcgccg tttaagtatg aagcatctaa ttgataacgg
    11281 ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg attctttttg tgcgatccat
    11341 tcttttgcag agcttgaagt tggcgctgtt gcagcagctg gggcagcagg agctacttca
    11401 actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg ctgtttcagt tgtagatgct
    11461 gtattttctg ttgttttgtt ttctgttgtt gctgaagaat catttgaagt tgggattgtt
    11521 aattgttcac caacataaat gcggttgatg tttgcgatcg aatttttttc agcaattgca
    11581 tcgatcattg agttgtctcc tgcaaatttt tgtgagatct tagataatga atcacctgat
    11641 tttactgtat aaacttcatc agcgtgagca tttgtagttc ctacaaacat tgcagtagca
    11701 ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg aattcatatt aaactccttt
    11761 ttttctccgt tttttattct ttcgacgcta ttaactttat catttagaaa tatttcatag
    11821 gtaaaagaaa catgacattt aatgacaaaa taacgaatta tttaatattt gtaacattta
    11881 aacaaatatt tttttgatac aaaaccataa aagccttgtt gtataagcaa agtaatcgtt
    11941 tttctatgtt tttttatttt tgaatgaaaa acgcagattc tgtaacattc actgcaataa
    12001 tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa ttctcttaaa aaaatgtaaa
    12061 aaatacaaaa aaaccagtaa ttgttacaac aattactgga caaaaacttt gaataatgtt
    12121 aactattttt ttattttaac tttctccgca ataatttctg ggcatgttcc aattcgttta
    12181 taacgacttc ttctgtttct ttagctaata tttcatctaa tacatcaagc catttttccg
    12241 gttcttttgt tcctagacgt ccaatagccc aagctgctgt tcctctgatg acagggcgga
    12301 catctgttaa acattctgaa atctcaggca atgccgttcg atcacctaga ttagctaaag
    12361 caatcaatgc gtttctctgc aaaggtttct tcccacgcca tgaaccagct aaatgcccaa
    12421 attgctgttt gaagtcttta ttagacatcg aaagcattgg ctttaatttt ggatatactt
    12481 cgtctacttt tggttccatc tcttcatgaa aatgaaaatc ttttccttga ttgtaagggc
    12541 agactaactg gcagatatca catccataaa tgacatttcg cattttttta cgatattctt
    12601 ccggcatcat cccttttgtc tgagtttgat aagacaaaca tcgctgggca ttcattcttc
    12661 cgtcaccaag caatgctttt gtcggacaag cagtcacgca gcgcatacag tcaccgcaac
    12721 caaattctcc tggtttatca ggctcaaata caatattagt aacgatctca ccaagataga
    12781 caaatgaacc gaactcttca gtgatcaaca atccgtttcg tccaataaat cctaaaccag
    12841 ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc tacttgcgga gctagacgcc
    12901 attcctcttc tttttgccat ttttctgctc ttgtttggat gaattcgatc aatcggttca
    12961 aacggtctct gagtatatca tggtaatcga tcccccaaga agcacgagca aactgcccgc
    13021 gtttttcatc cctaggcact tcttcatgga tcctagtagg atacgcaagt gcaatggaaa
    13081 tgatcgattt cggctgatca aatgtcattt ctggataaag acgctcatcg atatttggat
    13141 gttcaaagcc agaagtatgg ccagctgctt tttgttcttc caacgattct tttaatgaat
    13201 caaaaggttc cgccgtggtg aacccgattt tgtcaattcc taatcgtttg ctttcttgta
    13261 tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg ttaacggctg tataacccat
    13321 aatatagttt ttcttttttc agctggacaa actggatctg ctgatcggca atcttctttt
    13381 cttccatcca tttttttctt gcagcaaata cttcccaata atttgttgaa attaattttc
    13441 ttttgttatc taatgcatta aaaaaattca atgaaactgg ttttgtactt aacagttcat
    13501 ggagccttac ctcttcccat ggcgaaaact cagacagatc gcgggaatac aatacttctt
    13561 catagagatt tttttgctgt gtgtacgtga atgtttgcgg gattttccca ggcctagcaa
    13621 tttctactag acgattataa aacatattca ttaatttgct gttggcaata aattgacttt
    13681 tataataggc cgactggatc tttttgatta ctccttctgc tttgatgact gaaaataatg
    13741 caggccatgc tttgacggtt atttcgtcgt tttgtttaac taatacgcca ggtgtgaagc
    13801 cgactggaga aaggaacaca aaataaccga taccggtatt ttcatctaaa ggctgaaatt
    13861 catcaaacaa ccatttgcct atgtacagct cttcatctgg ttctgttgtc tcattttgat
    13921 ttgctatgaa taatttagct aattctgctt caagaaattg aaggtcttga tccttgtcgg
    13981 tattttcctg ctggctgatc aatcgaaaga cttcttcttt aatcgtatat ttatctttac
    14041 gtgtaaaatt catagccaca ccttccttcc ttactctaac tataacggaa tttcttcaaa
    14101 aaaggtatct gtacgacgaa aaacagcata attttttttc atatctctta taaaaaatgc
    14161 aaggtctgct cctcaagcta gaccttgcac ttttcagctt tcttttgttt gtgacaaacc
    14221 ttcatggatc gtttcaatat tccatttcat catctgatag taagaatctc cttgttctcc
    14281 ttcttttgct atggaatcag tgaacagttt atcatagatt ttcaaacccg tttcttttgc
    14341 tacccgttcc atgcttcttg agtctacact ggtttccacg aataaaaccg gtacttcttt
    14401 tgctcttatc tgatcaatga tcgctttcat ttgatcaggc gtaccttgac tttctgtatt
    14461 gatctcccat atataagctg ctggcaaatc atatgctcgt gaaaaatact tgaatgcccc
    14521 ttcacttgtt actaataatt ttttgttctc ttcgatagaa gcaaaacttt ccttcgcttg
    14581 tgtatctaat tcttttagtt tttccacata tgcttctgca ttttttttat agagtgtcgc
    14641 attttctgca tctttttcag agaatatacg tgcgatttcc tccacatatt ttattccgtt
    14701 agataggtct agccaagcat ggggatctgc ttttgtatgt tcttcaccgc tagttaaata
    14761 aagaggttct acatttttgc taactgcaaa ataatctttt ccttcttctt ttttagccgt
    14821 ttccatcaag ttatcgaacc agctgttacc tgtttcaaga ttcaaaccgt tgtataaaat
    14881 aacatctgca tcgcttgcct ttttgatgtc ttctggtagt acttcatatt catgcggatc
    14941 tgttccgact ggtacgatac tgtggatatc tatttgatct gttccgacct cttttgccat
    15001 gtccgccaag atcgaattgg tagccacgac tgacaacttt tctttacttc cttcatccgc
    15061 ttggtcagaa gcctgctttc cgcaaccggc taataccaag aaactaactg ccaagattaa
    15121 aaagagtgat ttttttgttt tcatctatct gcttcctttc tgaaataaaa ttccttttgt
    15181 tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat gctaacacga tcgtagctcc
    15241 tgatggccaa ttgtaactat aactgaaaaa cacaccgaca actgcactta gcataccgat
    15301 tcctgcagct gtaatgatca ttttcagtaa atggttcgtt agcaagtaag ccgtggcggc
    15361 tggtgtgatc aacatggcaa tcaccaagat tgttcctact gtctgtaaac tgactacagc
    15421 cactaatgtc aaaaagaaca tcaaaagata atggatcagc gatgtgttca gcccataagc
    15481 ttttgccatt gtcggatcga atgatgtgat cttcaattct ttataaaaga aaaagacaaa
    15541 tatcaagacg attccactaa cgagtgctgt cagcaacaaa tcgctttccc taacagctaa
    15601 gacattccca aataaaatat gatacaaatc tgtcgcactc tgggcatatg aaatcaagat
    15661 aatccctaat gcaaaaaagg agctaaagac aattccaatc gctgtatcat ttttcaatcg
    15721 gctccgctga gtcacgaaac caatcactgc tgctgttagc atcccaaaag cagtggctcc
    15781 aaagatgtaa ctaaaaccaa acatataaga aatggctaca cctggaagaa ctgcgtgaga
    15841 gatagcgtct cccatcaaag acatccctcg taaaataata aaagaaccga tgactccaga
    15901 agcaagtcct acaagcatgg aagtgaccaa agcgtattgc aggaattgat aattcattaa
    15961 tccatcaata aaggattgga tcattttttc tctcctcctt ttactaaatg tctgatgatc
    16021 tcaccatagg ccaattgaag gttcttcgat gtaaagactt cttgtactgg accggaagcg
    16081 atcagctgtt tatttaaaag aataacctca tcgaaatatt cttctacttt atgaagatca
    16141 tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt cctgcaagat gtcaaagatc
    16201 tttcgttcac ttaacgcatc aatccctacg aatggttcat ctaaaaagat ccattctgct
    16261 ccttgggcta gagctcttgc aataaaaact ctctgaagct gtccacccga tagttcgctg
    16321 atctgtcttt tggcatattc ttccatccct acttttttca aagcttgtct tgcacgttct
    16381 ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg gatatgttcc taaaagtaca
    16441 acgcctaata catcaattgg aaaggaaaga tccaattcac ttcgctgttc cacgtaggct
    16501 atttttttac ggactgagtt taccggctgt ccagaaaagg tgactttacc agtagtagct
    16561 ggtatcaact ctagcaaaga tttcataaat gttgattttc ctgcaccgtt tggtccgata
    16621 ataccggtaa ttttttgtgg atttatcgtc aagttgatag ttttcaaaac agttttccct
    16681 tgataagcgg ctgaaaggtc ttgaattaca atggccgtac gttccaaatc ttacactcct
    16741 ttttaggttt acctaacttt ttatttaaat atagtttaac taaaatggaa agcattttca
    16801 agtagaatga tgaaaaagtt tttttcgaat tttcctcgca ttttatgaaa attttctgta
    16861 gaatcacatc aagagttcaa taaggctaac ctaataatga aaaacatgtg ttgaaacgtt
    16921 tgattatttt ttcttgttga ctcaacaacg aaaggagttt ttgtatggaa gaagaaaagt
    16981 taactcgaag tcttagtgcc cgtcatatcc aaatgatcgc actaggtgga acgatcggtg
    17041 tcggtctgtt tatgggtgcc tcctcaacca tacgttggac agggccctca gtgatgggag
    17101 cctatgcgat tgcaggattc tttttgtatt tgattatgcg tgctttaggc gaaatgcttt
    17161 acgttgatcc ttcgactggt tcttttgcaa attacgcgag cgagtacatc catcctgtgg
    17221 ctggctactt gaccgcttgg agtaatattt ttcaatatat tgtcgtagga ataagtgaag
    17281 tgattgctgt aggatcttat atgaattatt ggtggcctga tttgccggca attattcccg
    17341 gaatcattgt cgtcttgttt cttatgctgg caaatcttat ttctgtcaag gcgtttggcg
    17401 aattagaatt ttggttttcg atcatcaaag tcataacgat cattttgatg atcattgcag
    17461 gtcttggggt aatcttattt ggatttggta atcatggtca ggcagtaggg atttcaaatc
    17521 tttggaaaaa tggtggattc ttcactggag gcgtcaaagg atttttcttt gcattatcga
    17581 tcgttgttgc ttcctatcaa ggaatcgaac tgatcggaat gactgctggt gaagcagaga
    17641 atcctaaaaa gacgatcatt gaagctgtcc agtccactat cggtcgaatc ttgatttttt
    17701 atattggtgc tatttttatc attgtgagta tctatccgtg gaatcaattg agtgagattg
    17761 gttcaccttt tgtacaaact ttttctaaag tcggcattac atttgctgca ggactgatta
    17821 attttgtcgt gattacagcg gcattatcag gttgcaattc agggattttc agcgcaagtc
    17881 gtatggtcta tacacttgcc ataaatggaa agatgtctaa aaaatttctg aaactatctc
    17941 gtcacggtgc gcctttttat cctgtcgtag caatctcagg tggaatctta attggtctga
    18001 tcttaaatta tcttctacca atgttcatca accagtcaga aaatattttc gttttcgtat
    18061 acagctcaag tattttgcct ggtatggttc cttgggtagt gattttgatc agcgagattc
    18121 gcttcaaaaa gaagcacgca gataaaatgc atgatcatcc ttttaaaatg ccactatctc
    18181 cttatacgaa ttatgtgaca cttgcttttt tagctatcgt attgattttc atgtttatca
    18241 atcctgaaac gagaatctca ttgattattg gactcgtttt cttgctttac atgacgatcc
    18301 attactttgt acgcgaaaaa agaaccggaa aataaaaaaa gaacgaagct tcaccttagt
    18361 ggaagcttcg ttctttttta ttgcttttat caattcaatt ttagcgtctc accgggagtc
    18421 aggaaataat tctccatgct catcccattc agcgacaaga tcgtttctac tggtacgcca
    18481 gttcgttcgg ctatttgatt tggaccttct ccttccaaaa ctacggttgt cgcagcctgt
    18541 gaagtcgcgc tgttggtatt agtttcagag gaatctgttg tgtcttgtgt tgttcgagct
    18601 tgcatttcag aagcttctac cgactctgct gtctcagaat cagaagatgt tgtgtcttgt
    18661 gtgcttgttg actctgcttg aagagaggcg ctagtctgca aataaatatc ggaaatctta
    18721 gcgacatgat tcgtataatt agccgataat tgattgatca aagtatctaa gtctacaccg
    18781 tctttatttt ctgctgcctg atacgctgca cgtaattttc tgatcccttc gtcggagatt
    18841 gcttgaagct cacgtgcttt ttggttagca actgctgata aaccagctat tccctcttga
    18901 tttccctgaa tttctgtctg gtattcttca atcaatgcag gcgtcttttc ttcgatcttt
    18961 ttcgaataat catttacgat tgcttgaagt tgctctggca aaccatttgc tgttttttct
    19021 tcagaagttt gtgtctcttt tgttttagtt gatgactcac ttgtttgttt cgtctctgtt
    19081 ttctttgtcg ttgtttccgt tatggtcgta cttttcgctt ctgacgaatt ttttccatga
    19141 aaatatagac ccgcaactcc tcctgctatg atcaaaaaca cggcaactgc tgccccaatc
    19201 atgatcatcc gcttacgagc tgcctgtttt tgctgtcttc ttcgggtcat ccgaacattt
    19261 tctccccact cattttcatc atacattttt ttcaccacca atttttatgt ataatagcgt
    19321 tatcaaacgc ttcatattct tattgtagca aataattgtc gctgtgcaca tttttatctt
    19381 tgttatttat aagaaaattt caccagttga gatgattcca agattgtccg tcgatttctc
    19441 cttgcagctg cattgttcgt tcgtctatct cagccatcag tttctttatt tcatataaga
    19501 gcgcacggtc ttgatattct gatttttcgc ttaattcatc taattgttgg atcagccaat
    19561 catttgtcat aatgaagttc ctttcatttc ttgcagtgta aaggtcaagt cttcgatctg
    19621 ctcttttgcc tcagtcagtg cttctacgat atatgcttgt ttttcagatt cttcttcctg
    19681 cattttctga ttttcctgta ctctttgaat aaaccagtca atccgctgag tcagcgatgg
    19741 tttgccttcc aatgagagat actgctgata agcaattagc ttctcccaac gtttttcctg
    19801 ggataaatac ataaattgtg ctaaaacgaa tggcggaaga taggtcatat ggaatttatt
    19861 tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta tattcttctt ttcctccagc
    19921 ttggtattct ttttccgcca cacctgttgt caatacgatt cctagttctt tcccttttaa
    19981 tacatgtgct gctcgttcca agacattatc ttgccaaatt tttaaatgag caggagaact
    20041 ataccagtaa aacggaaact ggaaaatgat ccgatcatgc tgggccaaga gctgtagttc
    20101 agtctctata tctatttttc cgtctggata acttgattcc aaatgatgcc aagtagctgg
    20161 taaaccagca gaagcttctt tgaaaaattg ctgatttaaa gattcttcga tcgttggatg
    20221 gctaatgatg ataagtgtac gcatcccttt tcctcctttt tagtcttcgc tttacagtgt
    20281 agcataaccg ccacaagttt cttttgccga tgcttgtcat tttaaccaga ctaatcaggt
    20341 tgtccaagaa actgcggttt gtttttccaa ctgttttttc aaggtatcca aagcaatatt
    20401 cttaaaattt tcgtttttca tacgtttacg atagttcgta gccgtttctc cggtactctt
    20461 tttgaacatt cggctaaata cgactagact attgaaccct acttgatagg caatactttc
    20521 cacggattca tccgtcagag aaagaagaaa cgcccctcga ttgatccgta ccccgatcaa
    20581 atattccttg atactggtac ccatatgatt tttgaatact cgcgacaaat ggctccgatt
    20641 gaccgctaaa tgctgggcga tttcctcaac agacaatttt tcagcataat gtgcctcgat
    20701 gtatttgact gtttcaacag ctaaagagga atgaaccttt tgtacaccac cgctgacctt
    20761 tccgccatct tttaaaagga ctgataaaaa acggtaggtt aatgctgtta actgtaattc
    20821 attttccaag gtttcttccg tatctttcat gcattctaaa ataatgtcga tatatttttg
    20881 tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa agcgactgcc gaatgatttc
    20941 ttctgcagca ctcccaccaa atgaaagcca gcaatacatc cacggatctt ctccatctga
    21001 taagtaaaac gtcgagtctc ctggacgaat caagaacaga tcaccttttt tcaattggaa
    21061 ttgttgattt ttcacatgat aagatccttt gccttccatc acgacatgca ggatgtaatc
    21121 attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt ttagagaaac cacaaaaatc
    21181 aaaatacaat tctttttcta cttgttgatg ttcctttttt aaaaaaatcg attgctccac
    21241 gtctatccct ccttactcct ttagtataat gcaacaaaaa gttaaataaa agtcacagaa
    21301 atatattaaa aaggaagcga tttcttgtta agtttaagtc attaaaagat ctttgttatt
    21361 cgttgtaacc ggtttactta gtattatata ttgaacagga gaggaataca gattatgaaa
    21421 agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt ttggacacga cacattctat
    21481 gttacattaa gcacttattt tatggtattc gtgacaagcc agatgttcgc aggaacagat
    21541 aaggcaacga atgcaaaaat gattgctaca gttacaagct tagttgtagg tatccgctta
    21601 gttgaaatta tttttgatcc tttgattggt ggcatcatcg ataatactcg tacaaaatac
    21661 ggtaaattca aaccatggtt agttatcggc ggcgttgtta gttccgtact cttggtagca
    21721 atctttacca atttctttgg cttagctact cataatacga ctttatttat catattattt
    21781 gcaatcgttt tcatcttatt ggatagtttt tattcattta aggatatcgc tttttggtca
    21841 atgattccag ctttgagtac ggaaacaaag gaacgtgaaa aatttgctac gtttgctcgt
    21901 ttcggctctt ctctaggtgc aaatggtact actctagtcg ttgtccctat cgttactttc
    21961 tttacctatc ttgttactca taaacatatt gaaggcgctt caggctggtt ctggttcggt
    22021 gtgattgctg ctgttatttc aggcggtact gcgattatca ctgccttggg cacaaaagaa
    22081 gtcgatactg cgatccgtca gcaaaatgaa aaagctggga tcaaagatgt gttcaaagca
    22141 gttgctcaaa atgatcagtt gatgtggctt gcccttgctt atatcgctta tgcgattgcc
    22201 aatgtagcaa caactgccgt attattctac ttcttcaaat atgtacttgg ccaaccaact
    22261 gaattttgga tcgttggtct agttgctgcc ttacttggtt tagtagctgt tccactattc
    22321 ccaatgctta cacgcgtcat ctctcgtcgc tacgtttacc ttggagggat cgcaatgatg
    22381 atttcttctt actttttctt tacgatcgca ggttcaagtt tgccaatcgt tatcatcggg
    22441 ctagtattgt tctacttccc tcaacaattg attttcttat ccgctttgat gacgatcaca
    22501 gactctgttg aatatggtca atggaaaaat ggtatccgta acgaagctgt tactctatca
    22561 attcgccctt tactagataa aatcgctggt gctttgtcta acgggatcgt agggtttgtc
    22621 gctatcgctt ctgggatgac tggtaatgct actgctgctg atatcactgc tcatggcgta
    22681 acgactttca aagcttatgc attctatgct cctgctgctt tgatgatttt ctccgctatt
    22741 atttttgctt ggaagattac attgacagag aaaaaacatg cacaaatcgt tgaggaattg
    22801 gaaaacaaat tggctccagc tgatacaatg gaagatgaac ttcacgatgc agtagttcct
    22861 tcccattaag aaaggatctt gaaaatgaag attacagaaa aagattttgg ccaaggttat
    22921 cacttgataa ccttggagaa caaaaacaag ctagcacttt ctatctctga tttaggtgct
    22981 agaatcgtca gtttaaaatc aaatgatcga gaattagttt tgggatttga caccgctgaa
    23041 gaatatatcg aaaaagatcc gtatatcggt gcttctatcg gtcgcaccgc agggagaatc
    23101 gaaaatggcc gcttttctct caatggaaaa acttaccaat tagccacaga tccaaaaact
    23161 ggacataatc tgcatggtgg tgctcctgga tttgaattaa aaaaatggtc ttatgtcatt
    23221 ttgaatggcg aaaatgaagc ttctgtcatt tttactacta caagtcccga cggagaacat
    23281 ggttttccag gtacaatgga tgtggaaatc cgctatactt tgacaaaaga taatatctgg
    23341 cgagtgacct ctcgcggcac cagtgaccaa gatactttat tcaatccgac aaatcatgtt
    23401 tacttcaact tgacgggaga tgcttctcag tcaatcgatc aacatgagct ttggctcaat
    23461 agcgaagctt atgctccttt gcgaacagat agtattccta taggggtaaa agaaaatgct
    23521 gcaggaagtg ctttcgattt tcaaatacct aaaaaattag cgtccgtttt tgctagtgat
    23581 ttggatcaaa aaaatcttgt tgacggaatc gatcatccat ttttcttaaa agaaaccggc
    23641 ttgggtaaag aagccgctcg cttgactagc cccgataaaa gaatccaagt aggtattgct
    23701 acagatgctt ctagtgtggt tattttcact gcaaatttcg gcacagaaac accagaaatg
    23761 cgcaaccgaa aactggcaca tcacggtggc atcacatttg agactcaaac agctccaggt
    23821 gcagagcgct tctcagcatt tggttcgatt catttaaaag ctggatcagt atttgaaaca
    23881 gtgacagaat tcaaaatcaa aacaagaaag gaataactca tcatggaaat caaaagaagc
    23941 ttaaacacaa aatttgccga aacattcgga cctaaaaaaa ctacacaata cttctcacca
    24001 ggacgcatca atttgattgg tgaacacaca gattacaatg gaggccatgt cttccccgct
    24061 tccatcactt atggaacgca aggtgtagca gcacctcgag acgataataa aatattagtt
    24121 tattccacaa actttgacga tatcggtgtc attagtttta ctttagatga gctagaatat
    24181 gataaaaaag caaactgggc aaattacgta aaaggaatgg tccttaaatt aaaagaagct
    24241 ggacactcaa tcgatcatgg gtttgagttg ttagtggaag gaacaatccc taatggtgca
    24301 ggactttcca gtagcgcttc attagaatta ttagtaggtg ttgtattaga agatctgttt
    24361 gatttaaaaa cagatcgctt ggaactagtc caaacaggta aaaaagtaga aaatgaattc
    24421 atcggtgtca attcaggtat catggatcag tttgccatcg gcttcggtga gctagatcat
    24481 gcaatcttat tagatacaaa tacgctgaaa tacgaaatgg ttccagtgaa actagatggt
    24541 tatgcaatcg tcatcatgaa tacgaataag cggcgggaat tggcagattc aaaatacaat
    24601 gaacgccgta gcgaatgcga agaagccttg gcacgtttgc aaaaaacttt gcctatctct
    24661 tctttaggag acttagatga agaagaattt gaatcaacta ttgaccaaat cggcgatgat
    24721 acattgatac gcagagctcg ccatgccgtt tctgaaaatc agcggacttt gaaggcaaaa
    24781 gctgaattag aagctgggaa cttagaagca tttggtcaac tgttaaatga ttctcaccac
    24841 tctcttcgct atgattatga agtaactggg atcgaattag atacactagt agatgctgct
    24901 caaaaacaag aaggtgtctt aggtgcacga atgacaggag ctggatttgg tggctgtgcg
    24961 attgctcttg tcaaagaaga aaatatacct gccttcaaaa acaatgtcta tgatgaatac
    25021 atgaaagttg tcggctatgc tccggaattt tatgttgcac acattggaaa tgggacaaca
    25081 aagatcgatc cagaagctgt tcatgtttga taaaaaaatc aacgatctca gctaaaaaca
    25141 aatagataaa aataacctct cagcaatttc caaatgtgag ccttttgctc gtatttgaaa
    25201 attgctgttt tttattgaca cggaaagaga acgtgtgttc taattgtgtt gaagaggtga
    25261 ttgttatgtg ggcgaatcaa aatccggtat ttgtttatga aaaagaacct tcaagagata
    25321 ttttatgtat tgactgcaaa tctttttacg cctccgtaga gtgcgttgaa aaggatcttg
    25381 atccccttac aactaaatta gtagtcatgt cctatccgtc cgattcgaca gaaacacggg
    25441 gtagcggatt aatcttggct tccagtcctg cagcaaaaaa agcttacggc atcaccaata
    25501 tcagccgtgc cagagatctt cccttcccct atccaagtga tttatatatt gttgccccaa
    25561 ggatggccta ttatatggaa aagaacagag aaatcaattc tatctataaa aaatatgcag
    25621 acgaagcgaa ccatcatgtc tattccgtcg atgaaagctt ccttgatgta acaaatagca
    25681 tggcactctt tggtaaaaca aatgcgtacg acatggctaa actgatccat aaagatgtgt
    25741 ttgagcagac cggaatctac acgactatag gaattggcga taacccgcta cttgctaaac
    25801 tagccttaga caatggctca aaagacggaa agcaaatgtt tgctgaatgg cgttatgaag
    25861 atgtaccaga aaagttatgg aaaatctcag atatcactga cttttgggga atcggcaaac
    25921 gaacagcaaa acgtctgaaa caaatgggta tacattctgt ttatgattta gctcacgcgg
    25981 attattatca cttgaaaagc aagctagggg tcatcggtac tcagctttat gcccatgctt
    26041 ggggcatcga tcgaagtttt ttgggacaag aatatacacc aaaatcaaaa agtatcggaa
    26101 atagtcaaat cctagataga gattatacaa gacgagaaga aatcgagatc gttatcaaag
    26161 aaatggccga ccaagtcggc actcgcttga gacgatctgc agcaaaaaca caagtagtca
    26221 gcttatgggt aggatactct ttaggctatc ttgatccaaa tggcaagacc gggtttcacc
    26281 agcaaatgaa aatcgaagcg acaaatgcta gcagagatct agcagatgct ttattgacca
    26341 ttttccacca gcattaccac taccaggata ttcggcgtgt aggcgtgaac tgttccgacc
    26401 tcgtatacgc gacaggttta cagcttaacc tttttgaaga tcctgaaaag cagataaatg
    26461 aagcgaaagt tgattttgtt gtcgatacga ttagaaaaaa atacggcttt caatcaatca
    26521 tccatgcgca tagtctcttg caaggaggac gcgccatcgc acgtagttct ctcgttggcg
    26581 ggcatgcagg tggtatgagt ggattagaag gtggaaaaga tgagacggac agcaaaaaaa
    26641 ttcattgact ataacgagta tcgagatcgg ccatttggat taaaatgggg gactgctttt
    26701 gcgatggaag aactagtcaa cggtatcaaa aaaaatgaag aagaagcacg caaacagcct
    26761 cttcctaaac aacaaatgac attgcaagaa atcgatagta tactgacaga agccttttta
    26821 tctcgtaaag cagtaatcat tcagctcaat ttaaaagatg aatttggaag actcttggat
    26881 gaggtcgaag ggcacttcat tggtgaagcc tatgaagatt actttatgat tgaccatcag
    26941 ccgatattgt gggaagacgt acggtatatt gagttaaagg aaacaaaaaa atggtttgtg
    27001 acatgagagg ttgtgacaga agtgatcaac ttcaaaaaat aagaaaggat ttttcgaaaa
    27061 ttgcttctta aatttttgtg gaattttggc ttatttttcg aagaagcaac ttctggaaca
    27121 acgtatatta gatatcagag gttgtgaaaa agtcgcttag ttttgagtaa caagccaagg
    27181 cgctgatttc acaacctctg tctatctatc catctattcg gtaagattca tgcatgacgt
    27241 cttctttatt tagtatgtag agcccttttt cttctgacca ttcaacataa atcagctgtt
    27301 ttacatctgc atagccttct ttcttcaagt tctccataag ccatgcttca gtcaattgat
    27361 tggactgaag cactttatgc tgaatttgcc cttcatctac aagcaggatc gataaagctt
    27421 tgtcttcatc tgattttttc aatacactca tttgaccagc attctctagg ataacatgtt
    27481 tggcattttc aagagaaaag cattcttgct gtcttaagat tgctcgcaac tgctccatct
    27541 cgatatggtt atttgtcaac tctgtcatgt tgatgacgcc gtctttgatt agaacagatg
    27601 gctcgccttt gacccaacga ttgacctttt catttttttg gacgatacgt tcgataccat
    27661 aaatcattac tgcccaaagc gcaatggcaa ataggacatg ccctacatgg acattgtcat
    27721 cataggttga ttcttctaag atcccaccta aaacaagcgt atagactaaa tcaaacggtg
    27781 tgatttctga catggatttc ttcccaagca aacgtaccac taataataaa ccgatcaacc
    27841 cgaacaccag tttcattgta attgaaaaaa tcattttttc tccccctaac tatctgttaa
    27901 ttaaattata tatgctttct cacgaacacg catggaatat acaaggaaga aatgtatcct
    27961 tttactaaaa aaaggactgt gacactgtag tgacccccaa aagttagact tttttggagt
    28021 ggagaaatcc actccttttt tttatgctct aaaatgatat ttttacacaa ccgttacggt
    28081 tgttttacga aattggacg
//
LOCUS       DACDYJ010000034        27991 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDYJ010000034 DACDYJ010000000
VERSION     DACDYJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.034"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(52..279)
                     /locus_tag="IWM51_001513"
     CDS             complement(52..279)
                     /locus_tag="IWM51_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970939.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            complement(446..2416)
                     /locus_tag="IWM51_001514"
     CDS             complement(446..2416)
                     /locus_tag="IWM51_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970940.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(2431..3297)
                     /locus_tag="IWM51_001515"
     CDS             complement(2431..3297)
                     /locus_tag="IWM51_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970941.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(3287..3520)
                     /locus_tag="IWM51_001516"
     CDS             complement(3287..3520)
                     /locus_tag="IWM51_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9970942.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(3532..5688)
                     /locus_tag="IWM51_001517"
     CDS             complement(3532..5688)
                     /locus_tag="IWM51_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970943.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(5708..5929)
                     /locus_tag="IWM51_001518"
     CDS             complement(5708..5929)
                     /locus_tag="IWM51_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970944.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            complement(6039..6377)
                     /locus_tag="IWM51_001519"
     CDS             complement(6039..6377)
                     /locus_tag="IWM51_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970945.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            complement(6777..10418)
                     /locus_tag="IWM51_001520"
     CDS             complement(6777..10418)
                     /locus_tag="IWM51_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970946.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            complement(10421..12370)
                     /locus_tag="IWM51_001521"
     CDS             complement(10421..12370)
                     /locus_tag="IWM51_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9970947.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(12412..13470)
                     /locus_tag="IWM51_001522"
     CDS             complement(12412..13470)
                     /locus_tag="IWM51_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP9970948.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(13589..14044)
                     /locus_tag="IWM51_001523"
     CDS             complement(13589..14044)
                     /locus_tag="IWM51_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970949.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(14628..15431)
                     /locus_tag="IWM51_001524"
     CDS             complement(14628..15431)
                     /locus_tag="IWM51_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9970950.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            16412..17635
                     /locus_tag="IWM51_001525"
     CDS             16412..17635
                     /locus_tag="IWM51_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9970951.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            17670..18437
                     /locus_tag="IWM51_001526"
     CDS             17670..18437
                     /locus_tag="IWM51_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9970952.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFKDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            18627..19025
                     /locus_tag="IWM51_001527"
     CDS             18627..19025
                     /locus_tag="IWM51_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP9970953.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(19331..19726)
                     /locus_tag="IWM51_001528"
     CDS             complement(19331..19726)
                     /locus_tag="IWM51_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970954.1"
                     /translation="MKQEKRKMKQTQTEPINDTLADQLAGYYKLFEMSKAQRLIHYLW
                     KYKILFLFWEATLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
     gene            complement(19727..20104)
                     /locus_tag="IWM51_001529"
     CDS             complement(19727..20104)
                     /locus_tag="IWM51_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970955.1"
                     /translation="MKSKKETEPMKSTHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            complement(20094..20489)
                     /locus_tag="IWM51_001530"
     CDS             complement(20094..20489)
                     /locus_tag="IWM51_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP9970956.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEDLLEK"
     gene            complement(20556..20741)
                     /locus_tag="IWM51_001531"
     CDS             complement(20556..20741)
                     /locus_tag="IWM51_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP9970957.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(20823..21077)
                     /locus_tag="IWM51_001532"
     CDS             complement(20823..21077)
                     /locus_tag="IWM51_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970958.1"
                     /translation="MNYTDEEQITLEYVFTNEKGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(21351..21635)
                     /locus_tag="IWM51_001533"
     CDS             complement(21351..21635)
                     /locus_tag="IWM51_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970959.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(22129..22668)
                     /locus_tag="IWM51_001534"
     CDS             complement(22129..22668)
                     /locus_tag="IWM51_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970960.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKQQLDRIKEENNKPNLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            complement(22781..23047)
                     /locus_tag="IWM51_001535"
     CDS             complement(22781..23047)
                     /locus_tag="IWM51_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9970961.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(23049..23270)
                     /locus_tag="IWM51_001536"
     CDS             complement(23049..23270)
                     /locus_tag="IWM51_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAP9970962.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            23496..24314
                     /locus_tag="IWM51_001537"
     CDS             23496..24314
                     /locus_tag="IWM51_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9970963.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAVNENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWMVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            24318..24614
                     /locus_tag="IWM51_001538"
     CDS             24318..24614
                     /locus_tag="IWM51_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970964.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(24796..25149)
                     /locus_tag="IWM51_001539"
     CDS             complement(24796..25149)
                     /locus_tag="IWM51_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970965.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(25169..26224)
                     /locus_tag="IWM51_001540"
     CDS             complement(25169..26224)
                     /locus_tag="IWM51_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAP9970966.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            26440..26838
                     /locus_tag="IWM51_001541"
     CDS             26440..26838
                     /locus_tag="IWM51_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970967.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
BASE COUNT         8658 a         5037 c         4264 g        10032 t
ORIGIN      
        1 gactgagatc acctttcaat ctaaatacac ggttcttttc tggtaactta ttcatgcttc
       61 aaccacctca tactgttctc tcataccttt gacttcatta aaaaaaactc tcgctttttc
      121 ttcatcagta aagtttttat acaactgatt acaataatga ggtttttgaa gtttctgaat
      181 acctatacta acttcaaatc cttcttcatg ttgaattagg attacttctg tgtctctacc
      241 tagaaatatt cgatctaaaa tttttatgtt tggtttcatt atttaacctc cactataatt
      301 atttcactca ataaggtttc actttatgaa atttctatag ttctcttttc acaaaacgta
      361 ccatgttctc taagactgtt tttagtctca aatatacatg ttttttctta tcctttcctt
      421 atacactcaa aatcaaagat aaaacttaaa gaataaactc tccaataaca aaattattag
      481 tcagatccac tggtctcctg atctgaaatt tattacttct tcctgttgtc aaatactgtc
      541 tcaaatcaac agaaagtttt tgatcatctt ctccttttct agcaggagca actttttttt
      601 catacttcat taactctcca ctggtcatct ttccaatgat agtgtaatta aaatcacttt
      661 caaaactttg gtaaattttt ccatctgtat tgaatatatc agctgtttca atatcttctt
      721 tgtattcatt gtcttctcct gaaccgattg tatcaaatag aaaggcaaga cgaattccat
      781 cttcttcaag cattgttaat ctttttttaa ttagcccaac ggtttcaata gataacttat
      841 cgaagccgtg aatcataaca atatcatctt tttttgcttg tgatgtgatg aaatcaaagg
      901 tatttaaaaa ttgagcttcc ataacattgg tttctgctct tagatcacct aagttatagt
      961 aaaactgtaa tttgtcactt ttaccgattt ctttcagtgt cgttctttga ttaaacatcc
     1021 tagagaagct gtctaatgct gttctcaatg tgtcttttaa tacaataata cgatcgactt
     1081 ctttttcagt accagatttt ttggcttttg tttcagcatt agtcaaatag ctaataaaat
     1141 ccccaagaac tggcattgta tcactactgt tcaccaaaag ttttgaacgt tcttcataat
     1201 taatatccca aagtttctga cttatataaa agttacctaa ttcttttttc aataaaactc
     1261 tagttgcaga atctaatgtt ctgcctgcta acaaataaaa aatctgagtt agtttatcga
     1321 gattccgtct atataaatca ccaatgtcct tttgattacc aaaaagctcc aaagagttaa
     1381 gaccttcttc agccaaatta accttttcta ttacactgtt taacgaaggt ttggcaacaa
     1441 atgttctacc agttggatca ccaaagtaat caaatttatt taagacaata tgaaaagttc
     1501 gtttatgatt tgccattgca ttattagcaa tcaattgccc ccaaagtgat gatgctgaga
     1561 ttttttcatc atagccttta attgtagcgt ttttgtgtga tgccactaaa actttacttg
     1621 taaaagaatc atccagtgcc ataaaagcta caccacttgc gtagctagta gacatagtgc
     1681 cgatagccca tccattcgca tcatctaaac ctttgcgaat tgcatgatca tttcctgcaa
     1741 aatccgaact catagacgta tactcataga cattcccgat tggttcactt aaaagtctat
     1801 caaacagatc acgttgatct cctccatatg acataagctt taatccatac attttttcat
     1861 catacaaccg ttggagttta attagctgtt cgctgatttt attaggagaa tcggatacta
     1921 ataggatttc tagcttccaa tcaataattt tatttgccat gctgtctagt aaagatgctg
     1981 tattcaaatc cataatccgc ataacagttt tgctaatttc agcatctgaa ttttttcctt
     2041 ctaaattatt ttcataacta ttcgttgatc ccccaacatt ctcgtgcatt atttcttgtt
     2101 gatctttttg tgacactggc ttatctgctt caattagata ggcttttacc ccttctactc
     2161 tcacttcagg aatagcttta accatccaac caaatccata atttctattc attccataag
     2221 tattcactaa tttcacaatt gttccccacc gcttacctgt cctaatgtaa gatggtgtaa
     2281 attgataacc aactaaagat gttaaatcac taacaaaggt attattcact acctttgaat
     2341 ttctagtttt ttgttctttt ttagtttttc tctttttttt gaaatttaat agttgtgatc
     2401 tacccataat ttccatttaa tctctcacct ctatttcagt gcataaatat cagataatat
     2461 ttcttttgtt tgtttagctg acaactgagt gacactataa taaagtcctc tactcgtata
     2521 gttttctaaa cgttccacac cttcatccaa taccatctta gattgagatt tcaagataag
     2581 atattggatt actgttgttt tcacaccatc aatatattct gatacaaatc gttcttgctg
     2641 atctattatg tcaaagactg ctttgttact ccttgcattt cgtttttgta ttttcatatt
     2701 tcgagtttgt ctttccgtat tctgaggttg agatgaagtg attttgattt cagctaagga
     2761 acgatctctg gtattttgat attgtattga tttcgattcc tgagccataa cttcttctgg
     2821 aaaagcggtt agtgatgtag ccccatctag attgaacatt aatgctacac tcccatctcg
     2881 atatacgatt gttccatctt gtttcacttt taaaatctct gtgcctcgaa atggtttctt
     2941 tttccccata gatactcttg ctgaattaac tactgatttg aacagtctaa tagaaccaga
     3001 aatcaatgaa ccactagatg gatcaataaa catatactgg ctaattagaa taagaaatcc
     3061 aattatcatt aataacgttg gaacaaaaat aaatttcagt ttaatcgtat cagaaactaa
     3121 gtaaacaatt gatccaagaa caacgactgc aacaactata gctgatacaa ttaatctttt
     3181 tgctgtaaca gaataggtgc tattgacatg cttttcaagc cattcaactc tgacaattgt
     3241 ttcagaaggt ttttgatgta agttatgttt aaatccatag tttgagttat ctgccatcag
     3301 aaatacttcc tttcacgttc aacttacggt aaacacctac agttatcact attgcaacaa
     3361 taagcaagcc aattaaagat attgacttgc tcattgtctc gttcgtttgt ggtagttcat
     3421 ttacctgata cacgagctgt gcttcctgta agtgctgaga agaggtcatg ttttcttgat
     3481 tgttttgaat tactggataa actatttttt cttctcgcat aatagccctc cttagttatt
     3541 atcagataag tgagcttgaa ttttgtactg atttccgtct ggagttttca caactgcttt
     3601 ttctccatca atagaaatca cttcagcctt agttgttttc ccatcaatac tgatgacaac
     3661 atctttccca actttgaact ctgtagttgg tgttgcttca tagctactat ttgttgaagc
     3721 agagttcgta tttgtcccaa tcgaattttc cactctcgaa atagatgttt gagtttgagc
     3781 ctgtgcttgt tgattattat gactaataaa gtaggacgat cccaagatcg ttccgcaagc
     3841 cattgcagcg acagaaatga tagtagccat ctttttactt gtaaaatcac gttttgaact
     3901 tgattgattt tctgtagcaa ctgctaatga tttcgttttt tctgccattt gagctagatc
     3961 acttcgggaa cgagataagt cttctcttaa agcttcactt tgttctgcca acttttcgct
     4021 atttaattta taaccctctg ctaaatcttt gacctctttc attcgatctt tatattcttc
     4081 gatagttttt tgttgctcta caagttttgt ttctaattct ttcgtgttgt tagctacctg
     4141 cttttcaact tcttctttgt tcgcaaaata tttgacaacc acatctgttg ttttctcttt
     4201 tgtatcatgg aactcagctt cattttctga ctttgttttt tcgacaatct ctaaagtttt
     4261 ttcttgcacg tctgttaatg atttttcaat ttctggtttc aatgaattga taattcgttc
     4321 caccacaact tcttttgctt cttggctttg tttcaatgca tcgtttttaa tagattcaat
     4381 tgcttcttgg tactgttgat caagactttc ttcagttgct tttctatatt catcttcttg
     4441 ttgtttaact tctggtaaat gctcagcatc ataccgttgc tctagtttct ttagttcagc
     4501 atccaaataa gcctgtttcg ccatatcata atctttacga atgatattgg atttttccat
     4561 cactaaatca tcaattttag atgcttctgc ttcgtagctc tctttagcaa ccgatagtgt
     4621 atttgtaaac tgattttgac catttaatga ggttgcttcc tctatccgca aaagctcatc
     4681 agctaacatt tgctttcctt tgaggatcaa ttgctccttc gtcttattta attcactgtt
     4741 gattttatta ttagcaatag agatgcgagc gttcatactc tctttagcta aaaacagcaa
     4801 tggtttcaac tcaggtatgg atagcatctc ctcgtttaaa tcgacagggt cattaatctc
     4861 atctaaaact acaggttgaa atttgtcagc aatgatctgt tcaaaatttt tttctaaaaa
     4921 atcagcatct aagtttttcg gtacatcact ttcagatgtg attttttcag cttcataaac
     4981 ataattttct ttgtcacttt gggattcggg tttttcatca atttttggtt gttggtaggt
     5041 ttctgtttgt atttgtgttt cttctatgct tacttcttgg ttttctgctt cggttggttt
     5101 gttatcatcc acaaaaagag cttctttttc atctggtaca ttttctgcta cggcttcaaa
     5161 ttcaacttct tcagaagagt tgaaggtctc agcaattcgt tctaatccat tagtagctac
     5221 ttctaattgc tctactgcgc tatagaagtc tgtagcttct tcaacttctg attcaaacac
     5281 attaggatca tccggaataa ctaataattg accactacta tccataaaac ggacacctgc
     5341 taatagcttc tctttaaatt tttctggtag atcagctaca taattcaaac ttgacttcaa
     5401 tagaacgaca tacttctcag aatcgagaat agcatcatcc tcatctataa ctccctcact
     5461 gttgagcttt tgtacatctt caattaccat ataatttttc tttggtaaat ctttggtcag
     5521 caacgcatcg aattctttcc atacctttgc tttagctttt tttgccttcg cttctttttc
     5581 ttcttttttg tacacttcga gtaattgtgg cttcaaagag agtgcaacta caggaactgt
     5641 ttctgccaca taattaatag attcggtaat atctttgaat tttgccatta ttgtaaaact
     5701 ccttttttca gtcaatagtt cttttctgtt tatctagcat atatacatat gctttttcga
     5761 atttaataag cgtttcaagt ctggcttttt caatcttttt actcccattt cgataatcat
     5821 agaactggtt aacattcata ttcattatct ttgccagttc ggtacctttc agactgctca
     5881 ttaatacttc gttggcgtga gctaacattt tttctctatc aaaaaccatc ttacatttac
     5941 tttcttccct cacttctaaa ctacgcttgg agcgtagttt tttggtaaaa agtaaattga
     6001 acaaatcggc atattatcca aatttgttca attcactttt aacctaacag ttcatttttt
     6061 ggaaattttg aaactagata gataccaaca actggaataa gtgctgatat gatattagct
     6121 ttttggttca aatcattacc tgttttagta cccattgtgt aaagcataac ggttgtaatt
     6181 acaggaataa taaagattgc aataatcgcc ccaacatatt ttaatccatc tccccatttt
     6241 gcatcaccaa aaaatttcat tgcagtgaat cctggtttca acgaaaaaac gaatgccaag
     6301 aaatacaaaa caacttgtac taaagttttc atatcaggcg tcattccgct aaccaaattt
     6361 ttaatagaat cgaacatata aaaacctcct ttttaatatt tttaataagt taattatatc
     6421 atcgacaaat cttatttgtt cacttgtcaa tgatctaact aagaaaaaga cgtctcgata
     6481 tttacatctt tatgttatca taataacata aattttcgat gattaaaaga gattttccat
     6541 taaattattt cacttttttt attggctaaa aatgctggtt tatcaagttt ttgtttagat
     6601 aataattctg ttctttacca gcataagccc tttttaatta tacaactttg aacagtaaat
     6661 atttaattca tttatagaca atttaaaata atttttttgc ataaatagaa attttccttg
     6721 acatcttcag ttagaaatca aagtaaaaag aaggagcaaa tcaatgctcc ttctttttac
     6781 tcgtcttcag acttattttc agttgatttt tgattaccgt attttacaat atagtccttg
     6841 actgacatgg tagatttctt cactacttta ccagttttgg atgctcctgt agcagtagcc
     6901 ttggcaacct tagccgttcc tttacctatt ttaatagctt cgtttttagc ttcataggtt
     6961 atccctgcta gactaccttt tggagctttt gctaaagctt tatctgctga ggtgtaaaca
     7021 tttttaccag tttttgatag atattcaacg gctgatttag ctgttgatgt tttcttggtt
     7081 gattgattag atttccctga attatctgaa gtagatttag aattatttgt tctattattt
     7141 ttatttgtca gttcaggttc tctaattgat tgattgtctt tcgacattcc tgatccaata
     7201 tttgtttcat atttatctgt ttgattaaca ttaggagtag gaatgaactt tttcttcaca
     7261 tctgattgtt ctttatccgt ttcttttgtt agcgattctc tgtctttaag tactgtgtca
     7321 ttccgaatag cactattatc aaagttgctg ttaagctctc ttgactgact ttcaccagat
     7381 gctccatctc ctaattctcg atgcactctg tctgacacgt cctcaccact catttcattc
     7441 ctcagttctt gatgagtttg atctaaagta atctcgtctt cttcattctc aggaattgca
     7501 acagcaattg gcgtatgccg ttgttgttcg agttctctat ttcgatctgc cacaagcgca
     7561 tctctcgtct caatgatatt cccatcctca tcgactaaat ctccattcac attatatcca
     7621 tcgctgtgtt taccaagttt attaccaaac tcatcaacaa gatttccatc ttcatcaact
     7681 aaacgacctt tttcgtcaac taaacgtccg ttactatcat gaccaaaatc accatttagc
     7741 aacctgcgtc cattttcgtc aacaagattt ccatcttcat cgactaagcg acctttttca
     7801 tcaattaaac gtccttcgct atccatacca atattaccat cttcactatc ttccaatggt
     7861 ttaccattag tatctataag gtttccatct tcatcgacca aacgcccttt ttcgtcaata
     7921 aagttgcctt tagcatcttt accgtattta ctatctttta atatttcacc attttcatcg
     7981 acaaagtttc cgttttcatc aaccatacgt cctaaatgat caatatattg ttgggagtta
     8041 ttacgtagtt gatcttgacg aactaaatct ttgaaagagg tattattgat tgtggaacca
     8101 actccgccac cgttgaatga cattccttct ggtgtttcag gaattacttt atcagcaatg
     8161 gacttagcat cagcattgct atctcccata ttgttcaaac gttcagccat tttttgcgct
     8221 ccaactgctc caagacctcc tgcaaccata tcttttactg agctgttgcc accatcataa
     8281 tagggatttt tagctccttg gcttttcttg attgctttag aaacgccgtc cacaactcgc
     8341 acagcatcat ttctattacc aaataataac ttagcacgtt catcaaaccc aaacgctact
     8401 tcaagtggta agccaataag catattaatc aaattattta ctttttcgcc ttttttaaag
     8461 actaaaagat atcctacacc taaaatcata gcagatcgaa caatatttgc tacgccatcg
     8521 ccaactaatg tattaggaat gaatcccaca gcagtatcac caaatgcacg aataaaagta
     8581 atcagaaaga taggtaattg tagtccgatc agagatgttg taacaacacc taatgaaatt
     8641 gccatcacac ctgctcctga tgcaagtgat cctgttagaa ctacttccca aatccgaata
     8701 aattcacgtt taaatgcttt cacgatattc acattgaaca tcgcccaagc acatgctaag
     8761 taaagaactg ctaaaacgat tgattcagcg ttcatcgcta gccacgagcc aaacttaccc
     8821 atagctccat gaccaactaa ggatgctgta cgccattctg tttttactgt tgacatattc
     8881 ttagcactgc cttgaattcc tgaaggagcg atattatacg cataaaactt agctcccgat
     8941 gtatcaagtg ccgattggag caataaagct accgactgtg tgctaaaagt tgcattgtta
     9001 gcaacgcccc aaggttgagt agcatcaaag ttatgcgctg ttccttttcc atcaggcttg
     9061 cgattgtcgt ctgtcacatt ttgatttgct gaccaaatat atccatttaa attggttggt
     9121 ttcttaattg cacttccttc ttttccatcc cctaaattat agcctgtcca aacagcaggc
     9181 gctcttccca tataagcatc attttcggcg acattaacat caccggcata agtattaata
     9241 ttgaaagtat cattcgatga ccattctttt aatagagcca taccgactga attagaatct
     9301 agatcattat tttgtctggc ataactgcta gtattgatcg ctcttatcat tgcagatttt
     9361 ttagatggtt cataatctgt atcaacgaac ttgatttcgg atgtaggctt tccgtcaggt
     9421 acatcaccac cagtatcaga tgatggagca agattttgag tagaagccca aagtcttgtg
     9481 ttaatgatgt agtcagaaac aacatttgtt ggtatatctc catctttttt tgctgactgg
     9541 atgccgttga tataaactcc caatagcgat cctgccactg gtaagaatat aaacaataca
     9601 aaaatacgaa caatccattt ttcaagtact ggtgataatc ccttcgagtt aacaccatct
     9661 ttcgacaata atttcattcc ttgataacct agtattgctg tgaaaaagag catagataag
     9721 cttgctatcg ctttaaataa ttcattattt aatcccaaat tgttaagcaa gctagttaat
     9781 gctttagata ccggattaga tacatccgat ccaccttctc cccaccccat aagagaaaat
     9841 atattcatac tcatgaaaaa ttttccaagg agttcattta tggattcagc aatattagaa
     9901 aggattaaac taattgatcc gataaaacta ccaatcgtcc taccaatact tcctagaatg
     9961 gatgatgctt gactggcaat agcatttcct gagactgttt caatatatcc gtagtaacta
    10021 taagttgcta aagtagatgc tagttgagta cctgcattac cccctgcaat cgaattacct
    10081 tgtttagaaa tgtcagaata agggaaatcg gcactgatac caccttgtcc taacaaattc
    10141 atttgtccag ttttagcggc actatcatta acttctttat ctcccattag gatacgagca
    10201 tatagtgtcc acattccttg ttctgccggt ttactttccg catatgcaac gttcttattt
    10261 ataactgttt cttgtttgct atcttgattg tcaggagttg gatcattcat tccaaaagga
    10321 gcgcctaaag taactgctaa tactcctcca ataactagac ctcttacgac ccatcgtttt
    10381 attctaaggt tttgtagtaa atatttcttg aatttcatag ctacgcccct ccaatcgaaa
    10441 atacaatctt gttttcttta ctttcagcaa cggaaggctt tgcaataatt tcagcattag
    10501 aatcaccttt tccttcaaac gctccggctt cactcaactt aacatcgaat atttttgcgt
    10561 ttggataagt aattattgct tcagtaatcg cttcttcaaa ctcagattca actaaatgca
    10621 agatattcct tgcaccccct gatttagaat cctcttgtga agccatgtca aaaactaagt
    10681 gcatggagag atcgtctgca taaaaatttt gaccatatac tctttctttc ctaggtggaa
    10741 gacgtaaatc tatatttaaa tgttttcgta ctaattcagc actcttcaac atttcattat
    10801 aagcaatttc cagaagagtt tgttcggata aagcccaaaa tggaactatt cgtccaaatc
    10861 ggttaataac ttcaggcttt agaccttcac caatcaaggc ttgtttgatc cctgatgcga
    10921 cttttcggta ttctcttttt agttcagtgc tatattcatt agaattttcc ttgtttgtaa
    10981 cacgagatag tgagctatat ctgtttaacg aatcgaacat ctccgcacct gcatttgttg
    11041 tcgcaaataa aattgtattt gacaatgaaa cagtgtcgtg aacttctcga tcatcccatc
    11101 ctctttcagt aaaacttatc ctaccttcat ctaaaattga taaaaaggca tcgagtgttt
    11161 ctacaccagc tttctcatat tcatcacaca caataatcgc agaaggatca tgccttacat
    11221 gagtacctat gagtcgtcta aaacgcattg catcatcttt tgttttgtag tccggcatat
    11281 taactaagag aactttattt tcatcgccat aaagtcctct tgccacggct ttagccattg
    11341 cagttttacc gacccctgtt gatcctgtaa acagagcagt tgcttttggt tgctttcgac
    11401 ctttaaaacg atcaaatcca agcttcatac gtttaaacaa cattgttata gtataaactg
    11461 ctaacggttg cccttttatg gactttaatt cctcttgaag tttttccata tcaaaatcag
    11521 aatctagctg aatattatac ttaccaaata tttgcaatag actttctcga ttataagcat
    11581 ttccgtctac tcgatctttt gcaatcaaat cctccattac ggcaattgat ttagccggtt
    11641 cggacaaatc ctcacgataa agcttgtttg cttgtacgat ttcttgaagt aattcatcag
    11701 gaatcctttg cgataattta aaagaagcct gaggatcatc tttaacagag gatgtattta
    11761 gccattgtct caaaatatcg acgacaacat cgctttttag ttcattcaat tctaacggtt
    11821 gtaaccgtct ccctaaggca tcatctcttt tgatatagtt ttcatattct tttcttgtag
    11881 tagcggtaat caattgaagt gattcatttg ctaacggttc ttttagcgaa tcacccccaa
    11941 ttttattgcc actaccaaaa gagctaacaa ctttatgaaa ctcgtcaatg aagaggacaa
    12001 attcattttc tttgtcctca gattttaaaa ttcgctcata ttctttcaat cttggaatta
    12061 atgtttctaa acgtgtttgg aggacatcat ctcccccaac acgcaattgt ccgattgata
    12121 atttgtatac ttgtactttc tttccatctt gtcgctgtct ttcagcccat tcacttgcaa
    12181 gtgctgtttt acctgttccg gcttcaccta acaagatagc ttgcggtgtt tccggtctat
    12241 tcaagatcag tgacaactca tggagttcgt catcacgtcc cactaactta tgtttcggtg
    12301 gtttcattgg tgtgagagca tgattaaatt cagtttgaat attcttaaaa aactcggtac
    12361 tattttccat ctgtttgtgt tcacttcctc taaaataaga ataatttact tctagtcagt
    12421 cagagttagg tacttaaatg tcgtcttcat gtctccaaaa gaatctttgt taaagtcagc
    12481 atttttgtat tgatcaatca atttttgtgt atcattgatt gctttaattt cggtctccat
    12541 tcgactgtct agttgacgaa tactttcttg cgaatcttta tcgtcaggat tttgttgtaa
    12601 acgttccaga tactcagctt ttgtagcttc aattttcttg atgttagtat ttgaagcatt
    12661 taactgatct tctaacttac ttacaacatt attcttactt gtaactgcta caacttttga
    12721 ataatcaata tcgccatctt tggtaagaaa gcttccttga tagacattag ttgaatctga
    12781 atatggattt attctgaaat tgatattatc aggagtatta acaggttttg cttttttccc
    12841 aaaagaaatt actccttcat tattaacatc attcatagaa tattgagaaa gtgcttttgt
    12901 aactgaatct gcttcattca cattgacttt ggtatcatcg tcagctgtat catcaccaga
    12961 aattagtgat acttggtttg caataaaaac ttgataagct ctttcatcta atttaccttt
    13021 taggaataag aaagcatccc catcagtact taacatccca tagctcattt caagcttttt
    13081 aggacgatct ttttctttcg ttgccatata taagttgtag ttttttcctt gcgtagataa
    13141 caactttcta gcgttttgcg agtaaccgag ttttactact gttacatctc tgttttgatc
    13201 tgtccacaca tctgaaatag tgatctctgc tcctgatcga gcaaactcca atgaagagcc
    13261 tataggtgtt gtattgttca cgagataata attatagttt cctaattgat aaatgtagct
    13321 acccaagcct gttaataaaa aaagcactcc tagaaaagtt gcaagcataa tgcgtaatcc
    13381 gtgacgtgtt ctcttatcct tatcaccaag caaccctcca ataatactgt attgatcttt
    13441 ggtagatgtt gaattgacgt ttttttccat tagtttgtct gccccctttc ataaaagagc
    13501 aaacaatcag acgttttttc ggtaattcta ggttcgggat tttgaatcat ttcggtatat
    13561 tcttccccat taatatttaa cacaaaaatt atcttccttt cttcgttcct ttcttttgct
    13621 ctttattttc caaataatca atgatttttg ctattgtacc aagatagtaa ttcccctcgg
    13681 ctttgttttt tcggattttg ctaaccattg gagtaggtac aatctcttca tctctcaatt
    13741 cactgaaaga ttttttacct agatattttc caataacaga ttttaaaaaa cgttcaaaat
    13801 ctttctcgta agttgttttc ttgcctttta gttcatggtt catattgtca acgacttttg
    13861 cggtttcaat aattgtttta gttctcagct taataatttc atctggaatt ttcaatcctc
    13921 gtaatttcat cgttgtacta actggtaacc catgctttac tgcatcagca atactaaaac
    13981 tttttagatc atctagtaag ctctccatat agttaacaaa ttcatgccaa ttaatagcat
    14041 tcattgtctc accccttcat ttttttaaaa tgaggttcgg gcgtaacaca tttatgttat
    14101 tataatatca taataacata aataaatcaa tcagcaaatt tagatatccc ttaaagtatc
    14161 agcaattatc caacgagata tttattttag ctatttcagt catcatgaaa gactaattag
    14221 caataaaata actaatatta tccatttatc agacgaaaag aaaaaactta tctaattact
    14281 ctatattaac ttttaacaaa tagttgactt gtccaaaaat gttaccttca atctcacaaa
    14341 gatattaaga tgtttcctac caaaactgat atcaccggac tttttattct gtgttgtcca
    14401 aaagtgttac ctttagtcga tcaacaaatt aagaaaaatt gtttttttct tgacttgtcc
    14461 aaaagtgtta cccatagttg ttaaaatttg tatagattag ccattctttc aaatattatt
    14521 tagactgaaa cttgcttaca aaaaaacttc ttgcatttat tgtccaaaag tgttacccat
    14581 acttttttaa agaagcaggt tgtattaaaa ttacaacctg cttcttctca atcttcttct
    14641 aaccaattat gcattgaaat ggttggcaag tttgattctg gctcactaaa agtgaatctg
    14701 agccgatcaa ctcttcttcc tttctttgca tagatttttt cataatcaaa agtttcaaat
    14761 acctgtattc cgttttcatc aggagctaaa aattcttctt tcgcctgctc tagtattctt
    14821 ttatcaatat ttgtcattct ataacttgaa ggtatactta atgctcttct aaaatcttca
    14881 actttgacta cccaataacc atttcgccac tctttatttc tgtattgcat gagatgacga
    14941 tacaattctt ttgtgtagct acttcttagt tttgtaaaat tatcaagttc aaaacgagta
    15001 aagtttttgc caattgagtt taagataaat tcaaattcct catttgcctt aattgtaact
    15061 gtttccttct gtttatctat ccggaatttt tgaaaaagcc acagctgttc atagacgtct
    15121 gaatcttcaa aacggtaggt taaattacct agtttgtcac tcaaatcttt taacacagaa
    15181 taaaaactgt ccttttctct tctgtcgtat ttgaccaaat aacgaaagag gttgaaatca
    15241 aaagttactt ctgctgttcc ttgacgtttc atttttgata aaatagccca aaacacatcc
    15301 atttcaacag gcttaaatcc tcgcaaagct gttgtattca gaccattgtt ataacgcaca
    15361 attgcatttg tttgttctga tactaaatct acatcactat caataatctt ttcggtaact
    15421 tcttgagaca ttgattactc tccaatcttc tttttgcgct cctttaatca tgttaatctg
    15481 gtactaatta aaggaaacaa ttttttctgc ttgttaccta aattaaatca caactaaagg
    15541 taacagtcaa caaagatttg ttactaatct aattttctat tttttcgcct tccaaatcaa
    15601 gaaaaccgaa ggtaacgtct tatcctagtg tgatacctca aatgtccact tttgttacct
    15661 ttgctgtcca aaactgttac ccttactgtc cacttttgtt acctttactg tccaaaagtg
    15721 ttacctttat acccctttaa tacttactct cccaagagat taagcctccc ctaacagata
    15781 tattaacaga tatttaacag atatattaac agatatttaa taaaggagga ttactcctct
    15841 cttatttcaa aatcttaaaa ataaaaaatc ttaaaaaaaa ctaacactat ctaggagttt
    15901 attttgcttg tgtaactttg aataattgaa aaaaaggaaa aggaggaagc ttcagcttcc
    15961 ccttttattt atatttataa tatatatttt aatctttaaa actttagcaa cctccaaaaa
    16021 ggctttatat caatcttaaa tagattttag ggtggacata aagcgatgcg aacatggaca
    16081 aaaagcgatg tgaatgtgga caaaaagcga tgcaaacgtg gacaaaaagc gatgtggtat
    16141 tccgaatata tccaaaaaaa gaaaaagtat gtgaatttat ttctaaaaac tagaaaaata
    16201 tttgtttttt aattttggta atttttataa tttcgtattt tattgaaaag gatttttatt
    16261 ttgaaaatgt tctaaaatag cgatttctgc tgtattttca atgaaatttg attggaaaaa
    16321 ataaaatgtg ttttactgac attgagttat gttataaatt tacgatacat ttcttattgt
    16381 aacgtatcga aaacaggtct atacttattc aatggaatcg taccagttcc aagtttttaa
    16441 gctcgtacca agcttagaaa gaagaggcaa tcgtatgaaa ataacagaaa agaatgtaaa
    16501 cccgaaattt gaaactaccg aaagcaatct aaaaaagttt gatttaactg ttactttatc
    16561 gaatcaactt gtagatagca ttgttgaaat ggatgctatg ccacaaaaaa tttatcttta
    16621 cgctatagga catcttgacc ctaaaaatcc gccttttaat aataccgtca aagtatctaa
    16681 aaaggcttta tttgcgttga tgtcttctag ttcatcttct tcaaaacatt caagattaaa
    16741 acaagttatt gaaaaattgc atcagcaatc tgtcattaaa attcaaagaa ctaaacctaa
    16801 cggtaagact gaatacaaag tcatctcacc gctagaaatt actgaattca atgatttttc
    16861 tgatgcagta tctattaaat ttagtagttc aattatgcct ttattaacga acttagaaga
    16921 tcattttacg caatatacat ttgatcaagt agttaacttg acaagcaagt atgcgatcag
    16981 tttttttcaa ttattcacta aagattttaa tctgtatgaa gcaaatagag ataacttaac
    17041 atggagtaca aaatcacttg atagattaaa aaatcctgtt ttctccattg cagatttacg
    17101 aaagaaattt agtattgaga aaaaatataa agcttttcga gactttgaaa gacgtgtaat
    17161 taaagaaccg attgcccaac tgaataaata cactgatttt aaaattgact atgagcttat
    17221 caagaagggt catacttata caaacataaa gttcttgctt acacgtacca tgtcagttcc
    17281 ggtaccttat aagcaagaac aaagagaccc tgcttattta aaaggcgcag aagataaaac
    17341 agagaaaatg aatcaatcgt acttgaaagc tatgaatagc aaatacacta aattactaat
    17401 caaaaacttt ttgatcagtt ctgccgactt atgcgatctt aaacttatga gccgacttca
    17461 gaataaagtt tatccgcttt atgatcgttt atccaaagat agaggatttg aatcagttgt
    17521 caatcaccta tcttacgttg ctactcataa ggaggattac acaataaaaa atactgttaa
    17581 atatctcgaa aaatcaatta cgagttattt gccttatgtg aacctttatg attaacaact
    17641 gaaaaaatgc gtgaaggaag gaaaaacttt tgaaaaaaga tgaactgaca aagtacaatg
    17701 aggagttctt agcaacaatt gatatgaaaa tggatttaaa gtcgtatgaa ctttatcaat
    17761 tgctgttcag ttatatctca cccacaaagc taaagaaaac aggcgtagta gatttacctg
    17821 ttaaagcgat tattaattac atggatgttg atcaaaatct tgaaacggaa ctaaaaacca
    17881 tagttaacac tttgcaaaga caagcagttt atgaaatatc gaatctagac aagaaatata
    17941 agacgtatac tactgtatct gctattgatc ggattcattg ggatttagaa gagcatagtg
    18001 tatctttcag tttttcagaa gatgtaattc ccttaattgt ctcaatcaaa aagaagtatc
    18061 cgttctttgt ttttaaagac ttagcatctt ttcaaaaaaa atatagcatc gtgttataca
    18121 aatatttggc gatgtattat aaaaaatatc ttatcgccaa aaataaagga gattttgaga
    18181 aagctgaaat atataagaat cccctgattg aaatagttaa aattcgtgag attacgaaaa
    18241 cagaaaatga gtataaaaca atatcgaatt ttgatagacg tgttttaagg gaatcagttg
    18301 aagaaattaa tgaaaaatca atgctgaaga ttgattttgc taaagttaga aaaggcagaa
    18361 attacaattt tgctgtccag tttaatattt ctagtgagtt tgatgaaaaa ccttatagag
    18421 aaaaaaaatt aggttaatgc aaaatatatc ttactcgttt gaagatgctt cagacgtatg
    18481 ttaagatatg tagtgccttg ggagcaaggt attaatctcc tttagaggtt aattagcttg
    18541 tgatcgccta tcaatttttt ggtaggcgat tctttttttg aaaccaaaaa gcaaaaatta
    18601 ttttttaatt agagaggtgc aatgcgatga aatacttttt ttgtgcagta ttcgaatgga
    18661 aaggaaatga atatgcagtt aaattcccag attttccaga cttatatacg catggtgaaa
    18721 ctttagaaga tgcttttgag atggcgcaag aagtattgga aggatacttg gtgcttttag
    18781 agttgcaaga aaaagaaatt ccatcgccta cgcctgttca agagttaaat gcaagaatca
    18841 agcgatctga gaatgttaat ttatttgtcg taaaagctaa tacgaaagtt gctaaagctc
    18901 gaaacaagca aaattatgat cgaaaatcag tgactgtgga acactattta aatgttttag
    18961 cggctgaaga gaatattaat ctgtcgaaag ctttgaatga tttgttgaga gaaaagttga
    19021 tttgatgtct ctcagatgat catattaagc aaaaacagct tgttttagga cgtatggatt
    19081 taagaaagac aaattatcaa agaatatatt aaaagctctc agaagcgttc tgagagcttt
    19141 taaattgcta tgtattatac tgcatctgct caaattgatt ttcttttcat gcttaccatg
    19201 tatctagttt tccatcaagt aatttttaga tatgtattga ggaataaata gcaaatttgc
    19261 ttgatataag ctcgaattag gtgctttgta atgtgttagg ataaattata caagaaaatg
    19321 tcaaaagctc tcagaacgct tctgagagct ttttatcggt aggcttgttt aacgtgatct
    19381 tgaaagatac ccttttgatg tttctctcga tccctgcatc gctaattact aagtgttctt
    19441 caagtgtgat gtaggtatat aaattaagca tcaaaatggt gtagataagc aaaaaactcc
    19501 ctaaaactaa gtgttgatta taaaaatctg acttataaaa aagaactgag aatgttagtg
    19561 ttgcttccca aaaaagaaac aaaattttgt atttccaaag atagtggatc aagcgctgtg
    19621 ctttactcat ctcaaataat ttataataac ctgctaattg atcagcaagc gtatcgttaa
    19681 tgggttctgt ttgtgtttgt ttcatctttc tcttctcctg tttcaattaa tctaaaattt
    19741 cagattgagc aagcaactca ttgtggtagt cggtttgaaa ttcaaataac aatttagcct
    19801 tcttaacgag gtttaaagcg ttcgttagtg attcacttaa tgcttcaaca tctttaactg
    19861 attttgcatg tttcattttt tcagtgtgcg tatagcagaa ttcaggtatt gagttaggag
    19921 ttgcctgttc aataaacatc tcaattcgca agttattatc ctcaaaaata attagatcat
    19981 tggcaaagtg tttggtacta acgctaatgc cagttgtatc ttttaataga ttattgatta
    20041 acgttcgaga gaaatagttt aagtgtgtag atttcatagg ttcggtctcc tttttacttt
    20101 tcaagcaagt cttcttctag tgctttttga agtaattgag agaagtttat gtttttctcc
    20161 ttccctaaaa gaactaacca ttccggaaca ctaacatttt ttctaattgt ttttgtattt
    20221 aagcttcttc ggaaaagttt aatgtttagt tggatgatta ctactacttg attggctttc
    20281 aaagttaatt cttccactgc tgatggttta ggaaattctg catctggtac actatatagc
    20341 gctaatccta aagcatcctt agcgtttaaa tatgcttctt caagactatt tccaaaggta
    20401 gatatttccg gtaagtcagg gaaaacgatg ttataagctt ctccttcttt ctcgaagatt
    20461 gccggataaa aaatattttt tgtcttcatt tcctcgctcc ttttctagtg taagagagga
    20521 tagtatgcct aataccaaat aggaaggcaa ggctgttaaa gccctgcttg cttccagata
    20581 ttagcttctg ttcctagtgg tacatcacca ttgtggatcg gaacaacgac cgttctttta
    20641 gtcttggaat tataaaattt tgcatgactg ccagtttgtc cagtcttttt gaagccgtta
    20701 gatttgagaa tttttacaat ttctcttgct gtcttaggca tagctaccct ccctttacaa
    20761 aatctattat acacattgtt gtgtatggaa tcaatagaaa aagaggatgt tttattgcaa
    20821 aactaataaa atgatttatt ggttttttca gcaaattgaa tgttattagc gttgcaaata
    20881 ttcataattt cccaagtatc attagaagta taggtatcta agaattcatt gacagttacg
    20941 gttgattcgt atggttcata agtcaaagcg ttctccaagt cttttgatga aatgatcaag
    21001 actttttcaa tttcctcccc tttttcattt gtgaaaacgt attccaaagt gatttgttct
    21061 tcatcggtat aattcatatt gtgttctcct ttcttgtatt aagaaaaatg gatatttcca
    21121 attgcatccc actcggcacc attgtggtat aagacgtgac cgagcgttac gcctttgaaa
    21181 tgctcttcta aatacatcta aacgcattca aggctagtac ctgctttgag attgtagaag
    21241 tctgcatcca ataccttttc agaagttatc ggatcaatgc gagttggtac ctcaataaat
    21301 tcagtccaaa tttctgcaaa agtcttacct tccaaagtcc acactcccta tcaattaatt
    21361 ccaaaagcaa atagagaaat caagcgaccg tcaatagtca cttcaacaac tgaaaatgcc
    21421 gttgaaacag ttttaacatc cacactttcc aatttcccta tgaaggaatt tttgtgcttt
    21481 agaatagctt tctttaactg tctgttatac gcttgacgac tgttaaactt tttgaattct
    21541 cgatccttga tcagctgttt aacgacccct gcattaattg tatagctttc tgcaatagca
    21601 acgtgagtag gttgctgtaa tgtagctgtt agcatattgt cactccttcc taaaagttaa
    21661 ttagcttgtt tacaattaca ttatacacac tagtgtgtat aatgtaaaga gaggtttttt
    21721 gctgatggat cagctgtgct aaacaaaaga aaacgataga gtaacaaaaa aagtcagcaa
    21781 agttaaaaag gaggaaacgg ctagtccggt tgctttgctt gtgtaagttt cggctttctt
    21841 atagatcaaa taggaaatta tcagtgatat tccagtaaag aataatgtac tagctgtcac
    21901 taaaagaaat aggttaatca tacgatacac tccttctgta atattggtat cagatacgtt
    21961 taaatgcgtt tctgagctgt tttggatcaa tggatgtttt agccctaccc taaaatcaaa
    22021 gcccagaaaa gcatttaaaa acgttcgtac ctaatacgtt tatattcata tacatataca
    22081 taaacataaa cataaacgta tacacgttta tgtttatgta tttaagtttt aggctacttc
    22141 cttaacatct cctgctgata gtcgagtgaa ccgagtgagt gatccatctt gtagtctaaa
    22201 aataaagcta cgtttgagtt tatactgata aacagcgaca tacgatccat cccagattga
    22261 gtaagtttta cctgcaacta ataagtcatc aaggtttggt ttattgtttt cttctttaat
    22321 tctatctagt tgttgcttaa ccattttttc ttgatcaccg acaaagaatt tcaaccattt
    22381 tttcggaatt ttcttaaagt aagcaggact gtcatttaag aagtagattg gtttgtacat
    22441 ttcatctcga aagatgaagc tgtcaatatc gtaggttcct tctgcatctt tgaaaaggat
    22501 aataccatag gtcttcgtta ctttggtatc tacgatttcc aaaccatttt tggcgtaccc
    22561 tttcttttct tcgatcactt catttgctga tgaataagca ccataccatc ccataaaaaa
    22621 tcgctccttt tgaaagttaa ttagcttgtt tacacttata ttatacacac cagtgtgtat
    22681 aataacaaga gggtattgtt cttttttagg taaagtggga tcgtaaacag tgattttgtt
    22741 gggttaacaa aaaaacagac aagccttatc agcttgtctg ttacttataa acttcacttc
    22801 tgtgaccaat agacaatata agaattgtca cttcatgctc gctaatatca gctattatac
    22861 ggtaggcacc taccctatat cgccagtagc ctgatttatt agctgttaga cctttaccat
    22921 gttttcttgg atcatcagtg cctacaagat ttttctcgat ccagtttagg ataacctttg
    22981 cctgaaactt atccattttt tttagcgctt tttgagcttc acgttcatat cttacttggt
    23041 aagtcatctt ataagcctaa ctcttttagc atatcttcgt gggaaatact ttgatcgttt
    23101 tcctcatgct ctttcattag ttccttgtag gtggcaaagt ctactaacgt ttctgctgtt
    23161 tgcagggaat tactacgcat aaattcagaa atgttatctc cgttgaattc agcaactttt
    23221 tcaagaaaat ctttttcttc tttagataca cgaatactta ttactggcat agtttatcaa
    23281 ctccttttca attacattgt actacgattg tatgacaaat gcaatagtta tatatatgaa
    23341 tatatacata aacataaaca tatacatata tatgtttatg ttgtagaata taaaagcaaa
    23401 tataaatata aacacgttta tgtatataaa catatacata aacgtgttta tagtgtataa
    23461 tggagttaca aaaggttaat aagggaggtt taatcatgga aaagaagttg tcaatcatta
    23521 ctggaaacat tgaaaaaggt ggaacaggta aaacaacaac aatctacaat ctaggtggat
    23581 atgctgtcga aaatttaggt atgaaggtgc tgtatattga tgaagataag tcgcaaaacc
    23641 tatcaaaacg atttcctgat gctttcagta aagcgaaaag agagaataca atcaatcatt
    23701 tatatcttca tggatcagct gaacctttag ctgtaaatga gaacatggat attttagtag
    23761 caggtgaaga tttaaaacaa gttgagatgc aaatgcgtga tatgcctaac aacagaaccc
    23821 tactgtttag atggatggtt gttaactatg aacagcttaa agagaagtat gatctaattc
    23881 tgattgatac tcataatgat gcaggtctac tagctcaaaa tgcttgggct gtatctgatc
    23941 ttgtcgtagg catttctgat ccatctaaag atggttttga agctttgctg aagcttggta
    24001 aagatattga tgcactagca aaacagttga ttgatattaa gactggtgaa acactgatgg
    24061 aaactaaata cgctacgatc ggtagcagga tcaaacataa tactaattca agtcgtgagt
    24121 tcctagaact ggtaccaacg ttatctaatt atcttggata tgtacaagag aaggaattgc
    24181 ttaatgcaac tagcttagaa ggtaagaata ttgctgagtt cgctaaggat aagaatatct
    24241 atcgtaagaa taaggatttc tttgaaagca cgtttacgct atacaacaaa ataattgata
    24301 cggcacaaga ataaggagtg attgaagtga aagaaaatag atttaaaaaa ttcgctgaag
    24361 ctgaagtaga agaagttcct gaagtggttg aagcagttga agaacctaaa caaacaccta
    24421 aagaaacaac aagttctttg gtagctaaac taaaaagtga tagttctgaa aagaagaaaa
    24481 attatcaatt cacgcttcaa ccatcgcaaa gaaagaaact atctaaaatc agcgaaaagt
    24541 taggaattcg atcagattca gctttacttg gtgaaatgat agataacttg tatgatgaag
    24601 tttttggtga gtgatcagtt taaagctgat cacttttttt attggaataa gcggttaggt
    24661 atagcttaac taaatttaat atacgtgttt atggatatat acgtatacat gtttatatac
    24721 atgtatacgt atatgtttat gtttatattt gtacataata aaaaggctag gtattgtaac
    24781 ctcgcctttt tatttttaaa gtaagtcttt gttacttggt gcattgaacc ctttcagcat
    24841 tggcttttct ttctttgctg aatgatcgga tgtaaccgtt gaaggatgct cttgttgaat
    24901 agggttatga tcagttggtt tattctttac ttctgctact ttgctatcgt ttctatcgtt
    24961 gcctgcttca ataattgtaa cttcattgtt ctttacacgc cgatataggt tcaaagcttt
    25021 tttgatcgtc tgagagtatt taacgcctag tgtttcatat tcggattcaa gaaactctgc
    25081 taaaggtaac tcgctaacct tttctctatt gattataact acataacgct ctgattttcc
    25141 cttattcatg ttgatctcct tcgctgtttt attgagttaa accaaacttg taaaagcctt
    25201 ttacattaga aaattcactg tcttcaacaa acaaggtatg cggaatttta tcttttacaa
    25261 gttgtttgtc gataatagaa gctccaccac cagtaaagac aactttatct aaggttgtga
    25321 agtttttgaa tgtagctaaa acttctgcaa caaccatatt ggtgtatgtt tttatttctc
    25381 tttcaactac ttctgaaata tcaaaacttt catttccgct tggcttaaat agatatacgc
    25441 ctttttcttt gccttctcgg ataatctttt caagttggaa agcattagga ctgatcggaa
    25501 ggtcaagcat atttacgata cgattatata aagtaaaaat gccggtatcc ttttgttcac
    25561 gattgttttc gtcaatttca aaattttgga caacatcaaa aagtttcgtg ccaccgccaa
    25621 tatcaacaaa accgatcttc tgactgatca atgtttcatc ttgtacacta ccatcatcag
    25681 caagtaggag gttatatagt gttccaatcg gctgtggcag tacccaaacg gtatctacgt
    25741 ttatattata agctttaccg tcaacttcaa ctgaatgttg tcgtgtggct tgttgcatca
    25801 cgtatttgac gattgtttcg gtatagtcgg atgaaggcag acctaaaact agattagcgg
    25861 ttaaaacgtc atcagattga tcttctaaac gtgctaggtt agctaaagag aaagatagga
    25921 tattttggta ggtttcctgc ttgtatctgc tgatcccaaa acctagtgat tctatcacgt
    25981 agtcatcttt tcctagctcg tgtatatcct tcccaaagta atagctttca gtagtgttta
    26041 ctaggctata ttcagaaacg tttagtgatc gttttaatga ggaacctata ttagaaaaac
    26101 gtcctgaatt aatgatattt ttgttcaaaa tacgagaggg aagtgagagt ggagaaacgt
    26161 gtgtactcat ttcttttact gatttattcc ctaagtcaac ggaaaacaat aatggttctt
    26221 tcatggtatg acctctttct gtttgtgttt atggtatttc ggtacgaaaa tagtttacac
    26281 ctatttacta ttagttgcaa ctgtttataa gaaaaaaatg caatcataaa agtctaatta
    26341 attataataa agtgtaaata aaaaggtgta aataaatgct tgcaaattta tcttattatg
    26401 atattataaa aacataaatt atattaagga gtgatcctga tgcgattgag aagaaataag
    26461 aattacacta ccattatttt agaagatttt actgatcaag aattacttat tgtcggtaaa
    26521 acaaaagcag attttatcga aaagggaaat gcttcaatag taaaagcttc aaagattgct
    26581 gtcgaaaaat attttgattc tttagaagaa agtgctaagg aaatttttaa tggagagaaa
    26641 gctggtagct atgagttaga cggtattaga tttagcttga tcccaacaac tgaaaatatt
    26701 attccgaaga aattatatta tatcttttca tctgaaaaac atacttcaat gaagccttgt
    26761 atttttgatt ggcaatggtt gattgatgct gtattggatg taaaatgttc aaaatacaat
    26821 attactttgg ggtgttaatt atgagtatgc tagttagaaa attaccatta tctgtaagag
    26881 aatttctatt ttacttggtc tgcttaatcg catcagggtt taagctgatc gaacatattt
    26941 ttatctttgc ttatgtatta gcaatgatcg catttttatc tggcggttgg ttaattattt
    27001 atttgttaag ctaagtttta gatcaaaaat aattaataaa ttaaacaata aaataaccgt
    27061 taataaatgt aatgaaagga caacgcctaa caatcagctt gtaataagat tataaaataa
    27121 cattatatta aaataagtgc tttttaaagg acaaattaag tgaatcaaca taaacgtgta
    27181 tatgtatatg tttatgttga tacttttttg tcttaatttt tttattttta aaattctatt
    27241 tactaatgct gtatagatag tgatcagaaa aaacaaaata gcgaggttaa gctatttatt
    27301 agttcatata ttatattcag attaatatga tccagtattg atctgaaggc aaaaccaata
    27361 tatttctacg gattcttgta aaactattta aattttcctg ctaataaaat aacaaaaata
    27421 gaatatttag cttttttata ccattttaca ataacatgat tttatttgta atattaaaac
    27481 atttaaattt tgattttttt tcaaattaga tcaattaata tccttataaa agagcataat
    27541 aaatgatttt tacatcgtta tttattgata ttaatctcat ccaaaaaaat gaaagcgcat
    27601 actttatggg tgattaaatg tgattttaat acatatacca attaaaagaa aactttttta
    27661 gatttttact tacaaattta taaaaaaaca agataattga tactttgatt ttgcgaacat
    27721 tttatctttc cgcctaattc agctgcaata tcatgattat atcagtaaca aatcgctttt
    27781 ttagctaatt taagcatcaa ataattattc ttaatcagtg aaaaaaaata acgaataaat
    27841 ggattttggg aataggtaaa aacttgatcg gtttctggat aaaaataaca tgaaaaagga
    27901 aatataaaat aacatggagc gtgggaggaa taaagaaatt ttacactcga ggtgcgtagg
    27961 aatgtaaaga aacttaacac gaaacaaaag t
//
LOCUS       DACDYJ010000035        27796 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDYJ010000035 DACDYJ010000000
VERSION     DACDYJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.035"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            236..1975
                     /gene="yfcC"
                     /locus_tag="IWM51_001542"
     CDS             236..1975
                     /gene="yfcC"
                     /locus_tag="IWM51_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9970968.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2132..3316
                     /gene="iadA"
                     /locus_tag="IWM51_001543"
     CDS             2132..3316
                     /gene="iadA"
                     /locus_tag="IWM51_001543"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP9970969.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3427..3630
                     /locus_tag="IWM51_001544"
     CDS             3427..3630
                     /locus_tag="IWM51_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9970970.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3731..4393)
                     /locus_tag="IWM51_001545"
     CDS             complement(3731..4393)
                     /locus_tag="IWM51_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9970971.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4406..5908)
                     /locus_tag="IWM51_001546"
     CDS             complement(4406..5908)
                     /locus_tag="IWM51_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9970972.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6456..8135
                     /locus_tag="IWM51_001547"
     CDS             6456..8135
                     /locus_tag="IWM51_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9970973.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8262..8741
                     /locus_tag="IWM51_001548"
     CDS             8262..8741
                     /locus_tag="IWM51_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP9970974.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8960..9640
                     /locus_tag="IWM51_001549"
     CDS             8960..9640
                     /locus_tag="IWM51_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP9970975.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9648..10979
                     /locus_tag="IWM51_001550"
     CDS             9648..10979
                     /locus_tag="IWM51_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9970976.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10994..11956
                     /locus_tag="IWM51_001551"
     CDS             10994..11956
                     /locus_tag="IWM51_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP9970977.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12067..12405
                     /locus_tag="IWM51_001552"
     CDS             12067..12405
                     /locus_tag="IWM51_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP9970978.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12484..13191
                     /locus_tag="IWM51_001553"
     CDS             12484..13191
                     /locus_tag="IWM51_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP9970979.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13294..14775)
                     /locus_tag="IWM51_001554"
     CDS             complement(13294..14775)
                     /locus_tag="IWM51_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9970980.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            15084..15620
                     /locus_tag="IWM51_001555"
     CDS             15084..15620
                     /locus_tag="IWM51_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9970981.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            15698..17068
                     /locus_tag="IWM51_001556"
     CDS             15698..17068
                     /locus_tag="IWM51_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP9970982.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            17135..17470
                     /locus_tag="IWM51_001557"
     CDS             17135..17470
                     /locus_tag="IWM51_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970983.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(17551..18408)
                     /locus_tag="IWM51_001558"
     CDS             complement(17551..18408)
                     /locus_tag="IWM51_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP9970984.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            18515..19030
                     /gene="tadA"
                     /locus_tag="IWM51_001559"
     CDS             18515..19030
                     /gene="tadA"
                     /locus_tag="IWM51_001559"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP9970985.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            19053..19137
                     /gene="ffs"
                     /locus_tag="IWM51_001560"
     ncRNA           19053..19137
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWM51_001560"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            19347..20732
                     /locus_tag="IWM51_001561"
     CDS             19347..20732
                     /locus_tag="IWM51_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP9970986.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            20769..21446
                     /locus_tag="IWM51_001562"
     CDS             20769..21446
                     /locus_tag="IWM51_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP9970987.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            21454..22218
                     /locus_tag="IWM51_001563"
     CDS             21454..22218
                     /locus_tag="IWM51_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP9970988.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            22220..22879
                     /locus_tag="IWM51_001564"
     CDS             22220..22879
                     /locus_tag="IWM51_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP9970989.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(22909..23418)
                     /locus_tag="IWM51_001565"
     CDS             complement(22909..23418)
                     /locus_tag="IWM51_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP9970990.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(23530..24528)
                     /locus_tag="IWM51_001566"
     CDS             complement(23530..24528)
                     /locus_tag="IWM51_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9970991.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            24729..25313
                     /locus_tag="IWM51_001567"
     CDS             24729..25313
                     /locus_tag="IWM51_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9970992.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(25390..26565)
                     /locus_tag="IWM51_001568"
     CDS             complement(25390..26565)
                     /locus_tag="IWM51_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9970993.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            26647..27027
                     /locus_tag="IWM51_001569"
     CDS             26647..27027
                     /locus_tag="IWM51_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP9970994.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            27101..27463
                     /locus_tag="IWM51_001570"
     CDS             27101..27463
                     /locus_tag="IWM51_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9970995.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         9050 a         4687 c         5789 g         8270 t
ORIGIN      
        1 attttctcta atctaaaaat aaaaaaagaa ttttaaggaa aaacagagaa ataaaatact
       61 taatataaat ggaaagagaa aaaaataatc atacatagga gaaaaaaaac aaaatagaaa
      121 aaagtgattt tctaataaat tattcatgtg gaaatgtcat ttttactgta taattcattt
      181 gattatccat tttttattca tcaaaattac tgaaaaaaga aaggaagaaa taaatttgaa
      241 gaaaacaaat tcttcaggaa aaccgaaacg cgacttcact aaattatcga caccccatac
      301 gtatgtcatt atttttggtg tagtcatctt tgcatggata ctgacttttg tcgttccagc
      361 aggaaagttc agcacgcaag atattgaata caaagatgca aatggcgaaa caagtactcg
      421 tactgtttta cgtcaagatt ccttccgtta tgcttacgag ctggataagt cgtatgtgtt
      481 tgatcaatta gaagaactgc aagatcatcc agcagaacgt gaaaaactgg atgttccgga
      541 aaaaggattg gaaaaagtca ttgctgacgg agaaaaaaat cttacgcagg aaaaactaga
      601 tgaaatctcc ttgacagatg atgtgcttta cgatcagtac ggcgaaaata tctatgatac
      661 ttctaaaaaa ttgcataaaa cagccaaaat atggggaaca gatgattttg gcggctttgg
      721 tttcttaaat tttgtttttg aaggactagt atctggagat aagtatggat ctgctgtagg
      781 gatcgctgca ttgatccttg ttgttggagg tgcttttggt attattatgc gtaccggagc
      841 gatcgacgca ggtatctatg cgtttatcag taaaactaag ggactggagc gtttagcttt
      901 accgctatta ttctttgctt tttcattcgg tggcgctaca ttcgggatgg cagaagaggt
      961 aattccattc tcaatggtga tggttccatt tgtcattgcg ttaggatatg actcgatcgt
     1021 agcagtaacc gtgacttatg tcgcatctca agtcggaaat gcgacctctt ggatgagccc
     1081 gtttagcgta gcggtggctc aagggattgc tggaatcccc gtgttgtctg gtgcgacatt
     1141 ccgattgatt atgtgggtag ttgttactgc attagccgct ggttatatga tgatatatgc
     1201 agaaaaaatc cgtaaaaaac cagaaagttc gttgacttac aaatcagatg attatttccg
     1261 ttctcatatc aaaaagacat ctgatgaaaa taaaaaattc atgcttgggc ataaattgat
     1321 cttattagag atgttagttg tccttgtctg gatcgtttgg ggcgtgactc aaaaaggcta
     1381 ttacattcca gaaatcgctt ctcaattctt tgtgatggga ttagttgctg gaatcattgc
     1441 tgttcttttc aaattagatg gaatgaaagt caatgacatc gcttcttctt tccaatctgg
     1501 tgcagctgat ttagctggta cagcaatcgt agtaggtatg gccaaaggga ttcttcttgt
     1561 cttgggagga tctgatgcat ctgttccttc agctttgaat acgattttgc atgggatcgg
     1621 gacggcgttg accggtgttc cagctgtcat tggtgcttgg gcgatgtaca tcttccaaag
     1681 tttgttcaac ttggttgtta catcaaattc aggacaagca gctttgacta tgccaatcat
     1741 ggctccactg gcagatttag taggtgtatc cagacaagta gcagttcttg cttatcagct
     1801 tggttcaggt tttatggacg cttttacacc cgtatcagcc agcttgattg gtgtgctagg
     1861 tgttgcacgg atcgaatggg caaaatgggc aagattccaa atcaagatgc aaggattcct
     1921 gtttgtttta ggaactattt tcatcatgat tgccattgca ataggtctgc agtagtactg
     1981 gagacctgta aataaaaaag taatccaaac agtagataag gactccaaga accgtcattt
     2041 tacggtaaaa tcaaaggagt aaagatccta tcgtttggat tattttttaa gatcctatca
     2101 tcagttatac ttaaagaaag aggaaaaatg tatgaaaatc attcgacaaa ttgaagtgtt
     2161 tgcaccagat tacttaggaa aaatggatgt actgattgcc ggtgggaaga ttttggctgt
     2221 agaagaacag ctagaaggcg gctacgaagg agtggaagta gaagaacttt caggtgaggg
     2281 aaaagtacta accccgggtt tcattgactg tcatttccat attttaggtg gaggaggaga
     2341 aggcggttat caaaatcgaa cgccagaagt cacattgagc caattgacta cagctggtgt
     2401 aacgacagtt gtcggttgtt tgggaacaga cggagaaggt agagatatga ctgcgttgat
     2461 cagcaaagcg aaaggtctgg aagcagaagg aatctccact tatgtatacg aaggttcgta
     2521 ccgattgccg gtaaaaacag taacagattc tatcatcaaa gactttttga caattgataa
     2581 aattatcgga atcggagaaa tcgcagtatc cgatcaccgt tcttctcagc caagttttga
     2641 agagttcgct cgtgcagtag cagatgctcg tgtaggaggc atgttatccg gaaaagctgg
     2701 gatcatcaat gtccacttag gcggaggaaa aagaaagcta gagctattga cacgaatcgt
     2761 agaagaaaca gaaattccga tcacacaatt tttgcctact catgcaaatc gcacaccaga
     2821 attatttgaa gcttgcgtgg catttgccaa acgtggtggc acgattgact tcactgctag
     2881 cgaagatcct gatttttggg agaagacaga tggtgaagtt cgcttcagca aagcactgaa
     2941 acgattgatc gaggaagatg tttctttaga taatttcacg atgacctcag atggacaagg
     3001 aagcttgcct tattttgatg aaaacaatca tttcctaggg ttaggagtag gaagtgccaa
     3061 ggcattgcta gtagggatca aagaagctgt gcaaaaagaa agtatccctt tggaaatcgc
     3121 cttacgagct ataaccagta atccagcacg tatattgaaa ttggataaaa aaggaaaaat
     3181 cgaaattggt gctgatgcag atctgtgtat cttggataaa gaaacgcttg atattgatac
     3241 agttatcgct aaaggagaaa taatggtcca agaaaaagaa gtcaaagtat gggggacttt
     3301 cgaaaaaagt ttttaatcaa cagctctaac tatctttttt agaaaacgag cgttctgttt
     3361 gtctgtatct gttgttgatg ttaccctgaa ggtagatcaa taaacaaact aagaggagga
     3421 agcacaatgg cagatttaaa aggacgttct aaagatatga aagataaagt agtcggtgaa
     3481 ggaaaagaag catacggcaa agcaacagac gacaagagca aagaaacaga agggaaagca
     3541 caatcactag gtgctgacat caaagaaaaa gctcgaaatt taggcgacga agtcaaagac
     3601 aagttttctc gaaaagacga aaaaaaataa aaaccactta ttactaattg aatcaagcaa
     3661 ataaaaaatc cgaaccatat ctgcgagaca gatatggttc ggatttttta tttgatcatt
     3721 atcctgtttt ttaaacttcg attttttgaa attttttatt ttttagttca aagacttcat
     3781 cacagctttt tgctacttca atctcatgag tcacgatgat aatacagcga ttttgttcat
     3841 gggcgatttc ttggaaaagt ttgacgatat cttgtgaagt tgtttcatct agatttccag
     3901 taggctcatc cgctacgatt agttgatgtt cacagcagat agcacgaacg atagcgactc
     3961 gctgctgctg acctcccgaa agtttggtca cctgtttttt tgctaatatt tcatcaattc
     4021 ctacacgagc cagtcgatct aaagcaactt gtcgataatt ctcttgcttg gtcttagtga
     4081 tttccattgc tgctatgacg ttgtctagag ccgataaata aggaagcaag ttgtaagctt
     4141 ggaaaataat cgagacatca tttttacgat aacttcttaa cccgattttg ttcagtgaac
     4201 gatcttgata gtataccttt ccttcttttg gtgcatctag tccagaaata agcgaaagaa
     4261 aagtcgtttt tccagaacca cttgctccta aaatagcgta catttttcca gcttcgaaat
     4321 tcagttctac atctttataa agcgcatctt gcggatcttg ataccaaaat cccaattgtt
     4381 ctgtccgtaa catcaaattc ctcctttagt ttaataggat ttttcttggg tttaatctta
     4441 atatccctat agaagctaaa aagacagaaa acagactgat tccaaatgct actccggcta
     4501 atagtcccaa ttgtgcaggt ttgacattga tttccaattc gctgacttga tcagaaacag
     4561 caaatggatt attcgtggtt ccttgttgac catcgcgttg accgccttgt tcttgtctgt
     4621 tacctggcat ttgtccaccg ccaggagctt gctggttttg tgtcgtggtt tctgtctgtt
     4681 ggtcaagtag ctgctgtccc acggcattcg cgacgacgtt tccgctaagc gaagcaactc
     4741 ctaatgcaag aatcatacaa atagccactt cggtaaacaa ttggagaatg attttacttc
     4801 tggattctcc taaagatagc aagacgccta actcatgccg acgctcccga atggtaatca
     4861 taatgatcag agtaagtatg ataataccgg ctacagccac tagtaaaaca acatttttag
     4921 aaaaacttgc cacgttgttt aaaggttcca acattgactg atacatggaa tcatttgatt
     4981 gtaagctaaa tgtatctgtg tcgatcagct tctcagcttc tttgagaaac tcatccattt
     5041 tatctggatc agatagagtg taaatagcag aatctatcgt gttatcgtca gatgtcccat
     5101 ttagttcatt tgcaaaagtg taagaagtgt aaagtgtatt cgacggattc atgaagttga
     5161 atttcatacc catactgccg cttgtttcgc tggattcata aattcctttg atcgtcattt
     5221 cataagtggt atcttcatct tcagtactcg tgattgtgaa agtatctccg actgccaggt
     5281 catttgtttc tgctagagta gaatcaatga ctacattgtt tgtgcctttg tcgctgtcat
     5341 ctattccttc accatccgtg atttttgctg ttccatcaga aaaattacta ttttgtgcag
     5401 ttgctgacac accactgatt tgaaagtctg cttgagtcat ttgcggattc ccgcccattc
     5461 ctccagcagg ttcaccgcct gcatcagtag tttctgaact gtcttcactt gaatcagaac
     5521 tagtgatggc agttattcca tcttttgcca tagctgaggc ggaactttca aaggaataac
     5581 tctttacatt atctaattca gctatttttt ccgcgtccgt taaggaaact ggtgtaagag
     5641 aaaaactgcc tggatctgga cgttcttcag tggtagaacc gcttgaagaa gtttgttcct
     5701 gttttttaaa tgaagcttct cgattggcac ttaaagtcac tgttgctcca acttcttttt
     5761 gtgcttgatt cgctgcttct tctgcagcgc ttcgaatcgt gaggccagct agcacaaaaa
     5821 taagaatggc tgtgaacacg gcaaacaata acaatgaacg tccccatttt acttttgtac
     5881 ttttccatgc acgtttcata aaattcatat tttgcatccc tcctagtaga tgcatttgat
     5941 aatagacaac ctatcttaaa gctagcttaa atcttatgct gtttgcacac catcaacatg
     6001 aggttctgta tatataaaaa gtcactatag ttattgcaaa aaatgtggta aaatgaaaaa
     6061 agtatgtttc aaaccgacga agaagaaatg aaagaggaat agcaaaagaa atcctagatg
     6121 ctacagaggg atttttacgt gcatagttgt tctaacaatt gctgatacta gcgatttagt
     6181 ttatttgttg tataataatg gggaacagct gaatacttat ataagtattc tggctatagc
     6241 gtttcgcaaa agatccattt tgttaatgaa gtatatccaa aataaaatgc ccatccaact
     6301 gtcagaacgc ggatgtgctg gtaattttcc tgctgattct ccataagttg agaaatagtg
     6361 attttgcata tgcttcattg gaatggctca tatatttaga taaagaagaa aatcgacatc
     6421 tttaaacttg gatgtcataa gaagaggtga agtttgtgag taccattaaa attatcccat
     6481 taggcggcgt tcgtgaaaat ggaaaaaaca tgtatgtcgc agaagtgggc gaggatattt
     6541 ttattttaga ttgtgggcta aaatatcctg aaaatgagtt gttggggatc gacgtcgtaa
     6601 tccctgattt tacgtattta gaagaaaaca ttgatcgtgt agctggtgtc tttttaacac
     6661 atggacatgc agatgcgatc ggggcacttc cttacttgtt gtcaaaaatt tctgtaccag
     6721 tattcgggac agagttgacg atcgagttag caaaattgaa tgtcagtcgt aatgaagcag
     6781 ccaaaggatt taaggatttc catgtggtag atgagcatac agaaatagac tttggtgaaa
     6841 cagtagtgag ctttttccgt accactcata cgataccaga ttcagtgggg atcaatctga
     6901 aaacaaaaga aggtagtatc gtttatacgg gagattttaa atttgaccaa acggctatcc
     6961 caatgtatca aacagattat gcacgattgg cggaaatcgg caaagaagga gtcttagcct
     7021 tactaagtga atcggcaaat gcagaaaatc ctcagcctgt agcttctgaa gcccaaatag
     7081 cagatgaagt atttgatacg atcaaatatt gggaagggcg tatcatcgtc gcttgtgtag
     7141 caagtaattt gcaacgagtg caacaagtat tggatgcagc agaaaaagca caacgaaaag
     7201 ttgtcttgac tggtcaagat ttcgaacgaa ttattcgcac agcgatgcgt ttagaaaaac
     7261 ttcaattacc aagtgaagat ctgcttgtta cgttgaaaga catgaaaaac tatgatccag
     7321 aagaactgat cattttagaa actggccgaa tgggtgaacc catcaagtct ctgcaaaaaa
     7381 tggcaaacgg tacacaccgt aatgtcaaga ttgaagaagg agatttggtt tatatcacta
     7441 ctacgccaag catcgcgatg gaaaccatcg tggcgaaaac ggaagatatc atttatcgag
     7501 ctggtggaac agtgaagttg atctctgaga atatgcgcgt ttctggccat gccaatccta
     7561 atgacttgca attaatgatc aatttgatga aaccaacata ttttgtgcca gttcaaggag
     7621 agtatcgtga attagctgct catgcagatt tggctcatgc agtagggatg ccatacaaaa
     7681 atatctatat tactggacgc ggagacgtgc tggaatataa gaacaagcgt atgagtgttg
     7741 caggaactac aagtgcagaa aatgtaatga ttgatggatt aggcataggt gatatcggaa
     7801 acattgtttt acgtgatcga aaaatcttgt cagaagacgg gattttcgta gcggttgtca
     7861 ctatcagtcg gcgagaaaag aaaatcattt caaaacctca aattacttca cggggattcg
     7921 tttacgtaaa agctagccgt gatctgatga aagaaagcag caatatgatt gaaacaatcg
     7981 ttgagaaaca tttagaaagc aatgattttg agtggagcaa attaaagcaa gacatccgag
     8041 atcaattagg ccgttactta tttgaacaaa caaaacgccg cccagtgatc ttgccagtca
     8101 ttatggaagc tactcaacgt cgaaataaaa aataaacaaa aagagtaaga tgaacctctc
     8161 tatagagtgc tcgtcttgct ctttttttgc gaaaaacaaa taatttttga atacagcatc
     8221 cctcgcttta taataaacga tagaataaag ggagtgaatc aatgatgagt caaacaaaag
     8281 tcgaacgtta tttagaagaa caaaagatca gctatcaacc acttgatttt agtcatgtgg
     8341 tagaagaatc tttcacagaa gaactacaaa aaagagggat tgatccagct ttgatatgta
     8401 aaactctcgt agtgaaagga aacaaaactg gggtgatcat cgcattgatt cctttgaacg
     8461 atcacttaga ttacaaaaaa acgagacaag tgacgaaaga tcgtaaagtt ggttttcctg
     8521 gaatggattt tgtattaatg catacaggat atccccacgg agcaaacacc cctattggaa
     8581 taaaactcgc acatcctgat tattgttttt tagcagacag ttctttaaaa gaaaaagaag
     8641 aagtaattgt gtcaagcggt gaaatcggga gaagccttcg gataaaagta gccgatttag
     8701 aaaagatcgt ccatcctatt tacgctgatt taattagtta agggacgaga agagaagcta
     8761 agcagagcag agacctcaaa cttgagatta tatctgcttg gtacttggtg catcctgtat
     8821 tatctgttac aattgctttg tacatactgt actatcaatc caatgaaaat atatttagca
     8881 gctatatttt ttcattgatg tgcaagtgat gaaatcgttt ttaataaaaa agatacagag
     8941 aaaacgcgag gtgtaaaata tgaaaattac gtatcatgga cattcctgtt taacaattga
     9001 aatggaaaat ggccaacgcg tgatgattga cccgtttatt acaggaaatc cattgacaga
     9061 cttaactgca gaagaagtaa agacagattg gttgctgatt actcatggtc atagtgacca
     9121 cataggagat atgattccta ttgcaaaaga aaatcaagcc acaattatca gtattgttga
     9181 gattgctgat tatgcgaaca gcagaggagt agattcattc ggtatgaata tcggtggaaa
     9241 acatgcgttt ccattcggcg tggtaaaatt cgtacatgca cagcatagtt caagctatga
     9301 agtagacggt atcgttcagt atatgggcga gcctagcgga attattattc aagcagaagg
     9361 aaaaacgatt tatcatgcag gggatactgc ttatttcagt gatctaggat tgcttgcaga
     9421 agaattcgat attgatgtcg ctttcttgcc gattggcgat aactatacga tggggccaga
     9481 agatgcagca aaggctgcgc aaacaattcg tgccaagaaa gtagttccaa tccattacaa
     9541 tactttccct gtcattgaac aagatcccga atcttttgta gagttactgg aagatagtga
     9601 aggaaaaata atgagcgtct cggaaacgct ggaagtgtag gcagaaaatg gcgacaaaac
     9661 atgatttgat tttagaatat attgaaagtt tacctgttgg caatcgaatt tctgtaagaa
     9721 gcatcgcaaa agaattgaat gtcagtgaag gaacagcgta ccgtgcaatc aaagatgcag
     9781 aaaatgtcgg actagtttca acaattcaac gagtagggac catccgtatc gaacgcaaat
     9841 taaaaaaaca tattgaacgt ttgacatttg gcgaggtcgt caggatcatt gaaggggacg
     9901 ttttaggcgg ggcggcagga ttagacaagg tcttgaataa attcgtcatc ggcgcgatga
     9961 ctaaacatgc catggaacgc tatattacgc caggatcact aatgatcgtc ggcaaccggg
    10021 cggaagtcca aaaattagca ttggaagatg gcgccgcagt attgatcaca ggaggattca
    10081 atacgactgc tgagattgca aagctagctg atgatcttga aatgccgatc ttacgcacct
    10141 cttatgatac atttacagtc gcaaccatga tcaaccgagc gttaagcgac caattgatca
    10201 aaaaagatat catgcttgtc agtgatatct atatgccagc tgaaaagact cattatttgc
    10261 agcagacaga tacgatttct gattatcagc gtttgtcaga agaaacgcag cattcgcgtt
    10321 ttccagtcgt caaccgccat catcgtttga tggggattgt tacggcaaaa gatgttttag
    10381 ggaaatcgcc aaaccagatc atcgatcggg tgatgacgaa agaaccaatc agtgtaaaga
    10441 aaacaatgag tattgcctct gttagccatc aaatgatctg ggatgggcta gaagtcatgc
    10501 cggtcgtttc cgatgacttg acattggaag gcttgattac ccggcaagat gtaatgaaag
    10561 cgatgcagct tgtccagcga caaccgcaga ttgcagatac gatttcggat cagatatctg
    10621 gaaatatcca tacgattgat actgaccgtg aaggaaatcg tttggaaaac cctaagttca
    10681 aattcgtagt agcaccacaa atggtcaatg gcgttggaac catttctttt ggcgtactta
    10741 gcgaaatcat ttctgatgtc gcacaaaaaa caatggtgat gaaccaaaaa cgaaatatct
    10801 tgattgaaca agtgaaccta cattatttgc ggctgatcca attggaaagc gaattagaca
    10861 ttcgtccacg tgtattggaa atcggcagac gttccgctaa actggatatc gaagtgttta
    10921 ttgaaaacac catcgtttcc aaagcaatcg ttgtttgtca agtaatggaa cgttcttaga
    10981 aagcaggagt gaaatgacag tacagcaaga aatagtagat acaataaaaa aatacgataa
    11041 aatcattatc cacaggcatt tgcgccctga tcctgatgca ttaggatcac aagtaggatt
    11101 agccgaacta ttacgggaaa gtttcccaga aaaagaaata cgtcaagtag gtgaaacgat
    11161 tgaagggctt cgctttttag cagaaatgca agaagttgaa gatgtcttct accaaggagc
    11221 gctagtcatc gtgactgata cagctaattc acccagaatc agtgatcaaa gatatcagtt
    11281 ggcagaaaaa ctgatcaaaa tcgaccatca tcccaatgat gacccttatg gagatcttgt
    11341 gtgggtaaat acaaaagcaa gtagctgtag tgagatgatt gcagaatttg ctttgatgtt
    11401 tcctgaatct ttaaagatga acgttaaggc tgcacggtta ctttatgcag gaatcgtcgg
    11461 agatacaggg cgctttttgt atccctccac gacgtcccgt acattagaga ttgcttcgat
    11521 attgagaaat tatccatttg atgcaagtgc tttgaacaga gagattgaac aaatgccgat
    11581 gaaagtcgct aaactttccg gttatcttta tcaaaatatc caggtggatg aaaatggtgc
    11641 aggaagaata atccttccgc aagaaattct tgataaatat ggaatcgatg attcagagac
    11701 agcttcgatc gtttcacttc ctggagtgat tgacgaagtg ctggcttggg ggatttttgt
    11761 tcagcagcct tcagggtatt accgcgtacg tttacgttct aaggggccga tcatcaatga
    11821 attggctaaa cgccatcatg gaggaggcca tccattagca agtggtgcga atgcaaaaga
    11881 tttagcagaa gtagaggaaa tctataaaga gatccaagat atatgccaat cgtataagag
    11941 cagaaaaaca gaatgatact tcgttcgaaa acgtgccttt tgtagtaaaa atcacaaatg
    12001 tatcttttca agatcttctt tttccgttac aatagtgctc actaaagatt ttaaaggaga
    12061 aagaagatga cattaccaaa ttgcccagaa tgtggctcag agtacacata tgaagatcga
    12121 ggcttgttga tttgcccaga atgcgggaac gaatggaaca aagaagaaca agaaactgtc
    12181 atagaagacg gattagtggt attagatgca aatggaaacc gtctgttaga gggcgatagc
    12241 gtgacagtga ttaaagactt gaaagtaaaa ggtgcgtcag gtgccattaa acaaggaaca
    12301 aaagtcaaga acatccgttt agtagaaggc gatcataata tcgattgcaa ggtagaagga
    12361 tttggaccga tgaaactgaa atcggaattt gtgaaaaaga attaaagaat attcaaggtc
    12421 ttttcacatt cttttcctac tactaatgat aaaataaagt aaacgaaaag gagtgaaacg
    12481 aaaatgtatg gttatggaat gtatggtttt ttagatccga catttctttt ggtcattctt
    12541 ggattagtta tttccggaat tgcgtcagcg tacgtcaata gtactttccg aaaatacgat
    12601 caagtgagaa gcaaaaacaa tgtgactggt acacaagcag cacaatatat attacaaagt
    12661 caacagataa ataatgtcgg tgttcaacaa attgccggag atctcacaga taactacaat
    12721 tcaggtaata agatgttgag tttgtctcaa gcaactgcac agtctacttc agttgctgcg
    12781 ataggtgtag cagctcatga atgcggacat gcggtacaag atgcttcggg ttatgtccca
    12841 ttgaagattc gtgcagcact tgttccagca gctaactttg gttcgatgat ttcgttcccc
    12901 ttgattatgg taggtgtatt gcttagctgg aactctacgt taatcaatat cgggattttc
    12961 gctttttctt tagcattact tttccaattg gtgacattac cagtcgaatt taacgcgtct
    13021 cggcgagcaa tccaaatttt gagtgaaggc ggactactga cggaagaaga agtaccgatg
    13081 gcaagacacg tattatttgc agcagcactg acttatgttg cagcagcgct ttcgacattc
    13141 ctgcagctgt tgcgcttgat cttattgttc ggcggcaacc gaagagatta aaataaagaa
    13201 gttgtgaaat cagcgctttg atctgagcta ataccgagag ttagctgatt gaataccatt
    13261 tatttggaat aagaggatgt gacaagaatt ttgtcacatc ctctttgttt tgttctcata
    13321 accttttttc atatacagtt catgaaccgc ttttattaac ttgccaaata acactgtatt
    13381 gtgcgttgta tccagataga aagtctccgt ttcatctgaa attttctcat gctgatctgt
    13441 aatgatcaaa tcagcatgtt ctgaatatgc ggtaatttta acagcatctt tgcgaaataa
    13501 ctgctgcaaa tggctttgaa gatgataatt tcctgtaaac tgtttggtta tctgcaaatg
    13561 gatgagtaac tgctctttcg tttctgtttg tagtaagtgt tggatcaatt gactaaggta
    13621 ttgactgatt tggtttcttt ccccattttt tgaaaaaatt gtatcgattt tttctcttaa
    13681 tttcttttcc tgtataaaag aactagaatg attggacgtg gcttctggaa agcaaagtgt
    13741 agaaaaacta ctgctggatt cacccaacag ttcgaaaaaa acaactgcca acacaagaaa
    13801 ataaaaataa attggtattt tattttctaa atggttgaga ggaaactgtt gagttaagta
    13861 agtcatcaat tgaccgactt gtcgacaaat aaggtttttg cttgtggaaa ataaaaaacc
    13921 aatctcctgt ttttgtcgaa aagagatttg atttggaaaa aaacaataga aaaaataatt
    13981 aaagtaggct ctctctgttt ctgtctcttc acaagggcga ctcactgaaa aagaagtaaa
    14041 gtattctgaa taatcatgtg tatgctgcaa taagtccatc aattcagtga tttcagaaga
    14101 agtttttgga aaatatgcct gttgtttgtt tcgtaatatg aataaagtta acagcaagta
    14161 gatatttttt tcttgttttg aaattttttc tatttgctca tctgtactgt tcatttttag
    14221 tgcatttaat tcaagtgagg aaaatggcca atcgttacct ccaaatgtat ctgaaagaaa
    14281 aaagtaaaga tagagacgga tagataactc atttccttca agagtacagc aattttttac
    14341 agataggatc cgtaaatgga atttttttaa ggaatgattg agctgttttg tcagtttttc
    14401 gagataggag ggcgatatat aaagttgttc aagtaaatat ttttttggta cagcagtttg
    14461 tggataattg attagtaaca tcaaatagtt aaaacgattg gattctttta ggtagcataa
    14521 ttttaactta gaaaaaacag ctaacttttt agtttgatcg gtttctttgg tttggatctc
    14581 tcctttatga ttagaataaa taaattcatc aatcaataaa aattgcttga gtgatacatt
    14641 tatttttcgg atcagttctt tgattgtccg ttttggaata tgtaagtcat tggataattc
    14701 ttgtaaagaa caattatttt tttgaatgac catttgtaga acaagtaact ctttttttct
    14761 tgcttcaatt agcataaata cctccttttt attctgtttt tgtatatagt tagacaaaaa
    14821 ctgtgagtgg aaagtcattt cagtctgtgt tattctcaca gagtgtcgcc ttctttttta
    14881 tataacgtta tttaattttt ttgttacctt gtttattcta ttgtttattt cctagggtat
    14941 tttgtcaaga tattcttgtt tttaaggcgg aaaaattgat ttttaatgga tataactaaa
    15001 tatgttttga taattatcaa taatattttt agaattattt atgttattta tttatgggta
    15061 gatgaaaaga gacggaggta agaatgatgg ggagagtaac aaattatagt aaagaatatt
    15121 tgctgttcaa aagtatggta tacgtggaag aatatggaat gaagtcaatt actgcaagag
    15181 agttagcaga gttctgcggt tgttctacat acccgattta tacacatttc aaatcaatca
    15241 gcgggttaaa agagaagata ttagaagaaa tcactgttta ttttgaaaac tacttagctg
    15301 aatgcagttc cgatgatgtt ttaagcattg tctacttatt gaaagatttt ttttctatcc
    15361 atgaaagtat tcgtgaaatc attgcaaaat ctgatttaga catggattcg ctatttaagc
    15421 ggccattttt ctcatatgtc aaaggtcaca cgaagatcaa agaagaaaag tcgcttgaat
    15481 tggtatggct taatgcattg ggaagtttgc gctcagatga ttcttctcaa gattttcttg
    15541 aaatgatgaa cgctgtttgg gataaaaacg aggtgacgga tgaatacttg gaaaatatct
    15601 taaaggaaca gtcaccttag gcagcaacat gaaaaagtag atcagccacc tttatttttc
    15661 gtaaaaaaga ttgtaaaatg tctaacgaaa cattggaatg aaggaggaag cgacaacgtt
    15721 accacagcaa tttatcaaaa aatatcgtct tcttttaggc gaagaagcat ccgatttttt
    15781 ttctgcgctt gaacaaggaa gcgtgaaaaa aggatttcgg tggaatccac taaaaccggc
    15841 aggattggac atggtccaaa cttaccatag cgaagaatta caacccgctc cctacagcaa
    15901 tgagggattt cttggcactg tcaatggaaa gtctttcctc catcaagctg ggtatgaata
    15961 tagccaagaa ccaagtgcaa tgatcgttgg aacagctgct gcagcaaaac ccggtgaaaa
    16021 agttttggat ttgtgtgcgg ctcccggtgg taaatcaacc caattagctg ctcaaatgaa
    16081 ggggaaaggg ttattagtga cgaatgagat ctttccgaaa cgtgcgaaga ttttatcaga
    16141 aaatatagaa cgatggggag tttcgaacgc aatagtgaca aatcatgcac ccgcagaact
    16201 tgtccctcat ttttcgggct tttttgatcg gatcgtggtt gatgctcctt gttcaggaga
    16261 aggaatgttc cgaaaagatc caaatgcaat aaaagagtgg acagaagaat ctccgctata
    16321 ctgtcaaaaa cgtcagcaag agattttgtc ttcagcaata aaaatgctaa aaaataaagg
    16381 acaactgatt tattcgactt gtacgtttgc accagaagaa aatgaagaga tcattagctg
    16441 gcttgtagaa aattatccag taacgattga ggagattcct ttgactcaat ccgttagttc
    16501 aggacgttct gaatggggaa gcgtagctgg tttggaaaaa acaatacgta tatggcctca
    16561 taaagatcag ggtgaaggac attttgttgc aaagctgact tttcatggac aaaatcagat
    16621 gcataaagaa aagaagacac gaaaaaaaag taaagtgcaa atgacaaaag agcaggaaaa
    16681 actctggacg gagttttcaa atgattttca ttatgaagct actggaagac ttttagtgtt
    16741 caacgaccat ctttgggaag ttcctgagtt agccccttct ctcgatggtt taaaagtcgt
    16801 acgtacaggt ttgcatttag gagatttcaa aaagaatcga tttgaaccta gctatgcatt
    16861 agcgcttgct acaaaaaaga tagagaatat accttgcttg ccgatcactc aaaaggaatg
    16921 gcaatcttac acagcaggag aaacatttca acgtgatggg aatcaaggat gggttttgct
    16981 agtattagat aagatacctg tcggatttgg gaaacaagta aaaggaacag taaagaactt
    17041 tttccctaaa ggattgagat ttcactgagt ggattagttc gtttattttt ttgtctatgc
    17101 tatgatggag atagataaaa aggagctgat acttatgaaa acatcaacaa aagtaacaat
    17161 cggattaagc gtagcagccg ctgccagtgt agcgacagca gtcgttgtat cgggaaaagt
    17221 aatggaaaag atccatcata tgactactcg gacgaaagta aaaaaattcg tctatgacaa
    17281 atttgatgga aatgaaaaat tactagaaat cgtagatggt ctttcagata gtgacttgga
    17341 ttctttgatg ggaatgttag caaaaatcaa atcaggaaaa aagaaaatct ctgtttatgg
    17401 tgattcaatc aaagattcaa ccgaagatgt aaaagaccgg ctaatgcatt ttgttgaaaa
    17461 aatgatgtaa gagaaactag cttttgaaca ctattcattc gaaataaggg gttgtgacaa
    17521 aattattgtc acaacccctt taatctatct ttaattatgt tcatgaagca ataaaagata
    17581 gcaaaacagt aaatcgttgg ctttttttag attgaaacca gactgttctt gaaatttttc
    17641 gattcgatat tttagagtat tacgatgaag aaacagtgtt tttgcagcag agctgacatt
    17701 gcctaattca ttccataatt cttgaatgat cacttccatt tcattttgtt tgatcagttc
    17761 tatgtaactt ttgatcaact tactatcttt tacagtatct tttgtatagt aatgtaaagc
    17821 cacatcttgc atagaaaaag tacggctgtg agtataaaga ttgttttgct ctgttaaaaa
    17881 aatttttctt tcttcagcaa acaaagggac gaggtcggtg ccaggtgaat ggaaagaacc
    17941 aacgaataca gacgttgtcg tatcaaaatc agcatctaaa gatagaaaaa taccgttgat
    18001 ttcagtagca tcaagataat cacggtttat ttgttcgact aagagcgcat ctgtgtctgt
    18061 ataaaaaaag aaatcagcag aatagcgaaa taattccaaa atagtatctt gccattcttt
    18121 ttttaagaat tctccttttg attcgacgtg aagctggatc aaacgaaagc ttttttcttg
    18181 aacacaagaa gcatctttaa aaagatagtg ataccatggg tgtttttttt gatcgttgat
    18241 tacagggaat aacgctttca aaaggctggt ttcttgttga gataatgaat cttgtttgat
    18301 ccagataaaa gcatcatcaa ttgcaaaaga aaggatatcc gcttctgatg ctggagagtt
    18361 ttttttctgt gcttgtgggt aaagagcaag taatttatcc attttcatct tgttcacctc
    18421 ttgtatttat tttaccatag aaaaaaaagt tagttacagc ctttttatca tatgctaaag
    18481 taaaacaaga aaaaacaaag gcagggaagg gtaattggta aatacaaatc ttgatgcagt
    18541 agaaaaagaa aaatggatgc gccttgcatt ggcagaagca aagaaagcag aaatgcttca
    18601 tgaagttccg attggagcag tggttgtatt ggatggaaaa gtgattggaa gaggatataa
    18661 ccttagagaa accacgcaag atgctactac acatgcagaa atgcttgcaa tcaaggaagc
    18721 ctgtgaaaaa gtgggaagct ggcgcttgga agatgctgca ctttttgtta ccttagagcc
    18781 ttgtcctatg tgcagcggcg cgatgatttt gtctcgtgta gcagaagttt atttcggcgc
    18841 gtatgatcca aaaggcggaa cagccggtac cttgatgaat cttttggagg acgagcggtt
    18901 taaccaccaa gcatatgtag aaggtggcat tttagaagac gaatgtggga tgattttgac
    18961 tgactttttc cgaaagctcc gccagcgaaa aaagaaatta aaaaaagact ggtcaacgaa
    19021 tgaaaaataa ggtatactat tctttgccga aaggctagga caatggtggg cttgtgaagc
    19081 gtgtcaggtc tggaaggaag cagcactaag caggttccgc catgtgtctg attataatga
    19141 aacgacaaca gactttcggg tctgtttttt ttatttttat agtactgtaa aaatgaatgt
    19201 tcctgatttt ttagccagac tgatttatcg aatattgtac ttatacaatt tgcttcaagg
    19261 caggaaaaac tctgaaatag agcttgtttc aatggaagaa aaagctgcct atggtatgat
    19321 aaagataaag agaggcgaga gggtacatgg aaaaaatagt gattttacat acgaatgatc
    19381 tgcattctca tttagaaaat tggccaagga tcagaaggtt tttggatcaa cgtaaacgag
    19441 aaaacgaaaa gaaagaaaaa acgacaacga ttgcagtaga tctaggtgat tttgtggatc
    19501 gttggcatcc attgtcagaa gcaacgaatg gacaggaaaa tatcttgctg atgaatgaag
    19561 ttggttatga tgcagcgacg ataggaaaca atgaaggcgt aggaaattcc aaagaagagt
    19621 tgaatcattt atacgatcat gctgagtttg atatcatcct ggataactta ttcgataaaa
    19681 atacgctcca gccaccggca tggacgaaac catataaaat cattacaacg aaacaccaga
    19741 cgaaaatcgg actaattgca tttactgctc catttccgct tacttataat ccgaatggct
    19801 gggacatacg gaatccttat gatattttgc cagaattagt cgaagattta cgttcacaag
    19861 tggatgtttt agttttgatg agtcatctcg ggattcagga tgatttccaa attgcaaaag
    19921 aaataccagc aattgatgtt attttaggat cgcacaccca tcatttattc agagaaggtc
    19981 acgtaatcaa tgatgtccaa atagcagccg ctggaaagta cgggcagtat gtcggtgaag
    20041 tccatttaac catcgatgaa gagaaaaaaa tccttaagca ttcagccaaa gccatcccta
    20101 ccgaaacaat gacagctttt acagaagatg aacaagaaat aaatagttat ctagaacgtg
    20161 ggcatgaatt attaaaagaa aaagaagtag cagatttgcc attttctctg agtctagata
    20221 tcttcaatga gcattcgttt atccaagtag cctttgaggc ggtgaaagaa cgaggaaaga
    20281 cggaagcagc aatattaaac agtggtctgt ttttaactga aattccaaaa gggcgggtca
    20341 atcaggatca gcttcacact gcgttgccgc atccgatgca cttgttaaat gtcacattgg
    20401 gtggaaatga tctgatccgc ttagttttag aaatagaaaa aaatcgtaac tttttacgta
    20461 actatcctat gaagggaatg ggatttcgcg gtaagatttt tgggcagatg gtctataatg
    20521 gaatcacata tgattctatt aaccatcaag tcttttggaa gaacaagcca atcgatctga
    20581 ataaaaatta tacttttact accgtagatc atttgatgtt tgttccattt tttccaacga
    20641 ttgagattgc tggaaaaaat gaatttttgt tccctgaatt tatccgaagt gtggtaggag
    20701 attacttgaa agctcactac ccaatcaaat aaaaacaagg tataataaaa aagataggtg
    20761 gtgaacgcat gtctgcaaaa caaaatgaac agcagaacgt tgagcataaa acagaaattg
    20821 ctaagaaaaa atcatcagct gaacaggaaa caacagtcac cgaagaactg acagctgctt
    20881 tggaagaaat cgttgaggag cctaaagcag aaaaagtcaa aggcgagcta gtgacactgt
    20941 tagatgacag taattttttg ataggtgaaa gacattatcg gcttgtgtcc gattatcgtg
    21001 aaggatttaa tgcagaaaag cttggagaac gttacagtga tgtactagcc cgatatgatt
    21061 atatcgtagg agattggggt tatgaacaac tacgcctaaa aggatttttc caagcggata
    21121 atcgacgggc gcatccagat cagcgaatcg attccttgga agactatctt tacgaatatt
    21181 gcaattttgg ttgtgcgtat tttgtcatcg agagaatcgg cggtaaaaaa gaaaagacaa
    21241 ctcagcgccg aaaaaagaaa aaaaaccata atcgtgcgta tatcgatgag aaaaaaggac
    21301 cagtcgcatc taatcgcaaa aaaccggtta ttaaaaatcg aaaaacagag tcagcaaaaa
    21361 cctctgaaaa agtaaaaaaa caagaacaac ctaaacaagt agaaaaaaca aaaacagcat
    21421 ttacgatcag aaaaagggaa gagtagtgga tgaatggact ataaaggata tttaattgat
    21481 ttagacggaa cgatttatcg tgggacacaa cccataccgg ccggtaaacg atttgtggaa
    21541 gaattgcaaa aaagaaaatt acctttcttg tttgttacca ataacacgac taagtcacca
    21601 gaaacagtag ctaatcgtct agcagatgag tttgacattc atgtagctcc agagacagtc
    21661 tacacagcta ctttggcaac aatcgatttt atgaaagcag atggaaaagg gaaaaaagtc
    21721 tatgttatcg gagaagcagg attgattgac ttgattttag ctgccggatt tacatgggaa
    21781 gaagaggctc ctgactatgt agtggtaggc ttagataatt atctgaccta tgaaaaagta
    21841 gttaaagcaa cacttgctat tcaaaaaggt gctactttca ttggaacgaa cccagataag
    21901 aatataccta ctgaacgcgg ccttcttcct ggagcagggt cagtcatctc ttttgtagag
    21961 acagcaaccc aaacgccgcc gatctacatc ggaaaaccag aagccatcat tatggataaa
    22021 gccgttgaag tattagggct gcaaaaagaa gaagtaatca tggttggaga taactatgaa
    22081 acagatatcc aagctgggat acgcaataat atagatactt tgctcgtttt atcaggtttt
    22141 acaaaagaag aagatgtacc tgggttgcct atcccagcta cctatgtgaa acaatcactg
    22201 gatgaatgga gcttctaata tgaaaaattc gagatggcag tggatggaat atgctggatt
    22261 gttcagccta tttctgacac tgatcagtct ggcagttggt gtgacgatca actttcgacc
    22321 actatacgta tttgatatcg gacatttgca aattttggat tatacttctt tagatcaaga
    22381 gacgttactg aaaaattttg atcacctaat gaattatctc aataatccct tcaaaaccat
    22441 cctcagcttg ccagatttcc cagtttctgc tagcggagcc catcactttt atgaagtgaa
    22501 aattttgttt ttagtggatt atgctgtgtt tttcatcact ttgatcccga gtattttgtt
    22561 tatcaaatat ttgcaaaaga atgatcggct ttggcggttg attcgtccgt ttcaaatcgg
    22621 catgttactg ccagttgttt tcggtttttt catgatgata ggatttgatc gcttttttat
    22681 cctttttcat gaaacgtttt tcaacaacga tgactggtta ttcgatcccg ttactgatcc
    22741 aatcatcaat gtacttccag aagaattttt catgcatagc tttatcttat tttttgtatt
    22801 attagaatta ttttttgctg tttttctttt cttaggaaaa aatagcttga aacaaacaaa
    22861 aaagaaagaa ctcgtctaaa tagacgtttt ctttcttttt ttgttgaatt attgagtaag
    22921 gcgacttctt ttttctggtt ctttgtgtgc taatcgactg gcagcagcag cggcgatcgc
    22981 tccgacgata tcatctaaga aggtatggac atgaacgcca tcgtggctgt ttagttcttt
    23041 taaaatacca ggtttcacct tgtcgatata accataattc gtaaagccga tcgtaccgta
    23101 aacattcacg attgacaagg ctaggatctc atcgataccg taaaggcctt cgtcatcttc
    23161 gataatatgt tgcaacggac tcatcaattt tttcttttct gctaatatat ctaattggat
    23221 tccagtgata atggcgttat gtacttcacg ctttgttagc acgctgttga cactttcagt
    23281 acaatccgcc aaagtcaaat cagaaatata gtctttttgt aaaaacatga caagttgtgc
    23341 gatatcttct acagttacgc cacgttcctt caatagttct cttgcctttt cttctaaagt
    23401 ctcagttttg acgaccatat tattgctccc cctttatttt ttctttctct atttcatgtt
    23461 aacggaacat gaaaaaaatg caagaaactg ctttcgggtt taacttgaaa tgcaaagaga
    23521 tataaaaaat caactaggac gaagattttt atcgtataag cttgtgctgt gaccaggctg
    23581 agttcgttcc tttggattaa tatagtgtaa agcgttgttc actgcagtag gtgcttcacc
    23641 gagcccagta gcaatcagtt tcacttttcc ttcataggta cagatgtcac cagctgcata
    23701 aacgcctgga atagaagtag acatatctga ttttacagta atggcatttc tggtactatc
    23761 aagtccccaa gaagaaagat gttctaaatt agaagagaat ccataattga cgatcagaga
    23821 atcaatcatc aaatcaatcg tctcgtcact tttcactttt tttaaccgga tatctgtcag
    23881 ttccccatta ttgcctgata atccatcgat gagataggga gtaagcaagt tgacagatga
    23941 agatttcaag cgtgatacac tatgttcgtg cgcacgaaat tccgggcgac gatgaatcaa
    24001 gtaaacttcg cttgcaatag gctctaacat caacgtccaa tcgatagcag aatcaccacc
    24061 tccagcaata gcgacttttt ttccagcata cttcatcaaa tcattcacga aataatccaa
    24121 tccgtggttt tcaaatgatt cagcattatc taaattcaat tttcttggct ggaatgaacc
    24181 atttcctaaa gctaaaatga ctgctttgga ataatggatg cctttatttg tagtaatctc
    24241 gataacttcg tcttcacgag taagcgagag aacttcttct tttaaatgga atgtatggtt
    24301 aaaagtagtt aattgttttt ctaattgatc gattaattca cttgctttta ttgctggata
    24361 cccaggaata tcgtaaatat atttttcggg atacaaagtt gctaattgac caccaagttg
    24421 aggtagactg tcaatgattt ttgttttagc ttggcgcatt cctgcgtaaa aagcggcaaa
    24481 catccctaca ggacctgcac cgatgattgt aatgtcatag atttccataa aaattcctcc
    24541 cttttcacac tatagcagaa tttaattgag aatgcttctc aaaaatgttt gaattcgaaa
    24601 tcgaaacggt aagttataga atgtatctag tgatgaaaaa taaaagaaaa caaatctagt
    24661 atttttacga gaggttaggt attatgttat tattattatg aagtatgcaa atattaggag
    24721 gaaacaatat ggcattccca caactagatt taaaaagtga aaaaggacca aaagctgtta
    24781 tcaaaacaaa ccgtggtgac attactgttc aattattccc tgaactagca ccgaagacgg
    24841 ttcaaaattt cattgaactt tccaaaaaag gttattatga tggcgtgatt tttcaccgcg
    24901 ttattcctga tttcatgatc caaggcggcg atccaactgg aacaggaatg ggtggcgaaa
    24961 gtatttatgg cgaaagtttt gaagatgaat tcagtcgtga actgttcaac cttcgcggag
    25021 ctttatctat ggcaaattca ggtccaaaca caaacggaag ccaattcttt attgtaaata
    25081 acacaaatgt gccagctaat atgcttggac aactagaagg tgctggattc ccatcagaaa
    25141 tcattgaagc gtataaaggt ggcggaactc cttggctaga tttccgacat acagtctttg
    25201 gacatgtact agaagggatg gacacagtag atgaaatcgc aaatgtacaa cgtggtccac
    25261 aagaccgacc tgtacatgat gtagtgattg aaacaattga aatttcagaa tagtttggta
    25321 tggtacggag taattatgaa aaaggacgaa acctagtaag gtctcgtcct tttattttga
    25381 ataaatgatt taatcgattg tctctaaagc atacttgatt cgattaagtc cttccagtaa
    25441 agtttcttct ggacaagcaa tattcaagcg catatgccca tgaccggaag gaccaaacgt
    25501 gatgccagga tttaatacga cttttccttt ccgaacaaga gtgttctcca attcttggtc
    25561 gtctgaaata taggcggtaa aatctaacca cattaagtaa gttccttcag ggttcattat
    25621 tctcactttc ggaagatact gttcaaaaaa ctgattaact gtttgaacat ttttttctag
    25681 ataaggaatc agttggctta accattctcc acccgtctca taagccgctt gggttccaat
    25741 cagtccaaaa gtgttgattt cgtgctgttg gttcatcact aaatgttttt caaaatcagc
    25801 tttcatctct ggatttttga taaataccat tgaatttttg attgcagcta gattgaaagt
    25861 cttggttgcg gaagtaaaag caactagtag atcatctaaa ttaggatcaa tcgttagcat
    25921 ggaagtaaat gtatgatcga ataaagttaa atcttgatgg atctcgtcac taaacagaat
    25981 cacttgatgt tttaaacaca attcgcttaa ctgtttcaat tcttctttgc tccaaacacg
    26041 ccctccagga ttgtgaggat tgcataaaat cattgctttt acattgtttt cttcgatttt
    26101 tttctccatg tcagacaagt ccatgatgaa atgattattt ttttctagca aagagcttcg
    26161 aacgatgtga cgttgatttg tttctataat agaggaaaat ggaggataaa ctgggtcatg
    26221 aatcaaaata gaatcgcctg gttttgtaaa agtttgtact gctgcagcta aactggttaa
    26281 cacaccactt gtaaaaacga tattttcttt cgtcaactcc acagcatggt gttggcgttc
    26341 ccaatgaatg atagcctgat atagtttatc agacagagtg gcataaccaa agattccatg
    26401 tttgatgtat tcactaaacg ctttagctac cccaccggga gacaagaagt ccatatctgc
    26461 tacccacaaa ggaagtaagt catccatttg atatgtctca gcaattgcgt cccattttac
    26521 actttctgta tcttttcttg aaatctgttt atcaaattcg atcatcttaa gacctccagt
    26581 cattttcgtc gcttcttttt ttgagctatt tacagtataa tgataaatgc gaagagaggg
    26641 aagtagatga cttataaaat tggacaaata attgaaggaa aagtgacagg gatacagcct
    26701 tatggcgcat tcgtttctct tgataacgag acgcaaggac ttattcatgt ttcagaagta
    26761 caatccggtt atacgaaaaa tatccattca ttgttaaaag tcggacagcc agtaacagtt
    26821 cagatcatcg acattgatga atattccaaa aaaatcagtt tatctttaag aacgttagaa
    26881 aaaacaaatc cagctgttcc atatcgcaga aaaaggtatt tcacgaataa aaatcgtaag
    26941 atcggattta ccactattgc ggagcagtta cctatttgga tagaagaagc attatctgaa
    27001 ctagaacgga aagaaaataa gaaataaaaa gtgtattttt tctgaaaatg tgctagaatt
    27061 tttatgtaag ccatagaaca agttgatcga ggtggaaatt ttggcaagca aaagagttgc
    27121 tggtacaatt atgttgcatt tggaggatgg tactaaaaaa tttttggtcc attcaattga
    27181 tgacaagttg gaatttgcgt tagcggaact ttctgaagga aagactggtt tagcgaatat
    27241 acttaatttc ttaaaagaaa tagttcatat cgatgttagc aagattagtt tgatggaatt
    27301 gacaaataca catattaaag aggaaaatgt tccgttgttt gtctttgaaa ctgaacaaaa
    27361 gaatcaaaga gaagaattag gcgaaggata tatttgggaa gaaccaggtc aaatgcgctc
    27421 tgttcttgga acttacgaag tagagggagt gcctttcttc taaaatacag attaagcgaa
    27481 caaagaagtt tgaatttttt tcaaacttct ttttttattg tttggtccaa aaaatacaat
    27541 gaaatgttct gctaaagata gttttttagt gcattttatt ttcaaaatat gttgaaaacg
    27601 gttgtataac gaataataat aattgatcga aaaatcattt cacaaagtaa tgcttaattt
    27661 atagttgacc aaggaagaaa tacatgataa atttaacagc gttgattctg aagtggatgt
    27721 tatatcacat ttctaaaaaa tttaaaaaat agttattgac atgttactaa cgacatgata
    27781 agataactga gtcgtt
//
LOCUS       DACDYJ010000036        26961 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDYJ010000036 DACDYJ010000000
VERSION     DACDYJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.036"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(136..2289)
                     /locus_tag="IWM51_001571"
     CDS             complement(136..2289)
                     /locus_tag="IWM51_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP9970996.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFAPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKALELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYTPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTAAKPLASAKMDLSAKTGTAETVAEGHPDITTVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHMNLTIAKEIMDAYYDMFMAK"
     gene            complement(2583..3431)
                     /locus_tag="IWM51_001572"
     CDS             complement(2583..3431)
                     /locus_tag="IWM51_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9970997.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3655..5595)
                     /gene="thrS"
                     /locus_tag="IWM51_001573"
     CDS             complement(3655..5595)
                     /gene="thrS"
                     /locus_tag="IWM51_001573"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP9970998.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5932..6111)
                     /locus_tag="IWM51_001574"
     CDS             complement(5932..6111)
                     /locus_tag="IWM51_001574"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP9970999.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6158..7981)
                     /gene="cadA"
                     /locus_tag="IWM51_001575"
     CDS             complement(6158..7981)
                     /gene="cadA"
                     /locus_tag="IWM51_001575"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9971000.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8259..10538
                     /locus_tag="IWM51_001576"
     CDS             8259..10538
                     /locus_tag="IWM51_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9971001.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10631..11278)
                     /locus_tag="IWM51_001577"
     CDS             complement(10631..11278)
                     /locus_tag="IWM51_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971002.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11457..12176)
                     /locus_tag="IWM51_001578"
     CDS             complement(11457..12176)
                     /locus_tag="IWM51_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9971003.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12307..13458)
                     /locus_tag="IWM51_001579"
     CDS             complement(12307..13458)
                     /locus_tag="IWM51_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP9971004.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13675..14415)
                     /locus_tag="IWM51_001580"
     CDS             complement(13675..14415)
                     /locus_tag="IWM51_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9971005.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14419..14742)
                     /gene="rsfS"
                     /locus_tag="IWM51_001581"
     CDS             complement(14419..14742)
                     /gene="rsfS"
                     /locus_tag="IWM51_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP9971006.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14799..15392)
                     /locus_tag="IWM51_001582"
     CDS             complement(14799..15392)
                     /locus_tag="IWM51_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9971007.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15376..16023)
                     /locus_tag="IWM51_001583"
     CDS             complement(15376..16023)
                     /locus_tag="IWM51_001583"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP9971008.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16036..16347)
                     /gene="yhbY"
                     /locus_tag="IWM51_001584"
     CDS             complement(16036..16347)
                     /gene="yhbY"
                     /locus_tag="IWM51_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP9971009.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16363..17472)
                     /gene="yqeH"
                     /locus_tag="IWM51_001585"
     CDS             complement(16363..17472)
                     /gene="yqeH"
                     /locus_tag="IWM51_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP9971010.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17469..17996)
                     /locus_tag="IWM51_001586"
     CDS             complement(17469..17996)
                     /locus_tag="IWM51_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP9971011.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18176..18961)
                     /locus_tag="IWM51_001587"
     CDS             complement(18176..18961)
                     /locus_tag="IWM51_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP9971012.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18954..19823)
                     /locus_tag="IWM51_001588"
     CDS             complement(18954..19823)
                     /locus_tag="IWM51_001588"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP9971013.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19824..21203)
                     /locus_tag="IWM51_001589"
     CDS             complement(19824..21203)
                     /locus_tag="IWM51_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP9971014.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21211..21630)
                     /gene="fabZ"
                     /locus_tag="IWM51_001590"
     CDS             complement(21211..21630)
                     /gene="fabZ"
                     /locus_tag="IWM51_001590"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9971015.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21655..22131)
                     /gene="accB"
                     /locus_tag="IWM51_001591"
     CDS             complement(21655..22131)
                     /gene="accB"
                     /locus_tag="IWM51_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP9971016.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22134..23369)
                     /gene="fabF"
                     /locus_tag="IWM51_001592"
     CDS             complement(22134..23369)
                     /gene="fabF"
                     /locus_tag="IWM51_001592"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP9971017.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23388..24125)
                     /gene="fabG"
                     /locus_tag="IWM51_001593"
     CDS             complement(23388..24125)
                     /gene="fabG"
                     /locus_tag="IWM51_001593"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP9971018.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24137..25054)
                     /gene="fabD"
                     /locus_tag="IWM51_001594"
     CDS             complement(24137..25054)
                     /gene="fabD"
                     /locus_tag="IWM51_001594"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9971019.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25161..25385)
                     /locus_tag="IWM51_001595"
     CDS             complement(25161..25385)
                     /locus_tag="IWM51_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9971020.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25438..26400)
                     /locus_tag="IWM51_001596"
     CDS             complement(25438..26400)
                     /locus_tag="IWM51_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP9971021.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26410..26850)
                     /locus_tag="IWM51_001597"
     CDS             complement(26410..26850)
                     /locus_tag="IWM51_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9971022.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         7360 a         5985 c         4688 g         8928 t
ORIGIN      
        1 cttttttgcc gagtaaacgg tgttcaaaag ccagtttctc ggtatgaagt caaatttaag
       61 caaaaatgtg agaagctatt ttcacaaaat ttttcttcat actcgaaact aagcggcttt
      121 ttcacgacct cttttttatt ttgccatgaa catatcataa tacgcgtcca ttatttcttt
      181 agcaatcgtc aagttcatgt ggtcttgttc gtctaataag ttcggcaaga caacactgat
      241 ggcaatttcc ggattgtctg ttggtccgta tgccacgata ttactattga ctgtcgtaat
      301 atctggatga ccttccgcaa ccgtttctgc agtccctgtt tttgcggaaa ggtccatttt
      361 cgcactggct aatggtttgg ctgctgtgta aggatcggta ccatggacaa cttggtggaa
      421 tccttcacgg aggatcgcca tattttctgc agaaatattg accgtatttt ctactgttgt
      481 gccaatttct ttcttcactt cacccaagtt tccattttcg tcattgccat agatcgcttt
      541 gactaggtgc ggctgtactc gtttcccgcc attagcgact gttgaagcat attgagctaa
      601 ttggattggt gtatatgtgt caaactgtcc aaatgccaaa tcggtaaagt tacctggagt
      661 ataccattct ttgccattat ccgcattgaa cttcttcata taatcaacag aacgtgagat
      721 cccaggagat tcattcggca aatcgatccc agtcgttgta ccaagaccaa actctttgaa
      781 agagtctctc aattgctgat aagctttagc ttcttcatct aaactaggta gtcccattcc
      841 aggtgtatag tctagtccta acatctttaa cgccactttg atcatatacg tattagaaga
      901 caattccaat gctttgactg catcaagagc aacttgtccg cttcgattaa agacagagct
      961 tttttctgaa gcaccttgca gacgaatcgg ctcatcaatc aaaacttgat ttccgctgat
     1021 cgcgttactt gcccagcctg ctgtcaaagt accagccttg actacagacc ctggagcaaa
     1081 ggcgctagta atcgtgccta aagcattctc ggtgatttct ttggaacctt gttcatgact
     1141 gaaaccagtc attgctagca cttctcctgt ctgaggattc atagccactg cataagcacc
     1201 aggcgaatac tgtgctttgc cgttgttgat caatgtttga taattccttt tcaaaatttc
     1261 ttctactttt gattggaatt ctgcattcat agataacatt aaattagaac ctttttcccc
     1321 agagaagatt tctttttgat tcgaaacatt tccttcatta tcaagggaaa cttcatattg
     1381 ggttttggtt ccttgcagga catcttcata ttgtttttcc aaataacttt gtcctacacg
     1441 gtcattccga gaataacctt ttgctaggta tttctctgct tcatctgctg gaagtccttg
     1501 cttttcagtc gtcacactgc caagaatact tttcagtgaa cttgctgcat tatattctcg
     1561 tgtccagtct gtgccagtcg aaactcctgg aagttcagaa gcgcgttctg ccacaacagc
     1621 taactcttga tcagtaacac cagagttttt gatatagaca gtgtttaatt catatgcact
     1681 attcatccgt ttaaagatgg tagcaatttt taattgctcc tcgtttaaat taatctcatc
     1741 agctgtgact ttattgacca tcgcactata ttgttcgctg ttccctaatt gcttttcttt
     1801 tgccgaaagt ctttttgtgg cttctttcaa atgtttggga tcagccagcc aaaaatcttt
     1861 tttatctcta tctgtcagat tttcatctac cggaacatcg atcagcgagt tcagcttcgt
     1921 tgcgatttca agcaaatctt tagccgtaac ttgtgttcct ctcgtatatg tgatggcctg
     1981 attcgcttta ttcgtaacta aagcttttcc tgtggagtcg tagatcattc cgcgaggagt
     2041 actttcttgg accgtaatcg tagaagtaga cttcactcgc gcctccatct cttcgccgtt
     2101 cacgatctgc aaataaccca atctagcaat caacgcaaca aataacgtaa atataatgaa
     2161 aaaaagaaaa tttaagcgaa aaggaacatg ggaccggcga aacgttttgt tcttaggttc
     2221 ttgtgcacct tttcttattt tttctaacca agaagtattt aatatttttt tcataaaatc
     2281 tttcatcatt tcctatcctt ccgcctattt acattcatgc ttcattgtac ccaaaaactt
     2341 cttaataaaa aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa
     2401 tcaacttttt gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa
     2461 aaaataaact tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca
     2521 tttatttgtg gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt
     2581 ttttatttcg aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg
     2641 ctaagctttt cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg
     2701 gccaaaagtg aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt
     2761 acgagtacat cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt
     2821 tcgatattca tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag
     2881 tttgaaacaa gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg
     2941 tggatatctt tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct
     3001 tctactacta aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa
     3061 cactctacga ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct
     3121 acttgtgcat tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata
     3181 tcactaaaac cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc
     3241 gtcagacttt gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg
     3301 gaagcttctg ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg
     3361 tagttggcca actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta
     3421 attacgctca taagttcctc caccctttta atgatagtct atgtaagtat accatatcag
     3481 acccttattc acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata
     3541 tcttatccca tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt
     3601 atctattgtt cggattgctt tctatctact acttttttac aggaaattta cctattagtt
     3661 ttctctgctg taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc
     3721 tacagctgtt tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt
     3781 gtctccaaca actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc
     3841 cattttttca ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt
     3901 gatttcataa gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat
     3961 aggtgccaac catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg
     4021 ttccattgta gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc
     4081 ttctccaaca tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga
     4141 taatgtttct tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa
     4201 ggctgcctcg ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag
     4261 cattgcttgg gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg
     4321 atcgcgatag ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac
     4381 catcaattca agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg
     4441 accatcattc aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc
     4501 atagcgatgc atcattccta attcagcgat acgaattggt aattcacgat aagaatggat
     4561 cgtgtttttg tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc
     4621 gccatctccc atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga
     4681 tgttttgtac aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact
     4741 aacttctttg tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag
     4801 ccagaatggc aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc
     4861 tagtttacga tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc
     4921 tttcttgtca aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc
     4981 acgccaataa gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg
     5041 aacatgtggt ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc
     5101 ttcttctggc aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa
     5161 tgcttcctgt tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat
     5221 catttctgct tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt
     5281 atcgtagtaa aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag
     5341 gcgacgcatg gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc
     5401 ttcatggtct ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc
     5461 aacgagtttt ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct
     5521 ttcagcaatt gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa
     5581 agtaattttt atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc
     5641 tgaaaaacaa aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg
     5701 ttccacctaa tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct
     5761 tgcttcaaca agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc
     5821 ctagcttttc ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt
     5881 atgtgtttat ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt
     5941 ccattcccca tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg
     6001 ttcttttggt gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc
     6061 taattgttcc ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc
     6121 tcctctttct gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta
     6181 ctgaatcagg aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg
     6241 tatccacgac ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa
     6301 ttaacatcaa aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca
     6361 tcgtatctct tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga
     6421 ccgcatctgc actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat
     6481 ctgcagcagc aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt
     6541 gatctttagg gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta
     6601 cagaatctaa ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga
     6661 tcatcatcaa atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg
     6721 ggcgaatctg atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga
     6781 ttgctgtatt ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta
     6841 cttcatgatt attgactact gcttggattc cttgaccggt cacttctttc agttgactaa
     6901 caggatacag ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag
     6961 attcctgttc tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg
     7021 gctgaatcgc ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa
     7081 tcgtttttgt acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt
     7141 tacttgaacg gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag
     7201 gtgatgccac taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa
     7261 taccaccgat caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga
     7321 caaatcgggc aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc
     7381 tgtctgcggc acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg
     7441 acataatctg atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt
     7501 catcgaccga agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct
     7561 tgactagtaa ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt
     7621 ctttgaatcg atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg
     7681 ctcgtttatt cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca
     7741 aactagccca gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat
     7801 ctacgccata tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga
     7861 gaatccctcc agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt
     7921 cacttaataa agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca
     7981 ttgttttttc tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc
     8041 tttcatctta agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa
     8101 caactatcac tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa
     8161 ggattacttt ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact
     8221 tttaattcgt tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac
     8281 aaacgcacgt ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa
     8341 aacaacaaga attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga
     8401 ttcgagctgg ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc
     8461 aggaattgct tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt
     8521 ctgtcatgaa agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag
     8581 aaacattata tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg
     8641 actggcgcgc accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg
     8701 aaactgaaac agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag
     8761 aaggacgtat tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag
     8821 agatattgga tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag
     8881 cacaaaatgc aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg
     8941 gaagtggaaa gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca
     9001 agtggctcga ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca
     9061 tctcgactgt attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt
     9121 atttggtgca gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt
     9181 ttttgctagg ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc
     9241 agattgatcg ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa
     9301 taaatggccg tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg
     9361 aacaattacc tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag
     9421 gcggtctgca aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc
     9481 tccaagaact atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat
     9541 tacgtaaaaa actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga
     9601 tcatgaatta tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc
     9661 cactttctct tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa
     9721 ctgttcgtac acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt
     9781 tactgatgcg ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg
     9841 atgaaatgca ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag
     9901 caaatttgac tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg
     9961 gatcattgga agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct
    10021 atcgctccac aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag
    10081 tagaaacaac agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata
    10141 tcaaattgaa ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc
    10201 gtacagcaat catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag
    10261 acgagttgaa acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca
    10321 ttattttact tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg
    10381 atgtagatgc agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta
    10441 cacgtgcgat gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca
    10501 atatggatat ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat
    10561 taatgttagc agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat
    10621 ttaaggtcat ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa
    10681 gcgaaataat cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc
    10741 tatgacggcc aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt
    10801 ggtcaattga ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac
    10861 gaaatgctag gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc
    10921 tttcgataag aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag
    10981 cctccatccg atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca
    11041 gcggaatcag ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta
    11101 aataacggtt cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga
    11161 atcttgcgta tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta
    11221 ctttgcggta accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat
    11281 tcttccccgt ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc
    11341 ataccacagg ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta
    11401 gtgagattgt cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag
    11461 tcttctaatg ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca
    11521 atcatttttt caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc
    11581 atttggatct cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata
    11641 ctatggaaat ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg
    11701 cgaacatcga tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca
    11761 aaagcaaaga ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc
    11821 ccgccatttt tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc
    11881 aatgtatcaa caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag
    11941 tatgtttcat ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt
    12001 acattataag ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca
    12061 ggatcacctg attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta
    12121 ttattagcat ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt
    12181 tcacttcgct ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt
    12241 gtgaatcacg attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc
    12301 aattatctat actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa
    12361 ttgatagatt tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat
    12421 tcgggaataa atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa
    12481 tccaagtagt cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt
    12541 cagcaagaca taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac
    12601 ctttgacaca aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc
    12661 tattccttcg gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg
    12721 ataacgcaaa tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc
    12781 ttctgtctga ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat
    12841 ggctgttgca ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt
    12901 acgtcggatc ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact
    12961 taatcccaag atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg
    13021 aaaaacctca gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc
    13081 ctcttctatc ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga
    13141 agtctcttct tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac
    13201 tgcaccttta gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac
    13261 taaatccaca ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg
    13321 ttcgcctctt tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat
    13381 ttttcgtgcc atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat
    13441 aatcccgcag cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga
    13501 caagtaaaaa aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa
    13561 cacaatactt ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat
    13621 ataatttatg ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc
    13681 tttgtatgct acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc
    13741 agaaaatgct tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa
    13801 tggataggta cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg
    13861 ttcttcaacg aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact
    13921 atcccacaaa aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc
    13981 atctacttga taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg
    14041 atagacttca tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc
    14101 agaaaaacag gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc
    14161 cacaaattgg atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc
    14221 ttctgaaagg tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc
    14281 tccagtaccg catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga
    14341 aaaagctaac cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa
    14401 tgtttcataa gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga
    14461 ccatagtttt tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac
    14521 atctccaaga tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc
    14581 atatttgttt tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc
    14641 gctatttgct gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa
    14701 agcgataatg tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc
    14761 tttactatct atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa
    14821 ttatatgttt cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat
    14881 gtatgttttg tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca
    14941 tcttgtaccc caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg
    15001 tcaaggagac tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct
    15061 gcatcggtga ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt
    15121 gcattattat aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac
    15181 tcctcatctg gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca
    15241 ggagaagcac cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc
    15301 ttgaagcgtt gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa
    15361 gcagtatatt cattactata gtccatattc atacaaccct tcattttgaa tataatcaat
    15421 tactttatct ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt
    15481 cgtggaactg atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt
    15541 tgtgtatcct ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt
    15601 ataccatttt ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc
    15661 cggattgttt tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc
    15721 taattcaatc gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt
    15781 gagtcgatgt tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg
    15841 catcaaaaat acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg
    15901 tgcaaggtga acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa
    15961 gaattgaggc atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt
    16021 catcatggtc actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt
    16081 tgtggaaggt ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat
    16141 ttctttttct aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga
    16201 tactttgatc agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt
    16261 gatcccacct tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa
    16321 acgcttttgt tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg
    16381 tttcaataca tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt
    16441 gatccagcct agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg
    16501 aacaagctcc ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac
    16561 atgtttttga taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt
    16621 tgacacataa gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc
    16681 gcccaagaaa agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa
    16741 cggtgcaatg atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat
    16801 aattcccggt gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa
    16861 ggtcgttcct ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat
    16921 gatttgattg ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg
    16981 tccatcgcgg tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc
    17041 actagttaat aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca
    17101 ttgtgttaat ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa
    17161 aagtactgga ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc
    17221 aaaaatatcc accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg
    17281 caaaaagtcg tcatccgtca atgagacatc ttggatctca ttataatggc gtaatctgaa
    17341 acaacgctga caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt
    17401 gtatcctaat tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa
    17461 ttcttctgtc attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag
    17521 gccataattc ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata
    17581 atgggtttta caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata
    17641 atctgatcgc ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg
    17701 ttgatccctc ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca
    17761 atcgctcgcg cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca
    17821 tttttcattt ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca
    17881 atcaacgtat tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca
    17941 ggtgtaatct gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg
    18001 tctcctcatc tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact
    18061 gcatatcagc aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt
    18121 acaaaatgac caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat
    18181 attggcggaa cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt
    18241 ccgtcaatct ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact
    18301 gctcgccgtt gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg
    18361 gcgcagtaat tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa
    18421 tcgacgcaaa tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt
    18481 catccgctaa acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga
    18541 tgatcggtac ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc
    18601 cgcgctcttc tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg
    18661 gtctattgaa cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat
    18721 ggggagaacc aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga
    18781 tcactgttac cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg
    18841 caaataaacg atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca
    18901 agcttgtcca tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca
    18961 cgatctatcc atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat
    19021 tccatgcggg gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg
    19081 aaatcttctg gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg
    19141 atcaagcttt ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact
    19201 cctccggtcg tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt
    19261 ttgacagcag cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt
    19321 gcgccacctg aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat
    19381 aatctggtga tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc
    19441 atcactccaa taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca
    19501 tgaagtcctg ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc
    19561 ggatctttgg ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa
    19621 gctagacgct gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct
    19681 tcaccgatgt ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta
    19741 tcaggaacag atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat
    19801 ttctttttct taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc
    19861 ggtaaaaatt cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga
    19921 ctaatcaaat ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt
    19981 aaggctcgct gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt
    20041 gcaatcattg aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact
    20101 cgcagtccca tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca
    20161 aaatggaaag caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact
    20221 acttcttctt gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact
    20281 aaatcgattc ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt
    20341 tccatgaaat aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca
    20401 taattgactg ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt
    20461 gagatcacta cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa
    20521 tctcgctcgc ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata
    20581 tggcgagcag gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct
    20641 gcttcttgct gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga
    20701 atgcctttcc cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct
    20761 gctatttcca atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg
    20821 acatttgctt cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt
    20881 gcacttttag gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta
    20941 ttttcagata aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg
    21001 ctcagtactt cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt
    21061 gcttcatctg ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg
    21121 gtttggatcc ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta
    21181 gctatcaata ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc
    21241 ggctactttt ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac
    21301 tttcaatatc tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt
    21361 tgctttgtcc aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa
    21421 tgcaactgct cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc
    21481 ttctggaaaa tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat
    21541 ccgttctcct tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg
    21601 aatgatttct tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt
    21661 ttgctgaatc ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat
    21721 ttctgtgatc actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat
    21781 aatgcagata acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc
    21841 gggcgctggt tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa
    21901 tactgccgat tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac
    21961 attgactggc tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact
    22021 tgttttattt ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag
    22081 cgaggattga tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc
    22141 tcccagcgtt tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt
    22201 gcataagtaa aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga
    22261 tcttgttttt ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt
    22321 gccaaggctt cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg
    22381 ctgacgtaag tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc
    22441 tggtcattgg ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt
    22501 acaccagctt cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta
    22561 ggagctgtca tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt
    22621 ttggcaccgc gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca
    22681 cccatcacaa aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt
    22741 gattctgaga gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct
    22801 tctgttcctc cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc
    22861 ccaattgaat tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca
    22921 cctacacgca aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt
    22981 ggagaaactc gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga
    23041 agaccaccga tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta
    23101 tctaaaccgc tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata
    23161 tccattcgtt tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca
    23221 cccgctaagg taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc
    23281 gtcccctctt ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact
    23341 ccatagccag taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg
    23401 accaatccgc catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa
    23461 actgctgttt gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc
    23521 gcttgttcct tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc
    23581 gcaatggcat tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat
    23641 cctactacgc cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca
    23701 gatacactag agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg
    23761 tgctgcgtca tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct
    23821 gcatccattc gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg
    23881 atcgatccaa gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag
    23941 atgtcaccgg aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct
    24001 tttggaatct ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag
    24061 gccatcgctt tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca
    24121 tccataacgt tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa
    24181 cacgcagaac tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg
    24241 ggccgacttc gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga
    24301 aacgaacagg ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta
    24361 tttccgttgt tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta
    24421 ggtccttcgc cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga
    24481 catttagagg gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta
    24541 ctgctttttc ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata
    24601 cgatgccgta cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca
    24661 tcactgcaac cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt
    24721 ttgctactaa cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat
    24781 attctcctaa actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc
    24841 gataatacgc gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac
    24901 gttcattttc tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat
    24961 caaatgtctg ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg
    25021 caccttggcc actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat
    25081 atgtttgatt cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc
    25141 ccccaaccta attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga
    25201 accgttttga ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg
    25261 atgatttgga ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat
    25321 ttgatttctt cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat
    25381 accataataa tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta
    25441 caactccatg agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt
    25501 ctgctgtttt cctaaggtga tctttccact agaaactgct tcatccaata agatagggat
    25561 gcttgcagca gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat
    25621 ctttactttt cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata
    25681 atcaactggt ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa
    25741 atcaaaaata tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata
    25801 gaacggattt ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc
    25861 tgctaatttt tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc
    25921 tccatcgcca aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt
    25981 ttcaccacca atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct
    26041 taacgcataa acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc
    26101 tcccaatttc ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt
    26161 tgctactaaa ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc
    26221 cgccactaaa atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat
    26281 acccgtcctt gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt
    26341 agtaataatc ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat
    26401 taaatatcct cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa
    26461 ggcatgctgt tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa
    26521 atgctgatgg acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg
    26581 gcggtcatct tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc
    26641 agtggtcaag gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt
    26701 cttgtacatc ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg
    26761 agattttttc agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata
    26821 atcatttact gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat
    26881 caaatctttt gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc
    26941 atttttttac tatatgagct t
//
LOCUS       DACDYJ010000037        26529 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDYJ010000037 DACDYJ010000000
VERSION     DACDYJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.037"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWM51_001598"
     CDS             complement(99..1229)
                     /locus_tag="IWM51_001598"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP9971023.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IWM51_001599"
     CDS             complement(1878..3080)
                     /locus_tag="IWM51_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9971024.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IWM51_001600"
     CDS             complement(3140..3343)
                     /locus_tag="IWM51_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9971025.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IWM51_001601"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IWM51_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IWM51_001602"
     CDS             4880..5692
                     /locus_tag="IWM51_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9971026.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6868)
                     /locus_tag="IWM51_001603"
     CDS             complement(6716..6868)
                     /locus_tag="IWM51_001603"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971027.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="IWM51_001604"
     CDS             complement(7059..7937)
                     /locus_tag="IWM51_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9971028.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="IWM51_001605"
     CDS             complement(7976..9094)
                     /locus_tag="IWM51_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9971029.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="IWM51_001606"
     CDS             complement(9091..11190)
                     /locus_tag="IWM51_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP9971030.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="IWM51_001607"
     CDS             complement(11241..12062)
                     /locus_tag="IWM51_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9971031.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="IWM51_001608"
     CDS             complement(12059..12868)
                     /locus_tag="IWM51_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9971032.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="IWM51_001609"
     CDS             complement(12889..13383)
                     /locus_tag="IWM51_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9971033.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="IWM51_001610"
     CDS             complement(13395..13883)
                     /locus_tag="IWM51_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9971034.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="IWM51_001611"
     CDS             complement(13960..16656)
                     /locus_tag="IWM51_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9971035.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGDTGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="IWM51_001612"
     CDS             complement(17084..18157)
                     /locus_tag="IWM51_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9971036.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="IWM51_001613"
     CDS             complement(18154..18981)
                     /locus_tag="IWM51_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9971037.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="IWM51_001614"
     CDS             complement(18982..19788)
                     /locus_tag="IWM51_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9971038.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="IWM51_001615"
     CDS             complement(19800..20885)
                     /locus_tag="IWM51_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9971039.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="IWM51_001616"
     CDS             complement(20902..21444)
                     /locus_tag="IWM51_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9971040.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="IWM51_001617"
     CDS             21702..22691
                     /locus_tag="IWM51_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9971041.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="IWM51_001618"
     CDS             22688..23425
                     /locus_tag="IWM51_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP9971042.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="IWM51_001619"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="IWM51_001619"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP9971043.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNTITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="IWM51_001620"
     CDS             23989..24621
                     /locus_tag="IWM51_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9971044.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="IWM51_001621"
     CDS             24706..25353
                     /locus_tag="IWM51_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9971045.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="IWM51_001622"
     CDS             25358..26341
                     /locus_tag="IWM51_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP9971046.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8115 a         5600 c         4498 g         8316 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt gtctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttgccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaatg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaacac gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgct
//
LOCUS       DACDYJ010000038        25361 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDYJ010000038 DACDYJ010000000
VERSION     DACDYJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.038"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(135..512)
                     /locus_tag="IWM51_001623"
     CDS             complement(135..512)
                     /locus_tag="IWM51_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971047.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            768..1496
                     /locus_tag="IWM51_001624"
     CDS             768..1496
                     /locus_tag="IWM51_001624"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP9971048.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1496..1750
                     /gene="purS"
                     /locus_tag="IWM51_001625"
     CDS             1496..1750
                     /gene="purS"
                     /locus_tag="IWM51_001625"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP9971049.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1752..2423
                     /gene="purQ"
                     /locus_tag="IWM51_001626"
     CDS             1752..2423
                     /gene="purQ"
                     /locus_tag="IWM51_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP9971050.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2424..4646
                     /gene="purL"
                     /locus_tag="IWM51_001627"
     CDS             2424..4646
                     /gene="purL"
                     /locus_tag="IWM51_001627"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP9971051.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4631..6070
                     /locus_tag="IWM51_001628"
     CDS             4631..6070
                     /locus_tag="IWM51_001628"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP9971052.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6102..7145
                     /locus_tag="IWM51_001629"
     CDS             6102..7145
                     /locus_tag="IWM51_001629"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP9971053.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7142..7720
                     /gene="purN"
                     /locus_tag="IWM51_001630"
     CDS             7142..7720
                     /gene="purN"
                     /locus_tag="IWM51_001630"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP9971054.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7717..9252
                     /gene="purH"
                     /locus_tag="IWM51_001631"
     CDS             7717..9252
                     /gene="purH"
                     /locus_tag="IWM51_001631"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP9971055.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9268..10542
                     /gene="purD"
                     /locus_tag="IWM51_001632"
     CDS             9268..10542
                     /gene="purD"
                     /locus_tag="IWM51_001632"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP9971056.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10664..11578
                     /locus_tag="IWM51_001633"
     CDS             10664..11578
                     /locus_tag="IWM51_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP9971057.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11711..13276)
                     /locus_tag="IWM51_001634"
     CDS             complement(11711..13276)
                     /locus_tag="IWM51_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9971058.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13269..14720)
                     /locus_tag="IWM51_001635"
     CDS             complement(13269..14720)
                     /locus_tag="IWM51_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9971059.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14734..15750)
                     /locus_tag="IWM51_001636"
     CDS             complement(14734..15750)
                     /locus_tag="IWM51_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9971060.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15970..17058
                     /locus_tag="IWM51_001637"
     CDS             15970..17058
                     /locus_tag="IWM51_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9971061.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17112..18638
                     /locus_tag="IWM51_001638"
     CDS             17112..18638
                     /locus_tag="IWM51_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP9971062.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18640..19845
                     /locus_tag="IWM51_001639"
     CDS             18640..19845
                     /locus_tag="IWM51_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9971063.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20102..21079
                     /gene="guaC"
                     /locus_tag="IWM51_001640"
     CDS             20102..21079
                     /gene="guaC"
                     /locus_tag="IWM51_001640"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP9971064.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21343..22767
                     /locus_tag="IWM51_001641"
     CDS             21343..22767
                     /locus_tag="IWM51_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9971065.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23228..24337
                     /gene="pdhA"
                     /locus_tag="IWM51_001642"
     CDS             23228..24337
                     /gene="pdhA"
                     /locus_tag="IWM51_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP9971066.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24340..25317
                     /locus_tag="IWM51_001643"
     CDS             24340..25317
                     /locus_tag="IWM51_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP9971067.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8204 a         4317 c         5696 g         7144 t
ORIGIN      
        1 aaccaaaaaa gaagcagatc cgttagcggt tctgcttctt ttagtttatt atattttctt
       61 atcaataata ggaacctgat tttaaaagcg gctacctctt taccgtgcct ctttacatac
      121 aaaaaataga ggaattaagc gtaaatggct tggctattat aactaaaatg ctggttatgg
      181 agatgttgca gcaagttttc gagaaaagcg tattccatct cttgtccatt gaataagatc
      241 tggatacgat aatgattttt gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg
      301 cctttgtgtt ctgtctgata aagaaaagtc cttttacggc attcagcaca gcgcttggtt
      361 gctcggcgga tttttcggaa tgtataaaaa ctagtatctt tattgcataa ataaacgtct
      421 ttgatcgaca gcaaatcatt atatagacag gaacgttctt cagctgttaa ttctgataaa
      481 aaaagctgac cttgttttat tgcatcttcc atcggcaagt gcctcctcga acccattatc
      541 caaattttta gttaattcta atataccatc attaagtata aatgtaaacg cttgttgatg
      601 gtataagatt ttcgtttctg ataaaacacg aacattaaaa gaatctaatg ccttaaatgt
      661 acatattttt gttgatttat aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca
      721 aaaaacgatg tatttgcttt taatgtaaat aaaaaggaga gacgtacatg gagaagaaag
      781 aacttttgta tgaaggaaaa gccaaaaaaa tatatcttac ttctgatgag aacttgattt
      841 ggatagaata tttagatcaa gcaactgcgt tgaatggcgc gaggaaagac cagataagtg
      901 gaaaaggaga attgaataat caaatcacaa gtgcaatttt ccgttatctg actcaaagag
      961 gaataaaaaa tcattttatt gaacaattgt ctaaaaccga acaattagca aaaaaagtac
     1021 aaattatccc tctggaagtg gttatccgaa atattgcagc aggaagtttc tcaaaacgtc
     1081 taggtgttcc cgaaggtaaa gaattcatca aaccaatcat tgaattctac tataaggatg
     1141 accagttgga tgatccattt atcaatgaag agcatatcta tttcttacaa cttgcaaagc
     1201 cggaagaaat cagtgagctg aaagcccaag cgttggctat taatgaaatt ttgaccgaat
     1261 tatttgctaa ggtcggtttg gttttagtag atttcaagct agaatttggc cggtcgaatg
     1321 gacaaatttt gcttgcggat gaagtaacac cggatacttg ccgattatgg gatgaacgta
     1381 cgaatgaaca tatggacaaa gacgtttacc gcagagatct aggtgatttg atcccagtct
     1441 atcaagaagt atataaccgt ctatcggaac taactgaaga aaaggagcga gtatgatgta
     1501 tttcgtaaaa gtatatgtaa cctataaaga ttcagtgtta gatcctcaag gggaggcagt
     1561 aaaaaaagca gtccatcgta tgggatatga aacaatcgaa gatatccgaa taggaaaata
     1621 ctttgagata caacttgctg atgcagaaga accaatcgat acagtgattg aaacgatttg
     1681 tgacaagctg cttgcaaatg taaatatgga aacataccga tatgaaatca aaaaaatgga
     1741 ggaagtataa catgaaattt gcggtcatcg tttttccagg ttcaaattgt gacgcagata
     1801 tgttatgggc agttcgcgag atcatgggcg ctgatgccga atttgtacgg catgatgagt
     1861 cctctttaga aggatttgat ggggttttac tgccgggagg attctcctat ggtgactatt
     1921 tgagatgcgg tgcgattgct cggttttcta caatcatgga agaagtgatt cgttttgctg
     1981 aagaagggaa accagtattc ggtacatgca atggattcca gatattgaca gaagcaggac
     2041 ttttgccagg tgtactgaga cgaaatgagt ctttgcattt tatttgcaaa actgtaccat
     2101 tgaaagtcaa ccccaagacg gcctttactt cagagtatga agccgacgaa ataatctatc
     2161 ttccagttgc tcatggggaa ggaaattatt tctgtgacga agaaacactt caagaactaa
     2221 aaaaaaataa tcagattgcc tttacttata atgacaacat caacggcagc gtggaaaaca
     2281 ttgcagggat tacgaacaaa gcagggaatg tgttaggaat gatgcctcat ccagaacgtg
     2341 cagtagaaga cttgctagga tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa
     2401 acttcggaaa ggtgaccgta tagatgaaaa cattgaaaga accgacagct gaagagatca
     2461 agaaaaaaag aatctatgcc gaatggggat tgactgatga agaatatcga atgatcgaag
     2521 aagatatctt aggaagaatg cctaattata cggaaactgg actgttttct gttatgtgga
     2581 gcgaacattg ttcctataaa aattctaaac cggttctacg caaatttcct actagtggac
     2641 ctcatgtgtt gcaaggccct ggagaaggcg caggcatcgt cgacatcggt gatggactag
     2701 cagttgtctt caaagcagaa agtcacaatc atccttcagc cgtggagcct tatgaaggag
     2761 ctgcgacagg agttggcgga atcatcagag atattttcag catgggggct cgtccaattg
     2821 cgttgctaga ttcattgagg ttcggcgaat tagataatcc aagaacgaaa tatttactag
     2881 aagaagtagt tgcaggtatc ggcggatacg gcaattgtat cggcataccg actgtaggcg
     2941 gcgaaattgc atttgatcct tgttatgaag gaaacccatt agtcaatgca atgtgcgtag
     3001 gtttgattga acacaaacat atccagaaag ggcaagccgc tggcgtcggt aattcgatca
     3061 tgtatgtagg tgcaaaaaca ggtcgcgacg gtatccacgg agcaactttt gcctcagaag
     3121 aatttaatca agaagaagaa cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg
     3181 aaaagctgct gcttgaagcg tgtttggaat taatcttgca tcattcggat tctctaatag
     3241 gtatacagga tatgggagca gcaggacttg tttcatctag tgcggaaatg gcttcaaaag
     3301 ctggctcagg acttatattg acattagatg aagtcccaca aagagaaact gggatgacac
     3361 cttatgaaat gatgttatcg gaatcacaag aacggatgtt gatttgtgtt aaatcaggag
     3421 aggaagaaaa aatccaagaa ctatttcaga agtatgatct agatgcagtc actatcggta
     3481 aagtaacaga cgacgggcaa tatcggctat atcatcaagg agaagaagta gcaaatctgc
     3541 cagtcgatgc attagctgaa gatgcaccag tttatcataa agaaatgaaa gaaccagctc
     3601 gaatcgtaga atttcagcag ttagctcctt atcagccagt tatcgaagaa cctggacaag
     3661 tattgcttga tctgctgcag caaccgacga ttgcttcaaa acgttcaatc tacgaaactt
     3721 atgattcaca agtacaaacc aatacagttg tccgtccagg aagcgatgct gctgtgatga
     3781 gggttagagg gacgaataaa gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt
     3841 atttggatcc aaaaatcgga ggacaaatag cagtggcaga agctgctaga aatattattg
     3901 ctagcggtgg taagcccttg gcgatcaccg actgcctgaa ctatggttct cctgataaac
     3961 cagaagtttt ttgggaatta tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt
     4021 taggaactcc tgttatttca ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa
     4081 tctatccaac accaatgatt ggtatggtcg gattgattga ggatcataaa catatcacta
     4141 ctcaggaatt caaaaaatca ggggatctaa tctatatatt aggtaagacg tttgcagatt
     4201 ttgatggcag tgaattacaa aagatgcagc ttggaagaat cgaaggtgtg atccgaaact
     4261 ttgatctatc cattgaaaag agaaaccaag agttagtatt gacagccatc caaaacggat
     4321 tgattgaaag tgctcatgac tgttcagaag gtggcttggc aattgcactg gcagaatcag
     4381 ctttcaaaca tcagctaggg atatctgtac agtttgaatt gtcctctgct caattatttg
     4441 cagaaactca atcaagattt gtgttgacag tcgcaccgga aaataagaca cgattcgaag
     4501 aaatgatggg agatgctgct gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa
     4561 tttcagcaac tgacggtcag atcaaaatag aaactgcagt agcaagaaag tgctgggagg
     4621 atgcaatcgt atgtcttatg aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt
     4681 ttggggtcat ccagacgccg ctagtgtggc atacttcggg ctccatagtc ttcagcatcg
     4741 aggacaagaa ggtgcaggta tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga
     4801 tttggggctg ctttctgagg tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga
     4861 agctgcaatc ggacatgtac gttatgcaac agcaggcaat ggaagtgtgg acaatattca
     4921 gccttttcta ttcaaatttc atgatcagtc gatcggctta gcgcataatg gtaacttgat
     4981 caatgctcgt tcccttagaa aagagttaga ggaaaatggg gcgatcttcc attctaactc
     5041 cgatacggag atattgatgc atttgatccg acgaagtgaa gaaacagatt ttttaaataa
     5101 attaaaaagt gctttgaatg aagtcaaagg tggctttgcc tatttattaa tgacagaaac
     5161 ggcaatgatt gccgctttgg acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa
     5221 aaacggagca tacgtgattg cctcggaaac ttgtgcatta gaagttattg gggcacgttt
     5281 tataagagat gtccagcctg gtgagatagt gatcatcaat gacgaaggaa tccagattga
     5341 ttgctttacg aaggacacgc agctttctat ttgttcgatg gaatatatct attttgccag
     5401 accagattca aacatcgcag ggatcaatgt gcatactgca cggaaaaata tgggacggaa
     5461 tttagcgaaa gaatcgccag taaaagcaga tatggtcgtt ggcgtaccta attcttcttt
     5521 gtcagccgca agcggttatg cggaagctag tggaatccct tatgaaatcg gattagtaaa
     5581 aaatcaatac attgcccgaa catttatcca gccaacgcct gagctaagag agcaaggtgt
     5641 tcgaatgaaa ttatctgctg tacgaggagt agtggaaggt aaaaaggtga ttatggtcga
     5701 tgattcaatt gttcggggta cgacaagcag acggatcgtc catcttttga aagaagcagg
     5761 ggcaaaagaa gtccatgtga ggattgcttc tcctcctttg aaatatccat gtttttacgg
     5821 gattgatatt caaacgcgta aagaactcat tgcggcaaat cacacgattg atgaaatcaa
     5881 agaatgtatc ggtgcagatt ctttaggttt tttaagtgaa gagggtctga tcgaatcgat
     5941 cggtctggaa aaagatgctc cgtattcagg attgtgtatg gcttatttca acggagatta
     6001 tccgacgccg ttatatgact atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc
     6061 gttcttttaa aagcagacca agaagggagt atatgaattc aatggcaaat gcatacgcaa
     6121 aagctggcgt agatgtcgaa gctggatatg aagtagtaga acggatcaag aagcatgtca
     6181 aaaaaacgga acgtttaggt atgatgggcg ctataggagg attcggcggt tgttttgatt
     6241 tatcggtctt tcaattaaaa gagccagtcc ttgtctcggg gaccgatggc gtggggacga
     6301 aattagcagt ggcaatcgaa gcagacaaac acgatacaat cgggatcgac tgcgtagcga
     6361 tgtgtgtcaa tgatatctta gcacaaggcg cagagccgtt atatttctta gactacttag
     6421 caacggggaa aaatgatccg ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat
     6481 gtgttcaagg aaatatggcg ctgattggcg gagaaacagc tgaaatgcct ggtatgtact
     6541 caggtaatga ttatgatcta gccggatttg ctgtagggat agctgaaaaa tctcaattat
     6601 tgacaggaga aacgatcaac ccaggagatg tgttgcttgg aatagcatct agcgggattc
     6661 attcaaatgg gtactctctt gttcgcaaaa ttttctttga gcagcagcag ttttctttat
     6721 catcaaaact gccagaattg aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa
     6781 tctatgtttc ttccttgctt ccgctgatca aagaaaagaa aataaaagga gctgcacata
     6841 tcactggtgg agggtttgta gaaaatatcc cgcgaatgtt accagaatct atggctgcac
     6901 aaatcaaact tggctcatgg ccgattcttc caatattcgg ctgtttacaa aaatatggtg
     6961 aaattcctga gatggagatg tatgaaatct tcaatatggg catcggtatg gtcctggcag
     7021 tcgaagaatc gaaagtaaaa gaaatagtac acgaaattga gcaagctggc gaaaaagttt
     7081 atgaaatcgg gaaagtgatc gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac
     7141 gatgagaatc gctgtttttg cttctggaaa cggaagcaac tttcaagcgc tagccgatta
     7201 tctatcgaaa aaaggattgg aatcttcgat tgactggttg ttttgtgatc agcctgaagc
     7261 atatgttttg aaacgtgcaa ctgcgttgag tgtaccggca gattgttttt cacctaaaga
     7321 atttgattca aaaaaagagt atgaagaagc aattttgcat aaattgaaag aaaaaaagat
     7381 cgatttgatc gtattagctg gatatatgcg aatcattgga ccggtattgc tgaaaaatta
     7441 tgataaaaga atcatcaata tccatccgtc tcttttacct gccttccctg gtttgcatgg
     7501 catacgtgat gcctttgaag ctggagtaaa agaaacgggt gtcacaattc attatatcga
     7561 ccaaggtgta gatactggac caatcatccg gcaggaaaaa gtgagaattg aacaggagga
     7621 cacgttcgat tctttagaag aaaaaatcca tagagtagag catcgaatct atccagaagt
     7681 gattagtgaa attattgaaa atggaggcta tctgaaatga caagagcatt gatcagtgta
     7741 tcagataaaa ctgggattgt agcattcgcc caaggtttga ttgaacaagg agtagaaatc
     7801 atttcaacag gaggaacaaa gcatgcgttg gataaagctg ggatcaaaac gctttcgatc
     7861 gaagaagtga cagggtttcc tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa
     7921 atccatggtg gtcttttagg aagacgcgat ctccctagcc atatggaggc aatggaaacg
     7981 gaaaatatcc aaccaatcga ctttgtttgt gtcaatttat atcctttcaa agaaacgatt
     8041 atgaaaccag atgtcgaaat agcagaagct attgaaaaca ttgatatcgg tggtcctagt
     8101 atgctccgta gtgcagcaaa aaaccatgaa tttgtcacct ctgttgttga tccatctgac
     8161 tatgaagaag tactttcaga attacgtcag tatgggcata cttcactttc tttcagacaa
     8221 aaactagcgg caaaagtctt tcgtcatact gcggcatatg atgcactgat cgctcaatac
     8281 ttaacggatt gggtggaaga aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa
     8341 caaacgcttc gttacggtga aaatagccat caagccgctg ccttttatca gtccgctctt
     8401 ctggaaaaat attcgatcgc agcagcaaaa caactccatg ggaaagaact ttcttataat
     8461 aatatccgag atgcagatgc tgcgctacgg atcatgaagg aatatgacga gccaacggta
     8521 gtcgccttga aacacatgaa tccttgtggt attgggacag catctacgat tttagaagcg
     8581 tgggaagcag cgtatgaagc agatcctgtt tcaatctttg gtgggatcat cgttttgaat
     8641 cgagaagtag atctgccaac agcagaacag atgcataagc tgtttctaga aatcatcatt
     8701 gccccttctt atacacaaga agctttggaa ttactgcaaa aaaagaaaaa catccgatta
     8761 ctgacagtag actttgcgca ggaagaaaat caaaatcgag aagaaacggt ctcagtctta
     8821 ggtggacttc ttgtacaaga tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc
     8881 gtgacaaaac gtaagccgac tccagcagaa gcaaaagcat tagcgtttgc ttggagagct
     8941 gtcaaacatg tgaaatctaa tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc
     9001 ggagccgggc agatgaatcg agtcggttca gtaaaaattg cgattgaaca agcgcaagag
     9061 aaaatggaag gtgcagtttt agctagcgat gcttattttc caatggatga ttcggttgaa
     9121 tatgcagcca aacatggtat aaaagcaatc attcagccag gaggaagcat caaagaccaa
     9181 gattctattg atatggcgaa taaatacgat ctagcaatgg tctttactga tgttcggcat
     9241 tttagacatt agagaggagt cggataaatg aacattttgg tgatcggtgc aggtggtcgt
     9301 gaacatgcaa taagtaaaaa acttttagcc agtccgaagg tggatacggt gtattgtgcg
     9361 ccaggaaatc cggggatgac aaaagatggt atcaattgtg tcgctatcag tgagcgagat
     9421 cattcagcgt taatccattt tgttaagaaa gaggctattg attggagtat cgttggtcct
     9481 gaagttcctt tgttaaatgg gatcgtcgat gattttatgg cggaaggatt gaaaatcttc
     9541 ggtccgacaa aagcagcagc tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg
     9601 gatacttatc aaatcccgac agctaaatcc agaagttttg aatgttacga aaaagcagca
     9661 gcgtatatca aagaaaatgg tgcgccaatc gtagtgaaag cggacggctt agcagctggg
     9721 aaaggtgtag tcgtggctac cacagtgaag gaagcgctgg atgcggcaga acaaatgttg
     9781 aaccagcatc gatttgggga tagtagcaaa aaaatcgtcg tagaagagtt tttggcagga
     9841 gaagaattct cccttttggc gtttgtccgt aataccaagg tgtatccgat ggtcatttcg
     9901 caagatcata agcgggcatt tgataacgat aaagggccaa atacaggcgg aatgggtgct
     9961 tattcaccag taccgcaaat ttcaaaaaag atgattgaaa aagctgtgga agaagtttta
    10021 aaaccagcgg ctttgggaat gaaagaacta ggacgctctt tcacgggtgt tttatatgcg
    10081 ggtttgattg ctacgaagga aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat
    10141 cctgaaacgc aagtcgttct ttctcggttg aagagtgact ttgcgcaagt aatcgatgat
    10201 ttattagaga atcgtacagt agagttaaag tggcagcaag aaggatacaa tgtaggcgtt
    10261 gtggttgcag ctgccggtta ccccgaagaa tatgagacag gaatggttct accagatttt
    10321 ctggaagaag agctttctgt ttactatgct ggagtttcct caaataacgg caaactagtt
    10381 ggagcaggag gacgtcttta tttagtagaa gcatacggtg agacgatcaa agaagcacag
    10441 aaaaaaattt acaccgcgtt aatggataaa acgacagatg gaacatttta tcgtacagat
    10501 attggagaaa aagctgccaa tagcgaactg atcgtgaagt aaatcagctg cttccattta
    10561 taaaaaatac aacaaacata atcggaagcg ccgattttag tgaagccttt tcataaagag
    10621 tgtggtacac ttttgttgtg tatataaata aggaggagtt cagatgaaaa aaattcaatt
    10681 aggtacttct gatcagaaag cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc
    10741 ggaagatcca gtaaaagtga ttgagacagc ttatgaaaac ggcattgatt tttttgacca
    10801 tgcagatatt tatggaaacg gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac
    10861 atcaatcaaa cgagaagatc tttttattca aacaaaatgt ggaatcgttc caggtgtgat
    10921 gtttgatttt tctaaaaaac atatcattga atctgtagaa aaaagtttga cccgtctgaa
    10981 aatggattat gtggatgctt tacttcttca tcgtccagat acattggtag aaccagagga
    11041 agtagctgaa gcgtttgatg aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag
    11101 caatcaaaaa ccaatgcaaa tcgaattgct gaaaaaatat gttcgtcaac cattagtagc
    11161 taatcagctg caatttggct tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa
    11221 tatgacagat gaagcaagta tcgatcatga cggtagtgta cttgattatt cccgattgca
    11281 tgatatgacg atccaagcat ggtcaccata ccagtatgga ttttttgaag gtgtatttat
    11341 cgggaacgaa aaattccctg aattaaacca aacattagaa gaactagcag ctaaatatca
    11401 aacgacacca actggcttgg cttctgcttg gattttacgc catccagcca atatgcaagt
    11461 gatcgccggt acgatgaacg tagggcgaat caaggaaatc gctcaagctt cagaaattaa
    11521 tatgtctcgg gaagattggt acgaattgta ccgtgctgct ggaaacattt taccttaaag
    11581 attgtcataa gcaggtcaaa agggctataa attaaaaaga cagaataact tgaaagctgt
    11641 tttcagcttc gagatattct gtcttttttt attgcaaatg gaaagagtat cattgtacgg
    11701 atttcgttta ttagttattt ttgtttttaa ggaaaagctg tgtccgatat tcttttggag
    11761 aaatcccttg ttttttctta aataaataac tgaaatagtt agggtcatta taaccaattt
    11821 caaaagcaat atctgaaata cgatagtctg attgctgcaa taattttttt gctaattcga
    11881 ttctttgttc tgtcagataa tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac
    11941 tgaaatgaga gggacttaat gcaacttgtt gagcgaccca agttaaggac atatctggat
    12001 cggtaaaatt ttctttgata aaatccaatg catggttcaa aattggctga tatttgttcc
    12061 ttgaaggctg tgagggcaga gcagttaaga aagaaatgaa catataaact gtcgaacggt
    12121 aaagcgagtc gtcggcgctg attgctaagc gttgactggt ggatgtgcaa tgaaccattt
    12181 cttgcgggat tttcttttct ttcaggcaga catgctggag tagcttagtc aagacaaata
    12241 aacgatataa tcgtgttcga gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact
    12301 tagatacttc gtttttcgat aatgaagaca aatcaagtga gaggtcaaag taaaagacag
    12361 aatcaactgg aagtttcgct tcttgaatca aatcctcata gctgataatt tgttctttaa
    12421 gggaactggc ggtttcaagt atcatctgcg tgatctcaaa agatgcaggt aattcgctga
    12481 ttctttctgc gacaggacca atggcgacag aaaaattatt tttttgtccc ttttctaatt
    12541 catggaccaa cgtttgggct acctgataac tggtctcgat cacattttct tcaagagaat
    12601 caactattaa aaatttgatg aatcgagagg aaacagcaga gaagatcact tgttgattct
    12661 ccctaaataa aaacttcaaa taatcactga gatgagcgta atcttcaaag tctgtactga
    12721 cttggtttgc tgctaataat acggtcattt ttttccctgc taaagagcga ttgaaagatt
    12781 cagcaagttt caatgtttca ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat
    12841 gatttttttt caattcgaaa acaagatcag aagaagtgtg cgtttctttt tgctgcaaat
    12901 ctaaagcagt tactgctttt tctattgctt gtttcaattc gctactttta atcggtttaa
    12961 gcagataatc atctgcctgt acctggattg ctcctttcat ataatcgaaa tcgtcgaatc
    13021 cactaataaa tatgagacga gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca
    13081 acccgtccat gaaaggcatt tggatatcag tcagaatgat gtcgggctgt aagtcgataa
    13141 ttgaagacag tgccatctca ccatctccag cttcgccgac ataagtgatt ggataataag
    13201 aaccaaggac agttatttgt tcccgcaagt tattgcgaat gaggtattcg tcttcgatga
    13261 taaaaacttt atacatgttt gtttcctcca attgttttgg gagatagagg gatcatcagt
    13321 gtgacagttg ttccttctcg gtatttgctg tcaatcgtga taggtttttg atcatttccg
    13381 taataaagaa ggagacgctt gtttacgttg taaagtccgt agccgatact gaaatctgaa
    13441 tcgatccctt tggctaactc tttacgtatt tctgccagtt tttctgcagg gatgccaatc
    13501 ccgttgtcta taactttgaa ttggatcatc tcatcaattt tgcatgtggt aatacgaata
    13561 aatcctttgt gacggacgta cttcgttcca tggtatacag cattttcaat caatggctgc
    13621 aagatcattt ttaaaataga aacaggaaaa agtgtttctg gtatatcgat cttatagtca
    13681 agcatatggc cgtatcttat ttttaagatg actagataat cattgatatg gctaatttct
    13741 ttttcaagag gaatccagtc ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt
    13801 gcatgggtca tagtcagagc ttcttcaaag cagtcttgtt cgatcaaaga aataatggca
    13861 tccaaactgt tataaataaa atggggagta atctgagctt gcaatacacg aacttcactt
    13921 tgtgctaaat ggtattgttt caaagcgttc tcccgtaaag actggtttag atcttccgcc
    13981 attttattca tgctagcggt aagtgtttgt aattccagtg tttccggcag ggttaaacga
    14041 aagccaaggt tccctgaaga aagttgttct gacatctcga tcagctgatt gatcggttct
    14101 tggacctttc tctctaaaaa gcgacgattc tttttcgcat agaaaaaaat ggtactaaga
    14161 actccaagtt ccactattgt taatagaatg agcgagcgga caagtgtcgt attttttttg
    14221 cttgctaagt cgatctccac acgaacaaaa tcttgtaaga tatctgacag cagttgagtg
    14281 acagaatcta cctgattgat gatctcttca ttggtatcca taaaatcttt tgccaagata
    14341 ttattgatca gctcgttttg ataatcttcc agtgtatcga tcgtacgtag cgatacatct
    14401 aacgtactaa tttctttaag tgtatttgta ttatttttga tctctcgaat atcattccta
    14461 agttcttcta ggatattttc ctcattgaac tcatctacgg ttacttggcc agaaatcaag
    14521 tcccacatac tttccaaaac tttttgatca accttcaaag caattgcatc tgctttttgg
    14581 atatttgcga tcgtctgttg ataaatcaaa agctgttgcg aatataacag tatgctcagt
    14641 agcaaaggga taacagcaaa tagaagcatg gctttgtttg cttgggcaaa aatctgtttt
    14701 atgcttaaat tttttgtcat gatacttctc cagtcaataa tttctggttg ggatgttagc
    14761 tatattctgc tgggaaaaga cagtctcgga aataaaaata tctttaggta atgtttttcc
    14821 tgtttgaata tttccaaaat aacgagtaac ggtattggct acataccagc cgacatcagg
    14881 tttacactcg actacacaat tgacgattcc tgagcgtagc ttctcgatca tatcttcttg
    14941 tccatcaata gttacaatga tcaaatcttc tccaggtttg attgatgttt cttttatcgc
    15001 agctaaagca ccgtgagtca tttcatcact atggctgtat aatacatcga tttcggataa
    15061 atcatttgtt tctaaatatc gtttcatctg gtcttttcct ttcatccgaa taaaatcacc
    15121 gacaattcgg tgaacaatag tcatcggagg tttatggtcg atggttccat ctttttttag
    15181 ggttggttta cgattgattg attccaaaaa tccttcggag cgtagcttgg tcggtgtgga
    15241 gttctctgaa ccagcaagtt ctaggattct gatttcctgt ttctttgaat ttgtgaagtg
    15301 attgctgaca aataaacccg cccgatttcc ttctgctttg aaactggggc caatgtgggt
    15361 gacgaaaagc tcgggatcat tcgtttgaat atgtctgtcg acaagtatga cagggatccc
    15421 tgctcgcttt gcttctttta atacagagtc ccacccggat tcttgcaaag gagtaaaaat
    15481 gatcatatcc actttatagg caatgaacgt ccgtatatct tggatttgcc gttcctgatt
    15541 catgtagcca tttcggtaga gaacttggta atcttctttt tcaagcgcag tgataattga
    15601 ttcagtatgc tttttccgcc agctactctc cgttcctgat tgcgaaaaac caacgactag
    15661 tgggtcaggt gcttcctttg tattaggtgt acaccccatc atttggtaag cgattacaac
    15721 aagggctaaa aaaaataaaa cacgtttcac tttaaatcac tccatctgga tttgttagta
    15781 ctatcttatc agataaaagg gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa
    15841 gaaacttaaa aaaatccaag acaaaaaaag tacacctaaa aaatttaaag taaatcccat
    15901 aaaaaaatca atgttaacgc tttctttatt agctatactc ggtgtatcaa ttgaaatggg
    15961 ggataaaaaa tgaaaaacaa atttagaaag attgttggga ctatcgctat tttgaccgca
    16021 agcattctat taagtgcgtg tggcaattca ggatctgcag atgatagtac ggggtatgta
    16081 ggaatcgcta tgccgaccaa atcagcagaa agatggatcg ctgatgggaa caacatggtc
    16141 tcggaattag aaaaactggg ttataaaaca gatcttcaat atggagaaga taaagtagaa
    16201 aatcaagtag cacaaataga aaatatgatt acaaaagggg tagatacact tgtgattgct
    16261 tctatcgatg gttcggcttt aactgatgtg ttagctaaag caaaagaagc agatatcaag
    16321 gtgatcgcct atgatcgttt actgatgaat tcagaaaatg tcgattatta cgcgactttt
    16381 gataattttg gagtaggtgt atcgcaagca gcttatattg aagagcactt aggattgaaa
    16441 gaaggaaaag gaccattcac gattgaatta tttggtggct cgcctgacga caacaacgca
    16501 ctaatcaatt acaacggagt gatgtctgtt ttacaaccat atatggacaa cggtcagttg
    16561 gtcgtgccat ctggacagac gagtttcagt caaatcgcta cgcttcgctg ggatggttct
    16621 acggcgcaag caaggatgga taatctgtta agcgcaaatt atactgacca aacattggat
    16681 gctgttttgt cgccatatga tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg
    16741 gggtatggga gtgaaagtaa acctttacct gtgattactg gacaagatgc gacagttgct
    16801 ggcgttaaat cgatcattgc cggggaacaa acacaaacga tctttaaaga cacacgtatc
    16861 ttagcaaaaa atacgattga aatgatcaaa gcaatcagtg atggagaaga ggttccggtc
    16921 aatgatactg aaacttatga taatggggtg aaaactgtcc ctacgtattt agcaaataca
    16981 gtatctgtcg ataaagataa ttatcaagca gagctgatcg acaccgatta ttataaagaa
    17041 tccgatctga aaaattaaga ggaaccaaac aaggaactaa agagatctaa gtcagagaga
    17101 ggataagaat tatggcggat tgtattttaa aaatggcaga tatcaccaaa aaattttctg
    17161 gaatccgagc gttagattgc gtgaatctga tgattgaacg tggggaaatc catgcattgt
    17221 gcggagaaaa cggagcggga aaatcaacat tgatgaatgt cttgagcggc ttgtatccat
    17281 atggctccta tgacggggaa atcttttatg atggaaagct gtgtcaattt aaagatttga
    17341 aagatagtga agaaaaaggc atcgtcatca ttcatcaaga attagcacta agtccttatc
    17401 tttcaataaa agagaatatt tttctcggaa atgaacaagc agaaagaggc gtgattgatt
    17461 gggaactgac agaaaagaaa acatatgaac tgctgaataa agtcggtttg tctatcgacc
    17521 cgaatcttct agtatcccag attggcgtag ggcaacagca actagttgaa atagctaaag
    17581 cattatcaaa atccgtccgt ctgttgattt tggatgagcc taccgctgca ttgaatgaag
    17641 aagaaagcgc gaatttgctg agattgatcc gagagttgaa agagcaaggt gtaacctcca
    17701 tcattatttc acataagctg aatgaaatcg tggatatcgc agatcgaatc accattttac
    17761 gggatggaca gacgattgag acactagaaa atgaaatgat cagcgaagag catattattc
    17821 gcggaatggt gggcagagat ttagttgatc gttatcctga aagaaaaccg gatattggag
    17881 aagtttattt taaagtagaa gactggacgg tctatcaccc tcttgatcac gagcgaatca
    17941 taaacgacca catcaacttg catttgaaaa aaggagagat tgtaggaata gctggactga
    18001 tgggagcggg cagaactgaa ttcgctatga gtctatttgg acattcttat ggaagcaaga
    18061 tatccggacg tatattaaag gaaggggcag agatatcagt taagaccgtt ccttcagcca
    18121 ttgcaaacgg actcgcttat gtctcggagg accgaaaatc tttaggactg aatctgctga
    18181 tggatattcg ggaaaacgtg acgctgagca gtttggataa aataagtcaa aaaggagtat
    18241 tagataaaga aaaagaagta actgaggcag aaaatttccg gaaaaagatg cgtataaaaa
    18301 caaactccat ccatcaaatt gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt
    18361 tatccaagtg gctgatgaca caacccgata ttcttttttt ggatgagcca acccgtggga
    18421 tagatgttgg ggcaaaatat gagatctaca cgattatcga agaaatggca gcaaatggca
    18481 aagctgtctg tcttatctct tctgaacttc ctgaaatatt agggatgtgt gatcgaatct
    18541 atacgatgaa cgaaggacgt attacaggag aagtctcgcg agaagatgca gatcaggaag
    18601 tcttgatgaa attaatgacg aaagaagagg tgggttaagt tggaacaggc aaaaaatctt
    18661 acggataaaa aagagaaaaa actaaatttg aaaagtagag cagctgatct ttttggtcgc
    18721 tacagcatgg tgatcatttt agcgggacta cttattgcgt ttcaattgat gacggatggt
    18781 attttctggc gtccattgaa tataactaac cttgttctgc aaaatagcca tatattagta
    18841 ctagctgcag ggatgttatt agttgtgctc ttaggtcatg tggatttgtc ggtagggtca
    18901 gtgatggcgt ttgttggtgc aattgcgggc atgatgatgg tcaatcgcca aatgaacccg
    18961 tggatgacag ttatcgcatg tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt
    19021 tgggtagctt atgcagggat accggcgttt atcgtgactt tagcaggatt gctgatgttt
    19081 cgtggactga cacaagttgt tctaggaggc caatctctag caccttttcc agctgttttc
    19141 caaaaaatgt ctacgggtta tttaccagac ttaagcgaag gaagcagcct tcacttcttg
    19201 acattgattt taggaatgat catcacgatc gtgttagttg ttggtcaatg gcgaacaaga
    19261 aatcgtcgga aaaatcattt atttgaagtg gaatctccaa cagctttctt ggtaaaggcg
    19321 gtatttacag cagtcgttgt actaggaata acttatatct ttgcctctta tcaagggtat
    19381 ccgattattt tgatcattct tggtttgatc gttgcagcat atgggttttt gacaaataaa
    19441 acagtagcag gtcgacagat ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca
    19501 gggatcaaaa caaagcgaat cacgttctgg gtattcgtca acatgggagt catggcagct
    19561 ttagctggat tagttttagc tgctcgattg aatgcggcaa cacctcaagc aggtacatcg
    19621 atggaactgg atgcaatggc ggcagtatat tttggtggtg cttcgacttc cggcggcatt
    19681 ggaacaatca tgggtgcgat tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg
    19741 tcgattttag gcgtcggggt agactggcag caggcaatca aaggattgat cttgctttta
    19801 gccgttgtgc tagacttgta taataagaaa aaacgaacaa cgtaggagaa cgccggttat
    19861 tcagaaaaaa gactgtgaca agacatttgt cacagtcttt ttttcgctaa aagcgaacat
    19921 tcattgtttc gtttgtatag actaaaaata atatatgttt ttttaccatt cgtataaatt
    19981 ctctttttgc tcttgatttt tttgcttaat tgagttaaat tacatagggc gctaaaaaaa
    20041 gccttgatta aattaataat gttcgtgttt tgctggatgt gaacatcagg aggataatgt
    20101 aatgaaagta tttgattacg aagatattca attaattcct aataaatgta tcgtaaatag
    20161 ccgttctgaa tgtgatacaa cggtcacatt aggtaaacat acattcaaaa tgccagtagt
    20221 gcctgccaac atgcagacga ttattgatga aacaatcgca gaattcttag cggaaaatgg
    20281 ttatttctat atcatgcatc ggtttgatga agcagctcga attccgttta tcaaaaaaat
    20341 gaaaaagcgt ggattgatta cctcgatcag tgttggcgtc aagaaagagg aatattcctt
    20401 tatagagaag ttggcagaag aatcgctaaa tcctgattat atcactatcg acatcgcgca
    20461 tggacatgct aactcagtta ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac
    20521 ttttgtgatt gccggtaatg ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg
    20581 cgcagatgca acaaaagtcg ggattggtcc aggaaaagta tgtattacaa aaatcaagac
    20641 aggctttggg actggaggct ggcaactggc agctcttaga tggtgtgcga aagcagctcg
    20701 caagccgatc atcgctgacg gtgggatcag aacacatggc gatattgcaa aatctgtccg
    20761 ttttggtgcg acaatggtca tgattggttc attgtttgcc ggtcacgagg aatctcctgg
    20821 cgaaacaaaa gtcgaaaatg gaatcgtgta taaagaatac tttggttctg cttcagaatt
    20881 ccaaaaaggc gaaaaacgaa atgttgaagg aaagaaaatc tggatccaac acaaaggatc
    20941 tttgaaagat acactagtgg aaatgcaaca agaccttcaa tcatctattt cttatgctgg
    21001 aggaagagat ctggaagcca ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt
    21061 taatggagac acaatctaaa aaataaataa aatattagga tgttgtgaaa aagttgtcct
    21121 gcattaagta ataagaacag ccaaacaaaa atagttcttt atatgttttg tttggctgga
    21181 attattatcg cagatacaag cttttgaaca tcattattca gaataaggga ctgtgataag
    21241 atttttgtca cagtccctta tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg
    21301 gtaaaatcaa aagaaaaaat agacaataga aaagagaagc ttatgtggga aaaagtagag
    21361 aaaaaacaag gaccgattta tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac
    21421 ggaaggatca atcctggaag tcaacttgaa tcagaacgaa aactagggca actttttggt
    21481 gtgaatcgtt ccaccgttgt ccatgcattg gaagagctta gggagcttgg ggtactaacg
    21541 agcaaacgtg gcagcggatg gtttgttaat cagactgaat gggggaaatt cgctgttcca
    21601 agaatcgatt ggcggcaaat gatctctcct cgttatgagc agacggactg gtatgagcaa
    21661 aaaataagag aagcaaagca gatccaaaaa acatcgtttc ttgatttata cgctagcgag
    21721 atgccagatg agcttttgcc gaattttgag tttccttctt attctcttga agaaatctta
    21781 gcggaagaaa aagaaatgga tcttttaggc tatactccgt tacgtaacaa aatcaaacgg
    21841 tatttggaaa gtaaatttga attggcattt tcccccaacc agcttcttgt gactggtggc
    21901 ggtcagcaag caatcttttt gattttacaa acgattcttt cagctggaga ggggatagct
    21961 gtagagtcac cttctttttt ttaccgatta cctcttttca aagcttcagg aacgcgttta
    22021 tttgggatac cgatggatga agaagggatc gacttagaaa aactgaaaga atcgatccat
    22081 aaaaataaat tgaaagcagt gcttatcaac ccgaattttc aaaaccctac aggaaaagtg
    22141 atgagtcaaa aacgacgaga aaagctagta gaattgtgtc gacagtatcg gcttccaatt
    22201 attgaagacg atgtatttac ggatctttca tttctaaacc aagaagaagt ccttcctata
    22261 cgaacgattg atccggataa tgtgttatac gtcggttcgt tatctagggt tttaggaaaa
    22321 acaacgaaaa tcggttgggt gataggacct gagactttta tctcgcaact agctcaagcg
    22381 caagaaatga tggagttttc gatgaatatc cttacacaaa tcacagtggc taatgtattt
    22441 aatgaagcaa ttgatttgaa aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga
    22501 ctgataagcg cttggggaga ttcacaatcg tttttcaagc ttcattcaat tttaggtggt
    22561 tactatgcct ggattacttg ggatgggaaa aaactagacc ccgaactagc agaaaaagta
    22621 gtagatatgg gtcttggaat tgcaccaagt ctactttttg ggcaagatat aaatggaatc
    22681 agaatcaatt acagtaggct ggatgaaaac caaataccgt tgttttattc aaagatgaaa
    22741 attctagaaa agtggttaac agaataattt cacaaagtaa atagattaaa gaatataaca
    22801 gcttggattt aaatttcaag ctgtttcttt tttttcggaa aaagaatacg atataaagaa
    22861 actaagaaaa aaccattatt tgtacgtccg ttcacgatgt gtaaataaaa taaatgtttg
    22921 caacaaaact gtataacacc aacgttttta aatagaaaca gatggttgta agtttgatta
    22981 gaaaatagaa aaaatgcaga tgaaatataa aacgctttcc ttattgtgat tttattcact
    23041 aatgctgtta atatgcggat tggcataatt tcctttcatc gtgaaaattt gtgcttttag
    23101 cttgtatttc tcataaatcc atgataattt attaatgaag acggttaaat agtgatacag
    23161 ttttattcac tgtttcaatc gcagaatgaa gagattttct tatagatgag agaggaatgt
    23221 gtgacaaatg gcaaacaaaa aagcaccgat cgacttccaa gcactgctag atgaagtcaa
    23281 tgcagatttc ccagtatatc aagcacttga tcaagatgga aacgtagtta acaaagattt
    23341 agtacctgat ttatccgatg atgaactcgt tgaattaatg actcatatgg tatggtctcg
    23401 tgtattagac caacgttcaa cagcgctgaa ccgccaagga cgcctaggct tttttgcacc
    23461 aactgctgga caagaggcaa gtcagctggc tagtgcttac gcatttgata aagaagatgt
    23521 tcttcttcca ggttatcggg atgtacctca actagtaaaa catggtttac ctttatctca
    23581 agctttttta tggtctcgag gacatgctgc aggtaattct tatccggaag aattgaaagc
    23641 tctaccacca caaattatca tcggtgcaca atatgtacaa gcagccggtg ttgcattagg
    23701 tttgaagaaa cgaaacaaga aaaatgtcgt cttcacttat actggtgatg gcggttcatc
    23761 acaaggtgat ttttacgaag caatcaattt cgctggcgct taccatgcaa atgccgtatt
    23821 ttatatccaa aacaacgggt atgcgatctc tacacctcgt gaaaaacaaa ctgcagcaaa
    23881 aacacttgca caaaaagctg tcgcagctgg gattcctggt attcaagtcg atggaatgga
    23941 tccgcttgca gtatatactg tatctaaaat ggcacgggaa tgggcgatca gtggaaatgg
    24001 accagtccta atcgaaacac tgacttatcg ttatggacca catacactat ctggtgacga
    24061 tccaacccgc taccgtacaa aagacacaga tgacgattgg cagaaaaaag atccattgat
    24121 ccgcttccgt aaatatttga cggaaaaagg actttggtct gaagaaaaag aagaacaagt
    24181 catcgaagca actaaagaag aaatcaaagc agctattgct gaagccgaca aagtaccaaa
    24241 acaaaaggtt tcagatttct tgaaaaacat gttcgaagta tctccacaat caatcaaaga
    24301 acaaattgca atttatgaag cgaaggagtc gaaataatca tggcacaaaa aacaatgatc
    24361 caagcaatca cagatgcctt agcacttgaa ctagaaaatg acgagaatgt cgttgttttt
    24421 ggtgaagatg ttgggaaaaa cggaggagtt ttccgggcaa ctgaaggatt gcaggaaaag
    24481 ttcggagaag accgagtatt cgatactcct ttagctgaat ctggaatcgc aggattgagt
    24541 ttcggtcttg cgttagaagg atttcgtcca gttcctgaaa tccaattctt tggttttatt
    24601 ttcgaagcaa tggacgaagt agtagctcaa atggctcgta caagatatcg aatgagcggc
    24661 actagaaatc tacccatcac aattcgttcg ccatttggtg gcggtgttca tacaccagaa
    24721 ttgcattcgg ataatttaga aggtttgatc gctcagtctc ctggtattcg agtagtgatt
    24781 ccttccaacc cttatgatgc aaaaggacta ttgatttctt ctattagaag taacgatccg
    24841 gttgttttcc tagaacacat gaaactatat cgctcattcc gagaagaagt gccggatgaa
    24901 gcttacgaag ttcctttgga taaagcagca gttactcgtg agggaacaga tgtatctatt
    24961 atcacatacg gagcaatggt gcgtgaagca atcaaagcag cagacaatct tgcaaaagaa
    25021 aatatatctg ttgaaatcat tgatcttcgt acagttgcgc cattagatgt agaaacgatc
    25081 attcaatccg tggaaaaaac tggtcgcgta gttgttgtcc aagaagcaca acgacaagcg
    25141 ggcgttgccg cgcaagtagt ttctgagatc tcagaacgcg ctatcctttc attagaagcc
    25201 ccaatcggac gagtttctgc acctgataca gtcttcccat ttggacaagc agaaaatgtc
    25261 tggttgccaa atgcaaaaga tatcgaagac aaagtaaaag aaatcgcgga attttaagaa
    25321 aagaagggac gaaacaaagt catggcttat caatttaaat t
//
LOCUS       DACDYJ010000039        24577 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDYJ010000039 DACDYJ010000000
VERSION     DACDYJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.039"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(152..550)
                     /locus_tag="IWM51_001644"
     CDS             complement(152..550)
                     /locus_tag="IWM51_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP9971068.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="IWM51_001645"
     CDS             complement(547..1320)
                     /locus_tag="IWM51_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9971069.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="IWM51_001646"
     CDS             complement(1317..2000)
                     /locus_tag="IWM51_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9971070.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="IWM51_001647"
     CDS             complement(2167..3054)
                     /locus_tag="IWM51_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9971071.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="IWM51_001648"
     CDS             complement(3051..3920)
                     /locus_tag="IWM51_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9971072.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="IWM51_001649"
     CDS             complement(4057..5343)
                     /locus_tag="IWM51_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9971073.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="IWM51_001650"
     CDS             5616..6194
                     /locus_tag="IWM51_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9971074.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="IWM51_001651"
     CDS             6194..7123
                     /locus_tag="IWM51_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9971075.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="IWM51_001652"
     CDS             7120..8856
                     /locus_tag="IWM51_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9971076.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="IWM51_001653"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="IWM51_001653"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP9971077.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="IWM51_001654"
     CDS             complement(9752..10261)
                     /locus_tag="IWM51_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP9971078.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="IWM51_001655"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="IWM51_001655"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP9971079.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="IWM51_001656"
     CDS             complement(11562..12653)
                     /locus_tag="IWM51_001656"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP9971080.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="IWM51_001657"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="IWM51_001657"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP9971081.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="IWM51_001658"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="IWM51_001658"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP9971082.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="IWM51_001659"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="IWM51_001659"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP9971083.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="IWM51_001660"
     CDS             complement(16616..17590)
                     /locus_tag="IWM51_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP9971084.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="IWM51_001661"
     CDS             complement(17571..19454)
                     /locus_tag="IWM51_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP9971085.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="IWM51_001662"
     CDS             complement(19472..21697)
                     /locus_tag="IWM51_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9971086.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="IWM51_001663"
     CDS             21812..22846
                     /locus_tag="IWM51_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9971087.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="IWM51_001664"
     CDS             complement(22913..23926)
                     /locus_tag="IWM51_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9971088.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="IWM51_001665"
     CDS             complement(24004..24192)
                     /locus_tag="IWM51_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP9971089.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7063 a         5341 c         4397 g         7776 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tacaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccgc tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc gcaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24541 tattggcaaa tcgccaaaat aatgtagact aactaaa
//
LOCUS       DACDYJ010000040        24272 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDYJ010000040 DACDYJ010000000
VERSION     DACDYJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.040"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..572
                     /locus_tag="IWM51_001666"
     CDS             <1..572
                     /locus_tag="IWM51_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9971090.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="IWM51_001667"
     CDS             576..1217
                     /locus_tag="IWM51_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9971091.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="IWM51_001668"
     CDS             1214..2131
                     /locus_tag="IWM51_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9971092.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="IWM51_001669"
     CDS             2128..2799
                     /locus_tag="IWM51_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9971093.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="IWM51_001670"
     CDS             complement(3340..3828)
                     /locus_tag="IWM51_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP9971094.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="IWM51_001671"
     CDS             complement(4029..4295)
                     /locus_tag="IWM51_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9971095.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="IWM51_001672"
     CDS             complement(4295..4558)
                     /locus_tag="IWM51_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9971096.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(4551..4823)
                     /locus_tag="IWM51_001673"
     CDS             complement(4551..4823)
                     /locus_tag="IWM51_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971097.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            5034..5219
                     /locus_tag="IWM51_001674"
     CDS             5034..5219
                     /locus_tag="IWM51_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9971098.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5361..7184)
                     /locus_tag="IWM51_001675"
     CDS             complement(5361..7184)
                     /locus_tag="IWM51_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP9971099.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="IWM51_001676"
     CDS             complement(7782..10487)
                     /locus_tag="IWM51_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP9971100.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="IWM51_001677"
     CDS             complement(10793..11740)
                     /locus_tag="IWM51_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP9971101.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="IWM51_001678"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="IWM51_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9971102.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="IWM51_001679"
     CDS             complement(12375..12737)
                     /locus_tag="IWM51_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9971103.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /locus_tag="IWM51_001680"
     CDS             complement(12748..13869)
                     /locus_tag="IWM51_001680"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP9971104.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /locus_tag="IWM51_001681"
     CDS             complement(13885..14235)
                     /locus_tag="IWM51_001681"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP9971105.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="IWM51_001682"
     CDS             complement(14365..15876)
                     /locus_tag="IWM51_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9971106.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /locus_tag="IWM51_001683"
     CDS             complement(16198..17550)
                     /locus_tag="IWM51_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP9971107.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="IWM51_001684"
     CDS             complement(17703..18779)
                     /locus_tag="IWM51_001684"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP9971108.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="IWM51_001685"
     CDS             18975..20720
                     /locus_tag="IWM51_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP9971109.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="IWM51_001686"
     CDS             complement(21000..21437)
                     /locus_tag="IWM51_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP9971110.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="IWM51_001687"
     CDS             complement(21421..22794)
                     /locus_tag="IWM51_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971111.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="IWM51_001688"
     CDS             complement(23116..23604)
                     /locus_tag="IWM51_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9971112.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="IWM51_001689"
     CDS             complement(23626..24228)
                     /locus_tag="IWM51_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9971113.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6960 a         5164 c         4486 g         7662 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agctcaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgtc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcgt aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctt caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa acttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc acggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctcc tt
//
LOCUS       DACDYJ010000041        24109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDYJ010000041 DACDYJ010000000
VERSION     DACDYJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.041"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            197..3487
                     /locus_tag="IWM51_001690"
     CDS             197..3487
                     /locus_tag="IWM51_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP9971114.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESCTYT
                     NAQEGFALMVRDAVPKDHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKDLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIKLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSFLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTVSKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNESPNESPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVV
                     YVMKQRKK"
     gene            complement(4027..5934)
                     /locus_tag="IWM51_001691"
     CDS             complement(4027..5934)
                     /locus_tag="IWM51_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP9971115.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNEQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIQYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6064..6939
                     /locus_tag="IWM51_001692"
     CDS             6064..6939
                     /locus_tag="IWM51_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP9971116.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     ALIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKEWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            7019..7993
                     /locus_tag="IWM51_001693"
     CDS             7019..7993
                     /locus_tag="IWM51_001693"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9971117.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8182..9318
                     /locus_tag="IWM51_001694"
     CDS             8182..9318
                     /locus_tag="IWM51_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9971118.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSCQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLEQLVRAVKK"
     gene            9455..9799
                     /locus_tag="IWM51_001695"
     CDS             9455..9799
                     /locus_tag="IWM51_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP9971119.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10070..11343
                     /gene="efmA"
                     /locus_tag="IWM51_001696"
                     /pseudo
     CDS             10070..11343
                     /gene="efmA"
                     /locus_tag="IWM51_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
     gene            complement(11493..12092)
                     /locus_tag="IWM51_001697"
     CDS             complement(11493..12092)
                     /locus_tag="IWM51_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP9971120.1"
                     /translation="MNYFTSDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12496..13617
                     /gene="mnmA"
                     /locus_tag="IWM51_001698"
     CDS             12496..13617
                     /gene="mnmA"
                     /locus_tag="IWM51_001698"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP9971121.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNHAEVVFD
                     EPVRAITPGQAVVFYDEMECLGGGLIDHAYQETKVLQYV"
     gene            13863..15284
                     /gene="arcD"
                     /locus_tag="IWM51_001699"
     CDS             13863..15284
                     /gene="arcD"
                     /locus_tag="IWM51_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP9971122.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFAIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVAFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15298..16416
                     /locus_tag="IWM51_001700"
     CDS             15298..16416
                     /locus_tag="IWM51_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP9971123.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SEFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILER
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16476..17768)
                     /locus_tag="IWM51_001701"
     CDS             complement(16476..17768)
                     /locus_tag="IWM51_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9971124.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLAHQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNAAALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLMGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSISSGHSPSHRQ"
     gene            17918..18478
                     /locus_tag="IWM51_001702"
     CDS             17918..18478
                     /locus_tag="IWM51_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9971125.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLEGIHETNQIILQIFEAFQLTKEEKVHRSRELCSMIHGYLSLRFLG
                     YFTKESTIDPEDSYFWMINDFIATLP"
     gene            18607..18984
                     /locus_tag="IWM51_001703"
     CDS             18607..18984
                     /locus_tag="IWM51_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP9971126.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEAEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESGKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18917..20989
                     /locus_tag="IWM51_001704"
     CDS             18917..20989
                     /locus_tag="IWM51_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9971127.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEEQKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKEMRINPDF
                     TIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKMLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21200..22147)
                     /locus_tag="IWM51_001705"
     CDS             complement(21200..22147)
                     /locus_tag="IWM51_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9971128.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDVDSVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22392..22856
                     /locus_tag="IWM51_001706"
     CDS             22392..22856
                     /locus_tag="IWM51_001706"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP9971129.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQQILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22918..23961)
                     /locus_tag="IWM51_001707"
     CDS             complement(22918..23961)
                     /locus_tag="IWM51_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP9971130.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGIKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         7826 a         4416 c         5068 g         6799 t
ORIGIN      
        1 tgctcttctt tatacgttat tttaaaaaaa tcttattctt tttacgttaa taaatctctt
       61 tgttatcaac aacttaagtt atgttttttc tttttataaa atacacttat tataattttg
      121 ttttttattg acagtaaaaa taaattaggt aatactttaa gtaaacgttt tctaatttgt
      181 tgcgggagga atacacttgt taaaaaaacg tataaaatac agtgcgctaa tgatgaatct
      241 ccttatgctc agtaccccgg ttttaatttc tgtcgttgaa gttgctgcta acgaacaaca
      301 gtctgtgaac gaagaaaatg cacacgtgaa cattgaaggg atgtcaacaa tggaggaagt
      361 accaagaact agtgaagata tggatacgga agaaggaaat gatcctatga tgaaagaatc
      421 agaaaatgaa aagacaaaaa agcaatctga agaagcaaat gaagagtgga aaggggttgt
      481 tttcggagaa agcacaagtg ctagtaggta tgccattgaa ccattagaag acggcgtgcg
      541 gctctcttcc acgaataacg gcggaaaatt ccagtcaagc ggatcagatg gaatgaccta
      601 ttattatcaa gcaatcccca aagacatcaa ctttaggatg cgggcgacga tcgaaataga
      661 atcttgcacc tatacgaatg cccaagaagg atttgcttta atggtaagag acgcagtacc
      721 aaaagatcat ttgtttgggc aggcatttta ttcaaattca tttgccatac taggcagtcg
      781 gatcgaatat gtctgggact cggatagaca agaagtggtc aacacgagcg gcagcaaata
      841 tacgatgcga cttggattgg ggacacgggc aatcacagga atatcatctg aagatccgca
      901 agctgcgcca gctcctggga gtgtctcagt ggaaacaaca cctttagaga cttcagcagg
      961 gttcttggaa aaagaaacag gttcttataa tatattcgga aacggtcatg ggaatctgtt
     1021 tccggccacg aactcgatta ctaaactgga cgtagagatg aaaaaaacga atactggttt
     1081 agaggcatat tattacgatc ctgaaacagg agaagaaatg gcttctgaca tcatgtatga
     1141 ctgggaaaaa ttatacgctt cagatgaatc ggttatttat gcaggatttg ctgcagcaag
     1201 gaatatgacg atcaaggtag aaggaatcga agttgcttac tcagatcctg cgacagatcc
     1261 tcctgggcaa gaaagaccta ctcgcttgat tgatccgatt tacactgtga cctctagtaa
     1321 tcattcagga aaccaagatc atactatgtc ctttagagca aatgctgacg gattattgac
     1381 aattaaaaat aaagcaacag gagaggtgct taagaacgca aaagacttgg ctatcactgc
     1441 gaatcgggaa tttgattatc agacaaaact gatggaagga acgaacgaat ttactttcag
     1501 ttttacgcca gataaaaact atccgccaga agaatacgta gagatgacta gttatgaaac
     1561 aaaagagatt cttcatacag tggactaccg taaacctaaa tattcgacag tttatgtaac
     1621 accagaaggg tcagatgcag gaaacggaag cagacaaaat ccattatctc tgacacaagc
     1681 attgagatac gcatatgctg ggcagacaat cgtcatggcg cctggaactt attccttcga
     1741 aagaggattg acgattccca aaggcgttaa cgggacaaga gaaaacccga ttaagttgat
     1801 tgcagagaaa aatccagaaa acaagcgtcc tgtacttgat tttaaaggaa ctggaaatgg
     1861 gatgacactg ttcagtcact actggggttt acgagggttt gacattacaa atagtgcagc
     1921 tatgcaaaaa ggattacaga tttctggaaa tcataatttg atcgaagaga ttcatgctta
     1981 tcggaatgga aatacaggaa tccaaatcag cggtagctca aatgatcctt tcgaagcatg
     2041 gcccgcgcat aacttagtca agaactgtac atcttttgaa aatgccgatc caggatttga
     2101 agatgccgat ggatttgcgg ccaaactgac aataggagaa ggaaatgtat ttgacggttg
     2161 tatcgcgtat cataatgcag atgacggttg ggaccttttt gctaaaaatg aaacaggttc
     2221 gatcggaaaa gtaatcatca aaaactcagt tgcttatcga aacggctggg taccgggaat
     2281 tgagggagaa ggtaacggta atggattcaa aatgggcggt tcttttttaa cgggccctca
     2341 tgaattgatc aatagtttgt ctttcgaaaa tcttgcgaaa ggaatcgatt ccaatagcgg
     2401 aacagatatt atcgtcaata gcagtacaag ttttaataac ggttcatata atgtggctct
     2461 ttatacctct tcagcaggaa acacagacta ccatgcttct ggtatcttat ctttccgaac
     2521 agagaatctt gagatgagag agcagatccg cccattgaat caaaacgaag atcaaatcta
     2581 ccactcgttg aactactatt ggaatgaacg agagaaacaa tcagaaaata ctcttggaca
     2641 taccgtatca aaagattgga tcgagtctct aaatacatgt tcgaaagaag gtcagcagcc
     2701 tgttacgcgc tatgaaaatg gaagtatcca tgttcatgga ctgatgcaga ttaaaccggg
     2761 gaatcgtcaa acagaaaagg aaagagtgga tggtcttcca ttatctgtag gtgctatttt
     2821 tgacggagaa acctcgccat cttcggaata tccagagtat gcaatcgtac aagaaccaaa
     2881 tcagacaaaa ccaaatgaac aaaataatcc gattattata caagaagcaa atggtgtggc
     2941 ggagaatgca gaaggaaata tcattgaact gaagcctttt acagtttttg tgtcaacttt
     3001 cacaccaaat caaggaacgc ctgtctttga agatatgcgt tatttcccag aaaagatcca
     3061 aatcgttgaa acacagtcaa gtgctcaatt ttctaatcat accgctattt tttcagcacc
     3121 ttcaaaagga gaatatacgc tagaagtcac ttatcgttcg gaaatatttg atggggaaaa
     3181 atggtctgct catacagaat ctgttaccca gcaaaaagcg atcactgtgc gagaaactgc
     3241 cgatgtgccc ccgggaaatg acaaaccaga aagagaaaat ccaggaaatg aatcaccaaa
     3301 tgaatcacca aatgaatcac caaatggaaa accttctcat ccggacagga cagcaaaaga
     3361 aagtgaaaaa aatggcactc aaggactccc aaaaacggga gaatttaata acccgttgct
     3421 agcattagct ggaggaattt tgttaatcgg tgtagttgtt tatgtcatga aacagcgaaa
     3481 aaaataagtg gaatttctcc ttttatgatg taaaacaatt gactgaggaa agtaaaaata
     3541 gaaggaaatg agaccaatag atgtctcact tccttctatc tttttataga tgaggttctc
     3601 cttgctgctt ttgattggct gttcttatta cttgatgcag gacagctttt tcacaacctc
     3661 tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaacaaac ggcaaaatat
     3721 gaggaactat ttttccgttt tttctctaat actcaggtca ggacgctgat ttcacaacct
     3781 ctcgataaac ggtgttcaaa agccagtttc tcagtagtaa gtcaaattta tgcaaaaatg
     3841 tggaaagcta ttttcacaaa atttttctta ctacttgaaa ctaagcggct ttttcacaac
     3901 ctctgataca tggtattcca gaagtaactt cttcgaaaaa taagccgaaa tgtcacaaaa
     3961 atttgagaag caattttccc caattccttc ttatttcttg aagctgacca cttctggcac
     4021 aacctcttac cagattcgca ctcttttttc tggcgctaga tacatagcgt cttgttccgt
     4081 gataccaaat gtttcataga attcgtcaag gttttttggt tgcacgtttg ctcggagttt
     4141 agcaggggca tggacatcga tttgcagtaa aagttgttgg tattcttttt ttgcttttgt
     4201 acgccaaatc gttgcccaat tgacaaagaa ttcctcgata gaaagatctg attccgtttt
     4261 tgctgcttcc aatgcacagc ttagaccgcc tgcatcagca atattttccg aaacggttaa
     4321 ttttccgttt acttttccgt cagcaaaagg cagaccatcg aattcttcga tcattgcttg
     4381 tgctttgttt tgaaattgtg taagatcttc ttctgtccac cagttgttca agttgccata
     4441 ttcgtcaaat aaagcgccgt tattatcaaa tgcatgggaa atctcatgag cgataacagc
     4501 tccgattcca ccataattag cagagcttga ttgttctaga ctgtaaaatg gtgcttgaag
     4561 gatcgctgcc ggaaagacga tcacattccg aaatggatga taataagcat tgaccgtgtc
     4621 agcactcatt tcccattctg aacgatccac tggtttgttc catttgctga aacgatcttt
     4681 taatgtgagt ttgctgaaaa caagagtatt tgaaagcaaa gtaccaccct gatctgcagg
     4741 tactgttttg aactggtcga ataatgctgg gatcttatcg gggtagccta cttggatacc
     4801 taagttattt aattttatga cggctttttc ttttgttgca tccccaagcc agtcattttc
     4861 ttctagacgt tttttataaa cggcgatcat ttttttcacc atttgttcta cgtctttttt
     4921 agcagcttcg ccaaaatatt tttttccgta gtaatcgcca actacttgat cgaattgtcc
     4981 agaggcaagg taatacgctg cttttttctg aggcattgct tcgtctgttc cagacaatac
     5041 gcgagaatag attccgctga cttggcggaa ttcttctgat agataaccac taagagaacg
     5101 tactgtttta atgatcatcc agcttttgaa taacggaaaa tgagcttctg tcaggatgtc
     5161 attcaaatga tcgaagtaca ctggatcagt tacgatcact ttatctggag ttgtcccaat
     5221 caaacccgtg acaagttttt tcaagtccag tttatctgtg tattgaataa atgtatcaaa
     5281 agattgcgga ttatacattt tgctgtaatc tgcgttttct tctgcacttt ttacatgagg
     5341 agcaatcaat ttatcaaatt gcactgcttg ttccgtgatt gcttctgcct gacttttttc
     5401 tttgccggca agctctaata actgaacagc catatcaaag aaaacgccta ttagttgtgc
     5461 accgttggga tggtctgatg cataatacgt cttatctgga aggaataaag aaggcggata
     5521 agcaaagaag gcgttcgttt gcgcattctt catatctgca tctacatcca gattaaatgg
     5581 caatggtaat gtatccaaca gccattctgg aaattgctca ttcaattcgt cgaaagactg
     5641 caatgattca actttttcga ttataggttg aagtggttga gcatgcaact catttcttcg
     5701 gtcataatcg tttgccagtt gataatatgc aaggaaatgt ttcatttttt ctgaagacac
     5761 tttttctgga tgggcaagca gatcgtctgt ttctcccatc aaaagttggt cgatcccgtc
     5821 aactaaatct tggaaaccgc ctgtagctgg tttatctgca gggatttttg ctgttttttg
     5881 ccattcttca ttgactgctt catagaaatc ttgtttcaat cgttctttgt tcattcatcg
     5941 atcgctcctt ttttgtttcg tgtgcccatt atcttacaaa atgaaggaat taacaaatag
     6001 aacataaaaa attgaaaatt cgaacaatca aactgatttg tttttttcaa tccttcactt
     6061 ataatgaaaa aaaggaggcg atcccaactg aatcaagtag tggataaacc tttatatttc
     6121 aaagtagata aaaaaattaa aaaattagcc agcactcagc agctgcagtc aagaaaaagt
     6181 aaattgcttt ttttagctct ggtttttgaa gatcaaagtt atgtgattat cgatcaatca
     6241 ggacatccgg tagaatattc tccagcaaaa tatacttatc aagagggtct ttcttgtaaa
     6301 aaatggaagc tgatcaatga agagccaatc gagttatcta gatggatcaa cagaaaagaa
     6361 gatattcccg ctttgatcga agaaaaaagg tcaggaaagg aactcgcaaa ttgctgggtg
     6421 ggattgccag aagaacgttt tttgaggtat aagaaatggg ctaccccctc aggctatctt
     6481 tgtggtactt acgcggcagc agttctcctt gcctattatc aagactatcg taaggagtgg
     6541 atgcttccaa atgagattcg caagaaaaat acggctgatt cgttggtttt gacgaaggca
     6601 ttgcgtagcc agatccaacc actaggactt cctacgattc cttttcaggt tagtacaggg
     6661 atttctagtt ttttaaagaa gaatggcaat catgagcgtg cacgagcaac tcttctaggt
     6721 tcatggcagc gagctacaaa acgtatacgt gaaggcaaac ctgtgatgat tggtattttg
     6781 aaagtacttg gcagtacata tggcaatcat tgggtgacag cgtacgctta ttttgaaact
     6841 gaaacaggag aacgttatta caaagtacac gacaactggg gcgattatca taaagtcatt
     6901 cctgccagtt ggagtaacgg aacagtttca cttccttaag aatttgctga aattaacaga
     6961 aaatcttgga aaaatatgtt aaactatcga agaatagaga agttttgggg aggactacat
     7021 gagtaataag tatcaggatg acacgttgaa gatttttagt ttgaatggca accgtccatt
     7081 agctgaaaaa atcgcgaaag tgttcggaac agaattaggt aaaagcgtcg tgaagcaatt
     7141 tagtgatggc gagatttcca tcaatatcga agaaagtatt cgaggggatc atgtgtatat
     7201 cgttcaatca acgaatgcac ctgtcaatga ctactatatg gaactactga tcatgatcga
     7261 tgcaatgaag cgagcaagtg cgaaaacgat caatgtcgta ttgccttact atgggtatgc
     7321 taggcaagac cgcacggcga aacctcatga accgatcact gcgaaattga tcgctaactt
     7381 gatcgaagaa gcaggggcaa ctcgagtact gactttagat ctgcacacgg ttcaagtaca
     7441 aggattcttt gatattcctg tagataattt atttacgatg ccgttgtttg ctcattatta
     7501 ccgtcaatta ggtctgacgg gagatgacat cgtagttgtt tcgccaaaaa acagcggtgt
     7561 tcagcgtgca cgtagtcttt cagaatattt gaacagcaca ttagccatcg tagatcatgc
     7621 agatgatgct tcaactgagg gtggttatgt gatcggtgat gttcaaggaa agacatgtat
     7681 catggtagat gatatcttga acacaggagc aacgcttgca cgagcagcaa atgtattaaa
     7741 agaaaacggc gcaaaagaag tatatgcctg tgcgtcacat ggattattgt ccgatcatgc
     7801 gaaaagtatt ttagacaatg caccaatcaa agatatttgt atcactgatt ctgttttcac
     7861 agaagaaaat cgtcatccag aaaacttaac aatcgttaca tgttcagaat taatgggaga
     7921 agcggtcaaa cgcatccatg aaaatacacc gatgagtccg ttattccgtc tggaagaaaa
     7981 agaattcgaa taaacaaaaa aaagagggaa agaagttgtg acacaagtga gcagcttcaa
     8041 ggagctgcga caagactttt gtcatagctc cttttttcta tgcccatgaa acgtcctcta
     8101 gacaaaaatg agctcttctt tggtttgttc gctattttat gctagaatgt gcctagcaat
     8161 aatttggaag ggatgaatga agtgaaaact atttatctgg atcatgcggc aactaccccc
     8221 atgcatccac aagtaattga agcgatgacg gcaatcatgc aagacacgtt tggaaatcct
     8281 tcaagcatac atggatttgg tcgccacgcg catgaaaaat tggaaacagc aagacaaatc
     8341 attgcagaca gtctgcaagc aaaacctcac gagatcatct ttaacagtgg gggaacagaa
     8401 ggggataata cagctatctt agaaacagcg ttctcgtgtc aaaaagaagg cagacatcta
     8461 atcactacag ctatcgaaca tcctgctgtt cttaatacca tgaaatattt agagacaaag
     8521 gggtttgacg ttacatatct gcctgtagat gaaaaaggga atctgtcgat cgaagaggtc
     8581 aaaaaagcgt tacgagaaga tacgatcttg gtctctgtga tgtatgggaa caatgagatc
     8641 ggaaatttga tgccaatcaa agaaatcggt gaacttttag cggatcatcc agcttatttc
     8701 catacagatg cggtacaagc gtacggaaac caaacgatcc atccagaaga attaggaatc
     8761 gatttattaa gtatctctgc tcataaaatc aatgggccaa aaggcgtcgg tttcttatat
     8821 aaacgagacg gagtaaatat ccctgcattg cttttaggcg gggagcagga agaaaagcgg
     8881 cgtgcaggta cagaaaattt agcagggatc tggggtatgg caaaagcagt tgaactgctg
     8941 tcacctgaag aacgtgaagc acgtaacaac aaatataaag gattccagca attgattctt
     9001 gatacattag aagcaaacga aatcccttat caagtaaatg gtgatttaac gaataaactc
     9061 cctcatgtgt tgaatcttcg cttaattggc gaagcaaatg atttgctact gatgaagctt
     9121 gatcttaatg gggtcgctat ttctactggt tcagcttgta cagcaggtaa tatcgagcct
     9181 tcacacgtat tagaagcaat gtatggagaa gatacaccga tactaaaaga atccatccgt
     9241 atcagctttg gtttgggaaa taccgaagac gacatccagt atttccttga acaattagtt
     9301 cgtgctgtca aaaaataact agtgaagtca ttcttatatg aacagatgtc tgagagaacg
     9361 ccttcatttt ttagggaact taactgaaaa acctagtaaa aatcaggata aacttttata
     9421 ataaagagaa actaatttta tcgaggtgaa aatcatggca tttgaaacaa cagcaacggt
     9481 actgggcgca ccaattagct atcgtttgca tcctgatgca aaaagatata cattgcgcga
     9541 taatggattt acagaaacaa acggaggcaa ttatcaattg atccgaccat tagatgccac
     9601 gccgcaaagc aaagaaggat tcaaattaaa aatcactgta tcaaaagata ttcaaacatt
     9661 gaaaatgtcg atcactacag caaatggatt gaaagcagtc aatattttca aagatccaaa
     9721 acagaaaatg agccaagata aattttattt tctgatggat gggatgatta gccgtgggtt
     9781 attcgaaaaa gtcgaatgat ctgtggatat aaaaagacaa ctagttttga aacggcctat
     9841 tgttagatga ctaactttgt aaggacgacg caaattagtt gtctttttta tttttaaatt
     9901 taacacatgt tgttattttt aatattttgc tgatttattg tttctaagta aagaaattta
     9961 aattgaaacg attgttattt agggaaaaag gtattttttg aaaaaagaat cccgtataat
    10021 gattgttgtg caaacgctat acaaaatgaa aaagaaaaga gggatgttta tggaaaatga
    10081 acagtcggtc gttttaacga attggaagag aaattatttg ttctttttat cgggacagtt
    10141 tttgtcagga atcactagta tggtcgtcca atatgcgatc atctggtatt tgacaagaga
    10201 aaccggttcg gcgacaattt tgagttttgc tactttacta ggaatgattc ccatggtttt
    10261 actaagccca tttgtagaac cgctagtgga tcgctgggat aaaaaagctc ttttgattgt
    10321 cacggatatt attgtagcga ttttcgcgct tattttagca gtagtcggaa cgatttcaga
    10381 atccttcccg atttggctag tttttgtgtc tttgtttatg cgttcggtcg cacaaacatt
    10441 ccaaatgcca acgatcagtc gattatgccg acaatcgttc catcttctca cataacaagg
    10501 acgaatggac agttaggaat ggtccaatca gcgaatttca tcattgcgcc agctctaggt
    10561 gcggctttgt tttcagtcgt tcctgtcaat taccttattt tattagatgt attgggggca
    10621 gtttttggtg tcggcttatt gatttttgtg aagatcccaa aagtttctcc tgaaatattg
    10681 gaagttccgc ttactatttt taaagatgca aaatttgggc tgcagcaatt gatggataac
    10741 aaagggttat ggtacataac gatcaatggt gcatttgtga tgctgttgtt catgccagca
    10801 atcagtctgt atccattgat gacgctagat tactttggtg gttccgttgg acaggcggga
    10861 gcagtggaag tagtctacgc agtcggaatg ctcttaggcg gtgcactgat cagttttata
    10921 ggaacatgga aagaccgaat gaaacctatt attatagcct acatcattat gggcctgacg
    10981 atcggtgcaa gcggattggt tccaaacgat agtcaagggt tcttgtactt tcttatcttg
    11041 aatgcaggag caggttgtgc aacgccatat ttcaatacac tgctgatggc aatgatccag
    11101 cagagttatg aatcgaatgt tttaggtcgt gtcttaggga acttcaactc tttgatgaac
    11161 ctggcaggtc caattggatt attatttgca ggaccattag ctgatcgttt aggggtagaa
    11221 aaaatgttct tgttttctgg aatcgggatt ttattatgcg gaatcgtttt atttttaacg
    11281 tcagctgctc gaaattatga taaagaatta cagaaaaaat tggtaaaaga acaccatgag
    11341 taaaaagacg aatagtatgg aatggagttg tgacagaagt cttgttacaa ctccatttct
    11401 gatcctaatg ttcctgtggc aataagccta ctcaaatgaa aaatatgagt aattatcttt
    11461 atttgtttaa tacttgatat agggcagttt tttcacgacc tatcgtactt cctctttgct
    11521 tcatagattt ctttgatttg ttgctcagat aacggagtgc caaatggtag tttttccttt
    11581 aagagttccc tttccggtgc gtctacgcct acattgacat cgttctcgat aggtacgctg
    11641 taatgatgga tatgtccatg aagattgcgg atagtgtcgt taggcccgag caacatcgga
    11701 taatgagtca agtaatattg ttgatggtcg aatttaacga tggcacctac atcataaaaa
    11761 taaaatttgg gacttccgtc agtaagccta ggatcatttt tttctaaata acggaaaaaa
    11821 gcgcgactgt catggttccc tttgatcaag tggattgttc cttgcagttg agtgaccagt
    11881 tccaaaattt ctgcatttgc tttttcatat tgcggatgca tagcaaggtc gcctagatga
    11941 taaacatggt cggtttcttt tattactgca ttccagctag tgatcaactg ttggttcatt
    12001 tcatccacat ttgcaaagag ccgcggagca aagtcgtttt ttcctaataa gcgttcatgg
    12061 aaaaagtgca tatcactggt aaagtaattc atgtatcttt tgcctccctt ttttcttaga
    12121 gtaaacaaaa tcacttatct gagcaactca taacatctaa aaagaaaagg ttgtaaatga
    12181 gacgtgtagc tttaagcgat aagaagcaat tttgaaaaat agtttttcac aatttataaa
    12241 attattcctt attgccgaaa ggctggccct ttttatgagt atgccaattt tttatatcga
    12301 atgctcaaaa gatgagtgat tttttggttg actatcatgt ttgtgtactg aataggtcca
    12361 gtagaacagt atttttacga cttgataatt aagggatgaa attttcagaa atatgacgta
    12421 taatgtaaaa tactgaaaag ttgcgacctt caactcgtag atgtttcaga atctaattga
    12481 aatgatggtg atatcatgac agacaacagc aaaacacgcg ttgtcgtagg gatgagcggc
    12541 ggtgtcgact catctgtaac agcacttttg ttaaaggaac aaggatacga cgtgatcgga
    12601 atattcatga aaaattggga tgacacggac gaaaacggtg tatgcacggc cacggaagat
    12661 tataaggatg tagcgaaagt agctgctcaa atcgggattc cttattattc tgtgaatttt
    12721 gaaaaagaat attgggatcg tgtatttgaa tatttcctag ccgaataccg cgcaggacgt
    12781 acacctaacc cagacgtgat gtgcaacaaa gaaatcaaat tcaaagcatt cttggattat
    12841 gcaatggatc ttggcgccga ttatgtagct actggacatt atgcgcaagt cactcgcgat
    12901 gaaaacggcg tggtccacat gcttcgtgga gtagacaata ataaagatca aacttacttc
    12961 cttagccaat tatcacaaga acagctttca aaaacgatgt tccctctagg cggcatggaa
    13021 aaatcagaag tacgggcaat tgctgaacgc gcaggccttg cgactgctaa gaaaaaagat
    13081 tcaacgggaa tctgttttat tggtgaaaag aacttcaaac aatttttgag caactatctg
    13141 ccagcaaaaa aaggaaatat ggtcactctt gatggtgaag tcaaagggca acatgctggc
    13201 ttgatgtatt atacgattgg tcaacgtcaa ggactaggga tcggtggcgg aggagattcg
    13261 caagaaccct ggtttgttgt aggaaaagat ctagcgacaa acacattata tgtggggcaa
    13321 gggttccatc atcctgcatt gtatgccaca agcttggatg caagtgaaat ccactttacg
    13381 acgaatgaac caatgccaaa agaattcaag tgtacagcga aattccgtta tcgtcagcaa
    13441 gacgttcctg tgacagttcg cttattggat gataatcatg cagaagtcgt attcgacgaa
    13501 cctgtacgtg caatcactcc tggacaagca gttgtatttt atgatgaaat ggaatgtctg
    13561 ggtggtggtt tgatcgacca cgcatatcaa gaaacaaaag ttttacaata cgtttaagcg
    13621 atattaaatg aatcaaaaca gctatggcta acaaaagaaa caccttttct tttggatagc
    13681 atagctgttt ttttgcaata aaattaattt ttattcattt tatacttgaa atacttgtgt
    13741 aaaaagttca taatatccaa taatatgaat aaagttgctt tttgttaagc aagagcagtt
    13801 gcaaaataga aggaaatctt ttgcaagtga tttgttcaat tttataagtg agggtgatgg
    13861 aaatggaaaa gaaatcaacg ggtattagca aatggggact ggttgctctg gttgtcagtt
    13921 cgtcaatcgg aagcggtgtg tttgggatca caagtgatct ggctagttct gcagctccag
    13981 ggccagcgat cctttcatgg ataatcgttg ggattggtat tttagctttg gtattatcgc
    14041 tgaatcattt aggagaaaaa cgtccagatc tagatggtgg tattttcggt tacgcggaag
    14101 cttcatttgg taaattaggt ggcttcatca gtggttgggg ttactggttg tctgcgtggc
    14161 tgggaaatgt tgcttttgca acgatgctga tgagcgccat gggagaattt ttcgcgatat
    14221 tcaaaggtgg acaaaatgtt ccttctattt tatttgcaag tatttttatt tggggtctga
    14281 ctttattagt aaacaatgga gttgaaggag cgagcgtcat caatacaatc gtgacgatct
    14341 gtaaattagt gccattattc ctatttttaa tatttgtatt tatcgcattt aaagtgaata
    14401 tattcactgc tgatttttgg ggtaacgtat ctgataatct agtgaatgga acagggcagc
    14461 aaggaacgat ctggttgcag attaaaggtt gtttgatggt tatgatgtgg gtctttgtag
    14521 ggatcgaagg cgcaagtgtt ttagccaacc gcgcgaagaa acgttcagaa gcccagcaag
    14581 catcgatcat tggtctgctt tgtttattgt ttatctatat ccttgcttcg atcttgccat
    14641 acggcgtctt atctcaagaa gagttagcaa ctatttcaca acctgctatg gcaaatattt
    14701 taaaaggaat cgtgggaaat tggggagcag cgctgatcaa tatcggcttg attatttcga
    14761 tcattggttc ttggttgtct tggacgatgc tcccagcaga aacaacgatg ttgatggctc
    14821 gagacggtct gttgccgaaa ggttggggaa aaacgaataa aaaaaatgcc ccaacttatt
    14881 cgttagttgt tactgcaatc ttgactaatc tattcttatt gactttctta gttactgatt
    14941 atgcctatca atttgcctat tctttatgta cagcggcaat tttgatttgt tatctacttg
    15001 tcgggatcta tcaagtccag ttttctttaa aagaaggaat caaaaaacaa gtagtgatcg
    15061 gtgggattgc tgtagctttt gaattgatgg gcatcatttt agcaggattt tcttatgtcc
    15121 ttttatgtag tattgcctat cttcctggtt tttgcttcta ctggatcgct tgtcgtgaaa
    15181 ccaaacatca aattacggga aaagaaaaag taatgattgg gctgatttgt gtggccgcac
    15241 tgctttcaat cgggttgctt gctgtaggct ggatcaagat ataaggggga aagaaacatg
    15301 aaaatcgcaa atttcggtgt agaagaatgg ctaaataaat gggaaaagaa agcggtatat
    15361 gatattgcac aaagctcgat cgaagcattg acattagacg aactcgttgg cttagatggg
    15421 actagcgtaa gtgaattttt cgaaaaacgc aaaaacgaac cattggacta tggctggatc
    15481 gaaggatcag atgttttcaa acaagaagtc gcttctcttt atcaaaccgt cgatccggaa
    15541 aacatcttac aaacgaatgg agcgactggt gctaatcttt tagctctata cgcgttagtg
    15601 gaagcaggag accatgttgt ttcgatgctt cctacttatc agcaattata tgacatcccc
    15661 aaatctttag gtgccgctgt tgactttgtt catttgaagg aagaagaaga ttggcagttt
    15721 gatttagaac agctagaagc cttagtcaaa cctgaaacaa aaatcatctg tttgaatagt
    15781 gcaaacaatc ctacaggcac attgctagat cggaaaactt tagaaaaaat cgcagaaatt
    15841 gctcgtacag tagatgcata tgtattgata gatgaagtat atgcgccttt aacggataaa
    15901 ggtgaattct tgtcgatcgt agatgtctat gaaaaaggta tagcaaccaa ttccctatcc
    15961 aaaacctact ccattccagg tattcgtatt ggctggacag ctactggacc tgagttggca
    16021 gaagtttttc gaaagtatcg agactacacg atgatttgcg gcggagtcct gtcagatgat
    16081 cttgctgtac atgcattgaa gaataaagaa aaaatattgg agcgaaacaa aaagatcatt
    16141 acagagaacc ttgtgatttt aaaacaatgg gtagccaatg aaccgaaagt tgaacttgtc
    16201 gctcccaatt acgtctctac ttctttcatt aaattagcca tcgaagaaga tgaccagaca
    16261 ttttgtatca atttattaga agaaacagga gtactattag taccaggaag tgcctttgat
    16321 ttaccgaagc atgcccgact aggttactgt tgtaaaaaag aaaccttgga aaagggatta
    16381 ttcctcttat ctgagttctt gaaaaaacag gcctaaacaa aaacactgca aggacagtct
    16441 gtgacaactg ttcaagcagt gtttttcatt tacgcttatt gtctatgaga aggagagtgc
    16501 cctgaagata tggagactcc tgaagctttt tcttctgcag tttcttgctg atggatctct
    16561 tgtgtattgt gtcgtacaag caagaagttg accaaaacaa gaagagaagt cgaaagaaag
    16621 acgccacgat aaccaaatgc actagaaaca ctggagccga tcataggtcc tactacgtta
    16681 ccagttgctt ggaaagattg gttataactg aagatccgtc cagcagcttg atgaggagaa
    16741 tattttgtga tcaatgcttg gacagcaggg agcagacaag catcagagat acccatcaaa
    16801 aaacgtaagg cagctaattg ccaaacattc gtgacgaatg ccattgggat ataaaccaga
    16861 atcgcaaaac ccaaaccaat cgcaagtatt ctttcactcc caatcttgtc acctaagcga
    16921 ccaaatcttg gagcggctat caaagtggca atcccaggta tcgaagcgat gattccgctg
    16981 atcaacgtaa cattcccatg tccatgcagc aattggcgaa tgtacagact gatgattgga
    17041 ctaatggaat tgttggatgc ttggatgatc atcgttgtga cgaacatacc gatgacgacg
    17101 tgtggatatt tcaagtcttt gaaaatctgt ttggcagaaa ccatgttttc ttttttcact
    17161 gggacaaatt tttcatggac gaaaaacaga ctcaacaaga agacgaaaaa taagatgatc
    17221 cctgtaatga aaaaggtagg gcgatagcca aacgctgaag cagagatccc gccaagaagc
    17281 ggtccaagta atgttcctgt aacagaacca gttgctaaag ttccaagaac ttgaccactt
    17341 ttttcttttg gcgtcccagt tgcgacaagg gcagcggcat tactgatata accagaaaaa
    17401 atcccttgaa gcagacgcaa tgcgactaat tgatacacac tagtcacgca acccatcaaa
    17461 gaaatgacga ttgccattcc taatgaagca cgcaacagca tcaattttct tcctttttgg
    17521 tgggctaatt ttccccacca aggagagatc agcgtagtca ccaagaaagt agatgaaaaa
    17581 gtcaatccgc tccaaaagtt caattgtgac gtactataat tacctaacgt atctatgtaa
    17641 agtgacataa aaggcatgac taaactaaat ccgataccag ccataaatgt tccaaaccat
    17701 aagacaaaca agttttgttc ccaagtttct cttttcctaa ataatttttc ctttattctt
    17761 gcagccaatt agactccttc tttctgaaaa ttttcatcta cagctttttg ttataaacta
    17821 caatatgtag tttatcaatg aaagtctctt ttttctcttt ctttttcgtg gtaaactgaa
    17881 agagtgaagg atttatttta tgagaggaaa catgaaaatg aaaaaaagaa atctgtctca
    17941 agagaaaatc atcgactgct ttcgagaact agcagaagag atggatgttc agcaaataac
    18001 ctttcagcat ttagcaaaac gcttggatat caaggctccc tcgctgtata accatttcaa
    18061 aaatattcgt gatgtcaaaa cagcgctaac agcgaaactt ctgcaggaac ttaatgacca
    18121 gctgcgtcgg gctcttgtgg gaaaaagtgg tcaggaagca ctgcagatct atgcagaaac
    18181 ttatcgatcc tttgcttttt caaatcaagc agtgtatgag ttactgatca gtgtgcccca
    18241 caccaatgaa gcagttttat tagagggaat ccatgaaacg aaccagatca ttttgcagat
    18301 ctttgaggct tttcaattaa cgaaagaaga aaaagtccac cgaagcagag aattatgtag
    18361 tatgatccac ggctatcttt ccttgcgttt tttaggatac tttacaaaag aatcgacaat
    18421 cgatccagag gacagctatt tttggatgat caatgacttt atcgctactt tgccttaaac
    18481 aatcagattc atttatatca ttttcaagat gttcattaaa tgatcctttt ataagagaaa
    18541 attcttgcaa ctttcttgaa aagtgactaa gataagtcta gaaaagctga aagaaggagc
    18601 acgagtatgt atcaagtaat taccatgttc ggggacaatg agccttggtg gttttttgaa
    18661 gactgggaag aagatattga agcagaagaa acattcgagt ctttcgaaga agcacgacaa
    18721 gcgtacgaaa aacaatggaa cgaaatccat caaaattatg aatatatcaa tgcaaaaagt
    18781 aactttctca gcgccttttg gaatgacgga gatgagcgct ggtgtgaaga gtgtgacgat
    18841 gatttacagc aatacaaagg acttgcgttg ttgaaaaatc atcagcccat cacagtagaa
    18901 agcggaaagg aattttatga aacaactaat tctagcggaa aaaccaagcg ttgcgaaaga
    18961 cctaagcaag gtgcttggtg ctaatcaaaa gcacaaaaat tattatgaag gacccaaggt
    19021 gatcgttact tgggcccttg gtcatttatt aggattgaaa atgccggaag atctgaataa
    19081 ggaatggcag agctggcaaa tggacacgtt gccaatgatc cctaaaaaac taggaatcaa
    19141 accacttcct aaaacaggac atcaattaaa agcaatcaag cagctggctc agcgaaaaga
    19201 tgtctcagaa gcagtcattg cgacagatgc cggaagagaa ggggaacttg ttgctcgctg
    19261 gatcttagag tatgtgcatt tcaataaacc agtgaagcgg ttatggatct cttctcaaac
    19321 agataaagca atcaaaactg gattcaagca actaagacct gcgaaagaat atgatgcatt
    19381 gtatgaatct gctcttgccc gggcaaaagc ggactggctg gtaggattga atgtgaccag
    19441 agccttaact gtgaagtacg cggataattt atcagcaggc cgtgtgcaga caccgacact
    19501 agcattagtg agagaccaag aaaagaaaat cgagcagttc cgtccacaaa cgtatttcac
    19561 gatcacgttg caagctggaa aagaacaagc caaaatgctt caaaaaaatc aatttgcttt
    19621 aaaaacacaa gaagaagcac aaaaacttgt tcaattgatc agtacaagca aaggaactgt
    19681 cacagaagtc caagaaaaat taaaaacgga atctgcgcca ttgccttatg atttaacaga
    19741 gatccaaaga gaagccaatc aacgctatgg ctattctgca aagaaaacat taagtcttgt
    19801 acaaagtttg tatgagaccc ataagatcgt tacttatccg cgtacagaca gcaaatatct
    19861 gacaaatgac atgaaaagta cgatgaaaga acgattgcaa gcagtcagcg actttgctcc
    19921 tgaggtcaag cagtatttga aaaacggagc catcgtccaa cagacaaaag tattccaaga
    19981 cagtaaagtg accgatcacc atgcactttt gccaactgaa aacagagcac gttatgaaaa
    20041 attaagtaat gaagagcaaa aaatctatca aatgattgtc tctcgttttt taggactgtt
    20101 tgctcaaccc cataaagtca gccaaacaaa agttacggtt gaatttggta aagaacgatt
    20161 catttttcgg caaaatcgtg tcgtccaagc aggatggaaa ggcgaaactg aaactgagac
    20221 ggaaacagtc aaatgggaaa aagaaatgag aatcaatccc gatttcacca tcaaaaaaga
    20281 actatctgca ccgcctaaac cattgtcaga agcaagcttg cttggtcaaa tggaaaaata
    20341 cagtctggga accccagcta cgcgtgcaga gattatcgag aaactaatca aatctgaact
    20401 gatggaacgt acccctcaag gactgcaagt atctcctaaa ggtaagcaat tattagtatt
    20461 ggtaaatccc tctttagtaa caccagattt gacagaaagc tgggaaaaag accttgaagc
    20521 tattgctgca ggaaagaaac aagcaggaac cttcttgaat ggaatcgaaa aagaaacaaa
    20581 acgcttggtg aatgagatca aatcaagcaa acaagaatat caagatttct cgattaccca
    20641 aaagaaatgc ccagaatgtg gggccaatct gcgcgagaaa aacacacgtg acggcaaaat
    20701 ctatgtctgc accaattcag attgcaacta ccgcagaaga aaagatccaa aagtctctaa
    20761 tcatcgatgc ccgcaatgtc accggaaaat ggaaatcatc gaaggtaaaa acggtgctta
    20821 tttccgttgt aaatacgatg gaacgacaga aaaaatgctt gataaaaaag agcagaagaa
    20881 gaaaatgacc aaacatgaag aacgacgctt gctgaaaaaa tatgcacaag cagaagaacc
    20941 agaagaaagc ccattagcag cagcactaaa agcagcaatg aagaagtaat aagatatgaa
    21001 agaggttgtg atagaagtgg tcagcttcaa gaaataagaa ggaatttacg aaaattgctt
    21061 ctcaaatttt tgtgaaattt cggcttattt ccgaagaagc tacttctgga acaccattta
    21121 ttcggaaaaa aggactgtga caagactttt gtcacagtcc tttttcattt aaaaaaatgt
    21181 ttacttcatt tgacgtatat tattggtgag gcttcaattc atttggcttc actttttctg
    21241 tttctcttat gatataaaca ggacgctgtt tcgtttctaa gaagattttc ccgatatatt
    21301 tcccgatgat tccaagacat aacaattgga ttccaccaat gaacaggaag atcgagacca
    21361 tggaaggcca tcctgatgta ggatcaccaa acaacaatgc ccgcaaaata atgaacaaaa
    21421 ttgcaatacc agaaccgaca caagacaaag cgccgataaa tgaagcaatc gttaacggtg
    21481 catccgagaa gttcacgata ccatcaatcg agtagttgaa taatttccag aaggaccaag
    21541 atgtttcgcc agccacacgt tcgcggtttt cataagaaat atattctgtt ttaaatccga
    21601 cccagctaaa taatcctttt gaaaatcggt tgtattctgt taattctagg acggcatcca
    21661 ccatttgacg agtcatcaag cggaaatcac gagcgccatc gaccatttct gtatcgctga
    21721 ttttattgat caactggtag aattttttcg caaagaaact gcgtatcgca ggctcgcctt
    21781 cacgagtcga acgccgggtg ccgacactat cgacatcaga agtagtgatc cggttgatca
    21841 tctcaggaag cagttcgggc ggatcttgca gatcaacatc catcacggtc actaaatctc
    21901 ctgtagaagc ttgcaatcca gcgtacagag ccgcttcttt cccaaaattt ctggaaaatg
    21961 agacaaagcg gacataagga taagctgagg ctagcttctt gattgctgct aacgtaccgt
    22021 cttttgatcc gtcgtcaata aacaaatatt cgaactcatg ggcacctttg aatttttcaa
    22081 cctcatcaaa aaaaagagga acagtttttt cttcattaaa acatgggaca acaattgata
    22141 ctttcatcgt ttctatcctt tcaatcgtat gtttatatat ggtttacatt caaaatttaa
    22201 taaaccttaa ataacacctt ttaaatttga ttttatggaa aagaacagct attctttggt
    22261 aaactaacgt atttagttta ccatgtttcc tcggtttatc aaacttttaa gaacaatttt
    22321 tattagttgg caagtagttt tggatagaga aaaaggagga agttccagtc tactgaccag
    22381 gagcaaacaa aatgaaaata cacacaccta tcggcttttt atggttagat gcagatgaat
    22441 ccggattgac tgctgtatct tttcagccaa tttcagaaag cgaatcagcg aatcagcaaa
    22501 ttttaacgcg cacacaagaa cagtttgaag aatattttgc gggaaaaaga aaagaatttg
    22561 atgttccttt atctataaag agaggtacgc cttttcagca aaatgtctgg aaagctttgt
    22621 cgaccattcc ttatggagaa atccgaacgt ataaagaagt agctgttgct gttgatagtc
    22681 cgaaagctgt gcgagcgatt gggcaggcaa atcgaaccaa tccattgcca atcgttattc
    22741 cttgtcatcg agtgatcggg caaaatggaa aactgacagg atatatggga aacgcaatag
    22801 atggggttga aatcaaacgc caattgttgt tgttagaagg atatctgcaa aattaaaaac
    22861 agttgtgatt cggacattgt tccatgcgag aatcacaact gttttttcat gttcattcta
    22921 aactttgatc agccaaataa atagtaaagc catcactgtt tggagaagcc cgacagctaa
    22981 tccataagat aagaagcgtg taccttcttt catgaatttt gcaaagtcta aacgcagacc
    23041 aattgccgct aaagcagtga tctcaaacca gctgctgata aaatgtgccg attggtcaat
    23101 aactacaggt aaagaaaaca agctgttgac caaacaaaag atcacaaacc ctaatacata
    23161 ccaaggaagt ggtaattttc gttttttctg cgtatcagtt ggcgtttcaa acagagctgg
    23221 ttgtttcttg tgcatgaatt tttcaaacag atagacaaca acaacgagca tcataatacg
    23281 tagaatctta aatagcatgg cgaattcgac aacagaattg tcaagtaagc ttgccccagc
    23341 gactacttga ccaacagatt ggagtgtgcc acccaaaagt gcactttttt caatgagatt
    23401 tccaccaaac aactgtaatc caaggaaagg gagagtcaac atcatcacag tacccagtag
    23461 attgactagt gtgatgatct gtcctttttc ttcctctttc gcatggatgg aaggggcgat
    23521 cgaagcaata gcagaagaac cacaaacagc gtttcctcca gccatcatca aagaagaagt
    23581 atcgctgaat tgcagttttt taccaatcaa gtaagcaccg atgatagtga ctgacatttg
    23641 cagtaacaca aaaacaattc cttttatacc aatttgagca atcgtttgag ctgtgacagt
    23701 tgcacctagt aaaaaaacag agaattctaa tagtttgctt tcagcaaatt ttgtgccgat
    23761 tgctagaaca ggctgtttaa aaaaagtatt tcctaataaa atacccaata aaatagcaat
    23821 agtcgctcca cctaactgtg gcagataaat agcaagaagc ctgctgaagg cagcgatggc
    23881 aaaagaaagc agtaaaccgg gtaagatttc gcggaccgct gaatccttta cagattttat
    23941 tggatgtgac aatgatttca tttgatcggt tcctttcgtc aataaatact aaaagcatac
    24001 tcgaacatag tagcactaaa ccagtatttt gacaaaagaa caagaggtcg tgacaaaagt
    24061 cttgtcacga cctcttgttt cgaataaacg gtgttcaaaa gccagtttc
//
LOCUS       DACDYJ010000042        22650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDYJ010000042 DACDYJ010000000
VERSION     DACDYJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.042"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(312..611)
                     /locus_tag="IWM51_001708"
     CDS             complement(312..611)
                     /locus_tag="IWM51_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971131.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(617..847)
                     /locus_tag="IWM51_001709"
     CDS             complement(617..847)
                     /locus_tag="IWM51_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971132.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(1097..1297)
                     /locus_tag="IWM51_001710"
     CDS             complement(1097..1297)
                     /locus_tag="IWM51_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9971133.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(1602..2834)
                     /locus_tag="IWM51_001711"
     CDS             complement(1602..2834)
                     /locus_tag="IWM51_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9971134.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            3089..3658
                     /locus_tag="IWM51_001712"
     CDS             3089..3658
                     /locus_tag="IWM51_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9971135.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3836..4276)
                     /locus_tag="IWM51_001713"
     CDS             complement(3836..4276)
                     /locus_tag="IWM51_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP9971136.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            4434..5198
                     /gene="map"
                     /locus_tag="IWM51_001714"
     CDS             4434..5198
                     /gene="map"
                     /locus_tag="IWM51_001714"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP9971137.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            5230..6153
                     /locus_tag="IWM51_001715"
     CDS             5230..6153
                     /locus_tag="IWM51_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP9971138.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            6229..7368
                     /locus_tag="IWM51_001716"
     CDS             6229..7368
                     /locus_tag="IWM51_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP9971139.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            7361..8161
                     /locus_tag="IWM51_001717"
     CDS             7361..8161
                     /locus_tag="IWM51_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9971140.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            8161..8988
                     /locus_tag="IWM51_001718"
     CDS             8161..8988
                     /locus_tag="IWM51_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9971141.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8966..9700
                     /locus_tag="IWM51_001719"
     CDS             8966..9700
                     /locus_tag="IWM51_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9971142.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            9800..10666
                     /gene="rfbA"
                     /locus_tag="IWM51_001720"
     CDS             9800..10666
                     /gene="rfbA"
                     /locus_tag="IWM51_001720"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP9971143.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            10680..11252
                     /gene="rfbC"
                     /locus_tag="IWM51_001721"
     CDS             10680..11252
                     /gene="rfbC"
                     /locus_tag="IWM51_001721"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP9971144.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            11274..12302
                     /gene="rfbB"
                     /locus_tag="IWM51_001722"
     CDS             11274..12302
                     /gene="rfbB"
                     /locus_tag="IWM51_001722"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP9971145.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            12400..13251
                     /gene="rfbD"
                     /locus_tag="IWM51_001723"
     CDS             12400..13251
                     /gene="rfbD"
                     /locus_tag="IWM51_001723"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP9971146.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            13285..15318
                     /locus_tag="IWM51_001724"
     CDS             13285..15318
                     /locus_tag="IWM51_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971147.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     VGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            15362..16642
                     /locus_tag="IWM51_001725"
     CDS             15362..16642
                     /locus_tag="IWM51_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP9971148.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16852..17658
                     /locus_tag="IWM51_001726"
     CDS             16852..17658
                     /locus_tag="IWM51_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9971149.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            17670..18890
                     /locus_tag="IWM51_001727"
     CDS             17670..18890
                     /locus_tag="IWM51_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9971150.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18880..20466
                     /locus_tag="IWM51_001728"
     CDS             18880..20466
                     /locus_tag="IWM51_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9971151.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            20505..22643
                     /locus_tag="IWM51_001729"
     CDS             20505..22643
                     /locus_tag="IWM51_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9971152.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         7176 a         3833 c         4650 g         6991 t
ORIGIN      
        1 attgtgagat aataataaag aagagtttaa agcgttcccc aaaaaccact gccccataag
       61 tgtgttacgc tttaaactct tttatatttg aagccattaa aaagcatacc atatttttga
      121 aaaaaagtga gaaaaaaggc ttataattgg agtggtagtt aattagtgac ttatttttga
      181 ttttatagca ctgatactat aaaatataga tatcatcata ttacacaatc ttaatactaa
      241 cttaaaaaat atctcctttc ataagtatgg tgataaaatc cgttccgggc taccttttta
      301 ggtagcctac tttaatcttt gtatctttct ggatcaacga aagtatactt tatatagtca
      361 taacgccgat gatcgcttcg agcgtctagc acatcagtca cgatatcaaa caaaaaatat
      421 acgtctttct tcattctagt tttcgcagca gggattttaa agtagttctt attagaatag
      481 tagagattaa ttaataagct atcttcgatt gctaaaaaga aaacttctga atcccacacc
      541 ttataaaaat ctttgacaaa tctattcgaa ggatcaaatt taaaccataa ttgcgtctca
      601 ttaaaaagca taaccattac tccgatctat ttttaaactt agtctctacc tctaatatat
      661 atcgagtttt tattttgcct tcagagaata ctgtttcttt ttttgcagtt acaggttgtt
      721 tattttcgga aaaagctaat atagctaaaa ttgaaacatc catctggaat ttgtcttttt
      781 cgttgctttg ctcataaaaa tctgcatatt catcactaat attttttcta ataaattctt
      841 ccatcataaa gatcacctcg aaaagagtat acgaacaaac gttctttttg taaaggtgga
      901 atttattata ctaaataaat aggtgaacaa gtacttgtgc caaattatgt gccaaaaaaa
      961 atcgaattta ataaaacaca aacaaaaaga aatcctatta tgataacatt tcttataata
     1021 acaaacacaa cagaagacgt gtaatagtta gtcaggaacg tacaaataac ccctgtatcc
     1081 tttgcggtac agggggttat tttgtataag tagacattgt agtttctaaa ctgctatgtc
     1141 ctaaacgttt cgatacgcta agtatattta ctccttgata aagtaaaata gatgcatgcg
     1201 tatgtcttag tccatcaata aacagtcgaa gagaaaatag ggaaaaattg attactaaag
     1261 aaaaacttac taggtcgacg gtgtagttga ttttcatact tcaatagatt cgaattaaac
     1321 gatttcgatt ggcttatacc agtcctatcc aacgaaagat atatcgttct taactctagc
     1381 ggtataactt gaagctttac gtaaagccaa tgggaatgaa ctggagatga ctttttatta
     1441 tttttagtaa tagctgatgg gttaatactt acaactttcc ttttcaaagt accatataaa
     1501 aaaacggtgg ggttgtttag cgaaataaag gatctaagcc aatagcttag atcctttatt
     1561 ctatgcttta tattattttt tgcatgtgtg cgaaagtata ctcaagccca atcgccattt
     1621 cggaaaatcg gtactgtaga gccatcttca cggattccgt cgatatccat cttgtcagat
     1681 cctaccatga aatctacgtg ggtttgactt ctattcaatc cagcttctgc taattcttca
     1741 tcggacattt ctgtaccgcc tttcacactg aaggcataag cagaacctag tgctaagtga
     1801 ttcgatgcgt tttcatcaaa taatgtattg aaaaagatga tgccagattg agaaataggt
     1861 gaagggtcag gtacaagtgc gacttctcct aaacgacgcg caccttcatc tgtatcaagc
     1921 agttttgcta agacatcttc tccttgttcg gcagaaaaat caacaacttt tccatcttta
     1981 aaagtaaatt tcatacccga gatgatggtg ccagcataac tcagtggctt tgtacttgaa
     2041 atgtaaccat ctactcgatg actgtcaggt gctgtgaaca cttcttccgt cggcatattt
     2101 gccatgaatt tttctccacg agcattatag cttccagcgc cttcccaaag atggttttta
     2161 ggcaatccga taatgatatc tgtaccggga gcagtatagt gaagggcaga aaattgttct
     2221 cgattcaatt cttctgcttt ttttgctaat ttttcatcat gcttcttcca tgctaagaca
     2281 gggtcttctt catagacacg agtcgttttg aaaatctgat cccataatgc ctcaacttgt
     2341 ttttcttctg gcagttctgg gaagactttt gctgcccatt gtttaccagc cgcagccacg
     2401 acagtccaac tgactttatt tgcttgagtt gccttgcgta aattcattag tgctttgcca
     2461 ttcgctgatt gataagacgc tactcgatgg ctatctacac cagcaaatgc atcgggatcg
     2521 gcagaaacaa cgctgatcct gcttgctcct ttttcaagcc attcatccgc ttgatcgatt
     2581 ttagattgtg gtacattctc aatacgatcc gttgcagcat gtaaaagaaa ttctctttgg
     2641 atctggtcgt cagtccattg aacgataacc tcagcggctc ctagtttata agcttcttgt
     2701 gtgatcaatc gagcaagggg tgcctgttcc acgctgatct gtaaaacaac tgtgtgtcct
     2761 ttttctgtgg ctacgccagt ttctgcaatt aagcgtgcat atttttttag aagttcatta
     2821 aaatcagata acataataaa aattcctcct caccattttt tctggtaact attagcatga
     2881 taccatttaa ttatgattca aacaatgagg ctttaaataa aaaaaggagg ttattttttt
     2941 aaggttaagt ttcatgatat atgttactac ttgctatttc agcttgtgaa gaaaatcaaa
     3001 aactaatttt gcatatcaaa aaaaacatgt tacaatgaaa ttaatcaatg gtggaagcgt
     3061 tataaaaaaa ttaaggagat taaaaaatat ggcaagaaaa aagacgatta ctagagaaca
     3121 gattttggca gctgcttatg aagtagctgc caccgatgga tttacacgat ttactgcgag
     3181 aaacatcgcg aacaaaatga attgttccac gcaaccgatt tatcttgagt ttaaaaatat
     3241 ggatgacttg agacaagctc tttttgagca gatctatgat catttacaat atgaagtgtt
     3301 cactgttgag catacaggga atacgattat cgatcttgct tttaattata tccattttgc
     3361 aagaagagag aagaagttgt atcgttcact gtacttagaa gaatatggtg gaggaaaaga
     3421 gatgcagaat ttctcttata cttactttgt gaatacagtg aaacgcgatc cagcttatgt
     3481 gaatctttca gaagagcaga tcgaatcttt gtataatggg acatggatca tagctactgg
     3541 gatcgcagct ttgatgtctt caagcatcat ccatccaact gatgaacaga ttgaaaagat
     3601 gatccaagac agtatagatg ctatcttgaa attcaaagaa ccaatcgaaa tcgattgaat
     3661 atactcaaaa gttccacaaa tcagtaggta acaagagatg tgtcaaaata aggaaaagtt
     3721 cgtttttctt acagcgtatg catttttaac tcccgaagaa ctcttccatg aatgcaatga
     3781 ataaaagagc catacagatt gaataaatct ggatggctct tttattgcaa gtatattaaa
     3841 tttttgcgac tagttcttta gcgaaagctt ctaaattttc gatatcttct tcttcagcat
     3901 tcagatccac tttaacattt tcggctcctt ttgatgcgcc tgtttttgca aaagcagcat
     3961 cgaaatcatc caccgatttg cagaattcat cgtaaaaagt atcaccagat ccgcagacac
     4021 cgtaaatttt tcccaataga tccaattctt gcaagtcttc ataaaagtct acgatctcat
     4081 caggcagatc gccatcatcg tatgtataag tagctactac gcaaatatct gcctcctcga
     4141 attcatctgc atctacttgg gtacattcgt tgatttcgac ttcgatatcc agattttcaa
     4201 atgcttctgc aacaatatcg gcgatttctt cagtgtttcc tgtcatgcta gcatatacga
     4261 tttttgctaa agccatgatg tgcctcctcg tttataatta tttaacaatt atgaattata
     4321 ccaaaacatc attgaaaaga aaataaaacc tagagacacc tggggaatgt catttggcta
     4381 aatgtatgcc gctatgttaa aatgagaggg atttgtaaaa aggagacgaa aatatgatta
     4441 ctttaaaatc agcacgtgaa attgaaatga tggcagaatc tggagcttta ttagcagatg
     4501 tacataaaaa tctgcgtgat tttataaaac caggaatcac aagttgggat attgaggtat
     4561 ttgtcagaaa ctatatcgag agtcatggcg gaattgctgc gcaaattggc tttgaagggt
     4621 atgaatatgc gacttgtatc agtataaatg atgaaatttg tcatggattc cctcgtaaaa
     4681 agcctttgaa aaacggtgat ttggtaaaag tcgatatgtg tatcgatttg aaaggcggca
     4741 tttctgattc ttgttgggcc tatgtagttg gagaatctac acctgagatc gaccgattga
     4801 tggaagtaac gaagaaagct ttgtacatcg gtattgagca agcacaagta gggaatcgga
     4861 ttggtgacat cggtcatgct atccaaactt atgtagaagg cgaaaatcta gcgatcgttc
     4921 gtgattttat tggtcatggc gttggtccta cgattcatga agaaccagca gttcctcatt
     4981 acggagaagc tggaaaaggg ttgcggttga aagaaggcat ggttatcacg atcgaaccta
     5041 tggttaatac cggaacttgg aaaatgaaaa tggatcctaa cggctggact gcgtatacaa
     5101 gagacggcgg actttcttgc caatatgaac atacattggc tattactaaa gatggtccaa
     5161 gaattttgac atcacaagga gaagaaggca cttattgatc tatccagtca ataggtacgg
     5221 gagaggtaca tgaaagtttg gaacaaaata aaacaaaatg aaaatctgat gcgctttatt
     5281 gagacgactc aaaagcgcat cactgattca gaaatgagta cgacctctgt cgtcgttgct
     5341 tattatttac tcttgtcttt atttccattg attattgctt ttggtaatat tttaccgttt
     5401 ctccacatcg atcaagagac tgtactgacc tatttacgag aagtgattcc tgagaccgtt
     5461 taccagttta tcggtcctgc aatcaaagac cttttgacac aaagttcagg aggcttgtta
     5521 tccgtttctg cattagcggc tttatggtct gcaagtcaaa gtatcaatgc tttgcaaatt
     5581 gcgatgaata aagcgtatgg cgtagaaaat agaaaaaatt ttttgatcgt ccgtttcttc
     5641 tcactaattg tcattgtctt gttcatgatt gctattagtg gggtaactat cgttcttggg
     5701 ttgggacagc tgattttgga tgctattcag ccaatcgtac aaatacccgt gaactttatc
     5761 gatcagttcc aaactttgaa gtggccaatc acattggtag ctttatttgt catcatgttc
     5821 ttgatttatt taattgtccc taatgctcag ttgaagttga agacgatcat tccgggcacg
     5881 atcttcgcta ctgtcggctg gatgctgtta tcacaagtgt ttagtatcta tgctaggtat
     5941 tttgcaacga ggatcagcgg ttatcagatc ataggaagtt ttattgtact catgctttgg
     6001 cttaattttg ctgctacgat catcattttg ggcggaatca tcaatgcagt tgttcaagaa
     6061 tacgtgacgg gtcatcaaat caaaaaacgt cgagatccaa gccgaaaatg gttcaataaa
     6121 ttgaaaacta aattttctca taaaaaaaag tagaaacaag agttcttatt gtaaatttat
     6181 atcagtaatt tataataatt atgactctta attaaagtag gtgcccatat gcttgtttct
     6241 tttagtatgg ttataagatt ttttctgaca attttattat cactgatatt aacacctgta
     6301 ttcaaaatca tttcagtaca aacaggtatg gtcgacaaac caaatgaaag aagaataaat
     6361 aaagttccaa tgccttcagc gggcggactg cctatcttta tttcatttgt tatatcgact
     6421 ttattcttat tccgtaaaat tatccctcaa tcgtatattg taccgatctt gattgcttct
     6481 ttagtaatca tagtcacagg agtgctcgat gataaatatg agctgacacc taaacaaaaa
     6541 agtataggga tcttgattgc ttctcttatt atttattttg tagcagatat acgtattgat
     6601 tccttcacgc ttccttttat tggatatatc caactaggct ggcttagttt tcccgtaacg
     6661 attttttgga tcttcggtat cacgaatgcg gtcaatctga ttgacggttt ggatgggctg
     6721 gcggcaggga tttcattgat cggtttaatt acgataggaa tcattggata ttttttcctc
     6781 catgcttcta cagtgtatat tccagttgtt attttctgtc ttgtagcaag tattatcggt
     6841 ttttttcctt acaattttta tccggcaaag atttatttag gcgatacagg agctctattc
     6901 ctagggttta tgatggccgt cctttcacta caagggctga agaacgtgac ttttgtttca
     6961 tcgatttctt tgctgatcgt catgggagtg ccggtaacag acacgttttt tgctatcatt
     7021 aggagaaaag caaaccgtgt ttctttttct acagcagata aaaaacatct tcatcaccga
     7081 ctaatcagtt taggctttac tcataaaggt gctgtcctga cgatttatgc gatggctttg
     7141 atgttttctt tcacagctat ggtcatgaat tatactggaa gaatcggaac gatcatattg
     7201 attattgcca tgttgtttgc tgcaatttta ttatttgaat tgattggtct gatcaatgag
     7261 aaacatcagt ttattttaaa aacattgcga tttttaggga ataaagagta ccgtacgcaa
     7321 gtaaaacaga aatatttgaa gaaatggaac cgtcatatag atgaataaca aattaaacca
     7381 gccgatcctt attagtattg tgacggcaaa cagtgataaa atttttcgga cactagataa
     7441 ttttatttca actgtaaaag atgaaacagc agtaaaaatc cgaatttttg acaacaattc
     7501 acttcccgag tacaaaaaaa gactaacaga gtataggaaa tatccctaca tcgatatcta
     7561 ttttcatgat gaaaattccg gatttggtta tggacataat cacaatctga tagctagcga
     7621 ttctcgctat ggcattattt gtaacccaga tattttagtg gatgaagaga cagttacagc
     7681 attggtcttg ttattgaaag aacatccgga atgtgcgatg ttagcaccta agattcttaa
     7741 tgaagatgga acaactcagc acctcatccg tgagcagctg gctgtttttg attacgtttt
     7801 gcgctttgtg cctttcaaat gggtcaaaaa aaagtttgac aaacgactgg ctgtgtttga
     7861 atgccgagga ttgcctactg atgctaatag ttatgtacgg atgatctcag gtagttttat
     7921 ggtagctgat attgataaat ttaaagagat aaaggggttt gatgagcgtt tcttcatgta
     7981 ctttgaagac aacgacttgt gtttgagttt tgaaaaagca ggttacaagt tattgtacac
     8041 acccttttat gcagttatcc atttttacgg gaagggtgcg catcgaaatg caaaactgtt
     8101 tcgcgtgttt ttgacttcta tgatgaaatt tttcaataag tgggggtggc gttttttctg
     8161 atgaaaccag aaatattagt agtcgttgtg ctttatcagc aactattcag tcaatctcca
     8221 gcttatggac cattaagcaa agcacttagc gaaaagcaaa ttcagcttat tatttacgat
     8281 aatagtcctc aaaggcagca aaatgagctg tttgaaaaag aaaatatcgt ttatcatcat
     8341 gatccgcaaa atcctggttt agcggtggct tataattttg cgttgtctca agcagatgaa
     8401 ggaacacgtt gtctcgtcac tctggatcag gatacgaggc tagtcgacga ttattttgaa
     8461 ctattgcgta aagtaacctt tacagatgaa tgtgttgctg cagttccgat gattttttct
     8521 gggagccgac aagtttctcc cctatattca gatcgttata tcaatcgtac agctcaagca
     8581 gtagaagttg aaggcccaac agctgaacga atcatggcaa tcaattcagg tgttgcctgg
     8641 tctgtcaaat ttctaaaaaa gattggcggg tttaatcctg ctttttcttt agattttttg
     8701 gatcattggc tgttttggaa agtggatcag ttggaaaaaa ctgtggagat ccttcctgta
     8761 cgaatggaac atgatctttc ggttttagac tatgaaaaag tttcaagtaa tcgataccgt
     8821 tctattttac aagcagaaaa tcgattttat catgaatatg atcaaaggca tttatccagt
     8881 caccgacgac aattattgtt aagaacagct aaacagttct tgaccgtcaa aaaccggcat
     8941 atctggcgga tgactttgcg ctcatatgtt aagaattgga aggtgtgaat tcgtgcgttc
     9001 ggtttgtata gctaccttta atggaggcag atatttaaga gagcagttag atagtatttt
     9061 atctcagctt tcagcagaag atgaagtagt catttctgat gatgggtcag tagatgatac
     9121 atgggatata ttaactgaat acgcagaaaa agataagcga atccagcttt tttcggggcc
     9181 tcgtcaagga ttgatcgcta attttggtta tgcgatccag cagacgaaag gagatctaat
     9241 ctttttggca gaccaagatg atgtttggtt ggagacaaaa gtgaaaaaga tcactgacta
     9301 ttttgagcag aatccggatc agctagtagt agtttctgat ttaatcattg tggacagcga
     9361 gctgcaagag atccatgatt cgtatttttc gtttcgaaat tccaaagaag gatggcttaa
     9421 taatgtgata agaagtcact atattggtgc tggtatggct ttccggtctt ctttaaaacc
     9481 cgatattttg ccgatcccgt cagaagtgcc gatgcatgat atgtggattg gtttgctggg
     9541 aggaaagaag actgggttcc taagagaacc gttaactctg taccgaagac atgatttaaa
     9601 cgcgagtgag attgaaacac attctaaatt acggcaaaaa atttcttggc gtgttcatct
     9661 gattacggcg ttgatcaaac gaaaatggtt taaacgttga taaaagatgt ttaaatcctt
     9721 aatatttcca tgacaaatat aacaattgat gagataataa caaagtgaaa tttgaaatca
     9781 aaaaggagag cagatatcca tgaagggaat cattcttgct gggggcagcg gaacacgtct
     9841 gtacccttta acaaaggcaa catcgaaaca attgatgccg atttatgaca aaccaatgat
     9901 ttattatcca atgtctactc tgatgttggc gggaatcaaa gaaattctga ttatttcaac
     9961 gccacaagat acaccacgtt tcaaggaatt atttggaaac ggaaatgatt taggactgca
    10021 gatcgaatat gcagtacaag aaagtccaga tggattggca caagcgttta tcattggaga
    10081 agaatttatt ggagatgata gtgtctgctt ggtattaggg gacaatattt actatggtgg
    10141 cggtctgtct aaaatgttgc aacgagcagc ttcaaaagat acaggcgcga cagtatttgg
    10201 ctatcatgtc aatgatccag aacgctttgg tgtagtagaa tttgatgaga atatgcaagc
    10261 tttgtcgatt gaagaaaagc ctgctcagcc aaaatcaaat tacgctgtaa ctggccttta
    10321 tttttatgac aatgaggtaa tttctatcgc taaaggtatc aaaccttcag aacgtggaga
    10381 actagagatt acggatgtca ataaagcata tttggataaa ggaaaactat ccgtagaact
    10441 tatgggacgc ggatttgctt ggcttgatac aggaacacat gaatcattgc ttgaagcatc
    10501 tacctttatc gaaacaattg aaaaacgtca gaacttaaaa gtggcttgtt tggaagaaat
    10561 tgcttaccgt atgggctaca tcgataagga acaattgctg gcccttgcac agccattgaa
    10621 aaaaaatcaa tacggtcaat atttattgaa tttggccgct gaataaggag tccttacaga
    10681 tgaaagtaac agatacaaaa ttaacagatg tcaaaatcgt agaaatggac gtctttggcg
    10741 atcatcgcgg tttcttcaca gaaagttatt caaaagcaaa atttgcagaa catggattga
    10801 aatatgactt tgttcaagac aaccattcac tatcagctga agctggtgtc cttcgggggc
    10861 ttcatttcca aaaaggagaa gctgcacaga caaaattgat tcgggtagtc actggagcag
    10921 ttttagatgt agtagtggat atccgcaaag gtagcccgac ttatggtcaa tgggacggat
    10981 ttatcttatc tgaacataat catcgacagt tgcttgtacc taaaggatat gcccatggat
    11041 tcgttacttt gacaccaaat gtgaacttcc tttataagtg tgataattac tataatgcag
    11101 aagcagatgg gggcatcgca tttgatgatc cagatttagc gattgattgg ccaattgatc
    11161 catcaagagc gattatgtcg gaaaaagatc accgtcatcc gacattaaaa gaatttgaag
    11221 cagaaaatcc atttgtttac ggagaaatct aagaattaag gagaaatttt cttatgaaaa
    11281 acatcattgt taccggaggt gccggattca tcggctctaa tttcgttcat tacgtggtaa
    11341 acaatcatcc agaagtgcat gtcactgttt tggataaact gacttatgca ggaaacaaag
    11401 agaacttggc tggacttcct agcgatcgcg tagaattggt cgtaggagat gttgcagatg
    11461 cagaattagt ggaccgtcta gtaaaagaag cggacgcagt tgttcattac gcggcagaat
    11521 cccataacga taattcatta aaagatcctt tcccatttgt acaaacgaat atcattggta
    11581 cgtatacact tttagaagct tgccgcaagt atgatgtacg ctatcatcac gtatctactg
    11641 acgaggttta cggcgatctt ccattacgtg aagacctgcc aggacatggg gaaggcgaag
    11701 gagaaaaatt cactgcggaa acaccatata atccttcaag tccatattct tctacaaaag
    11761 ctggttcaga tctgttagta aaagcatggg tgcgttcttt tggcttacgt gcaaccattt
    11821 caaactgttc aaacaactat ggtccttatc agcatattga aaaatttatt cctcgccaag
    11881 taacgaatat cttaagcggt attcgtccaa aattatatgg cgaaggaaag aatgttcgtg
    11941 actggatcca tacgaatgat cattcttcag cagtgtggct gattttgacg aaaggcagaa
    12001 ttggcgaaac ctatctgatc ggtgcagacg gtgaagaaga taataagacc gtgatggaat
    12061 tgatcttaga aatgatgggt caaccaaaag atgcgtacga ccatgtaaac gaccgtgcag
    12121 gacacgactt gcgttatgca atcgattcga caaaattacg ggaagagtta ggatggaagc
    12181 ctgagtttac gaacttccgt gatgggttgg ctgacacgat caaatggtac gaagaacatg
    12241 aagactggtg gaaaaaagaa aaagaggccg ttgaagcagc atatgccaaa aatggacaat
    12301 aatacagaga aaatgtcact gtattaatgg aaaaacacat gtgaggcagg cgctcgcctg
    12361 tctctttttt aaaataaaaa tcaaggagcg gataatcata tgtttttatt aactggagga
    12421 aatgggcaac taggtacaga attgcgccat ttattagatg aaaaaaatct gcaatatgtt
    12481 tcaacagatg caaaagactt ggatatcaca gatgccgaga aaacaatggc ttacatcaca
    12541 gaattaaaac ctgatgttat tttccattgt gcagcttaca cagcagtaga taaagcagaa
    12601 gaagaagcaa aagaattaga tgagaagatc aatgttgatg gtacaagaaa tgtagcactt
    12661 gcagcaaaag ctgtgaatgc tcaattcgtc tatatcagta cagactatgt tttcgatgga
    12721 aaaaacaaaa atgtagaata cgaagtagac gaccaaacga atccactaaa tgaatatggt
    12781 cgtacgaaat tattaggcga aaaagcagta gaagagattt tagaagacta ttatatcata
    12841 cgaacttctt gggttttcgg gatctatgga cacaatttcg tcttcacgat gcaacgccta
    12901 gcggaaaccc gcgacaaact gactgtagta aatgaccagt ttgggcgacc aacttggaca
    12961 cgtactttgg cagaatttat ggtatatgtc atcgaagaaa aagcaccatt tggtatttat
    13021 catttgtcga acgaaaatag ctgttcttgg tatgaattcg caaaagaaat attgaaagat
    13081 acagatgtag aagttgcacc tgttacgtca gaagaatttc cgcaaaaagc aacacgtccg
    13141 cagtattcag tcatgagcct gaaaaagaca gaagagttag gatttgtcat accaacttgg
    13201 caagaagcac ttgcgcaaat gctggaagct gccaaaaaaa tgaataagta atttcctttt
    13261 atcaaataga atttggagca tgatatggaa aaacaaacaa gaagtgcacg acgaaaagaa
    13321 aagccaaaag cgaaccaggc agattcgaat ttggtgcttg acctgtttct aaaactccgc
    13381 tatcttattg gcgttttagt catcgtggtc atcgttactt ttaatttaaa cggaagttca
    13441 cttggtgttt gggataaata tgtgtcgcaa agagacgatg ggaaaaaaac tgatgtgatt
    13501 tttggacaga atcgggaagt gcggtcagac gaatggctgg tgcagacacc tttttacctc
    13561 tcacaagctg aagaggactt tccgttggtg aacgaggatt attcattaaa cggacaaaac
    13621 atgatcattg cgtataattc tcctgcaaag gatattacgg ttatcggaaa accattcaac
    13681 tggggattct ttttcctcgg aagagatcga ggactttcct tttattgggc gatgaaatta
    13741 gtcgtcatgg tcttgcttgg attcgaagta ctgatgatat taacgaagag aaaaaaagct
    13801 ctatcgctaa tcggtgcatt tgcgctgaca ttttctccag cagtccagtg gtggttcatg
    13861 caacatgtgg gcgatttgat tttctttact ttgggactga tgattgcttt ttatcattat
    13921 ttctacaatc atgataaaaa atggctacga acgttgatga tgcttttggt ggttattttc
    13981 ggtattgggt ttattttagt gatttaccca gcccatcaag tgatgctggc gtacttgctg
    14041 attttttatt ttgttggcct gcttatttac tatagcaaga aagtttcatg ggatctgttg
    14101 gatgtagtgt tggttggagg ggcagtttta tttattggcg gagtcatggt tcatttctgg
    14161 ctaacgtcaa aagacgcatt gttagcttct ttgaatacct tatatccagg aaaacgggta
    14221 agtactggag gaaagtggac gattggcgaa tttttttctt tccttacaaa ttggaaaatt
    14281 ccttttgaag atatcaacta ttcgaataac tctgaagtgg ccctgttcta tcactttttt
    14341 ccaactgtat tcctagcaag tccatttgtg ttatttggca aaaaagatag cgaacaaaag
    14401 ttactgggac gtattttgat gattttttgt ctgttttcta tcttttggat taccgttggg
    14461 ttgccaaaat ggctagcaga aatcactctg ctgtcttatg ttcctccatt aagagccatc
    14521 cttactttta gttttgcggc ttgtttatta tcgatctggt ttatcgctta tatttggaga
    14581 gaagatgtta ttcctatttg gtataaattc attttgttgg cagtaaatgc atggtcttat
    14641 ttctatgcga ttacgcattc gaaaatggat aattattttt cggattttga aaccatcgca
    14701 gttgctgtgt tgggaacagt gattttagct tttgcattat ttggctggag acgcttgttt
    14761 tacctcgctt ttgtcgcctt agttatcgta tctggctttt tcgtcaatcc agtcgtagaa
    14821 ggtacaggag cgatttttga aaaaacactg gcaaaagaaa tccaaaaaat cgatcaacaa
    14881 gatcctggta aagtttggtt gtctgaagat gaattatata acttcacccc aacattggga
    14941 gtgaaatcct tcaatacagt acgcttttat ccagatatag ctgcatggaa gaaaatcgat
    15001 ccgaaagaag aatacgagaa gtattataat cggtatgctc atacaagagc ttttatcgct
    15061 aaggaagata caaagttcat tttagatcgt ccagatatgt tttcggtcac gttgaatttt
    15121 gaagacgcag aaaaattagg gatcgactat gtgatcagta aacgaccatt aactgattac
    15181 aaccaattat atgaagcgca gttcacgcag ctatatggac cggataaaga cggttatatg
    15241 atttttaaag tagaatatcc ggattatcca gatgtaccac aaacacaaac tcaggaaaat
    15301 cagctagtcg tacagtaata ggctagctga gaataatcct gaaatattag tagaaaggag
    15361 agtgaagata attcaagcaa tcgtacaaaa atttgtcaac aaaatgagtg tcggtttctt
    15421 gcttttgttg ctgacgctag tcttttttgt ttggacagca ggggtaggtt ttccagcaac
    15481 tttgtttata tataacaata gttcattact tatcgctttt agtgtggcta ttttgctcat
    15541 tttaaatgtt tataaagcaa aaggcgtcga ttttctttat ctaggtgttg cgcttgccct
    15601 tttcattttc ttttcttact ttcgggaatt aagaaatgag tctttatttg gagacttact
    15661 gtttcctttg attattttat tttgtttagt aattaaatgg atcgagtgga caaaatggga
    15721 tcgcggtatt tatctgattg tctttacggt cttcctcgga atcacgttat atcgtgtatt
    15781 tacagagatc caagtaccag aaggacaaag catctgggct ccaggcaatt cgctttctga
    15841 tatttggatc aatacgaata caatcggaag ttctttgatg actcttgctt tattgatcag
    15901 tggatttgcc agttcttttg aacgatggta tgttcgaatc atcggtggga tagcagtcgt
    15961 tgcagccttt ttaggtattt gggtctgcca atctagaggt gcattagtcg cgttgattgt
    16021 ttttgcgata ttggatcttt tacctaaatc gttgatgcgt gtcattcgtg cgccatttat
    16081 tgcctacacg gttacgattc ttttagcttt gccgatttct tatctggcgg ctgtctctga
    16141 aaaagtcaat ttgtttaccg gtcgagaaga tatttggcat aaattctatc agacgctagg
    16201 agaaaaatct gaacaagttt tactaggaat gaaaacattt atcttccagc gaggaaacca
    16261 atttttagga aaccacaata gttataattc tattttgaat atctatggat tgataggatt
    16321 tgggattgct gctttgctgc ttatcctttt tattggtcgc ttaacgttga aagcagattt
    16381 atccaacggg cagatgactt ttatctgggc cttttttgcg gtgatgatgc agtcatttat
    16441 ggaggataca ttgacttcat acccatgggt tccgattatt tatatcctgt tggctatggc
    16501 cgttcatcga tatgatgaac cagaagttac ggaggaattc tatgttatgg aagaggcata
    16561 tgacgatatg acttatgatg atacatctta tgaggaacaa ccagatagcg ctcattcttc
    16621 caggatccaa cgctatcatt gataggcaca aaatacgaga gaggctgtga caaccgtcgc
    16681 agcctttttt ggtttattgc aattgtctag catcttctgt aaaatagaaa cgttagagaa
    16741 aatcttgtat catagaaaat taagaaagaa ataagaacgc agctcggtac caaaaatatg
    16801 gtaaaatggc acttgactgt aattcactga aaatcgcttg gaggaacaca aatgtttaaa
    16861 gatttgtatt tatttatgaa ggacatttat cagaacagaa aattactgct acagttttcg
    16921 atcaatgact tcaaatcaag atatgcagga tcgtttttag gaatcttatg ggcgtttatc
    16981 aatccgctat ttactgtatt gatttattgg ctggtatttg gtgttggact gaaagcacaa
    17041 atgatcgatg caaaataccc atttatcgta tatttagtta ccgggatgat tccctggttc
    17101 ttcttctcag atgcatttgc atcaactacg gttgtttttc gggaatatac ttatttagta
    17161 aagaaagtcg tattcaatat tcgtatcttg cccacgacga aaatcctatc caatctgtac
    17221 acccatttat tcttcattct gattggcata gctgtcgcgc ttataaatgg tatatatcct
    17281 tcagcaatgt ctttacagct gatttattat ttattttgca tgtgtgcttt cttgacagga
    17341 ttgacttggc tgacggcatc gatccaacca tttttacctg atatcatgca attaattacg
    17401 atcgtgctgc agttgatcat gtggacatta ccgatattat ggagccctaa tcagtttgat
    17461 tcaggtcttg taagcatatt gaaattaaat ccgctgtact atgttgttca aggttatcgt
    17521 gagtcatttt taagtgaagc ttggttctgg gagcatggaa catatacttt gtatttctgg
    17581 gttttcacgt tagtaatgtt cgtaatcggc tctgcagtgt tccgtagatt gaaaccacat
    17641 ttttcagatg tgctgtaagg agaatagcta tgacagaata tgcaattaga ctaaaagata
    17701 tcacaaaatc ttataatatg tacgcaaaac cttcggatcg atttcgtgaa gccctcaatc
    17761 cttttaagaa atcctatcat gatgtatttt atgcgttgaa aaacgtgaat gtagaaataa
    17821 aaaaaggtga gatgatcggt ttcgtgggag aaaacggatc gggaaaatct acaatgttga
    17881 aaatcattac gggagtactg acaccaacat ccggcacgat ggaaatcgaa ggaaaaatct
    17941 cagctttgct agaactagga tcaggattca atcctgagta ttctggctat gaaaatatct
    18001 atttaaatgg aatggttcta ggattttcaa gagaagaagt cgatgaaatg gtcgatgata
    18061 tcattgaatt tgctgacatc ggcgaccata tttatcaacc tgtaaaaacc tattcaagtg
    18121 ggatgttcgt tcgtctagct tttgctgtag cgatcaatgt gaatcctgat attttgattg
    18181 tggatgaagc attggcagta ggagatcttg aatttcaact aaaatgtatg gaaaaattta
    18241 cagaaatcaa gaactcggga aagacgattt tattcgtttc tcatgatatt agttcgattc
    18301 gccgtttttg tgatcgttcc tattggttga aaaatggaaa agttgtcgaa tatggtgata
    18361 cgatggaagt aacgacgaat tacgaaaact ttttgaagaa aaaatcagtt aaaacagtag
    18421 atcgaaataa aaaccaagta gaacgcttcg cagctccaga tatcgtagac gtgacgaaag
    18481 ccgaattgtt ggataaagat aaaaatccag tggatatgct ggaacaagga gaaaaactat
    18541 acgttaaagt aacctatgaa gtaaaagacg atacaatcaa aaatccagtt ttaggaattg
    18601 ctctgcgtac agtcgacaat ttttatgtgt gcggactcaa tactctacta gatggtatca
    18661 agatcccttg gaaaaaaggt gagaatatct tttatctgga gtatgaaaag ttaggtttgt
    18721 tagctggtga atattatttt gacgttgcgg tctttgaaga aaatgcaacg gttcctttgg
    18781 tatacaagac aaaatacatg aacttgtttg tcagcggttc ttatatcgga gaaggcatcg
    18841 tggtcttaga ccataaatgg gaagaaggga ctcatagcaa tgaaatatga ttttgaaatg
    18901 gacctagatg aacaaagcag tgtgggaaaa attgccgcac agatcaaacc aggaagtaaa
    18961 gttttagagt ttggtcctgg aaacggccga ttaacgaaac atttgatcgg agcaaaacaa
    19021 tgtgaagtga gcattgtcga gctagataag gaattgttcg attttgtcag cgagtttgcc
    19081 caagatggat tttatggaga tatcgaaagt tttgaatggg caaactatta tgctggacaa
    19141 acatttgatt acgttttgtt tgccgatgtt ttagaacatt tggtagatcc tggaaaaacg
    19201 ttgaagaaag ttagagaatt tttgaatgaa gaaggagaaa ttctaattac attccctaac
    19261 cttgcgcata attctgtcat gattgatttg ttcaataatc aattgccatg ggcttcttat
    19321 ggtttgctag atgaaaccca taattctttt tatactcatg atggcttcca aaaagttttt
    19381 gaaaaagcag gattatacat caatattgaa gattatttgt acttagctgt aggtgataca
    19441 gaattaaaat caacttatga agaattgccg gaagctgtac gttatgattt caaaatgcga
    19501 ccatttggcg aagtctacca atacttcttc tcattgatga aacatccagt agcacaaagt
    19561 tccattgcgc agccgcaaaa ttctaattat gtgaaagtat tagaagtaat ccagcaaacg
    19621 aaacaagatg aaataattca acaaatacca ttcaataatt tcacagggga aaatgagaca
    19681 ctaagtttcc atgtatcaga attagcagaa cgaatggtgt ttcgcttcgc gcagcaacct
    19741 agctttatcg aattcagcgc agaagctgct ggagaaaaac tcacatttat cgattcaaat
    19801 gctgtagtca aaacagtaaa tgattgttat ttgtttgatg gtaaagattt accagagttc
    19861 tttttaacgg atattgcagg gaaagaagtg actatccatt gtcactatcg ttttatcgga
    19921 gaattgacac caacaatgaa agaattgctg gaaacaatcc gtccaatggc tgaagttaca
    19981 aagcagcttt ctgagaaaaa tgatgaatta gaaagagaaa atcattatct tattgagaat
    20041 aatacaagat tagatcagac gttgaaaaca accataaatc gctattgcac gttgttggaa
    20101 agtgatgaat ggacaatcaa acatagatta gggcgacgta agaaagaaac atcgaaaaaa
    20161 atccaagaaa aagagctttc tctatgtatt gacgaaaaaa tctgggatgc agaaacaaag
    20221 atattgaaaa tcatcgggtg gggaatcgct aattcggatc gccaaccatt gtcctataag
    20281 ttatcagccg accaatcacc attttttgaa gcaattcctg ttttacgaga agaagtcaat
    20341 caagcaaaac agctagctga aggagcaaaa gcaggatttg aactgcggat cctttgcgag
    20401 aaggaacgtt cattcttagt tgaagcagtg gctcaaaatg gtcaatcatg gatcattgaa
    20461 atgtaaataa tgattaactt ataaagataa ggagtaaacg aatcgtgcaa aaaaaagaaa
    20521 ttgccgtatt tatcgatcaa atcactcgtg aacgggccac tggcgatttg cttattgtgg
    20581 gctgggcaat tgatgaagtg accaaagaaa tccctacgat aaaggtagaa aaagaaaatg
    20641 tgattgctga agcaacacat gtcgtaagat tggatatcaa tcatttatat aatcttgatg
    20701 ttaagaccca aagcggtttt aaggtacgtt tatcagggaa aatgagagga aaagcaatct
    20761 tagacttcca aacagcaaga catcaaaatg gcatagctgt gaaattaaac ggtaaatatc
    20821 cttatgatga cggcattgaa tcttcatggg aaagaaaaaa aagattactg aaaaaaggga
    20881 taaattatgc aaggactcat ggcgtaaaaa aagcaatccg ccgggtcaaa ctggaattga
    20941 aaccaggatc gatcgactat gcagagtgga tcagtcgtca tgagaaacct gatctgaaag
    21001 agcaaaggaa agaagtacaa ggatttgcct accgcccatt gatttccata cttatgcctg
    21061 tctataacgt tgaaatcaaa tggctcgaaa aatgcattga ttcggtactt gatcaaacgt
    21121 atgatcactg ggaactatgt atcagtgatg atgcttctac tgatccagct atccgaaagt
    21181 gtctagaatc ctatcaagca aaagatgata gaattaaagt cgttttccga caagaaaatg
    21241 gacacatcag tctggcaact aattctgcat tggaaatggc agaaggagag tttatagcgc
    21301 tcttagataa tgatgacgaa ttacctccat ttgccttata tgaagtggca aaagtgctaa
    21361 acgtacatcc agaactggat ttgatctaca gtgacgaaga taaaatcgat gcagatggga
    21421 atcgctttga cccgcacttc aaagcagact ggtctcccga tacattgatg ggaaataatt
    21481 atatctctca cttaggcgta tatcggacaa gcattgttaa ggaacttggc ggtttccgta
    21541 aaggatatga aggctctcaa gattatgatt tggttcttcg tgtgacagaa caaattcctg
    21601 cagaacacat ttaccacatt gaccgtgtct tgtatcattg gcggacgatc ccgggatcaa
    21661 ctgcaagcaa tggagaagct aaaagctaca tctatgattc tggagtgaaa gcattgacag
    21721 atgctctatc tagaagaaat atcaagggaa gcgtacatcc tggcagaatt tctggttttt
    21781 atgaaatcac ttatgatgtt ttgcaagaag acttggtcag tgtgatcatt ccaactaaga
    21841 atggctatga agatctaaaa acttgtgtgg attcgatcat cgaaaaaacg tcttatccaa
    21901 attatgaaat cattattgca gataatggaa gtacagatcc taaaatgcaa gaattatttg
    21961 cagaatacaa gcatcagctg aaagaccgat ttatcgtcga attaattgac attccattca
    22021 attattcacg tatcaacaat ctggcagcag aaaaagcaaa cggcaaatac tttttattct
    22081 tgaataacga tacagaagtg attgaacctg actggatgac agcgatggtt tcttatgcac
    22141 agtttgatcg aatcggctgt gtaggagcaa aattatttta tccggatgat acaacgcagc
    22201 atgcaggtgt attattaggg atcggtggcg ttgcaggaca tgcattgaat aactatgatc
    22261 gaacacattg tggttacttt ggccgattgg tcattgacgt caattactta gcagtcactg
    22321 cagcttgtat gatggtcaag gcagctgact tcaatgctgt taacgggttt gatgaaacat
    22381 tagaagtagc attcaacgat gttgacttgt gcttgaaagt ttatgaactg ggtcgttaca
    22441 atgtctatgc acatcaagca gaattgtacc atttcgaatc taaatcaaga ggttatgaag
    22501 atactccaga aaaacaaaga cgttttgctg gtgaaatcaa aaaaatgcaa gataaatggc
    22561 cagcatatat tgcgcatgat ccattttaca atgataattt aacaaaagaa ggaatcggcg
    22621 acttttcgtt gagaccagat taaactataa
//
LOCUS       DACDYJ010000043        22410 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDYJ010000043 DACDYJ010000000
VERSION     DACDYJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.043"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            144..542
                     /locus_tag="IWM51_001730"
     CDS             144..542
                     /locus_tag="IWM51_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9971153.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1221
                     /locus_tag="IWM51_001731"
     CDS             526..1221
                     /locus_tag="IWM51_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP9971154.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1815..2702)
                     /locus_tag="IWM51_001732"
     CDS             complement(1815..2702)
                     /locus_tag="IWM51_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP9971155.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2887..3726
                     /locus_tag="IWM51_001733"
     CDS             2887..3726
                     /locus_tag="IWM51_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9971156.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3922..4842
                     /gene="sgrA"
                     /locus_tag="IWM51_001734"
     CDS             3922..4842
                     /gene="sgrA"
                     /locus_tag="IWM51_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP9971157.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSMSSESSTGSTSSESSTGKVN
                     NELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQEKAKETDNRKSDLPETGSR
                     TLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5140..5496
                     /locus_tag="IWM51_001735"
     CDS             5140..5496
                     /locus_tag="IWM51_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971158.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5499..6656
                     /locus_tag="IWM51_001736"
     CDS             5499..6656
                     /locus_tag="IWM51_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971159.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6682..7122)
                     /locus_tag="IWM51_001737"
     CDS             complement(6682..7122)
                     /locus_tag="IWM51_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9971160.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7282..7788
                     /locus_tag="IWM51_001738"
     CDS             7282..7788
                     /locus_tag="IWM51_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9971161.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7956..9868
                     /locus_tag="IWM51_001739"
                     /pseudo
     CDS             7956..9868
                     /locus_tag="IWM51_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
     gene            9873..10874
                     /locus_tag="IWM51_001740"
     CDS             9873..10874
                     /locus_tag="IWM51_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9971162.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(11076..11282)
                     /locus_tag="IWM51_001741"
     CDS             complement(11076..11282)
                     /locus_tag="IWM51_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP9971163.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11928..12776)
                     /locus_tag="IWM51_001742"
     CDS             complement(11928..12776)
                     /locus_tag="IWM51_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9971164.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12940..13842
                     /gene="gnd"
                     /locus_tag="IWM51_001743"
     CDS             12940..13842
                     /gene="gnd"
                     /locus_tag="IWM51_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP9971165.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13844..14845
                     /locus_tag="IWM51_001744"
     CDS             13844..14845
                     /locus_tag="IWM51_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9971166.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14842..15126
                     /locus_tag="IWM51_001745"
     CDS             14842..15126
                     /locus_tag="IWM51_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9971167.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15159..15956
                     /locus_tag="IWM51_001746"
     CDS             15159..15956
                     /locus_tag="IWM51_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9971168.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15970..16803
                     /locus_tag="IWM51_001747"
     CDS             15970..16803
                     /locus_tag="IWM51_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9971169.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16821..17678
                     /locus_tag="IWM51_001748"
     CDS             16821..17678
                     /locus_tag="IWM51_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9971170.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17700..18209
                     /locus_tag="IWM51_001749"
     CDS             17700..18209
                     /locus_tag="IWM51_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9971171.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18246..18647
                     /locus_tag="IWM51_001750"
     CDS             18246..18647
                     /locus_tag="IWM51_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971172.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18759..19112
                     /locus_tag="IWM51_001751"
     CDS             18759..19112
                     /locus_tag="IWM51_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971173.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19146..19589
                     /locus_tag="IWM51_001752"
     CDS             19146..19589
                     /locus_tag="IWM51_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9971174.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19606..20691
                     /gene="uxuA"
                     /locus_tag="IWM51_001753"
     CDS             19606..20691
                     /gene="uxuA"
                     /locus_tag="IWM51_001753"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9971175.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20684..21397
                     /locus_tag="IWM51_001754"
     CDS             20684..21397
                     /locus_tag="IWM51_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9971176.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            22149..22349
                     /locus_tag="IWM51_001755"
     CDS             22149..22349
                     /locus_tag="IWM51_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9971177.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7724 a         3442 c         4624 g         6620 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata
     1081 acgttaggta aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg
     1141 aggggcttcc aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa
     1201 aaggtcgctg attacttctg aatcaatttc tccataaaat aaacgatagc cttctcgaaa
     1261 actcgaggag gctatctgac gattcaatat tcattaattt aaagtttggg tgactattac
     1321 taactattac ttatttctca tttaaacgat gtattcccta acctctaata catccttgta
     1381 actctgaagt tacgccagtt ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata
     1441 aaaatatctt tcgtgtttct ctagtataac agaataagaa gaacaagtcc agaagggata
     1501 tttttagtct tttttaaaat aacttttcag tcgagtttga gtttatgata gttgtttttg
     1561 aagcgatacg gcaagtgaaa aaatagggaa gcatgaaaga atctacttaa tggcgcattg
     1621 attcatgtga tagaaataaa aaactttttt aaattatatc ctcatgattg taacaaatcc
     1681 agtgatttgt tttggtacaa ataaaaaaag gccccgatga agttgtggca aaagtggttt
     1741 gcctcaaaaa taagaaggaa tttccaaaaa tcacttctca aattttgcca catccccaat
     1801 tgggtcaaat ttgtttattt gaatatattt tttacataag gatctttaat agttacattg
     1861 tcttttttca tcactttacg gattgtttta tccatgaatt ccgtatcaag agtttttgct
     1921 tcggtagcaa ttttttctag ctcagatttg tatttgtctt tattagaacc tttatcctgt
     1981 ttttttacca tttttactag ataatagctt tgctgataag tcgttggatc tgttacagga
     2041 atgatatcag aaacttcccc gttttttagt ttaaatgctg ctttttttac ttcagtagga
     2101 atctctggat tggttgaatc aaaattcatt tttccatcag tttcctttaa tgttttattt
     2161 tttccataag cttgaacaag cttggagaaa ttttctcctt catttgcttc tttttttgct
     2221 ttatctgctt cttctttagt cgaaaataga atgatctgtg tactaacttc tggatagaat
     2281 tctttccaag cagtgtctag atctttatct gttaatttta cattggcttt caaaccgtat
     2341 tggaaagcca gcatctgttt aaagagtttc ttatatgtct cttctgtttg gccaactgct
     2401 tttaattgtt ccttgtagtt tggaccgagt tttttgattt gttcattata ttcttttgtt
     2461 acttcctttg aagaaatctc ttttccgtat acatcatcaa atgctttgaa gacaatcatt
     2521 ttttttaata gtgtttgatt atttgattta acttttgggt tatcataaaa atcctgaact
     2581 gtaagtgttc cacctttaaa cgttgcaatc gtttcattgg acttgctaca acctgagaat
     2641 agtaaaattg cacatgctga aaggcacata attagtagtt gttttttcaa aaaatttccc
     2701 atcctttcct tattaaaaac agtctaacag aagaattagg gagacagtct ttcattttcg
     2761 taaggttgct gtagatattc ttagttcgta aaaatctatt ctctctatga tggagaacat
     2821 ttacatagtt ttcacgagac aagaaagaaa agggttgtta aacttaatat aaaggaggga
     2881 tgaaagatga tcaggattat tcggttgatt ggatctgttt taatagtctt agttggtttt
     2941 gttttaggaa ttcttgtcaa taatgcactt gcggatatgt ttattggaaa tagtgaatgg
     3001 attggagcaa ttgccggtac ggtcggacat ttagctgttt ttttactagc tttatttctt
     3061 gctagtaagg tcgaaggaaa aagaatagaa gattatggtg tttctgttcg aggaaaagac
     3121 tgggggtatt tagcgggagg tctcgttgtt ggaattggag tgtttctgct tattactagt
     3181 ccattatatt tgacgggggc gtatcggtta gattcaggaa acgcaaacat tgtgccgctg
     3241 ataacgagtt ttattttatt tattgcagtg ggggcatcag aagagctttt attccgtggc
     3301 ttttttcagc atcagatgct tagatttggt ccgttaatag caatgattgg atctgctgca
     3361 ttatttgctc ttttgcacgg attgaatcct aacatgacat ttctggcagt tttcaatatc
     3421 tttttagcag gttgcttttt cagcgcactt atctacagta caggaagttt atttacggct
     3481 attggtgctc atattacttg gaattggaca caaggagcta ttttgggtat accagtcagc
     3541 ggaacgcaag aacaaggttt tttctccact ctgatccaat cgcaaaatac aataattact
     3601 gggggaaact ttggggctga agcatctata tcaactacat tattcttgtt agtcttgatt
     3661 gttggtttgt tactctatgc atataaaaca aagcggatga agaaatacac tgctgtttat
     3721 caataaaaga tgaagaaagc aaggctgata tcggtcttgc ttttttaagt ctgtcattga
     3781 ttaaacgaaa aagtaaacaa attcaagcga atacacgaca tcttttgtta gattttcaca
     3841 actgactgtc gtgttctcat cattttcatt ttatataatt cctttatcaa atagaaggta
     3901 attatatgga gagtgataga aatgaagaaa acagcgaccg tattatttgt tattcttggg
     3961 ctgagtataa ttgcagcaat gattggaatc agtacttctg caaatgaacg ggcaaatgag
     4021 aatctttcat ttactgtgaa aactgatcgg attgtttatg acatgacaca acaagtcatt
     4081 actattccag tcaaaccaaa caaaagcgtg aatgctagtg atgtacatgc tgttctaaca
     4141 tatggttggg atggtaatgg aagttcagaa aaagttattg gggaagttta tttaaaagac
     4201 gtacaatgga ccgctggtat tgagtataca ataatgattt ctgcagaact atcgattgat
     4261 gagatcaaga gtaaagataa agttgatttg attgtttttt atgatggaca gatgacgatc
     4321 acggagaatt tgaaaccaag cagttggaca gttgttggac cgccaagcag tacggatagc
     4381 acaagtagtg aaagcagtac agaaaacacg agcggtgaaa gcagtacaga gagtacgagc
     4441 agtgaaagta gcacagaaaa cacgagcagc gaaagcagta cggagagtac gagtagtgaa
     4501 agtagcacag gaagcatgag tagtgagagt agcacaggaa gcacgagcag cgaaagcagc
     4561 acaggaaaag taaataatga acttgatttg gtacctatca taccaactaa ggaacaaaag
     4621 agaaatgcag ggagcaacct aaaaagtgtg ctaaaacagc ctgattttgt ttctcgtgga
     4681 attgatcatc aagaaaaagc gaaagaaaca gataatagaa aaagcgattt accagaaact
     4741 ggtagtagaa ccttgaacaa gctaattaca tggatgggtg tgttactcat tttgattgta
     4801 ggagcaagct attttcgttc tctcttccat cgagtgaaat aaagaataaa agcaacagat
     4861 ctaaaattga ataaaacgat agatttttga gtgaatattt cattcgagag tgctgtattg
     4921 cctggcgaag ttgtagcttt gccagatcta tttttgttta ctaacatatt tgagaaacag
     4981 gaaataacag gaacacaaat ggaaaataag agcaagaata attgacaatg cacactgcgc
     5041 atggtagtat ctctgaaaaa tcagtaacga atctatttgt acgttattca tgtagctgtc
     5101 ggaataagtc taaataatag ataaaaagga gtatgtactt tgtttgaaga atttattgat
     5161 atcaatgaaa ggcaagtata tcaatttttg aactattgtt atgaaagaga cgagaaatta
     5221 tatgtggtga aggatatcgc tttagatcta aactacaccc tggctaagat gaattcggtg
     5281 attcaacaag cagaatcgtt ttgcgaaagg tatccagaat ataaattatc ttttctttca
     5341 gaaaacaaga tgataaaagt cgagttttct tctcagtttt tactgagtaa agtttattct
     5401 atacttttag agggaacgat tggttatatt ttactggatt cactttacaa aggtacttat
     5461 caatccttag aaaatcttag tcaaaaaatt atataagttt gcgtacgatt caaagaaaat
     5521 taagagaact taatactatt ttgaaaaact atcatttatc ttgcagcttg aaaaaaggta
     5581 atccgataga aggagaagaa taccgtatcc gatacttttt tcacttgatg tattggcagt
     5641 cattcgacga aaatcattct gatatattat caagtataaa aaataaacgt caactattag
     5701 aaaaatttgc gaaacatgcg ccatatacgc gtagtataga tagaaagaaa tggatcagcc
     5761 tactaagaat ttcgttgcaa cgaatgaaac aaggccactc gattacgtat gtcccagaag
     5821 agatgaacaa atttgtgcat ccattgataa caaaagaaga gtttacgcat tacatactag
     5881 agcctttttt tgaagcaaac ttctggtcga tgaatcacat cactccaaca gagcaagctt
     5941 acctttattt tatgtttggt gtaatgaaca cctatttgga agaagatttg ttggatactc
     6001 cagataaagt acaagaaagt tggggctgga ttgaatcgtc tattgtagaa aattttgaaa
     6061 aaaacttcaa gatttgtttt acaacttcag aaaaaaatta tctatatgtc aatttggcaa
     6121 tgatccattt gtattcaagg gtttttggga caaaaaagaa ggtggatgct tttggaaaat
     6181 cagcagaaga cagagatttt cagcatatat ttccagtgat gtatccaatc atgcaacagt
     6241 tttttagtgg ggtagctcgc agaattccag agcttaatac ttattatgaa aagaacagac
     6301 ggttgatttt tcaatactgt atgttagtca gggatatttt tataaaacac gaacaacctg
     6361 tcatattcta tattcaatca aagtatggta aaacacagga attatgggca aagaaaagga
     6421 tattatctat gactgaacgc ccaataaaat atagtgcatc cgctgatcag ttacctgata
     6481 ttgtcattag cgattatcct atcgacaaaa aggcatatga tcaaacgaat acacagatat
     6541 tttactggaa tggacaacct acaaaaaatg attgggcgcg tctgaaagct gaaatcataa
     6601 aaatcaggaa tgagaaaaat agatccatat tggaaaaatt ggatttcgtt aattaagaaa
     6661 agcagattgt ttatgccaaa attaagacat tgcaaaaaga ttattttctt taaaatcgac
     6721 aagatgacca gaaagcaaga gttcgttgag ctcttgcttt cttttagttt tagaaaggaa
     6781 catcctgatt ttatcttcgc taaatccagt caaaagtttt gcagattggt cgtagataat
     6841 cggttttttc aataaagaag gggatgtacg aatctcaata tatagttcac tcaaactgag
     6901 ttcatctatt ctgtgaacaa gatttttata actaatagaa tgagtagaga gcatttcggt
     6961 cacgccgttg tttgtcaatt ttagtaagtg gataaattca tttttggtta gtggttgacg
     7021 aattaagttc tgttcgataa acggtatatc atggacgata agccaacgtt ttgcgcgtct
     7081 tgaagacgag cagccagaac ctgtatacaa tttaatcatc atacattcct ccaatagcta
     7141 agtatttatg aaataataac atgaagaaat agtcgaaaag tgccatttag aggtgtaaaa
     7201 taagaaaatg ttgtcacgta taatggaact agctagataa gaaatactat taaatcaaga
     7261 aaaaagaaag aaggcacaag tatgtcaaac acagccgacg caatagtagt gatcgattta
     7321 caaaaagggg tatgttttga ttcttcgccg atacacgaat tagaaacagt gacagcatta
     7381 gtgaataaaa gaatcaaaac atacgaaacg attgggaaac cagttatttt cgttcaacac
     7441 gaagatgctc aattagtgcc acaaacaaaa ccatgggagc tattgtcgga attatctatt
     7501 cctgagcaca gttattttgt gagaaaaacc catgcgaatt cattttataa gactaatttg
     7561 agtgttttat taactgaatt ggactgtcat tcgatcgaat tatgtggggc acaagcagag
     7621 tattgtatag atacgactgt gaagtttgct catggattag gataccagct tatgatgtgt
     7681 agaggagcaa cgacaactta tgataatcct tttatgaaag caaaggaaac aatcgctttt
     7741 tacgagaaaa tttgggataa acgatttctg acatttttgg agcaataagt tgtttcttcg
     7801 tttaaaaaaa gcagaatgga tactggttta tccattctgc tttattacgt aaaatgagaa
     7861 aataatcctg ttctattttt atgggataaa ccatttattt tttgtataaa aaatgtttgc
     7921 gtttacatat aattctccta gaaacataaa aaggagtgaa tagcttgaga acaaaacaag
     7981 tattaaaggg agcgttggtc atcttatttg ctgtaagtac aagcgtcccg caatggacag
     8041 tatatggaga gagcatttct tccgaatcgg aatctcggga ggtagaaacc acagaaacta
     8101 ctgatgctgg aaatgaaacg aaatcgagta cttcacaaac agaaacaaat actgaaacaa
     8161 cggttgagac atctgttact agcacagatc aagaaacgac cgctaccaca gataaccaac
     8221 aaacagaaga agcaacagct acaaaaatgt caaacgacga atcctcttct gctaaagtaa
     8281 gtgacaaaga gaatgggaca gaaaatgatg atatcggtaa aggtgaaaat ttcaatggta
     8341 aagaatattt gaattacttc ggtaaacaga tgacagaaat acttaatgga ccaacagaag
     8401 acatcaagtc aaaattaata gagttattag tcactgagct tgctgttaat gattatggtg
     8461 tagataaaga gattatcaaa catctgattg ctacttatct attgtacgat atcgcttttg
     8521 caaaaaacag tgtgttgatg tcgatgcttg gaggtacaga tgatgggaca acgcctacag
     8581 aaaacacagg cttctatcca aatacaaaac aacttcatca aactgtacta aacaaacaag
     8641 atggcaaaat gatggatgta tatgcatatt atgtcgatca aggttcagat aaaacagtca
     8701 tgatccatgg cggatttcga gggaattgga ataacgggat cgttacagaa gaatacaatg
     8761 atttttataa ggccgggtat aatttattat ttgttgattc cagagcgact ggtaatagcg
     8821 gaggcgatta tgtgacctat ggacaatatg agtcagatga cgtcctttac tggataaatc
     8881 aagaagttcg agagcggcca tctcaaaaga ttttacttta tggtggttcg atgggagcag
     8941 ctacgatgat gtctgtatta gcgaaagata ttcctgtcaa tgtcaaagga attattgaaa
     9001 attgcggatt tgcatcaatt gatgaacaac tacgttttac gtacagccag acagtagccc
     9061 ccgcactacc ggatgccata aaaaatcagt tagacatcat tggggatcaa gagcatgaag
     9121 atttatttat gggccttttg aaacaatact attttgatca ggagatgcat ttggatacaa
     9181 aagctgctct acctacgatt ggaatgtcag attcattgcc taaattgatt atccatggaa
     9241 cagcagatga tgtagtacct gtgtctaacg cgcaaaaatt atatgagtta tcaggagggt
     9301 ataaagatct tttattagta gaaggagcag ggcatggaaa ggcacaggaa gttgaccatg
     9361 ctgcctacac gaaacatgta acggactttt tgaaagtagt gttccatgat cagataaacg
     9421 taaagtatgt ggatgaaaac aataaaagtc tactcaaaga ccaggacgaa atccagcttt
     9481 acggagctta tggagaaaat tatatgacag agcaaaagac attcgaagga tacgaattag
     9541 caaatgttga aggaccgact gaagggattt ttaatgagac gataccaact attattttct
     9601 attacaaaaa aatcccagta gctccaccaa aaaagcaaga tccgacagag aatgcagaca
     9661 ataagggaaa gaatgaacct cgagaggatc aatcaagtga tgatacgcaa ccgattcaga
     9721 tgacagtgaa agtacaaaaa aagacagatg caaaaaaaga atgttgccga aaactggaga
     9781 aaagaaacat tcgcttctca tttattcagg tggattttta tctgttagca cgattatcac
     9841 atggatttgg cgaagaaaga gagggtaaat gaatgaaatg gttcaagcga atagtgattg
     9901 cagtaccagt tatcatcgta gttattttag tcactgtatt tttgattaat caaatcacac
     9961 caaagccgat tagcttgctt gttagaaaac aatttgacac aagtgaaaaa gaacaagtat
    10021 atgcccgacc agatgatttc cagcaaaaaa tcgaacaaat cgatataaaa aaagaaatat
    10081 cctatccttc aaagtacaaa aataataata tggatatcta cacaccgaaa gtggaaaaga
    10141 aaaaattacc gattcttttc tggatgcatg gaggcgcata tgtaggaggg gataaaaatg
    10201 attgtcgtga ttatttggaa tatctatgtt cagacacgca gcaaatcatc gtaaatatag
    10261 attatgaacg atcaccggaa gcaaaacatc ctacaccagt tatccaattg aacgaagcca
    10321 ttcaagcagc taaaaaagag ataaaagatc aagcagattg gtcgaatatt tcgattggtg
    10381 gaaattcggc aggtgcacaa attagtggag aatatttgtt agcacttcaa aatgaagaaa
    10441 ttaaaaaagc tgatcatctt gatccgactt tggagaacaa gcagattacg aagtttattt
    10501 ctttgtctgg tctgttggat ccttctgcac ttactcaagt aagtgataaa atctctagct
    10561 ttctttatga aaaatgtgga tgggcttact ttgataataa ggactttgaa caatcagaga
    10621 aggtcaaaag tttagccttg gtcagacatg cggatcgttg gacgcaaaac acattcttaa
    10681 cggatggaaa tacaaatacg tttacgaaac aaatggaaga agtaacttct gtatttgaga
    10741 aagcgggggt gaaagtcaca aaagtagatt atgcggagaa aaatgctgtt ttgaaccatg
    10801 aatatcagtt tgatttcagc aataaacaag cgcaagaaac atatcaaaag ttagtaatgt
    10861 ttttcaagga gtaaagtctt atgatgcaaa agaggttgta atctgtcact tagctccaag
    10921 tcacaaggag caatttcaat aaacaagctt ttcatggttt atgaatattg taacttgtga
    10981 ctgaggagtt agagatggca aggggcacct tttattcgaa atttgggaat atcacacgat
    11041 tattgtcacg atctctttta cgtaaatttt ataagtcaga tttgttgttt tgatcgttta
    11101 aaaaaagcaa caaatccgaa gcctgcggag gcaagaaggg acaaaaagcc gagtggtaag
    11161 caaaagaatg ctggttccac taatattccg ttgttattga tataaggctg ggaacaaaga
    11221 tgaatagcaa ataaaatcaa tgcactagcg aaaaaaagca aagaaatatg gatagctttc
    11281 ataagcagca cctccttttt tataattgta tgataaatag attaagaatc tataaatgtg
    11341 acaagtttgt taggatgtga ataaaatgat catctagcga acttgaaaca aagatggcta
    11401 atgataagca attaatgatt cagcaatcag ataggtacaa agtttaccat catacgtcta
    11461 gaccaataat agaaaatatg ggaatataat agaaaagatt taggataccc ccaaaagaat
    11521 ttccccaaaa attcgatgta tcctaaactt ttttatttta cataacaatc atatcaactg
    11581 tgaaataaaa ttacaaatga taacctaaaa tgaggccaaa agccagtgtt atcaaggaaa
    11641 tacttaattc accaaaattt tttttaattt tttagaaaat aagaaacaaa aataataaaa
    11701 aaaagaaaag ataaattttt tactactgta tactaacttt gttatcgcat ctaacaagta
    11761 actcgaataa taccaataca accttctcca aagatgcgat gacatacgtt ctaacccacc
    11821 gactattgat gttctgtacc acccttgcag aaccaaaaaa gccactacca aatatttggt
    11881 agtggctttt tttgttttat ggatttttcg ctttttctgt tatggtgcta ttctttgatt
    11941 tcagaaatag tatgtcgaac tttagcgata gaacgacgtg attctttttc gttgtaaaac
    12001 atcaaaatca catatagaga gtccaaaatg cttagttgag aaatacggga agctaaagct
    12061 tcagatcgga attctgtttc ttcagaaata gaaataaaaa cgacatcgcc aagtttagca
    12121 agggaagaat tagcttggct agtaataacg attgtttttg ctccagtttt tcttacggtt
    12181 tcagcaatat ggattgtttc ttttgattgc cctgtgtgag aaatcaaaac agcacagtct
    12241 ttttccgtca ataaagaagc ttccatcaat tgaatatggt aatccgtgct gtgagaagtt
    12301 gcgatagggg aacgcaaaaa tttatgataa gcgtcagttg ctacaatctc tgaaccgcca
    12361 acaccgaaaa aatagacacg tttagattca ttgataatag tagccgcttt ttttagatcc
    12421 ttttgttgta acaacttttt tgtgtcattt aaagttgaaa tactagactc gaacaccttt
    12481 tgcgccatag tgagctcagt atctgttttc tgaatatttt cgtggataga aatagcagat
    12541 agatcatttt cttggatcaa catggccatt ttaaaatctt taaatccatt ataaccaagt
    12601 ttttttgtaa attgaaaaaa agtagagtca gcaatgccca gttcacttgc taaattgcta
    12661 ataggactat gacttacttt gcttggattt tctaaaatat aatcggcgat agcctgttct
    12721 tttgggctga agtcgttgta cattgttttg atttgtagtc ttatggagac gctcatgaga
    12781 attcctcatt ttcataataa gattatcatt ttttccgttc tcattaatgt taaagataca
    12841 aggagagtag gaaaaaatca aacaaaaaat atttttgtaa aatctattta caaaaaaatt
    12901 tttttgtata atgagggtat aagaaatgga ggcgctatca tggaaattgg tgtaattggt
    12961 ttgggaaaaa tgggattgaa tttagcttta aacttaaaag agcaccatca caaagtacaa
    13021 ggactcgaca tttcaagtac agctgttagt caggcaagag aaaatgacat tcctgcttat
    13081 caagagatct ctgaatttct agctactttt gagcagaaaa aaatcatctg gttaatgctt
    13141 cctgctggag agataaccga aaacatgtta agaaaacttt ttccaaaact cggaccagga
    13201 gacattatta ttgaaggcgg aaactcttat tataaagatt caatccgtcg agcagaagag
    13261 tttcagaaaa atgaaatcgg ttacttggat tgtgggactt ctggaggaat cgaaggagca
    13321 cgacatcaag cctgcttaat gattggagga gataaagaaa cttttctatc cgtggaacaa
    13381 ttattcaaag atgtagcaat tgaaagagga tatctgtatg ctggtgcatc cggaagcgga
    13441 cacttcttga aaatgatcca taatggaatc gaatatggaa tgatgcaagc aatcggtgaa
    13501 ggattccaac ttgtacaaaa aagtgagtat gcttttgact tggaacaagt cgcacgtgtt
    13561 tggaatcatg gttcggtgat tcgcggctgg ctgatggaaa tcatggaaga tcagttaaac
    13621 gaacatccca atttaagtaa ttatcgggga atcgtttctg catcaggtga agcaaaatgg
    13681 acggttgaaa cagcgctaga aatggctgtt ccagttccag taattgcaac tagtttgttc
    13741 atgaggaatc tttcacagga agaagatagt ttttcagcaa aagtagtttc agcactaaga
    13801 aatgggtttg gcggacatga aatagttgaa aagggtgagt aaaatgccag attatcatat
    13861 agcaatcgtt aattccagca gtttcggaag agtctttcca caccacttgg aaagattaaa
    13921 gtcagttgga gacgttcatt tctttactgt tgatcaagag attccaggaa aagaactagc
    13981 ggagttactt aaaggattca atatcattat tgcaagtgtt acgccatttt ttcgacgaga
    14041 tttttttgaa aacaaagatg aactgctatt gatttcccga catggtattg gttacaacaa
    14101 tattgattta aaagcagcaa aagaacacca gacaatcgta tctatcatac ctgctttagt
    14161 tgaacgaaat gctgtagcag aaaataatat aactaatttg ttgtccatgc ttcgacgcac
    14221 gacacaagca caagcaagag tcaaggacga tcgatgggaa gatcgggcgg agttcgtcgg
    14281 taggacgctg tttaacaaaa cagttggtgt gattggtgca ggaaataccg gaagcggagt
    14341 aatcgaaatt ttgcgtaacg gttttcgctg tgatgttcta gcttatgatc cttacaaatc
    14401 agatttatac atcgaaagct ttggtgcaaa aaaagtagaa cttcaagaat tactggaaca
    14461 atcagatatc atttgtttat gtgcgaattt gacagaagag aactatcaca tgatttctga
    14521 taaagaagtc gatcagatga aagaaaatgt ttatctatct aatagtgcaa gaggcgctct
    14581 agttgatgaa gaagcgatga ttaatggtct gaaaagcgga aagatcgcag gatatgccac
    14641 agatgtttta gaagaagaac cgggacgtca agatcatccg tatttagcgt ttgaacaagt
    14701 agtgattacg ccacatatat cggcctacac aagggaatgt ttagaagcaa tgggtgaaaa
    14761 atgtgtcaga gatgtcgaac agatcagtca gcaccttctt cctgaacgtt ctgtccagac
    14821 aaagagcaac tatataaaag gatgatgaag aaaatggtga acggtttaaa agtgaaaaca
    14881 ggccctcaat tttatttata tgaagaaggt ggtatttcaa aagtttctga tctcttgaaa
    14941 tcatacggcg cgaaaagagt actggtcacc cacggaacgg tatcatggga aaaggcgcta
    15001 cctaaattag tatttttgaa tgacgaaacg attcaatttt tttatcatcg ttattctgga
    15061 gaatgtagtt atgctgaagc cagacgaata gctacaatca tcaaaaaaat gaaatcgatt
    15121 tcgtagttgg cgttggcgga ggaaaactga ccgatctagt gggttatagt gccaatcttg
    15181 caagaatcga tttcggagtc atccctactt tagctagtaa ttgtgctcca tggacacctc
    15241 ttgctgtcat gtataaggag aatggacaag cagaagggaa aacagaacac ttttacagac
    15301 aagctgcttt ttttataaca gatccccaat tagtgattga ttcccctgtt ccatatttca
    15361 ttgccgggtt agcagataca ctagcaaaat ggtatgaatc tgagctcatc ttagaacaag
    15421 attttttgca agatgaatca tttttgaagc tagcacaaga tactgcgaag atctgcaaag
    15481 aagagatttt gaatcattct gctaaagcaa tccaagatat ggcacaaaga aaactgacat
    15541 cggagttcaa acagctttca gagattatct ttgctgttgc tggtttagtt ggaggattag
    15601 gagataaata cgcaagaaat gcggcagcac atgctatgca tgacgctatc agtaaatata
    15661 ttccggaaag ccatcgtttt ctacatggag aaaaagtagc gtatgggatt ttctatcaac
    15721 tggcactaga aggtcgctgg gaaaaaatag acgagttata tcctttgtat cgggaattag
    15781 aactgccggt ttctctagaa caaatgggat tatttccgaa agataaacag ataatagata
    15841 aaatggctga atttattgat tcgaaagaga aagtgcattt gattcctata gaggtaacac
    15901 agaaatcact gatacagtca atgatggaac tggaaggtta tatcagaaag atctgaagga
    15961 ggaggacaca tggaaacatt aagtgtagta caaagtttgt tgattgcttt atgggtagcc
    16021 gcggttatgt cccgctggtt aggtggagga gccacgttga ccttacgttt ttctcctttg
    16081 atgactggat tagtcgtcgg gttgatcatg ggggaagttg cacaagcgat gattgttaca
    16141 gccgctttgc aaatgattta tatgggggtt ttttctccag gaggatcaat gccagctgaa
    16201 ccatctattg cagcagctat cgcagtacca gtcgcattat taggggatct gtcgccagaa
    16261 gcagcgattg ccgttgcggt gccagtcgga ttgctcggaa gttacttgta tcagtttcgt
    16321 tttttcatca atactttttt aggtaaatat acagacaaag ctgtagaaga tttgaataca
    16381 aaagctatgg cccgatcaat tattctttat ccaacggttg cctcattttt attattcgtt
    16441 ccgcttgttt tcattgcttt atatttaggg gcgccattga tcgctgatgt aatcaaagct
    16501 ttagagggaa cagtcgttat ccacattctt gaagtagtag gtggagggtt agcggcaatc
    16561 ggtatcgcga caactgtata cgttatcggt cgaaaagatt atctggtctt tttcttcctt
    16621 gcttatttta tgagtgttgt tttgaaatct ttagatatta cgatggttac ttatgccatt
    16681 tttgggatta ttatcgcttt gatctttgtc cagtcgcaaa aagggaaaca agtacagcct
    16741 gccacaagca ataacaattc agcatctttt gatgacgaag atgactatga tgacggattt
    16801 taatttagga ggaatgggaa atggctgaaa taatggataa tcaaacaggt gctcctgaag
    16861 aaatcacgaa aaaagatgta acaaaagcct atctgagatg gcattttgca aacgaaattc
    16921 ctcactcttt tgaacgttac ttggcgccat cactactata cggaatgatg ccaatcttga
    16981 aaaaacttta taaagatgaa gactctttaa aagcagcata taaacggcag ttactgttct
    17041 tcaacactca attaagctgg ggtggcggtg tgattactgg cctaatgtct tctatggaac
    17101 agcaaagagc agaagaagag tatgaagaaa aagagatctt gatgcaggat gacttgatgt
    17161 ataacacgaa agcgggattg atgggggcac tagcaggaat cggagatgca atcgattcag
    17221 gaaccgtcca atatattttt atcgcaatcg cggtaccttg ggcacaacag ggaagtcctt
    17281 taggcgcact ttttcctttt atctgctttg ggttgtatca ggttttacta ggggtatttt
    17341 tcgcaagaca agctttcact atgggccgta atgctacagg cctaatgcaa aacactggtg
    17401 ttcagacagc gattgaaatg ctatcagttt tagggttgtt tatgatgggg gtgcttgcgg
    17461 gaaactatgt aaaagtagaa tcttcattga aatttgcgat atccggacgt gagtttgtta
    17521 tccaggaaat tcttgatcaa atcgtgccag ggatattacc tttagctgtc gtaatgggtg
    17581 tgtacttgtt ctatacgaaa aaaggattga aagtcacaca agcattatta tggctgactg
    17641 gaatattaat tgtgctagca ggaatcggta ttttataaaa agaagggaac gtgaataaga
    17701 tgggtgtagt caatttagcg cgtgtggatg agcgtttgat ccatgggcaa gtaatggtaa
    17761 cgttatctca aaaaagtgga gtgaattcca tttttgttgt agacgaagtt gtagcaaaag
    17821 ataaatttat gagagatctt tacaaaaatg caggaagccg tacaggacaa aaaacaattg
    17881 tcattacacc agagaaagct aaattctatt gggatgaata tcaatttaaa gaatacaatt
    17941 gtattttgat cgcaaaaaca gtccaaacga tttacgactt agtaaaacac ggaatcccca
    18001 tggaagaatt gaatattgga gggattgctc aaaaaaatcc tgatacagat cttttagtga
    18061 cgaaatctgt ttatttaaat aaatcagatg ctgaaaaatt gaaagaaatg aatgaacaat
    18121 atggcgtaaa gaatatttat tttcaagcca ctccatctgc acctaaaaca ggattaaaag
    18181 atgtattgaa caagtttgat ttgtcataaa caaatgctta gaaaaaagat aaagagggcg
    18241 aaaaaatgaa gaagaacgag ctgtttcgtg attgggaatt tcgttatcgc tatatttacc
    18301 gtaagcgccg tacaaaaaag agtaaacagc gttttctttc agcattagta tcagatattt
    18361 acagtatgag aacagatgtg acagtcattg cttacgatac gctagcctac cgatcaaaaa
    18421 atatttatgt tggagatatc gaaaaagcag agaaagttat ctgtacttat tacgatacac
    18481 ccgttcatgc attaggaagt tatttcatgt ttgattggaa agaccaaagg aaaaaaacaa
    18541 tctactctat attattgtcc ttcattttgt tgttcagttt aggttggtgg ggaatgatga
    18601 tatataacaa aaatcctcat cacgtattcg atctgctatc cgtataaaca ggtatcactg
    18661 tactagcatt tgggagctac tttttcttat tgggaaaagc agctcgcgga tggcctagca
    18721 gacaaacttt tattcgaaat acttcatcca tattaacgat gctagaaatg atccgtacga
    18781 ttgatgatcc gagtgtagcg tatgcgtttg ttgatgaagg ctgttatgga gaaaagggct
    18841 tagacagcgt gagatcgggt atgaaaaaag aggctattct tttttatcta gactctgttg
    18901 gggcagatac accgctacag ttttcgggaa attatttttc aaataaagaa cagtggctga
    18961 aacaagttga taagttgaaa gaaaaaaacg tcaattatat ttttagtgca agaaaaaagc
    19021 aagcgcagtt cttctatttg accaaaacag atttacgagg gaaaacattt aattggcaaa
    19081 atgccaatca gattattgct ctttttcgat aagtaaaaag tgcaaaacaa ggagtgagaa
    19141 gactaatgtt ggaaatcatg atcgttacac atggtaagtt gagcgaagga ctgaaagatg
    19201 cggcggaagt aattgtcggg acatcgagcc atattgaaac aatgtgctta gagtctgggg
    19261 aggacatcca aacattcgga gagaaggtca aagaaaaatt aactatgatc aacacagcag
    19321 aacccatgct agtatttgtt gatctaatta gcgcaagtcc gtacaatcaa gcgctcttgg
    19381 cagtcaatga actgactgaa gaaaagaaag cagccactta tgtcataggg ggcgtgaatc
    19441 tgccaatgtt gttagaagca atcaatcatc agatgcttca cacaccgata gaagaagcgg
    19501 tggaacaaat tgtcaaacaa ggaaaagata atttaactgt ttggcatgct tcagcggaag
    19561 aaaaaaaatt agatgaggac gatttttaga aggaaggctg aaaagatgaa gtggggattt
    19621 agatggtatg gtgaaaaagg ggattcgatc ccattaaaat atgttcgcca aataccggga
    19681 ataaatggga taattggaac tttgctgact aaaatgcctg gagatgtttg ggagatttca
    19741 gaaattgagg agttgaaagc atctgttgag agccaaggac tggaactgct tggaattgaa
    19801 agtgtagcga tccacgatgc aatcaaggca gggacagatg aacgagatta ctatattgat
    19861 aactatatac gaacaatcaa aaacttgtct cgatgtgggg ttcgattgat ttgttatagc
    19921 ttcaaaccaa tttttggatg ggcgaaaact gatttgttta atgaaaacaa cgatggaagc
    19981 tattctcttt tatacgatca gaaaatcgtc gatcagatgg agccaggaga aatgtatcaa
    20041 ttgatacaca gccagtcaaa gggattcaaa ttgccgggtt gggaagaaga acggttgaaa
    20101 aaattcgctc agctggaaaa actgtatgaa ggaatgaccg aagaagaatt atttgaaaat
    20161 ctgacttatt ttttagaaaa agtgattcca gtgtgcgaaa aagcagatgt aaaaatggct
    20221 atccatccag atgatccgcc atgggaactt tttggttttc ctagagtgac gaaaaatctt
    20281 ggcgacttga agaaaattct aaatgcagta gattctccat ataatggact aacgctgtgc
    20341 acaggatctt taggggcaaa tccggaaaac gatttagttg aaatcatcca tgaggtaggt
    20401 tcgcgcatcc attttgtcca cttcagaaac gtggagtttt taggagaaag aaagttcaaa
    20461 gaaacggcac atccctctac agaaggctcc cttgatatgt atcatattat gaaagcgttg
    20521 gtgcaagaag ggttcgacgg tcctatacgc cctgatcatg gtagaaccgt gtggggtgaa
    20581 gtggctatgc ctggatatgg cctgtatgat cgtgcaatgg gactcactta catgcaaggt
    20641 ctatatgagg cgataagtaa ggaaaaacca ctgggagagc attatgaata aaatcaagct
    20701 cttcatcttt gatatggatg gccttttatt agaaactgga cgtttagctt atcgtgctta
    20761 tgtaaaatca gcacaaaagt atgattatga aatgcgtaag gaagtctatt atttactaac
    20821 tggccaaaca gaaatggcga taagagaaca aatgggcatt ttgtacggag aagatgttcc
    20881 atatatcaaa tggcgagaag caatcaatca atataaagaa aaaatcgtga aagaagataa
    20941 gagagtatat acgaaaaaag gagcagaaga aattttatct tttgcaaaag aaagaggaat
    21001 ccatacaatc gtggcttcgt ctaatacacg ggtaaaagta gaaatgtatt taaaaatgga
    21061 aaatctatat gccttgtttg atcaaatcat ttcaggggat gatgtaaaaa aaggaaagcc
    21121 ggaaccagag atctttttga aagcttgttc aaaaatgaat attcctccaa gtgaagcact
    21181 tgtatttgaa gactcgattg cagggatcga agcagcaagg cgagcgggca ttttgtcttt
    21241 tctcatcaaa gatcatttga tagggttgcc cgatcataat gggaagcata agttgaaagt
    21301 agatgtaagt ttatatgaat ccaataaagc agactatgaa ttttatgacc tgcatcaagc
    21361 aaaacgattt ttagaaagta aagggttgta tctctaataa agaggactat tttctagtcc
    21421 tctttattta ttgtcctgca cgactgaggg aacagatgaa aaagaagtaa ttttttattt
    21481 tgaagtatct accttatttg tagtgaacaa tttagaacaa acacaaaacg catctactgt
    21541 tatctaccag tagatgcgtt taaagagaag aacgagaatc gtccatatga gggtttccac
    21601 tccttatgca gattttttta attcttgcac tgcttgttcg atcgtaacac ctgtcgctac
    21661 atgattggta ttttctttgt caaaaacgac aaatagattc aaatctgtat ccaaggttac
    21721 tcgatattgt atatctttgt tatagaatgt catagccatt ctcctttctt taaatacttg
    21781 ctgaaaggca acgaacgcgt tgataacgaa attccattta taagtatacc accaattgaa
    21841 aggttttcat agttaagaaa ttttaaagtc aaaagttgca gataaaagca aataaacaag
    21901 aaaacaaaaa gtgaatcaat cgcggttatt caactttttc ttcgattcga agacaataga
    21961 agacaatgaa ttttatattt cattgcatta tccttgtgtt attagaaaaa gcatgatata
    22021 ttaaatgagt aatctatttt gaaacgtaat ttgagcgata tatctacata gataaaactc
    22081 cttttgccta tcaaatattt attgcaacga aaaaacgtac attgatacgt atcaggagga
    22141 atttatttat gaataacggt acagtaaaat ggttcaacgc agacaaaggt tttggcttta
    22201 tcactggtga agatggaaat gatgtatttg cacatttttc agctatccaa ggtgacggct
    22261 tcaaaacttt agaagaaggc caagcagtgt cttacgatgt tgaagatggt caacgtggcc
    22321 ctcaagcaac aaacattgtt aaagcttaat aaaaattgac ttttaaccgt cttttcgaaa
    22381 gaaaagacgg tttttttgta cgaaaaaatt
//
LOCUS       DACDYJ010000044        21845 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDYJ010000044 DACDYJ010000000
VERSION     DACDYJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.044"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(25..1521)
                     /gene="lysS"
                     /locus_tag="IWM51_001756"
     CDS             complement(25..1521)
                     /gene="lysS"
                     /locus_tag="IWM51_001756"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP9971178.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="IWM51_001757"
     CDS             complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="IWM51_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP9971179.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="IWM51_001758"
     CDS             complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="IWM51_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP9971180.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3741..5852)
                     /gene="hflB"
                     /locus_tag="IWM51_001759"
     CDS             complement(3741..5852)
                     /gene="hflB"
                     /locus_tag="IWM51_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP9971181.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="IWM51_001760"
     CDS             complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="IWM51_001760"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP9971182.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="IWM51_001761"
     CDS             complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="IWM51_001761"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP9971183.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8052..8522)
                     /locus_tag="IWM51_001762"
     CDS             complement(8052..8522)
                     /locus_tag="IWM51_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP9971184.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8568..8993)
                     /locus_tag="IWM51_001763"
     CDS             complement(8568..8993)
                     /locus_tag="IWM51_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP9971185.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9060..9329)
                     /locus_tag="IWM51_001764"
     CDS             complement(9060..9329)
                     /locus_tag="IWM51_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP9971186.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9340..10935)
                     /locus_tag="IWM51_001765"
     CDS             complement(9340..10935)
                     /locus_tag="IWM51_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9971187.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="IWM51_001766"
     CDS             complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="IWM51_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP9971188.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14488..15048)
                     /locus_tag="IWM51_001767"
     CDS             complement(14488..15048)
                     /locus_tag="IWM51_001767"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP9971189.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15401..16375
                     /locus_tag="IWM51_001768"
     CDS             15401..16375
                     /locus_tag="IWM51_001768"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9971190.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16545..17945
                     /locus_tag="IWM51_001769"
     CDS             16545..17945
                     /locus_tag="IWM51_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP9971191.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18023..18745)
                     /locus_tag="IWM51_001770"
     CDS             complement(18023..18745)
                     /locus_tag="IWM51_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9971192.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18824..19330)
                     /locus_tag="IWM51_001771"
     CDS             complement(18824..19330)
                     /locus_tag="IWM51_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9971193.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19453..21252
                     /locus_tag="IWM51_001772"
     CDS             19453..21252
                     /locus_tag="IWM51_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP9971194.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21320..>21845)
                     /locus_tag="IWM51_001773"
     CDS             complement(21320..>21845)
                     /locus_tag="IWM51_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP9971195.1"
                     /translation="PESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQ
                     PKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRD
                     IGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKV
                     GSVQWFAPDFAVSM"
BASE COUNT         6051 a         4791 c         3968 g         7035 t
ORIGIN      
        1 attccttaga cagcttttat tcgattatct cattgttggg aatagcaaaa catcccgaat
       61 tgattgtgca tcagttagca acattactaa tctgtcgatc ccgatgccta gaccaccagt
      121 tggcggcata ccgtattcta aggcttccaa gaagtcttcg tctacaccgt gagcttcgtc
      181 atttcctagt tcacgttctt tttcttgcgc ttcgaatctt tctctttgat caattggatc
      241 gttcagttct gtaaaggcat tagcaaattc gcgccccact atgaatagtt cgaatcgatc
      301 tgtgaatcga ggatcttctg gatttttctt cgctaaaggc gaaacttcga ctggatgtcc
      361 gtatacaaac gtcggttgtg tcaatgtttc ttcaacaaat gtttcaaaga attcattgat
      421 aacatgtccg acacccatag cttcggttac ttctacattg tgtttttcgg ctaaagctaa
      481 tgcttcttcc aatgtcattt cttgccagaa gtctacacca gtttgttctt tgattgcatc
      541 taccatatgg atgcgtttaa agtcactttc taagtcgaca gcttgtccat catatgtgat
      601 ttttgctgtt cctagtactt tttcagctgc gttacggata ataccttctg tcagattcat
      661 tacatcattg aaatcagtat atgctgtgta tgcttctaac atcgtaaatt ctggattatg
      721 tgtagtatca attccttcat tacggaaaac gcggccgatt tcataaactt tttccattcc
      781 gccaacaatt agtcgtttta aatgaagttc tagcgcaata cgcaaatata aatcaatgtc
      841 cagtgcatta tgatgcgtga taaatgggcg tgcggcagct ccacctgcct cattgtgcaa
      901 tactggtgtt tctacttcaa tatagccatt gccatctaaa tagcgacgga tttcgctgat
      961 aatttggctt cgtttcataa atcggtcaaa gctttcgcga ttactgatta aatccaagta
     1021 gcgttgtctg tagcgttgtt caatattcgt taagccatgg tatttatctg gcagtggacg
     1081 aagtgctttt gacaaaatcg tgatttctga agctttgata gaaacttcgc ctgtgtctgt
     1141 tttcatgatt tgtccagtta caccaaagaa atctccaagg tctgcatgtt tgaatacttc
     1201 ataggcctca tcacctactt ggtcttttct tacataaatt tgaatttggc cttcacgatc
     1261 ttgtagatgc gcaaatccag ttttcccttt tcctcggcga gtcatcatac gaccagctac
     1321 actagcggtc aatcccattt ctgctagttc ttcttttgtt cttggatcaa atagttcatg
     1381 caattcttct gagttgtgcg tgcgttcaaa tcgttttcca aatggatcga ttccatcttc
     1441 acgcaattgt tccatttttt gacgacgtac aagcatttga tcatttaaat cttctacttg
     1501 atgttgttgt tcctgtgtca caaaagttcc tccattctca tgttaatctc tttcctataa
     1561 tgccaatgaa acacaaaaaa agcaagccga acttggaatt tcggcttgaa aattcacgaa
     1621 aaacttagaa atcacaacgt ttgtttcaat agtacttttt ccgctttttc tacaaattga
     1681 tccagcaggt cattgatttc ttgctgtttt tctgtctgat tgattgctac tttcactttc
     1741 gctgctcgag gaatcccttt taaataataa gccgcatgtg ttctgaattc tctacaagct
     1801 actttttctc ctttgagatc tgctaagcgt tgtaaatgaa gtttagctgt agcaattttt
     1861 tcggctggtg ttggttcagg aatcagttct cctgtttcga gataatgctg tgtgcggtga
     1921 atcatccatg gattgcccag cgctgcacga ccaatcatca caccgtctgc accgacatag
     1981 tcaagcatcc attttgcatc ttctggcgtt ttgacatccc cgttccccat aaatgggatc
     2041 gtcaaatgtt ttttcacttc tttcaatact tcccagtccg cttttccttc atacatctga
     2101 acccgtgtac gtccatgcat agcgatggca cttgctccag ctgattgtgc tgctaatgca
     2161 ttttctacag caaagacgtg ctcttcatcc caaccaatac gcatcttgat tgttactggg
     2221 atatttactg ctgaagaaac agcttggacc atttcgtata ctttctcagg atctaaaagc
     2281 cattttgctc ctgcttctgc tttgattact ttatttactg gacagcccat attgatatct
     2341 attatatcgg cagctgtgtt ttcttcgaca aattgagcag cttcaactag actatcttta
     2401 tttccgccaa atatctgtaa acttaatgga tgttctgttt cttcaatgtg gagcatctct
     2461 aaagtttttt tgtttctgaa atgaatacct tggtcactga tcatttcaca tacgactaag
     2521 cccgcaccaa attctttgac agtgactcga aatgctgaat ttgttattcc agccatcggt
     2581 gcaacaacga cacgattagg gatttccaca ttgccaattt tccatgtgtg ttccacttaa
     2641 atccctccta aggcttcttt cttcaatcca tctaattctt cttcgctata cttgtatttg
     2701 ttattacaga aagaacaaac cgcttctgca ccgtgatctt cgtctatcat tgcttggatt
     2761 tcttctgcac ctaacgcgat aattgcttct gcaaattttt cttttgaaca gtcacatttg
     2821 aattgaacag gcattttttc caaaatgttc aattcttctg taccaagcaa tcctttcaat
     2881 atttcttctg gtgtctgtcc ttcatctaag agggtagaga ttcttggtgt cgtttttaat
     2941 tgttcttcga tcttgctgat tgtttcttca tcggcacctg gcatgatttg gatcatgaat
     3001 cctcccgccg tcttgatgct gtcgtcagta ttgaccaata cactcagtcc aacagctgaa
     3061 gggatttgtt ctgagatagc aagatagtaa gtaaaatctt cagcgatttc tccagaaaca
     3121 atcggcgttt gcccagaaaa aggttctttt aatcctaagt cttttgtgac agtaaacatt
     3181 ccttgtgttc caactgctcc ccgcacatcg attttacctt cactatttag aggaagactg
     3241 agatgcggat ttttgatgta acctttcaca tctccctttc catttgcatc tactacaatt
     3301 cctgttgctg gaccatcgcc ttggattcga acagttagtt tgtcttctcc tttgagcgtc
     3361 gcaccaagta gcagactacc gaccaatgtc cgtcccaaag cagctgagga ggtgtgccaa
     3421 gtatcatgac gtctttgtgc ctccgctacg gtatttgttg cttgaacggc gtatgctcgg
     3481 acgaatccgt cgtgagcaag tgccttaacc aaataatctt tcatttgtta tgtactcctt
     3541 tttccgtttt taaactcata gtgaatcata ctagcaacta ctgctttttt caactttgga
     3601 atgcttcttt taaaaataat tcagaatcat ctagaaaaca ggacatcacc ctatttacaa
     3661 cttaaataaa gaaatctccc gaaaaccggt ttttgttccg gttttcggga gtcatatcag
     3721 atcaattgac ctatattttt ttatttgtat tgatcgtctg aatgattatc ttctgattgt
     3781 tgagaatctg aatcatgttc tgctttattt tcctcattca gttcatgttt tgcttcttca
     3841 aaatcacgtt tttcttccgc ttgtttttca gcgtcttttt cttccaaagc acgttttgct
     3901 tcttcaaaag ttttcgcttc tttttcgctt ggataatcat ttgactcagt accttcaggc
     3961 atcacaccgt gttcaaacaa tgacttgatt gctttggcat ccaatgtttc gaattccaaa
     4021 agtttttcag caatcagttt atgctgtgca cgatgagctt cgatgatttc tcgagcttta
     4081 tcatgagctt ccatcaagat ttttcgtact tcttgatcaa tctcgaaagc gacttgttcc
     4141 gagtaagctt ttgtttgacc atagtcgcgg ccaacaaaca cttgatggtt tccttcatat
     4201 tgaacaggac ctaaacgatc actcattcca tattctgtta ccatgcttcg tgctagagct
     4261 gtcgcttgct caaagtcatt ggaagcacca gttgattgga cattgaagat gatttcttca
     4321 gctgtacgtc caccaagtaa gcctacgatt tgttcaaaca tatcttcttt cgtcatcaag
     4381 aactgatctt cttttggtaa agcgatcata tatccgccgg cgcgtccgcg agggatgatc
     4441 gttactttgt ggacgacccg agcgcgactc aatactaatc cgacaatcgt atgaccagct
     4501 tcgtgataag caaccatttc gcgttctttt ttgctgatta cgcgatcttt tttagctgga
     4561 cctgcaatca cgcgatcttc cgcttcatcg atatcagaag catcgatttt ctttttattt
     4621 cttctagcag ctactaacgc tgcttcattc aagacatttt ctaaatctgc tccggcaaaa
     4681 cctggtgtct gttgagccac aactttcaag tcaacgtcat ctgccatcgg tttattccgt
     4741 gcatggactc gcaagattgc ttcacgacct tttacatctg gacgtccaac taaaatctgg
     4801 cggtcaaaac ggcctggacg tagtaacgct gggtctagta catcagaacg gttggttgca
     4861 gcaatgacga ttactccttc atttccgtcg aagccatcca tttctacaag caattggttc
     4921 aatgtttgtt cgcgttcatc atgtccgccg cccataccgg caccacgttg acgacctacc
     4981 gcatcaattt catcaatgaa aatgatagct ggtgcgtttt tcttcgctgt ttcaaacaaa
     5041 tcacgaacac gacttgctcc aacaccgaca aacatttcta cgaaatcaga acccgagatc
     5101 gagtaaaacg gtactccagc ttctccggca acagcttttg ctaataaggt tttacctgtt
     5161 cctggaggtc cttctaaaag tacacctgcc gggatacggg cacctaattc aacaaatcgg
     5221 cgaggatctt tcagaaattc aacaacttca actagttctt gtttttcttc ttccgcaccc
     5281 gctacatcag aaaaacgtac gcgattagct tttttgtctg cttctttggc ttttgattta
     5341 ccaaagttca tgacacggcc gccaccgcca ccgccgccgc cttgttgtcc catcatcatg
     5401 taaaagaaaa agatgatgat tacgattggc aagaagctaa gcaataggga aacccatata
     5461 ccgctgcttg attcttcttt tactgttgta gcaactttgt tttcttttgc cagttctgtt
     5521 acttggctca atgttgtatc atttggcaaa atgattgttg tgaaatgggt agtagtcgta
     5581 tctgttgaac ctaagatagc taacccaccg ctgttagcga cggtctgctg ttctttgtat
     5641 tcaccagtga tcttatatac accatttgca ggctgcactt caaattcttt gatcttgcct
     5701 gcttgtagtt gttctgtgaa ttttgagtac tcgatatctg acgatcgctg gccgttatta
     5761 ccaaaaatga aataaacgac cattaccatc gctaagatta cgaggacata atacaaggca
     5821 tttttcatca tgccgttgtt ctttttgttc atagttgccc tccctaagcg tcaatttaat
     5881 ttaacagttt tttatcaatc ctgaccattc tattgtacca taatttaatt gagatacagc
     5941 attaatttga ttgataaaca ctaggtttca atacaccgat gtacggaagg ttacgatatg
     6001 cttctgcata atccaagcca taaccgacca caaattcatt tggtacgttg aagccaacat
     6061 aatctgcttc gatatttact actcttcctt caggtttgtc aagtagtgta acaattttga
     6121 ctgattttgc tttgcggtat ttgaaaagat ccactaaata agcaagtgtt cttccgctgt
     6181 caatgatatc ttcaacgatc aacagatcgc gaccttctac attcgtatct aagtctttga
     6241 cgatttttac ttcccctgac gagactgttg cattcccgta gcttgaaaca tccatgaaat
     6301 ccaattccaa atacgtatcg atagaacgaa cgatatctgc catgaatggt accgctcctt
     6361 ttaagacacc tactactagg ggattggtgt tttggtaaat ttctgttaac tctttgccta
     6421 gttctttgca acgaacttgt atctcttctt gtgagatcaa aattctctca atatcctttt
     6481 ccaacatcag gttctccttc caattgcttc tttttgatat ttgtaaagaa gtatgtagtg
     6541 tattttatca gtttcttcac gaatactcaa atatgaaaag gcaaaaggta tcaagctata
     6601 tatatttctt ttggcatctg taatgatcca agcatgctgc ctttgtgaat taggtacttt
     6661 ctgatcgata aaatatctag atactttttt tgtgagattc tctgttaatt taatgcggtc
     6721 tcctgctttt cttttttcga catacagttg cacatcagca ggaatccaaa tgtcatgaga
     6781 aaatagagac catgaatctg ttgctttgat gtcccctgca ttttcaggtt tgaccagacc
     6841 aatccattct gtttcattca aatatattcc ttgttctgga atcagcaaca agcgctggtc
     6901 ttctgtctgg ctagttgctt gtttttcttt tttagagagt acaacaaaat gatactctct
     6961 tttcagtatc caattattgc caatactgag ttgccattgg ctttttttct gttgccactg
     7021 gtctaaaatc gactccattt gttgttcttt caaaataatt cctgtttttg agaatgcttt
     7081 gtcgaaaaag gagtaaaaaa aatagtatcg ttcagcatct gtcatttctt caatcatttc
     7141 tgcagaaaat cgaaattgtt cctcccactg ttcgacttcc ttttcgatca actgtcccat
     7201 atatttttga atgacttggt ctgcccattg gatctgttgc gaaaattgct ggaaatgtct
     7261 caacgcctgt gtattttctt gtttcaattg aggaactacc aaatgacgaa gccgatttct
     7321 ctggatatct aacaattggt ttgtctgatc ctcaaagtaa acaagctgcg attccgctgc
     7381 ataagtatat aggtcttctt tgctaaaact cagcaacgga cgaatcagtc tgcccgtagc
     7441 aaatggttgt acttctttga ttccagaaaa agtatttaac tgtcccccac gaacgatttt
     7501 cattaacacc gtctcgattt gatcatctcc gtgatgagca gtcatcaacg tgtcatattg
     7561 tccttcttcc atcacgcgag tgaattcttg ataacgaaag ttacgagctt ctgtttctat
     7621 ggcggatttg gctggatctt cccaaatact tacatataaa gggagattct tctcttcaca
     7681 atatgagcga agataggcag cttcttctgc tgattcttct cttaattggt gattgatgtg
     7741 gacgatacct aaagaaaaac cgatttggcg ttgcacttgt aaaagtaaat ggagcaaaac
     7801 catcgaatca acaccacctg ataccgctgc taaaattctc gatccttgtt gccagaatcc
     7861 ttgttcattc ccgtgccgaa taaatgactg ctgtaatagc ggtgcttttt tcccgccttt
     7921 ttcaacgttc atcctttcca caccctttct tcctaaaaaa aagagagcgg gaaaacgtct
     7981 aaaacgacaa ttaacctgcc ctctgttgat actatgaccc aaatagtcgc actatcatta
     8041 tttgcggctt tttagctgcg acgtccgcca cggccaccgc gctttccttc tgtgtttcgc
     8101 ttcaatgaag ataagcggtc atcgctatct ttcaaaaatg aactcatcaa tgaatcgaag
     8161 tcttcttttt gagctgaagg ttgactgcga gaaaaagatt ttttctgtgc tggacgattt
     8221 cggttgtcac ggggatcttt ttgttgatat tttttaggac ggctttcttc tccagattca
     8281 gcaggttgtt cctgcgcttt ccggatagat agtccgattt ttccatcatc atttactgaa
     8341 ataactttaa ctgttacttc atctcctaca cttaaaacat catggatgtc ttttacatag
     8401 ccatttgaca cttcgctaat atgaactaga cccgtttttc cagcacctag atcaataaac
     8461 gcaccaaaat ttgtgattcc tgatactttt ccttgcagtt ttgctcctac ctcgattgac
     8521 ataaaaaaaa tgttcctcct aattatttcc cttttttata ttcctgctta ttttgcagtt
     8581 gtagaactag ttgattgatc tgtttcattt gtcgagctgt ctgtatttga ttctaatata
     8641 ggataaactt tttcaccgtc ttttgagtaa aagaatttgc ttctggcaag tttagctaca
     8701 tactcatcat ctttcagcaa tgaaacatct tggtgcagct gatcaacgtc tgcgttgact
     8761 tctttcattt cagtatttgc ttcgactctc aattcgttca attgatgcat cctttgcatg
     8821 tcatggaaaa tctgcacacc tattatcgcg aaaatgacca aagcaccaac aaaaattgca
     8881 gccagtctgc gtcttcgaaa aatcagttga cgttgttgtt gctgaaattc agcatacttt
     8941 ttctttgcat attccgtatc tagcgcagcg attttattgc tttttttcgt cacttggatt
     9001 cgctccaatc tttttgattg tctttttcat tataccaata tcgtgaaagc ttgtctaatc
     9061 tatttctcta gtgtttctgt gcgattttca gaaataattt cgtacatttt ttgtgcatct
     9121 tcttttttgg ttgattcatg taattccgta attttcactt ccaacgtttt gttgccaaac
     9181 tggactttca aaatatcgcc tactttgaca tcactggaag atttcgccaa tttaccgttg
     9241 atttgaattc ttcctttatc agctacttct tttgctactg atcgtctttt gatgattcgg
     9301 gaaattttta aaaatttatc taatcgcata tcttattcct cactttttaa tttttttatt
     9361 taatcgcagg attttcttac caaaaggcaa cattagccat tctcgtactg taaacagacg
     9421 aagacgaatc actgcataaa tgaacacaga agccccgatc ccaactccta tcacgctgac
     9481 aaaaaaagca gtggaacgat gagttacagg accaaatact atattcaata caccataata
     9541 cagcagcagt actactgtca tacaaagaag gctgaacatc aacttccgtc caaaatgccg
     9601 ttctttccaa aaagcattta gctcttttgt ttcaattcga acaaaatacc aaagcgtcac
     9661 acctaatcct aaaagggtag acaggcttgc acctgctgtt cctaataaac cggtcagaaa
     9721 agaagtagtc aatcctttta ccaatagtcc ccatcctgcg ccttttaaag aagggcgaaa
     9781 cgagttcttg ctttgagcaa tactttgata agcttggatg actgcagtga aagcaatgga
     9841 aaaaacaaat aatacaagcg ctgcatttcc cgcataatct ttaaacaacg cataattgat
     9901 ataagggagc agcaaggcta atcccaaaga cgcagctaat gctaaagcgg tcgtcaatct
     9961 aagatagatt ttggctgtct gtaaaaactg acgatagatc cggttggtca aatgacgagt
    10021 caacgcaggc aggaatgtcg cacttaatgc agtggcaatc actaaaccta attgtactaa
    10081 aggctgacct cgatcataga ccccttttgc tatcttcgca ccgtattctg acagtccgta
    10141 aacttccaaa gcatttttta caaagaaaga gtcgatcagc tggaaaaaga taagcaatcc
    10201 actataaatc gaaaccagtc cgccttctac cagaaaacgc cggatcaagg gctttgacgg
    10261 ctgtttgaac aatggaaacc gtcggaagct taacgctcct ccatgaatct tttgttcata
    10321 gtagtgaagg atcccatatg cgcataaacc gccaaaaaca gctccactca ttgctgccgt
    10381 ccctgtttgg tacaccgtcc atccaagctt tctaaaagca aacgcactac agacaatcac
    10441 aaaaacacga acgaactgct caactacttg agaaacagca gttggttcca tcaagaatct
    10501 tccttggaaa ttccctcgat aaaaagatag accaggcatc agcaaaaagg taaaggaaac
    10561 tacttggatc agcggtttca attgtatgtc tcccatcatc caagcaatcc agctgctaaa
    10621 tgcaaagaca aacaaccaca aaaagacacc tgtccaaaat actaagggat aaagattttg
    10681 caaagcttgc tttcgctcga aaggattctt tctttctgca acgtactttg atatgaactg
    10741 cggaagaccg gataaagcta aagtcattgc tatcccataa attggataaa cttgctgata
    10801 aacataaaaa ccttcgtcgc cgactaagtt ttggagggga attcggtaaa gggcacttaa
    10861 tacttttgct atgaaagaag caatcgtcaa tacaaatgct ccttgcatga cgcgccgcat
    10921 ttctttatga tccaaatcat tgctccttcc tacgctgaca gatatttttg ctcacgcaac
    10981 gctttgacaa acttttggat ttcctgaagc catactgctt cttccatttg gttggggatc
    11041 gtcaagcgta tcaccatctg ttctttttca acgcctagac tggcttttag ttttgtcgct
    11101 gtcagtgctt cgaataactg ttccacacta tagcgttttg ttccaacttt acttagagta
    11161 aagactatat gcgcctcctg tttccttatc gtttctagga gcgctcgatc gccatccatt
    11221 ttgatctgcc caattgtcaa gagatgtgct acttcttctg gatattctcc aaaacggtcg
    11281 agcagatcat cttctagttc atctagcatt tcttgcgaat caagttcacg aattcgttta
    11341 tagatctcga ttttctgccg ttgatctgcc acgtatgttt caggtagata cgcatcgaca
    11401 ccgaggtcga tctcaacagt ggtcttctcc acttgactat tctttccttg tttacgagaa
    11461 acagcttcgc ttagcatttg agaatacata tcgaaaccga ctgcatcaat aaatccatgc
    11521 tgctgagcac ctaagagatt acctgcaccg cgaatggaaa gatcccgcat cgcaatcttg
    11581 aatccagatc ccagttctgt aaaatctttg attgcctgaa gacgtttttc actgacttcg
    11641 ttaaggatct tctgctgctc atacataaag taagcatatg cgacacggct gcttcttcct
    11701 acccgccctc gcaattggta taacgtggat aatcccatat aatcagcgtt ctcaacgaac
    11761 aacgtatttg cgttcgggat atctactcct gtttcaatga tggttgtagt gacaagaaca
    11821 tcatattgac cttcaataaa ttcaaaaagc gtattttcta attgcgcctc tgtcatttgc
    11881 ccatgagcat aaccgatccg tgcttccgga acgagttctt ggatttcttc tactttttgt
    11941 tcgatcgttt ctacccgatt atataaatag aatacttgtc cacctcgtcc catttctcga
    12001 tggattgctt cgcgaatagc cccaggattt ttttccataa catacgtttg gatcggataa
    12061 cgattcgcag gcggtgtttc gattactgaa agatcccgca cgcctaacat cgacatatgc
    12121 aaagttctcg gaatcggtgt tgcagtcaga gtcaaaacat ccacttgtgc acgtaactgt
    12181 ttcaatcgtt ccttatgttt tacaccaaaa cgttgttctt cgtcaatgat cagtagaccc
    12241 aaatcgctaa attcaatatc ttttgacaaa attcggtggg ttcctacaac aatatcaact
    12301 tgacctgtac gcagttgttc gatggtttct ttttgttgct ttcttgttct gaaacgactt
    12361 aatagcccga tatttacggg gaaaccttca aatctttcca gcatcgtttc atagtgctgc
    12421 tgtgccaaga tcgttgttgg aactaaaaag gctacttgtt tgctttcctt tatcgcttta
    12481 aaggccgctc gaagcgctac ttctgttttc ccataaccaa catcacctac gagtagtcga
    12541 tccattggtt tttctttttc catatcccgt ttgatctccg ctgcacttct caactggtca
    12601 tcagtttcag agtaggggaa ggcattttcg aattcttttt ggtaggcgtc atcaggtcca
    12661 aaagcatatc ctttttctga ctctcttgcg gcatataatt tgattagatc gtcagcgata
    12721 tcctcgattt ttgaagagac ttttcgcttg gttttcgtcc attcacttcc gcctagctta
    12781 ttgatgcgag gcgctttggc ttcagaggcg acatattttt ggatcagatt cagttgagta
    12841 acaggaataa acagtttgtc gtcgttttga tacaaaatag tcatatagtc ttggtgaacg
    12901 ccatctactt ctagcgtttc catcccaatg tatttcccga taccatgatt ggcatggaca
    12961 acgtaatctc ctgcttttaa ctcattatag cttttcaatc gctctgcgtt tgttactgtt
    13021 tgacgtctcg tttgtttttt cgtcgttttt tggaaaattt ctttttctgt gatagctact
    13081 attttttctt gaggaagttc aaaaccagcc tgcaaagctc cttcaactaa ttgtacctgt
    13141 cctctgatca atgcattttt ttctgtttct actactaata tgtcttcatc tcgaagcatt
    13201 tgttctgctt tgcgtattct gtcctttgtc ggaataaaga tcacaacagt ctgtttttgc
    13261 ttgcgccaac ggtccatttc tgttttcagc aatggcattt gtccgaagaa ctgttgcatc
    13321 gtccgatatt gaaaattatg gattgcttgg aaacgaagat ttcccatccc tttttggaag
    13381 agtgagaaaa aggttgtggt gaacttttcc ttctgcacga ttttttgtat atctgctgaa
    13441 aatgtctgct cagaaaacac ccgcaattcg ctgatttttt gagtctgcca ttctgccgct
    13501 tccctttcga tttcccgatt tgtttccatg attcgctgat aatcatcaac gactagtaaa
    13561 ctatttgaag ggaaataatc caagatcgtc acagattctt ggtaaagaag atctgagtag
    13621 aattttgctt cttctgtcgg gacacctgct tgccagcttg aagtcagctg accgaagtaa
    13681 tcttgcaaaa actcgcggtc tgcttcttct gtggtcgcag caagccgttt ttctagcatt
    13741 ttttgcattt gctctattcc atgttctaga tcctctggtc cgtagacttg ttcactcgtt
    13801 ggggaaaccc agatttcttc cgttttttcg attgagcgtt gtgtttctgc ttcaaaatac
    13861 cgcatagaat ctatctctac atcaaatagt tctacacgaa caggataggt tgtcgttaac
    13921 gggtaaatat cgataatgct tcctcgaata ctgaattctc ccggttttgc gaccatctct
    13981 tctcgtacat agcccatcaa agtcaattgg cgaggaagat tttccacgtc tatctcatca
    14041 ccaatatgcc aattcaattg cgcctgtagc caagttttct tattcggcag tcgtttgtgc
    14101 aaagatgcga ctggcaacaa atagatacct gctttttttt cttgaagtgc gttaagcgtt
    14161 gcaactctct cgcctcgcgc ttctggagaa gaaaaggcca tttcagcaga aagcacttca
    14221 tccactggaa ataaatggat ctgatcttgc ggaatcacat gttgcagatc ttctgctaac
    14281 tgattcgcat aatacaaatt tggcatcaga ataatcatgt ttttatttgt tttcttaaac
    14341 gctcgagcaa atagaagtgt tttcgctgag cccgctaatc ctgtgattag ttgtctaggc
    14401 attttttctt ctaaattgct gacccattct tttacttgcg gatcttttcc gactaattga
    14461 ataatatcca ttccttttcc tccttcctca attgaatcga ttcatcgcat ccacgaacga
    14521 atgcccttct atgagatatt cgactgcttt aacagattga cacatgcttt cttctatcaa
    14581 aggctgattt tctttgctga aaggactaag cacatgctgg actactgttt ttttcccttc
    14641 tggcctgccg attcccacct tgattcgatc aaagacattc gtattcaaat gggaaataat
    14701 acttttgata ccgttatgcc cgccggcact tcccttttga cggagccgga tttttcccac
    14761 tgccagatcc aaatcatcgt agattacgac taattcctct ggataaatcc caaaataagt
    14821 cattagtggg ccgactgcac gaccagattc attcatgaat gtttgtggtt tgactaaaag
    14881 gatcttttct ccattatgaa aaaattcggc aacttctgct tcgaacggat tttttttgaa
    14941 agtagcttga tgctcgcgtg caattttatc cacgaccata aagccgatat tgtgttttgt
    15001 atattggtat ttggtacctg ggtttcctaa cccgacgatc attttcattt ttgttttcca
    15061 cctaattcta aaaagcagca caaaaggctg gactgctaat tagcggtcca gtccatcgag
    15121 cttttttctt ttttatactt gtcaatgcat agtataacac aaaacaaggc gtttcggcta
    15181 aaagagaatc gttaataaat gataaagaaa ttaaaaattt cttcaaagtt taatgaaata
    15241 attgatatat aaaaatagtg aaataacgca catgtaagct tttacatata atacaacctt
    15301 gttatctgtt atattttata gccaaatagc gtgacatggg gcttgagaaa tgttagcata
    15361 agcatgggaa aaaacaaatc aatggaagga ttgatactaa atgactgcag ctgcagaaaa
    15421 aaaagatcat caaaaagtta ttcttgttgg agacggcgcc gtaggttcta gctatgcctt
    15481 tgctctagtt actcaaaata tcgctcaaga agtaggtatt gtggatatca atactgctaa
    15541 aactgaagga gacgcaattg acttgtctca cgctttggca tttacttcac ctaaaaaaat
    15601 ctattctgct acttacgcag acgcacatga tgctgactta gttgttatta cagctggcgc
    15661 accacaaaaa ccaggtgaaa ctcgtttaga cttagtaaac aaaaacctaa aaatcaaccg
    15721 cgatgttgtc acacaaatcg ttgaatcagg atttaacggt atctttttag ttgccgctaa
    15781 cccagtagat attttgactt actcaacttg gaaattctca ggtttcccta aagaacgcgt
    15841 aatcggttca ggtacctctc ttgactcagc tcgtttccgt caagcacttg cggaattagt
    15901 tgacgttgat gcacgtaacg tccatgctta catcttaggt gaacacggag attcagaatt
    15961 cccagtttgg tcacatgcaa atgttgccgg cttgcaaatc tatgaatggg taaaaaacaa
    16021 cccagaaatc gatgaagaag ctatggttaa cctattcttt ggcgtacgtg acgctgctta
    16081 cacaattatc gagaaaaaag gcgctacttt ctatggtatc gcagtagcat tagcacgtat
    16141 cactcgtgcc atcttagatg atgaaaatgc tgttcttcct ttatcagtat tcatgaatgg
    16201 cgaatatgga ttgaacgaca tctacattgg tgcacctgct gttatcaacc gtcaaggtat
    16261 ccaaaaagta atcgaaattc ctttaagtga cagcgagcaa gaccgtatgg ctgcttctgc
    16321 taagcaatta aaagaaattc tagatgaagc ttttgctaaa ctagacgctg aataatcttt
    16381 gaacatgacg gaaaacatct attcggtgtt ttccgttttt ttattttatc acccttatgg
    16441 tttctaaatt ttttcataaa gtagagataa ccttcgtctt ttcctctaaa ttatgctatc
    16501 atgagaccgt tccaccctaa acttttactt aaggagttgt cgatatgaca agatcttcgc
    16561 atcgaaaaaa atcgaaacat gttgctttgc tttcagttct tggtgttctc ttagtgctga
    16621 tcagctttta tacctatcgc agtacatact acacaaagca ttttttgccg aatacagaaa
    16681 taaatggaat aaatgtcagc aatctaacag tcgaaaaagc aaatgaaaaa ttaaaagaag
    16741 cttattctga taaaaaactg gctattaaag aagatggaaa agtctggcag gaagtaccaa
    16801 aatccgagct cggctacaaa gatgatttca cttcggagct ttctccgatc ctaaatgaac
    16861 aaaacggatg gacttgggga atgacttatg tctcagcggc agaaaaacaa gaaatagatc
    16921 ctgtcagtca aaaccaccaa aaactagaca ctaccgttca gacattgaca actaagctga
    16981 ctgatttgaa caaagaccgg acacctacta ctgatgcaac gatcgaaaaa gcaggtgact
    17041 ctttcgttat caagcctgaa gtcaatggaa atacgatcga cgtcgatgct gcggtcaaac
    17101 aattaaaaag tgcggtaaac tctggtaaag atacaattga attgactgaa ttcaaagaaa
    17161 aaccaaaagt aacatctgaa gacagctcac tgaaagaaca actcgcatcc atgaatgcta
    17221 tcgcaaatgt aaaagctact tacagtataa acggtgagac tttccagatc ccatcaagtg
    17281 atattatgag ttggctgacc tacaatgatg gaaaagtcga tttagataca gaacaagtac
    17341 gtcaatatgt aacagattta ggcacaaagt ataacacaag tacgaatgac acaaaattta
    17401 aaagtacaaa aagaggagaa gttacggtcc cagttggcac ttacagttgg acgatccaaa
    17461 cagacagtga gacagaagca ttgaaaaaag cgattctggc aggccaagat tttactcgtt
    17521 cacccattgt gcaaggtggc acaaccgctg atcatccatt gatcgaagat acgtatatcg
    17581 aagttgatct ggagaaccag catatgtggt attacaaaga cggaaaagtg gcccttgaaa
    17641 cagatatcgt ttccggaaag ccaactacac ctacacctgc tggagtcttc tatgtttgga
    17701 ataaagaaga agatgcaact ttaaaaggaa caaatgacga tggaacacct tacgagagtc
    17761 cggtaaacta ctggatgccg attgactgga caggtgttgg tatccacgat tcagactggc
    17821 aacctgaata cggtggagac ttatggaaaa cccgcggttc acacggctgc atcaacaccc
    17881 caccaagtgt aatgaaggaa ttattcggga tggtcgaaaa aggaacgcct gtattggtct
    17941 tctaacaaaa ataatatata aaaagaaagc acccgtacga ttggtacagg tgctttcttt
    18001 ttattatcta acaatgtgct acttacattt cagcagcagt aaagacttct gaaacatcat
    18061 catcgtcttc tagcttatct actagacgtt ccaattgctc tttttgttcc tcatttaaat
    18121 caatcgtcgt ttgtggaatc atcgtcaatt ctgcttgcgc caaagtgaac tctttttcca
    18181 actcatctcg tacagcagca aaatcttctg gtgcagtata aatctcgaat acttcaggag
    18241 aagtttgcag atcttctgcc cccgcctcta atacatcctc aaacatcgtc tcttcatcaa
    18301 cagtcaaccc atcgcgttca atagcgatat atcctttgcg gtcaaacaag taactgactg
    18361 aacccgtttc tcccagtgat ccaccgtttc gagtaaaagc tactcggaca tttgtcgctg
    18421 tccgattacg gttatctgtc aatgcatgga ctaagattcc tactccaccc ggaccatatc
    18481 cttcatacgt gatttcatcg taattcgttt catctacggc actcgtagcc tttttaatcg
    18541 cccgttccac attgtcgtta ggcatatttg cagcttttgc tttgtccaca acaagccgca
    18601 aagctggatt catagatgga tcaggaccgc ctgctttagc tgccatataa atttcacgag
    18661 aaagtttctg aaagactttc cctcgttttg catcttgggc attttttctt ccttggatat
    18721 tatgccattt actatgtcct gacatgattt ttcctccctt gttcttttga gaatacactt
    18781 ctattctagt actatttcac aaaaaaacaa agccacagcc aatctataaa cgcttcttga
    18841 atgccgcaaa aagcacacat aatatcacag cagtcagagc accgatcgaa tccaacatga
    18901 catcttggaa cataggtgtt cgtccaccgg tcatcatctg atggaattcg tccaaacctg
    18961 cgtaccctgt tgctgaaagc caagcaagga tcgctgtcag ccaccaaatt tttagcttgg
    19021 ggaaaacacc taaaaacaag ctgcctccca aaataaaata ggtaccgaaa tgtgcgccct
    19081 tccttaaaaa gaactctaaa aatttggcgt agcccagatg gtcgatactc accatacttc
    19141 caccataatt aaaacgtata cccgctaatg tgtctttaaa tggctgatta ggcagccagc
    19201 tttctaatcg cgaaacttgt gattgctgtc cgtaagtttg agaagaactg taaaataaga
    19261 ttataacgac tatcagtgcg attactaaat aaatatttcc gtttttcatt tgtttttttg
    19321 aagaattcat aagtcacctc tgtattttat cttagacttt ttttgaagaa acaacaatca
    19381 ttttcataga gacttcttcc ataaaaaagt gctatactta attttgtatt acaatatgaa
    19441 gggagttttt tcatgagtta ttcattaaat tggaatctag attctatttt ttccggaggc
    19501 agtcattctg atgctttgaa ccaacggatg aagcaattgg aagaccaaat gaacgagtat
    19561 tatcaccgcg taacaaaatg gtcacctagt tctgacaatg cagagcagtt aaacgctatt
    19621 cttcaattgc aagaaacgat tacgaatggt tttacccaat gcagcagcta tattactgca
    19681 ttgctttctg ccaatgtcaa tgattcagat gcaaaggtct tatcaggcaa gctttacgca
    19741 atgctgcctc gtctgcaatc agccgaaact gtattgtcaa agaaatttgc agaaatatct
    19801 gacaatgatt ggaatcagat gctttctcaa tcttcgtttg aaacaattgc ttttcgttta
    19861 aacgagattc gtcgtgatgg ctcacaatta ttatcagaag ctgaagaaaa cattataaat
    19921 acgctttctt tagatggtct aaatgcttgg agcagtcatt atgatacgat tgtagccagc
    19981 atttcgattc ccttcgaaca agatggacaa gtagtcgaat tatctgctgg acaagccttc
    20041 aataagatga tgggcgatcc agatccaaaa gtcagagaaa cacttttcgc agcatgggaa
    20101 gaagcctgga aagaaaaggc acccctcttc acggatactt tgaatcattt agatggtttt
    20161 agactttctg actatgaact tcatggtgtc acagattttc tacaaaaacc attagagtac
    20221 aatcggttga agaaagaaac attgtctgtc atgtgggata caatccagaa aaacaaacaa
    20281 ccccttgtta attacttgac tagaaaagct aatcttttcg gtaaagaaaa aatggaatgg
    20341 caagatcaag atgcacctat tattttaggc gatttgaaag aaaaaacctt tagttttgac
    20401 gaagcagcag catttattat cgaaaacttt aacaaattca gtccaaaaat ggctaaattt
    20461 gcccaatctg cttttgaaaa gagctggatc gaagcagaag atcgtcctgg gaaacgtcct
    20521 ggtggttatt gtactgagtt accagaaaca aaagaatcaa ggatctttat gacctacagt
    20581 aattctgtta atgaggttgc gacacttgct catgaactag gacatgcttt ccatagcagc
    20641 acgatgtggg atttaccttc tttaaatcgc gaatatgcca tgaatgtcgc agaaacagca
    20701 agtacattcg cagaactgat cgtagccgat gctacattaa aagtcgcaaa aacaaaagaa
    20761 gaaaaaatca atttgctaga tacaaaactg caaaatgctt tagctatgtt catgaatatc
    20821 cattcccgat tcatttttga aaatcgattc tatgaagcac ggcaagaagg attagtctca
    20881 gaagataaga tcacagagat gatggttgag gcgcaaaaag aaggctatca aggagcttta
    20941 gctacttacc atccatattt ctgggctgca aaattgcatt tctttattga tgacgtaccg
    21001 ttttataact tcccatacac atttggctac ctctttagtt tgggcatcta tgcttacgcc
    21061 aaccaaaagg gagcaaattt tgaagaagaa tatatccatt tactacgcga tactgcctca
    21121 atgacgacgg aagatcttgc gaagaaacat ttgaatgtcg atcttaccca accagatttt
    21181 tggcaagcag gtatcgacat ggtgactgaa gatatccaaa cgttcatgga attgacagaa
    21241 gaatttatct gatttctatt ttatcacaat aaaaaaagag ttagtccaga atgggctaac
    21301 tctttttgat taatcattct tacatgctga cagcaaagtc aggggcaaac cattgaactg
    21361 aaccaacttt tacgctttcg cctggttgag gagcatggat atattgtcct ccgcctaaag
    21421 caatcgctac gtggtaagtt ccgccttgtg acccccagaa taacaagtct ccagcttttg
    21481 cttgagaaac tgaaattttt gttccagctg attcttgagg aactgtccaa ccaccgatgt
    21541 cacgaccagt tacttgcatg taaacatagc gtgtgaaccc tgagcagtca aagccacttg
    21601 gatctttacc gccccaaaca tatggagtac caatatattt gtaagcttct gctacgattg
    21661 ctgcaccatt cacacttcca gaaggagctg gagcaggtgt tacaggtttt ggttgttcag
    21721 gtgttggtgt tggcgttgga gccggagtac tacttcctgt gccgtttcct gtatcaacac
    21781 tttgatctgt tgatggtgtt gtaggcgcag gtgttgttga ttcttctgtt gttgaagatt
    21841 caggc
//
LOCUS       DACDYJ010000045        20465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDYJ010000045 DACDYJ010000000
VERSION     DACDYJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.045"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            116..1081
                     /locus_tag="IWM51_001774"
     CDS             116..1081
                     /locus_tag="IWM51_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9971196.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(1138..1368)
                     /locus_tag="IWM51_001775"
     CDS             complement(1138..1368)
                     /locus_tag="IWM51_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP9971197.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1454..2341)
                     /locus_tag="IWM51_001776"
     CDS             complement(1454..2341)
                     /locus_tag="IWM51_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP9971198.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(2430..3653)
                     /locus_tag="IWM51_001777"
     CDS             complement(2430..3653)
                     /locus_tag="IWM51_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9971199.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3670..4749)
                     /locus_tag="IWM51_001778"
     CDS             complement(3670..4749)
                     /locus_tag="IWM51_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9971200.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(4880..6607)
                     /gene="ptsP"
                     /locus_tag="IWM51_001779"
     CDS             complement(4880..6607)
                     /gene="ptsP"
                     /locus_tag="IWM51_001779"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP9971201.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(6607..6873)
                     /locus_tag="IWM51_001780"
     CDS             complement(6607..6873)
                     /locus_tag="IWM51_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP9971202.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            7352..9586
                     /locus_tag="IWM51_001781"
     CDS             7352..9586
                     /locus_tag="IWM51_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9971203.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9734..10054
                     /locus_tag="IWM51_001782"
     CDS             9734..10054
                     /locus_tag="IWM51_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9971204.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(10259..10483)
                     /locus_tag="IWM51_001783"
     CDS             complement(10259..10483)
                     /locus_tag="IWM51_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP9971205.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(10681..12261)
                     /locus_tag="IWM51_001784"
     CDS             complement(10681..12261)
                     /locus_tag="IWM51_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP9971206.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12662..14038)
                     /locus_tag="IWM51_001785"
     CDS             complement(12662..14038)
                     /locus_tag="IWM51_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9971207.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(14166..15284)
                     /locus_tag="IWM51_001786"
     CDS             complement(14166..15284)
                     /locus_tag="IWM51_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9971208.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            15426..15860
                     /locus_tag="IWM51_001787"
     CDS             15426..15860
                     /locus_tag="IWM51_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9971209.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(15938..16471)
                     /locus_tag="IWM51_001788"
     CDS             complement(15938..16471)
                     /locus_tag="IWM51_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP9971210.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16565..17743)
                     /gene="mutY"
                     /locus_tag="IWM51_001789"
     CDS             complement(16565..17743)
                     /gene="mutY"
                     /locus_tag="IWM51_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP9971211.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17736..18533)
                     /gene="recX"
                     /locus_tag="IWM51_001790"
     CDS             complement(17736..18533)
                     /gene="recX"
                     /locus_tag="IWM51_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP9971212.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18640..20022
                     /gene="rlmD"
                     /locus_tag="IWM51_001791"
     CDS             18640..20022
                     /gene="rlmD"
                     /locus_tag="IWM51_001791"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9971213.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(20196..>20465)
                     /locus_tag="IWM51_001792"
     CDS             complement(20196..>20465)
                     /locus_tag="IWM51_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP9971214.1"
                     /translation="ITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKA
                     TVPPKTVNQTITLPQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT         6088 a         4155 c         3570 g         6652 t
ORIGIN      
        1 catattttga ttatcaaagt attttctgtg aacagaacta tagaattccc taaaacttgt
       61 ccattatttc aaaatttgtt atagtctttt tattcaaagc gtaaaaggag atcctatgtt
      121 aaaagaaaaa atgaaaactc tccccatgct gccgggtgtc tatcttatga aagaccgaga
      181 tggtaaggtc atctatgttg gtaaagcaaa acggctcaaa aatagagtaa gcagctattt
      241 tcatcagaat aaacagcatt caaaaaaagt attacgtatg attcatcata taacagattt
      301 tgatttcgtc gtagtagaca cggaattaga tgctttattg ctcgagtgtc aattgatcca
      361 acattaccgt cctttttata accgacaaat gaattatttc agtaattata attatgtaca
      421 tattaccaat aagggatttg tattaacaga tactcctacg gctcgaacat acgggccttt
      481 tcgtctctat aaaaaaatgc cgtctatttt gcgtataatg gaagaaactt atcaaatgcc
      541 ttggttgtct gaaattagtt tgttagcttt gcgagtccag cttcctgacc tacaggaaat
      601 gacttttgaa cagaaaaaaa aggaactcca agggctgttt caaggccgaa ataaaaagct
      661 actgacttac ttgaaaaaaa gacaacaaca tttcatctat cagttgaatt tcgaaaaagc
      721 cggtatgctt caaaaagata tcgaattagt tacttatttt attagacgga tccaggaaca
      781 aaagcagttt ttaagaacac catcgctcac cttcagcatg ccattagcag cagatgagag
      841 tcagaaaaag cactacctcg tttgttatgg tcaacttgca gaaacaatga ttacctctgg
      901 tgacgtttct cctgacttct attatgaaaa gaaagaagct catttatctt tgaaacgtca
      961 attgtcaaaa gaagaaatcg atcctgtaca aatcttgatc agttaccgca aaaaattaga
     1021 aaaagaacag tttgaaattg aactgttaaa caaaaaagaa gctgaaaagc aactaaatta
     1081 gttgcttctc ggcttctttt aggcatcttc aacttcaaat gtcaattatc ctaaaattta
     1141 ttggattaag tcatagattt ccatcgctac gatatcgata ttatcaaatt cgtattgctg
     1201 atgagtatct gtttcagtta attcaaatgt atcggtcgct ttatcataag ttactgtaca
     1261 cttctctacg ccttcttttt caaaacggcg aacttgcagt tcattatctg ctgactcgac
     1321 catagcctcc agtcgtttta tgattgcgac taattgtgaa tgtgtcatcg gaggtcctcc
     1381 ttttcttatt tctgttatat tgtatcaaaa gcatagccta cttgcattga ttttctctaa
     1441 aaaaactcgt tttttatgat tttttttctg gtatttttcc ttcaggccac caaagtttga
     1501 tcaaagcttg ggttccgtca ttctcaaaac ctttatctgc caactcttca tatagtttca
     1561 atgcttgggt agttgctggc aatacaagat ccattttttc tgcttcttct aatgcaatct
     1621 ttaaatcttt gataaaatgt ttgacaaaaa aaccaggtgt gtaatcttct tttaaaatac
     1681 gaggagaata attgcttaaa gaccagttag ctgcacttcc acctgaaagc gtctcaatca
     1741 ctttctttaa atcaagtccg tttttttgtg catagacaag catttccgtc attcctgtca
     1801 tcgtacctgc aatcatgatc tgattagcca tttttgtatg ctgacctttc ccagcagaac
     1861 catgcaatgt aaatgtcgta ccaaattctt tgaataaagg cagtacttga tcataaacgg
     1921 cttcttctcc accgaccatg atcgtcagtg taccattctt tgctcctaag tcaccaccgg
     1981 ataccggtgc atctaacgca tctgcgcctt tttcttttgc tgtttttgca attttttctg
     2041 ccaggcttgg tgtacttgta gtcaaatcaa ctaaaatatg tcctgagata tctgcagaaa
     2101 aaatccccga ctgtccatag tagatttctt ctacatcttt ggggtaacct accattgtaa
     2161 agagaatatt actttgttct gctaccgctt ttggtgtatc tgcccagata gctccttctg
     2221 ttactaaagg atcagctttt gcttttgtcc gattatagac agctacttcg tgtcctgctt
     2281 ctagtagatg gcgtgcgaca gcacttccca tgacgcctgt tccgataaaa ccaagtttca
     2341 ttttttcgac ctcctaaaaa tcaaaaatcc ttgcttctat gctagtatac gctagaaaca
     2401 aggatttttt cataaaaaat gtttgaattt tatcttttaa atgaatttaa ttttattttg
     2461 attcgttcaa tcgagtcttc ttcttctttt cttcgcatct cttcttcatg ataaacaacc
     2521 atgtcttgat aaaattcgat catagcttcg ccgaaatgtg tagatgaaat atcataaagt
     2581 ttagcttcta aaacttttgg atctttagga acttcactgg aaatataatc aataagtgct
     2641 ggtgcaaagt cttcgtcggt ttcaaaagtt ttccctaatg aaggatgatc gaacaatcgg
     2701 ttcaaataag catttccttc cacgacacaa ggtgttccgg ctgccatcgc ttctgtataa
     2761 gtaagccctt gtgtttctga agtagaagca ctgacaaaat aatctgccgc tttataataa
     2821 agtgctacat cattgttgtc cacttctcct gtgaactgca caaattcttc taactgcagt
     2881 tctgagatca attcctctaa ctcttctaca taaggtcctt ttcctacgat aactaatctg
     2941 gcgttaggat aagcggagat gacctgagga aacccattaa tcaaggcttg gatatttttc
     3001 tcataagaaa tcctgcttaa agaaagaatc atcaaatggt tttcctgaag acctagtgac
     3061 tcccttaatt tgctgatatc ctgctgagtg atatcagaac gtttgaattt ttcaatgtcg
     3121 ataccagtag gaatcacgcg aagcggtgct attacaccat agctgcgtaa tgtatcaata
     3181 acacgttcac ttggacaaac tacacctgtc gtatgatttg caaataatct agacaagtat
     3241 tttacatggg attgcctgat cacttttcct ttaccgatat aatgaaggta gtcttcatac
     3301 atcgtatggt aagtatggat cagaggtatt ttcaacttct tagctaccat cttcccaagt
     3361 atccctgctc cgaattctgt atgggtatga ataagatcta gttctagttc tttggcaacc
     3421 aaataagcat accacatgcc tctaacaaca actcttcgat ccttgaaaga aataaatggt
     3481 acacttggca ttcgaatcac atctttttcc aacttatccg cattaggatc ggtcgttgta
     3541 aaaatataga cttcatgccc atgtctttct aattcttgtt ttaatgtttt gatcgatgta
     3601 gcaactccgc tgacttgcgg aaagtaggta tcggtaaaaa agccgatttt catgtttttc
     3661 gcctccaaac tagtccttga cctgatttac tttttttaat ttgctagaga ctttttcatt
     3721 cagtacactt tcgtatacat ttttaagttc ttcaccgatt ttattgatac ttcgctcttt
     3781 tgctgtttga taaccggctt gtgtcgtact cggtagttct tgattggtaa tctgttggat
     3841 cagtcggata aattcttcgt ttgtcttccc catatagcag ttcttgctat cttctaacca
     3901 gccattatac actggaatat ctcgaacaag tacgttttgt ttgcttgcta aagcttctaa
     3961 aacaacgatc ccttctgtct cttcataaga agggaaaaag aatagatctg cacctgaata
     4021 cgcaccttct atgatctctc cacgaatgta accaggaaac aggacatttt caggatgatc
     4081 ttcttttaca attttccgga tgttccgagg aatcgagtac atgggaacat gaccaaacca
     4141 aataaatgtg tattcgggca tcttcttagc aatttctacg aaatcaatga ttcctttacg
     4201 ttcgaaaaac aaaccgacac aaatgattac tttttgatcc ggagacagtt tgaaatattc
     4261 tctgaacttt tgttctttca ttggatcagg gtgatatttc tctaagtcga tcccattgga
     4321 aatagcttga atcggcacac ggataccata gctttccaac aactgttttg aataaggtgt
     4381 tggcgtgatc agatagtccg ctttttggta caatcctacc agatattttt ttacgatagg
     4441 agaaagctgg ttcgatccga taaaggaatt tcggaaatct tcctctgtag aatgcgcatg
     4501 atagacaact ttttttcctt ctcttctggc tttattgacc atattatgac tattgattcc
     4561 gtatgtattg atgtgcagga tgtcataatc ttctttttca tctagtgtat aagagatacc
     4621 gacttccgtc agagcacgtt tttgatgatc aagcgcacgc ccgattccag attttgcaag
     4681 catcttttca ctttcaaaat ataataatac cttcacctta cgtccccctt cacttatata
     4741 gtataacata gcctacaaac tgtcactcaa acttaagccc tatttttgtc tattcttact
     4801 gacaaaaacg ttttcttaaa aaagaacaaa aaaactggcg tagctctttt acaagccaca
     4861 ccagcttttg atacgttgtt tattttacat attcatgaac aagttcaacg acttcttcca
     4921 tcgtatcgca ttctttcaat gcacgatcag caagttcagc cattttcgct gtgtccaagc
     4981 gtttcattaa actacgcgtt ttaagaatag atgtcgcact cattgagaac tcatctagac
     5041 ccatacctac tagcaatgga acagctgttt gatctccagc catttctcca cacatacctg
     5101 cccattttcc ttcagcgtgt gctgcatcaa ttacattttt gatcaaacgt aaaattgatg
     5161 ggttatatgg ttggtacaag taagaaacgc gttcgttcat acggtctgct gccattgtgt
     5221 attgaatcaa atcgttcgta cctacagaga agaaatctac ttcttttgca aacttgtctg
     5281 ctaagactgc tgctgctgga atctcgatca tgatgccgac ttggatcgta tcagatactt
     5341 ccttgccttc acttatcaat ttttgttttt cttcttcaaa gatcgctttt gctgcgcgga
     5401 attctttgag tgtagctacc atagggaaca tgatacgtaa attaccatga acagatgcac
     5461 gaagcaatgc acgcatttgt gtacggaaca tttcgtcgcc ttgttcagat aaactgatac
     5521 gcaatgcacg atatcctaag aatggattca tttcatgagg taattgcaga tatggtagtt
     5581 ctttatctcc accgatgtcc attgtacgaa caactacagg ttttccttcc ataccttcta
     5641 atacagcttt gtaagcttca tattggtcat cttctgttgg gaagtctgga gaatccatat
     5701 acaggaattc agtacggtac agaccaactg cttctccacc attattatgg acaccaacta
     5761 gatcttttgg tgttccgatg tttgcagcta attcaaaatg cttgccgtca gctgtgacag
     5821 tttctgcatt ttttaatttt tcccattctg ctttttgtgc tgcgtaatct gcacctgctt
     5881 tttcaaattc agctttttgt tcatctgtag gatcgatgat tacatcgcct tcgatcccgt
     5941 tgacagccaa aatatcgcct tctttgactt tagcagtaat ttcttttgtc ccaacgattg
     6001 ctggaatttc taaagaacgt gccatgatag cagaatgtga agtacgtcca ccaatgtctg
     6061 ttacaaacgc ttttacaaaa ttgcgatcta attgtgctgt atcacttgga gtcaagtcat
     6121 gtgcaacaac gaccacttct tcgttgatca ttgatggatt tggtaaagta acgcctaata
     6181 agtgcgctaa aatacgttta gcaacgtcac gaatatctgc agcacgctct tgcatataag
     6241 cattatcttc cattgcttcg aacataccga tatacatatc cgttacttct tttagcgctg
     6301 attcagcatt tacgctgttg tctttaatat tttgcttgat ttgacctacc atttctggat
     6361 ctgaaagaac catcaaatgg gcatcaaata cttgcgcttc tgcttcacct aagctttgcg
     6421 ctgctttttc acgaatttgc tgcaattctt cagtagattt agctaatgca gcatccaaac
     6481 gagcttcttc agccgcagta tcttctactg ttgccttact gaatgttaaa tccggttgaa
     6541 ctagcaggta agcttttgca acggctacac cgtcactagc ggcaatccct tttagcattt
     6601 caaccattat tcagataagc cttctttctt cattgtttca acgattgctg ccattgcatc
     6661 agcttcgtca gcaccttctg cagtaatagt aacatcagaa ccttgaccta cacctaaaga
     6721 cataacgccc atgattgatt tcaagtttac tgatttacct ttatactcta agtttacatc
     6781 agagttaaat ttgcttgctg tttgtactaa tagtgtagct ggacgagcgt ggatcccagt
     6841 ttctgctact acgtgaaatt ctttcttttc catattaatc ggtctccttt aagtgagtgt
     6901 tgtttttatg tgagggtttt cttttttagt aattcagaga atcctaacaa atgccaccct
     6961 ttcatctgta aagattaaca ttttttttcg ttagatacaa cagttttcat tcaatttcag
     7021 ataaaagtat tcaaattatt ccggattctt tttcatcctt ttcttcacat tttataacaa
     7081 acgtgtttat ttcctttttc ttgtcgcttt ctcttctcct gacgtttatc attctttcct
     7141 ttgaccctct ttgttttcat atgaaacgaa ttacttgctt ccctttttat ttttagtata
     7201 ataagagtca aaggtcagga atggtcaata gagatttcac cttttctacc tcataaatct
     7261 cttaaaaaac taggggttat tcttttacct catgaaaaac gagatgtaaa atatggatgt
     7321 acccattttt gtgaaaggac gtgaatgatt catgctttgt caaaactgcg gcaagaatga
     7381 ggcaacgatt cacttgtatg ccactgtcaa tggtcagcgg acacaactcg attactgtca
     7441 gagctgttat caaaaaatca aaaatcaaca aaatggaggt ctgactaata tggcccaaaa
     7501 tgatccattt ggatttggca gtttggacga tcttttccgt tccatgtctc gccaaatgca
     7561 acagcaagga aattatgagc aaacaccacc aacacaattt ggcggaaaca acaacttcaa
     7621 tggtggtcag ccacctcaag gcaatacaga aggtttattg ggagaatacg gaatcaatat
     7681 cacagaacaa gcacgacaag gtgatatcga tcctgtgatc ggacgcgatg acgaaatcaa
     7741 acgagtcatt gaaattttaa atcgccgaac caagaacaat cctgtcttga ttggagaacc
     7801 aggtgttggt aaaacagcag ttgttgaagg tttagcacaa aaaatcgtag atggtgatgt
     7861 accgcaaaaa ttaatggaca aagaagtcat tcgtttagat gtcgtatctt tagtccaagg
     7921 tactggtata cgaggacaat ttgaagaacg tatgcaaaaa ttgattgaag agatccgtca
     7981 agcagaaaat gtgatcctat tcatagatga agttcacgaa atcgtcggtg ctggttctgc
     8041 tggtgatgga aatatggatg ctggtaatat tttaaaacca gctttagctc gaggagaatt
     8101 gcaaatggtt ggtgctacta ccttaaatga gtaccgcatc atcgaaaaag acgctgcttt
     8161 agaacgtcgt atgcaacctg tacgggtaga tgaaccaact gtcgaagaaa cgatcagtat
     8221 tttgaaaggt ttgcaaaaac gttatgaaga ctatcatcat gtaaaatata ctgatgaggc
     8281 catcgaagca gccgcaactt tatccaatcg ttacgtccaa gatcgcttct tgccggataa
     8341 agccatcgat ttgctagatg aaacaggttc taagaaaaac ttgacgatcc aaatcgttga
     8401 tcctaaaaca atcgagaaaa aactgcaaga agcagaagaa caaaaagttt tagcttctcg
     8461 agaagaagat ttcgaaaaag cagcttatta tcgcgatcag atcaataaac ttcaaaaaat
     8521 gaaagaacgt cagctcactg aagaggaaac acctgtgatt accgaaaaag atatggaaaa
     8581 aatcgttgaa caacggacag gtattccagt gggtgaatta aaagaaaaag aacagacaca
     8641 actgaaaaac ttagcagatg acttgaaagc tcatgtcatc ggacaagata acgctgtaga
     8701 tcgagtagcg aaagcgattc gccgaaaccg tgttggatta aacaaacaga atcgtccaat
     8761 cggctccttc ttatttgttg gaccaactgg tgtaggtaaa acagagcttg cgaaacaact
     8821 tgcctacgaa ctattcggtt cacaagattc aatgattcgt ttcgatatgt ctgaatatat
     8881 ggaaaaacat agtgtctcta aattgattgg ttctcctcca ggttatgtcg gatacgaaga
     8941 agctggtcaa ttaacagaaa aagtacggag aaatccttac agtcttgtct tgttagatga
     9001 agtagaaaaa gctcatccag atgttttgca tatgttcttg caaatcttag atgatggtcg
     9061 attgacagat gcacaaggaa gaacggtcag ctttaaagat acgatcatca ttatgacaag
     9121 taatgccgga actggtaaag tagaagcaaa cgttggtttt ggtgcggcac gcgaaggtgt
     9181 tactcgttct gtattgaacc aattaaacaa ctactttaca ccagaatttc tgaaccgttt
     9241 tgacgggatc atcgaattca gtgcattatc aaaagaaaac ttgatgacaa tcgtcacact
     9301 aatgctagac gatgtcaacc aaatgctggc agcacagcaa cttcacatcg aagttcctac
     9361 gaatgtcaaa gaaaaattag ttgacttagg ctatgatcca tctatgggtg ctcgtcctct
     9421 tcgacgcacg atccaagaac aaatcgaaga tggtatcgct gaattttatc ttgatcaccc
     9481 ttctatccat gagttgaaag caaaactgga taaagatgga aaaatcatcg ttacttctaa
     9541 accagaacgt ttggctaaag aatcagccga agaaacggct gaataaatcc agttttatag
     9601 cgtgagacgg tatttctttg agaaatgccg tctcattctt ttttcagaaa agtcaaaata
     9661 ctgttcattt tataaaaaag aaattataat aagagaaaca agcaattaaa aatacataaa
     9721 agaggtgagc gccatgaaat tggtaaatgt aacaaacagc cattcccgtc ttgtattaaa
     9781 ccagcttgaa aacactgatg cccatctagt caaggtctac acagccggaa ataccactat
     9841 tgtctacact gaagcccctg aacataatga aattctgctg atcaatgaca aacgtaaaat
     9901 ccagccgaaa gaaatcgaag atgctaaaaa ttatttctta gataaaatca aagacgccgt
     9961 ttaccacgag gatgaaatca aagtaatcga attggcaggc cttgttgaaa tctcgattcc
    10021 aaaacatgaa ttaggcgcta cattagccgt gtaaacaaat tttgaacata ataattaaag
    10081 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcga
    10141 aagcagaagc aactccttcg gaaataagct gaaattcacg aaaattgctt ttcaaatttt
    10201 tgtgaattcc agcttatttc ttggagttaa acgcttctgt ctcagcctct ttttctttct
    10261 agctttttga tttttctctt ttcttttgta cttttctctt ttcattttca gttctacgcg
    10321 tgtctttatt tttaactttt tcctctttat ggatgattga tcgtactttt tgtgcaattt
    10381 gctcgttttt gtcccagtct tttacatcta ataatcgaca taatcgtaac atctcttctt
    10441 ttttctgttt ttttctttca cgtctttgcc attctgtttc catgtgtcac actctccttt
    10501 ctttaatcat atgtacgcaa aataattttt atattttttt gattagacat ttctatgatt
    10561 ttaatatagt aaaaagagcc gtcacggaaa atccgtcacg gctctaaaca aaatatgaga
    10621 ggctgttttt gtttggctat tcttattact tgatgcaggg cagcttgtgt caagacttct
    10681 ttacatcaaa ctttttaatt ttacattggg atatttatcc gcaaaccagc gttctgcaaa
    10741 ttggttctcg aataagaaca acggttggtc gaaacggtca cgagccaaga tattgcgact
    10801 tgaactcatc ttctcatcca aatcttctgg atcgatccag cgagcgattt tgtgtcccat
    10861 tggagacatg attacttcgg cgttatactc atttaacata cgatattgga atacttcgaa
    10921 ttgtagctga ccaacagctc caatgatata ttcttccgtt agatatgttt tataaagctg
    10981 aatcgctcct tcttgaacta attgatagat tcctttgtgg aaggattttt gtttcatgac
    11041 attttttgca gtcactttca taaacagctc tggagtaaat gaagggagtt cttcataagc
    11101 aacttttagt tttccttcga acaatgtatc cccaatctga taattgcctg tatcatatac
    11161 accgatgata tcgcctgcta ctgctttttc gacgttttct ctcgaatctg ccataaattg
    11221 tgtgacattg cttaatttca gttttttatt tgtacgttcc aaccagacat ccattcctcg
    11281 ttcgaatgtt ccagaacaga ttcggacaaa agcgattcgg tcacgatggg ctggattcat
    11341 atttgcttgg atcttgaata cgaaacctga aaattcttcc tcataaggac ttaccatctg
    11401 atcatcttgt gttttgtgtc cataaggagc tggtgccaat tcaacgaaag tctctaaaaa
    11461 agtctgtaca ccgaagttag tcaatgcaga accaaaaaat actggtgtct gatcgccacg
    11521 agcaattttt tcttgattaa atgcatctcc agcctctttg attagttcta cttcttctaa
    11581 gacttgttca tataaggaat tttcagttag aggatgattt tcgggaagca gtccatcttc
    11641 atccaattct actaatcgtt catcctgata attttccgga cgataaaact ctacacgatt
    11701 atggtagata tcataaagac cttctagccc tttacccata ccgattggcc aattcatcgg
    11761 ataagattcg atatctaata gctcttctaa ttcttctaac aattccaatg gttcacgacc
    11821 atcacgatct agtttgttga taaaagtaaa tatcgggatg ccacgttttt ttacaacttg
    11881 gaataatttc tttgtctgtg cttcgatccc ttttgcacta tcgattacca tgaccgcact
    11941 atccacggcc atcaaagtac gataagtatc ttcagaaaaa tcttcatgtc ctggtgtatc
    12001 caagatattt actcgtttcc cttgataatc aaactgcata acagaactag taacagaaat
    12061 cccacgttgt ttctcgattt ccatccagtc tgatttggca aaattacctg tttttttgcc
    12121 ttttacggtc ccggcttgac ggatagcccc accaaacaat agtaattgtt ctgtaatcgt
    12181 tgttttccca gcatccggat gggaaatgat cgcaaaagtt ctgcgattgt ccacttgttg
    12241 ctttaatttt ggattattca taacctaact ccatttcttc cattcaatta tttatatttt
    12301 ctctaggcac agattgtccg atctcttaaa gaaagacaat ttcatatagg gctgtgacaa
    12361 aagccgtgta gcagtctctt tttccgagta aacggcgttc tagagacagc ctatcagcaa
    12421 taagcccacc caagccaaaa atacgaaaag ctgtttttgc ttggctgctc ttattactta
    12481 atgcaggata gctttttcac aacctttgtc aactactaca actgaatcag tatagcatgg
    12541 attcagcgaa tgaaaaagcc accaaaccat aagagtcaaa tatttttttg tcagctgact
    12601 aaaagaaaag acgtgttgct tgtttattca agcaacacgt ctttcataac caagtttcca
    12661 attatcttct tggctcgtca tcttcgaatt cttttctgaa gtatcttcgt tcttcttcag
    12721 gttccgcttc tgtttcttcc ggatcatgaa aaatgacacg gattttaagg attcgagaac
    12781 cttccatttc ttcagaaacc agcgtaatat tatcgacatc aaacgataac ttttcgcctt
    12841 catccggaat catgcctaat gctgtaatca aataaccagc catcgtatct acatcactca
    12901 tatgcagatc actgtcaaaa gcttcattga actcatcaat cagcatacgt ccttggatga
    12961 tatattcgtg ttcatcgatt ttttcataaa gattctcaac ttcatctgat tcatcatcga
    13021 tttctccgac gatttcttct aaaaggtctt ctagtgttgc taacccaacg acgccgccat
    13081 attcatctaa taaaatagcc atctgattct gcgtcttttt catttcatat aacagatcat
    13141 caataaagac cgtttcagga acgaataaag gctcctgcat gattttcttg atatctaaat
    13201 tatcaaagcc gaatttatgg gcagctttta gtaaattctt tgtatgaagg attccgacta
    13261 ctttatcttt gtcttcgtta taaacaggga ttcttgaata gttttcggat aaaacttcat
    13321 tcacattttc ttccacagtg tcgttgatat caatcataaa tgcatccgtt cgtgggacca
    13381 tgacttctcg ggcaactttc gtatcaagag aaaaaacccc ttggagcatt tctaattctt
    13441 cattattcaa tacgccttcg ttttcaagca tataacgcat ttcatctctg gtcatttttg
    13501 aatcttcatc atcaaaagtc atcggcgtga tacgcgccaa taaacttgtg gaagcagaaa
    13561 gcagccagac gaagggctta gctactacac caatcaagcg tatcagccct gaagtgaact
    13621 ttgcgacttc ttctgattta tttaaagcaa tacgtttagg gtacaattct ccaaaaacaa
    13681 tcgatacata ggtcaaaata gccagcacaa tgatatttgc aatatttttt gccgctgtgc
    13741 cgccacctaa taccggagct aaccgttccg ataaagtatt agctagtgaa gcaccagaca
    13801 aaatgttgac caaagtgatt cctacttgga ttgtcgatag gaaattattt ggattctgca
    13861 atatttttaa aagcttttga gatttcacat ctccttcatc agctttttgc tcgattcgat
    13921 tcttattgat cgaaacaaca gcaatttcgg aagctgctaa aaaagcattg atcaatgtca
    13981 aaacgatcaa taataaaatc tgcgctaaca gcgactgact ctcagggtca gcattcataa
    14041 tcgcattctc tccttataaa attaattttt agtttacgat agaaaaaaac gtggcactat
    14101 agccacgtta aaatggtatc acaaagcaat tttctttgca ataaaaacaa gtgtactaaa
    14161 actacctacg aggtcgtagc aattttcttc ttgttttcat tttgcttgtc ttctttgacg
    14221 attttgatta tataggaaat gatcgtcttg cagattgcgt aaaaaggaac acctaagaag
    14281 ataccaagca atccggcaat atttccagct actagaagaa cgataatgat cgttaatgga
    14341 tggattttca gtgatttccc aattacatta ggataaatga tattcccatc caactgttgg
    14401 acgattagca caaccacaca acaaagtaat gcttttaccg gatcattgaa gactgttact
    14461 agaaatgcag gtgctagtcc tagataaggt cctaaatatg gaattaggtt tgtcaatccc
    14521 gcaatgaccc caaataagaa agcataatcc acaccaatta ccatatatcc taaaaatgtg
    14581 aaggtaccaa cgaataaaca ttcgattgct tgtccgctaa tgtaattagc caatgtttta
    14641 ttcagttgcc ccaataactc cacaatttgg tttcttcttt tttctgggaa aaactgttgg
    14701 atgtttggca ctagtttttc tccgtctttc aacatataga acaagatgaa tggtgctgta
    14761 atgattacta cggttgccga agcgatcgtt ccaacgatcg aacctaaact attggatact
    14821 ccactaagga attgctgaat gattgtcccg taagaaatgt ctaattgctc aaaatattta
    14881 ttgatatcta cttccttgaa caaaggcaat tcggctacct cttttaacca gctttctacc
    14941 tgtttgatga aatcaggcat attagatgcc aatgaggaaa tttgctgtac tagacttggg
    15001 atgacactaa gtaaaatcca aataattgct gcaactaaaa ggagcagcac aattgcgaca
    15061 gcccatattc ttttgatttt tgctttcatc aacagcttga caataggatt caataaatag
    15121 taaagaaagc ctgcaatcag tacaggtgca aataacgtgg aaaaaaatgt cccgatcggt
    15181 tcgaaaataa agtttatttc tgatgaaatc atgatcaatg cagctatgat caataattca
    15241 agcgaccaaa acattagttt agactgtcta agtttttcaa acaatagatt tcctcctttc
    15301 tttcttacta gtatatatta tattcgttaa aaattctttt attctttttt tatttttgaa
    15361 aacttgccat actttttatt tgttttattt tccagtatac tatacgaaag gaaggtggct
    15421 agaccatgaa agttgtacaa acaaaagata caatgagcaa tatttactta gatgctgtac
    15481 ggatcaggca tcaagttttt gtagtcgaac agggtgtgcc tttgtctcga gaaatagaca
    15541 aagatgaagc ccattgtatc cacttcgtcc tgtactcaga taaaaaagaa ccgcaaggaa
    15601 ctgtacgtct tttgccttta gaaaacggaa aaatgaaatt acagcggatg gcgattcttt
    15661 ctgaatacag acaccaaggg ttaggaaaaa tattgattga agaagcagaa aactttgcta
    15721 agaatcaagg ctataatacg attttattag gggcacagtc cacagctgaa actttttacg
    15781 aaaaactagg atatacagct tacggcgatc cttttgaaga tgctggcatg cctcatattt
    15841 atatgaaaaa gactttataa aaatttattt cgaaaaaagc aaaaaacagc aagcttttga
    15901 gaaaaatctc tcactggctt actgttcttt tttgtgttta cttacgcgac aattgcttgt
    15961 aacggttata ccaaatatca atatagcctt ccgagaacgg gccatctcca ttattgatcc
    16021 agtcaaccag aatcttcaca ttttctttta ggatgaagtc aatatcgttc gggtaattca
    16081 tcattttgct gtgcatctca tattcatcga catccaatag tcgtttctcg ccgtcaggaa
    16141 ataccttgat atccaagtca tagtcgatat atttcaaggc ctcatcgtcc agcaaaaaag
    16201 gtgaagctag attacaataa taggaaaccc ccttttctct gatcattgct attacattga
    16261 accagtattt tttatgaaaa taaacaatcg ctggttcgcg cgttacccat cggcgtccat
    16321 cagattcagt gactaatgtg tgatcattca cgccgatcat agagtgttcg cttgttttta
    16381 ataccatggt atctcgccac gttcgatgca atttgccgtc gtgcttgtaa ctttggatcg
    16441 tcacaaactc tccttctttt ggaactccca tagcaaccct tccttaagca gcattttgtg
    16501 ctttgtgttt gggtgttcat tacaccgaca tcataattat tatatcaata aaaatgagct
    16561 ttgcctagaa aggattccta ttgtttcgat ttttctctaa ctgatctacc agtttcattt
    16621 ggaccttagg aaaaacgacc gaatcaaatg catcaggctt caaccacttc gaagttttgt
    16681 tctcggtaaa ttcttgttct gctccctcgg ttgcccgtcc gtaaaaaagg agaacatgcc
    16741 atttcagatg actaaaaata tgagtaactt ctcctaaatg tcgtgtctgc caaacaaaga
    16801 gttcctgctt ttcaaatggg agattttgct cttcttctgc tactaggtca tcaaacagat
    16861 cgatttctgg ctttgtttcc cattcttttt tcaatcgctc atactcctct tgtgtcactt
    16921 ccatcattgg aaatgtccac atattcgcca gcaatttttg gctatctctt tcttcaaaat
    16981 aatacgctcc agaatgattt tgaagagctg cactgatata ataaacgtct tttggctttg
    17041 ctttttttgt ttttacagga aaactcgttt ggattcctcg tttgttagct aaacaaaagg
    17101 cttggatcgg acaagtctca cactttggag aagttggtgt acagatagct gaacctagat
    17161 ccatcattgc ttggttgaat tcacctggat gcttttcatc aatgattttc cgcatcgctt
    17221 catcaaagat tttcctgcta gaagcttttg cgatgtctgc ttcaatgcaa aacaatctgc
    17281 tgactactcg catcacgttg ccgtcaactg caggttctgg aagtccaaat gcgatacttg
    17341 caatcgcgcc tgtcgtatat ggtcctattc ccttcaatga gctaatttct tcaggcgttt
    17401 gcggcatttc cccatcaaat tctgacatga tctgtttagc agcagcttgg atgttccggg
    17461 ctcttgagta ataaccaagg ccctcccatg cttttaaaag cttttcttct ggagcatttg
    17521 ctagctcttc aattgtagga aaccattcca tgaaacgata aaaataatcg atcactgtgt
    17581 ctactctggt ttgctgaagc atgatctcag agatccagat acgataagga tcacgattat
    17641 agcgccaagg caggtttctt ttttcttgtt cataccattg aatgaactga tcttgaaatt
    17701 cttttgtttc tttatctgtc catttttccc attctttatt cactttcctc ttccttttcc
    17761 gtaatgaatc gctggatcat atccaaatca aatccttttt gatataagct ttgctttact
    17821 tttagatttc tttttttagg ttcaaacctt aaatttcttc gccatagttt atctccttgt
    17881 ttgactaaat aatcatattc tatttcttga tctgcttctt gcggaagatc agctatcgct
    17941 tgttggatca cttcttgaga aaagcctttc gtcatcaatg tttgttggat ttttctcaat
    18001 aattcttttt ggctttttcc gtgatttttt ccaaacgctt tttcggctgt ctgatgtgca
    18061 ttttgaactt ggtcttcaaa tgtatattgg ctgagcgctt gttcaatgat ttcaggtttc
    18121 aaaccttttt gctgcatctg ctgcgctaat ttctttggtc ctttgtcact taagcgcata
    18181 ttcgtgcgca cataactttc cccatagacc aaatcatcga caaggtccaa ttcttttaag
    18241 cgggctacga ttttatttcg atcttcttga aggatttcct tttcttttaa gtatgttcgg
    18301 atttcttttt ccgatcgcaa ctgataactt aagtaattca ttgctaggtg aaaaccgaaa
    18361 tcagctccgc tactcttctt gatctcttgg atagtttctt cgtctaattc ccgtcctttt
    18421 aataatcgat agcgtactaa tacatcttca gatacttgta aagcttctcc ttcagataaa
    18481 tctacgcgat agaaagggcc tttgccttta ctgattctcg taacggttaa cataaacatc
    18541 ctcctcgaac atatattctc tatcatacca cagaaaatcc ccgtaatcag gtattatatg
    18601 ttagaataaa ccacagaaaa aagaaaaagg gagatactta tggcagaaac aattgtcaaa
    18661 actggacaaa cggttccttt gaaaatcaag cgtttaggca taaatggtga aggaatcggt
    18721 tatttcaaac ggttgatcat atttgttcca tatgcactac caaaagaaga agtactagtg
    18781 aaaatcacga aagcaactcc tcgctatgca gaaggagaat taataaaagt caaaaaaaca
    18841 agcaaagatc gcgtagtggc accatgtcct gtttactatg aatgcggtgg ctgtcaatta
    18901 caacacttag cttatcaagc tcaattggat tttaaaaagg atctgctttt gcaagcattg
    18961 gaaaaattca aaccagcagg ctttcgatct tatcaacttt tgccgacgat cgggatggat
    19021 gagccttggc attatagaaa caaagcacaa tttcagttac gaaaaaataa gcagactcaa
    19081 aaaattgaag ctggtttata cgaagcaaac tctcatcagt tagtccctat aacagactgc
    19141 ttggttcaag aacctcgaac acaaaaagta atgaatactg ttgttcaact attgaataaa
    19201 tttgatgcac ccatctatga tgaacggaca aacagtggaa tcttccgcac aatcatggtt
    19261 agaatcggtg ttaaaactgg ggagttacaa gttgttttta ttactcgcag taaaaaattc
    19321 ccgcaaaaaa acgcaatgat ccgtgagatc aatgaacgac tgcccgaagt tgtttcaatc
    19381 atgcaaaatg tccaggcaca aaaaacgtct ctcgtgatgg gggatgaaac gatccactta
    19441 tggggaaaag aagccattga agaacaaatc aatgaagtga ctttcgatct ttctcctcgt
    19501 gccttctttc aactcaatcc tcagcagacc gaattgcttt atggcgaagg aatcaaagca
    19561 ttagatgtcc agcctaatga aacgatcgtg gatgcttatt gcggagttgg gacgatcggc
    19621 ttaagtgtag caaaacaagc caaagaagta cggggaatgg ataccgttcc tcaagcaatc
    19681 gctgatgcaa aaaaaaatgc ggaaagatta ggatatcaca acactcgata tgaagtcggg
    19741 acagcagagc aagttctgcc taaatggttg aatgaaggtt ttcagcctga tggtatcatc
    19801 gtagatccac cacgtacggg tttggataac gcattgatcc aatcattatt gaaataccct
    19861 ccaaaaaaac ttgtttatat ctcctgtaat gtttctacac ttgccagaga tctggttaat
    19921 ttaagtaaaa agttcaagat tgaatattta cagtccgtcg acatgtttcc tcagactgca
    19981 cgttgtgaag ttgtcgtgaa gatgacgaga ctcgctgact aaatgaaaga ggttgtgaaa
    20041 aagtctgccc tgtatcaagt aataagaaca gccaaacaaa aatagctctt catgttttat
    20101 gaatactgta cttgtgaccg aagagttaac aggaggacac tatttattta aaaaaagggt
    20161 tgtgacatag tttttgtcac agcccttttt gattattatt ggatattcac gtctatcact
    20221 tgataaaatg catttgtcgt atcgctgata ttccatactg ctaaaatcac attatatcct
    20281 tttcgatcct gcggaagtgt gatcgtttgg ttgactgttt ttggtggaac agttgctttg
    20341 tcgtctattt ttgtgatcaa ttcaaaatca ctaaatttca aaggttgatt aggattccaa
    20401 gttggtttgg taatgtagta atcccaagta cttgtccgat gttgtgctgt taaattccaa
    20461 gtaat
//
LOCUS       DACDYJ010000046        19476 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDYJ010000046 DACDYJ010000000
VERSION     DACDYJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.046"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            129..3656
                     /locus_tag="IWM51_001793"
     CDS             129..3656
                     /locus_tag="IWM51_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9971215.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3653..7375
                     /gene="addA"
                     /locus_tag="IWM51_001794"
     CDS             3653..7375
                     /gene="addA"
                     /locus_tag="IWM51_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP9971216.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7444..7785
                     /locus_tag="IWM51_001795"
     CDS             7444..7785
                     /locus_tag="IWM51_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9971217.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8094..9137
                     /gene="pheS"
                     /locus_tag="IWM51_001796"
     CDS             8094..9137
                     /gene="pheS"
                     /locus_tag="IWM51_001796"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP9971218.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISEEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9142..11562
                     /locus_tag="IWM51_001797"
     CDS             9142..11562
                     /locus_tag="IWM51_001797"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP9971219.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11732..12742)
                     /gene="trpS"
                     /locus_tag="IWM51_001798"
     CDS             complement(11732..12742)
                     /gene="trpS"
                     /locus_tag="IWM51_001798"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP9971220.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12996..13733
                     /locus_tag="IWM51_001799"
     CDS             12996..13733
                     /locus_tag="IWM51_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9971221.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13747..14562
                     /locus_tag="IWM51_001800"
     CDS             13747..14562
                     /locus_tag="IWM51_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9971222.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14575..15222
                     /locus_tag="IWM51_001801"
     CDS             14575..15222
                     /locus_tag="IWM51_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9971223.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15238..15894
                     /locus_tag="IWM51_001802"
     CDS             15238..15894
                     /locus_tag="IWM51_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9971224.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16056..16877
                     /gene="racE"
                     /locus_tag="IWM51_001803"
     CDS             16056..16877
                     /gene="racE"
                     /locus_tag="IWM51_001803"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP9971225.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16880..18235
                     /gene="rph"
                     /locus_tag="IWM51_001804"
     CDS             16880..18235
                     /gene="rph"
                     /locus_tag="IWM51_001804"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP9971226.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18236..18754
                     /locus_tag="IWM51_001805"
     CDS             18236..18754
                     /locus_tag="IWM51_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9971227.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18861..19361
                     /locus_tag="IWM51_001806"
     CDS             18861..19361
                     /locus_tag="IWM51_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9971228.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6553 a         3343 c         4182 g         5398 t
ORIGIN      
        1 tgcgtatact taaaaaagta gaaatatcag aaccttaaaa ggatataatt tgaagaatat
       61 agaacaaatg ttcagtattt tttgatataa taaaggagca ccgtatcatg tgttgaagga
      121 ggaaaagcat gtctttacaa tttattcttg gaaacgggaa aaaagatcac cgtcaagtct
      181 tgatcgacga agcatccgct tggctcgacc aggatgcaag taaccaagta tttttccttg
      241 ttcccaacta cagcaaattc gaacaggaac aagaaatttt agcggagatg cgaagaagaa
      301 gcggaaaaaa aactttcagc acaacaaacg ttcaagtatt tagtttttat cgtctggctt
      361 ggtatttgct gcaacagaca actcttctta caggaaatga attatcagaa tcaggatctg
      421 caatgatctt gcgacagatt ttagaaaaaa atgaggaaga attaacaatc tttcgcggag
      481 aaatcaataa aagagggttt attcgtcaat tacaagaact ctacagtgaa ttgcgtacag
      541 gaaatattca accagaagac ctgatgcttt tatttggtga ttcacctaat gcagaagacc
      601 agcaacttaa aatgaaagac ttgaagctga ttttttcagc ttatgacagc gaattgactc
      661 aacgtgcact aacaaatgaa gatgcactat ctatactagc aaattatatg cagacacaag
      721 atttcaacaa cgtcaaattt attgtcagtg gtttttctcg aatcaatggt caagagtatc
      781 aattactgca ggcaatgatg gaaaaggggc aattggttgt cgatttattg ctggatcgtc
      841 cttatacagc ggatcttcca gaagtactta cgctctttca tgagactgga aagctttatt
      901 tccgtctttt tcaaagtgca agagaacgga aagtccctat attagtggac cgatttgcac
      961 cagagcagac tcgtccgaca gagcttcaac tgctgcaaac aatgtgggaa gagacatcta
     1021 gccaaaagaa agcggcagta cacaaaatag ggggacaaga gcatctccat ttgtgggaag
     1081 ctgaaaatcc aactgaagaa gtccgacaat tagctgttga aatcagacga ctagtcagtg
     1141 aagaaaacta ccgttatcgt gatatccaag tgctgacaaa aaatatgtct ttatatggaa
     1201 atatccttcg gccgatcttt caagaaatgg caattccttt ttatatcgat gaagaacaaa
     1261 tgatggaaaa tcatccgctg atcgaatgga tcaacagtct gttcgctctt gaccgctatt
     1321 cttatcggct aagtgatatt atgcgttttt tcaaaactga attatttctg tcgacggaag
     1381 cagaaaccga acttgatgaa tggaaacaag agcgagatga attcaggaaa aaactggaca
     1441 tcactgaaaa tgtagcactt gcttacaatt atcaaggaac ttattggact cgtgaaaaag
     1501 actggcagtt tgtggcgtat gattttgaag cagaacagat tgaagacgta cagtatttgg
     1561 aagaacagtc aaatacgatt agacgattat ttcagaaagc tgtaccagta tttttgaaaa
     1621 aaataaagaa aagcaaaaca ggacaagagg ccgcaaatgt tttttatcaa tttttgatcc
     1681 aaagtggcgt acagcaacaa ttattgtttt ggcgtaatca ggaagtagac agaggaaact
     1741 tagaagcggc aagaaaccat gagcagacat ggggagcgct gattgatctg ttggatgaat
     1801 atgtagtaat ttatggtgaa tcggcttttg actggtcact gttccaagaa atcattacaa
     1861 gtggtctaga aaatctaagt tatggtaaaa tccctaccgc aatcgaccaa gtgcgaatca
     1921 atcgtttgga attagttcga ccaaatcaag ctgccgtcac ttttgcatta gggttgaatg
     1981 atcaagtgtt tccagaccga aaagagacaa aaggattgtt gtccagtgaa gaacgagaag
     2041 tattaaatca ggccttgcca gatggacagt ttttattcga cccatcaaag gaaagtatct
     2101 cttttgagcc tttccaagcc tatcttgttt ttctttccgg aacagatcga ttatatctta
     2161 gttatgcaca aagctatgac acagatggtt cgctgaagat gtctccttat ttgcgtagaa
     2221 tcacagaata tttatctgtt cccgtcgaga gaaaagagca tttgacgatc gaaagcgacc
     2281 ctaattgtta tgtagggact tacagaagcg gaatcaatac agtcaatagg atttatcgat
     2341 tagctttaga tgaaaaaaaa cctgtaccaa agcactggca aaagctcaaa gaattagtac
     2401 tgaattcgtc atggcgaaat tttgctttac agatttttga aagtcaggaa catttaaatg
     2461 ttcctgtttc gatgccagaa aaaatggcgg aagagcttta tggaaaagag atttatactt
     2521 caatttcacg tatggagaat ttttataatt gtgaatacaa atacttcatg caatttggtt
     2581 tgaaattgaa agaacgaact gtttatggtc taactccagc agcaactggt gatttttatc
     2641 atgaatcatt ggatcgtttc ttcaagctac tgttttcaaa ccaattatcc ttagtgaata
     2701 tgtccgatgc gcagcgtaga gactttactg aaaaagtgct gcaggaagta ttcggagaac
     2761 ttcggtttga gatattggat agttctgcaa gaatgaacta tatccgctac cagttaggac
     2821 agacgattca aaaagtgaca tgggctttgc aaaaacagag caaaaataca ggcatgcatc
     2881 ctcttcagac agagattcta tttggtcaga ttgctggagc aaaaggtatc acaggtctgg
     2941 aattaccgtt gagtaatggt ggaaaactcc atgttcgagg gaaaatcgat cgaatcgatg
     3001 tagcttctga acaagaagat acttggctaa gcgtcgttga ttacaaatct agcggacgtt
     3061 cattcgatgt cacagaagct tattatggca tggctatgca attattgacg tatctggacg
     3121 tagcattgat ggatgcggta cgtttgactg gaaaggcggc tgttaaacca gcaggatcat
     3181 attatttaca tgttcataat cctgtattga cggagacaga tgatgtagag aagaatacat
     3241 tgaaaaaatt tagttatgat ggcgtttttg tcgatgatcc cgctctttta gaagtactag
     3301 atcattcact ggaagcaaaa gaacaatcac tgatctatcc agtgaaaaaa aatgcgaaag
     3361 atgaatacca aaaaggtagt ttcagtaaag acaaattttt cacggaagaa gaactgcaac
     3421 tattcatggc gcataatcgg gaaaatatgc ggttagctgg agatgcgatt acttccggag
     3481 aaatcaaact aaatccagct tataaagata aacagcggat tgcctgcgaa ttttgtccat
     3541 tcagaagcat ctgtacgttt gatgtgatgc tgaaagaaaa taattaccac cgtctggaga
     3601 aaatagataa ggaagaagca ctgcgtagaa tattgaaaag gagtgaagaa gagtgaatca
     3661 aattccttta aaaccgcaaa atgaacgatt cacagatgat cagtggcaag cgatttttga
     3721 tcaaggagat aatctattag tctctgcgtc tgccggatca ggtaaaacta cagtgcttgt
     3781 tcgaagagtg attgaaaaac taaaaatggg ttttgatatc gatgaattat tgattgtgac
     3841 atttactgaa gctgccgcaa gagaaatgaa agaaagaatt caagaggcat tacaagaatc
     3901 cgtcaatagc gaaagtgacc ctgtacgtag acagcatttt actaagcagt tagttttatt
     3961 gcccacagcg aatatctcaa ccttacatgc tttttgcctg acagttattc gacgttatta
     4021 ttacttaatt gatattgatc cagtctttcg catgttaaca gatgaaacag aaacgatctt
     4081 gatgaaggag gatgtgtggg acgagctgag agaagcactt tatgcagaaa atgatgaacg
     4141 atttttccaa ttaacaatga atttttctaa tgatcgttca gatgacggct tgacgaatct
     4201 ggtgttttct ctttatgagt ttgcccgtgc caaccctgac ccccaaaaat ggctagaaca
     4261 gttaagcgat aattatcgtt tgcctgaggg gctagcgaaa tctcgattat atcaaaaaca
     4321 aatccgtcct ttagttttgg cagatatata ccaatgtgta cagttatatg aacagatgac
     4381 tcagttagca caaggtgaag gattagaaaa aatgcatgag caagtagctg gagaacaaca
     4441 gcagatcaag aatatctatg aagcgttctc gcaagatcgg ttagaagaag cttatgctgg
     4501 tttggaacaa ctaacttttt caacttttaa gagcagtcgg aaagcagaat tgaaagaaat
     4561 ttccaatgaa gtcaagggca tgagggataa agcaaaaaaa ctgattcagc agataagtaa
     4621 aagttatttt cctgtttccc caagccaaat ggaagaatta acggacaaag cactaccgtt
     4681 agtcgaagaa atgacaaaag tcacgcaaag cttcatggac ggtttcagta tgcgtaaacg
     4741 ggaaaaaggt gtgctggatt tcaatgatct ggaacattta gcgttgcaga ttctcacaga
     4801 aaaaacgaaa gatgtatggc tgccaagtga agcttcaaaa cattatcgaa aaaaattcaa
     4861 agaagtaatg gtagatgaat atcaagacgt gaaccaatta caagaagcca ttttatactg
     4921 gctaagagaa cccgatgata caaaaggaaa tatgttcatg gttggggatg tcaaacaatc
     4981 catctattct ttccgtttag cagatcctag tttatttatc gggaaatacg agaatttctc
     5041 aaagaaagaa ggcggacgtc gaatcgtttt ggctgagaac ttccgctcta gaaaagaagt
     5101 cctttctttt acgaatctta tttttgaaca actaatggat ccagcagtgg gtcaaatcaa
     5161 ttatgacgaa gcagccaaac tgatccaagg attttcagac tttccagaga atgaacagtt
     5221 tgagccggaa atcatgatct atgaaaaaga acaggaagaa tcagagattg agattccgac
     5281 ggatgatata ctggaagaca aaacagaagg cgaacttttt atgacaggac taaaaattcg
     5341 tcaattaatt gattcctcct ttatgattta tgataaaaaa tcaaaaaaaa gtcgtccgat
     5401 agagtacaaa gacattgttc tcttgacacc tacaaagaag aacaacttaa cgattttaga
     5461 gattttcaaa acattggata ttccattaga gatgaatgat gctcaaaact attttcaagc
     5521 gacagaaatt cgcacgatga tctcgctgct acaattaatc gataatccct atcaagatat
     5581 tccgcttgcg gctgtattgc gctcaccgat cgtgggtctg attgaaccag aattagccag
     5641 cattcgatta gctaaccgtg cacatactta ctatgatgct gtgcttgctt atcaagcatc
     5701 aaacgaagat gaattagcag ctaagctgga acattttggc aagcaactag agcattggcg
     5761 agaacttgct cgccgttctt cgatcactga cttgttatgg gatatctatt atgagacggg
     5821 atatctagag tatgtggtcg gtctgccagc aggggcacag agacaagcca atctttatgc
     5881 attggttgat cgtgccaaag cttacgagca aagttctttc agaggattat accagttcgt
     5941 gcgtttcatt gaaaaaatgc aagaaaaaga taaagactta gcagaaccag tcatttcaat
     6001 agaagataat gctgttcgcg ttatgacgat ccatgcaagt aaaggcttgg aattcccagt
     6061 tgtatttttg ttagatatga caaaggagtt caacttacaa gacttgcgca atcggtatgc
     6121 ttttgaagaa aaacttggag caggaattcg ctatatggat cctgaaacac gagtgttata
     6181 tgatacgtta ccgtttcaag ccatcaaatt ggcaaagcaa aacaaattgt tgtcggaaga
     6241 aatgcgtaaa ttgtatgtag gattgactcg ggcagaacaa aagcttttca ttgtcggttc
     6301 ctataaaaat aaagagcaaa tgatccaaac gtggtctgaa gcagctgatc atgaggagtt
     6361 agtttttgat cctgcgctac gtttgaaggg cagaagcagc ttgatgaatt ggattggtta
     6421 cggattgatc cgtcatcctg aaatgcagaa atacttagag gaagaaattt cgacttctct
     6481 tcttcaacat tcaaatgcac agttttctat ttcatggatg aatcagcaat cgatcattga
     6541 acaaagacag ttgcttgctg aaaaagaact ggttaactta gatcagcaga tgaaagaaga
     6601 tgaaacttta ttagcagatt cgttacaaaa acgtctggca tatgaatatc cttatcaagc
     6661 ttcttcacaa accacaagtt atcaatctgt atctgaaatc aaacgattat tcgaagatcc
     6721 agatgataca caagaatcac gtttgactct ggaaagcagt cagaacaaag cagcatcccg
     6781 tcaatttcgt tatacgcaag aacaactagc agaaccaaaa tttttgcaaa aagatcggca
     6841 agtgtcagca gctgctgttg gaactgctac gcatgctttg ttacaattac tgccattaga
     6901 gatgccaact acagagagta tccatcaaaa attacaggaa ttggtaagga aacggttagt
     6961 agacgaaaaa gtcgcgaaaa aagtagatgt ttcttctatt atatggttct ttcaaacaga
     7021 acttggtcaa cagctgattg ccaacaaaga aaacgtaaaa cgagaacaac ctttttccat
     7081 gctgcttcct gctgacgaag tatttcaaga ctatccaaat caagaagatg aactattaat
     7141 ccacggaatc gtcgatggat acctagaaga aaaagatcat ctcaatatct atgattttaa
     7201 aacagatttt attttacatc cggatgatcc agcagaaata gatgcaatcg tccaaaaata
     7261 tcaaggacag ctaagacttt accaacaagc aatgtcagaa gctttgaata agccagtaga
     7321 aaatgttttc ctaattttgc ttcgagtgaa acaaattata aacattaaca aataattata
     7381 attgagcaaa cttgacttat gttttgtaag gattatgcta tactctggga agaggtgagt
     7441 atgatggagc taacacagac agaattcgtt ttatgcccta aatttgaaaa aacattctct
     7501 attttaggaa aaaaatggaa cggattgatt atcgatgtac tactcgaaaa cggtccgcaa
     7561 cgctttgttg agcttgcggc aaaaatcccg atggtcagtg atcgagtatt agtcgaacgc
     7621 ttgaaggaat tagaaaaaga aggaatagtc actcgaaact ttgcctgtag cgaaaagagc
     7681 cgttcagaat atctcttgac tgaaaaagga gcagacttgc aaaaagcgat gcgtcaaata
     7741 caagtttgga gcgaaaaatg gatcacagag gaagaatgcg gttgattttg taaaaaaaat
     7801 cttgtaaact atagtccata cgaataagta aagaacgatg acgaaaaaga gtagtttggc
     7861 taaaccagtt cagggagatc tgtcgcagac tgaaagcaga tcctgtgttt gaaaaatgaa
     7921 gttcactttc cgagttcgtc tgataaaatc agaccggcaa ttgtcgttaa cgaagtaaga
     7981 gtgcggtgta agtcatttat ccgcaaatta ggatggtaac gcgaagacct cgttccttaa
     8041 caggaatgag gtctttttgt tttgtatttg gaatttaaag gaggaacaag cacatgtctc
     8101 tacaagcaca attagagagc ttgaaagaag caacgctcgt caaaatcaca gcggcacaag
     8161 atttaaatga attaaatcaa atacgtgttg aaacgttagg aaaaaaagga ccgatcacag
     8221 aagtattacg tgggatgaaa gatctaagtc cagaagagcg accaaaagtc ggaagtttcg
     8281 caaatgagat ccgagatctt ttgacagaag agatcgagcg aaaaaaacaa gtattagaag
     8341 aagaagcgtt aaatgcagct ttggcaaaag aaacaataga cgtgacacta cctggtagaa
     8401 aagtaaaaca tggtacacgt catatcgtga cacaaatcat ggaagaaatt gaagatattt
     8461 ttgtgggtat gggctatcag atcgtagaag gatatgaagt agaatctgat cattacaatt
     8521 tcgaacggat gaatctgcca aaagatcatc cagcaaggga catgcaagat actttctata
     8581 tttcagaaga gatattgatt cggacacata cttcaccagt ccaagcacgg acaatggaaa
     8641 aacatgattt ctctaagggt gctctacgaa tgatctcgcc agggaaagtc ttccgcagag
     8701 atacagatga tgcgacccac agtcatcagt tccatcaaat tgaagggcta gttgttgata
     8761 aaaacatcac aatgggcgac cttaaaggaa cattagaagt cgtaatgaaa aaaatgtttg
     8821 gggaagatcg tgaaattcgt ttgcgtccaa gttatttccc atttacggaa ccatcagtag
     8881 aagtagatgt cagctgtttc aaatgtggtg gtgccggttg taacgtatgt aaatacactg
     8941 gatggatcga aattttagga gctggcatgg tacacccgaa tgtgttgaag atgtcaggaa
     9001 tcgatccaga agaatattca ggctttgctt tcggccttgg accagaccgc gtagctatgt
     9061 tgcggtatgg agtgaatgat atccgtaatt tctatcaaaa tgacttacgt ttcttaagtc
     9121 agttcaaggg ggagtaagaa aatgttagtt tcatataaat ggctcaatcg ttatgtagat
     9181 ttatcgaatg taacaccaaa agaattagcg gataagatgt ctgtgacagg aatcgaagta
     9241 gagggagtaa cggttccaga agaaggcttg aaaaaaatcg ttgtcggtga agtcaaggaa
     9301 tgcgtaccac atccagattc tgatcacctt tctatttgtc aagtcgatat tggcgaagac
     9361 gaactgtctc aaatcgtttg tggagcgcct aatgtgaagg ttggaatcaa agtgattgtt
     9421 gcacttccta actcacgtat cgctggtaat gtaaaaatca aaaaaggaaa aatgcgtggc
     9481 caagtatcaa atggcatgat ttgctcatta caagaaattg gctattcaga tagtgttata
     9541 ccgaaagaat acgctgaagg tatctattat atgccacaag aagctgtaaa tggcgatcca
     9601 gtttttcctt atttggatat ggatgattcg attattgaat tatctatcac accgaatcgt
     9661 gcggatgcat tgtcaatgcg tggcgtagct tatgaagttg gggcaattta tcgtcagact
     9721 cctacattcg atgacgaaaa gctggtagaa tcctcacgtc ctgctagcga taaaattcaa
     9781 gtggaagttg aagataaaga agcagttcct gcttatcaaa tcagaatcat tgaaggagta
     9841 agaatccagc caagtcctca atggctgcaa aatcttttga tgaacgaagg cattcgtcca
     9901 atcaacaatg tagtagatgt gacgaactat attttgcttt tattcggaca accactacat
     9961 gcatttgact acgacaaatt gaaatcaaaa gagatctttg tccgccgtgc aaaagaaaat
    10021 gaaacattag taacactaga tggagaatct cgggaattga caactgacaa tctcgtcatt
    10081 actaacggca aagaaccagt tgctttagct ggtgtcatgg gagggttaga ctcagagatc
    10141 acgaatgaaa caacaacagt tgcactagaa tctgctttat ttgatccaat ttcgattcgt
    10201 cgtacagcaa aacaatttaa tttgcgtagt gaatcctctg cacgttttga aaaaggaatc
    10261 aaccatgcga ctgttggaga agccagtgag gttgctgcag caatgatcgt ttctcttgcg
    10321 ggtggggaag ttctacaagg cgcggtcaaa ggctcagaga tcaaagcaga agacgtatca
    10381 gtgaagattt cacttgaacg catcaatcaa tatttaggaa catctcttgt cgttaaagaa
    10441 gtaaatgaga tttttgaagc acttggattt agctatgaag aaaaggagta tgtttattcc
    10501 gtagtagtac caccaagacg ctgggatatc caaatcgaag cagatattat cgaagaagtt
    10561 gctcgtattt atggatacga ccagttgcca tctacacttc caagtggaga aacggttgct
    10621 ggaagcttgt caaaagaaca agaaactact cgtaaaattc gtacaatctt tgaaggaagc
    10681 ggattgagcg aagcaatcag ttatgcattg acaacagaag aaaaatcaag acagtttgtt
    10741 ataaatgaaa gtaatattac acgtttggac tggccaatga gcgaagaacg ttctgtatta
    10801 cgaatgaatt tgatcagcgg actattagat gatatcagtt ataacactgc ccgaaaaaat
    10861 acagagattg ctttatatga aattggtcgg gtgttcttcc aagaaaatga tccacgtaca
    10921 catctgccgc aagaagtaaa acatgcagca atcgctattt ctggtatttg ggaagaaaaa
    10981 gattggcaga cgaaagctca aaatgtagac tttttcacca tcaagggtct tttagaaaac
    11041 ttatttgaac aactaggcat cactgaagat gtccattatc aaacagtgac agaaatgaag
    11101 gaaatgcatc ctggaagaac tgctgcgatt taccttggcg aaaaatttat tggttttgtc
    11161 ggtcaagtgc atccaacgat tacaaaaagt tatgatattc cagaaacata tgttgctgag
    11221 tttgatttat ctgctatagt ggaagctgct caaaaaggga tcgttttcga agcagtgaca
    11281 aaattcccag cagtcagccg ggacatcgct ttgcttgtaa aagaaactgt gacaaatcag
    11341 gaattaacag aagttatccg ttctgctgcc ggaagatttt taacagatat ccaattattt
    11401 gatgtctatc aaggagaaaa cattgaaaaa ggacataaat ccatggcgta tagcctgaca
    11461 ttcgtcaacc cagaagcaac attgacagat gaagaaatca ataaagcaat ggaaaaagtc
    11521 gaaaaagcgt tggtagaaaa cctagaggca agtattcgct aagaaggaaa atatagtaag
    11581 taacgatcaa gtagcttttg aagaccatac atttgaaaaa aagatgtgac acaacttcgt
    11641 cacatctctt tttttgattt taaaaaagga atagcacaga gacgagataa aaaaacaggc
    11701 ctttacagcc tgctttttta gagtaaatga attaatggaa gtaattgatt cccattgctg
    11761 attttacttc agaaagtgtt tgtgcagcta ctttagcagc ttcttcacta cctttacgta
    11821 agatttccat aacagctgaa ggatcttttt ccaattcttc tcggcgcgca cggatcggtg
    11881 caaattcagc ttccaataca tcaatgaggt atcgtttgat ctttacatca ccaagtccgc
    11941 cgcggcggta atgagctttc atttcttcaa ttgcttcttt atctgttccg aatacatcca
    12001 agtaggtgaa gaccatattc ccttctactt gtccaggatc ttgtacatgg atgtggtttg
    12061 gatcagtata catactcatg acttttttct gtaagacatc ggcaggatcg gagatataaa
    12121 taccgtttcc taacgattta ctcattttgc catttccatc gattccaggt aatctaccca
    12181 ttccttttgg tgggaatacg ccttttggtt ccaccaagac ttcaccatat gtatgattga
    12241 agctttgtac gatttcctgt gtctgctcta gcattggttt ttgatcctca ccaacaggta
    12301 caagattcgc tttgaatgca gtgatatctg ctgcttgaga aacaggatag ataaagaatc
    12361 ctgtaggcac gctttcaccg aatttttttt gttcgatttc tgttttgaca gtcggattgc
    12421 gccgaacgcg tcctacgctg actaagttca aataatacat ggttaattca gctaattccg
    12481 gaatttgtga ttggataaat aaagtagatt ttgcaggatc taatcccacg gctaaatagt
    12541 ctaaagcaac ttgcaagaca tttgatgaaa ccttctcagg atttttagca ttatctgtca
    12601 gtgcttgcat gtcagcaatc atgacaaaca gttggttgtc ttcatctgcc tgcatagcca
    12661 ctcgggtttt tagagaaccg acgtaatgcc ctaaatgcag cttcccagtt ggtcgatctc
    12721 ccgttaaaat aatatttttc aaaaaagaca cctctttacg atttgtaagt ctgagcactt
    12781 aatatcttac cgtttttcct gaaaagaaac aacagttcct aaggaaaaaa caaggaaaga
    12841 ggataaaata actaatagat tagaaatgga agaaatattt atgggagaat aaacgttcta
    12901 agaaacttta caaattacta aactatcaga tataatgtat tttattatac atctttaaaa
    12961 gaaatcttac taaataataa tgggagtgta attctatggc aatgattaaa tttgatcacg
    13021 tggataaata ttatggcaag ttccacgcat taaaaaatat caatctcgaa ttcgagaaag
    13081 gagaagttgt cgttgtcatt ggtccttctg gttcaggaaa gagcacgatg cttcgctgta
    13141 tcaatggatt agagacgatc tcttcaggta agctgctgat taatgatact gacttgcatg
    13201 ataaaaagac aaaactgaca gaagtccgaa aaaacatcgg catggttttc caacatttca
    13261 atttgtaccc aaataaaact gttctggaaa acatcaaatt agcaccaatc aaagtgttga
    13321 agcaagacca ggcaactgca gtcaaaaacg cggagaaatt tttgaaaact gtcaatatgt
    13381 tagataagaa agattcttat ccttccatgt tatccggggg acaacagcaa cgggtggcaa
    13441 tcgccagagg tttagcgatg aatcctgaga tgctgctgtt tgatgaacct acttctgctt
    13501 tggatccgga aatgattggt gatgtattag acgttatgaa aaaattggct catgacggta
    13561 tgtcaatgat tgtcgtgaca catgaaatgg gctttgccaa agaagtggcg gatcgtgtta
    13621 tcttcatggc tgatggagaa gtattggaag acagcagaaa tgtgagagac tttttcgaaa
    13681 atccaaatga agtccgagca cagcaattta tcagtaaagt tatcaatcat tgataggagg
    13741 aaaaagatgc gcaggttaaa aaaaatacgc ctgattttcc ttttaggcat tgcttttctt
    13801 gcactagccg gatgtaaagg aaatgggttg gcaaatgaag atattttaga gcgaagcaaa
    13861 tctaccaatg aaatcatctg gggtgtcaag tatgatactc gattatttgg aatgatggat
    13921 attgagagca gaactgtaca aggatttgat gtagatattg caaaagcaat cactaaaaag
    13981 atccttggtg acaatggaaa aacagaattt gttgaagtca cttctaaaac tcggattcca
    14041 ttattgaaaa acggaaatat cgatgcaatt attgcaacga tgacgatcac agatgaacga
    14101 aaaaaacagg ttgacttttc agatgtctat ttcgatgctg gccaagcttt gttggtaaag
    14161 aaaggaagcc aaatcaaaag tgtagatgat ttgaatgctt ctacaacggt tctagcagtc
    14221 aaaggttcta catcggctgc gaatatccga cagcatgcac cagatgcaaa aattttagaa
    14281 ttagaaaact atgcagaagc atttactgct ttacaatctg gtcagggaga cgcgatgact
    14341 acggataatg cgattttgtt agggattgca gatgaaaatc cagagtatga gttagttggt
    14401 ggaacgttta ctaatgagcc ttacgggatt gcgatcaaca aagggcaaga aaacttcttg
    14461 aaagctgtta accaagcgct agaggaaatg catgcagatg gcacatacga taaaatctat
    14521 caaaaatggt ttccaaatga aactgaagga aaagtagagt aagggggaga ggcaatgagc
    14581 gaattgatac aaacttatgg accaatcttt gtcgacgggt tcaagatcac attgttttcg
    14641 agtgtcttag cacttgtgtt tagtttgatt atcggaacgt taatggcaat cttccaatta
    14701 tcaaagaata aagtagtgag cgccttagca aaggcatatg tagaattttt tagaaacatt
    14761 ccattattaa tcatcgtgat gtttttttat gttgtcgtac cgatttattg gtttaatcta
    14821 gatggattcc aagcaggtac gattggatta acgatctata catctgcttt tattgctgaa
    14881 acagtacgtt cagggattca aaccgtacca aaaggtcaaa cagaagcagg actatcttct
    14941 ggttttacct attcagaaat catgcgttat attgttttac cgcaagcatt caaaatcgtt
    15001 atcccgccat taggcaacca atttatcaac ctcgtaaaaa actcatctat tttagcaatc
    15061 gtagctggtt tagatttgat gtatcaagga gacttgatcg ctagtgaaac attcaatacc
    15121 ttcgatacgt atatcatcgt tgggttattt tacttagtta tcacgctacc gttatcttac
    15181 ttaatggttt atcttgagaa aaaatgggcg gttcgttcat agaaggaggg gaaatacatg
    15241 gattttagcg gcgctttttc atggatgaat atccgttttt tattggaagg attaaaggtt
    15301 actgttgaag tttcattgtt ttcgatcatt tttagtttct tgatcggtgg tctgacaggt
    15361 gtgattcgtt ttgcgaatat ccctttcttt tccaaaatcc ttggtgtagt gattgatatt
    15421 atacggaatc ttccattatt attgatcatt ttcttcactt actttgcctt acctcaaatc
    15481 ggcattcaga tgaacatctt ctggtccgct gtcgcagcat taacgatttt tgagtcagct
    15541 atgttgtcgg aaattttccg agctggactc aatgctgttc caaaaggaca gatggaagca
    15601 ggattatcaa cgggactgac ttatgtagaa tcgatgcgaa cgatcgtgtt accgcaagca
    15661 tttaaatcaa tgatcccggc aattgtgagc caattgattt cactgatcaa agatacttca
    15721 ttagcagtaa tcatttcttt acctgaactt tctcatcagg cacgtattat ttatggacag
    15781 aacaccaatt atgtgcttcc gatgttcatc atcatgacac ttatgtactt cgtggtatgt
    15841 tacgcgcttt ctctgctttc cggttacttg gaaaagagac ggtacagtta ttaatagatg
    15901 attcttttag cggttgtggc aaactaaagt cacaaccgct tttcctcttt ccattcgaaa
    15961 aaaagagatt tcgattgttt taagcaagta agagcacttg aaaattcacg ctaaaaagta
    16021 cacaataggg aggatagaat ttattgaaaa aaaggatgat acgattgaca gataatcgcc
    16081 ctatcggatt tattgattca ggtgtcggcg gcttgactgt agtaaaagaa gccctgaaac
    16141 aattaccgaa tgaaaatatt ttatttgtag gagacacagc acgctgccca tatggcccta
    16201 gacccgcgga acaggtaatc cagtatactt gggaaatgac ggattatctg gtggagcaag
    16261 gaatcaagat gctggtgatc gcctgcaata ccgcaactgc ggtggcttta gaagaaatca
    16321 aagctgctct ttctattcca gtcatcggtg tgatccttcc cggtactaga gcggcagtaa
    16381 aaaaaacaca aaataaacaa gttggcatta tcggtacgat tggtacggta aaaagtcaag
    16441 cttatgaaaa agcactgaaa gagaaagtac cagaattgac tgtgacaagt cttgcttgtc
    16501 ccaaatttgt ttcagttgtc gaaagtaatg aataccattc atcggtggcg aaaaaaattg
    16561 tggcagaaac attagctcct ttaaccacta aaaaaatcga tacattgatt ttgggatgca
    16621 cccattatcc attattacgc cccatcattc aaaatgtaat gggagaaaat gttcaactga
    16681 tcgattctgg agcagaaaca gtaggtgaag tatctatgct gttagattat ttcaatttga
    16741 gcaattcacc gcaaaatggt cggacattat gccagtttta tacaactggc tctgccaaac
    16801 ttttcgagga aatagctgaa gactggcttg gaatcggaca cttaaatgta gaacatatcg
    16861 aattgggagg aaaataagga tgcgtcatga cgggagaaaa gcccaagagc ttcgcaaact
    16921 ggagcttcaa acaaatgttt tcaaatatcc agaaggatcg gtagtgatcc gttttggtga
    16981 tacaacagtt atttgttctg caacaataga aaattctgtt cccccttttt tgagagaaac
    17041 aggaacagga tgggtgacag cagaatatag tatgctgcct cgagcaacga gtacgagaaa
    17101 tcgtagagaa agcagcaaag ggaaactttc tggtagaaca atggaaatcc agcgtcttat
    17161 tggtcgttcc ttgcgtgcag tagtagactt ggaaaaatta ggagaacgta gtatcatcgt
    17221 agactgcgat gtgatccaag cggatggcgg tactagaaca gcaagtatca ctggggcatt
    17281 tgtggcattg aaattagcca ttgaaaaact tttaagagaa aaagaattgt cagaagatcc
    17341 aataaaagaa catcttgctg cagttagcgt aggaatctta ccagatggga cttgcgtcac
    17401 agatttggat taccaagaag attccgcagc acttgtagac atgaatcttg taatgacaga
    17461 atctggaaaa ttcgtggaga ttcaaggaac tggagaagaa gcaacgtttg atggtgaaca
    17521 gttgaatgaa atgctctttt ttggaaaaaa cgcgattgaa gatttgatca aagagcaaaa
    17581 acatgctttg ctaacagagt ttgctcaaaa tgatgagcgg atagaagaaa caaaaacaat
    17641 catcattgcg acaagaaatc caggaaaagc agaagagttt cgaaatatgt tcaaagaagc
    17701 gggatatcat gtaaaaacat tacttgacta tcctgaattg ccagatgtgg aagaaactgg
    17761 cagcacattt gaagaaaatg cccgattaaa agcagaaacg attgcacaat tgcttgatca
    17821 gccagtctta gcagatgatt caggtttgaa agtggatgct ttaggcggta tgccaggaat
    17881 ctattctgca cgttttgcag gggaacaaaa aagtgatgcg gggaataacg ccaaattatt
    17941 atatgaattg acagatgtgc cggatgaaaa aagaacagca caattccatt gtacgttagt
    18001 attcgcggca cccaaaaaag acagccttgt agtagaagcg gaatggcccg gtcgagtagc
    18061 ccgtatacca tctggcgaaa atggctttgg gtatgatcct ttatttatcc cagaaggtaa
    18121 gaaacaaaca gcagcagaat tatcgagcga agaaaaaaac aagatcagtc atcgggctca
    18181 agcaatgaaa aaattgagtg ccgagtggaa acaatggctg gaaggagaga gatagatgcg
    18241 gtatttagta gtaagtgata atcatggaga cagagatatc ctcgttgact tagtagatcg
    18301 ttatcgttct gaagtggata tctttttcca ttgtggtgat tcggaattag aagcttctga
    18361 cccattatgg gatacattcc aagtggttca aggtaattgt gattatggac caggattcga
    18421 acagaaaaaa gtgatccaaa ctggtcagga tacagtattc atgactcacg gtcatttatc
    18481 gaatgtccga tttggtttga cacagttagc aatcgaagct cgagctgcaa ctgccgatat
    18541 ggtgtttttt ggtcataccc atcaaatagg ctgtgagatg gatcagaaaa tcctgttttt
    18601 gaatcctggt agcatttctc agcctagagg cccgatccaa atcccatcat atgctcttat
    18661 cgaaagttta gatgaccaaa tttctgttca atactataac cgtgcccatc aggcagttga
    18721 agagttggcc ttttcttttt cgagagagcg ctgaataaaa aaactctaat ttttaataaa
    18781 aataagcttt attttatgaa ttttgcatcc ggtttcggta aaatagtagt gttgaatgaa
    18841 atagaaatgg aggccatcag atgattggac ctatagtaag agaaatgctg ctggaaaatc
    18901 aggaaacgtt tttgattcct gcagaaaatg tggcaaatgt tatgtatcat catccattgt
    18961 cccatggttt acttgtgtta tcgaaggtcg gttattccaa aattcctgta ttaggaaaag
    19021 atgatcactt tgtaggacta gtaagtttat cagatgtggt caataaaatg attgacttgc
    19081 aaacaatcag catggacccg ttagaaggac taacggttgc tgatgtgaca gaaacagatg
    19141 ttccgacaat tgatgaaaat tgggaactag aagaagtact ccatttgtta gtcgattcgc
    19201 cttttttacc ggttgtcaca gaagatcgga ttttcaaagg aatcattaca agaaaagagc
    19261 tgcttaaagc cgtaaacttc atggtgcatg aattagaacg tcgtaatata ttgtctccga
    19321 aaatcgaaga attaaaagaa caaatcgata tcgtgagcta aagaacgaga aactaaaaga
    19381 taatcaaaaa ataagtagaa aaaacagcgt accagttttg actttatctt gaaaaagagg
    19441 ctgggacaaa agcgtttaac tccaagaaat aagaag
//
LOCUS       DACDYJ010000047        19475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDYJ010000047 DACDYJ010000000
VERSION     DACDYJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.047"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            105..1148
                     /locus_tag="IWM51_001807"
     CDS             105..1148
                     /locus_tag="IWM51_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAP9971229.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            1321..1545
                     /locus_tag="IWM51_001808"
     CDS             1321..1545
                     /locus_tag="IWM51_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971230.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            1548..1805
                     /locus_tag="IWM51_001809"
     CDS             1548..1805
                     /locus_tag="IWM51_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971231.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            2022..2897
                     /locus_tag="IWM51_001810"
     CDS             2022..2897
                     /locus_tag="IWM51_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971232.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            2907..3701
                     /locus_tag="IWM51_001811"
     CDS             2907..3701
                     /locus_tag="IWM51_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971233.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            3730..4848
                     /locus_tag="IWM51_001812"
     CDS             3730..4848
                     /locus_tag="IWM51_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971234.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            4881..5201
                     /locus_tag="IWM51_001813"
     CDS             4881..5201
                     /locus_tag="IWM51_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971235.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            5456..6145
                     /locus_tag="IWM51_001814"
     CDS             5456..6145
                     /locus_tag="IWM51_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HAP9971236.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            6146..6349
                     /locus_tag="IWM51_001815"
     CDS             6146..6349
                     /locus_tag="IWM51_001815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971237.1"
                     /translation="MSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNA
                     YIARLKRFIDKYKENSDKFSKDK"
     gene            6369..7997
                     /locus_tag="IWM51_001816"
     CDS             6369..7997
                     /locus_tag="IWM51_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971238.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            8048..8419
                     /locus_tag="IWM51_001817"
     CDS             8048..8419
                     /locus_tag="IWM51_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971239.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            8504..8704
                     /locus_tag="IWM51_001818"
     CDS             8504..8704
                     /locus_tag="IWM51_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971240.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            8838..10064
                     /locus_tag="IWM51_001819"
     CDS             8838..10064
                     /locus_tag="IWM51_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971241.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            10914..12238
                     /locus_tag="IWM51_001820"
                     /pseudo
     CDS             10914..12238
                     /locus_tag="IWM51_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            12231..12578
                     /locus_tag="IWM51_001821"
     CDS             12231..12578
                     /locus_tag="IWM51_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971242.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            12929..13750
                     /locus_tag="IWM51_001822"
     CDS             12929..13750
                     /locus_tag="IWM51_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP9971243.1"
                     /translation="MGLLVPRANTYTIPLTKRQLIAKRYQRITRAINREFWNSESDTA
                     HSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIKNAFQEILPN
                     SDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMGGNWKATNPKKEQ
                     EAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSG
                     GQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            13770..14330
                     /locus_tag="IWM51_001823"
     CDS             13770..14330
                     /locus_tag="IWM51_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HAP9971244.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            15226..15993
                     /locus_tag="IWM51_001824"
     CDS             15226..15993
                     /locus_tag="IWM51_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP9971245.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            16019..16564
                     /locus_tag="IWM51_001825"
     CDS             16019..16564
                     /locus_tag="IWM51_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971246.1"
                     /translation="MGERTQALITVKYGNSKTKVSVSIHYQWGYGRFMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            16565..17131
                     /locus_tag="IWM51_001826"
     CDS             16565..17131
                     /locus_tag="IWM51_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971247.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTIEGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            17146..17394
                     /locus_tag="IWM51_001827"
     CDS             17146..17394
                     /locus_tag="IWM51_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971248.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            17413..17652
                     /locus_tag="IWM51_001828"
     CDS             17413..17652
                     /locus_tag="IWM51_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971249.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            17669..18253
                     /locus_tag="IWM51_001829"
     CDS             17669..18253
                     /locus_tag="IWM51_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971250.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            18360..18641
                     /locus_tag="IWM51_001830"
     CDS             18360..18641
                     /locus_tag="IWM51_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP9971251.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            18631..18972
                     /locus_tag="IWM51_001831"
     CDS             18631..18972
                     /locus_tag="IWM51_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9971252.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         7480 a         2455 c         3922 g         5618 t
ORIGIN      
        1 acaacaacga ttatgatctt aaaacaatcg tttatgtaat tggcaatctt acgaataatt
       61 ttcatgcaat aatgacaata aaaaatgaaa aggggtaaaa tcaaatgaaa aaatttttag
      121 taggaattct attcacaatg gggctagtat tattatcacc ttcagtttca gcagaaagct
      181 ctatttccat gtatcgtatg tataatccca atagcggaga gcatctatac actagaagcg
      241 ttggagaaag agacaatctg aaacgttatg gatggcatta cgaaggtgtc gcatggcaag
      301 ctccaacaag tggtactcct gtttatcgtt tgtataatcg gtataacggt gaacattttt
      361 acacagttga tgccggagaa agagataaga tttcacacac aggatggacg tacgaaggtg
      421 ttgcgttcta ttcctacaac ggagcaaata gagttcctgt aacacgttta tataacaaat
      481 acgtgaattg gcatcactac acgttagatc aaaatgaaaa gaacgtaatt agtaaacaag
      541 gttggaagat tgaaggtaca ggctggtatg caatgccaaa cagcgtacca aatcctacac
      601 ctgctccaag cactgaaaaa gtgttgaacg ttcctgttgt atggcaagga aatacaatgt
      661 tatgcgaagg agcttcttta ttagaaggtt tacactacaa aggtgtagcc acaaatcaaa
      721 gcttaaccag ttttgtaaac tatatgccaa aagctaatga caataatcct tatcatggtt
      781 actcaggaga gtggcgtcat aacgttaatg gaacgtatca aggcatgatg gctgacccag
      841 tagttaaatg ggcaaaaaac gttggcggta atgccgtcaa tatcactggt tcaggaacga
      901 atggaatcaa gaatgaaatt cgcaatggta atccggttgt aacttggatt acttataact
      961 atgctacgcc agagttcaaa caaatgcctt ggggtcgtgc agtatggaat ggacatgtag
     1021 ttctcgtgga tggcttcaga aatggtgcat accatgttgt tgaccctgta tttggcgcaa
     1081 aatggatcaa tgcaagcacg tatgagagag catttaatgt gactggaatg gcggttgctg
     1141 tacgttaatt gatagaatta tcaactaaat gaaataagag cttaattact gaaatggtaa
     1201 ataggctctt atttttttag aggaaatttt tgtaatacga atctataaca aaaaatattg
     1261 aatgtagtac acaaaagtgg tatattaaaa tcgtagagat tatgcgaaga ggtgattttg
     1321 atgaaccaaa gaagaagaaa aaggttagaa gaaataagcg agcgactttc cgagctaaag
     1381 gatgagttag aagtaattca agaagaagag tacaaagaaa atatacctaa aaatctacag
     1441 ggatcagaaa gatatgaaaa agcagaagaa tccgttgatc aacttgaaga agctgttgat
     1501 ggtttatccg acatattaga attgattgaa gatatagcta gctagagatg attaatgaag
     1561 atttcaggag tgagaataaa atgattgata tttcactgat agagaagcta cttttttcaa
     1621 ataagtacac agccaaagag ttggctacaa gatggcaaat atccgaatca gctattaaag
     1681 cctatcgtca aaataaggga agcgtaggat ggcgtgattg gaaaggaatc agcattggaa
     1741 aagcagaaga cataatgaaa cgtgaaggat ttcaagctga caatgattct aaaaaagttg
     1801 aatagtttaa tagtcgccaa gaaaagtacc aggacgaggg gaaaggtaga ttacaaagga
     1861 aaacactttt aaataaactt cgcaaaagca tcaggttatg agtggctagg gctatacgaa
     1921 tgtgatgaaa acaaagggct ttaatgtcaa agaaaacgca ataggggggg aacaaaattt
     1981 ttaaaaaaga tatttatgtt tggtttaatg gaaaaaaata tatgactact aacaaaaggt
     2041 ggtcagagct agtacaaaat acagttaaaa aagtcatgcc agatggatat catttcaact
     2101 atatacaatg ctcacaaaca actgaaagta tttatttttc aatattaaat gagaatagac
     2161 tctattattt cagggtaagt tcacaccata ggaaaaagag agaagataat gttacgacat
     2221 ttttatcggg aaaatacaat ggcttcaaag agttaagtga agccattggt gagtatctaa
     2281 aaagtaatag atttgtcaga atttctgaca aacatatatt gatcttgtta ctttatttga
     2341 ataatagctt caaattgttt tggagagacg acactttcta ctatattgag aacggcatgg
     2401 atatttattc tggtatcaac ggcatattaa aacattatat taaggcgtta cttcgatatg
     2461 gggttcttta tcaagaagat ttaagtaacc aaattaagat tagtaattct ggaaagtcat
     2521 tactggcaaa atataaaaaa ttgctttctc ctatctacaa aggaatagat atggaagctg
     2581 taaattctga aactttattt aatttagttg ttgatgaaaa gtttgcgaat gaatttttta
     2641 aaagtaaaaa aattgaaaga ctaaataacc tgtttcctga aaatgcaata aaagcttatg
     2701 tagataataa taaatcacga ttatacaact ggtttattcc caaacggttg cgaggaaaaa
     2761 tcaaaattgg cgatcaagtg aaagtaatta atctaaatca tactaagatc gttaccgtaa
     2821 cggagttgta tatagctgaa aaagaagata ttacaaaaat gaaaccagtt attaaaatac
     2881 tagataacaa agagtgagtg gaatatatgg tagcaaaaaa gaatactcta aaagaagctt
     2941 ctgaaaaata tcaagatatg aaaagacgag gattaatacc aaaggacaaa aaaggtatat
     3001 ggagagaatt tgttaaggaa gtcagtagtg atcgaagtgg cggtgacttt gttggacgat
     3061 atacagcaaa aggttatagc aggcgcttaa ggatcactgt tgaatatgga acgaatgaat
     3121 cagctacaga gctgaagcaa aggtacttag atacacgaag agaagcttca aaaaaatatg
     3181 atgatttcaa gagcagaggt ctaattccta agaagaaaaa ggggatttgg cgtgagtttt
     3241 tcaaagaaga agatagcaat cggaataagg gagacttcat tggacgatac acagcaaaag
     3301 gctacactaa gaatttaggg atcaaggttg aatacggaac tggtgaaagt tcatgggaat
     3361 tgagaaatcg ctatttaaat acattgttag agaaatataa agcagaatta ttggagttgt
     3421 tcgatgttaa tgattttatc gagatggctg aaaagtatgg tcatgaagaa ggttttatga
     3481 tcaatgacat tatggttatt gataatttta cggatttatt agcacttgcg aaaaaagcaa
     3541 gggatttaag agaaacgcag atgtatctat tcagaaataa aaacggaaga aaccggagaa
     3601 ctgaatcgag tgagattgaa gattactatg atgcctttac acacgatctg ggagcctttg
     3661 tagacgagat aagtggtgta atgaacaaat atagggtcta gaataaaaaa gccggagatg
     3721 agcagataaa tgggaaacaa tagcatatac acacctgaaa tgcagaataa aattaaaaga
     3781 cttgtcggat tagggtggat gacaagagga aaattagatt cttttagtac gatcaaatat
     3841 cttttgatgg acatttaccg tcagaacaag caaacaggaa tacatttttt gggctatgat
     3901 aagccggaag atgcaccttt ctttgttcca agttcagcct atagagccag tacctccggt
     3961 agcatgtata acttggattt gtttgaggaa ggagcaaaga tgatcgaata tgatttcaac
     4021 gaagcctatt cgaggatcat gagaaattat cctttgccgt caaacactta tttgaaagca
     4081 aaatataata cagatcaagc tattttgaga gcgataaata gaccaaaagt aagaccgtat
     4141 gaaaatttaa aaacgtatat gttctttttg gttaattttg aaggaacagc caaagaagga
     4201 acatatgcta atccaaaatc acagtcgatg tttgcggatt atggcgaaaa tcccaaaaca
     4261 gctaatgagc ctatttggtt atcggagatt gagctaacac ttatccttga ttattatgat
     4321 attaaagaga tggaagtcct agatagccac acatttttga cgaaaaaagg attactcaaa
     4381 gaatattttg aaagaatcga gccattaaaa actgatcctg atacgttgaa gctttataaa
     4441 aatcttagaa cgaaagtatt tggtgctatc ggacaaaaat cattaagaga aggagaaaca
     4501 agcaccttcg ataaatacag tatgtataat cgagcgtatt cctccatggt agcaggcgta
     4561 tttagagatc gtatggttag atatgaacag aaatacgttc atagcgcata tgggctatta
     4621 aaaattcgta ctgatggatt gtatttcaga tccgaagtac cagagtttga agagctgtat
     4681 aaacttggag tagttaagaa aaaagttcat acaatatcca aagaagaagt cgaaaaagct
     4741 ttagaattta aaaacaaagg atttgaaaat aagggctcct ttgggaaatc taaagatgaa
     4801 gttaagggtg ttgtgataga cgatgatttc gagtttgatt ttgactaaat actctttgga
     4861 aaagatggta ggaggtaaat atgaagaaga aaattgtgga aggaaatgta ggtgttatta
     4921 ttctaaaaga ttcaactgac tatgctaaat caatggcact aaaagacgaa aaagttgcac
     4981 gcatggataa agatttagtt gaatttctga tggatgatac agaaagatta aaaggggatc
     5041 agtttttgag tttaatgaaa gacaaaggct taaacacata tggtttattt gattataaga
     5101 aattagctat agagtgggta aaagaaggag aaatattttt tattgaagaa tgggaaggta
     5161 ctgagaactt agttcttcat cagtatcttt tgaaagctta atctaagtaa aacagttctg
     5221 actagttatg cttgtcagaa tttcaaagga agttttccat aaccatcatc attcaaaaat
     5281 aaaggatttg aaaataaggg ttcctttggg aaatctaaag atgaagttaa gggtgttgtg
     5341 atagacgatg atttcaagtt tgattttgac tagatttttt tgcaatgttt aagagtaaac
     5401 aaggtaagaa ctaagatcat gaagattgag agtaaagaag atagaggacg aatgtatgag
     5461 aacacactat atttatttag aagataaaat tgtagatgaa attccggcaa aaagtcgaga
     5521 acaagctaga aaattattta ttgaaaaata caaagagatc aatggagata gtgaagttcc
     5581 aaaagtggtc gtaacagtta gaaaaaggca aacaaaaaag agaagtacgt ttcatgtcag
     5641 ccctgatata tataatctta tagagcgaca tagaagtgaa gataatattt ctatctctca
     5701 atatattgct acagttattg atgaattgat ttcaggaaag tatgactatg cttttgattt
     5761 tgagggtgaa aaggattaca aaagtgttgc tgtctttcaa tctgattttg agaagctaaa
     5821 aaactatgct ggcaggagaa atgttaattt tgcaagacta ctagaagagt tctactccga
     5881 agagttggga gtaggaaatg aatacaagag agaaacttat gcgaaattgt ctcataagtt
     5941 atcccaagcg cttaaagaag tgacagaagt taaagcggaa ctagatgcta aacttgaaga
     6001 agtgaaaaaa tatcaaacag agaggaagaa caacatgaag aatcaagtgg aatttgacga
     6061 acagaaagaa tttgaaaaag caagaaaacg ctataacaaa attatgaatg aacttagcta
     6121 tgaacactta acagttcatg aataattgag tgagggtaca gaaaaatata cgatcgcaga
     6181 tatggttaat gaagtggaaa ctcttttagc ttctttcttt gaaaatggtc acgtaaacga
     6241 tgaatggcga aaagaagaac ctaaaagggt taacgcttat attgctcgat taaaaaggtt
     6301 tattgataaa tataaggaga atagtgacaa attttctaaa gataaataag gagagagtgt
     6361 gattacattt gccagaaatc gaaggaatat tttcgccaaa aataggtaaa aaacagttag
     6421 atttcagcag ttttccgagc gtaaatagag attataatct aggtgatgag gtacagatac
     6481 taaatattga tttcaaagaa ctagacttcg ctgttgctgt ttccaacaaa cccaaggcat
     6541 tggaacgagt gaaaaaaata taccaagaga atgatcaaaa tatcaaaaat atgatcaaaa
     6601 agtatagacg agataattca aaagttgaag acgttttatt caacggtacg gcaaaacatg
     6661 atttgaatgt ttatatggca ttaggagtgg ctttgtactg taattcaatt aatcagacag
     6721 atattttggt taatatcgtg aggaatagct atcaaaaaat attaaaaata agtagcgaaa
     6781 caaaccctta ttatgcaatg aaatatcttg gggataattt attaaatgat aaaaaattga
     6841 tgcacaacac tgaagagctg ttatctaatt tggctgcgat taactttcta ttcaaaacag
     6901 gttctgaaga ttattttata gataacgtac taaaaggtgc ttacggaaca gcattttatc
     6961 cgctttacaa tatgaaacag cataaagccg aacaaaagga taacaaactt gaaattaaaa
     7021 agatgaaaga actattaggt ataggatttg taaaacccaa ttcaaaagtc actattgctt
     7081 cgttagcaac tactaacaat gatgggaaaa tagtaaatgg gtttgacgat tcagatatag
     7141 atatattgtc aaaatcatat ttttctttgt atggagattt tgcagatttg atgggtattg
     7201 atagttactt agtcaataat tttgagatca agggtagcga gctgaatgat attgtgtatt
     7261 tcgttttaat ctctctatat gcaattgatc agaataccga gtaccgacaa attgactatt
     7321 tgctagtttt ttatagtttg ttatatcagt tcgttttaac tcggttgttt aaaaatgcta
     7381 aggaagaatt gatcctgaat tctgattatg aaatagcaaa acaaattgca agtattagaa
     7441 aagaaaacaa atctgaagta atcaaattaa gaaaagaatt aagtattgcg agtaaagaaa
     7501 acggtcggtt agaatcttta gtcaaatcgg caaaaaaaga agaggaactt ttgaagcaag
     7561 agattaaaga attaaaagga atcagaaaag aacttgaaca gctgaggttt gaaaataaag
     7621 tattaaagca attgcaggag caagaacata gacaatcatt aaatggtaaa actgaagagc
     7681 cttcactgtc tgaaatgata gcttatctaa aacataaaaa cattctttta gtgggaggac
     7741 atgtaaattt tcaaaataaa ttaactgaac tattaccaaa tattaaaatg tttgatgtaa
     7801 gcgaaaataa tggaaagata cttaaaaatt tgaatacgtg tgatgcgatt tttctatata
     7861 cagattactt gaatcatggt ctatattatc gtgttatgag tgaaatttct aataaagata
     7921 agatatttta tttggataat catgtgaata tgaacttaac aattaaacgt atttacgatc
     7981 ttttatcact tatataaaga gagaagtgat aaataggaca caaaatttgg aaagtaggta
     8041 agtcactatg agagaactaa ctttatcttc taaggaatta gaaaagaaaa ctgctttatt
     8101 aaacgaaata tttagtgctg attgtataat ccaaagttta aaagaaagtt cattttcaaa
     8161 actaacagat caggaaaaaa tttcttatat gctaagttta gataatctag gaactaaata
     8221 tgatgaatca gcaagtagcg ataagctttt gattgaatta gtatcaagtc ttagtagttt
     8281 tggaaaaatg aaaagattta atgtgatatt agaaattttt gcttggttaa tcccttcatt
     8341 tatttggttt ttttatattt tcatagttga atctgagtta atagccaagg tctctcttgg
     8401 actgttaatt ccgatataaa taggttcagc aatttacttt aataacttgt atacacgtat
     8461 tcaaataaaa aagcagttat tacatgaagg attaagtggt aatatgttaa aaagaattgc
     8521 atgggataaa gataataaaa cagatattga gcttaaagaa tataaataca attatttctc
     8581 gagatgtata gatagaaagt tactgtttaa aaaagaagga ttagaattta gttttgatga
     8641 agatgaattt gaaaaattag tgatggactg gcgtaagaat ttactggata cggtacaatt
     8701 ctaaaagcaa ttgaggtcga aattccgata cttgaatcat gatctatatt atcgtgttat
     8761 gagtgaaatt tctaataaag ataagatatt tcatttggac agtcatgtga atatgagctt
     8821 aacaattaaa cgtatttatg atcttttata tgtttataaa cattaatgag gtgctaaaat
     8881 tgacaacaat aactatgaca aaaaaaatag aagtatatgt tgatggatca caagaagaaa
     8941 gggattcctt ttttgatttt ttttggaaaa atagtagaga atatgtaaaa gcgttgaatc
     9001 tggtatataa acagctaatg tttgaagagt tgataaaaga tgcaattaaa gaaatggatc
     9061 aaggttatgc tgagagagaa gaaagtattc aaaatcggat tgaaaagtta gcatatactg
     9121 atccaataga tcaaaaaaaa cttgataaag caaaagctga attatctaaa ttgagaaatg
     9181 aaaaaaataa agaagcgaga gaaatattaa cgcaagcgat agggttgaag agtcaaacga
     9241 gaacacgaga tgtagcaaaa aaattagact ttgactttgg agattttgtt gattccgcaa
     9301 atatgaaagc tagtaaagat tttggcaatg attttatcgg aattgtgacc ggacaaagaa
     9361 cgcctagaat atataaaaat agaaacaaat atggattact accttttaga ggtcgagata
     9421 ttaaaatcca taaagatgat aagttttata tttatttacc taaaggcttt aggtttaaag
     9481 tgaatttagg tagcaagcca agaaaagcaa gagatgttgc ttcagtttta caacacatta
     9541 tggatggcga ttacaaatta aatcaaagta atcttatttt tgatggaaga aaattatttt
     9601 ttaatctaac aatttctttt gaaaaaagct ataatgaaat ggaattcaag aaaggtaatg
     9661 aattgatcgt aaactttgca gatttgtgta gcggtgaatg tttaattaat gataaatctg
     9721 taatgtcttt tggtgatagc caatatataa gaaacttcaa aagtaaaatt ttagcaatga
     9781 aatcaaaaga acaaagccga ggagtctttt ctaagggcgg tcatggtcga aaaagaaaat
     9841 tagatcaaga tcgatgggaa gcaataaaga aaagagaaag taattttgtt aaaacttata
     9901 ataatcaatg ttctgcaaaa atcgttaaaa ctgctattcg caataaagct caaattattc
     9961 gtatcattga accagataag gcttatggcg aatgggctta ctatcaattg aaagaacaac
    10021 tagtaaacaa aggaaaaaga tatgggatag aagtcagaag ttagtaaatt aaacaatcgt
    10081 tgatttggca acgaaatagt gagggaaaat ccactcactg aagggtaaac tccgtcaagt
    10141 tgtgtgacca gacggaagga cgaatgttgt tgaggtttta gaaccatata aaacagcatg
    10201 tttctcaaat ttttaggata agggaaattg ctagcagttt gttttagaac catataaaac
    10261 agcatgtttc tcaaatgagt actttaaatt aattgatggc gttcctgctt tagaaccata
    10321 taaaacaaca tggatttaaa acaagaagta agtcagttct ccttttactt tagtaaggaa
    10381 aaataaagta ttctcaataa aacttaaaat aagcaaacct ttgttttaat agctaatcga
    10441 agtaaaaccc aaagccatat gaacccctga atcagcgaaa gtttatgatg atttggatta
    10501 ctaatagttt aggaattaga tggtaaatct aaattaaaca atcgttgatt tggcaacgaa
    10561 atagtgaggg aaaatccact cactgaaggg taaactccgt caagttatgt gaccagacgg
    10621 aaggacgaat gttgttgagg ttttagaacc atataaaaca gcatagttct caaattgttt
    10681 aaaatgattg tcaagttaaa aaaatctggg atcatataaa atagtatagt tctcaaatat
    10741 gattagaaac cctattaaag agcaatatta agccaactta cttaaatcgt ttaattaaga
    10801 acaataatct cttttagtca gacttttagt ctgacttttt tgtatttcca aaatttattc
    10861 tttacaaaaa cgaacatacg tttgtataat tgcttgtgaa tgggggaata aaaatgtttg
    10921 attacagtag agaaccggtg aatgactatt ttttaatcga catgaagtct ttctatgcta
    10981 gcgtggaatg tattgaacga aatttagacc caatgactac accgttaatt gtcatgagta
    11041 gatcggacaa cactggttca ggtctgactc tagcttcttc acctactgca aagaaaaaat
    11101 atcatattac taatgttagt agagctactg atttacctta tccattacca aaagaacttc
    11161 atattgttcc accacgaatg aacttatata taaaaaaaat atggaagtta atcagatttt
    11221 tagaaagtat gtcgcagatg aagatttatt gatttattcg attgatgaat caattttaaa
    11281 agtgacaaaa tccttaaatt tatttactag ttcaggaaca agaagcgaga gaagaagaga
    11341 attagctaaa ataatcaaag atgagattca gaaagaaaca ggtctaatag caacagtcgg
    11401 aataggagat aacccattat tagctaaact tgcactggac aatgaagcga agagtagtaa
    11461 agaatttata gctgagtgga cttatgagac tgtacctgaa aaaatatgga aaataaacga
    11521 gatgactgac ttttggggca ttggatcacg catgaaaaaa agattgaatc atatgggaat
    11581 tatgagtata gaagatttag caaattggaa tccctacaca attaaaagtc agttaggtgt
    11641 aatcggactc cagttatatt tccataccaa tggaattgat cgtacggata ttgctattcc
    11701 accagaacca accaaagaaa agtcttatgg gaattcacaa gttctaccaa aagattatac
    11761 gaggagagaa gaagtagaac tagtagtcaa ggagatggca gagcaagtag ccattcgaat
    11821 tagacaacac aattgcaaaa ctggttgtgt acgcttagca attggtacgt caattttgga
    11881 agaaaaatca ggtttctcgc accagatgaa aataccaatt acagataata cgaaagaatt
    11941 acaaaattat tgtctttttt tattcaataa gttttatgag gggcaggaag tacgacatat
    12001 aggagtaact tatagcagat tagtatacac agattcccta caattagatt tatttagtga
    12061 tccaagaaaa aagattgctg aagaaaattt ggataggatt attgataaga ttagacaaaa
    12121 atatggattt acagctatta tacatgcttc atctatgtta gaaggtgcaa gaagtattac
    12181 gagaagcact ttaataggag gacacgcagg aggattagga ggtttgcata atgattgaat
    12241 ataacgatag aggacgttta aaatggtcgg gattttattt gtctgatcat actgaaagaa
    12301 tcaatgcaga caatgtacaa cgtgctaatc ataatttggc aaaagaacaa atgacgactg
    12361 aagagattgc gaaaatactg agaactgctg tcataaaaaa tagacccgtc tgcattcaaa
    12421 aagaggaacg aaacgctgaa ggttactatc cagatgatgt agtaggaaaa attattggat
    12481 atgacgagct tggtgtattt gtggaagaag aaaaagttca ctatgatgaa attaggaatg
    12541 tttctttctc ggaattatta aaatggagtc agatatgaac attattaaaa aatacgaatt
    12601 cggttatatt acgtatgaac agctaataga ggaattatgg ggatatggtc aacaattaat
    12661 taatgaagtg gggattgatt gcttttgttt ctatattgaa tccggagcag gataccataa
    12721 gaacagatac tatatatttc catatccacc aaaatagtct cttagatatt tttaaatttt
    12781 ctccaaatac tttcaattat tgtgaaatat ttccctttaa ttaatcaaat tttttataaa
    12841 agtaatatat aaaataaatt gcttgaagta ttatatttag atatcataat ttatggaaga
    12901 aaaattaatt aaaaaggatg agattataat ggggttactg gttccgagag ctaatactta
    12961 tactatacct ttaacgaaac gccaattaat tgctaaaaga tatcaaagga ttactagagc
    13021 catcaataga gagttttgga attctgaaag tgatactgct cacagtttat acgtaggttc
    13081 ttatgggaga ggaacggcaa ttagtactag tgatatagat ataatagttg aattaccgat
    13141 ggcagaattt gacagattca aaaattattt atcaaatggc ccgtctaagt tattacaagt
    13201 tataaaaaac gcatttcagg aaatactacc aaatagtgat attagagctg atggacaagt
    13261 tgttaaaata aatttccatg atggaatcaa atttgaaata gttccagctt ttaatgaaaa
    13321 agattattgg ggtgagtcaa aagggtttat ttatccagat tcaaacatgg gagggaactg
    13381 gaaagctaca aatcccaaaa aagagcaaga agccatgaaa ttaaagaata caaaaagtaa
    13441 taatttatta tatgctacgt gtaaacattt tcgacatgta cgagatacag aatttacgag
    13501 ttatcatctt tcaggaatag taattgacag tttcgtatat gaggctatgg gaaattggaa
    13561 attcgttgaa aataacagtg ggggtcaaaa cattagttct gtatcttatg agacagcttt
    13621 attagaatac tataactcac ataaagttat gggcggttta aatttatact ctcctggaag
    13681 caatcaattt gttaattctg atagtagtat catatgttta gaaaaagtat taaaaaaaat
    13741 agctttataa agttttatgg aggaacaaca tgggagacga aaaaaagcgc ttggaagttc
    13801 aattacgtga agcatatgga cgagtaacgt atacttacac cactcattta aagcaagccg
    13861 ataatattat ttctgacaat aaaattttaa ggtgtcttca aattggtttg tcagccctta
    13921 ctactggagg ggtacttagt accattatta ctgataatag tgagttaacc tttgttgctg
    13981 gattatgttc aacaatttct cttggattaa acttatactt taagaatttt gatcttgctg
    14041 aagaagctaa aaatcatcaa attgttgccg ataaattatg gttgatcaga gaagaatacg
    14101 taagcttact aacagattta aatcgtttaa gtgttgaaga tattgtgaaa aaaagagatc
    14161 aattaatgaa cgaaactcat gaagcatata aaaatgcccc taaaacaaat agtaaaagct
    14221 atgaatcagc tcagaaagca ttaaaatctg aagaagagca attttttaca gaagaggaat
    14281 tggataagat gttaccagag catcttagaa aaaagagttc taaatattga actctttttt
    14341 tatgcttaaa attctcttgt aatttttcta ttcatctata tttaagaaga aatatgatga
    14401 tagaaacatc tcctaaagcc accgcaagcc gttctaagca tagcttagcc cgacattgct
    14461 agatatgctc atctcaccat gaacgtttcc taaagccacc gcaagccgtc ctagccattg
    14521 cctagtccga catcgctaga ttagtgcatc tcgccataaa agttttctaa agccaccgca
    14581 agccgttcta agcatagctt agcccgacat tgctagatat gctcatctca ccatgaacgt
    14641 ttcctaaaac caccgcaaac cgtcctagcc atcgcctagt gcctagtccg attttgcttg
    14701 atcggttcca tttctgttta tttagtgggt caacaccgta atcttaaata gccatcgcct
    14761 atttaagatt actaggtctg ctttgaaggt aaaaagtctt tcattcttca agacttttag
    14821 gctttcaagc tacgcctgaa agcattgaaa ttcatcgctc tttatttggt tttgccattt
    14881 ataattcctt acgcccatta tctttttcgc ctgtcttgcc ttgtcagaag tgtttagagc
    14941 ttatctcatc tttggattaa tacaagtggc gtaaattttt ttctaaacgc aagcgttacg
    15001 aaaataattt acttaaacac ttgtattaat ccggcagata agaacgcatt ctaatcactc
    15061 ccgacaaggc aagacaggcg aaaaagataa ataaatgaac gaaaggaaga aacataaaat
    15121 gcaaaaccaa atagcgatga agaaaaaacc acctccctct aaaacaatcc aaacaaaaaa
    15181 gaattttgaa aaaccaaaaa tcaaaaacag aaagaagcgt tcaacatgag agatagaatt
    15241 ttagaagtta atcaagaaat ggtaaacaag ttaacaaata tcagtgggaa ctatgaaaag
    15301 tacgatggta ctttacttga taattatatt tttcacgata cacaaaatat cagaattggc
    15361 agaagtaaac cgagagcgtt cattctcatc aaagagaaat atttgaatga atggaatagc
    15421 acctatgaaa tggttatgac tgataacttt gaaaaggtat tggatttcag aaatgctttg
    15481 ttgggttctg atatgggaaa tagcagtctt gaatctattg aagttgaaaa caagaccgag
    15541 aatgaaggtg aaggattgat taagaatgtc ctgaaaatcg aacaaaaata tatcagtgaa
    15601 gatagaagtt tttatagagt cacaagctcg gttggtctag caaactttgc tactaactta
    15661 attggaaaca atgctagtga atctttgcta gtgatgtgtc tgaatacaaa gaatgaggtc
    15721 attgcttata gcgaagtttt caaaggtgga attaatcaat cagtagcgat accaagagag
    15781 atttttcaac tagctttgct aaacaattca gcaaaaatcg cagtatcaca taatcatccg
    15841 agcggatatg aatttccaag tgaaaatgac aaaagtttta ctacgaattt gaaaaacgca
    15901 ggaaaaacaa ttggtattga attgttagac catatcatcg taacaagtaa aaacacaagc
    15961 tattactcat ttcaagaaaa tggttcttta taaaaattag agaaaagagg aaaacaagat
    16021 gggcgaaaga acacaagcgt taattacagt caaatatggt aacagcaaaa cgaaagtaag
    16081 cgtgtcaatt cattatcagt ggggatatgg tcgattcatg ttgatggacg ttttgaatca
    16141 aggaatatat ttaatcaata aagcatatga cttacgagat gaatcaagag aagaattagc
    16201 aaaaggaatc tttgatagaa gtacaggagt tatcaatttt gttacctatg aatctggagt
    16261 gagtgcttat gatgaaacag ggtttggtgg gcgctatgaa ccttatcaag aaacgattga
    16321 agatgtgatg aagttttcag ataacaatga tggctatgct catttagaga tggtgttcga
    16381 tgaaaatgga tttagtctag atgataaaac cagattgcga ttgtttgatt ttgagggtca
    16441 acagatagga ctaaacaagt tttgtgaaag cggaaaagag tttgctacga aagattttat
    16501 taagggatac agattattgc tagaaaatta tggaataaac ctagaagaag agagcgtgaa
    16561 ataagtgttt gaaaaaagaa aagaccagtt ccctaattta gaagcgaaaa gtcaaaaagt
    16621 gattgatggt gaaatgattg aattattcca aaaaagttat ggaaccatag aaactaccgc
    16681 aagttgcaaa tactactttc aaggaaagag aaacacgttt aaaggctttg ccgattctga
    16741 tgaagaattg gcaaagaaaa tcattgattg gcttgtcagc agtgaacacg tagcaagaga
    16801 agataccgtt agaacaacga ttgagggtga agtcctaaca ggtactagat gtgtttgttg
    16861 taatcatgaa tttgtagaag atgaaggttg ctaccgaatc aatgaatatg gtgattttat
    16921 ttgccagagt tgccacgaga tagaatttcc aaccgaaaga agttggtttg aatacatgct
    16981 acaagattat gagatggaaa cagaaaatgg tgaagtagtt ttttgtacca aagaggaatt
    17041 gagtaagaga agcgatgatg aaattgaaga ttggtgtgtg tggttagtag ggacagatga
    17101 ttgcgttgca tattacacaa cagcagaata ggagtgtgaa tcagcatgga aggaatggat
    17161 tttatagatc atgaagattt gattgatttt ggttatacat ggaaaggaat ggtaggtatt
    17221 agcagaagtc ttgctaatgc cttttacgaa agaaattatg cagtctatgt cctttatgat
    17281 gacaatacag aaagccttgt tgatgaagag tataaattgg atttagagaa tgttttatat
    17341 gggatagaaa aagaagattt agcaaaatat attttttcat ggttgggtca ataaacgaaa
    17401 gagggtagca acatgacaaa attcaaaagt aaaggtatgc aatggtgttc ttattattat
    17461 gttgggaatg atgaagagct tttgagaaaa gccaaacaaa aaggttatcc agataagatg
    17521 ttaaaacaga ttggctatta catgccagaa gagagaatag aaaaagaatc ttgtaaggtt
    17581 ctttttgata gtgaacagct attagttttg ttaactcaaa ataaattgat ttgtcaattt
    17641 gaaaataatt aatatggggt gaagaaccat gaatgagaat aagatggtat taagtgaata
    17701 cgcaagattg agaaccatta attggttgaa tggtatgcaa agtagtggcg cttttgatgg
    17761 atttgatgag gagaatatgg actttgaaag tttatcgaat gaggatatta gatattattt
    17821 tatcgaatgg tattttacag aaccagatga agaggtggaa gatgaaccag aattaatttt
    17881 agaacgtcaa agaattttat tcagtgaaca agatagtaag aggttgtatg gtttatatga
    17941 cttacaactg aaaggattaa aagcgcagta tgttgctgat agttacgatg atatggtaga
    18001 acagggggca gatagggttg tcacttattc agaagatgat gaagaggaat gggaaaacta
    18061 ttcagatgat gagatatgct ttaatgatgg gtatatgcct attgaaatca ccgaagaaga
    18121 atatgataaa gtgtcggata gcgatgagat tggtatgtgt acggttgtaa gtggattgga
    18181 tatagatgtc caaagagtag aggttgcata ccagaagatg aaagaacgta taaaaagtaa
    18241 agtagaatct tgatttgatt ggattgtttg caaagtagct aattaagagt ataatataat
    18301 cataaatgta gttaatggat ataataatga gtatattata ctcaaaagag gtgaacgaaa
    18361 tgaaggtaaa aaatccaaaa gaaaaaagca gaattcaagt cggaatagac aaggatttaa
    18421 aagaaaacgc agaaatgata ttggaagagc tagggttaaa tccaacaaca gcaatcacaa
    18481 tcctttataa gcaagtggtc gctagaggtg agtttccagt agagataaag ctaagcgaag
    18541 aggaaaagca aggaatcaga ttgcaacagc taactatgga tatgccagtt gatgttctag
    18601 atacagatga aaagttagaa gagtggttca atgaagccta atgagatagt aacggtctat
    18661 gttgcattta cagataaaaa tggtggtaaa agaagaccta ttttggtggt tagtgataaa
    18721 gaagatagag tagaattttt tggaatcaca agtcaatatg agaataaatc agaccaaata
    18781 aagagagtgt attttcctat tgcagaatgg gaaaaagtag gtctaaagaa gcaatcatgg
    18841 attgacgtag ggtctttaaa ggctattccc aaaagccgag agaacatatc atttaaaaag
    18901 gttggtatgc tatcagctat tgatacgaaa aagctaaata ggtttatcga gagattacaa
    18961 agaaaagtct aacaaggctt ttcttttttt gtttatatgt atattaagat atacgtttat
    19021 ataaacgtat ttatgtatat taaaatacac gtaaatatat acgtgattat aaaaattaag
    19081 aaaatattaa gtattgttaa atagtgataa ttagtaatat acgaattata agatacattt
    19141 tatatttaat gtatgtaaat aaataaaatg actgacggat attgaatagt aataaataac
    19201 aataattcag acggatacag tgagtgaagt agcttgctac gaacgaacag gcagaatgaa
    19261 acggtagttt cattagagca agtcgcttgc gacgtagcgt taccactgat gtaactggca
    19321 agttacatag agagagtttg cttgcaaacg aacgttaccc acttgaatag aaaacaaaaa
    19381 taacatatta gtgaattttg aataggagcc acaactcaac caaccgcttg cggtgggtga
    19441 gtcccacgga ggaaaagacg aacgaacggt cgcgt
//
LOCUS       DACDYJ010000048        19431 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDYJ010000048 DACDYJ010000000
VERSION     DACDYJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.048"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..608
                     /locus_tag="IWM51_001832"
     CDS             <1..608
                     /locus_tag="IWM51_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971253.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
     gene            628..1656
                     /locus_tag="IWM51_001833"
     CDS             628..1656
                     /locus_tag="IWM51_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9971254.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1653..2411
                     /locus_tag="IWM51_001834"
     CDS             1653..2411
                     /locus_tag="IWM51_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9971255.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2497..3237)
                     /locus_tag="IWM51_001835"
     CDS             complement(2497..3237)
                     /locus_tag="IWM51_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9971256.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3298..4551
                     /locus_tag="IWM51_001836"
     CDS             3298..4551
                     /locus_tag="IWM51_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9971257.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4852..5100
                     /locus_tag="IWM51_001837"
                     /pseudo
     CDS             4852..5100
                     /locus_tag="IWM51_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5222..5548
                     /locus_tag="IWM51_001838"
     CDS             5222..5548
                     /locus_tag="IWM51_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9971258.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5654..6328
                     /locus_tag="IWM51_001839"
     CDS             5654..6328
                     /locus_tag="IWM51_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9971259.1"
                     /translation="MLLFLLLLVGLALIFNEQIKDYLVRETGDKYAIANVTKEDLKKN
                     NDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINLPIFKGLDNTSLLY
                     GAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLTDLENVYTYKTFFK
                     EKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTPIKDATDDMRSAFN
                     LETKTF"
     gene            6638..7490
                     /locus_tag="IWM51_001840"
                     /pseudo
     CDS             6638..7490
                     /locus_tag="IWM51_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            7611..9476
                     /locus_tag="IWM51_001841"
                     /pseudo
     CDS             7611..9476
                     /locus_tag="IWM51_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            9490..10932
                     /locus_tag="IWM51_001842"
     CDS             9490..10932
                     /locus_tag="IWM51_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9971260.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            11040..11741
                     /locus_tag="IWM51_001843"
     CDS             11040..11741
                     /locus_tag="IWM51_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9971261.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11900..12379
                     /locus_tag="IWM51_001844"
                     /pseudo
     CDS             11900..12379
                     /locus_tag="IWM51_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            13226..13801
                     /locus_tag="IWM51_001845"
     CDS             13226..13801
                     /locus_tag="IWM51_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP9971262.1"
                     /translation="MKFTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13801..14241
                     /locus_tag="IWM51_001846"
     CDS             13801..14241
                     /locus_tag="IWM51_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9971263.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14257..14958
                     /locus_tag="IWM51_001847"
     CDS             14257..14958
                     /locus_tag="IWM51_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9971264.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIHQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(15446..15871)
                     /locus_tag="IWM51_001848"
     CDS             complement(15446..15871)
                     /locus_tag="IWM51_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971265.1"
                     /translation="MKKLIVLFILSAGLLTLTGCNLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQETDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATITFTKIGNYRLKDEDGNIYSL"
     gene            16245..17216
                     /locus_tag="IWM51_001849"
     CDS             16245..17216
                     /locus_tag="IWM51_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9971266.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKELLEKGVTAIFVSNYNMSIGAIECFNQEGIRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGEQAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            17248..18645
                     /locus_tag="IWM51_001850"
     CDS             17248..18645
                     /locus_tag="IWM51_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9971267.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHPEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            18804..19427
                     /locus_tag="IWM51_001851"
     CDS             18804..19427
                     /locus_tag="IWM51_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971268.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         6632 a         3186 c         4057 g         5556 t
ORIGIN      
        1 cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac caagcaggag
       61 aagctgctgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt gggaatcaca
      121 atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct gctacgaaga
      181 ttgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgattgt ggtcagatca
      241 aagcaactgg gttgacgcaa gaagcatttg gccaaatgga tatacaggaa aaattatgga
      301 tttatgatca gcgagaaaca aaaagtccat ggatcttaac gataaaatta tctcagcctt
      361 tcacaaccga tgcaggattt tctatgcagg atgtcctata tttcaagaaa gctgaggagt
      421 atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca acgaatgtgc
      481 aaacagatta ttcagaatat ttaaataatg gtcgggcgtt taaacttgtc ttaaccaaag
      541 aaaatcagat agctggtaat tactcagcag agatgacatg gacattagca gatgcgccga
      601 cagaataaag atcaaggaga atgattgatg aatcgaatat ggaataaaag tcatcgaaac
      661 aactgctctt tacttctgat ccttctcctt ggattcatcc tttggcagac acctgtccat
      721 gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat tgataagaac
      781 aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc ctacacggtc
      841 tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc tacgacgaat
      901 tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac attaagttat
      961 gatatgaaaa acttagcaaa aacagtggga gaaataactg tccctcctca aagtgcggtc
     1021 actgagacac tttcactgaa agctccagaa aaagagctgg ctggtgtggt cgcaggagga
     1081 atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat
     1141 cagttttctc aagtgaaagc aatcttgatt cgaaatagcg aaaaagagtc ttccccaaat
     1201 ttgaaattaa aaaaaactta tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat
     1261 cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga aatctacaaa
     1321 aaagaaacaa aagagcctta tctttcaata aaaaaagaaa aaatagatgt agcacccaat
     1381 acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg acattatcaa
     1441 gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga ttttgaaata
     1501 actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa tcagccagaa
     1561 acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt atttgctgga
     1621 ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg
     1681 ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat aagagctcag
     1741 ccactacaga aggaacgatt tctgtaacaa aaccagatgc cgcaaatcct gaagacgttg
     1801 ttccagttcc tcctgttgat ccaaatgatc ccgatacgcc gtttacacct aacgaaccaa
     1861 atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat tttggaacaa
     1921 tcaagttagg tacagcaaaa acagtatatg ctgcattaca aaacggaacg gacagtacag
     1981 ggacagcaac acaagtacag aatttcgtac aagtaacgga taaaagcgga atttatgccg
     2041 gttggaaact atcagtcaaa cgaacagaat ttattggtac aacatctgat acgaataaac
     2101 taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca actaatagca
     2161 gtggaacgcc atctactatt tctggtactg gcgctgaagg aattgagatc ccagcagatc
     2221 aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg atttattcat
     2281 taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct gtatccaatt
     2341 atgccgtaga caactacgtt tcaacattca catggtcgtt aacagatgct cctacagaag
     2401 ggaaagcgta aaatcaaatc aatgacttca gggagtggag ccgattcaag gccaactccc
     2461 tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag ttttttggaa
     2521 gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta aggagtcacc
     2581 attgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg ataagttgat
     2641 acagtaagat tttaatagta aaaagtaagc atacttttcc aactctgtct tcaattgttt
     2701 taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt ggtttttcaa
     2761 acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac ttccaccttt
     2821 ttgatcaata aagattttat cgacatttac gatttgctgt tctttccaat tgatctgttc
     2881 tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc caatcggaag
     2941 gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt
     3001 actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga gtgattgctt
     3061 caagtcgata tcgataaaga aaacatcgtg attatcaata gtacccaact ggatcaactg
     3121 attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat tgaccaattc
     3181 tttgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact ttttcatatg
     3241 caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag gaataagatg
     3301 gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg tatctattta
     3361 ttcttttcga aacaactgct agtaaaaaac agctttggtt ttatagcttt gatggtggtg
     3421 acacttttat ttcctatccc taaagtgagc tttccttacc tagttggctt attattgatg
     3481 tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag taatcttttt
     3541 tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta tttaggaaag
     3601 agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt ctatctatta
     3661 gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta tccaactgaa
     3721 cactattttg gaggtatttg tattttatta agctggggcg tgatttactt ggtattggga
     3781 agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat agtcatcagt
     3841 tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga aagacaacat
     3901 caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct agaggaattc
     3961 aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga aaataaaaaa
     4021 gaaacgttgg aatttttaga aatcttagaa gatcattctg cagctatttt tgaaaaaccg
     4081 caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt tttaaaattt
     4141 ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa
     4201 acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat ttctaacgca
     4261 ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga agaaaaagag
     4321 ggattacgtt ttagtatagc gaacaaggca aatccgacga atttgacgct atcggagatg
     4381 gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa
     4441 atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat
     4501 ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc
     4561 aaaggataag cttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa
     4621 agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca
     4681 aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt
     4741 agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc
     4801 agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagaa aatccctacg
     4861 atcacacaaa aaatgctgac ccagcagtta agagagttag aacaagatgg cattgtccat
     4921 cgaaaagtct ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa
     4981 tcattaagag agattctagt agcgatgtct gtttggggag aagaacgtat cgaaaaacaa
     5041 aaagcctcag gggaagaagt cacattactg catacacatg gtggctacct aaattactga
     5101 tttctatgac atttatttta aataaaagaa aacaggagtt tttttaaaag catcatctat
     5161 tttttatgta aaaaaatggt atgattaatc aagtaagaaa tgattggaag aaggcaagca
     5221 aatgaatagc atggaaaaag ttaaccagtt tagagatgaa cgtaattggc gacaatttca
     5281 taacgaaaaa gatttggcta tttctatttc tttggaagca tctgaattgt tagaactttt
     5341 tcaatggaaa acgccggaag aagtaaaaga aacacaatta gaacgaatca aagaagaact
     5401 ggcggatgta ttgatctatt catacatgat ggcagacaac cttcagctgg atctagatga
     5461 gatcattgaa gaaaaattga tcaaaaataa tttgaaatat cctgtagaaa aaagcgcagg
     5521 tgtacgaaaa aaatacacag agttttagaa atgtgtcatt cggctgttgg aaagagcaaa
     5581 agaaaaacag taaaatgagt ctggagggag agaaaatgaa aaaaaaggaa aagcaaaaag
     5641 atggctagtc aatatcctct tgttcctctt gttattagta ggactagctt tgattttcaa
     5701 tgaacagatc aaggattatc ttgtcagaga gacaggagat aaatatgcta tagcgaatgt
     5761 aacaaaagaa gatctgaaaa agaataacga taaagatgtc agttttgact ttgatgcggt
     5821 agaaccaatg acgacagaag gggtcatgag gtcgcagatg agaggaacag atctaccagt
     5881 gattgctagt atcgcagtcc cttcagtttc aatcaattta ccgattttca aaggcttgga
     5941 taatacgtca ctactttacg gagcaggaac attatctcca gatcaagaaa tgggaaaagg
     6001 aaattatgcc ttagcaagcc atcgtgcaac gaaccctgaa ttgttgttta caccgctgga
     6061 aaatttagag atgggtgcca agatctattt gacagattta gaaaatgtgt atacatacaa
     6121 aacatttttt aaagaaaaag ttgctcctac agatacccaa ttactgaatg aagtcgagga
     6181 aaaagaaatt gtcacactaa ttacttgtgg cgacatggac gccgtcacgc gactagttgt
     6241 acaaggagaa ctggaaagtg ttacaccgat aaaagatgcg acagatgata tgagatctgc
     6301 tttcaatcta gagacaaaaa cattttagga tactgggaca taagtcgtta cagattaaaa
     6361 acagccaaag gatatgaatc gataaggaaa aaccaatttg ctgaaaaaaa gaggaacgca
     6421 aagaggtgca tctctgattt ggttgttttc tagatagact tgtgttccag tttttttatt
     6481 ttttatatga aaaggcttgc ataaataaaa attcgatggt atgatgattt tgaataagaa
     6541 atggattgtt actgtacggc aggcaaaacc agaattcagc taagaaacag taggcgaatt
     6601 ctaggttttt ttatttttct tgagagggcg aagcaaaatg aaaattgata aaattttgaa
     6661 taacaatgtc gtcatttcga aaaatggatt cggggaagaa gtcgtttgta tgggaaaagg
     6721 actagctttt caaaaaaaag attggggacg agatttcgcc agaagcggtc caaaaagaat
     6781 ttattttaag agattctttt gctaaaaacc aattccagca gctgatggct gatattccgg
     6841 cagaggagat cgaatgggtc aaacaagtag tcgaactggc tgaagataaa ctaaaagttg
     6901 aattttcgcc taatatttat ctgacattga ccgatcatct tcattatgcg atcacacgag
     6961 caaaagaaaa tatccaactg cctaatccat tattatttga aacaaaaaaa ttttatccga
     7021 aagaatatca agcagcgaaa gaggctcttt tgttcgtgaa acaaaagacg ggtgtggaac
     7081 tttctgagga tgaagccggt tttattgcct ttcattttgt gaatagccaa caaggaaacg
     7141 aagacatgca agtgacgatg accgctacca caatggtaag agatatactg agtatcatca
     7201 gtaaattctt cggcatcgtg ttcgatgaag aatctttgaa ttatcagcgt atcattactc
     7261 atctgcagtt ttttgcccag cgttatctca aaggggaagg atctgacgag caagatgaat
     7321 ttctctacgc attagttcaa ggaaagtatc caaaagcgtt tcattgtgtc gaacggatca
     7381 atgagtatct tcgacaaacg caaaaacagg aaatggggat cgcagagcag atttatttaa
     7441 cgattcatat tcaaagagta gtaagtgaga aaaaaagcat ccaatcataa ggattgttac
     7501 tgtatggcag gcaaaacctg gattgaaaaa ataggttcac gaatgtttgt gactgttttt
     7561 ttcaatctgg gtttttattt tttaaataaa aaagaaagaa ggaaaaaaca atggataatc
     7621 aagcagttgg tcgtcgtgta tgggaagcgg taggaggaca aaagaatgtc aaaagtttag
     7681 tacattgtgc tacgcggttg cgattcagat taaaagatga atctttagca gatacgcaaa
     7741 agttaaaaga agacccggac gtgatccaag tcgtacaaag tggcggtcag tatcaagtag
     7801 tgattggcag taatgtagca gatgtttatc aatcgatcgt ggatgaggaa ggtcttgcag
     7861 tttcggagga tggaaaagaa gagtccaaaa atattttgaa tcgtctgatc gatattatct
     7921 ctagtatatt tacacctttt ctaggggcga tggcagccgc cggggtattg aaaggattct
     7981 tatcattggc aacggtgatg ggctggctga cagctgatag cggtacttat caaatattgt
     8041 ttgctgcagc agatggtgtc tttaccttct tacctgtcat gcttgctttt acagcagcta
     8101 aaaagttcaa agccaaccag tttttagcag tagcaattgc tatggcactt gtgtatccag
     8161 ctatcacagc agttgctggt gcgggagaag ctatcagttt ctttgggatc ccagtgattc
     8221 tttcaccatc tggttatact tcttctgtca taccaatcat tctagccgta tgggttcaaa
     8281 gtaaattaga gccattcgta aaaaaagtga ttccgcaatt tctgcaaatg attttagttc
     8341 cgcttgtcgt tttagtagtg atggttccat taacatttct tgctttagga ccaatcggga
     8401 cggttgccgg taatgcttta ggcggtctct tcaactcgat ttatggattt agtccaatcg
     8461 tagcaggtct aatcatggga agtttatggc aagtattcgt gatgttcggc atgcattggg
     8521 gattcgtacc gatcatgttt ttgaatatcg aacaatatgg gtttgatgta ttgatgccaa
     8581 tgcttcttcc agccatcttg gcacaaggtg gggctgcttt agctgtagca ttgcgaacaa
     8641 aagatacgaa acttcgaaca ttagggattt cttctacagt gacttcgtta tttggaatca
     8701 ctgaaccaac tgtgtatggt gtgacattac ctttgaagaa accattcatt gccgcttgta
     8761 tttctggagg tattggtgga gctattatcg gattttccgg agtaaaagct ttctccagca
     8821 gtttggtcag cttattgacg attccgactt tcattagtac ggtggatggt gtagagtcaa
     8881 atgtgacagt agcagtaata gctacaggaa ttgcttttgt tcttgcattc gtaggtacat
     8941 tgattttagg atttgacgaa caaacacaag acaatcaatt agaaaacaaa catgcgaatg
     9001 caggagaacc gattacttca gcacggcata ccttaaaaag tcctttaacg ggtaaagtac
     9061 tgccattgag tgaagtacca gaccaagttt tttcttctgg cgtgatggga aaaggtatcg
     9121 ctattgatcc agaagtagga gaactagttg cacctgcaga cggagagatc acgactattt
     9181 ttccaactgg acatgctgta gggatcacaa cgactgacgg agcggatatt ttgattcata
     9241 tcgggatgga tacagtagaa ttaaatggga atggctttga gatcttagtc aaataagggg
     9301 atctcgtcca agcaggagat ttattgattc gttttgacat cgaagcaatt agagcagctg
     9361 gctatagcgt aatcactcca gttgtcatta cgaatactga tgcatttgcc gatatacttg
     9421 aattagacca aaaagagatt attgcaaacg aagatgttct ggcaattgta aaataaggag
     9481 gaaatcagga tgcaatcacg tttttcagaa aaatttttat ggggtggagc aactgctgcc
     9541 aatcaattag aaggggctta tgatcgagac ggaaaagggc tatccgttgc agatgctatg
     9601 cctggaggta agcagcgctt tgcgattatc ggcagtgaag aatttgactg gacgattgat
     9661 caagaaaaat atatttaccc gaatcatcga ggaatcgatc attacgatcg tttcaaagaa
     9721 gatcttgcct tgtttgcgga aatgggattc aaatgttatc gtttttcgat tgcctggaca
     9781 cgaatttttc caaatggaga cgaaggtact cctaacgaag cgggattaga attttatgat
     9841 cagctgattg atgagtgctt aaaatatgat attgaacctg tgatcacgat ttctcattac
     9901 gaaatgcctc ttcatttggc aaaagaatac ggtggctgga aaaatcgaaa actgatcgac
     9961 ttctatgaac gatttgctca gacagtatta gagagataca gcagtaaagt caaatattgg
    10021 atgactttca atgaaatcaa ttcggccttt cactttccag ctttaagtca aggattagta
    10081 aaaagcaatg gagcaggaga gtaccaaaat attttccaag catggcataa tcaattcgtt
    10141 gcaagtagca aagctgtaaa aatcggccac gaattgcgtt cggatatcca aatcggctgc
    10201 atgattatct atgcaacaac ttacagcatc gatgccaatc cagtcaatca ggcagcaaca
    10261 atgatccaaa accaagaatt caattttttc tgcacggatg tccaagtacg tggagaatac
    10321 cctgcataca cagcgcgtac ccataaaaaa tatggagtcg atccggaaaa attagagcaa
    10381 acggaaaaag acttccagtt gctaaaagaa tatccagtgg actacatcgg atttagttat
    10441 tatatgtcta cggcaatcaa tgagactgat ccacaagcag caacttcaga agggaacttg
    10501 ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat ggggctggca gatcgatcca
    10561 gaagggctga gaatcgcatt gaatgaatta tacaatcgtt accagaaacc attattcatt
    10621 gttgaaaacg gtcttggagc cattgatcat atggaagagg atggcacgat tattgatgat
    10681 tatcggatcg attatttacg tcgtcatatc gaagcgatgg ctaatgcagt agcagacggt
    10741 gtagatttga tgggctatac gccttgggga tgtatggatc ttgtcagtgc atcaacagga
    10801 gaaatgagta aacgttacgg ctttatctat gtggatctag acgatgaagg aaatgggaca
    10861 ctgaatcgat caaagaaaaa atcttttgat tggtataaag aagtaattgc ctcaaatggc
    10921 gtaaacttgt aattaaagca gctgaatgaa aaagaacaca cttctcacat gtgatttgtg
    10981 ttcttttttt attttttatg tgctattctt ttagtaatag taaagcaaag ggtgacgtta
    11041 tgaaaaggac acgtgtacta tacttggaag tcgcagataa aattaaagaa gatattttct
    11101 caggaaaata tccagtcggt tcgatgttac cgacagaaag tgaattagaa gaactgtttc
    11161 aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct ggcatcagat gaatatgtcg
    11221 caaagaaaag tggtcgtggg acgacggttc taagcaatcg tccttacaat cgattatcca
    11281 aagcggcaag ctttacgcaa atcttggaga actcccgata tgaagtccgg aaagaaacgc
    11341 tggatttgca attggtccat ataaagaagg aagatccttt gttttccttt ttcggtgagc
    11401 aagctgtttg ttttaagcgc ttatattatt tgaacaatca gccttatatt tattttgttc
    11461 attatcttcc acaagaaatg atgcaattca gcagagaaga atttggcaaa gaatctttgt
    11521 atcgtttgct gacgaaaaaa ggatatacta ttgactcatt ttctgatgat ttctcggcag
    11581 tatttttaac agaagaagaa caaggaattc tgaaaacaga cgcacagatc ggcatcaaac
    11641 ggatacggaa atcgtctacg caaattgggg aaatgattga atattcggag gcggtctacc
    11701 atacggagct gcatccttac catattgatt atgaaacata aataaaaaaa gaggttgtga
    11761 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagcttc tcatattttt
    11821 cgtttggtta ggcttattac cgcagataca cggctgtttc tggattacca tttattctgg
    11881 ataagggggt gtggcacgat tttcagaagg aatctatgaa ccaatcactg tattgatttt
    11941 agcttcgatg gtaaccccat tcggcttgac gatcgcttat ttcttaggga aaatcattcg
    12001 taaaaatata ttcaatcgtc aagaaatcga tacattgaag accgcttttc ctatggggat
    12061 ctgtcagatc acagagggat gtttccctat cgtattgaac gacttggcac gcaatgtcat
    12121 agcaacggga gtcggtgggg cagtaggtgg cggattaagc atgttttggg gcgcagatag
    12181 ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg accagaccat gggcattcat
    12241 tggtgcattg ttagctggtt cttttgtcac aggaataacc attttattat tgaaaaaacc
    12301 agtagatccg aatgcagaaa tcatccaaga agaaaaagag gaagaagaca tatcttggga
    12361 tgatttgaca attagttagg agaggcagaa tggattacaa gaaaaaagta caatagatgt
    12421 accttgaggc aggaatggta tgaaagaatc agagttagaa agaatcgaat atacagattt
    12481 tggtttagga gcgatcgaaa ctgtccagag catcggcacc ctccttgaaa tgacgatgaa
    12541 ggaaaaatga gaacctttcg ttgtcgaaaa ggtctggtat accttatgtc gaaggaaagg
    12601 aaacaaaaaa actttcctgt actattccaa aaaggaaaga aagttattat acagtaaaaa
    12661 aagaaattat tcttcatgct agagatcagt acacgttcga gcccaatatc aaacactagt
    12721 tccaggcagg cgcaacaggt gcggttatca ctgaattttc ttccagcagt gacgattcaa
    12781 gtgatatttt tacaaatcca gctatttatc gttaacaaaa agatttttag gataagctga
    12841 tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg cttttttatt tagagaaaca
    12901 tcatttatca gtgaaacata acacgagaac aaaatgattt tttcgttaat aaaagaaaca
    12961 agtatttgtt gtttaaaata atacacgtta ttggcatata ggacgaaatc ccagtatctt
    13021 ctataagtaa gtcatttcat atcgatgaaa acacgttatt tatttttgtt ttatctataa
    13081 aatagctgaa aaattatttt ttctaaatac tatatatagt ggtgtctatc ctatatacca
    13141 ctaaaaaact acaaaatcta gtcttttatt ttaaaaaaac acgtgtagaa tgaagataga
    13201 cttttgagtt agggagtgtt tttttatgaa atttactgtt ttttgcggtt cccgttttgg
    13261 taataaagaa agttacaaat ttattgcgca aacgttagga aaatacatgg cacaagaaga
    13321 aatcgaactg gtttacggtg gttcatactc cgggattatg ggagtgatta gtaaaacagt
    13381 actggaaaat aatggaaaag tcacaggtat ctatccgaat gggctttttg aaactgaatt
    13441 gcctggaaaa gatgttacta catttattcc tacagaaacg atcgatgaaa gaaaggtatt
    13501 attatttgaa aagggagatg ctgtcctcgt tttccctgga ggtctaggta cattggagga
    13561 attctcacaa cttctttctt ggatagcaat cggtctgacg cctgacaagc caatcggaat
    13621 attgaatatt ggcggttatt acagcggctt gcaacaatta ttagaaactt ttgcaaaaga
    13681 ggattttatg gataaaaaat ggttggatca agtaatcttt tccaacaatc cattggaact
    13741 ggtcaatgaa ttacgagcag tatctattaa aaatcagtta ttattgaagg aggcaaaata
    13801 atgagtcaaa tatatttagc aggaccattt ttttcagagg agcaaatcga tcgagtaagt
    13861 cgtatcgaaa aagcgttgga ggaaaacaaa acagtcacaa gtttctactc tccacgtcat
    13921 caccaagaaa gcaattatga actattcagc gctggatggg cgcaggaagt atacgaaaaa
    13981 gatatggaag aactgacaaa tgcagaattt gttgttgcta tccttgactt tgagcatcaa
    14041 acaatcgatc caggaacggc ttacgaatta ggtgtggcaa ctatgttgaa aaaaccgatg
    14101 atcattgtac aagaagagac ggtacctacg aacttgatga ttacacaaag tctccatacg
    14161 tatttaaaat cagaccaagc tgtacgtgaa tatgattttg aaacattgcc tgttgaaact
    14221 tacgtaggcg agtacttata gaagaggaag ggttgtatgt cagtgatttt gatttcagga
    14281 acgattggtg caggtaagag cagtttgacg gatatgcttg caaaagagat tgattctaaa
    14341 ccattttatg aaaatgtcga agataacgaa gtattgccgc tcttttattc aaatcctgag
    14401 cagtacgctt ttttattgca aatcttcttt ttgaacaagc gatttttagc aatgaaaaat
    14461 gctttagtga acgatgacaa tgtgctagac cgatccatct atgaagatag cctgttgttc
    14521 catctaaacg ctgatttagg tcgggtaacg gatatcgaag tacagcagta cgatagctta
    14581 ttagatacga tgctaaacga gttagatgat gttgctccaa aaaaacggcc tgatttgatg
    14641 gtccatatca aagtttcttt ggacacgatg ctagaacgta tcaaaaaacg tgggcgtgac
    14701 tatgaacagc tagaaagcga tgagacgtta tatacctatt atgaaacgtt gaacacccgc
    14761 tataatcaat ggtatgaaga ttttgatgta tgtcctaaaa tacaagtgga tggggataaa
    14821 ttcgattttg tagaaaatcc agaagatgca aaaaaggtga tccatcagat ccaagaaaaa
    14881 ctagctgaat tggaaggaaa gcctgctgga aaatatttta cggataacca gcatgcaaaa
    14941 ggtcaagaat taggctaaaa ataaaaaacc aaacgacggg aaagaaaaat tactacgtga
    15001 tgtagaataa gcctttctat cgtttggtta ttttatattc aaatatttga aataaataaa
    15061 tctttactct ttgatttgta aactgaaatg atccactgtt tgttttactg atgaagcttg
    15121 atcaaaggaa gaagctgagt ttgaagttca taaaaatcag ccatagtgag ttttgcttgg
    15181 atttgtttat cttcttctac tttttacata attttgaaca ggataagatt caaatgtttt
    15241 aaatgaatct ttgactagtt tgtgacagtt gtcgcatatg ctcaagcttc cttggtcatt
    15301 aaattctgta tggatatttt tgttgttatt acatgtacaa taatcaggac tatcgatcat
    15361 ccatttcgtc tcctttacaa tcatcttgct tctttactat agcagaaaag aaggggagaa
    15421 acaaaagaag atccaacaga cggcactata gactgtagat gttgccatcc tcatccttca
    15481 aacggtaatt accgattttg gtaaatgtga tggttgcttc ttttttattt tcttttcctg
    15541 aaataatata ttcgtcatct ttggtagtaa tcttgatgtc tgtcagcact gttttttctt
    15601 cagatgattc agaagaatca gtttcttgtg tctgatacgt gcgtgtccat tccatttttt
    15661 tatctgtaaa tgtcaactgt cccgcggata aatccatatc tttacgtact tttagcgtat
    15721 atacactgtt agtgatatct gctttttccg cttttaagac agaagaatcg gttgtttcat
    15781 ccgaggattg gtcagagccg ccaaacaaat tacagccagt caatgtcaaa agtcccgcag
    15841 acaaaataaa tagtacgatt aatttcttca aaatacttcc cccatttgat aggataaaga
    15901 aattatacca ttggaaagta aaacttcaaa cggatagaag agaaaaacgc acaaaacacc
    15961 cacaaataga gtaaatttta gaataaaatt ttaaaaaggc aaaaaattcg ccattttagg
    16021 aaagcgtttt aaaaaagtgt tgcaatcttt taaaaaaatg ctatattaaa aatgaaccgg
    16081 ttcaaatact tgtttaagta taattaatag tgagaggaag ttgctctact gaagacttta
    16141 gttatatgga tattgatgac aagcaacact atgcaaagaa agcggtcatc ataatcgaac
    16201 aacaagaaag agaaatcaaa tgtaactggc aggagtgaga atgtatggca tcgatccgtg
    16261 acgtggctaa gatggctggt gtttcagtgg gaacagtttc ccgttacttg aatggtcagc
    16321 aactgaagga aaagaacatg aaaaaaatag cagaagcgat ttcagcgttg gactataaag
    16381 aaaacatcat tgctaaaggg ttgaagaata atcgaagctt ctctattgga cttttgataa
    16441 acgggatttc cagccgtttt gggtcggaag ttgtctcagg gatcgaaaga gtagctgaag
    16501 aaaatgggta tagcttatta ttaagcgggt ttgccgatca gccagaaaaa attgaacaaa
    16561 aaattgaata cctgatgaag catgtgatcg atgggctgat tgtcttctta tccgaagaag
    16621 agtggtctgg atttgaaatg ctcacaaaaa tgagtattcc agttatcgca ttgaattgcc
    16681 cagaaggacc agcaggtatt gatacaatat tagtgaatga tcgagaaagt gtagcaaaag
    16741 tgatcgctca tcatggtgaa caaggacata aaaaaatcgg atttattgct gccactcaaa
    16801 cagactacgt agcaagagaa cgtttagcag gagcaaaaga agcggtacaa aatgatccat
    16861 cgatccaatt agaggtcttt acaggagact attctagaaa aagtggctat tatggggcaa
    16921 aagaattatt agaaaaagga gtaacagcga tttttgtcag caattataat atgtcgatcg
    16981 gtgctatcga atgtttcaat caagaaggta tcagaatcgg gaaggatatt gcgtttagtc
    17041 attatgatta tttggataaa aacgatttga gcatccttcc gaaaatcacg attacaccac
    17101 caacagataa aatcggggaa caagccgcag aacagctgtt gaaacgaatc aaaaaagaag
    17161 aggaagaaaa tgaaaaagta attgtgatgg aaaatatcat acatggaatc aactaaaacg
    17221 aactaaaaaa gaaaaagagg tgctttgatg aaaaaatatc aatttccagc aaatttttgg
    17281 tggggttctg cagcaagtgg cccacaaaca gaaggaagga tttcaggtga tggaaaagga
    17341 gagaacattt ttgattattg gtacaaaaaa gaaccgcaaa agttctttga ccaagtagga
    17401 cctgaaaaaa cttcacaagt ttatacgaag tatcaagagg atgtacagtt gatgaaacag
    17461 actgggcata attcatttcg aacatctatc caatggagcc gcttgatacc tgatgaatct
    17521 ggaacaatca acgaagaagc cgttcgtttc tatcatcgtt attttgatga gttgattgaa
    17581 aacggtatcg aaccctttgt gaatctgtat catttcgata tgccgatgta tctgcaagaa
    17641 aaaggcggat ggttgaaccg agaaacggtt gaagcctatg aaaaatatgc caaaagttgt
    17701 tttgaattgt tcggttcgaa agtaaaaaaa tggtttactc ataatgaacc aatcgttcca
    17761 gtcgaagcag gttatttata tcgatggcat tatccagaag agtcagatat gaaaaaagcg
    17821 atccaagttg gctatcatga agcgttggcc tcagcgttag ccatcaaatc ttaccatgaa
    17881 atggagcaag gtggtgttat tggtatcatc ttgaatctga caccaagcta tccaagagac
    17941 gaatcaaacc ctgaagatgt taaagcagcg caaatagcag atgcgttctt caatcgttca
    18001 ttccttgatc cagctgtaaa aggcgagttt ccaagtgaat tgattgagat agtaaaagaa
    18061 cttgatctca tgcctgtgat ccatccagag gatttgacgg tcatcaaaga aaatacagta
    18121 gatcttttag gtatcaatta ttaccagcca cgccgggtaa aagcaaaaga aacaccaatc
    18181 gatactaaaa acggtccaat gcctgaggat tatttcgaca attatgaaat gcctggtcgt
    18241 aaaatgaatc cttatcgcgg atgggagatc tatgaacaag gaatctatga tattttgatc
    18301 aatgtacgag atcattatgg aaatatcgac tgctttattt ctgaaaacgg tatgggtgta
    18361 gaaaacgaag cacgatttat caaagaagat ggcatgatcc acgatgatta tcggatcgaa
    18421 tttgtccaat ctcatctgaa atatgtccat caagcaattc aagaaggtgc caattgttta
    18481 ggctatcata tgtggacatg tatggataac tggtcatgga ccaatgcata taagaatcga
    18541 tatggcttta tttcagtaga cttagcacaa gatggaaaaa gaacaatcaa aaaatcgggt
    18601 cactggttca aagacgtatc agataaaaac ggatttgagg cttaagaaag aatagttgag
    18661 agcaaatgcg tgaaattctc cattcttacc attttgtgaa aaagaagttc aattatttac
    18721 gatctttttc aacttttttg tctttctatt tcattttttg tatataatag aatagtcaaa
    18781 attaaatagg agtggaggaa agcatgagga aaaaaagacc gtttcttctc ttttggttat
    18841 tcggtttttt cttactgctt attttatccg cagcattttc aggaaaaatg acagcagatc
    18901 ttgctcagaa agaaacagaa aaaatcgtag caagcaaaga atcagtagat aatttcaaaa
    18961 aagaattctc aacttattgg tgggcggagt ataaaataac tgccaattac gaaaaaggcg
    19021 aattggacat ctatttgcca aaaggaaaag aagaaaatca atttattaat atcgatgacg
    19081 tacttagtgc gatttctctc agacaatatc aatttaaaga caagcatttg accgttttgc
    19141 ttttgactca tgatggcgac aaagtagccc gtttgaaaaa tggaaaagtg atcaccgctt
    19201 attctgaaaa agtagacaaa aaagtggtcg ccgatcgttt agcggaatgg aaaaagacaa
    19261 atgaacaagt caagaaaaca actgtctctt ctcaaaaaaa cgagtcgaca ggaacaagtc
    19321 cttcagattc tggcagtgga caaacgataa cgggaagtga agaaaatcaa gcctcaatta
    19381 ttacgaatga gtcatcacaa ggacaatatc aatcctatgg acaatgaaaa a
//
LOCUS       DACDYJ010000049        19129 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDYJ010000049 DACDYJ010000000
VERSION     DACDYJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.049"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            647..964
                     /locus_tag="IWM51_001852"
     CDS             647..964
                     /locus_tag="IWM51_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9971269.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1055..1420)
                     /locus_tag="IWM51_001853"
     CDS             complement(1055..1420)
                     /locus_tag="IWM51_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971270.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1628..2110
                     /locus_tag="IWM51_001854"
     CDS             1628..2110
                     /locus_tag="IWM51_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971271.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2307..3197
                     /gene="pstS"
                     /locus_tag="IWM51_001855"
     CDS             2307..3197
                     /gene="pstS"
                     /locus_tag="IWM51_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP9971272.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(3484..4758)
                     /locus_tag="IWM51_001856"
     CDS             complement(3484..4758)
                     /locus_tag="IWM51_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9971273.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            5031..7502
                     /locus_tag="IWM51_001857"
     CDS             5031..7502
                     /locus_tag="IWM51_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9971274.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(7605..9341)
                     /locus_tag="IWM51_001858"
     CDS             complement(7605..9341)
                     /locus_tag="IWM51_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP9971275.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(9338..10042)
                     /locus_tag="IWM51_001859"
     CDS             complement(9338..10042)
                     /locus_tag="IWM51_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9971276.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            10173..10676
                     /locus_tag="IWM51_001860"
     CDS             10173..10676
                     /locus_tag="IWM51_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP9971277.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            10636..10974
                     /locus_tag="IWM51_001861"
     CDS             10636..10974
                     /locus_tag="IWM51_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP9971278.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10995..12413
                     /gene="ffh"
                     /locus_tag="IWM51_001862"
     CDS             10995..12413
                     /gene="ffh"
                     /locus_tag="IWM51_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP9971279.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            12525..13103
                     /locus_tag="IWM51_001863"
     CDS             12525..13103
                     /locus_tag="IWM51_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP9971280.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            13107..13628
                     /locus_tag="IWM51_001864"
     CDS             13107..13628
                     /locus_tag="IWM51_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9971281.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(13707..14261)
                     /locus_tag="IWM51_001865"
     CDS             complement(13707..14261)
                     /locus_tag="IWM51_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9971282.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            14514..14789
                     /gene="rpsP"
                     /locus_tag="IWM51_001866"
     CDS             14514..14789
                     /gene="rpsP"
                     /locus_tag="IWM51_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP9971283.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            14801..15052
                     /locus_tag="IWM51_001867"
     CDS             14801..15052
                     /locus_tag="IWM51_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9971284.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            15177..15968
                     /locus_tag="IWM51_001868"
     CDS             15177..15968
                     /locus_tag="IWM51_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971285.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            16138..16659
                     /gene="rimM"
                     /locus_tag="IWM51_001869"
     CDS             16138..16659
                     /gene="rimM"
                     /locus_tag="IWM51_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP9971286.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            16649..17410
                     /gene="trmD"
                     /locus_tag="IWM51_001870"
     CDS             16649..17410
                     /gene="trmD"
                     /locus_tag="IWM51_001870"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP9971287.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            17546..17893
                     /gene="rplS"
                     /locus_tag="IWM51_001871"
     CDS             17546..17893
                     /gene="rplS"
                     /locus_tag="IWM51_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP9971288.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            18151..18444
                     /locus_tag="IWM51_001872"
     CDS             18151..18444
                     /locus_tag="IWM51_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP9971289.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         6362 a         3368 c         3825 g         5574 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga
     1021 aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag
     1081 ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa
     1141 gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg
     1201 cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa gatataatac
     1261 gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc
     1321 cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag
     1381 ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa
     1441 gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc
     1501 attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc
     1561 cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg
     1621 aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc
     1681 tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg
     1741 cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta
     1801 tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca
     1861 gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt
     1921 gattatcggc cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat
     1981 gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac
     2041 atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa
     2101 taactattga tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc
     2161 tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat
     2221 aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta
     2281 gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg
     2341 gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa
     2401 ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac
     2461 agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct
     2521 ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta
     2581 tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg
     2641 atcataaagt agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag
     2701 atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag
     2761 ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg
     2821 cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt
     2881 catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt
     2941 tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag
     3001 agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa
     3061 aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag
     3121 gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg
     3181 aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa
     3241 aatcaactat gtttccccac atatacattt ttcctttaat tgtgaaaaat agttcctcat
     3301 attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt ttattcgaaa
     3361 aaaagagcta cgacatagaa ctgtcgtaac tttttttggc tcttcgtcaa atcgtgttgg
     3421 tgaagacaaa tttgaataaa acttagtccg atatgcggcg cgttctgtgc gccgcatatt
     3481 tttttagttt tcaaagatca gaccttgttg taaatagatc cgtgcccgaa agttataaaa
     3541 gttgcgataa ccaaacgcaa ttcgtttaat cagcttgatc ttgttattca gcccctcagt
     3601 gacgccgttt gagtgggaca ccttaaaggc atttgaaata cccgattggt agcaatggaa
     3661 gatcgtgaat ttctttttaa acgactgagg taacgtagat gaaattgttt taagggtttc
     3721 ttgaaagcct tccgtacctc gtttaacgaa ataatagcgt aataattgac aaaaatgata
     3781 cgcagttcgc aattcttcgt tgtagcttaa cagctcatca acgatttctg tttgactaat
     3841 tggtcgttta aacagtcgat gataatgccg tttgagtggc tcccatgtcg tggaatcttt
     3901 gagtaagagt ttccaatatc gcttgagccg acggtacttc ttctgatttt cagagtgtga
     3961 ggttcgaaaa cgattcatga tctggacacg taattgattg aagctgcgat tgatgtgttg
     4021 gacaatatga aagcggtcag ttacgatctc cgcatttgga aagactgttt taagcaactg
     4081 acaatagctg gcattcatgt ccatcactag gtacttgacc tttaaacggg cctttctagg
     4141 aaagcgcagg aagtattgag ttagtttgta aagtctgcga tcttcaagga tcgtaaatag
     4201 ctgattggtt gtcccatcaa cacaaataaa actcatggaa ccagaaacag aattcattga
     4261 tttgaactca tccatacaca acaccgtcgg aaggaagttc atacgtggct ggtaatgctt
     4321 cgttgcttca cgcaagatcc gtgtcaccgt cccatccgag acgaagtact tcttagcgat
     4381 tagtttacgt gattgatttt cgtacagttc catgagaatc gcctgcttca gttcctttgt
     4441 tagttgatga tgttcatcaa ctaagtccgt tttcgcagaa aaggtccttc cacaatcatg
     4501 gcagagaaaa cgggtacgtt ttaattctaa aatagttaac ttttctttga ctttcaacat
     4561 ctgagtgcgt gtcgtatagg aaccgttgcg gataatacga accgatcgac agtgttcaca
     4621 ataggaagaa gagtcatcca attttccttt aataaactgc gcttgaacgc ctttatattc
     4681 tttttcaatc agccattctt catcaaaaaa taaatgttta tctgttaatc cgagtaattt
     4741 tctagtatga ttatccattg agagactcct ttggtaggat gacgttttgg tcgactaaat
     4801 tttaccaaaa gtgagtctct ttttgagttg aaacgaagaa aaagtgttag tagaaaatcg
     4861 ccttcgattt tccaccaaca caaaatatta tagaaccctt tttttttgct atatgacagg
     4921 aatgaaaggt gtttgcaagc ttaatgtgag gaacactttc catatctgtt ctatactgga
     4981 taggagaagt ttggaaatat ccagaaaacg aaatcaatcg aggtagccac atgaagaata
     5041 aatggataaa tcgaagtaaa aaaaagaaag taacacaaag atcatcagat aaagtcatcc
     5101 gtctttttcc atcgtataaa gaaggattaa cggcagatca agtcaaggaa cgaatagaaa
     5161 aaggtgcagc aaataattca gtcgatccca cattcaaaac aaatcagcaa atcgtattgg
     5221 aaaacatatt tacgtatttc aatttgatct ttcttatctt agccattttg ctttgtttag
     5281 tagaatccta taagaacttg acttttttac cagttatcat agccaataca ggaatcgcca
     5341 tttttcagga aattcgatcc aaaaagatct tggacgaact aaatgtttta aatacagcaa
     5401 aagtcaatgt catacgagac gggatagaac aaacaataga tatagaagac cttgtaatag
     5461 atgacattgt tattttagag actggatatc aaattccagc agatgcagag gtcgtggaag
     5521 ggaaactgca agttaatgag gctttattga caggcgaagc agatgaaata accaaagaaa
     5581 taggtgatcc attaatgtcc ggcagtttca ttgtttccgg aaaagcttac gcccgattag
     5641 acaaagttgg agcagattcg tatatttccc agctgacagt caaagccaag acaatgggag
     5701 aaggagaaca gtcggagatg attgcttctc ttaatcaatt gatcaaatgg attgggatca
     5761 tcattattcc tatcggtatc ctgctttttt ctcaaagtta cttttttaat ggaaacacga
     5821 tcaaagaaag tgtcgttgca atggaagcag cattgatcgg tatgatacct gaaggactgt
     5881 acttgctgac gacgattgca ttagcattaa gtgcgactaa actagcaaag caaagagttc
     5941 ttttacacaa tatgaaaagc attgagacat tggcaagggt aaatgtcctt tgtgtagata
     6001 aaacaggaac gatcacagaa aataaaatgt ctgttcaaaa aatgatcgtc tctaaaaaac
     6061 aagaaataat cagtcagtca gatcaattgg aagcatgcat tgctgactat gcaaaagcga
     6121 tggcaggaga caatgcaaca atggaagctg ttaaagcata ttttacaaaa accactgatc
     6181 aatcctatca ccatgttatt cctttttctt ctgtccaaaa attcagcagt gtagcgttgg
     6241 cagacaaggt gtatgtttta ggagcgcccg aaatggtttt gcgcgatcaa ttgccggaat
     6301 atgcggaaga gtttgtttct ttcgctgaaa aaggctatcg cgtgttggtc tttggtgtgt
     6361 acgatgggat tcttgaagaa ccagtattga cagaagcagt cacaccatta ggctatattc
     6421 tgattgctaa tcctatcaga aaagaagcaa gagccacatt tgattacttc aaaaaacagc
     6481 aagtagccat caaagtgatt tctggagata atccgctcac cgtatccaat gtagctgtcc
     6541 aagcagggat ttcacatgct gatcagtacg ttgatgcatc taaattatca gaagaagaat
     6601 atgaagcagc tgtcgaaaaa tatacagttt ttggacgcgt caagccagaa caaaagaaaa
     6661 tcttcgttca attgataaag cgtaacaata cagtagctat gacaggtgat ggcgtgaatg
     6721 atattctagc catgaaagaa gcagactgca gtatagcgat ggcttccgga aatgaagccg
     6781 ccatgcaggc ctcacaagtg gtcttacttg attctgactt ttcacgcatg ccagaagttg
     6841 ttgcagaagg acggcgcgtt gtgaacaata ttgagcgatc agccagcttg tttttagtga
     6901 agaatatttt ctcctttttg ctctcactgt tttcagtgat ctttgctctg acctatcctt
     6961 tagaaccatc acaaatcaca ttgattagtt tgtttactat cggacttcca tctttcttgc
     7021 ttgctctaga agaaaacaaa aagcgaatca gagggaaatt catcatgaat gtgatggaaa
     7081 aagctgtgcc aggtggacta acagatatga tcgtagtcgg tgcgttggtg atctgcgggg
     7141 tgactttgaa tctaaataaa acagatgttt ctacagcatc taccatgctt ttgattgcag
     7201 tagggttcct cgttctctat aaaatctgtt caccgctgaa taaattcaga agtcagatca
     7261 ttcttttctg tgctagcggt aacttttttt cggtcatctt tttgcataag ctgttttcaa
     7321 tcaccggtat ctcagctgta tcacttttgc tgttgagcat tctatttttc tcggcagatt
     7381 cgatttttag gcatttgacc acattggtag aatatttgtt tgaacgtaaa aatgaaaaag
     7441 agcaaaagcc gtttagtcgc gtagctaaaa aatttctatc gatatttaaa cgagaaaatt
     7501 gacgaaaagt atctcaaaaa agagtaaaaa agaacaacca ctcgtgtatc tttcgaagca
     7561 ggatgagtca tccaacttta aaacgcacga gcagttgctc ttttttacat tttcggaatg
     7621 gtaacggtga aagtcgaacc tgttccaaga tgactgtcta cagtaatcgt accattaagg
     7681 agttgcacgt agtcttttac aatcgctaac cctaatccgg tcccgcctga atgtcggctt
     7741 cgcgccttgt ctacgcggta aaaacgctca aagatccgtt cctgatcctc aatgtcgata
     7801 cctataccaa agtcttggac agaaaacgat agatcatctg ttgcctgata gcggataata
     7861 atctcgctgt cgctttttga atattggaca gcattttcaa tcagattttt gataattgga
     7921 taaaggatat ctgttttagt agtaaatggt tcgttctttc caatcaatcg aatcgtcaac
     7981 cgtttctttt cgatgattgg ctgataattt tgaacgattt gctggaaata aggttctgta
     8041 taaattgtct ggatctcgta agaattctcg ccatcttttg acagctggat gatttcccga
     8101 atcagtttat ctaaccggat cgcatctttt tgcatgatct caagaaaaga agtcagagtt
     8161 tgcgggtcat cttttgcacc atccagtaaa gtctcggtaa aaccaatcag tgaagtcaca
     8221 ggagttttta attcgtgaga gacgttgcca acgaaatcct tttgtagctt ttctaaacgt
     8281 cgcactttcg ttaagtcata cgccacccct agaatctgtc cggtaccttc tgaattagta
     8341 aagtagcgca aactcaagtc taatgtctgc cgaccttctg taagggtgat ctcctgatgg
     8401 atcagcggat gctcaggagt cacttgatga atcagctgaa tgagttttgg ctcttgaatg
     8461 acttctgtgt accgctgttc aggttgatag cttaccacgt ttaaatgtag ctgcatcttt
     8521 ggattaagca gtagtaaacg agaatcagca tcatcaatca ggaatacgcc aatcatcagt
     8581 tcgttcagta acgtatataa ttgctcttct gtggtagtac acgcccgata catcttactc
     8641 atttgttcgc tcaacgcatt aatcgtctga tacaattctt cccattgatc gggtgtttgc
     8701 atgattactt ccgcctgatt aggattttgc accatttttt ttagtacggg cagcacggtt
     8761 ttcaatggct ggtttttttg atagatcaga taatagatca ttcctgcaag aacaaggaag
     8821 aaagacagaa agaagaaaaa gacccaacgg cgaaaactct cagttctcgg taaaaaaacg
     8881 cttgtcggtt ctgcgatacg taaaatggct tctaactggc cacttttttt gactggtaat
     8941 gcgacatata aaagatcctc attcaaagta gtacttttgc gcaaagcgct acctaggttc
     9001 ccaccattca atacggcttt gatttcggga cgggagtttc gctgttcatt caatgtttca
     9061 ttgtttgtgt caaataagat ctttccagta gcatccatta aagtaatccg ttcgttcgac
     9121 tgatggacga attcttccaa agcagttttg ttttgcgctg cttggaggtt ttcgacatct
     9181 aattgccgga tcagcagttg tgcttttttt gtcaggtagc tttcttgctg ttccacgatc
     9241 tgctgttgaa aataatggga aatcaattgc cagccaccaa aaaacaaact aactaaaaga
     9301 ataagaaaga agagacgttc ataaattttt ttccatttca tcgtttgggt tcctgaaact
     9361 tatagccaaa tcctctaata gtcaccaagt actttgggtg ttttggatct cgctcgattt
     9421 tttctcgcaa atgactaacg tgtacatcta cgattcggct ttgtcctgcg aagtcaaaat
     9481 tccaaatgcg gtctagcaat gtgtctcggt cgatcacacg gtcttttcgt ttcatgaaat
     9541 aaacaagtaa ttcaaattct ttcggtgtta gttcaatcag atggtcgtct acggtcactt
     9601 gataattggt caaatcagca gtgatttgtc caatcgatag atattcaggc tcagcttcat
     9661 cttttgattg cttacgaggt tcaattcgtc tgaatattgc tttcatacgg gctaatactt
     9721 cgcgaggact aaatggtttg gtcaaatagt cgtcagcacc tatttctagg ccaatgatcc
     9781 gatccacttg gtcatcttta gccgtcagca tcaaaatcgg tgtttcattt ttttcttgac
     9841 gcagccgctg agtgatctcc atgccatcca tcgttggaag catcacatct aaaatgatga
     9901 aatcaaatga ttgttctaat gctaattcta atccttcgcc tccatctgtg gcactggtta
     9961 ccttgtagcc ttctttttcc aaattgaacg tcaagagcgt tacaatagaa ggttcatcat
    10021 caacaactag tatttttttc attcaaatct cctttaaata ggtttcattc tgtttatatt
    10081 ttagcataga atcagtacaa agggctattt tacaaaagaa ataaatggta taataggaag
    10141 gcactttgct gaatagaaag gaagagcagt caatgattgg aatcagtgca tgtttaggcg
    10201 gtctggcctg tcgctatgat ggacagaaaa aagaagtacc agaattgatg gaactagttg
    10261 aaactggaga agctgtcatg gtctgtccag aagtgatcgg cggattacca attcctagag
    10321 atcccgctga aatcattggt ggaaatggat tcgatgtgtg gaaagatcaa gcaaaagtat
    10381 ggacgacaag cggcgaagat gtaacggaag cgtacaaaaa tggcgcgatc aaagcctatc
    10441 aaaaattgca ggaaaaacac attgatctag tcatcatgaa ggaaaaaagc ccttcatgcg
    10501 gaacccattc gatttatgac ggaagttttt ccggaataaa aaaaaatggt gccggagtgg
    10561 ctactgctta ttttattcag caaaaaatga ctgtgctctc tgatgaagaa tggctgaaaa
    10621 ctagaggtga gcagaatgga aatcgaaaag accaatcgaa tgaacgcgct ctttgaattt
    10681 tattcgacct tattgacaga aaaacaaatg aactacatgg aattatatta tgcagatgat
    10741 ttttcattag gtgagatcgc ggaagaatac gaagtaagcc gacaagcagt atatgacaat
    10801 atcaagcgga caagcaagat cttggaggat tatgaaaaaa aactccatct tttttctaat
    10861 tatattgttc gagaacaggt attggaaaat atgactgcat atattacaga taagtacccg
    10921 gaagataaaa aactacttga atatgtacag caaattcaag caatcgaaga ataaataaaa
    10981 ggaatgaaga aaatatggca tttgaaagct taactgaacg tctccagcag gcgatgggaa
    11041 aaataagaaa aaaaggaaaa gtatcagaag ctgacgtaaa agaaatgatg cgggaaatcc
    11101 gccttgcctt actagaagcc gatgttaact tgcaagtagt caaagatttt actaagcgcg
    11161 tacgcgaacg tgcggtaggg gcagaagtgc tggaaagttt atcacctgca caacaaatcg
    11221 taaaaatcgt tgatgaagaa ttgacgatca cacttggttc tgaaacagca gagttaaata
    11281 aatcaccaaa aataccaact gttatcatga tggctggtct tcaaggggca ggtaaaacga
    11341 cctttactgg taaattagcc aattatttaa agaaaaatga aaatgctcgt ccgctcctaa
    11401 tcgcagggga cgtatatcga ccagctgcga tcgaccaatt gaaagtatta ggtcaacagc
    11461 tagacgttcc tgtatttgat atgggaactg aagtcagccc ggtagagatc gtccgtcaag
    11521 ggatggagtt agcaaaagaa aagaaaaacg actatgtatt gatcgatacg gcaggacgtc
    11581 tgcatatcga tgaaacattg atggatgagt tgaaacaaat caaagaattg acgcaaccaa
    11641 atgagatttt gcttgttgtc gatgccatga ctggtcaaga tgccgtcaac gtagctgata
    11701 gcttcaacca gcaattaggt attacagggg tcgtcatcac taaacttgat ggggatactc
    11761 gaggcggggc agcactatcg atccgctcag tgacaggcgc accaatcaaa ttcatcggtt
    11821 ctggtgaaaa attgactgac ttagaagtat tccacccaga tcgtatggct agccgtatcc
    11881 ttggtatggg ggatatgctg acattgattg aaaaagcgca gcaagactac gacgagaaaa
    11941 aagcggaaga acttgcacag aaaatgcggg aaaattcatt cgatttcaat gactttattg
    12001 aacagcttga tcaagtgatg ggtatgggcc cactggaaga tctaatcaaa atgatcccag
    12061 gaatgaatca agtccctgga atcgaaaacg tcaaggtaga tccaaaagat gttgaacgca
    12121 aaaaagcgat ggtttattct atgacgccag cagaaagagc aaatcctgat ctgttgaacc
    12181 caagccgtcg tcgccgaatt gctgccggtt ctggaaacag tgttgttgaa gtgaatcgaa
    12241 tgatcaaaca attcaaggaa tcaagaaaaa tgatgcagca aatgtccaaa ggggatatga
    12301 acatcccagg tatggaccaa atgtttggtt caggcgtcaa aggaaaacta ggcaaaatgg
    12361 cgatgaatcg aatgatcaag aaaaataaga agaaaaagaa aaaacggaaa taagatgaaa
    12421 aaaacagctt cggtacagac tgaaaatgtc tactgccgga gcttttctat cagcaatttt
    12481 tattctttac taatacaatt actctaaacg aagaggtggc acagatgaga tgtccgtgga
    12541 gtgaacaaac agaaagtatg cggcaatatc atgatactgt ctggggtgtt cctgaatatg
    12601 atgaccaaaa gctgttccga aaattgatgc ttgatattaa tcaagccgga ttgagctggc
    12661 agacgatttt aaataaaagt gaagcattcg atgaagcata tgatcgattc gatatcaaaa
    12721 aagtcgcaaa ttatggagaa acaaaaatca gtgaactaat ggaaaacaaa ggaattattc
    12781 gtaaccgaag aaaaatcgaa gcagccgtcc aaaacgccaa agcagtcttg aaaattcaag
    12841 aagaaatcgg cagtttttca gagtatcttt ggtcgttttc taatggaaaa atcgttgatt
    12901 cttcttatca ttcacaagaa gaagtcccaa caacaagtcc tctttcagat gaattagcaa
    12961 aagatctaaa gaaaagagga tttaaattta tcggaagcac aacaatctat gcttttttag
    13021 aagctgtcgg gatcatcaat gatcaccttg acagttgttt ccgcaaagaa gaggtcgtac
    13081 gtatcggcag aaagggcgaa taaagcatgc gaaaaatcat attttatggt gctataagtt
    13141 tagatggcta tttggcaacg aaaacggatg atcttcaatg gctttttgac actccaacag
    13201 gtaagcaaac tacttatgaa gctttttatg aaacaatcga tacgaccatc atgggcagaa
    13261 aaacttatca ggaagcaaaa aaatatttgg agaccgaagc aatttatcca gagaaaaaga
    13321 atttcgtttt ttccacccac gcacaattac aattgccaga tgctgaggtc gtaaacgaag
    13381 atcctgttgc ttttctaaaa aatctacaaa tgacagaagg aaaagatatc tgggttgtgg
    13441 gtggcggtcg attgatcaaa ccgctattgg aacatcaaat gattgatgaa tggtatatcc
    13501 agattacccc tgtattatta ggggacggga ttcctttatt ccaaaaagga acatatgaag
    13561 cgcgttttga aatgctagac acgacacgat tcggtgaatt tatcgaattg cattatgtac
    13621 gaaagtagaa gtaaataaaa taaaaaaggc aactatatgc agaaatgtcc tgtacatagt
    13681 tgcctttttt attaccgtta tgctttttac tctgttaatt tgttagaaaa atctagatac
    13741 gcatctaaaa tcgtttggaa gaaacttaat gttccttcaa cgatattacc attttcatca
    13801 attagattcg caacgtttcc gatatatgct tccggttgct gcaaggttgg cacattcaaa
    13861 aagacaagtg attggcgtag atggtggttt gcgccaaagc cgccgatccc acctggtgat
    13921 gcagtaacga ctaaacctgg tttatgatcc caaacactgt gtccataagg tcttgagcct
    13981 acatctaaag catttttcaa tactgctggc acagaacggt tgtattcagg agtaacgaaa
    14041 acgacaccgt ctagtcccct cacttcctca cgaaaacgag tccattcagc aggagcctgt
    14101 tctggagttt cgagatcctc attgtaaaat ggcagatcat cgatttttac aaatacactt
    14161 tcgtaccctt caggaagcaa tttcgcaaaa gcttctgcta cttttttgtt gtaagaatct
    14221 tttcttaaac taccaataaa aaatccaatt ttttttgtca tacgattttt cctccttcaa
    14281 actatctata ataatcttac aaaagataag taactggagc aaataaaaac acttactgac
    14341 aaaagctaca tagaagatag attacttatt tttaacaaaa aagaagtcta aaatcttcta
    14401 ttttctaaaa aaaatcgaat ttaatcaagc tgtaaagaaa aaaagcttta cagggtaaaa
    14461 aaaaactgtt acactacatc ttgtgaataa aacaatggag gtggaaataa ataatggcag
    14521 ttaaaatccg tttaaaacgt atgggttcta aaaagagtcc gttttaccgt atcgtcgtag
    14581 ctgattcacg ttctcctcgt gatggacgtt tcatcgaaac tgtaggtact tacaatcctt
    14641 tgaaagaccc tgcagaagta gttttaaaag aagacttagt tcttgactgg ttatcaaaag
    14701 gtgcacaacc ttctgacact gtacgtaaca tcctttcaaa agaaggcgtt atgcaaaaac
    14761 accacgaagc taaattctca aagaaataag gtgaccggat atgaaagatt taagcgactt
    14821 agtcttaaca atcgttcgtc cgctagtcac ttatcctgat caagtcaagt tagacgtcgt
    14881 agagtcgaaa gacttttacg agtacaatct aacagtggct cctgaagata ttggccgtat
    14941 cattggaaaa caagggcgtg ttgctaaagc aatccgcaca atcgtttacg gtgttcggat
    15001 caatgcaccg aaaaaagtgc gtttgaatat catcgatgat aaggaaaagt aaataaacgt
    15061 gctgtactcg aattttcgag tgcagctttt ttgttttttt tattaattga agaaatgatt
    15121 atttggcaaa acaagcaaga aagcgtatcc ttataggagg agggagtgaa cagccaatga
    15181 gaaagaaaat gttatttatt ccatttgctc tattgttaag cgcttgtagt tcggttactc
    15241 aagaatcaga caatacacat ctatcaagca gtatatctga aactgttacc agtccagaaa
    15301 aaagtagtac caaaacggct tccaccactg aacaaacaat ggctacctca aataatgaga
    15361 aaaagactgc tttagaccag ttgaaagaac aacagccaaa tgttcccatg cctctagatg
    15421 taccagtctc ttcaggctat ctcaatatcg cagcgacaca tacaaagcaa ggttattcta
    15481 ttttatatta cagaacagat cgtcccctcg ggttgaatgc tgacgaattg aatcaagaaa
    15541 cgcctattgc aacctatcta tatcaatatg ggtttgcttc tagtcaagaa acaatccaag
    15601 ttttgcagcc atttgagatc gacacaaatg gtcagcaagt ggacttaggt tctcgaatca
    15661 cagggtatca gcaaggtgca gcaggttcaa gttttcttga atggcaagaa ggaaactggc
    15721 gtattcgaat ccgtgggaat aatatagaag gccaagatcc cttactatta gctaaagaaa
    15781 tcgtagctta tttggaagaa aacagtctac cagcacctga acaatttggt aaaattactg
    15841 ttgatatggg tgatacgacg aatcgagccg tagaagtaag ctggcaagaa ccgaaaaatg
    15901 cctatacgat cactcatcaa gatcctatgt ctgcgttgaa gatggcagtt tctatgaaga
    15961 ggttgtgaaa tcagcgcgct gatctgagcc catcatttaa ctccaagtca caggaagtaa
    16021 tcaaaaatag cacgccgcat ttttgcataa ttttggctta ataccttaaa agaaaactag
    16081 aattgcctct tcacacatgc taaaataaaa cagacaaaaa agaaatgggg atataagttg
    16141 actgaatact tgaatgtagg aaaaatcgta aacacacaag gtatcaaagg cgaagtccgt
    16201 gttatctcaa caacggattt cccagaagaa cgttacaaaa aaggaacggt gctaacacta
    16261 ttccaagacg gaaaagcacc tgtggaatta acagtcaaaa gtcatcgcaa acataaaaat
    16321 tttgacttat tgagttttga aggacatcct tctatcaatg atgtggaaaa atatcgggat
    16381 ggcatattaa aagtgtcaaa agacaactct gtagatctag ctgaaaatga attctattac
    16441 catgagatca tcggtctgca tgtattagaa gatggaactg agttaggtaa agtaaaagaa
    16501 atcttgtcac caggtgccaa tgatgtttgg gtcgttcaac gtccgaaaaa agctgatata
    16561 ttgcttccat atatcgcttc tgtcgtcaaa gaagtcgatt tagaagctgg agtggtccgt
    16621 gtagaaattc cagaaggact gattgacgat gaagattgat gtattgactt tatttccaag
    16681 aatgtttgaa ggtcctatgg gggaatccat catcggtaag gctgtagata aaggattatt
    16741 agatatcaat atctctaact tccgcgatta ttcagataac aaacatcaga cagtagatga
    16801 ttatccatac ggcggtggag caggtatgct gctcaaagtc cagcccatct atgataacat
    16861 ccaagccatt gaaaaagctg caccggatgt caaaaaaaga gtaattcttc ttgacccagc
    16921 tggtaaacga ttcgatcaga aaatggcaga agaattttca caagaagaac acttagtatt
    16981 catttgtggt cattatgagg gatatgatga acgtatccgc acattagtga cagatgaagt
    17041 ttccttagga gattatgtcc ttactggggg agaattaggt gcgatggtca tgatcgatgc
    17101 aacagttcgg cttttgccag atgtgttagg gaatcagact tcagctcaaa cggattctta
    17161 ttcaaccggt ttattggaac atccccaata tactcgcccg gcagaatata aagggatgaa
    17221 agtaccagaa gtattgacaa atggaaatca taaattgatt gaagaatggc aattaaaaga
    17281 atcactaaga aggacctatc aacgtcggcc tgatcttttg gaaacacttg aatggacacc
    17341 tcaaatgacg aaatttttag aagaaatcaa gaaagaagaa caagaaaaaa cagcagatga
    17401 agccaagtga ctttttctaa aatcttcttt acagtaggtt gataatatgt tagtatttta
    17461 tggtgagtgc aaaagcactt aactattacg atattccgct gtgagaaaag ctcataagaa
    17521 tatttggaat aaggagaaga aaaccatgaa tccattaatc gaagaattaa caaaagaaca
    17581 attacgttct gacatcccag cgttccgccc tggtgacact gtgcgtgttc acgcaaaagt
    17641 tgttgaaggt actcgtgaac gtatccagtt atttgaaggt gttgttatca aacgccgcgg
    17701 tgctggaatc agcgaaactt atacagtacg taaagtttct aacggagtgg gcgttgaacg
    17761 tacattccca ttgcatacac cacgtgttgc aaaaatcgaa gtagttcgct acggtaaagt
    17821 acgtcgtgca aaattgtact acctacgtgc attacacgga aaagctgctc gtatcaaaga
    17881 aatccgtcgt taatcattgt tatcagactg agaaagaacc cttggtattc aagggttctt
    17941 ttttcttagc atgtaatttg aacaccctga tccagttttt tctttaatta ttggtagggg
    18001 agaataatgg attagaataa atctacctat aagtagtagt aaaaaaaaga gcactatgat
    18061 aatatctttc ttaagagtaa ccgctttaat aaaaaatcca gaatattcta acataaatgg
    18121 agtattctta gaatcaaaag gagaaagaag atggtttata cttatacgga gcaagaaaag
    18181 atgaaagttt tagacacatt catgaatgat ggaaaagtaa aacaagtacc aagttcaaca
    18241 aaaaagaaat acatcctatt gaaagaagtc ttgaaacgct ttgaccatgg agtgacttat
    18301 acagaaacag aggttaattc gatactgctg aacgtatttt ctagtggcga ttatgtcgaa
    18361 caaagacgtt atctaatcac atttggcttt ttcaaaagaa gttcagacgg tcgggcatat
    18421 cagatgatgg gaattgaaaa ttagaagttt taatttatac tcattgaaaa aagttcaagt
    18481 atgcgatatt ggttctacac cgcatacttg aacttttttt gtcatctgta tctataatac
    18541 aaagccaaga acgttattca aaatatggat agctatgata aaagctaatg agcgattcgc
    18601 agttaaataa gcaatcgtca gtaaaatcgt tccaggagcg tacatgaaaa aatcaattgg
    18661 atgcgttaca tgaataaaga taattctctt ttatttgtac gaataaatac caaaacgtac
    18721 ctcctaaaat tagattttgg acagatgccc tttaaaaaga taacaagatt tcgttcttga
    18781 aacaatatat atgcttttat tccaatgagg ttgtgaaaac agtattttgt cctgagcgtt
    18841 agaaaattga aaatggggaa tagcaactcg tactttacca ttttttaagt gaatacaaga
    18901 tcagctaatt gaacaccgtt tattcgtgat gagggacaca acactgcagt cgacacattt
    18961 ctcttttatg ttcaaagata acaaatattt aaaacaaaga aaaatagtag ataatcccgt
    19021 cctaactctt ttattcttcc ctcatagctt ccaaatttaa gcaatacttc aattaatcaa
    19081 gttttatctt tttttttcaa taatcgtaaa aaaagagtat tttttattt
//
LOCUS       DACDYJ010000050        18895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDYJ010000050 DACDYJ010000000
VERSION     DACDYJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.050"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(21..251)
                     /locus_tag="IWM51_001873"
     CDS             complement(21..251)
                     /locus_tag="IWM51_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971290.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="IWM51_001874"
     CDS             complement(687..1253)
                     /locus_tag="IWM51_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9971291.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="IWM51_001875"
     CDS             complement(1253..2209)
                     /locus_tag="IWM51_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP9971292.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="IWM51_001876"
     CDS             complement(2209..2865)
                     /locus_tag="IWM51_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9971293.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="IWM51_001877"
     CDS             3235..5253
                     /locus_tag="IWM51_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9971294.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5680..8094
                     /locus_tag="IWM51_001878"
     CDS             5680..8094
                     /locus_tag="IWM51_001878"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP9971295.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="IWM51_001879"
     CDS             8442..9995
                     /locus_tag="IWM51_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9971296.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="IWM51_001880"
     CDS             complement(10376..12160)
                     /locus_tag="IWM51_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9971297.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="IWM51_001881"
     CDS             complement(12175..13896)
                     /locus_tag="IWM51_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9971298.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="IWM51_001882"
     CDS             13996..14757
                     /locus_tag="IWM51_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9971299.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15724..15992
                     /locus_tag="IWM51_001883"
                     /pseudo
     CDS             15724..15992
                     /locus_tag="IWM51_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16664..>18895
                     /locus_tag="IWM51_001884"
     CDS             16664..>18895
                     /locus_tag="IWM51_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971300.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6204 a         3353 c         3787 g         5551 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata agtatttaga gatttttctt ctaacattct tcaaacaaga
    15301 aaaatcacaa aggttattgt tatttttata tattcttttt aagaatgatt gtttttttat
    15361 agaaatttta aaaaataaaa cacgttattg gtggatagtt agtttgttat ttggtaatat
    15421 tatagaagtt gactggtagt gatttatttt tcgtcgtttt ataaaaatgt tattttttgg
    15481 aaaaattcac aaatatgtaa cgaatgtttt tctggaattt gctgtgttaa aaagcagtat
    15541 gactaatata aatactattg tcatttatgc aattttttat ggatagtaat tttagagggg
    15601 aatcaatgaa ggaagaatag aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata
    15661 gcaatacatc ttcaagcttt aaaattcgag agaatcatct tggtagtgga atcaactaca
    15721 acaaaaggat taaaccaatt aatagataga attaccaatg gaaaaataga aaaggtagtt
    15781 attctataca aaagttgttt aattcgcttc ggttatgaac ttagtgaaac cctttgcaat
    15841 atatggtaca gaatatagaa attattgaca ttacagaaaa tgatgaagat ttaatacaga
    15901 ttgtcactat ttgtagatac cttttacaag gaaaccgagc caataaagct aagaagatga
    15961 ttaaggagtt gatggaagac gatacaagtt aagaaagtga aactacgccc aaataaagag
    16021 caaaaagagt aattggacgt ggaaaatgca agaaatgaac catcgattcg gtggacagtt
    16081 tattagtaat gacggtttga gaacatccat gacgagaatg aaggaacatc ctaaatgcgt
    16141 ttagttgaac caagtgccaa ataatatggc caaataagcg atcagttatc gatgaatccc
    16201 aagtattcta aactatatat tcattttgat gctaagcatt gatatatttt tttgggattg
    16261 atgaaggtta accaatacag ggactcactg atgaaattct agagtctgac cttggtatca
    16321 acgatttagt gattgtttct aatggaaaag tataaaagta tataagtaca ttaacaaaat
    16381 aactattagg gttacgctcc agcttgtgca aggcactggc aggaggaatc gtgaaatcat
    16441 aattatggtt aagccacacg gacaactaaa agtcatacaa agctatcgaa tcaatcaaca
    16501 gaagacaatc ggcaatcatt agctttgttt acagcttaca ttgcaacgct tgagttgaac
    16561 cgaaaatacg agggaggtaa cgcagccatt ttgagttggg gaatcttttt agaaaattat
    16621 cgggtacctt gttgtttagt caaaattata agggggagta atagtgagga acaaatttca
    16681 taaactgtta agtatgtttc tagttatgct tattgtgatc aatcaatttg ggaacataac
    16741 ggttctagct gagtctttag cagaacagcc agagcgttcg ctgacgatcg aagttgatca
    16801 agaggaggct gctttgggtg agcagttcca gttgcgagtc agaggaacac cagacctgct
    16861 aaatgcattg gtcattgata agagtgagga tttgattgaa ggggaagtga agcaactcaa
    16921 tgattacgaa cgtctgatta cttattcttc ccagcattca ggtaaatacg aattgaccgc
    16981 aagaagcggt gatattcaat caaatacggt ctcaatagaa atcagtgtac cagaagaaag
    17041 cacaagtgag tcttttaatg taccagaaga aagcacaagt gaatctttta atataccaga
    17101 agaaagctta agtgaatctt ttaatgaagc cagatcatct gagccatcta taacgcagga
    17161 atctcagatt gaaagacaag aggagctctt gaattatgac atcggcatct ctgctgagcg
    17221 ccccactttg ccgagcaggc atacacaaac gttaaaggga tacattcaag gaggcgctgg
    17281 cgatattctt gaagaatatg gggagatggt cgtacgagtt cctagagcat cgattggctt
    17341 aattgagttc attagagtgg aggtcgctga tgattcacct ttaaagccac tggcatttta
    17401 ccaagaaggc acagacgttg tttttccatt tacctttgat tatgaaggat taaatgaagc
    17461 cgctcagtcg tatacattca gtatcacgtt tgaagttgct ttctttcaat caggcggtac
    17521 tgagccaaat aatgactttc aagtgagtct tacttatgct gagcgagcag caaacacgga
    17581 attttcaatt acgcctattc cttcatcaag acccattttt tcgaaattat actatggctc
    17641 tagagaaatc gacagccaag gaaatcttgg gaatctgctg attgacgggc aagacgtgac
    17701 caacagtaat aaatttattt tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt
    17761 ggttaccgat gtactaccgc aaggaacaac tttggcaccg aatccgacta catggagttc
    17821 cactggaaat agagaaccta tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg
    17881 tgaagcaaac gttagaactc attatgtttc cgaggaattt gtggacaaag tatcttttaa
    17941 taaggaaacg aatacacttg ttgtggactt tggtaatcta gcagagggtc aatcgtattt
    18001 tattgaatat gctcttgaag tgaccgatgt cgacttgttc aatgaaggaa gacaagagga
    18061 tgggttttct cggaatcgag cgagtatgac ctacaataat caggtaatga gccgagacta
    18121 ccgcttgaga atccctgaaa gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa
    18181 aaatgttatc aatctcaatg aaaatgaact aacgtatact ctgcatgtct cgatgttttc
    18241 tggaacaaga aatacgcttg ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc
    18301 atttctatca gatattccct ctttcattga tgccgaagtc aataatggaa tagttcgttt
    18361 cagtgcgaat aatacgattg gtggtccgac aagttttacc ctctctttta aggtcaatgt
    18421 agaggcgtat gcggtcggcg atcaaatttt aaactttgca acgcttgtga atagtgacaa
    18481 tcgattgaat acgagtactg taagaacaag gaaaatcgat gggcgtatta aagtaattaa
    18541 aactgatgag aatggccaaa ggctggcagg agcaaaattt gaaattttat caagcgattc
    18601 acaagtgata caagaaggta taacggcaga aaacggagaa tttttaagtg aaccgcttcc
    18661 tgtaggtagc tatcaagtcc gagaaacaga agcaccggaa gggtatcttt tagatgcgac
    18721 ggtacatgat gtttttataa atgaatactc catagaagaa gtgacgttaa ccattgaaaa
    18781 catccttgat acgggtggtg ttgaactaat taaacatgct gaaggcagtg gtgaagtcct
    18841 tcaaggagct gtttttgagt tacagaaccg agaaggagaa acgctacaaa ccggt
//
LOCUS       DACDYJ010000051        18708 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDYJ010000051 DACDYJ010000000
VERSION     DACDYJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.051"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(93..1004)
                     /locus_tag="IWM51_001885"
     CDS             complement(93..1004)
                     /locus_tag="IWM51_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9971301.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1381..2019)
                     /locus_tag="IWM51_001886"
     CDS             complement(1381..2019)
                     /locus_tag="IWM51_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9971302.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2023..2793)
                     /locus_tag="IWM51_001887"
     CDS             complement(2023..2793)
                     /locus_tag="IWM51_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP9971303.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSTYWERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            complement(3211..3627)
                     /locus_tag="IWM51_001888"
     CDS             complement(3211..3627)
                     /locus_tag="IWM51_001888"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971304.1"
                     /translation="MMTREKEAYERFILLFVERFNQAFHTDYATNIFEDFENSYSYSV
                     ISSTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNGFSLHQVLWCLLGKKKI
                     IITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGI"
     gene            complement(3678..4059)
                     /locus_tag="IWM51_001889"
                     /pseudo
     CDS             complement(3678..4059)
                     /locus_tag="IWM51_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4185..4850
                     /locus_tag="IWM51_001890"
     CDS             4185..4850
                     /locus_tag="IWM51_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9971305.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5247..7280)
                     /gene="brxL"
                     /locus_tag="IWM51_001891"
     CDS             complement(5247..7280)
                     /gene="brxL"
                     /locus_tag="IWM51_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HAP9971306.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7398..9920)
                     /gene="pglZ"
                     /locus_tag="IWM51_001892"
     CDS             complement(7398..9920)
                     /gene="pglZ"
                     /locus_tag="IWM51_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HAP9971307.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(10036..13668)
                     /gene="pglX"
                     /locus_tag="IWM51_001893"
     CDS             complement(10036..13668)
                     /gene="pglX"
                     /locus_tag="IWM51_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HAP9971308.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSDFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(13732..17403)
                     /gene="brxC"
                     /locus_tag="IWM51_001894"
     CDS             complement(13732..17403)
                     /gene="brxC"
                     /locus_tag="IWM51_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HAP9971309.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(17428..18003)
                     /locus_tag="IWM51_001895"
     CDS             complement(17428..18003)
                     /locus_tag="IWM51_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HAP9971310.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(18003..18602)
                     /locus_tag="IWM51_001896"
     CDS             complement(18003..18602)
                     /locus_tag="IWM51_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HAP9971311.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         5769 a         3566 c         2618 g         6755 t
ORIGIN      
        1 ataattaaac tactgttgat ttagttctaa taaaaagatt tagatgaaca gttttattga
       61 tttaaatagt tcatattttt tatttatacc gcttatttac tacttgctgt actagaatct
      121 gaagtgaatg tactcaaaac attttcaaag tctttgtctt tgatttttac attagcgtct
      181 ttcaatactt cgccaattac tttattctgg aaggtactgt cacttaattt tgtgtctgtt
      241 gcaatttctt tcaattcttt tttgtattta tccatgtcgt tgcctttgtt ttggtttttc
      301 accattttaa ccacataata ttccgtagta taagttgaag cattggtaga agtgatcaca
      361 tcagaaactt gaccatcttt caacttaaag gctgcttctt tgacttctgc cggaaccgtt
      421 gtagtagtgg aatcaaattt cacttttccg ccatcttctt ttgtcgtagt atcagttgac
      481 ttgtctttgg ctaatttaga gaaatcgtct cccttatcag cagctttctt cacatctttt
      541 gcatcatctt cacttgctac ttggataatt tgagcttcca cttctggatg gaaagattcc
      601 caagccgttt ttaaatcttc atcggtcaat ttgatatgtt tctttagacc ttcttgaaag
      661 gctagacttt gtttgatact gtctttgaat gtttgttctg tgtaacctgc agatttcaat
      721 tgtgaatcaa atgaatcacc caattgtttc tttgtttgat catattggga atcgatttct
      781 ttggttgata cgtcatctcc atatttatct tcaaatactt gataaacgat caactgttgc
      841 acagcggaat ctgctccttg catttttttc aagctatcca ttacttcttg ttcgctgatt
      901 gtaccgcctt tataactaac ggctgttttt tcaccggcat tcgtacagcc tgacaataag
      961 ccagtgccta gtacgatggc aagtgctaaa aatctttttt tcatttatca attctcctac
     1021 ttttcgtttt ttgcgaattg ctaaaaacaa aggtgttcaa gatgaaaatc atcaagaaca
     1081 cctttactaa tctgctcgat acgtcctgaa tagatataag gctacacaat ccagaaagtt
     1141 cgctcagcag aataaatgtc aagtagcaca tcacacctta atatactact tcttttgtta
     1201 ccgtttttca agtctatctt gcgttccgta tttaacgtaa ctaattctta gaacaaatgt
     1261 tatcgatttt gtttttcttc ttccgctgtt gggatattca aatcagacac ttttatgttc
     1321 gattttccag acattttttt gttgttttta ttagaaaaag tttgtttttt catttgtttt
     1381 ctaaagcatg atcttcttgt ttcctatttt ggtcagatat ggatcatgcg tgaccacgat
     1441 aactaacgta ccttgggaag cctgttgcga taagatttca acgacttgct ccttatttcg
     1501 tttatctaat gatccagttg gttcatcggc taaaatgatt ttgctattct tcaataagac
     1561 tctcgcacag gcgattcttt gcttctcacc accagataat gtgtagatag gcgtacttag
     1621 cttcttgttt atatttactt ttttcaatgc ctcatacttt gctgttcgct tatctgaaac
     1681 atttaaattc gaaaacttca atccaatatc taaattaaaa tcgattgttt catattcaat
     1741 caacccaaaa tcttgaaaaa gaaaagagag ggcatttctt tttatcaagc gcttctctct
     1801 tccagttaaa tgttttgttt cttgattaaa taaataaaat tgccctgaac tgaattgatc
     1861 attcaatcct aatatattca ataaagtact tttccctgct ccgctctctc caactacagt
     1921 cacaaaatcc ccagcatccg cagtgaaacg atccaaatga agaatcgttt tgtttcgaat
     1981 gatttttttc gcacctttta tcactatcaa cggctccaat tgttaacctc cattcatcag
     2041 caataactga cttttctttt ctgtagacaa aataaaaaca actgaaagaa taaaatcaat
     2101 gaagctaaag aaaaaaatat taaaaagata tttaggctct ctttctataa gaaaaaggaa
     2161 gattacactt gtcgttcccc atttgatgag gttcatcatg aagaaatctt tatacgtttc
     2221 aaagagcgaa taaccacttg aacgaaagat agcgattttc cgacaattgt agacaaaata
     2281 tatgcataac atgacatagc tcaacaagaa gaaagaaaag aaagtgagta aaaatatctg
     2341 tatcaagtaa tgaaatgatc tgcttaacac tttgacagct atctcttcag tctgagtata
     2401 cggaaatgat aactgcaagt tatcacgtaa ctgactttct tcaatcagct caaataacct
     2461 ttctttcgac aaagagccta tcttgatctt taacggtgga tagatttctc cgctgagaat
     2521 atttctttcc caatacgtac tgtttttcaa tgtcagtatt tcaacaaccg gataatgttc
     2581 gatccagggg atagaaggaa taaatgtata aatgggttga ttgtttttta taaatagaaa
     2641 tttcggttct ttttcactga tttcttgata atattctgtt atttgcgcca ggtcattttt
     2701 taaatctgta tgtctatatc gttcagggat caatataaca gggtgcgttt ctttttcggt
     2761 tatttggatc aaccttccag agacatcaat caaaggaaat ttcttaagat aattcagatt
     2821 tatttttatc cctctgccaa actgttcatt ttcttcattc aagtaatctg atacattgac
     2881 taatatactt ccgtttttat tcaaagtttc atataattcc tgatcggctg ctgatgcaaa
     2941 acgattggaa taaataaaat ccacgtggtt tttcccgatg gttactggat aaaatacatg
     3001 atagtcctca ccagcgattt cttttggtgt ttcatgggaa acagctaact gtgaagaaga
     3061 aaaaacgata gttgctaatt cgacattggc aacggtcaaa agaagagaaa acaacaatcc
     3121 aaatgtaaaa agaatgtatt ggatatagtt gaacgcttct cttaacgaag cgattttttc
     3181 aactaataaa agagaaatca aagagatacc ctatattccg ctgaacaaga aaacgtacat
     3241 ctggaacaaa taaagaaatt gctccttata atacataaaa ataaaaccac caatgattcc
     3301 tgcaattatg aatgtaataa tgattttctt tttccccaat aaacaccaca atacttgatg
     3361 taatgaaaag ccattattca acaataaact gatttcttta ttcatcaata agaaaaagga
     3421 tgataacaaa aggaataaaa gaatcaatcc aatagttaaa tacactcgat aattatatac
     3481 gtctgtactt gaaataaccg aatacgaata gctattttca aagtcttcaa aaatattagt
     3541 ggcataatca gtgtgaaatg cttgattgaa tcgttccaca aatagtaaaa tgaaccgttc
     3601 ataggcttct ttttctctcg tcatcaagaa aaattcacct tcaaaatctt ttattttgat
     3661 cgattcttca atggacgtta cgcgaaagga ggtattagaa tagggtacct tctcgtgaga
     3721 agatttgaca tccccttgat aatacaatgt catttcaagc tttggctgta tcttgaagtt
     3781 ccaagatgta tctacttcat tggattcatt tactgttttt ttcatataag aaagctcagt
     3841 ttctgatgag acattttcaa ataccatgat tagctgctca tattcttctt gcccaataaa
     3901 atgttgtggc aatcgaatcg gagtataagt atctgtttga tggaaaaatt catttgtccg
     3961 atagagtacc tgtttattta taataaccaa ggcaaaacca ctcatgatac cggtcactat
     4021 gattgtgagt aaaactgtga actttttttt gattatcacc actctccctt acttaaatga
     4081 ttctttgata aaatcatacc gtttttcatc aaaaaaacgt atctatcaaa tgtataggtc
     4141 atgtagaata aatctatata ctgtagaatc gaaaggagaa gaagatgaac atcggagaaa
     4201 aaatacgaac ttaccgcaga aattgtaagc ttacgcaaaa agaattaggc gaaaaaatcg
     4261 gtgtttccga taaaacaatc tctagttggg aaaattctcg tacaatgcca gaccttgaaa
     4321 tgctgtcact tttacatcag actttaggtc tgccgattga ttttccttct attgaatcag
     4381 ctaccgaatc aatagaacca acattgccgg atgaacctct tgttatcaag aaatcttatt
     4441 catcaaaaca acccttctca ctcaaaaaga cactatcttt ctttctgctc gtcttttgtt
     4501 tggtctatct ttttcttatc aattatcaat ataaaaatga aacggttgat cgattcaatc
     4561 cttttttgaa accacaaatc ggctatacca cactccctgt aggatacgtg gaaaattcca
     4621 tcgaacattg ggtatgtaat gatatctttg ttcatggtac atggctgtct tttcacgctg
     4681 gagaggtaga aaaaggtaag aatcatgctg taatcaaaca caaaggaaaa cacgtcatga
     4741 acgttcgcct gctcacttgg gaagccattc cctcagatta ccaagaaatc ctcatccaag
     4801 actatgacga tgccttaaaa agaagccctc aggagccaga taggcattaa gtaggagatg
     4861 aacgtagaga tagaaaaaat caatgcacat gatcttatca aaaaaaaaca ggggtacaaa
     4921 ttaattgaaa aagctttgag atgaaagttc ctaaataaaa aagttcagaa tgataggtaa
     4981 aaaaatgact aactattcta gtattaggct atttgtatta ttcttctgtt atttttatat
     5041 caaaaaatcc agaaataact aaatataggt atttctggct tatagtataa tatttacgcc
     5101 acagccccca atgattcttt ttggaatcat gatcaaggac ggacatacac aaaaatattt
     5161 tgcccattcg atccgaatag attaacgact aatctagtaa gtaatataga ttggtacgta
     5221 gtcaaaccag caaatggaaa agttgtctat tccaccccaa gggctttaaa gacagcatct
     5281 tccggcgttt gataaaaaat caaattaaag gtacccatta attctggggg tacagttcca
     5341 atatcagcag cgcttatcat aggtacaagt acttttttcg ctccggcatc ttttgctact
     5401 tgtaaacagt tagctaaatt atctacctta atcatagaac caccaattgt aatatcccct
     5461 aaaataacta aacttggtaa tacaggttta tttaaggaaa ctgagcataa agctattata
     5521 gttgataacg ataatgtttt tgtcatacca atgccattca tatcttgaac atgaacgaca
     5581 aaatcttttg tcgtcaaact aatagactga ctaacacttc cataattagc tcttaaatat
     5641 cggtatgccg aatccatcgc ttctcttgct ccagcatctg aattgatccc agtacgttca
     5701 aactttccat ttcccgaagt catttgcgtt tccaatttat aggtacctaa ttgcccagtt
     5761 ttgccataag ataccgtgta caaatgccct ggcttcccta acccttctgg aatcaaagtt
     5821 cctccacctt gttcaggaac agaaacatac tcttctcgct cttcttctaa atcaatgtat
     5881 gaaaaattga catcataaaa ttccattcca ccaattttct ttaattgttc ttttacacga
     5941 cgacgcattt caagagcaaa tcttagtatt tcttcgacat cacttttttc aaatctacca
     6001 tcaggataca acagtttcat catcccagat acaatccgac gaactgcaat cgtatcccgc
     6061 tgatttaatt gagaaccaag tttgaaatat ttatcaaaac tatcgccaaa actaactttt
     6121 cgaagctcac gcatgacttc agaaaaataa tctgtaatga agccatagtc atttgtaaaa
     6181 aaatctggtc gatatttagg aatttcccaa cctggtaaat aacaatgcat ccgatccaaa
     6241 aaggctgaat ccgtccccat aacctccgga aatggctcaa ataaattcga agttttcaat
     6301 attacatcaa cgctttgatt aatatttcct acaaaagcca ttgatgctga agcattcttt
     6361 tcttctttgc ctctagcaaa agatcctgaa gccatgtaat ctttcatgat ttgaacgcca
     6421 tcattatcct taaatttaat cccagcaact tcgtcaaaag caacacagtc ccacatacct
     6481 acaagtccca ttgtacgact agccatatta tagaaaaggt ttgctactgt tgtttgtcca
     6541 cctgaaacaa ggattgagtt tggtgaaatt tctttatata catgcgattt acctgtacca
     6601 cgtggtccta gttcacagag attaaaatta ttttcaatta atggaataag tcttgctaaa
     6661 tgaagccatt ttaccctttt attaaattgt gtaggttcca taccaatgga ccgtagaagt
     6721 aagtcaatcc attcatttgt agtaaactcc ttgcgagcca tcttgtaagc ctcaatgtct
     6781 aaaccaggca tttggattgg atcaaccttt tttgcaataa aagggctatt ccaacgatca
     6841 ttctcatcat accaatattc catcgaaaca atacaccaga tccccccacc taataatcga
     6901 tcaaattttt tcggatactc ttcactaatt tgaacatttt ttaatcccag gttagagaat
     6961 tctgcttcat aacaatcttt cttttcattt aaatgtacag ttactttatc aataactgta
     7021 taaccaccca attcacgaat ttttgctttt actttttctg cttcatcagg acgcacatag
     7081 tttcgagcaa gaatattttt aacattttcc acaccttttt caaccaatgt ttcatccaat
     7141 gacgaagcat acataccaag taaatattcc aatacataaa caggtacatt cgcaccttct
     7201 ttaattttct ttgttaaatc ttttctgaca atctttccgg gaaagaattc agataacttt
     7261 aaaatttcct gttgttccac tagaattcct ccatgtgttc agtcattata atagcaatta
     7321 aagtatatca tacttaacca gtaggcttga taatacaaaa gaaaaagtat ccacaaaaaa
     7381 ttgtggatac ctcgttttta tatatcaaaa tcaaatgatt ctgcaaaagg attatctata
     7441 atgaatttta ttcgctcggt caaatcattt ttttgatttt ttatcactaa ataataagta
     7501 tcttcaactg tatatcgacg atttacaaat tcaaaaacaa atttagtaaa ccgctgattt
     7561 gcatcttctg tttttgaatc cgcaacatag atttcttcat ttgagatacg ttcaccatat
     7621 tcatcttcaa aataaagcca gaaagatgcg gggtcataaa catcactaat tgcatttttt
     7681 tgatagaatt ctaacgtggt tgataatcct gtaattctgc ggttgctagt cattaattca
     7741 attgctactg gttcctttgc attgctacct ttggctaccg taatttctag aacagggact
     7801 agcatttctt gaatagatga ccctccatga acgtagcttt gtcccccgcc agctttctta
     7861 aaaatacttg aagtaatcgg gacacaaatg tttctagtgt catcatttcc taaagaatct
     7921 cccaaagagt aatttttcgt accaattatt tcgtattctt cttcactaag aataaaacga
     7981 cgttctacac gatctttttc atttctagat ggattttcaa tcttatctac ttcttcaacg
     8041 tggcttcttt tataaataaa tccatgatca gccgtaacta taaaacgata gatactgcca
     8101 tcattatgca agcgattaat aagtcgggaa atttcgctta tcgctaaatc acaggcctca
     8161 aaaactcgat tttcattcga gtgatcacct gttctatcca cttgatcgtg ataaatataa
     8221 atgactgatt tgccattaaa tacttctcgt acctcagcct tattcatctt aaccacatca
     8281 tcataattta aagctacagc atttgcatct cgttgctgga gaatcgcatt tcgattggat
     8341 aaaccattcg tttttttccc atcaatcagt acttctgttc cttcattgta agttagttga
     8401 ttgtttggta ataaggcggc tttaccgaat tcagtaactg aaggtaatac agaaagaaag
     8461 ctagccatgt ttgttgaata cttcttttct ctaactaact tacgttctaa attcatccct
     8521 aactcatatc gaaaagcatc tgaaataata actacagttt taacagagcg tttcattaca
     8581 gcttgattat aaaattttcg aatggatgga cgattcttaa attcaaaagt ttgattccaa
     8641 atttgtcctg attgatcaag aaacttcgaa taatttgctt caactaatcg atacaattgg
     8701 ccatattttt gcttttcttc tatatgatca ctattccaaa taaatttccg ataatgcgta
     8761 tcaacccaat acatttcctt ctcatattga gttagcaaat tatccatgtt catcggcaag
     8821 cacaatctgt ttttatccac aagataataa gcatgtttca ataattgata ctcataaata
     8881 taatccgatg cgaaatgcat tttttcgcgc tgctttaaac tatcaaaaat tgtttgtcct
     8941 gcaattttcg ttgtagtgtc agaagccttt aatcgttctg cataaaacat taatattttt
     9001 gcatgaatct cagaaaatac gtctgcttca attagatcat caatttctat actgtctaat
     9061 aatccatcac catcaatagc ctgatatact tttttagata gctgttcaaa gctttccatg
     9121 taacgtgtat cattcataaa ctgatccatg aaagtaataa tgtttgcttt caaatgtagt
     9181 atttcaaatc ctttgaactt cttcggcatc tcttgatcaa tttgataata aaaatagttc
     9241 aaataaagac acatcgtcag cttttccaat gttggctggc tatccgtaaa gccaaatttc
     9301 atttgaataa attcccaaaa tttagctgtt aactgatact tatcaagttc ccttaaagga
     9361 ttctccctac tttctgtaaa gctttttaga actacacgca gtaaatcatt taatcgaata
     9421 cttttcgatt tcaacaaaac agccattatc attaattcaa tcgtttctga tttattaatt
     9481 gtcatatgcg tactaaattc accaaaactt ctcatgcgat cctttgcatt aaagaaatta
     9541 cgatactgtt taactgtctg atgaaatcta agtggaatag ccaattcatt catgataatc
     9601 gctaagcggt ctgcactaaa ttcctctgaa taattcaata cactcaacaa atgattatct
     9661 tcatctttat catcccaacg aggaaatggc gcatagatta aatagttagt ttcgggatct
     9721 tcatattcca ataaacgctt agttttaaat tgtgcgtcct tttctaatcg atagattttt
     9781 gcattttgaa tgaccaatga gtcaatttct tcaagaaaat cctgatttga atcaaaccaa
     9841 aaaatgattt tcctacatcc ataattagga aaacgttgat ttaatttttc ttcaatttgt
     9901 tgcgtattta tatctgtcat caatctcacc taatcgttct ctttctatac cccttatatt
     9961 gacataattt ggataatttc gcacgcaatt taagaataaa aaagaaagtt gttagaaaac
    10021 aactttctta gataactatt tttttggttc tttgacccct tttgcaaaaa tttggaattt
    10081 ctcgccggtt ttaggatcac gctgcaactt atcataattg acaattacac catcatctaa
    10141 gtctaattca attcgtttta atgctaagtg acccaacttc tcgtcatact cacgaacttc
    10201 ttttaattgc ttctgaatct ttacaatttc ttttgtcatt gcatttcttt cttttgggtt
    10261 atcacttgtt gaaatttgtt gctgacgcaa ttcgattcga ttgtcatacg ctttttgtag
    10321 ttcgtgtagg tattcagtac gtacttttcc taattgatct ggtgtatagc gatgcaagta
    10381 cattaacgct ttaaagccat tttgttttcc tgagtccaat tgccaataaa ttggtcgatt
    10441 ggagtatgtt tttaaatggt ctttgtaaaa atcttgtacg aagtaacgac gaataatttc
    10501 tcgacttgtc cctttgccac ctaaagtgtc agcgataaac tggagatttt cttcaagggt
    10561 ttctttacca aaagtaatct caacgaaatc aatgaaacga ttgacaatgt caatttggct
    10621 atccttgaaa tactcatcat cggtgatgag taaaacatta tcagtatcag gtttaaattt
    10681 ttgatagcgg gaaggatccc attcgccgcc agcgaagatg agaccatctt cgtctaggga
    10741 gtaacgaccg aagatacaac ctattgaata agaaataaaa tccttaattt ccctcgcctt
    10801 atcaggtaac cttgcagaga tatattcatc ttgaacctct tttgttaatt ctttttcaag
    10861 agaataatga ttaatgaata tgtcgttcag ttcctcttca ttcgatttta gagtattaaa
    10921 ttgattaacc aactgcctac ttagacagtt aaatgcctct gagagatctt ttttaaacat
    10981 ataacttaac aaaatgtttc ttctaaactc gtatgacgtt tcaagagcat tatagtcttt
    11041 tctagctata tcgatatttt catccacttt ttctcttatt ttttttaatt ttctctcaga
    11101 tgtaacttta agaaaaggta gttttcccat tggtccttca tggtaatcca aggtgggaga
    11161 aataaaagtt aaaaagtgat taactaattt agagtttaca tagccaaata tttcttcaaa
    11221 gtcagttata tcctttgaga acagaacaga tcctttagtt tcaaaaattg cctgattctg
    11281 caatctaaaa gatacttgtc cagatgaaag tgtcgaccaa gtaattccct ttttaaaata
    11341 gtaattaaga ttttgtggtc tagattttaa cttcccattt tcattaacat aattttttat
    11401 ttcatctcca tcattctccc agttaaccac gtattcattg tttccatacc attttctaaa
    11461 acgaccacct ttagttgtag gaaaccattt ttttctagac tttttagcgt cctctaatgt
    11521 tttagcatca aactttattt tgttaaattc tacttcaaac caataccgca aaaatttatt
    11581 gttatttccg gttgccaacc cttgttttgc atctgcaaca tcgttagtta attttccttg
    11641 agcaaaatca gtgattacgt tatcgcttgc ccagtaagca atcggcattc ctgggatttt
    11701 tttgaagttg tcttgattag tactataaaa ataatcgcat tcaccatgct gatgaattgc
    11761 ttctaacgtt ttaatttttt gaacttccat tccaccttta aaatctgata attttatata
    11821 gcttccatct tgttcatttg aattttttat cgtaaacgta caaattggaa cggttgcttc
    11881 ttcaaaatct gaatactcca tttgaatcaa actagaaatg tttctttcat taattaaaaa
    11941 tgagcgtaac ttttcatagc tagaaatgaa catccaaaca aatggcgtca taaaggaagc
    12001 atatccatta tcaatcagca tttggctatt caatttaata aacacagaaa atagatcttt
    12061 tttgacatca gaatagttat catttacata tttttttaat ttcttatcca ttttattaag
    12121 ataaggcgga ttagtgacta ccgctacata tttatcaact agcatttcag ccacgtcaag
    12181 aatgtgcaag tatttttctt gtatttcctt catcccataa agaatatcca tattcgtaaa
    12241 ttcgttgaat tttgatatat actctcttac ttttcctaag tattctttag ttattgcctg
    12301 attattttta tcgactaact taatgattga tccaagttct ttcccatttt caaattgatc
    12361 aaaaattaac tctttgtatt cattccagcg ttcactatac ttatcattgt tcaattcact
    12421 aatacgctct tgatactcgt ctgtcacttt aggagaatca ataaactctc ttaaattcgg
    12481 cgatagactt tttttcaaaa aaagacgatc gtattgacgc cctttcatca taattgcaaa
    12541 ataagacaat tgatacgctc ttttatcgat ttcaagacca tataaattgt attcaacgat
    12601 tgcacgtgca gcatcacgct cagaatatcc taacgtaaga taaatttcca tcaatacatc
    12661 gaaggcgtaa actaaaatat gcccagaccc cattgcgtta tcgataattt ttagttcttc
    12721 tggttttaaa ttacttacag atgttgcttg ttcaaaagca tatttatctg gaattaagta
    12781 tttcaactta tttcttaatt cactattggg ataatgatct aaccaatact ttcctaaact
    12841 attgtcaacc atataacgaa cgacccaatc tgtggtaaat agttgagttg ctgccggaat
    12901 atcttgtttt ttgacaatcc ctttataaat attcaccact tgattatgtg gctcactatt
    12961 atagtactga tacaaccacc caataatctc tacctgtcca ccagcttgaa catagaaatc
    13021 atcctctgga atacttaaaa gatcagcgat tacaccatct tttttaatat aagaagctgt
    13081 aaacaaaagt tctgtataat cttctacttc ttcaaatagc tcaggtaagt aatcattcaa
    13141 ttgattacag atttttataa aaatcaattg atacagcttg tccattgctt ctgctgaatt
    13201 atctgttttc caagttctga cttgttgctg ttcattacta gataattctt caaagatatc
    13261 ggcatcataa atatctgtaa taatatctgg ctcaattttc ccacttattt cactagataa
    13321 tacacgatac gtgtcaggta aataatcatt tacctccata aaccgaatgg caatcaatcg
    13381 gttaaaccac gtataagcaa cctcttcgat taactcttga aaagcttctg aataagaagt
    13441 gacagaagca cgttgtttta gttctgcaat caatttgttt ctttgcgcta tttcttttcc
    13501 ttgaattctt attcctgatt tttcgatttc ttgtaaattg ctatcaattt tcttcacatc
    13561 ttcaattttt gattcatcaa ttcctaaacg ttttgcttta gtcattactt catcaattaa
    13621 ttttctacgg gcattgaccg cgaaattctt tatcgctttc ttatccattt ttctttccct
    13681 tctatatgta acctttgaaa agacaggcac ctttttatgc ctgtcttttc actagaattg
    13741 aatcgtgatt tctgtatttt cttgaagttg ttccttcagt ttttgattaa tttcagctgt
    13801 taactcatca atatctgctg ctgacttaat agttttaaca tgaacgttaa gtgttctcat
    13861 tggtactgtt ttctgttgaa tagtaggttt tactatcgct atttcttcag cttttgctgc
    13921 agagtctatt tcatcttttt cagtttcacg aatcaagttt gctgcttcaa tttgtttacg
    13981 tgcttgttct tcttgacgtc tgcggttttc ttcattatcg aaacgttgat taaagatcaa
    14041 atactgttct gctacgttta atgataagaa aacaagatca tctatagtat tcgcttgttc
    14101 aatttttgct ttcagttctt gaaattcttg gtcaactatt ggtacaaatt gatccttaaa
    14161 atcttttcct tctaataatt taaatgttgc ttgtttttct tccgttaatt tatttaatat
    14221 ttcctgactt tccaactcta acagttgatt atattgttca ataaattctt gaatcaactc
    14281 atttagtttc atcatatctg aataaggatc agcctttttc aaaatggttt tcatctcatg
    14341 aatcaattga tcaatttctt ggctagaagt ttgacgccga cttttttcga ttaatctaat
    14401 tttatcttgt gcattctccc agattttttt ctgagtacta ttttgcttgt ggaaattttt
    14461 aattttatca tatgtttcag cccagtcgaa taattcatcg cgatattttt tgattgtata
    14521 aaaaaagtct tcaacatctg acgtttgaac aattacttca agatattttt ttatttgctc
    14581 atattcataa gcactagggt aggtataagt gttttgtaaa tcaaaataag agcgtaaatt
    14641 gtctaattga ccttttaaat atttcttgaa attttccatt gtatgttcat tgttattttc
    14701 atcaacaata ttacgacttt gaaacagttc tctaccgatt tcgtcaacca atttttttgc
    14761 ttttggcgaa atcaccgcgc ttggttcaat ttttatcttt tcactttctc gtcgtttttg
    14821 aataatcatt acaatactat ttggatcatc catccgtgaa atttgtacat tgttataaag
    14881 taaatcaatt ttaccagaca tgaaaagttt agcgatgcac cattccacat caagttctgt
    14941 gaatccatac ggttgttttg tttcaaaacg atcacgaatt gttttcatcg taatcgttag
    15001 gtgatttctc ttttgatcat tgataaaatt gaaaatttcc tgaactgctt gctcatttga
    15061 tggatcttca ataatttcat ttttgctttc tttcactaaa tataagatat cttctttttc
    15121 tttaggcgca tcaatttggt ttaatttatg gtatacttct tcagccattt tctgaagtgc
    15181 tgattggaat tttgctttaa agtcggagcc tgtcagttga atttctgcac cgttatagaa
    15241 aaattttgca gatttcaaca gttcatttaa ttgatcgttg atacttgcac ttagttcctg
    15301 taattcacca gatttttccg aacgaatcgc gtcaaagttc tttactacac tcgaataagt
    15361 cgttgttaaa taaagattca ttctagcata cattgctata tcatctttaa attctttatc
    15421 tgatggcaat tctacgaata ataagttacg ggaactctta agcgcaagtt ctgcctcttg
    15481 acctgctaaa tcagagtatg gtgaaataaa attaactgtc atttcagcat ttccagcttg
    15541 cttgtatgga cgttgatcta acacttgtgc ataagtaaac gtataccggt ttttaaattt
    15601 atttccacct gacatgccta atttttcata ggcaaaacta tttcggtcta gaacgccgtc
    15661 aaaaattcgt ttagatagtt ctacgatcaa atcactatct gtaatatttt gatttttaat
    15721 ctcacgagta atttcctgtt cttcatcagt ttggaaaata taactatcta agttcttccg
    15781 cacaaagttt tccttaatta aaaggtccaa tgcttcttct actcgttttt tagtttccag
    15841 gcggtcttcg ttgatatttc ttatcatcaa gctcgtaata ttttcaacat ttggtttgat
    15901 ggcttgatca acatatttaa tcatgaataa aattcgtaag acatttacgt taaagttatc
    15961 tatatcagtt ggtctttgaa tatgagaatt atttgtagct tgtgtaatca ctcggctaat
    16021 tgctgtatca atccaacgtt ctaacgagta ataaaactga tctaaagaaa ccaaactggt
    16081 tgtatcattt tccttatttc gttcagcggc ttctttaaac attgctagta atgaacgttc
    16141 accagacgat aagtttgatc cttgatagct gtgattacga atctcggtta gaatttgccc
    16201 caataaattg aattgatagg gaataaatgg ataaacttct gcgtaatttt cagcatcttg
    16261 gaagagcttt aattcaggac tatcaacaaa gagtaattta tttttcaatt ctgtttgaac
    16321 agtttcaaat tgtaaggcta aagaatcatt ggcaaactct tttttcttca aaatacgttc
    16381 tttaattact tcatcggcat ttgctgaaga taaactcaca gttgtggcaa aacgatcctt
    16441 gatcttagaa aaatcattat cagaattgtt atcaaagttt gttacgtgat caatcgcttc
    16501 ctgacttgtc actaataccc aagccttacc ttcacaaatt cgacccaaat cttccacaac
    16561 ggtttgtaac tgcaaagtca actcacgatt accgccgata tattgtccga cctcatcgac
    16621 tagaaaggca atatgatggt ctgcttcttt ggaatcaaga tagcttttaa ctagatttgc
    16681 aaagtcatca atgcttatta aataatttcc tgatgctttt tctaaaaaat tatcaatagc
    16741 ggcttcactc ataaaatcga ttttaagtgc cgcttctttg atatcatctt ggatccaatc
    16801 aaactcatgg cgctcttctt gccattttgc cccattaatc tcttcaaaag cagctttgaa
    16861 tgcatcaaac ttttcttctt taattaaccg acgttcaaaa tctgctagat gtggaatcgt
    16921 gcttaatcct tgcatttcgt taaatacctt taaaaaaaca ttcacgattc cgtttttgtc
    16981 ttttttacca cctgcatcac tttttgaatc aatattaaac agaataacat ctgtatttat
    17041 ttgtgagcac cgttccatcg ttgccaaagt cagtggatct ttgattttcc catcttcttt
    17101 gaagtaatca atggctttct tgtcatttat ttctcgattt gcgagcaagt aagaaataat
    17161 ttttaagaaa tgagatttac ctgaaccaaa gaacccctga atccaaacac ccattttatc
    17221 tgttggtgta ttgatactcc gttggtaggc atcaaaaatt gttttaaagt gtttttgtag
    17281 ttctttcgta accacgtatt cttctaattc ttgttgaaca ttttcatcct ttgcttgacc
    17341 gaccttaata actccttgaa tatttcggtc aattggtttt gcaaaaatat ctttaattag
    17401 catttaatct cctaccttcc atcccaacta aattctaaat gctcgataat aattatcatc
    17461 tttattggta ttaagaattg tcaaacttaa ttcatcatat tcccctggat acatcatcac
    17521 aactggtacg cgatcaaata cctgatgcat tttatttaaa atagagtgcg ctctaattaa
    17581 tggatataat tcgccaattc ctgtcaggaa cacaactgat ttttctggtg tatgggcatc
    17641 cacatattca aaaaatacat tttcttcctc aggtaaatcc attttcaata aacgaccaat
    17701 gctttccact aatttttgta aaccacgtcg ttcttccatc ttaatcgctt tttcagtgaa
    17761 accgcgtttt tctagatact ctaacataat cacatagaga ttataaactt tcacttcata
    17821 attatcacca acagatgttc cagatatatc attaatataa tctcttacaa ttaactcgtc
    17881 tttcggatcg tagtcaaaga tataatagcc tacttcatta cctaaaccac gattcaataa
    17941 aaaatcttct ttttgaatta cttcattcaa cttttccaaa tgctcttgaa tcgataatga
    18001 cattataaaa ttcctcccag tgctagtaca atatctttac gattcttgtc aactaaccat
    18061 tgaatcaatc gtttatctaa taatatcgga gtcattcgtc catattcttt ttcccctctg
    18121 actaatcctg tttcaattag attcgtttga taagcggcaa taagccgttt aatcgtataa
    18181 tctttccatt ttgaaatatc ttctgaactt ctccgcttct ctgtaaaaaa agaacggtaa
    18241 tcaactggtg ttaattgcaa atttccttta cgtagatttt cagcatatac ctcagtcata
    18301 aattcattaa aaagatcatc aatcataagg atggaaatca aattcactaa cttttgatta
    18361 tctatattta actgattgaa aagttgcaat ccagcttcat ccagttgact aactctcctt
    18421 ttcacgttct gatagatttc tttttgacgg ttactgctca ctgcaaaaaa aatattttca
    18481 tgttcatttt tctctttaat ttctatatct gtatatcctt ctagtcgtaa ttgaatatat
    18541 aacttgaact ctgaaaacca aaagcgttta gaaactagac cagcagaata ttttttattc
    18601 aatagattgc ctcctaatca tctataattc aataagtaaa caagtgtttt ttgctcatcg
    18661 ttagcattca ttttttagta tctactatac aatacatatt tattatag
//
LOCUS       DACDYJ010000052        18573 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDYJ010000052 DACDYJ010000000
VERSION     DACDYJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.052"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            464..1597
                     /locus_tag="IWM51_001897"
     CDS             464..1597
                     /locus_tag="IWM51_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP9971312.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            1628..1948
                     /locus_tag="IWM51_001898"
     CDS             1628..1948
                     /locus_tag="IWM51_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971313.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(1977..2441)
                     /locus_tag="IWM51_001899"
     CDS             complement(1977..2441)
                     /locus_tag="IWM51_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9971314.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(2573..3826)
                     /locus_tag="IWM51_001900"
     CDS             complement(2573..3826)
                     /locus_tag="IWM51_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9971315.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(3855..4172)
                     /locus_tag="IWM51_001901"
     CDS             complement(3855..4172)
                     /locus_tag="IWM51_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9971316.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(4198..4518)
                     /locus_tag="IWM51_001902"
     CDS             complement(4198..4518)
                     /locus_tag="IWM51_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9971317.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(5001..6416)
                     /locus_tag="IWM51_001903"
     CDS             complement(5001..6416)
                     /locus_tag="IWM51_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9971318.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(6433..7749)
                     /locus_tag="IWM51_001904"
     CDS             complement(6433..7749)
                     /locus_tag="IWM51_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9971319.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(7899..8597)
                     /locus_tag="IWM51_001905"
     CDS             complement(7899..8597)
                     /locus_tag="IWM51_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP9971320.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(8886..9875)
                     /locus_tag="IWM51_001906"
     CDS             complement(8886..9875)
                     /locus_tag="IWM51_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP9971321.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(9955..10788)
                     /gene="msrA"
                     /locus_tag="IWM51_001907"
     CDS             complement(9955..10788)
                     /gene="msrA"
                     /locus_tag="IWM51_001907"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9971322.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(10963..12231)
                     /locus_tag="IWM51_001908"
                     /pseudo
     CDS             complement(10963..12231)
                     /locus_tag="IWM51_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12506..13003
                     /gene="dfrG"
                     /locus_tag="IWM51_001909"
     CDS             12506..13003
                     /gene="dfrG"
                     /locus_tag="IWM51_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9971323.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(13075..15027)
                     /locus_tag="IWM51_001910"
     CDS             complement(13075..15027)
                     /locus_tag="IWM51_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9971324.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(15034..15330)
                     /locus_tag="IWM51_001911"
     CDS             complement(15034..15330)
                     /locus_tag="IWM51_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971325.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(15869..17716)
                     /locus_tag="IWM51_001912"
     CDS             complement(15869..17716)
                     /locus_tag="IWM51_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971326.1"
                     /translation="MFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKDNSSILKI
                     ELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRIKGLSS
                     IFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNICTILI
                     SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAYGLI
                     FNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLV
                     KAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTM
                     GLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWR
                     ILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHG
                     HIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNI
                     TYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKY
                     TPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            17703..>18573
                     /locus_tag="IWM51_001913"
     CDS             17703..>18573
                     /locus_tag="IWM51_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971327.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFI"
BASE COUNT         5806 a         3541 c         3010 g         6216 t
ORIGIN      
        1 ttatgttttt gacttggtta gacttattac cgcagatgca agcttttgaa caccgtttac
       61 caagaggtta tgaaaaaggc gtttagtttc gagtatgaag aaaaattttg tgaaaatggt
      121 tcctcacgtt tttgcataaa tttgacttaa taccgagaaa ctgatccctt ctgtcataac
      181 ctctttttat ttatgtctgt ttctttttga aaacaactat tttttcaacg tttactctct
      241 tgccaatcca acatcatcta acggatacaa ctatttcaat gattaccatt acactaaaaa
      301 atcggaaaca cacctctccg aacacaaata cataagacta tagaatgttt catttaagaa
      361 aaaaagcgtt tcattatcaa aaaggtaaga tctcctttaa aatgaaatta tctgtagtac
      421 taataatgca gacaatttta tgaatctgaa aggagcatct ccaatgaaag cagtcacttg
      481 gcaaggaaaa cagaaaatgg aaatcagaaa cgtagatgat ccaaaaatat tagaaccaac
      541 agatgcaatc atccgtatca cagcaacagc catttgcggc tccgatctgc atctttacca
      601 tcatggtgaa gcagttttgg aaaaagggta tgttgtagga cacgaaccaa tgggaatcgt
      661 tgaagaagtg ggatctcaag ttcaaacatt gaaaaaaggg caacgtgtgg ttatcccttt
      721 taatattagt tgtggtcatt gtcatttctg cctgaatcac atggaaagcc aatgtgatga
      781 atcaaatcca gatcagttat atggcgggct tttcggattt ggcaagttga acggtaatca
      841 ttggggtggt caagcggaat atctaagagt tccttatgca gactctactt catttgttgt
      901 accagataat gatcttcctg atgaaaaagt tttatttcta tcagatattt tgcccacagc
      961 ttattggagc gttgaaaacg caggagtaaa aaaaggcgat acagttgtga ttctaggttc
     1021 tggtccagtc ggtctattcg ctcaaaaatt cgctgttatg gctggagcag agcgcgtaat
     1081 cgcagtagat ccagtacagc accgtctaga taaagcggct aattacaatg gggttgaaac
     1141 ttatttctta gaagacacgg cacaagctgg aacagaattg tatgaactgg ctaaaggtgg
     1201 cgcagatgtc attatcgatt gtgtaggaat ggacggatta gagcctgtca aagaaaaagc
     1261 gaaaaattta gtaagcttac aaagcggcac aatctctcca ttacagatgg catcgcaagc
     1321 tgtcaaaaaa tttggaacag tccaagtaac tggtgtttat atgactccag cttcttctta
     1381 cccgctgcaa gaatttttta tgcgggatat cgctgtcaag catggacagg caccaattat
     1441 ccatctaatg ccaaaaattt acgcaatgat tgcagaagga ctattcgatc ctacacaaat
     1501 cattactcat tcgctgccac tatcagaagc agcacaagca tatgagatct ttgacaaaaa
     1561 agaagataaa aatataaaag ttgttttgaa accttgaatt cagtaaaaaa tacaggtgat
     1621 tttctttatg cccaaaatca aacgaaatac ccatgagatc gaaataggaa aagaagaaga
     1681 tatcgaaata cgaggacgtc gcttccgctt ctatttccaa aatcgttaca cattgatctc
     1741 actggctgtc gatttattaa ccgggatttt ctatattttt ggaagccttg cttccttaac
     1801 taaaatcccc gatcgatatg gcacctattt ttatttggct ggtgctattt tcttgacgat
     1861 tcgtcctatt ctcagaatcg taaggaacat ttttatttac gacgaaaaaa aagcaatgaa
     1921 gagaacaagc aaaaaaaaag aggcatgaga atttttcaaa tctcatgcct cttttttcac
     1981 cctatcgtat cactttgata cagacgagcg gtcgtcccac tttcttcctt gaccattgtc
     2041 aaaaatgtcc actcgtatcg ttcataaaac gtggtatgat ctgtaattaa gtaaagtttt
     2101 tcgtacccaa gttgatgcat ttcttttctg atttccgcta atatccgctt ggctatcccc
     2161 tgcttacgct tttccggttc aacaaacagg gcacaaagat ttggtgtcag atcagggcgg
     2221 ttatggaagt cgttttcaat gacgcccgct cctccgacga tggtttcttt ttcatctaag
     2281 accacatacc attgaggaat actttcctta ttagcgattc cagtacgaat actttcctca
     2341 tacacaacag caggaactcc ccattttgat gaaaaccaat tcgcgctttg ttttactaat
     2401 tctgggtgtt ctcttaattt gatccagtta tatgtaacca atgtttaaac ctccgactaa
     2461 aataaaacgt ttacaaaaat tatacaagtc tttatgaaaa ttagcaatga caacaatagt
     2521 gatgtaaaaa aaaaaacgga aaacaatcac ttgttctccg ttttttgatt aatcaagcat
     2581 ctaaagcttc ttttcttctt tcttggatcg tagctacctt gacgaacggt agatagatca
     2641 aaatagaaag aaccaaattg acagcggcta gtacaccacc tgcaatactt tttgttgcca
     2701 atacaccgcc aataattggt ggagttaccc atggaggcat aacggtagct gctggcacta
     2761 aacctgtaga agtcgcaaag taagcaacac taactaagac cattggtgtc aaaataaatg
     2821 gaatgaacat gatcgggttc aacacgatag gtaacccaaa catcgttggt tcattaatat
     2881 tgaagacacc tggcgcaatc gacaagtttg ttacaaccat atatggtttg tttttacgtc
     2941 ctactaaata aatagctaac agtaaaccta aagttgcacc tgttcctcct aagttgacaa
     3001 agctgtcaaa gaaaggtttg ttcacgataa acggtgcgct atgtccagct gcgattgcat
     3061 taacgtttgc ttcaatggct ggcgcattga ttgtttgcat caaaggatcg accatattcg
     3121 ctccgtgtaa accaaagaac cataagaatg gtgtgataaa tgcaagcagt aaggcagatg
     3181 gataagagtt tgcaagtccc ataaatggtt cttggaccac tgtatagaaa gaacctacga
     3241 tatcatcaac accgaatgca gcaaaaattg cagcaactaa agcaaacgca gagatagtga
     3301 tcattgctgg tagtaatgaa gcaaatgctt ttgcaactgc cggtggcaca ccatcaggca
     3361 ttttgatgat caatttttca tttccgctta aacgtacaaa gatttctgtt gaaataatcc
     3421 caacaataat agcaataaac aaaccgtttg agctcatacc taatgctccg cctaaagcga
     3481 agaaagagcc taaagaaatc acacctgcgg aaacaccgtc ttttcggtag ccgttagcta
     3541 aatgataagc aagcaagaaa gcaatcaaga cagaaagaat ggcaaatgta ccgttccaag
     3601 cagcagcccc gaatcctttc catgattcac caccaaaaat tgagttcatc aattcttgga
     3661 acccgggaat cggtaagtta ttgatcatta ctgctaatgc tcctaagatc attaacggca
     3721 tactcaccat aaatgagtcc cgaatcgcaa ctaaatgccg ttgtgcacca atttttgatg
     3781 ctacaggtat aaaatgtttc tccataaatg caacaaattt atccataaat cttccctctt
     3841 ttctttactg tagattacgc gttcaatcga cgatacagat caatgatctc ttttgctaaa
     3901 tctgtaaaag ctatagatgt catcagatga tcttgactgt ggaccgtcaa aagcgtgact
     3961 tcaacgtgat ttccttgtgc ttcttgtgtc agcatctctg tttgagagtg atgtgcttgg
     4021 actaaagatt cctctgcatc tttgattttt tcatctgcta aagtgaaatc accttttttc
     4081 gcagcagcaa tcgcttccat tgcatcgctt tttgcatttc cgccaaacat gatcagaccc
     4141 atgatggctt ctagattttt ttgatcttcc atttattgta tcctccgata atttttatta
     4201 tttgtttatt agtttttctg cttggtcgag tactttttca ccattcatca tgccatagtc
     4261 agccatattg attacatcta aaggaatgcc tttaggtgct agcttttcag caaattgatt
     4321 ttgcataaag cgtacttgcg gtcctaaaag taaaacgtct acatttttag attctagttg
     4381 attatccgca tctgaagcag atacagcaaa gatgtctgct tccattcctt ttgcttctgc
     4441 tgccttttgc atttttgtga ctaggagact tgtactcatt ccagctgaac atacaagcat
     4501 gattgttttc ttttccattt taaacacgtt cctttctata cttacacttg tatataagca
     4561 agtagcgtgc caactttaaa agcgctttaa aaagttgata tcatcacatt taaggtctat
     4621 accaagttgc atacacacta aaaaagaaag cgcacactaa aaaagaaagc gcacactaaa
     4681 cacacaaatt aatttttatt accttttttc ttttaacgtg cacaaaaaaa gttgtgacac
     4741 aaaacttgtc acaacttttt tcaaataaac ggtattcaaa agcttgcatc tgcggtaata
     4801 agtccaacca agttaaaaac ataagatgtt gtttttactt tgctattctt attacttgat
     4861 gcaggacagc tttttcacaa cctctcaata aacggtgttc aatcagctga ccctgggtat
     4921 tagctcaaaa cgctggattc acaacctctt ttatttcaaa taaccagtaa atacaatgaa
     4981 catctacaaa aaacttctat ttatttatag atttcgtcag gcaaatccaa ttctctctta
     5041 tcaatcaaag tctgatacca cgaagcagat tttttcaatg ttctgcttcg ttcatcgttc
     5101 aattcgattc gaactaatcc atatcgattc ttaaatgcat tcattggtga cacacagtca
     5161 gtgaatgccc ataacatata gccttcgcaa tttgcgcctt cttctgttgc tttgatcaag
     5221 taatgcaaat gctgtccgat aaattcgata cggtaatcat cttgcacgat tcctttctca
     5281 tccatatact gttcttcata ttctcttccc ataccgtttt ctgttatcag ccaagggata
     5341 ttatgataat tttcttttag atacatggct atatcataca tgattttcgg atagatttcc
     5401 catcccctgg aattattcat tttcttccca ggtaaatcaa agacttcata atatcgttca
     5461 ggatggaaag gtgtctcttc attccattga taacgagggg cgcgaacgcg tttaggataa
     5521 tattgattga ttccaaggaa atctactgta tgatgctgaa tcgtttctaa atcctctgtt
     5581 gcaggatcga aataaatcga ttgtttttga cacaactcca ggaactcttc tgtgtagtat
     5641 ccttttacca tcggatcaaa aaatactcga ttataaaaca aatcatacat ataagcagct
     5701 ttttggtctt ctggagaatt ggatcgagca tagaccattt caggatttaa aatacagcca
     5761 atccttccac cataatttcc gctgcggtaa atctctactg ctttagccgt tgctaaaact
     5821 ttatgataat tccacatcat ccactttttt gtgttttgtt catgcggcca gcgaacagca
     5881 tccaaatagc aacgcgtttg gggaacgatt ggttcgttga atgtaaacca gttttttacc
     5941 ctatcaccgt aacgatcaaa ggcaatttga gcatacttag cataaaaatc aactactttt
     6001 cttgaagacc agccatcata tttttccaaa agaaaagctg ggacttcata atgttccaaa
     6061 caaatcatcg gttctacgtc tgcttcgatc aaagagtcga tcatttcact tatatgctga
     6121 gcatattctt catctactat acagttctca taatcagtaa aaaaacgagc ccaattgata
     6181 gaagtccgat aatgtgtcag atggatggat ttcatcagat ccacatcctg ctggtattgt
     6241 tccataaaat tagttgctac ggctgggcca tacccattat gccaaacaaa atgttcattt
     6301 ttataccagc tatctataaa cgaatcctgt ccttcttttt ttcctttcca accttccgtt
     6361 tgccaagcag aagatgcagc accaagaata aaattttttg gaatagtaat tttcatgatt
     6421 tcctcctgtt ttttagatcc catcattttc cacacgattt gctactttta caaatggcag
     6481 atagatcaaa atagaaacaa taatacaaac aagttgcgta ataactgcac cgatatttcc
     6541 tgctgttgcc agataagcat tgacgatcgg cggcattggc caagggacca ttacaactgc
     6601 tttcgccgca aatcctaaag cggttgcaaa ataaccaatc gttccagtga taagaggcgt
     6661 taagataaac ggaatagcta aaattggatt cagcatgatc ggcataccaa aaataactgg
     6721 ttcattgatg ttgaagatcc caggagcaag ggataacttg gtgatttgtt tcatatcttc
     6781 ccgttttcca actaacaata ctgcaatcag taaaccgatc gtgactccag acccgcccat
     6841 actcatatac atatcccaaa atggcatagt aataatgttc ggtacttctt ttccagcttc
     6901 aaaagcttcc gtatttacag caattgccgc taaaagcaac ggttcccgta tcggcttcac
     6961 catttgattt ccatggatac caatcaccca aaagacttgc gcaataaaca tcaatagcaa
     7021 aatacccggt aacccttgta cgacaccttc taatgggcgt tgtacgacat tgtaaatgat
     7081 atcataaagg tacattcctg taagttcttt gatgataaat cccgcagccg cgatacccga
     7141 tacagtcaga atcgtcggga ataaagcaga aaaactagca gaaacattcg ttggcacggt
     7201 atcaggcatt ctcagctgta gtttcttctg tttcctcaac cagcaataaa gttcaataga
     7261 aaggatggag accaacattc ccaaaaataa ccctttcgta tctgtatatt gtttagccaa
     7321 tacgttttct actataacag tctgattttc attcactaat tcataaacag ttggattaac
     7381 agataaataa gacatcactg cgatgatacc agggaataca ccatccactt tattcagctt
     7441 ggctaattcg atccctatca gaaaaacagc atagatggtc aagaaactta gtgtgacata
     7501 gctgatggca gatgcgatag gctttaattc ttctaaaaaa cgcaagaaac ttatctgtgc
     7561 taagccattt tccgagtcaa agaccattgc tgaaaacaat gtgccaaaag cacccgtaat
     7621 aattaccgga atcaatgcag caaatgcgtt ttttatcacc atgatatacc ttaaattatt
     7681 gattctcgtt gccacgttcc ctaaaacatt ggctaattta tccataaaac tcattccaga
     7741 tcccctcatt cctgcttctt ctccttgacg tcatctcttt ttgtctatta gtcagcgctt
     7801 acatttcgat gaacagaaac tatttactcc tcttttttac caaatataaa acaaaaattc
     7861 atctgatgta tttatcataa aatacatcag atgaactgtc aattagtttc tttttctcct
     7921 attattttct gtaattctct ccgatttatt gccatatgga cagtcattgc atcaaacgca
     7981 gcaagtgcat tccgcctttc gatggcctga acgatctctg tatgaatatc gatcgtctcg
     8041 attttcagat gctggatatc ataattttta ttaaacaaag caattgattc attaatgatt
     8101 gggataatat gcagcaaggc tacgtttccg ctagcttctg caatcatcgt atgaaaatcg
     8161 atgtctaatt gaacatgttc ttgtgtgcct tctcgaaaag aagtttcaat cttttgcgtt
     8221 aattttttca tttggacgat ttgattcttc gttgcatatt ccgctgccaa acctgccatc
     8281 ttaggctcca ataaatatct cagttcaaat aagtctcgta tcatccgatc ctgatcttca
     8341 atcaaagaaa aaccgagtgg gtcattggca atccctcgct tatcactgac aaatgtcccc
     8401 gctccttgcc gaacttcaag gatattcctg gaagctagag cccgcacagc ttctcgcacc
     8461 gtgcttcgcc ccacctctaa ataatcggac aattcatatt cattcggcag tttatctcct
     8521 acttgcaact gcatttcctt taaatatcta aggatcttgt cagtagttac ttctactagg
     8581 gttttatttt cttccaaacc acatacctcc ttttcatctg atgtattttt tcgtaattat
     8641 tataacacgt ttgaatcatc tgatgatttt ttgtatacaa aattgattaa tccgttcata
     8701 gacatacaaa cgaagaagat atttaccata aaaaagacta ggataaaatt cactcctagc
     8761 cttcgatcat ttttattttg aacgacaaaa aatatgatgt tcaataacag acgatcttca
     8821 ttcgattctt gatgtcatct tttgtttttt ttaatatatg gataaaaagt ttgattacat
     8881 cgtttctatt ctagaatttg gtcgattctc aattcttctt ctggtgagaa atctaaatta
     8941 tccatcattc gaacactttc ttgtaactga cttaaccgac ttgctccgac cagtacactt
     9001 gtaacagatt tttgtcgcaa attccatgca agggccattt gcgctaatga ttgtcctcgt
     9061 gatacggcga ttgtttgcag tgctttgatt ttttccagag ttgatccaac ttgttcttct
     9121 gaaaggaatg gtatttcctt acgatgtgct ctagaatctt caggaatacc atgtagataa
     9181 cgatttgtca gcaacccttg agcaagcgga ctgaacgtga ttgcccctag cccttcttct
     9241 tctaatactg gagatagatc gtcttctagt agtgcacgag aaaacatatt gtagcgcatc
     9301 tgatgaataa tgaacggtgc ttcctttctt tttaaaattt cagtcatttt ttttgtatct
     9361 tctccattat agttggatat accaatatat aatgcttttc cttgtcttac aagttgcatc
     9421 aaagcttctg ctgtttcttc caaaggtgta tcagggtctg ggcgatgatg atagaagatg
     9481 tcaacataat ctagccccat tctttggaga ctttggtcac aacttgaaat gatggattta
     9541 cgagagcccc attccccata tggcccttcc cacatatgat aacctgcttt gctagaaata
     9601 atcagttcat cacggtaggc ttgaaaatct tctctaaaaa tacgcccaaa gtttttctct
     9661 gcagctccag cagggcctcc ataattattc gcaaggtcga aatgggtaat tcccatgtcg
     9721 aatgcgcctc ttaaaatctc tctttgatta tggataggat cataatcacc aaaattttgc
     9781 cataatccga gagataaaat cggtaatttc aaacctgaat gaccggattt acgatattcc
     9841 attttttcat aacgtgcact atctgcaata tacaattttt ctcctcctca tcaaatcttt
     9901 ttaaaacgct ttcttttttc tatttttagt ttactacatt ttaaagaaaa ggagttaatc
     9961 aaaatgattt tttcgtatat ttttttgcca tttgtttttt actcgttgat agatcagaaa
    10021 ttgattacgt gcttgctgga tttttttata acgtttcggt tgttttttgt aaaattgctg
    10081 atgatagttt tctgctggcc aaaacgtacc ggctggttcg atttttgtga cgattggttg
    10141 gtggaaagtt cctgaactgt ctagtttctg ttttgattgt tccgctaatt ctttttgtcg
    10201 ttcgacttga taaaaaatca ctggacgata ttgcttccct ctatcttgaa attgtccaaa
    10261 tgtatctgtg ggatcagtaa tttgccaata gattgtcagc aactcgctat aacttatttc
    10321 tcttgtgtca taaatgatct ccacagcttc cacatgacct gtgtaaccac cacttacttg
    10381 atcataattt ggtttttcaa catgtccgcc cgtgtatcct gacaaaacag ataggatccc
    10441 ttttttcgat tcaaacggct cgaccataca ccagaaacat ccccctgcaa atactgcacg
    10501 ttcttgatag tcagcatctt gcatggcatg cttggaaaag tcatattttt gctccttatc
    10561 aaatccttcc gtaatcaata aataaaagtc cattacatca ggcgtcagat tcattcggat
    10621 agctaatgga cgtagtgttg cttctagttt tgataactgt tctttaacgg atatattttc
    10681 tcttatgtca ttctttgtct gaatcaatac ttttcgttcc caatctcgtg tattcggatt
    10741 caaaatcaga ttataaagtg tttgaatgaa tatttgttcc tcatccatag attctcctcc
    10801 tttccgaaga agacaagcta gtgactttag ctgtaattta tcataaagtg aggaaataag
    10861 cgattacttc atccccctac agatttttcg tataaaaaag agagaatcct tatcgacaga
    10921 ttctcctttt taatcaattt attttataaa tgaaaagtta atttaccttt tatggaaaac
    10981 tattttaaaa aataaacata aatatgcagt taatgtaaca agataagaaa taatagtcgt
    11041 tactgtataa cttgtgctta ctttaatatc agcaactcct ttgactccgc ttatttgtac
    11101 caatccatta gcattcacct gctcctttac aggttgatga tttatttcta cattcgttcc
    11161 taaataagca tatacaggaa gtgtgattac ttggctcttc tcattagtta ctctatatat
    11221 gatagaacta ccagttgttt tataaggaag gtttacccat tgactgccaa tctttgcttc
    11281 gtgttttatt atatgctcct tattctccaa tgaagcgata ggagaataat catataaaaa
    11341 ataatccatt cctttagtgg tatacgcctc tactgtttct ttgtttacaa caatatgtcc
    11401 cggctgagaa attatttttt gattgaaatt ttttttacta gaaacagtta aagtgattaa
    11461 taacaaaaca gtcataaata aataaaatag ttctcttgtc ttattgtatc ttctattttt
    11521 gaaaaatatc atcgttaata tcagagaaga gaatattatt tgcaagctta agatccgcca
    11581 aggaaattgt aacaatttgg ctggcgtatt ttgcagaaga taccatggca ccaaactagt
    11641 tgtagctagc caagtcaaac acgtaccata ataaatcttt ttttctatgt ttgttaattt
    11701 tttaaataaa atcaggggaa tacataaaga taacagacca actagtccca ttgagtaagt
    11761 agttaagtcg ttgttcatag cttcagtcag actatcgaac acattagatg catgagcata
    11821 taaatttgtc acgctaggtt tgttgatttt ttggtagagc atttgttcaa acatcggata
    11881 ccaaaaagca ctcgtcagaa ccaatgtcat tcctgccgca ctaaataatt ttttaaatat
    11941 agcaataaaa tctgcattct tgaaaccttt agaaaccaaa cgaataaaaa taaaaatgat
    12001 aattaatgct gtactcatta atgttgatag tacatggctg tacactagca aactcatact
    12061 caatgctagc tttacagacg gatacttttc ttcttttcca gagatgattt gataagcata
    12121 aaataataca gggataacaa gtgttattgc gatagcttcg gcaattgcgg aacgatagta
    12181 gatgtctact gtccgataag cagagaaaat atagagacaa ctaaataaaa ataagtagtt
    12241 cagttttgga gtacaaaatg acaaaatgta cttcaaaatg gactactttt ttcattttaa
    12301 cttacatacc aaattacata caaaatgatt tataaaatgc aattgaaaat gataattgaa
    12361 tgaaaatttg tgattttgtg gtattatgat aatataactt atagaaaaaa ttgcttattc
    12421 aactaacggg caggataatg agaaaaggtt ttttgataga aataagaaat tagatggtaa
    12481 attagttaaa aaggctgtgt tataaatgaa agtttctttg attgctgcga tggataagaa
    12541 tagagtgatt ggcaaagaga atgacattcc ttggaggatt cccaaggact gggaatatgt
    12601 taaaaatact acaaagggac atccgataat attaggtagg aagaaccttg aatcaatcgg
    12661 aagagcctta cctgacagaa gaaatattat tctgacgaga gataaggggt ttacctttaa
    12721 tggttgtgaa attgttcatt caatagaaga tgtttttgag ttatgtaaaa acgaagaaga
    12781 aatttttatt ttcggaggag aacagattta taatttgttt ttcccttatg ttgagaaaat
    12841 gtacatcaca aaaatacatc atgaattcga aggagatact ttttttccag aagtgaatta
    12901 tgaggaatgg aatgaggtat ttgcccaaaa agggataaag aatgataaaa atccgtataa
    12961 ctactatttt catgtatatg aaagaaaaaa cttattgagt taacgggtgc gttaattgaa
    13021 gaagaacagt aaagataaag cacaagagct tgggattact ccaagctctt tttgtcattt
    13081 gaaataaatt atatctttcc aatcaaattg tttatctgta atccctaatc tttgagcagg
    13141 tgtcaaaacc tttttatccg ctgatttaaa agggcgacaa aagttgtagt aagttcttag
    13201 aattgtaatc gcaaattgag catacttcgg attgaagtta gcgtaaatat agctcttctt
    13261 atcaccacga gctgttgtta atggtctttc aagagatgat atataacgtc ttatctgttg
    13321 aataaagcta ttggtcgaat ggtcattaac atttaatagc atctttgcga tttcgtttgg
    13381 ttccagagca gataaatccg ttctacaatc aactgaataa aatcctttat ccttagtggc
    13441 taatggatgt ttgatagggt tatcggcata tttgcgatat ctttctccat ctttactaat
    13501 agcctcttcg tggaattgat gacttgttag taattcagtt agataatgag atgccaaagt
    13561 ccgtagaaac tttgttttga atcctctgat atctccccaa tcgagcagtt ctgcttttgc
    13621 ttgtccgaat tcttttaagc attgttttct gcttttcgtc ttgttaactt ggcaaataaa
    13681 atgatgtgca tcgctcaacc ttaattcttt cgtgaatact ctataaaagg cgtttctaat
    13741 agagaagtca tcatcactaa tcattcgcca ttcgtcggca ttaaccattt gtttaattaa
    13801 ccaatagtga gcagttgtag tataaggagc atcaacattt aaaccatcga caaatctact
    13861 cctattagta attttcccta attcgtgaag ataagcattc ctattttcgc tatcgttagc
    13921 ggatggttct tgtggataat aagaccaatc taacctatca tttttacgac taaaagtatt
    13981 gagatggtct tctttgaatt ttcttgtatc ttcatttaat tcatccattg aaatattcca
    14041 atcataggca acatcagaac ggaacacata tcttgataag acctcagcag atacaactat
    14101 atacgtttgc atattaaggt cttcaaaccc cgtatatttc tttgaacctt gtcctttctt
    14161 acggacgtta tttaggtagt agtgcatctt gtccgtatta agccacatct cgttaaattt
    14221 ctttgtttga aggggttgtg tctcatatcg ttctaaaaac tccaaacaac gcctatacag
    14281 ccactccagc ttatgatagt aggttccaac tccaattcct aaaatatcac aagtacgact
    14341 gactgaaacc cttccaacaa ccatcttagc gaacattgga agaatggtgt tcttttgttg
    14401 gtgataagta gtggtttctt ctctttttgg tagaacatta gtgaacttct tacacgcctt
    14461 acattgatag cgttgagatt tgcccctact tttccccctt ttgtaaaatt gcttcggctc
    14521 attgaatggg gttgattctt cttccgagca gccttcttta tgaaattgat aatctggctc
    14581 aacatcttgg atgctattga tttctataag gcgtttaatt tcttcaacaa cagaccaatt
    14641 agataaaggg gttactgtac agttttgcga tacacctctg ccaataggat tgtcattgca
    14701 gtacatacct ttatgacctt tatctttgga actaccagtc attttatatc gacttggttt
    14761 cccttttaca tctttgaatt tgtgctgttc tttcccaaaa ttacaacaaa acggattgat
    14821 gcagtaatta tattgaatgg gatattcaga accgttccat ttaaaagacg ttggcaagaa
    14881 caacattttg ttataccttg ttgcaaatcg ttttggagtt aataaatcat attgtttagc
    14941 cctatctttt aattcttcgt ttgaaatcgg aattttaaca tctacaattt catcttcctt
    15001 cgtggctaat cttttaagct tggtcattgt ctatcaaccc actctatctt tgatacccat
    15061 tttttcaaca atccgcttat tacttcgctt gttggcaatc cattcaataa acttcttatc
    15121 gtctaaaatg ttcaataaga attcatccac tgcttcgctt tcagtacgct ctgcatattc
    15181 agcgtattgc ttaactatca cccttacttg ctctgatatt tcccaatcca cagacttagc
    15241 gttcttattt tttggctcaa gaaattgcat aggaaaatgc accccttttt gcttagtaaa
    15301 ttgtaaccat tttaacacac caatttgcat tcctacaata attatttcac gttcgcattt
    15361 ttatatacat tttaacttac attttgtggt gtaaattaac aacaaacgtt gatttaacaa
    15421 taaaaaacga gtatcaaatt cagttgcaat ttgatactca tttaggttta cattttcact
    15481 ttttgtactc caaaactgaa ctacttaaaa taaaaatgcc gctttttttt gttttaaaaa
    15541 ccgctcgcca taatgataag ctacgagtgt tgtacacaag ttcaataacc aaacgtaaat
    15601 aatatagctt acaatcaaat tttttgtcat ccaatataac attacagctg gaaaaaaagt
    15661 cagaaaggga taaaaataat ttacaccata tccagtataa ttgaatgtct cataattaat
    15721 cggactggta aaaatagtcg ataacccctt gattcgagat aagtgaaaag aaagatctcc
    15781 agtggtataa atactctgtg tatttttgaa aaagatgaaa gaatatatac tactaactat
    15841 aattaatcca ctcaatatta ctactttttt atttattttc attcttcttg tttatctcct
    15901 ttttagtcac atatcgagaa tctttcaact tattaggtgc aaatcgttta aaacttgaat
    15961 acaccactaa acaataagtc agaatactga tccataaagc catgagagca gttggagtat
    16021 attttgttgt aatttctatc ttattttctc cagcatttac atccacattc actaagccat
    16081 gttttttata agagtcattt acgactttac cgtttacttt tacctgtgta cctaaataag
    16141 catatactgg tactgtcact ttttgctttt tgttgcttgc cactgtgtaa gtaatgttat
    16201 tatctgcagc tttatacttc cctttttccc acgattctcc tacttttact tcatgttttt
    16261 ctaagtgagg cctttctttt atcgcttctg ttggggcgta atcgtataat cctcccatat
    16321 ctgatgttgt ataacgtggg agtgtttctt cattgataac aatatgaccg tgttttgatt
    16381 ggataacttt tgcgtaattt tcttttgctg ctatagacgt gacgagtaga aacaccacag
    16441 tagcgccaat gattaaccat gaatattttt tattcatagt ctttttcata aaaacaagcg
    16501 ttatgatcat tgaagaaaaa aagatttgga tacctaagat acgccaagga aactggagta
    16561 agcttatcgg tgttttttga aacaaaaacc atggaaataa attagttacg catacccatg
    16621 tagaaactac gccaacatag atgatttttt cttttttctc catttctttc agtatgaata
    16681 aaggaaaaat caacgcaacc aatcctaata accccatagt gtatgttgta atatcgttat
    16741 ttaacgcttg aatgatacta tctgaaatag aaagtgcttc tagctgcaaa tctgttttga
    16801 agggactgtg tatttcttga taattcatct gctgcaacat aggataccaa aaatagctag
    16861 ttaatgcaca agtcaaaaaa acagccttta ctaaattcac gaccacaaat tttatatcgt
    16921 tttttatttg cttgcttaaa aacaaacgaa ctataatcat acaagcaatc aatgcactac
    16981 acaatagtgt agttaagaca tgagtataca caagtagact tatactgaca gataaagcga
    17041 tgactttttt ttctctattg aatatcaatc cataagcata atataaaaca gctgggataa
    17101 tcgtaagagc gattgcttct gcaattgcgg atcgataata gatatcaact gttcgataag
    17161 ttgcaaaagt atataaacaa ctaaataaaa aagcagcatc tatcttttta aaaaactttt
    17221 ctccataata aaaactaatc agaatcgtac aaatatttaa taaccaaata tatagaacat
    17281 aacttaatat taagttattt gaaattccat aaaaaacgac tgcaggaatt attgtaagaa
    17341 aaggataaaa atagttcagt ccatctccat aattgttaaa tgttgtatag ttgattggcc
    17401 cttcaaaaat agaagataac cctttgattc gtgaaagatg aaaagataaa tcacctgttg
    17461 tataaatttc ttgagcattt ctcataaaat ttactgaata taaagtacta agtaagataa
    17521 gaactactaa aagaaatgct tttttactta ttttcataga tttgttgttc tcctttttta
    17581 attctatttt cagtatacta gaattgtctt tttttaataa gttttgcgtt aaatttttca
    17641 tagcactagt atgatatact agaaaagaat caatttttat tacttttaaa taggagaaaa
    17701 aaatgaaaaa aaacaaaaac caaataatag atatcatttt tatgttattt ctaagttttg
    17761 cttacgtcat ccctctattc atgtctaagg gaatctatca tagtgtcaat caggatactt
    17821 actttcattt atctcgaatc ataggactag ataatgtatg gagttctcca gtaaacttca
    17881 ataacttcga tcatcatgga acaatgatga atatttttta tccatggctt actctttatc
    17941 cagctttttt attttataaa atgatgggaa atcttgtttt aagttataat atctactatt
    18001 tttttatcac ttttttaaca atggtcgttt cttatttttc tatgaaacaa ataaaaaaca
    18061 atcgttatat ttcgctgtta ttttcgatta tttatacatt ttccgcctat cgtgccattg
    18121 acatattcca tcgtgcttca ttgggagaag ctgtcgcttt aacatttttg cctttgatac
    18181 taatggggtg ttacgaaatt tatattagag attaccaaaa atggtactgg ttgtctattg
    18241 gcatgacgct agtagtgtat actcatttac tttctgtcgc tatggtttct gtatttattg
    18301 gaggaacgtt gtttctttct ttttattttt gggaccaaaa aatcgctaga ctcttaagtc
    18361 ttttaaaagc taccgtcctc accttttttt taagtgcggg attcttaatt ccttttattc
    18421 aacaatctag agcacaagaa ttgaaagtcc cactgggaaa agaattaagt ggtatggctc
    18481 ctagtgatat gctgacacat attttaaata ataactataa caactatact attggattat
    18541 tcttatttct cggattgatt ggtgctttta ttt
//
LOCUS       DACDYJ010000053        18345 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDYJ010000053 DACDYJ010000000
VERSION     DACDYJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.053"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..134)
                     /locus_tag="IWM51_001914"
                     /pseudo
     CDS             complement(1..134)
                     /locus_tag="IWM51_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(176..1302)
                     /locus_tag="IWM51_001915"
     CDS             complement(join(176..1074,1074..1302))
                     /locus_tag="IWM51_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9971328.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(1381..1899)
                     /locus_tag="IWM51_001916"
                     /pseudo
     CDS             complement(1381..1899)
                     /locus_tag="IWM51_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1964..2800)
                     /locus_tag="IWM51_001917"
     CDS             complement(1964..2800)
                     /locus_tag="IWM51_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9971329.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2818..4227)
                     /locus_tag="IWM51_001918"
     CDS             complement(2818..4227)
                     /locus_tag="IWM51_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9971330.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(4241..6064)
                     /locus_tag="IWM51_001919"
     CDS             complement(4241..6064)
                     /locus_tag="IWM51_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9971331.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6907..7233)
                     /locus_tag="IWM51_001920"
     CDS             complement(6907..7233)
                     /locus_tag="IWM51_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971332.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(7279..7641)
                     /locus_tag="IWM51_001921"
     CDS             complement(7279..7641)
                     /locus_tag="IWM51_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP9971333.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGSLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7678..7893)
                     /locus_tag="IWM51_001922"
     CDS             complement(7678..7893)
                     /locus_tag="IWM51_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9971334.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            8020..8355
                     /locus_tag="IWM51_001923"
     CDS             8020..8355
                     /locus_tag="IWM51_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9971335.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(8431..10428)
                     /locus_tag="IWM51_001924"
     CDS             complement(8431..10428)
                     /locus_tag="IWM51_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971336.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENIEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(10526..10801)
                     /locus_tag="IWM51_001925"
     CDS             complement(10526..10801)
                     /locus_tag="IWM51_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9971337.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            11034..11768
                     /locus_tag="IWM51_001926"
     CDS             11034..11768
                     /locus_tag="IWM51_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9971338.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11807..12949
                     /locus_tag="IWM51_001927"
     CDS             11807..12949
                     /locus_tag="IWM51_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9971339.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     ARESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(13044..14435)
                     /gene="sufB"
                     /locus_tag="IWM51_001928"
     CDS             complement(13044..14435)
                     /gene="sufB"
                     /locus_tag="IWM51_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP9971340.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14440..14910)
                     /locus_tag="IWM51_001929"
     CDS             complement(14440..14910)
                     /locus_tag="IWM51_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP9971341.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14897..16132)
                     /locus_tag="IWM51_001930"
     CDS             complement(14897..16132)
                     /locus_tag="IWM51_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9971342.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAH
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     SFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVINPVQELAELVHENGGVIVVDGAQSAPHRAIDVQALDCDF
                     FAFSGHKMCGPTGIGALYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQELGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(16129..17415)
                     /gene="sufD"
                     /locus_tag="IWM51_001931"
     CDS             complement(16129..17415)
                     /gene="sufD"
                     /locus_tag="IWM51_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP9971343.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSTVDQLGKNITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRQELVEVIEGKLNG"
     gene            complement(17428..18201)
                     /gene="sufC"
                     /locus_tag="IWM51_001932"
     CDS             complement(17428..18201)
                     /gene="sufC"
                     /locus_tag="IWM51_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP9971344.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFNGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
BASE COUNT         5457 a         3775 c         3073 g         6040 t
ORIGIN      
        1 gtgcgatact cagacgcatc attcagtaac aaccgaacgg ttgtcgtccc ccaacgaatg
       61 atcctagagg ggacgacaca aagacaatgg gaacactctt ctggcttgta cagaagagtt
      121 cctttgtaaa tcatggattt gagtcaatat cttggacaca ttttagtaga gacggttaag
      181 ccattttttc ttttgctaat tcttcaaatt cctgtggggt tagatagtga atcgagctat
      241 ggattcgatt tcggttataa aatccttcaa tgtagctaaa tagcgctcga tttgcttctt
      301 caaaatctga gtagctagtc gtgtagactt cttctttttt caatgaggca tggaaagatt
      361 cgattccagc attatcataa ggtgttcctt ttctactata agaatgcctt attttgttgg
      421 ttttaagcca ttgttccact tctcttgctg tgtattggct gcccaagtca gtgtgtagaa
      481 tcattccttc tggaatgtga taatgtattt ttgctttatt caatgtttga atgacacaat
      541 ccacagtcat tcgttttgaa aatgtataac taataatttt tttcgtgtgt aaatccatga
      601 ttgaagataa gtaacaccaa ccatttttct tagtaggaat gtacgtaatg tcagctgccc
      661 atttctcaca gatcgtttca gtagaaaagt cctgatttaa gagattctct tttgaaatga
      721 tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt aaatttaatt
      781 gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt aataaaactt
      841 gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct
      901 ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta taaaaataat
      961 agacacttct agggatttct aataaccgac ataacaaaga aacagtgtaa tccttacacc
     1021 atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg ctgcggcttt
     1081 ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt cagctacttc
     1141 ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt tgtaaatcgt
     1201 tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag tttgattcag
     1261 ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc atcctctcgt
     1321 agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga tactaacacc
     1381 agtttcgatg aatgaatcaa ttctttcatc tatcgtcttc ccagtatcta cttctgtctc
     1441 attactctta gatgaatcga ttctttcatc catcgtctgc tccgtaactg cttctgtctc
     1501 attattctca ccggaaactc tcttcacacc caaacttata tattgtccat ctttagtgtg
     1561 catagttaaa attcccacgc ttttactaat aaattcacta taaccagttc tttcaataga
     1621 gacttcaatg tatttcacac tatcatactc aaaagaaaaa tagacatgta aaagcatttt
     1681 gttgtttaca tctacataat aatcctttga ctctacttga gaccttccta tacggaatac
     1741 agctttactg tctgatatct ctataccagc atccttatta ttaatctgcc aatctccaag
     1801 tagctcttct ggcaagctag tagtttcttg tgaacacttt accacttcac tggtagaggt
     1861 cgaaacattc acctccgcac tgttagaaga attttccaag gaatcaggag ccttagcaca
     1921 accaacaagt ctttatatat tgtgcagccg atattttctt cattcagtac acccttgaaa
     1981 tatgcagtgt tagataaagc agttcgtcct gatttgctga ggcacccatc tcttcttgaa
     2041 cgtactcctt gattttcaag gccgcccgat actctttcgg aaaacgctgc ttcactgaag
     2101 ccagcaaaaa atcatccttc tccggcaact cttccttctt aatcattcgg gcaataaaaa
     2161 actgtaaatg cgtcacaaat cttaagtaaa gcatcgaatc tttatcaacc tttttttcgg
     2221 gaaacagata tcctagcata gccaagatat cctttagtgc tcttaccgag agcatggttt
     2281 cttccatatt tgcttgttca ctttgggcat ttacaatgtg aaacgcaatg tttccagcct
     2341 cctcttctgg caactgaaca ttcatttttt tgttgatcaa ttggatagca tagcagccga
     2401 tctcaaattc ttttgggtaa tacttttgaa tttcaatcag caggcgattt tgaaaaacaa
     2461 tgcctttttg actgcgttca atggcatgct tcaagtgatc aaccaaggta aaaaatagac
     2521 ttttattcag cttaaagtca aacttggcgt tggcataatc gataattcgc ttgacgcaaa
     2581 taattagatc atccggagca gcctcaaaca gtttaatgta atcctttttt agagcatcag
     2641 aaagtgaatc taatttaaat actttttgga ttttgtcagc cggaaccaaa tcgcctttct
     2701 ttagttgaaa tccaatccct ttccccatca aaattacttc tttgccttgc ttatctaagc
     2761 aaagaattaa actgttgttt aacgttttaa ttactctcat tagattcacc gttctctcta
     2821 agctaagcca tttcttgcga ttactttttg ataccaaaag aagctttctt tttttattcg
     2881 ttttaattct ttaagattct catcggttcg gtcaacatat ataaaaccat aacgcttatt
     2941 gaagccttga tgggtactaa caacatcaat cgctgaccaa ggtaaataac caaaaacatc
     3001 tacaccatct gttaaagctg cttgaatagc cagaatatgt ttggctagat aatcaattcg
     3061 atcctggtca ttaattcgtc tatcttcctc aactatgtcg ggtgccccat agccattttc
     3121 agtaattaaa ataggtaaat catagcgatc gtacaatttc ctcaatgtta atcgtagtcc
     3181 agtagggtca actacccagc catatggcgt tttatctaca taaggatttt cagctgcccg
     3241 gtagacacct tcttctccca acatgatctg ttgatccccc gctctagcgg aaacatctga
     3301 tccgtcattt ttgctggcag cgatggtggc agttgaataa taattaatcg caataaaatc
     3361 gggtttaccg gaggataaaa ttgcctgatc ctctgcctca attttaggat ataatccacg
     3421 atcattcaaa tagttttcaa acaaataatt gtattttccc cgaactgcta catctaagaa
     3481 agaccagcct ctcaatgttt cccaattatg ggctgcaatt gcatcttctg gactggaggt
     3541 agcttgatac atagccgtta aattgattgc tggaccgatt tttcctttaa gattcaactg
     3601 atgatacaag tgaattactc tagcttgagc taataaaaca tggtgattaa tttgaaagag
     3661 ttcttttttt gaaggcagct ctccacttgg tggcaaacca atcgctccag gatgaaggat
     3721 catcgtgttt tgctcattaa ttgtcagcca atagttcacc ttgtcaccat actcagtaag
     3781 caatacctgt acatagtcca caaaataatc gatagttgct cgatttaacc aaccgccctg
     3841 ctcctctaga aaataaggta aatcgaaatg gtacatggtg acaatcggtt cgattccctg
     3901 attcaggcat tcatcaatta cttgatgata aaaagaaatt cccttttgat tggttttatt
     3961 attttcaata tccataattc gcgtccaagc gatggaaaag cgaaaagctt ttaagcctaa
     4021 ttctttaaac agctgtatat cttctttata gtgatggtaa aaatccgagg caacttcaaa
     4081 attagctacc ccttctgtat gctgatactt gtcaataata gacggttgtt tcccatcctc
     4141 ctgataggca ccctctgcct ggtaggcact aatggaggca ccccataaaa acttctctgg
     4201 aaatgctgaa agctgtttgt attgcacaaa aatcctctcc ttaactaatt tgcatcaatg
     4261 gttcgtgttc ctttaagctt ccgcttgctt cactaattcc tgcgtaattt tcactgttgg
     4321 tcactaccac aatcactgta tcatcgtagc cagcgtttct aatggcagcg aaatcggcgt
     4381 tgattaataa ttgtcctgct gacacctgat ctcctgcttt tacaaatact tcaaagggtt
     4441 ccccttttaa attcacggtg tcgatcccca catggatcaa taattcaata ccttctttag
     4501 aacgtagacc aatcgcatgt ttagattcta aaatagtaat gacttctccc tcaacaggtg
     4561 aataaatgct gttttcttct ggatgaatgg ctacgccctt tcccaatagt cccttagaaa
     4621 agacagcatc gttcacttca gaaataggac acacggttcc ttttaccgga ttttcaacaa
     4681 tgactgcttc ggctattgcc gtttgctctg attcatcaga agtatcgtca aatccaatta
     4741 gataagcaat gataaaggtt gcaacaaagg cgattacaca cgtaataatt gcgtgaacaa
     4801 tattcattgg attttctcca ataaaggctg gtaaggctgc taagccaggg gaaacaaagg
     4861 catatctgac taaaccgctc aagccagcat acaaaccagc aattgcacca ccaatcatgg
     4921 aagcaactaa tggttttttc agcttcaagg ttactccata gagtgaaggc tcagtaattc
     4981 ccattaaagc agtgatccca gtggacacag ctagctgctt caactctttg tttttagttt
     5041 tcgcggctac agcaaaggta gcacctgatt gagcaatatt agaagccagc attcctggcc
     5101 cattgatcat ttcagaaccc gtactgctca attgcatagt agcgattggc gtcattgccc
     5161 aagctgtacc cgtaaccacc aaaaatggct gcaaaccacc catcaaaaat ggaattaacc
     5221 aagctgcttt gccgttaatc cattcagccc ccattgctat aagatcgttc agcaacgttc
     5281 caaacggtcc aatgacaacc aacgctaaag gccccataat taaaaatacc agcatcggct
     5341 tagtaaagaa tttaataaac gaaggcgaaa ttcgatccgc aaagggctct acataggaca
     5401 tgatccaaac agtaaagata atcggtaaaa ctgatcccgc ataatctgca gctaataccg
     5461 gaattcctag aaaagaaacg gcatcgccag tagaaaaaag ctgtgaaata tttggatgaa
     5521 gcaaaatgcc agcaacagta atcgccaaaa ttgggttgac atcaaacttt atggctgccc
     5581 cataggctaa tagtactggc ataaaataaa aggctgcatc agcaatagta ttcaaaatga
     5641 tgtaagtttg actactgtct gatagtacac cagtcaaagt caagacggcc aaaatggctt
     5701 ttaccatccc ggcacctgtt atagctggaa tcatcggtgt aaaggtggta gaaatgacac
     5761 taatcaatct tgaaataata ctttcttgat tgtctgaaga ggggcccttt ttaattgaag
     5821 ttcccacttc cttttgaatt tccttgaagg ctgtttggac ttcattgcca atcaccactt
     5881 ggtattgtcc accgctatcc accacactaa ttacaccaag tgttttttct aaggctttcg
     5941 tatcggcttt ccttttatct ttgagttcaa aacgcagtct ggtggcacaa tgggttaagc
     6001 tgaccacatt ttcttttccc ccaacctgtt gcaatacatt tcttcctact tctctgtaat
     6061 ccattattct ctctccttaa agcagtaaaa aagaccaagt caaaccacgc cccaatcaac
     6121 attgaagtgt tagtttgtct tggtcttgcc tgcataatca gtaacatgcc aataacgtta
     6181 ttttgctgta atccaataat agcacattat gatagcgcaa tcaacaggga atttaatttt
     6241 ttatcttttc gtttgaccgt atagggtaat ataacagtta gatcacatca actagggtat
     6301 atctgcgtat acttactgct aaaataagta tatctaatat taactatacc agtaaaacca
     6361 ttcctacata accagcggga aattcccaaa atcgtgaaag catcaaaaat gcatataacg
     6421 gatcacacaa cttcaacaat tctgctcttg ggaaaaattg atgaatttgg tgagtaaagg
     6481 atggaataaa cagcattcca acaatatagg caacaatagt gatacttgtt tttttggtaa
     6541 aaatcagtaa gcaatagcca atttcaatca ttccgagata taaaggtatc gatagaatga
     6601 tttgggctag tattcttaaa aattaactag caaatgttta aactgattta tttctaatct
     6661 acatgtagta agtatttcaa ttccaccaat tatcaataaa aataccaaac atatccataa
     6721 taattttttc tttttcattt cttttatatt gttataacag tgaataacat aaaaattccc
     6781 gacaatcatt gtaatatttt tttgtcacct gtattagtat ttaatttata ggaaactata
     6841 aaaactccct agtgaattta cacctttggg agttcctgtt tggacggtat gcataataca
     6901 tttaaatcaa tttaacatta acacctcagc atatggtatc tccacatata tacggtcttt
     6961 ctttggatag tactcaatat ttatgttgat agcacttcca ttgtctaata tagctttagc
     7021 ccggtatgtg ttatctccat tttcatgagt aatcgtcaca tcatcacatg atcgatttaa
     7081 gttgtactgt tcttgtaaaa tctgtgttac caatggtttt gatgacttct ctaatccaga
     7141 tgtacctgtc atttttgaaa aaataatacc tataatagcc aagacaacaa tacctataat
     7201 gacaaacttc ttgttcatat ttactttatt caacttttta tctcctatct ccaaaataat
     7261 ttgctcttat ccctcttatc agtacattga catccctaaa agtacaccaa agattgctcc
     7321 agctacccct gctatcatca aaatcgtacc cattttctca tctttttctc gatataagga
     7381 gcccataata acagcaacag caccaaatac aacaggaata aatgctaacg atactaccat
     7441 agaaatccaa ccaataatca aatacagctt ccctgtctct tgtccatgta cgatttgttc
     7501 cttcatttca ttaattgtct gctcagtact tttctttact gtgtctgttc cggttgtctc
     7561 atctgtttct tgtagtgctc cacaaaactg gcaatactta ctttcatctg ttatttcttt
     7621 gccacatttt ctacaataca tgtcttctcc tcctaaaatc attcaattgc ttattagtta
     7681 actgtttact ttattaaata catcagaaaa cagctcattc attggtacac ttaatatctc
     7741 agaaagcatc ttcatttgat agggtttagg aatcgttctc cctgtttgcc acgaacttac
     7801 acattgctgt gtaacattca ttttttttgc taactccgtt tgagaaagtc ctttttcttt
     7861 tctgtatcgg ctgagattac ttactttttt catgattctc ccctcctgtt ttattatata
     7921 caacaatatg atgtatttca actcataaaa tataaaaaac ttcaaatacc attacaaata
     7981 caacaacttg ttgtactata ctgagatggg ggtgtattaa tgaaacagaa acaatatgca
     8041 aaggtcatta gagaaaagcg aaaagctctc gggtggacac aaaaagaatt agctgaaaaa
     8101 atcttctcta cacaacaggc agtagcaaga tgggaaaatt ctgtaacaga gcctaacctc
     8161 gatagcttaa cagctctttc tagagcccta ggaacccctg taagccattt tctagacaat
     8221 gttgtcgtag attatgagga agagtttttg gctctctatc gttctcttag cactgaagat
     8281 gcagttcgaa caattgacta tatgaagcta ttaaaaaggc aagaaaatga acgcaatcag
     8341 ctattgaaag attgaaaaat aaataaccca ccgaacttta acggtgggtg tattgagatg
     8401 aaataagatt aagagctatg gataacctgt ctagtttaat acaattgggt cttttctctt
     8461 ccttactctc cgtccatcat ctcttgttac acttgtaaat tgatacagag gaactatccc
     8521 agctcctgat aatatcaatt cttttattgt ttcatctatc cctgcccctt tcgctaaata
     8581 tattaaaaaa ctactacttg ctttcacatt gtatagtttc ttaatatcct tagaagctcg
     8641 ttcaagagtg aatccttgtt taacaaagta gcctctgttt tttattaaag catttttcac
     8701 gctgttatcc ccaacattac gtaggcgttc acaaacagtg ttaataagtt ctttctctgt
     8761 cattagatat gtcccttgcc acgataagcc ctgtaaaact tcatgtgctt catctaaaat
     8821 caattgtttt tccttgctga gttctgatcg ggaattttga ggaaatactc ttttagcctc
     8881 tttttcgtta aaacatatct taacccattc ataagaaaaa tattgatatg taaaatgaat
     8941 tttttccaga agtggaagga tatcttcatt caatttttga atcacagaag ttgcattaaa
     9001 aacgtctaat cgatagacat tgcttctctt atgattaaag tttcctaacg ctttctcttc
     9061 ctttaaccta tttaggtatt cgagcatctt caaaggaaca tcttcatcat ctaatttttt
     9121 tatcaatcct ataaaagtca taagtgatag taacttatta catttatcta tgtcataagt
     9181 tttaccatag taagaactca tttttttagc taattgttcc cccgacaaaa tacaagtcac
     9241 actttcctta ctatcatcaa aaaaattaac atcagaaagc aagacatcat agacacgtcg
     9301 catttcctct agataattcc ttattgtcct gaaaagtttg tctttcattg attcaagagt
     9361 gtcaaaaagt acttttgatt cctctttcaa ttttcgaaaa tgtgtaggat acaagtctag
     9421 gttctctaat agccaattaa atgtttctac ccgactgtct ttctttaaaa ttagcatcac
     9481 aactccaata aaattttcac tgatatcaca attactggaa tgacaataaa aaatggaatg
     9541 cccagtttta ctggtaaaaa tactagcaga gggattatga tcgttgtgaa acagacatct
     9601 taagttttta cttgacttaa gtaatttatt catatcaatg gttaacaatc tttcataaat
     9661 ctcattaaaa gacatgtcgg aggaatcaaa caggcagtct ttaaaccact gtttcatttc
     9721 ttctttgtcg tcatttttta tcaacttaac aaaatcggtt tcattgacta gttcatactc
     9781 tctccctgaa tcactttgac aatttttaga acatgtcatg gtaaaatcaa tcggaagctc
     9841 agtaatatct aacatgttgt cttcattaat tattatcact gcatctgact tatatcctcc
     9901 aaaaaacatt ctattcgaag catttacatt gtcatcacac ccggggatgt tctttttcaa
     9961 gtattcgtaa acagcaacca ttttaccata gtcagtaatt gcttctttta atagaaagac
    10021 aatcttgtat ttttctatgt tttcagagta ccgaatactt ttttgtatcg caaacgcaga
    10081 attatgaact aaattgatag ataaaacctt ttctatgctt aagtatctac tatcatcttt
    10141 actcaaatct actaccttcc ccttctcatc tttaacagtg ttatcgacat ccagaataat
    10201 tactctggtt tccacaaaac tctccttgtc attgccttta ttctttgtct tgcttggaat
    10261 aaatgattgt gatgaattta gtatctctac aaactcatct cttgtaactt ctctatagtt
    10321 atctaaacac ctatctctta catcagtttt ttcttttttt gtaactggct tctctaataa
    10381 tttttcttga tctacataaa ttggaatatt aatcattgat gtttccattt gtgtttcctc
    10441 cttataaaat tctgttaaag actaaaacat acagataata aaaacaaact ctttattact
    10501 atatgtttta gtcttatttg tttactcaac ctttataccg ttccatgaaa ctatcaatat
    10561 cagatttttt aaatctaatg accgtatcaa tttttataac aggtaacccg tattgagaaa
    10621 catattttct aatcgttcct actgatacac ctagatattc acttgcctgc tgatagtcca
    10681 tatactctag agtgactcca gaaataatgt cttctagcat aggctttagc actgtaatta
    10741 tctctttcac ttgttgttca acttgtatag aaacaatgct atcaatgatt tgtagagaca
    10801 tatttccccc tcctttcgtg attgcttgaa caaattttct aagaattatc ttcgtatttt
    10861 ttgctcaaaa taaaagtatt ttgttattaa ccgattaatc aaacaaaaaa tgattaccag
    10921 ttcatcgaca tacacataat acctcgataa aaaaggggtg tcaacagctt tgtaaaattt
    10981 atttacaaca cccctttttt tgaggtatac taattttagg aggtgtggtc aatatgataa
    11041 aacgtccacc aataaattat ttagaacgaa aaaagatact cggaacaaaa ataaaagcta
    11101 taagaaagag taaaaaatta acacaaccag catttggtct tatgattaac aacggtcaat
    11161 taattgacaa aaaaactatc tacgaatggg agaagggaac ctatttacca ataccagagc
    11221 gattatcccg aattgctgat ttgggaaata tgagtataga ggagttagtt tgtggcaatg
    11281 tagaagaata tattttggga attattcttt atagagatag cattgtacta gatggtataa
    11341 catttccaga taaaaacctg ttccagcatc ttcgtcaaca gttcccacca gtacactcta
    11401 atttagatac atggctggat agatattcca aattagaacc agagatgcaa gaatttattg
    11461 ctaacaagac ttgtaataaa gttaaaaatg aaaaaattag tcttttcaat atcttaaaaa
    11521 tagaagaatt attcatcaat gctattgtag aagaatttga caataacatt cttttcctca
    11581 ctagcagtat agaagaacta ctagaaagaa tggtagatga atggctacct attcaattga
    11641 aagacatgtc ttatcccgaa gaagctgtga gagaaattac tgataatata aacaagctag
    11701 aacaaacgat ttcatcaatt gggaaaaaat acactaagaa aaaaatgaag ggaggtgata
    11761 ccatctagtg gtaagtactc cttgtaaact aagaggagat gatttaatgg caaaaatatc
    11821 aaagtacaaa aagaaaaacg gtcaagtggc gtggatgtat aaaggacaca ttgccaccga
    11881 tccacgaact ggtgaaaaaa taaatactac gagaagaggt tttagtagca aacaagaagc
    11941 tcaaaaagat tatgacgagt acagacatca aatattatac ggtgttaaaa agaaagcacc
    12001 tgatatgaca tttgaagacc tgtataatga atggataact catcagcgta ccagtgttaa
    12061 agcttcaaca atcgcaatct cagtaagata cgcaaataat caaatacttc cagcatttgg
    12121 taagcttaaa ctatcgaata tctctgtccc atattgtcaa aaagtggtag atgaatggca
    12181 cagtaaatat gaaagttacg attatatgag aaaacagact gcacaaattt tacgctacgg
    12241 ggttgcaatg caatatattg acaataatcc tatggaaaaa acacttcttc cacgaaaaaa
    12301 agagtatgag aaaaatcgta aattttactc aaaagaggag cttaataatt tactggatgc
    12361 ttttaaagat tttggtaaca tgaaacaata cgccttcttc agattattgg catacacagg
    12421 tatgagaaag agtgaagtac tcgctttaca gtggaaagac atagacacgt ttaataaaga
    12481 actacatgtc aataaaactc ttgctgtaga tgaatttggt aaagttataa ttcaatctcc
    12541 gaaaacaaga gcttctagac gagttatctc tttagatact gagactcttt ctatcctcaa
    12601 caactggaaa ttacagcaaa aagaagagta tttaaaactt ggatacaata ctagctctaa
    12661 agagcaacat gtttttacaa cagttaaaaa cactctatac attccaaaca ctgttaatga
    12721 ttggttaaga tatattttga aaaaatataa cttgcctcgt attacacctc atgggtttag
    12781 acatacacat gctagtttat tattagaagc aagagaatct gtcaaggtag ttcaacaacg
    12841 attagggcat gaaaatagta aagtgacatt agacatctat gcacatatta caaataatgc
    12901 acctaagaaa acaggtcaag actttgctga tatgatggct catcagtaaa agagctggtc
    12961 aaaatctggt catttctagt atacaggcaa aacaaaaaag ctagaaccct tatatatcaa
    13021 gggttctagc tcctataaca ggtttaccca acactgcctt ccatctcata actgatcaat
    13081 cggttgagtt cgactgcata ttccattgga agttctttgg tgaatggttc aacaaagccc
    13141 atgacgatca tttcagttgc ttcagattcg gtcaggcctc ggctcatcag atagtagagt
    13201 tgttcttctg agatttttga gactttggct tcgtgttcta aagagacttg gctgttgtgg
    13261 atctcattaa atgggatcgt atcggatttc gaccattcgt ccatgatgat tgtgtcacat
    13321 tcgatatgag agatcgaacc cgcactgttt ttggcgaagg ttacttgtcc tcgatagttt
    13381 acttctccgc cgtcttttga gattgattta gagacgatag agctagatgt atttggtgca
    13441 ttgtggatca tttttgcacc cgtatcttgg atttggtttg ctcctgcaaa ggcgatggag
    13501 agcatggtgc ctcgtgcgcc ttttccatct aagtaaacac ttgggtattt catcgttgtt
    13561 tttgcaccta ggtttccgtc aatccattca acggttgcgc cttcgtatgc ttttgctcgt
    13621 ttggttacta agttgtacac attgtctgac cagttttgga tcgttgtgta acggcaatac
    13681 gcatcttttc ttgtaaagat ttctacgatg gcggcatgca gactattact tgaataagta
    13741 ggtgccgtac aaccttcgac atagtggatg cttgctcctt cgtcaacgat gatcagcgta
    13801 cgttcgaatt gtcccgtgtt ttccgcattg atccggaagt aagtttgtaa tggaacatcg
    13861 actttgacgc cttttggcac gtaaataaag gtgccacctg accatacggc tgaattcagc
    13921 gctgctaatt tgttgtcagt tggcggtaca agttttgaaa agtactcttt gaataattca
    13981 gggtattctt tcaaagcaga gtcagtgtca gtaaagacga ttcctaactt ttcgaattca
    14041 tctttcatat tatggtacac gacttctgat tcatattgtg cggaagcacc ggctagataa
    14101 gctcgttctg cttctgggat accaatcttt tcaaatgtat ctttgatttt ttctggtacg
    14161 tcttcccagt cacgagcggg tttgtcactt ggtttttggt aatatttgat ggcatcgaaa
    14221 tcgatgtctg aaaggtcggg gccccatgtt tgcattggca ttttgttaaa tgtttctaga
    14281 gatttcagac ggaattcaag catccattct ggttcgcctt tgattcttga catctcacgg
    14341 atcacttctt ctgtcaagcc ttctcctgta ctgaaaaccg gttcgacatc atcgtgaaac
    14401 ccgaatttat attcttctaa ttctggtacg cccatgttct cactcctctt ccttgtggta
    14461 atgatggaaa gctgcggtac catcgttttc cattgctttt cctagtgctt tccaggcaag
    14521 tgtcgcacat ttgatccgag ctggaaattt agctacacca ccaagcatag cagcatcgcc
    14581 taattggtcg ggatctttga cttcttttcc ttggacaagc tgtagaaagt ctacagttaa
    14641 atgttctgct tcttctattg attttccgat gacggcgtct gtcatcatcg aggcacttgc
    14701 cgtactgatg gagcagccac tgccgtcaaa ggcgatattt ttgatgattc cgtcttttac
    14761 atcgacttgc aattcgatca catctccaca tgtggggttg ttcaattcga tcttacttgt
    14821 cgagtcagtc aagcttccac gatgatgcgg atgggaagaa tgatccaaga tcacttgacg
    14881 gtataaattg tctaatttag atagtcccat gctggaaaaa ctcctttgtt gccatgattg
    14941 cttcaactaa acggtcggcg tcttgcgttg tgttgtagaa ataaaagctc gcgcgagcag
    15001 tcgctgatac ttcaaggtac ttcaatagcg gttgcgcaca atgatgacct gctctgacgg
    15061 ctaccccttc catgtctaaa gctgtcgcga catcatgggg atgtaaccca tccaaattga
    15121 atgcaacgac tcctgtccga aaattcgggt cttgtggacc ataaacagtc aatccatcaa
    15181 ttgcttgtaa ttttggcaac acataagcgg ctagttcttc ttcatatcga tggatattgt
    15241 ccatgccgag ttcttgcaaa taatcgattg catggcctaa tgcgatagcg ccggcgatat
    15301 ttggtgtccc tgcttcgaat ttccaaggta gttctttcca tgtactgtcg tataattcga
    15361 caaaatcgat catttcgcca ccaaattcaa caggttccat ttcttctaac cattttcgtt
    15421 taccatataa agcaccaata cctgttggtc cgcacatttt gtgtccgctg aaagcaaaga
    15481 aatcacagtc tagtgcttgg acatcaatgg ccctgtgcgg ggcagattga gcaccatcta
    15541 ctacgatgac cccgccgttt tcatggacaa gttcagctaa ttcttgaaca ggattgatga
    15601 cacctaagac attggataca tgagcaatgg agacgatttt ggttttgtcc gtaatctttt
    15661 tcgctgcatc ctccatatcc aggaagcctt cttctgttag ttcgatatat ttcaagacag
    15721 cacctgttcg ttttgccaac tgctgccacg ggataacatt agaatgatgt tccatgtaag
    15781 agagcactat ctcgtctcct gctttgatat agcggtcgcc gaagcttctg gctacccaat
    15841 tcaagcttgt cgtcgtacct cgagtaaaca gcacttctgc tgtttctttt gcatggatga
    15901 acgcccgtac tttttctctt gcttgttcat aatcatgggt cgcgcgttct gctaacgtat
    15961 ggacaccgcg atgaacattg gcgttatcgt gttcgtaata gtgcgctaat gcctggagta
    16021 cttgttttgg cttttgggtc gtcgctgcat tatccagata aactaaaggt tcatcattga
    16081 cgtcttgaaa taagatagga aaatcattgc gtagcttttt agcatcgatc atccattcaa
    16141 cttcccttcg attacttcga ctaattcttg ccgaacttgt tctacaggga ttgctgttaa
    16201 gacagaaccg aggaatcctc gaatcactaa gcgctctgct tcttctttat gcaatcctcg
    16261 actcatcaga tagtacattt cttctggatc aactcgtcct acactggcag catgtccagc
    16321 agttacttcg ttttcatcga tcaaaagaat aggattcgca tcaccacgtg ctttatcaga
    16381 aagcatcaag acacgacttt cttgttgggc atctgcacct tttgcccctt tcaagatgtg
    16441 gccgatacca ttaaaggtca atgttccttt ttctcggatc actccgtgtt gcaaaatatg
    16501 cccgatcgta tgaggggctt tattggttac tcgcgtatcg attccttgga tttgtctgcc
    16561 cgagctgatt gccacgattt tgacttctgc gtgtgcacct tctcctgcta aatctgagtc
    16621 aaagtcagcg atcacatgac cgtcattcat gacaccaatt gcccagtcga cagaagcatc
    16681 tcgtaaaatg tgcccacggc gattcatata agaagtaata ttttttccta attgatctac
    16741 ggttgaatat ttgatttttg aaccagcttt agcgatgact tccacgatga tattacctga
    16801 tgattttgct acttgttctt tggttgtctg gaaacgttcc agataagaaa attcactatg
    16861 ttcatctgcc acgatcaaca catgcttgaa gaagtgctca tcggatgcgc catcttgaat
    16921 gaataaggac tcgagtggtt catcgatcac gacattttta ggcacataca ggaaaatacc
    16981 gctgttcata aaagctacat ggagtgcagt caattggtct tcgttcatgt ttactgcttt
    17041 tttcatataa tattcttgga ctagttctgg atattcgatc attgcagtga acaagtcggt
    17101 aaaaatgact cctttttctg ctaattccac aggcagttgt tcaaaaatcg tccatgaacc
    17161 ttgttggaca agcacaggat tgtctttcat tgcatcaaat gccggaatcg tgcctgtttc
    17221 actgatggtt tgtgtttctt tcacatcaaa taacgaccag cgatggaatt tcacccgatc
    17281 gatatgcggc aatggcagtt catctgcttt cgctagtgcg tcttgacgga aggttctcat
    17341 ccaatcaggt tctcctttac ttaaagaaaa atcatttacc gcgtcaagat aatcgagcca
    17401 gtttttttct ttcattcttg ctccccctta cgcttcttct tctttatagt cgattcctaa
    17461 ttctttactg ataccagcgt agccttctgc ttccagacgt ttagctaggt cagcattccc
    17521 tgtcatgact actcggcctt ccatcatgat gtgaacgaca tcaggtgtga tatagttcaa
    17581 taatcgttga tagtgggtga tgatcaatgc accgaaattg tctccgcgca tttcatttac
    17641 tcctttagca acgactttca aagcatcgat atccagacct gaatcgattt cgtctaaaat
    17701 tgcaaatgtt ggttctaaca tcaacaattg caggatctca ttccgtttct tttctccgcc
    17761 agaaaaacct tcgttcagat aacgttcagc catttcttct ggcatattta gtaaagccat
    17821 tttttcatct aactttttca aaaatgccat aacagaaatt ttgtcgtttt catcacgttt
    17881 cgcattgatc gctgcacgca tgaattccgc attcgtgata cctggtatct cacttggata
    17941 ttgcatagct aaaaataagc caagtcgcgc acgttcatct acttccaagt ctaaaacatt
    18001 tttcccgtta aacaaaattt cgccttctgt tacttcatag tttggattgc ccatgatagc
    18061 ggcagataag gtagattttc ctgtgccgtt aggtcccatg atggcatgga tttctccggt
    18121 tttcattgtt aggtttacgc ctttaaggat ttctttttcc tcgatcgaca catgtagatt
    18181 ttttatttct aagacagaca tgcattatac ccccaaattc tctttcgttc tcactcattt
    18241 tagactaatt gagaatgaag cgcaatgacc ttccttacta aaatcaaata tcttttattt
    18301 attattaaac aattttgatt tctaaattag aatagatata aaaaa
//
LOCUS       DACDYJ010000054        17980 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDYJ010000054 DACDYJ010000000
VERSION     DACDYJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.054"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            100..2427
                     /locus_tag="IWM51_001933"
     CDS             100..2427
                     /locus_tag="IWM51_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9971345.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2553..2882
                     /locus_tag="IWM51_001934"
     CDS             2553..2882
                     /locus_tag="IWM51_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9971346.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2922..3926
                     /locus_tag="IWM51_001935"
     CDS             2922..3926
                     /locus_tag="IWM51_001935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9971347.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3923..5352
                     /locus_tag="IWM51_001936"
                     /pseudo
     CDS             3923..5352
                     /locus_tag="IWM51_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5558..6731
                     /locus_tag="IWM51_001937"
                     /pseudo
     CDS             5558..6731
                     /locus_tag="IWM51_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
     gene            complement(6893..7348)
                     /locus_tag="IWM51_001938"
     CDS             complement(6893..7348)
                     /locus_tag="IWM51_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971348.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            7937..8359
                     /locus_tag="IWM51_001939"
     CDS             7937..8359
                     /locus_tag="IWM51_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9971349.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(8525..9718)
                     /locus_tag="IWM51_001940"
     CDS             complement(8525..9718)
                     /locus_tag="IWM51_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9971350.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            9942..10319
                     /locus_tag="IWM51_001941"
     CDS             9942..10319
                     /locus_tag="IWM51_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9971351.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            10307..10645
                     /locus_tag="IWM51_001942"
     CDS             10307..10645
                     /locus_tag="IWM51_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9971352.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            10779..11222
                     /locus_tag="IWM51_001943"
     CDS             10779..11222
                     /locus_tag="IWM51_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP9971353.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            11484..12299
                     /locus_tag="IWM51_001944"
     CDS             11484..12299
                     /locus_tag="IWM51_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9971354.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            12309..12971
                     /locus_tag="IWM51_001945"
     CDS             12309..12971
                     /locus_tag="IWM51_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9971355.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            13176..14840
                     /gene="alsS"
                     /locus_tag="IWM51_001946"
     CDS             13176..14840
                     /gene="alsS"
                     /locus_tag="IWM51_001946"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP9971356.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            14853..15563
                     /gene="budA"
                     /locus_tag="IWM51_001947"
     CDS             14853..15563
                     /gene="budA"
                     /locus_tag="IWM51_001947"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP9971357.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            15693..16190
                     /locus_tag="IWM51_001948"
     CDS             15693..16190
                     /locus_tag="IWM51_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP9971358.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            16375..16635
                     /locus_tag="IWM51_001949"
     CDS             16375..16635
                     /locus_tag="IWM51_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971359.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(16997..17236)
                     /locus_tag="IWM51_001950"
     CDS             complement(16997..17236)
                     /locus_tag="IWM51_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971360.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            17522..17836
                     /locus_tag="IWM51_001951"
     CDS             17522..17836
                     /locus_tag="IWM51_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971361.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT         6097 a         3103 c         3540 g         5240 t
ORIGIN      
        1 aaaaaagccc caaactttct atacatcaat aaaataataa aaattaaatt tagaataacg
       61 tgtaaaggat tatttgatga ttttttattg agaggaggga tgaaaatgat taagaaaact
      121 caaaataaaa tccaacgtat atgtcaaaaa cattttgttt ctagactcaa agctgtatct
      181 aaaagaaaat acttacatcc cattatgatt tcgatcatct tgatttttac tttcttaggg
      241 agcttatcaa gtatttatgt aaacgtttat acagaaacgt caaattttgc taaccaaacg
      301 acagaggaga caaatagtcc accctattct gaaatacagt ccatagaaag caccaatcag
      361 tctagcaaca acgagacaac ctcaaccaca tcctcgactc aactgattga tccgacagaa
      421 aaagaaatca cagcagcaaa agcagcaggg gagaaagctt attcagaaac aggtagaagc
      481 cagacattta gtactgttgc ggccactgcg aatttcaacg gacataccat accagatgga
      541 tatacagtga tatatgccaa agatggagtc attgttgcgg ttaacggatc gatgagtgcg
      601 gataaaactg cgttacaagg cgtaaccaaa gcaatcgctt ttattgataa ccgtccagat
      661 atccataatc tgtttattgg ctccacagta aatggtacta ctatcacatt cccaaaaagt
      721 atctatatgg gggtggctac cgctggaaat acgatcgatt cagttacacc caatgcggat
      781 aaaagttatt cttttacacc ctcaacatgg ggagggcttc ctgccggagg aggtatgagt
      841 actttttcaa tcggtataca gttagcaaat gatattccat tcacggggta ctcgatcaaa
      901 aatgggcaga tcgttgatgg taagggaaat gtttcaaatt ttccaaccta tcaagcagca
      961 acggttcagg tcaatgcgaa tcggacagga actttttctt atacagtcaa tggtggagca
     1021 gcaaaaatcg gaacgtacgg gactacatta tctggaatcc atgtgggtga caaagtggtt
     1081 atcactccaa cagcagtaac gggctattct tttgcacaaa caaacggttc tttcacagcc
     1141 ctagaaagta acgtcgatac ggtgacctat acaggcaaac aaacaccaat cactattaag
     1201 tatcaagact attttggaca gatgattgct ccttctaaaa taatgaatct tacttacgga
     1261 agtgctgcac aagacctgac gactaatacg cctactatca acggatatac ctttacagct
     1321 gtttcagcga cagaaacaaa gaaccaatca gcaatgagtg ttttagctag tttagataca
     1381 aatggaaatg taatcgtaaa ggacgcaaat ggaaaacaga tttctgaagt catcctatat
     1441 tataagacaa atgcaacagt atcaatcaat gcaaatggca gtaagtacta tgatggtctt
     1501 tctgttgtac cgatcgttaa atatgccttt aatgataaaa cagaatcaac atccctttta
     1561 aacaatgatc tcagtattga tcaaatcaca tggaatccaa ctgatttcaa agcgatgaat
     1621 gaaaagggag ttgaagtaac tgcgcctact gaaattggaa cctatacgtc ttggcaattg
     1681 acaaaaagcg gactagctaa actagctgcc agaacaaact atttatttac tatcgtgcag
     1741 acatctgatg tctatacgat taagcaaatc agcggtgcgg tagagctcag tggcagtaag
     1801 acatatgatg gaaaagcagg tgtcccgtca atccatgtca agctagcaga gggtgtcact
     1861 agttcgctta ctccagtaca agtggcactt agcagtatgg attacacagc agatgcacaa
     1921 tcagcaaaaa atatggttaa tgctggcagc tatacaatta aactaacgaa tagcggaata
     1981 gacaaagtca aagcagcaaa tcccaagttg tcatttactg atatagccaa cacatcagga
     2041 acttatacca tctataaagc taacgcagtg attactgtcg atgacgctgc ctttaattat
     2101 gatgcccaaa gtcactcgat ttcggttgga aatctccacg ttaccggtgt tgtcttaggc
     2161 gaatcattgg attataccct aacagttaac agccggacag atattggatc acaaatagta
     2221 acgattagtt taggcgaagg aagtgtaaac aataattata cgattatcgc aacagatacc
     2281 gctaagttga cgatcaatcc gactctcttg cttccttcga caggtgggat aggtctactg
     2341 ccctttgtct ttttaggact gatctttatt ttttctggct tcttttattt tatccatcgt
     2401 aagaaaaaag caggtgaaca gcgatgaaaa aaacaaaaaa tgtacgtgtg gatatatttg
     2461 ctgttctctt ttttatccta ctggtctttt ttataggttc tccgactgtg tcagctagag
     2521 aatcacctat tacatttact ttgcataatc gaatgttcga aagcgataag atgccaagta
     2581 tcagtcaaaa cgacggaata aatatcccgg ataactttca aggagtaaaa gggttgaacg
     2641 gcgttacttt cgctatatat gatgtctcgg atgaatttta caagctgcgg tcagaaggaa
     2701 gtagtgtgga agacgcacaa agaaaactag cacaaaaaag tgatagtgaa aaaatactgg
     2761 cagaaaatgt gacaaaaaca gtagacgagg aagagggcat tgcttctttt agtgcatcgg
     2821 acaaagatga gcaaggtcgt gacgctgttt accgatttgc ggaaataaag acttcagatt
     2881 aagtaaaaga aaaatcagca ccatttgtcg ttgtgcttcc tgtgacagat tcatctaatc
     2941 ataaattgac gaccatccac ttgtatccaa agagcgaaca aaaaatacct gctgcactca
     3001 cattaacaaa aacagtagag aataagcaga cagattttgc agatggggat aaagttcctt
     3061 atctcatcac cgtaacaatt cctgagaaca tcaacgagat aggaacctat acgatcaaag
     3121 atacagctga tcctcagtta tggctagaac cagaaacgat cgattttttg attggtggag
     3181 acaagattca tacatttcac acacaaaaaa caaaacatgg ttttgtctta tcagattctg
     3241 gaaaagatct tagttcgttt gctgggaaaa aattaacgat ccgttaccaa atgacattga
     3301 aagaaaacag tgaaaaaacg acatttgata acgatgtacg tttgacgact gacaatcaaa
     3361 cagtagaaac acatcttgcc atccaaacag gaggcaaaca atttgtcaaa gtggatcggc
     3421 aaacgaaaca aaaactagca gatgctcaat ttctagtcaa aaaaaatgac caatacttag
     3481 cgcaagaaaa cgggataaat cattgggtag ccaaagatga ttcaagagca acgattttta
     3541 cttccagtaa agatggaaca ttttctgtcc atggattgtc ttttggaagt tacgaactag
     3601 tggagattaa accgcctaaa ggatatatat tgagtcaatc aaccattctc tttgaagttg
     3661 aaaaaggcag ttatgatccg cttcatccta taccattgga aatcatcaac gaaccaaaag
     3721 caaatccgac gaataatgga aaaacgccag ttcagatgca gacaaatggt ccatcaaatg
     3781 aaaaaaatgg aggaagcttt ccaaaaacaa gcgaagagat gcttggaggt ttctcaattt
     3841 tagggttgtt gctagtcctt tcaacaggga cagcttggtt ttataaaaaa caacaaaaag
     3901 gaaacaatag gagggaaata aaatgaaaaa catcaaaaca aaaatatgga cgtttttagg
     3961 aactgctatc atgctgcttc ctttcgttct cggcctagga actgcagagg tttctaggcc
     4021 gtatcaccta caccagaaaa tgttacggtt aatttgcata aattgaaatt cacttcagca
     4081 ccagaaaatc aaataaacaa cggaacggaa ttgacatttc cgaatagcga accgttgaac
     4141 ggcgttgaat tcaacgtata tgacatcact gctacgtact atccaagtaa agacacagca
     4201 gttccagcag acgctacacc cttcgcaagt gtaacgactt ctggtgaagg attagctaac
     4261 ctgactcttc caggtaaatc tgatgggaaa gatgctgtat acgtctttgt agaaacacca
     4321 aagcccggtg tcgaaacgtc gccaaatatc gtgttaagct taccctttta taacccgaat
     4381 tcatcagacc ctgacaaacc tttagacacg attcacctat atgctaaaaa tgtcatcaaa
     4441 gaaagcaata tcaaaatcca gaaagctggg aacttttcag gggtagataa gttagcaggg
     4501 gcgcaattcc agatcaaaaa ttcagagaat caattcgtga ccggttttga tgaaaacact
     4561 ggtgtcgctc tctatgatcc tgctgagtca gcgttgacat tcacaactga tactgaagga
     4621 tccatcagtg tcaatggatt aatagacggc aattatactt tagttgaaac aaaagcacca
     4681 gatggttatc aattaaccaa tccacaaaaa gaaactactt ttatagtaaa aaacggaaaa
     4741 ttgcaacaaa caaccggtat catccatggc gcagatgaag ccaatcttgg gaatccaaac
     4801 ggtgtagtca atcgggaaaa tacaactacg gataacacga ttactgtaga aaatgtccaa
     4861 aactacggtg atttccactt catcaaacaa gatgtgaaca cgcaagaaaa attagccgat
     4921 gcagaatttt ctattctaag agcaaaagat ccaactgtta aattaatgaa aaaaatagca
     4981 aatcttgcct caggagaata cccatatgct tggtcagatg aagtcacagg ttctgactgg
     5041 gaagaagtga aattgatttc tgattcagaa gggaaatttt caatcgaagg gctgaaacaa
     5101 ggggcatact gccttcaaga aacaaaagca ccagaaggct acgcattacc taagaatgaa
     5161 gctgcgtaca ctgaatttac agtagatgat gatccaacaa cttctgatga aacaacgatc
     5221 gataaccaac ctaaaggaat cttgccttca actggtggta tggggatcat cgtattcatc
     5281 ttggtaggta ctgctttagt tggaggagca gtgatctact tcaaaaaacg ccatcaagaa
     5341 acagaagctt aaactattgg aaacaaaaat catacgataa ataaacgata gtgattcaaa
     5401 tggggagaaa ggttgtgaca agtggtcggt ctcttggtat tcgctcaaca aacggcaaaa
     5461 taggaggaac tattttgccg tttgttgagc gaatactcag gtcaaggcgc tgatttcaca
     5521 acctcttcat atacatatta gaaaataggt ggacatgatg aataaaagca gaataagaat
     5581 actggacatc ttcatggcaa tcattttagt cgttgggatc gggatctttt cttatccttt
     5641 tgtagaagat tcactgaatg attttcttgc ccagcaaatg atcatccatt atcaaaagca
     5701 agcatcgaat aagaattcag aggaaataaa aaaaacagca ggaaaaaatg acaaagaaaa
     5761 accaacagtt ggcagagcaa aacgtatcac cagggatagg cagtttcaac caagcagttg
     5821 atgcaaaagc attaaaagat ttacctagta atgccttttt tatggaacat atgcttggag
     5881 tcatcgaaat cccaaaaatc aatgtcagct tacctatatt cgatcagaca acggaaatat
     5941 ttttgcaaaa aggaacgtca ttattggaag aaagttctta tccgactggc gggaaaaaca
     6001 cccatgccgt tctttctggg catcgtggac tcccagaagc aaaattattt acagatttgc
     6061 ctaagttgaa aaaaggagac cagtttttca tccagatcaa tggaaagaca ttggcgtatc
     6121 aagtagaaaa aatccaagtg gtcttacctg atgaagtaga ttctttgggt atccaaaaag
     6181 gcagagatct cgtgactttg cttacctgta ctccttatat ggtcaataca catcgactgc
     6241 ttgtgacggg gcatcgtatt ccttatcaag caaaagaggc aaaaaaagcg atccaaggaa
     6301 ttgatcaatg gaaaaaatgg aaatttttta tttggtttat cggtatacta cttggatcaa
     6361 tcggcttagt ttggctactc atcgcttatt tagattcttt ggccatcgct aagcgaaact
     6421 atcctttatc tttttatgta aagaacacaa acggacgacc aatcgaagga atggtctttt
     6481 ctgtaaaaac gctcaacggc aaacactata ttactagaga aaaagttcca tttgtgaaag
     6541 cttctgatga atacggacta gtgagatttt ctgatttaaa aggcaggaac tatcgcttgc
     6601 aacatgagga gctcctgctt aaaatccatg taaaacataa acattcgaaa cagttttcaa
     6661 tgaaattgaa aaaaggaaga tataaactgc gaaaagaaaa agaggtgtac tatttgattg
     6721 agaaagaatg aatgaagatg tccaaaaaag cagagggaac agactaaatg aaaaaagaga
     6781 gaagctgacg aaatggacgt gtaaactgct atttttggtt tactatctgg aacgccagct
     6841 tctatttttt tatgtttcag aagaatttta ttattatagt ataacttttg aattagtttc
     6901 cataacattt ggagcaaggg gttaaaccac gagcttgagc ttcgcttaaa gtggcagggg
     6961 tatacgtacc atttccacat ttatgtgtat ggtattttga accggtcggt gtgacataga
     7021 cagtttgttc attattttgc tcaggagctg cggcttgtgc ctgctgagag gcctgttcag
     7081 ctgctgcttg ctgtcgcgcc tgttctgctt gttgctgtgc gattgcttct tgttgcgctt
     7141 gttctgcttg ttgtcgtgca atttcttctt gctttttccg ttcttcttct gcttcttttg
     7201 ctttgattgt ggaatcaatt gcagcaagac ggcttgtcaa ctcttgtttg ttttcaggta
     7261 gcgcttggat agctgctgaa gcagttgcat agttttcttt tgtaggattt gcttcagctt
     7321 gtgacaaagc agattccgct tctgtcactt ttttttctag tgcagctttt tccgcttttt
     7381 gtttctcttc ttcttttttc ttttcaattt gttcttgttc tttcgatgaa tctgccgtac
     7441 tttcggagat cgtggtactg ctgctggaag cctgagctga acttgtgctt tgagtagtag
     7501 acgtagtagc accacaccca ctcaacaaac ttcctgcgac caaaaacaat gcaactaatt
     7561 ttttcactaa atttcctcct taagtaaaaa acaaccgttt tcaataaatg tccttttcaa
     7621 ttgtttactt acttgttata ataattgtgt tatgaatagt gattgaggaa acttaatcta
     7681 atcccgtttt gatacacggg attttttgct atagttctgt tattaagtat gcaataataa
     7741 aatatgtacg ttattttatt attagattat tgacatttat ataataaacg attaaatgat
     7801 tcatatcaat agaatactta ggaaaaaaag agaaataata acaggtttat gcactattga
     7861 cggtgtgaca atatgatatt tgtatccgtt tttttcttgt atgtagtata gtaaagtaga
     7921 tacaggagga gaaaatatgc agactttttt aacaaaagtt tctcaaaaga aaattaaaat
     7981 gattcattta ctgcttgaaa cagaacgttg gtgttcaata gaagaactac aaaacgaatt
     8041 gaaggtatca agtaagtcta tcttgaatta tctaacagat ttagaagaat tgttccaaaa
     8101 atatcctgat aaggtcattt tgaagaatga gaataatcgg cgcttttcta tggaaaagca
     8161 ggagaacttc ccaatctaca ttatttatct tcatttctac agaaaatcat ataattatca
     8221 tttgattgaa ttcatgtatc aacatcctga gaaaaatttg gaagattatg cagacgctca
     8281 atatacgagt gtatccacag tttttcgtta tgctaagtta ttagtaaagt actttgaaat
     8341 gacggacgtc aaagaatagt agccgccaga tagaaggaaa ttagcagttg aaaaagctgc
     8401 cctgcatcaa gtaatacgac cagccaaacg gaaacagtgt ctcatgtttt tgatttggct
     8461 ggtcttatta ccgcagatgc aagcttttgc cacacctctt tcttgtcatt tacagactca
     8521 actatcaatg ctgcaagctt tcccttaatg ctgtaacacc agtaagagtt gtcaagacca
     8581 gcagactgac taggtaaata ccgctcaaaa acaagattac ggcagttaaa ctataatgat
     8641 ccatcaagaa accgatgacg atagaagaaa aaccaccaac agctcgtcca aggttcataa
     8701 tgaaattatt cgccgtcgcg cggatttctg ttggatacaa actactaatc actgcgccat
     8761 atccaccata catgccattg ataaagtagc cgactatcac agcggctgct accaaactcc
     8821 actggctatg ggctaacaca agtagataga cagataagga tgaacaaatt aaaaacaaag
     8881 aaaaggctag tttaggcccc aatttatcca tgattatgcc aaaagtcatc atccccaaag
     8941 acatgcctaa aatagtactg accatccaca atgaagaccc agaaaccgaa agccctaatc
     9001 gattttgcat aattgaaggc agccaattca ttaacccaaa atagccggca atttggacag
     9061 ttaccatgat actaaggcca catgtttgcc aagcaagatg ttttgattca aacagtctgc
     9121 taagtttggt tttcttttcg gcagattgta cagatttgaa ttcctttggt tcagtcaagc
     9181 cgcgacgaat gaacaagacc atcaacaccg gcagtacacc tacgacatac aacatcttcc
     9241 aaccaaacgt tggaatgatc acagcagcta aaatcgcagc caaagccgca ccgacctgcg
     9301 caccaatacc agcaaccgaa gaagctcgtc caatttgttt ttttgaaaaa ctttcggaaa
     9361 tcaaggacat acacgccccg tattctcctc ccgctcctac acctgcgata aagcgaaaga
     9421 gataaaccaa gtagatgttt gaagcaaaat acatcaataa agaagcaatc gaaaacagaa
     9481 ggaccgtttg agaaaatact ttcacacgtc catatttatc agctatgatg ccaaaaaaaa
     9541 tacctccaat gagcatcccc aaattagtga tcgttgaaat gaaacccgct tgcgtaccac
     9601 taatatgaaa agaagtaatc attgaagata atgaaaaagc taaaaacata atatccatat
     9661 tctctaaagc aattcccgcc gatgtcgata ataataactt tcgctgatac gttgtcattt
     9721 ttatacccct tttcctaaaa tttccctaaa ttgttaaaag tatatccaaa aaagaataaa
     9781 aaaacaagac ttttttattt gaaataatac acaaacaacc gatatagata atccagctta
     9841 gttttaatag aaatgagtat tgacttggag tacacttaaa gttgtagaat aaataaaata
     9901 cataagtaaa gtggcaagaa attaagagaa agagtgatca gatgaatatc aaacaagtca
     9961 gtgaagaaaa gggcatttcg gccgatacat tgcgctatta tgaacgaatc gggttgattc
    10021 caccagttaa tcggacaaaa ggcggaatcc gtgattatac agaagaagac ctaaaatggg
    10081 tcgactttac gatctgtatg cgaaatgccg gattatcgat cgagtctttg attgaatata
    10141 ttcgcctatc tagcgaagga gaagcaactg tatttgagcg gcgtaagctt ttgattgaag
    10201 aaagtgaact tttgacgaaa aaaattgctg aaatgcaaga atgtttggaa cgtttacaag
    10261 gaaaaatcaa gaaatatgat cgtgtgttat ctagaaataa aatggaatgt atcaagtaaa
    10321 aaaatcaagg agcatcgcta tggagttacg cttactaaaa tatttttgga caattgcaga
    10381 agaaggaaca atctctcaag ccgcagaagt tctacatctt acacaaccaa ccttaagccg
    10441 acaactgaag gagttagaag aggaactagg aacagaactg tttgtacgcg aaaaccgcaa
    10501 aatgatcttg acagaagcag gttattttct aaaaaagccg agcagaagaa attttagatt
    10561 taacgaatca aaccactcaa gaattcgaga acagaaaaaa acaattattc agcggccgat
    10621 ttacgatcgg ctgtgtggaa gctgataatt cgaatacatt ggcactatta ctagaaaagt
    10681 tgatccgtga ttatcccagc gttcaatttc atatccacag cggaacaagt gatgatatca
    10741 cggagtgact agaaaaaagc ctgttagatt tagcgatttt gctagatcca gtagatacag
    10801 agaaatacga aacgattttg ttaccaagaa aggaaaaatg gggattgctc attttagaaa
    10861 attctgtttt agcaaaaaaa gacgtgataa caaaagaaga ctttgcgaat gtacctcttt
    10921 tgatctcaaa gcgtccagaa atacagcgaa tgtttgctga ctggttaggc ataaaaaagg
    10981 agcagttgct gatcacagga acgtacaatt tgattttcaa tgtatttgcg ctaattgaga
    11041 atcaggtagg tgctgcttta gtcatagaag gtgctgtaag tcatctttca aagcgagtga
    11101 cattcgttcc gcttgaccca atgattgaaa cgaactgcat gctagtctgg aaaaagaatc
    11161 ggattttatc acctgtagtc aatgaattga ttcaccgttt caaagaagct tttgaacagt
    11221 aataagtatc tctaatcgaa gaaatttcaa tacaaaagcg agacgtatga cagtcttgct
    11281 tttgtatttt ttcattttta aatcaaaaaa aataaagaga tttacataac ttgttaaata
    11341 agcgcttaca aacataagaa gaacggaaat gaatcagcta taataaccgt tgttctggat
    11401 tgtaatcatt acaaagaaat gaagattata actttggagg tgttcgaaat gaaagaaaaa
    11461 aagcaaaaat tcgttcaaaa aacatgccct ttattcaatg gttgggtatg attctgctga
    11521 ttgtattagc tttattgacg atcattcctt ttttatggat ggtttctgct tcattcaaag
    11581 caaataatga agtgttcacg attccgattc aatggatccc taaaacttgg catttagaga
    11641 attatctggt tatttggtct cgtattcctt tagtgacatt ttttaagaat acagcttttt
    11701 taagcgttgt gatcacgttg attcagttgt ttacttctag ttttgctgct tacgggtttt
    11761 ctaaactaaa ttttaaaggg cgcgatattt tatttttagc atatatcggc acgattgctg
    11821 tcccttggca gtcttatatg atcccgcagt tcattatgat gagaaaagtg ggtttaacat
    11881 atacgttgtg gtcgttggtg ctattacaag cgttcaatgc ttttggtgtg ttcttgttga
    11941 agcaatacta cagtagtatc ccagattctt tatgtgagag tgcgcggatc gatggcttat
    12001 cagagtgggg aatctatcgc aagatcattt tacctcttac aaaaccggcg attgccagtt
    12061 tgacgatcat cacattcgtt aatacatgga acgattatat ggggccattc atttatttgt
    12121 cttctacgga aaataaaaca atccagttag ggttgaaaat gtttattggg ctatttgatg
    12181 cagagtacgc attaatcatg gctgcctcag ttgtttctgt tcttcctgtt gcttttatat
    12241 ttttagcgat gcaaaaatat tttgtagaag ggatcgcttc atcaggaatg aaggaatagg
    12301 aggtgattat gaaagagcag cgttttgaca aaaatgttta tatggaattc ttttattggt
    12361 tgtatcaatc attggcaagt agcctttgtt tttggtgtgt caatatcttg tatatagcag
    12421 ctgcgttcct tttgtcgata gatggaagga atgtcttttc ctttattttt tctttacttt
    12481 ttattggtcc aggttgcatc gtgatgatag gtatgacaga agaattagca aaagaaaaaa
    12541 gtttttcacc agtacgtttg ttcataaaga cgtttcgcca attttggcta aagggatttt
    12601 tttattggct atttgcttgg atagttagtg tgataatgat ttttgattgt ttctttttta
    12661 tacgtttttc ttatggaaaa tggttgattc cgttgtttgt tttacttgct tgtttatcag
    12721 tttcctttag catcaactac tggtattttc aggtgagaaa tccagcgtcg aaaccaaatc
    12781 aagttctacg gatagcgttc tactatacgt tgaaaaaatg gtatgtgagt ttactagatt
    12841 ttttactatt gacaagtttg tttctcttct ttttcgtaaa gccccaatgg tgtattctac
    12901 taggtccttc tatcgtattt ggtttgattt acttcaacaa tcgtaaattg atgagaacta
    12961 tggatctgta gactttataa attgaaactt tcaattaaga atgacagttt ttatgtaaaa
    13021 actgtcattc tttttcattt tttgaaacat atggctaaac aaatgaaata aaacgtgcta
    13081 tacttttgaa tgaaacaggt ttagaaactg tatcaattat ttgtgattat ttctaactaa
    13141 taattaaaaa taaagcataa aaaggacgat gttcaatgga cgaaaaaatg aaacaaggtt
    13201 cgaaatctgt agtagaaagt ttgattaatc atcatgttga ttatgtattc ggtataccag
    13261 gtgcaaaaat cgatggtgta tttaatgaat tagaggatca aggtcctgaa ttaatcgtga
    13321 ctcgtcacga acaaaatgct gcatttatgg cacaagcaat cggacgtatt acaggagagc
    13381 caggagtagt tatcgcaaca agtggtcctg gtgctagtaa cttagctaca ggtttggtaa
    13441 cagcgacagc agaaggtgac ccagttttag caattgccgg acaagtaaaa cgaagcgatc
    13501 tattgaagtt gacccatcaa agcatggata atgcagcact gttccagcca atcacaaaat
    13561 acagtgcaga aatccaagat ccagaaacaa tttcagaagt gattgccaat gcttatcgta
    13621 tggctaagag ttcaaaaaaa ggcgcgagct ttatcagtat cccgcaagat gtagtggatg
    13681 cacctgtcca agggaatgtc atcaagccat tgagcgatcc taaattaggt tctgcttctg
    13741 cagatgatat ccgttaccta gctgaacgca ttagagaagc aaaactaccg gtactattag
    13801 taggtatgcg aggatcaagt gaaaaagaaa cgttagccat caggcaatta gttggaaaga
    13861 cagcattgcc tgttgtagag acattccaag cagctggagt gatctcacgt gaattagaag
    13921 cgcatttctt tggtcgtgtg ggtttattta gaaaccaacc aggagatatg cttttgaaac
    13981 gaagtgatct agttattgct attggctatg atccaatcga atatgaagca agaaactgga
    14041 atgcggaaaa agatgcgcgg atcattgtga tcgatgaagc gccagctgaa atcgatcctt
    14101 ttatgcagcc tgaaagagaa ttgatcggtg atatctcagc cactcttgat ttattgacag
    14161 gttcattaga gcctcaacaa gtgtcagaag atgcaaaaga atatttagca tctcttcaag
    14221 caaaattaac ggagcgggac attgttcaat caaaaggaga agcaggtatc ttgcatcctt
    14281 tagaagttat taacacgctt cagtcaaaag taacagatga tatgactgtt accgtagatg
    14341 taggtagtca ttatatttgg atggcacgcc actttagaag ttatgaacct cgtcatttgt
    14401 tattcagtaa tggtatgcaa acgcttggtg ttgcattgcc atgggcgatt tctgctgcat
    14461 tggtgcgtcc aaatacacag atcgtatcag tttccggcga tgggggattt ttattctctg
    14521 cccaggattt agaaacggcg gtacgtaaaa aattgaatat cgttcatttg atttggaatg
    14581 atggacacta caatatggta gagttccaag aaaaaatgaa atatcagcgg gcgtcaggtg
    14641 tagactttgg tccagttgat tttgtgaaat atgcagaagc ttttggcgct aaaggaatcc
    14701 gagctacaag tgtagaggaa ttagaaaaag ctttagaaga aggatttgcg acagaaggac
    14761 ccgtaattat tgatattccg attgattacc gtgataatga gaaattggga gaaacaatct
    14821 taccagatca attctattaa ggaagtgtcg acatgactga aaaaatctta taccagcacg
    14881 ggacattagg agctttgatg gccggcttga tggatggaac agaaacgatt gctcatatat
    14941 tagaaaaagg aactctggga ttgggaacat tgcatgggct tgatggtgaa gtcatctttt
    15001 tagatggcgt agcttatcaa ggaagatcag atggctcagt tgcccaatta gatggaagtg
    15061 aacttacccc ctatgcagca attacagatt ttacgccaga tactagtttt tctgtctcaa
    15121 aaactgcaga tggagaacaa ttgaaaaaag acatattagt aagagaagcc ggggaaaacc
    15181 tatttttggc agtgaagata actggattat tcaaaaatat gcacatccgt atcatgccga
    15241 agcaagaaaa accttacaga agactagcaa aaatttcaga gtctcaacca gaattccaac
    15301 aaagcaatgt ccaaggaaca ttagtcgggt tctttacgcc tgaattattc caaggagttg
    15361 ctgcagcggg atttcacctg cattttatcg atgatacaca tacatttggc ggacatgtaa
    15421 tggattttga agtggctgaa ggaaaagttg agattagccg catctcgtcc cttgtacagc
    15481 attttccagt tgatgatccg acttttgttc acgaaacgat tgattatgct gatttagcag
    15541 cagaaatcca acaagcagaa tagaaaaaga cagcgagatg cgttcaaggt gattaacctg
    15601 acgtatctcg tttttttgag gagaaaacct agcgtttaca aaaaaactga gctataatct
    15661 tctccataga tcaaaaagga gcggttttta tcatgtggac agaaaaacag aaaaaagatg
    15721 catacgaaat tttattatta cagcaacaag gattattaga agcagaagat aactggttag
    15781 caaatttagc aaattcatct gcattgttga atgagacttt gcctgagact gtctttgcag
    15841 gatattattt attcgatggt aatgaattga ttttaggacc gttccaaggg cgagtttctt
    15901 gcacacgtat caagatggga aaaggagttt gtggagaatc agctgaaaaa caagaaacat
    15961 tgatcgttga tgatgtcaaa acccatgcaa attacatttc ctgcgattca gccgcacaat
    16021 cagaaatcgt agtgccaatg gtaaaagaag gtaatttaat cggtgtttta gatatcgatt
    16081 gcggagtaac gaaagggtat gatgaaatcg atcaagcatt tctagaaaaa tatgtagatc
    16141 ttttattagc aaattttaat ataaatgctt tttcatttat taataactaa aatcgtctat
    16201 tgaaacaaaa aagaagagaa atcaagaata aaaagagccg gaaagacagt gggcatttct
    16261 gtttttcggt ttttttattt ttttataaaa tcacaagtgt tattgatttt cataatcata
    16321 ttttaatgtt attatcattt taaataatga aaaaacatta aggatggatg aaatatgaat
    16381 aacgaaacta tcattttagg aaaaaaatat atttgccaac ctattggctt gaaacaaaaa
    16441 gtgattggag aagtaatcag taaattagaa aattgtgttg tgatctgcgt agacgaatat
    16501 catgacgtcg atcgcaatga aatattagaa aaatgcggaa aagtagtggc aaaatatact
    16561 cacgtttatg agttggaaac aacagaatat ctatataact atagcaacaa atatgaacat
    16621 actttagttt cataaagatt taaataaagt gccttcaaaa gcgagaagac caagcttttg
    16681 aaggcacttt atttagttgg cagtgattgt tcatgatttt tgatttattc aaaaggttcg
    16741 cctagagaat ctgtctctgt aacgaggcct ggtggagaaa ctcgataaaa acctatactg
    16801 ccagaactgt cctattttcg gatagacaaa ctagtagttt taaaaagatc atccgatcca
    16861 atcatttcaa aaaatgcaaa actgatatca ggatcattta gctcaggctt gttatcgtaa
    16921 agcatttgta aaggttgcat ttagttagga gtaaacgcga aactatcatc attatttgct
    16981 gaagaagatg aagaagctaa ttcagagttt gtttgaaata aaggagacgt gctgacagtt
    17041 tctttaccct gttttttgca gaggtagaaa cttttatatc ggaagtttct accggagaat
    17101 ttttctcatt tgtagaaatt gttgaatcga tactattcga aatttgcgta tctgaaggta
    17161 tgttctttga tgcagaagtc ccacaagcag taagtaacaa taaatttgat aaactgataa
    17221 acagaattct tttcattatg tacacactcc ttatagtatc gtttagcata cacctatgct
    17281 cagtattagg caaaatcaat gcaaaactat caagtctttt atagtatcta ttagtcttag
    17341 taatacatat atggagaggg tgatatccca gttcaaacca gatacattgg ttaccatctg
    17401 tattaacgat tatgcctcta aacgcttttt ttatgaaaaa atttataagt gaaaacgtag
    17461 gtatgatatt atctagaggt aatttttttg ggtatttaaa tagttgctag gaggatagct
    17521 tttggatttt tacagtaatt ttatcttaat aatagcaata ttattacttc taaatatttg
    17581 gttttttgat aagagtagaa atgcagggat aggttttaga accaaacgaa gtacgtcttc
    17641 tgaaaaaaag tgggtatatt cacagactat tttttacgga ggggttattt caatcagttt
    17701 actttcttca acactttatt cttttaatgt tatagacgtt tccatgtcaa attttatatc
    17761 tattattgga atccttattt ctgcaattat tacacaacta cttcttgtgt ttgaagagaa
    17821 gtcaaaaaat aattaaagca attatgcctt ttagtacagg aattattact tcttacaaat
    17881 gtatagcgaa tgtgaagcct gctgttttaa ttgttctaga aaaacttttg agtgaacaat
    17941 tattttatgt taggatgaat taatttctct agagtttata
//
LOCUS       DACDYJ010000055        17833 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDYJ010000055 DACDYJ010000000
VERSION     DACDYJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.055"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            122..934
                     /locus_tag="IWM51_001952"
     CDS             122..934
                     /locus_tag="IWM51_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP9971362.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            931..1731
                     /locus_tag="IWM51_001953"
     CDS             931..1731
                     /locus_tag="IWM51_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP9971363.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1892..3160
                     /gene="rseP"
                     /locus_tag="IWM51_001954"
     CDS             1892..3160
                     /gene="rseP"
                     /locus_tag="IWM51_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP9971364.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3228..4937
                     /locus_tag="IWM51_001955"
     CDS             3228..4937
                     /locus_tag="IWM51_001955"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP9971365.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5149..9501
                     /locus_tag="IWM51_001956"
     CDS             5149..9501
                     /locus_tag="IWM51_001956"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP9971366.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9645..10118
                     /gene="rimP"
                     /locus_tag="IWM51_001957"
     CDS             9645..10118
                     /gene="rimP"
                     /locus_tag="IWM51_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP9971367.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10142..11317
                     /gene="nusA"
                     /locus_tag="IWM51_001958"
     CDS             10142..11317
                     /gene="nusA"
                     /locus_tag="IWM51_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP9971368.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11339..11632
                     /locus_tag="IWM51_001959"
     CDS             11339..11632
                     /locus_tag="IWM51_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP9971369.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11629..11940
                     /locus_tag="IWM51_001960"
     CDS             11629..11940
                     /locus_tag="IWM51_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP9971370.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11953..14259
                     /gene="infB"
                     /locus_tag="IWM51_001961"
     CDS             11953..14259
                     /gene="infB"
                     /locus_tag="IWM51_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP9971371.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14286..14633
                     /gene="rbfA"
                     /locus_tag="IWM51_001962"
     CDS             14286..14633
                     /gene="rbfA"
                     /locus_tag="IWM51_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP9971372.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14741..14962)
                     /locus_tag="IWM51_001963"
     CDS             complement(14741..14962)
                     /locus_tag="IWM51_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9971373.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15410..17773
                     /locus_tag="IWM51_001964"
     CDS             15410..17773
                     /locus_tag="IWM51_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAP9971374.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6095 a         3154 c         3920 g         4664 t
ORIGIN      
        1 taaaaaaaga aaaaattatt cagtttttaa ataaaaaaac aatgatttca taggaaaaac
       61 agttagatat tgctataata cttaagatag tgggaatctt ttaagtatag gaggaaaaag
      121 aatgttacgt ttttttccgc aaaaaaacaa atatattcaa gaaaaaagcg aatttcaatt
      181 taataccgat ggaccaattc ctcagcacat tgccatcatc atggacggca atggtcgttg
      241 ggcgcaaaac cgtcgtttgc cacgagttgc tggccataaa gaaggcatgg aaacagtaaa
      301 gaaagtcaca aaaaaagcat caaggcttgg agtgaaagtg ttgacgcttt atgccttttc
      361 gactgaaaac tggaagcgac caaaagatga agtaagtttt ctcatgcaac tacctgtcga
      421 cttctttgat acgttcgtac cagaattgat taaagaaaat gtcaaagttc atgtcatggg
      481 ttatgaaaac gtattgccag aacatacgca agatgctgta agacgtgcaa ttgagcaaac
      541 aaaaaacaat actggaatgg tattgaattt tgctttgaat tatggaagta gagcagagat
      601 cgtgactgct gtccaagaaa ttgccgaaga agtagcaaaa ggagaaatac acgcagaaga
      661 aatcgatgat gaattgattg ctaagcattt aatgacaggt ttcttgccta aagagttaca
      721 agatcctgag ctgatgatcc gtacaagtgg agaagagcga attagtaatt ttctgttatg
      781 gcagatcgca tatagtgagc tgtattttac aaaagcgctt tggcctgatt ttgatggtgc
      841 gcatcttgaa gaagcaatcg cctcttatca aaatcgagac cgtcgattcg gcggtgtaaa
      901 aaaaacagaa gatgagaaag gggatcagtc atgaaacaac gtgtgatcac agcagctgtg
      961 gcgctagtct tgtttattcc gattatatgg atcggaggca tagcggttga gcttacagca
     1021 gcaatcttag cggtagtagg agtttacgag ctgttccgga tgaaaggatt gacactctta
     1081 agttttgaag gagttttatc cgccgttggt gcagtgttac ttgttttgcc aaaagaaaga
     1141 tggtttttct ttcttccaga gaatgtaagt acatttatgt tattttatct tatagttatg
     1201 attcttttag gaacatcagt tatttctaaa aatacgtata cgattgatga agcaggtttt
     1261 cctgtgcttg tcagcttgta tgtgggaata ggtttccaga attttgtcaa tgcacgaaca
     1321 gaaagcttgg ctgttttgat atttggtcta tttattgttt gggcaactga tatcggagca
     1381 tatatggttg gtcgacgtta tggtcagcat aagttaagtc ctgatatttc gcccaacaaa
     1441 acgattgaag gtgcgctcgg tggtatcggt agtgctgttg tagtcgcatt tctttattta
     1501 ttgctatacc cagcaaaaga actattcaat catggtatgt ttgtcatgct tctgatgacg
     1561 attatttttt cagcagtcgg acaatttggt gatttggtcg aatctgcaat caaacgtcat
     1621 tatgatgtga aagactcggg aaatatctta ccaggccatg gtggaatctt agaccgtttc
     1681 gacagtttgc tgtttgtatt tcctatcatg catttatttg gcattattta gtaagtatac
     1741 ctgcgtttct ttgtgaattt cataaaagac gcaggttttt atttttcatt atattgatac
     1801 tattggtgtc tttggtatac tatggtaaaa ttatagtgtt ttatcgttgt tttataaaaa
     1861 acacaaaaaa caagtaaagg agaaaaaagc tatgaaaacg attctgacat ttatcatcgt
     1921 ttttggtata ttagtgattg ttcatgagtt tggtcatttc ttctttgcga aacgatcagg
     1981 aatcctcgtc cgcgaatttg ctatcggtat gggtccgaaa atttatgggc atcaagcaaa
     2041 agacgggaca acatatacgc ttcgcctatt acctattggc ggttatgttc gaatggcagg
     2101 caatggggat gatgaaacag aaatggcacc aggaatgcct ctttctcttt tactaaattc
     2161 tgacggcatc gtagagaaga taaatttaag taagaaaatc caattgacaa atgctatccc
     2221 gatggaattg agccgttatg atttagaaga tgagctgact atcactggtt atgtgaatgg
     2281 agacgaaaca gaagtagtaa cctaccctgt tgatcacgat gcgacgatta ttgaaaacga
     2341 cggaacagaa atcagaattg cgccaaaaga cgtacagttc caatcggcta aattgtggca
     2401 gcgtatgctg accaactttg ctggacccat gaataacttt attttggcaa tcgtgttatt
     2461 catcattctg gcatttatgc aaggtggtgt tcaagtgacg aacactaatc gcgtaggaga
     2521 gatcatgcct aatggagctg cggcagaagc aggcttaaaa gaaaatgatg aggtagtcag
     2581 tgtggacgga aaagaaatcc attcatggaa tgatctgaca acagtcatta cgaaaaatcc
     2641 aggtaaaaca ttagacttta agatcgaacg agaaggtcag gtgcaatctg tcgacgtgac
     2701 accgaaatct gtagagtcaa atggcgaaaa agttggccaa cttggtataa aagccccaat
     2761 gaatacaggt tttatggata agatcattgg agggacgcgt caagctttca gcggttcatt
     2821 ggaaattttc aaagccttag gatcactatt tacaggtttc agtttagata aattaggcgg
     2881 acctgtcatg atgtaccaat tgtcttcaga agcagcaaat caaggtgtaa caactgttat
     2941 tagtttgatg gcgcttcttt caatgaatct cggaatcgtc aatctgcttc cgattcctgc
     3001 cttagatggc gggaaattag tcttgaatat ttttgaaggg attcgcggga aaccattaag
     3061 tcaagaaaaa gaaggaatat tgacattagc tggctttgga tttttaatgt tgttgatggt
     3121 gctagttaca tggaacgata ttcaacgatt ctttttctag aaaaagttga caaatagggt
     3181 ataataacaa acgataacta ttttgaggaa ataaaggagt atatagaatg agacaatcaa
     3241 aaatgttgat tccaacatta cgcgaagttc caaatgatgc agaagtatta agccatcaaa
     3301 tcttgctaag agctggatat atccgccaag tatcagcggg tatttattca tatctgccat
     3361 tagcaaatcg tgtattggaa aaactgaaac gcattatgcg ggaagaattt gaaaaaatcg
     3421 gagcggtaga aatgctgatg cctgccatct tgccagcaga actttgggaa gaatccgggc
     3481 gttacgaaac atatggacca aacttgtatc gtttcaaaga ccgcaatgat cgaaaaatga
     3541 tccttggacc aactcacgaa gaaactttta ctgatttgat gcgtaatgaa atcaattcat
     3601 ataagaaatt acctttaaat ctttatcaaa tccaacctaa atatcgtgac gaaaaacgac
     3661 ctcgcttcgg tttgcttcgc ggaagagagt ttatcatgaa agatgcatat tctttccatg
     3721 caacggaaga aagtttggac gagacatata aagattacga aaaagcctat accgaagtat
     3781 tcaagcgttg tggcttagaa tttcgctcga tcatcggaga cggcggcgca atgggcggga
     3841 aagactcaaa ggaattcatg gcgatctcag aaatcgggga ggatacgatc tgttattcga
     3901 ctgaaagtga ttatgctgca aacttggaga tggctacaag ctactatgta tcaaaaaaat
     3961 cgcatgaaac acagcttgaa ttggagaaag tagctacacc taacgtaaaa tcaattgacg
     4021 aagtagcaga tttcttttct gttgaacctc aaaaaatcat caagtctgta ttgttcattg
     4081 cggatgaaca acctgtcctt gttttggtac gtggagacca tgaagtcaat gacgtaaaac
     4141 tgaaaaactt tttatctgct gactttttag aagaagcaac agaagaagaa gcacaaaaat
     4201 atctaggtgc tgagtttggt tctgtcggac cagtcggtgt ttcagatgac gtaaaagtat
     4261 atgcagaccg ttatgtccaa gatttagcga atgccattac tggagcgaac gaaacaggat
     4321 tccactataa aaatgtgaat ccaaaacgcg atttcactgt gctttcttat gaagatcttc
     4381 gttttgtaca agaaggagat ccttcaccag acaacaacgg tgttcttgcc tttactcgag
     4441 gaatcgaaat cggacatata ttcaaaatcg gcactcgtta cagtgaatct atgggagcaa
     4501 ctgtactaga cgaaaatgga cgagaaaaat ttgtgattat gggctgttat ggtatcggtg
     4561 taagtcgttt attatcagca atcgtagaac aacattcaga tgaacaagga atcaattggc
     4621 caaaaggaat tgcgccattt gatctgcata tcgtccaaat gaatctaaaa gacgaatacc
     4681 aaacaagcct gacgaatgaa ctagaagaag cgatgacaaa agctggctat gaagtactgg
     4741 ttgatgacag aaatgaacgc gcaggagtga aattcgcgga ttctgatttg attggctgtc
     4801 caatcagaat cactgtcggt aaaaaagcag tggatggaat cgtagaagtg aaaatcaaga
     4861 aaactggcga aatggttgaa gtacgtaaag aagaattggc gaatacttta ccaatcctct
     4921 tgaaccaagg cgaataataa gcgttaaaac acagaaaata aaaatagctg aatcagctgt
     4981 ttttgacaat ctgatgattc gcttttaaaa ttggaactgc gatttgaatc gcagttccaa
     5041 ttttttaatg taaaactcag aaaaaacgag tatactaaaa ggtactgaaa aagaagagga
     5101 taagatgcga tcaattgcat tgtttctcat cagaaagggc gtatatcttt gtcagaaaaa
     5161 cgagatttat ttgaaaaact gttggaacaa atccaactag aggaagcaga aaaaaatcat
     5221 ccactgctta ctgctggtga aatggatagt gtagtggtcc atcgtaaatc gcgtttatgg
     5281 gaatttacgc tacatttttc caaaattttg cctattatgc tatatcggtc attgactcag
     5341 catctgacta tcgcttttaa agatattgca caagtcaaac tgaatatctt agcagatgac
     5401 cagacattcg atgaacagct cttgcaagat tactgggccc aagctttgga aaatcagcaa
     5461 tgcgatacac cactagcaca gcaagtattg aaaacgcagg tacctgtgat aaaagatcgt
     5521 aaagtcatct tgccaatcga cagtacagga gctatttctt atttgaagca acaatacttg
     5581 ccattagtag aagagttgtt cgtcagttac ggattcccaa aatttcgtat cgaaccagaa
     5641 gtggatgagc agcaagcgga acgtgtattg aaactttttg aagaacgcaa acaagaacag
     5701 gcagaagcct ttatgaagca agcagctgaa agtctagttg tccacgaaca aaagaaaaag
     5761 gaaagaaaag agcaatcgcc agctcttgaa ggacccatcc agctaggacg aaatattcca
     5821 gccgatgaac caacgacacc aatgatcagt atcgttgaag aagaacgaag agtcacactt
     5881 gaggggtacg tattcgacaa agaagtcaga gaactacgtt caaaacgcaa gatcttgact
     5941 ttgaaaatca cagattatac ttcatcattt atcgtcaaaa aattctcgaa cggcgaaaaa
     6001 gatgaacaag tatttgatgc gatcagtgtc ggtagctggt taaaggtacg aggaagtatt
     6061 caagaagata cgtttgtccg tgatttggtc atgaatgcgc aagatatcat agaagtcaaa
     6121 catacacctc gcaaagatta tgctcctgaa ggggaaaaac gagtagagct tcatgttcat
     6181 agcaatatga gtacgatgga tgcaacaaac agcatctctg atcttgtggc acaagcagga
     6241 aaatggggac accgagctat cgccattaca gaccatggtg gtgcgcaagc ctttccggaa
     6301 gcacatagtg ctggaaaaaa agcaggtgtg aaaatcttat acggcgtgga agcgaatgtc
     6361 gtagatgatg gtgtcccaat tgcttataat gatgcacatg aagcattgag tgaagccaca
     6421 tacgtcgtat ttgacgtgga aacgacagga ttgtctgcag tatatgacac gatcatcgag
     6481 ctagccgcag tcaaaatgta taaaggaaat gtgatcgaaa gctttgatga atttatcgat
     6541 ccaggacatc cattgtcacg aacaacggta gatctgactg gtatcacgga tgaaatggtt
     6601 cgagggtcaa agtcggaaga agaagtattg cggatgttcc ttgaattttc aaaagataca
     6661 attttggtag cccacaacgc ggcttttgat atggggttct tgaatactag ctacgcaagg
     6721 tatggtattc ctgaagcggc caatccagtc attgatacgt tggaattagc tagatatctg
     6781 tatcctcaat tcaaacgttt cggattaggt gtgttatcta aaaagtttgg tgttagcttg
     6841 gaacagcatc accgagcaat ctatgatgct gaagcaactg gtcacttagc atggatcttt
     6901 gtgaaagagg caatggacaa tcataacatg ctttatcatg atcagctaaa tgagcatatc
     6961 ggagaaggag attcttacaa aagggctcga cctttccatg taacgatttt agcaaaaaat
     7021 caggcaggac tgaaagattt gttcaaactg atttcaatgt cgaatgtcga atacttcgaa
     7081 cgcgtcccac gaataccacg ctcccaattg aaaaagatgc gtgaaaatct attgatcggc
     7141 tcagcttgtg ataagggaga aatattcgaa gccatgatgc aaaaaggggt agaagaggca
     7201 agaaaccgag caaaattcta tgattatatt gaagttatgc caaaagcggt ctatgcgcct
     7261 ttgatcgagc aagaattggt aaaaaatgaa catgatttgg aagaaattat ccaaaatttg
     7321 gtagaaatcg gaaaaagttt ggataaaatc gttgttgcaa caggtaatgt acattatctg
     7381 aacgaagaag atgccattta ccgaaaaatt ttgatcaatt ctatgggcgg agcgaatccg
     7441 ttgaatcgtc atagtttgcc ggatgtccat ttccgaacaa ccgatgagat gctaactgca
     7501 tttcactttt taggggaaga aacagcgaaa gagatcgtag tagaaaatac gaataaaatc
     7561 gcagatatct gcgaagaagt gattccagtc aaagatgaac tttatacacc gaagattcca
     7621 ggttcagaag atgaaatatc agaattgagt tacacaaaag caaaacaaat gtacggagat
     7681 ccgttacctg agatcattca aaaacgatta gaaaaagagt tgaattctat taatggtaat
     7741 ggcttctcgg ttatttatct gatcgcccaa aaactagtgc ataaaagtaa tgaagatggc
     7801 tatctagtag gttcacgtgg ctctgttggc tctagctttg ttgcgacgat gacaggaatc
     7861 actgaagtta atccattagc ccctcattat tattgtccag agtgccaata ttccgagttt
     7921 tttgaagatg gtacgtatgg ttctggattt gacatgccag aaaaacaatg tccaaaatgt
     7981 ggtgcacgct taaacaaaga cggtcatgat attccatttg agacattttt aggattccat
     8041 ggagacaaag tgcccgatat tgatttgaac ttctcaggtg attatcaagc agaagcccat
     8101 aactatacga aagtattgtt tggcgaagac tatgtttacc gtgccggaac gattggtaca
     8161 gtcgccgaca aaacagcata tggctatgta aaaggatacg aaagggacaa taatctgcag
     8221 tttcgaagtg cagaagtcga cagattagca aaaggcgcta ctggagtcaa acggacaacc
     8281 ggccagcatc caggggggat catcgttatt cccgattata tggatgtcta tgattttacg
     8341 ccgatccaat atccagcaga cgatcaaaat tcagaatgga aaacgactca ttttgatttc
     8401 cactcgattc acgataatgt attgaaatta gatattctag ggcatgatga cccaactgtt
     8461 attcggatgc ttcaagattt atcagggatc gacccacaaa cgatccctac ggatgacccg
     8521 gaagtgatgc ggatatttgc cggtcctgaa gtacttggag taagtcagga acaaatttat
     8581 tccaaaacag ggacattagg tattccagaa ttcggcactc gttttgttcg agggatgtta
     8641 gaagaaacgc atcctacaac ttttgcagaa ctactgcaaa tttccggact atctcatggt
     8701 acagacgtat ggttaggaaa tgcggaagaa ttgattcgac gaggagatgc gacactggcg
     8761 gaagtaatcg gctgtcgtga tgatattatg gtttatttga tccatgctgg attagatagc
     8821 gggatggcat ttaaaatcat ggaaactgtt cgtaaaggac aatggaacaa gatccctgat
     8881 gaattgcgag aaacctatct atcggcgatg aaagaaaata atgtgcccga ctggtatatc
     8941 gattcttgtt caaaaatcaa atatatgttt ccgaaagccc atgctgcagc ttacgtattg
     9001 atggctcttc gtgtcgctta tttcaaggtt tatttcccta ttttgtatta ctgtgcatac
     9061 ttttctgtac gtgcagacga ctttgatcta gtctccatgt gcaaaggaaa agatgctgtg
     9121 aaacaagcaa tgaaagaaat cacggacaag ggattagatg cttccgtaaa agaaaagaat
     9181 caactgacag tgctggagtt agccaatgaa atgctggaac gaggattcaa atttggaatg
     9241 attgatttgt ataaatcgga tgccgttaac tttgttattg aaggtgatac gttgatcgca
     9301 ccatttagag ctgtcccaag tttgggaacc aacgtggcga aacaaatcgt tgaagcaaga
     9361 aaagacggtc catttttatc caaagaagat cttgccaccc gcggtaaagt ttcaaaaaca
     9421 ttgatcgaat atatgaacga caatggcgta ttgaaagatc taccagatga aaatcaatta
     9481 tcgttattcg atatgctata agcatctgtt ttcctgtctg cttgtaattt taagcagagt
     9541 atggtatagt tcttataggt aatgatacta actgtgagtg agcggatttt tcgctcactc
     9601 tttttaatgg aattacgcac ttaattttta aggaggcgaa acttttgagt agcgtcgttg
     9661 aaacagtcac agaaatggtg acaccgattt tagatgaaca aaattttgaa cttgtcgaag
     9721 tcgaatttgt aaaagaagga aaaagctggt tcttacgagt atttattgac aaagaaggcg
     9781 gtatcgatat cgaagaatgc gcctttgtca gtgagaaact gagtgaaaaa cttgatgcag
     9841 cagatccaga tccgattcct caagcttatt tcctggaggt atcctccccg ggagcagagc
     9901 ggccattgaa aaaagaaagc gactatgaaa aggcacttgg tgattatgtc catgtctcct
     9961 tataccaacc agtagatggc gaaaaacaat atgaaggatt tcttcaatca tttgatgctg
    10021 aacagcttac attgaaaata cgcataaaaa cacgagaaaa agaattgact tttgaccgaa
    10081 aaaatattgc aaaagctcgc ttagctatcc aattttaatc acggagagga atcagaaaaa
    10141 aatgagtaaa gaaatgttaa atgcgttaga cgctttagaa gctgaaaaag ggatctcaaa
    10201 agaaatcgtg atcgatgctt tagaagcagc attggtttct gcatacaaac gtcactatgg
    10261 ccaagcacaa aacgtcgaag tggaatttga tcagaaaaaa ggaaagatcc atgtctatgc
    10321 ggtaaaagaa gtaacggaag aagtgatgga ttcacagttg gaagtctcat tgaaagatgc
    10381 acttttaatc aatccagcat atgagatcgg tgatacgatc cgttttgaag taacaccaaa
    10441 agattttgga cggatcgctg cgcaaacggc taaacaagtt attttacagc gtgtacgtga
    10501 agcagaaaga acgattattt ataacgaatt cagtgcttat gaaaaagaca tcatgcaagg
    10561 aatcgtcgaa cgccaggata aacgttatat ttatgtcaat cttggtaaaa ttgaggcagt
    10621 tctttctaaa caagatcaaa tgccaaatga attttatcaa ccacatgatc gaatcaaagt
    10681 ttatgtttcc cgagtagaaa atacgtctaa aggtccgcaa gtatttgtta gccgcagcca
    10741 tccagaccta ttgcgtcgct tatttgaaca agaagtacca gaagtctatg atggaatcgt
    10801 tgaaattgtc agcatcgccc gtgaagctgg cgatcgttca aaagtggcag ttcgctcaaa
    10861 tgatccaaat atcgatgcag tcggtacatg tgtcgggcca aagggacaac gagttcaagc
    10921 aatcgtcaat gaattaaaag gcgaaaacat ggatatcgtc gaatggaatg aagatccagc
    10981 tgtattcatc gcaaatgcat tgaatcctgc acaagtcatg gatgtgatct tcgatgagac
    11041 aaatccaaag gcctgcacag ttgtcgttcc agactaccaa ttatcgctag caatcggtaa
    11101 acgcggacaa aatgcacgtt tagctgctaa attgactaac cataaaatcg atatcaaatc
    11161 agaatctgac atgacggaat tttatgaaaa tcaagaacaa caaaaagaaa cagaagaact
    11221 gcatgatgaa gcaatcgttc agtcagattt aacagacgat gaatatgaaa caattgcatt
    11281 caacaatgaa acggtcgaag aaaaaccaga agcttaactt tagtaaggag gcaaagagat
    11341 gaaacaaagg aaaattcctt tacgcaaatc cgtggtttca ggtgaaatga aacctaaaaa
    11401 agagatggtc cgaatcaccc gttcaaaaga aggggaagta tcgatcgatc caactggaaa
    11461 aatgcctgga cgaggagcgt atgtctcact tgagccggaa gaagtacaac aagcttggga
    11521 taaaaaaatt ctcgatcgag tactcgaaac aacattaaca gatgagtttt atcaagaact
    11581 tcttgactat gtcactcacc aaaaagcacg taaagagtta tttggaaaat gaatacagtt
    11641 aatcggcaaa aagccatgaa tctcatcggt ctagcgatgc gcgcgggaaa actaatcacg
    11701 ggtgaagaac tgaccattgc tgaaatccgc aaacaaaaag caaaattggt gtttgtggca
    11761 accgatgcaa gtgagaatac aagaaaaaag ataaaagata agagttcata ttatgaagtt
    11821 ccttgctttg agctattttc tgaagcagaa atcacgcaga tgatcggaaa acctcgcaag
    11881 gtattcggaa taatggatgc cggatttgcg aagaagacca aggagctaat tgaaggttag
    11941 gaaggtgatt gcatgggcaa caaaagaatc tatgaattag caaaagaact caaaaaatct
    12001 agtaaagacg tcgtggaaaa agcacaacaa cttggcatcg atgtgaaaaa ccatatgggt
    12061 gcgattacta gcagtgatga gaagaaattg caacaagcgt tcaaaaaccc aagcacgaaa
    12121 caacaagctg cacaacaagc aagcagaaaa ccagccaatc aacagccgaa cgatcaacgt
    12181 aaaaaagaaa caacgaacaa tcaacaaaaa aataaaaata accgcagtta tcaagatcgc
    12241 ggtcaaggga gcggtcaagt gaatcaagga aaaaaaccaa caacaaacca aaataatacg
    12301 ggaaatacca gcagccaaaa tcgtcaagga acgagccaag gcggcaatca aaaccgtact
    12361 aacaataaca acaaaccaaa caataacaat cgcggcaaat ttaataataa taaccgtaat
    12421 cgttataata aaaaaggtaa aaaaggcaaa cagcaacaat caactaaacc agctgtacca
    12481 ccacgcaaat tccgtgaatt gccagatgta ttagagtata ctgaaggtat gaacgtagca
    12541 gatattgcga aaaaaattca tcgtgagcca gcagaaatca ttaaaaaatt atttatgctt
    12601 ggtgtcatgg tcaatcaaaa ccaagcactg gataaagata cgattgaact gttagcgacg
    12661 gactatggca tggaaccaca agaaaaagtc caagttgaca ttgctgatat cgataaattc
    12721 ttcgaggcag aagaagtcga tccagataaa ttagtctcac gtccgccagt agtaacgatt
    12781 atgggacacg tagaccacgg gaaaacaacc ttattggata cattgcgcca ttcacgtgta
    12841 acaagcggcg aagccggcgg aatcacacag catatcggtg cctaccagat cgatattgat
    12901 ggtaaaccaa tcactttctt ggatacacca ggacatgcgg cctttacaag tatgcgtgca
    12961 cgtggagcaa gtatcacaga tatcacgatc ttagttgttg ctgcagatga tggcgtcatg
    13021 ccacaaacag ttgaagcaat caaccatgcc aaagctgcag gcgttccaat catcgtcgct
    13081 gtaaataaaa ttgataaacc aggtgcaaat cctcagcatg ttatgcaaga attaagtgag
    13141 tatgaattaa ttcctgaagc atggggtgga gaaacgattt ttgttgagat ctcagcgaaa
    13201 ttcggccaaa acatcgaaga attgctggaa atgatcctgt tagtcgcaga agtagaagat
    13261 ttaaaagcag atccaacaca acgcgcaatc ggaacagtga tcgaagcacg tcttgataaa
    13321 ggtaaaggtc ctgtgtcaac cttacttgtc caagaaggaa cacttcacgt tggtgaccca
    13381 atcgttgtcg gtaatactta cggacgtgtc cgtgtaatga caaacgatct tggccgtcgc
    13441 gataaagaag caggacctgc aacgccagta gaaatcactg gcttgaatga tgtacctcaa
    13501 gcaggtgacc gtttcgtagt cttcgaagac gaaaagacag cacgtgcagc gggtgaagaa
    13561 cgcgcaaaac gtgcattatt ggaaaaccgt gctgccacaa gccgtgtgac tttggacaac
    13621 ttatttgaaa gcctgaaaga aggcgaattg aaagaagtta acgtaatcat caaagcagat
    13681 gtacaaggtt cagcagaagc actggctgca tcattgaaga agattgatgt ggaaggtgtc
    13741 cgtgtaaaaa tcgtccattc agctgtcgga gcaatcaatg aaagtgatgt aaccttagca
    13801 gcagccagca acgctattat catcggtttc aacgttcgtc ctacaccgca ggcaaaagtg
    13861 caagctgata ctgaagaagt cgatattcgt cttcaccgta tcatctataa agccatcgaa
    13921 gaaatcgaaa ctgcgatgaa agggatgcta gaccctgaat ttgaagaaaa aatcaccggt
    13981 caaatgatcg ttcgtgaaac cttcaaagtt tctaaagttg gtacgattgc aggtgcctat
    14041 gtaacagaag gatatattcg tagagatagc ggtgtccgtg taatccgtga cggaatcgta
    14101 atctatgaag gccaacttgc aagtctgaaa cgattcaaag atgacgtgaa agaagtcaaa
    14161 atgggttatg aatgtggtgc gatgattgaa aaattcaatg acatcaaagt agatgacgta
    14221 attgaaggat ttatcatgga agaagtcaaa aatgactaaa aactgataaa aggaggccaa
    14281 tcattatggc taactaccgt gatcgccgag tcggtcaaga aatcttaaaa gaagtcaatg
    14341 atattttgcg caaaaaggtc cgtgaccctc gtgtggagaa cgtaacgatc acagatgtga
    14401 aagttacggg ggacttacag caagcaacga tctattacag cattttatct gacttagcat
    14461 cagataagaa aaaagcacaa gatggattga ataaagcatc aggattgatt cgtcgagagt
    14521 tagggcacaa tctaagtatt tataagacac ctgaattgtt ctttgaactt gatgaatcag
    14581 ttgtctatgg gaaccatatc gatgaattgc ttcgcgactt gaacaaagac taatcaaaaa
    14641 atcccgcaaa tcctgtgaaa tcaggggttg cgggattttt ttgttttcat taagacgtat
    14701 tacctaagag ttccatgact ttagtctggt agcgtgtcag ctaaccaaaa aagagaaaaa
    14761 gaaaagtaaa aaattcgact ttcttttttc tatagtatat aaatttttgc atatagataa
    14821 agatcaagat accgataacg gaagctagat tgtaatctat tttagtgaca gtcaggtata
    14881 gccaagtaac tagaagatca gtggaacctg caccataaaa gtttgagttt gtcggtccgt
    14941 tgacagttaa aagaaaaatc acgttgaagt tattgatatt tccgattacg ccgcttcgat
    15001 ttggtcacga tccaaatttt ggataatccc tgtagaaatt agcatagtag ctgggatacc
    15061 aattcacata ttgacaaaga taacagagac ttttacataa aacggatcaa tcaggaaagg
    15121 aattgctcga tccagcccca gttttccaac aatgtgttaa ctggcctagc gtcattcagc
    15181 aaattatgca tgatgaggag cgaaatgaac tgcggaacag ccattgtgat aacgacaagt
    15241 tttttggatc aatgtaaaaa acgtcagcga aaggaaaaat ttcgtggttt tttatacctg
    15301 ttcatttggc tagatataaa atccaaacaa atgaacagaa gtgcttatta ggtttcaatt
    15361 gggagtatct gcgctagaat aagagtaata atgaaaagga gtgttatgta tggattattc
    15421 tagtaaagaa tactttgata aaatgacggc ttggtggcgt gctgctaatt atctttctgt
    15481 tgggcaactt tatcttaaag ataacccctt actgagacgt acactgaaac cagaagatgt
    15541 caaaaaacat ccaatcggtc attggggaac gattcctggc caaaatttta tttatgtcca
    15601 tttgaaccga gtcatcaata aatatgattt gaacatgttt tatatcgaag ggccaggaca
    15661 cggcggacaa gtcatggtat cgaatgctta tttagatgga agttacacag agatctatcc
    15721 agaagtaacg gaagatgaga caggaatgca aaaattattc aagcgatttt catttcctgg
    15781 aggcattgct tctcatgcag caccagaaac accaggttct atccatgaag gcggagagct
    15841 tggatactct ctttcacatg cagtcggagc tgtattagac aatcctgaag taatctctgc
    15901 tgtcgtgatt ggtgatggtg aagcagaaac cggtccttta gctggaagct ggttctcaaa
    15961 tgtctttatc aatccagtga tagatggagc tgttttaccg atccttcatt taaatggtgc
    16021 aaaaatagcc aacccaacga tcttggcacg taaatcagat ggagaattag caaattactt
    16081 taacggactt ggctgggagc ctttctttat tgaaggaaat gatccagaaa aattaaatcc
    16141 tgttatggct gaaaaaatgg atcaagcaat cgagaaaatc aaatccatcc aaaaagaagc
    16201 tcgcctgaag acagcaacag atgtcgtgat gccaaaatgg ccagtattga tcgtacgcac
    16261 accaaaagga tggaccggtc caaaagaatg ggacggggaa ccaattgaag gaaccttccg
    16321 agcacaccaa gtaccgattc ctgtagatca agaacatatg gatcatgcag atgctttact
    16381 ccgttggctg aaatcctatg aaccagaaaa attatttgat gcacaaggac gtattttaga
    16441 agaaatcaga gaaattgcgc ctactggtga ccaaagaatg gcaaaaaatc ctatcaccaa
    16501 tggtggaatc gatccaagac cgcttattat gccagattgg aaaaaatata ctttgcaatt
    16561 tgaaaaacca ggctccgtta aagcagaaga tatgaccgaa cttggtaaat tcgttcgcga
    16621 gatcattgag aaaaatccag aaaatttccg tatatttgga ccagatgaaa caaaatcaaa
    16681 ccgattgaat caagttttca aaacgacaaa tcgccaatgg atggaaaaaa tcgaaccaga
    16741 aaatgatgaa tggctttcgc catctggtcg cgtcatcgac agtcaattat cggaacacca
    16801 agatgaaggg ttcttagaag ggtacgttct gactggacgg catggttttt ttgcaagtta
    16861 cgaatcattc ttgcgagtgg ttgactctat gttgactcag cacttcaaat ggatacggaa
    16921 atcacatgac ctttcttggc gaaaagatta tccttcgctg aatctcattg caagctcaac
    16981 agtcttccag caagatcaca atggatatag ccaccaagat ccaggaatat tgacacattt
    17041 ggcagaaaag aaggcggagt tcatccgtga atatctgcct gcggatgcaa atacattgct
    17101 cgcagtcatg gataaagcat tccgctcgtc agaaaaaatc aatttgatca tctcttccaa
    17161 acatccgcgt gctcaatttt actcagcaga agaagcagcc gtcttagtga atgaaggctt
    17221 gaaaatcatt gattgggctt cgacagcaaa agaggaggaa cctgaattgg tcattgctgc
    17281 tgcaggaacg gaatctaact tagaagcatt agcagctgta actttgctgc tggaagaatt
    17341 cccaaaacta aaaattcggt tcatcaatgt cgtagattta ttgaaattac gtcatccaag
    17401 tcaagatccg cgtgggttga gcgatgaaga atttgatcaa tattttacaa aagacaaacc
    17461 gatcttattt gctttccacg gttatgaaac attggtccgc acgattttct ttgatcgtca
    17521 taaccatcat ttaatgattc atggctacaa agaaaatgga gatatcacga caccatttga
    17581 catgcgtgta gtaaatgaac ttgatcgtta tcatttggca aaagacgcag ctttaaaaat
    17641 caaaggaagt caagcagaag attttgcgga aaaaatggat cagaaattac aagaacatca
    17701 aaactatatt cgtgaaaatg gtattgatct gccagaagta ttggattgga aatggaaaaa
    17761 tcttgaccaa taaaatgata aaatcgatcc cgcaaaacag aattttttac attgtattat
    17821 ttgtcaaatt aag
//
LOCUS       DACDYJ010000056        17351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDYJ010000056 DACDYJ010000000
VERSION     DACDYJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.056"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(89..1108)
                     /locus_tag="IWM51_001965"
     CDS             complement(89..1108)
                     /locus_tag="IWM51_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9971375.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1130..2137)
                     /locus_tag="IWM51_001966"
     CDS             complement(1130..2137)
                     /locus_tag="IWM51_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9971376.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2130..2795)
                     /gene="pgmB"
                     /locus_tag="IWM51_001967"
     CDS             complement(2130..2795)
                     /gene="pgmB"
                     /locus_tag="IWM51_001967"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9971377.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            complement(2788..5082)
                     /locus_tag="IWM51_001968"
     CDS             complement(2788..5082)
                     /locus_tag="IWM51_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9971378.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5342..7507
                     /locus_tag="IWM51_001969"
     CDS             5342..7507
                     /locus_tag="IWM51_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9971379.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7811..8644
                     /locus_tag="IWM51_001970"
     CDS             7811..8644
                     /locus_tag="IWM51_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP9971380.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVTNEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8746..10446)
                     /locus_tag="IWM51_001971"
     CDS             complement(8746..10446)
                     /locus_tag="IWM51_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971381.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10953..12113
                     /locus_tag="IWM51_001972"
     CDS             10953..12113
                     /locus_tag="IWM51_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9971382.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12129..12572
                     /locus_tag="IWM51_001973"
     CDS             12129..12572
                     /locus_tag="IWM51_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9971383.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13055..13585)
                     /locus_tag="IWM51_001974"
     CDS             complement(13055..13585)
                     /locus_tag="IWM51_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP9971384.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13690..14241
                     /locus_tag="IWM51_001975"
     CDS             13690..14241
                     /locus_tag="IWM51_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9971385.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14394..14867)
                     /locus_tag="IWM51_001976"
     CDS             complement(14394..14867)
                     /locus_tag="IWM51_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9971386.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15005..16477
                     /locus_tag="IWM51_001977"
     CDS             15005..16477
                     /locus_tag="IWM51_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9971387.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16474..17184
                     /locus_tag="IWM51_001978"
     CDS             16474..17184
                     /locus_tag="IWM51_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP9971388.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         5398 a         3118 c         3570 g         5265 t
ORIGIN      
        1 gataaacaat gtgttagaag tagtatcttc ttttacataa aaagctgacc ataggataaa
       61 tggtcagcgt atttagtgag tcagctattt agtatgggtt ttctaaagga acgactgtct
      121 ctcttttgat caagcgatga gggatgacca aacgtacgcc actgctcaat tcttcatgga
      181 tcaattcgcg cagcttttgc atagcgacac gtcctaattc ggagatatcg atatcgatac
      241 ttgtaaggta agggtgtgtt aatgttgaga agatcgagtt gttgaagcta atgacggaga
      301 gtgtctctgg aatgacatag ccgtacattt gcgccaactg catcgtgcga agggcgaaga
      361 tatcgtcaat gaccaccaat gctgtggctt cgttttcttt caacgttttc tcaaaggaca
      421 gataatcctc cggatgctct agtactacac tggataacgc aggaagacca gccatcatca
      481 ttgctttttg ataaccgaaa tatcgttcga aatacaaact ttcatgagtc gtgtttgtca
      541 caaacaggat attcttatgt ccattctgga tcaaaaattc tgttgcttgt ttacctagaa
      601 gctggttatc attatctaca taaacgattt cgttctcatg atgataaggc tgtccgatca
      661 aagtgaaagg gatgtggttt tggtataaat aatcaatgac aggatcttga ctatctgaat
      721 acactagaat aaaaccatct acttgttttt gtaaatgcat acgctggacg ttctctaaca
      781 gcaattcaaa atttttagca gtagcgattg ctgtagtcat atcataattc cgcgcttctt
      841 cgttgattgc ttccatgatc tcaagataaa atgggttacc gatccgctct tttgaatcaa
      901 gagggggtaa gattacgccg atggcactgg aaatgcgttt tcctagattg cgggcagtca
      961 tgttaggaac ataacctagt tcatccataa tttttcggac tttcttcttt gttgtctcgg
     1021 aaatactagg atgatcgttg atgacacgag agacggtcga ggtggcaaca ccggcttttt
     1081 tcgccacatc ttttacggta atagccatga taaaaacacc tttctcttgt tattgtgcag
     1141 agaaccgata aatcgtctga ctgatccgtt cttgaccagg atggaggttg atcgatccaa
     1201 atttggggaa atgaacgata tcagggattt cttgcggttc gattgctaat ccgtagtttg
     1261 aatgcatctg ttttccattg acagagaaat cagcttcgaa tccagtcgta gaaaataata
     1321 ccatactttt attagaagtt gctatagtca tttggcgacc agaatgcttc tcgaataatt
     1381 gtaaggaagt tttggaaagc ctaggatgat gcaaaagaaa tacatcatct agtccatctg
     1441 gataacatgc aaggatctct tgcagtgttg gggattttct aaagtcgatt ggcagttttt
     1501 ctttttttgt ccaatttcca gtaggcagtt tttcttgatc taattcaagc acaccatcta
     1561 aaaagaccgt taactgatgt tcatagatgt ctcttgttcc atttcctgaa aggttgaagt
     1621 aggcatggtt ggtcggattg cagatggttt caacaaggct actagcagag gttgtcatct
     1681 ctaattcgtt ttttgttaaa cggtaagtga tcgtagcagt gattggtcct ggataggtag
     1741 aaaattcatc ttttaaataa aagaccacac ctattgatga tggagttttg aaaacttcta
     1801 cttcccaaaa ttggaaagac cagcctttcg tgccaccgtg gatatggtgt gatccgcagt
     1861 ttttctccaa tctatgctca ccccaagaag catcccggat ccttccggct acaggtccga
     1921 cagttgctcc aaaaaagctt ttgtctgtta gaacatcttc gaaacgatcg taagacaata
     1981 aaacattttc tgattttcct tctttgtctg gtgcgaagat ttggtgcagg cgggcaccgt
     2041 aattgagaag aacgacttct aagtagtcgt tcttcaatgt gattttcttt atttcagtct
     2101 ctggaactgg tacgacggag atctctggat tatccatggt catgacactt ttctttcaag
     2161 taatcgatcg taagaaaagt cgtatcaggg acaagcggga tctcctctcc aaggtcttca
     2221 gcacgtccca cgccaattgg ttgagcgcca cttgagagga tggcttggat accggcttgc
     2281 gcgtcttcga tcccgataca ttcttctgct tttagtccga cagcttttgc tgcaaggagg
     2341 aagatatctg gcgcaggttt tccgtttgtt gcttctgccg ggtcggcaat agcatcaaag
     2401 taaggtgtaa gacccatttt ttcaagtagg aatggcccgt ttttacttgc agaagcaaga
     2461 gcaatcttga ttttttcttc ttttaaagaa tccagtaaag gcacgacccc ggggaagaca
     2521 tcttttggtt cgatcgtctg gatcatctct aaataataat cgttttttct tttcgctaat
     2581 tctgcaaatt cttcctttga aaaagagtgt tcttttccgc cataagctaa aagcaaagag
     2641 agtgagtctt ctcggctgac gccttttagc tgttcgttga atgtgtggtc gatcgtaatg
     2701 ccgatttcct gaccaagctt tcgccaagca cgataatgga attcagcagt atcggtaatg
     2761 acaccgtcta aatcaaacaa taccccttta aacatttgct tcctcctttc ttagcgttat
     2821 ttctagacga tccgctaaag aataagactg ttcatagacg taaatttcta gaggaaaacc
     2881 ttctaataac tcgaaagtaa cttgttcatc tgtgactgag atgaacaata aacgtccgcg
     2941 ataattgata tggaaagaat aagctttcca agtttttggc agaaatggtg cgaatctcaa
     3001 ctgatcatgg gcagttttca tttgagcaaa tccttggaca atcgacaacc agctaccagt
     3061 catggaagtg atgtgcaagc cgtcttctgt gtcgttattg taattatcca aatccaagcg
     3121 agctgttcgc tggtacattt ctacagcttt gctttccatt cctaattctg ctgctaaaat
     3181 cgaatggata cttggcgaaa gggaagactc atggacagtc attggttcat agaaatcaaa
     3241 gtttttttgc ttttcttctt ttgtaaaagt atcattgaag aaataaatcc cctgtagtac
     3301 atctgcctgc ttgatgaagc aagatctaag gattttgtcc caagaccaat gctgattcag
     3361 aggaagatcg cttgtgggaa tatcggatac aggcatgaga tctttatcta agaatgtgtc
     3421 atgctggaca aagattccta attcttcgtc aactgggaag tacatgttct ctgcgatatc
     3481 tttccatttg gctagttctt cttcgctgat agtgatagca gcttcttttt gataagttaa
     3541 ataattttct cgcgtgtaac gcaagaccca agctgcgatc gtatttgtat accaattgtt
     3601 attgatattg ttctcatatt cgtttgggcc ggtcacaccg tggatcatgt atttatttgc
     3661 tcgttttgaa aaatggacac ggtctgccca gaagcgtgcg atttctgcta aaacttccag
     3721 tccttcattt ttcaaatatt ccgtatcgcc ggtgtaattc gtataattgt agattgcata
     3781 ggcaatcgcg ccatttcggt gaatctcctc gaatgtgatt tcccactcgt tatggcattc
     3841 gacaccagtg aaggtgacca taggataaag cgcgcctttt aatccttgtt gctgagcgtt
     3901 gtggattgct tgcggcagtt gattatgccg atatttcagc agattcttag ttacttcagg
     3961 tttggctaaa gcaagataaa gagggacagc atacgcttct gtatcccagt aagtggctcc
     4021 gccgtatttt tctcctgtga atcctttggg gccgatattc aatcgttcat cttcgccgta
     4081 gtaagtagag aatagttgga acaaattgaa tcgaatacct tgttgtgctt cttcgtctcc
     4141 ttcgatgacc acatcggcaa gcttccatcg ttctgcccaa gccgctgttt gctcttcttt
     4201 tgcttggata taattgtttg tctggttcgt taattctgag acagcttttt ctgcttgctg
     4261 cgcttctgga atatctctac tagtaagaac gataatttct ttttccactg accatgtctc
     4321 accttgagat aattctgcag acaagcaacc atgtaatgct aaagtatctt cgtgatagtc
     4381 agggatgact gattttccat taacgaaatg gcgcattgct gcagtaacag taaattgttc
     4441 aatcccaaag ttgtttggaa tagttttagt cgtcaaatag ccgatttctt ctatagttcc
     4501 acgatcaatc tcattccaaa aatgttcatc atagttgctg tcttcgtttt gaacatttcc
     4561 atccaatttt gatacgagtt tgactgttcc gctaccttca agcatttcta tagttaaata
     4621 gatataagct gattcttttt tgacaatgct cagaaaacgt tcgaaggaaa tgcgtacttt
     4681 attagtagaa gtagtgattg tgaatgtacg agataataca ccatttttca tatctaattc
     4741 ccagtaaaag tcttctggtt ccaaagaagc caagtcgatc agctgatcgt tgatcgaaat
     4801 atctatcgcg ataaagttca aggcgttgat cacttttcca aagtattctg gataaccatt
     4861 tttccaccag ccgacacgtg tcttatcggg ataccagaca cccgccaaat aagtaccttg
     4921 atggtggtcc cctgagtaag tttcttcaaa gttcccgcgc atccccatat aaccgtttcc
     4981 aatacttgtt aaagattctt gcagtcgaag atttttttta tctaattgtg tcgtgctgat
     5041 tttccatgga tcaatagcaa atagtcgttt gatctgtttc atttttctcc tcctaatgag
     5101 ctgttttata cgttcataat aaacgcaacc gattgcacaa gtcaatagac aaaagaaaat
     5161 atttcataga aaaaagctta aaaaaataaa tttttaggag aaaattatag aagggaacac
     5221 acgtattata ggttttatag aatggtatag acagaaaaat aaaaaataaa gcatttacaa
     5281 aaaagaaaac gcacgctata atacgtttaa cgaaaccgat tgcgtaaagg agctaaacgg
     5341 aatgaaaaag atatttagtt ttgagttttg gcaaaaattt ggaaaggctt taatggttgt
     5401 ggtagcagtt atgccggcag ctgggttgat gatcagtata gggaaatcaa ttcccttgat
     5461 caatcctgat tggacgccgc ttgtcacaac gggcggagta attgaaaata ttggttgggc
     5521 aattattggt aatcttcata ttttatttgc tttagcaata ggaggaagtt gggcaaaaga
     5581 aagagcaggc ggagcatttg cagctgggat ctcatttatt ttaatcaatc ggatcactgg
     5641 ggctatcttt ggtgtcacaa gtgacatgct tgcgaatgaa gatgcattta cccacactct
     5701 gttcggtacg aagatcatgg ttaaaggatt ttttacaagt gtattggaag caccagcttt
     5761 gaatatggga gtattcgtag ggatcatcgc tggatttgtc ggggcaatgg cttataataa
     5821 gtactataat tatagaaaat tgccggatgc tttgtcattt ttcaatggaa agcgctttgt
     5881 tccgtttgtt gttatcttat ggtcaacaat cgtggcattg attcttgctg tggtgtggcc
     5941 aaacgtacag gctggaatca ataattttgg tttatggatt gcagaatcac aagaaagcgc
     6001 accgattcta gcaccgtttt tatttggaac attagaacgt ttactattgc catttggatt
     6061 gcatcatatg ctgacgattc ctatcaacta tacacagctt ggcggatcgt atgagatttt
     6121 atcaggcgca caggcgggga cacaagtatt tgggcaagat ccgttatggt tggcttgggc
     6181 cactgattta gtgaatctta aaggtgcagg ggatatgtcg caatatgagt ttgtgttgca
     6241 taactggacg ccggctcgtt tcaaagtagg acaaatgatt ggttcttcag gtatcttgat
     6301 gggaatggct tttgcgatgt atcgtaatgt cgatcctgat aagaaagcaa gatataaatc
     6361 aatgtatttt tcagcagcgt tggctgtctt tctaacaggt gtaacagaac cattggagtt
     6421 catgtttatg tttgctgctg tgccattata tgtcatttat tcagtgatcc aaggggctgc
     6481 atttgcgatg gcagatatct tgccattgcg ggtccattca tttgggaata tcgaattgct
     6541 gactcggaca ccgttagcta tcaaagcggg tctaggtgga gatcttatca acttcgtgtt
     6601 gtgtgtgatc gcttttggtg ttgttacgta tttcttagca aatttcttga tcaaaaaatt
     6661 caactttgct acaccgggac gtaacggaaa ttacgacaat gacagtgaag aaaccgttgt
     6721 atctaacagc ggaacaggaa cagcagatca gcaagtggtc cagatcattc atttgctggg
     6781 aggaaaagaa aatatcaaag atgtcgatgc ttgtatgact cggttacgcg taagtgtaaa
     6841 tgatcgagaa aaagtaggga ctgaagaagc ctggaaacga gcaggggcga tgggattgat
     6901 cgtcaaagac aatggagtcc aagcagttta tggaccaaaa gcagatgtct tgaaatcaga
     6961 tatcgaagat ctgctgcaat caggagcaga gattccagag ccagaaatgg tttctacagg
     7021 aaatggacaa gcaggtagta agcaatactt aggtatagaa caagaactag tttccgcagc
     7081 ttctggagaa gtgattcctt tatctgaagt aaaagatccg gtattctcac aaaaaatgat
     7141 gggtgacggc tttgctgtta ttccaacatc aagagaagtt gttgcgccga tcgctggaaa
     7201 agtgacaagt attttcccta gcaaacatgc aattgggctg gaaacgaaag atggtatcga
     7261 agttttgatc catatgggaa tcgatacagt ccaaatgaat cagccagctt ttgagattgc
     7321 tgtcaaagaa ggccaagaag taggaacagg aacgaaatta gctgagatgg atcttgatgt
     7381 gatccaaaac gaagaaaaag atccaacgat catgatcgta tttacaaacg ataaagtaga
     7441 agaagtagtg atcaagcaat taggtacagt gacagctggt aaagtgatcg gctccattaa
     7501 aatgtaagga gaggttgtga aaaagctgtc ctgcatcaag tcataagaac agccaaacaa
     7561 aaatagctcc tcctattttt cgtttggttg ggcttattac cgagaagcag cagtttgaat
     7621 actgtttagt ggagaggtcg tgaaaaagct gccctgcacc aagtgataaa aacagccaaa
     7681 caaaaaatag ctcctggtcc attgagctta ttactgagga gttagcagga gaacactatt
     7741 tgctggagag gttgtgacaa aaattttgtc acaacccttt tgtttcgaaa tgaagacaag
     7801 gagaaaacca atgaaattca tgacactcaa cacgcatagc tggcttgagc cagaaccaga
     7861 gaaaaaattg caagaattag cagataagat cctgttagaa gattatgaag tgattgcttt
     7921 acaagaaatc aaccaattac tggaatctga agaagtagaa cctgcgaagc taatgaagtt
     7981 ctgtcaggta aagaatcaag tacctatccg taaagataat tttgcctacc gtttagttca
     8041 gcttttaaag gaacacggaa aagagtattt ttggagttgg gaaatgagcc acatcggtta
     8101 tgataaatat gaagaaggaa atgcactgct tacgaaaaaa acattggaaa gtcaggtcct
     8161 taccgtctcc caatctcaaa agaaagaaga ttatcagaca cgcaagatct tgattgggaa
     8221 aacaaagata gatgatcagg acatttttgt ctcgagctgc cacttttctt ggtggacaga
     8281 taaaaaaagt ggcttttatt ttgagtggaa aaacttggaa aactactttt tggaaactcg
     8341 cgtaccactt tttttcctag gtgatttcaa taatccagta gactctcaag gttattatac
     8401 tgtaagagaa agttgccttc ttcttcaaga ttcgtatgtt gtcacgaatg aaaaaggaaa
     8461 agcagctact gtggaaaaga aaatagatgg ttgggaacaa aatacagaaa aattgcgtat
     8521 agattttatt ttcgtccctg aaggaatgca ggttaaaaaa tatcaacgga tttttgatgg
     8581 gatcgatagt ccaatcatca gtgatcacta cggggtggaa atcgaattag atgtgaacga
     8641 ataaaaaata taacatataa cataaaaaaa ctgattaaaa taaaactctc caaacgatta
     8701 taccgtttgg agagtttttt gatcggatgt atttatccct tttgcctaag cacgtaaaag
     8761 cttgtatcta tgataaataa ataaacatag caaggtaaac cagccaatca aagtaagaga
     8821 aagaatgaaa tagaaataga ttggcgtttg ataatgaagc gtgagctcgt tgattccttt
     8881 tttctgcttc acgattgggg tcccgatatc tgttacttca taatcagtca atgtttttcc
     8941 attttgctgt agcacagtcc gatcgtagac gataacaggg atatgaacag ttttgtcccc
     9001 agtgtttttc cacttgattg tcaaaagacc gcctggatgt gtttttataa aagttgtttc
     9061 gttttctagt atttcttcgt tataccggtc gtatttattg tcttttgtct ccttatagat
     9121 tggcagatag tcaggtgtgg ttttttggaa aacgaacaag ctggtagaca tatctttgct
     9181 gaagaatgtt ttccttgctt cttccggcgt atcgaataga taagtatgtc gactgacaaa
     9241 tgtttcattt tcccactttt ccagatgctg gtataagttc tggatcgtct gtgctgaaaa
     9301 aataagaatg ccaaatatac ttaaaagacg gaaccacttt tgttcagacc actggttcag
     9361 tgaaagggca aggtaaagta gaagcaatag cgtaaatggc acgaaaaatc gaaatggaaa
     9421 ctggatcaaa ctaataaaag gcacattttt ttcgatcaat gtcgtccatg gaatcaagct
     9481 agtagaaaga aaaaagaaaa caagacttgt gtacaacacg attttttgga ataaggtgtt
     9541 cttttgttga aagaaaataa aaagcaaaaa acctgataat atcaatatag gaaaggcctt
     9601 agggtaatgg agccaataac tgccatccag atctatcgcc ttttgactca tttctctgtt
     9661 aacaaaggga ggaagaatct tattcccact gtaaacaata gctaatcctg cccaaatatt
     9721 tgctgtcaga acaaaataaa gcaagaccga cttaatcagt tgaagcagtg cttttccttt
     9781 ttcagtctgg ttaaaaaagt aataaagata aaaagggata tacatcaaag ctaacatgat
     9841 cgaagacagt aaatggatct gtgtcataag tcccactgag acagcgactt tgataacagg
     9901 gaacttttta tgaaggacga aatcagcaat agggatcata cataaaggat aaaaacaagt
     9961 cccccagcta gtgaatcctt ggttgataga ccagtattga atagaatacg tactcatgaa
    10021 taaaacggct actggtacac ttacccaatt tcgaacattg acatatctca gtaaaatcag
    10081 catagaaaat cctgaaataa gataaagaat gaaatttgaa agaatctgat aatgaaacca
    10141 gctacctgca agcaacacga tcaatccttg taaataagcg aaaaatgggc cgtaaacggc
    10201 attcacgatc ctgccacttt gctggaatcc ataaagggaa ggaaaataat ggaaatcccc
    10261 atatttaatt tgttgcgcgg catcatagaa ccgattgtaa tggaatgggg catcagcacc
    10321 caaaattagt ttatgtgtga ataaatgagg catgagaaat gccaaagcaa tgaaaaaaat
    10381 tgctaaaaat gggccatatt ttttcaattg tcgaactcct ttgcaaactt tagataaatt
    10441 catcatatat cctatgcgag aggtaggcaa gaaattagaa aattcatgaa aatatttatg
    10501 aattttacaa gaaaaacata agtgaaagtt ctaaggagag aggatgcgac ataagttttg
    10561 ttgcatctcc ttgttttgaa acagaatttt tcttaatact cgaaacaaag tagctttttc
    10621 atgacctctc ggtaaacggt gttcaatcag ctgcttctcg gtaataagct cactagccta
    10681 gaaaatatga gaagctgttt tctgactagc gctcttatta ctcaaagcat aacgcagttt
    10741 tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaaccc
    10801 aacaaaaaat atgagtagct atttttttgg gctgtactta ttacttgatg caggacagct
    10861 ttttcacaac ctcatcggct ttcattttct atttttttcc tgaacccata taataaaaga
    10921 aagaacaaaa aaacttgcaa gggaggacac atgtgaagca gagtaagcaa aaacagtctc
    10981 gtcgactttt gtttcgtttg tttttattaa tatgtatcgt cgtagggtta ctgatcgctt
    11041 tatatccatt ttatgtggat tctttaaata gttttattga tcaaaaacgt atcgaacaag
    11101 tacaaaaacg gaccgcagct gaaattgaag tgcagcgaaa gaaaatggag gagaaaaaac
    11161 aacgattaac tgaccaagga ttcaatcctg gtactgatcc tttcaatgaa aagaacagaa
    11221 atgaaagtac aacatccagc caattagagg aatggctgat cggaagtatc aatataccta
    11281 agatccaatt aaatatttca ctttatgatc ggctcaatgg taggatcctt gaaaatggag
    11341 caggcgtctt gcaaggaacc tctttcccgc ttggtggaaa ttcgacgcat tcagtgatct
    11401 ctgctcacag cggtttacct aatcgacggt tatttaccga attagatcgt ttagaaaacg
    11461 gagacacgtt tattttgact gttttggggg agaaattggc ttatcaagtt aaaaatatac
    11521 aagtggtact gccagatgac acgtctgttc taacgatttc agaaggagaa gacttagtta
    11581 ccttgcttac atgtacgccg tatatgatca acacgcatcg tttgctagtg acaggacatc
    11641 ggattcctta taacgaatcg gtaaaaaaag aagaaggaaa aggaaatcag gaaagaacca
    11701 tgcggcaatg gtttattctt gcaggaacga taatagctgt agtcatactg ttagtgttta
    11761 tcggacgact aatctatcag tatagattaa gtaaaaaagt gcttgatttt tcatttatta
    11821 ttagtgattc gtcagggaaa ccggtaaatg gtagttcatt cattttgaaa cataagaaaa
    11881 aaacacttac tagaaatggt gtgccgtttt cagtacaaag cgaccatcat gggaaagtca
    11941 agctggatca attgcctggt ggaacttatc gaatcgtttc agctgatgat cctaaagttg
    12001 ctgtttcttt tggtattaga aaagtaaaac aagagaagat atactttttt gaggggcgaa
    12061 aacttgtaaa agagcttcaa aaaaatggtt tttattttaa aatcaatgaa taagtaagga
    12121 gagaaaaaat gatccgcaca acaacaaaat tgtctgccaa agaactggaa tctgttctat
    12181 cgatttggct gcatgctaac ttggattctc atgaattcat tccatcaact tattgggaga
    12241 atcaagtaga atctgttagg aaagctttac cagatgcagt gatttactta gcagaaatag
    12301 atcaagaaat tattggtttt gctggggtga ttgatcagtt tatcgcagga atctttgtga
    12361 aaagggaata ccggcaacaa agaatcggta cgcaactaat gaaaaaaata aaagaaacca
    12421 ataaaacact gactttgaca gtctatcaaa aaaatcaatc agctattcat ttctatcttt
    12481 cacaaggttt tacaattcaa acacagcaaa aagatgaagc agtaaatgag gaggaacttg
    12541 tgatgaaatg gaatcaaaac gctgattttt gatgaacaga ataacgaaat aatgctccca
    12601 gcctaatttt ttgctgggag cattatttca ttttgaagtc actaatttaa ctgtgtgaat
    12661 atgcagttgt tacaatcttt gataaacggt gttcaatcag ctgaccctcg gtattagctc
    12721 aaaaaacggc aaaatatgag gaactatttt tccatttgtt tctctaatac tcaggtcaaa
    12781 acgctgattt cacaacctct tgataaacgg tgttcaaacg gctgcttctc ggtaataagc
    12841 ccactagcct agaaaatatg aaaagctatt ttctaactag cgttcttatt actcaaagca
    12901 taacgctgat ttcacaacct cttgataaac ggtgttcaat cagctgaccc tcggtattag
    12961 ctcaaaaaac ggcaaaacat gaggaactat ttttccgttt ctttctctaa tactcaggtc
    13021 agaacgctga tttcacaacc tctctttcac aaccttattt tactcgttta taattttcat
    13081 cgaagtattt tccgctacgg atatcatctg gcattccatc gtatggttct tcgctaataa
    13141 catcttcatt gtctatacct gcatcaccca tataagtgat cgtagcaaat tcaggaacaa
    13201 gtaattttgg ataagtttca tatttttcac gagctgcttc catttctgcg atatgttcgt
    13261 tttggaaagt aacagtgatt tctggatgtt cttcaaccca tttagggaaa aagatcattc
    13321 cgtgaagttt cttttcattt ggcataaagt ccaatgccac gtctgtccct gttggctctg
    13381 tccaagtgat tttgaatacg ccgtccgtca atttaacgat atcagcttct tggtctttta
    13441 cccaacggcc tttgaccatt ccgccatgga tacggtaatc aacagtatgg tcatttttgg
    13501 cataccattc atattcccaa ccattgtcat acgtatagat aaagtgagtt ccaataaaat
    13561 cttccaattc tttaaattct ttcatgttta agcatctcct tataaattaa aacatgtaat
    13621 aaactacatg ttttcgatta ctcatttatg atagaatgga tttaaagatt tgtcaaggag
    13681 ggaaaactga tggcaagaaa aaatacactt cgatatattt tgttagggtt attaagtaag
    13741 aagaagatga caggatatga attgaatcaa tcgttcaaaa atgaaatagg agaattttgg
    13801 caagcaaaac atagtcagat ttatcctgaa cttgcaaaaa tggaagagca aggaatcatt
    13861 cagcatcaag tagagatcac aggtgaaaaa ttggaaaaga aagtttatga attaacagaa
    13921 gaagggaagg aacaattggc agagtggatt catacaccaa ccaatgaact accggttaat
    13981 cgagacgaat ttgttctgaa attgtatttc gtcaaagacg tgaatgatcc ggcattagta
    14041 gaaattattc agcagcagcg tgaccttcat gaagaaaaac gacttcatct attagagcga
    14101 aaaaaaacga tctttcctac tgaaaaagaa aaacatgaaa attacggaca ctacctgatt
    14161 ttagcgcatg cgatcaatcg ggaaacagaa tatgcaaatt ggctaacaga tgtattagaa
    14221 gaaagtaacc gaaaaaaata aaaaaatatc cgaacagtga tggagaattc ctttctatat
    14281 gaaagaaacg cagatcaccg ttcggatatt cttatgttca aacaggcctt ttttcaagta
    14341 tttatgtagc atctacttat taaaaagctt ggtacttagg agattagtgg cgtttagagt
    14401 tcttttaaca actgttcgta gtcagctttt tcatcgacag gaaggagctt gtaagttaat
    14461 tcgtctgtct cgttgtcatg aatgatgtac atcttagcat tctcttcttt gaagttaccg
    14521 atttgtaaaa tcgtatagcg ttcgtcgatc aattcgatat ctttgattgc ttttgaaaaa
    14581 actttacgga catggtgcat tttcttttgg cggatttctg ccttttctgc agcttttgac
    14641 aaagtaaatc cgtcatctaa atattttttg atcagttcta cttttatgac tgtaggtaaa
    14701 cgatattttc taggacaatt cgcatcagct tcgatcgaac gaatgaatcc cttttgttcc
    14761 caatacctta attgacgtgg agacacacct acgatatcac ttaattcact gatgccaaca
    14821 accaattgat cattttccaa caaacgttta agggaagccc caaccatttt tgtcactcct
    14881 tttgtcctct tgcttccctt aattatatcc gtattaactt atgtgtcaag taggttgaca
    14941 aatagcgaat ttaggactaa agtattctag tagtttcaga aaaaattcag aaagaaggag
    15001 aaatatgagt aacaaccaac ccgtagatat ttacggaaaa ccatataatc ggacactttt
    15061 agttgttgtc ttattaattg gtacattttg taccgttttg aatcagacct tattgacgac
    15121 agcttttcct acattgatga aagctttcga tatttcagcg tctgatgtgc aatggttaac
    15181 gactggtttc ttgctagtca acgggatcat gatcccaata acagcatggc tgatcaataa
    15241 attcagttca agaaatttat atctttctgc catgacgatc tttttgatcg gaacgattac
    15301 ttgctttaca gcacctaatt ttagtacatt actaatcggt cgcttgatcc aagcttgcgg
    15361 cgtgggagta tcgatgccat tattgcaaaa tatcatgcta tctattttcc cgccagaaaa
    15421 aagaggatca gcaatgggga tgtcagggat cgttattgga cttgctcctg ctttaggccc
    15481 tacattatct ggttatatca ttgataacta ccattggcgt gacttgtttg gtatggtact
    15541 gccaatcgtt gttcttgtct tggttttagc attcttccta atgaaaagtg ttattccatt
    15601 gtcaaatcct agcattgata ttttatccgc tattttatca acgattggat tcggtagttt
    15661 actttacgga ttttctagtg taggagatga cggctgggga agcttgaaag taattggttt
    15721 cttagttgtt ggtgtgattt tcatcgcctt gttctcatgg cgtcaattac atttggaaca
    15781 tccattcttg gaacttcgtg tattcaaatc atctacattc acgattgcag ctgttttagc
    15841 tggtgtaaca aatatggcga tggtaggggc agaaatggtc gtaccattgt atattcaaaa
    15901 tattcgtgga gaatcagcat tccattctgg cttgatgttg ttgccgggtg cattgatcat
    15961 gggggctatg atgcctgtga cgggtgcgat tttcgataaa tacggcgcga aacgtttagc
    16021 catcacaggg atgttcattt tgacagcggc aactgcacca tttgctttct tgacaaaaac
    16081 gactccagtt ttatatatcg tgatccttta tgcgatccgt atgtttggga tctccatggt
    16141 catgatgccg gtaacgactt ctggtatgaa tgcattgcca aataacttga tcagtcatgg
    16201 gacagcggta aataatacat tccgtcaaat cgcaagctcg attggtacag ctattttgat
    16261 cagtgtgttg acgaatgtga caaaagcaaa tatcccaagc aaatctgtat tggataatac
    16321 gccattagct tataaagatc aggcaatcaa tgccacactg tcaggttacc atgccgcgtt
    16381 tatcgtagcg atcatctttg ggattcttgg ctttattatt gcctttttat tgaagaaaaa
    16441 agaacctgtt ctaatgaaag cgggggataa tgcatgatta ttggagtttt gattgtcagc
    16501 ttgcttgctt ttgctttaac gaacgtgttc gctaaaaaaa catggcaaac ctttttatct
    16561 ttgattttcg gactaatctt cgtcgcttca ctaagtttga tcgtcgctaa tttgtccaat
    16621 cactttggaa tggaaaaagt aacggaaaca aaaacagaaa aaattgtttc cagtgctgat
    16681 tcccaaggag cagatatgct tttgtataaa gctttaggga atggaaaaga aaaagtgtat
    16741 ctttaccgaa caaatgaaaa acaagaaaaa ccaaaagcaa ctggtacgga taacgaaaca
    16801 aataaagtag aaaagacaga cggcgatgct gaaaaagtga cgaaaactac ttattgggaa
    16861 tacaaaaacg acatgtataa attctggttc aatattgctg ataacaatca cgaatatgac
    16921 agccgagtga atacattcaa aatcccagag acatgggtag aattaagtac ggaccaagca
    16981 gccaagttag ctgaattagt gaagaaacag caaagtacga tggaaagtga agcaaaagct
    17041 tatgttcaag acggtatggt aaaagcaatg actgaaaatc caaaaatgag caaagcagag
    17101 caagaacaac gtacgaaagc cttagctgct gaattccaac aacaagcatt cgcaaaatta
    17161 gtgaaagaag caaaaggcga ataatatttt gaacaccatg taggaggttg tgaaaaagct
    17221 gctctgcatc aagcagtaag aacaatcaga taaaaatagc actccatatt tttcatctga
    17281 ttgggcttag tgccgtagat gcaagctttt gaacaccgtt tgttcggaaa aagggactgt
    17341 gacacggttt t
//
LOCUS       DACDYJ010000057        17182 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDYJ010000057 DACDYJ010000000
VERSION     DACDYJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.057"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IWM51_001979"
     CDS             complement(543..2048)
                     /locus_tag="IWM51_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9971389.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IWM51_001980"
     CDS             complement(2045..2731)
                     /locus_tag="IWM51_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9971390.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWM51_001981"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWM51_001981"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP9971391.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="IWM51_001982"
     CDS             complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="IWM51_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9971392.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4752..5321)
                     /locus_tag="IWM51_001983"
     CDS             complement(4752..5321)
                     /locus_tag="IWM51_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP9971393.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5507..6409
                     /locus_tag="IWM51_001984"
     CDS             5507..6409
                     /locus_tag="IWM51_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9971394.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="IWM51_001985"
     CDS             complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="IWM51_001985"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP9971395.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="IWM51_001986"
     CDS             complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="IWM51_001986"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP9971396.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="IWM51_001987"
     CDS             complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="IWM51_001987"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP9971397.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12977..13162
                     /locus_tag="IWM51_001988"
     CDS             12977..13162
                     /locus_tag="IWM51_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP9971398.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(13224..14420)
                     /locus_tag="IWM51_001989"
     CDS             complement(13224..14420)
                     /locus_tag="IWM51_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9971399.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14590..16026)
                     /locus_tag="IWM51_001990"
     CDS             complement(14590..16026)
                     /locus_tag="IWM51_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9971400.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            16319..17128
                     /locus_tag="IWM51_001991"
     CDS             16319..17128
                     /locus_tag="IWM51_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9971401.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         5042 a         3595 c         2983 g         5562 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attttttatt
     4441 taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt tttacatttt
     4501 ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata gtaaccacaa
     4561 tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc gtttaaccct
     4621 ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga tgttcttcta
     4681 gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat tagacacaat
     4741 atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag ttcagatagt
     4801 tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg ctggtattct
     4861 tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc ttcctcttct
     4921 tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga ctctgctaaa
     4981 ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc atctctactt
     5041 tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg cagttcaaca
     5101 ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta atgaacgata
     5161 tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac atcaagtatt
     5221 aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa agttaacggt
     5281 gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca ctcctaagca
     5341 acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt gacaccttgc
     5401 agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat catcctcaga
     5461 atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga acacgaaaga
     5521 tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg ctaattattt
     5581 ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag agtttgacac
     5641 cgtacttatt acaagagatc gatcgcatca cgaattgatc gtcacgcctt ctggaaagca
     5701 gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag ctgaaatcac
     5761 tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca atgaatactt
     5821 tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg agattaccga
     5881 tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt tagctgtatt
     5941 aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt tggacaaacg
     6001 tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg tctctttcaa
     6061 agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc ccgcagcatt
     6121 cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa gcaatgactt
     6181 gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg cagagattgc
     6241 tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc agcctcgctt
     6301 cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag aacaagtagt
     6361 ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa agaagagctg
     6421 tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta aacgatgccg
     6481 cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc gcctttagca
     6541 ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga agtcaatccg
     6601 ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc tttgtcagtt
     6661 gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc agcagatgaa
     6721 gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc taataattta
     6781 gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac tggtacacca
     6841 gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc ttttttagct
     6901 gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg gtaaacgccc
     6961 cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa gatatctgca
     7021 tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc agcaacgatt
     7081 gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt ttcagttaca
     7141 tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc attttcagct
     7201 tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc gtttgcagat
     7261 tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac gtcagaagct
     7321 tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt agcagtgata
     7381 actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat tggtactagt
     7441 tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc agaaactttg
     7501 atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa gatttgaaca
     7561 tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg tgctttacgt
     7621 acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat atcgtcagca
     7681 tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac ccctttacgt
     7741 gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc ttcgtctttt
     7801 tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc aacgtgaaca
     7861 tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac atgttcagga
     7921 tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc acgaccaaaa
     7981 tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt atctaaaaga
     8041 ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat acgtgataat
     8101 tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc agattcaatc
     8161 aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac gattttggtt
     8221 tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta cacagcttaa
     8281 atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat atagtgataa
     8341 gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa cgatttcgtt
     8401 gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga cagcgtaagc
     8461 accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac ggacaacgtg
     8521 tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct cagcgaattc
     8581 atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc cgcgatcgcg
     8641 gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg ggatgatgat
     8701 ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat tgcgtcccat
     8761 tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac ggtcaattga
     8821 ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc caggaatatc
     8881 attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca aagccattgc
     8941 gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga attttttcaa
     9001 ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac gtgcagaata
     9061 caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa gacgacgaat
     9121 atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca gtccttcgta
     9181 aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt ctccgccact
     9241 ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac tttatcctaa
     9301 gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct aacattattt
     9361 ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca agtagtattt
     9421 tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat aagttgccgc
     9481 tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt tcgttgcgtt
     9541 ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat cacttgtaat
     9601 tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca agtttgtgaa
     9661 ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag ttgcagttcc
     9721 tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc aacggcaaac
     9781 aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc tccactactg
     9841 tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc tctaacttcc
     9901 ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat cagctgaact
     9961 tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa ataaatacct
    10021 aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac ttcttcttct
    10081 tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat attttggatt
    10141 tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc aaaaactcct
    10201 actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat ccgtatgagg
    10261 aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat atcttctaca
    10321 aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc cttattgacc
    10381 aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag tttattttta
    10441 cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga attcatcagg
    10501 ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa tcccacgaat
    10561 gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc gatatattca
    10621 tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga tacaaaatgc
    10681 gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg cagaatatct
    10741 gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg ttcctgatag
    10801 acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg gtagcgaatg
    10861 gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg gcctggtcga
    10921 tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct tcgtaaaacg
    10981 ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg ttgaaacaag
    11041 agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata cccttctact
    11101 cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc taaaaagtcc
    11161 attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag aaaagcttca
    11221 tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat aactacacct
    11281 gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt tttgaataaa
    11341 agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt tttataagct
    11401 tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt tgcttcactt
    11461 tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc cattgtgcca
    11521 aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata attcaatact
    11581 tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa gcgttcgggg
    11641 tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt gatttctaac
    11701 acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac gattttgttt
    11761 ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa tcccatttga
    11821 tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata gtcagtgatt
    11881 cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg ttttccatta
    11941 ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga aatcgattca
    12001 gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa tcgtttgatc
    12061 atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc agataaattt
    12121 tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc aaaaagttct
    12181 tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc ttttatcaca
    12241 tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat ataaaaattt
    12301 ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc ctctttattg
    12361 aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc ggtttctgtc
    12421 atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt catcagctgc
    12481 tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc ttcataagta
    12541 taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat acgatagaat
    12601 tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta gccttgtttt
    12661 tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga ataagaagta
    12721 attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt taaaattcac
    12781 tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg aagttgatta
    12841 ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa gaactcaaat
    12901 tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa attaagatag
    12961 aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg ttctaggtca
    13021 agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta ctttgacaac
    13081 aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg cgccaagaaa
    13141 agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc aagctctttt
    13201 ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg aaagttgaat
    13261 aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac agcactgtaa
    13321 ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc aaagttgcta
    13381 aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat cttactgatc
    13441 agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa gcgaagtata
    13501 cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa tgataaaatg
    13561 agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc tagtttcggt
    13621 gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac aaaaaggata
    13681 ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct gatcgtagtg
    13741 attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga gagatgatcc
    13801 atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg atggaaatct
    13861 tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc tgtgatcaag
    13921 aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg tccgatcaga
    13981 tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt ttctctaggt
    14041 gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat cccattccaa
    14101 aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc cattgttaat
    14161 gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc agctagattt
    14221 ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc tagtcccgaa
    14281 aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg tatgaatggc
    14341 ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat cactaaattt
    14401 tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat acctattttc
    14461 gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag gtcattttat
    14521 atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt tccaatccct
    14581 tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa tatcaacgat
    14641 ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa ttgtatagaa
    14701 aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa agatcgatgc
    14761 aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa aagaagccat
    14821 catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta caatggcgat
    14881 cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg aagtcaaacc
    14941 agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg tattcgatgt
    15001 accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt tatccatttt
    15061 tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga aaatacctgt
    15121 gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata aactgaatgc
    15181 gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag attgcatgcc
    15241 ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat tacctaacga
    15301 attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac caaggactgt
    15361 ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta aaatggctgt
    15421 cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg ctaacgcagg
    15481 gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga cagagctcga
    15541 aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc cgacatacgc
    15601 aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa tcgcccgaat
    15661 aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc aaataaacac
    15721 cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa aagtgaaaaa
    15781 tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca atcccattac
    15841 cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga agggcatacc
    15901 tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa agaatgtcgt
    15961 tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac cgaaaaactt
    16021 ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa taataaaaaa
    16081 taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt tcattcgctt
    16141 ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt ttagcttatg
    16201 tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat tgctcttgtt
    16261 aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag gacccttatt
    16321 gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag attcgatgat
    16381 tacatctaaa acaaaagaga ctctgaaaaa agcaatcaat gccggtcatc atgtcagtat
    16441 tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag aattaacaac
    16501 accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact gggatggtga
    16561 aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa aaaagcagct
    16621 aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca gttttgattt
    16681 ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc tttctcctaa
    16741 aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt ttgcaggagc
    16801 tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga catggggtgg
    16861 acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag gcgtgcaaga
    16921 gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg acgagcataa
    16981 tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg gtactgacca
    17041 actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg gtttagctgt
    17101 ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa aagaggttgt
    17161 gacaattttt gtcacaacct tt
//
LOCUS       DACDYJ010000058        17006 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDYJ010000058 DACDYJ010000000
VERSION     DACDYJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.058"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..125)
                     /locus_tag="IWM51_001992"
     CDS             complement(<1..125)
                     /locus_tag="IWM51_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP9971402.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(229..540)
                     /locus_tag="IWM51_001993"
     CDS             complement(229..540)
                     /locus_tag="IWM51_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9971403.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(542..739)
                     /locus_tag="IWM51_001994"
     CDS             complement(542..739)
                     /locus_tag="IWM51_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP9971404.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1133..2860)
                     /locus_tag="IWM51_001995"
     CDS             complement(1133..2860)
                     /locus_tag="IWM51_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9971405.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2853..4046)
                     /locus_tag="IWM51_001996"
     CDS             complement(2853..4046)
                     /locus_tag="IWM51_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9971406.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4233..4877)
                     /locus_tag="IWM51_001997"
     CDS             complement(4233..4877)
                     /locus_tag="IWM51_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9971407.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4971..5105)
                     /locus_tag="IWM51_001998"
     CDS             complement(4971..5105)
                     /locus_tag="IWM51_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP9971408.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5107..7239)
                     /gene="feoB"
                     /locus_tag="IWM51_001999"
     CDS             complement(5107..7239)
                     /gene="feoB"
                     /locus_tag="IWM51_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP9971409.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7236..7709)
                     /locus_tag="IWM51_002000"
     CDS             complement(7236..7709)
                     /locus_tag="IWM51_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP9971410.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8110..8334
                     /gene="nrdH"
                     /locus_tag="IWM51_002001"
     CDS             8110..8334
                     /gene="nrdH"
                     /locus_tag="IWM51_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP9971411.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8493..10652
                     /gene="nrdE"
                     /locus_tag="IWM51_002002"
     CDS             8493..10652
                     /gene="nrdE"
                     /locus_tag="IWM51_002002"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP9971412.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10702..11667
                     /gene="nrdF"
                     /locus_tag="IWM51_002003"
     CDS             10702..11667
                     /gene="nrdF"
                     /locus_tag="IWM51_002003"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP9971413.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11756..12895)
                     /locus_tag="IWM51_002004"
     CDS             complement(11756..12895)
                     /locus_tag="IWM51_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9971414.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13204..13917)
                     /locus_tag="IWM51_002005"
     CDS             complement(13204..13917)
                     /locus_tag="IWM51_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP9971415.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14171..14872)
                     /gene="nagB"
                     /locus_tag="IWM51_002006"
     CDS             complement(14171..14872)
                     /gene="nagB"
                     /locus_tag="IWM51_002006"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP9971416.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15106..16638
                     /locus_tag="IWM51_002007"
     CDS             15106..16638
                     /locus_tag="IWM51_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9971417.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16829..>17006)
                     /locus_tag="IWM51_002008"
     CDS             complement(16829..>17006)
                     /locus_tag="IWM51_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9971418.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2910 g         5323 t
ORIGIN      
        1 gaaaaaacac attccactaa atttgatgcc ggttttcctt gaggttttgt tccagcacct
       61 cctttaattt ttttaagctc ttgatctgaa cattgtttag catttagtct atttttttct
      121 tccattacga ttccccctac aaactataaa tacttttatg tcactgattc aatataccta
      181 tttttctaat caaaaaattt tgagacatat ttaatattat aagtatcatt aaaattgaga
      241 tttatctcca taatctgctc gatataaacc atttattcca gcagttcttc caatttcact
      301 caaatttctt attaattcct cttgatattc agtgaatctt attttattag tataagcagt
      361 aaaatataga taattcacca atctatttat taaaggactg ggatctaatt catatttttg
      421 tttttctaat tttaaatata ccttttgtaa aacctcaaga atatcagaat atttaggatc
      481 tttacttata gatatatcat tatataattc atgaacaatt tgcttagcat tttttttcat
      541 tttagcactt ccctggaatt gctccaccta aaaaaccacc tatagacatt cctgcaatac
      601 aagtagttgc cttggcccaa tcgaccgtac atttattttt agtgcaatac actccatttc
      661 cataatattt tccactatga gtggtacccc catagataag ttgtgtctct ttaatagaca
      721 aaatttttaa atgtttcata atctaatcac ccctaaatta atgtttttag actattattc
      781 taacattcat taaatttcaa aaaacaattc cttcttgaaa cgtagcaaaa taattcctga
      841 ataagaattt aatcactttt aagtatataa actatgtata ttctttaata ttttttgaga
      901 atgcacttcg tacataataa actttaatca cattagtctt tcatacctac gatcgatctc
      961 tttgactcgc acccataaat tcataatagt ttgtcggcgt ctttgaacgt ttcgaagatg
     1021 agctacgcat tctctttttt tatagacaaa aaagatagag acgaacatgg agcttccaaa
     1081 tttggatatt tgatccaatt ttggaagctc catttatttc gtgtctgtct ttttattttt
     1141 tccagctttc gactaattct tggttctctt tgatccagtc ttttgctgct tgctgtggat
     1201 cttttccatt gttgatttcc aacataactt tttccatatc tttttctgac caatggaaat
     1261 tgtctaatac tttgtaagct tctggctggt cttctttcaa tccttttcga accatcgtat
     1321 ggatctgctc ttcgcttccc attccgtttt caggatcttc taagtatttc agatcatatt
     1381 tggcaaacat ccaatgaggt gtccatcctg taactacaat tggttcgtgt tttttgatcg
     1441 cttgacctag tgcaacggtc attgctcctg aagaagatgt ctctactttc cagtctttca
     1501 agttgtcata tttttggatc gtgttttcag cagccgtaac tacaccagct cctggctcga
     1561 ttcctatgat tttcttacct gcctgatcgg ttaactcatc gatcgaattt acatccatgt
     1621 aggctggcac tgctaaacca acttttgctc ctgtcaaatt ggcacctagg tcttcgactt
     1681 gatctttata ttgtgcatat tgtgaaccat gagttttagg tagccaagct gaaaccatgg
     1741 catctgactc gccatttgaa acagatttcc acatgattga attatctaat ggcgtcatcg
     1801 ttacatcata gcccatttgt ttcaatactt caccgactac atttgttgaa gcaacttctg
     1861 tatcccattc aacataagat aagttgatac gttttgtttc tttggcagat gaaaaactcc
     1921 ctataccaat cgcacctaag atcactacta ctacagcacc aatgatcagt ccttgacgtt
     1981 ttttactttt ctgtgttgtt gctcctgcag cttttttatt gtttggctgg ttcaaatgct
     2041 gtgtaaaacg gtcaatgatg attgctaaga taactaatgc aaccccgttg acaaatccat
     2101 tcccgacttg tgctctttgc aatgctgata atacgccgcg tcctaagccg ggtgcaccga
     2161 tcatagaggc aataacgacc attgaaagtg acagcattgt tgtttgattg atccctgcca
     2221 tgatcgtact tttagctaaa ggcaattcta gtttgaatag tttttgacga cttgtgctcc
     2281 cgaatgaatc agaagcttct actaattctt taggaacttg tcgaatccct aaattcgtaa
     2341 aacgaacggt tggtggcaga gcaaagatga ctgaggcaaa tacccctgga accatcccga
     2401 taccgaaaaa tgcaactgct ggaatcaaat aaacgaatcc tggcattgtc tgcataaagt
     2461 caaggatcgg tttgataata ctttgggctt tactgctttt tgccatcaag atccctaatg
     2521 gtaccccaat gatgatcgag atcacactag ataaaagaac aagtgtgaca gtattcatca
     2581 aatcattcca tagaccttgg ttataaataa accatagacc gattaatgtg aacaacgtaa
     2641 gtcctggacg tttcttagaa ataaagaaag ctgctaccgt caataacaag ataaacaaag
     2701 gagctggaat cactaacagt aaatttgtaa tccccgacat cactgcctgt ccaatcgttt
     2761 gtaaaaatga aaataggcca gaaaaagttg tcgtaaacca ttctgtgata ttttctaccc
     2821 agtttgccac tggtaattga tagttcaata gattattcat gttcactcac ctcgcttgtt
     2881 tctgctaatg cttcaatcac actacctcgg atgacgacac cttttaattt tccatcctct
     2941 acaactgcaa gtggagtcgg tgagtcataa ataatattga aaatatcact taccaccatg
     3001 tctttcccaa tcaccgtcac attttcatct aggaagtcga tcaatggacg tttttgcttc
     3061 ctagcttcta acgcttgatc tgcagtgatg atccctttca ggtgtctctt tctgtcaaca
     3121 gccatcagca tgctgacttc ttcattacgc atccttgtca atgcaacgtt tggtccatca
     3181 gattcgatat tcgtcgtcaa tgcaggtacc atgatgtttt gagccgtaag cactttggaa
     3241 cgatcgactt cttccacaaa ttcacgtaca taatcatttg ctggattggt caatatttct
     3301 tcacctgttc cgatctgcat gatttcgccg tctttcatca aggcgattct gtccccaatc
     3361 cgtaaagctt cattcaaatc atgggtaatg aaaataatcg ttttttgaac attggcttgc
     3421 aggtccagta attcgtcttg catttctcgc cggatcagcg gatcaagagc cgagaacgct
     3481 tcgtccatca gcaagatctc aggatcattt gctaaagctc tggctaggcc aacccgttgt
     3541 tgcatcccac cagataactg actaggatat tggtctttaa atgaaagcaa actagaattt
     3601 tctaatgctt tttctgcttt ttcttgtcgt tcttctttag gtacaccacg gacttctaac
     3661 ccgtattctg tattttccaa gatcgttcga tgtggaaaca gaccgaagtt ttggaaaacc
     3721 atattgattt tatgacgacg gacttctcgc aattcttctt tgcttaactt cgatacatct
     3781 tctccatcaa tatagatact gcccgaagtt ggctcaatca gacgattcaa tagacggatc
     3841 aatgtggatt tcccactccc ggaaagcccc atgatcacga agatttctcc ttcttttacg
     3901 tcaaaattca cgtcatacac accaaccgtt gcaccggttt tctttaaaat ctccgttttc
     3961 ggctggtgat cattcatcat atctaaagcg gcttgtgtct ttttaccaaa tatctttgtt
     4021 aaatgtttta cttctacttt taccaaacaa attctccttt tttatgtaaa ttcatttttc
     4081 tttctgaaaa aagtgaccac actatgttaa catcatgtag tcactttgtc aaaaatgaaa
     4141 gaaatttgct tgtccaaact gaaaaaaact tggctaaatc tttgtaaata aaaggtttag
     4201 ccaagttaat atttttttaa tatttcacac aatcattctg caagctgatg ctccgggtaa
     4261 aaaaagtttg tcatacgctg tagtgtatat tcatttccaa taaaatgaac gatgtcccct
     4321 gccgtaaatt tagcataggg gccaggtgaa acaagcagtt cttcattatg agaaattgcg
     4381 ataactgtgg cgccagtcgc ctgccagata ttcaactcgc taattgaacg gtccagatgc
     4441 attgcttttt ctgataaaga taattcaaac ggaagtaaag gattcatctt atgtattcgc
     4501 ttcgtttgat tgaccagctg atccaataat tgagaaaaat gattcagctc atcttgttgc
     4561 ttctccacac ttgccatgat ttctgctttt gtatcttgga tggattgtac atcttgatat
     4621 tgcaatagga aatcttttgc tttttccttc gaagcaacaa atgccccact tccatgccgt
     4681 acttccatga ttccaagatc taccagcaca ttgatagcct tgcgggtagt ctcaggagaa
     4741 gcattgtacg tactggcaag tgttgagcgg gcatggatct tctcacccac tttatatcgg
     4801 ttttcaacga tacgttcggc taaatcaaca gcaatctgct ggtagcgcgg gcggacgatt
     4861 tttttctttc cagacatttt ctttcctctt tcttttttta ctttccctca tcttactatg
     4921 ctctagcaaa ataagcaaaa aaggggagaa tagcctcttg caacgactga tcatcgcttg
     4981 atctgttcct tttttactgg gcaagctgtt tgacagtcat cacaattctt accggatttt
     5041 aaacgagtat agacaattgc gcctgcactt ccaaagatta acacacttaa taagaatgtc
     5101 gccatcctat tcctctcctt ctaatgacaa tatagtataa aactggttct tggattttgg
     5161 ctgacggaac aaaacataaa cgagtcctat catcagtaaa atagcaagat acgtccctaa
     5221 cgctacctgt ccaccctcca ataagtgtcc caactgataa atcacaaaac taactaaata
     5281 agctaaaccg cattgatagc ctatggcgat ccaagtccat ttcttggcat tcatttcccg
     5341 atggatagcc ccaattgccg caaaacaagg cgcacaaagt aaattgaagg ctaacaatga
     5401 ataagctgct gcgggagtat atgtgtgttg tagcacttgc cagatttctc tgccattttc
     5461 ggatacttcc gcatgaccaa acaaaatacc aaatgtgcca ataatatttt ctttagcgac
     5521 caatcctgta atcgctgcga ctgttccttg ccagtttccc caaccaagcg gctgaaaaac
     5581 aactgccagg attcgcccga aaaaggctaa gatactttgg tcttcagaaa caggttgagc
     5641 ataaaaatta taagaagagc tgaaccagat cacgatacta gaaacaaaga taatcgttcc
     5701 tgctttttta acaaaagact tgctgcggtc ataggtttgt cttaggaccg ctgaccattg
     5761 aggaaaatga tatgcgggca attccatgat aaaaggagct gtatctccag caaacaaacg
     5821 tgtttttttc aagctgattc cagagaaaat aattgcagtc ataccaagaa aataggcaga
     5881 aggagctacc caagattgat gaggaaaaaa ggcccctgct actaaggcaa taatcggcaa
     5941 ctttgcagaa caaggcatga aggttgtgac catgatcgtc aaacgacgat cttgctcgtt
     6001 ttcaatcgtg cgactggcca tgacgcctgg cactccacat cctgtagcaa tcagcatagg
     6061 aatgaaggat tttcctgaaa gaccgaattt cctgaaaagt cgatccatga caaaagcgat
     6121 cctcgccata tatccgcagt cttccagaaa gctcaagcat aaaaacaata taatcaattg
     6181 cggtagaaat cctaacactg ctcccacgcc tgcgatgatg ccgttcaaga tcagctcctg
     6241 catccatccc gctacttgcc attcagctaa caatctgcca acatgttctg gtaaccatgt
     6301 accaaacaat gtgtcattga tccaatctgt ccccattgtt cctactgttt gaatagccag
     6361 ataatagatc agccacatca caaatgcgaa gatcggcaag gctaaccaac gattcgttgc
     6421 aatctgatcg attttgtcac tcatcgataa ttggaaagaa tcatttgaca caacacagag
     6481 tgtacatagt tgggtaataa attcataacg ttcattgacc agaatcgtct cactatcgtc
     6541 gtccaatagt ttctctgtta actggatcag ttgctcgatc tctttttgct gagaaactgc
     6601 tatctcgtaa tcctctctta ccttctgatc tcgttcaaaa tatttcattg cataccagcg
     6661 tttcttgccg ctttgttctc ctagtacatc ttcaatttca tgtaaagctg cttctaaacg
     6721 tggatcatat gtacagggaa caggaataga aggccgcttt tttgcagact cctgaatctt
     6781 ttcgattagt ttttcgattc cttgcttttt caatacgctg attggtaaaa ctggtacacc
     6841 aagaccatag gaaagctttt ctagattgat tttttttcct tcttttttga tgacatccat
     6901 catattcaga ccaatgatca taggaatgtc taaatccaat aactgcgtag ttaaataaag
     6961 atttcgttct aaattggtag catcaataat gttgatgatc agttcaggct gttcctgaac
     7021 caaataatct cttgcaatca cttcttctgg tgtataagga gacaaagaat agatgccggg
     7081 taggtcctga atatgaatgt tcggattttt tttcaacgat ccttcttttt tttctactgt
     7141 tacgcccggc caattcccta catattgatt cgaaccagtc aagagattga acaagctagt
     7201 tttcccgctg tttggatttc cgactaacgc gatattcatt ctttttcctc cctttgtatc
     7261 aagattagtt cggcctctga ctttcgtaac gtcagttcat accctcgcac tgtcagttcg
     7321 attggatctc ccagaggggc tagttttcgg ataactattt ttacatttcg cgtcaatccc
     7381 atatccatta accgtcgctt cactgcgcct tgtccgtgga ttgctacgac gatcccagtc
     7441 tctcctgctt ttaattgatc aagagaagtc cagctttcag aggaagcagt ctttttattg
     7501 acaaagattt gacctagtat ttcccgactt actgccactc gatgactttt taccagtaaa
     7561 atggcattgt tgcctgataa attgacgacg acgattttac ttccggcaac gatcccgata
     7621 ttttgcaaat ggcgttttgt tttttcttct gtttcgattc gttcgacttg ataaacgaaa
     7681 ttctgctttg cctgcgctaa ggtctgcata ttgattcctc tcttgtgaaa taaatcatgt
     7741 attaaaatca ttcattatat gttctaattt tcttacgtgt tacgcaaata aatgagaatc
     7801 attctcacat agaatactct cggtaattga gaatgtcaat tatacttgtg atattatgag
     7861 cgaatcttta gtattgatag ataaaaatga gaaaaaaata tttcttcata gttttccaca
     7921 attttttctc tattaaattt gctattttcc cacacgatac ttgttttcag ctcttttttc
     7981 aaaaacacaa aacttagtat tccacagttt ccacaacaca atatgtagtg tttgttgttt
     8041 gactttttta ctaagagttt tatactatct attatagata ataagaatgt gttagaggag
     8101 gcactcacca tgaacgtaaa aatcttttct aaaaataatt gtatgcaatg taagatggtc
     8161 aaacggtttt taacagaaaa tcaaattgct tttgaagaag tcaatatcga tgaacagcct
     8221 gaagcagtag actggttgaa agcacaagga ttccaaagtg ttcctgtcat tacttctgat
     8281 gcagaaacag ttatcggatt ccgtcctgat cagttgcgta aacttgccag ctaacatgac
     8341 taaaagaatc aaaacgtgat acatggattt gaagggcttg agacagcacc agtttttgtc
     8401 gcaaacttcc aaatcctttt ttaaagctta aaaacaatgc aatgatttgc aatcaagcat
     8461 catcaaatac gaaatgaagg ttgggcgtac gtatgagtct taaaaattta caagatgtca
     8521 gttatttcaa gctgaataat gaaatcaatc ggccagtcaa tggtcaaatc cctttgaata
     8581 aggataaaga agctttagtt gccttttttg aggaaaatgt caaaccaaat acgatgactt
     8641 ttccatctat tatggaaaaa atcaattact tgatcgatca agaatatata gaagcagaat
     8701 tcattcaatt gtattcacca tcatttattg aaaagctata tcaatttttg gacgagcaac
     8761 atttttcttt caaatctttt atggctgctt acaaatttta ttctcaatat gcgttgaaaa
     8821 ccaacgacgg ttccgcttat ctggaaactt atgaagatcg agtagccttc aatgcgcttt
     8881 actttgcgaa cggggatgaa gaattagcac ttgctcttgc agacgaaatg atccatcaac
     8941 gatatcagcc agctacacct tctttcttga atgctggacg taaacgtcgg ggcgaattgg
     9001 tgtcttgttt cttgactcaa gtcactgacg atatgaactc tatcgggcgc tcaatcaact
     9061 ctgctttgca attgtcccgt attggtggcg gcgtagggat tactttatca aatcttcgtg
     9121 aagccggcgc tcctatcaaa ggatatgagg gtgctgcaag tggtgtcgtt ccagtcatga
     9181 aattattcga agatagtttc agctattcta atcaattagg tcagcgtcaa ggagctggcg
     9241 tggtttattt gaacgttttc catccagaca ttattgcttt cctttcagca aaaaaagaaa
     9301 atgcagatga aaagattcgc gtaaaaactc tttctcttgg cgtgatcgta cctgataaat
     9361 tttatgaatt gacacgtaaa aacgaagata tgtatttatt tagtccatat agtgtcgaaa
     9421 aagaatatgg tgtcccattc tcttatgtaa atattacagc tgagtatgat catctagttg
     9481 caaatccaaa cattcgcaaa caaaaaatca aagcgcgtga tcttgaaaat gaaatctcaa
     9541 aattacaaca agaatctggc tatccttacg taatcaatat tgatacagcg aaccgcgaga
     9601 atccaatcga tggcaagatc atcatgagta atttatgttc agagatttta caagttcaaa
     9661 cgccttctgt gattaacggc aaacaggaat atgaagtatt aggaacagat atctcatgta
     9721 acttaggctc gacaaatatc gtgaatttga tgcaaagccc taactttggt caatctgtgc
     9781 gtacgatgac tcgtgcattg acatttatta cagatgcatc tgatatcgat gtcgtaccac
     9841 cgatccaaaa cggaaataag ctgaatcata cgattggatt aggtgccatg ggcttacaca
     9901 cttatcttgc aaaagaacaa atggaatatg gctcagagga ttcattagac tttacaaata
     9961 tttacttcat gctgttgaat tattggacac ttgttgaaag caacaatatc gctcgtgaaa
    10021 gaaaacaagt tttccataat tttgaaaaat cagcttatgc tgacggcact tatttcgaaa
    10081 aatacgtaac tggagaattc cagccaaaat cagaaaaagt caaagcattg tttgaaggca
    10141 tttttatccc aactgctgaa gattggaatg ctttgaaaca agcagtaatg aaagatggac
    10201 tgtatcatca aaatcgcttg gcagtagcac caaatggctc aatttcatat atcaacgata
    10261 cgagtgcaag tattcatcct atcactcgca tgatcgaaga acgccaagag aagaaaatcg
    10321 gtaaaattta ttacccagcg ccttatctat ccaatgagac gattccgtat tacacttctg
    10381 cttatgatat ggacatgcgt aaagtaatcg atgtttatgc aacggcacaa caacacgtcg
    10441 atcaaggcat gagtttgact ttattcatgc gttctgaaat cccagaaggc atttatgaat
    10501 ggaagacaac aacgaagcaa acgacacgtg atttgaatat tttaagacat tacgctttcc
    10561 acaaaggaat caaatccatt tattacgtac gtacctttac tgatgatgca gaagaaatcg
    10621 gaagcaatca atgtgaaagc tgcgtgatct aatatcatag atagattcgc ttattcatat
    10681 tttaattaaa ggagcattct catgtcagaa acatattatg aagcaataaa ctggaatgaa
    10741 attgaagata tcattgataa atccacttgg gagaagctga cagagcaatt ttggctagat
    10801 acacgtatcc cactttcaaa tgacctagat gattggcgta cattgtctca attggaaaaa
    10861 gatacagtag gacatgtatt tggcggtttg acactgctag atactgtcca atctgaaagt
    10921 ggcatggatc agttgcgtaa agatgtccgc actccccatg aagaagccgt tttgaacaat
    10981 attcaattca tggaatccgt tcatgccaaa agttattctt ctatttttag tacactgaat
    11041 acgaaaaaag aaatcgaaga aatttttgac tggacaaata cgaatcctta tctgcaaaaa
    11101 aaagctgagc gtatcaatga aatctacaaa cacggtacac cgctagaaaa gaaaattgcc
    11161 agcgtctttt tagaaacatt cttgttctat tcaggttttt atactcctct ttattattta
    11221 ggaaacaaca aattggctaa cgtagcagag atcatcaaac taatcatccg agatgaatct
    11281 gtacatggaa cctacatcgg ttacaaattc caattaggat tcaacgagtt acctgaagca
    11341 gaacaagaaa cgttaaaaga ctggatgtac aatcttcttt atgagcttta cgaaaacgaa
    11401 gaacgttata cagaagaatt atacgatcca atcggctgga cagaagaagt caagacattc
    11461 ctacgctata atgccaacaa agcattgatg aacctaggga tggatccact ctttccagat
    11521 actgccaatg atgtcaaccc aatcgtaatg aacgggattt caacaggcac aagcaaccat
    11581 gacttcttct cacaagtcgg aaacggctat ttgttaggtc atgtagaagc gatgaaagac
    11641 gacgattatt tgattggatt ggaataagga tttattttaa ttcaaaaatt tttcgaaaaa
    11701 taaaaagagt ttagcgtaca gatattttaa atgtacactg aactcttttt tatgcttatt
    11761 cacttgtttc ttccagcact tttggactgt ctaacttttc cgaaacatgt tccattcttc
    11821 tcaataaata gattttccgg atatttaata ttatcgtctt aatcactgca tagacaggta
    11881 tgcaaatcag catgcctaat attcctgcga cacttcctgc tccaatcaat accaagatga
    11941 tcgtcaaagg atggatattc attgatttcc caaataacaa cggcttgatc aaatttccat
    12001 ctaattgttg gatcactaaa atcgacaacg ccatatacaa agcttgaaat ggagagataa
    12061 acaaaccgac aatgattgct ggtaccgctc caataaaagg gccgacatac gggataatat
    12121 tcgtgattcc gcaaatgacc gcaagcaaca agctataagg ctgatcgaat attctcattg
    12181 ccaaaaagct cattacacca ataatcaaag catctaagat cgtactgctg atataggcag
    12241 ataatgtttc gtttaattct tttaccgttt ggcgtaactc tttacgaatc gctttcggaa
    12301 agaagtgaga caacgcatca ataaatttgt gcccatcctt gaacataaat agcaaaataa
    12361 atggaacggt aaataacaaa ataaaaaatt gcatcataaa tgaaatgatt tttgagacac
    12421 ttgcagataa actgactaat atgatattga acaaattact aagagataaa tttgctgatg
    12481 ctaattgttc ttccaaattg aaatttttaa actcttcccg ctgcgccaat tcatttaacc
    12541 atttgcttgt ttcatctgca tacattggca agctctgggt caattgaatt gtctgttcca
    12601 ttaactgagg cactacattc atgacagtta aaacaactaa aatgattacg accaaaaacg
    12661 tcaataaaaa accgaacact ctcgggatcc gatgcttttc gagaaaaaca acgattggat
    12721 cgaacatata atagataaaa ccagcaacaa gcaatggtaa aaaaattgcc gaaagcattt
    12781 tcaatattgg actaaaaata ttcggcattt gtaaaataaa gtacacgccg atgatcgtga
    12841 gtactagctc cactgtccag aaaaataatt ttgattgttt aaaacgtgat tgcatacatc
    12901 tcctccaagg tcctttgata cttctattct atcatttaac aaagagttac gcgctgagat
    12961 tttcatttgc ttaaaaaaaa caaaaaaaat aaacatattt taatgagtag acgagtactt
    13021 aaacaaaaac acggtttgga gtagtgacaa aaattatact gcagtccctt tttccaaata
    13081 aacggtgttc aaaagcttgc atcttcgtaa taagcccagc caaacgaaaa atataaggaa
    13141 ctatttttat ttggctgggc ttattacttg atgtaggaca tctattttac aacctcagat
    13201 catttattgc gcaatactga ctaaatgcaa ctttgtgata tttttgtgta cggacaactc
    13261 ctcgatcaca tcggcataag tgagcccttt tggaagatca atcgtataga tatttttgta
    13321 cactcggtag tcatcaacga attttacgtc aaaattcaca tcttcgatta cgatattttt
    13381 ctcttcaaaa taatcggtaa gaaaatcttt ggtttctttt ctatggataa attggatttc
    13441 taacttttta gtcgtaggta catggatcac gcgtttcact actgtgagtg caatagcaat
    13501 cccgataaaa ctgatgatcg caatggaata gtaacccatg ccgatggcga tccctaatcc
    13561 agcaattgcc cataaagaag cggcagtcgt aagtcccgtc accgattgtt tcgtgacgat
    13621 gatcgttccc gcgcctaaga aaccgacacc actcaccact tgagcaatca gccttgcttc
    13681 atctgaacga atcgttccag caagctgtgg atactctaaa gcatctctta atgcaccact
    13741 agcgatctca acttggatca atgcaatgat acatgctccc atacaaacta aaatatgcgt
    13801 acgcatacct gctggacgat ttttatattg tcgttcaaaa ccgattgctc cgccaaatag
    13861 catagcgatc cctaaacgta aaataatctc tggaattgtc aataccatat cattcatcta
    13921 aacaccctcc agtccaatat atcatgtctg aaaggagcag acaaaaaaag tgcaattttt
    13981 acctcttacg aaaaaaggac tgtgacaaaa tttttgtcat agtccttttt atgaataaac
    14041 ggtgttcaat cagctgaccc tcggtattac tcaacaaacg gcaaaatatg gggagctatt
    14101 tttcctttgt cttctctaat actcaggtca aaacgctgat ttcacaacct ctttagccta
    14161 ctattgaaaa ttacaactta cttgctgctt cttcatcgat gatcacaaca acattgtcgt
    14221 gattttgcaa tacacttgct ggcaaatgat tcgttacagg tccttcaatc atccctttga
    14281 ttgcatcagc tttgctttcg ccaaatgcca tcaagacgat ttctttactt ttcataattg
    14341 aaccgattcc cattgaaaca gcttttgttg gtacatcttc tgctttatcg aagaatcgtt
    14401 tgtttgcatt gattgttgat tctgttaagt caactactga agtcgttccg tcaaatgaag
    14461 caccaggttc attgaaaccg atatgtccat tttgcccgat tcctaagatt tggatatcga
    14521 ttggatgtgc atcgatgatt tgatcatact ttttgcatgc tgcttccaga tcttctgctt
    14581 ttccatctgg tacaaacgtt tctttgaatg gttttttatc aaataattgg acatccatga
    14641 aatgacggta gctttgttca tctgaaccgc ctagtccaac gtactcatct aagtttacag
    14701 aagtcatttc actgaaatcc aaatcgcttg aagtcatttc tttatataaa gtaactggtg
    14761 tgcttcctgt tgccaatcct aatacttttg cacctttgtt cataccttct ttgatgattt
    14821 caaatgcttt ttttccgcct tcttcagcgt tttttacacg aatgatttcc atagtgattg
    14881 ctcctttata ttttggtata gcccaattat agcattcttt ttttcttttt acaataatta
    14941 gtatagacca aattgcattt ttttcatttt cttgacaata aataggaatc cgtgttaggt
    15001 ttcataagat ggttcaatca aacaatccat gttgcatgaa aaccaagcaa gtgattcact
    15061 gtggacgctt tatgtctgca gtttttttat gaaaaaggag ttattatgag tatactgaca
    15121 ttaaacaaca ttacccagca atttggcgac aaaatcctct atgaaggtgt cgatctgcaa
    15181 ttaaatgctg gcgaacatct tgggctgatt ggacaaaacg gagccggtaa aagtacattg
    15241 attaaaatca tcactggtga gatcttacca gatgacggac agatccattg gcaaaaaaat
    15301 attcacaaag gttacctcga tcaatatgtg gaagtcgatg aaacattaac aatcgaagaa
    15361 tttctaaaaa ctgcgtacgc aaaagaatac gaaaaggaag aaaaaattgc ttttctttac
    15421 caaacatata gcgagacctt cgatgattcc ttgctggaaa aagctgggaa actccaaacg
    15481 gaattagacc aaggagtgtt ttatcaaatc gacaccttag ttgctgagat gagcagcggg
    15541 ttggggatcg atgtcttagg tttagattca ctgttgaaaa atctaagtgg tggacaacgt
    15601 tcaaaggtga ttttagcaaa actactttta gaaaacccag atgttctgat cttagatgaa
    15661 ccaaccaatc acttagatga tcagcatatc caatggctga cgcagtttct tcaggatttt
    15721 gacggtgcgt atatcgtgat ttcccatgac caagaatttc ttgatcagat caccacccat
    15781 atcgcagaca tcgagtttgg aaagattact aagtatactg gccatctaaa acaagcattg
    15841 aaacaaaagg aacaaaatcg cgaaagctat ttgcgccaat atcatgctca acaaaaacaa
    15901 atcgaaaaga ccgaagctta tattcgaaaa tacaaagccg gttcccgatc aacgatggca
    15961 aaaagccgac aaaaacaatt agataaaatc gaacggctga cgcctccagc ttcagcaagt
    16021 aaagctgtct ttgattttcc attctcgcct attgtcacga cattagcagt ggaaacaact
    16081 gatttagtca tcggatatga tcgaccgcta cttgcaccca tcaatttgac catccgcttt
    16141 ggtgaaaata ttgccattcg aggcttcaac gggattggaa aatctacttt attaaaaaca
    16201 ctgatcggtg agattgaaaa aataagtggc gatttccact tcccagataa cacaaaaatc
    16261 aattactttt cacaagatct gtactgggaa aatccattag aaacaccact acaatattta
    16321 agcagtcaat ttccaaaagc gactatcaaa gaattacgtc gccaactagc taaagcaggg
    16381 ttagttaatc aattagcaag tgaacctctc actacattaa gtggtgggga acaaactaag
    16441 gtgaaacttg cacagttgac aatgaaccaa ggtaatttac tgattctcga tgaaccgacg
    16501 aaccatatcg atcaagaaac gaaagaaagc ttacaagacg ggatacagaa atttccagga
    16561 acagtaatca tcgtctctca tgaacaagaa ttttatcaag atcttgtgca acgagtcatt
    16621 gagattgaac caaaataaac gtttgattga atagttgaaa aaaaggagct gtgacaaaag
    16681 ttttgtcaca actccttctt ctgaataaat ggtggccaat tagctgacct tcggtattag
    16741 ctcaaaaaac ggcaaaatat gaggagctat ttttatttgg ctattcttat tacttgatgc
    16801 agaacagctt ttcacaacct cttttcgtct attttttatt ttcgagattt ctctttcctt
    16861 taaaaacaac tgtttgaaag aagaacgcac atccttgaaa aacaagcccg ctaaccgtta
    16921 cacctaacca gccatactgc tgccacattg tcgtccctag caatgaacca gccgcacctc
    16981 caataaagta cataaacata aataca
//
LOCUS       DACDYJ010000059        16584 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDYJ010000059 DACDYJ010000000
VERSION     DACDYJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.059"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            59..174
                     /gene="rrf"
                     /locus_tag="IWM51_002009"
     rRNA            59..174
                     /gene="rrf"
                     /locus_tag="IWM51_002009"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(227..697)
                     /locus_tag="IWM51_002010"
     CDS             complement(227..697)
                     /locus_tag="IWM51_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP9971419.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(856..1314)
                     /locus_tag="IWM51_002011"
     CDS             complement(856..1314)
                     /locus_tag="IWM51_002011"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP9971420.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1485..2630)
                     /locus_tag="IWM51_002012"
     CDS             complement(1485..2630)
                     /locus_tag="IWM51_002012"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP9971421.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2643..3560)
                     /locus_tag="IWM51_002013"
     CDS             complement(2643..3560)
                     /locus_tag="IWM51_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP9971422.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3988..4551
                     /gene="efp"
                     /locus_tag="IWM51_002014"
     CDS             3988..4551
                     /gene="efp"
                     /locus_tag="IWM51_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP9971423.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            5037..6296
                     /gene="lysA"
                     /locus_tag="IWM51_002015"
     CDS             5037..6296
                     /gene="lysA"
                     /locus_tag="IWM51_002015"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP9971424.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6283..7083
                     /gene="dapF"
                     /locus_tag="IWM51_002016"
     CDS             6283..7083
                     /gene="dapF"
                     /locus_tag="IWM51_002016"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP9971425.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            7086..7967
                     /locus_tag="IWM51_002017"
     CDS             7086..7967
                     /locus_tag="IWM51_002017"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP9971426.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7964..8680
                     /locus_tag="IWM51_002018"
     CDS             7964..8680
                     /locus_tag="IWM51_002018"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP9971427.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8686..9381
                     /gene="dapD"
                     /locus_tag="IWM51_002019"
     CDS             8686..9381
                     /gene="dapD"
                     /locus_tag="IWM51_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP9971428.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9397..10398
                     /locus_tag="IWM51_002020"
     CDS             9397..10398
                     /locus_tag="IWM51_002020"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP9971429.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10395..11756
                     /locus_tag="IWM51_002021"
     CDS             10395..11756
                     /locus_tag="IWM51_002021"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP9971430.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11716..12867
                     /locus_tag="IWM51_002022"
     CDS             11716..12867
                     /locus_tag="IWM51_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP9971431.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12842..14011
                     /locus_tag="IWM51_002023"
     CDS             12842..14011
                     /locus_tag="IWM51_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9971432.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14186..15565
                     /locus_tag="IWM51_002024"
     CDS             14186..15565
                     /locus_tag="IWM51_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9971433.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15846..16584
                     /locus_tag="IWM51_002025"
                     /pseudo
     CDS             15846..16584
                     /locus_tag="IWM51_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         5430 a         2931 c         3528 g         4695 t
ORIGIN      
        1 tgacttcaaa tccagttttg agtgaacaaa atttactcaa taaaagataa caccacagtg
       61 tggtggcgat agcgagaagg atacacctgt aaccatgccg aacacagaag ttaagcttct
      121 tagcgccgat tgtagtgagg ggttgcccct tgtgagagta ggacgtcgcc acgcaaaggc
      181 aaaaagatga actagaaata gttcatcttt ttttattttt attaagttaa agaacggatt
      241 caattgcagc ggtcattttc aatggagaag tttgtggtgc aaatcgttcg attactttgc
      301 catttctatc aatcaaaaat ttagtaaaat tccattttat tgctttagtg acagtaccag
      361 gagcttcatt tttaagataa gtaaaaagag gatctgtatt ttctccattt acgtcaatca
      421 attgatgcat agggaaggta acgccataag tggttttaca agcttctgct gcttcagtcg
      481 ctgaatctag ctcttgttta aattgattgg aaggaaagcc taagataacc agaccttgtg
      541 tctgataagc ttggtaaagt tcttctaatt gtttaaattg aggtgctaga ccgcattttg
      601 ttgcagtgtt aacaatgatt atcggtttgc ctgcatattt tttcaatgaa tagacagtgc
      661 cgtcttctaa agtagtttca aaattataaa tagacataaa gttttctcct ttctatttta
      721 tataataaaa agagcgctaa actgaaaact agctatttgt tttcccgttt agaactcttt
      781 tcagacaata aatatttaat aaaatacgaa gataagatag ctatattgct tctcatcttt
      841 acgttctttt agaaactatg cttctacttc agaccattca tcgcgttttg ctaaaaattc
      901 acgagccaaa gcttgtgcac cttctagtgt atggctggca gcccagccac attgtgtttc
      961 attgcttgca ggtacttcag tagcttcaag aacatctttc attgttgctt caagaacatc
     1021 taaaatgtct tcgtagcttt catgattcaa tacagtcaag taaaagcctg tttggcatcc
     1081 cattggagac cagtctacga tgtaatctgc gtgattgcgg attagttcgg cagtcaagtg
     1141 ctcaagtgaa tgtagacttg gcatgtccat gtgttcttta tttggttgtt taaagcggac
     1201 gtcgtattta atgatgacat cgccatgcgc acccgttttt cgatcagcta cacgtacata
     1261 aggtgctttg acttttgtat ggtcaagatt aaaactttcg acattcattc tcatttgtaa
     1321 gactcctatc tgtttgttca ttcgttttga tgcttttcac taaagaatta tttagtgaaa
     1381 tttacaaaaa cgtctttcta cttgattcta acacttatcg tttccaaggt gtagcaatct
     1441 gaaatatttt ttagattttt ctgctaagtc gtttattggg acttctagtt cttgattctg
     1501 tcaaatgcct gagctaaatc gtttatgaga tcctgaccat actcaattcc tacagaaaga
     1561 cggatcaagc cgtcctttat gccagcctct tctcgttttt cttttggaat agacgcatga
     1621 gtcatgacag aaggaatttc cactaaactt tctacgccac ctaaactttc tgctaaagta
     1681 aatagttgga gtgattcaac aaatggaata gcagattctt cattttttaa agtaaaagaa
     1741 atcattccgc taaagccgct catctgctgc ttagcaactc catggagttc atgatcgggt
     1801 aatcctggat agtagacctt ttctactgct ggatgagaaa atagaaaatc agctaccaca
     1861 aaagcatttt tttgatgttc ctccatacga acacttagtg tcttgattcc gcgctgaagt
     1921 aaccagctat cttggggacc aagtacggct ccgatagcat tttggtaaaa tccgatctga
     1981 tcggctagat ctttatggtt agaagtcact aaaccagcta ctacatcgct gtgtccgcct
     2041 agatatttgg taccgctatg gacaacgata tcagaaccga gatctaaagg tctttgaaaa
     2101 taaggagtag caaatgtatt gtcagcaata actatcaatc catgttgatg agccagagtg
     2161 gcactttttt ctaaatcagt aatctttaga agcggattgc taggtgtttc aagataaagt
     2221 gcttttgtat tgggtttgat ggattgttct attttatcaa gattactcgt atctatgatc
     2281 gtgtactcga gaccattttt agtaagaact ttgtcaaata gacggaatgt tcctccatag
     2341 acatcatctc ctaataagat atgatcgcca gcttgaaaaa gagaaaaaac ggcatgaatc
     2401 ccagaaagac cagaagaaaa agcgaatcct cgaacaccgc cttccaaatc agcaatcagt
     2461 tcttccaatg caaaacgagt aggattacca gatcttgaat attcatattg tttaggctgg
     2521 ccgacgccat cttgccgata agtggatgtt tgataaatag gtacactaac agctcctgtt
     2581 gtgggatctt cactgatgcc tccgtggatc aattttgttt gaatgtgcat gtttctttcc
     2641 tcctataagt aaatgttttt tgataaatag cggtctgctg cgtctggaaa aatcgtaacg
     2701 acacgatgac caggtggtaa acgacgaatt tctcttaatg ctgctgcaaa agccgcacca
     2761 ctcgaacttc caactaatag tccttgttct cgtgctaact gcctagtata gttaaaccct
     2821 tctacatcag agattgtctc gatctgattg attgaaaggg gagaaagaaa cggcggaatg
     2881 aattctacac cgataccctc aatttcgtga ggggctggat caccaccatt taacacagag
     2941 ccctctggtt ctacaccgat tattcgaatg tcgggatatt tttctttcaa aaaggtagaa
     3001 gttccagcaa aggttccacc actcccaatt cctgctacaa aactatggat attttctttg
     3061 atttcatgaa agatttctgg ccctaacgta tgatagtaag cagcaggatt atctttattt
     3121 tcaaattgaa gaggcagata gctgtttgat atctcctctg caagtttttt acttttgctg
     3181 atggcaccga tgattccttg ctcactagga gaatggatga tctttgctcc caatgctttc
     3241 atcaattgtt gtttttctaa gctaaacttc tctggaacaa caaaaaccgt tcttaatttg
     3301 tatgtgagtg cggccagagc gatcccaatt cctgtatttc cggcagttgg ttcgatgatc
     3361 gtcgtttttt cattgatttt tccttgggca aatccttctt tgatcaaata actgcccaat
     3421 ctatctttga tacttcctcc aggatttaaa tattctagtt ttgcataaat agcagaacct
     3481 ttaggcatag aatagtgctg attgtcaaag cgataaagcg gtgtggaacc aatagcttct
     3541 gtaattgtcg agataatcat gtgggcctcc taaaaaaaga taataaaaaa aggagctgac
     3601 tattaaatca gctccttgaa agacatagag aaaaattgag tagataaaaa gataagttac
     3661 ctggtactca ttctatgaag ggcaattaat agacatgaca aatagtatgc tgcttttatg
     3721 caccatacaa cagcaacgat tcaattgttt ttggttgctg actgtcatgt gtatccctcc
     3781 aagtaaaatt tttattaacg atagcatgaa aaaaaataaa agtcaaaggc ttgaaagaaa
     3841 aaaatatttc gatagcatcg tttacccgtg tttatttttc tgacaaatag gcatgagacc
     3901 ttaaaagata gagaatactc tagttttctg gccggttgtc tgatacaata aagttgatta
     3961 tgataaagaa aagaaggagc aaaaataatg atctcagcaa gtgatttaag agccggtatg
     4021 actttcgtac aagacggcaa actgattaaa gttctggatg caagccacca taaacctggt
     4081 aaaggaaata cagttatgcg tatgaaactg aaagatgtcc gttcaggtgc aacatacgac
     4141 acaacgttca gaccagaaga aaaatttgaa aaagcacata tcgacacaaa acctgtacaa
     4201 tatttgtaca cgatggatga tacagctttc ttcatggatt tggaaacata tgagcaatat
     4261 gaaattcctg tagaaacagt agaagaagaa atgaaatata tcctagaaaa catggaagta
     4321 aaaatccaat ttttcggtag cgaagtcatc ggcgttcaat taccaacaac tgtcgtatta
     4381 cgtgttgtgg aaacacaacc ttccatcaaa ggtgcaacag tcactggttc aggtaagcca
     4441 gcgacaatgg aaactggctt agtagtcaat gttcctgact tcatcgaaac agatgaattg
     4501 ttggaaatca atacagcaga aggttcttat gtaaaacgtg cgtcaaaata attcgctaac
     4561 ggattgataa gaatttgaaa acagagcttg acctcttgaa aggcagatca tcggatctgc
     4621 ctttcaagag gttttttgtg gaatcagcaa attgaaaaat aaaaaagtat ttgattttat
     4681 ttatattttg aaagatgaat ggcaaaagct agagaaagaa gaaactgcac ttatttatga
     4741 agaaattgaa atgattcata aaattgaaaa tatccgaact attccgaact atattgaagc
     4801 gaaaatattt tttcatcact aaatagtgtt gaatttatgt ttttatgtat aaatagcttc
     4861 taatagttcg ggtattttgt ttttataacc atttttattc ttatttttaa aaaaatattt
     4921 cgaaatatac tagacaattc agaaaatttt gtttaatatt attccaataa aattcaaatc
     4981 tttatcaaat gaaattccaa taaaaacgga aaaaaagtga aaagaggaat gaaaaaatga
     5041 cagaattgat aattggcgga gtcacagcaa gcacattggc agaaaagtat aagacacctc
     5101 tgtatgtgta tgatgaagaa aagatgagac aaacgatgct gtcattcaag cgaggctttg
     5161 tatcgaaacg ttttgataca aaagtgcttt acgcctctaa agcattccaa acgattgaga
     5221 tgctgcattt agttcgtgaa tatggattcg ggctagatgt tgtcagtggt ggggaaatct
     5281 ataccgcttt gcaagctgat tttcctagag atactatcta cttccatggt aacaacaaga
     5341 cgccagagga actgatctat gcacttgaaa atgatttgtt gcatattgtt gccgataatt
     5401 tgatggaagt tgaattatta gcagaattaa gccaacgcta tttaaaaaaa atctgtatca
     5461 tgcttcggtt gaatgtcgga gtagaagcac atactcatga atatatcgtc actgcgcata
     5521 ttgattcaaa attcggcatg agctatgaaa gtaaagagtg ccaacaagcc cttcagttga
     5581 taaaagaaag cagctatcta gagttagaag gatttcatgc tcatatcggg tcacaaattt
     5641 ttgatgttac tgcttggtta gctgaaatcg acaaattgac gaattactta accgattttg
     5701 accaacctct ttccttgaat cttggcggag ggtttgggat tcgttataca caaaatgatc
     5761 agccgatccc agtcgaagag gcactaaaaa aactgattga atatacggaa gaagctttgg
     5821 aaaaacgaaa actgaccatt cgtcaattac tgatcgaacc gggtagaagt attgttggag
     5881 aagctggtac aacactatac acgattggtt tcatcaaaaa gacaccacat aaaaaatatt
     5941 actttgtaga tggcgggatg acagataata tccgtccagc tctgtatcag gcagaatacg
     6001 attgtgattt agcagcaaaa ctgtcggaag aaaaaacaga gaaagtgacg attgcaggaa
     6061 aaatgtgtga atcaggcgat gtggtaatta aagaagctta tttgccgaaa gcagaatcgg
     6121 gtgatctttt agctgtttat tccacgggag cttatggata ctcgatgagc agcaactaca
     6181 atcgagcgac acgaccagca gttgtttttg tcaataatgg ccaatcaaga ctgattgttc
     6241 gcagacaagc attttctgat ctactaaggg gggaagtttc ctatgatcct tcataaatat
     6301 aatggctgcg gaaatgattt tatcttatta gattacgcag aagacacaga ttatagtcaa
     6361 ttagccgctt ctttatgcac aaaagaaaaa tatgatacag acggattgat cgccgtaaaa
     6421 atcgatccgt tggaaatgat ctattacaac aaagatggca gccgcgcgcc gatgtgcgga
     6481 aatggcattc gctgttttgc tcgatacgta aatgatcaga acatgatgaa tgaacaggaa
     6541 ttcaacgtag agacattagc aggcacaaga aaaattacgg tgttggatcc ctcacctttt
     6601 tattgcagcg tagatatggg agtgccagat gattctccgg aaaaaatcaa agctaatcaa
     6661 acttatccaa taaaaaacca ggaaatagag atcaatggtg aaaaagtaac gatcactagt
     6721 ttatttttag gaacgattca tacagttgtt tttgttgaaa atgcgcgtga ggaagtctgg
     6781 agaaaaagag gtgaactgat ctgtcatcat cctttgttca aagaaaaaac caatgtcaac
     6841 tttgtagaag tagtcaatga acaagaattg atcgtccgta cctatgaaag aggagtaggt
     6901 tggacacttg cttgcggtac aggttgctgt gcctcttatg tggttgctaa ggaaactggg
     6961 aaaatttcag cagatcaggt agttgttcac ttggaacaag gagacttgat gatctctggt
     7021 gaggatgcga tacaaatggc tggcccagca gttcatgaat ggacaagaat attggaggaa
     7081 tgaagatgat aaaaggttcg atcgtggcgc tgatcacgcc gatgaatgaa gagggatcag
     7141 tagattatgc tggtttggaa aaattgatcc aattccattt agatgaacaa acagatggat
     7201 tattagtttt aggtacaaca ggagaatctt ctacgctcac acaatctgag gaggagcaga
     7261 tccttcaact gacagtaaag aaagtggctg gccgtgtgcc ggtgattgca ggagctggaa
     7321 cgaataatac gaaagaaacg attgaaaaag cgaagcattt tgcttctttg ggggcagacg
     7381 cacttttggt catcacccct tactacaaca aaacaagtga tgcaggacta gctgctcatt
     7441 ttacagcaat tgcagaagcc tcaccgattc cgcttatttt gtataatgtt ccttctagaa
     7501 caggcatgtc gattccaatc catgtattag taaatttagc agaacatcct aatatcattg
     7561 gtttgaaaga agcctcagga gatatggctt atgtgatgga tgccgctcgg ttgattggag
     7621 aagaattctt cctttacagt ggaaatgatg atttgatcct accagtaatg agtgtaggag
     7681 gaagcggtgt aataagtgta tgggcgaata tccaacctaa aatagtccat gaattagtaa
     7741 aagatacgca ggacggaaga tggcagcaag caaaagaaaa gcaattaaat gcacttgaac
     7801 tgatccatgc cttgttcagt gagaccaatc ctattcctgt caaagcagcc atgtcattat
     7861 tagacttacc ttccggacct ctacgcctgc ctttagtttc tctttcaaaa gagaaaaaga
     7921 aacaattagc tcagcttttg ttaaaagaaa ggacggttga aaaatgaaaa tcggactgat
     7981 tggcagtgga cagatgggaa atagagtcga agaagttgca caagaaagag gagatacggt
     8041 ccttctttta cggacggcac ctaaagaagc aactacgctt gcttttacgg aactgccaga
     8101 actgcttatt gatttttctc atccagataa tttagagaaa atcctttcgt atagcttatc
     8161 atatcaactt ccgttagtca ttggcacgac aggttataca caatcacagt tccaagaaat
     8221 aaaagaacat gccaaacatg tacctgtgat gtatagtgct aatttttcgt tcggcatcat
     8281 ggtgatgaat caactaatca agcaagcagc tgcaatgttg caaggctggc agatcgaact
     8341 gattgaaaaa catcatagtc gtaaaaaaga tgcaccatct ggtactgcaa atatgctgct
     8401 tcaaacaatc caagaagtgc aaaagctgca gcccgtatac aactggcaag ggaaaaaaag
     8461 agaagaaaac caaattggtg tacacagtat tcgtgcaggt tctttacctg gtgaacatga
     8521 tgtattgttt gctgctactg atgaaatatt tacgatcaaa catgaatctt tttctaatcg
     8581 aatctttgct gcaggcgcag tagaagcagc ggactggttg aaagatcagt caccaggtta
     8641 ttacaaatta gaagatttat taatggacaa aggggtgtaa aaaacgtgga agctcaagaa
     8701 atcatccgct acattcaaac ggcatctaaa aagacgcctg tgaaagtcta tctgaatgta
     8761 accgagccaa tcgaatttaa agaaagtaaa gtttttggtg aaggaagcag cttcgttgtt
     8821 tttggtgact atgagacgat tgctccggtt ttagaagctg aatcagaaaa aatcattgaa
     8881 atcgaaatcg aaactgctgc aagatacagc gcagttcctt tgctagatat taaaaaaatt
     8941 aatgctcgta tcgaaccagg tgctattatc agagatcaag tgtcaatcgg caacaatgcc
     9001 gtgatcatga tgggagcgat tatcaatatt ggtgcagtaa tcggtgaaaa cacgatgatt
     9061 gatatgggtg ctgtcttagg tggacgagcg acagtgggca aaaactgcca tattggtgca
     9121 ggagctgtct tagcaggtgt gatcgaacca gcatcggcca aaccggttat cgtagaagac
     9181 ggcgtactgg taggtgcgaa tgcagtgatt gttgaaggtg tccatatcgg aaaagatgca
     9241 gtcgtggcag caggagcagt ggttttagaa gatgtggcag cagaaactgt tgtcggtgga
     9301 atacctgcac gtgtattaaa aataagtgat gatcagacaa aagaaaatac agcattgatt
     9361 gctgcgttac gcgaattata gaggagatgg agaaaaatgc gcgaaataga tacctgtatt
     9421 gtgggggcaa ccggattagt cggccaaacg atgctcaaag tgttagaaga atatgatttt
     9481 ccagtaggac gattgaaatt attagcttca aaacgttcag ccggtaaagt ttgtgtattc
     9541 aagaaaaaga aacacactgt acaggaattg acagaaactt catttgaagg atatgattta
     9601 gcgctatttt ctgcaggtgc ttctgtatcc aaagagtttg cgccaatcgc acgaagtcag
     9661 ggactatttg tcatagataa ttcctcagca tggcgggaag attcggatat tccattaatc
     9721 gttccagagg tcaatctgga agactataca ttgaatcggc tgattgcaaa tccaaattgt
     9781 tcaaccattc aagcggtact tccgcttaaa gctttacaag aagcctttgg tatccagcga
     9841 gtcaattatt cgacctatca agcagtttct ggttccgggc aaaaaggcat tgaagatctt
     9901 catcgtacgc aaaatgacga ggaaccagtt ttgtatcctt ataatattag ccaaacggtg
     9961 attccggaaa tcgatcagcc tttggaaaat gggtatacaa aagaagaaca gaaaatgata
    10021 aatgagaccc gtaagattct gcatcagcct gctttgccag tatcagcaac ctgcgtacgt
    10081 gtgccaatcg aaaatggaca tggagtctct gtggcagttc aactgcaaaa agagtttacg
    10141 acggaagaaa ttagagagtg tctcagccag tttccaggga ttcaattaga agatgatcta
    10201 gcagcacata aatatccgac gagcattctt gctagaggaa cagatatggt ttatgttggg
    10261 aggatcagaa aagacacttc tttagagaac ggattgttgt tctatacgac tgccgataac
    10321 atacgcaaag gagccgctgc gaatgccgta cagattgctg cagctttaat gcagaaaata
    10381 gcggaggtgg caagatgagt aatacgctga aagtagcgaa attcggcgga acatcgatgt
    10441 ctcatgaaga gcaattcaaa aaagtccgta caattgttct ttcagatccc aatcgccaag
    10501 tgatcgttgt ttctgcttta ggcaaacgta cgtcagatga tgacaaagta acagacttac
    10561 tttatttgat ttatgcttac cttcagcatc atgttgattg gacacccctt tggaaaagga
    10621 tttgtgatcg tttcattcaa gtaagagatg ttcttgccct gcagttacct attgaagatg
    10681 aactagcacg catacagaaa caactggaag acgaaaagat aactgaagat tatttagtaa
    10741 gtagaggtga atatctgact gctcaactga tggctgagta tcttggttac cagtttattg
    10801 acgcaaaaga cctgattttc tttgactatg atgggaaaat cgatcaggaa aaaacagcac
    10861 aggaaatcac aaaagcattt aaaagatatc cgaaaatggt tgttccaggg ttttacgggg
    10921 cgaaccctgc aggaaaagaa aaattattgg gccgtggggg aagcgacatt accggcgcga
    10981 tactggcgaa gatgttgcaa gcggaaagtt atgagaattg gacggatgtt tcaggaatca
    11041 tgatggctga tccgcgaatc atcgatcatc ctaaaatcat cgaggaaatc tcttttagag
    11101 aactgagaga gatggcttat atgggggcta acgttcttca tgaagcagct gttttcccag
    11161 ttcaagaggc tgatatcccc atccaaataa aaaataccaa tgatcctttc gctcaaggga
    11221 caaaaatatc gaatcacgaa atagaaaagg aaaatggact gacaggaata gctggacgca
    11281 aggattttct ttccatcact ttattcaaac gtcatatgtc cgatgaaatc gggtttatct
    11341 ggaaagcaat gagcattttt gctaaacatg agatatcaat cgaacatatt ccttcaggga
    11401 tcgacaatat cggagtgatc gtctcagcag aagcgattgc cgataagtta tttctcatca
    11461 cgaaagaatt aaaagaaaca cttggcgtgg aagaaatcgt cgtgatcgaa gacttagctt
    11521 tgatctctgt cgttgggggt ccgcataaag aattgattgg attatctggg aaagtgttat
    11581 cgatattgaa caaattagaa attcgcacat cgatcttatc acaaggagca caggagttga
    11641 atctgatcat cggtgtaccg aataatcaat atgaaacagt agtgaagggt atctatgaag
    11701 ggatggtgaa tacggatgct gaaagaatat cgaatggcac tccatcaaat tcctgaaatc
    11761 gggtttcatg aattcaagac aaaaaaatat ttatatgaac aagtaaaaaa cagcggggcc
    11821 gttatccatg aaatcggcga aacaggacta ctcctttatt ttgataatca gcaagaagaa
    11881 acgattgctt ttcgaacaga tatcgatgca ctgccagtca aagaagaaac gaatctctct
    11941 tttgcttcta agcatgaagg ttatatgcat gcatgtggtc atgacggaca tatggcgatg
    12001 cttttagggt tgactgactt tataactaaa cacattgagg atcttattta taatgtagta
    12061 ctgatctatc agccatcaga agaaatttcc ggtggcgcac aaagcgttat tcagtcagga
    12121 ttgttggata aatatgaagt acaagcaata tttggctttc atctatggcc tggactagct
    12181 aagggagaag ttttctctcg accaggacct ttgatggcac aaagcagtga aacagacatt
    12241 atcattaaag gccagtctgc acatattgct tctagtgaaa aaggaatcga tagtctggag
    12301 gcagctgtac gatttttgaa gaatgtttat gattttgacc ggcaattacc ggcagagtgg
    12361 aatcatctaa tgaaatttgg ccaaatccaa ggtggaacga tccgcaatgt tttagctgga
    12421 gaagttattg tatccggaag tatccgttct tacagttcag atatgcaaaa ttatttgaag
    12481 aggggattag accatttagc agaacagttt cactctacag ctactatttc ttttcgctat
    12541 aatgacggct atccagctgt aatcaaccat agaggcttat atgcagcact gttagattca
    12601 aacaaactac atgaattaga tgctccagtt cttcaagccg aagattttgg catatatacg
    12661 caacaatatc catgtgtatt tttcttttta ggtgtcggtg atacccctgc tttacatgat
    12721 ccgacgttcg attttgatat ggatgtttta gaaaatggtg tagaatggta caaaacgatt
    12781 ctttttacag aaaatcttct tgtaaaaatc gatagcagtt caaaagagga gcgagtaaat
    12841 aatgatgaat ttatccaatc gatttagtcc taggctttct agaatagaag tatcaaaaat
    12901 acgacaattc gatcagcaaa tatcgtcgat accagatgtg atcaaactca ccctgggaga
    12961 accggatttt ccaactcctg aacatgtcaa gcaagcagga atagcagcga ttgaagaaga
    13021 cttttctcac tataccggaa tgagaggatt agaggaactg cgggaagcgg cttgtatatt
    13081 tcagcaacag cgttatggat taacttatga tccgcaaacg gaagttttga cgacagttgg
    13141 tgcaacggaa gcgattgcct ctgcgttgtt atctgttttg gaagaaggag acaaggttct
    13201 tattccagcg cctgcttatt caggatatca gccattagtg gaactagctg gtgctgaatt
    13261 gatcccgata gatacttctg acacagggtt tgtctgtcag ccggaacaat ttgaacgagc
    13321 atttgaacaa tatggatcag ctgtcaaagc agtcatttta aactatccta acaatcctac
    13381 tgggacgaca ttgtctgcaa aacaattgaa agcaatcgct gaggttttaa aaaaatatcc
    13441 ggtatttgtt ataagtgatg aagtatatgc tgaattgact tatagtggaa cgcatatgtc
    13501 catagctact tatcttccag aacaaacaat cgtgataagc gggttatcca agtcacatgc
    13561 gatgactggt tggcgagtgg gctttatttt tgctcaaaaa gcccttattg acgaactaat
    13621 taaagtccat caatatctgg tgactgccgc tacaacgatg tcgcaaaaag ctgcggctga
    13681 agcgctaata aatggggtga aagatagcga gaatatgaaa gagcaatatc aaacacgaag
    13741 agactatctg gtccagcaat tggcgccttt aggatttgaa gtgacccagc caaacggtgc
    13801 attttacttg ttttgcaaac tcccagctgc cattcaaact gactcttggc agttttgtgt
    13861 agaattagcg gaacaggcaa aagttgcgtg tatcccagga attgcatttg gcccagaagg
    13921 agaaggttat atccggattt cttatgctag cagcatggaa aaattacatg aggcctgcaa
    13981 acggatcaaa gactttttaa cagaacagtg attcatagat tttatcaaaa aaacaatcgt
    14041 taattttgcg gtatattctg caaggagacg attgtttttt aaatttagtt caaatgaacg
    14101 cgtttctttt gattcataac taatgtaatg ttaagtttaa attgtttaga atgattttaa
    14161 aagaatattc aaggaggttt catcaatgga aaaaatcaaa gttgtcattg taggtgcatc
    14221 tcatggaggg catcaatcga tcttggaact attgacacga tacggtgaaa atgtagatat
    14281 cacgctattt gaagcaggag actatatttc cttcatgtca tgtggtatgg agctgtattt
    14341 agaagacaag gtaacagatg taaatgatgt acgtaatttc agaccagaaa actttccgca
    14401 atccaatgtt catattttaa ataatcacga agtaaaaacg atcgatgccg ataaaaaaac
    14461 agtaacggtt actagcgcat cagataacga aacaaaagaa tatccttatg acaaattgat
    14521 tttgagttct ggtgtcaaac caaacaatct gccagtacca ggagcagatc tagaaaatgt
    14581 ctacttgatg cgtggataca attgggcaac caaaatcaaa gaaaaattaa atgatccggc
    14641 tattaaaaaa atcgctgtcg taggtgcggg ttacattggg atcgaagcag cagaagcaag
    14701 ccgaaaagct gggaaagaag tcgtattgct tgatgtgatc gatagacctt taggcactta
    14761 tttagatgct gagatgacag atattttaga acaacaccta aatgaaaacg gtattgaagt
    14821 tttgacaaat gcaaaaatcg aagcttttac tggtaacgga aaagttgagg caatcaaaac
    14881 aagtgaaaaa gaaatcccag cagatctagt cattcaagct gccggtgtga aggcaaatac
    14941 agaatggtta aaagggatcg tcgacttaga tgaacgtggc tggatccaaa caaatgaata
    15001 cttgcaaacg aatcttccag atgtgtatgc agtaggagat gctacacttg cttattctat
    15061 cccagcacgc aaaaaaatgc cgatcgcttt agcgactgtt gcacgtagag aagcacgtta
    15121 tgtagtaaaa catctttttg aagaagtacc aaacaaaccg ttcggcggag tcgtaggttc
    15181 ctctgcatta agtgtctttg actatcattt tgcagcaagc ggattgaata gctttacagc
    15241 agcaaaagca ggagtagatg tacgagtgtc ttattatgaa gatacgttac gaccaaaata
    15301 tgtaccagaa gaacaaggaa atccaaaagt gtccatccaa ttattctcgg atccaatgac
    15361 ccatcaattg ctaggaggag cagtattatc tacctatgat gttacaggtc aaggaaatgt
    15421 attggcacta gcgatccaaa acaaactgac tttagaagaa ctagcagaag ctgacttctt
    15481 cttccagcca ggctttgacc gacaatggag catgttgaat ctagccgctc aacaagcgtt
    15541 aggagaagaa ccatttgtag aataagaaaa cgaaatattt gaaaagaggt tgtgaaaaag
    15601 ctgccttgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg tttttaactt
    15661 ggctggactt attaccgcag atgcaagctt ttgaacaccg tttattttca aaaaagggac
    15721 tgtgacacga tttgtgtacc gacccccaaa agttagacca aaaaatctaa cttttggagg
    15781 tcggtatttt ttatggttaa atatactttt gaacttaaat taaagatcgt tcatgattat
    15841 ttggatggca aaggcgattg gaccgtaaaa atccagatca agagattgaa gcggaaatgc
    15901 ttaaaattcg ggagaagcac aaggattatg gctgtttacg gatgaccaat gaactaagaa
    15961 accgcggttt ctctatcaat aaaaagaaag ttcagcgctt gatcaagaag ctaggaataa
    16021 aagtaacggc atacacgcgt aaatcacgtc gctacaattc ttatcgagga aaagttggag
    16081 cagtagccaa aaatcggatc catcgtcgtt tctacaccag tatctgccat cagaaaatca
    16141 cgacggatac aactgaattt aaataccatg aagtggatgc caaaggcatt atccgtcaaa
    16201 agaaattgta tctagatcct tttatggata tgttcaatag tgagatttta tcctatcgta
    16261 tctctgaaaa gcccaatgca ttagcggtaa tggaaggttt ggaggaagct atccagacca
    16321 ctaacgattg tccctatcgt cgtacgtttc actcagatca aggttgggcg tatcaaatga
    16381 aagcttatag aaacaaattg aaggagtaca aaatctttca aagcatgtct cggaaaggga
    16441 actgcttaga taattcactt atggagaatt tttttggctt attaaaacaa gagatttttc
    16501 acgggaaagt ttataactgt ttcgaggaat taaaatcagc catcgatagc tatatctatt
    16561 actacaataa tgaacggata aaac
//
LOCUS       DACDYJ010000060        16540 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDYJ010000060 DACDYJ010000000
VERSION     DACDYJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.060"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..338
                     /locus_tag="IWM51_002026"
     CDS             <1..338
                     /locus_tag="IWM51_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9971434.1"
                     /translation="DSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHAS
                     VSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRIT
                     FMSYKKSKR"
     gene            complement(493..1479)
                     /locus_tag="IWM51_002027"
     CDS             complement(493..1479)
                     /locus_tag="IWM51_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9971435.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1502..2068)
                     /locus_tag="IWM51_002028"
     CDS             complement(1502..2068)
                     /locus_tag="IWM51_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP9971436.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2082..2498)
                     /locus_tag="IWM51_002029"
     CDS             complement(2082..2498)
                     /locus_tag="IWM51_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP9971437.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(2955..4889)
                     /locus_tag="IWM51_002030"
     CDS             complement(2955..4889)
                     /locus_tag="IWM51_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9971438.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5191..6126
                     /locus_tag="IWM51_002031"
     CDS             5191..6126
                     /locus_tag="IWM51_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP9971439.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6216..7319
                     /locus_tag="IWM51_002032"
     CDS             6216..7319
                     /locus_tag="IWM51_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9971440.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7332..8285
                     /gene="menA"
                     /locus_tag="IWM51_002033"
     CDS             7332..8285
                     /gene="menA"
                     /locus_tag="IWM51_002033"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9971441.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9099..10445)
                     /gene="gor"
                     /locus_tag="IWM51_002034"
     CDS             complement(9099..10445)
                     /gene="gor"
                     /locus_tag="IWM51_002034"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP9971442.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10579..11820)
                     /locus_tag="IWM51_002035"
     CDS             complement(10579..11820)
                     /locus_tag="IWM51_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9971443.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(11838..12761)
                     /locus_tag="IWM51_002036"
     CDS             complement(11838..12761)
                     /locus_tag="IWM51_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9971444.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(12982..15474)
                     /locus_tag="IWM51_002037"
     CDS             complement(12982..15474)
                     /locus_tag="IWM51_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9971445.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15481..15948)
                     /locus_tag="IWM51_002038"
     CDS             complement(15481..15948)
                     /locus_tag="IWM51_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP9971446.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(16084..16157)
                     /locus_tag="IWM51_002039"
     tRNA            complement(16084..16157)
                     /locus_tag="IWM51_002039"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16121..16123),aa:Arg,seq:acg)
     gene            complement(16215..16288)
                     /locus_tag="IWM51_002040"
     tRNA            complement(16215..16288)
                     /locus_tag="IWM51_002040"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16252..16254),aa:Pro,seq:tgg)
     gene            complement(16310..16383)
                     /locus_tag="IWM51_002041"
     tRNA            complement(16310..16383)
                     /locus_tag="IWM51_002041"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16347..16349),aa:Arg,seq:acg)
     gene            complement(16394..16479)
                     /locus_tag="IWM51_002042"
     tRNA            complement(16394..16479)
                     /locus_tag="IWM51_002042"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16443..16445),aa:Leu,seq:taa)
BASE COUNT         4858 a         3475 c         2963 g         5244 t
ORIGIN      
        1 gtgatagcac ggacacgtca gcttcaagtg acagtacaga tacatcctct accacaagtg
       61 atagctcaga ttcatcaact tcaagcgata gtacagacac gtcgtctacc acaagcgata
      121 cacgtcatgc aagtgtctcc ttctcaaaaa caagttcgga taacaggacg aactttaact
      181 taactaataa aacaagcaat gacaatggaa acaaatcaaa aagagcactt cctaaaactg
      241 gaagtcaatc gaataattgg ataacactcg caggcgttat attacttgta atcggtttaa
      301 gaatcacttt tatgagttat aagaaatcga aaaggtaaaa aaatattcaa gataaaaaag
      361 agcaacgtcg ctaacggacg ttgctcttta tttttgcttt ttatcataaa tacaagaact
      421 acaattcaat ttgtcaaaaa acacgtagat ttccttctga gaaatttatt tacgtatcga
      481 acctttccct attcagtctt tgcgatccaa aatcagttgg gtaagggaaa agagattctc
      541 ttttgttcga agagaggtgg caggtaattt ctttatttcc ttcaaagcct tttctgtgta
      601 agaggccgct aacttcctag cttcttccac gcctcctgta tgttcaacga ttttgcgtac
      661 ggcgtcgaca tcttcttctg tcattgccgc tttcttctct aaataaggta gtagctttgg
      721 tttgtttttt tgcaaagagt agatcaacgg taaagaatag accccttgac gcatgtcttc
      781 tagtacaggt ttgccgattt cctggctctc ttttgtataa tctaaaatat catcgataat
      841 ctgaaaggcc atcccgatag aaagtccgat atctcctgct ttttttgcga agcgctcgct
      901 tgttccactt tcaaaagcgc caacagaaca acttaattgg aacagttcag ctgtcttacc
      961 tgaaatattt tccaaatatt cttttacaga aagttcataa ttatagcgat catccatctg
     1021 ccctaattcg ccattcaata ctttttccat actgcgagaa ttcaattgga gacttttcaa
     1081 ggaagtggag taatcagaca atagtttaaa acagcagaca aataaatagt ctcctgcata
     1141 gacagaaacg acattaccaa actgactacg gatattaggc tgaccacgtc gcagatccgc
     1201 ttcatcaacg atatcatcat ggatcagtgt ggccgtatgg agcatttcga tagaagctgc
     1261 caaagcgatg gctttgtctc gatcttgctc tggtccaaat tgtgagaaca atagctgata
     1321 ggcaggacgc aacagctttc cgccggcatg gatcgtttct ttgatggctt gttcgacagg
     1381 tttgtttttc aggcggacag tttcttccat ccgttttagt gtttctgtca attctttact
     1441 taaagcagga tagttttccc acattggatg tagcttcata aaaaaactcc ttgtgtgacg
     1501 attatttttt ggtaaacatt gggctccatt tttgtttggt tccctgctca taatcttttt
     1561 ggaatcgttg aagtgtctgc acatgtcgta aaagataatt cgctgcgatc ccgattgcaa
     1621 tccctgataa aatccctaaa actgaaagga ctggcaagta caacatgact gtccatgatt
     1681 gtgcaatgaa agaagccatc aaaagctgtc cgacattatg catgaaaccg ccggccgcac
     1741 taattccgat gatactgaca cgttttggtc ctaattgctt gaccaataac attccaaaat
     1801 aactaagcaa agcaccactc atactataaa ggaatgtgga aatcgttccg cctagcaaag
     1861 tagtcaggaa tagtcgtagc caaagtagta agaagctgtc ttgttttttc atggtaaaaa
     1921 tggcaacgat cgtgattaga ttggctaagc ctagttttgc accaggcgca aaagaaaaag
     1981 gataagggat catattttcc aataaaccaa tgatcactcc ttgtgcaaca agtagcgaaa
     2041 tataaatcat tttttgtaat ttactcatgg tgtgtgaagg tctaataaat cagactgtct
     2101 tcgtcacctc catctgttga acggacttcg atcactaatt tatgtggcaa acagacgatg
     2161 gtttcgccat ttttcgttgc ccagcctcga cgtacgcaga tctggtcatc gcaatctgct
     2221 tcttttatcc gtactcgatc gccatctatc tcgatcaagt tataatctcc gtgttcatct
     2281 ttgtaagtat atgtgttgct ctcttctcca gccatcaaag ggaatgtttt gatttctgtg
     2341 ccatccacac gcagtaccgc ttctttttct gctgttgcgg tgttttgctg aagaccaaaa
     2401 atcatgactg gtaaaaaaga aagaacagtc aaaagcaaaa taataaaaac atcccaaggt
     2461 ttgagcagac ttttttcagc aaattcttta aggctcattt ttgttctcct ttttccagat
     2521 taactcaccc aattttagca taaaaacaag aggttgtgac acctgtgtct tgctttgagt
     2581 aataagaacg aagaatagaa aacagctttc catgttttct aattctttgg gcttattacc
     2641 gagaagcagc aggtggaaca ccgtttataa caagaggttg tgacagaagt gggcaacttc
     2701 aagaaataag aaggaattta cgaaaattac ttttcaagtt tttgtgaaat ttcggcttat
     2761 ttttcgaaga agctacttct ggaacaccgt ttataacaag aggttgtaac agaggttgtg
     2821 acacctgtgt cttgctcatg gaggcttatc tcgggcatct tattccttta attttacata
     2881 aaaaggaggc tgtgacggtt tgtctcagcc cctttctatt gaaatcttgc attcaaataa
     2941 cgttctttat ctatttattt cttgccgtac tgggaacttg gtctaccgta ccaccaatta
     3001 cctagtgtac gttgtagcca tggaattacc cagcgatcca acccaatcgc acgtccggaa
     3061 ccgttcatca atgcgaaagc aacaaaaatg aaccaaatgt ttacccagta aaacatgcct
     3121 gataagcaga acgtgatcac caatacaatc gttgtcgcac tgcatagcca tgtgaataat
     3181 ccagcaatca atgctaatgc caaggcaatt tcaaagaata ccatgaattt ttggaagaac
     3241 attgctacat cattgtttgg catgatcact tgcatgatct ttgtgaacca gtctggtgac
     3301 ttctcaatca cagccattgg ttcttcaccg tatgcataac tcaaaccaaa atgtgcagct
     3361 tgagtcgctg cttctccggc acttgcccct gctccgctgg cagctgtggt tgcatcacca
     3421 gcagcagaag caccactggc aacatctgtt acattgtatt cccatgtcca agggaacaca
     3481 ctgtttccag tgaaccatga tccttcacca aaccaagtac ttggtttgaa cacttcacca
     3541 ttcccaatga tttttttgga agcttcaacg aaccaaacta aaccatagaa aatacgaagt
     3601 ggcacactcc ataagacatt gccgtaacga gaagaatgtc ctcgagtcac gttgcgatca
     3661 tctttgatat ggaaaaactc atgcattaaa tattggaaca tataataacc agaacgaata
     3721 tcaaagaagt atttcaaatt cacgatatgt ttcattacga tagctaaaaa tccgcttaaa
     3781 tggatcttat caaataggtt agctaccccc catttggcac caatagaaac cataaatcct
     3841 tgatagtttc ctttaaattt atgcttttct ccgcctttga tcgaagccac gatattcgct
     3901 gcggctgtgt gtcctgtttg ttcagcagct tgaacgatct gcggtgtcgg tgttcctggg
     3961 aattcttcat aaaagactaa atcaccaatg atatagatgt ttttatcttc gtagcctttt
     4021 gcctgcatat attcattggc gatcaaccgc tgtccgcgtg ctgattctaa tccaaagtca
     4081 gcagcgtcag aagttccttt gacaccagct gtccaaatca gtgtgtgagt cggaatgttg
     4141 cgtccgtctt tcaatttgat atgatcagca gccacttcta cgattggtgc attcaataat
     4201 aattccacgt tgtgcttacg caaatagcgt tcagcttttg cagcatcatt tcgatccaac
     4261 atattcaaga tcgtcggcat tgcctctacg accatcagtg tgatttcgtt tggatctaat
     4321 ttataatctt tggctaggcg atttttccag tcgatcagtt ccccaatcat ttcaataccg
     4381 gtaaatccag aaccacagac aacgaaagtc agcatggctt tacgcagttc atcatctggt
     4441 tccaatgcag cttttgctac tgtcttctca atatgttccc gaatccttac agaatcttca
     4501 aatgaccaaa gggtgaatcc gtgtgctttg acaccaggtg taccaaagtc attcggctct
     4561 ccgcccattc caaggatcag ctgatcaaaa ggataggaac ctaatttggt cttgacgatt
     4621 tttcgttctt tatcgatacc gattacagtg tctgtgacaa gttgtacatt cttttgacga
     4681 gcaaacaaat gctgcagatc gtattggatt gcttctggtt ctacacgtcc gccagctact
     4741 tcatgcaact cggtcatcat agtatggtac gaatgacgat cgatcagagt aattgctaca
     4801 tcttcttttt tcagtttctt tgccaagtat tttgtagctg aaacaccggc atatcctgct
     4861 cctacaacga caatattttg ctttgccatt gatcatccgc tccttataga ctatctatat
     4921 aatgaatata tgataattct tttagtttga tacttattac acaatctcca tatattatat
     4981 agatgttctg ttcttttgtc taactccttt tggatttttt tcaaatttta aaataaagcg
     5041 ttttttcacg aaatttgctg aaagaaaacg tttgaaatat tacatgggtt atttatcaaa
     5101 aatagttgac ttataggcta aaaagattag aatgtataat gaatcacaaa gaaaaattgt
     5161 ggactagaaa tggaatggtg gggtattaaa atgaagaaaa aacgttttgt aactggattt
     5221 gccgtattag cattttcagc tctagtgtta ggcgcttgcg gcgcagatga caacggaagc
     5281 tcaaactctt cttctgaatc atctacagca caatcttcga ctgcaaaaag cacaacagaa
     5341 tcatctgcaa aagttgtagc tggcggagat ttgcaagacg gcacatataa attagaagaa
     5401 aaaaattatt caaatggtta ccgtgcagtc tttgaaatgg ttgtaaaaga cggcaaaatc
     5461 actgaatcaa aatatgataa cgtcaatgaa aacggcgaat caaaaacaaa agatgcagaa
     5521 tacaacaaaa acatggaagc aaaatctggc acaaacccag aaaaatacat tcctgagttg
     5581 aatgaccaat tcttaaaagc acaaagcgca agcggtgtgg aagtcgtaac tggagctact
     5641 cactcatctg aaagcttcca aaactacgca caacaattga tccaagctgc tcaagctggt
     5701 aacacagata caatcgaaat cgacaatggt gctgatttga aagacggcac ttacaagctt
     5761 gaagaaaaga actactctaa cggctaccgt gtgcaattcg aaatgacagt tgctggcggc
     5821 aaagtaactg aatcaaactt tgattatatc gacaaagacg gtaaatcaaa acaagatgat
     5881 acagaatata atgaaaatat gaaagcaaaa agcggtacag aaccaaaaac ttacatccct
     5941 actctgaacg acgaattcgt caaagcaatg ggcgaagaag atggctcacc tgctaatgta
     6001 gaagttgtta caggtgctac acacagctca cattcattca tcatgtacgc acaacaatta
     6061 gtgaatgcag ctgaaaaagg cgacactcaa acaatcgaag tagataatat cgtaacagaa
     6121 aaataatcac ataatcatac taataaaagt tggagggtgg gacgatagcc agattaggca
     6181 gtcgtctcac ctttcaaaat gaagagagga aaaagatgcg gaaaaaacta ctattttcct
     6241 taatcacact gggcatctct tttatggtgc taactggctg cagcaactca gcaaataaaa
     6301 caaccactga aagttcagca gctaccaaga tattgaaaga tccatattct gatgaacagt
     6361 tcttgttagg gacttacgtt cgtatccgtg tgtatgatga aggcaaagaa tcagcattga
     6421 aacctgcctt cgatcgagta aaagaacttg gtgataaaat cacgatcaat caaaaaggtt
     6481 ctgagatcga tgaagtgaat gagcaagctg ggatcaaacc agtaaaagtt tctgatgacg
     6541 tctacacgtt agtcaagcgg gcctacgaat atagccaaga ttctcaaggc ggattcgata
     6601 tggcgattgg tgcaatcaca caattatggc gaatcggttt tgatgacgcc agaaaacctt
     6661 cccaagaaga aatcgatcag gcattgaaat tagtggatta tcataagatc gaactaaatg
     6721 acaaagaaaa aactgtttat ctaaaagaaa aaggaatgat catcgattta ggtgcaatcg
     6781 ccaaaggtta cattacggac gaagtagtga aagtcttgaa aaaacaagga gtgaccactg
     6841 ccatcgttga cttgggcggt aatgtctatg tcttaggcca cagcccaaga ggcgaaaacc
     6901 aagactggac agtcggcatc caagatccga acatggctcg cggatctgtt ttaggttcta
     6961 ttaaagaacg gaacaaaaca ttagttacct ctggaattta cgagcgttat ttagaagtag
     7021 atggcaaaaa ataccatcat ttgtttgatc caaaaacggg ttatccgttc gacaatgata
     7081 tcgcaagtgt cacgatcata acagataaat caattgatgg tgacgggttg tctacagcag
     7141 tcttttcaat gggtgtgaaa aaaggactgg aatacgtcga aagcgaattg aataacggga
     7201 cggaagcgat cttcgtgaca aaagacgata aagtatatgt gaccgatcca atcaaagaca
     7261 ccttcaaatt atcggaggac tcccattaca cgatgggtga ccgttctgaa ctaaaataag
     7321 gagtacgaac catgacacta aaaatctttt tagaagtagt tgaaatacga acaaaggtgg
     7381 ctagtgtatt tccttttgcg gtaggggtat tattctctat cgcttttttt catgagatac
     7441 attggctaaa cactttttta ttttttattg gaatgctagt ttttgattta gcgactaccg
     7501 ctatcaataa ttacatggat tatgaaaagg ctcattctga agtatataag tatgaagaaa
     7561 atgtcattgg ccgcagaaat ctatctcctg tattgattcg aaacatgatt ttagctatga
     7621 ttgcatttgt ttttgttatc ggcgttattc taacgatcaa tacgggttgg cttttacttt
     7681 tgatgggatt tatctgttgt tttattggag tattctatac attcggaccg attcctttat
     7741 ctcgaatgcc tttaggggaa attttcagtg gggtgactat gggccttgga atttttgcca
     7801 tggtcgttta tgtcaataca ttcggttcag atgtctatga tctgacagtc aacttttcat
     7861 ctggacaatt tgatttggta ggaaatcttt gggctatagc agcaatcgta ttagcttcct
     7921 tacccttggt gtttacgatt gccaatatca tgttggctaa taatttgcgg gatcttgacc
     7981 gtgatattga aaatcatcgc tatacactag ttttttatat cggacgtcct gcgggaacag
     8041 tcttattcca gctattgatg tatgcttgct atttcgtgat tttaatgggg ttgttgagcg
     8101 gtatctatga atggccgatc ttggtcacct tcttgacctt gccaattgtg aagaagcatt
     8161 tggatcaatt caagcaaacg ttaccccagc ctatcagttt cagctattcc atcaaaaata
     8221 tggtcttatt caatgccagt tatgtattgg ggctattact aacaatcatt atgcaagcca
     8281 tttaacagca tgaagaggtt gtgaaaaaac tgccctgcat caagtaataa gaacagccaa
     8341 ataaaaatag ctccttatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
     8401 tgaacaccgt ttatcaggag gttgtgaaaa agtcgcttag tttcgagtat taagaaaaat
     8461 tttgtgaaaa tggctttcca catttttgca taaatttggc ttaataccga gaaactggct
     8521 tttgaacacc gtttatcaga ggttgtaaaa tcagtgtttt gtcctgagta ttagagaaac
     8581 aaatggaaaa atagttcctc atattttgcc gttttttgag ctaataccga aggacagctg
     8641 attgaacact atttgttcgg aaaaagtgac taactatgac acaatttttt tcacagtccc
     8701 ttttttcact tgatttgatt cgtaccagcg aacttgacga gaactttctt ggtttggtaa
     8761 ctatatggta ttcttttaac atcctcgttt gcttcttcta atttatttat acaaagtcac
     8821 acttttttaa acatctgttc ttcgatacat ttctatttct tttaaacatc gataccaata
     8881 ttttgaactt aagagtgcat accaaatgga ttcatttact cttcgtcagt tacgaagata
     8941 atcaaaatct ccaacttccc tacttatttc gttttgagaa ttatcgaagc aatcttacat
     9001 aaataactaa aaaacaaaaa accagaaaat cagtatactg attttctggt tttacatggt
     9061 ttgttcaata aaaagctgtt ggaactgtta atattaaatt atcgcatcgt tacgaattct
     9121 tccgctcctg ttggatggat ggctactgta ttgtcaaagt cttctttagt tgcacccatc
     9181 ttgatagcca cggcaaaacc ttgcagcatc tcatcgacac caacaccaat agcatgtaag
     9241 ccgacgattc tttcttcttt tccgacacag atcaatttca tttcgcattt ttggcgataa
     9301 tcatttaatg caaagtacat tggggtaaag cgtgaacgat aaactttcaa ttcattttcg
     9361 ccatattttt ccaatgcttc ttcttctgtc aaaccaattg ttgcaattgg tggatgcgta
     9421 aagacgactg tcggaacaag tttatagtca agataagaat ctttctttcc attaaataaa
     9481 cgttctgata aacgtctacc agctgcgatt gcaacaggag tcagatctaa tttaccgatc
     9541 acatcaccaa cagcaaaaat atgttcatcc gttgtatttt ggtatttatc tactttgata
     9601 aacccttttt tgtccaattc cacatttgta ttttccagac ctaatttttc ggtattcggt
     9661 acacgtccac cggcaaaaag tacacaatca ccagttaagg tttcaccatt ttcaaatagc
     9721 accgtatatt cgtcattgtt tttagtaatt tctttagcaa tatgatgtgc gtaagttttt
     9781 attccagctt cttggtacat ttggatcaaa ttgtccgaaa gcattttatc aaacgttcta
     9841 agtggtcgtt cttttcgata tgcccacatg acttcactac ctaaaccacg aaatacacct
     9901 gaaagttcag ccgcaatgta tccagcacct aaaacgattg ctctcttagg taactctgtt
     9961 aattcaaaaa atccatttga atcaattgcg tattctccac caggaatggt cattttactt
    10021 gcacggcctc cagtagcgat caagatttta ggagcacgat aggtttcacc gtttacttcg
    10081 atcgtttgac tatcgataaa agtagcgtaa ccttttatag cctcgacatg attgctatct
    10141 aatcctcttt gataagcacc gtgtagaaaa tcaatatatt tttctcgtct ttctactaga
    10201 ccggcgaaat caacattttt taaatctgct tggataccgt aacttgaggc atctcgttca
    10261 attgtttcca aaatcgtact tgcttgccac ataacttttt taggtacaca accgacgttg
    10321 acacatgttc cgcccaattc attcgcttca atcagcaaaa cttttgcgcc atgcatccct
    10381 gctctgtttg ctgaagcaat accgccagag ccgccaccaa ttacgatgta atcatatatt
    10441 tgcattttgt tcctccttat tgaagagatc aatcaaaacc tttatttaac gatccttttt
    10501 ctacagaaaa caagtgtaac acttttcttg aattttgtat gcgaagatgc ttaaaacagg
    10561 gatatatcag ctttttcctt atttacgttt tcggatcata tctccaggtt tcaccggctg
    10621 tgcgactggc atagttaaaa tcatcattgg atttggtgct cggtcaatgg attcgccttc
    10681 ttcattatgc ataacttcaa cggtttgact gaaatgactg aatcctggac cataaaattc
    10741 gatttcatct cctacagaaa acaagtttct ctgacgtatc gtagcaatct gtgttgcctc
    10801 atcataagat aatacttcac cgacaaattt atatactggg attttacgac gttcaccgaa
    10861 taattgttcg ttttcagttg gtgtattgta gtaaaaacct gtagctaatt ctcgttgtgc
    10921 tactttccat aactcgtcga tccattcttg cttgcagaca tagttttcag gatcttccat
    10981 ataactgtct acagccgctt tatagacatt tgacaccgta gatacataat ggatcgattt
    11041 cattcttcct tcgatcttga agctgtctac gccgttttca atcaattctg gaatatgttc
    11101 gatcattgac atatccactg cactcatcga gaattcttct tccacttcgc ctgtatcagt
    11161 taaactgttt cgttcttgac caaaaggcat atcgaacaat ccatacttcc agcgacaaga
    11221 ttgagaacat ccgccacggt ttgcatcccg catcgacata tggttggaca atgtgcatcg
    11281 accagaataa gagatacaca tggcaccatg gataaatgct tcgatttcgg tatccgtatt
    11341 tttacgaatc tctgccactt cctccattga aacttcacga gctaaaacga cacgttccaa
    11401 cccttcttct ttccagaatt ccaatgtctc aaaattagtt gctgaagctt gagtggataa
    11461 atggatcggc agacctgggg cttctgtcgc acaaatttcg atcaaagctg gatcagaaac
    11521 aatcactgca gaaataccga catcacgcaa actgcggaaa aattcccctg ctccatcttc
    11581 atttccttca tgggtgacca tattagcagc aacgtatacc ttcgcatcat gttcttttgc
    11641 aaaagcaact ccttcagcca tttcttcata agtaaaatta cctgcacggc ttcttaaacc
    11701 atatgcattc ccgccaatat atactgcatc tgctccataa tggatcgcgg tttttaactt
    11761 ttccagtgtt cctgctggcg ctaggatttc tggtcgtttc aattttcttt ttactgtcat
    11821 ttctttcatc atactcctta tttgatagca gaaggatcaa tataaaagaa tcctgtatct
    11881 aattcacggt tttcaggatg caatttactg atttcgctag tcattttttc cgctttttct
    11941 tgatcccagc taccttctaa aatttgtttt tttgcttcgg cgaatagtcg agtgatgttt
    12001 acgaattttt cgcctggagt aaataatccg tccaatttcc atgtacagta atgatgctga
    12061 taaagttcat tcaattcatt cgagagattc agatcattgc tggcaaaaat atgtgtgcca
    12121 tgactatctt cataaataga ataatgtgtc tcttcttttt taggttccga gataaaaaga
    12181 cctctttcac gatctttttg ttcgtcttgt ttcgtatagt tatagtaatt ctgtagcagt
    12241 ggacgtttgg attgatggat gcatgtagca ccatacacaa gggtttctac aggaatatcg
    12301 agtttttctt ccatcgcctt catctcttca aaaggaactt cacgagcaag tactgctcca
    12361 actgcgcctt ttttcgccca gaaattgatt tgacgtgcgc tcgttactaa tgtttcagca
    12421 tcataaatat acggtatacg caattcagga ttttttttca tcgtataaat aatacctgta
    12481 tcaccaactg tgatcacatc tacgtttatt tcttttaaaa aagccaaata ttcaggtaca
    12541 tttttcattt tttcaggatg aatgatccca tttacagcaa tattcgcttt tttaccatac
    12601 ttgtgtgcta attctaccaa ttgtcgctgt tcttctcgtg taaatgaagc aggcagacgc
    12661 aatccaaatt gctcttcacc gaaatatagt gtatctgcac ctgcagcaag caactgttct
    12721 gcttgttcaa tagtttctac tgtggttatg agttcgatca taattttctc ctcctacaac
    12781 ttcataatag tagcatagct ctaaaaactt tccaatactt cttctattta aagtcggatt
    12841 tttgaaaatt atttcacaaa aaatcaaaat aaagttcgat atattctgtc caaatataaa
    12901 aagagattgt gacaaaaatc ttgtcacaac ctcttaatca agtcttacta ttataaaaat
    12961 tcagctacac aggccaagta actataggga acctactgtt tcctttttag gtccgcgaac
    13021 attcaatgtg atcttacctt ttgaagcacc tactgttacg tggtcaccta aacgaatttc
    13081 tccgccaagc aaagcctcgc tcaatcgatc ttcaatttct ttttgaagtg cgcgacggat
    13141 tggacgtgca ccatattcag gatcaaatcc agctttcccg atcacatcga ttgcagatgg
    13201 tgtgattttc acttgaatat cttgttcttt caatcgtttg ataatcgctt tgctcatgat
    13261 ttggacgatt gtatggattt catctttact caatgaatgg aagacgacag tctcatcgat
    13321 tcgattcaag aattcaggtc ggaaagcttt ctttaactct tctaagatac gtttttgcat
    13381 ggctttgtga tctttagtaa catcttggac attgaatccg acattttttt cttctcgaat
    13441 ttgtgtcgct ccgatatttg aagtcataat catgattgta tttctaaagt ccacttttct
    13501 acctttagaa tctgttaaat gaccatcatc caatacttgc agaagtaaat tgaaaacgtc
    13561 tggatgtgct ttctccactt catctaagag aatcactgaa tatggttttt gacgaatttt
    13621 ttctgtcaac tgtccacctt catcataccc tacgtatcct ggaggagacc cgattaatcg
    13681 gcttgtactg tatttttcca taaattctga catatcaaca cggatcaggg catcttcact
    13741 tccaaacatg acttctgaaa gtgctttggc caattctgtt tttccgacac cagttggtcc
    13801 aaggaacatg aatgaaccaa ttggacgatc aggatctttc aatccgcttc gtgcacggcg
    13861 gatcgcacgt gaaactgctt tgacagcctc atcttgacca acgacacgtt catgcagaag
    13921 tccttccagt tctaacaagc gttcgctttc ttttttctcc aactgctgta gagggacacc
    13981 agtccactcc gatacaactg tagcaacatc ttcttctgtt acacgatcag aataaccttg
    14041 ctcctgtttt gcttcaattg cagaaacttt ttgcagcttt ttacgtagtg ctttttcttg
    14101 tctgcgtaat tgagcagcat tttcaaagtc ttgtttttgg atcgctgctt ccttttcttg
    14161 tactagttca tcgatttcta gtttgatcac agttgatttt gttaagtgat cggtttgatc
    14221 caaacggact ttcgctgctg attcatcgat caaatcaatt gctttatcgg gtagttgacg
    14281 agaagtgata taacgaacag aaagattgac tgctgctcgt acagcttcat ctgtgatctc
    14341 cactccatgg tgctcttcat atcttgtacg taatccttga agaatcactt ctgcttcttc
    14401 aggagttggc tcatctactt gaatgcgagc aaaacgtcgc tctaatgcag aatctttttc
    14461 gatatatttt tgatattcat ctagggtcgt tgctccgatc gtttgtaatt ctcctcgagc
    14521 taacgccggt ttcaagatat tcgaagcatc aattgctcct tctgctccac ctgcaccaat
    14581 taaggtatgc aattcatcaa tgaataagat tacttgacca tcattataga tttcgtcgat
    14641 gacctttttc attcgatctt caaattcacc gcgatatttc gtaccagcaa ccaaagatcc
    14701 catatcaagc atcatcaggc gcttttcttg catgtcttct ggcacttcac ctaaaatgat
    14761 tttttgagct aacccttcag caatggctgt tttgcctaca ccaggttcac ccaccagtac
    14821 agggttattc tttgtccgtc ggctaagaat ctggataagc cgtttcactt ctctcgaacg
    14881 accaacaact gggtccagct tgttttcttt tgctaatttc gtcaaatcac gtgctaatga
    14941 atctagcgta ggtgttcctt gcggtgtttg ccgtttatct tgtacattta catttctacg
    15001 acgattcatt ccttttccag cagaatcacc agctagcccc attttttgtt tcagaacttg
    15061 acgggctttt gaaaggctga tacctaagtt gatcatgatt cgagtagcca tgatactttc
    15121 atcttttaat aagctcatca aaatatgttc tgttccaact ttttgagaac cagttttctt
    15181 cacttcgctg ctagctaaag caaaaacttg tttcattctt ggtgaatatg gaagaaagac
    15241 tcctttaggg taagaacgga taccaccata accaatcaga tgttcaattt cttcataaat
    15301 ttccgtttca ttcaaatcca tttcacgtaa tacttttcca gcaattccat tttgctcgac
    15361 cactaaggct agtaaaacat gttctgaact aactgattga tgtttcaacc gttttgcttc
    15421 ttcttgtgca atttctaata ctgcttgggc tctttcagta aaaagttctt ccatctaatc
    15481 tcacttccca tcttcataac ttaaacgttc cagaaatgct cggagaatcc gagcacgtgt
    15541 atgatcttca tattcattga aattcaatgt gttttttgca attgcactca acatcaagtt
    15601 tccttctttt tttgtaatta tttggtcttc ataaagtttt tgaataatag caaaagcatt
    15661 tttctcactg attgtatcat caaataactg atttatctgc tcaagtaatt gttttttatc
    15721 tgaaatcctt acttttgcaa ttcgtatata accaccacca ccacgtttgc tttctactag
    15781 ataaccacgt tggatggtaa aacgggtatt aatcacatag ttgatttgtg aaggaacaca
    15841 gttaaagagg tctgccattt caatccttcg aatctcgatc atttcactat tttccagaat
    15901 tttctttata tatgattcaa ttaaatccga ggtattctga tgtgccatct tattcattcc
    15961 ctttgactaa tattgacctt aattataacg aatttgataa tagaaagaaa gaagaacgct
    16021 ttttgttaaa taaatccttt agaatcaaca aaaaaaaata cggaatatac tattccgtat
    16081 cgacgcgccc aagaggagtc gaacccctaa ccttttgatc cgtagtcaaa cactctatcc
    16141 agttgagcta tgggcgcata ttcaatttaa aaaaattata aatcatttga aaacaaaaag
    16201 caagataaaa aaaatcggga agacaggatt cgaacctgcg accccttggt cccaaaccaa
    16261 gtgctctacc aagctgagct acttcccgtt taaaaaataa aaaagactac gcgcccaaga
    16321 ggagtcgaac ccctaacctt ttgatccgta gtcaaacact ctatccagtt gagctatggg
    16381 cgcatatttg aaatgccgag gaccggaatc gaaccggtac ggtgatcact caccgcagga
    16441 ttttaagtcc tgtgcgtctg ccagttccgc caccccggcg cgattgcttt ggcaaagcgg
    16501 aaaacggggt tcgaacccgc gacccccacc ttggcaaggt
//
LOCUS       DACDYJ010000061        16493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDYJ010000061 DACDYJ010000000
VERSION     DACDYJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.061"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..569
                     /locus_tag="IWM51_002043"
                     /pseudo
     CDS             1..569
                     /locus_tag="IWM51_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            627..1541
                     /locus_tag="IWM51_002044"
     CDS             627..1541
                     /locus_tag="IWM51_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971447.1"
                     /translation="MIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRF
                     MIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQG
                     TYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTF
                     KSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFS
                     KISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            complement(1808..2977)
                     /locus_tag="IWM51_002045"
     CDS             complement(1808..2977)
                     /locus_tag="IWM51_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9971448.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            3181..4356
                     /gene="mltG"
                     /locus_tag="IWM51_002046"
     CDS             3181..4356
                     /gene="mltG"
                     /locus_tag="IWM51_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP9971449.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            4492..4971
                     /gene="greA"
                     /locus_tag="IWM51_002047"
     CDS             4492..4971
                     /gene="greA"
                     /locus_tag="IWM51_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP9971450.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            5140..5871
                     /locus_tag="IWM51_002048"
     CDS             5140..5871
                     /locus_tag="IWM51_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP9971451.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            5868..6935
                     /locus_tag="IWM51_002049"
     CDS             5868..6935
                     /locus_tag="IWM51_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9971452.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            6942..7574
                     /locus_tag="IWM51_002050"
     CDS             6942..7574
                     /locus_tag="IWM51_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9971453.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            7714..8379
                     /locus_tag="IWM51_002051"
     CDS             7714..8379
                     /locus_tag="IWM51_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP9971454.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            8407..8700
                     /locus_tag="IWM51_002052"
     CDS             8407..8700
                     /locus_tag="IWM51_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP9971455.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(8768..9715)
                     /locus_tag="IWM51_002053"
     CDS             complement(8768..9715)
                     /locus_tag="IWM51_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9971456.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQLMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(9893..10696)
                     /locus_tag="IWM51_002054"
     CDS             complement(9893..10696)
                     /locus_tag="IWM51_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP9971457.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            10842..11756
                     /locus_tag="IWM51_002055"
     CDS             10842..11756
                     /locus_tag="IWM51_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9971458.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            11804..12274
                     /locus_tag="IWM51_002056"
     CDS             11804..12274
                     /locus_tag="IWM51_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP9971459.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            12359..12967
                     /locus_tag="IWM51_002057"
     CDS             12359..12967
                     /locus_tag="IWM51_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP9971460.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            13101..13301
                     /locus_tag="IWM51_002058"
     CDS             13101..13301
                     /locus_tag="IWM51_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9971461.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            14055..15281
                     /locus_tag="IWM51_002059"
     CDS             14055..15281
                     /locus_tag="IWM51_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP9971462.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(15389..15859)
                     /locus_tag="IWM51_002060"
     CDS             complement(15389..15859)
                     /locus_tag="IWM51_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP9971463.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            16181..>16493
                     /locus_tag="IWM51_002061"
     CDS             16181..>16493
                     /locus_tag="IWM51_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP9971464.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NI"
BASE COUNT         5628 a         2499 c         3196 g         5170 t
ORIGIN      
        1 aaaacatgga aatatttggc ttttatgata cggcatttca cattaatcgt gcgttaagtt
       61 tagaatctat ttttagctcc cctattaatt ttgaaacgtt tagatcatat ggcatgcaag
      121 taaataattt ttacccatgg ttaactctat atcctttatt tttattgatc aagtttacta
      181 atttagcaat aggctataac ctttttcttt atattgttac gctcattact ctatttatat
      241 gtcattatgt aatgtatgaa attaccaaaa aacatgttac tagctccttt ttcgctatta
      301 tatatacaac atcttctttt aggagtgtag aaatatttct tcgaggggca atgggggaat
      361 tactcgcaat gtcaatactt cctcttattt tactcgggtt tataaagctt tacgactcaa
      421 aaaaagaatc ttgggttatg ttggcgatta gcatgacttt attaatttac actcatgttc
      481 tttctgtagc tatgactatg gtaatgatta taattgcttt aattattcaa ttttatcgga
      541 aaaagaaaat agaaatcttc ttaattatta aattaataaa agctgctata gttactttac
      601 ttctttcact agcgtttata ggtcctatga ttgagcagac attgtatcaa gatctgaata
      661 gaccttatgt ggatatatta caaaaaagag caatttatct gggtaaagag tttttgattg
      721 gaagtataga ttcagaatta ctatcatttg gtattggctt aactttgctt atttgtttaa
      781 ttatactaac atttaacttt aaaaaattag gaatcttatc taaaatcact tatataatgg
      841 gtataatatc tattctactt tgtacaaaaa tatttccatg gtttgcctta caaaatactc
      901 ctttaaatat tattcaagct ccttggcgtt ttatgatatt ttcaacattg ttcatatctt
      961 tttcagtatc tctatttatt tctccacagt tggaaaaatt ttcttataca aggagaagaa
     1021 attttatact attattaata ttagctattt caattttaaa ttattcatca gtagttaaca
     1081 tggtgaaaga aatgaatttt caaggaactt attctaatga acaaatggaa aataaacttg
     1141 aagaatttga gaattttgat tatgtaccta taggctataa aaaagatcaa ttgcttttga
     1201 aaaaccatca agttacagaa gatggtcaag ttatttatag tagcttttct ccaaataaag
     1261 atgaaattac atttacgttt aaatcttcaa aaggcaaaac aatagtttta cctgtattcc
     1321 ggtataaagg attaacggtt actgttaatt caaaaaatgc tacagtacta aaacaaggag
     1381 gtccttttgt tacagtaaaa agtgaaagtg gaataaataa agtaacaatc aggtattcat
     1441 acacattttt ttctaaaatc tcattattta tttctataat cacgctaatt gttcttcttt
     1501 tttattgcat aaaattaaaa ataagaacaa gccatgaatg atttgctagc acatatttta
     1561 gcaatctaaa gatactattt tagatttaat aggccctttg tcaaataatg ttggtaaaga
     1621 gaaatcttag aataatttgg acagaacaga aagaagaaca cctctttctg ttctgtttat
     1681 ttggaattaa agtctgtagc aaaaattgat ttttagattt gtcccacact ctttcctacg
     1741 tttagtcgag tacttttttt aagcaaatgc tattcaaaga agtagtattt gctatctttt
     1801 catcatatca gctatttgcc aacttagtag ttccttccat caggtcatag atttggtttc
     1861 ttagatcagc agtaggcagt ttgatagttt tgtcagcttt tatattgctt tccatgatga
     1921 tagcatcctg tgaatctttg ctaaatatag cagatgtatc aaaagagtcg atatttcctt
     1981 gcgacaggat ataattatct ccgctatata ccccaccact atatggatgt ccattcgcat
     2041 tttcacgcca taatgagaag agtaagtctt tggaaactag ttttccagat aaaacttgtg
     2101 taaacagcgt atttaaatca tagacggaca tactgatatt accagtccct aattcgttat
     2161 ggatctctgt ttcagattct gtcagtggtt taaagtaatc atcctcttta gacatttgat
     2221 aggatagtgc atgattggat gaatttttga cttggtcata aaattttgta tgctgtaatc
     2281 ccaaaggttc gatgatatca tcggtcaata atttttcata cggttgattt gttaatttga
     2341 tcaaaattcc agtcaatatg gtaaagttca atgaatcata atgagaggtc tgataatctg
     2401 taaattttaa gtgctgaatc gcaaattgaa cgacttcttc atcagtcatc ggtatggttg
     2461 gagaagcagt tcggtgcaag cctgagcgca tatacagcaa gtcacggata gtaatttgct
     2521 ggctgccagc aatttgtgga tagaattgag ccagcttagt atctaaagaa aggtgcccac
     2581 gctcgacttg ttttaaaatc aaaagagtag tcaatgcttt ttgaaccgaa ccaatctgat
     2641 aataaagaga aggatcattt ggttgttgcg tgagtttatt cgcatagcca tagccttttt
     2701 ccatgatgat gcgattattt ttctttacgt aaattgagcc aatataatca ttttttctta
     2761 atagttgatc gattttttca acgagctgtt gttcattggt agtcagtatc aacttttttt
     2821 gagctgattg agcatgggca actatctgag agtgttcatt attgtaaaat tgccatccaa
     2881 gaatagttcc tgtagagaac gcactaaggc taaacaacac gacaacgatc attaaaaatg
     2941 aatattttct ttttttagca tgttttcctt ttttcataat agtttattta gtctcctttc
     3001 ttttttacag aaagaataaa gctaaaatag cttgtatctt gataacattt attttaatct
     3061 tatagtaatg gatttactac ttacattttt gatagaatat ttaaataaaa tcattcacgc
     3121 caattcaaat atcatgtata atgaataaag ttacgaactt gatagtaagg aggagttgtt
     3181 ttgtcaggaa acaaacaaca cccaagtaaa gataaaaaag aagccagtaa ggcagctaca
     3241 gcagatataa tcgacaagcg gagaaatctg cgtaaaaaag aagataagat tgtgaggaag
     3301 atcattttag tgattgctct tacattattg attattggcg gatttcttgg atttaccgtg
     3361 tatcgttatg tagatagcgg gttgaagcct ttagataaat cagatgacca attagtccaa
     3421 gtcgagatcc cgagtggctc ttctaacaag cagattggtg agattttgga gaaggacaat
     3481 attattaaaa gcgggattgt tttcaattat tatacaaaat ttaaaaattt aactggcttt
     3541 caagctggct attatcagtt agcgcctaat atgactcttg acgagattgg caaacagttg
     3601 caagaaggcg gtacttctga gccaacaaaa gttgcagatg ggaaaattgc cattccagaa
     3661 gggtacgata tcgatcagat tgctgaacgg gtagcgaagg taactggtaa agacaagaaa
     3721 gaatttttag atttagtgaa tgatgagact ttctttaacg agttgcacca aaaattccca
     3781 gaactgttgg aaagtgcatc taaagctgag aacacaaaat accgtttgga aggttactta
     3841 ttcccagcca catatgatta ttacacgagt acttcgctta aggatctagt gattgaaatg
     3901 gtgaacaaga cgaatacggt catgcaaaac tattattcag ctatcaagca gaaaaatttg
     3961 actgtacaag aagttttaac tctagcttct ctagttgaaa aagaaggcgt aaaagaaaat
     4021 gatcgtaaaa acattgcaca agtcttcttt aaccgaatca aagcaaatat gccacttcag
     4081 tcagatatct ctgttttata cgcattaggt gaacacaaag aattagtgac gtatgaagat
     4141 acagctgtag attcaccata taatttgtat accaataccg gctatggacc tggacctttt
     4201 gacaatccaa gcgaagaggc aatcaaggca gtattagaac cagcagaaaa tgactattat
     4261 tatttcgtag cagatacgag cactggaaat gtttatttcg ctaaaactta tgaagaacat
     4321 atggaattag tggaaaaata tgtaaataat tcttagaaaa acaggctcta gacattgatc
     4381 atttttagaa aaacatttac atttgaagaa aagcgtggta atcttttttg gactatattc
     4441 ctcaaaagat taccttttgc ggtattttat cgaaaaaagg agacgttaca catggtagag
     4501 aaagtttatc caatgactct agaaggtaaa gaaaaattag aaaaagaatt agaagaacta
     4561 aaaacagtca aacgcggaga aatcattgaa cgaatcaaaa ttgcccgcgg tttcggagat
     4621 ctttccgaaa actctgaata tgaatcagcg aaagatgaac aagcatttgt tgaaggaaga
     4681 atcacaacaa ttgaaaatat gattcgtttt gctcaaatca tcgataacga cggtgtagat
     4741 gcagatgagg tatcaattgg taaaacagtt acattcatcg aactaccaga tggagaagaa
     4801 gaagagtaca cgattgtcgg aagcgctgaa gcagatccgt tttctggcaa aatttccaat
     4861 gattctccta ttgcgcaagc attgattggc aaacgtaaaa atgatgaagt aactattgct
     4921 actccaggtg gcgatatgtt agttaaaatc gtcaaagtag aacaagcgta gtctgactga
     4981 aagatctaga gagttcatct tactgatgat acactctaga tctttttctc tttgtgtatt
     5041 ttagatttca acaaacgatc ttacatctaa agtcctatag ccaacagtta aaaaaatagg
     5101 tatgatgaaa tgagaatgtg tcaggaggga gaaagagcga tgaatagttc ttggcgtttt
     5161 tttgtggtag ttgaagcact actgctgtta tttgctgttt ggcaaatcgt aaacaatact
     5221 ggacttttgc ttttagtgat attcggtatt ttcaatatat atctagctat gcgcaaacat
     5281 cctcgtacta aatttcaaaa ttttcaattg atattaggaa gtttggtcat tttttttagc
     5341 ttagtcaaca gtcctgcttt atggatgatg gctgtatttg ccgttttatt tattggtctg
     5401 aaaggtgtag agatttcagg gatagattta accaaaaatg ctttttggcg aaagaaacag
     5461 atcatgatga tccaaacaga gcaattaaaa acacataata atgagcggaa aagacagcaa
     5521 ttattcggta accaacgtat tggaaacgac gtctatgaat gggacgatat caacatcgcc
     5581 gtcatttctg gtgatactat catagatttg ggcaatacgc ttttgccaaa agatgataat
     5641 attgtgattg taagaaaagg gataggcaga acacggatct tagtaccttt gggcgtagcc
     5701 atccgattag agcatgcgac attagtagga aatgtcttgt ttgaagaaga gcaattttct
     5761 ttgaaaaatg aacaaatcaa aatctacagc agtgattatg atgaaaaccc tcgtagattg
     5821 aagattatta ctaatacctt gattggagat gtcgaggtga tacgaatatg atgggaaaaa
     5881 tatccagagc gatgctagct gtttattcgg ggattgcggc tttccttatt attctatttt
     5941 cacttttcac ttatttttat gccagcaatc aaagtcattg gtggggagaa ttactgcgtg
     6001 cacgtttatt atatgttccg cttatcttcc atttgttagc catttcctta ggtgtaggat
     6061 taattgtctt tctattatta tcacttattc aaaagacaaa atacgggaaa atcgaagaaa
     6121 agctgcgtgc actttcttct ggcaattatg aatccaaatt gttgcttctt ccgatcccaa
     6181 gtgcatcgga cgatttatac atcaaagata ttgataagga aatcactaag ataaaagaaa
     6241 aaatgattga agtatctagt gaattacaaa ttgtaacgag ccgtccccaa tatgtagatg
     6301 gacaaaccaa agaagaaatt ttagaattag aaagacatcg attagcccgt gagctgcatg
     6361 attcagtttc gcaacaattg tttgcagcaa tgatgatgat gtcggcattg acggagcaag
     6421 cagaaaaaag cgagacacca gaaatgtttc gcaagcagtt gaaaatggta gcagagatca
     6481 tcaacgcttc ccagtctgag atgcgtgcgc tgcttcttca tctacgtcca gtcaatttag
     6541 aagagaaaag tttaaagcaa ggtatcgagc agttattgaa ggaattgcaa aataaaatcc
     6601 agatttcgct gaaatgggat gtagaagatg taaaactatc tagttccatt gaggatcact
     6661 tgttccgaat cgttcaagaa ttgttatcaa atacattaag acatgctaaa gccaatgaat
     6721 tagaggtata tttgcacaaa atagacaata atcttttgtt acgtattatt gacgacggaa
     6781 cagggtttaa tatgaatgaa acgaaaacgg gaagttatgg attgaacaat atcaaagaac
     6841 gagtagctgg tattggtgga acagtaaaaa tcattagttt taaaggtcaa ggtacaagtg
     6901 tagaaatcaa ggtccctttg atgaaggagg catagataag gatgataaaa gttttattag
     6961 tagatgacca tgaaatggtg cgcttaggcg tctcttctta tttatctatt caagaagaca
     7021 ttgaagtaat tggagaagct gaaaatggac gacaaggcta tgagaaggcg atgacacttc
     7081 gaccggacgt cattttaatg gacttagtca tggaagaaat ggatggtatc gaatcaacaa
     7141 aagcaatctt aaaagattgg ccagaagcta aaatcattat cgtaaccagt tttattgatg
     7201 acgaaaaagt ttatcctgcc attgaagcag gtgcagcagg gtatttgttg aagacctcta
     7261 ccgcacatga aattgctgat gctataaggg caacgcaacg tggcgagcga gtattagaac
     7321 cagaagtgac aacaaaaatg atggaaaaaa tgagtcggcg aaatgagcct gtattacatg
     7381 aagaactaac gaatcgggaa aatgaaattt taatgctgat ttctgaagga aaaagtaatc
     7441 aggaaatagc ggacgaattg tttattacac tgaaaacagt aaaaacacat gtttccaata
     7501 tattggctaa gctagaagtg gaagaccgta cacaagccgc tatctatgca ttcaaacatg
     7561 gtttggtgaa ataaaaacga tgataaagag gataatcaaa agctgagaaa ataactatta
     7621 tttttcttgg cttttttaca ataatgatgc ttgcttttgt ttttgtaaag gctttgttat
     7681 actgagaaaa gaagatacta aaggaggaaa acggtgaaac aaaattttgc aattgtaggt
     7741 cttggacgat ttggcggaag tatctgccga acactgatcg aagctggtca agaagtactg
     7801 gctattgata gtaatgaaga tcgggtcaat gaatatatga atatagccac tcatgctgtt
     7861 gtggcaaatg cacaggatga aatgacactt cgatcattag gcataagaaa tttcgatcat
     7921 gtaattgtcg ctattggaga agatattcaa gccagtatct tagttactct gatggtaaag
     7981 gaaatgggcg taccaaatat tttagcaaaa gcccaaaatg agtatcatgc cagagtactt
     8041 gaaaagatcg gagctgatcg cgtcgtccat cccgagcgag acatgggaat aaggattgcg
     8101 cataatttag tgtcaaagaa tattttagat tacctagagt tgtcagataa atattcttta
     8161 gcagaaatcc gagtctccaa tcctaaattt ttcaacaaaa cattagctga attaaatttc
     8221 aggcaacgtt ttgacttaac agtagtcgcc atacgaagag tagatcaaac ggttatcgct
     8281 tctcctgcag cagatgaata tgttcgtgaa aatgataatc tactggttat tggagagaca
     8341 ggcgatgtgg atattctaga cgataagatg aatcaataga aagaaaagag aaagtaggtt
     8401 gttgggatga aattaacggt aacgccaaaa gcacaaaagt ggtttgactc agaaattgat
     8461 ctgccagaaa cttacggaat acgttttttt ggaaaagtct acggcaaaac agaggttcat
     8521 gatggttttt cgattggaat gtcagttgaa cagccagaac atcctgtaaa gaaagtggaa
     8581 attgatggga cacttttttt tatcgaggaa gcagatgaat ggttctttaa aggatatgat
     8641 ttacttgttg attatgatcc caacctggag gagccaacct atcactttaa aaaacaataa
     8701 aaaaagagag ggtgacacaa tttttgtcac cttctctttt ttcttttgaa aatattgttt
     8761 tcagctatta atatcctgca gacaatttta cttgattggt ttgaagctca tgggtggcaa
     8821 agtagctttt cagattggtt aaaaaaatag ccattaattt cttttgaaaa tcaggtgtca
     8881 aacctgcaat gtgggaagta atcagtacat tgtccatttt ccaaagagga tgatcttgtg
     8941 gaagaggctc ttcctcaaaa acgtctaatg ctgcaaagga aagttgctgg ttatttaaag
     9001 cctgcaccag atcttgtgtc ttaacagaag caccacggcc aacattgatg aatacagatt
     9061 ttggatcaaa gtgttcaaac ataagctgat caaagagccc agttgtttcc tccgttagag
     9121 gtaagatatt gactacgaaa tctacttcag gaagaacttc ctttagttga tttaaagaaa
     9181 aagtcttttc cattccttct gctggatgac cggatgtatt gattccatag cagttcatgt
     9241 ctaatgcttt gcattttttt gcaatcattt ttccaatatg gccagttcca atgattagta
     9301 tttttttccc ttggatagat tggtaatgga ccgatgaacc taaccatttc atgtcactct
     9361 gtgccttttg tgcttgaaaa agaccacgag aatacccaag gatgattcct aagacatgct
     9421 ccgtaatcga ttcactgtgg atgccactac catttgaaag taaaatatgt ttctttgaaa
     9481 aagtctcaag gggtagctga tcaacaccag ctgagataga ttggacccat ttcaaggtag
     9541 aagaggctaa tagtttttct tggtagtctg agttccagcc caaactgatc tctacatttt
     9601 gtagatcctc tggttgtaat tctgttttaa agatataacc aggcgctata gtatggattt
     9661 cttcgataaa ttcatcacga aatggacgat ctgcataaat gattctttct gacatagtta
     9721 atttcccgcc tttctttact ctaagtgtag cgcatacaag aagaactttg aagcgccttg
     9781 cttgtttttt gagaaagaaa tatttttcaa ataaaaaagg ggctgtgaca aaaaaatgtc
     9841 acaacctcta aaagggtcta ttgaagtaga aataacgagt tgtctttatt tcttaattga
     9901 tcgatatttt tgcccatcct ttgacagctt gagcaaaaat aaagattcct gcaaaagcaa
     9961 tgaaggcgct agtgtcaaaa gaaggaagaa agaccatcaa gattgttgca attatagtag
    10021 gcaatgaaat tgaatagatc aatgttttta aacaatcaag aaaagtagct tttgttagtc
    10081 gaaagcgtgc atagagaaaa gcagcaatgt tggtaaatag caaagtgatc agcagattca
    10141 gaaaactagg ataaatagaa aatataaatg tcaggacttt tatccaaagt ggtatagatg
    10201 cttcacttaa aaaagaacgg agctgcttac ctgttaagtt ttttaaagat tcattggtgt
    10261 agggaagttc taattggtta ttgttcaaca aagcagaagt cgtgcctgta ttaggaagag
    10321 ctatgataag tttgtttttt aacatcccga cactaacaaa attccccatc aaatcagtag
    10381 agatgtcctg ttctgttcgt tttccctctg gatcaaatgt gaagataatg gagtttgttt
    10441 ggtaaatgaa tccttctttt tcttcggttt gaagctgccc gttttggatt gtaaaatcag
    10501 gaatgcgagt agcaatcttt tgcccatcca attgaatgtt gtttaagact tgaaaaactt
    10561 gaaaagagat tggcaaagcc ataattatac tcaaaactaa aagatagaca atggctttcc
    10621 aaaatgggac atttctcgcc tcctttaggt ctttgaatcg tataaaggaa ctgataatta
    10681 attgttttgt tgtcataagt actcctcatc tagtagtgtc atcacttgca attctagcga
    10741 agtatcaagt gaaatacaag tgctttaagg aaacagctga atgattggtg aaatatcaaa
    10801 aaaatgttat tcttgtctaa agactatcca atataaataa aatggaggaa ggaatagtgc
    10861 ctacacagcc acagagatca tcaaaaaaac tatttggagt agtggttttg aatgcggtga
    10921 tcactttatc agaatttatt ggagggatta tgtcagggag tttgtctctt gtttcagatg
    10981 ctttccataa ccttagtgat acattggcaa ttattttttc ttacggggca caaaaaatgg
    11041 cccgaaaaga agcaaataaa aaacgtacgt atggctacca gcgtttagaa atattgagtg
    11101 ctttcatcaa ctcattcata ttgatcatat tatccttgtt tttaaccgtt gaagcattca
    11161 agcggttcac ttcaccagaa aagataaata gtcatttgat gctaacagta gcagttattg
    11221 gattgctcgc gaatttgttc tcagtattat tattacgaca agaagcagat gaaagtttga
    11281 atataaaatc tagttatttg catttgttaa gtgatacact gtcttcggta agtgtgatta
    11341 tcggagcaat tttgatccgt tttttcgata tctactggat cgatccagtt attacactga
    11401 tcatttcgat atatatattg atagaagcga taatggtgat caaaaaagct gccgctattt
    11461 tgatacaaag tgcacctgct atcgactatg aaaaaatgga acaagaaatt gaagcaatcg
    11521 atggggtaaa ggatgttcat catgtccata tttggcagta tagcgaaaag ataattattt
    11581 tcgacggaca tattgatttt gaagatcagc tactaagcga gattgaaaag atatatcctc
    11641 gtataatttc tttattaaaa ttaaaatatg gtattacaca tgtgacgatc caagcagaga
    11701 caaaggcaaa ggatcaaaaa aaattaattt ttttaaataa agatgaaagt tattgaaata
    11761 tagagtatga taaataagaa tttttaataa ggtaggttgg ctcatgaagc agtatcgaca
    11821 gttaaaagga tatttagaag caaaaggata ttgggagata ctagtctatc tcttttttgg
    11881 tggtttggct acgattgtaa attttgttag ttttgctctc gcacgccaat attttgattt
    11941 aagtatggcc ttatccaatt ctatttcgtg gttttgttct gtattgtttg cttttgtaac
    12001 gaacaaactt tgggtattcc attcaaaatc accgaatttt acacatgcac ttattgaatg
    12061 cggtaagttc ttcttctacc gtatcctttc ttacggattg gacatggggg caatggtatt
    12121 gttgatcaat gttatgaaca gcaatgagta tgtggcgaaa atcatcactc aaattattgt
    12181 tatccttgcg aactatattt tcagcaagtt gtttattttt aaagaaactg aggtttttga
    12241 agaagaagta ggtcaaagtg aaaaagacga ctaggcataa caattgaaga atttccttct
    12301 ctttgttact ctttgtaatg tagacattat taagaaaaca ttgaggagga atttttttat
    12361 gacttacaca ttaccagatc taccttatgc atatgacgca ttggaacctt atattgacga
    12421 agaaacgatg catctgcatc atgataagca tcacaatact tatgtgacga atttaaattc
    12481 agcaattgag aaatacccag aattaggcga aaaaacaata gaagaattat tatctgatat
    12541 ggacgctatt ccaacagata tcaagacagc tgtacgtaac aatggtggcg gacatgctaa
    12601 ccattcattt ttctgggaaa tcatggcacc aaatgctggt ggcgaaccta caggagaaat
    12661 aaaagaagcg attaatgaag cttttggtga tttttcttct tttaaagaag aattcaaaaa
    12721 agcagccgct ggacgatttg gttctggatg ggcttggctt gtaatggaaa atggaaaatt
    12781 agctattacc tctactgcaa atcaagattc tccattgatg gaaggaaaga caccaattct
    12841 aggtttggac gtttgggaac atgcttatta tttgaaatat aaaaatgtcc gaccagatta
    12901 tattgcagca ttctggaatg ttattaattg ggatgaagta aacaaacgtt tcgaagcagc
    12961 aaaataacca tttcctccgt catatcagtc ttaaaaataa tttggaaaat aaaagagcga
    13021 aagcttgcta gcgttttcta aattgggtat aatgttcttg caaggttact tgcaagaact
    13081 tttttggagg atatacattt atggaacacg gaacggttaa atggttcaac aatgaaaaag
    13141 gatttgggtt tattacagta gacggcggag atgacgtatt tgttcatttc tcagcaattc
    13201 aaggagacgg cttcaagagc ctagaagaag gtcaagaagt agaattcacg attgttgaag
    13261 gtgcacgcgg acctcaagct gctgaagtga ctaaattata ataatttgtt gaataaagac
    13321 acgacttctt agagagtcgt gtctttttgt ctatttatga agtttttaga ttttttggct
    13381 aagtaaatgc atttgttcac ttgacatgtc ctaactaatt atcaagcaag gaacattttc
    13441 ttcgttagta atggatttta aagaaatttc agtttttgga tgttgaataa aagaccattt
    13501 gtgaaaccac tagtatacat actcattacc atgtcaattc tattacttta aaagaaaatc
    13561 gttttacttt gaagaggagc atatctatcc attaaaagat gatggtaatg caatcactag
    13621 ttcgagtcat tttaagagcc atgcagtgag ctggaaataa aacgaataga agatgggttt
    13681 aacaaaagga ccagtcatag gaagaaattg tcgtgaaatc gaatgataat ctgaacggta
    13741 ggcaaagact gttaaaaaga tgggaaaggt atgtgcgatc attttactgc acgattattt
    13801 ggaaaatacc ttgttgttcg ctttttctgg aaaaaataca taaaaaaaga taaaacgatt
    13861 gcgaactgcg tgtattcggt ctttcgatct ttttcacggt atggtatgat agttaagaat
    13921 tttgagaaat tttaaaaaat aataatcgtt acacaaaaat gttttttaaa gttttcttat
    13981 gattaaaaag tgaatttgct gattgaacag ttatatgaga caatatttta gaaaatgtga
    14041 aacagaggtg tattatgaag cgatggcaaa aagttctatt aaccttgtta ggggtattcg
    14101 ttttgctgat tgccggatta tcagcatacg gaatcagatt tatgggagaa gcgagccaaa
    14161 cagtaaacaa aatttctaaa aactctaatc gaatatcctc caaacgttct gagagagtaa
    14221 gcattgatga caaagaacct ttttcggttt tgctattagg tttagatacc ggtggattag
    14281 gaagaacgga acaaggacgt tctgatacga tgatggtagt aacagttaat ccgaaacaaa
    14341 aaaaatcaac gatcatcagt ttggaccgag atatttatac gaatatcgta ggttatggaa
    14401 cggtagataa attaaatcat gcctacgcat ttggtggtgt cgaaatggcc atggattcaa
    14461 ttgagcaact gctcgatatt cctattgatc attatgtaac gatcaatttg gatggcatgg
    14521 aagatttgat caatgcggtc ggtggcatca aagtgaataa taaaattgac tttactttgg
    14581 atggtgtcca tgtaccagta ggtgaacaaa cattgaatgg agaaaaaggt ttagcctatt
    14641 ctcgaatgcg acacgaagat ccagaaggcg atatcggtcg acaacgtcgt cagcgggaag
    14701 tagtgacgaa aatcgtcaat aaagttttaa gtttagatgg cgtaagcaat tatagaaaaa
    14761 ttttaaaagc agttgagaac aatgtgacga cggacttaga ttgggatgac atgcttgatg
    14821 tagcgacgaa ctacaccccg gcgtttgaaa caatcaaaca agaccagttg caaggcgaag
    14881 gacaaatgat caatgatatc tactatcaaa ttttaggaaa aaatgatctt ctttcgatcc
    14941 aaaatgaact gaagaaacaa ttaaatataa aaacaagcga tacattacct aatttgaaaa
    15001 atgagaatgc ttcaatgatg ttctttgatg attcggaaga tggcgattcg acaaacggtg
    15061 atggatcgaa tagtgaaggg tacaccgatt attcttccta ttcagaaaac acatatgatc
    15121 agtaccaaga tccgaatacc tattatcagc aaacagaaga tacaagtcag tactatacac
    15181 caagtcaaga tactcaaagt tatgatcctt acgctggaca ggaacaaaat ggttatggtt
    15241 atgaccagaa taatgagtat ggaacaacaa gtgggtacta gaaaaaaata gtttggttaa
    15301 taaagagtgc cacctaaatt agacaaaaaa tctaacttag gtggcacttt ttctatgtgt
    15361 taaattacag gacgtctctt tttagcgctt agtttaaaaa ataaattgaa taattcaata
    15421 gagttgcgaa tagtacccat aaataataag gaatcatgag ccacattgcc aggcgatgat
    15481 gcagccacaa ctgatacata agcactgaca agaaaataac taaaagtgta atatcgattg
    15541 ctgcaatgaa aatatttctt aaattaaaaa atagagcaga ccatataaaa ttaaaaatga
    15601 actgaatcac aaaaagaccg acaagattgt ttcgctgttt tttgttgttg tgattaagta
    15661 acaaataaag agaaataccc atcaatatat acagtaaggt ccacatcggc ccaaatatcc
    15721 agctaggtgg tgcaaatggt ggaagttgca aagaatagta atattttccg ggatttccgc
    15781 taagcaaccc tgataaaaag cctaatatga caattcctat aacacaaacc caaaaacgaa
    15841 tatcttttat catcttcatt ttactaactc ctttctataa tattagttta ccatgaaggc
    15901 tcacagcaag acctgtattt agtttgtgaa gtaagaaaaa aattgataat tgttgttatt
    15961 aaaaagttga acatttgttt atatgaatga tgtttttgaa aaagatcata tgatttgatt
    16021 ctcatgtttt ctatttgtac ttttctcgtc tacttctgtt ataatctgca tcttactttt
    16081 ttcacctttt ttacttcaac ataaaattat aaaagcataa tgtttgtgtt aatacaataa
    16141 agcgtttaca atgtggttat caaatgaagg aggtttctac atgaaaaaga ttagtacgtt
    16201 attgtttatt gggttagtta gtttagggtt gttcggtttt agcattccag gttttgcgca
    16261 tggttatatt acttctccag gaagccgtgc atacctagga acatcagcag caggaaactt
    16321 aaatcaaaat gtcggaagag cacaatggga accgcaaagt atcgaagcta ccaaaaatac
    16381 attcattgat ggaaaaattg ctagtgcagg agtcagcggc tttgagcctt tagatgaaca
    16441 gacatctaac agatggcata aaaacatggt taatccagga acattaaata tta
//
LOCUS       DACDYJ010000062        14627 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDYJ010000062 DACDYJ010000000
VERSION     DACDYJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.062"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            333..947
                     /gene="gmk"
                     /locus_tag="IWM51_002062"
     CDS             333..947
                     /gene="gmk"
                     /locus_tag="IWM51_002062"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP9971465.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            952..1257
                     /locus_tag="IWM51_002063"
     CDS             952..1257
                     /locus_tag="IWM51_002063"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9971466.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1510..3918
                     /gene="priA"
                     /locus_tag="IWM51_002064"
     CDS             1510..3918
                     /gene="priA"
                     /locus_tag="IWM51_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP9971467.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3933..4424
                     /gene="def"
                     /locus_tag="IWM51_002065"
     CDS             3933..4424
                     /gene="def"
                     /locus_tag="IWM51_002065"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9971468.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4417..5355
                     /locus_tag="IWM51_002066"
     CDS             4417..5355
                     /locus_tag="IWM51_002066"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP9971469.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5355..6713
                     /gene="rsmB"
                     /locus_tag="IWM51_002067"
     CDS             5355..6713
                     /gene="rsmB"
                     /locus_tag="IWM51_002067"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP9971470.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6726..7466
                     /locus_tag="IWM51_002068"
     CDS             6726..7466
                     /locus_tag="IWM51_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP9971471.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7463..9532
                     /gene="pknB"
                     /locus_tag="IWM51_002069"
     CDS             7463..9532
                     /gene="pknB"
                     /locus_tag="IWM51_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP9971472.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9697..10596
                     /gene="rsgA"
                     /locus_tag="IWM51_002070"
     CDS             9697..10596
                     /gene="rsgA"
                     /locus_tag="IWM51_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP9971473.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10609..11259
                     /locus_tag="IWM51_002071"
     CDS             10609..11259
                     /locus_tag="IWM51_002071"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9971474.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11260..11898
                     /locus_tag="IWM51_002072"
     CDS             11260..11898
                     /locus_tag="IWM51_002072"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP9971475.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12053..12907
                     /gene="mreC"
                     /locus_tag="IWM51_002073"
     CDS             12053..12907
                     /gene="mreC"
                     /locus_tag="IWM51_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP9971476.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12911..13420
                     /gene="mreD"
                     /locus_tag="IWM51_002074"
     CDS             12911..13420
                     /gene="mreD"
                     /locus_tag="IWM51_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP9971477.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            13675..>14627
                     /locus_tag="IWM51_002075"
     CDS             13675..>14627
                     /locus_tag="IWM51_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971478.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESS"
BASE COUNT         4910 a         2714 c         3182 g         3821 t
ORIGIN      
        1 gtattaagcc aaatttatgc aaaaatgtga gaaactattt tcacaaaatt tttcttcata
       61 ctcgaaacta atcggcttct tcacgacctc ttatacacgg cgttcaaaag cttgcatctg
      121 cgataataac cccaaccaaa cgaaaaacat gagaagctgt ttatgtttgg ctgttcttat
      181 tacttgatgc aggacagctt ttttacgacc tcttggtaca gggtagcttt tttaccaatt
      241 tctagctata attaaagata accttgaaag ttgcagaaaa aaagtgcaaa ataaacgata
      301 agtacaaaaa gactagttga aaggacgtcg tcatgtcaga gcgtggatta ttaatcgtgt
      361 tgtctggtcc atccggtgta ggaaaaggca cagtacgaaa agcaattttt gagagtgagg
      421 ataatgattt tcagtattcg atctcaatga ctacccgtaa aatgcgtgaa ggggaggtcg
      481 aaggcgttga ttatttcttc cgaagcaaag aagagtttga atcaatgatc gaagctggag
      541 agatgctgga atacgcagaa tatgtcggga attattatgg aactcctttg tcatatatca
      601 acaagacatt ggatgaagga aaagatgttt tcttggagat tgaagtccaa ggagcaaaac
      661 aagtcaaaga aaaagtgccc gatggtgtat tcatcttttt gactccgcca gatttagcgg
      721 aattaaaatc acggatcgta ggccgaggaa cagatgcaga tgaagtaatc gaagaacgaa
      781 tgaaagttgc aaaagaagaa atcgaaatga tggctcttta cgactatgct gttgtaaatg
      841 atgaagtgcc gctagcagtt gaacgaatca aaaacattat tgccagtgaa cattttcgag
      901 tagatcgcgt aatcggtaaa tatattaaaa tgttaaagga gatgtagtct tatgatgtta
      961 aaaccttcaa ttgactcact attagatcgt gtaaattcaa aatattctct agtgatttta
     1021 gcaagtaaac gcgcgcatga attggatgca agtgcacagc ctactctaga ttcttttgat
     1081 tcagtaaaaa gcgtcggtca agctttagaa gaaatcgatg caggaaatgt agtcaatgat
     1141 ccacatcctg aattaaaacg tgaacgtttg aagatggaag aagaggaacg tcaagcacaa
     1201 aaagagcgtg aacaacacga attggaatca cgtatccgtg aagagcaaaa aatgtaataa
     1261 agaaaaatag agcgaacatc taaaaagaaa agcaaaccca gctgtttcct tttcaaacat
     1321 tcgccactta ttgtctggta gaaaaggctg tgtctgaact ttttgacaca gttctttttt
     1381 ttcttatctg ctgttaattt tttgttagga tttggcagaa gataagacaa atccatcgat
     1441 ctctagtaaa ataggcaagg aactttttgt cgcttgataa gaaaagacaa aaaaaggagg
     1501 aaacgaacca tgacattaac agctgaagtc attgtggacg tacctactat gcagaccgat
     1561 cagccattca cttatttggt tccctctgaa atggaaacag ctttgcaagt cgggatgcga
     1621 gtagaagtcc cttttggtaa tggcaatcgt catgtccaag gatttgttat ggctatccaa
     1681 aaatcagaag aatcagctaa tccatctttg aaggcagtta ttcgtttgct agaccttgct
     1741 ccggtggtca atgaagaatt actttcatta gctgattata tgaaaaaaat tacttacgca
     1801 ttcaaaatca cttgtctaca aacgatgctt ccgagtgtga tgaaagcaga atatgataaa
     1861 ttgatctatc ctttggcaga cacaccagaa gttcaggtat tgtttgatca gcgagaagtg
     1921 atttcctgga aggaagcaga agaagaaggc agcctttctc aattgattcg ctggcgccaa
     1981 gagcaattag tagatatcaa atatgaagtc catacacgaa ataaagtaaa aacgattcgt
     2041 ttggtccgtt ctttgttaac tgaaaagcaa atcgaagaag aatgggcaaa attacggcaa
     2101 aatgccaaga aacaaaaaga acttcttttg tgtttatctg aactgagcca agaagaacca
     2161 attgcttatt tcaaaaataa aggaatcagt actgctgtct tgaatcaagg aaaagaaaag
     2221 ggctggctgg agtttataga atcagaacgt tatcgcgacc cttataaaga ccgagtattt
     2281 gaccaaacga cggcattgga actgaatgca gaacaaaaaa atgctgtcga acaaatcatc
     2341 acagcaggtc agcagcaaaa agatcaggtc tttttgctgg agggaatcac tggcagcggg
     2401 aaaacagaag tatatcttca ggcaatcgct gatgttttgt cagaaaataa aacggctatt
     2461 atgttagtgc ctgaaatcgc attgactccg caaatggtcg agcgattcaa aggacgcttc
     2521 ggagaatcag ttgcggtctt acatagcgga ctttcccaag gtgaaaaata tgatgaatgg
     2581 cgcaagattg agcgggaaga agcgcaagta gtagtaggcg cacgatctgc tatttttgcc
     2641 ccgctgaaaa atatcgggct gatcattatc gatgaagaac atgaatcaag ttacaaacaa
     2701 gatgagacac cgcgttatca tgcgagagat ctagcaattt ggcgcagtaa atatcatcat
     2761 tgcccaatag ttctaggaag cgcgacgccc tctttagaat caagagcacg agctcaaaaa
     2821 ggcgtttatc agttattgca gttaaaccac cgagccaaag cagctgcaca gcttccagct
     2881 attgagatcg tagatatgcg tgaagagttt cagaatcatc ggacctctac tttttctgct
     2941 aatttgcagg aaaaaataca aaatcgatta gacaaaaaag aacaaactgt cctactgctc
     3001 aatcgtcgag gatattcgtc ttttgtgatg tgtagggact gtggatttgt tcttccatgt
     3061 cctaattgcg atatctctct tacactgcat atggatacac gttcaatgcg ctgccattat
     3121 tgcggacatg aagaacctat tcccaatcgc tgtcctaatt gtggcggcaa taaaatccgc
     3181 tactatggaa caggtactca aaaagtagaa gaggaactcc gagaacttta tccgcaagct
     3241 aggattttgc gtatggatgt agataccact cgaagaaaag gggcacacga acagatttta
     3301 caaaaattcg gtgcaaaaga agcagatata ttattaggga cacaaatgat tgctaaaggt
     3361 ttggactttc cagaagtgac attagttggg gtgttgaatg ctgatacctc cctgaattta
     3421 ccggattttc gttctagtga gcatacattc caattgctta cccaagtttc aggacgagca
     3481 gggcgagcgg aaaaagcagg cgaagtcgtg atccaaacat tcaatcctca acattacgcc
     3541 atagaattag ctaaaaaaca aaattatgag cagttttatc agcaggaaat gcatgtacgg
     3601 catcgtggag gctatcctcc ttattacttt actgtgaaaa taaccgccag ccatcctgaa
     3661 gaacaagtag cggcaaagaa aatctttcag attgccaatc agctaaaaga agtgttaagc
     3721 cctcaaagct tgctattagg gccaacgccc agcatgattt tgagagtgaa aaaccgatat
     3781 tattatcaac taatcatcaa atataaacat gaaccgaatc ttcctcatgt tttggatgaa
     3841 attttgaatg gaagccagaa agaacagcga caaggtcttt ttgtggcaat tgacaacgaa
     3901 ccattaaact ttatttaaga aagggccaaa ttatgcgcta tcctatattg atccacccta
     3961 atgataaatt aaaaagaact gctcagccaa tcgatgtaat cacggacgag acaatcgctt
     4021 tattagacaa tttatatgag acaatgattg cgaatgacgg aatagggatc gctgctcctc
     4081 aagtaggaca aaacaagaga attgctgtga tcgaagtaga tgaaggcgag aaatttgaac
     4141 tgattaaccc agaaatcatc gaagcaaaag gtgaaagcct tgacgtagaa ggttgtctca
     4201 gtattccgca tgtctatgga acggtcaagc gagcagatga agtgaccgtg cgttattatg
     4261 atcgagacgg agaagagata gaagtgacgg cttttggcta tttagcccgt gccttccagc
     4321 atgaaatcga tcacttagac ggtatcttgt tcattgagaa aatgatacag cagatcccgg
     4381 aagaagaact tgaagaatat atggaggaac atcttgatga ctaagctagt atttatgggc
     4441 acacctgctt tttctgtgcc gattcttgaa gggctgatag aatcaggtta cgagatccag
     4501 gcagtggtaa cacagcccga ccgtccagtt gggcggaaac gagtaattac gccgacaccg
     4561 gtaaaagaag cggctctgaa gcacggtatc cgtgtgctgc agccagagaa aatctccggt
     4621 tcgccagaaa tggaagaaat cattgaattg gtgcctgatg tgatcgtcac tgcggcattt
     4681 ggccaattct tgcctgaaaa gttattgcaa gtaccaaaat taggggcaat caatgtccat
     4741 gcttctttac ttcctaaata tcggggaggt gcgcctgttc attatgccat catgaatggg
     4801 gagaaagaaa ctggtgtaac gatcatggaa atgatcaaaa aaatggatgc aggcggaatc
     4861 tatgcgcaag aaagcatacc aatcactaaa caagatgatg tagggacgat gtttgaaaag
     4921 cttagccttt taggaagaaa gctattgcta gaaacattgc ccaatatttt ggatggacaa
     4981 aaaccagtac ctcaagacga gtcagaagca actttttcgc caaacatcac acgggaacaa
     5041 gaagcaatcg attggcataa aactgcagaa gaaattgaca accaagtacg cggaatgcgt
     5101 ccttggccaa ttgcattcac gacctatgag ggcatacgct ggaaattatt atcggttgaa
     5161 gtattggcag aaactacaga caaagcacct ggtacgatca tccggaaaga caaaaaaaat
     5221 ctatggattg cttgcggtaa acagaccgtc ttagccatca atgaattaca gccagctggt
     5281 aaaggaaaac aggccgtaca tgatttcctt aacggaagcg gtcagcaagt tcagattggt
     5341 cagcaggtga aataatgaaa aaaatcccta aaaaaatgaa aaaatcagta cgatatatgg
     5401 cattactgac attagagcga gtgaacaagg gtggcgccta ctcgaatctt ttgttgaatg
     5461 agatgatcaa acaaagcgag ctaaatcaaa aagatgtggg tttatttaca gaattgatct
     5521 atggtacgat tagccatcag cgactattgg aattttacgt tgcaccattg atcgctaaag
     5581 caaaaaaagt ggatgactgg gtcaagacgc ttctttatct ttctgtatac caattagaat
     5641 ttttagataa agtccctgca catgctattt taaacgaggc ggtcgaaatc gcaaaagtaa
     5701 aaggaaatcc tgggacaggg aaatttgtaa atggtgtctt gaggaattat cagcgtcaag
     5761 gagcaccaga tctgcaaggc attgctgatc ctatcgaaag actttctgtc aaaatcagcc
     5821 tgcctctatg gatgacgaaa cgactggtgg atcaaatcgg atacgaagaa actgaaaagc
     5881 tcggtttatc gctatatcag cctagccatg ctagtgcccg agtagataca cgccgacttt
     5941 ccagagatca ggccatcact gttttgcaag aagaagagat cacagctgaa aaaagccaag
     6001 tcagtccata cggtatagta gcgaaaaaag ggcatttagc ttctagctca ttgttccacg
     6061 atggcgtcat gacgatccaa gatgaaagtt cgatgcttgt agcgccgtct atgcaaatcg
     6121 agcctgaaca tcatgtctta gatgcctgtg cagcacccgg aggtaagacg acgcatattg
     6181 cttctttttt agatccgtca tgcggtggag aagtggtcgc tttagatgta cacgaacaca
     6241 aagtcaaact cattgaagat aatgcagcac gtctaggtgt ctcttctgct gtctacgctc
     6301 aaaagatgga tgctagagaa gtcagtaaac agttcgaagc agagcagttt gatcggattt
     6361 tagtagacgc gccttgttca ggtcttgggc tgcttcggcg aaaaccagat attcgttata
     6421 aaaaacagcc ggatgaatta gcgaatttac caaaaatcca attggaaatt ttagaaagct
     6481 gcgcaccaac tttaaagtct tccggtattc tgacttatag cacttgtacg atcttaaaag
     6541 aagaaaacca agacgttgtt tctgctttct tggaaagaca ccctgatttt gagaggattg
     6601 atgttttgac caatcctatg ctacaatcat caatcgaaga gaaaatgttg actttgtatc
     6661 cgcatcagtt ttacacagat ggatttttca tttgttgttt gcgcaaaaag taatgaggtg
     6721 aaaaaatgca gattgaatat caatcagatg taggaagacg acgtaacaca aatcaagact
     6781 atgccagtgt atttaccagc cgttcaggtc tcaagcttgc tgtattagca gacgggatgg
     6841 gtggtcatcg tgccggagac attgccagtc agatggcggt aacgaatcta ggcgaagctt
     6901 gggaaaaaca agaagctgtt gacgatgaaa aaatcgccca atggttcatt caatcgattc
     6961 aagaagaaaa tatgctgatt tatcagcgtg gacaagaaca gccggaatat aacggtatgg
     7021 gaacaacaat cgtagcggcc gctctgtcag atgactggtt cacgattgcc aatgttgggg
     7081 atagcagagc ttatttgatt cgtgaagata aaatcatgca attgacggag gatcattctt
     7141 tagtcaatga actagtcaaa tcaggagaaa tcagcgaaga aatggcagtg aaccatccaa
     7201 ggaaaaatat tttaacacgt tctgtcggca tgcctggaac agtcgaagta gatgtttcta
     7261 ctcatctttg gcaactaaaa gatcgactgc tgttatgttc agatggcttg acgaacatgc
     7321 tgtctgaaga agaaatagga acgatcatca atcaagaagg cccattgtct cataaagtaa
     7381 ctgagctgat tgatcgtgcc aatgaagcag gcggagcgga taatatcacc gttcttctta
     7441 tcgaatataa ggaggacagt gtatgatcga gttgggcaaa aaattaaatg gccgttatca
     7501 aatcactgga aatatcggta gcggtggtat ggcaaatgta tttttggcgc acgatttaat
     7561 cttggatcgt gatgtagcag ttaaagtctt acgatttgac tttcaaaatg accagactgc
     7621 gattcgacga tttcagagag aagctttagc agcaacagaa ctcgttcatc caaacattgt
     7681 tagtgtttac gatgttggtg aagaagacaa catgcagtat ctggtgatgg aatacgtcaa
     7741 aggaatggac ttgaaacgat atatccaaac acattatcct gttccttatg aaacagcagt
     7801 aaatattatg cagcagatcc tttctgctat ttctttagct cataaccatc aaatcatcca
     7861 ccgagatttg aaaccccaaa acgtactgat cgataatgaa ggtgttgtaa aaatcacgga
     7921 tttcgggatt gctatcgctt tatctgaaac atcgatcacg cagacgaata cgatgctggg
     7981 atctgttcat tatctgtccc ctgagcaggc cagaggaagt atggcaacga agcagtcaga
     8041 tatttatgct ttagggatca tcctttatga gatgttgacg ggtagcgtac cgtttgatgg
     8101 tgaatctgct gtaacaattg ctttaaaaca ttttcaagat gatcttccat ctataaaagc
     8161 acttgatcca aatgtgccac aagcactgga aaacgtgatt ttacgggcaa cggctaaaga
     8221 acctgcagac cgttataagt cagccgagga aatgtctgat gatctttcta cagtattatc
     8281 accagctcgt gcaaacgaag aaaaatggca gcctcatgtg atggataatg aaacaaaagt
     8341 tatcacaccc cttacggaag atgcaccaat gcctgattca ttcaaatcta tgcctctgcc
     8401 aaaagacaag ccagaaaacg aaaccttgcc agaggaggaa gaagttctgc ctgatcctaa
     8461 aaagaaagga cagaaaaaat ggtggctgat cctattagct attcttgctg ttttaggaat
     8521 cggaacagct gtattctttg cttctggcgg gcgaggagaa gttgttgttc ctgatgtttc
     8581 tgatctttct gaagcttctg ctagagaaac actcgcaaaa gcgaaattag aagtagcaac
     8641 aaaaacagaa gaaattgcag atgacaaaat cgaagaaggt aatgtagtga agacagaccc
     8701 ggcagcggga acgacagtca agcaaaaacg cgaagtcact ctgtatatta gtagcggcac
     8761 gaaaaaaatc aaattagatg attacagtgg taaaagttat gaagatgccg aagcaagttt
     8821 gaaagaatta gggttttctg ctgacttgat tcaaaaagaa gaggaattca gttcagaggt
     8881 agaaagcgga atgatcattt cgcaatctcc atcagaagat acagaagtcg atcctaaaac
     8941 agacacgatt actttcacag tcagcaaagg accagaaaca gtgactttga gtagtcatgt
     9001 aggagaaaac tacgaaactg ttcgtaattg gcttgttaca caaggattca atatcaataa
     9061 tattagtgct acttatgact atagcgacac tgtaccatca ggttcgatca tcagtcagga
     9121 tccagcctcg ggtgaagttg tagcagaaga aacttacatc aattttgtcg taagccaagg
     9181 aacagagcct aaattgaatg atatctccgg ctatacaaaa agcgaagcac aaagttattt
     9241 agctagtgtc ggtgcagaat atatcgggca tgagacttcc gaatattcaa acacggtgga
     9301 taaagataaa gtgattcgta cagtaccagg ggcaggaacg acaattacca aaggaatggt
     9361 tgtgaacgta atttactcta aaggaccaga tccagcgtct tctagttctg aaaaagactc
     9421 ttcagacagt tctagcagct caagtagctc cagtagcaca agcaactctt caaaagaaga
     9481 atcttccagc tccacaagtt catcagctag cacctcagaa agttcaaaat aagtgctgaa
     9541 taaaatatat cagaagggaa tgcgacattg atttgttgca ttctcttttt tgtgtttcta
     9601 tctcttttga tcaattttca agccatatac tttgagaagt ttgcatggaa taacacctac
     9661 tttcctcttc gtatggtaaa ataaacaaaa acggaggtgg cacttatcaa aggacaaatc
     9721 agaaaagcaa tcagcggttt ttactatgta tacgcaaacg gagagaccta tcaaacaaga
     9781 ggaagaggaa atttccggaa tcggaaaatc acgccgcttg tcggagatca ggtgatcttt
     9841 gagagtgaaa atcaaacaga cggctatctt ctggaaatct tgccgagaaa aaatcaatta
     9901 gttcgcccac cagtcacaaa tgttgatctt ggaatcattg tcacaagtat cgtagagcct
     9961 gcattttctt ataatctact tgaccgcttt ctagttacct tggaatacga gtcgatcgaa
    10021 ccgattattt ttttgacaaa gaccgacttg tcagacgacc cagaaaaaat cgaagagatc
    10081 aagcaaacct atactaaaat aggctatccg gtgattgtac ccaatcatac gggtgatacg
    10141 acagaactga tccgttactt cccagaacgg ctatcggtct ttatgggtca gtctggagca
    10201 ggaaaatcga cgctactgaa tcaaatatca ccggaactga atttagcaac aggagagatt
    10261 tctgatgcac ttggaagagg acggcataca acacgtcatg tggaactgat tcctatctat
    10321 ggcgggttag tagcagatac accaggattt agctcgattg actttttgac aattgaagca
    10381 ggagaactgc cgagacaatt tcctgaattt gtagaagctt ctgtccattg caaatttaga
    10441 gagtgcatgc atgctaaaga acctggctgc gaagtaaaac atagagtaga aacaggtggt
    10501 atcgcacaga cacgttacga caattatttg caattcttac aagaaatcga aaaaagaaaa
    10561 ccaatgtatg caaaaaaaga aggtaaacga aaataggagg aaacaagaat gaagatagca
    10621 ccatccattt tgagtgcaga ttttgccaat ttacaaagag acattgagtt agtggaaaaa
    10681 ggaggcgcag attacatcca cgtagatgta atggatggcc aatttgtccc aaatatcaca
    10741 ttagggccaa atattgttca ggcaatccgt cctgttacta aattaccgtt agacgtccat
    10801 ctgatgatcg ttgatccaga aaactacatt ccagcatttg caaaagctgg agcagatatc
    10861 attactgtcc acgtcgaagc aactcctcat attcatcggg cacttcaaat gacaaaaagc
    10921 ttaggagtta aagctggagt agttatcaat ccaggaacac cagtctccat gatcaaacat
    10981 gttttgcctt tggctgatca agtactagtc atgactgtca atcctgggtt cggcggacaa
    11041 agctttattg aagaaacagt agaaaaaatt acggaactcg ctgatctacg tagagagaac
    11101 aattggactt atgcaatcga agtagatggc ggaatcgttc cagaaacagc aaaaatctgt
    11161 aaagatgcag gggcggatgt cttcgttgca ggatcatata tctatggagc cgaaaatcca
    11221 gtcggacaaa tcgaacgatt aaaagaggca ttagactaaa tgaaaatttt attagctgct
    11281 ggaggtaatc cgcaaaactg gccgcagtct ctgctgtcca aatacgatgc ctatgtcggg
    11341 atagaccgtg gtgcgctgta cctgcttcaa gcaggctacc cagtagatct ggcagtgggc
    11401 gattttgatt cattgtcgaa cgaagagcag aaggaagtat ttgcaaaagc caaagagtat
    11461 acccgttccc ctgcagaaaa agacgataca gatacacagt taggtatctt aaaaacattt
    11521 gaacgttacc cgcaagcgga agtcactttg attggagcta cgggcggaag attggatcat
    11581 cttttagcca atctctggtt aggtatggaa cctcgcttca aaccctttgc ccatcagctt
    11641 attatcagag attgtcaaaa ttatttgtct tattatttgc ctggagagca tatagtaaga
    11701 cagataccag atatgaagta tttagcttat tgctgcttga ctcccgtctc gaatcttaca
    11761 ttaaaaggca gcaagtatgt attggaaaat aaagaagtag aatatccgac atcttatgca
    11821 agtaacgaat ttctttcaga taccgcagaa ttttcttttt cagatggtat gattgcagtt
    11881 attcaaagca aagattgatc cataaacaaa taaaaaaatt tgtctttcag gctaaaaatg
    11941 acagaatcta ttatttgcta aaaaaaagct taagaggagc aacttacctt ttgtttcacg
    12001 gcatttagtg ataaaattga acacaagagt ctaattaatt cgaggtgtct ttgtgaagaa
    12061 gttcaatcct aataaaaata ttattattac cttggttatt gttatcatcg tggtgacgat
    12121 actaagtgtg accgctgcgc agcgagcgaa tgaaggaaag cccaatttct tccaatccat
    12181 cgttaatgac agtgttgcaa tggttgataa aacaatctct gccccagtac ggtgggtacg
    12241 aaatggagta gattctgtcc ataatctttt ttcaacttat gccgaaaatg agcggctaaa
    12301 agaaaaaatc gatagctatg atgacgtttc acagcaaaac aagaattacg agagagaaat
    12361 cgaagcgttg aaaaaagaac ttgagttgaa tcagacgctg actagttacg aaaaagtaac
    12421 ggcaaatgtc attactcgtt cgcctgacag ttggcaggac atgctgatcg tagacaaggg
    12481 ttctaaagac ggtatcgaag caaatatggc agtcatgtcg caaaaaggat tgattggccg
    12541 agtgatcgag gtcaatacgg cttcgtctaa aatcgaatta ctgtcatcct ctaatgaaag
    12601 ctccaatcat tttccagtac gggtatcttc ggctaatggc gaagcgtttg gtttgcttaa
    12661 aaactatgat gaaaagctcc atgccttagt ggtgacccaa ttaactggtg atacggatat
    12721 caaagaaggg gatgttgtcc agacatccgg tcttggaggg aattctccag ctaacttgcc
    12781 gatcggtacg gttattaaaa cgaaaccaga tagttatggg ctggatcggg aagtttatgt
    12841 gaaaccttat gcagaaatgt atgacgtgtc agttgtgacg attgtccaac ggctagcgga
    12901 ggattagtcc atgttgaaga aagaaacaat gaagtactat ctgccaatcg ttttgttctt
    12961 tttgatgttg atagatggtc atttaacaag aatgctaggg gagtggtcga aaggcaccta
    13021 tatgtcaaat gcccactttc tgatattggc attattatgt tgcagtatgg cgtttgaaaa
    13081 acgttattta ctgattacca cgattgttct cggggctatc tatgacgctt actatattgg
    13141 cgttatcggt atctatgcag tagctctccc tttaattgta tggttgatgt atgtaatgaa
    13201 agacgttatc catgtcaaca tctttactga atttttcagt atgatcatct ttgtcacggg
    13261 ttatgaattg tttacgatgg tggtccagtt gatttttaaa ttagcagtag taaataacac
    13321 gtattttatt acaaggtttt taggacctac actgctgttg aacatgatta tatttgtatt
    13381 attcattttt ccctttaaga aattattcag tgatgaataa tttctttttt tatttttagg
    13441 tcgaaagcga aaaagaatcg ttgttatctt aagaaatgct agacttaacg cggctttttc
    13501 atgatcattt tctgaaacga acagttttgt catttttaaa agttgttttg aaacattttt
    13561 gtaacataac tattatatcg ttgacacact gctatggtac aatttttata tcgttaagaa
    13621 agacttaaat atatttagat atttattaaa ttaagccagt cggaggaatg aagagtgaaa
    13681 aagagtttaa tatcagcagt aatggtatgt tcaatgacct tgactgccgt agcatcaccg
    13741 atcgctgccg cggcggacga ttttgattct cagatacaac agcaagatca aaagattgca
    13801 gacttgaaaa atcaacaagc agatgcacaa tcacaaatcg atgccttaga aagccaagtt
    13861 tctgaaatca acacacaagc acaagaccta ttagcaaaac aagatacatt gcgtcaagaa
    13921 tctgcacaat tagtaaaaga cattgcagat ttacaagaac gtattgaaaa acgtgaagat
    13981 acaatccaaa aacaagcacg cgaagcacaa gtaagcaaca caagctcaaa ctacatcgat
    14041 gctgtattga atgctgattc attagcagat gctattggac gcgttcaagc aatgacaacg
    14101 atggtaaaag caaacaacga cttgatggaa caacaaaaac aagataaaaa agctgtagaa
    14161 gacaaaaaag ctgaaaacga tgctaaacta aaagaattag ctgaaaacca agctgctttg
    14221 gaatctcaaa aaggcgatct tttatctaaa caagctgatt tgaacgtact gaaaacttca
    14281 ttagctgctg aacaagcaac tgctgaagac aagaaagctg acttgaaccg tcaaaaagca
    14341 gaagctgaag cagagcaagc tcgtattcgt gaacaacaac gtctagcaga acaagctcgt
    14401 caacaagctg cacaagaaaa agctgaaaaa gaagcacgcg aacaagcaga ggctgaagca
    14461 caagctactc aagcttcatc tactgcacaa agctcagcaa cagaagaatc atcagctaca
    14521 caatcttcaa tgactgaaga atcatcatca gctacacaat cttcagcaac agaagaatca
    14581 acaacacctg aatcttcaac agaagaatca acagcgcctg aatcttc
//
LOCUS       DACDYJ010000063        14211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDYJ010000063 DACDYJ010000000
VERSION     DACDYJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.063"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="IWM51_002076"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="IWM51_002076"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP9971479.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="IWM51_002077"
     CDS             complement(2262..2513)
                     /locus_tag="IWM51_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP9971480.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="IWM51_002078"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="IWM51_002078"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP9971481.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="IWM51_002079"
     CDS             3463..3681
                     /locus_tag="IWM51_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971482.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3970..4482)
                     /locus_tag="IWM51_002080"
     CDS             complement(3970..4482)
                     /locus_tag="IWM51_002080"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP9971483.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="IWM51_002081"
     CDS             complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="IWM51_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP9971484.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6935..7369)
                     /locus_tag="IWM51_002082"
     CDS             complement(6935..7369)
                     /locus_tag="IWM51_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP9971485.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7379..8167)
                     /locus_tag="IWM51_002083"
     CDS             complement(7379..8167)
                     /locus_tag="IWM51_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9971486.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8164..9105)
                     /locus_tag="IWM51_002084"
     CDS             complement(8164..9105)
                     /locus_tag="IWM51_002084"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP9971487.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9282..9869)
                     /locus_tag="IWM51_002085"
     CDS             complement(9282..9869)
                     /locus_tag="IWM51_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP9971488.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="IWM51_002086"
     CDS             complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="IWM51_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP9971489.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11699..12709
                     /gene="gap"
                     /locus_tag="IWM51_002087"
     CDS             11699..12709
                     /gene="gap"
                     /locus_tag="IWM51_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9971490.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12794..13729)
                     /locus_tag="IWM51_002088"
     CDS             complement(12794..13729)
                     /locus_tag="IWM51_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9971491.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13915..>14211
                     /locus_tag="IWM51_002089"
     CDS             13915..>14211
                     /locus_tag="IWM51_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9971492.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKER"
BASE COUNT         3968 a         3160 c         2510 g         4573 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat
     3901 ctgacttttg agggtcgctt taaaatcgct tctttaagaa taacatcaag aatagcttct
     3961 tttttctgct taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc
     4021 atggagatcc atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc
     4081 gatcagttcg atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag
     4141 cacacgttga cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc
     4201 taatccataa gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc
     4261 aaaaccgact cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg
     4321 cccaactacc atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc
     4381 ccgataagcg tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga
     4441 cggatagtcg ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg
     4501 ttgttaacca tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga
     4561 ttttattgag ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag
     4621 cgactttttg taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta
     4681 tcataaaaat taataatttt tcagggatac gcaaatattg agctattgct tttattttat
     4741 gacgtatatc aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc
     4801 ttgtgcctac cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat
     4861 tgaatgaata agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa
     4921 caactagaga gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga
     4981 aaacaatcgc agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg
     5041 ctaaataaag gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat
     5101 caaatacttg cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc
     5161 cgttccattc attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt
     5221 cttcttgagt accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga
     5281 caaatttcag atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc
     5341 gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat
     5401 caattcgctc tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc
     5461 ctctcatcaa gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag
     5521 tgacattttc acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa
     5581 ataaatctcg catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag
     5641 atcctttagc tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat
     5701 tcattatttt cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata
     5761 gatggatatg attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga
     5821 ttgcttttct ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt
     5881 catcaaacgt cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat
     5941 ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc
     6001 cgctttcttc aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc
     6061 ctaatgaaac aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta
     6121 tcgctactaa atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga
     6181 atgctactcc gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt
     6241 gaacaatcgc ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca
     6301 cgtctacacc agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa
     6361 cagtgatgat caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc
     6421 catacccatg ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca
     6481 gtgtttcttt cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga
     6541 ggattttttc tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt
     6601 gtatcagata cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct
     6661 tttcctcatg gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt
     6721 cacggatcat ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat
     6781 ttgcttgtct cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa
     6841 aaagcagaaa gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct
     6901 gatcttttgt acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt
     6961 tgggcgattg agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg
     7021 cttgtaactc cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt
     7081 gctgttcttc ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt
     7141 cctgagtcag ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa
     7201 atacaatcag tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa
     7261 aggaagtgaa gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa
     7321 taatcaaaac taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct
     7381 actttttgtt aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa
     7441 attttgatgc aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat
     7501 tgcggacgat catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca
     7561 aatagcttcc gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca
     7621 ctgtcgtaac agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa
     7681 ggactgcaaa tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca
     7741 tagaggcgat attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt
     7801 gtgtcaaaac catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt
     7861 cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc
     7921 gcccgacttc ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt
     7981 ctaccttgaa agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt
     8041 ctacaaatgc cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat
     8101 aacagatttg tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta
     8161 aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat
     8221 cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa
     8281 atatctcgca ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc
     8341 agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt
     8401 aaatgtgctt tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt
     8461 ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc
     8521 ttttccattg atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc
     8581 gtacagtggt ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt
     8641 atgatcatgt tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt
     8701 ataaacagaa aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt
     8761 cagcggataa cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat
     8821 cccttttggc ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat
     8881 gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat
     8941 ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa
     9001 caaccaaact tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg
     9061 ttttgcagga acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt
     9121 ctttaagttc ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa
     9181 tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa
     9241 aaacagcagc gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa
     9301 ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct
     9361 ttcgcatgat tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc
     9421 aagcgagtaa tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc
     9481 tcttcatcag caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag
     9541 tcgaacacgg tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct
     9601 ccggcaaacc atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg
     9661 attaatccac ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg
     9721 aattgatgtg tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa
     9781 cgaacagcat gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga
     9841 aaatttaaaa tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat
     9901 ttattttacc tttaattgta aaataagaaa agaaaagcac tctttttttc caataaaata
     9961 gggctgtgac aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct
    10021 gatcacttct gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta
    10081 tttgcttgcc tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc
    10141 tttcgcaccg cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa
    10201 acgcatcact gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc
    10261 accagataag atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg
    10321 attgaatcca tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg
    10381 cgttacttct aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac
    10441 tccagaaatt ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag
    10501 ctgttctttg aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac
    10561 gatgatttgc ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc
    10621 aagatagtct tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat
    10681 tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc
    10741 tccttcaatc aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac
    10801 cattcctaga ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga
    10861 tgaaataacg gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc
    10921 tgccaatact ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc
    10981 aatttccggt gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc
    11041 accttttgcc acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt
    11101 ctccgcatct cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg
    11161 accgtgcatc cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa
    11221 ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg
    11281 accaccgtct ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac
    11341 cattccgtct ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga
    11401 cataattaag tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa
    11461 caaaagtctt acttatttta tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga
    11521 ttgtttacct tcttcttttt ttatcatttg gtaaggtttc caaccttttt tctgctacca
    11581 agtataattt ttgacaaaat attctttgcg tagcagaata acagtgtatt aattgtatta
    11641 attgtattaa ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat
    11701 gacagtaaaa gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat
    11761 caaagaagtc tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat
    11821 gttagcacaa ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc
    11881 aacagaagac tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc
    11941 aagcaaaatc ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt
    12001 ttatacatct gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat
    12061 ttctgcccca gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga
    12121 tgaaaacgac aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc
    12181 ttatttctta aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta
    12241 cacttccaca caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg
    12301 ttcagctgct gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt
    12361 tatccctgag ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg
    12421 ttcattaact gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga
    12481 agcaatcaaa aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt
    12541 ttctagcgat gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt
    12601 aacaactgct ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg
    12661 atttacttgc caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc
    12721 aatcgataaa agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta
    12781 cttttaaact cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt
    12841 tttcaggatc tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt
    12901 ttggtccctt cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt
    12961 cgtctccatg tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg
    13021 cttcaccgaa aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg
    13081 gaaatgctgg aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag
    13141 aataaccgca atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca
    13201 tcataactgt tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat
    13261 tgttttctag cactttgttt atccactgta catagtcttt acgctctggc catccaaatc
    13321 caatgtaatc cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac
    13381 ccatatcatg aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat
    13441 gagccacaag agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt
    13501 tcagaccgtc ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt
    13561 tatgtgcatc cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt
    13621 ctttctcaga aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa
    13681 caatcagagc taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat
    13741 ctcatttctc ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca
    13801 atgttcattt ttataacccg cgaattgtaa aattaagtta gaaaaataaa aaggcattta
    13861 tggtacactc aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc
    13921 tacaaacatc ttaccataga cgaactgaca atgatagaat catattatct tcaacataat
    13981 aagccggttg aaatcgctaa ccgaatggga cgtgctatac aaactattta taatgtagtc
    14041 aataagttca agcaaggcaa gactgctctt gattattggc accagtataa agaaaataag
    14101 aaaaaatgtg gtagaaaagt cattcaatta cctgctcatg aagtagatta cattaaagag
    14161 aaagtcactc ttggttggac gcctgacgtc attatcgggc gaaaagaaag g
//
LOCUS       DACDYJ010000064        13988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDYJ010000064 DACDYJ010000000
VERSION     DACDYJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.064"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(120..776)
                     /locus_tag="IWM51_002090"
     CDS             complement(120..776)
                     /locus_tag="IWM51_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9971493.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            969..1247
                     /locus_tag="IWM51_002091"
     CDS             969..1247
                     /locus_tag="IWM51_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971494.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1266..2474
                     /locus_tag="IWM51_002092"
     CDS             1266..2474
                     /locus_tag="IWM51_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9971495.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2622..2987)
                     /locus_tag="IWM51_002093"
     CDS             complement(2622..2987)
                     /locus_tag="IWM51_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP9971496.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3134..3907)
                     /locus_tag="IWM51_002094"
     CDS             complement(3134..3907)
                     /locus_tag="IWM51_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9971497.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4081..4941)
                     /locus_tag="IWM51_002095"
     CDS             complement(4081..4941)
                     /locus_tag="IWM51_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP9971498.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4941..6359)
                     /locus_tag="IWM51_002096"
     CDS             complement(4941..6359)
                     /locus_tag="IWM51_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9971499.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6416..6865)
                     /locus_tag="IWM51_002097"
     CDS             complement(6416..6865)
                     /locus_tag="IWM51_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9971500.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7310..7573)
                     /locus_tag="IWM51_002098"
     CDS             complement(7310..7573)
                     /locus_tag="IWM51_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP9971501.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7672..8967)
                     /locus_tag="IWM51_002099"
     CDS             complement(7672..8967)
                     /locus_tag="IWM51_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP9971502.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8964..10235)
                     /locus_tag="IWM51_002100"
     CDS             complement(8964..10235)
                     /locus_tag="IWM51_002100"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9971503.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10400..11269)
                     /locus_tag="IWM51_002101"
     CDS             complement(10400..11269)
                     /locus_tag="IWM51_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP9971504.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11561..13171)
                     /locus_tag="IWM51_002102"
     CDS             complement(11561..13171)
                     /locus_tag="IWM51_002102"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP9971505.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         3982 a         2947 c         2386 g         4673 t
ORIGIN      
        1 gtgttcaatc agctgaccct cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
       61 tttccgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct ctttgttcat
      121 caaaagataa tattgtcttc taacaagtcc atattcaaaa taacgatatg atggtcttta
      181 acggtgataa taccatcttt tttcagttgt tttaaaattc tgtttacact gctagcagtt
      241 gagatgccac aaaatttggc gatttcttca ttcgtgatcg aaaaatcgat cagcatccct
      301 tcttctgtcc gcacaccgaa taaatcatat aattcgtaaa gttgcgtaca aacagctccc
      361 agctttccgt ttaatagcat ttgctgcatt tttttcatcg aataaagcag acgatagcgg
      421 taatactctt ttacatagtg ttgtagcttt gggtcttggt tgatattttg ccaaaagcgc
      481 acacgattga ttcgataaaa ctctgcatat tctgattcaa ctcgaatatt atacggcgaa
      541 tcgatgaatt gtgaatattc gtcttttaaa agagagacga tttcaagatc tttgatatag
      601 cgaaggttaa attctctccc atcatttgaa ataacacttg cttttaccgt tccctctttc
      661 aagatatacg tatgggaatc ttccatccct tcgaatgtca aatatttatg atatcctttg
      721 cgtataatcg gaaaatcatt tttatccaaa tattccttta agactctact atccaatgat
      781 gctcccccat ccaaactttc atctaaaaag atcatacagt actttaaaac tttttaaagt
      841 ccaaaggtat tttactaatg atattaacat ttgttaagta cctttttcca tcacttatgc
      901 gatttttttc ctttttttta ttcgttatac tgcagaagta aaatttttta agaatagagg
      961 gaaaaaacat gaattactgg atgaacacaa tcattaatcg attagaaaca gcatatcaaa
     1021 caagatttga tatgaaagct tctttagtct ttttaaatga tgcgtatcaa aactcgatcg
     1081 aattgatcaa agcagtggat gagaatccaa caaacgaatg cgaagaattt ttaaatctgt
     1141 ttatgtctac aagagattta tttattcgcc aacttgttga tcgctatcca tccaattatc
     1201 acgatgtaga agttcaaata caaaaattaa aagcatattc agcttaaggg gaatgtataa
     1261 catacatgga aaagaaatta tttaataaat attttattgg aatcacattg atcaatttta
     1321 ttgtctacct gatttattat ctgttgatgg tcatcatggc agtcattgcg caagacacct
     1381 tacacgccag cttgagccaa gcaggttttg cttctgggat ctatatcatc ggaacattgc
     1441 tggcacgctt attcatgggg aaacagttag aactatttgg tcggaagttt accttaagag
     1501 gcggcgcgat cttctatctc ctttcaacat tagcatatct ttatacgcca actatcgggg
     1561 cattgtattt agttcgattc ttgaatggat tcggctacgg tacagtttct acagctacaa
     1621 atgctatcgt tactgcatat atccctgcat ctaaaaaagg ggaaggcatc aattattatg
     1681 gattaagtac tagtttagct gccgctgttg ggccattttt aggaatgatt ttgctgaata
     1741 tgactgattt tcgtttcatt atctggtttt caattgtact ggtcttcttt gttacgattg
     1801 cctgccttgt ctttcctgta aaaaatattc cattatctga agaacaaaaa atatctcttc
     1861 gctcatggaa tatcaatagt tttattgaaa agaaagcact tttcattaca ggaattgcct
     1921 ttttgatggg tctttcttat tcaagtgttt tatcattttt atcttcttat acaaaagtaa
     1981 tccacttagt tgctgctggg tcatttttct tcgtcgtata tgctttggtc atcacattta
     2041 cacgtccatt gactggaagg attttcgatg tcaaaggaga acaatacgtg atgtatccta
     2101 gctatttatt cttaacagca ggattatttt tactgagtgt aacgacgaat agtgtgacat
     2161 tgcttatctc tggagcatta gtcggcctag gttatggtac gtttatgtca aatggacagg
     2221 ctgtttgttt aaaaatcgtc cgcgaacatc gaatcagtat tgctttatct acctacttta
     2281 ttggtttaga cctaggctta ggcgttggtc cttatttgat ggggatgcta aaatctctta
     2341 cttcatttag aggattgtat gtgattgccg gagtcattcc attgatatgt acagcactct
     2401 atcttgttaa tggccgaaaa catagtcatg aagcagaggg cgaattgatt gaatcattga
     2461 aaaatgaaat ttgatcttga tgaatagata aaaaaaaccg ttcagatctg aaaataatct
     2521 gagcggtttt ttgagtaaaa atagtttatg attattctta attaaacatc tatttatttt
     2581 taaagctttc atttatcgat aagaatagtt taactgttga tttattccat tttccgttca
     2641 tctggtacga tgccttgact tagcaaccac ttgtggaaga accattctgc cacaccgatc
     2701 aatagagcag gaatcaatac tttcatccaa atatcgactg tatcattcat aaaatataga
     2761 tagagccaca agataactgc agctgaacca gcatctgcga tcgtcgctgc gatgttccct
     2821 actttacgta agatcaacag atcccctagc acatagatca tgactacaat aattacccca
     2881 acaatcgttg aatccataaa gctaacaccg tataaacctg tcagcacgac ccacagaatc
     2941 gcccagatca tgattgcctt cactaccaac gccttcacat gtttcatttt ctgcactcct
     3001 ttctcctgga taaaaaagta agattatcta acaagataac cttttcatta ttataccatc
     3061 ggctgagaga agcgagcaaa gataaggtcc tatctaaaaa tgaggaagcc acttagaaca
     3121 ttcacgtaat aatttatccg tacagacatt cgatatggtg ttcctttact ttcgcatact
     3181 gttcttttgt cagttcttta cttgtcacca gataatcgat ttcatgaaaa gcaatggctt
     3241 gatgcatgaa tttatgtgca attttttgct gtgtaactaa aacggcaagc cggtctgatt
     3301 gttttctaaa agcttttttg accattgcct ctccttcatg agtttctgtt actccttggc
     3361 tgaaatctag cccgcgacaa ctcattatcg ctaaatccgc ataatatgac aatgcatcgt
     3421 tataggcttt tgaaccgttg attgttgctc gtttcggaac aattgcccct cccaatacag
     3481 atacttttgc cgttgtatat tcactcaaca tagcagctgt gacgatgcca ttcgtaatga
     3541 tctgcaaacc atcaaatctt gctaggtttc tgactaagtg taaagaggtt gtactggaat
     3601 ccaagaataa gaccatatca tctcgaatca aagaagaagc tgtatctgca atcttacgtt
     3661 tttctgtttc ttcttctaat ttggtgatct tcatcaggta aggcacgtca atttttcgat
     3721 tgtcaataag catggcgcca ccgcgaactc tacggatcaa tccacttttt tccagaaaaa
     3781 caaggtcacg tttaatcgtg gcttcgctat aatttactaa ctcgatcaaa tcggtgactc
     3841 ttgcaaattg gttgtttttt agataattca caatctcctg ctgtctattc tccacttcat
     3901 tcaacataaa atcaccccat ttactttata tagtaacagg aaaaaagagg ttgtgacaga
     3961 agtgattggc ttcgagaaat aagaagaaat ttttcgaaaa aactacttct gaacatcgtg
     4021 tgtggttaag agggtatgac aagactattg tcataccctc ttgaccacac atttttattt
     4081 ttagtataaa ctagccttgc ctactgtgtt caaaatttcc attttatgtt tgaccacttc
     4141 ttgtaccttt ttattagctg ctggataaat tgcattcggc tcgtacatcg atggattttc
     4201 actcaatatt tctctcaaag cattgaaaaa gacgctttta agatctgagc tcaaattcac
     4261 tttggcaact ccgtatttta ctgcttcgct gacttctgcg tccggattac ctgaaccgcc
     4321 atgtaaaaca aatggaagat cgatttgttg gttcaattct ttcagtaagt cgatatttaa
     4381 ttttggagtt ctgtttttcg gatacaagcc atgcgctgtt ccaattgcta cagcaagtgt
     4441 atcgattcct gtttctttca caaaaatctt cgcttgatca ggatctgtat aaatgattgt
     4501 ttcagcacct ccttcagcag aaccattatt cccaatcgtt ccaagctcag cctccactga
     4561 gacatttacc gcatgagcta acgcaaccac ttttttcgta atctgtacat tgtcgtcgaa
     4621 tgatttcaaa gacgcatcga tcatcacgga agaatagccg tttcgaatcg cccgtaagac
     4681 atcttctatc gtaccgccat gatccaagtg gataacgatc ggcagtttac ttgtataagc
     4741 ataggctctt actgccgcaa caaactcatc acttaaatag gcgatttcat caggatgaat
     4801 ttctaagatt actggtgctt ctaatctctc tgcttcttcg atgattgctt tcatcatatc
     4861 atagctgcaa atattgaagg ctggtactgc aaattgattc tctttagcca ctttcaataa
     4921 ctctttcatt gtatataaca ttacgcttcc acctcatttg ttagattttc atttttcgtc
     4981 cacttgtctt gttttttctc tttcactgtt tttttcaata gtccaagcat aaaggctgta
     5041 gtgactgtac ctgatgcaat tgccaagaca tacataggta aattgttcat aacatttgga
     5101 attagcatca cccaaatccc tccatgaggt gccattgaag taactcctac tgccatggaa
     5161 atccctgcag cgactgctga acctgtcatc aaactaggta tcacgcggat cgggtcagat
     5221 accgcaaacg gtatagcacc ttctgtaata tacgaagcgc ctaaaatcca gcatgattga
     5281 cctgaagtcc gttcttcttt ggtaaattta tgtttgaata ataaagtggc taaagcaata
     5341 cctaggggag gtgtcattcc tgcagccata caagcagcaa tcggtgcatt tactccagct
     5401 gacatcaaac cgatcgagaa agtcgaaatc gatttattga tcggcccacc catatcagct
     5461 gccatcatga tcccgataag taatccgaac aatagcccgg atgtatttcc caagctattc
     5521 agccaatttg tcaaagcatc caacacccat gtgatcggtt gttcaatcac aaataccatc
     5581 aaaagcccaa taattaagat cgataaaaag gggacgacaa tcagctgata aatagcagaa
     5641 aattcttttt ttacgctaat tttatttgcc agaaaatcac agatataccc tgctagcacg
     5701 ccaccaatca ttccgccaag aaaacctgaa ccacctgtag aggccagtaa tccggcaaca
     5761 attcctgggg caaaacctgc ttttccagca atcgatgtag caatccccgc gcctaacgca
     5821 ggaaccatca tggcaaacgc tgtatctcct ccgatccttg aaaatgcagc tgctaacaca
     5881 ttatagtctg ctgagttggg atcagccgct gtaatcccaa aggcaaaact cagtgcgatg
     5941 atgattccgc ctgcgatcac aaatggaagc atgtaattca caccattcat gaaatgatta
     6001 tagattgtcg gctttttctc atttgcagct ggttcttttt cattagacac aacgaatacg
     6061 ccaatatctc tcaatgcttc gtctattact acttgcggtt ctttgattgc tcggcttgta
     6121 gagacacgct ttactttttt cccggcaaat cggtccaatt cgatttcttt atctactgca
     6181 agaatgattg catctgcctc tttgatttct tgttcagata actgattttc tgtttttaca
     6241 ccttgtgttt cgaattttgc ttgatggttt agatttcttg ctgttacttc taatttttct
     6301 gccgacatat aagtatgagc aattccagtc gggcaagcag ttacaccaac gattttcatg
     6361 attattttcc tcctttttct tcactatcga tactcggaca aagactctac tattttcatt
     6421 cttgactaat ctgtctgatg atatctgctg gcgtagtggc ttcttttatt ccctttctaa
     6481 aatctgcatt catcaatcgt ctggagagtg tgctgagaat ctctagatgc atatctgctg
     6541 atttttcagg aactacgatg gcaaaaatat attcaattgg catttcatct atcccgctat
     6601 agtccgttaa tttggctgta tgaaaaaaac acagtgctgg ttttgtcaca tatttgcttt
     6661 ttgcatgggg aatcccaaat ccatcttcta ttcctgtgga gatctcctcc tctcgtttga
     6721 atagtgcctt aacgactttt tgaacatttc ttaccttatt tgtttttttc aacacatccc
     6781 ctattcgctc aaataatgat ccttttgaat ctatcgatgg atcgaaaaca atcagttctt
     6841 ctgcgatcaa ttgatcaaac ctcataaaaa acctctcccc cacaaaaaat gttaacgttt
     6901 tcttggaact gaggtcagta tagcatcgct agctgtaccg ttcaatatta tgaatgaaca
     6961 gaggaaaaag acccatcatc cgcgtgtata agtcattaaa atgaacgatc tagctcacaa
     7021 acaatcgcaa gtctataaca aaacaaaaag ccttccttga aaggaaagct tagcttctaa
     7081 ttttaggtaa atggtttttt acttcgaaat aacccctctt catttcgtta atataaggtg
     7141 tttttatcaa gtggataatt aaaactatat actagttttt cattgttttg tgcattatga
     7201 tcctacgact ttttcagcat caaacgaagt tttaaattgg caaaaaacat aaaaaaacag
     7261 actatcccta tcagatagtc tgctaaacgt tgagttatca gcgtttttat tattccgcag
     7321 cgtttgcgtc tttgagaccg tattttttgt tgaaacggtc cacacgtccg tctgcttgtg
     7381 tgaatttttg acgtccagtg tagaatggat gtgagtcaga agttacttcg acacggatga
     7441 ctgggtatgt attaccgtct tcccattcaa ctgtttcttg tgaagttttt gttgaacctg
     7501 acaagaattt gaaaccagtt gttgaatcca tgaatactac tgggtggtaa tctggatgga
     7561 tgttttcttt catgattttt tcgctccttt gccctgctcc atttgctgaa ccagagttga
     7621 tttgtcgatt tacaacatta acatcttaac atgtttcagt caaggttgca attatctttt
     7681 cagatttctg gtcggatttt ttttaccaaa ggagacatct tgaaattctt tgaaaaattg
     7741 ctgattgttt tttgttttac gcaaaaagcg gatcagttgt tccgtatatt ctaatgaatc
     7801 tccagtcata ttttttcgaa gtttccaaat ttcttctaat tcttcacttg gcatcaataa
     7861 ttcttctttt cgggtacctg attttttgat atcgatggct gggaagatcc gacgttcaga
     7921 taattcacgt gaaagttgta attctagatt tccagttcct ttgaattctt catagatcac
     7981 gtcatccata cggcttcctg tatcgactaa agcggtcgct aaaatcgtca aactgccacc
     8041 ttcttcgatg tttcttgctg ccccgaaaaa tttcttaggt ttatacaatg cggctgggtc
     8101 aatcccgcca ctcaatgtac gtccgcttgg tggaacgact aagttatagg cccgagcaag
     8161 tctagtaata ctatccatca agataacgac atctcgttta tcttcgacta aacgcattgc
     8221 gcgttccaat actaattcag aaactcgagt atggttttgc ggctgcagat caaatgtaga
     8281 agataccacg tcgcctttca cgcttcgttc tagatccgtt acttcttccg gtcgttcatc
     8341 aatcagtaga acgattaatt ctacttccgg atagttttcg gtgataccat ttgcgatttc
     8401 ttttaataca gaagttttcc ctgcttttgg cggtgcgacg atcaatccac gctgaccaaa
     8461 accgattgga gaaaagatat cgatcattcg ggtagatagt ctttgcggtg ttgtttccag
     8521 ttgaagctgt ctttgcggat aaagaggtgt taaggctgga aaatgcggac gttcttttgc
     8581 ttcttccgga tcttttccat taacgctttc cacgtgcatc aaaccataat aacgttccga
     8641 ttctttaggt ggacgggctt ttcctgccac tttgtcccca tttctcaaac tgaatcgtct
     8701 gatttgtgag gcagaaatat agatatcttc tgcacttggt ccataattga ttggccgtaa
     8761 aaaaccgtaa ccatcttgac caacgatatc caatattcct tccatataat aaaatccttg
     8821 tttctctgct tgtgcacgaa tcacagctag agataattcc tttttattca tcttactgta
     8881 ataaggaatt ttaaattctt tggcataact ataaacttct tttaacgttt tgttctctag
     8941 ttctgccatt gtcaaataat cgctcattca gctcccactt cctctatcat ttcaattgat
     9001 gcccctaagg ctgttagttt ctcgataata tgatcataac cgcgtaagat ataatctaca
     9061 ttgtaaatcg tcgttgtccc ttctgccaat agtccagcaa tcactaaaca agcacctgcc
     9121 cgaagatcag aagccacgac ttctgtgccg tgaagtttat ttggaccatt cacgatgatc
     9181 atatttcctt ccacggtaat atcagcgccc attcgtgcta gttccggcac atggttcaca
     9241 cgtttcgcat agatcgtatc gatgatttct gaagtgcctg tagtcatcaa tagtaacgca
     9301 gtaagtggct gctgcagatc tgtggcaaat cctggataag gataggtcat gatgtttgcg
     9361 ggttttaatt ctttcgaggg gtaaacttcg atttcatctt cgctgatttt catatggaca
     9421 ccaatttcct gcaattttgc tataaagctt tccaaatgtt caaagatcac atttttcact
     9481 ttgataccat ttcctactgc agctgctaat gctagatagg ttcctgcttc gatgcggtca
     9541 ggaatcatga aatgccgaca gccatgtaat gtttcgactc cttcgatacg gatcacatct
     9601 gtacccgctc ctcgaacttt tgcccccata ttattcagta aagttgccac atctatgatt
     9661 tccggttctc gtgcagcgtt ttcaattact gtctgtcctt ttgcttttac agcagctaac
     9721 atcacattga tcgttgctcc gactgaaacc acgtccatga aaatccgatt gccacgcaat
     9781 cctttatttt ctgtccgtaa atacatggca ccatgttcat ttgtcacttc agcacctaga
     9841 gcttcaaatc ctttgacgtg taggtcaatc ggtctagggc ctaaataaca gccacctggc
     9901 aggcctacta ccgcttcgcc aaatttacca agcagtgttc ccataaaata ataagaagca
     9961 cgcaaactat tgatttttcc gtgcggcatc ggaatcgata cgacttctcg aggatcgatt
    10021 tctagtatgt tgttggaaaa atggacagtt gcgcccatga tctctaaaat ttcaattaat
    10081 gaatgaacat cttgtatgtc cggcacacct tccaagatca ctggggaatc agctaagatt
    10141 gcagctggaa tcaatgcgac tgcactattt tttgcaccgc tgatcgttac ttcaccagct
    10201 aaaggtcggt ttcctttgat tacgattttt ttcatctgtt tccaccttgt ttattaatct
    10261 tttttcatgg atatgcttgt attttatcat agataaaaaa aagaaacgag catggtctgc
    10321 cgttttaagt aaaaaagaat ggagagcgac ttcaaaagtc gttctccatt cctatatatc
    10381 gtatctgtta tgtggatgct tacgctttgt tagctgaacc gaaccagtcg atatgagatt
    10441 tagcatcttc aacgattgct gctttacctg gagctaataa tttacgaggg tcgaatcctt
    10501 taccttcttt atctttacca gcttcgatat atttacgagt agctgcagca aatgataatt
    10561 ggaattcagt gttaacattt acttttgaga tacccattga gatagctttt tcgatttgat
    10621 cttgaggaat acctgatccg ccatgtaata ctaaaggcat gtctggacct acagcatcag
    10681 caattgcttg taagtggtca aaagctaatc ctttccagtt ttctggatat tgaccatgga
    10741 tgttaccgat accgcaagct aagtagtcga tacctgtagc aaccatttgt ttgcattctt
    10801 caatatcagc taattcgccg ttaccgatga ttccgtcttc ttctccgccg attgaaccaa
    10861 cttcacattc aactgagata ccttttgcat gtgctttttc aacaacgtct ttagctaatt
    10921 tcaagttttc ttcaaatggt agatgtgaac catcgaacat tactgaagta tagcctaatt
    10981 cgatacattc caaagcagct tcgtaatcac catggtctaa gtggatagca actggaacag
    11041 tgatgttcat tgcttcaact aaatcagtga ttaaatcttt acagattttg taaccaccca
    11101 tgtatttagc tgcacccatt gatgtttgga taagtactgg tgcttgtttt gcttcagccg
    11161 cttcaaggat tgcttgagtc cattccaagt tgtttgtgtt gaatccgccg acagcgtaac
    11221 cgcctttacg agcagctttt aaaaattcag ctcctgatac tactggcata ataaaattcc
    11281 tcctaagttt tgtaaaacta aatttgttaa gacaattctt aaccaacgac cctattttac
    11341 caaacatttc ggtagttttc tactaaaatc aatgaaaatg aaaattttca agaagttttt
    11401 tgcaaaaaat aaaatatcca atagcgcttt aatcgaaaaa agtcttttgc tttctatctt
    11461 ttttcctaca aataataaca aatcagaaaa agagaaaagt gacaagaaaa ttgcttatcc
    11521 ccttctcttg ctacttttct ctttctatca agttcaaaaa ttattttgtt tcgtgatctg
    11581 ctaatgatgc tccgacaaat gctttgatta atttttgcgg gcgatttgga cgagaaatca
    11641 attctgggtg gaattggcat cccacaaaga attttttctc tggcagctct acgatttcta
    11701 ctaaacggtt atctggtgag acacctgaaa agactagccc ttgttcttca aacaattgac
    11761 gatatttgtt attgaactca tagcggtgac ggtgacgttc ttgcacaaca tcggctccat
    11821 catatgcttc agccgtcttc gttccttttt tgattttaca tgggtataat ccaaggcgta
    11881 aagttccacc caaattctcg atgttttcct gatcagccat taaatcaata atattgtttg
    11941 ctgtatctgg attcgtttca gctgatgacg catcttctaa tccaacgacg ttacgtgcaa
    12001 attcgacaca agccatctgc ataccaagac aaatacctaa aaacggaacg tcattttcgc
    12061 gagcataacg aatcgcttcg attttccctt ctactccgcg atcaccaaat ccaccaggaa
    12121 ccaagattcc atctgcgtct ttcaaacgtt cttctacatt ttctctcgtt aattcttgtg
    12181 ctttgatcca atcgatctcg atgtctgaat tataggcgaa accagaatgt ttcaatgctt
    12241 caactactga gatatatgca tctggtaact cgacatattt accgacaagc gcaatccgta
    12301 cttttttctt caagttcaag acacgttctt ctaattcttt ccattctgtc atgtctgctt
    12361 ctggtgtatc tagttttaaa tggtcgcaaa caatttgatc cattccttgt gcttgtaaaa
    12421 ctaatgggat ggagtaaagt gtatctacat ctggcgactc gataactgct tctggttcga
    12481 catcacaaaa ttgtgccaat ttgtttttca cgctttgaga aacaggttgc tctgtacgta
    12541 ccaccaaaat attcggttgg atacctaaac cacgtagctc ttttacactg tgctgcgttg
    12601 gtttagtttt catttctcca gcagctttca agtaaggaat caatgttgta tggatataca
    12661 taacattatc tgtaccgaca tctgctttca tttgacgaag tgcttcaagg aatggcaaag
    12721 attctatatc ccctactgtt ccccctactt ctgtaatgat cacatctgaa tcagtcattt
    12781 tagctgctcg catgattttt tctttgatct catttgtaat atgaggaata acttgaacag
    12841 ttgctcccaa gtattcgccc ttacgttctt tacgcaaaac ttcagaatag atttttcctg
    12901 tcgtcacgtt ggagtatttg ttcaaattga tgtcaataaa acgttcgtag tggcccagat
    12961 ccaaatctgt ttcagcacca tcatctgtta cgaatacttc tccgtgctga tatgggctca
    13021 tcgtccccgg atccacgttg atgtatggat caaatttttg gatagttact tttaagccgc
    13081 ggtttttcaa taagcgtcct aaagaagctg caacaatccc ttttccgatt gatgaaacga
    13141 caccgccggt cacaaaaata tattttgtca taagttcaaa aactcctttt taatttatgt
    13201 gcaggaaaag tctcagacaa taaacaaagc tccctattct tcaataagaa tagggagccg
    13261 aaatttctgt aaacttctga ccctctaaaa gggtgcccaa ggtgtataat acaagctgac
    13321 taagggaaag tcaagtactc aaatgaatac tatccgctta caattttttc tttttcgata
    13381 tcctttgaga aattatttct taagtccttt atgagtgcaa aaaaaattta acccacataa
    13441 taagcttgat ataaacatag ctaacgttaa acctaagcaa atcagaaaat cgttactgaa
    13501 aaattttaag tgaaataata gataaaaaaa taaaacaaac attatgatag ataatatccc
    13561 acctaacaaa gaactacttt ttcctacttg cagcaacagt ttatatagct tatcgtagaa
    13621 acaatctttt tctttcaccc ttatttacct cctttacgta aaaaacgtag tccacagttc
    13681 tcggattttt ggcatatctt ttctattaaa taatatcaaa aacaaaacaa ttccactaat
    13741 tttttcaaag atgacctttt tttcaacaaa agtgccttgt agggattcat tgaaggtaac
    13801 gaatcacata agtaaaaaat aaggagatgc gacaaaagtc gtgcctcctt atttctaaat
    13861 aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatccatca aacatgagaa
    13921 gctgtttttg aaattatttc ttgtgattcg gagttaaatg gtaggctcac aacctctgga
    13981 taaacggt
//
LOCUS       DACDYJ010000065        13953 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDYJ010000065 DACDYJ010000000
VERSION     DACDYJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.065"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            212..2377
                     /gene="acm"
                     /locus_tag="IWM51_002103"
     CDS             212..2377
                     /gene="acm"
                     /locus_tag="IWM51_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP9971506.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2649..3134)
                     /locus_tag="IWM51_002104"
     CDS             complement(2649..3134)
                     /locus_tag="IWM51_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP9971507.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3614..4255
                     /locus_tag="IWM51_002105"
     CDS             3614..4255
                     /locus_tag="IWM51_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9971508.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4274..4909
                     /locus_tag="IWM51_002106"
     CDS             4274..4909
                     /locus_tag="IWM51_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9971509.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4927..5742
                     /locus_tag="IWM51_002107"
     CDS             4927..5742
                     /locus_tag="IWM51_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9971510.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6061..7497
                     /locus_tag="IWM51_002108"
     CDS             6061..7497
                     /locus_tag="IWM51_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9971511.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7686..10133
                     /locus_tag="IWM51_002109"
     CDS             7686..10133
                     /locus_tag="IWM51_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9971512.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10363..11430
                     /gene="rlmN"
                     /locus_tag="IWM51_002110"
     CDS             10363..11430
                     /gene="rlmN"
                     /locus_tag="IWM51_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9971513.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11623..12726)
                     /locus_tag="IWM51_002111"
     CDS             complement(11623..12726)
                     /locus_tag="IWM51_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9971514.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13279..13827
                     /gene="aac(6')"
                     /locus_tag="IWM51_002112"
     CDS             13279..13827
                     /gene="aac(6')"
                     /locus_tag="IWM51_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9971515.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4789 a         2306 c         2949 g         3909 t
ORIGIN      
        1 ataaaatgaa tatatgcgaa aaaataattt acattaggaa aaaccttgta aaaacagttt
       61 tttggcatat ttatttttta taaatatttg taaaataact atcaattttt tagttaaaag
      121 attggaaatg agtgggggag gaattgaaga aaggattttt gagagatgat atagtaggtt
      181 atttttagtt aaaatagggg agatagaaaa aatgaaaaag tgtagaaata ttattttatc
      241 aatgttattt attattacta atataacatc actgattcct gttcatgtct atgcggatgc
      301 aggcagagat atcagcagta atgtcacttc gttgaccgtg gacccaacga atattacaga
      361 tggaggaaac ataaaagtaa aattctcgtt tgacgaaaaa aaacaaaata tccaaccagg
      421 tgattatctt tggatcaatt ggccaagtga aggaaatatt cgcggtgaag gattccaaaa
      481 agaaatccca ttaatgattg aaaataaaaa tgttggaact ttaacggtca gaaaagattc
      541 tgcgcaggtt gtgtttaatg aaaatattaa aaacctagac tcagtagaag gttgggggca
      601 atttgagata caggccagaa acgtaaccga taccggtgaa gaaaatacgg gaccattcac
      661 cgtgacgagc ggtgataaaa cagctactgt aaatgtgaca aagccagctt ctggttcttc
      721 ttctagcgtt ttttattata aaacaggaga catgctacct gaagatacta agcacattcg
      781 atggtttttg aatattaaca ataacggcac ttatgttgaa caaccagtta aaatcagtga
      841 tgaaatccaa agtggacaaa gattagatcc aagtacgttt gaaattaatc aaatacactt
      901 aggtgaacag aaggtttata gaggagaaga gggaatccaa caatttttac aagatttccc
      961 cagcgctaca tttaattttt cagtaactga taattacatt gaaataacta taccaaaaaa
     1021 ctttgtcaat cttaggaaaa taatggtgag ttataagacg atcatagaaa acccagaaca
     1081 aataaatttt gagaatcatt cggaagcttg gtttaaagaa tttaataaac ctgctgttga
     1141 tggagaaagt ttcaatcata cagttaaaaa tatttcagct tctggtggag tcaacggtac
     1201 agttagaggc gaattgaaga ttttcaaata tatcaatgac acagagattg gtatcccaaa
     1261 tgtgactttc gaattaagac gagcagatga acagcctatt caaggacagt ctagtatcct
     1321 gttgacttcg aatgaacagg gagaagctag tatcaaaggc ctgcaagtag gagactatgt
     1381 tgtaaaagaa aaagaagcac ccaattggat tgattttgat ccactaagtt caaatgaact
     1441 caaattctcc ataaacgaaa atgatacaga gggagtcagt ttacctattt ataacaagaa
     1501 aaaggttacg aacatcacag caactaaaat ttggaacggt ggaaccactc caagaccgag
     1561 tatttatttt aaattattca gagcaagtac aaacaattgg gaaccagttc ctgatgcaga
     1621 aacaaaacga ttagacaatg gaataacttc agtgacttgg gaagatatcc aacagtatga
     1681 tgatagtgga aacgaatata cgtttaaagt gcaagaagta gatcaaaacg gaaatgatta
     1741 tgtcccttca ggttatcaaa aaattgaaaa cgggctagtc gttacaaatg agaataaaga
     1801 agtcatttca gtctcaggac aaaaaacatg ggaagacaat gagaaccaag acggcaaacg
     1861 accgacgacg atcacagtaa atctattagc cgacggtcaa ccgatccaac acaaagaagt
     1921 aagtgaaaaa gatgattggc gctatcgttt cacaaatctg ccaaaataca gagacgggca
     1981 agaaatcatc tatactgtaa cggaagacaa cgtgcccgaa tacagtacga cgattgacgg
     2041 gtataacatc aaaaacagtt atacaccgga aaaaacaaat atatctatac caggtagcca
     2101 tataacaccg tggaaaaatt ttccagagaa gacagagaga gagaaaatta gaaatctatt
     2161 ctctcaaaaa ttgcaaatat ttgctcatac aggagaatcc agcgaacaaa gaagcaatta
     2221 tattagtaag agagctgtac ctaaacagat aacaaataaa tcaaaaagca ttcttcctaa
     2281 aacaagtagt aaaaaatcta gttttgctgt aatcattggt ttattgatag taactatctg
     2341 tgctgggata ttcttacaaa aatataaaaa agtttagtta gaatataata ttttttgtta
     2401 aatatgactg aaatcaaaag aataaaattc cgatttataa tcgaaaaaag aagcattttt
     2461 atatatgctt cttttttcgt agaatcaata aatatggatt tggctaacta tggaagaata
     2521 ctcgtattag ttttcagcgt tcatactagt tgttacattg tttgattggc caaaagaatt
     2581 tctgatatat tttgtgctaa ataaagaaaa agacgcttga attgggaaat aagaaaacta
     2641 ctctatcatt acaaattttc ataaactggc agatgttcta agtaaacaag tatcttttca
     2701 aagtcggaaa aatccataat caacgtagtg gtattgatat ttggatgaaa gcctacggta
     2761 tcttgacgat caatagcagc atcgatcacg acttggattt ttttctcttt gtcgtgcatc
     2821 aatgctaatg gagtcactgt acctgctgga acgcctagga ggtcaggaag ctgttcttca
     2881 gaaacaaaag ataagcgctt ttcttttaat tgttcagcta gttgttttaa atcggcgtgc
     2941 ttttcatcag gcaagatgac aagatagatt tgttttcctt ttcttgattt caataataga
     3001 ttttttactt gcggaccagg tagggaaaaa gggacatttt tgactgacgt aatcgcagga
     3061 tgatcgactt tttgaaaaga gatacccagt ttttttagta gttcataagc ttcttgttcg
     3121 gttgcacgac tcatcgcgca tcaccctcct ttttttatta gtgtatcata aaaaagagtt
     3181 gacaaatgaa aaataatcat gtaaattaca cgtaaagtta aaacaaatga catcgaaaag
     3241 aagagtagcc gttaggtgtt tttagagagt ctctggttgg tggaaagagg cagcagttaa
     3301 ccgtgaaaca catcttggag tcgattttct gaagcgtata gtaggaaaat ccggggagcc
     3361 cgttacagct gcggtcgttg ctagatgacc agataaggct ggcttttccg tgaggagtcg
     3421 gtaaaaaaag gtggaaccac gtgattaacg tcctttgatc ttcggatcaa aggacttttt
     3481 gttttcatct gaacggcaac gactgtgtga ggttttttct gcaaggaaaa aataaacaga
     3541 ggtggaacca cgtataatac gtcctcttgg catttgccaa gaggattttt ttattttaaa
     3601 ggaggaaaag aagatggagt atttgattga agttatgccg caattattag taggagcact
     3661 cacaacattg aaattatttt tatttacctt gattggctca ttaccattag ggattgtttt
     3721 agcattagga ttgtctttgc attttaagcc gctacgctac ttgcttcaaa catatgtatg
     3781 ggttatgcgg ggaacaccgc tgttgttgca actgattttt attttttatg ggttgccgac
     3841 gattgggatc gtttttgatc gtttcgaagc agcactcatt gcctttatct taaactatgc
     3901 agcttatttt gcagagatct ttcgtggagg gatacaatcg attcctcaag gtcaatatga
     3961 agcagcgcaa gttttgcaat taagtccctt tcaaacggtg acgaaaatcg tcatcccaca
     4021 agtgacaaag attgttttac cttctgtagg gaatgaagtt atcaacttgg tcaaagattc
     4081 ttccttgatc tatatattag gtttaggtga cgtaatgcgt gctgggaaaa tcgcgatgga
     4141 acgcgacgcg actttactgc ctttgctagg cgtggcaatc atctatctta cattgactgg
     4201 tattctaaca atcgtaatga aacaagttga aggaaaatta aattattacc gctaactgat
     4261 aggaggaaga aatatgattg aattgaagaa agtgaacaaa gcatttggtg aaaaaaaaat
     4321 aatcaaagat ttgaatctaa tcattccaga tgggaaaaca ttagctatcg taggtccttc
     4381 cggtggcggt aaaaccacgc ttttacgtat cttggctggc ttagaaagtt ctgacagcgg
     4441 agaattttat ttagacggtg taccgttcaa cccattcgag cttaaaatga aagaacaagt
     4501 tgtaggagtc gtattccaag acttccagct atttccacat ctgaatattt ttgaaaacat
     4561 taccttagca ccaaaaatgg tattgaaaca gacaaaagaa gaatacacca aaaaagcaga
     4621 agaattagcg gcttcattag ggattagtga tttattgaat atctatcctt atcagctttc
     4681 tggcggacaa aaacagcggg tagcgattgc cagagcattg gctatgaatc cacgggtatt
     4741 ggcttatgat gaaccaacaa gtgctttaga tcctgaatta cgccagcaag tcgagcgttt
     4801 gatcctatca ttgaaagaag atggggtaac ccaaattatt gtcacccatg atttagtatt
     4861 tgcagaaaat gtcggtgatc aattactaaa agtttcccca attcattaag aaaggagaag
     4921 atagagatga aaaaaaccag ttttctattc gctttgatcg caggtcttct tctttttgca
     4981 ggctgttcga acaaaaaaac ttcagcagat aattgggcga ctatcaacga agagaaaaaa
     5041 atcgttatag gtttagacga tacatttgtt cctatgggat ttagagataa agacgggaag
     5101 ctcacaggat ttgatattga tttagccaaa gcagtctttg aacaatatgg gattacggta
     5161 gactttcagc ccattgactg gtcaatgaag gaatttgaac tgaacaatgg aacgatcgat
     5221 ttgatatgga acggctattc caaaacgccg gcaagagaaa agaaagtgca atttacaaaa
     5281 ccttatatgg aaaacgatca agtgctgatc acacctaaag aatcaggaat cacaaacttt
     5341 ggacaaatga ataataagag attaggtgcg caaaatgggt catctggtta tgatgtattt
     5401 acgaaacaac cagaagtatt aaaagagata gtcaaaaacc aagatgctgt tttgtatgat
     5461 agttttaatg aagcattgat cgatctgaaa agcagacggg tcgatggtct gctgatggat
     5521 aaagtatacg cagactatta tttgaaacaa agaaacgaac tagaagactt caacataaca
     5581 aaaggttctt atgagagtga ggattttgca gttggtgcac gcaagtcaga tagtacatta
     5641 gtgaaaaaaa tcaatgaagc cctagatcaa ctagagaaaa caggtgaatt ccaaaaaata
     5701 tccgataaat ggtttggcga agatgttacg ccgagaaatt aacaaatagg ggactgtgac
     5761 aaaagacgtg tcacagtccc tttttccgaa taaacagtgt tctcctgcca actccttgtg
     5821 actcggagct gaatggcagg ctcacaacct cttggtaaac ggtgttcaaa agccagtttt
     5881 tcggtattaa gccaaattta tgcaaaaatg tagaaagcca ttttcacaaa atttttctta
     5941 ctactcgaaa ctaagcgact ttttcacaac ctcctttaca tatttttatt ttgttgtgga
     6001 aggggtttcg tgttattcta aaagaggaac gattgaaggt tataggaaag gaagagatga
     6061 atggaacaca aagaacttac ctcatttcca aaagattttc tgtggggctc tgcttctgca
     6121 gcgtatcaag tagaaggagc ttggcaagaa gatgggaaag gagaatcagt ctgggatcga
     6181 tttgtacgga tacctggaaa gacatttaaa ggaacgaacg gtgatttggc agtagaccac
     6241 tatcatcgtt ataaagaaga tattgcttta atgaaagaac aaggtctgaa agcttaccgc
     6301 ttctctgtag cttggacacg gatttttcct aatggacgag gagaagtcaa tcaagctggg
     6361 ctcgcttttt atgagagatt gatcgacgaa ttgattgaaa acgatattga acctatgttg
     6421 actttatatc attgggatct tccacaagca ttgcaagatg aatataatgg ttgggaatca
     6481 cgtcagatca ttgaagattt cactcattat gcagaaacat tatttgaggc attccgcggg
     6541 aaagtccatt actggattag tttgaatgaa caaaatatct tcacttcatt aggttatttg
     6601 ttagcagcac atccgccagg tgtgacagac ccgaaacgaa tgtatgaagt taaccatatt
     6661 gctaatttag caaatgcatc tgtcatcaat aaatttcacg agttagaaat gcctggaaaa
     6721 atcggaccaa gctttgcgta tacgccaaat tatccaattg acagtaatcc tgaaaatgtt
     6781 ctggctgctg aaaatgcaga agacctgatg gccaattatt ggatggatgt ctatatgtgg
     6841 ggaaaatatc cgattgctgc catgaggttc ttagaagaaa aaggatgggc accaacgatt
     6901 gaagctggcg atgcagaatt gctagaatct gctaaaccag attttcttgg gatcaattac
     6961 tatcaaacgg cgacgaatgc attcaatcca ttaaacggag ttggtgctgg aaaaatgaat
     7021 acgacaggaa aaaaaggttc atcagaagaa actggagttc ctggaatgta taaaaaagtt
     7081 gagaatccat ttgtcgaacg aacaaattgg gactgggaga ttgacccaga aggattaagg
     7141 atcggactgc gtcgtatcac aagccgttac cgcatgccgg tactgattac ggaaaatggc
     7201 ttaggagagt atgacaagtt gacagaagat aaacaaatcc atgacgacta tcgaatcaat
     7261 tacttacaaa gccacgtcaa agccatcaaa gaagcaattt cggatggagc agaagtccta
     7321 ggctattgca cctggtctta tacagatttg cttagctggc tgaacggtta tcaaaagcga
     7381 tatggatttg tatatgttga tcaagacgaa acacaagaag gatctttgga acgatataag
     7441 aaagacagtt attactggta ccaaaaactg attgaagaaa atggagagaa cctttagttc
     7501 acatacttaa aacaagaagg tacggctgga tatggccgtg ccttttcttg ataaaatcat
     7561 ttagaaatta agaaaaactg taaaataagc tgaaaaatga aagaaagcgt cagttctttc
     7621 aaacaaaact ttagttccct tacttttttt tgtataatga aacttgatga aatgagggat
     7681 aataaatggc acaaaagaaa cggcctacta aaaaaaggaa aaagacgaaa aaaacgaaaa
     7741 agcaaaaaca gcagcaggaa caaatgctga cgattggtgt tgggtttgcc ttgattttat
     7801 ttgctgtctt cggcttttta aaattaggtt ttttaggaat attgattgca aacggctttc
     7861 ggatcattgc aggaaatact taccaaatcc tctgtctttt attagccgta ttaggatttt
     7921 ggattgtcat taagaataca gagttttcaa ttggtaaaaa ccgacgctgg tttggcggca
     7981 tactctttta tttcggtatc ttattattgt tacatgccca tttatttggc aaactccata
     8041 ctggggaacc aaatatcatg gggacgacat gggatttgtt agccagtgat ataaaacaaa
     8101 gtcaagtaga caataacgtt ggtggtggga tgattggagc aatcctttat cattttactt
     8161 acttcttgat tgcacagcct ggaagttatt tagcagcagt gctattactg gcaggaggag
     8221 cattcctgtt cagtaatctg gaaggctatc agctgttaaa tggtatccaa tcaattggag
     8281 aacgagtaca ggaattattg gaaggtgatc ctgccaagca agctcgaaag caagcggcaa
     8341 aggaagagag aatgaaacag cgcgcagaag cgaaagaagc aaggcgctta gctgcaaaag
     8401 aagctgcaga aaaagaagct gcagaatatg agaagaaaaa agcggtgagc cagcaaaggg
     8461 aaaatactgc tgatgaatgg caggaaccag aaaatacaga gccggagcag ctaagttttg
     8521 ttccaatcga cagttttcaa gaaaatatcc atcctgcgaa tctagagaaa ccagtaccag
     8581 atacaccaaa acagaccaat acagcagaag gtttttcaga tgagttgcca gaggatgacg
     8641 gtacctcatt agaatttgag attgaagcag aacaggaaaa tcaagactat gaattaccga
     8701 cagttgattt gcttgattct ataccaactg tggatcaaag tgatgagtat aaaaaaattg
     8761 aaaaaaatat cggcgtatta gaacaaactt tccaaagttt cggtgtcgat gccaaggtcg
     8821 tcaaagccag cttaggacca tccgtcacaa aattcgaagt ccagccggct gtaggggtaa
     8881 aagtaagtaa aattgtgaac ttaacagatg atatcgcgct tgctttagcg gcgaaagatg
     8941 tccggatgga agcaccgatt ccaggtaaat cactgattgg tattgaagta ccaaatggaa
     9001 agatcagtat ggtttccttt agagaaatca tcgaagcaca accaaaccac ccagataaat
     9061 tactagaagt acctttagga cgtgatgttt ctggacgggt acaaacggct gatctttcga
     9121 agatgcctca cttactcgtg gctggttcga ctggtagtgg gaagtcagta gcaattaatg
     9181 ggattatcac aagtatatta atgcgtgcta agccccatga agtaaaactg atgatgatcg
     9241 atccgaaaat ggttgaattg aatatgtaca acggcatacc tcatctgttg acgccggtcg
     9301 tgacgaaccc tagaaaagca gcgcaagcct tacaaaaagt agtacaagaa atggaagaac
     9361 gatatgaaaa attcgcggca accggagtaa gaaatatttc tggttacaat gaatttgttc
     9421 aacagaaaaa cttggaaaac ggaacgaaac atccaacctt accattcatc gtagtgatcg
     9481 tagatgaatt agctgatttg atgatggtag ccagcaatga agttgaagat gcgattattc
     9541 gtttagcgca aatggctcgg gcagcgggga ttcatatgat cctagctact caacgtccaa
     9601 gtgtggatgt tatcacagga atcatcaaag caaatgtacc ttccagaatg gcatttgcgg
     9661 tatcaagcgg tacagattct cggacgatca ttgattctaa tggggcagaa aagctcttag
     9721 ggcgaggaga tatgctattc ttgccaatgg gtgagaataa accgatccgt gtgcaaggag
     9781 cattcatttc tgaccacgaa gtagaacggg tcgttcaatt tgttacagat cagcaagaag
     9841 ctcattatga agagaaaatg atgccgacag atgaagttga aacgacaggt gctccagaac
     9901 agccacaaga tgagctgttt gaagaagcaa aagcacttgt cgttgaaatg caaacggcaa
     9961 gtatttccct tctccaaaga agatttcgga tcggttacaa ccgagcagca cgtttagtag
    10021 atgaactgga agctcatgga gttgttggcc cgtcagaagg aagcaagcct cggaaagtat
    10081 tcattgagca ggaagaaaca tcagaaacgt tgccggacga taatgacatg taaaacttgt
    10141 gtgatgaagg gttgaaaaac gaataaccaa taaaaagaca aggcatgatg ataccaaaag
    10201 aaaaaatggt ttttttccat cttttctaat acggtatgat gtcttgtctt tttagttttt
    10261 gtgagattaa agttctattc gatttatttg tgaagtattg cattaaggaa atgaatcatg
    10321 tatagtatgt agcgaaactt tgagaaaaag agggaaaata ctttggaaaa acagtccatt
    10381 tatggcttga cgaatgaaga attaatcaat tggtttatag aaaacggcga gaaaaaattc
    10441 cgagctgccc aagtttggga atggttgtat caaaaacgtg taagtaattt cacagaaatg
    10501 acgaatctat cgaaacaatt gatcgaaaaa ttatcggagc attttatcat caatcctcta
    10561 aaacagatgg tcgtccaaga agcttcggat ggtacagtaa aatatttgtt tgaactccct
    10621 gataaaaaca tgattgagac tgtactgatg agacaagaat atggcttgtc agtttgtgtg
    10681 acgacacaag taggatgtaa tatcggctgt acgttttgtg caagcgggct gctcaaaaag
    10741 aaccgtgatc tgactgctgg agaaattgtt gcacagatca tgatggtcca acattatttt
    10801 gatgagcgca atcttggcga acgcgtttca catgtcgttg taatggggat cggtgagcca
    10861 tttgataatt acgacaatgt aatggacttt ttgcatatca tcaatgatgc caaaggattg
    10921 gcaattggtg ctcggcacat tacagtttcc actagtggat tagcacacaa aatcaaggaa
    10981 ttcgctaata acggcttgca agtcaatctg gctatttcct tgcacgcgcc gaataatgag
    11041 gtgcgaacat cgatcatgcg gatcaaccga agtttcccaa ttgagaaatt gatggaagct
    11101 gtggatgagt atttagagaa aacaaaccgt cgaatcacgt ttgaatatat catgttgaat
    11161 caagtaaatg atagaccaga gcatgcgcaa caattagcag atctattaaa agataagaaa
    11221 aaattagcat atgtcaactt gattccatat aatccagtca gtgaacatga tcaatattct
    11281 cgcagtccaa aagctgatgt attgagattt tatgatgtat tgaagaaaaa cggcgtaaac
    11341 tgtgtgatac gtaaagaaca tggtacagat attgatgcag cttgcggaca attgcgaagc
    11401 aagcaaatga aaaaaacagt tgccaaataa tcgtatccta ctcgtttttt caattaccat
    11461 aaaaggagca gttttcttaa ttaaggaact gttcaactta aataagagaa aagattctat
    11521 agcgaaagct ataaagcttt ttttgacaag aaaaaccctc atcaagcaaa gaaatgtcct
    11581 caatttgatg acagcctgct tgatgggggt tttagcaatt atttaactga ttttcttata
    11641 tccttgctgg ctttcttgtg tagcatgata agtttctacg tcaagaatgg cctgtttaaa
    11701 tttttctcgg gtgatttcat aaggcacatg tttaatatca tttgttgcca tggctttatc
    11761 taagattgct tctaaatcgg tcgtgacatt gagattcatg tcggttaacg atactggaag
    11821 ttggttcttt ttcataaaca catagatttt atcaaacaaa tcgaactgct gatccatcat
    11881 caataaggtc agtacaccat aactgacaga tgctccgtga aggtatccct ccattttttc
    11941 tagaacagtc gtaccgtaat agaatgagtg tgccatagca gaattatagt tattgttaac
    12001 taaaacagaa accaatccgg tagtaacaat gatatccgaa acgacttgtt caattgcata
    12061 tgagggacga tcgtcggcac tatctttcaa tgcttgcgta ccataagtca agagacgatc
    12121 aatgcacatt tttccgattt ggattcctaa tctatccgtg tagcctagat catcgccacg
    12181 agcagaaaaa gtagattcga cttctttgga taaagcatcg ccaatacctg cccataagta
    12241 tttatcaggt gcttcggcaa taattgctgt atcaatgaag gtgtgtacag gtggttttga
    12301 aggaaagaag agtccgtcca ttacgccttc ttcgttatag atgacacata ccgcagtgac
    12361 gggcgaacag ttcgaaccaa tagtgggaaa ggtgaagact ggtttagcta gtttttcacc
    12421 aatgactttt acagtatcgc aagcacggcc accaccgact gcaaacagga cgtctgcttt
    12481 ttggacactt tcatgctgga tcaactgttc aatatttcca taagttgaat tacccccgta
    12541 ccagagactg gcgacaacct ctatttcctg tgtcaaagct acttttattt tgttttctgc
    12601 ctttgaaagt gcagtcttcc ctccaatcag cactactttt gtcccatatt ctttgacgat
    12661 tccatttgct ttttcatacg catctgttcc aatggtataa tttggtaaga aaacactttg
    12721 actcatattc atttccttct ttcttcgttg aaacgggtta aaattttaaa gagtgatcga
    12781 tctgactgcg gctgtagatg gagtagaggt gaacctaaga caggataagt gatgaataag
    12841 gtggcatgaa caccttgaaa tcatcaataa gtatcctaaa aacgaacaag tacaaaaaca
    12901 ttgaaaacga caataatgaa tttttgttcc tcttagtatt tctaccgatt ccctttgctt
    12961 ttttgtaatt atctaaaaat tctaaaaatt aagattatta tatgaaaaca ggttttattt
    13021 gtcaattcat aattggtttt ttttaaagca aaagataatc tatatcatga tgaacaaaca
    13081 gagttttttc ctccaattga tggctttcct gatctttcct attttgaata aagtgtttct
    13141 gtataatttt tttattttat aagtaagata aataatgaaa gaatagagat actgattggt
    13201 agctaaagat ttttgagcga gaacatttcg ttttttcaaa aggactgtta gaataagcaa
    13261 gaacaagggg gaagacgtat gataatcagt gaatttgacc gtaataatcc agtattgaaa
    13321 gatcagcttt ctgatttact gagactgact tggccggaag aatatggaga cagctcggca
    13381 gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg tagcagcggt agaccaagat
    13441 gagttagtag gatttattgg tgcaatccct caatacggta tcacaggttg ggaattgcat
    13501 ccattagttg tagaaagctc ccgacgaaag aaccaaatag gtactcgatt agtcaattac
    13561 ttagaaaaag aagtagcttc cagaggagga atcacgattt atttaggtac ggatgattta
    13621 gaccatggaa caacgttaag tcaaacggac ctgtatgagc atacatttga taaagtggct
    13681 tccatccaga accttcgtga acatccgtat gaattctatg aaaaattagg ttataaaatc
    13741 gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg atatttggat ggcaaaaacg
    13801 attattcctc gaccaaattc tcaataataa aaagaggttg tgacagaagt aatcagcttc
    13861 aagaaataag aaggaattta cgaaaattac ttcttgaatt tttgtgaaat tccaacttat
    13921 ttccgaagaa gctacttttg gaacaccgtg tac
//
LOCUS       DACDYJ010000066        13944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDYJ010000066 DACDYJ010000000
VERSION     DACDYJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.066"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            949..1422
                     /locus_tag="IWM51_002113"
     CDS             949..1422
                     /locus_tag="IWM51_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP9971516.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="IWM51_002114"
     CDS             1431..1658
                     /locus_tag="IWM51_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9971517.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="IWM51_002115"
     CDS             2294..2665
                     /locus_tag="IWM51_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971518.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="IWM51_002116"
     CDS             complement(2921..3163)
                     /locus_tag="IWM51_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9971519.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="IWM51_002117"
     CDS             complement(3195..4097)
                     /locus_tag="IWM51_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9971520.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="IWM51_002118"
     CDS             complement(4110..4298)
                     /locus_tag="IWM51_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9971521.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IWM51_002119"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IWM51_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9971522.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="IWM51_002120"
     CDS             complement(4913..5806)
                     /locus_tag="IWM51_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9971523.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6822)
                     /locus_tag="IWM51_002121"
     CDS             complement(5884..6822)
                     /locus_tag="IWM51_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9971524.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="IWM51_002122"
     CDS             complement(6856..7206)
                     /locus_tag="IWM51_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9971525.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="IWM51_002123"
     CDS             complement(7239..8141)
                     /locus_tag="IWM51_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9971526.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="IWM51_002124"
     CDS             complement(8134..8991)
                     /locus_tag="IWM51_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9971527.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="IWM51_002125"
     CDS             9325..10134
                     /locus_tag="IWM51_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9971528.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="IWM51_002126"
     CDS             10174..10671
                     /locus_tag="IWM51_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9971529.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11056..11129)
                     /locus_tag="IWM51_002127"
     tRNA            complement(11056..11129)
                     /locus_tag="IWM51_002127"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11093..11095),aa:Ile,seq:cat)
     gene            11316..11639
                     /locus_tag="IWM51_002128"
     CDS             11316..11639
                     /locus_tag="IWM51_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971530.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11803..12057
                     /locus_tag="IWM51_002129"
     CDS             11803..12057
                     /locus_tag="IWM51_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971531.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12127..12372
                     /locus_tag="IWM51_002130"
     CDS             12127..12372
                     /locus_tag="IWM51_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9971532.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12448..12813
                     /locus_tag="IWM51_002131"
     CDS             12448..12813
                     /locus_tag="IWM51_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971533.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12874..13437)
                     /locus_tag="IWM51_002132"
     CDS             complement(12874..13437)
                     /locus_tag="IWM51_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971534.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         4088 a         2577 c         2304 g         4975 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca tctttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    11041 tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
    11101 tctgctctaa ccaactgagc taaaggtaca tcataaataa aatttatttt tgttactttt
    11161 caactatagc acgaatgaca aactctcaca agtttcaaaa tataagtaaa ataatattgt
    11221 tattcttatt tcttttactt gtgtcataac tattttgtta ctttaatttt tatttgctta
    11281 ttttagaaaa aatgttattc ttttatttat tctcaatgtt aggagtgatt attttgccat
    11341 ttattgcgat tttattcgat ttagttgcgg cagcagctta ttttctccaa ttaaattatc
    11401 aaacctcagg aattctgttt attggaatga tttttcaagg tgtgattacc cttttgcttc
    11461 ttgtcatgat gctcagctat aaaggaaaaa gatatgcccg tattcaaaca aaagtatttg
    11521 ttaaatatgt ttccattcgc tatgggatca tcattctttc ctttttagtg aatgcgatcg
    11581 tcctcttttt atatgtattg aattacttag atatcaatcc attgattttt tctcgataga
    11641 taagacacat tcagcggaac agttcctgct tatcagcaga aaactgttct gctttttcat
    11701 ttttcattga tctattcttc ttttattttc ctagcatttc atttgtgatg tatgcaataa
    11761 catgttatct tttattatat gaatcgttga ggagtccgtt acatgattac gttggtgaga
    11821 gttttgtttt gggtgcctgc ggtcgcactg gtcgcaagta ttgtttatct tatgaattgg
    11881 aacaaagaac ggttttattt agctattttg actcttccgg ctatctactt tatgtggaaa
    11941 gtttttaatt acaactactt tgaacccgat agtgttttta tagaagaatt atcaggactg
    12001 gttttgtctt tattgatcgt cattctttat ttgatacgtt taaataaaaa acattgagta
    12061 ctattgattg catgtgtagc tttctatagt cattataact attctactat aatgaaaggt
    12121 gattaaatga gtatcgaaga agctatttta ctggatactt tacgttgtcg tctgaatttc
    12181 ttaagaactg gccgatcttt atctcaagta gctgtttcta gcgcgttaag tattccttta
    12241 ttcaaatatc gtaagtacga agaaggagaa gaaattccaa gcatcattga attaatctat
    12301 atcgctgact tttacgatat ttccgttgat tacctaattg gtcgctcaga aacagcaagc
    12361 aaactgaagt gattttttcc agccttggtg ctggtttttt catttaacgt ctaaggttaa
    12421 caatctatat ataaaggaga catttctatg cgattatggc atgaaaaatt gatttcaaag
    12481 cttcctcgtc aacagctttt aggacaacat cgggaatgct gtgcattaag aggaaatggc
    12541 tggggaaaga aacatgctac tgtggattat gtttttcact attctcctta tagattattc
    12601 caatatcatc agcttgtcat ggatgaaatg gaaaaacgtg gttacagccc agcaccagag
    12661 tggaaaaatc ctatgtatcg tggcaaatca tgcgaagcat tttttgattt acctactgta
    12721 gagcgcaacg tgccgattta tccggaacac gatgaagttt atctagcaga atgtttagca
    12781 aatttggagc aaaaaggaat ttatcttacc taatataact agaaaagcct tctatctcct
    12841 cttacttcat taaaaagctt taaccatctt agatcaagct aaaatgtcgt aagttttagg
    12901 gttatcttca tcctcacgtg caaaactaaa agcgtactct agttttggtt catcgccaac
    12961 ttccctagac ataaatgaac atctcctgac gtggtttaaa agtaccgcat cttcgacgtc
    13021 tgtatacgat gcacagcaca gattgcttct tttgatcttt cttttgattt ttctgttgct
    13081 tcttctacac cttcttcgat tactacatcg atgcttacaa ttgcttctgc ctaatattcc
    13141 catgcatcgt cgatttaagc ctctgtagtc actgtgatcg tttccacatt ttaactcctc
    13201 tgtcgtggct tcagttgatt ttgtgatcag ttcagttgct aagccagagt cagaacaacc
    13261 tgccattgct agcgttaata ttgatagtaa aaatagtttt tttatcataa ggttcccccc
    13321 ctgaaaatag actaaaataa taacgctttc tttttttgtt ttcagacgaa aattaataat
    13381 ttctaaatat tctttaaaaa agcttataac tattatgcct tttttaatat ataacacagt
    13441 tatttttaaa aataagaatg ctaaatcact ctacaataac ggcgaaagca caaaaaataa
    13501 aaaaattatt acgttattaa aaaaacagat gaaagatcag gttttattag atctcattct
    13561 ttcatcactt gaagggattt aatgaactac tttttcctct ttgtcgtttt tatatattcg
    13621 tattccttca aacagtagga tagcctcttt caatgcttga tatttgaact gtccgttact
    13681 taaaaattta gatgtgccgc cctcttccta gaacatattc ttttttactt tttcattaat
    13741 tgacagacca atcgaacgaa actgtaaatt tcgataggat aatgattggt caaactcatt
    13801 cattttcaag acatctcgat tcatattcaa gataggaatc tttttatcaa catcacttta
    13861 ttcgtatttt cctctaccat aaaagcttca cgctccacaa aattgtttta tacactacta
    13921 ttcagcccat tcaacgacca atga
//
LOCUS       DACDYJ010000067        13916 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDYJ010000067 DACDYJ010000000
VERSION     DACDYJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.067"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            131..505
                     /locus_tag="IWM51_002133"
     CDS             131..505
                     /locus_tag="IWM51_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9971535.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IWM51_002134"
     CDS             complement(619..1248)
                     /locus_tag="IWM51_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9971536.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IWM51_002135"
     tRNA            complement(1382..1454)
                     /locus_tag="IWM51_002135"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IWM51_002136"
     tRNA            1668..1741
                     /locus_tag="IWM51_002136"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IWM51_002137"
     CDS             1696..1845
                     /locus_tag="IWM51_002137"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971537.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IWM51_002138"
     CDS             2338..3666
                     /locus_tag="IWM51_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9971538.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IWM51_002139"
     CDS             3868..4701
                     /locus_tag="IWM51_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP9971539.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IWM51_002140"
     CDS             4717..5454
                     /locus_tag="IWM51_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP9971540.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IWM51_002141"
     CDS             complement(5609..6577)
                     /locus_tag="IWM51_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP9971541.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IWM51_002142"
     CDS             6809..7642
                     /locus_tag="IWM51_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP9971542.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IWM51_002143"
     CDS             7676..8515
                     /locus_tag="IWM51_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9971543.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8540..9100
                     /locus_tag="IWM51_002144"
     CDS             8540..9100
                     /locus_tag="IWM51_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9971544.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9299..11020
                     /locus_tag="IWM51_002145"
     CDS             9299..11020
                     /locus_tag="IWM51_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP9971545.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11185..12327
                     /locus_tag="IWM51_002146"
     CDS             11185..12327
                     /locus_tag="IWM51_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9971546.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12343..13554
                     /gene="thiI"
                     /locus_tag="IWM51_002147"
     CDS             12343..13554
                     /gene="thiI"
                     /locus_tag="IWM51_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP9971547.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4750 a         2298 c         2833 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaatcagta tggaagcaga gacacctttt caaaaagtag aaacattcca
     8581 caaaactttt gatccaagaa gaccagagat acccacggct tttacagaaa atgaagctca
     8641 tacacgtgct gcgttcaaaa tagaagaact tgttgaattt gttgcagcta cttcacctaa
     8701 taaagaacaa ctgcatgaag ctgttatgct gctgcacgaa gcattagata aagcagaaga
     8761 aaaaatactt ggtaaaacac agtggggaag tgatccgctg gttgaacaag tagatgcact
     8821 gacagatatc ctttacttta cttatggttc tttttcatta ctaggaatag tgcctgatcg
     8881 aatattcgaa atcgtccatc aggcaaatat ggggaaatta tttccagatg gaaaacctca
     8941 ttatgaccct gtaacaaata aagtgttgaa acctgaaaac tggcagatgg aatatgctcc
     9001 agaacctaaa atcgcggcag aaatccaacg tcagattcaa gaagcgattg ctaagaagaa
     9061 atccgctgat ataggcatgt gcacaaatga aaaagagtga tcagagtttc actatttttg
     9121 ttatctttat acgatggaaa aacaaaagat tttcgtttaa gttagtgaat gcatgaaaat
     9181 agtatgctat aatctagaat agatgtcttt tgcacgagag acgtattatt tggatttatg
     9241 ccaagcagaa cgccatctgt tgcataaaag atgcatactg aaaacggagg aaccataaat
     9301 gggaaataat gttatcatcg gaatcgtcat tgcaatcatt atagtcgcca ttgtcgtctt
     9361 tttggttagt tacttgatga agaaaaaaaa ccaagatcga ttggatgcct tagaaaaacg
     9421 caaggaagaa ttatttgact tgccagtgat tgaagaagtc gatgatgtta aaaaaatgca
     9481 tctggttggg caaagccaaa atacctttcg tgaatggagt caaaaatgga atgacatatc
     9541 tacttcctcg ttcgctgaac tagaaagtca aattttcgaa gtggaaaacc tcaatgaaag
     9601 ttttcggttc ttaaaaggaa aaaaagcagt tgttgaagca gaagctacaa tggaccaaat
     9661 ggagaaacaa gtgacggaaa tccgtgaagg tctaaaggaa ttgcgggaaa cggaagaacg
     9721 caactcacta gctgttcagc aggcacttga cgtctatgaa gaactgaaag aaaaagtcag
     9781 agaagacgag catgaatacg gaccagctct ttttgaattg caaaaacaga tcaaagtgat
     9841 cgaaaaagaa tttacgcagt ttgtgacttt aaacacttct ggagaccctg ttgaagcccg
     9901 cgaggttttg gataaagcag aaaaacatac gtatgaagtg gaagctctga tgaaaaaaat
     9961 tccaccgctg tatgaggagc tccataccac tttccctgaa caacttgaag aaatcagtga
    10021 tacttacgag aaattgatgg aagaacaata tgtattccca gaagaagatc ttgctgaaga
    10081 tatcgcaaaa gtaagtcgaa gaattgaaaa ttcattagcc aatcttgaaa aaacagaagt
    10141 tgaaacggtc gaatttgaaa accgtgaaac agctgatttg attgattcgt tatatgatat
    10201 cttagaacgg gaaatggaag ctcagagata tgtaaaaact aaccaatcaa cgattgcaga
    10261 gtatatcaaa catacaacaa agaataaccg tcaattattg attgagctgg atcacacatc
    10321 tcaaagctat acattgaacc acaatgaaat tggccgtgta cgaggtttcc aaaccgaagt
    10381 ggaagaaatg gaacgtcaaa atgaacagat gattccacag atccgtcaac acgaaattcc
    10441 ttattcagaa gtgcgcacgt tctataaaac agtcttcaaa gtcttagaag atatcgaaaa
    10501 gcaacaagta gagatcgacg actcattaca tgaattgaga aaaggcgaaa aagaagcgca
    10561 agaaaagatc gatacattcg aattcaaatt gcgcagtttg aaacgttttg tagaaaaaca
    10621 acgtttgcct ggcttgccga atgattacct agaattcttt tttgttgcta ctgaccgtat
    10681 tgaagaacta agtgttgtct tgaataaaat tcgagtaaac atggaagagg tcaatcgttt
    10741 agttgctcta tgtgaagaag atctagaatt attagataag aagacacatg atttagtaga
    10801 tgcagcagca ttaacggagc aaatgctcca atatgcaaat cgttatcgtc atacacacga
    10861 agaagtgaga gaagcaattg ataaaagcta ctatttattc aataaagaat atcactatca
    10921 agaagcatta gacgaaatcg gtacagcatt agagcgagta gaacctggtg ctttcaaacg
    10981 catcgaagat ttttatttta atcatccaga cttagtataa agcaaaaaag ttagtaaacc
    11041 agcaagagca agacggatta ttgtcttgtt cttgtctttt tttgtatatt tgaaaattga
    11101 aacaagtgaa aatttcacct gctcttgaaa gcaccttgga caaccgttat aatagaaaag
    11161 aatttcaaaa aagaggggtt tcttatgata tattttgata acagtgcaac aaccccaatc
    11221 tttccgcaaa gcttagatgc atatgtaaaa accagtcagc ggattattgg gaatccatca
    11281 agcttgcatg atttaggcaa tcaagctaac cggttgttgc agcaagcaag aaagcagatc
    11341 gctgatttga tccatgtaga accagaagaa atctatttta cttcaggcgg aacagaagga
    11401 gataactggg tattaaaagg aacgatgatc gaaaaaagag aatatgggaa tcatatgatc
    11461 atttcaggag tcgaacatcc ggccgtcagc gagacagcag aacaattaaa agaattagga
    11521 atcgaagtaa gtatcgcccc agtggataaa cgcggatttg tacgtgtcga cgaattgaaa
    11581 gaattaataa gaaaagagac agtattagtc tctgttatgg ctgtgaataa tgaagtagga
    11641 tcgattcagc ccatccagga aatcagtgat cttttggatg attttcctaa aattcatttc
    11701 catgtagatg ctgttcaggc gattggaaaa gtaccattct cgcaatggtt gacagaaaga
    11761 gttgattttg caacattttc agctcataaa ttccatggcc cacggggaac tggtttcatt
    11821 tattggaaaa agggtcgtcg tttagcacca ttactgacag gtggagggca agaaagaaat
    11881 cagcgaagtg ggacagaaaa tgttccaggt attgtggcta tggttaaagc tttacgttta
    11941 tcattagaga taaaggaaca aaaacctgag cagacacaac gcttaaaagt agctctaatc
    12001 aatgcgttga agacgtatga gaaagtgacg attttttcag aaggaactga ctatgcaccg
    12061 catattttat gttttgctct aaaaggaatt cgcggcgaag ttctcgtcca tgccttagaa
    12121 gaaaaacaaa tatttgtttc tactactagt gcctgctcct cacgtaaaca tatggcaagc
    12181 agtacattgc acgcaatgca tgtaccaaat gatctggcta catctgctgt tcgaatcagc
    12241 ttagatgaaa acaattcgct tgcagaagtc gagcaattta tgattatttt caagcagctt
    12301 tataagaaat tttctgcaat caattaaaag aggagtgttc ttgtgaaata cagtgaaatt
    12361 atggtccgtt atggggaact ttcgacaaaa ggaaagaatc gaaaaagctt tatcatgcaa
    12421 ctagcacaaa atgtcagaca agctttgaaa gactttccag aaatcaaaat ccatgctgat
    12481 cgggaccgga tgcatctttt attgaatggc gcagacgggc agctagttat tccaaaacta
    12541 gcaaaaatat ttgggatcca aaatttctct ccaagtattc gtgtagaaaa agatgtcgaa
    12601 gttttgaaac aagctgttca agacatcatg aaagaaattt atactgggaa agaaacattt
    12661 aaaattatcg ctaaaagaag cgatcaccaa tttgaattga caagtaatga attgaatcaa
    12721 acattaggta atgctgtttt tgatattttc ccgcatatcc aagttcaaat gcgtcaacca
    12781 gatattccat tacgtgtaga gattcggcgt gatggcgctt atctatcgta tgaaacgatc
    12841 aagggagctg gaggattgcc cgtaggaaca agcggaaaag gaatgcttat gctttctggg
    12901 gggattgatt ctcctgtagc agggtacttt gccatgaagc ggggagtgga aattgaagct
    12961 gttcattttg ctagtccgcc gtatacaagt gaacaagcac tgcaaaaagc aaaagatcta
    13021 acagccaaac tagcacctta tgtgggaacg atccatttta tcgaagtgcc atttaccgag
    13081 attcaagaag aaatcaaaaa gtcagtgcca caaggatatt ggatgacaat tactcgtcga
    13141 atgatgttgc gtttgaccga tgaaattcgc cgtattcgtc atggtttagt cattttgaat
    13201 ggtgaatcat taggtcaagt ggcttcacaa actttgcaaa gtatggtggc tatcaatgaa
    13261 gtaacgaata cgccaatcat tcggcctgta gcgacaatgg ataaacttga aatcattgaa
    13321 gtagcagaac agatcgatac atttaatctt tctatccagc cttttgaaga ttgctgtacg
    13381 atttttgctc ctccacagcc aaaaacacgc cctcaactgg aaaaggtcca tcaatatgaa
    13441 gagcgtttag caatagatga aatgatccaa cgagcactag ctggcttgaa gatggaagag
    13501 atacaagcag aatcgtctaa tgaaaaaact gaagaatttt cagaattgct ataagaggtt
    13561 atgaaaatag tgttttgacc agagtattag agaaacaaaa cgaaaaaata gctccatgta
    13621 ttttaccgtt tttgaactaa tgtcgaagat tagctgattg aataccattt atcaagaagt
    13681 tatggaaaag ccggttcttt tttactaact gaaaaataaa attttattta gagatcaagc
    13741 tacagttgat aaatgatata aaaaagagaa tttcccatga aatagcgagg ttgtgacaga
    13801 agtgggcagc ttcaagaaat aagaaggaat ttacgaaaat tgcttttcaa atttttgtga
    13861 aatttcggct tatttttcga agaagttact tctggaacac cgtgtatcag aggttg
//
LOCUS       DACDYJ010000068        13700 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDYJ010000068 DACDYJ010000000
VERSION     DACDYJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.068"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..901)
                     /locus_tag="IWM51_002148"
     CDS             complement(<1..901)
                     /locus_tag="IWM51_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP9971548.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(925..969)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1184..2152
                     /locus_tag="IWM51_002149"
     CDS             1184..2152
                     /locus_tag="IWM51_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9971549.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2265..2543
                     /locus_tag="IWM51_002150"
     CDS             2265..2543
                     /locus_tag="IWM51_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9971550.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2661..2897
                     /gene="secG"
                     /locus_tag="IWM51_002151"
     CDS             2661..2897
                     /gene="secG"
                     /locus_tag="IWM51_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP9971551.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3009..3755
                     /locus_tag="IWM51_002152"
     CDS             3009..3755
                     /locus_tag="IWM51_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9971552.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3758..6115
                     /gene="rnr"
                     /locus_tag="IWM51_002153"
     CDS             3758..6115
                     /gene="rnr"
                     /locus_tag="IWM51_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP9971553.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6137..6601
                     /gene="smpB"
                     /locus_tag="IWM51_002154"
     CDS             6137..6601
                     /gene="smpB"
                     /locus_tag="IWM51_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP9971554.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6903..7622
                     /gene="atpB"
                     /locus_tag="IWM51_002155"
     CDS             6903..7622
                     /gene="atpB"
                     /locus_tag="IWM51_002155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP9971555.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7675..7890
                     /gene="atpE"
                     /locus_tag="IWM51_002156"
     CDS             7675..7890
                     /gene="atpE"
                     /locus_tag="IWM51_002156"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP9971556.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7981..8505
                     /gene="atpF"
                     /locus_tag="IWM51_002157"
     CDS             7981..8505
                     /gene="atpF"
                     /locus_tag="IWM51_002157"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP9971557.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8492..9034
                     /locus_tag="IWM51_002158"
     CDS             8492..9034
                     /locus_tag="IWM51_002158"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP9971558.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9057..10613
                     /locus_tag="IWM51_002159"
     CDS             9057..10613
                     /locus_tag="IWM51_002159"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP9971559.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10627..11529
                     /locus_tag="IWM51_002160"
     CDS             10627..11529
                     /locus_tag="IWM51_002160"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP9971560.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11552..12958
                     /gene="atpD"
                     /locus_tag="IWM51_002161"
     CDS             11552..12958
                     /gene="atpD"
                     /locus_tag="IWM51_002161"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP9971561.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12972..13394
                     /locus_tag="IWM51_002162"
     CDS             12972..13394
                     /locus_tag="IWM51_002162"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP9971562.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4530 a         2450 c         2988 g         3732 t
ORIGIN      
        1 tttcggaaaa aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc
       61 aaaattcata acgatcaacc aatgtttgtc cacgacttat tccctgagtt tccacagcgg
      121 tgaagctttt gaaaccttca cagatggccc cctcgatgaa ataagcaaca gctaacggat
      181 cattgatgac acatcctaaa attctttctt gtttccagtg gaagtcaaaa taaaaacgag
      241 tgatcgcttt taaatactct ccctcttcag gattcatttg acagcagtat tcgaggatag
      301 tcggtgtcaa gacgatttct cttgtcacat ctaacccaac catttctatc gtttgtttga
      361 gattctcgaa tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg
      421 gcgaacagtt accatggctt ttgtaagttc cacccatcga gacaaaacga tccatatgtt
      481 tcccaatatt tgggttgact ttcaaagcaa aagcgatatt ggtcaaagga cccaaagcaa
      541 tgactgatgt atttgtcttt tctttgaaat aatcagccaa aaaatcaacg gcatggagag
      601 gttctgcctg ttttctcaag atcatcggga aatttgtttc ccctaaacca tccataccat
      661 gagtgtcttg tgcactgatg aaaggttgct tcaaaggttt ttccattcct tgataaacag
      721 gaatgtctaa acgatcacag cgttccaggc atttcagcac attctgcgta cctatatctg
      781 cagggacatt cccacaaact acggtaatag caactacttc gatatcaggg gatttgacag
      841 ctaatgataa agctaaagtg tcgtcgatcc ctggatcgca atcaataatt acttttcgca
      901 ttatttccaa tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac
      961 cagtccaaga cgagcgtctt gattataatg cacgaaatta gtatactggc ttttattgcg
     1021 aatgtaaatg ctctcttaca agtaaatcta ttgaggagaa ctttaagtaa atgatacaat
     1081 gaaaaagaaa tggaatttac gtgtgccgaa aataaagaat cctatggatc ggtacattgg
     1141 ataatagata gattatagtt tggcagaaag cggcgggtgt acaatggtta ttttttattt
     1201 cattcttatt ctgataatta tagggatcag ttaccatttt ctttcttatt tttatgcaaa
     1261 gaaattagta agagcgggag tacagagagg atatgactgg tatgaagaca ctggtcatcg
     1321 tttgatggac cctgctgcag tcacgtcagt agaaaaaaaa cgacaaaaat tagaagaaga
     1381 gtgtgaaagc ttttgggagc agggaacacc caaaacaatc aaaagttatg acgggttgaa
     1441 actagctggt cagatgttca tccatccaaa taagcagaat aaatgggtca tttgcgtaca
     1501 tgattaccga agcacaggga aaagagacat gtctcatata ggtaaaagat atgcagagaa
     1561 aggattcaac gttcttgtac ctgatttacg tgcgcacgga gaaagtgaag gagaaatcat
     1621 cggaatggga tggctagatc ggctagattt aattgcctgg atccaactca tcttagatga
     1681 acagccgaat gcgtccatta ttttacacgg cggatcaatg ggagcttcta cgatcatgat
     1741 ggcaagtggt gaaaaacttc catcagcggt caaaggattt attttagaca gcggatatgt
     1801 ttctgtttat gcagaattcc gatatatgtt aagtaaaatc actgtctttc ctaaaaaaat
     1861 ggtcatgaga tatgcgaatc attatgccca gaaatacgcc ggttactcct taaagcaagc
     1921 atcagctacg agacagcttg gcagcaacca tctgccttta ttagtcatcc atggtgaaag
     1981 agatcatttt gtccctacag aagcagcgta cacgattcaa aatgctacgg caggggataa
     2041 agcactgctg cttgttccag aagcagaaca cttggaagca tcgatgaaag atccgaaaac
     2101 ctattggaca gtgatttttt catttattga acaaagaata acgatggatt aattttctct
     2161 tcttttttgt aagtaattcg aggataaata attgtttata gatctggaga ctgctaaaat
     2221 aaggtaggaa taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagaag
     2281 aaaaaaatcg tcttgcttta ttcaatgatg agcaacaaga gattggagaa atgacatggt
     2341 cagatgcggg cccagacatc atgatcattg accatacatt tgtagatcca gcatatcgtg
     2401 gtcaaaagtt agctgaaaag ctagtgtata ctggagtaga attagcaaga cgagacggga
     2461 aaaagatcat tcctttatgc ccttatgcaa aaaaagagtt tgaaaagaaa ccagaatatc
     2521 aagacgtctt acgtcagtca taagaataac agctgaaagt tttgaacttt cagctgttat
     2581 tccatttata tagagttgtc acggactttt atctatgata aagttaaaga gacgatatct
     2641 tttgggaagg aggctgcgat atgtataatt taattttggg aatcgtgatc gttttatcga
     2701 ttgttatggt cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat
     2761 ttaccggcgg tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca
     2821 tgcagcgatc aactgcagtt atgggagcta tctggatgat cttattattt gtcctagcat
     2881 atttatcttc gaagtagttg aataagcctc tcgcaaaacg gcgggcggct ttttttattg
     2941 caaagagaac gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg
     3001 tgagacgagt gagacaatta ccaaaacccc tttatgcaaa acatggaaag cgagcggttt
     3061 tgctgcttca cgcttactca ggaagtccaa acgatgttcg aatgctcgca cgatttttgg
     3121 aaaaagcaga ttatacagtt tatgcgccgc tttttaaagg acacggaaca atggagccat
     3181 atgatatctt gcaagaaaaa gcagaaagct ggtgggcaga cactaaaaaa gccatccatt
     3241 ttttacagtc tgagcagttt tctgatattg ccgtattagg attatcaatg ggcggcatct
     3301 ttgctgtacg agcactagaa gaagaatcag tgattggcgg cggttttttc tgttcgcctt
     3361 tgtctcctgt aaaaacaaac gtgcctgaaa actttgaaaa atacgtacgt caagtgctga
     3421 agacagcagg taaatcagag aaagaaatca atgaaaaagc agttgcttac cgctcgttag
     3481 ctgaacaaca attaatggat atacaagatc aggcagcaat cgtagaaagt aggttgtcag
     3541 atatccaaca gccgatattt ttagctcaag ctgggaaaga tgaaatgatc gaccctaatg
     3601 gcgtatttga aactgcacgc aagttgagcc gtcagcgagt gacacttcaa tggtatcctg
     3661 aaagtggtca tgtgattaca gttggtacag caagacgcga attagaaaaa gacgtcttgg
     3721 aatttttaga aaaattacct tggaatgagg aataaatatg acaaaagaaa cgataaaaga
     3781 gaaaattctc tttttcatgg aaagcagtcg caaacgcagt ttttctatgg aagaaatcgc
     3841 tgaaggttta gggttccaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca
     3901 aatggaaaga gaacagagta tagttttcaa taaaaaagga aaagtgaaac ttcctttaaa
     3961 accaatcttg atcgaaggaa catttcgagc aaatgaacga ggatttggat ttgtaacgat
     4021 tgatcccgaa gaagatgaca tctatatccc aaaagaagcg actggttttg caatggatgg
     4081 agacaccgta gcaatcgata tcatgaagcg agcaaataca gatatggatc gcggagcaga
     4141 ggggaaagta gtcgagattc gagaccgggc gactactcag ttagcagggg aattcgttgc
     4201 ttattcagaa gaagaaatac aagaaacaga tctttacggc gttgttatcc cgaaagatag
     4261 aaagctcaac caatttaaag tatatgcagc agcagaggga gttcgtccag ttgacggaag
     4321 tattgtgctg atcgaactga cccattaccc tgaaaaaggg tatgcgacta gtttagaagg
     4381 gataatcaag caagtgattg gtcataagaa tgaccctggc atggatatcc tctcaattgt
     4441 cgtggctaat ggtatcccta cgaaatttcc tgatgatgta ttgtctgaag cagatgaagt
     4501 tccagatcaa atcagcgaaa aggatataac tggacgtaga gatttaagaa atcagctcat
     4561 tgttacgatt gatggagaag atgcaaaaga tctagatgat gcagttacgg ttaaaaaatt
     4621 agataacggt aattattttc ttggagtaca cattgcagat gtttcttact atgtgacaca
     4681 aggcagtcaa ttggacagag aagcatatga aagagggaca agtgtatatt tgactgatcg
     4741 agtgattccg atgatcccgc aacgtttatc gaatggtatc tgttcactga atccccatgt
     4801 tccaaggctg accatgagtt gcgaaatgga gattgatcca aatggacaag tagtctctca
     4861 tgatattttc ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca
     4921 aatcttagaa gatcaagacg aagaaacgat gcaacgctat gctcagctcg tacctatgtt
     4981 ccaagaaatg ggtgaattgc atcgaatcct tgaaacgatg cgaatcaaac gtggcgcgat
     5041 ttcttttgag gatcgcgaag cgaaaatcct tgttgatgct gaaggacacc caaatgacat
     5101 ccagctaaga agcagaggaa ttggtgaacg cttgatcgaa tctttcatgc ttgcggccaa
     5161 tgaaacggta gctgaacatt ataataaatt aaatctgccg tttatttacc gaatccatga
     5221 acagccaaaa gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct
     5281 ggtccgagga acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt
     5341 agaagacaaa ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc
     5401 acgctattca gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt
     5461 tacatctcct attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag
     5521 tgaagataaa agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga
     5581 tcatagttct aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa
     5641 gaaagcagaa ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt
     5701 tgtgaaattc ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa
     5761 tgaattgaaa caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag
     5821 aactggtctg acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc
     5881 agatgaacga gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg
     5941 tccaaaagca cgcaatggaa aatttggaaa acgagaagga aaacaagcag gcaaacgctc
     6001 agaaaaaaat ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa
     6061 aaaagggaaa aagccatttt acaaagatgt caaaaagaag aaaaagaaaa aataaaatga
     6121 gttggaggaa acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc
     6181 tcgccatgat tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga
     6241 gatcaagtcg atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa
     6301 cggggaagct ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa
     6361 tcatgatccg ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga
     6421 aaacgaattg aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg
     6481 ctatgccaag gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga
     6541 tttgaaacga aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata
     6601 atttcattat cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt
     6661 tagcatttaa tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt
     6721 gcatggggaa aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa
     6781 aatgttttga attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga
     6841 aaaaaccaga tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg
     6901 gattggatga acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt
     6961 gtataatggt gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa
     7021 atcttaagtt gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc
     7081 gaggaatcgt aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat
     7141 ttacattgtt tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct
     7201 tacctaacga aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt
     7261 tggcatttat aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag
     7321 gatatttcgt aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg
     7381 aagaatttac aaatttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg
     7441 aagttttgct aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat
     7501 tagcaattcc attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag
     7561 catttgtctt tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat
     7621 aatactcggt taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat
     7681 tatatcgcag cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt
     7741 caagttatct ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc
     7801 cgtacaacaa tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt
     7861 atcgccttga ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc
     7921 gtttgttact gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct
     7981 atgctgaatc aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt
     8041 gtcagcggct catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc
     8101 agcgatattt tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa
     8161 tctcgcatca actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt
     8221 tctgatgcag ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat
     8281 attttgaagg atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc
     8341 actgtagaac gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt
     8401 caaatcgcgg aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc
     8461 aatcaataca ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag
     8521 acgttacggg aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta
     8581 tcaagatctc gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt
     8641 aagtgatgtt cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg
     8701 ttatgatggg atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga
     8761 ccttttgttc atgatcgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt
     8821 gggaagtgtc acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa
     8881 tgttgcaaaa acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat
     8941 tattggtggc gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca
     9001 attagaaaaa atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg
     9061 ccatcaaagc agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg
     9121 tgctttcagt cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac
     9181 atggtttaga aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa
     9241 tggcgcaaaa cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca
     9301 ttcgtgaagg agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg
     9361 ccttgatcgg tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg
     9421 ttactgacaa agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg
     9481 ttaacgaacc aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg
     9541 gacaacgtga attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgatcgata
     9601 cgatcatcaa ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag
     9661 attctacagt tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa
     9721 tcgttgtgaa tgccggtgcg tctcaaccag caccattgct ttatatcgca ccatatgctg
     9781 gtactgcaat gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg
     9841 atctttcaaa acaagctgtt gcttatcgtg aactttcctt gctgcttcgc cgtccaccag
     9901 gtcgtgaagc ttatccaggg gatgttttct acttacactc acgcttattg gaacgtgcag
     9961 caaaattaag tgatgattta ggtggcggtt caatgactgc cttaccattt gttgaaacac
    10021 aagctggaga tatctctgct tatatcccaa caaacgttat ttcgatcact gatggacaaa
    10081 tcttcttaga aagcgacttg ttctacgcag gtactcgtcc agcggttgat gccggactat
    10141 ctgtatctcg tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt
    10201 tacgtctaga cttagcaagt taccgtgaat tagaagcatt tacacaattc ggttctgact
    10261 tggatgcagc aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac
    10321 aaaaattgca tgcaccgcta gctgttgaaa aacaagtagt gattttgtat gctttgacac
    10381 atggattctt agatagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg
    10441 aatatctgga taccaatcat tcagatatct ttgaaacaat ccgtacaaca aaagatcttc
    10501 cagaagaaga agcgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa
    10561 aaggaaataa ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg
    10621 agatgaatgg gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg
    10681 agccaaatca cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct
    10741 tcttcacaga agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact
    10801 gctactcagt taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc
    10861 atgttgctca gccgtcctgt taagaaaacg ggttatatcg tcatcactgc agatggcggt
    10921 ttagtcggtg gatataacag ctcgatattg aaacaaacga tgtctatttt agaagaagac
    10981 caccaatcac ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt
    11041 aaagcacgcg gtatcaatct agcatatgag ctgcgcaatc tatctgatca gccaagcttt
    11101 gatgaagtcc gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat
    11161 gaattatacg tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa
    11221 aaaatgctgc caatctcaga cttagatcca gaagaagcaa ctactttcga acaagaatat
    11281 atttttgaac cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt
    11341 ttgatctacg gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca
    11401 atgaaaacag caactgataa tgctgctaat atcattgatg agctgacggt ttcttacaac
    11461 cgtgcaagac aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca
    11521 ctagaatagt attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat
    11581 cggtcccgtt gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc
    11641 attagttgtt tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga
    11701 attaggggac ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg
    11761 aatggaagtc attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg
    11821 tcgcgtattc aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc
    11881 ggaaagaagc gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga
    11941 aattttggaa acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa
    12001 agtcggacta ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca
    12061 taatatcgcc caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg
    12121 tgaaggtaat gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat
    12181 ggtgttcgga caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt
    12241 gacgattgcg gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa
    12301 cattttccgt ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc
    12361 tgcggttggt tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac
    12421 atctacgaaa aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta
    12481 cactgacccg gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg
    12541 taaattaaca gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc
    12601 tttagcacct gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt
    12661 attgcaacga tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc
    12721 cgatcaagaa aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa
    12781 ctttaacgtg gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac
    12841 agtgaaagga ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt
    12901 ccgaagtgtt ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa
    12961 aggggtgccc catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct
    13021 atgatcacca tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc
    13081 ctaaacatgc accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag
    13141 attcagatac ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata
    13201 atgttgtttc aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg
    13261 cggaacgtgc aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata
    13321 cgaacgaatt gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat
    13381 ctaagcactc ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg
    13441 ttatgacaga agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa
    13501 atttttgtga aatctcagct tatttttcga agaagctact tctggaacac cgtatatcgg
    13561 aggttgtgaa atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc
    13621 atattttgcc gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg
    13681 aataagggac tgcgacaaga
//
LOCUS       DACDYJ010000069        13124 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDYJ010000069 DACDYJ010000000
VERSION     DACDYJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.069"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(77..149)
                     /locus_tag="IWM51_002163"
     tRNA            complement(77..149)
                     /locus_tag="IWM51_002163"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IWM51_002164"
     CDS             530..1099
                     /locus_tag="IWM51_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP9971563.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IWM51_002165"
     CDS             1086..1946
                     /locus_tag="IWM51_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9971564.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IWM51_002166"
     CDS             2136..2840
                     /locus_tag="IWM51_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9971565.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IWM51_002167"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IWM51_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP9971566.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IWM51_002168"
     CDS             4677..5993
                     /locus_tag="IWM51_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971567.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IWM51_002169"
     CDS             5994..6863
                     /locus_tag="IWM51_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971568.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IWM51_002170"
     CDS             6918..7727
                     /locus_tag="IWM51_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9971569.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IWM51_002171"
     CDS             complement(7830..8459)
                     /locus_tag="IWM51_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP9971570.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IWM51_002172"
     CDS             8629..9921
                     /locus_tag="IWM51_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9971571.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IWM51_002173"
     CDS             complement(9997..10659)
                     /locus_tag="IWM51_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9971572.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IWM51_002174"
     CDS             10884..11168
                     /locus_tag="IWM51_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP9971573.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IWM51_002175"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IWM51_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP9971574.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4359 a         2262 c         2832 g         3671 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatc
//
LOCUS       DACDYJ010000070        13084 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDYJ010000070 DACDYJ010000000
VERSION     DACDYJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.070"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(78..2198)
                     /locus_tag="IWM51_002176"
     CDS             complement(78..2198)
                     /locus_tag="IWM51_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9971575.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2428..3606
                     /locus_tag="IWM51_002177"
     CDS             2428..3606
                     /locus_tag="IWM51_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP9971576.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3622..4380
                     /gene="aroD"
                     /locus_tag="IWM51_002178"
     CDS             3622..4380
                     /gene="aroD"
                     /locus_tag="IWM51_002178"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP9971577.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4403..4888
                     /gene="ybaK"
                     /locus_tag="IWM51_002179"
     CDS             4403..4888
                     /gene="ybaK"
                     /locus_tag="IWM51_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP9971578.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4959..6212)
                     /locus_tag="IWM51_002180"
     CDS             complement(4959..6212)
                     /locus_tag="IWM51_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9971579.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6328..7677
                     /gene="gdhA"
                     /locus_tag="IWM51_002181"
     CDS             6328..7677
                     /gene="gdhA"
                     /locus_tag="IWM51_002181"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP9971580.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7789..9135
                     /locus_tag="IWM51_002182"
     CDS             7789..9135
                     /locus_tag="IWM51_002182"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP9971581.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9443..9829)
                     /locus_tag="IWM51_002183"
     CDS             complement(9443..9829)
                     /locus_tag="IWM51_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9971582.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            10708..10920
                     /locus_tag="IWM51_002184"
     CDS             10708..10920
                     /locus_tag="IWM51_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971583.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11198..12997)
                     /locus_tag="IWM51_002185"
     CDS             complement(11198..12997)
                     /locus_tag="IWM51_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9971584.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2353 c         2577 g         3863 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gcctaatttt gtcgctgctc
       61 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      121 gtttgcgttt tataaagatc aacagttgat tgtcccttgt gttcactaaa gacactagtt
      181 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      241 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      301 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      361 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      421 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      481 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      541 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      601 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      661 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      721 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      781 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
      841 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
      901 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
      961 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1021 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1081 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1141 ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1201 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1261 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1321 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt caagctggag accattctca
     1381 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1441 ttttttcctt tagcgagccc gaacaaatca tcatttggtg ctgcataatg ttcttttaca
     1501 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1561 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1621 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1681 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1741 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1801 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     1861 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     1921 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     1981 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2041 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2101 aaataggcaa aaatcgtttt caaccaaagt aaaacaacta gtaaagaaaa aaatccgagc
     2161 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2221 cttaatcaca gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta
     2281 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2341 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2401 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2461 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2521 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2581 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2641 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2701 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2761 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     2821 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag
     2881 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     2941 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3001 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3061 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3121 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3181 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3241 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3301 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3361 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3421 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3481 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3541 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3601 cattagaagg agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga
     3661 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3721 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3781 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     3841 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     3901 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     3961 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4021 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4081 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4141 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4201 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc
     4261 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4321 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4381 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4441 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4501 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcata tatttaaaac
     4561 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4621 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4681 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4741 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4801 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     4861 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     4921 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     4981 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5041 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5101 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5161 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5221 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5281 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5341 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5401 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5461 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5521 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5581 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5641 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5701 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5761 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     5821 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     5881 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
     5941 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6001 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6061 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6121 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6181 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6241 tctgaagaaa gaaaacagaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
     6301 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
     6361 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
     6421 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
     6481 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
     6541 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
     6601 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
     6661 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
     6721 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
     6781 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
     6841 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
     6901 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
     6961 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
     7021 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
     7081 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
     7141 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
     7201 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
     7261 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
     7321 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
     7381 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
     7441 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgcccagcga
     7501 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
     7561 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
     7621 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
     7681 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
     7741 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
     7801 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
     7861 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
     7921 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
     7981 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
     8041 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
     8101 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
     8161 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
     8221 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
     8281 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
     8341 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
     8401 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
     8461 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
     8521 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
     8581 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
     8641 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
     8701 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
     8761 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
     8821 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
     8881 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
     8941 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
     9001 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
     9061 aagaaaaaca tgttcgcact tctaggcaaa ccgggctttg aagatcttgc taaagaattg
     9121 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
     9181 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
     9241 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
     9301 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
     9361 gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca cttcgagtgc
     9421 ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca gatgagggta
     9481 ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct ttcaactgca
     9541 agatcgttcc ttcggtgtaa gcagaaccat tggcagagtc accagaagta atgacatcga
     9601 aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat ttaaaacctc
     9661 ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt ggagctttga
     9721 ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa cctttccatc
     9781 ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg aaagatctcc
     9841 ttttttattt ttattatatt aaatattata cagataataa cggaatatta tcatcgaaac
     9901 agctaacaaa attgtcataa aaaacgagat ggttgatatt agaacttgtg tttttgttta
     9961 ggtctagaga taaagagaga caatttccaa atatatgtaa tgaatacaaa aagacgtttg
    10021 tcgcggatta ccgttacaaa cgtcttttat tgattttccg agatctcatc agtcgccatt
    10081 tggaatggtt cactccttat ctagattcgt ttaatgctcg gactgcttgt tcgattgtaa
    10141 taccaaacgc aacgttattt tcgttttgtg catcaatcgc cataaaacgg ttagaagttg
    10201 tatcaaaagc tacgcggtat tgcatatttg tttttgtaaa cgtcataaaa ttcatctcct
    10261 ttgtaatgta atttagttct tgaaagtttc ctttaaactt tcgaacttat accaagttta
    10321 gcatcgtacg ttttttgatg caattaaaaa gcatttatca ttttccttat ttattttttc
    10381 ttaaatggtt tagctatttt cttttaatcc gataagtagc ccaatcatca aggttacaaa
    10441 tactattgct tgaatataaa tcatatgctc acctctttat aaataactta gcaatataaa
    10501 gaaaaaaacg cacgaacaac ataggaatat aaacaaacgt ttttgtcaca acgtctaaga
    10561 tcgtgttaaa aaatagttat ctaaaaagca gaaagatact caaaataaaa aataaaaaaa
    10621 tgaataaaaa cgttttcaaa tacaaggtaa tttgttatta taaaataaag aaaaaacaaa
    10681 ataaaaataa agaaaagagg gaatgaaatg gtaaaagcag aagtaggaag atcttatata
    10741 tgcaaaccga tcggagtatc tcatgaacta atcggctgta ttgaacgtac atatatcaac
    10801 acggctgtcg tgtatgttga aagttatgac caacgagact atcaacttat caatgaaagc
    10861 aagtatcgca tgctcgtaaa attctgtgat attagcgcac ctactgaatt ggtttcttaa
    10921 acagtatttt tgagaattcg caagaactcc cttataatat aaataaagac agaagggagc
    10981 gagaagatat gccaaaaaga aaaagttcag ggaaaatggg aattattctt gcagtgatct
    11041 ctgcaattcc tgctatttta gctattgttc gtgcgatgaa ggaatcaaga gaaagttaag
    11101 cacacattat gagcgtcaaa caaaaaacgc atatacctgc ggtggcaggt tatgcgtttt
    11161 ttgttgtata aagacttttt tcattaacga tttttgatta gccgactccc aaaacaaaat
    11221 ttagcagttt atctgaataa gtgatttctt catctgtttg tttcacactt tcatttagta
    11281 gtcccatctc atcggttatg ataccatcca caccataaaa catcatgcga ctcatggcat
    11341 ctgaatcgtt gattgtccat acatatactt cttttccgtc ttggtgggca gaattaatga
    11401 agttacggtt gatcgtacta tattccattg tcagaaaatc agcaggcgtc acagggggcc
    11461 caacaatatt gaaaggtaag atgtagccca cataaaagtt tggttctgct tcttttaaat
    11521 ctgaaacggt ttggtacgtc aaactttgca aaatatgatg gttctctaag ataactttgc
    11581 ggtatttatg gacaaatcgt tcgacaaggt catccgaatc ttttttagag gtcttgatct
    11641 caataagtaa tttttggttt agcttatgtg ctttttccaa ataagaatca aaagaacaaa
    11701 caggggcttg cactccgttt tcagtaactg taagatttgt caattctttc aatgtaagat
    11761 cctgtggtgt tccatttacc cctgttaagt gacgaagatt tgcatcatgc atcactacga
    11821 aatgaccatc ttttgtttcc tgaacatcca tctctacata atcaggatga tagtctctgc
    11881 ttgttttttc aagtgcttct aaagtatttt gcgccccatt ttgggtactc acacctctgt
    11941 gagagacagt cactggtttt gtctgaacag ctgaagtcag ataatccata ttgtaaagac
    12001 agacgccaat accgaaaaaa actgcaaaaa gagtcaaacc agtgtttttc agcgcagaga
    12061 aacgcaagtt gggggcttgt gggataaacc acttagggat ctcaggcaag aagccttcgt
    12121 catccatgaa atcaacaatg atataaaaaa tccctacggt agacatcacg atattgaata
    12181 ataaaatccc ttgtaaaagc gtcatagcaa acacagcgct gatcaatgca tagtctggga
    12241 aaaattgttc aaccatactt tgtgccaaaa taacaacgat atatcctaaa gaagaaagga
    12301 gtaaaatcgt accgccaatc acaataaatt ggcccgtgat ggccaaaaga cgcgatttgg
    12361 tcaatgacca gctttcccga atggctgctc gaaaagaacg atctcgtaga atcatttccg
    12421 gtaaagcaaa aatcagtcga atcccaatat agccaaggaa aatataaacg agtaaaaagg
    12481 aactgacgat gatccaacga ttagcaaaaa taaaatccat aatgaaagcg gggattttta
    12541 tttttgacag tagatcagaa ttaaaactta atccgctgat cggtagaatc aataaaaaat
    12601 aagcaaggaa aaacaaaaaa gtaatgggtc tcactttctt cagctgaagt atcgtcaagt
    12661 gtaagagttg cttcaaagag attggttctt tctttttaat aaaaaaaaca ctcatcaaaa
    12721 gaaaagtaaa ttcgaaataa actaacagta aaatcgtcat caaaactacg attaacgaga
    12781 gtagaacccc aggatgttga gaaaaaattg aaggaatatt gtcataagac aaatagtcaa
    12841 ttgcccctcg gtgtaaaata aaacgggtcg tactgctcaa taagggtaaa caaataaata
    12901 aaataaagcc atgcatcagc aagacatcac ggaaataagc ttgtgttcct tttaaaaagt
    12961 cccaagcatt acctaagcta cgtttcaaat aattcatcct atcacctgct cttcacattg
    13021 tagcgcaaat ctaatgaaaa aggaaagggt gcagtgtgtt ttgcagaccc tttcccagat
    13081 ttaa
//
LOCUS       DACDYJ010000071        12936 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDYJ010000071 DACDYJ010000000
VERSION     DACDYJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.071"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..767
                     /locus_tag="IWM51_002186"
     CDS             <1..767
                     /locus_tag="IWM51_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971585.1"
                     /translation="IKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWR
                     LNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTL
                     IAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELD
                     NSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKL
                     AIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(912..1652)
                     /gene="truA"
                     /locus_tag="IWM51_002187"
     CDS             complement(912..1652)
                     /gene="truA"
                     /locus_tag="IWM51_002187"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9971586.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1843..2478)
                     /locus_tag="IWM51_002188"
     CDS             complement(1843..2478)
                     /locus_tag="IWM51_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9971587.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(2625..3866)
                     /gene="sstT"
                     /locus_tag="IWM51_002189"
     CDS             complement(2625..3866)
                     /gene="sstT"
                     /locus_tag="IWM51_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP9971588.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4064..5062
                     /locus_tag="IWM51_002190"
     CDS             4064..5062
                     /locus_tag="IWM51_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971589.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            5075..7093
                     /locus_tag="IWM51_002191"
     CDS             5075..7093
                     /locus_tag="IWM51_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP9971590.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(7153..7869)
                     /locus_tag="IWM51_002192"
                     /pseudo
     CDS             complement(7153..7869)
                     /locus_tag="IWM51_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
     gene            complement(8096..8638)
                     /locus_tag="IWM51_002193"
     CDS             complement(8096..8638)
                     /locus_tag="IWM51_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9971591.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(8643..8870)
                     /locus_tag="IWM51_002194"
     CDS             complement(8643..8870)
                     /locus_tag="IWM51_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP9971592.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8949..10829)
                     /locus_tag="IWM51_002195"
     CDS             complement(8949..10829)
                     /locus_tag="IWM51_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9971593.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11018..11632)
                     /locus_tag="IWM51_002196"
     CDS             complement(11018..11632)
                     /locus_tag="IWM51_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9971594.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            11774..12334
                     /locus_tag="IWM51_002197"
     CDS             11774..12334
                     /locus_tag="IWM51_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9971595.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(12654..>12936)
                     /locus_tag="IWM51_002198"
     CDS             complement(12654..>12936)
                     /locus_tag="IWM51_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP9971596.1"
                     /translation="EDIFVIKRRYFTQVSDDLIHVFSNEWFVSEKKLHASNLQYMPGE
                     DPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPL"
BASE COUNT         4061 a         2544 c         2049 g         4282 t
ORIGIN      
        1 ttataaaaaa attaactgct gatgatttat ttattttctt tcttggtgta tttgttcttt
       61 tttgttcaac caatttattt ccttggcaac tttttaacca tacaccagtt aagagtctac
      121 aatttgtctg gcggttaaat ggtttttctt ctttatttat agcctatacg atgagtatag
      181 tgatctatta tattttttct actaaaaata attcatggaa aataatggtc tttacgtttc
      241 ttgcactaat attgcatctc tcaggagtaa gcaattctat ccatgctaac aaagattcat
      301 taacacttat agcacctgaa gacgctgttg ctatagctga aaattacaat catactgatt
      361 atgcaaacaa agagtcaatc tatcatcctg atatgattaa taatgatcaa tatttactag
      421 gtaaccagga aataaatcca acaactcatt ttatgagtaa caaacttaca attgaattag
      481 ataattcgaa taataaaaat actgtcttga cgacacctat ttatcgttat aaagggcaag
      541 tggcttctat taatggcaaa ctagtacaaa caaaattatc taagtttggc acaactgaat
      601 taactattcc tccaggtata aataaagtcg ttattactta tcagtacaca aaactagcaa
      661 ttgcttcaag atatttatct atcgttactt tgattttatt tttactttat cgatttactt
      721 tttctaaaca aaaacaaccg agaagagaag tacaacattc tcactagatt tttatttaag
      781 aaagactttt tagctgtaca cataaacagg ctaaaaagtc tttttttata tttctttaat
      841 ttgcttcata cattatttaa catcgatatt tatactgtga aacatgcgtt tcatccatta
      901 tcaaagtatt tttatcgata ataaacttca tccaaaaaca gaccttgtgc agggacggtt
      961 tctcctgcat gttgccgcac tttgctttca aatatttcat cgattgtttc tatcggcatg
     1021 gcacctgtgc cgatttctag taatgtcccc atgataatcc gcaccatttt atataagaat
     1081 ccgtctccga caaaagtaaa atgaagcatt tctccctttc gttcgataga taattcatca
     1141 atcgtacgca ctgtggattt ttttgatttt ttcaatgcag aaaaaccaat gaaatcatgt
     1201 gtacccacta atttttcgca agctgcattc agcttgtcca tatccaactc ttgagggaaa
     1261 taaaagctgt ggtttcgctc aaatgctgaa ggaatcacgt tgttccaaac ataatagctg
     1321 tattttttgc ccaccgcatt atatcttgaa tggaagcgct caggcatttc ttcgattctt
     1381 ttgatgacaa tatcccgcgg aaggtaacga ttcataaagt cgatcatttc tgccagtcca
     1441 atttctgtgt ttgttttaaa atttgctact tgtcctcgcg catgggtgcc agcatctgtt
     1501 cttcctgatc caataatttc tatctcttca tttgtcatct gatgcaatac tttttcgatt
     1561 tttccttgaa tggttttatc gtcatctcca agtctttgcc atcctgaata tctttttccg
     1621 tcatattcga ttgtcaactt gatgtttctc atattttagt ggctcctctg tcttccattt
     1681 ggtcttctct ctaccttttt aagattgtag cacaaaatat agctccttgc ttctctaaga
     1741 cagaagctat acaagaaaac tgtggaaata tatatttcag aaaaacaaaa aacagccacc
     1801 ggatgaagta tccgctgact gttcaatttg acggctcaag ttttactgtt tgagatgcaa
     1861 gatactcaac caggcaatgg gcacataatt aaagaaaaaa ctgattcttt ggcaaaaacc
     1921 tattttattc agtaatggcc aagaatttat agaaggaatt cttgccagtc cataaatcgc
     1981 cgcaaaaagc aaactgatca cagacagtcc aaggtataag tggcttacgt ccatttgccc
     2041 attttttcga tagatcaaga aaagaagaac tggaaacaat aaaaaaccac tgtaacctaa
     2101 acctgatccg atattatgga cccatgtcga gacattccac gaagattcat gggtattgat
     2161 actgaacaaa cctgtaaaga tacaatcacc tataccatag aacgcgatgg ctgctgtaac
     2221 tagggtagct aacacacgag atgtactaac aaatgcagca tacaatgcag gcaatgacag
     2281 gatgaaaaag atccctgaaa ccacagacca aataagaaac gcttttctta caggactgga
     2341 aacctctcca aatacactga tcacgctctt catttgatct aattcaggat aatacaatcc
     2401 taatacgtac ggagtcaaaa aatcactcag cacgcctaat aataaaaaga aaaaaccaaa
     2461 tttacgtaaa aaatccatga tacgcctcca ataacaatta cctatgcttt taattgttat
     2521 tataaacgac tttatcttga tttaacagaa acctctattt acacgaacat ctatataact
     2581 cattaaaaac tcgaatcatt ttaaaaatac taaatatcta acgttcaacg ttcacttaat
     2641 ccatctaaac aatctttcaa tttgattttt tccccacttc ttttacgcgc gcctagctct
     2701 cctattgcag taaacaacag atcacttgac gaatttaagg cagtttccat tgaatcctgg
     2761 ataacaccga taataaatcc aataccgacg atttgcatcg cgacatcatt agagatacca
     2821 aacaaactac atgctaatgg aatcaataac agtgaaccgc ccgcaacacc tgaagctcca
     2881 caagcagcta aagcagacaa aatacataaa aggactttca aaacaaaagg tgcttcaatc
     2941 cctaaagaat gtacagcagt caacgtcatc aaggaaatgg taatcgcggc accgcccata
     3001 ttgatcgtag cacctaacgg gatcgatatg gaataagact cttttgtcaa tttcaatctt
     3061 tcagccaaca tcatgtttat cgggatattt gcggcagaac ttcgggtaaa gaaagcggga
     3121 agtgcgcttt ctttcaaaca aaagaaaatc aatggatagg gattttcttt aataaacaaa
     3181 aacgtgagga atgggtacac aactaatgcg acaaacagca tcgtaccgat caaaacaagt
     3241 aaaagctgca gatactctgc caatccagcg attccagtcg tagcaattga ttgataaacc
     3301 aatcctaaaa tccctatcgg agcaaatgca ataatcactt gtacgacttg tgacaacgct
     3361 atagatatct gatccaccaa ctcttttgtc gattctgaac gtgctcgaaa agctagtccc
     3421 aacaatacag cccagaataa gacgccaagg taattcgcat caataagaga ttgaacagga
     3481 ttttgtgtga cattcgtcaa catagtagat aaaacacttt ccaaactttg cggtgctttt
     3541 tcagatactg tttcttgtaa agtaatagga acagtaaaaa ttgaacttac aataactgct
     3601 acaagtgcag aaagcaatgt tcccactaaa tataagatca aaataggctt cacgaatgtc
     3661 ttattcccaa ttttatgctt ggcaattgac gccatcgtca agaaaaaaac cagaataggt
     3721 gcgattgcct tcaagcaact gacaaacaac gttcccaata ttgaaataaa tgtccatgtg
     3781 gggaaaaaga taccaaacaa tattccgact aaaataccaa caacaatacg ttgaactaac
     3841 gacatatcca aaaacttctc taacaaatga ccaccctttc acaattttaa caatcattgt
     3901 atcattttat gcaaagaaac taaaggtata attttctaaa aattctaaca atacaaacaa
     3961 ccatttcttg ctaattttgt actttttctg tctacacgtt gttttttgag atattctaag
     4021 ttaggatgtt attaatgggc aacttatcag gaaacgtttt cttatgtttt tgtccaattt
     4081 ttttgtgagg tgtttattct tgaaaaaaat aaagaaggag agttatggct tcctgacaat
     4141 ctttgtcgct tgtataatga tggttttaca gcgtatattg atttcagcag tcactattcc
     4201 tacatatgca acaaaccctg gtcgattcta catctataca tttttacaaa cttccgttgt
     4261 tattggtgca aatttgatcc cactttacat gggttaccaa tatcaaaaaa tcgcccgttt
     4321 aaaagtttta cattatttaa gtcgttttgc ctttatattc ctaatagcca cactagtttg
     4381 caatatcttt ttttacgtac aagctggcag cttgaatatt cgtgattatt ggcttatcct
     4441 aacacctatc agtcagaatt actttactta tgctgtttct tgtactttgc ttttttgtag
     4501 tattccttat acagttggat ttttaaataa attatcgaaa acaacaatta aatatctact
     4561 attattttta acaattggat tagttggctg ctctactcta tttaataaag acccgtttgc
     4621 attacagaat ggacagagta ttttatggat tttttattta tttttaatgg gttatggttt
     4681 gaaagaatgg aactggaaag aaaaggtccg ctataagctt cctcaagtat ttatcagctt
     4741 gattgtttta ttcggtttga ttattttgat gacacaaatt tcactcatca tcagaggaaa
     4801 tacagataca gcgctgaggt tttctaaacc atattctctc ttcgcaatgt attatacttt
     4861 ctcttcgttt ttatttttag aaatgctatc tgataaatta ggtataaaag gaagaggaaa
     4921 ttttcttgct gtatttttga ttgtcactca agttatatgt aatttccctt tagtttctta
     4981 tcgcgtatct acatttatga aacgtgaatt tccaaactca gggaaggcat ggctgataaa
     5041 tatcgggcag ttttttattt gatatttatt agcagtgtcc ctcctagtta tctttttatt
     5101 ttatatacaa aaaatccgtt tcattcatcg tttcatcgaa tacttcgctt ttgattcgat
     5161 tgaacaatta actcagcgtt ggaaaaaaag aattcattgg ctttttgttc ataaaagtta
     5221 ttttttagtt gcagcatttt tttactgctt taccttcgtg cagattttcg ttttagaacc
     5281 aaaagaaggt tggaaagaaa ctatccaggt tgccttaaaa atatttactc agcgacaagc
     5341 acccatgatc ctgacaatca tcattattat gggatttttc ttcttactat tgttgatttc
     5401 caatcgtttt tggtacgcct tgactgctac cttgatcatt aacttactgc tcactatttc
     5461 gacagtaatc aaaatggaaa tgcgggaaga gcctgtcttt ccttcagatt taaaaatgct
     5521 gaccggattg tcagaactat tatcgatggt tagcccagtc ttattaatcg tgggcggatt
     5581 gatccttctc ctattattga ttactagtat tattgtacaa cgtagactac aaaaacagta
     5641 ttccttgaag attcattgga aacgtcgatt tatcagtatc gctgtattat taggatgttt
     5701 ttcgggtgtc tttttcatta atcacaaaaa ttcgccttct ttcttgctgt ttaatttatt
     5761 taaagtaaac aagactttct ttaaccagca aggcgccgta aaagataacg gaccagtcat
     5821 tcaattccta aacaatatcg acattaaaat catggaagaa cctgctggct attcgaaaga
     5881 aaaaattaat ggaataatga aaaagtacaa cacaaaggca gatgaaatca accaaacaag
     5941 aagtgactgg ttaaacaatc agacgatcgt tctcaattta agtgaaagct tcagtgatcc
     6001 tgcacgtgtc cctaatctga cagtacctac agaccctatt ccttacatca aaagcattat
     6061 aacagaagga acaggaggat tgatgttatc cgtcggttat ggtggcggaa cagctaacat
     6121 ggaatgggaa ggactgacag gcttgagtat cagtaacctg tctgcttcgt tagttacacc
     6181 ttatacccag ctagtggagc gtcagaaaat aagtccaaat attaccgatc tattcgatga
     6241 aaaaatagcc atccatcctt ttacagctgc tctttatcgc agaaaacaag tgtttgataa
     6301 atttggtttt gacaggttct attatttaga cagtccggat aaattaactt atacggataa
     6361 aatagaacaa agcccacgca tatcggatga ttcagcatac aaagaaacct tgaaagcttt
     6421 acaaagcaat acggaaacgt cacaattcat ccaattatcc actatgcaaa accatatgcc
     6481 ttatgatgat tactatagtg aattgaacta cactgctgaa ggaactgctg tcattcctaa
     6541 tagaagaaaa gaattacaaa cttatatgca aggccttcac tataccgata cagcagtaaa
     6601 aaaattcatc caagaaatcg acaaaatcga taaaccgatt acctttgttt tttatggtga
     6661 ccatttaccc gcaatctata gtgggaatga ttcgaaaaaa tatggattag aacagcacca
     6721 gacagattat ttcatttata acaacaagta tagtcgtgaa caagcagcta cgttaaataa
     6781 agaagttgtt gctcctaata actttggtgc tatggctctt gagcatgcga atatcaaagt
     6841 aactccttac tatgcgttgt tgacagaagt atctaatcat ctacctgcat taacaattga
     6901 tcctacagaa agcttgtcta atcgttttaa tggaaaacag gtctttgtta cagaggatag
     6961 tcaaattatc caagaaaaaa atttaactga gaaacaaaaa gaaattctag aagattatcg
     7021 tctgatacaa tatgatcttg ttgcaggtaa ccagtacagt gcaacatggg cgacacaaaa
     7081 aataaaagac taacaaataa actgagcaag gggttgtgat aaaagacatg tcacaacccc
     7141 ttgtttatct cattaatttg cttgtccata aacgatttga ttaaagccat ttttcaattc
     7201 attattcgct gtctcgttga agtaaagata attatcggga ttcccctgat tttgttgtgt
     7261 caccaatact tttggctgat tttgctgaaa cccgaataga tagacgtgct ttttcagtta
     7321 tggttctgtc tctgcatcgg aatagacggc tgtgatttga tagtctgctt gacctgtccc
     7381 actttctgac cattctgcag aaacaggcac tccaccaata gccatcttcc actccccgtt
     7441 caaaatagct tgagggactt taagaccata taagtccaca ctcatttgat tcgtataact
     7501 tttgtattgt tgtcccattg tttggcccca tttggtcata aatgcctcta attgtgcgga
     7561 tttgtctgca ttccacagtg tttgttcaac ttgttcgctg ctttctgaag aggaagatga
     7621 agcctctaca gaagaacttg aagtagatgt ttgagttgtt tcagtcgttt tggaagactc
     7681 tgacgaagta gaggtatctg ctgtttctgt acttgctgca gttgacttgc tctctgtgct
     7741 agttgttgta gtagttgcaa ctgttgcttt cgctgtttct tttgttgatt cttttttcga
     7801 ttcattcccg ttggtacaac cagctaacaa aaaagctaga ccaattgtta ctaaaatcct
     7861 ttttttcaat taaaaaactc ctttcgatta ctcccacttc tttattctat caaataagca
     7921 atgtgatttt attgattttt cgtattttct catcaatctt tcaaatcttc taaaaactga
     7981 gaagcacccg ctgatgttgt ctgtaataca tcagcgggtg cttcttttat tttaatcttc
     8041 gtgattttcc aatttacctc acgctttata tttgtatttg aagaaacata gacttttagt
     8101 catcatcgtc ttcctcatcc aaatcttgtc ggacagtttt tccttggagt ctttgtactt
     8161 ggtagatgat ttctttattg aaacttaaaa gttcaacaag caattgttgc aagccatatt
     8221 tttcttcttt ttcagctaat ttgattgccc gtgtgataaa gacattttgg aaatcaaaat
     8281 cttttgcaaa gttttccaac attgttgagg ctggtaaata tttgttttga ccatgttcgt
     8341 aaatttttcc ataacgcatg acttcttcta aagtagacgg aacgatttca ttttcaatca
     8401 ctaactcatg agagatttta ttgaaccatc tcgcttcttg atcagcatat tctgaaaaaa
     8461 gggtacttaa ttcttgcgaa gatgtaccca aaacatacaa ttgtgcttgg atcaatttca
     8521 tttcttctaa tttaaaattt gctaatacat gccccaccat ggcgccagca gttggtttat
     8581 gatggtcgat atctgcttgt tttgtttctt gaacaaattt ttcttccact atattcattg
     8641 atttacgcct cttttacttt tgctgattgg acttcataac ctaatttttc cacaactgca
     8701 gctaattgtt cagggtggac ttcatctgga tcaaagtttg ctttgatttt gctcgcatta
     8761 aacaaaactt tcgtattgct taccccaggc tgttgagaaa ccgcatgttc gattttttgc
     8821 atacagcttg gacaagctag tgtatctaat tgcataataa tttttttcat cgtagttctt
     8881 ccttcctttt tcttgatagt cttattctag attacagctt ccataaaaaa attgattctt
     8941 atcaagtttt acgatttttc ttctttgctt tcgtgtccaa aatacaataa acgcatcgcg
     9001 ttaaagataa cgatcaagat actagcttcg tgaacgaaca tcccactagc catgtaaata
     9061 aacccggcaa ataagccgtt gagtaaaaat aagacgacac caatggcaat cactacattt
     9121 tcacgggtat ttaaaacagt tttcttagcc aagcggtagg catgaaccaa cgattcaaaa
     9181 ttagattgca tcaagacaac gtcactcgtt tcgatagcaa catccgttcc tgatcccata
     9241 gcaatcccga tatcagccgc agccaaagag ggagaatcat tgattccatc tccaataaaa
     9301 gcaacgcgca atccttgttc ttggtatttt ttcacaaatt gaaccttttc gtctggtaat
     9361 aattcagcat ggacttcatc aatgcctaac atatcagcta cataatcagc agtcatctga
     9421 ttatcacctg tcagcatcac taactgtttt gctcctgctt gtttcaactt agctaattgt
     9481 tcagcgactt ctggtcgaat cacatctgac acacctatga tttgagtgat ttttttgtcg
     9541 attgctacaa taatgaccga gctgcctgtt tgttgtaatt gatgtaaatc ttgtacttgc
     9601 tttggtgtga gagaaagttt gtgagcggca atcaatttgg tgttgcccgc acaaactgta
     9661 tgaccatcaa tctcagctgt gattccttgt cccttgattg tctgattatc tttgactgct
     9721 aattgttgaa aatcaatgtc tttctcctga gcataatgga tcacggcgcg ccccagagga
     9781 tgatcggata acgtttctaa tcgagccact aaagctaagc tgtccccttc atcctctgta
     9841 tagttcttga tagtggatac agcagtagtt cctttggtca atgtaccagt tttatcaaag
     9901 acaaaagtat cgattttgga aaaagtatcc attgcttcac cgcctttgac taagatgccc
     9961 cgtttcgctc cattaccgat tcctgctaca ttcgaaacag gcgcaccaat gactaaagcg
    10021 ccaggacaac caagaaccaa aacagtgatt gctaaacgaa aatcacgact aatcagacca
    10081 acaaccaacg caataagtaa aacggttgga gtataatact gagaaaatcg atcaatgaat
    10141 ttttccgcat gggatttggt atcttgtgct tcttctacta attcgataat tttggcaaag
    10201 gtcgtatcat ctccgacctt agttgcttca atcgtcacgt agccatctgc taaaatcgtt
    10261 ccagagaaga cttcatctcc actgattttg acgacttgtc gtgattcacc agtcactgcg
    10321 gcttcatccg catacccttt cccgtcaatg actcgtccgt ccacaggaat agctccacca
    10381 gtttttacta agacgatatc gccttcttcc acgtcatcga tttccacttc ttcagtcgtt
    10441 ccatcttctt ggacaacact agcagtagat ggggccatct cggtcaaagc cttgattgac
    10501 tcccgcgttt tggtcaatgt tttttgttcc agatagctac caaacaaaaa caagaatgta
    10561 acgatagctg actccttgta ttctccgatc ataaaggcac caagcacagc aatagtaacg
    10621 agtaaatcaa ttgagataac tttgactttc actgcctgat acgcatggat cataataggt
    10681 atgaccgcaa taatagaagc tatggctaga gcaatgttat agaacaagac aaaaccagta
    10741 aattttccga taaaaccaaa aataatcaac acagctgata aggcggtgat atgatttcga
    10801 tgctcttgga tccacttttg taatttcatc tttctccctc ttttcctttc attcttacga
    10861 gactaagtat agatacttat ccatcataaa aaattgacca ctatcaagtt tagtcaaaaa
    10921 ggcggttttc ttttggtaga atacactaca aaaaatagtt agcaagaaga ctcactaaaa
    10981 gtgatcttgc caactattgt cgtttctatc gaaattttca ttgaatgatt ttcctgtaca
    11041 gcatacatcg gagatctatt tctttatcta agatttttat cttgctaatt ttttccttta
    11101 gtctttcaaa tccacgtcgt tcatagaatt gatacgtaca gccactatct gtaaaaagga
    11161 atatttcttt tcctttctct cttttttcaa attctgttag caacttggta ccgatgcctt
    11221 ttgttttcaa atcaggattt gcagctaaaa aaatgatctc tccatctggc gaatgcttct
    11281 ttaaatatgc tgcaagcatt tgttcatttg ctgtctcgta agctccttta ctttctttag
    11341 caaatatatt ttgtaaaaaa tcgaatatct taacatatat ttgctgtcca aaagaacgat
    11401 gtttcttttt ttctcctttt atctccgcca gcaaaacacc tgccaactcg tcctctgcat
    11461 acaaagcaag aatttgagtt gctcgattca actccatata caaaaaatat cttccgtaaa
    11521 gattcagcag aagttcattt tccatatatc tctcaaagtt cattccttca acagcaaaat
    11581 agatagcttt tttgtaatct ttcttttcta agggtttaaa agttagtttc atcatttcgc
    11641 ctcctcaact tcaatgattg tgttatactt acattataat agacacagtg tctactatct
    11701 ataatcaatc atctataagt ttgtcgatta agcgacagct ttgctttgat atgtcggata
    11761 aggagatagg taaatgaaaa agacagatag acgcgtcaaa aaaacagaaa aagctttggc
    11821 tgaaacacta tccacattat tagtaaataa aaagattcaa gcaatcacta tacgcgaact
    11881 aacagaaacg gcagatgtac atcgttcgac tttttataca cattataagg atatctatga
    11941 tctatatgat caattagaaa gcaacttttt tcgggatcta aatgaaattc tctcttatga
    12001 tccagcccat agctatgagg aactatacac tcgcttgatt gactatgttt acgcaaatac
    12061 ttttttatat cgcttattta tcctcaacca tggtgatatt tcgtttcaga agaaagttaa
    12121 acaagttatt gaagaaaact atctgaacat atggctatat gaagacaaga aaacgattat
    12181 cacagaagaa atgagattct ttactacgta ccacgtccaa ggttgtctat ccgttctcaa
    12241 tcggtgggta gaaagcaatt acaagtactc caaaaaagaa ttattagaac tcttacagaa
    12301 actgaatgat cacatagaga aaatcatgcc ttgaataaaa acatactaag cttagttaag
    12361 aagaaccgta accttctcgt ataacaactg aatcttattt agccaaattg tttactgaag
    12421 attttttgag taaaatttct ataattccta cttattgaaa agtagtttgt agtgctgctt
    12481 ctccctctct aagtgatata tcttgttgta ccaatcaaat tcacctcatc attaataaat
    12541 tacttttagg ctaatttatt acaacttata ggttgaagct gtaaccaatt tatagtcata
    12601 ttatattttt atcatccagt ttattgaatt cttctcgttt acctcaattc attttaaagg
    12661 ggaatcagct catcatctaa cattttacgc catgctcgct tcatttcttg tgggtattta
    12721 aatggtttcg cttctggatg tttggcttta tatccttcgt aatccttact gttgataatt
    12781 gctttcatat taggaattgg atcttcacct ggcatatatt gcagattaga agcatgtaat
    12841 tttttctcag aaacaaacca ctcattggaa aagacatgaa tcaaatcatc acttacttgt
    12901 gtaaaatagc gacgtttaat gacaaagata tcttca
//
LOCUS       DACDYJ010000072        12670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDYJ010000072 DACDYJ010000000
VERSION     DACDYJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.072"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWM51_002199"
     CDS             6..305
                     /locus_tag="IWM51_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9971597.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWM51_002200"
     CDS             378..1739
                     /locus_tag="IWM51_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9971598.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWM51_002201"
     CDS             1761..2021
                     /locus_tag="IWM51_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971599.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWM51_002202"
     CDS             2035..2787
                     /locus_tag="IWM51_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP9971600.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWM51_002203"
     CDS             complement(2881..3486)
                     /locus_tag="IWM51_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9971601.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWM51_002204"
     CDS             complement(3539..4513)
                     /locus_tag="IWM51_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP9971602.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWM51_002205"
     CDS             complement(4891..5670)
                     /locus_tag="IWM51_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9971603.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWM51_002206"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWM51_002206"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9971604.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWM51_002207"
     CDS             7161..8558
                     /locus_tag="IWM51_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9971605.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWM51_002208"
     CDS             8639..9079
                     /locus_tag="IWM51_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9971606.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IWM51_002209"
     CDS             9227..9553
                     /locus_tag="IWM51_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9971607.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IWM51_002210"
     CDS             9554..10303
                     /locus_tag="IWM51_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971608.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IWM51_002211"
     CDS             complement(10383..10838)
                     /locus_tag="IWM51_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9971609.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IWM51_002212"
     CDS             complement(11265..11540)
                     /locus_tag="IWM51_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971610.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IWM51_002213"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IWM51_002213"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP9971611.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4056 a         2175 c         2587 g         3852 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACDYJ010000073        12226 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDYJ010000073 DACDYJ010000000
VERSION     DACDYJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.073"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(93..746)
                     /locus_tag="IWM51_002214"
     CDS             complement(93..746)
                     /locus_tag="IWM51_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9971612.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1034..1554
                     /locus_tag="IWM51_002215"
                     /pseudo
     CDS             1034..1554
                     /locus_tag="IWM51_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1456..2172)
                     /locus_tag="IWM51_002216"
     CDS             complement(1456..2172)
                     /locus_tag="IWM51_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP9971613.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(2296..2481)
                     /locus_tag="IWM51_002217"
     CDS             complement(2296..2481)
                     /locus_tag="IWM51_002217"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971614.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            2708..3981
                     /locus_tag="IWM51_002218"
                     /pseudo
     CDS             2708..3981
                     /locus_tag="IWM51_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4088..5119)
                     /locus_tag="IWM51_002219"
     CDS             complement(4088..5119)
                     /locus_tag="IWM51_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9971615.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5126..5956)
                     /locus_tag="IWM51_002220"
     CDS             complement(5126..5956)
                     /locus_tag="IWM51_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9971616.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(5953..6759)
                     /locus_tag="IWM51_002221"
     CDS             complement(5953..6759)
                     /locus_tag="IWM51_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9971617.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(6765..7589)
                     /locus_tag="IWM51_002222"
     CDS             complement(6765..7589)
                     /locus_tag="IWM51_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP9971618.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(7579..8679)
                     /locus_tag="IWM51_002223"
     CDS             complement(7579..8679)
                     /locus_tag="IWM51_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9971619.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            8857..9642
                     /locus_tag="IWM51_002224"
     CDS             8857..9642
                     /locus_tag="IWM51_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP9971620.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            9675..10058
                     /locus_tag="IWM51_002225"
     CDS             9675..10058
                     /locus_tag="IWM51_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9971621.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10134..10265
                     /locus_tag="IWM51_002226"
     CDS             10134..10265
                     /locus_tag="IWM51_002226"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9971622.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10234..10470
                     /locus_tag="IWM51_002227"
     CDS             10234..10470
                     /locus_tag="IWM51_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9971623.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(10548..11519)
                     /locus_tag="IWM51_002228"
     CDS             complement(10548..11519)
                     /locus_tag="IWM51_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP9971624.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3682 a         2758 c         2242 g         3544 t
ORIGIN      
        1 gataaaaaaa attaatgaat gaactaatat aattattatt ccaagaacag aaaatatttg
       61 tatgaacatt tttggaaata tctttaattt tttcattttc tctccaattt atatcctaca
      121 tttcttatag tagttataca gtctaattgt aatttttttc taagttcttt tatatataca
      181 tctattactc gatcataagg agtttcttcg ctatctttcc atacataatc aataatttgc
      241 attcttgtta acacttgtcc attattatcc aataaacatt ttaatacttc tagttctttt
      301 gcatttacat ctatttcttc accatttatt ttagctgtat agctattaaa attaaccgat
      361 agatccttat attcaaactt ctctaaatgt ccataattct tcttaattaa agaatctatt
      421 ctagctttta agaccggcaa tgaaaatggc ttttctacat atccgtctac taaattagta
      481 aatgcatcaa ttttatattc ttcatcacta aatgcagtca agattagaat tggtaaattg
      541 cttttctttc taatttcttt caacacttct aaaccattta taaaaggtat ctgaatgtct
      601 aagataacca aatttatatc gctattaaat tttgacaagg cttctcttcc atctttagct
      661 tgaataacag tatatccaaa ttctgaaaga tattcactta ttccctctct tatcatatta
      721 tcatcttcaa cagttaatat tttcatatat acctccactt aagcctatac actagatatt
      781 atacagcata ttatggctct ataatatcta gtgttggtga cgccttctgt tgccaatact
      841 ttttcttttt tttaataaat gcaaacagac accttgaaat cctttagaaa aaaggcttct
      901 ataatatttt gtgttgttgg aaaatcgaag gcgattttct actaacactt tttcttcgtt
      961 tcaactcaaa aagagactca cttttggtaa aatttagtcg accaaaacat caccctacca
     1021 aaggagtcgc tctatggata atcatactag aaaattactc ggattaacag ataaacattt
     1081 attttttgat gaagaatggc tgattgaaaa agaatataaa ggcgttcaag cgcagtttat
     1141 taaagggaga ttggatgact caccttccca ttgtgaacac tgtggatcga ttcgtattat
     1201 ccgcaacggt tcctatacga cacgtactca gatgttgaaa gtcaaagaaa agttgactat
     1261 tttagaatta aaacgtaccc gttttctatg ctatgattgt gggcagacct tttctgcgaa
     1321 aacggactta gttgatgaac atcatcaact aacaaaggaa ctgaagcagg cgattctcat
     1381 agaactgtac gaaaatcaat cacgtaatct gaaaatcaga agaagtaccg tcggctcaag
     1441 cgatattgga aactcttact caaagattcc acgacattgg agccgctcaa acggcattat
     1501 catcgactgt ttaaacgacc aattagtcaa acagaaatcg tcgatgaacc gttgtgcaca
     1561 atggtgtcct gaacgtacag cgacaccttc catatccaga gcagtcgcca catcacgggg
     1621 atggatgcca tccaaattaa aagcaaccac gccagtatga tgttcaggat cttgcggtcc
     1681 gtaaatggtg ataccgccga tcgcttgtaa tttaggtaaa acgtaggcaa ccagttcttg
     1741 ctcataacga tgaatattct ccataccaat ctcattcaag taatcgattg ctcctgcaat
     1801 gttcggagta ccagcttcaa atttccatgg cagctctttc catgaactat cgtaaagatt
     1861 gacaaaatct atcatttcac cgccgaattc gactggctcc atttgttcta acaattgacg
     1921 tttgccatat aatacaccga ttcctgtcgg cccgcacatt ttatgcccgc taaaggcgaa
     1981 aaaatctgca tccaacgctt gtacatcaac tgtcatatgc ggtgctgctt gggcaccgac
     2041 tggactatcc tcaactacaa cagcctgttc aaccctcagc cgtttcaagc acaacaaata
     2101 accttctgga tctggtttgc cgacctgaag atcatcgatc gtcacaaagg aatcaatttt
     2161 ttccttaatc atcggatgtg cttcaaaaaa atagtctagc tcccgcagtt cagaattgag
     2221 actactccga ttttccagtg ctgctgatcc gctgctcaaa atgttcagca tccactggtc
     2281 tcccaaccgc ttgacttatc ccttcataaa aggacagttc tgctgccgtc ccaacacctt
     2341 gttgatagtc tgcaaaagga attttatagc cgaactgctc cagtaaatag gcttcagtaa
     2401 atagtgatga atactctcgt aaaacatcca ttcactgtca atcatcgtcc catcaaaatc
     2461 aaaaataagt gctttggtca tcttctttcc cccttaccca aaacaagcaa aagctgtgtc
     2521 ctgctctctc tacagtatca gcataaaaac tgccgtctga aaagtcaaag gtgaaagaaa
     2581 tgtggcgcta taatttctag gactgatagt aaatcattct atcagtcctt ttgagcataa
     2641 aaaaacaaaa catacagaag ctccgttaga attgaatcac cacaacacaa tcaaaggagc
     2701 gttctgtatg cctgatcttc atcttatcaa actattacta ggaatcaaag atggaaacat
     2761 tcaaatcact catgtcgaga agaaaaaaat acattcaatc aacagtatcg ttgtttctgc
     2821 cacgctcatt aaaacgattc agccctgtcc tcagtgtaaa caggtgaatc aacatgggct
     2881 gatcgttaag aacggaaaga aaaaatcgct ggtccttctc aataagtgtg cgaatcaacg
     2941 gacttatctt gctttggcaa agcaacggta cctctgtcgt ggctgtcaca cctattttac
     3001 ggcgaatacc tacattgttg aaaaaaactg ttttatcgcc aaacaagtac attataaaat
     3061 attggaggaa ttgactgaaa aacaagcaat gacgacgatt tctaaacatt gcggcgtttc
     3121 ttggtcaacc gtttcgcgaa cactcgcttc tctgctcccc atgacaaacg tgaaaataaa
     3181 ttggttgcct cgctgttcat tggtagatga atttcgttca ttgaaaaatc aagtcggtcc
     3241 ctattctttt tcttgtatgg atggcgatac tgggaaatta ctggatattc ttccttcacg
     3301 taagaagaaa gatctcattt cttattttat gcaatttgaa cgacgtgcac gtttaaacgt
     3361 aaaaatactc gtaacagata tgaacgcctc ttatgctttg ctgataaaag agtgctttcc
     3421 tcatgccaaa ctcgttgtgg atcgctttca catcgtcaag cacctcattc gcagttttaa
     3481 ggatatttga ctatgagtca tgaaaagctt cgaccgaaac gatccaatac aagccaaaca
     3541 ctatcgacaa gtgaaggctt tatctcgact tctgatcaaa cgacaggaca cgcttgttta
     3601 tgataaatgg acaaaatggc gtaattttgg ttgggcctat ttaaccgaaa gtgaagtcgt
     3661 agaacgtcta ctttctactt ctgatgaact acgaatagct tatgcttact atcaagaaat
     3721 tcttcaagct ttttatgaca aagaagcgga catcttcttt cagcttgtaa aaacaatgcc
     3781 caagtcggtt ccaagagaac tgcatcatat aaaaaaagcg tttataaact atgaatccgg
     3841 cattcgttta gccttagagt tgccttactc aaatggcaaa atagaaaatc tgcataccca
     3901 catcaaagca ttgaaacgaa tagcttacgg gttaggatga gaaccagaat tttcttaatc
     3961 aatcatctga tcacttatta aacataaaaa aacatctaaa gcagaaaatc aattctacct
     4021 tagatgttct tatatcactc tatcagtcct atttgacaaa gagccagaaa tgtttgccga
     4081 ctatacacta gctttgttgt gcaaaatgct gagtatcaat ttccacaggg attgctagaa
     4141 tatctttcag ctgaatgtat aaggcatcgc taattttctc ttcccgatcg ttcaaccgat
     4201 ccactaaaat ttctttgccc tccagcgttt gaaaaacata ccgttgaatg ttgcctaaaa
     4261 taaatgattc cttccataga accggaatct taaccccctg aggaaccggt tcaaattgaa
     4321 tcagctccgg tcttaaataa taaatgcagc gattgccctt agcggaaaac tcgtgggtat
     4381 actgattcag ctcataccat tcgaaacgat tatagttgcc gataaattcc gcaacaaatc
     4441 ggctctgagg attctgatac aattccttcg aggtggaaac ctgagccacc cgcccagctt
     4501 ccatcacaaa aatccgatca ctgattcgca tcgcctcttc ttggtcatga gtcacaaaaa
     4561 tcatcgtcat ccctagcgat tgctggatag cgcgcaaatc ccgctgcagc tgttttctaa
     4621 ttcgggcatc caaagcactt aatggctcat ccagcagcag gactttcggt tcagtgacca
     4681 atgagcgagc cagtgccacc cgttgtcgtt gaccgccaga aagaaactgg acatttttat
     4741 ctgcctgctc cgtcatatcc accaatgata gcatctcaaa aaccttttcc tgaatcacct
     4801 caggactcag cttctttacc ttcaaaccaa aggctacatt ttcaaaaaca gtcatagttg
     4861 gaaacaatgc atactgttga aaaacaaagc cgatattgcg atccttcgta ggcttatcgg
     4921 caatattctc attgtccagc acaatctcac cgctctttgg ttgttccaat cccgtgatac
     4981 agcgcagcaa cgtagacttt ccgcacccgc tgggacctag taaagtaacc agctcaccct
     5041 tttcaatctg aatttcatca atagctaaaa tctgtttttc atcaaaaaac agattgagat
     5101 catgtatttc taaaaacatt ttccgtcaca actcctttaa gggcttcttt ttcgactgct
     5161 tctcttccct cgtctgctta agggacagct gttgcttttg tcgataagtc agcataaagg
     5221 tagttaaagc aattcccacc ataaacagca aatacaaaac catcacagcg ctagccaaat
     5281 ggccgttttc gttcatccgc cgcagcatat agatgcgtac cgtctgaaat cgaccgccaa
     5341 tcaacaggtt cgtcagcatg tactccccaa aaaccgaaga aaaaatcatc agagaaacta
     5401 gggtcacacc gatacggatg tttggaaaca gcacctggat aataatcgta gacatcgggg
     5461 cgcccaaggt ttctgctgca tccactaatt ccttcagatt aattaagcgc atctggttgt
     5521 taagactaag atagacaatc ggcaacgccg taataaacaa tgcacctacc aacactacaa
     5581 acattgggat accagtttca gcatacgttc gaagcaaagc cgttaccaaa atcactcctg
     5641 gtaaagcata cggcagcagg agcagctttt ccagccaacg attcaaacga gggaaataca
     5701 aatgaatcca aataatggtg ggaatcatca acaacagaat cgtcaccaag accacacctg
     5761 ctaataaaag tgagcgaccc accgcagcaa taaattccct gtccgtcagc agctgttgga
     5821 accattgcaa ggtaaaatca ctaggtaaaa gcgattttga ccatttagtg gagctggcat
     5881 acaaaatcgt tccaataatc ggcagcaagt aatacaggac aaagcagaac aaaatcggtt
     5941 ttattaactt tttcacgtct gcaccacctt gcctttcttt tgaataatca acattgccgt
     6001 taccagcagg acacctaaag cgactgccaa cgcactgcct aaattaggct tggcgaacac
     6061 atctcccgac actaaagccg caattcgaat cgttaaaaga tttacattac tgctggtcag
     6121 ggcatacgct gtttcatagg tccccatgcc attagcaaat aagatgacaa aggtgctggc
     6181 aatcgatggt cgcaaaaaag gcaaagccac cttgaaccaa gcatacgctg atgaagcgcc
     6241 cagcaattgt gcggactcca gccattcctt tttcacttcc ttcatgctgg gatacaaaaa
     6301 caagacccct aacggaattt ggaaataggt atacgtgatg gtcaatcccc accaagaata
     6361 caaattgaag tccgctagaa atggcaaaat cattttcagc acacctacat tccccaacaa
     6421 aataataaaa gagaatgcca gcggaatccc agcaaaatta gctgccaaat tggaaacaaa
     6481 ggtgattttc tcctgcaatt tagtcgatag attcattaag cataaacata aaataaacgt
     6541 cccgattaac ccaatcacac tggagctgac agcaatcagt acgctgttaa caaaggcctg
     6601 tgtgtaatat ttgttgctga aaatatcact gtaattggcc aatgtccact ggttagttaa
     6661 atcactgtgc aagctgcaca gaaccataaa aagcagcggc agcagtaaaa atgcacagaa
     6721 gataaaggag gcaggaacca gaaccaagcg ttttttcatc tctttcatcc cctcatcaat
     6781 tcttgaatgt cctgcatttt ctctgacggt gcaattccta ataaccagca aaacaatgga
     6841 gccgccagca gctgtggtac agtttgttcc ttcagctttt ggcgtttatt tgaaatcaaa
     6901 taaaaaggaa cttcacgatg tgcagccaag ctgccgccat gcagtccgtg cgcatccatc
     6961 ccgtgatccg cagtcaccac gacttgataa cctttttgca gccattcagg aaggtaaata
     7021 gccaataatc tgtccgcctg attaaccgct gctgcatatt catgagaatc ggcggtaaat
     7081 ttgtgaccga catcatcaat attcatcgaa tgtacgacca aaaagtctgg ctgatactga
     7141 ttcactaaat aatgggcatc cgcaaataaa tgcgaatcag ggtaagtgtc ctcactataa
     7201 aagatgccat gctgaatcag tcgctcctcc tgatgctgga tgcgatcaac caaacgatta
     7261 tagggggctt gattgtacaa ctcgctgtac cagtggtaag cggcggctcc tgtttttccg
     7321 ccagcttcct taactaattc aaacagcgac cactctttgg atcgctgagc atggtaatta
     7381 ctatagatgc cattttcata ggctggaacg ccggtcatca gtacttcgta taacggacgt
     7441 gaattgctgg gcagctcact ttttaccttc aataagcttg cttgctggtg ctcaactaaa
     7501 tgattcatga acccaagctg ggcgacggct gtgtcgtagc gacagccgtc tagtataact
     7561 acaatcagtt tgtcaagtct actttgcata ggtcagtacc tccgcctgcc aaagctctgg
     7621 aagctctaca gaggtcttgt tccaagcttc attgtcttca actggcaccg ccgcttcata
     7681 agcatcctcc ggcagtaatt tttcagctac ttctttaggc agctctacct tgcgaatagg
     7741 tcgcgcatag ccctttgcca aattgatttg cccttcatca gataaaatat attcccgtgc
     7801 tagctttgcc gcattgggat gaggggcatt tttattgatt aaggttgtat acccgctaat
     7861 gatggagccg tctgttggca cagttacctc aaaacgctcg gagtcaattt ggtcacgata
     7921 atttagtcca ttgaagtccc aaacaactgc cacatcgatt tcaccttttt caagatttgc
     7981 tacgctggca tcaacggtgg acaaacggcc tttttctgcc atttttttgt agtaattcag
     8041 acctggctga atatcctttt cgtcacctcc gttggcaatc gccgcagcca atacagcaaa
     8101 ttgtgcttgg ttggcaatcg tcacatcccc cacgctaact tgatagtcac cctcttgcag
     8161 ctccttccag ctagtcggtg cttgcttcac attctcttta tcggtgataa aggaaatggt
     8221 tcccgtatag cttaacaacc aatccccatc atcatccttt gcccaatccg gaatttcctc
     8281 ccaatagttg gtcttataag gtaaggttaa ctctttgctt tcagccaacg gaccgaagct
     8341 gataccgaca tcaccaatat cggcggttcc attttttcct tctgattcaa acttagccaa
     8401 ttcttctgca ctggacatat ccgtatccac gtgacttaag ccgtattctt tttcaaggtc
     8461 gttccaagtg cctacccaat ttgcccaagt atccggcatt cccaagctgg caatctctcc
     8521 ttcttccttg gctttttcta taatcgtatc taaagagtcg tctgccgcag ccgattgctt
     8581 cgttgcttca cctgagccgc agcctgtcag caggatactt actgttgctg cacaaaataa
     8641 accgatagtc atgttacgaa ttttttcatt cattttcatt cattcttctc ccctttcgat
     8701 aattccattc taacaaagct ttgtaatgag agaatcagtt tcttgaaaag gtttcatgaa
     8761 caatgtaaag gttgtatatg gtttgtaaat ttcgacgaat tcccgttaac cttctaaagc
     8821 ttttgcggta tgatagtaga aaaaaggagg ctccctatgc agtattatga tcagcatctc
     8881 cacacgtatt tttcctttga ttcagaggaa aaatttgaaa actatttagc ctatcagccg
     8941 gagtttttcg tttctacgga tcactttgat ttaaacaatc catctaccgg aaaagatgac
     9001 attccggatt atccagccta tataaacaaa ctggactctt taaaagaaac ctattccacc
     9061 aaatttctgc gaggtatcga gattggcgtt gttccaggac aagaagagcg gatcgtggat
     9121 tacctcgctc agcatcctta tgatttaaag ctggtaagta ttcatcaaaa tggccgcttt
     9181 gattatatgg acgatgtggt tttaaccaaa gatcccattc aaactaccaa ggaatatttt
     9241 gatcagatgt tgcaggtctt gacggatttt ccagaaggag atattttgac tcactttgat
     9301 tatggattac gccgcttcac cttcacagcg gaagaattcg cggagcattt tgaggctcag
     9361 ctgctaacga ttttcaaaaa ggttgttgaa cgagaattgg cgatggagtt aaatgccaag
     9421 agcttcttga aatacggcaa cgaagacctt taccgttatg ccgttccttt gtatcaaagt
     9481 gtcggcggaa agctctttac cttaggctcg gatgctcatg ttgccgaaga ttatcagctg
     9541 ggctttaaaa aaatgagtca gctgttggca gactgtcagg tagaaaagtt gctagtcatt
     9601 caaggaaaag aacgccgcct gacgccgctg ccgattttgt gattcctata taatagaaga
     9661 aagtggtgaa aaaaatgagc aacctgtggg actacaatca agaggcaccc atccattatt
     9721 taattgcgcg acattgggat gcgttgaaaa ttgaggcggt ttgccgatcc ttgttagcag
     9781 ccgttccaaa acaacaactg gaaaattttt tggtggctga cagcttgcag cgggaaaaag
     9841 ttcaggctta ttttgcagcc tttaaagatc agcctttgga atatttgcat gcgcagtttc
     9901 atctttttta tcaagtagcc gccccagatg actacaacga tttacgagga cagctgcaat
     9961 tgacctttca agcggatgaa accgcctata ccgttttatt gggaatggct cgccttggtg
    10021 atcaggcgaa ggtcgagtgg cggattttcg atatttgatg gcaggatcaa ggcgtttcag
    10081 ttgcaaacct tcctctcttt caattagaaa aaccgcagca ggaagaagtt tgtgtgctgg
    10141 tcaccgatgc tggtgaaatt cgtctccgct tatttcctga ccaagctccc ctggctgtag
    10201 ccaactggcg ctccctagct gagcaaggcg tttatgacaa cacacctttc ggctcgtgtc
    10261 attaaggaat ttgttattca aggtggcgct ttggatggtt ctggtgatga agctgtttcc
    10321 agctatgagg gctttttcgc tgatgaagtg catcgcggac tttatcattt taatggcgcc
    10381 ttggccttag gggaccacgg cccccacacc aacggcaacc agttttttat tgtgcaaaac
    10441 acaaaggcgc aggctgacct gctaatgtaa agcaagcata cttggagctt ggaggactac
    10501 cggagctgga tggtcgttat actgtttttg gacaggtata ccaagggtta gaaatcgtag
    10561 cagcgatcgc agctcagccg acaaacagtg aagatgtacc gattgagcca gtgttcattc
    10621 gacaaattaa cttcgaaaaa acatcgtaaa aagacactca ctgacttcag caaaagctct
    10681 attcttgagc tggctgcagc tagtgagtgt ctttcattta tccataagat aaagcttgga
    10741 ttcaataaat aaataaatgt tttggttaaa gctgtgacca gcaaatgagg aaccgtgcga
    10801 gccagttgat ctgccaatgc aaacatttct ttaggtgaag gcgctggcgt gtctgccaac
    10861 ggatactcca gtcccaatgc ttggtaaccg tgcataccat agcagtggta cggtagtaat
    10921 tctaacggta cgaaccgatc aagatctgcc acaaaacaag caatcgtcag cagctcggtc
    10981 aggttcatat tgatacctgg aaccacagga actcgaattc gcacttcgca gttaggatac
    11041 tcaattaaaa agcgacgaat attcttcaaa atttgttggt tatctaagcc ggtccattgt
    11101 ttgtggagct tgctggtaaa catttttaca tccacaaaca atgtatccac atagggagct
    11161 accttttgga tttcattata agcaccacaa aagctggttt ccagtacggt attgattcca
    11221 atgtatttcg atttttgcaa aatttcttta gcaaaatccg cctgaaccag tgcctcgcct
    11281 ccgctaatgg tcactccgcc atccgagtga aagtaaaaaa cagcatcctt ctcaatctca
    11341 tccattacct cttcagcggt catggtctgg ccaaatcctt tttcaatctg cttgctttgt
    11401 gattcgggaa tcgaacacca ggcacagcgt aatgggcagc cctttaaata aacaacggtc
    11461 cgtaaaccag caccatcatg tagagaaccc tgttctattc ttaatactgt tccttccata
    11521 tagatttttg aattacacac tagtttaaac taaaacacac aaggaaaaac ttaccattta
    11581 atcataaaat tagctcatat aaatatacta ggatgtaaaa tagactggtc aaaatctggt
    11641 caaaaaagag gactaataca ggtagtcctc ttttttgtta ttttaatatt ctaaatcatt
    11701 ttttattggt ttattccctt attcgatgta gactcaaatc actatatcac tagatatcaa
    11761 acggcgtcat acggaatcaa aaacatcaaa tttgacctgt taaatggtca ttgaataaca
    11821 ttcaaaatca ataaaacgga gtcagacacg gagtcaaaaa cgctgagagt aaaaaatata
    11881 tacagccttg taggtaaagt accacagggc tgtatatttt ttatttcaga ttaccttaaa
    11941 tgattccatt tactttttat ctcatttttt cctactagtt agtatcaaat actctcctat
    12001 tttctcaata aactattttt ggatacatgg tatctgaaac agctaaaacc ttgtcattac
    12061 agcaaaaaat cacgttatcc agactgtaga caaagtccat tttatggatg atgtctacag
    12121 tcttttcttt tataatagat ttatgaaaac gaatgcaagg agtgatagct atgatgtcta
    12181 aaaacaatac gaatggtaga aatcaatttg cgatgcttac gattga
//
LOCUS       DACDYJ010000074        12129 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDYJ010000074 DACDYJ010000000
VERSION     DACDYJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.074"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            97..336
                     /locus_tag="IWM51_002229"
     CDS             97..336
                     /locus_tag="IWM51_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971625.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            463..1152
                     /locus_tag="IWM51_002230"
     CDS             463..1152
                     /locus_tag="IWM51_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAP9971626.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            1158..2324
                     /locus_tag="IWM51_002231"
     CDS             1158..2324
                     /locus_tag="IWM51_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP9971627.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            2406..3077
                     /locus_tag="IWM51_002232"
     CDS             2406..3077
                     /locus_tag="IWM51_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9971628.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIKMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAKMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            3130..5106
                     /locus_tag="IWM51_002233"
     CDS             3130..5106
                     /locus_tag="IWM51_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9971629.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            5119..5871
                     /locus_tag="IWM51_002234"
     CDS             5119..5871
                     /locus_tag="IWM51_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9971630.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            5887..6648
                     /locus_tag="IWM51_002235"
     CDS             5887..6648
                     /locus_tag="IWM51_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971631.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            6661..6921
                     /locus_tag="IWM51_002236"
     CDS             6661..6921
                     /locus_tag="IWM51_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971632.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            6918..9008
                     /locus_tag="IWM51_002237"
     CDS             6918..9008
                     /locus_tag="IWM51_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9971633.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(9044..11101)
                     /locus_tag="IWM51_002238"
     CDS             complement(9044..11101)
                     /locus_tag="IWM51_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP9971634.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(11246..11437)
                     /locus_tag="IWM51_002239"
     CDS             complement(11246..11437)
                     /locus_tag="IWM51_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971635.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            11625..12080
                     /locus_tag="IWM51_002240"
     CDS             11625..12080
                     /locus_tag="IWM51_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971636.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
BASE COUNT         4150 a         1931 c         2413 g         3635 t
ORIGIN      
        1 aaacgaaaga aaaagataac ccaagactga agtgtagagc gtatgtgatc ataggctttt
       61 ttatttttgc aaaaaacaag aaaggtagga aaactcatga attatgaaga aatcgaaaac
      121 agaaaaaaag tgtcgaaaga aatggaagaa aagttgctaa aaatgatgaa acaaaaacac
      181 cttaaaagat tatctgtcat gcaatatatc aatgatatga agataacagg aaaagagaaa
      241 gcttgtttgt taggttcaat gaaaaacttt gaacagttga ggcgaactta tgtaaaaaca
      301 ggttccaatt gccaactatt attagaagtt tcttgacgat tatgatatga aaatgatcaa
      361 ttatgatgtg gcattgttat tgaataaaaa gaatagtaaa atttaacatg gtttatcttg
      421 gttagatgta ggtaaattaa ttcatatata gaaggtagat agatgaataa aaacaataaa
      481 actttattta atccttacgt gggcgtaatc attgtttttc ttctttttag ttattttgct
      541 tatcaaggtt atcaaaagga caaaatcgct gaaaaagtag atggtgtaac aaaaacaatc
      601 catattcagg aacaggctaa aacctatcaa gataatttaa ccagtttaac ggatatttct
      661 aagagacaac tgattactaa tcaaaaacag gcattagaaa aggatctcaa aggtgaaatt
      721 gctggaaaac ttgttttttc ttctacagga tttattctcc ctgtttttca aggagcgaat
      781 agcgacacgc ttagtttagg agtagcttct acttattatc tagatactga aatgggaaat
      841 ggaaattata ttcttgctgg ttataacgta gatacaccaa gcgttttatt atccgatatg
      901 actgatttat ctgaaggaga agaaattgat ctaatcgatg ctcattacaa gtatcagtat
      961 gaagtctcca aaagaggaaa aatttctgat tacagccagg tagttcaatc taaggaaaac
     1021 aattcctttt tatccttgcc aaaagcaggt aaaaaaccac tactcacact gttagccttt
     1081 caagcaaata atatgaaaaa gtgcgatgtg atccagtgtg aattagtcaa tgtagaaaaa
     1141 atcaataaat aagggaagtg agtcacattc aagaacttgt agaaaaattg atcaataaaa
     1201 tgagtgccat ttttctgtta ctgttggtat ccattctata tttaatatgg acggtggggt
     1261 taaatcctcc tacgatatta tttatttatg ataatgctgc acagttagtg acgtttttcg
     1321 taggctgttt gtttcttttg aatctgtaca aggtaaagag aaacgatcta ttcttcattg
     1381 cagcaggcat tcttttttac cttttctttt ctttttttag aagctaccga ggagatactt
     1441 tctatggtga tctgatcgtt ccgacaatta ttgctattat tttgattgta aaatggacga
     1501 aatttactgt aattgatcgt agcttgtatc ttgtagtatt ctatgtcttt ttactcgtaa
     1561 cggtttatcg agtatttact gagataccag taccagaagg acaaagtatt tggaatccat
     1621 cgaacaaatt atcagatatt tggatcaata caaatacgat aggtagttct ttaatgacat
     1681 tagcccttct tatcacagga tttgctagtt catttggaaa atggtacatg cgattgctat
     1741 cattaccagc gttgattctt gctggggcaa cgatttgggt ttgccaatcg aaagcagcat
     1801 tatatgcgct tgttatcttc attatcttag aaattttacc taagaaggtt tttcgaaata
     1861 cacacttgtc cttttggatg tatgcagcag tttcggttct agctttaccg atttcttatt
     1921 ttgctgcaat gtcagacaaa gttcacttat ttacaggacg agaagaaatt tggcgcaaat
     1981 tctatgaaac attaggcgaa aaaacaggac aaatatttgt agggatgaaa cctttctttt
     2041 tccatagagg aaatcagatt ctaggtaatc ataatacgta tcacgcactc gtcaatcact
     2101 atggattgat tggttttgtt ctatcgatta ttcttttgct tagtttctta tggttgctcg
     2161 taagaaaacg tgagctgaca aatggacaac taacattctt gtgggctttt ttagcgatca
     2221 tgtgtcaatc gttcatggaa gaaacattaa cgacaatcag ttgggtgcca attgtattct
     2281 taatgttagc aatggctaca caccaatata aaaccaccag ataaatgaaa tagaatagta
     2341 ataagagaaa acactttttc gttgtgaaaa agtgtttttt gttttgtctg aaaggaggca
     2401 gatggatgaa aaaatggtta tttggttttt taggagtcgc tttaatcgtc gtttgcagtg
     2461 tgtttggcta tgttagctac caaaaacatg aaggagaggt gtttaagcaa aatatagaaa
     2521 aaaagatgcc cgtagatcaa atcaatgcac acgcaaagag ttataaggag gatgcaacga
     2581 acgtcaataa tgatatgtct ttggggcaaa tgttgtccat acaaaaagaa gcaatcaaaa
     2641 tgggcgtcaa taaacaagta tttgcacaaa ttcagattcc tgcacttggt ttagcattgc
     2701 caatttttaa aggggcaaat caatacactt tgagtcttgg agcggctact tatttttatg
     2761 aagatgccaa aatgggcaaa ggaaactatg tcctagcagg tcataacatg gaaatgccag
     2821 gtgttttatt ttctgatatt caaaagttgt cattagggga ggtgatggac ttagttagta
     2881 acgatggcgt ctatcgttac aaggtaacgc gtaaatttat cgtacctgag tactttaaat
     2941 taatcgatgg cgttcctgaa gagaactctt tcttgtcttt accaaagaaa ggagagaaac
     3001 ccttattaac gttgtttact tgtgtttata cgtcacaagg aaaagaaaga tacgtggtac
     3061 agggagagct acaataacaa gaatttaatc aacgcgagtc gttgaaatat gaaatgagga
     3121 gagtaaaaaa tggcaataaa tcggtatgta aaaaaagctg gaatgattgt tggaatgtta
     3181 ggaatcattg caggctgctt tcaggggtat cgagcttatg cagaagatgt cacgcaaaaa
     3241 acgccaccag aaagggtcaa tatcacggtt cataaattaa tgtacgatca aagtacgcaa
     3301 ttgaacgttg atattgacgg aatcaaaaat gatggttata cgcacgatca atatcctaca
     3361 ggtgtaacga aatacaacaa ggcagattat ggtgatgttg agtttacgtt aggaaatatt
     3421 acggatcaag tattaccgac tgaagattct gatctaacca acgcgaaagt ggacgaaatc
     3481 gttaaagatg tagaagacaa gggatctaac agtgagtacg ttaagaatgc aaccaataaa
     3541 attacttcag ctgtagatga aaatggtgag atcacttttg ctgatcaacc agcctatgtc
     3601 aattccaaag ggaatgtata tgtggtttat gaaagtaaat ccgctgctgg attagtcaca
     3661 caaaaagcaa aaccaatggt tgtgattgcg ccaatgacag acaatacagg atcaggattt
     3721 ttaaaagata ttcatttgta tccaaagaac attgtttcta agttgagttt tgaattaacg
     3781 aaatttggtg acgatggcac agctcaaagc aaacaaacac ctttaaaagg ggcaaaattt
     3841 gagttataca agggcgaacc tggaaaagga acaaaattag gagagcttgt atcggatgat
     3901 caaggaaagc tgactgcaac tgatttgacg ttaggaaaat actactttgt ggaagttcct
     3961 tcagaagttg tagtgggatc agatcaagaa ccaacagcag accaatattt gttaggcgca
     4021 gacgctcgca acgatgcgca caataagtta acctttgaaa ttacgaatga cggcgtgaca
     4081 agtgatttaa aagcttccta cgtgaactat aaagcaccag tgatcgataa aacagtgaca
     4141 aatggaacag gagaagaaca ttctttccaa attggtgacg cagtgaatta tcaaggtaca
     4201 attcatatcc ctacagatat cgcaggtgga acagatggga ttacagtaaa tggtgtaaaa
     4261 agtgaaactt ctccttacag tgtatttaaa tggggcgaca cagcaggtaa aggactttct
     4321 tatgttgctg aaaaagcaga tattaaagta acaaataatg atggttcagt cgtattaaaa
     4381 gaaaatactg actataaaat cgaaaattct gatagtggtt ttgtgatcga ttttatcgta
     4441 aataatggac aagtaagtga tacagttgcg aatttacatg gtcaagatct aaaaatgact
     4501 tataacatgt ttgtgaatga tagtgctgaa atcgcaaatc cattaacaaa tagcgtggat
     4561 ttcgtttata acaataatcc atttaatcag gaagaacacc acgaaaaaac gaaagctgac
     4621 gttgtgactt atggcgcaaa attcttaaaa gtcgatagcg gcctatttgg aacaggtatc
     4681 aaagcaacac cgcttgaagg cgcagaattt gcagctaaga atgcagaagg taagtactac
     4741 ggtggactta ctgatacaga taaagatggt gtcaaagaag cagtttgggt agacgatgta
     4801 gcaaatgcag caatcctaaa atcagataaa gagggtcatt ttgaaatcac tggtttaact
     4861 gaaggagatt actcactgga agaaacaaaa gctcctgaaa actatcaaaa attaacaaaa
     4921 gagatctctt tcaaagtaga caaagattca tacaaagaag aaaaccgaat cacgatcaaa
     4981 aataatcaaa aagcttccgt accaatgact ggtagcaacg gtttccaatc ttacgtattg
     5041 atctcttgct tgttgcttgg ggcaggcgca atttctgcag ttgtttactt taagaagaaa
     5101 gcctagaagg atcagctaat gattatcaga caccctaaga aaaagcgaat catgggtaaa
     5161 tggatcattg ctttctggtt actttcagct gttggtgtct tacttttgat gcctgctgaa
     5221 gcgagcgtgg cgaaatacca acaaaatcag cagattgccg caatcgatcg aacaggaacg
     5281 gcagcagaaa cggattcttc tttggatgtt gctaagattg agttaggcga tcctgttggt
     5341 attcttacaa ttccaagtat ttctttaaaa ctaccaattt atgacggaac ttccgacaaa
     5401 attttagaga atggtgtagg gattacggaa ggaactggcg atattacagg aggaaacggt
     5461 aaaaatcctt taatcgcagg gcacagtgga ctttataaag ataatctttt cgatgatttg
     5521 ccttcagtta aaaagggaga aaagttttat atcaaagttg atggtgagca gcacgcttat
     5581 caaatcgatc ggattgagga agtgcaaaaa gacgaactac aaagaaactt tgtcacttac
     5641 ttagaaccaa atcccaatga agatcgagta acgctaatga catgtacgcc taaggggatc
     5701 aacacacacc gtttcctagt gtatgggaag cgagtaacct ttacaaaatc agaattaaaa
     5761 gatgaagaaa ataagaaaca aaaattatct tggaaatggc ttttaggaag tactgttttt
     5821 ctttcagtca tgatcattgg ttcgctattt gtttataaaa agaaaaaata aaggagggta
     5881 accaacatga gaaatagaaa atggctattt gtttgtgcag ccgctctttt ttctttcact
     5941 gcctacacac agttgacgtt tgcagacaga caagaaagca cggatagcac agaacaaaca
     6001 atccaaacgt cagatagcag cacgattcaa agcagcgatg aaaccgtcga ttcgtcaaca
     6061 gttgattctt cagaagaacc taaaattgct gaagacgatc aaatagtcaa acagaccatt
     6121 cacgttcaaa aaattatttc ttccgaacaa ttagacaacc aggggcaaaa gcttgaaaaa
     6181 caagaaggag taaatggcgc gaagttttct gtttatgatg tatcggacat tttacaaaag
     6241 atggatgtta aggatctcac tactgaccaa attgaatccc aattaaaaga tcgagtgaaa
     6301 aaactatcca gtgatcaact gaagctaatt tctaatggag aaacaaagac aattgatcaa
     6361 caaacaggtg tctttgaatt ctcggtagaa gtacaggcaa atcagaaaca agcttattat
     6421 attgtcaatg aatcaagtcc tgaaaacatt tctaatagcg aggatatttt gctattaaca
     6481 cctgtttctg ataaaaatgg tgaattctta aaggatgtgt ggatttatcc taaatcagaa
     6541 gcttcgcaac ctaaagaaga agtaaaaaaa atcgtatcta ctggcgtaaa gaaaaacttc
     6601 tttgagaatt gttgggattt tgttactcac ttgttttcta gagattaagg aagaaataaa
     6661 atgaaactga tcaaattatt attattcaat ttttcttgtt taaccgcagt ctttttgtac
     6721 tgcggtttat tattttctaa actatctatt ttgccaagct accaactata cctaatgagg
     6781 attttaaaca taccagctgc ttccatgccg ctagatatta tggaaaagct cattaggaat
     6841 ccattattta ctacgatcaa tttgatcgtt attgtcggaa tttcactagt catttataaa
     6901 aaagagggga tgaagggatg aattttaaac actcgtggac aagacggtta aaatatgttt
     6961 ttcccataat gatgatgtta ggtacattgt ttgggttaaa aacagccaat gtctttgctg
     7021 aaaaagttat tgtcgatccg tcaaatccaa tccagaacgt aaagaataat tttattattc
     7081 caaaagatgt tcctaatgca aaaacaaata ttactgtaaa cggagcgtca aaagtttaca
     7141 aatatcatgt tgatgccaac aaaggtggtt ttacagacga ctacgtagga ttagtggagg
     7201 gaaacaaaaa tatacgctat ccttctttcc aaaaagaata cggtgaaaca aaccttgttt
     7261 tagaaggcgt aacaaccaac accaaagtca atattgacta tggaaaaatc ggtacgtata
     7321 acgggaaaaa agtcaatatc aagctagttc tatctaatat tcacttatac tcggatacgt
     7381 tgccgtggaa tctactagat aacaactata caaaaactca ttttcgagac gatggctata
     7441 aaaatacgaa tggtgcaatg agtaaatcta aaaaaagaac agtactttgg atctcagaca
     7501 atcttttctc aggaattgtt taccattcaa cacaaatgaa tgtgcagcta gtagccacat
     7561 atgaggacgg tagccccgtt caattttcag gagatacatt tatcagtttt aactctttga
     7621 accctgcagg aggaaaaagc acggatctta aaggagaata tgcccactat gacaaaatga
     7681 atacgacaga ttggtatgtc agaaaagata cggttctttc agaatttaaa tctttctaca
     7741 acaatctgaa cgtggtaggt ggccacccag gaggaagctc taagctgaca caagcggata
     7801 atgatttcaa taatttgcat gataaattag gagatccgaa gtttggtcaa ggtactgtgt
     7861 cctttaaaat atctgaagca aatccgacgt ttgtgattgg ttcttctaat gttcaaacct
     7921 ggtttacttt atcttctgcg acaatcttta gtgttgttcc tgatcaacct gaaaaaacag
     7981 gggttgataa aaatggaaat aacgtgaacg ataaaatgtt gcaagtagga gacacgattc
     8041 aatatcgaat caaacagaag gtgaatcgat taggagtgga tttactagcg gtttacgatc
     8101 gatttgagtt aatcgataat ttaccaaaag aagtaaatta cgtcgatgca cacgttgaaa
     8161 gtggtacaaa taaaaaattc gatgtatctg gtgaagtgac ctatgacaaa acaaaacacc
     8221 aggtaaaata tgcagcgaaa gctgatacat taaaaaatcg aatgaagtat aacggcgaaa
     8281 cttacgaatt agtgatcaat gtaaaagtca atgaattggc caatcaaaat agtgtagcca
     8341 aaaatcaggg aacgtcgatc attaataaag tagaaaagga tacaaatatc attaccgttt
     8401 atttccctaa aattccagtg aaagaagtac aacaaaatgg aaaagatgta aacggacgaa
     8461 atgacggtaa gaagggtacg ccgacagctc ctttaaacgc agggtctgaa gtacaatatc
     8521 tagtcacaca aaaatggcac acaaaaggcg tagacgctgc gtcagatcac tacaaacaat
     8581 ttagtattca agatccaatt gaagctcgtt tgacatataa agaaggttct gcgcaagtaa
     8641 tagataagag taaaggaaaa gatattactt ctgaaggcac actaacctat gatagcaatt
     8701 caagaacgct gaagtgggaa gcttccgctg acttcttaaa taacaatctt ttagatggac
     8761 gagaaattca gctgatattt acagctaaaa ctccattaca atcagaaaaa aatattgata
     8821 accaagccgt agtagcagta gataatgttt ctaataaaac aaatgttgtg acaattgggg
     8881 ttgatcctaa cttgccacaa gttattgttc ctaaaacagg ttctacacat ttagtaacga
     8941 ttttagcggt cagcttacta ttacttgtgt tagcaacttt tggctacgca gttctgaagt
     9001 tcaattaaac aaaaaaacaa ggctgaaaat cagccttgtt tttctatttg aaaatgggat
     9061 tggcactaat aaatacgttg tctgaagaaa gtgatccgtc tgcagaaact tcataagcat
     9121 gggataaacc aacgatactt tgattgttgt aaagcaagtt tgcgttatga gcagcgctat
     9181 ctttccacat aaccatagca tttactgctc ctgtatctga aaggatctcg tcaacgtaaa
     9241 ggtagaaact attgatattt tctcctttag caccgtagca gcctaagctt tctaagagat
     9301 cttggaaagt accgccgcca ggtcggtaat gactgaagct tacttgtaat tcttttgcac
     9361 gaatatctgc agcttgttga acgatatcat tgaattgcat tggggcaacg ccattttgtt
     9421 gacggtagct atttaaccaa gcaagttctt tttgacgcac atagttgttt aaatcactta
     9481 gtgagatatt tttgtatttg aaagtgatcg ttcgattttg gtcaacatta ttgattacac
     9541 gttgtccatt tttatcaacg gtaacagatg aatcgtcatt tctacacaag aagtagtttt
     9601 gatcggtcat atttttccaa gaagtagaga aactatctcc ctcgtaaacc gtttgtgttt
     9661 cagtagataa aacagcgttt gtgtcaacgt ttatatggtt cacagtgacg ttgtattgat
     9721 gagtataggc aaaattgatt ccttgattac ttgtaacgtt gtttaaagtc acagtgttgc
     9781 tagagctttc tctaggatca agaacaaaat tagtgaacat aggtgcttca attgtgatgc
     9841 tttgtccata aacgacggga taactcttac tgtaattata gagttcttgg atatttggat
     9901 cagtatccga gactttattt ggacgagctt tcacttcaac tagaaagact tcatcatagt
     9961 aaaaagtaaa tgtggtattt gtgttaatcg tttgtgtttg tgtttcattc ccatttactt
    10021 gatactgtgg tgcttcaaag ttagtcaagt cggttctagc ttttgcagtg taacttgtac
    10081 cagcttttac tgagacatgt tcttctttta gcagcgttcc agtttgtcga ttcatatgac
    10141 ggacagttac cgcatattct gaaggtttag gtttgtgttg taacccttta aatgcagaat
    10201 caagactatt tttagcatta tttacttggg tttgagaagc tttagaatcg tctagtactt
    10261 ttttagcatt ggataaagca gtctgaaaat tattccaaga tccttctgta aaatctcctt
    10321 tagcagttga ttttacttgg ttgtacaagg cttgtaattc cgttttgttc acagtaggag
    10381 ctggtttttt ctttaatccc ttgtaagcag aatctaatgt gtttttggca ctatccactt
    10441 gtttttgagt cgcttttgag tcatttaaca cttttttagc attgtttaaa gcggtttgga
    10501 atgcgttcca ggtattgtcg gtatagtcac cttttttagt tcccttaacg ctcgtataaa
    10561 gagcttctaa agctgattta ttcactttgg gtttagaggt gtcagcacca taccaaccaa
    10621 taccttcgta tttccagcca tagtctttgg ttaaccattg tacttctttc caacttgcag
    10681 tgtagtgatg cgaaccagag gtaacacctg gaaggaaaag gcggtagatc ggtttattat
    10741 ttttttcggc agaacgccag gcaggccctt cataagtcca accgtaattt ttggtcaacc
    10801 atttcacttc atcccaacta gctgtgtaat ggtgatcacg aaggccttta ttgtataaac
    10861 gatagacaag gtggccaccg ctagtcggtg cttcccaagc ggggccttca taattacccc
    10921 acttaatttt cactaggtga tcacgttctt ttgtgctaga ggtgtaaaaa tgttcatggt
    10981 tatttggatt ataaaggcga tacatgacgt tagttgaagc agcgtcagca gatccagcaa
    11041 gagcaactgt ggcagcaaat gttccaatag ctataacagt ttttgggttt aataatttca
    11101 tgttttcaac tccctattaa atgattaaat attttgttta cattcataat ataccacggt
    11161 ataccgaggt agtcaacggt ataccgtgga aattttgaaa taaaaaagcc gccctatgaa
    11221 gagtgtaagg atggctgttt atatgctatg aagaaatgga ttcggcgtat ttggttagtt
    11281 tggcagcatt atcaaatcgc attttttcaa tcgcactttt tttctttatt aaatccgcaa
    11341 ttgttgattc tgcgactgaa gaatgtttgc taatttcata tgctgtcttg ccagtttcaa
    11401 atagtaacca ttcgattttc tctttatcag cgtacattgc aatgctccct cttgattttt
    11461 tctttattat agctcttttt tgtaataaat cacaatatca agaggaaaag tttctataga
    11521 aaggtgggaa attatgagta tccgttcccc gtgtatcaaa atgaggtttg ttgctacttt
    11581 cgttatttaa tatactaaaa taaaacaacg aaagaaggaa tagaatgaaa aagttaataa
    11641 tggtatttac ttcgttgatt gccatgattg ttattttagg tggatgtggt agtgaaaaaa
    11701 ataagcaaga tagtcaagat aatggcgtgt taaaaccagg aacagtgtgg aaagaagaag
    11761 taggcggact tgttcataat ttaaaaatta tagatgatac aacatgggaa tattccgaaa
    11821 gtgtttggca tcctgaacaa gtacaaataa cagttgagaa gctaaaagac tataaaggat
    11881 tagaaagata taaggtaatc gattcaggtg gagtagattt attcataggt aaaacagcct
    11941 taatagttcc ctataacaaa gacggtttag aaacgtttaa atttattcca acaaacgaaa
    12001 aagaacatcg agataaaagt gaaatattaa aagaatacag tactaaatct ggatacaagt
    12061 tacaaaagac tagcgagtga ttgctagtct ttttatttat cctgaaggag gaatcgcagt
    12121 ggcaaaagc
//
LOCUS       DACDYJ010000075        12106 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDYJ010000075 DACDYJ010000000
VERSION     DACDYJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.075"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            24..695
                     /locus_tag="IWM51_002241"
     CDS             24..695
                     /locus_tag="IWM51_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9971637.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            754..1194
                     /locus_tag="IWM51_002242"
     CDS             754..1194
                     /locus_tag="IWM51_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971638.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1206..1874
                     /locus_tag="IWM51_002243"
     CDS             1206..1874
                     /locus_tag="IWM51_002243"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9971639.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1938..2561
                     /locus_tag="IWM51_002244"
     CDS             1938..2561
                     /locus_tag="IWM51_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP9971640.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2617..3003
                     /locus_tag="IWM51_002245"
     CDS             2617..3003
                     /locus_tag="IWM51_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP9971641.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3216..4607
                     /locus_tag="IWM51_002246"
     CDS             3216..4607
                     /locus_tag="IWM51_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP9971642.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            4954..5601
                     /locus_tag="IWM51_002247"
     CDS             4954..5601
                     /locus_tag="IWM51_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9971643.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            5994..8639
                     /locus_tag="IWM51_002248"
     CDS             5994..8639
                     /locus_tag="IWM51_002248"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP9971644.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            8949..10262
                     /locus_tag="IWM51_002249"
     CDS             8949..10262
                     /locus_tag="IWM51_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP9971645.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            10252..10905
                     /locus_tag="IWM51_002250"
     CDS             10252..10905
                     /locus_tag="IWM51_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9971646.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            10960..11652
                     /locus_tag="IWM51_002251"
     CDS             10960..11652
                     /locus_tag="IWM51_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP9971647.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            11802..12002
                     /locus_tag="IWM51_002252"
     CDS             11802..12002
                     /locus_tag="IWM51_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9971648.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         4012 a         2034 c         2623 g         3437 t
ORIGIN      
        1 atgatgaaaa aagggggaga aaaatgaatt ttttagttgt tttaaaagat actctgattg
       61 agcgatctac atttctatat gttcaattgc aggaaaataa aacattgctg catttttcac
      121 cagagttcca tctagagggg ctaacattag gagaaatcta tcaagcagaa gggctgtcgg
      181 ctgttttgca cttttttaag gtagaattgg aattaccagt aaaagattat atcgagcttt
      241 ctcttgaatc ttgggatcga gcagtggttg aactgtttcc agaaggttta atgatcgata
      301 caaacgatgg gaagatccat ctgactgcag cagaactgca aaaacaaatg cgttatcaaa
      361 tagatgacga aaattctttt tctattttta gacgtcagca aaagattcta aaaagctttt
      421 taaaagtgat cagtaaaaaa cggaatctga tcaaaacgac acagctgttg aaaaaatatc
      481 caggtctgct tcacacgtct attcaatttc ctcaattgat tacgatgatc catcgtttta
      541 tacgtatgca acagctacca tctaagaagc tgtttcttcc attgacggac acatttcgag
      601 tagtcaacag agaagataaa aagaaagtga ttgtgattga ctttaccaga aatcggaatg
      661 tccttcacca agttgcaatg gaaaaggcca attaaggctt tttctttttg gatttttttg
      721 tcataataaa tattagatga tggaggagat aatatgatgc aaacatggtt agaaaggcta
      781 atacgatttc agcagaacta tcacaaactt atgcagggaa gatatgcgaa atttgatcag
      841 ttgaatcgcg gtctattgat cgctgcgctt gtctttagtt tgctaagtcg ttggttgccc
      901 tatagaatcg gacaattgtt gttcatcgtc tttttcggat ggatgatatt tcgtttccta
      961 tcaaaaaaaa tctaccccag actaaatgag aatcaacgtt atttgaaata tcttgataga
     1021 gtcaaagaaa aatatcatca gataaaaaac aaaaagcaag tcaaaatgga tcgaaaaagt
     1081 tatacttttt ttgagtgccc gaattgtcat caaaagcagc gtgcacctaa aggaaaagga
     1141 cgaatacgtg tgacatgcaa aacatgtggt ataaaattcg aaacgaatgt ttaagggaga
     1201 aaagaatgaa attagtatta ttacgtcatg gcgaaagcga agcaaatttt gaaaattact
     1261 ggacaggatg gctagatgtc gccttaacag aaaaaggaaa agaacaagca gcagttgccg
     1321 gtcaaaaaat gcttgaagcc ggacttcaat tcgatgccgt ttatacctct gttttgaaac
     1381 gtgcgattct taccggacaa gtcgcactag aagaaatggg tcagttatgg cttcctataa
     1441 taaaaacatg gcgtctaaat gaacggcatt atggggatct tgtgggtaaa aacaaagata
     1501 agatgaaaga aatatacgga aaagatcaag taaaaacatg gcgcagagga tatacggaag
     1561 ttccgccgtt aaccgcagaa aatagatttg accgcagata tgatcacttg gatccgcatt
     1621 tgatcccata cggagaaagt ctggaaatga cagtgaatcg gatcattccc ttatggcaag
     1681 atcatctagc accaaaacta aaagatcggc aagatttact agttgtgggt catggtaata
     1741 gcatccgagc actgacaaaa tatttagaag atgttcctga agatcaaatg gatacgatcg
     1801 atatcccaaa tgcacaacca atccagtata cattagcaga tgatttgaca attctggaca
     1861 aaaaaatact ttaaagaaca cctttatcta aaagtgagag atagaaaaaa gaagcaaagg
     1921 aatggaacaa aaggaacatg aaaaattata tatgggactt tgatggtaca ttgtttgata
     1981 cgtatcccgc aatggtagat ggtgcatggc aagcactaaa agattttggt atcagtatgg
     2041 acaaaaaaga aatctacttc aaaatgaaaa agtattctac tagttatttg atcaatgaat
     2101 caaacttgaa tgccagagag tttaatgaat tatttcaccg atatgaaaaa gaaagtacgg
     2161 aagtttcccg tccatttcca gaaacgaaac aagtactgga aatgctaaaa gacaacggcg
     2221 gtcgtcattt catcctgacc caccgtttga cagaatcaac atggggcctg ttaaaagaac
     2281 atcgattggc acatctgatt gaggaagtag tagggatcga tcaggacttt cctcgtaaac
     2341 ctgatccagc atctttaaat tatttgatcg atacttttca tttagaaaga acagacacga
     2401 tgatgatcgg cgatcgtcgt ttagatatcg aagcagggaa aaatgcagga gtcgctactt
     2461 gtctttacga catcgatcat tttctgggtg agatcccggc agattatgtg gtaggaaatc
     2521 tgaatgaaat ccttaccttg cagagcatca ttaaaaagta gtatcccctt tcaaaggaga
     2581 tatgttataa ttttcataaa ggagtgataa aattatatgg aaatcagaag acaaaaagaa
     2641 gttgtagaag catttcatta tgatatgaga ttgccgaacc aagaagtaga aacagatttg
     2701 aaagtaggct tctcgccttt aaaagaaacg atcgaaaact atccgaaaga acatagcatc
     2761 gtaggagcac gcttagagtt ccgtttagtg tttgatacat atgttttatc tggaagtgtg
     2821 agtcagatca atcatatcct caatcggaaa attgaaggtc aaaatgatgt cacacaagaa
     2881 gaagtggacg agctagtagc accgttattc gatatcgtac gccgaatgtc ttatgaggtg
     2941 acagagatcg ccttagatca gcctggggta aaattgaatt ttcaatcaga aaaacaagaa
     3001 tagacagtaa aaacatacct gcgatagatt aaagctatca caggtatgtt tttttattac
     3061 taattgtaag caaaaagaat gtcatttcga taaaaatcgt ctaaaatata aaagtcactg
     3121 aaaaaaattt tttcaggagg atggttgaag aatcattctc gggaaccttt acttccttcg
     3181 atggacttgt cattaataat atgaggagga gaaaaatgac aacactcatc acagttttag
     3241 tggttctagt ttttgcggca ttattatatg tgttctatcg tatgcaaaag cgccatacaa
     3301 aattttcaac acgcgtcttt acagcattag gagttggtat cgtctttggc ggtctgcttc
     3361 aattgatctt tggtgtagac gacaaagtcg ttactcaatc aatggaatgg atcgggattg
     3421 taggtcaagg atatgtatca ttcttgcaaa tgctagttat gcctttagtg tttatttcga
     3481 tcattggtgc attcacaaaa atgaaagttt ctgaaaagct agggaaaatt agtgcgactg
     3541 tattgacttc tttacttgga acaacagcta ttgcagcatt gatcggaatt ggttcaacaa
     3601 tgctgttcgg actccaagga gctaaattca cacaaggagc tgcagaaaca gcacgaatct
     3661 ctgaactggc aacacgtcag gaaacagttg aaaacttatc gattccacag cagattttat
     3721 cctttatccc taaaaacgtt tttgctgatt tagcaggtag tcgtgcaaca agtacgattg
     3781 gagtcgttat tttcgcggca tttgtcggaa tcgcttatct aggcgtgaaa agaaaagcgc
     3841 caaaagaagc agcattcttt tcagatttag tcgacagtct gtataaaatc attatgcgga
     3901 tcgtgacgct tgttttgcgt ttgacacctt atggagtatt tgctttgatg acaaacgttt
     3961 tggcaacaag tgattttgca gctatcgtca atttaggtaa atttgtcgtt gcttcttacg
     4021 tagcattgct tgctgttttg attgtccact cactgatttt gattggtgca aaagtcaatc
     4081 caatcaacta tttcaaaaaa gcctttccgg tattgagctt tgcgtttact tcacgttcaa
     4141 gtgctggagc tttgccattg aatatcgaga ctcagacaaa agctttagga gtagatgatg
     4201 caacagctaa tttctcagca agttttggtt tgtctattgg acaaaacggc tgtgctggcg
     4261 tttatccagc tatgttggca acgatcgtcg caccagcagc tggagtgaat gtctttagtt
     4321 tggaatttat tttgatgctt gtggcaattg taacaatcag ttcttttggt gtagctggag
     4381 tcggtggggg cgcaacattt gcttcactga tcgttttagg ggcaatgaac ttgccagtag
     4441 caatcgttgg gttagttatc tctgttgaac cattaatcga tatggcacgt acagcagtaa
     4501 acgtcagcgg tagcatggta gctggtatcg tgacaagtgc acgcatcaaa gatttagatc
     4561 gcgatgtcat caatgatgat tctaaagtga tcgatgtgaa tgcatagtca aaaagaggtt
     4621 gtgatagaag tggtcagctt taagaaataa gaagaagttt cctaaaaata gtttctcaaa
     4681 tttttgtgaa atttcggctt atttccgaga agctacttct gaaacaccgt gaataagagg
     4741 ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat
     4801 attttacgtt tggttgagct tattaccgca gatgcaagct tttgaacacc gttttatttg
     4861 tctcacaccc ttttccttcc tacttgtaac cttgctcccg tgttagaaac atgttacact
     4921 aaatatgtga aattattaac aaggagcgtc gttatgcgag aaaaaacaga tacactgcca
     4981 aaagcaactg cgaaaagatt acctttatat ttacgctact tgaagatgct ggatgactca
     5041 ggtataagta gaatcaaatc aaatgagttc agcgaaatca cccaaattcc ttctgcaacg
     5101 atccgcagag acttctcgca attaggcgag ctaggcagaa gcggatatgg ctatgatgtc
     5161 ccttttctta tcgatgtatt caataatatc cttaatacta aagaagaaaa acgaatcgca
     5221 ttagtaggat atggaaatct tggcaaagca ttgaaacaca acaattttag aagaaatgaa
     5281 aatctaaata tcgtgtgcgt ctttgataat gatccagcac tgatcaatcg agtaattgat
     5341 ggagaaatga tctatccgat tgatcgtttc gcggagatag ccaaagcaaa aaatgtgaca
     5401 gtagcaatct ctacggtacc aagtaaatat tcacaatctg ctattgatga aattgtcaaa
     5461 ggaaatgtaa ccgctatact taattttgcg cctgacagag taactgtccc agcttatgtc
     5521 aatgttcaat acattgattt gacaaccgag ttgcagacgc ttatttactt tgatgagaat
     5581 tactcagaag ttttttctta ggaaaatttg agtaggctta ttgatcttgc gagaactcgt
     5641 cgtaattctt tcaaaaattt tttagtacta aatgaagtgg ttttttcacg accacttgtt
     5701 gactaaaaaa aaaaaccatt gtagaatgaa gcataacgct gaacaagagg agtaactggc
     5761 acattcattc ttagagagag aactatctgc tgaaaggttc tcaatgacaa ccagtgaagg
     5821 tagcttggga gtttctgtct tgaatggagt agagatagac gagtcgtgat cgttaactca
     5881 cttgagaggc aataatgatt gattattgca atttaggtgg taccgcgtaa atcatacgtc
     5941 ctagaatacg aaagtatttt aggacgtttt ttagtttaaa ggaggaaaac agcatgacgg
     6001 aagaaaataa cctatcaaca aaatataatc cgcaagaagt tgaagctgga cgttatcagc
     6061 attggttaga acaagatttg tttaaaccga gcggagataa aaaagcaaaa ccttattcaa
     6121 tcgtaatccc gcccccaaat gttactggaa aacttcattt agggcatgct tgggatacga
     6181 ccttgcaaga tatgctgatt cgtcaaaaga gaatgcaagg atatgatact ttatggctgc
     6241 ctggtatgga ccatgcagga atcgcgacac aagcaaaagt agaagcgaag cttagagagc
     6301 aagggatttc tcgttatgat ctaggtcgtg aaaagttcat cgatcaagtg tgggaatgga
     6361 aagaagagta tgcgagccac atccgcgaac agtgggctaa aatgggctta tctttagatt
     6421 acagtaggga acgttttaca ttagatgatg gattatctga agcagtacgt aaagtttttg
     6481 ttaccttata cgaaaaaggt ttgatttatc gtggtgaata tatcatcaac tgggatcctc
     6541 aagcacgaac agctctatct gatatcgaag ttattcataa agatatcgaa ggcgcctttt
     6601 atcatatgag ctatgaacta gcagatggat caggcgtagt agaaattgcg acaacccgtc
     6661 cagaaacgat gttgggagat acggcgattg ctgttcatcc agaagacgag cgttatcaag
     6721 cgctcatcgg caaaaaagtg attctacctt tagtcaataa acaaatccca atcattgcag
     6781 atgaatatgt agatatggaa ttcgggacag gtgtagtaaa aatcactcca gcacatgatc
     6841 caaacgactt tgaggtaggt aatcgtcatg atcttccacg cgtaaatgtt atggatgatg
     6901 atggtacgat gaatgaacta gcaggaaaat atgctggaat ggaacgtttt gctgctcgta
     6961 aagcaatcgt gcaagatctg aaagaaatcg gtcgattgat taaaatcgat acgatgatcc
     7021 acagtgtggg tcattccgag cggacaggtg tcgttgttga acctcggctg tctactcaat
     7081 ggtttgtaaa aatggcgccg ttagcgaaaa aagccattga taaccaagaa acagatcaag
     7141 cagtggaatt cttccctcca agattcaatc aaacgttttt acgttggatg gaaaatgtcc
     7201 atgattgggt aatctcacga caattatggt ggggacatca aattcctgca tggtatcaca
     7261 aagaaacagg tgaaatgtat gtagggatgg aagcaccagc ggatagtgaa aactgggtac
     7321 aagatcctga tgttttagac acttggttta gttcagcttt gtggcctttc tcaacaatgg
     7381 gctggccaga tacggaaagc gaagattata agcgatactt ccctactagc actttagtaa
     7441 ccggttatga catcattttc ttctgggtaa gcagaatgat tttccaaagc ttggaattta
     7501 ctggcgaacg tccatttgag aatgtcttga ttcatggatt gattcgtgac gaacaagggc
     7561 ggaagatgag taaatcatta ggtaatggaa tcgatccgat ggaagtaatc gaaaagtatg
     7621 gagcagatgc tctccgctgg ttcttatcga atggttctgc accaggacaa gatgtccgct
     7681 tcagctatga aaaaatggat gcttcttgga actttatcaa caaaatctgg aatgcttcac
     7741 gtttcgttat catgaatatc gaaggaatga ccgtagaaga gatcgattta tctggagaaa
     7801 aatcagtggc tgaccggtgg atccttactc gtttgaatga aacgatcgaa cgagtaactg
     7861 agctatttga tcgttttgaa tttggtgaag caggtcgaca actgtataac tttatctggg
     7921 atgatttttg tgactggtac atcgaaatga gtaaagaagt actttatgga gatgatgctg
     7981 tttcaaaaca aaccacccgt agcatcttag tccatacact tgatcagatc ctacgtttat
     8041 tgcatccaat catgccattc gttacagaag aaatatggga acatattcct catcagggaa
     8101 attctctagt agtggcagaa tatcctgttg tacgtccaga gtttgatgat gaaacagctt
     8161 ctaaagggat ggaagtattg aaagaattga ttcgagccgt tcgaaatatt cgttcagaag
     8221 tgaatacacc attgtctaaa ccaattacgt tgctaattaa aacaaacgat gctaaaatcg
     8281 atcaattttt agttgaaaat acaagctata tcgaacgctt ctgtaatccc gaagagttga
     8341 cgatatcaag tgagatcact gcgccagatc tagcgatgtc agctgttctg acaggtgcag
     8401 aaatcttctt gccgttagca ggattgatca atattgaaga agaaatcaaa cgattagaaa
     8461 aagaactgga aaagtggaca agcgaagtaa aacgtgtcca aggaaaactt tctaatgaac
     8521 gttttgtttc taacgcgccg gaagaagtag tagaggcaga gcgtgcaaaa gaaaaagatt
     8581 atttagagaa gcaaacggct gtcaaagaac gaatcgaaag tttgcgtact attcaataag
     8641 aatagaaaaa gagagtgtga aatcagctag ctattactaa aagctagctg attgaacatc
     8701 gttacaggaa agagtttgag cctcactctt agcttcgatt cataaggaga tgtgacataa
     8761 attttgtcgc atctcttttt tagtctactc ttattatgta ggagaaggag ggaggtggat
     8821 catgtatcaa gagtcaataa agtagttgat aaaatcgttc ctctctctgt ttcttttctc
     8881 tttgtcatac ttttattttt cttatgtgtt ctatttgtta taataaaaga gaaatggggg
     8941 aattgttgat gacgattgaa gaagcaatag aatggatcca tagtcgatta ccatttggtt
     9001 cacgtcctgg ccttgaccga gtcgaagctt tattagaagt ggtagggcat ccagagagaa
     9061 aagtaaaaac gatccatatc gctggaacaa acggaaaagg ttctactgta gcttatttga
     9121 gaagcctatt agaagaggct gggctgacag taggtacatt tacttcacct tatatcgaaa
     9181 cgttcaatga acggatcgca atcaatggca aaagcattcc agacaatgat ttgatccagc
     9241 ttgtaaaaaa atatcagcca ctgatcaaac aacttgatca atttgaagca gtagctggta
     9301 ttactgaatt cgagacatta acagcaatgg catttgatta ttttttgatg aagaaagtgg
     9361 acgtagcgat tatcgaagtt ggcttaggcg gcttattgga ttctaccaat gtagctgtac
     9421 caatgctgac gggaataaca acgattggac ttgatcatac cgaaatactt ggagatacaa
     9481 ttgaagaaat cgctgcacaa aaagctggaa tcatcaaaca aggcgttcca gtagtaacag
     9541 gaaatattgc cccagaagct tttactgtca tccaacaaac agctaaagca aaaaatgcac
     9601 cgctttatgt atacggggaa aattataaaa taaactatct gcatcctgat gagatgtggg
     9661 gagaagtatt tgaatttttt aatgaaatag gaaagatccc tcaattaacc actccgcttt
     9721 tagggcaaca tcaagctgaa aatgcaggag tagccatcga attgtatttt gtttactgtc
     9781 aattggaaaa gctgcctttc cgagaaaaag atgttttgaa gggattgaag aaatctcgat
     9841 ggccaggaag aatggagcgt ttgagtaacg agccattagt tgttttagat ggtgcccata
     9901 atacgcatgc aatgaaccgg cttgttgaga atttgaaaaa agaattcaaa ggatatcaaa
     9961 tcaacatttt gttttccgcc ttacgaacta aaaatgttag agaaatgcta gaacgattgc
    10021 agcaagtacc aaatgtccat atttatttga cgacctttga acatccaaaa gccatcgaat
    10081 taagtgaagt atcagattta gcaagtgaga gagtatcaat cgtttcattg tggcaattcg
    10141 gattagcaga tatattggaa aagatgacaa atgaagacgt attagttgtg accggttcac
    10201 tttatttcat ttcagaagtt cgccatttat taatacaatt aggaggagat catgaagtat
    10261 aatgcagtga tttttgacat ggatggtttg ctgttcgaca cagagttagt gtattacgaa
    10321 gccacacaag tagtagcaga caagatgggt tttccttatg ataaagacct gtatttaaaa
    10381 ttcttaggtg tttcagatga agaagtatgg gcgaattatc atatgatttt ctcggaattt
    10441 ggcaaaacaa gtgtacaaag attcatagat gattcatacc aggaaacgct tgaacgtttt
    10501 tcgcaaggag cggtacaaat gaagcccggt gtgttggaac tcctctcttt tttagaagaa
    10561 catcagattc ccaaagttgt cgcttccagt aaccaacgag cagtcattga acttttattg
    10621 aagaagaaca atctgtttga acgatttgag acgattgttt ctgcagaaaa tgttcaacga
    10681 gcaaaaccag acccagaaat tttcttgtta gctaatcaga agctaggtac agcgaagaaa
    10741 gaaacattga ttttagaaga ttcacaaaat ggtattttag cagcttctgc ggctgagatc
    10801 ccggtagtca tggtaccaga tctgcttcca ccatcccaag aattgatcaa aaaaacagct
    10861 ggggttgttt cttctttaca tgaaatccct gcattgttaa aataaaaatc catctctttt
    10921 cgtatcttat tttagataag atagaaagga gatgtttttt tgggaaaacg attgatcaaa
    10981 gaagtaccta ctagctcttt accaagggaa cgtatggaaa tttatggcgc ggaagcttta
    11041 tcggatcagg agcttttggc aatcttactg cgtaccggtc aacaccctta tagtgtgatg
    11101 tcgattgctg gaaatctgtt aaaaacgttt ggaggactgg cctctttacg acaggctact
    11161 ttgcatgagt tagaagagat tcgagggata ggacgcgtga aagcaatcga aatcaaagcc
    11221 ttgatcgaat taggtcgacg aattcagacg gatcatgaaa gagcggctcc accggttcgt
    11281 accagctacg aattggccca gcaattgatt ctggaaatga aagatcacaa acaagaacat
    11341 ttggtttgta tttatttgga cacgaagaat caagtgatct taaaaaaaac agtgttcatc
    11401 ggttcgctga atcagtcagt cgctcatcct agagaaattt tccattatgc tgtacgttat
    11461 tgtgcagcaa ggatcgtttt agcgcataat catccttctg gtaatgttat tccatcgcag
    11521 caggatatga attttaccaa aaggatacaa aagtgtggag aaatgatggg gatcactgta
    11581 ctggaccact tgatcatcgg acgcaaacga tattttagtt tgagagaaga aggaatgatg
    11641 gaagagaagt aagttgcttt cttgcaaaaa aaatgaagca agtgtacaat tatattgtaa
    11701 ttttgtttgg gaatagaagt tgaaacatct gttgcacctc tatcgtacac gagcaatgtt
    11761 acaatttaaa ttagtgcttt tggcacgagg aggatattat tatggaaaac ggaacagtaa
    11821 aatggttcaa cgctgaaaaa ggatttggat ttatctcacg tgaagatgga agcgacgtat
    11881 tcgttcactt ctcagctatc caaggtgacg gattcaaaac attagaagaa ggtcaagcag
    11941 tgactttcga cgttgaagaa tcagaccgcg gcccacaagc tgcaaacgtt gtaaaagctt
    12001 aattttatct gtaaatcaaa ttgatggtag acatcaattt ttacgagaga acaaagcgag
    12061 taacaaaaat acctgccaca agagacaatt tctcttgtgg caggta
//
LOCUS       DACDYJ010000076        11824 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDYJ010000076 DACDYJ010000000
VERSION     DACDYJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.077"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..536
                     /locus_tag="IWM51_002253"
     CDS             <1..536
                     /locus_tag="IWM51_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9971649.1"
                     /translation="DYPRINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGS
                     QWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPD
                     LPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDK
                     LLNRIDKEILKVGNIDE"
     gene            529..1956
                     /locus_tag="IWM51_002254"
     CDS             529..1956
                     /locus_tag="IWM51_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP9971650.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            1958..3028
                     /locus_tag="IWM51_002255"
     CDS             1958..3028
                     /locus_tag="IWM51_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971651.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            3015..4436
                     /locus_tag="IWM51_002256"
     CDS             3015..4436
                     /locus_tag="IWM51_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9971652.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            4449..5456
                     /locus_tag="IWM51_002257"
     CDS             4449..5456
                     /locus_tag="IWM51_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9971653.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            5468..6472
                     /gene="neuB"
                     /locus_tag="IWM51_002258"
     CDS             5468..6472
                     /gene="neuB"
                     /locus_tag="IWM51_002258"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP9971654.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            6469..7617
                     /gene="neuC"
                     /locus_tag="IWM51_002259"
     CDS             6469..7617
                     /gene="neuC"
                     /locus_tag="IWM51_002259"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP9971655.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            7590..8267
                     /locus_tag="IWM51_002260"
     CDS             7590..8267
                     /locus_tag="IWM51_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9971656.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            8257..9399
                     /locus_tag="IWM51_002261"
     CDS             8257..9399
                     /locus_tag="IWM51_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP9971657.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            9412..10389
                     /locus_tag="IWM51_002262"
     CDS             9412..10389
                     /locus_tag="IWM51_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9971658.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            10389..11297
                     /locus_tag="IWM51_002263"
     CDS             10389..11297
                     /locus_tag="IWM51_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP9971659.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            11298..>11824
                     /locus_tag="IWM51_002264"
     CDS             11298..>11824
                     /locus_tag="IWM51_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP9971660.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTP"
BASE COUNT         4292 a         1454 c         2090 g         3988 t
ORIGIN      
        1 atgattatcc tagaattaat aggaataaca caactgcatt taaaattttt agagctataa
       61 ttaatcgttg tctgaggatg attaaacttc ccccaaaaat tagacaccat aaatttgatt
      121 tgtatcatgg ttctcaatgg tgggctttgt cgaaagaatg cctaacagaa ttaatacaaa
      181 tgtatgaaca aaatcaacaa gactacctga attttaaaat aggtatgttt gccccagatg
      241 aaaaattttt tcatacactt ttttttaatt cctcgttcaa aaataaaaat gttattggag
      301 gaccagattt accacttgaa ttgaaaaata ttgaagaaac tactttacaa acttcgaaat
      361 tagcaaatat tcatattatt gatccttcca tgaacaaatg gtttgatgaa agggatatag
      421 atcaaataaa agaaagtgaa aaattatttg taagaaaagt taaatcggat tactctgata
      481 aactattaaa tagaattgat aaagaaattt tgaaagttgg aaatatagat gaatagaata
      541 gtacctgttt tagagaagag tttattaagt cgagatgcgt ttcgagtaat aaatttaatc
      601 atttgtctgc tttattttat gcctagtact tttaatgtaa ctaatatccc gatgaaaata
      661 gcttttgctt ggggtggaat tatagttttg tatgaggttt ttataaagaa aaattttttt
      721 aaaatgcgtt tttcttggct actgattgga gcaatgatta gttttatctt aactataatc
      781 attaacagag aaaatttttt aattccatct atttataatt ttggatattt gctgattact
      841 ttgatagtaa tttttcctgc agacttttca caaagtgaag aggaaaaaaa gaaaaaaatg
      901 gtgtatttta ataatgtttt cattatcata atttttttag ctgcgctaat atctatctat
      961 caatttattt ttttgatttc ttatcatgtc ccaacaggaa cgccaggatt acttgcaaga
     1021 caaggtttta ttgaacatcg gttatttggt gtctatacaa gtccaaatgt tggagctatg
     1081 tttggatata cttctattat tttaagttta attagtgtat taatttcaaa aataacaaaa
     1141 acgattcgtg ttttttatat gatgaatttt atcgtacaaa tattttatat tacactttct
     1201 tcgtctagag gtgcccaggt agtaatttgt tcttttttag tattatttct tttgttgttt
     1261 gccaattcaa aatttagaaa gagcattaga gaagtactac ctgtaaaaat caaatggatg
     1321 gtaagcatat tattagttct attggtatat ttttcttttg gtactaatta tgctaaggat
     1381 tttttagcaa caattcctgt atcgattgat agaaaagtag aagttataga gtcaaatgaa
     1441 tcagataaaa ctccacttcc agaaaaagaa gtaatattac agcattctag tgataactcc
     1501 gaaatttcag ctggtcgttt tacgatttgg aaagctgcgc ttaaagttat aagaccaact
     1561 ttgttatttg gttatggaga gaccgatttc tatgggaata gcgatgcaaa atttttgaaa
     1621 aaagtagatc ttgatactac agatgtgaat gaactaaaaa gagcacatgg aaacatgcat
     1681 aatggatttc ttcaagtact tgtatcaagt ggtataattg cttttatatg tatctatgct
     1741 ttctatgtgt taaatatctt tagtttactc aaaacatttt ttagaagtaa agtgaattat
     1801 gcgtttcttg gtattgtttt aatttttata ctatctattt ttatagatga aatggtggag
     1861 gctcatgtac ttttcaataa gcgcgatgtc atttcaattg ttttctggta ttatttggga
     1921 agcatttctt acttgtattt aaaaaaggag aattaatatg aagacagctt tcattgcttg
     1981 gactccttta catttgatta atatattaaa tgtttatcaa aatttttacc gagaatcaaa
     2041 aggagattta tttatttatt ctgaatttga aaacgctaga agatatagtg agcgtataaa
     2101 agaattaggg ctatttaatg atgttgtttt tgtgaatcac cggaaaatcg gttcttcctt
     2161 taaacgtaaa attaacttgt taactaacca taatcaattt ttctcggatg atttatccgt
     2221 ttatgatgaa atttttatac agggggaaaa ttatttcaca aaaatacttt tctccaatgt
     2281 ttacaaaaaa aatccagatg taaaactaaa ttatatagaa gatggtttgg gtacttattt
     2341 gaagaagagt atattttcaa caagtaaaaa cgttcaaaga gtttttaaaa tattaaataa
     2401 aaatagcatc ttcaaaaaaa aaattgacga ttattttgtc tattgtccgg aattgttact
     2461 ttatgatgta aaaaatataa aaaaaattcc caaaatagat gaaaacagta aattgatgaa
     2521 aaacatacaa tcaatctttg ggatagaaga agtttttgtc ggagataaat gtatcttttt
     2581 tgatcaacct ttattatctg atggttatgg catagatgaa acagatattt tcgctaaagt
     2641 aaatagtata ttttctggaa aaaaagaaac tttaattaag cttcatccta gagcaaggac
     2701 aaatcgttat ggtgaaaaaa acatattgaa ttccagttta ccgtttgaag tcttgctact
     2761 tgaaaatgac ttctcttcaa cacttttgat tagtcctata tctacaattt ctttttcacc
     2821 atctatgatg tttgaaaaga cgatcaactc tattatgttg gctaaattaa ttttaaatga
     2881 atatacagca ttatctgaag ataaaaaaga aacattgaca aatattgttc ttttctgtga
     2941 acattataac cgtctatcag aagaaaaaat tttactccca gaaaactggg cagaattaag
     3001 aaagttggta gagtatggga ttatttaaca cactttccaa gtcaaaatta gttaagtcca
     3061 gtttctggta tactataggg aattttttta ttaagggaat ttcattcata actattccta
     3121 tttttgtaaa tattactact caagcagaat atggtttgat taataatttc acttcatttg
     3181 tatcaatttt ttcgattctt gttgggctaa gcctaaattc ggcaattaat aatgcaagtt
     3241 ttgactataa agatgacata aatgaattta tgtcatcggt actttctcta gctacacttt
     3301 cgttaattgt gttcttatta atagggaaca tatatttttt gttttcaaat gctttttttg
     3361 atatgaacca acttgtattt aatttgatgt tattacaaag ttttgctagt tttgtaatta
     3421 gttatatcca gtcttatttt acgattaact ttaaccattt taaatatttg attttatctt
     3481 ttatatctac ttttttgaat attgcaatct ctgtagcttt gatgcagaca ttatattttg
     3541 atgataaata tgttggcaga gtagtcggcg gaacactgtc atttgtgttg ataggtttag
     3601 taatttattt aagaataatg ataaagggtg ctcatttttt tgataaaaaa cagtggagat
     3661 acgctttaaa aatctctttg ccgctaatcc cacattcctt agcaaatata gttttagcac
     3721 aattcgatag aattatgatt aattcttatt taggtcctgc tgcgggggga atatattcct
     3781 acattagtaa cataggtatt attctaagcg taatatggat gtctactaat aacgcctggg
     3841 ttccatggtt ctatggtgag atgagtaaaa ataatgaaaa aaaagtgaag aaagttagta
     3901 actattattt agtagtcttt actttgatta cgatgattgt aatggtagtt tcgattgatt
     3961 tagcaaaatt tatggctccg aaagactatt attctgggtt atctttagtg gttccgataa
     4021 ccttaggcta tttctttcag tttttatata gtttaccagt taatgctgaa ttttatgaga
     4081 aaaaaacatc gtttattgcg attggtacag tagcaagtgc tatagttaat atactattaa
     4141 acatgatttt catacctagg ttaggaataa ttgctgctgg atacacaacc gtatttacgt
     4201 attttttact ttttattttt cattataata tcgctaaaaa aattacgaat aaacaattat
     4261 ttgacacaaa aaaaatatgt atcataataa tttttgtatc aattttttct ttgatacttc
     4321 atattttaat caatcaaatg ttgattcgtt acgttttgac atcattattg ataattagtt
     4381 tagttatata ttttttaaag attaagaatc gaattataga tgactataat tcataggagg
     4441 agatataagt gtttaaagga aaaacaatat tagtaactgg cggaactggt tccattggaa
     4501 gtgaaattgt tcgtcagctt ttaaaatatg aaccaaaagc tatcagaatt tatagtagag
     4561 gtgaagaaaa acaattttat atgcaacaag agctctcaaa tcacactaat ttgagatttt
     4621 taattggtga tatcagagac tctcaaagga ttgattatgc atgtcgtgat gtggatatta
     4681 tttttcatgc tgccgcaatg aaacacgttc cagcaagtga atataatcct atggaagctg
     4741 taaaaacaaa tgtgatggga actcaaaatg taatagatgc agcgatcaaa aataatgtaa
     4801 aacattttgt aggtatcagt acagataaag tagtgagtcc tactaacacc atgggtgcga
     4861 caaagttatt gagtgaaaaa ttaataatag ctgccaatca gtataaagga aaaacagaaa
     4921 caatattttc ttgtgtaagg tttggaaatg tcatggggtc aagtggatct gttattcctc
     4981 tttttagtaa acaaataaaa aacaataaag atattacttt gacagatgtt gatatgacac
     5041 gatttatgat gtcaataccg gatgctgcaa gattagttct gtctgctgct atgaattcaa
     5101 aatttggaga aacatatgtc ttaaaaatgc cggcaatcaa tattttaact ttagctcaat
     5161 cattaataaa aatatataat gatacacatg atgaaaaata tagaggtgaa ataaaagttg
     5221 taggtatcag gccaggagaa aaattatatg aagagctaat gactacagac gaaattgaac
     5281 gctcatatga aaatgataaa atgtatatca ttccaccaca ctatcatgct actgacgaac
     5341 tgtacaaaga ttatgaaaaa attaaaatca ctgcatactc ttcagatgca acagaattaa
     5401 tgagtggaca ggaatttatt agaatcgcaa aagagaataa tgtcgtgact tggtaaggag
     5461 ttttatgatg aaatcagaag tatatgtaat tgcagaaatt ggtgtaaacc ataatggaag
     5521 tctagattta gctttagaaa gtattgataa agctaaagaa tgtggagcta acgcggttaa
     5581 atttcaaaca tttaaaacga ataagctagt ttccaaaaag gctccaatgg caaaatatca
     5641 gaaggataat cttaaagagg acagttctca atttgaaatg ctaaaaaaat tagaattatc
     5701 tcaatctgat tttattagaa taaaagatta ttgtatacaa aataaaattg attttttgtc
     5761 tactcctttt gatgaagaaa gtgcagactt tctaaaacaa attggtgtac aaggatttaa
     5821 aattggctca ggagatttaa caaatttacc ttttcttaga aaaatagata tgtatgggtt
     5881 gccagtttta ttatctacag gtatggctga tattcatgag gtcagtgagg ctgtagattg
     5941 tttcatagat agtccagtta ctattttaca ttgtacttca aattatccgg ctagttctga
     6001 agatattaat ttattggcga ttaacacatt aaaagaaatg ttcgggatac ccgttgggta
     6061 ctccgatcat tcattgggat atgatgttgg tatttgtgct gttgcgatgg gtgctaaggt
     6121 aattgagaaa cattttacct tggataagga actacctggt ccagatcata aagcttcttt
     6181 agatcctcag gaatttgcag attttgtaaa gcatattaga aacactgaaa tatttttagg
     6241 tgatggtgtt aagcgagcaa tgccttctga acttagtaca aaagaagtat caagaaaaag
     6301 cctagtggtc actgagaatt taaatgtggg agatattctt acagagaaat ctcttacaat
     6361 aaaacgtcct ggaaatggaa tagctcctaa gtttttatat caatatattg gcaaaaaagt
     6421 taaaagacca ataagtgcag atagtgttct gcttgaggaa gatgttgaat gaaaaaaata
     6481 cttatagtaa gtggaacacg agctgattat ggaatatata aacctgttgc tgagaattta
     6541 ctacaagata attgggatgt atcatttgtt gtttctggaa tgcatttatc ccataaatat
     6601 ggatttacta aagaattgat cttagctgac ggatttagaa ttagtggaga agttcaaagt
     6661 ttgtttttag agaacaataa gggaaacatg gctcggtctg tggcattaga gataaatggt
     6721 ttcacaaata tatttgaaca aactagccct gattttgtat tattactagg ggatagagga
     6781 gaaatgttgg cagctgcgat agcttgtttg tatcttggaa tcaaaacagc acatattcac
     6841 ggaggcgaag tgtctggtac tgtagatgaa tcaataagac atgccataac gaaaatagca
     6901 tctgttcatt ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat gggggaagat
     6961 tcttggagag tttttcccgt cggagctcct agaatagata ctattttaaa taatagatta
     7021 cctagtttta gagatataaa agaaaaatat catctaaaat ttgaaaagaa aaaatacgtt
     7081 ttgcaagtat ttcatccagt tgtaaccgaa ctagaaaata tagaaaaaca aattttagct
     7141 ttgatcaatt caatggaaca acaatctcaa ccaattattt gcattttacc taatagtgat
     7201 gctggtagcg aaattatcag aaaaatgtac cagaaatatt taaaaacgga tattatttat
     7261 attacgaatt tagagccgga ggaatacctg actatattaa aagagtgcaa atttatgatc
     7321 ggaaattctt cttctgggat aattgaagct gcatcattca aaataccagt aataaattta
     7381 ggaaccagac aaaatggacg agaacaatct aaaaatatta taaatgcaaa tctaaatgaa
     7441 aatgaaattg aaactgcttt gaataaaata caagatcctc agtttataaa aagcttgaaa
     7501 aatgttaaga acatctacgg tgacggcaac agttctagaa gaataacaaa aacattgaat
     7561 caaatattga atgatgggga agatattaag tggattcaaa aacaaatagc ttattaaaat
     7621 atactagtga tccatacatt attattgggg ctggcggaca cgcaaaagtc attattgaaa
     7681 ccttaagatt agaaaataag ttaatatatg gattttttga tgataagcaa aataaaatgc
     7741 aagatctgaa atatcttggg aaaataaacg aattgaatag ctgggcggat agcaaaaaaa
     7801 aaatctataa atttattgta gctattggtg actgtgtagt aagaaaaaag attataaaaa
     7861 aattgaatat gccaagtgag caatatggaa tagctgttca tccaagtgct atagtggcaa
     7921 aagaaatttc aattggttat ggaactgtta tttttgcgaa tgctgtaatt aacccagatg
     7981 ctgtaattgg agagcatgct ataataaaca caggtagcat tgttgagcat gattgtagaa
     8041 ttaataacta tgttcattta tctccaggtg tatgcttagc gggtggagta catgtaggtg
     8101 taggaactca aataggaatt ggttctcaat gtatccagat gaagaaaatt ggatcttggt
     8161 gtatgatagg cgcagggagc actattgtta aggatattcc ctcacattcc ttagcttatg
     8221 gcaatccagc taaaattaag aaagaaggaa ttaattttga attctagaat tttattatca
     8281 agtcctcata tgggaggaac agaagaaaaa tatgttgcta aggcttttaa agataattgg
     8341 atagcgccat taggtgaaaa tgtggataaa tttgaagaaa gtattacaaa atatactggt
     8401 tctaagggcg gagcttctgc gttaagttca ggtacagctg ccatacatct tgccttaaga
     8461 ctgcttaatg ttggaaaaaa cgatattgtc tttgctcctt catttacttt tatagctaca
     8521 gtaaatccta ttttatatca aggggctgta ccagtgttca ttgactcaga acctaataca
     8581 tggaatatga gccctgttgc actagagagc gcattagaag aagctgaaag aaacaaccaa
     8641 ttacctaagg caatcatcgt agtgaattta tatggtcaaa tggctgatta tgaacgaatt
     8701 ttagctattg caaaccaatt taatgttcca gtgatagaag atgcagcgga atcgttaggt
     8761 tctacttata agggaatttc gtcaggctta tttgggacaa ttgggatcta ttcatttaac
     8821 ggtaataaga tcatcacaac ctcaggtgga ggagctctag tgtctagtga taaaaaatat
     8881 gttgagcatt caagattttt agcaacacaa gcaagagatc aagcaccgta ttatcaacat
     8941 agcgaaatag gttataatta ccgtctaagc aatatttctg ccggtatcgg acgtggacaa
     9001 atggaagtat tgaatgatag agtagctaaa cgaagagaaa ttttcaatcg atataaggaa
     9061 gctttttctg gaattgatag aattgaaatg atgccagaag tggatggcat ggtgccaaat
     9121 cgttggttgt ctactttaac gatcaatgat tataattcta ttgatcaagt agttaacgta
     9181 ataaataaac taaatgaaaa aaacattgaa acaagagctt tgtggaaacc actacatttg
     9241 caaccagtat ttcaaggggt aaaaatgtat taccatgata ataatcttag tgtgtcagaa
     9301 aagttattta atagcggctt atgcttacct tctggaagta atatgacaga tagtgaacaa
     9361 gaatttgtta ttgatgagtt taaaaaaata ttcaagtagg tgtgatattt tatgaacata
     9421 ggaattattg gtttaggttc catggggaaa agaagactta gactcattca agaacacttt
     9481 aatgatattt tactatgtgg tgttgatact aatgaggcga gacgaaagga agttgaaaag
     9541 gaatttggaa taccaactta taatagtcta acagaggcaa aaaatttgtt tgaaataaat
     9601 tcagtttttg tttgtacatc ccctataacc catgaattta ttataaaaga ggctttaaat
     9661 ttagattgta atatctttac ggaaatcaat ttattaaatg aatattatga tgaaataatc
     9721 gaagtagcta aaaagaaaga tagattactt tatctatctt ctacattttt gaagagaaaa
     9781 gaaattcaat atattaagtc taaagtgacg cataataaaa aaatttctta tcaataccat
     9841 gtaggacagt atttacccga ctggcatcct tgggaagatt atcgcaattt ttttgtttca
     9901 aacaaaaaaa ctaatgggtg tagagaaata ttcgctattg aattaccttg gattatagat
     9961 acatttggga aagttaaatc gtttactgta atgacccaga aattatccaa tcttgatatt
    10021 gattattcgg acagtttcac tcttattatt gagcatgaaa ctggtatagt tggaacttta
    10081 agtgtagatg ttgtttcaag agtagctaaa agaaatttaa ctataattgg tgaaaagatt
    10141 ttaatagaat gggaaggtaa cccggattct ctattttcat ggaatgaaga aacttctagt
    10201 atgacagata ttcaattgta cgatgaggtt atgcatgata ataattattc acatactatt
    10261 attgaagatg catatttaga ggagattaaa gaatttttca cgcttttgga aaagaaaatg
    10321 gaaccccaat atacttttga aaaagataag tatattatta acttaattaa cgacattgag
    10381 gtaaattaat gatgaaagta attttttttg ggttagggtc aattggaaag agacatctaa
    10441 aaaatctaat aagctatgga aaaaagaatt ctattaattt tgatataaca gcttatagta
    10501 gcagtggaaa aaaagagttt gaagaaataa cgtatattca aaatagagac gatttggata
    10561 cacattatga tatagctttt attacaaatc cgacttttaa gcatctggaa acactaaatg
    10621 taattgttga aaaagttgat tacatatttt tagaaaaacc tgctttttct gagagtattg
    10681 atcttactca atataattat gattacaaaa atatttatgt tgctgctcca ctgaggtata
    10741 aaaatatcat ggaagttgtt cgtaatagta ttcaaagtaa aaaagtatat tctgtaagag
    10801 ctatttgttc tacttatctt cctgattgga gaaaaggtac tgattataga aataattatt
    10861 ctgcctttaa atctatgggg ggaggagtag aattagactg tatacatgaa ttggattata
    10921 ttataaacct atttggtttc cctaaatcag taaaaaaaca gtttggtaaa tattcgaatc
    10981 ttgaaattaa cagtaatgat ttagcaacat atatattgag atacgatgat aaaattatag
    11041 aaattcattt agattatttt ggaagagttc ctacaagaaa atttcaattg tttactttag
    11101 atgaatgtat caatgttgac ttattaaata ataaaataag ttttgaagaa aacattgtta
    11161 aagaaatgcc tgaatctgct aatgatatgt atgagaagga gttgaaatat ttctttgaaa
    11221 acgtagtaac aggcaaagaa aactggaatg atttatatca tgcaaacgaa gtattgaaaa
    11281 tagctgagga gaaataaatg gaaattttat ttactatttg tggtagagct gggtctaagg
    11341 ggtttaaaaa taaaaatttg aaatatttgg caggaaagcc cctagtccat ttcgctttag
    11401 caatgataga tatctatcaa caaaaacatc ctgaacataa ttgcacgact tgtttgaata
    11461 cggacagtaa tgagttagta gaattgacga ctaagttaaa tccgtctgtg aaaattgtag
    11521 aacgtaaacc tgaattagct acagatactg tagggaaaat tgaagtgata cgtgattcat
    11581 tcttacaact agaaagagaa gataatatat ttgatttaat tatagattta gatttgactt
    11641 cacctttacg tgaagtttct aatcttgaag aattaataga tgaacatcta aaaaatttag
    11701 ataaagacgt ctacttttca gttgtttctg caagacgtaa tccctatttt aatatggtaa
    11761 aaagaattga aaatgaagtt actatagtta ataaatcaag atacacatct aggcaacaga
    11821 cacc
//
LOCUS       DACDYJ010000077        11720 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDYJ010000077 DACDYJ010000000
VERSION     DACDYJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.078"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(363..1481)
                     /locus_tag="IWM51_002265"
     CDS             complement(363..1481)
                     /locus_tag="IWM51_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971661.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1952..3259
                     /locus_tag="IWM51_002266"
     CDS             1952..3259
                     /locus_tag="IWM51_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP9971662.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3378..4577
                     /locus_tag="IWM51_002267"
     CDS             3378..4577
                     /locus_tag="IWM51_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9971663.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4604..5872)
                     /locus_tag="IWM51_002268"
     CDS             complement(4604..5872)
                     /locus_tag="IWM51_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP9971664.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6035..6681)
                     /locus_tag="IWM51_002269"
                     /pseudo
     CDS             complement(6035..6681)
                     /locus_tag="IWM51_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7089..7592)
                     /locus_tag="IWM51_002270"
     CDS             complement(7089..7592)
                     /locus_tag="IWM51_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9971665.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7715..8479)
                     /locus_tag="IWM51_002271"
     CDS             complement(7715..8479)
                     /locus_tag="IWM51_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP9971666.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8587..8862
                     /locus_tag="IWM51_002272"
     CDS             8587..8862
                     /locus_tag="IWM51_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP9971667.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9300..10250
                     /gene="yidC"
                     /locus_tag="IWM51_002273"
     CDS             9300..10250
                     /gene="yidC"
                     /locus_tag="IWM51_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9971668.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10429..10629)
                     /locus_tag="IWM51_002274"
     CDS             complement(10429..10629)
                     /locus_tag="IWM51_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971669.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
     gene            11434..>11720
                     /locus_tag="IWM51_002275"
     CDS             11434..>11720
                     /locus_tag="IWM51_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9971670.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGR"
BASE COUNT         3553 a         2449 c         2073 g         3645 t
ORIGIN      
        1 aaacggtgtt ccagaagtag cttcttcgga aataagccga aatttcacaa aaatttgaga
       61 aataattttt gtcaatctct tcttatttct tgaagctgac cacttctgtc acaacctctt
      121 ccttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaccaaac aaaaaatatg
      181 aagagctatt tttgcttgct gttcttatta cttgatgcag aacagctttt tcacaacctc
      241 tcatacacgg tgtttaatca gctgaccttc ggtattagct caaaaaacgg aaaaatatgt
      301 gaaactattt ttccgtttgt ttctctaata ctcaggtcaa aacactgatt tcacaacctc
      361 ttttaataac gaagaatcgc tgtcagactc ttttcatctg gcgcatcttt ttgatccaaa
      421 ataaacactt tcccgccatt aatgatcaca tcatctgcta atgcattcaa cacttgacgc
      481 cgatcatatt ctgtttcagg atcttcaccg aaaccatcag ctaagtttga cgtagcgata
      541 aataaatgtg acacttttcc ttctttcgca gctggaacga tatcaactaa ctgatcgatg
      601 aatttcttat ctaagagttt attgtattct tttgattctt gagctgcgag ttcacttgac
      661 agcttcttcg ccccttcttc gatatctcta aaggatagct gcgcaggaga agcagaaatc
      721 gcaacgtctg cacaataata gggattttta gcaatttttt tgaacatcgt ctgattttct
      781 ggtaatgcaa ataaataaat cggccattgt tcaggatttt cgaaagaatc cttcaagtac
      841 gtatcaacag cttggtaata gttgacccaa tcgatctcta cttcttcatc ttttggattt
      901 acgccatgga aagttactcc atctcgggct ccgcctcctc cattagaaga gaagttcaaa
      961 ccgccacctg tcagttcaga tcccaatgca gtaacgacat caaccggtgc cccttctggt
     1021 agagatactt cttttacttg tttgttgctg acctcataca atttcatgga gtcgcggttc
     1081 aatcccaata aataataaga atagttgaac tgcccatttt tgataatagc cagtaaataa
     1141 ggaagatcac ttacatagta ttgatcgtct accgggatac tcaaacgatg gatataggtc
     1201 tggtcagcag ttataatgac cgctacactc gtagttgcac tacgccaaaa cgaaggatct
     1261 cctaacaaat tttcaatttt ttcttggaac ggggaccagt catgttcgga atattttttc
     1321 tcaaaacgtt tctttgcttc tttcgcaaaa tttttgaatt tgatctgatc tttttccaca
     1381 tcttgatggg cgacatgcgt attcagcatg atcgtgacga atggtccttt agcgccttct
     1441 gaagtcaatt gtgtcaataa ttctttatcc ttttttgcca taaacgtacc cctcctaaaa
     1501 aaatcattcc tttgtttcta tctaaagtct accacaacac tatttttcca tacaataaaa
     1561 cgtatctaaa aaaataaaac ggtttctacg gttttaaatt tagctatttt tttaagttat
     1621 cttcataatt tcctcaatta tttagcccca gtcttcaact attcttcatg aaatgctgat
     1681 aaactaagaa aaagaataaa agaggtggat cttatgcatt tgattatgat tttcagttta
     1741 ttaagtatta tcagtttatt tgctttcagc gcttcccgct tattagatgc ggaaacacag
     1801 cctattcctg taaaagtacg taaaaacgac cgctaaactt ccataacgcg cgttcttatc
     1861 ctaaagtttt ttcagaaaca ttgaaactct ctcccctctc tgataaaatt ttaatttgta
     1921 gcgaaacaaa ttaattacaa ggagatcgga tatgtccata aaaaaaacac gtctagtcct
     1981 attattggct agtctcattc tttgccttgc ttcttttcta ccagctgttg ctgtgcaagc
     2041 tgaagatacg ttcaaagtaa atgcaaaagc agcttttgct gtagatgcgc aaagcggtaa
     2101 gattctttat gatcaagatg gagaaaaaac catggggatc gcttcaatca caaaaatcat
     2161 cggtctttac atcgtccttg atcaagtaaa agaaggaaaa ctgtcatggg gtgataaagt
     2221 ttcgatttca gattatgcag aaaatttgag tatcacccca gatttatcga atgttccttt
     2281 gcataaagaa aacacctaca ctgtcaaaga attattcgac tctgcaatca tccagtcagc
     2341 caatgcttca atggtggcct tggcagaaaa aatctctggc agcgaagcta aattcactga
     2401 acgaatgaaa gaacaattga aagattgggg aatcaaagat gcaacaatcg ttaatgcatc
     2461 aggattgaac aactcttatc ttggcgaaaa ccgaccagaa ggcactggag aaaacgacga
     2521 aaatcaaatg tctgctcaag atgtggccat cgttgcccgc catttgatct tggatttccc
     2581 agaaatactg gatgtatcaa gtactacgac ccaaatgttt ggcgaaaata cgcaatcacc
     2641 agtggaaatg gtcaactgga actggatgct cccaggattc gttaattaca aggaaggcgt
     2701 ggacggatta aaaactggta cgactgagct tgcaggtgct tgtttcgttg gtacgatcac
     2761 taaaaacgat caacggatca tcactgtcgt attgaatgcg gacggccatg cggaaaaccc
     2821 gagtgcccgc ttcaatgaaa cagaccgttt gatggactac tgctatgaca attggtcaga
     2881 aaaagagctc ggaaaagcta acgcaagtat tcctagtcta aaaaccattg atgtaaaaga
     2941 cggaaaagaa tcaacagttc ctgttgtgct taaaagccct gtaaaagtat gggtacgaaa
     3001 cgatatggat actggcaaat taacgatcac gccaacactt gacaagaagc aagtcactga
     3061 caatgagatc caagcaccta tggataaagg tacaaaaatc ggtaccgcta cgatcacctt
     3121 ggcagacgat aaattaggct atttagaaga caacaatgca ccatcaacag agatcattac
     3181 ggataaaaca gtggaaaaag cgaatatatt cgtgattggc tggcgacgcg tcgcagaatt
     3241 tttcggcaac ttgttttaat tgaacaataa gaaaaacatc tttccatcgg aaagatgttt
     3301 ttttgctttt tctagtttaa ataacagctt ttttcatcta ttttcgttat actgtataag
     3361 aaaaaggagt tgaatttatg aatctaacca gagatctgat ctttttagtt ctctatctta
     3421 ttggggtatt ctctatcgtt tattggcgaa aacagaagaa ggatccttcc ttgttctttt
     3481 acggcagtac gtggacattt acggtaatct taacttattt tgtcgtgttg gaaatttttt
     3541 attcgacctt ttattttttg attccattgt tgttctttag tatctttgct ttttcttatt
     3601 tcacagaaaa acgaaggcta ctgaatggtc ttttattcaa tgttttactc atcagtttcg
     3661 gcatctactt gtttgtcttg ctgtatgaaa cgcaaaatat ttttcttggc ggactgatcg
     3721 ccttgatcac gattccattg ctacttgtcc ttttatttgg tatttatgga ttgattgttt
     3781 ttcttttttg gaacggggtc acagttttac gtagagaatc gcattcatta gctaatcttt
     3841 taacgttgat actagccatt tttcttacac tgtttcttgt ttttgacttt ttcttgttaa
     3901 aatatttgcc gcaatggata aatgctttgt ttttctgcgt tcccctgatc ttgatctatc
     3961 tatttatcgt attctacaac tttttgactg tctcattctt gtatcagttc aaccggcctc
     4021 gatacaatca agacttcatc gttgtgttag gcgctggatt gatcaatgga gaaacagttt
     4081 ctcctcttct agccaagcga atcaataagg caattgcttt ttatcgggct caaagccgtg
     4141 ctacattaaa tccgcccatc ttattgatgt ctggcgggca aggagcagac gaaaaagtac
     4201 cagaggccat tgcgatgaaa caatatgcca tggaacaagg aatccctgaa agagacattc
     4261 ttgtcgaaac caattcgaca accactttgg aaaacatgct ctattcaaag gaaatcatgg
     4321 atcagcaaat gaaagggcca taccgcgcga tctttagttc caacaattac cacattttta
     4381 gagccggtct ttatgcgcgg caagccaaat tgaaagcaaa tggtattggt gcaaaaacag
     4441 ctttctacta cttgcctaat gcgtttttaa gagaatacct cgccatcttg atgatgaaca
     4501 aaaaaagaca tatgatcatt tgcggattgc ttttcgcagg ctccgcattc ttatcgctga
     4561 ttactttact tttctaaaaa aacaaggtgt gacaaaacat atgtcacacc ttgttttttt
     4621 ctttcattct cttaactatt ggcaaaaaga gtatcccttt tttgaccgct acttgctgag
     4681 cagaataaat accgctgttg cctgaacaca taaagctgat caagcagatc ataaagaagt
     4741 atactgccgc ttgtgaaccg aacaattcga tccccatcac aaagcaggca atcggtgtat
     4801 ttgtcgcacc agaaaatact ccgataaagc ccaatcctgc aagaaaagga acagaaacat
     4861 ggagcaaagg cgctagtgct gcaccaagcg ttgcaccaat ttcaaacaat ggtgtcactt
     4921 ctcccccttg atatcccgcg cctaaagaca aaactgtgaa gaataacttc ccaataaaat
     4981 catagacatg tgcctgtcca ttaaatgatt cttgaagtaa cggaagactc aatccgagat
     5041 aacgttgtgt ttgcaaaatc aatgctgctg ccacaactac agctccacct atcgtatttc
     5101 gacaaacagg atgaggaatc cattgtgcat atcgcgcttt taaaaagacg atcgaacggc
     5161 tgaataccca accaatcaaa ccaaaagcaa tcccagcaac aaaaactttg aagaacagca
     5221 acatactcca ttcaggaatc acacccattt ggtaatgtag atgagaaaca ccatagcttt
     5281 ctgtcatgaa atttgcaaac agtgcagcaa agaagctagg gaagatcgct tctgttcgga
     5341 ctttcccgat tgccaacact tctaatccaa ataatgttcc agctaaaggt gtgccaaata
     5401 cagaactaaa acccgcactg attccactga taatcaagat ttcccgttct gatttatcca
     5461 atcggaacaa acgtgccgta ttatctgcta acgctccccc catttggact gctgttcctt
     5521 cacggccaac agatccccca aataaatggg tagttatggt tccaaacagt gtaagtggaa
     5581 taagccgcaa cgggattttt tcttctccac catttccttg atcgatcact aagttgtttc
     5641 cacgactaga aagtccgccg tgatacgcat agagataagc aaaaagcgca ccactaactg
     5701 gtagtagaca aaggagccaa ggatgtgata gtcggatatc tgtcacaact gtcaggctat
     5761 ttaagaaaaa agcagacaag ctccccatga atataccgag aatcgctgtg atcaccagcc
     5821 acttcagcat atacactaaa atgcgtactg gactcggtaa ttgtttttcc ataaaaaatt
     5881 cctcctcaat aaaaaaacgc ctactaaaga aaggggctgt ccccagactt tagtaggcgt
     5941 cattagctat ctttcgttag catttaaagg cgaacaccat cacctattca cttccattaa
     6001 aagatagcat atttttctgt aaaaaacaat aaaattatcg ttctttcttt acacgttcag
     6061 caaattcata gaaaggagcc aatttcgtta actgttgatt acattggtca ctatcatgac
     6121 aaatataatt cccctttttc gtatacgtgc catcagaacc tgctttcgtt gtagaaagaa
     6181 aaagcgaaac attcgatgtt ttctgacaga ttgcacagat tcccttattt atcgttggtg
     6241 ataaagtacc gctaatccct tttaattttt catcttcgta atagaccatg aattttttct
     6301 gcgtacctgg atcattccag ccgatgtacg tatgttcttg caagtcgatt tcattccaag
     6361 ctggttgttt gagttttttc acttttcgga ataatttctc gatttgtggt ttgctaggct
     6421 gcataaacgg ctgaacgatt tgcttcagct cctccaatgc tctttctgct tgcgcttttg
     6481 tcagttttac gtctaaaact gtcgttaaaa atgtatcgat tctggcagtt cgtgatctaa
     6541 tatgtctcga atcttttcag ctgctagtgc ctgaactgtt tgtaccgttt ttggatcatt
     6601 gacagatgag tacgcattta ataactgttc tacttctctt attataaaaa aatattggta
     6661 tggttttatt gtttgttcca tgattttaaa gtcctttctt ctaaccaaag tgcatacttt
     6721 gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga agatcccgga ctcttttaaa
     6781 taagatacgt tttgcatctt atcatcaccc cctttaatgc ttattattct atgtacatga
     6841 ttcattcgat aaataaaagt aaaccgtatc ttgaacagat agtcaactgc aaacagaaaa
     6901 aaggactgtg acaaaagtcg tgtcaaagtc ccttgtttcg aatgaatggt gttcaaaagc
     6961 tggcatctgc ggtaataagc cgaaacttca caaaaatttg agaagcaatt ttcgtaaatt
     7021 ccttcttatt tcttgaagct gaccacttct gtcacaacct caatatggtc tattattggt
     7081 tttatctatt aaatcgagga ttctttccca aagtaacgtt gccatctctt acgcatactt
     7141 gctgacatag gctgagcggc ttctttgact gcgcagtatt tgtctacgaa tgttacgata
     7201 tatccttctt tgtacttagg cggtgcaatc gttgcgcccc acatgtgctt caaaatgata
     7261 tctcgctcaa gatccgacaa ttcggtgatc ttttctgcat tctttacagc aattcttgga
     7321 tggatatacg catgtgtgcc ttcatcaaat ttcgtcgtac gccaatcata gtagaacaga
     7381 tcatgcaaaa gtccagcacg cgctgttgct ttagcattgc caccccattt ttttgcgagc
     7441 agataactat agtaagaaac actgatggaa tgctccaaac gagtggaatg aacatgctgt
     7501 gtgtaattcg ctaatttttt cactgcttct gtttctaaaa gatcttctac ataagacata
     7561 tattcttcgt cttcacgcca tttttctgtc ataattatcg atctgccacc tttttgaatt
     7621 tcattttctt ctgaaattga tctaagtata ccaccagtgt tcggattatt ctagtcgcct
     7681 tggataaaat agccaaagct taatagttcc ttaatcataa agaaaaatga taaagaacaa
     7741 tccctgccgc aaccccaaca ttcaacgatt ccgcatttcc tttcatcggg atatatacgt
     7801 tctgatcagt caaagacaag atttcctgac ttaccccctg accttcgttc cccatgatta
     7861 ttgcgaagtt atctgatttt tctactttgt ctaatgcgtg tgcttcttca tttaattctg
     7921 tgccatacac tgtaaaccct ttgtctttca ttaaaggaat cacttctgtc aaatcttttt
     7981 gcaacacagg caaatgataa ttgcttccct gcatgctgcg cagcaccttt gtactataaa
     8041 tatctgccgt tcctttgcct aataaaacac cagccatatc aaatgcatct gccgtacgga
     8101 tcatcgtccc tacatttcca ggatcttgta cattatctaa caacagccat cctcctgaaa
     8161 aatctggcgt gccttcagcc gttaacgcca tcactgctaa gattccttgt ggtgtaggta
     8221 gatccgatac agcatccatg acctcatccg aaactagaaa agtagaaatc tctttgtttg
     8281 cggccagcca gtctccccat tctttcatgc ctcgctgtgt cacaaaaagt tcttttattt
     8341 ctgcatcaga cgacgcagct tcttcaatca gatggaaccc ttccaaaaga tattcttcct
     8401 tttcttcccg atatttcttt ttatgtagtt tttttaattc tttgattctt ggattttttg
     8461 ctgattggat ctctttcaca ttcctcacct ccccatgatt atatcatgga ataggtgctt
     8521 gagcttgttt catcctaaaa aaatcggtat cattaattta taaaatagga aagaaaggtg
     8581 acgaacatgc gcaaagttaa aatgaacgtc caaggacgcg tccaaggcgt aggatttcgc
     8641 tacatgacca agatggtcgc tgatcaacta ggcgtgactg gaacggtaaa aaacgaagat
     8701 gacggctcag tcacgatcga agcaatcggc aatgacgaca tcatccagaa atttattgaa
     8761 gaagtcaaga aatctccgag cccaagtgga cgagtgcagt atgtggatgt ccaagaagac
     8821 ccgatgattg aggaacgaaa gaaatttgat gtggtgggat aaggttgtga aaaagctggt
     8881 ctgcatcaag cattaaggac aggttgtgaa aaatagctct ccatattttt caacaggctg
     8941 gacttaatgc cgtagatgca agcttttgaa caccgtcgat aaggttattc ttgaggcgct
     9001 ttgcctcaaa aattaaggac agcagtgaaa aagctgatct atcacttgca gtgagttcat
     9061 ttgactccgc tttttcgggc agaaaaaaag tttttagttt attaaagtaa gaaagaaaca
     9121 cttggaatca ttctactttt tcctagtatt tctttcttga ttttttgtct ttttttcaaa
     9181 atttttcgct acgtaaaaga acgaatattt atatgaaatt tctatgaagt tgattgaatt
     9241 ccatgcgctt ttttagtata gttaagtttg tgtaaaaatt atagaagagg aagtattcta
     9301 tgaataaatg gaaaaactgg ttattgggat ccggacttgt cagtatcctg ctctttttat
     9361 ctggctgtgt gcggatggat tcaaacggaa accctgacac ttcgggaatc gtttatcgag
     9421 tactcgtcca tcctatgggc caagcgatta catatctagt tgaaaatttc aattggtctt
     9481 atggctgggc agttattgca atgactgtga tcgtccgtat catcatcttg ccactaggta
     9541 ttagtcaatc gaaaaaaaca atgatccaat ctgaaaaaat gcaagcgtta aaaccacaag
     9601 ttgaagcagc ccaacaaaaa ttgaaagctg caacaacacg tgaagaacaa atggcagcac
     9661 aagcagaaat gcagcaagta taccgcgaaa acggattgag tatgaccggc ggaatcggct
     9721 gtctgcctct attgatccaa atgccgatct tctctgcctt gtactttacg gcacgttata
     9781 cagaaggtat ccgggaatca tctttctacg gcatcgattt aggctcacca agtatggtac
     9841 ttgttgccat tgcaggggtc gcttatctgc ttcaaggata catctcaacg atcggtattc
     9901 ctgaagaaca gaaaaagacg atgcgtacga tgctgatcgt ctctcctgct atgatcgtct
     9961 tcatgtcgat cagtgcgcca gccggtgtta cactttactg ggtagtcggt ggtgtgttca
    10021 gctgtctgca aacattcatc acaaacgtta tgatgaaacc acgtttgaaa gcacaagtgg
    10081 cagaagagtt gaaacaaaac ccaccaaaac aagtcgtcac tccaagaatt gatgtgacag
    10141 aatcagctaa accagctgac agcaaacaag caaccaaaca aatcaagcaa cccaaaaaca
    10201 gtacaaacaa taaaggccgc aacgcaggaa agcagcagaa gcggaagtag atagtaaata
    10261 agagtgtaat aaaaattcat taacagtttt agcaattaaa aatatcgacc aagatttttg
    10321 aaaaagaaat ctggtcgata tttttattta agtgaatgtt taatttgaat catcgattga
    10381 tcgctatcta attgcagcag catcttttta tgaagaaaca actttttctt atctatcaac
    10441 gtagacaggt gttgctgttt cttctaatag tgtacttaat tccttaatat tttgttttcc
    10501 ttcttctaac atccaactac tcgctttttc ttcgccttcg gtaacaaaag gcgccactcc
    10561 cttcgcccat gttgctcgcc cacataagac accattctcc ataaaggaca acgcggagat
    10621 accacccatc taaataacat ccataaacct ttctagtaga agaatagtga tagtatccat
    10681 ccctttttct actgggaata caattacacg attcaaagct tgatacacaa catttccctt
    10741 cttataaacc gctattgtaa ccgaattgtt ttgaatcacc tttttgggat agaaactggc
    10801 tcctttccgc aacttcttaa atgttggttt cttttatact ttctttgtag caactacaaa
    10861 tttcttcaaa tctctaaatg tttgcataac aaccgccttt ctgactttta ctcaggatgg
    10921 tatttaaaaa tcagcaaaat aaggcttctc tccatctttt ttatttccta gctgctttaa
    10981 agacactctt gctgtttttt aatctctatc aagggggctc tgaatctatt ttgatgaact
    11041 tatcttatta atttctcaaa tgagctgtca gacacaactg tattttttca atctcatacc
    11101 ggacaactat gccagcttaa aaaaatttcc tctggcaaaa cttctttatt gaaaaatgac
    11161 aatgaaaaaa gaccattctt tttgttgaat gctcattgtg taaacatatt tcctgcgact
    11221 gctcttcgaa ccattctcag tgcaatccct aatttatcta ttaaatcttc ttttagttcc
    11281 tcatctttca tattcaaaat ttcctttgta tgttacacaa gtttgttctc taaataacgc
    11341 gaattgtaaa attaagttag aaaaataaaa aggcatttat ggtacactca aattgtattt
    11401 ccgacgaaag aaaacaaagg agtgtaacca taaatgacct acaaacatct taccatagac
    11461 gaactgacaa tgatagaatc atattatctt caacataata agccggttga aatcgctaac
    11521 cgaatgggac gtgctataca aactatttat aatgtagtca ataagttcaa gcaaggtaag
    11581 actgctcttg attattggca ccagtataaa gaaaataaga aaaaatgtgg tagaaaagtc
    11641 attcaattac ctgctcatga agtagattac attaaagaga aagtcactct tggttggacg
    11701 cctgacgtca ttatcgggcg
//
LOCUS       DACDYJ010000078        11623 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDYJ010000078 DACDYJ010000000
VERSION     DACDYJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.079"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(110..634)
                     /locus_tag="IWM51_002276"
     CDS             complement(110..634)
                     /locus_tag="IWM51_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9971671.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(627..1100)
                     /gene="tsaE"
                     /locus_tag="IWM51_002277"
     CDS             complement(627..1100)
                     /gene="tsaE"
                     /locus_tag="IWM51_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP9971672.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1223..2203)
                     /gene="pta"
                     /locus_tag="IWM51_002278"
     CDS             complement(1223..2203)
                     /gene="pta"
                     /locus_tag="IWM51_002278"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP9971673.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2356..3036)
                     /locus_tag="IWM51_002279"
     CDS             complement(2356..3036)
                     /locus_tag="IWM51_002279"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9971674.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3162..4031
                     /locus_tag="IWM51_002280"
     CDS             3162..4031
                     /locus_tag="IWM51_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9971675.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4057..4572
                     /locus_tag="IWM51_002281"
     CDS             4057..4572
                     /locus_tag="IWM51_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971676.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4619..5182)
                     /locus_tag="IWM51_002282"
     CDS             complement(4619..5182)
                     /locus_tag="IWM51_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9971677.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5237..5416)
                     /locus_tag="IWM51_002283"
     CDS             complement(5237..5416)
                     /locus_tag="IWM51_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971678.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5493..6002)
                     /locus_tag="IWM51_002284"
     CDS             complement(5493..6002)
                     /locus_tag="IWM51_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971679.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6098..6478)
                     /locus_tag="IWM51_002285"
     CDS             complement(6098..6478)
                     /locus_tag="IWM51_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9971680.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6807..7556
                     /locus_tag="IWM51_002286"
     CDS             6807..7556
                     /locus_tag="IWM51_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP9971681.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7740..9017)
                     /locus_tag="IWM51_002287"
     CDS             complement(7740..9017)
                     /locus_tag="IWM51_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971682.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9096..9740)
                     /gene="pcp"
                     /locus_tag="IWM51_002288"
     CDS             complement(9096..9740)
                     /gene="pcp"
                     /locus_tag="IWM51_002288"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP9971683.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9939..10883)
                     /locus_tag="IWM51_002289"
     CDS             complement(9939..10883)
                     /locus_tag="IWM51_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP9971684.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10885..11571)
                     /locus_tag="IWM51_002290"
     CDS             complement(10885..11571)
                     /locus_tag="IWM51_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP9971685.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3312 a         2466 c         2047 g         3798 t
ORIGIN      
        1 ccgtttattc aaaaaaaatg gactgcgaca cgacttttgt cacagtccct tcgtgtgttt
       61 ttctgccttc tcaaaaggat gacagtttct cactcttttc ctatttcttc tattcaatca
      121 aataactcat tttatataca tctaaaaatt cacctagatc tgttcttgcc cctctaggca
      181 tgaccgcttc gatttggaag cccatttttc ggtaaaggtg tacggctctt tcattacgaa
      241 cttgaacgtc aagctccaat cgaaaaagta cacccatttc cttcgcccaa ctgatgattt
      301 cttctagcat aagagtgcct agtcccattc cccaatattc ctgcagaata ctgataccga
      361 cttctcccac gtgagacaaa cgaaattgac tgtctgcctt gacagatgct gttccaataa
      421 tcgtcccttc atagattgca agcagcaata aattattatt gctttcgtat atataatcca
      481 actcttcttc taaggtttct ggtggtaaca ggagttctgc ttcatccaaa actaaaaatt
      541 ctgtctcctt attaacttgt cccatcatca aaagaatgtc ttttgcatct cctggcactg
      601 cttcccgcaa gatgatccca acttcttcac tcatgatttt cagcccattt ttctagaatt
      661 cgctgtttga aggtttcact ttgctccccg tatgagatta gtttcacata ccgcttttct
      721 aaatctgtat cacttttttc taaaatcagt tccaaataat cctccggcag tgcttcttct
      781 aatagatttc cccattcaac aacggaaagc ccatcacctt caaaatattc atctaaccct
      841 aaatctgctc cgctagctgc tacccgataa atatccatat gatacaacgg aatccgacct
      901 tgactatatt ctctaataat cgtgtatgtc ggacttttga tcatttgcgt tatccctaat
      961 ccttgggcaa ttcctttcgt caacgttgtt ttacccgcac ctagatcacc agtcaatatc
     1021 agattgtcac ctggcattgc ggaatgacca attgcctctg cgaattgttc tgtcattgtt
     1081 aatcctgata gttcgatcat ttttcatctt ctcccgctct tctttctttt tgccagtata
     1141 tcgcgtaatg aagttttttt aaagaaaaaa tgaactagtc aagaacagca gctgctgttt
     1201 ttggctagtt cgcgtgtatt tgttaatcaa tcaatgtttg tgctgctgtg ataatcgata
     1261 atttatatac atcttcttca tttgctccac gagacaaatc agaaaccggt ttgttcaagc
     1321 cttgtagaat tggtccgatt gcttcaaagt taccgaaacg ctgagcaatt ttatagccga
     1381 tatttcctga ctgcaattct ggaaatacaa agactgttgc ttgaccagct acttctgaat
     1441 ctggcgcttt cagttgtgca actgaaggaa cataagaagc atcaaattgc aattcgccgt
     1501 cgatcatgta ttgtggcgct agttttttag cgattttagt tgcttcaact actttgtcta
     1561 cttctggcga tttcgctgag ccttttgtcg agaagctcat cattgctact tttggttcga
     1621 tatcgaacaa ttctgctgtt tttgcagaat ctacggcgat ttcagccaat tcttgcgcag
     1681 ttggattcac gttgatcgca caatccgaaa agacatattt ttcttgatct cttccacgta
     1741 ccatgatcat cgctccgctt gtccgactga tgccaggctt tgttttgatg atctgaagtg
     1801 ctgggcgaac agtgtcgcca gtcgaatgga tcgctccaga gaccattccg tctgcaatcc
     1861 ccatatacgt aagcatcgta ccaaagtaat tcacatcttt taaaatcttt tctgcttgtt
     1921 ctttggatgc ctttccattt ctacgttcaa caaaagtatc caccatttca tcccacttat
     1981 cgtaattatc agggctgatg atcgtaaagc tagatgtttt gattcctctg gcatgcgctg
     2041 catttcttat ttcttcttcg ttccctacta aaatgggttc cacaagttct tctgctttca
     2101 accgggcagc tgcgcctaaa atacgagcat cagtagcttc cgggaaaacg attttgatcc
     2161 cacggcgaac aattttaaat cttaaactat caaacaattc cacaagatga cctccagacg
     2221 tttttatatg tattatcata cactattcca gaaaaaaaga acagtttatt ttatctgtat
     2281 cagtcaaaaa tgctggaaaa attaagtttt tcacatacaa agcgaactgc actataacag
     2341 gaaagcgctg ttttattaca ctgtttcagg cagttgccaa tcaatcggtt cttctcctaa
     2401 cgcaaggagc gcatcattcg ttttcgagaa gggtttggaa ccaaaaaagc cacgacttgc
     2461 agataacgga ctgggatgtg gagatgttat gatgatatgg cgactcttat cgatcatttt
     2521 gattttttct tgtgccggtt ttccccacaa gataaacacg attggttttt cccgttcatt
     2581 taatttttca atgatcgtgt ctgttaatct ttcccaacct ttcccgcgat gagaatacgc
     2641 ttgcccttcc cgaacggtaa gaacggtgtt gagtaaaagg actccctgtt tagcccatga
     2701 cactagattt ccatgttgaa caggtgcgat tccaagatca tcataaagct ctttatagat
     2761 atttcttaac gatggcggga tctttacgcc aggttgcaca gaaaaagata agccatgagc
     2821 ttgattttct ccatgataag gatcttgtcc taaaatcacc actttaactt cttcataagg
     2881 agtcaattcc aatgcttcat agatatgata catatctgga tggatctttt gggacgcata
     2941 ttcttttttt aaaaattctc ttagatgcag ataataatct tttgaaaatt ctgaggacaa
     3001 aacttcttgc caactgttat gaatgatttc tttcaaaaac tccacctcct aataaaaaga
     3061 ataactcaca ttttcttcaa tcacaaggga caatcttcac cctcagcact tgattaatat
     3121 ggtaaactag gaacaagaag cagaaaggaa cgataatcat tatgatcaaa ttaattgcat
     3181 cagatatgga cggcacatta ttagatgccc atatgagtgt ttctcctgaa aatgccgagg
     3241 cgatacgttt tgcaaatgat gccggtgttg agttcatggt cgctactggt cgaaactatc
     3301 aagaagctcg cgcagcttta gatgaggtag gaatcgattg tgcgatgatc actttgaacg
     3361 gggcacaggt atttgataaa gaaggaaatt cattgtttac cgtgtcgatt ccaaataatc
     3421 aagcaatctc tgttttagat attctagatg ctaacggtat ttactatgaa gtagcaacca
     3481 acgatggtct ttattctgaa aatcaagcga aacgaattga aagttttgct tccatggtgg
     3541 ctacccattt acctcacctt acgtataaaa tggcgattgc catggcctca gctaagctgg
     3601 agttattgca catcacatac gtggacagta ttcgtgctat tttggaagaa aagaatctgg
     3661 aagttttaaa aattatttgt tttcatacag aagggccagc tgtattaggt ccagttggaa
     3721 agcagatttc tcttttaggc gatctagccg ttacttcctc tggtcaaaac aatatcgaag
     3781 tgaaccataa aaacgctcaa aaaggaattg ctgttgccca tgtcgctcac gagcgtggga
     3841 tttctttaga agaagtcatg acgattggcg ataacttcaa tgatgtcagc atgctgcaag
     3901 tagcaggagt cagtttcgca atgggaaatg cagaaatcga agtaaaagat tatgccaaat
     3961 atacaacgga cacaaacctt gaatctggcg taggaaaagc catattgcgc gcgattgcag
     4021 aagacttgta gttttatcta gaaaagaggg atatagatgt cagaattttt cattcaggaa
     4081 aagcagcttg gacgtgcaac tcggacaatt gtcaaaaacg aaaaaggaca ttccctgttt
     4141 ttgatggttg gtcgctgggg aacaaagggc gatgcattgt ctctttatgc catgaatggc
     4201 gatctcgttg ctcacatcaa gcagataagc ctgacattcg gtacgcgttt tgagctgtat
     4261 aaagagttcg aaaaggtggg aaccatgcag aaaatattca attggcccgg tgacttttat
     4321 tatattcgta gacttcactg gacagtccaa ggtgatatct ataatcatca atatcaaatc
     4381 catcatttca atcagcaaat catgaaaatg gataaagcaa cacttttttc aggagattac
     4441 tatgtattgg atgtctcaaa tgatgaagat gctccagttt gtatttgtat tgctgcggtt
     4501 ttagattact ggctctaccg taaagacaaa agtcgcgata agcctgtttc ttaccgactt
     4561 aacttaagtt aaaaagatgt acctgaaatg cggaaatacg catttcaggt acatcttttt
     4621 agatattctt ttgcatatga tcataaagat gaccactgat catccgctga ccgacaactt
     4681 tgaagtcatg gcgttcgtat aattttttag cctttggatt tttttcatcc acacttagac
     4741 cgatgacgga gcgattagct ttcttcgcca atttaggtaa tgcttctaat aatcgcgaac
     4801 cgatgccttg tcctctaaaa tcttctgata cactgataga gtccaaatac cattcgttgg
     4861 gaaacgtttc tttatcaatg aacaaacgtg tagacgcatc tagctgatat ttctctaaga
     4921 taggtaccag tggctgatcg atgattgatt cttcttcatc tgtataaccg taagcaactc
     4981 ctgcaacttt accgtctatt tcttctacga tacctcgtga ataactgtaa cgataatttg
     5041 gatcagtgat tgcttcttct aaaatctcta aagtcttttg ttcccctgtc tgcttcaaaa
     5101 atggcagttc catatctttt agaatcacta agatcaatgg ggcgatttct ggagcatctt
     5161 cttttctcgc aaaacgaatc atagtttcct ctccttttct ccattctaac atgcagaaaa
     5221 agaaatcgca aaatcgttat tttaaaaaat cttcaatctt ttcagtaatg aaataaccat
     5281 gagtaacgat tcttgccgct tcagttcggc agtaagggca gacgactggc atttctttat
     5341 cattatgttc gactacatta ttgacttctt cgtatgcaaa tttttttccg caattagggc
     5401 atttcatttt ttccatgtta tctcaacttc ctttcatctt tactctctta gcttactctt
     5461 atattgtaaa aaagagaagc aatatccttc ttctatgcag atatccatac ctgacggtcc
     5521 tgttctccat ctattaatcg gactttataa taaggacgtt catctttgac tagaagtgtc
     5581 cactctttca cttttgcttt tttcccgtag tagctgactg cttccttcat cgctgcttgc
     5641 ggttgtttga actcttccgt tttttctata tcaaaaagat gttctgcggc cttttccgtg
     5701 tcatttcgta cggttgtttt cccagtggat ggattgactg ctatgacttg tccttcacaa
     5761 aaaaactggt attcatatgt tgcattcgta ggattctcca aatctttttc aactgcaaaa
     5821 tgaatagcag atatgttatg ttcttttgtt tcgtttttta cgattgctgc tgcttttttc
     5881 aatgagatcc gatattcctg gtcactaata tcatccatgt gaaaagcggc attgagcagt
     5941 cccgtcatac cggtaatccc tattacgatg agggcagcaa caccaaacat catttttttc
     6001 attcttcatt ctcctgtttc ttttttttaa agaatatcaa gaaatgaacg aatgaaaaat
     6061 cgaatgaaac gaaaaaaaag tgacaatttt gttatcttta ttcgtgcagt tctaatggca
     6121 atccgtctgg atcaaagaaa aacgtcattt tttttcctgt aaaggtatct tctctaatcg
     6181 gttctgattc gatccctaag gactgcaatt ctgcaattgt ttcttcaata tcgtctactt
     6241 tgaaagcaag atgacgcaag ccacaagctt ctggatagtt tggtcgttgt ggcgcattag
     6301 caattccaaa aatctccaat tcagcacttc ctagtttcag atctaattta tagtcatctc
     6361 gttctttgcg gtaattttct ctgattactt caaatcctaa tttattaaca taaaaatcac
     6421 gtgatttctg ataatctgaa acaatgatgg ctatatgatg gatcgttgta aggttcatat
     6481 gagcactcct attcttcgtt taagctcgga tctatattaa tctccagttc cattgtaaat
     6541 gtgtcagatt gaaaaaaata gctgtttttc ctttttataa gaaaaaaggc aaaaaaaaca
     6601 gaaagtaggt cctccaagac actcttcctg ttcccaatat aataaatgaa agtaagaaat
     6661 tatattttca agaaaacaaa cagaaaatta tcataacaca aattatttat aattgaaaga
     6721 ctagtttttt tcaagaaaag catacaatag attgtgttag taaatacaga acacactaca
     6781 tttagtgtct taaggagtcc tcatacatga agtcatattt tcagttttta gctcatcagc
     6841 taaaaggctg gccgcaacaa aattattatc ttttcttttt cagtcttggc tgccaggtca
     6901 tgactcttgt caatagtccc atcacttggc tgtccgttat tacatttatc ggtaccacgc
     6961 ttggtgtatt gtgtatttta tccatcaatg cggcaaaatc agtcaacggt attttaggta
     7021 ttttgtctgc gatttgtttt attatcgttg ggttttcagc aaagaactat ttaagtatcg
     7081 gtgaacagat tgcttatatg atcacattgg atatccccgt attgttaagt aaagaatgga
     7141 atcataatat ggcttcaaaa atccgaaaat tcactggaaa aagctggttt atcgcaatca
     7201 tcagtacttt ccttgtctat cttgtttcag gattcctgat tggtcgttat acagatgatc
     7261 ctcggccgtg ggtagatgcc attgcttttt ctattagctt gactgctggt atcatcagtt
     7321 tcttgcgtta caacaaccaa tattactggt ggttagcttc agggcttgct caaatggtcc
     7381 tttggtatat ttcatttacg catggatctg caagtttggc gatgttcgtg aacagctctg
     7441 tttacttgct gaatgatgtc cttgcattta ccgtttcacc ttggtataac caaaaagaac
     7501 gtgaacgtat gcaaaaaatg gaagaagaat actacaacgc acaacaacac gcataaaaaa
     7561 actgagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg
     7621 gcggaatcag aagcaactcc ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa
     7681 ttttcgtgaa tttcttctta tttcttggag ttaaacgctt ctgtcccagc ctctattttt
     7741 tatagtaatt ccttcagata ctttcttgcc tcatcattga aacgcagtga ttcttctaac
     7801 ttctgataat attccaacgc ttgtttctga gcttcagtaa aacgataatg ttttcctttc
     7861 catttgtcga ttgtctcttg cacatttcca gattggatat ctttcactac tagttgccat
     7921 ttttcttttg agactgtacg gtctttctta tcagcagatt gtatagcttc tcgttcttta
     7981 cgcaatactt ttaaaaaacc atttctgact atccgaggaa tgctgtcgaa gaacccttct
     8041 gtaaaaccat agcgacaata ttgcttccaa ataaatagat agatttttgg ttggatgaat
     8101 cgttggtttt tcgcaaaata ttccatctga gcagtgactt ctggtgttcg ttgacgtttt
     8161 gtctttctca acacggaaat aaatgtttca aaataagcat taggatgtac tggcacttct
     8221 tcatatacga tggattgcca atctttacgt aaaaatgcgc gatagttgat caagaattct
     8281 tctacatcct ttactaattc ttctgcaaag tcgtcaaata gttctttttt tatctgtcgc
     8341 ttttttgact gcccattgat gtgttcactt attttcttga tttcattttc caatgcttga
     8401 taatccgcta tgtaaatcgg catttctact tggcgaaatt ctgcgagccg ctgagctaat
     8461 tttatatccg gtaaatatgg ctgtttcatc cgacgatttt ggtacaagac aaaacagtat
     8521 ttttgccata gtccttcagg aaaatcgata ttcttctgct taagccataa taattcgtct
     8581 aaggccagat caacttttgt cattccctga tggatcgaag ctgccactgt cggagagaca
     8641 tgcaggtgat cggcaaagtg gttgatgcct agttttagga tctttccggt ttttggcgat
     8701 ttaacgatgt attgcacttt taattctcta ccacactcac aatacaattt ttgctggctt
     8761 cgttttgttc gaaaaaacgg attgatcttg aagtcagaaa acacccaatc tgttgcagct
     8821 aaataatgtt cttccataaa acgagagctg attagatatc ggcggtgctt atcgactagt
     8881 tcccgctgtt ttttcgttaa ttttgcatag atctcccaac gttgactgct tgtgtattgt
     8941 ttagaaattt tgatgggata gcttttgctg atttcttttg gaaaccattc ttcaaagacg
     9001 gattgccatt cttccacggc tgtttctttc ctttctaatt acaaaaagct ggctactaac
     9061 ataacgttag caaccagcca gtgtttcttg ctaaattagt gagttgttcc ggcatttccg
     9121 gaaatatctt ctttattttc tactgctgct tcaactgcat aagtcaaaga agaagcaatg
     9181 tcttctaaag acatagaagg cgtacccact ggacgatcga tcacttgatc gggtgagaat
     9241 ggcacgtgga tgaacccacc tcgaattgcc gggaattgtg tttcgatcaa atgaagcaaa
     9301 cgatacatga tatcattgca gacaaatgtt cctgctgtat aagagatgta cgctggtaga
     9361 ccatgatctt gtacattttt tgtcattgct ttgattggca atgaggtaaa gtaagcggct
     9421 ggaccgtttt cttctaaagc cgtatcgaat ggctgatttc cttcattatc agggattctt
     9481 gcttctgcta gattgattgc gacacgttcg aacgagactg ctgaccgccc acctgcttgt
     9541 ccgacacaga tcacgacatc tggttggtgc tgttggatgg cttctttgac tacttgactg
     9601 cttttttcga atacggtcgg tacttccact ttgatgattt cagcaccgct gatttcatct
     9661 ggcatttttt ttactgcttc ataggctgga ttcactttgt ccccaccaaa tggatcaaaa
     9721 cctgttacta gtactttcat ctgttgttcc tccgtccgat tgatttaaca tatggttatc
     9781 ttttatcata ccattttcct tgaacagcgt aaataaagga aacaatagtt taaataacat
     9841 aacagatcaa acttaatata ttcttataaa aaaatagtgt tccactacaa tgttagaaga
     9901 ggttgcgaca attattctgt cacaacccct ctcttctatt atttaaatag aatcatataa
     9961 gcaatttgga aaactagcat aaataacgca ataaacaact ggtttttgat cactccgtat
    10021 ttgtctttca tctccagcat cgctacaggc acgatattga agttagcagc catcggtgtc
    10081 agcagggtcc cacaaaaacc gcaagtcaat gcaaccattc ctattaacgc tggattcgct
    10141 ccatgactaa atacgaatgg tgcaccgatc ccaaccgtca tcacggtgat ggccgcaaat
    10201 gcattcccca tgattaccgt aaatatcacc atgccaagag cgtaaatgat gatgccgatg
    10261 ttcacatttc cttctggaac gatcgtacct actgcattgg aaatcactgt cccaactcca
    10321 gcgcttgtaa ataccgcacc tagagaagct aaaagcattg gcaacatgct taacggccct
    10381 actgtgccca gcatatctgc tgcatcatct aaaaatgtac ttggtcgatt atctcgagaa
    10441 aagaacatca agattagcat ggctgcaaaa acaccgacgc ccacgccgac taaagcacct
    10501 aaattggtaa atagagcaaa caagatcgca aagacaccga tactcaaagc tggaataaaa
    10561 attttcattc ctagtttgtc tgacattttt tccatatatt cttttgatgg aagccggctt
    10621 tctcctttgc tgactttctt agcaattgct ggtatggtca tcgcaaagat caagccaccg
    10681 ttcacgattg ccggaagcca tcttcctcct gcaataacta cccctaagac cgtccagaaa
    10741 aaggctgacc cataacgatg aggattggtt ttatctgtta aatttcttac tccagtgtaa
    10801 atcaatacga gtcccatcac gatataaagg atctccagta atttatcgcc taataaaata
    10861 tcaggatttg taaaaaagtc catcttattg cccctttcct ggataatatt tcttcgctaa
    10921 tttcttttct ttaagataga agtagacact tgccacaact aaagccgtga tagccactgg
    10981 aacttccact aaagcaagct gtcccaatgt tacgtcatat cctagatctt tcagtgttga
    11041 ttggactagc aacgcccctg ctcctccgat gaatagtact tggccaaaga accagcagac
    11101 attttccata gctgaagcca taccttttag ttcttcttca tgcttaggat ggacttctaa
    11161 cccttttgat tcgatggtcc ccaatgccat cggtaaaatg attggacgaa caaatccagc
    11221 aactccacca aaactcacgt taaaagccgc aaagatcatt cgaaagatcc cataaatatc
    11281 tacaatcata ccagcagata ctttcttgaa tcgcttgatc aaggaagccg ctgattcttt
    11341 cagaccattt ctttcaagcg ttcctgttgc caacataatg atgataaaaa tcgccatccc
    11401 gcgattgttt acaaaacttg tacctaaaag ttctaacatt ccttgaatgc caagaccgcc
    11461 agtcaaagct gtaacaacta aagcaaccat tacgatcaat atcgagtcta atttaaatgc
    11521 aaacccgaga ataacaatca agacgcctaa aaggctaaga taattttcca tatattttcc
    11581 tccataaaat gacaatattt ttttaattat aaagcaaaac aca
//
LOCUS       DACDYJ010000079        11596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDYJ010000079 DACDYJ010000000
VERSION     DACDYJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.080"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            62..147
                     /locus_tag="IWM51_002291"
     tRNA            62..147
                     /locus_tag="IWM51_002291"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:96..98,aa:Leu,seq:taa)
     gene            158..231
                     /locus_tag="IWM51_002292"
     tRNA            158..231
                     /locus_tag="IWM51_002292"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:192..194,aa:Arg,seq:acg)
     gene            241..314
                     /locus_tag="IWM51_002293"
     tRNA            241..314
                     /locus_tag="IWM51_002293"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:275..277,aa:Pro,seq:tgg)
     gene            334..406
                     /locus_tag="IWM51_002294"
     tRNA            334..406
                     /locus_tag="IWM51_002294"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:367..369,aa:Ala,seq:tgc)
     gene            434..507
                     /locus_tag="IWM51_002295"
     tRNA            434..507
                     /locus_tag="IWM51_002295"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:468..470,aa:Met,seq:cat)
     gene            521..594
                     /locus_tag="IWM51_002296"
     tRNA            521..594
                     /locus_tag="IWM51_002296"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:555..557,aa:Ile,seq:cat)
     gene            618..707
                     /locus_tag="IWM51_002297"
     tRNA            618..707
                     /locus_tag="IWM51_002297"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:654..656,aa:Ser,seq:tga)
     gene            720..793
                     /locus_tag="IWM51_002298"
     tRNA            720..793
                     /locus_tag="IWM51_002298"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:754..756,aa:Met,seq:cat)
     gene            812..887
                     /locus_tag="IWM51_002299"
     tRNA            812..887
                     /locus_tag="IWM51_002299"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Asp,seq:gtc)
     gene            889..959
                     /locus_tag="IWM51_002300"
     tRNA            889..959
                     /locus_tag="IWM51_002300"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:921..923,aa:Gly,seq:tcc)
     gene            973..1046
                     /locus_tag="IWM51_002301"
     tRNA            973..1046
                     /locus_tag="IWM51_002301"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1007..1009,aa:Ile,seq:gat)
     gene            1071..1158
                     /locus_tag="IWM51_002302"
     tRNA            1071..1158
                     /locus_tag="IWM51_002302"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1105..1107,aa:Ser,seq:gct)
     gene            1180..1251
                     /locus_tag="IWM51_002303"
     tRNA            1180..1251
                     /locus_tag="IWM51_002303"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1213..1215,aa:Glu,seq:ttc)
     gene            1274..1363
                     /locus_tag="IWM51_002304"
     tRNA            1274..1363
                     /locus_tag="IWM51_002304"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1310..1312,aa:Ser,seq:tga)
     gene            1376..1449
                     /locus_tag="IWM51_002305"
     tRNA            1376..1449
                     /locus_tag="IWM51_002305"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1410..1412,aa:Met,seq:cat)
     gene            1468..1540
                     /locus_tag="IWM51_002306"
     tRNA            1468..1540
                     /locus_tag="IWM51_002306"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1501..1503,aa:Asp,seq:gtc)
     gene            1544..1616
                     /locus_tag="IWM51_002307"
     tRNA            1544..1616
                     /locus_tag="IWM51_002307"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1577..1579,aa:Phe,seq:gaa)
     gene            1621..1691
                     /locus_tag="IWM51_002308"
     tRNA            1621..1691
                     /locus_tag="IWM51_002308"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1653..1655,aa:Gly,seq:tcc)
     gene            1704..1777
                     /locus_tag="IWM51_002309"
     tRNA            1704..1777
                     /locus_tag="IWM51_002309"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1738..1740,aa:Ile,seq:gat)
     gene            1789..1860
                     /locus_tag="IWM51_002310"
     tRNA            1789..1860
                     /locus_tag="IWM51_002310"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1822..1824,aa:Glu,seq:ttc)
     gene            2126..3319
                     /locus_tag="IWM51_002311"
     CDS             2126..3319
                     /locus_tag="IWM51_002311"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP9971686.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3449..4921
                     /locus_tag="IWM51_002312"
     CDS             3449..4921
                     /locus_tag="IWM51_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9971687.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5004..5792
                     /locus_tag="IWM51_002313"
     CDS             5004..5792
                     /locus_tag="IWM51_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP9971688.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5859..6527)
                     /locus_tag="IWM51_002314"
     CDS             complement(5859..6527)
                     /locus_tag="IWM51_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9971689.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6642..8354
                     /locus_tag="IWM51_002315"
     CDS             6642..8354
                     /locus_tag="IWM51_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9971690.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8357..10129
                     /locus_tag="IWM51_002316"
     CDS             8357..10129
                     /locus_tag="IWM51_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9971691.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            10255..11594
                     /locus_tag="IWM51_002317"
     CDS             join(10255..10696,10696..11594)
                     /locus_tag="IWM51_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP9971692.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT         3533 a         2024 c         2592 g         3447 t
ORIGIN      
        1 accttgccaa ggtgggggtc gcgggttcga accccgtttt ccgctttgcc aaagcaatcg
       61 cgccggggtg gcggaactgg cagacgcaca ggacttaaaa tcctgcggtg agtgatcacc
      121 gtaccggttc gattccggtc ctcggcattt caaatatgcg cccatagctc aactggatag
      181 agtgtttgac tacggatcaa aaggttaggg gttcgactcc tcttgggcgc gttaattaaa
      241 cgggaagtag ctcagcttgg tagagcactt ggtttgggac caaggggtcg caggttcgaa
      301 tcctgtcttc ccgataatta taaatttaaa cacggggcct tagctcagct gggagagcgc
      361 ctgctttgca cgcaggaggt cagcggttcg atcccgctag gctccatttc atttaaataa
      421 tgaatatctt tttcgcggtg tagctcagct ggctagagcg tccggttcat acccgggagg
      481 tcgggggttc gatcccctcc gccgcgattt ttaagagcct ggacctttag ctcagttggt
      541 tagagcagac ggctcataac cgtccggtcg taggttcgag tcctacaagg tccattgtgg
      601 cgttgtaagt ttcatatgga ggattaccca agtccggctg aagggaacgg tcttgaaaac
      661 cgtcaggcgg gtaaaaccgt gcaagggttc gaatccctta tcctcctttt tcatattatc
      721 gcgggatgga gcagtctggt agctcgtcgg gctcataacc cgaaggtcgt aggttcaaat
      781 cctgctcccg caattaaaaa ctaaaaacac tggttccgtg gtgtaggggt taacatgcct
      841 gcctgtcacg caggagatcg cgggttcgat tcccgtcgga accgccatgc gggtgtagtt
      901 tagtggtaaa accacagcct tccaagctgt tgtcgcgagt tcgattctcg tcacccgctt
      961 ttagtttttt atgggcctat agctcagctg gttagagcgc acgcctgata agcgtgaggt
     1021 cgatggttcg agtccattta ggcccattag tacaaaatct tattggtttt ggggaagtac
     1081 tcaagtggct gaagaggcgc ccctgctaag ggtgtaggtc gggaaaccgg cgcgagggtt
     1141 caaatccctc cttctccgtt tcaatatttt gttttacctg gcccgttggt caagcggtta
     1201 agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc cgtacgggtc attaatttaa
     1261 tatagtttta catggaggat tacccaagtc cggctgaagg gaacggtctt gaaaaccgtc
     1321 aggcgggtaa aaccgtgcaa gggttcgaat cccttatcct cctttttcat attatcgcgg
     1381 gatggagcag tctggtagct cgtcgggctc ataacccgaa ggtcgtaggt tcaaatcctg
     1441 ctcccgcaat taaaaactaa aaacattggt tccgtggtgt aggggttaac atgcctgcct
     1501 gtcacgcagg agatcgcggg ttcgattccc gtcggaaccg tttggctcgg tagctcagtt
     1561 ggtagagcaa tggattgaag ctccatgtgt cggcagttcg attctgtccc gcgccattat
     1621 gcgggtgtag tttagtggta aaaccacagc cttccaagct gttgtcgcga gttcgattct
     1681 cgtcacccgc ttttagtttt tatgggccta tagctcagct ggttagagcg cacgcctgat
     1741 aagcgtgagg tcgatggttc gagtccattt aggcccattt attttattgg cccgttggtc
     1801 aagcggttaa gacaccgccc tttcacggcg gtaacacggg ttcgaatccc gtacgggtca
     1861 tggaagatac tttttgagta tctttttctt tttatctgaa agaatgattt taaaaagctt
     1921 agcagagtta tctttatgga tgattgtgct aagctttttt ggacagttct aatgtaaaat
     1981 atttttatat tttttaagca aaaaaatgaa aaaatacgag cagaaaactg ttttatctgc
     2041 tatacttggt tcgttgcaag ttcccgaaag gatccgcagg acggagatgc cttgtaaccg
     2101 aatttaacta gggggaatca ttataatggt agaaagacac ttatttacat cagaatctgt
     2161 ttctgaagga catccagata aaattgctga ccaaatcagt gatgcaatct tggatgcaat
     2221 tttaaaacaa gatccaacag cacgagtagc gtgtgaaacg tctgtaacaa ctggtcttgt
     2281 tttagtattt ggagaaatat ctacgacggc atatgtagat attcaaaaag tcgtacggga
     2341 aacaataaaa gaaattggtt atacacgtgc aaaattcgga tttgatggag atacagcagc
     2401 tgtattggtt gcgattgatg aacaatctcc tgatattgct caaggagttg atgaagcact
     2461 tgagatccgg gatgaagata aaaaagacgt attagatgag attggtgctg gtgaccaagg
     2521 tttaatgttt gggtttgctg tagatgaaac accagaattg atgcctttac caatcgcttt
     2581 gagtcatcgt ctggtacgac gcttggcaga tttgcgtaaa tcaaatgaat taacgtattt
     2641 acgtccagat gcaaaatctc aagtaacggt tgaatatgat gatcaaggac aaccggaacg
     2701 cgtagataca atcgttattt caacacagca tgatgatgca gtagataatg aaacaattcg
     2761 tcatgatgtc atcgaaaaag tagtgaagga agttattcca gctgaattat tagatgatca
     2821 aacgaaatat tatatcaatc cgactggccg atttgtcatt ggtggtcctc aaggggatgc
     2881 cggattaaca ggaagaaaaa tcattgttga tacgtatggc ggttatgctc gtcatggtgg
     2941 cggtgctttt tctggtaaag atgcgacaaa agttgaccgt tctgccagct atgctgcacg
     3001 ttatattgct aaaaacattg ttgcagcagg gcttgctcgc aaagcagaag tacagctagc
     3061 ttatgcgatt ggtgttgctc aacctgtttc gatctcaatc aatactttcg gtactggaac
     3121 ggttccggaa gaagaactga ttgctgcagt gagagaaaac ttcgatcttc ggccagcagg
     3181 aatcatcgag atgcttgatt tgcgtcgtcc gatttacaaa caaacagctg cttacggtca
     3241 ttttggtcgt acagatgtag atttgccttg ggaacatacg gacaaagtag atgcgttgaa
     3301 agcaagttta gcaaaataag ttatattcag ctggaacgat cgccaagtgg tggttgttcc
     3361 agcttttttt atggcagtta acctcttgaa aaagatagta attatttatc tatttttaga
     3421 tcctgtcata atgaaaggga ggaataagat gaaaaaagaa acaaatgtta ctttggtcac
     3481 agtgagtatt ttcattgcaa cgttcatgac ggctattgaa ggtacaatcg taacaaccgc
     3541 tatgccaacg attgtcggtt cattacatgg aatggaaatc atgaactggg tattttcgat
     3601 ttatttgctg acaaacgcta tgcttactcc gatttacgga aaacttgcgg ataaagtggg
     3661 acggaaacca atctttattt ttgggacatt attgtttatt atcggttcgt tattttgcgg
     3721 tttatctcaa agcatgattg ggttgattgc tgctcgtgcg attcaaggga tgggtgctgg
     3781 ggcaatgatg ccagtagctt tgacgatcat cggcgatctt tattctattg aaaagcgagc
     3841 aaaagtttta ggtttgaata gttcagcctg ggggattgcc agtgtttttg gaccgcttgc
     3901 gggaggattc atcgttgaaa cgatcagctg gcactggatc ttttttatca atgtgccgat
     3961 cggaattcta ttaattattt tgatttggct atatttaaat gaaccaaaaa tcatacatga
     4021 gtctaaacca atggatattt ttggtagttt tagtttgatg gccatgcttg tctcattgct
     4081 gttagggttc caattattag gagacgaagg aatgagtccg cttgtttctg ctttgctgct
     4141 aggtagtatc gtaatgttag gtatttttgt ttttgccgaa aaaagagcta aagatcctgt
     4201 catcatgatg catttattca aaaatccact atttgttgtg gtcaacttgg ctgcagcatt
     4261 ggctagtggt tttttgatgg gaatcgatgt ttatatccct atgtggatgc aaggtgtatt
     4321 agggctgaat gcagcaattg gtgggctggt attagcacca ttatcactag tctggatggt
     4381 cggttctttc cttgcaagca agtggatgac tcaaatgaaa actcattggg tcttgagaat
     4441 tggactacta atcgcagcta ttgggggcgc ttggttgtat cttatgcctt tcagaagtaa
     4501 ctatttatgg ttttttgcta tatcaacggt catcggtata ggcttaggtg tgaccatgac
     4561 tacctcgact gtcagtgcac aaaatagtgt atctcgtgac cagcttggtg tagcaacttc
     4621 ttttaatacg ttagttcgaa caattggtca aacagtcatg gtttctattt ttggtttatt
     4681 gataaatggg gtaactaaac gagaacttgc agcagcaaat ttaacaaatg atgcagatat
     4741 catgaataaa ctggtgaatc cacaaacggc taaatatatt gatgcggata ttttgttacc
     4801 attaagaaaa attctttatg atgggttaca ttccgtttat ctagttggct tgttactcgt
     4861 gatcattgct ttggtcttaa cgcttttcgt tcatgaaaaa agtggtaaaa taaaacagta
     4921 gtttcggtta agatatgtac gtagccgaat cattcaaatt taaagttatt caaaaataac
     4981 ttcttactaa ggaaggcgaa aatatggcat ttattcaagc aaatatttat tcgaatgtgt
     5041 tagaaatgga agtcaatcta aatgtgatac taccgcaaac aacaaaaaag gtgattggca
     5101 ccagttcaga gcaagagaca acggatatac ctgttttata tttacttcat ggaatgggtg
     5161 gcaatcatag tgtatgggaa agacgtacgt cgattgaacg ttatgtagca aactacggat
     5221 tagctgtcat catgccatct actgatcttg gctggtacac agatacagtt tatgacatga
     5281 agtattggac attcattgca gaagagctac ctgagatttg tcatgaatta ttcccgcaat
     5341 taacaagaaa aagagaaaaa acatatgccg ctggattatc gatgggcgga tatggcgcgt
     5401 taaaactagg ccttgcaaaa tcagaaaatt tcgcagcagt aatctctctt tcaggcgctg
     5461 tttctgtagg aaatcgaatg gatgatcttt tgatggttcg taaagcatct ttttgggaag
     5521 ggatctttgg accattggat caaatcgaag gttcaaaaaa tgatccgcaa tacttattga
     5581 aagaactagt aaaaagcgga aaaccagttc caagatttta tatggcttgt ggggaatctg
     5641 attttcttta tcaggcaaat caggaaatgg tatctgctat gcaggaacaa ggcttggcgg
     5701 tcacctttga acaaggacct ggcgatcata actggatctt ctgggaccaa tggattcaac
     5761 gtgcattggc gtggctaatg gaagaaaaat aaaaaaagag tcttaaaaag atagctccta
     5821 tagagatcac aaacatagtg atctctatag gagctttttt actgctcgtt ttctcttctt
     5881 ttattttgtt ggcataagcc ttcaatatag gtttgtgcac tcgttagaaa gagtgctttt
     5941 tcattcaatt ctgcatggga ctgcatactt tctttaaatc cctctaattg attttcaact
     6001 acataattac gcataaatag aactgtttct ttcaaaaatt catttccgtc cataattttt
     6061 tgtcgaagct gctgttcatg ctgctgatcc attaactgtc caatcaataa attgtggaga
     6121 gaggcaatgg cctgatgact ttgggtgact gaaaatcctt cacgaacaag taattggatc
     6181 atttgctcca aatgttctaa tcgactcgga aaagcgggca cagtattgat ttctaattct
     6241 gccccacaag ggaaacgtgt ataaaggtca tagtaatttt ccatatactg cagcaactgt
     6301 tcattccaag gcaaagttgg atctggaagc gctaattctt cttccattgt ttcagccatt
     6361 ttttgcaaga gctgttgttt gtttttgaaa taccaatata aagcaggtgc ttgaacatga
     6421 attctgtttg cgatgtttcg catggaaagc tgcgaaagag cttgtgtttc agccaggata
     6481 tcaaaggcgg cctgaatgat gattgcttga gaaagcttta tttccatttt atcaacctcg
     6541 tgtattgcat tatcttttta aacatggtaa attgttagtc ggtctaattt tacagtgtta
     6601 aattagtcct gtcaatttaa acaaaggggt gtaatcaacg aatgaaatta atcttacgac
     6661 atgcgaaaaa atataaagta gctgttttta tttccttgct gtccgtagca gtcatggttg
     6721 cagcagcatt atggcaacct aagttactac agcaagtatt ggaagctatt attacagaag
     6781 ataatgatga aatgaaaaca attggtattt acctgatcag tctggctttg ctaggattag
     6841 ccgcaggtgt aacgaacacg attttctctg caaaagtagc tcaaggcgta agtgcagata
     6901 tccgagaaga agcatttcga aagatccaga cattttcatt tggaaatatc gaacagttct
     6961 ctgctggaaa tcttgttgtt cgtctaacaa atgatatcac gcaaatccaa aatttagtga
     7021 tgatttcttt gcaatcactt tttcgaattc cattcttatt tattggtgcc ttcattttag
     7081 ctatgatcac gatgccgcaa ttatggtgga tcatcgtagc gcttatcgtt acggtatttc
     7141 tgattacggc actttcattt acaaggatgg gcaagcactt catgatcatt caaaaactga
     7201 ttgatcgtgt gaatagcatt gcaaaagaaa acttactagg gattcgtgta gtgaaatctt
     7261 ttgtacaaga agaaaaccaa ttgaagtcat tcagcaaagt aagtgaggac ttaacaaaac
     7321 acaacatcat agtcggtaca ttattttctg tgatgatccc gtccttcatg ctggctgcca
     7381 atttggctgt ggttggggcg atcttttttg tgagcgatct tgttaaagat gatcctacgt
     7441 taattggtgg aattgcttca ttcatgaact acttgatgca gattatgatg gcaatcatta
     7501 ttggcggaat gatgatgatg atgacttcaa gagcagccgt atcgctgaag cggattgcgg
     7561 aaattttaga tgctgaacca gatcttactt atttagacgt tccagaacaa gagttgacgg
     7621 gttctgtcac ttttgaccat gtttcttttc gttatcctgg tgaggataca gatacgctta
     7681 aagatatcag cttttctatc aaacctggag aaatggttgg tatcgtcggg gcgactggtg
     7741 ccgggaagtc aacactagca caattgatcc ctcgtctatt tgatccgaca gaaggcaaag
     7801 ttgaagtagg tggtgtagat cttcgtcaag taaatgaaaa gagtttaaga caaacggttt
     7861 cctttgtttt gcaaaaagca attttattct caggaacgat tgctcaaaac ttgcgccaag
     7921 ggaaaaagaa tgcaacagaa aaagagatgg aacatgcttc ttctattgct caagcaaaag
     7981 aattcatcga aaagttatct gatcgttacg aagcaccaat agaagaacgt agcagtaact
     8041 tttctggtgg tcaaaagcaa cgtttatcca ttactcgtgg cgttatcggt aatccaaaga
     8101 tcttgatcct agatgacagt acgagtgcac tggatgctcg ctctgaaaag ctggtccgag
     8161 aagcactcga tcgagatttg aagggaacaa cgacgattgt catcgcacaa aaaattgctt
     8221 ctgttgtcaa agcagaccgc atcttagtat tggatgaagg cagattagtc ggcgaaggaa
     8281 cccatgagga attggtagct ggaaaccgtg tctatcagga aatctttgaa actcaaaaag
     8341 gaacggagga gtaagtatga ctgatctaat aaaagcaagt aaatttttct atcactattt
     8401 gaagcggtac aaactctcct ttttctttat ctttatcaca gtgatcattg ctacctatct
     8461 acaagtaaag gcacctcaat acatcgggga agcttttcaa gaattagcta attacgtagg
     8521 aggcttgatg caaggcgtag acgataaaag caaatttgtt tctgtaatat ggaaactctt
     8581 gctgttttat gtactagcta gtgcagccaa ctttatctat agtattttgt tcacacaagt
     8641 agtagggaag tcaacgaacc gaatgcgtat cggtctattc aacaaattgg aaaaattgac
     8701 gatccgtttc tttgattctc atcaagacgg tgaaatttta agtcgtttta ccagtgactt
     8761 agataatatt caaaatagtt tgaaccaagc tttgcttcaa gtcatcacga atgctgtttt
     8821 acttgtcggg atcttgatca tgatgttccg ccaaaatgta gagttagctt gggctacaat
     8881 tgcttccact ccagtagccg ttttgatagc tgtcttgatt atccgcaaag cacggaaata
     8941 tgtggatctt cagcaagatg aagtggggaa actaaatggt tatatggatg aaaaaatcag
     9001 cggacaacgt gtcatcatca caaatggctt acaagaagaa acgattgaag gattcttaga
     9061 gcataatcaa agtgttcgcg aagcaacctt caaaggacaa gcttattctg gtctactatt
     9121 tccgatgatg caaggaatgt cactggtcaa tacagcgata gttatctttt tcggcggctg
     9181 gttagcatta aatggtgatt tggaacgttc ggtggcatta ggcttagttg tgacattcgt
     9241 ccagtattca caacaatatt accaaccact gatgcagatt tcatctggat acagcatgat
     9301 ccaattagcg attacagggg caagacgatt gaatgaaata tttgatgaaa aagatgaagt
     9361 caaaccagaa aacggtaaag catttgatgg tgttgaaaaa ggtcttgcgt tgcagcacgt
     9421 agattttggt tattctccag aaaaactagt cttgaaagat gtctcgatca atgtggacaa
     9481 aggagaaatg gttgctcttg ttggtccaac aggatcgggg aaaacgacga tcatgaatct
     9541 gctaaaccgt ttttacgatg tcaatgatgg ttcagtagtc atcgatggaa tagatatccg
     9601 agattatgat cttgatgcat tacgtagaca tgtaggaatc gttttgcaag aatctgtatt
     9661 gttctccgga acgatcaaag acaatatcac attcggcaaa ccggacgctt cagatgaaga
     9721 agtcatcaat gcagcaaaac aagcgaatat tcatgacttc atcatgacgt tagaaaaagg
     9781 ttatgataca gagatatccg aagaaaataa cttattcagt acaggtcaaa agcagttgat
     9841 cagtattgct cgaacgatca tcacaaatcc atctttattg attttggatg aagcaacaag
     9901 taatgtagac acggtaacag aagcaaggat ccaaaaagca atggatgaag caatcaaagg
     9961 aagaaccagc tttgtgattg ctcaccggct gaaaacaatc ttgaatgcag atagaatcgt
    10021 tgtcttgcgt gatggggaag tcattgaaga aggcagtcat catgagttat tagcagaaaa
    10081 tggattttat gcggaacttt atcataatca atttgtattt gaaagttaaa ttaaaagaac
    10141 ctcgttataa cggggttctt tttatatatt ttatgttgtt tgtttttata aggggcagaa
    10201 atgatgtgca ccccaaaagt tagactagaa atctaattaa aaggggtgct ttttttgcgg
    10261 aaatatacat ttgtttttaa gaaaaaagta gtttcagact atttaaataa cgaaggcggc
    10321 tacaaatatc ttgcacataa atatcaaata aatcgtacac tggttagaca ttgggtaagg
    10381 atttataact atcatggttg ggaaggcttg gttggaggtg gcaaaagcta cactacaaaa
    10441 tttaaacttg atgttataga atatatggaa acaaatggtc tttctatcca agaaactgct
    10501 aaaaaattta atatcggttc aaatagaact ctaagtaaat ggatagagca atatgaagaa
    10561 ggcggtgctt cttcacttga gagccaaaaa aggggcagaa aaattagtat gaattccaag
    10621 ctaaacattc ctaaaaaact taaagatgag tctcttgaag aagaagttat tcgtttaaga
    10681 gcagagaacg catatttaaa aaagttagaa accttgattc aagaacaaga tttgagcaag
    10741 aagaaatcaa ggttaaaaca atccttcagc tcaaaaaaga atttaaattg aatctattac
    10801 tttcaattgc acaattagcg aagtcaactt attattactg ggtaaaaaaa ttagataagc
    10861 cagataaata tagcaagata aagcaagaaa ttacagcgat tgtaaaagaa tcaagaaact
    10921 cttatggtta tcgtagagtc actttagcgt taaagatgaa aggatataca atcaatcata
    10981 aaacagttag aaaattaatg tcccaaatgg gacttacctg tcaaatccga ataaagcgat
    11041 ataaatctta taaaggaaca gtaggaaaaa ttgccaagaa tgtgttaaaa cgaaattttt
    11101 cagtagatac acccaataaa aaatgggtga cagacgtcac tgaattcaag ataaaaggaa
    11161 gaaaaatcta tctatctcct atacttgatt tatttaacgg agaaataatt agttatagta
    11221 tatcaactag tccaacatat aaactgattg aagaaatgct tcaacaagca attaaaaaaa
    11281 agggaactga gggttcactg attctacatt cagatcaagg atggcagtat cagatgccac
    11341 aatatcaaaa aaaattaaaa gagaataaca ttattcaaag catgtctaga aaaggaaatt
    11401 gcttagataa ttctgtaata gaaaattttt ttggtgtgct taaatcagag tttttttatc
    11461 gagaaaaatt ccgatcaatt gagatatttc aaagtaaatt aaatgagtat attaggtggt
    11521 ataataacaa aaggataaaa ctaaagttaa atggactatc tccagtagaa tatcggaaac
    11581 agtccattaa atagtt
//
LOCUS       DACDYJ010000080        11459 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDYJ010000080 DACDYJ010000000
VERSION     DACDYJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.081"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(75..620)
                     /locus_tag="IWM51_002318"
     CDS             complement(75..620)
                     /locus_tag="IWM51_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9971693.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(632..1324)
                     /locus_tag="IWM51_002319"
     CDS             complement(632..1324)
                     /locus_tag="IWM51_002319"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP9971694.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1343..1627)
                     /locus_tag="IWM51_002320"
     CDS             complement(1343..1627)
                     /locus_tag="IWM51_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971695.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1640..2203)
                     /locus_tag="IWM51_002321"
     CDS             complement(1640..2203)
                     /locus_tag="IWM51_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9971696.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2430..3080
                     /locus_tag="IWM51_002322"
     CDS             2430..3080
                     /locus_tag="IWM51_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP9971697.1"
                     /translation="MIKRNWPWLLALVLLFLFGFSFVLGLLLIGVVLVLWGLFGSRRK
                     KGKQEEIPALTNELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAKLKAI
                     DLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIKNKQT
                     YEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQKD"
     gene            3204..4400
                     /locus_tag="IWM51_002323"
     CDS             3204..4400
                     /locus_tag="IWM51_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP9971698.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4501..5877)
                     /locus_tag="IWM51_002324"
     CDS             complement(4501..5877)
                     /locus_tag="IWM51_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9971699.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIISILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5914..6780)
                     /locus_tag="IWM51_002325"
     CDS             complement(5914..6780)
                     /locus_tag="IWM51_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP9971700.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6869..7894)
                     /locus_tag="IWM51_002326"
     CDS             complement(6869..7894)
                     /locus_tag="IWM51_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9971701.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7912..8586)
                     /locus_tag="IWM51_002327"
     CDS             complement(7912..8586)
                     /locus_tag="IWM51_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9971702.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8770..9663)
                     /locus_tag="IWM51_002328"
     CDS             complement(8770..9663)
                     /locus_tag="IWM51_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9971703.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNDR"
     gene            complement(9737..11434)
                     /locus_tag="IWM51_002329"
     CDS             complement(9737..11434)
                     /locus_tag="IWM51_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9971704.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3394 a         2513 c         1964 g         3588 t
ORIGIN      
        1 aaaacaaagt cgtttgtttt atgctcttct tttattattt tgtacttttt attttatcaa
       61 atcactttgt ttttttataa aatttctgcg cctagtgtgg attcaaaatg tctcaatgcc
      121 cattcatgtc caacgggatc gaaacttgcc acggcatctt tgaaaatatg gagtttgtat
      181 cctagattat acgcatcgac tgctgtatgc aacacacaga tatctgtaca tacgccggtc
      241 aaataaatat ctgtgatctg gcgttctcgt aaacggatgt ctagatctgt tccactaaaa
      301 gcggaataat gccgtttgtc tatccaataa acattttcct cttgctcata gtcttggtat
      361 aaacttttta aagagccgta caattcccgt ccacccgttc ctatcacatt atgcggagga
      421 aataagcggt tctccggatg aaaggaatct tttggatcat gcgcatcaat ggcaaaaacg
      481 acaaaatcct tttgatcaat aaacttcttt gtatgagcag tcagcttttt ttcaattact
      541 tgtcctgctg tcccagtagt cagctttcca tcctctgcaa caaaatcata tgtgtagtca
      601 atcgagatca atgcgttcat cttagtcctc cttatacgag atgtttgaca aattcgatca
      661 ccatttctgc tgatcgtttg cctgcgtctt cgataaattc gtcaaagctt tgtgtcgccg
      721 agtgatctgc tgtgtcgctc attgcacgta cgatcaaaaa tgggatattg aactgacgag
      781 ctgtttgtcc aacagcggct ccttccattt cgcaagctaa tgcttcaggg aaattcgtta
      841 agatttcttt gattttatct ggcgagtcga caaatgtatc acctgtaacg atcaatcctt
      901 cttttgcgtt caaattcgtt gcttttgctg ctttaaccat ttcactgcgc aaatatgtgc
      961 ttgcttcata atatagaggc attcctggta attgtcctgg tttgtagcca aatcctgttg
     1021 catctgcatc aaaataagct actttgtctg atataacgat gtcacctact tgtaagcctt
     1081 caccgattcc gccagctgaa cctgtattga tcaccatgtt cacgccgtat tgttggatca
     1141 acagacttgt ggtaatagat gctaagactt ttcctatacc ggaacgtacg acgatcactt
     1201 cgtggttacc taatgaacct gaaataaata aagcgcctgc gcgttcccat gataatggtt
     1261 cgcttaggtt ctctctcaaa atttttactt cttcttccat tgcgccgata ataccgattt
     1321 tcatcttttt aattccttct ttctatataa atgcgattgc taaaaaaacc acacataaca
     1381 ttaggataac aataataaga gatttcatca aaaaagagtt ccatttggtc gtcttttccc
     1441 gttcgctgat ccttgttttc gtgattggtt tattcttttt gctttcttta cgataaaagc
     1501 tggcaatctt tttttcttct tgctgataag ctgtttctgc ttttcgctgt ttttttaatg
     1561 actcgcttgc ttgtgcttgt tgtcgcttgc gaagttcgct tcgcgtaata agcggtcctt
     1621 tggccataag ccgatccctc tactttcctt tggtcaatag ttcccagtat tgaatcgcat
     1681 aaatcgtctt tgcatcacaa atcaactgat ctttcatcgc ttgttgcgcc tcttcaagag
     1741 ttagatgata caactccagt acttcgtctt catcttgtgc taaaggattc tcgaccttct
     1801 ctaccccttg tgcatgataa atatgaagga cttcattagc aaaacctggt gaaagataca
     1861 tcgaagtaag atggctcagc gacttcgcac gatagccagt ttcttcttct aattctctag
     1921 ctgctgtcat ttcgggattt tgtccttctc ctggatcgat ttttccagct ggaatctcca
     1981 aaatcacttt ttctaaaggt tttcgaaatt gcttgacaag aagcagacga tcttgttcgt
     2041 caaaagcaat gatcccaaca ccgccaggat gaaataccag ttctctcttc gaagttcctc
     2101 catctggcaa acgaacttcg tctacagcaa catcaataat ttttccgcta tagatttctt
     2161 tgcgactgat tgttttttct tcaaattgct cattgttcag catatcttct cctgctttct
     2221 ttgtaatcat aactgctttt tttcattttt caatttttct caaaaactcc ttattagttt
     2281 accatagata aaccttagat tttctctaaa tatagaaaat ccttctttag tccgatactc
     2341 tttctagtag tccgtatgct aaaataagta tgtaatcgaa taacgaaaag aattcaatac
     2401 tcaacgataa atgacatgga gggtttatta tgataaaacg aaattggccg tggcttctag
     2461 cattggtctt gctatttctc tttggttttt cttttgttct aggactttta ttgataggtg
     2521 tagttttagt tttgtgggga ctctttggtt cgcgcaggaa aaagggaaaa caagaagaaa
     2581 ttcctgcact gactaacgaa cttgaaactc actatgaaaa aagcggtatg acagcgaatg
     2641 aaatttcatt ttttcgacaa acaatgaatc aaacaaaaaa tgagatcaat caattgcaaa
     2701 tgaatatgca gcagacagcc aaacttaaag caatcgatct ccgccatgat ccagtcaaag
     2761 cagcaaaagc tttattcaaa gaattggtga aagaaccaaa ccgactgcat gaagcaagtc
     2821 agtttcttta tacgcatctg ccgaatctag ttgatttgac aaataagtat aatgagatca
     2881 atgaccatga gatcaaaaac aaacagacct atgaaaaact agaagaaagc gcgcagatca
     2941 tcgaccagct gtcagcttta atcgcacaag attatcaaaa attcgttgct gaggatctag
     3001 atgatatcga cgtagagata tctgttgcga aacagagctt aaagcgtgat aataaatatg
     3061 aaccagaaca aaaggattaa ctcttttgtt cataaaagaa gactcggaac attcagatga
     3121 tgtctggctg tttcgagtac tttttctaaa gtagagacat tcatgtaaga atcgagaaca
     3181 gattcaacaa ggaggaattt tttatggatc ataaaccgac tgaaacaaaa gatgttacac
     3241 cagttaacga tacacttgaa gatttattga acaacccgtt ttcaactcca acagatacgc
     3301 tgaccacttc acaacaagcg gagattgatc agctccaaga gcaacagaca gctgctcgtt
     3361 taattgacaa actaccggcg gaacgccaag aacaagctaa acagctagca gcaaaaatcg
     3421 atgctaatga tgcacagtct gtcatcagct acggatcggc tgcccaagca aaattaagtg
     3481 aattctcaca atcgatgttg aatcatgtgc aagcacaaga tattggtcct gtaggagatt
     3541 ccttaactga actgatgtat cgtctccaag aagccaatcc tgacgaactg cgtgccggag
     3601 aagggaacat cttccagcgt gtcttcggga aggtcaaaca atcgatctat gaagtgacag
     3661 caaaatatca aaagattggt gcacaaattg acaagatttc agtgaaatta gataaagaaa
     3721 aagacggtct gctaaaagat aatctgatgt tagaacagct ttaccagaaa aacaaagact
     3781 actttgatgc actgaatatt tatatcgctg ctggagaatt gaaaatggaa gaacttcaaa
     3841 caacgatcat tccagaagcg atgaaaagag cagaagaaac tggcgatcag atggatgttc
     3901 agattgccaa tgattacacg caattcctag atcgtctgga taaacgtacg catgatcttc
     3961 gcttagctag acagatcacg atccagcaag caccacaaat ccgtttgatc caaaacacaa
     4021 atcaggcctt ggctgaaaag atccaagcat ctatcgctac agccattccg ctgtggaaaa
     4081 accaagtcgt gatcgcattg accttgcttc gtcaaaaaga tgcagtaact gctcaacgcc
     4141 aagtctctga aacaacaaac gacttattga agaaaaattc agaaatgctg aaaatctcag
     4201 ccatcgaaac agcgaaagaa aatgaacgtg gaatcgtgga tatcgagacc cttcaaacta
     4261 ctcaaaatga tttgatcgaa acaatccaag agacattgcg tatccaaaaa gaaggaaaag
     4321 aaaaacgtcg tcatgcagaa gtagagcttg gacacatgga agaagatctg aaacaaaaac
     4381 tgttggattt aacccaatag atcacctttc gcttgttact aaaacaaacg cccaacaaca
     4441 gtttattaca actgttgttg ggcgtttttc ttatctttct tctaacttct ccattggagt
     4501 ctatttctca ttcatcttgc tgtgtccata gccatagcca aaataaatga tcagtccaat
     4561 aaccattacg ataccgaaca cgatccatgt cacagtttgc agacgaagca tcaatacaat
     4621 acaagtcaag atggaaatga ttggcaatag cggaacgaat ggcactttga attctccagc
     4681 ttttggtttg ccaaacattt ttctcaactt tagtaagcct agactaacaa gcatcaaata
     4741 ggcgagcgcc atgatattcg ttagttccgc taatacattc aatgggaaaa atccggcaaa
     4801 gaatacagct gccacaccag cggcataagt cgcttttttc ggtgtacgat gcttttgatc
     4861 gatttctgtc ataaacttag gaagtaagcc atccttgctg acagcaaaca gtaaacgagc
     4921 aagtgaatac atcatcgaga ttgttaccgt cagtagcgtt aaaatggccc ctacagcgat
     4981 caccgcgccc actgttccat gtcctacata cctcattgca aaagccactg ggtcattgac
     5041 tcctagttca gtatacggaa cgattcctgt caagatcagc gtcacgatga tgtaaaggac
     5101 agtagcaatc agtatcgaac caatgattcc tcttggcaca tctttttgcg ggtttttgac
     5161 ttcctcggct gccattgcaa cggcatcaaa tcccaagaaa gcaaaaaaca caagagctgc
     5221 accatctagc acaccggaaa aaccaaaagg catgaatggc tgccagttat caggtttaac
     5281 gaaaaatatt cctacaatga taaacaacgc gatgatacca aatttgacat ataccatggc
     5341 attattcaaa cgtaatgctt tcttggcttc aagcgacacg atgtaagtga cgaatatcac
     5401 tacgagcacc gcaatcaagt caatgtatgt accattttcc ggattgtacc cgccagttaa
     5461 tgcttgtgga agttcggccc ctaaactttt caaaaaacct tgcatatagc cagaccatcc
     5521 ggaagcaaca gaggaaaccg ctagtaaaaa ttccccgatc aacaaccacc ctgtcagcca
     5581 ggcagcaaat tcaccaaata caacatacag ataggcatat ggcccaccaa tcactggcac
     5641 tcgcgaagca aattctgcga aacttagtcc agacaaaatg acaacgattg cagctaagac
     5701 aaatgaaagt gacagtgctg gaccagcatt ttgattggcc gcaacccctg tcaccacaaa
     5761 tattcccgta ccgatgatcg caccaattcc aagcatgatc aaatcagacg tttttagttc
     5821 ttttttcatc tcactcgtat gattgagatt gatctctttc ttacgaaaca aatccattga
     5881 agttcctccc cattttaggt tggaaaacag cgattaaact gttttcatat accctaaaag
     5941 gataccacct aaaatgacta aaaggcaacc aacaataaca taaatgagct cttttttcgt
     6001 ttttctttcg ccaaggatga aaatcccacc taaagtagag ataatgatcc ccatttgtga
     6061 aagtgagaag ccaacagcta aaccaagtga tttgactgtt agaagcatgc agatgtttcc
     6121 taagccccat aataatccac agatcatgtt tttccacaca aactgatcga ctttaacttt
     6181 acggaaagca aagaagcttg cgcctaagat cattccgata ctttgaggta agataattga
     6241 catcgcatcc aaattcgccc agttgacgat gatggtatat acaccataac caatcgtaga
     6301 gaagatcaat gctctaaaac ccttaccaaa atcaagcatt ttattatctg tatctatttt
     6361 tccttcgtca tcttgtcggg cagtcaaata cgcaccgctt acaagaagag ctaaagcaat
     6421 aataccgtat acataatctt tcgtttgtgt ccattcatgg aagaaaacgg ctcccgcaat
     6481 cgtattcaaa attaattgaa aaccagtaga caatggtagc cctacagaaa cacccatgta
     6541 cttcattcca tggaactgtc cattttgtcc aacactccaa gctaagccgg ataaaaatcc
     6601 gatcaagaaa atccaagggg tgattacagg actaacaacg aaaaagacaa ctaaagaaaa
     6661 cagaaatgca ccgatcgtca ttcctagtgt ttgttgattg gcatcgccac caattttacc
     6721 acttactaaa ccaatactac cccatgccaa catagggact aatgctatta aaatttccat
     6781 aaccaatctc cttttatttc acatgataag aaacttatca gcgaaaacgt taacaacaca
     6841 agcacttttt ttcgtttcta acgattttct aacgtttttc attataaaaa tttaagattg
     6901 ggaaaaataa agtcattgtt gtcccttttc cttcagttga ttcagccgtc aaatggatac
     6961 ctaatttttc ggccagattt tttgctaaat aaagtcctaa tcctgttgaa tgtgttttgc
     7021 taagtcgacc atttctccca gtaaatccct tatcaaagat ccgtttcaag tcttccgctg
     7081 ggataccgat ccctgaatct ttaactgaaa ggctgactcc ttgcgcttcc ttttctatgc
     7141 tcacaatgat gtttccgtga tccggcgtat atttgatggc attactaagc aattggttga
     7201 agataaaagc gatccattta gcatcggtca aaacagtatg atcctcacca acgatctcgt
     7261 agcgaatatt tttttggata aaataatttg cctgactgcg tatcacaggc tgaatgattc
     7321 cttttagtcc atattcttgg atgaggtagt cccgcgagaa actgtcgagt cttgcataat
     7381 acaaaacttg ttctacgtat tcatcgattt ttgataattc attttctagt aatgtatact
     7441 tttgatcgtc aatatcgaac tcgatcgaac gaactagcaa tttagaagcg gaaagtggca
     7501 ctttgatttc atggacccat gagtcaatat aatctttttg gtcttgttga ttactgattg
     7561 ctgattgcat tacttcttgg tgttcacgga tcagctgatt gatatattct tctaccagtt
     7621 tttcttcttc tgtcctccca ccactcaaat aattttgcaa aggggaagtt ttttgtatgt
     7681 cggctaattt tccccaccat cttctcttgc tcaagtaacc acccagcaga taaaaaatca
     7741 gaaagacgcc ctcaatcaaa gctaaataag caattgtgct caaatcaact ttgatgtttg
     7801 gcgccagcca catcacaaag atcgtcaatg caataaaaaa cagccatccc aagatcaatg
     7861 tccactgatc tttcaaatat ttaaaaaaat tcatcttaac cgctcatcct ttcagggaac
     7921 gatatatccc tgtcctactt ttgtttcgat atatccttgg atacctgcct gttcgatttt
     7981 tcggcgtaac cgattgatgt tgactgtcaa tgtattgtca tctacaaatc gttcgtcatc
     8041 ccacagtgct cgcagcaatt tttcccgact taagatcttt ccattgtgct tcatcaaaat
     8101 aaataataga cgatattcat ttttgctcaa atcaatgatc tctccgttga tttccatact
     8161 tccgttgtca acattcaacg taatgccatt gtggctcatc atttcactag ataattctgt
     8221 gtagttgtaa gaacggcgaa gtaatgcatt gatttttgca atcagtacat cgatttggaa
     8281 atgttttgtc acaaaatcat ctgcccccat gttcatagcc atgatcatat ccatattcgt
     8341 attcctgctg gagataaaaa tgatcggtac tttcgatact tcacgtattt tttgacacca
     8401 ataatagcca tccaaaacgg gaagattaat atctagtaat acaagctgtg gttcttctct
     8461 ttcaaagtct gagaacactt gattgaaatc ggtcgttcca aaggtttcaa attgccattt
     8521 ctcaagttcc tccatgatca attgtcgaat gatctcttcg tcttcgacta ccatgatttt
     8581 tgccataaaa aatccctcca tctttcttct tgctactaca atatcaaaga agcccttgtg
     8641 aaacaagttg gcttacctgg aaaatgtcat tttagcaaac gttaaaaaaa actggaaata
     8701 tgcgatttaa tcgcgtttgt ctcttccaag cagcttaaat ttattatgtt attcttaata
     8761 attggtctat tatctgtcat ttttctttcg ttgtttttgt cttttaaatt ttcgataaca
     8821 ccaataaccg aaaagcgtta gcactagcaa tatacctaga aggagtataa gtaatcgata
     8881 aagatggtat ttttttgtct gttctatctt tgaagagact tcttctgtaa agggaactcg
     8941 tcttcctgtc actagtaatc ggtgagtgtt cacggtgtaa ggtgtacaag tgataagtgt
     9001 gactagatcc tgcttttctc taattgctaa tgtatttgtt tttgttggaa ggacaacaat
     9061 tttctcaacg acttcataag cgagcatccg accatacact tcaagataga atcggtcatc
     9121 ttgctccatt tcttcaagat ccgtaaagag ttttttctca gcaataccgc tatgggcggt
     9181 aatcacggaa tgtgtactct ctcctccagt tggaaaagag gtgccttgca ggactgtcgc
     9241 tcctctatct aacaaaagat cattcgtttc atcaaataaa ggcaagctaa cattgatttt
     9301 cggaatatag atggctccga tcatatgttg ttcgtaatat tcttttcctg gattttggac
     9361 atttctaacc gcttgctcaa aaggatcttt gacttgtccc attcctggga ttactgatgt
     9421 atgagcaagc gtctgatttt tcttttcatg cactgctaat tgttgctcat gtttttcttc
     9481 tttatcgcgt tcaagctgct tttgataatt ctcgatcctt ctttgatcca aataattgct
     9541 gagagcatct gctaaaaatg gatatgtaaa aactgcagct ccggcaagaa acatcactgc
     9601 cataaataat tgtaataaaa gactgatttg ggtttgaggt ctattaccta ttttcttttt
     9661 cattttcttc accttcttat ttaaaaaaac tgcagcaacc tttataaaac cgattgctgc
     9721 agcttatttt tgttgcttat acttcagact tcattttaga tttacggtac cagctgtacg
     9781 ctccaatcat caaacttatt ccgattagta agaatgccaa gagcccgttc ccaccagtag
     9841 caggaagtga cccgcgtttc atattcggta ctctttctgg ccctggattt tctactccta
     9901 cgatctgaat ttcttcatta tagcttccat aagagatgat aaattcagta aaggctgcct
     9961 cacctgttgg aagtacataa ccttctggcg ccatggtctc tttcatttga taggttccat
    10021 aatctaaccc ttgaacctca aagtatccat ttccatctgc gacaaattct gttgcttgtc
    10081 cgcttgttgc ccactcaatt ctatctgctc ctgattcata aacagctaaa ggcgactccc
    10141 ctttataaaa tattgcgtat tcagaacggt cttcattcca caattcgaat cttgcatctt
    10201 taagcaattc actgcttgat gcatcatatt tttcaaactt tcttccacct gttgtgactt
    10261 tctctggcgg ctctactcct tcttcataat caaattcatc atttcgtccg atttcaataa
    10321 ccgctgtgtt gttatggaaa tcatccgctt ctagttctgt ggaaaccttc aaattatacg
    10381 tgatcgttaa atctcctcca cgtgcatctg ctaatctacc taaagtatct actttggcta
    10441 ctccattttc ttctgtcaaa atcgtcacga caaacccgtt gcttactact tctactatat
    10501 aatcttgtcc ttttgttaaa atcgtctcgc cggctcgcac ttctatttcc tgatcataat
    10561 aagttagacc aactgtaggc gtatctgtga ttttgtaatt tttgataaca gcgcttccgt
    10621 tttcaaggac agaatcgatg aaataaggaa taggtgctgt gatcgtgtaa gaagccgttt
    10681 ttcctctttc tagatcaata taagaaatcg tttcttgttc tccagcttca ttaataatcg
    10741 ttacttgtct aaggtcgctt tcctctacat tcatctgttt atctgcttct ctgatttcgt
    10801 ttttcggata caaatgcaca tctgtattgt atgtgtcttt ccatgtagaa ccatcccaca
    10861 cgccatccac catatcaggc atcatcactg gcatcataac aaccattgga gacgcgattc
    10921 tggaaatatg agctggcgaa taagtttcta agaaaagata gactttatct ctagaagaag
    10981 gtgcttcttc cgtcacttgg acggtagaga ataccgcttc tccagcttca ttcgttgttt
    11041 gtttgtcaat acttgtcagt cgatatttta aaaaccaact ttctgtgtgc cgtttttgaa
    11101 tccattcaat cgcttcggca gaattaagac cgtcttctcg ttccggtgtt aaaggattat
    11161 ctcgaatcaa atcgtaatat acttccgtta cgtcaaacaa atcaaaatcg actcctggaa
    11221 tattttcttc accaaaatcc gtagaaacca atccgctatt cggtcgttct tccggaacag
    11281 atgagaaacc ttttttgtgt aatgtaatcg tcacttcact tggtttttgt gcgacttctt
    11341 cggctttgac tagttttccg ttcccgaaaa tccctattcc cgcagtaacg agtaacagta
    11401 cactcattcc cgtgacaatc atttttttat tcattttttt ctccccgttc tttttttat
//
LOCUS       DACDYJ010000081        11254 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDYJ010000081 DACDYJ010000000
VERSION     DACDYJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.082"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IWM51_002330"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IWM51_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP9971705.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IWM51_002331"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IWM51_002331"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP9971706.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IWM51_002332"
     CDS             complement(2169..2906)
                     /locus_tag="IWM51_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9971707.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IWM51_002333"
     CDS             complement(2997..3524)
                     /locus_tag="IWM51_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP9971708.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IWM51_002334"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IWM51_002334"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP9971709.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IWM51_002335"
     CDS             complement(4401..4886)
                     /locus_tag="IWM51_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP9971710.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IWM51_002336"
     CDS             complement(5039..5236)
                     /locus_tag="IWM51_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971711.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IWM51_002337"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IWM51_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP9971712.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IWM51_002338"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IWM51_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP9971713.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IWM51_002339"
     CDS             9211..9423
                     /locus_tag="IWM51_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP9971714.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IWM51_002340"
     CDS             9425..11104
                     /locus_tag="IWM51_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9971715.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3265 a         2429 c         1971 g         3589 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaat
//
LOCUS       DACDYJ010000082        10889 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDYJ010000082 DACDYJ010000000
VERSION     DACDYJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.083"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..660
                     /gene="tnpB"
                     /locus_tag="IWM51_002341"
     CDS             <1..660
                     /gene="tnpB"
                     /locus_tag="IWM51_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP9971716.1"
                     /translation="YISLLCKEEVRELPKSNSAVGIDLGIIDFAILSTGQKFDNNKFT
                     SKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEKVMNQRTDFLNK
                     LSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKADWYGRKIIKV
                     DKWFPSSQICSNCGHKDGKKSLEIREWTCPICHTHHDRDINASINILTEGLRLHSMGL
                     A"
     gene            complement(1010..1852)
                     /locus_tag="IWM51_002342"
     CDS             complement(1010..1852)
                     /locus_tag="IWM51_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9971717.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2177..2593
                     /locus_tag="IWM51_002343"
     CDS             2177..2593
                     /locus_tag="IWM51_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9971718.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIRESQQSVIDCRKLLVEQLTGGDDL"
     gene            2590..3060
                     /locus_tag="IWM51_002344"
     CDS             2590..3060
                     /locus_tag="IWM51_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9971719.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3074..3847
                     /locus_tag="IWM51_002345"
     CDS             3074..3847
                     /locus_tag="IWM51_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9971720.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            3861..4673
                     /locus_tag="IWM51_002346"
     CDS             3861..4673
                     /locus_tag="IWM51_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9971721.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            4727..5692
                     /locus_tag="IWM51_002347"
     CDS             4727..5692
                     /locus_tag="IWM51_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9971722.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            5829..8552
                     /locus_tag="IWM51_002348"
     CDS             5829..8552
                     /locus_tag="IWM51_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9971723.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8565..9197
                     /locus_tag="IWM51_002349"
     CDS             8565..9197
                     /locus_tag="IWM51_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9971724.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10096..>10889
                     /locus_tag="IWM51_002350"
     CDS             10096..>10889
                     /locus_tag="IWM51_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9971725.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3667 a         1692 c         2112 g         3418 t
ORIGIN      
        1 tatatttctt tgttatgcaa agaagaagtt cgcgaactac ctaaatctaa ttctgctgtt
       61 ggtattgacc taggtattat agactttgca attctttcta ccggtcaaaa atttgataat
      121 aataaattta cgtccaaaat ggaaaagaaa ctcaaacgtg aacaacgtaa gttgtcaaga
      181 cgtgccttat tagctaaaca aaaaggtatc aatctgtttg aagctagaaa ttatcaaaca
      241 caaaaacgta aagtagcacg attgcatgaa aaagtaatga atcaacgtac tgattttctg
      301 aataagttga gtacagaaat tatcaaaaat cacgatatca tctgtattga agacttaaac
      361 acaaaaggca tgttgcgtaa tcataaacta gcaaaaagta tttcggatgt ttcttggtct
      421 agttttgtgt ctaaattaca atacaaggct gactggtacg gtcggaaaat catcaaagta
      481 gataagtggt ttccatctag ccaaatctgt tcgaattgtg gacataaaga cggcaagaaa
      541 tctctcgaaa ttcgagaatg gacttgccct atttgtcata ctcatcatga ccgagatatc
      601 aacgctagta tcaatatcct gaccgaaggt ctaaggctac actctatggg attggcttag
      661 atcaaataaa caagaaccgt aggaactacg gggatagctt ggtaaataag aggcacctct
      721 gttagtaaag taatacgcta tcaagtatgc tctcttccca agaagctccc acttcaagcg
      781 taagaaaccg ttaggtttag ctaagtggtg agtagttcac taaggaagta aacccacaca
      841 gaaatatagg tgattttcct tgaatgacca agatattgaa agttagtgaa aaaacacaaa
      901 aaggtagccc tattcacaga aggtctgcct tttgtgttat cgccagcaaa cattagaggg
      961 gatatttcat atagcttttg tcctctcgac agtcctatca gtttctcttt taacttatct
     1021 ctaagtgctg tccagtttta atagcttcta gcttgtttcc taactgtgtt tttaatatgt
     1081 tgaaggcttt tgaacctgtg cagtggcaag taaggatttt ttctactcgt gagtcattca
     1141 atttatttcc aattttcatt acctcttgct caggtagacc taaattattt atataaggta
     1201 tgccaatcat atggaagcca ccgattaggt attttatggg tttagtcgga aatgtttcaa
     1261 gagcaatttc catcatatcg ataatgccat gatgactaca gccagtaaag acaatcagac
     1321 cgtcttcttg ctcaattacg accaataatt catctgaaaa atcatcataa acaaatttat
     1381 catccatctt tttgtataaa ttcttggtgg tcgagtttgc tttgcctagg gggatgatat
     1441 ggaagctcgg agcaagttct gttttcgttt ttattgtatg taaattctca tgcattttaa
     1501 ggatttcatt tttaggaata ccaacataca gaaacgtggt taaaatcttt aaataatggg
     1561 gtaaagttac accttcagct gtataaatag gggcgtttcc tgattttaaa tatcttaacc
     1621 cgccggtgtg gtcagaatga ccgtgtgaaa taatcactgc ctccagatca ttcaaagttt
     1681 cccccatttt agtcgcatta tagactaatt ttttattagc gccagcatcg aagagatatt
     1741 tcttccctga gtagttaatt aagagagata acccatgtcc acttttaaag ttcaaattta
     1801 ttcttgagtt ttcaaataaa accttgattt ccactataac atctcctttc acatgaacgt
     1861 gtattcatat attgtagcac attaagttct attttttgtt gtaattaccg aaaaaaatat
     1921 taatggacgc tttttacgct ttatttcgta tgtggaagaa aatttcttag gatgtggcgg
     1981 gggattgggg caaagctaaa ttccaattac agtgatcatt taattagaca cctttaaata
     2041 cccacacttt catcaacact ataatttagg gaaagaaaag tgttggcatg atatagaaaa
     2101 acctttaatt gacagcgttt cctaaaattg gcacgcatct tgcttataga aaagcgtaag
     2161 gataggagga atgaaaatgg caactataat tcttgccagc catggggaat ttgctcaagg
     2221 attaaagcaa acagcgacaa tgattattgg agatagtgta ccaattcatg ccttgtcagc
     2281 atttagagat gaagatgaat ctattttggt acaaataaaa aaacttttag ccacaataga
     2341 tatagaagaa acctacattt taactgatat tctaggtggt agtgtgaata atgatatgct
     2401 cactattatt aaagagtatg agggattgca attaattact ggaatgaact tacctttagt
     2461 tatttctatt gcaacctata atggaaaaat tggggaaagt gagctagaaa agcttattag
     2521 agagagtcaa caatcagtga ttgattgtag aaaattatta gtagaacaac ttactggagg
     2581 agatgaccta tgattaaatt agtacgtatt gaccatcgtt tgttgcacgg acaggtagta
     2641 ttttcttgga gtaaatcatt aaatatcaat cgtattatta ttgccaataa agaggcagcg
     2701 aatgatgaat tcaaaaaaat gtctttgaat ttatctaaac cgactggcat taaattaaca
     2761 attttttcag ttgaacagtt actagaaaaa atgcctaaaa tcgaaacact taacgaaaat
     2821 atcatgctta tttttggtaa tacaaaagaa attcgtcaat tttgtgaagc ctatcctaaa
     2881 gtacaagaaa tcaactatgg agggataatc aaaaaagaag gttccaagca atttagtaat
     2941 gccattttct tgaatgaatc tgagcaagaa gatgccaaaa aattaaaagc tcttggcatt
     3001 aaacaattta ttcaacaggt accaacttct aaaaaagaag atttgaatac aatgttataa
     3061 aggaggaatg aaaatgctcg ttcagtcaat tggtttaggg ttaatcggtg ttttttgtat
     3121 tttagactca aggcttttag gaagaatgaa ttttgaacga ccgttgattg tcagtacgct
     3181 tgttggtttg gtacttggag acatggaaaa aggattaatt gttggagcct ctttggaatt
     3241 gatgtcctta ggattagtaa atatcggagc tgctgcacca cctgatatga atatggcatc
     3301 aattattgct acagcctttg ccattttatc aaatgcgaac gcagaagcag cattaacgat
     3361 tgccattcca atttcagtgt taggtcaaat actaggtatc ttaatgcgga cgattttgtc
     3421 aaatttgact cacgttgcgg atcgctttat cgaacaaggg aaatttagcc aagcaaggag
     3481 aattcatata ctttatggct cgggattata tgctctcatg tattttattc ctatttttct
     3541 agcaatttat tttggaacga atcttgtttc tcagattgtt gatgcgattc ctgagtggct
     3601 aacaaatggc ttaacggttg caagtaaaat tttaccagca tatgggtttg cgttgttatt
     3661 acaaacgatg ttaacgaaaa aaatggtgcc atttttatta cttggatttt taattactgc
     3721 ttattctgga ttgggaatca ttggtattgc agccttcgca tgtattgttg cgtttgtcat
     3781 ggttcaagtt caaggtaaaa actcaaatgg taaccaaaat gagccagatc cattagatga
     3841 cttataagga ggtagattaa gtgaaagaga aagatcaaaa tgcatggaaa tatcggcagt
     3901 tcttatggcg ttcatgggca attcaagcct catggaacta tgaacgacaa atgaacatgg
     3961 gttttatgta cggaattgca ccaattctag ataacattta caagaatcca gaagatgaag
     4021 caatgaagaa agaagcttac gagcggcata tgatgtacta caactgtacc cctcaaacaa
     4081 gtgcatttgt gttagggtta actgcttcta tggaggagca atactacgaa gaccaagaaa
     4141 actttaatcc ggaatcaatc agtgcagtga agacttctct gatgggaccg ctatctggtg
     4201 ttggtgattc tttctttcaa ggaacgattc gggtattagc ctttggacta ggaattaatt
     4261 tagcccaaca aggtagtatt ttaggtccaa ttctagcaat gttaatttcc tttgtacctt
     4321 cctttttagt tacttactat ggtgggaaaa ttggctataa catggggaat aaatacttgt
     4381 ctaaactgta tcaagaaggt ttgatggata aagtaatgta tgtttgctcc attgtggggt
     4441 tgatggtcat tggttcgatg attgccagca tgatcgggat tacaacacca attcaaatgg
     4501 gtaaagcttt tgtcttacaa gatgtattag atagtattat gccacaattt ttaccattag
     4561 gattaacctt cttcatgtac tggttattgc agaaaaaggt caaaacagga tggatgttgg
     4621 caatctgttt agtcggagga attattttta gtttgttagg tatcttacaa taagggaata
     4681 accaataaat ttggttgaat aatataaata aggaggaatt caattgatga attcaaaaga
     4741 aattgttaaa gaaatcgtag cgaaaaaaga ggtaaaatca atcgtatttg ttggctgtgg
     4801 cgcttcaaaa gcggaccttt atcctgcaaa gtattttcta gaccaaaatg cgaagcagtt
     4861 acggattagt cattacacag ccagcgaatt taactatgca acaccagcat ctgtagatga
     4921 gtcaactgtc gttatttcag cttctttagg aggtacgact ccagaaacag ttgaagctaa
     4981 cgcagtagct aaagaaaaag gtgctacagt tattagtcta acacgaagca ttgactcacc
     5041 gttaacaaaa gatgcagatt atgtaattta tcatcgattt gcagagaact atgctgctaa
     5101 attagaaaaa acaggctact gtttagaatt ggcagtagaa cttttacatc aagtagaagg
     5161 ttatgaccat tatgaagcaa tgcaagaagg atttgctact atctatgatc ttgcccaaaa
     5221 aagtgcagaa tcagctcgta aagatgcgaa gaaatttgct gaacgcttta aagatgaaaa
     5281 agttatttat gtaatgagta gtggtgctac acatgaagta gcatactcaa catcaatttg
     5341 cttgatgatg gaaatgcaat ggattaattc tggtaccttc cattcaggag aatttttcca
     5401 cggaccattc gaaattgtgg ataaagacgt accgtttata ttattaatga atgatggaaa
     5461 aactagaaat atagatgctc gagcattaac tttccttgaa cgctttgata cgaaaacaga
     5521 agttgtagat gccttggatt atggcttgtc ttcacatatt gctaaagaag tcttagacta
     5581 ctttaaccca ttcgtgatta ctgcagtatt tagagtttat gcagaagaat tgtcagaagc
     5641 aagagaacat ccattaacta agagacgtta tatgtggaaa ctaacttatt aaaaaataga
     5701 tcaaactaaa taaggtggct tgagaaatta agccaccttg ttttcttcaa tgtgatttta
     5761 tacatatggt aaaataacta gtacatatgg taaaataact agtacatatt gtgaaagcag
     5821 gtggcaagat gcagcgatta aatgactatc tttatcaact aacattgctt ttgaaatcta
     5881 cgccttcaga taattattca gctcgcagta tttcgcaagc tttacagttg aatagaagta
     5941 caatcagtag ctacttaaat gaaggtgttc gtgaagggct tttcataaaa gtaaaaagtt
     6001 acccggtttt atttttacat agaacagctc ttgaagaact acatatcaca ctgaataact
     6061 ccgaaataga atcaattgaa agcttgttaa caatttctca aaaacccgca ttagatcaag
     6121 taattggctc taaaggaagt ttgaaagaag caattgatca aattaaaacg gcagtcttat
     6181 atcctggaaa agggttgcct ttattattga tcggcgctag tggatcaggg aaaacctttc
     6241 tagcgaataa aatatatgaa tatgcagtag aagaaaaggt cattcacaca tcggcacctt
     6301 tcatagatta taattgtgca caatatgtga gcaatccaga gttactatcc tctgctttat
     6361 ttggctacac gaaaggagca tttaccggag caagccagga gcatactggt ttgctagaaa
     6421 aagccgatgg aggagtattg tttttagatg aagtgcatcg cttatctgaa gaagggcaag
     6481 agaaattatt tacatttatg gatacgggtg aattctctcc tatgggggat aatagtattc
     6541 ggaaaaaagc cgatgttcga ttagtctttg caacaacaga aaatatatat acgactttct
     6601 taccaacttt tcttaggaga ttgccagtta ttgttaatct acctagattt caacaacgtc
     6661 cgtcgtttga acgtctgtca ttgattgatg aattttttgt ttctgagagt aaaattttag
     6721 ctaaggaact atctgtttct gatgcattga ttcatttctt aatgaatagt gattttgaag
     6781 gtaacgtggg gaatattaaa aatattatca agtatgcttg cggaaacgca tatatccacc
     6841 agaagagtca gcaaacaatt caagttaaat tattggatat gcctttagag tacagttata
     6901 aattcaagga gcaactaaat aaacccagaa agaaaagagc agatcgaatc tactatccag
     6961 aagaaacacg acaagtgcaa ctagaaagaa acaatcagct ttttgttcaa ttttttgacc
     7021 aattacttac tggattctct gaagtgattg aaaataacaa ggatattaaa ctatttttag
     7081 aagaaatggt taccaaggta acacaagtaa tggatacgct tatttttcaa gaggattacg
     7141 aaaaagagca gtctttatac gccattctaa actatcatat tcgtcaaagt attgatttta
     7201 tgcgagaaag ctacgggttt gaacaagatg gtaaccgggt agtcgctttg gccaactatc
     7261 tatatgtcaa ggataatcaa gtgatttttg aaaatagtct tcaagaaaag aaaaccaaag
     7321 atcagcttaa cgaatttttt gctaatgttc ttgaaacccc ttattggtat gcaaaaaaaa
     7381 tcttaagcct tttatctgag tgtttagatc aacctgtgta tgaagaggac attttcttta
     7441 ttacgctata tttttatagt ttgaatgtca cgaatgttaa aacaggtatt aagagtattg
     7501 tgctagctca tggttattct acagctagta gtatggcgaa tgttgccaac cgtatgctga
     7561 ataagaacgt ttttcaagct tatgatatgc ctttagatat tacattagag aaaattgaag
     7621 caaaattgat tcaattcctt ggagattatc gaacagattc aggcttgatt ttattggtag
     7681 atatgggttc actaaaccaa ctaggcggtt tattagtgaa tcatctaaat ggaccactgt
     7741 taatcattga tcatatcaat acaccgttat tattggaagt cggccaacaa gtattaaaca
     7801 ataaaagtat caccgaaata ggagcagaaa ttgaaacaag tcttactttc aaaaagcagt
     7861 tactacttcc caagaaaaag aaaaagaaag ccatcattac ttgttgttat acagggattg
     7921 gtagtgcgac ccagattcag gagattttaa cgaaatgctt gaaagatgca gttagagaat
     7981 tagtaattat tccgtatgat tacaaaaagc tgttagaaaa caagaatttt gagactcctt
     8041 ttcaattata cgatgtcatc atgattgtag gtacagaaga tcccaaaatt aatcagattc
     8101 catatattgg attagatcgc ctgattaatg gggaagcaat tggcgaattt gttcattact
     8161 tggaaaaaga gctgactatt gaagtgaacg atttacaaaa agaattaatt ttcaattttt
     8221 caattagtaa aattgtcgaa aatttaacca ttttggatcc agataaggtg ctatcttttg
     8281 tacaaaaagc aattaaagaa ttatcgaagt tattaaattt tgatttttct aataatcagc
     8341 tgtttttgtt gtaccttcac tgtagttgta tgattgaacg aattgtacgt aaagaaagtg
     8401 tggatgagca agaggacatt gaagaatata tgcaagcaaa tggagaaata atggataaaa
     8461 ttcatagcgc tttttttgag attgaaaggg aatacacaat tgaaatacca gatttagagt
     8521 tgcgattatt gagcgatatt gttcaaggat aggagtgaat tcgaatgaca atgggagtaa
     8581 tttttgatat ggatggggta ctcatagaca gcgagacatt ttattttgag agaagaatga
     8641 acttctttca agaactggag atagatccag gatcaaacca gattgatgat tatgtgggga
     8701 aaacagaagc tgcaatatgg gaaacattag ttcctgagga caaatcatta agagaggaac
     8761 tttatccgaa atatgtagat tatcgaaaaa cgcatccaat tgatttcgct aaagatttac
     8821 gaaaagaagt gaaaccgtta cttgacttcc tcaagaagcg ggatatacca ttggcactgg
     8881 catcttcttc tccagttgtt gagatagaga gtatgctcat tcaatgtgaa cttactgaat
     8941 atttttcctt tgttataagt ggtgagtcat taaaacaaag caaaccacat cccgaaatat
     9001 atttaaaagc gaaacagcaa ttagactgtg acttctttat tgcagtagag gattcaacga
     9061 ttggtattaa atcagcaaag gcagcaaata tctatacggt ggcactaaaa caaccaatgc
     9121 aaatcgatca gagtgaagca gacatacaaa ttgaaagtct acaagaactg gaacaaattg
     9181 ttttagaaaa aggttagtga attaatggaa ataaggctat atatctgtgc tagctatttt
     9241 gactaaccaa aaaaacttgc tataattcag gtgtagtaaa aggtgctggt aacacctttt
     9301 acgaaaatgc ttcggcagtg ataatgggat tggcttttta agttttttat tttgctcgca
     9361 atccctaagg tcatgtgaat gatcctaggg attgctagaa tattttgcaa cattctatct
     9421 atttcagaac gtaaccttcc ccagttcatt gggattcatt agcatacaac caacatttca
     9481 aaaagaaatt gcactacacc attattagct attttgacta accccaaaaa acttgctata
     9541 attcaggtgt aggtaaaagg tgctggtatc accttttacg aaactgcttc ggcagtggta
     9601 atgagtgtgg cttaaaggac tagtccttta agctttttac tttgttcgca atctctaaga
     9661 tcaagttaat gatcgataag attgctagaa tattttgcaa cattctattt cagaacgtaa
     9721 cctttcccat tccactgtga ttcattggca accctcctca caaccaacgt ttcaaaagaa
     9781 attgtaaagt tgttggggta ccacgaccga acaatactac acctgaatga tagtaagttg
     9841 tctcattaag tatagcatat cctctttttg ggtaaataga aatcggttac attttttatc
     9901 caagaccaag ttttttcatt tggtcttatt tgctataccc aaacaattcc gcagtatagt
     9961 cgacggtatt gattttatca ggtgagtaat agaaacttgt tttccttccc caaaacatta
    10021 cctataaagg agaaaaaatg gtatcgtaat tatataggta taggatgaaa tttttttcta
    10081 agaggagggt tgttgatgaa aaatttacag ttaaaattta ttgttgatcc atcgattgta
    10141 cgctggtttc ggattatcaa tcaatttgag cgcaatcaac ttgccacaaa aggagaatta
    10201 gcaaagagta atcatgtatc tgaacgaacc attcaaacag atgtcaataa aatgaaggac
    10261 tacttttctg aaagtgttca atttgtttca acgaaaagtg ggtattcttt tcagaaggaa
    10321 agaccggttc tttttctcaa gcagaaggag caactactgg agaacgaaat tttatttgag
    10381 ttgttaggga atgtctttta tggcgaattg gaagatatga cggagctaat tgatcgtttt
    10441 catttatctg aagcgacttt tcgtcggtat ttgaagcaga ttcaaccagt tttggaagaa
    10501 tacggcttag aattgagtct ttatcctctt gatttaaaag gagaagaggc aaatattcgc
    10561 aagtttttta aagacttcta ttatgagggg gagatgacgc cccatactct tgttccacca
    10621 agggatcttc atgaacttgt gcaagctaca ttctatgata aatttgagta tttctcgatt
    10681 ggaacaggga cttctcctgc tgaattttat tatagtttgt atattgcgat tgagcgtgtg
    10741 agacaaggaa aaacgatagc cattcctcaa ggtctgattg agcgagtaat tgctagtgag
    10801 aattttcgct taatgtattc cttgaaagaa gcgattcaaa caaattatca agttgcttta
    10861 tcagaagagg agttttgttg gttgtattt
//
LOCUS       DACDYJ010000083        10661 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDYJ010000083 DACDYJ010000000
VERSION     DACDYJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.084"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            333..2279
                     /locus_tag="IWM51_002351"
     CDS             333..2279
                     /locus_tag="IWM51_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9971726.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2282..2737
                     /locus_tag="IWM51_002352"
     CDS             2282..2737
                     /locus_tag="IWM51_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9971727.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2751..4079
                     /locus_tag="IWM51_002353"
     CDS             2751..4079
                     /locus_tag="IWM51_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9971728.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4113..4397
                     /locus_tag="IWM51_002354"
     CDS             4113..4397
                     /locus_tag="IWM51_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9971729.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4399..4914
                     /locus_tag="IWM51_002355"
     CDS             4399..4914
                     /locus_tag="IWM51_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAP9971730.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4930..5592
                     /gene="rpe"
                     /locus_tag="IWM51_002356"
     CDS             4930..5592
                     /gene="rpe"
                     /locus_tag="IWM51_002356"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9971731.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5599..6171
                     /locus_tag="IWM51_002357"
     CDS             5599..6171
                     /locus_tag="IWM51_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9971732.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(6358..7878)
                     /locus_tag="IWM51_002358"
     CDS             complement(6358..7878)
                     /locus_tag="IWM51_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP9971733.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(7974..8099)
                     /locus_tag="IWM51_002359"
     CDS             complement(7974..8099)
                     /locus_tag="IWM51_002359"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9971734.1"
                     /translation="MYKRFESGYLQWPRSRKEVLLTGLSIEEKCTLKETKKGAIY"
     gene            complement(8121..8306)
                     /locus_tag="IWM51_002360"
     CDS             complement(8121..8306)
                     /locus_tag="IWM51_002360"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP9971735.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8290..8472)
                     /locus_tag="IWM51_002361"
     CDS             complement(8290..8472)
                     /locus_tag="IWM51_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971736.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            8713..8931
                     /locus_tag="IWM51_002362"
                     /pseudo
     CDS             8713..8931
                     /locus_tag="IWM51_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9426..10025
                     /locus_tag="IWM51_002363"
     CDS             9426..10025
                     /locus_tag="IWM51_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9971737.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            10089..>10661
                     /locus_tag="IWM51_002364"
     CDS             10089..>10661
                     /locus_tag="IWM51_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971738.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         3525 a         1674 c         1990 g         3472 t
ORIGIN      
        1 taggactcaa ttgttcatag atgagcattt gtaattaagt tatattataa taaaaaaata
       61 cgaattttag tgacgccatt atattgtttt tatttttcaa ttgtaatatt tcatattact
      121 tccataataa ggagtatttt ctattggaat atcttagctc ttttaaggat ttctcctttt
      181 ttgtttctca ttttttaaga ttatttggat ttcctttaca acaaacttcc acaagtatta
      241 ttgacataga tgtaattgag ggaagggggt tccagtgcta gaataaataa tgaaaagagg
      301 taacaaatgc tagcgttaag aggtgaacaa gtttgattga aagacatgta aatttattaa
      361 aattattaag tcgacaagaa gactttaaac ccgcttcttt ttttagtgat aaattggcag
      421 tgtcgaccaa gaccatttat caagatattg aattgttgga agattatgta gttaattacc
      481 aagtaacaat tgagcgatta ccttctttgg gtatcaaact cataggtgat aggaaaaacg
      541 ttgaacattt gatttcatcg gtagaaaatt atcttgaaaa agatatttat tctccagaaa
      601 aaagacgtgt cgagatcatt aaagaagttt tgtttggtga taagcctgtt tctttaactc
      661 atttgtctca aagttttatg gttagtaaaa catcgttata caatgacctg agaattatca
      721 ataaaattat tggcagtgga gaagttaagt tgcagtcatc tgcagaagga attagtttta
      781 ctggtaatga acaagcaatt caacgagcaa tcaagcaact tgtattctat tatgcgaatg
      841 acatggaatc aaaaacattt tttcaagttt taaatatgtt gtttgattca aaagttatta
      901 gtgtcattca tgatttgcta ttcaatgatt acagtgaact gactgaaaag gtttctgatt
      961 actatgttcg gtcattgatt tctactttag ttatccaatg taaccgttta ctgaaaggtc
     1021 atcatttaac tgttgaagaa gaattcctct tcaatagcat tcgctatatg gaaacgtaca
     1081 ttgtggcaaa tggtttaatt gaagaattga aggcaaaatt gaatcttcca ttttatgacg
     1141 cagataaaga atatctatgt cgtcaactat ttgcacatcg tgtgacaaat aatttaaaaa
     1201 ccagcaatga agagtacgct atgagcgttc ataaattgat tgaaagaatg agtgagattg
     1261 aaaaaattga tttgactcaa aatgatcgat tatatcaatc cttattgtat catgtgccag
     1321 caatggtcct tcgtttaaaa aaaggtatta aaattcaaaa tccgttatta gaaaatatca
     1381 aatctcaata ttcagaactc tttagcatag tatggtacgc catgtccatc cttgaagaag
     1441 aatttgatgt gactttaacg gacgatgaag tttccttaat tttgattcat tttcagattg
     1501 ctattgaaga tcagtccaaa gcaaataata tcatcattat ttgccaatac ggtatttctt
     1561 ctgcgcaatt aatttacaat aaagttcgac ggtttttacc agcaaaagat aatgtagaaa
     1621 tatcaacttt agataagttt catttagctg aaattgggga agttgatttg attatttcct
     1681 ctctttcctt tgatgcaggt ggtataccct tagtaagggt atctccattg gtaagtaaaa
     1741 cagattatat gaatattatg gaagcttata ctaaatattt aatagaagaa ccttcgcgat
     1801 ttattcaaaa tgaactgatt cctaaagcgg ttaaagttcc caccatatct aaatttgttc
     1861 aatcatcttt agtaaaaaca ggagtacagg tggaaagcaa agaagaatgt ttggatttga
     1921 tgatttccga actagaagcc aattcattag ttactgaaga gtttcgtgct tctatcttct
     1981 ctagagagaa aatggggaat actaatttag aaagtggtgt tgctttaccg catgcgaatc
     2041 catcaatggt cattgaatcc aacattttta ttgtagcatt aaaaaatgcg attaattggg
     2101 gcgatcgaaa agtcaaatta gtcattatgg ttagtttagc tgaagaagat gtatctgaga
     2161 ttagaggagt atatgaagaa ctttaccaat ttattgggca aaaagaaatg gtcgatcgct
     2221 tgttacaagt tcatgatagc gaaaaattac taagaatttt tcaacaaaaa ggagaataaa
     2281 aatgtatcta gataaagaat tatgtttatt tcaattagaa gtgaaagatc aaaaagaatt
     2341 gtttcaagta atgtctgaac aattaaaaaa cgctggttgc gtgaaagata gtttcttaga
     2401 aggtatcacc aaccgtgaac aagaatttcc aactggatta gaagtaaatc aaattgggtt
     2461 tgctatccca catacagatt ccgcacatgt aaatagctct caaatctgtt ttgcctcatt
     2521 aaaggaacca ctggtatttt cagatatgac agataaatct catgaaatac ctgtacgttt
     2581 agtctttatg ttggcaatgt cacaacctca tgaacaaatt gatacactac aaaacttagt
     2641 tagcctattt caaaatgaag aaaaagtaaa tgaattactt gcatgtacca cgaaagaagc
     2701 atttattgag ttagtacatt ctgcaggagt ttattaggag gaatagagag atggatattg
     2761 tattaaatgc ctttgaatgg tttattggat tagggtcaaa cgtcttttta ccactgatta
     2821 tttttgtaat cgggatatta tttggattaa aacctggtaa agcatttgtt tccggcgtta
     2881 ctgttggtat cggttctatt ggtttaggat tagttttaag tttattatca gatggtttag
     2941 gaacagcaat tcaacaaatg ggggaacaat acggtggtgg cctgaatatc ctagatattg
     3001 gcgtaggtgt tggtggacct ttggcttact ctacatcatt gggaatttta atgattccaa
     3061 tttcattaat tcttaacttc atatttgtgg ttttgggttg gacaaaaaca ttgaatgttg
     3121 atatttggaa cttttggttc gcaattttcc taggtatgat tactcaatct gttacgggaa
     3181 acttttggtt tggcttaatt ggcgcgattg tcgcagtaat tttacaatgg ttcttagcag
     3241 atcgtacaca aaaaactgtt agcgaatttt tcggctatcc aggaatctct atcacacata
     3301 tgatgggatt atcaggagta ctatttgcca aaccagtaaa ttgggttttt gatcgtattc
     3361 ctggatttaa taaaatagat gcagatgcag aaacattaac aaaacgtttc ggtgttttag
     3421 gtgacacagt tgttattggc ctaattatcg gtattgtagt gggtattatg gcaggctatg
     3481 acgtagcagg aattggaaac ttaggaatta tgactgcggc agtgatgaag attatgccta
     3541 aaatggtagc aatgtttatg gaaggtttaa tgccaattgc agaagcggcg aaagaattta
     3601 cgaataaaaa gttaaatggt cgccaagtaa acattggaat ggatgcagcg ttaactgtgg
     3661 gtcatccaac tattatgtca acttctctat tgcttgtacc aatctcatta ttgctagcac
     3721 taattcttcc aggaaataaa gtattacctt ttggagattt agcattcttt gctttcgcca
     3781 tctgtttaat gattccttat tttaaaggaa atattgtgag atcattagta ggatgttcgt
     3841 tgtatgttgt tacaacatta tatatggcaa catggttggc accaattatc actgatgtgt
     3901 ttacattagc aagtttcgat gtggggacag caggattagt aactatgttg ttatgtggat
     3961 tatggccagt agggttgttt gttttattag catcttactt tggtgtaatt ggaataggtg
     4021 gattaggagt tgttttagta ggtgcttcta tctacatgaa caaagtgaag aaagcttaat
     4081 cataaaatta aaataagaaa gaagagatca atatgaaaaa attattagtt atttgtggag
     4141 caggtcatgc tacttctaca attgcagtag caaaattatc aaaatggttg gaagaagaag
     4201 gattaacaga tcaagtgaaa atttatcaat ctaaaattgc tgatgagcta aataaaattg
     4261 atgaatatga tgctgtagta agtacaacaa ttgtccctga tgtaattaaa gctaaagtaa
     4321 cgaatggctt gggcttatta acaggtattg gtacagatgc catttatgca gatgtaaaag
     4381 aaagattagg tgtttaatat gcgttttcat catgacactt tgactattga aacagtttca
     4441 gggcgtcctt cgtatcatga tattcgaaaa gaacttttag atacagttga tacagcaggt
     4501 attgagaatg gaatgttagt aatttcgagc ccgcatacaa cttgctcact cttttttgaa
     4561 gaaacgatgc atgacacaaa cttttttggt gatgattatt tacatgtaga catcaacaat
     4621 gtaatggaaa cgattattcc aaaaatgacg agtgaaaatc aatacaacag tccggggcca
     4681 aaacacattg attttggaac aagattatct gatcccaatt accctgcaga aaaatgggtc
     4741 atgctaaata ccgatgcaca tttacgttca tctatttatg gaaccaactc aatgacttta
     4801 attgttcaag aaggtaaact tttattagga gaaatgggcc gtgtctattt tgtcgattgg
     4861 gatcaactaa gagagcgaac tagaacaatc aatctcatga ttatgggaga ataaacgggg
     4921 gaatagagaa tgacaattgc tccatcattg ttaaatgcac ctatcgcaga agttaaagag
     4981 ttatttgaaa tcttaaaaga aaagaatgtt gattatttgc atattgatgt aatggatggc
     5041 tgttttgtac cagatatggc ttttggacca agtgttatta aagatattcg tccactaaca
     5101 gacgttgtgt ttgatgttca tttgatgatt gatacaccag aaaaaattgt cacaagattt
     5161 gtagaagcag gagcagatat tgttaccttc cacattgaag ctaccaaaca cgcaatgaag
     5221 gtcattcagt tagtcaagga agcaggagca aaagtaggga ttacttttaa tcctgcaaca
     5281 ccagtcagtt ttattgaacc agtattatct ttagtcgatc aagtattagt aatgacaatc
     5341 aatccaggaa cagagaataa gcattttatt caagagacag tagttaaaat cgaacagtta
     5401 gatgtaatac gcaaacaaaa tgattatacg tatgatattg aagtagatgg caagattgat
     5461 aatcaaacga ttaaagtttg ttctaaagca ggggcggata tttttgtatc aggcggtttc
     5521 atcttcaatg ggaaaaatac cccgcaagaa caaattgata aactaaatca atcattagag
     5581 gaagtgaagt aaaagatcat gaccaataaa gaccaaaata aaaactattt agaagcgatt
     5641 atcttagaat tacaaaataa ctcaaaacgt attaattctg tagagttaga acaatttagt
     5701 gagaaaattg ttcaagctaa acacatcttt ctagctggtg caggacgctc tggaattgcc
     5761 atgcaagcat ttacgaatcg cttaatgcat ttaggttttt cagtcagcct tgtcggtgaa
     5821 attagttcac cacattcgca acctggagat ttattaatta tctgttctgg ctcaggtgaa
     5881 acaggtagtt taaaaaattt agcggccaaa gcaaatcaaa gtggtgtaag cattgcttta
     5941 gttaccatga aaaaagactc gtcgattggt caattagcag attctgttct tgtattacca
     6001 ggtattacca aagatgaaaa tcaacgagaa gaagatactt tttcacaacc tatgggttca
     6061 agttttgaac aattagcatt tctcaccttt gatagtttag tgttatcttt aatggataaa
     6121 acaggtgaaa gctcagaaac gatgtttgct cgacatgcag attttgagta gtagatttta
     6181 aagataaaaa accattctct aaatgctaat tattcagcaa atatgagaat ggttttttta
     6241 tttgattaga cataacttaa taattcagta gttttgagcg taagcgtgtc atagaatggt
     6301 acctaaacac aaatagcccg atttgataca ctcaaatcag gttatttgtg gcttatatta
     6361 tttacagatt tcttgtatct gctgattcca tggcaaataa gcgcgtagcg cttcaggtgt
     6421 catcgaagac agattgggaa tctctgtaaa aagatgattt aaatatttgc ggatgtttaa
     6481 cccattcgca acagctgtct ggataatact caatataatt ccatttgatc tagcgccttt
     6541 taagctttta gaaaatagcc agttttttcg accgatcact aactctttga tggcacgctc
     6601 tgctcggtta tttgaaagtt ccaagcgtcc atcaagcaac acaatctgaa gtccgtcttt
     6661 atgattgact gcgtattgaa ccgcctttcc aagctgcgat tttggcaaat aagcaaaact
     6721 ctcacaccaa tgaaaaaatt cagtcatcac aggctgcaac gtttccttcc tcgctacaaa
     6781 cctttcttct gcgctaagct tattaaaggt ccgttcgatt cgaaacatct tgtcacagaa
     6841 gtctaatcct attttggcat gactttttgg ggacggttgt ttacccaatg cttcaaaaaa
     6901 ctttctgcga acatgcgccc aacaataaac attgtttacc ggcagctttc cataggccgc
     6961 gtaaccatca gtatgcaaga aaccgctaaa tgattctaaa aaactttttg gctcatcaac
     7021 ggaacgcgaa tttgcgtgct cgtagagcac aacttgtttt tcttcgcttt tacctgaagc
     7081 atataaccag taaaaggtct tttctttatg agtattcaat acccgatagc ttgtctcatc
     7141 cgcgtgtaag acctcctgat tgcttaattc ttgatgaagg caattgtaga ttggctctaa
     7201 aaccatttca cttaccttga tatgccaatt agtgattttt ttacgatcaa gagggaaacc
     7261 aagtgcatgc cattcttttt cttgtcgata tgccggcacc tttttaacaa atttttccca
     7321 aatactctgt gtcacaattg aagaggaacc tagactatta ttgatcaacg gtttaggaac
     7381 aggcgctttt ttgatttttg tttctcctaa tgcttcacat tctctgcaat gataggcatg
     7441 ttggtagatg acttgttttt ctatatgggc aggaacatac aatggctctt ctctaagcct
     7501 tgtcgcaccg atatcatgta aagtatgacc acagtcttca cacaaacaat cggcaccttt
     7561 taattcataa tgaataggaa cttcaggcag accttctaat gaaagtgctt tgagtccctt
     7621 gcgttttctg cgtttaaaag aagataccgt aatgggttcc tggctttctt gtccagtgtg
     7681 ctctggttca ttaaaagttg agtcgtctaa taaatcgatt tgcccatcta cgggtggtgt
     7741 ttcttttttt cgtccaaaca agcgtcgatt catttcagca atttgttctc taagaagagt
     7801 attttcctgt tgctggattt caagcaactg tgttaattgc ttgatttgta caagaagttc
     7861 agattccatt tttgtcattt ttacaccacc attctagcga ttttcttaca tctattatac
     7921 cattttatga caacgaaaaa cagacaaaat ccttgattta acaaggtttt cgtctaatat
     7981 atcgcacctt ttttcgtttc ttttagcgta catttttctt cgattgaaag gccagtcagt
     8041 aagacttctt ttctggaacg cggccattga aggtacccac tttcaaatct tttgtacatt
     8101 aatacaaatc cattttgatc ctagtaaaga gccttgaagc gatctttctt cgtaccgcaa
     8161 aataagaaaa gcgcttgatc gtaaacatca aggtcatatt cttctataac gaaagctgct
     8221 agtccatcaa ttccttttcg aagatctgtt tttccacaaa cgataaaaat atgtttcaat
     8281 gcagttgcat caatgagcat tggcattcaa ctcttttaac aaggtataca aaagatattt
     8341 atccattcca ttataaataa atatttcctt atcttccgat ttgattctgc aagctagttt
     8401 tggcttggtg tcaatttgcg atttttgagc cgcacttgaa aattgaactg gtacaatatc
     8461 ttttttattc attcgttttt ctccttctat atttgatagt ttaattatag aagaaagata
     8521 atcggcgtac gagatactat cctatgacac gcttacattt aatttaatat tttgcctcaa
     8581 attagccaca ctttttatag aactaagagg aaaaatgaag ttttcttcct attaatatag
     8641 ttgtttgaaa tcttaaatat agcgttttac gatgtaatgg gaagtatggt gaataaatca
     8701 actatgttcg tacgaagcat ttactcaact aatttaatcg aatctttcaa taaacaaatc
     8761 aagaaataca gccaccgcaa ggaacagttc caaaatgaag agtcgatgga acgtttctta
     8821 gtctcgtctt ttgatactta caaccaaaaa ttcctaggtc gcagtcataa aggtttccaa
     8881 caggccgaag gcgagctcga acaaatgctg agtcaaccga tggagaatta gaaacaatct
     8941 acagtaggga agaaccattg acacaaaatt attgacgctc ccaactaaaa gtgtcaaaat
     9001 aatgatgaaa tcatttagtt cttataaatt agaaaagaga aaataaatat tccatatcta
     9061 ctttattgga tccgtaacat gactaaattc tgaatacttt ttaaagctta tcatttggta
     9121 aaactgaata taagttaatt atatagactg aaaattttga attttcagaa tagaataact
     9181 taaaagtact gacaaaattt tttaaagggg caatattaat gttctgagat aatcaaatcc
     9241 taccgaaaat atcaaaaatc caatgtgaca caatcagatt gtgtctcatt cgagggaatc
     9301 acacaatccc acgtggctct aggcagaatt gaatttttgc ttggggcttt ttccttatat
     9361 tgattttgaa tgagacttat tatatgttat aatgcaatta agtatcattg gagagggcga
     9421 agaaaatgaa gacggtagaa cctattcgag acaaaaaaaa gatcgatgcg atgaaggcaa
     9481 tcttggcttc gggaaaatat ggccaacgaa atttggttct tttttcgatt gggatcaaca
     9541 ccgcctatcg catttctgat ttgcgtcaat tgaagctggc cgacgtgctg gaaatttcac
     9601 gcggtcgcgt ggtggtcaaa gatcgattag cgatgaagga acaaaaaaca gctaaacata
     9661 attcggtcta tatttccaac aaattgcgaa aaacgatttt ggagtacgtt caacaggaat
     9721 ttcctgatca actccttcag caagattttg acggttattt gttccctagt cgaaaaggga
     9781 cgaatgaacc gctcaaccgc caaagtctgt ggcgaatcat tcatgatgct ggcacggcaa
     9841 tcggattgaa agacattggc ccacattcca tgcgaaaaac gttcggttac tttctctata
     9901 aacaggggac caaaacagaa atcattcagt ccttgttgaa ccattcttct cagcgcgaaa
     9961 cattgcggta tattggtatt acacaagaag acaaagatac agctgtaaaa agcttagatt
    10021 tataatataa aagttaagtt taaacatgga tagttttaaa ttagtagatt gagtgttggg
    10081 aggaaagaat gagtacatta tatattatag gaaacggttt tgatttaagt catggtttaa
    10141 aaactagata cactgatttt tcaaaatggg ttgaaaaaag tcatagtgaa atattcgata
    10201 aaatagacac aatgataaat gaaaataatg aggtgagaag tacagctgat gataagatag
    10261 gttggtttaa ttttgaaagt agtttgcatg agttaattca aagtccagtc tttgaagatt
    10321 atgtagaaga gtctaaggat tatctcatag gttatggaca tgaagattgg tcagattctg
    10381 cccatcatga ttttcaaaat atggtttatg attatgttgt ttctctagaa gaattacaac
    10441 aacttttcaa ggaatggata cgcaaagaaa tacagccgac aatagaacat aaaattccaa
    10501 aataccaatt ttcgtccgat gtactattct ttacattcaa ttatacagat gttttggaaa
    10561 ttctatacaa gcaaccgaaa gaaaacatat atcatattca tggcagttat gacgatataa
    10621 taatcggcca ctccgagaat tttgacaact ttataggaga t
//
LOCUS       DACDYJ010000084        10432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDYJ010000084 DACDYJ010000000
VERSION     DACDYJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.085"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(142..894)
                     /gene="xth"
                     /locus_tag="IWM51_002365"
     CDS             complement(142..894)
                     /gene="xth"
                     /locus_tag="IWM51_002365"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP9971739.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1007..1927
                     /locus_tag="IWM51_002366"
     CDS             1007..1927
                     /locus_tag="IWM51_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9971740.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2000..2935
                     /gene="murB"
                     /locus_tag="IWM51_002367"
     CDS             2000..2935
                     /gene="murB"
                     /locus_tag="IWM51_002367"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP9971741.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3233..4711
                     /locus_tag="IWM51_002368"
     CDS             3233..4711
                     /locus_tag="IWM51_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9971742.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            4988..7048
                     /locus_tag="IWM51_002369"
     CDS             4988..7048
                     /locus_tag="IWM51_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971743.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7131..7700
                     /locus_tag="IWM51_002370"
     CDS             7131..7700
                     /locus_tag="IWM51_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9971744.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7875..8681)
                     /locus_tag="IWM51_002371"
     CDS             complement(7875..8681)
                     /locus_tag="IWM51_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971745.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8819..10333
                     /locus_tag="IWM51_002372"
     CDS             8819..10333
                     /locus_tag="IWM51_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP9971746.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3703 a         1739 c         2054 g         2936 t
ORIGIN      
        1 acagcttttt cacaatctct cgataaacag tgttcaaaag ccagtttctc ggtattatgc
       61 caaatttatg caaaatgtgg aaagccattt tcacaaaatt tttcttaata ctcgaaacta
      121 aacgactttt tcacaacctc tttatttcaa atctaattct actggacaat gatcactgcc
      181 cataatgtcc gtatggatct ttgcatcagc tagtcctggc tctaaatctt tgctcgtcaa
      241 aaagtaatcg atccgccaac cagcattgtt tttccttgaa ttgaatcggt agttccacca
      301 agagtagaca ccttcctggg ttggataaaa ataacggaat gtatcgataa acccgttatc
      361 caacagtcga gaaagagccg ctctttcttc tggagtgaat ccagcatttt tttgattcgt
      421 tttccagttt ttcaaatcaa tcttttgatg agcgacattt agatccccac acacgatgac
      481 tggtttttgt ttttttagat tttccaaata attataaaag gcttcttccc attctaatcg
      541 gtaatctaaa cgttttaatt cgctttgaga atttggcgtg taacaggtta caagaaagaa
      601 atcggaatat tccaaagtaa tcagacggcc ttctgtatca tggatatcga tacccattcc
      661 gtaagtggca ttcaatgctg gttttttagc aaaaatcgct gtgccggaat agccttttcg
      721 ttccgcgtaa ttccaatatt gataataacc tggaagatct aaatcaattt gtcctgcttg
      781 gagtttcgtt tcctgcagac agaaaaaatc ggcgtctaac tcatgaaaaa cttctaaaaa
      841 atttttattt acgatggcac gaagcccgtt tacgttccaa gagataaatt tcaataaaag
      901 cacactcctt cgaattaatt tccttacctt tgcgatacta ttgtacctca ttttagtaaa
      961 aaatggtgta agatgaaagt aactaaatga tccaggagga aagtagatga agattggtgt
     1021 aatcggtgta gggaatatcg cagaaaaagc atatttacca acttatgcga aaaaacaagg
     1081 aacacttgat ttttattttg caacaagaaa taaagaaaca aaagaaagaa ttcaaaaaga
     1141 gtacgggttc caacatctat atgaaacgat cgatgagctt ttagaagaaa aaatcgaagc
     1201 atgtatgatc cacgcagcaa caaaagtaca ttatgaatta gccaaaaaat gtctagaaaa
     1261 tggtatccat gtttatattg acaaaccact gagtgtccat ctaaatgaag ttcgtgaatt
     1321 gcaagaatta gctgataaga accaaataat tttgatgatc ggtttcaacc ggcgctttgc
     1381 tcctatggta gaagaattga aacgtatccc agataagcgg ttgatccacc tacaaaaaaa
     1441 tcgaatagcg gcaaaagaaa cgacagagtt cgtcctttat gatttgtttt tacatcttat
     1501 tgatacagct gtgtatttat tagatgaacc cattttgaga acaaaatacc agataagaga
     1561 aacaaaggaa tttttggaat atgccatttt acaattagaa acacaaaatc aaacggctat
     1621 tttaacgatg gatttagcaa gtggcgctaa tatagaaaaa tatcaagtaa caagtaaaaa
     1681 tggtacttat gaagtaaacg atctgaccca gcttgtcatt caagatgctg atggaaaaaa
     1741 agtgaaagaa tttggcgact ggacaaacac gctagtcaaa cgcgggtttg agccaatggt
     1801 agaggccttc ttttctcaga tccgtttagg taaatcggaa acagaattgc ttaaacaaaa
     1861 agaagttgta agaagtcatg aattatgtga ggaagtgctt aaagatcact accgtcatca
     1921 actttgaaca agactggcaa gattgtatta agcgtgatag aataatgagg acgtttgaga
     1981 aaagaaagga aaatcctcag tgaataaaga agaaatagtt gaaagcttag ccacgattac
     2041 tatattgttt gatgaaccat tgatgaacta tacgtttacg aaaacaggtg gaccagctga
     2101 tgtccttgca ttcccgaaaa aacaagaaga agtaaagcaa atcatcgatt attgccgaat
     2161 ccatgatata ccttggatgg tattaggaaa tgcaagcaat ctgatcgttc aagatggcgg
     2221 gattcgcggt gtagtcatca tgctgactga aatgaaacag attcatgtca aaggaacgat
     2281 ggtcattgcg gaagcaggtg cctcactgat tgatacgact tatgcagctt tggcagaatc
     2341 attgactgga tttgaatttg cttgcggcat cccgggaagt gtcggtggag cggtttatat
     2401 gaatgcaggt gcgtatggcg gagagatcaa agatgttttt gctgaagtgg atcttttgct
     2461 tgaagatgga acattgaaaa ctttgacaaa agacgaaatg gtattttctt atcgtcacag
     2521 taaagtccaa gaattacgtg ctatcgtatt ggaagctcga ttcagtttgc aaacagggga
     2581 ttatgaagca atcaaagcaa gaatggatga gttgaccgaa ttgcgtcaat caaaacagcc
     2641 attagaatac ccttcatgcg gaagtgtctt taagcgccca gtaggacact atacaggtca
     2701 gctgatccaa caagcaggat tgcaaggcct gaaatggggc ggtgcacaag tctctgaaaa
     2761 acatgctgga ttcatcgtca atatcgatca tgcgacagcg acagattata ttgaattgat
     2821 tgcgcacatc caagaagtaa tcaaagaaaa atttgatgtg tctttagaaa cagaagtgcg
     2881 gatcattgga gaaaaacaag caatatcaga aacaagcaga agtttagcca attaataagt
     2941 aatggaactt aaaataagta aaagagcagg acggtggccg tcctgctctt ttttgtggtt
     3001 gtttacgcaa atggcgcaaa tcttgtacat tttaacacaa taaaacaaaa aatatagaat
     3061 atttcctcat aaatttttcc attttttgca atattctcaa aaaaaagata aaaaatgaga
     3121 ataactgatg aaaaaacaag cataatccat cataaaatca gtataggtga aaagatgaac
     3181 tttcatctgc atatcaagta attggtatga ggcatttttt gaggtgtagg atatgaagtt
     3241 ggattacctt atagaagagg aagatagaga aaaactgctg ctagctagat atttagaatt
     3301 tgagaaaaag aattatgtag tgctgcctaa gttatatgaa acattaggtt tgtctaaatt
     3361 taaaacaaga aattatatca tagaattaaa taaagaattt actgaattca aaacaaagcc
     3421 agagatccaa atttcagaag aagcagagat ccatgtcgaa cgtctgaatt tgacggatat
     3481 caaaaattta agaagaatct actttcaacg atcaaaaatt gctcgactgt taacggagat
     3541 cgtggattca aaagtaacga tcgaaaaatt tgcagagaat cattttttaa gcatatcaag
     3601 agcctatgta aaaagaagag aattagtctc ttttcttgaa cagcatcaaa taaagttaaa
     3661 gaaaaacata ttagttgggg aagaaataaa catacggaac gtattatttg gggtatattt
     3721 cggggtcttt aatggttttt ctatgccatt tagagaaagc gtcatgaatg aagcaaagag
     3781 tatcgtgcaa tattttgaat atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa
     3841 attcaatttg ctaaccacta ttagtttgtt gagggcctat gggaatcaac caatcggaaa
     3901 acgtttcttt tcagatgaat actttaagag ctcagaagga atgaagatca tcaaggattt
     3961 atcacatatt tcttttcttc atatccctaa agagcagatc attgatgaga tttcttatat
     4021 catgttgttt ttgatcacgg aagaagaatt tgaattagat ggcgcatttg gacttgcgga
     4081 agctcatttt catgatatcc acatgattag tcaaaaaatg tcagtggaat tgattgatca
     4141 attaccattt tcgattgaaa tctctgaaga agaaagacta tatatacgaa aagaacttcg
     4201 tatgggtatg atgcatgtca atcgaaaaaa ttggatattc gactttgaga tgactagctt
     4261 tacgtctaaa aagcagctac agtttttctt cgagacgtat ccaatattca gtacagcaat
     4321 ccaaaaggtc atacaagaga ataaacgcta tttcttggga aaagatgaac gtttcatgat
     4381 tcgcttattt tatgattatc tctttttatt ggtcggtatt tgtccggtat catattttga
     4441 agcaccgatt catgtctgta ttgatttttc gcaaggatct gcttataccg attacattat
     4501 ttctcaagtg aaaggattta aaaattacaa tattgtgatt gagagtcgaa tcacttctcg
     4561 tacgaatatt tatttatctg attgtttact tgaaaaattt aataaagaac agataatttg
     4621 gaaaaatcca ccgactccag atgactggga atattttgga aataccatca tcagagaaaa
     4681 aaatagacat ttaactgaaa aaatcagcta gtatttatgg atggcagctt tgtctcacat
     4741 atttattagc tgatttatgt ttttttcaaa gaaaaggtaa ctagatataa ctagatataa
     4801 ctagatatat tacttaacaa aattaaaaag tacataagtt attattccga ttttcttgaa
     4861 acaataaaaa atcaaaaaaa atactctcct aatagtccat cttcttttga ttttatttat
     4921 ataatgaaat catcttatga aaattactac ttcatgttta gacaagagta gggaggacta
     4981 agaataaatg tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt gtcgtttaga
     5041 taaggaacgg tcgtgttctg aaaaacacta cgttaagttt atgatgcgca cgatgctgat
     5101 cagtttatct gtaggtgtct atatgcatgt acccgttcag gcaacgacac aaaccatcaa
     5161 ggtcgtacgt acctctgttt ctgagaccac tgattcccct atcactatta ctcatacttt
     5221 cactgatgca aatggaaaac agctacttcc tgcattgact tcctataata aagtcagtta
     5281 tcctccaacg ataaaccact actggttaat tcctaacaaa acattatgga catcggaaac
     5341 cggaagcagt gtgccgcttt ctatcttttc cgatatgact gatcctacaa aaggtgatca
     5401 gttaaaacag atcactgata ttttgaatca tgctttgaca gatataaagg acggtggaag
     5461 cgcacaagtt tcttatgtat atgaaaaaga ttattcacag tttgaaagaa agaaaatcga
     5521 tgttctggca gggacaaaag tttcgatatc tgatctggta gaaaggctga agaacgcaga
     5581 tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg tggatacgac
     5641 taatacagga acatctacta tttccttgtc ctattttgat ccaattgctt taaaaacaat
     5701 gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct
     5761 tcacttaaga acaggagaga gttacgcccc taaagatctt gttgaatatg taacaaaaag
     5821 cgatggaact tcaggaaata aagaagaaat caaaatgaac gggacagtag atactacaac
     5881 tccgggagaa tatcccgttg tattaactta tatagactcg aaaacatcaa atgtggcaga
     5941 aacaacagct gtagtttatg tagaagcatc tgctgcttca tcgactgtca tcactcattt
     6001 tatagatagt caaacaaata aagaacttca agcagaaaca catatgagta gtgtaaatcg
     6061 tattcctgat ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata tagatttatc
     6121 gacttatgaa caaaatggga aagtcacttc tatttctgat tggatgcaag caaacggatt
     6181 agacagaaaa agtcactggt cggagatcct tgaagtactt cactcagaac tgatgaataa
     6241 taggaagcaa tcaggagggg aaattacctt tacttatgtt tatataccag acaatcgttc
     6301 attagaaggt catgatctta ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat
     6361 ccaatcagca acaaattcct taggtaaacc cgtaccgtta aaggatgtat cggttacaat
     6421 caatgaagta ccattggagg atggttattt gaccaaacaa acaggtgatt ttacgattat
     6481 gtatagttat catgacccgt attcactcca agaactccat gctcagtcac tattacatgt
     6541 tttaccatta aacaaacaga tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc
     6601 agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag
     6661 aagcctagag ttagcaagaa tatctagaaa aatctcagaa tctaataaaa cgataaggca
     6721 agaagctcat ttggccccac aagtttctgg attggaaaat gaaattttag agcctaaaat
     6781 aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg gaagatcaga aggaatcaga
     6841 tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt gattccaccg atgagcatct
     6901 gcagccgcaa gtaccacttc ctagtccggg aacacccgca cctggtggag gtattccttc
     6961 ttctgcttta ggagatttca tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa
     7021 tgaatttgac gcagatgact ggttctgatt tatatctcta tctgttatag tcaaataagt
     7081 agctgagtca tatgttattt ggcataatgg agacgaggag ggatagaaaa atgttagata
     7141 aaacgttacc ttacgcagaa atctggatga caagaccact tcaacaaaat gttccaatca
     7201 cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa tctgcttggg
     7261 cggagattga gacttccgtt ggggaatttc aaacgacaga tgaagcactt gagtattttc
     7321 aacaaaactt tgctccttat cctgaagaac ttgaaagaag gatgtttttt gtagagacaa
     7381 aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt
     7441 atccggtttt tcattggtta gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag
     7501 cattgactgc cagagtcttg caagaatttc ctaccctcca tacggacgga ccaatctatc
     7561 ttcacacaca gacatggagc catcaggcga ttgctcttta taaaaaaatg ggttttactt
     7621 ttattgctga aaatctagac ggcacaccta atccagatta tgaaaaagtt atgcaggttt
     7681 taagtaaatt agataaataa cgaaatggtg aaataagtcc tcagtattgg gataaaaaac
     7741 gaaatagtca ttcctcatat cttacttttt tgagttaata ctatcggata actaatggca
     7801 tgccatacat tcaaaaaaga aggggcagtg acaaaagtat tgtcactgcc ccttctcttg
     7861 ttttttagca tgttttatcg tttgattttt ttaggaataa aattcttgat gaacgtaaat
     7921 ttttggtaat cgtttcgact atagaggtaa ccaaatacac tgaaaacaac tgctccaata
     7981 aaattaacaa ataaatcctt catagtatcc atgattccca cgtctaagta accattttgg
     8041 atgattgttt tatctgtttt accatctgca ttttgacttt caatgattgt ccgagtgata
     8101 tctggaacat gatagacagt gtttccgtct ttacttaact ccacactatt gatttgctgg
     8161 atgatcgtat ctttttgcat atcgaattgc agtaatttat ctgctgaaaa ttccacaaac
     8221 tcccacataa caccaactgt catcgaaaaa cagaaggaaa caactgctag taataaagga
     8281 gtcaagttca tggtttttgt atttttattg agcaaataaa caagggaaaa gccgacaccc
     8341 ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc acattgaaaa atagccataa
     8401 aaatcactta actctcctaa aactgttgaa ctaaaaataa aagataacaa aatgatttct
     8461 agcatatttg gaatctgtat ctttaatatc ttttccagaa aataagggat ttcaaataaa
     8521 attaatgtca gtaataaaag cattgtattt ccccagtgtt ggttccagac ctgatccact
     8581 agcataataa cgacgatgat acgtaataaa atatagattc cgtttgtgat tctacctgca
     8641 gtttttgtac tgttcgtaaa aaaggttttc aaatgattca taaaataact ccttcatgta
     8701 tatccttaat tatactaaga agcgtgtgtt taccaaagct atatgactta aagaaaaaca
     8761 gagttgatat tgtgaaaaaa atcacttaat atagtataag gagaaaaggg ggatacaaat
     8821 gaaaaaatta gttgctggtt tattaacagc cgttgcactt cttgctatga gcgcatgcgg
     8881 cgcagatcag aaaaatacag cgcagacaag ttctagtacg aaggaatctg ccgaagtatc
     8941 atcaggcgct tctgaacaag tatatacaga tccaaaagaa atgaaagaat cttatgatgt
     9001 cgtgattatt ggagctggtg gtgcagggat ggcagcagct atcgaagcaa aagatgctgg
     9061 gttgaatcca gtaattttgg aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc
     9121 aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa
     9181 tgatgccttc tatgaagaaa cgctaaaagg tggtggagga acgaatgata aagaaatgct
     9241 tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa
     9301 actggacaac ttaacgatca ctggagggat gagtgaaaag aggacacatc gtccgtctga
     9361 tggctcggct attggtggat acttagtgga ggggttattg aataacgtcc atgagcggga
     9421 aatccctatt tttgtcaacg cagatgtgac agacattaag aaaaatgggg aaacggtcaa
     9481 tgaagtgact gtacatgttc aaggagaaga accaaaagag gtcaccggaa aagcaatcgt
     9541 agttaccaca ggcggttttg gtgccagcaa agaatttatt gaaaaatatc gtcccgattt
     9601 agctgattat attacgacaa atcaagaagg atcaactgga gatggtatca ccatgattga
     9661 aaaattaggt ggacaaaccg ttgatatgga taaaatacaa attcatccaa ctgtgcagca
     9721 ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca
     9781 agaggggaaa agatttgtca atgaaatgga tacaagggat aaagtatctg cagcaattac
     9841 agcattgcct gaaaaatcag cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac
     9901 agctattgaa ttttatgacc aaaaaggata tgtcacaact ggaaagacaa tggaagaatt
     9961 agcagaaaaa atcgatttac caaaagaaac actagcaaaa acagttgatg gatggaatac
    10021 ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc acagccatgg aacatgactt
    10081 atcgtctccg aattattatg cgatcaaaat cgcaccagga atccattaca cgatgggcgg
    10141 tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa aaccagccaa ttagggggtt
    10201 atatgctgct ggtgaagtca caggtggctt acatggaaac aaccggatcg gtggaaattc
    10261 agttgcggag atcattattt ttgggcgaca agcgggcata caagcagctc gatttgcttc
    10321 ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta
    10381 ataccgaggg tcagctgatt gaacactgtt tagtgaagag gttgtgaaaa ag
//
LOCUS       DACDYJ010000085        10245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDYJ010000085 DACDYJ010000000
VERSION     DACDYJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.086"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            162..392
                     /locus_tag="IWM51_002373"
     CDS             162..392
                     /locus_tag="IWM51_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP9971747.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="IWM51_002374"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="IWM51_002374"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9971748.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="IWM51_002375"
     CDS             1803..1979
                     /locus_tag="IWM51_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9971749.1"
                     /translation="MSNQRYIILTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="IWM51_002376"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="IWM51_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP9971750.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2594..3061
                     /locus_tag="IWM51_002377"
     CDS             2594..3061
                     /locus_tag="IWM51_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9971751.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3146..4030
                     /locus_tag="IWM51_002378"
     CDS             3146..4030
                     /locus_tag="IWM51_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9971752.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4147..4380
                     /locus_tag="IWM51_002379"
     CDS             4147..4380
                     /locus_tag="IWM51_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP9971753.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4468..4548
                     /locus_tag="IWM51_002380"
     tRNA            4468..4548
                     /locus_tag="IWM51_002380"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4502..4504,aa:Tyr,seq:gta)
     gene            4555..4626
                     /locus_tag="IWM51_002381"
     tRNA            4555..4626
                     /locus_tag="IWM51_002381"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4587..4589,aa:Gln,seq:ttg)
     gene            5141..5848
                     /locus_tag="IWM51_002382"
     CDS             5141..5848
                     /locus_tag="IWM51_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9971754.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5908..6552)
                     /locus_tag="IWM51_002383"
     CDS             complement(5908..6552)
                     /locus_tag="IWM51_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP9971755.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6679..6966
                     /locus_tag="IWM51_002384"
     CDS             6679..6966
                     /locus_tag="IWM51_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971756.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7074..7394
                     /locus_tag="IWM51_002385"
     CDS             7074..7394
                     /locus_tag="IWM51_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9971757.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7475..8788
                     /locus_tag="IWM51_002386"
     CDS             7475..8788
                     /locus_tag="IWM51_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9971758.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8785..9504
                     /locus_tag="IWM51_002387"
     CDS             8785..9504
                     /locus_tag="IWM51_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP9971759.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9632..10183
                     /gene="raiA"
                     /locus_tag="IWM51_002388"
     CDS             9632..10183
                     /gene="raiA"
                     /locus_tag="IWM51_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP9971760.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3415 a         1693 c         2187 g         2950 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcatattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaacaatt tggaaaatag cttcttatat tttgcaaaat tgtgagttat
     2461 tgccgaagtg ttggattttg aacaccgtca aaaaaaggtt gtgaaaaata cgggtagcac
     2521 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2581 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2641 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2701 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2761 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2821 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2881 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2941 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     3001 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     3061 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3121 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3181 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3241 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3301 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3361 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3421 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3481 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3541 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3601 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3661 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3721 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3781 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3841 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3901 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3961 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     4021 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     4081 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4141 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4201 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4261 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4321 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4381 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4441 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4501 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4561 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4621 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4681 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4741 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4801 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4861 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4921 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4981 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     5041 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     5101 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5161 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5221 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5281 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5341 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5401 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5461 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5521 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5581 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5641 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5701 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5761 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5821 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5881 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5941 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     6001 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     6061 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6121 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6181 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6241 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6301 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6361 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6421 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6481 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6541 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6601 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6661 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6721 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6781 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6841 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6901 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6961 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     7021 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     7081 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7141 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7201 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7261 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7321 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7381 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7441 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7501 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7561 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7621 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7681 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7741 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7801 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7861 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7921 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7981 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     8041 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     8101 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8161 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8221 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8281 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8341 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8401 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8461 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8521 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8581 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8641 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8701 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8761 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8821 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8881 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8941 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     9001 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     9061 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9121 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9181 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9241 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9301 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9361 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9421 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9481 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9541 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9601 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9661 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9721 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9781 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9841 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9901 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9961 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
    10021 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
    10081 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10141 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10201 acaacgaaaa cttgcagcag ctactgacag ctgccgcaag ttttt
//
LOCUS       DACDYJ010000086         9907 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDYJ010000086 DACDYJ010000000
VERSION     DACDYJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.087"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            4..360
                     /locus_tag="IWM51_002389"
     CDS             4..360
                     /locus_tag="IWM51_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971761.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            376..726
                     /locus_tag="IWM51_002390"
     CDS             376..726
                     /locus_tag="IWM51_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAP9971762.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            755..1534
                     /locus_tag="IWM51_002391"
     CDS             755..1534
                     /locus_tag="IWM51_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAP9971763.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1545..2222
                     /locus_tag="IWM51_002392"
     CDS             1545..2222
                     /locus_tag="IWM51_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAP9971764.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2241..3347
                     /locus_tag="IWM51_002393"
     CDS             2241..3347
                     /locus_tag="IWM51_002393"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAP9971765.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3337..4428
                     /locus_tag="IWM51_002394"
     CDS             3337..4428
                     /locus_tag="IWM51_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAP9971766.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4441..5181
                     /locus_tag="IWM51_002395"
     CDS             4441..5181
                     /locus_tag="IWM51_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAP9971767.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5469..7409
                     /locus_tag="IWM51_002396"
     CDS             5469..7409
                     /locus_tag="IWM51_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9971768.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7496..9418
                     /locus_tag="IWM51_002397"
     CDS             7496..9418
                     /locus_tag="IWM51_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP9971769.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(9698..>9907)
                     /locus_tag="IWM51_002398"
     CDS             complement(9698..>9907)
                     /locus_tag="IWM51_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP9971770.1"
                     /translation="TQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAVGSEEEI
                     LTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         3228 a         1644 c         2204 g         2831 t
ORIGIN      
        1 atcatgatta aagtagttat tgcagaccgt atgggtaaag gacaaaatgt ggccaaaggg
       61 gtagaagcag ctggtggaaa agcagttgtc gtaccaggaa tgggggcaga tatgcgtttg
      121 ggcgatgtga tgcaacaaga gcatgcagat atgggtatct ctttttgtgg tagtggtggt
      181 gcaggtgcat taacagcagc aacaaaatat ggctatccag aacgacatgg tatgcgttcg
      241 attgaagaag ggatcacagc tatcaaggat ggcaaaactg ttctaggttt cggctttatg
      301 gatcaagagg aattagggaa acgtctgaca gaagaattta tcaaaaagca tggaaaatag
      361 aaagagggac atgaaatgag cgttcaaaag acaattcagc aaaaagttat cgtctccggt
      421 aaaggggaaa caaagcaaca tgcttttgca tcagcgttag cagaaattca aaacaaaata
      481 atggaacaat caaacgtatt attgcgaatc gaaccagtca atgtggatat tttaaaggcg
      541 gtcgaaaaca aatacacaga acgctttcta tttttcttct tcccacggca acgtattagc
      601 tatgaagtcg aactctctgt tactgtggct gtaacagaaa taaaattggc agacatatcg
      661 ttcagccagc aaatgactga agatccaaat gggattgcca ttccatttgt aactaaaaaa
      721 atctaatttt gaatcataag ggggaaagaa taacgtggat ttcgtaatca ttctagtcaa
      781 atcggtcatt atcgggggat tacttggttt tgcagcatct gtcggggcag ctcgaatgtt
      841 tcatgcacct aatacacaag gtttaggagc ttttagaaca ttaggcgaaa tgaatgcctg
      901 catgggtgat ccagcttctc atttttcatt tggtttaggc tttttcttta atgcatgggc
      961 ttcttcagtt ggtgctggtg cattcacaca agatgtgacg catcgaatca ttcccaattg
     1021 ggcagcggca gcacttctag caaaaaacaa agatgtcacc cagaccatgc atgatccgaa
     1081 aaaaatgggg atcattggag cggttatcgg ggcagtggtg gtagccttct taaacacaac
     1141 agctgcagca attccagagg cgttacaagt gacggcggtt tccgttttgg taccagcagc
     1201 gacgattctg atcaatacag taatgccagt tattttttgg ttggcagctt tagacgcagg
     1261 gaaacgaact ggtttttggg gcactctttt tggtggtatc gcgcaattga ttatgggaaa
     1321 tgcggtacct ggtgttgttc taggaatctt gattggtaaa ggcgtcgatg agagcggttg
     1381 gaatcgcgtc acgaagagca tgatgatcgc tattgtttta ttatttattt tcagtggatt
     1441 tttccgtgga tttgatatgg aaatgatcaa gagcttccag cttcaagtgc ctaattggct
     1501 tgataacttg cacaacatct ttagtgtgaa ataggaggag aatcatggag acaacagaag
     1561 aacaagcaaa agtattagaa gcaaagcggc aaaagagctt ttggtttgca gactggtctt
     1621 ttccgctaat cgtaggaatt atgtccgctg ctgtatttgc aggaacacac atgtatgtga
     1681 tgtatggtgt gggtgcattc aatgaagttt cgatcgtggc gatgttaaaa gccgggctgg
     1741 atggcggatc ctatggcgca gcggcagcgt ttggtgctag tttcttattt gccagaattt
     1801 tagaaggctc tttagttggg atcttggact taggcggttc gatcctaact gggatcggaa
     1861 ttggggtacc agcaattctc ttaagtatga aaatagcggc accaatcgat aattttgcac
     1921 tctctttatt gactggtttg gttctaggtt tgattgttgg tggcatcatt gtcttgattc
     1981 gtaaatttac gatcaatcaa tcaaattcca cttttggtgc tgacgtcatg atgggggcag
     2041 gaaattcatc tggacgtttt ttgggtccat taattatatt aagcgcgtgt gcagcatcta
     2101 ttccaatcgg cattggcgct acattaggag cactaggatt ctatgcttgg aaaaaaccaa
     2161 ttgctggagg tgctatttta ggagcaatga ttttaggagc aatcttcccg gcagtaaatt
     2221 aatagaaaga gggtcattaa atgtttgata ctttgattaa aaatggaagg ttagttgatg
     2281 gatctaaaat agaggtcgct atagaaaaag gcatgattaa agcagttgca gcagaaataa
     2341 atcagccggc gcaagaagta gttgacttgg agggaaagta ttatttatcc gctgggtgga
     2401 tcgatgatca tgtccactgc tatgaaaaga tgaatcttta ttatgactat cctgatcaga
     2461 taggggtcga aaaaggtgtg acgacggtca ttgatgctgg aactacggga gcagaaaaca
     2521 tccgtgaatt ttatcaattg acgaaatcgg tcaagacaaa tgtgtatgcg ctaatgaata
     2581 tctctaaatg gggcattgtc aaacaagatg aacttgctga tctaacaaag attcaagaag
     2641 atctagtaag tactgcgtta gcagaactgc cggaatttat tgtcgggatt aaagcgagaa
     2701 tgagtaagac agttgttggt gaaaatggca ttaagccatt agaattagct aaaaaaatcc
     2761 agacagaaaa caatgatctg ccattgatgg ttcatatcgg ttctgcacct cctgagttag
     2821 aagaagtatt gtcaagaatg gaaagaggcg atgtattgac ccattgtttt aatggaaaac
     2881 caaacggtat tttagatcca aagacagatc aaattaaaaa ctttgtctgg gatgcttaca
     2941 ataatggtgt cgtctttgat attggacatg ggacggatag ctttaatttc catgtggcag
     3001 aaaccgcact aaaagcagga atgaaagcta cctctattag tacggatatc tacatccgca
     3061 atcgtacaaa tggcccagtt tttgatctag caactacttt agaaaaattg agggtagtcg
     3121 gctattcttg ggaagaaatc attgaaaaag ttacagcagt tcctgcacgg aattttcatt
     3181 tgaagaataa gggccagtta gctgtcaatt atgatgcaga tttgacgata tttgagattg
     3241 ttgaaaatga aaaggaattg aaagattcca acggatttac acgtatagct aaagaacaaa
     3301 ttttaccgat taaaacaatt attggaggcg tcatatatga caattagcta tggaaagttt
     3361 gatttaaaag aagtcattaa tgcatcggga aaaatgacga tcctaggggt ttctaaggta
     3421 tctgaaagtg tcctagcagc acaacgattt ggaggcgagc atttttttga gatgagtgat
     3481 cttgccatca aaaccggcgc ttatctttct caattactga aagtggaaga tgcgcaaatc
     3541 gtttcttctg cttcagcagg gattgctcaa tctgttgctg ctttaatcgg acagggcgac
     3601 atgtaccatg tctatcatcc ctatacagaa aaaatcgcta gacgggaaat catcatacct
     3661 aagggacata atgtagatta tgggacaccg gttgaagtaa tggttgctca aggtggagga
     3721 aaagtcatag aagctggcta tgcaaatatg tgtacaccgg aacatgtttc catgatgatt
     3781 acggaaaaaa ctgctgcgct tctatatatt aaaagtcatc atgcagttca gaaaagtatg
     3841 ctgacggtgg cagaaatggt agaagttgcc aaagctcatc gtttgccatt gatcgttgat
     3901 gcagctgcgg aagaagattt attcaaatat agcgaaatgg gcgttgactt agtgatttac
     3961 tctggggcaa aagcaatcga aggtccgagt gccggactgg tcataggaaa aaagaagtat
     4021 attcaatgga ttcggctaca gtctaaaggt attggccgag caatgaaaat cggtaaagat
     4081 aatattttag gctttaccca ggcactagaa gattatttaa aaaatgggag cgaatccggt
     4141 gactccatga agcaacgatt agcgcctttt gttaacgcaa tcaatcaact tcctgacctt
     4201 tcagcgaaaa tagttcaaga tggtgccggc agagatattt atcgagcaag tgtaacgatt
     4261 aatggcgata aaagtgcgaa agaagtcatt caggaattaa aagcacgaaa tccagcagtc
     4321 tacacccgag aatatcaagc aaataacgga atcattgagt tcgacattcg ttccgtcagc
     4381 caaaacgaaa tggaaaaaat tataaaaaga ttgagagaaa tcatgtagga ggcttttaaa
     4441 ttgtcagtta tcccaaatta ttataaaaat cgtgtatgtt taaatgtatt agctcattca
     4501 gttgaaaatg caaaagcatg ctatgaagca gcggaagggc atgtcgtact aggtgtgcta
     4561 tcgaaaaatt atgctagcga tgaagcagca atcgaagata tgaaaaggta ccaagacgca
     4621 accaataatg cgttgtcagt tggattaggc gctggagatc caaaccaaag tcagatggtt
     4681 tcgcgtttat ctggggtttt acaaccacaa catgtcaatc aagtatttac tggcgtgggg
     4741 acttctcgtg ctttattggg ccaaaatgat acagtcatca atggattggt ttcaccaaca
     4801 ggtaagatcg gctatgtcaa tattgctaca ggtccgttaa gttcacaagc gccagcagct
     4861 gaagtgccta ttgaaactgc cattcaaatg ctgcaagaca tgggaggtac ttcaatcaag
     4921 tatttcccga tgaaaggctt agcacatagg gaagaattta aagcagttgc ggaagcttgc
     4981 gcaaaatatg aattctattt agagccaaca ggaggaattg atctttcaaa ctttgaagaa
     5041 attctgaaaa ttgcaatcga tgcaggtgta aaaaaagtga ttcctcatgt ttatagttca
     5101 attatagata aagaaacagg agatacgcgt ataggagacg taaagatact attaagcatg
     5161 gtaaaaaaaa ttattcacta actcattaga aaataagaag cttattccag aatgaaatta
     5221 aatggaataa gctttttata attaattacg gaaattgtta atgtagataa taatttagat
     5281 gtacgtaagc agaagtacag aaatgtaaga ttttccaata ttattttatt caacataata
     5341 aatcgtatat caattttttt agcgaaattt ctatttattc caagaatttc agttattacg
     5401 gaatcatttt gtgcttaata aaaagaaacc gtttttatat actataaatg tagaaaggcg
     5461 gtaggaaaat gaaaaaaatt attgcaatca catcctgtcc agttggtatt gcccatacat
     5521 acatggcagc agaaaactta gagaaagcgg ggaaagcagt tggcgccgag gtaaaagtcg
     5581 aaacacatgg ttccatcggt attgaaaatg aattgactgc aagagatatc gaagaagcag
     5641 ctggtgtaat catcgcagca gatactaaaa tagataaatc acgattcggc ggtaaacctt
     5701 tgataaccgt aggtgtgcaa gaaggaatcc accatgcaga tgagttggtg agagatattt
     5761 tagcaggaaa agcgccagtc tataaaagta cggaggcaat tatttcttcc gaaaataaaa
     5821 gtgaatcaga aaatttaggt aaaaaaatct ataagagttt gatgaatggc gtttcgtaca
     5881 tggtaccttt cgtagttaca ggagggttat tgatcgcgat ttctttaaca ttaggaggaa
     5941 cagctacacc tgaagggatc aagataccag aaggaacgat ttgggcaacg atgaatagta
     6001 tcggaagtat cgctatgggg ttgatggttc caattctatc tgcatttatt gcgcaaagta
     6061 ttgcagatcg tccaggactt gttcctggat ttgttggtgg gatgttagcg gcaaacggag
     6121 cattatatgg tagtgatgcg aatgcgggat tcttaggcgg gatcatcact ggatttttag
     6181 caggattcat tgctttagga atcaagaaag tgcgagtacc gaaagcttta caatcgatca
     6241 tgccaattat tgttatccca attttaggat cattggcagt tggttttgtg tttatctatg
     6301 ttattggaga accagtagct cttctattta gttcattgac tcacttttta gcagggatgc
     6361 aaggaggaag tgaaattgtt ctagctatga tcttaggagc gatgattgct tttgatatgg
     6421 gggggccgtt caataaagta gcctttttgt tcggatctgg cttgattgca gaaggcaatt
     6481 attccattat ggggccaatt gctgtagcta tctgtatccc tccattagca ttggggctgg
     6541 catcattgat tttaaaaacc aaatttacga aaacagaacg agaagctggt aaggcgtcct
     6601 tagcaatggg actgtttggg attacagaag gcgctattcc gtttgcttca acagatccac
     6661 tacgggtgat tccaagcatt gtaatcgggg caatgacagg aagtgtgatc gcgatgcttt
     6721 caggagtgac agatcatgta gctcatggcg gtccgatcgt agcggtttta ggagcagtgg
     6781 atcacgtagc catgtttttt gtagcagtga ttgctggtat tgctgtaacc gtgctaatgt
     6841 taagaatcct taaaccgaca ttacaacaaa ctgcggttgc tgcagcaaat ggtgtgacag
     6901 tagcagatct atcagagact gaaacaagtg atgtttatac gaaaactgaa acttttgatt
     6961 ctatcacgga tattatgtca gatgaagcaa tcgttttagg tgaaacagca acaacaaaag
     7021 acggagtaat cgaagattta gtccaacgtt tagaaaaaac agcggcgatc acagataaaa
     7081 ccagatttaa agaagtcatc tatgatagag aacatgaaag tacaacggga atcggtatgg
     7141 ggattgctat tccacatgga aaatcagctt atgttcttaa accaacagtg gcatttgcca
     7201 gaagtcaatc tggggtagat tggcattctt tagatggaaa acctgcacat atgatcttta
     7261 tgattgctgt acctgaaaac agccaaggtg atatgcactt gaaaatactg caacgtctat
     7321 cacgtaaatt gatggatgat gacttccgtc aagcactgat ggaagcgcca gataaaacaa
     7381 cagttcacca attattaagc gaaatgtaac ataagatgaa aaatcgaccg tgatgagaca
     7441 taatagatgt gttatcatga tcgatttttt atttagaagg gagtttctgc acattatgaa
     7501 tcagcgtcag caatctttga tctatcaatt aagtgagaac aataattata taacaacaaa
     7561 aaagctagca gagcatttcc atgtgtcaga aagaacgatt catagcgatt tgaataccat
     7621 tgaaacttgg ttgagagaca aacatctgcc tttaatgttt gaaagaaaaa aaggtacagg
     7681 tatcttgttg aatggtctgc ctcacgaaag ggaacaactg aaacgagcgc ttaatgaaga
     7741 gactcaaaca gaaatggata aaaaacagct tcgtcagtta ttgattttcc atctgctagt
     7801 agcaaaagac ggatttagtc ttgaagaact ttcagaaaaa ctatatgttg gaaaacgtac
     7861 gattcgtaaa gaattgacag agttgcaaag cttttttgag tatcaccatt tacagctagt
     7921 atccaaaaca aaactaggaa cttttcttaa aggcgatgaa caagaaaaaa gacagttgct
     7981 tgttaagaca ttacgaaata tgcagaaaga agatccacaa agtccgtcgt tgaaagaatt
     8041 cttcgctaaa gatacattaa aagttatcca acatacatta caagatgttt tttatgaaaa
     8101 ccatttagaa cagccgaatg gactggctag tgtggagatt cacatctact ttatgctcga
     8161 gcgaatgaaa cagtaccaga aagtgaaatt aagtaaagac gaaaacgaag tcgtggaaca
     8221 cacccaagca caaaagttaa gtagtcaaat attagcaaaa ctagccacca tctatccaat
     8281 tgaattttct cctgatgaaa tcaattatct ggctttgcgt atcgccaatc tttttacaaa
     8341 ttcacaagca gccacacgtt ttcaaaagga atctagcgca cttgctgatc atttgatcga
     8401 acaagtggaa cagtttctag gctattcatt aaaagaagat cagctactaa aacaaaattt
     8461 aaggtctcat ttatcttcaa cttattttcg actccattat ggtttgcaga tttctaatcc
     8521 attgacgaaa aatgtgttta gtacgtacac acaacttttc ttagttttac agttaatttt
     8581 agaagattat tttgaaaagg aaaattttta tgttccacaa gaggagacag cttatttaac
     8641 gatccatttc caagcagcga tcgagcgaca caaacatcag aaaagtagaa gatatcaaac
     8701 ggttttagtt agtgaatatt caaaagctat ggctactttt ctcgaagcac ggctgaacag
     8761 agaacttcca gaattaacga ttatggatct agttgaatat aagccagaat taaagagaca
     8821 agattttccc tctgttgatt ttatcttggc tacgttacca tttaaacata taagcattcc
     8881 agtaatcgag atctcaccaa tgatcacaga aactgatcta gcctatttga caaaatatat
     8941 gctggagcat gtgcctatca agaagaaaaa aacatttgat ctagcaagct ttactcatcc
     9001 atttctgatc tttcctcaac ttgaacggac agacccagcc gatattttaa actttatggg
     9061 aaacgtctta gtggaacatc attatgtgga gccggaattc atcgattctg tcttggaaag
     9121 agacagtcac tccagtacta gagtagcacc atttgtaaca atccctcacg gtaatccgct
     9181 gtatgtgaag cattcgatga tttctattgc tacaatgaaa gaaccagtcc tatggcacgg
     9241 ggaacagata cgtatcgtac tgctattagc aataaagaaa aatgatttga aacatgcaga
     9301 gttcaaaaaa atattttctg ttattcacta tctagaaaag cagccagaac aaatggctca
     9361 gttgatgcaa tccaatcatc cgctagaaat cttaacgttg ttatctagtt atgaataagt
     9421 agcgattaaa aaactgcctt tatatttata ataattctat ctatttattt ggaagtagaa
     9481 aactaaatac aaaaaaagag gatgtggcca aagttgtggc acatcctttt attttgagtt
     9541 tcttataatt ttttatagtt gcttacgatg tcttgactac tggtaaacgg tgttcaagag
     9601 acaatttgtc ggcaataagg cacaattttg taaaatatgg aaagctattt ttttaacatt
     9661 gtttcttagt gcttgaaaaa tcattttgaa tacttcttta tacgatcgat gtacgttgat
     9721 ttaagtctaa aacttttctt ttgattcctt ctctgacttt tctgaattcc gttaatattt
     9781 cttcttcgct cccgacagct tgtgcaggat cttttaggtc ccagtgaagg tgttcgtgat
     9841 tgtttggaac ggcagggcac ttgtctttgg catcaccaca aagtgtaatg atcaagtctg
     9901 cctgagt
//
LOCUS       DACDYJ010000087         9906 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDYJ010000087 DACDYJ010000000
VERSION     DACDYJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.088"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            83..289
                     /locus_tag="IWM51_002399"
     CDS             83..289
                     /locus_tag="IWM51_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9971771.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            289..513
                     /locus_tag="IWM51_002400"
     CDS             289..513
                     /locus_tag="IWM51_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971772.1"
                     /translation="MKTQHKLLNVKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFE
                     DLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            528..965
                     /locus_tag="IWM51_002401"
     CDS             528..965
                     /locus_tag="IWM51_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971773.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEAGVKPSTIKPSEYQALE
                     LTYGKFIETKKAKTVSSEWLDYFENVNKYGACYIMKKEDNENE"
     gene            958..1665
                     /locus_tag="IWM51_002402"
     CDS             958..1665
                     /locus_tag="IWM51_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971774.1"
                     /translation="MSNVVYQLTVEDVSHFLTNKEHLNEVVRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDCKRMPYQRFERKKGQ"
     gene            2046..2225
                     /locus_tag="IWM51_002403"
     CDS             2046..2225
                     /locus_tag="IWM51_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971775.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQELKQKYGKSESRIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            2318..2503
                     /locus_tag="IWM51_002404"
     CDS             2318..2503
                     /locus_tag="IWM51_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, HicA family"
                     /protein_id="HAP9971776.1"
                     /translation="MPLTGKEMLKLLKKNGWVERRQEGSHHHLYKDGVRITVPVHANQ
                     DLGRGLEQKILKDAGLK"
     gene            2537..2968
                     /locus_tag="IWM51_002405"
     CDS             2537..2968
                     /locus_tag="IWM51_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAP9971777.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
     gene            3905..5179
                     /locus_tag="IWM51_002406"
     CDS             3905..5179
                     /locus_tag="IWM51_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9971778.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            5233..6660
                     /locus_tag="IWM51_002407"
     CDS             5233..6660
                     /locus_tag="IWM51_002407"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP9971779.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDHLYMKPVRELEQLRVG
                     SGVETKVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            6673..7545
                     /locus_tag="IWM51_002408"
     CDS             6673..7545
                     /locus_tag="IWM51_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9971780.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNELLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            7640..8635
                     /locus_tag="IWM51_002409"
     CDS             7640..8635
                     /locus_tag="IWM51_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9971781.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKDN"
     gene            complement(9240..9347)
                     /locus_tag="IWM51_002410"
                     /pseudo
     CDS             complement(9240..9347)
                     /locus_tag="IWM51_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            9463..>9906
                     /locus_tag="IWM51_002411"
     CDS             9463..>9906
                     /locus_tag="IWM51_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9971782.1"
                     /translation="MIIKNLGNVKSIDIHVKDLLIFTGKNNTGKTYTSYILYGILSEL
                     FGARFFNYFSHTEIEPHLQNSLNILLVSKDSIRKKFNNDAKKFLEENLKKIAVRNFKV
                     SENNFKNLSFTISDSEISELTGDIFNYEIKRYSISGIVITIQNVGE"
BASE COUNT         3511 a         1418 c         1896 g         3081 t
ORIGIN      
        1 gattcattct ggccaacaaa tcaataaaga aagaactaac gaaaggaagt aaaacctttc
       61 ctaagaggag gcagaatcaa tcatgcgaaa gaaatttgat tattggggtg ttcccttcag
      121 cgaattattc ccaaactatc atgcaccaca tactgtcgaa tgcgattgtg gagaaagagc
      181 gaaatgtatc aaaagctatc gtttgtatca atgtccaaca tgcgggaaaa aatatacctt
      241 gtcttatggc gattacattt tgattgatga gaaaggaaag aaacgctgat gaaaacacaa
      301 cacaaattat tgaatgtaaa agaagtcatt tctgaaaaag tagcagaagc tttacttatt
      361 ccgcggtact ggacgataga aaccccttta gtagaggacg caccgactat gctatttgaa
      421 gacctagtga agattgcagg atatgaactg cattatcaag aaaacttgga aacttatgaa
      481 aacgaatatt ggttaaccaa attagagaac taaaaaggag aaaaaatatg gctcagaatt
      541 actatgacga atttgtcaaa ttacctttgg ataaaatggc acaaaaaatg gaggatatga
      601 cgttcctata ccatgaaaca cgtgtaccca aaaaacatta caaggaaaaa ttgtcagtcg
      661 cagtggaaga aatgattgaa tcaggtgtcg aaatgaatct cattgcgacg tactatcgaa
      721 cattggaaga actgaaaaaa caaaatggaa aatggttttt ccaagcctta ctatgcttgg
      781 aagcaggcgt caaaccaagc acaatcaaac cttctgaata ccaagcttta gaactaacgt
      841 atggaaaatt tatcgaaacg aaaaaagcca agactgtttc aagtgaatgg ttggactact
      901 ttgaaaatgt caacaagtat ggcgcatgtt acatcatgaa aaaggaggac aacgaaaatg
      961 agtaatgtgg tttatcaact aacagtggaa gatgtttctc actttttaac aaacaaagaa
     1021 catttgaatg aagtagtcag acggattcaa aaagacttta gttgtatgga agaaatgaag
     1081 tactttattc aaagagtgga agaacagttg tatatggaaa gcgaaacgag tcaaacgaag
     1141 gtcacagtca gccataatca tttagacgtg tataaacgtc gtcctgaaac ggtaaaaaaa
     1201 ggaacagagc ttctacgact agaaacagaa aaatacatca tgaatgtgta cctagaaagg
     1261 agtgagctgg tcgaaaactt tgtcagctac acgatttgta ggaaacacta ttactcagaa
     1321 tcagaatatt attcaatttt tgagagcatg gaattaatgg aagaagaaat ggaagagcta
     1381 aacagcaaaa atgcgacggt agacgattat taccttgtat ttactagatt tctgacgtgt
     1441 gggacggaat ctatcgtgtt aatgggaatg gattatgaac tcgctgtacc cgaagcattt
     1501 ttgagggaat atgtagaaga agtagataac ccaggcgacg actggtacac gactttaact
     1561 gattttttag atacctacac ttacgacgat acagaagaaa tccaaaatga gcttgactgt
     1621 aaacgaatgc catatcaacg atttgaaagg aaaaaaggtc aataaccatt tataccgaaa
     1681 agtgagaatg tgtgaaataa gaacaaaaaa ccttgaaatt aagggaatgg ttcttatttc
     1741 gcaccaatcg ctccaaccgc ccccgccgct caggtggtgc gaaataagcg ggcaacgacc
     1801 gataacctaa gagaaagaag gaaatccata tgaataaaat cattcctctg ttgttcgtac
     1861 tgtttgttgg cgagatactt atgcgtcatg ctgcagctgt acatttaaac ttcgtagtat
     1921 tcttaggaat actggtattt tgtttgatct tttggcgatt tgtattagcc cttgtgagtc
     1981 tactattcaa gatgattgca gccatatttt tcatagggtt atttcgatga aaggagagag
     2041 tactaatgcc aattgcaata ggaaataaac gtttacccgt gacgttggat gaaaagagac
     2101 aaaaagaatt gcaggaactc aaacagaagt acggcaaaag tgaatccagg attatgtgta
     2161 ttgcgttaga tttattgatt gcacaagaaa aagcagggtt tgatgtacca gcactcaaaa
     2221 agtgacgtca cttttcattt gaagaaacaa aagtgatagc actttacgca cgtgtcgata
     2281 cgtgttatga ttattttgta aggaggtata gtgcacaatg ccacttacgg gaaaagaaat
     2341 gcttaagtta ttgaaaaaaa atggctgggt tgagcgaagg caagagggat ctcatcatca
     2401 tctttataag gatggtgtta gaatcactgt tcctgttcat gccaatcaag atcttggccg
     2461 aggacttgag caaaaaattt taaaggatgc gggattgaaa taatctcgca ttcgattaaa
     2521 gaaaggattg actgaaatgt taagtgagcc tttagcaaaa acctatcctg ctatttttag
     2581 cccagaagaa ggtggtgggt actttattga atttcctgat gttcaaggtg cttacacagg
     2641 aattaacgaa gacgatatat cttatggaat tgctatggca caagaggtgt taggtatggt
     2701 tttagcagat tatattgagc atgaagatct acttccagaa ccaacaccaa tcaataagat
     2761 ttctgttgaa gatgattctt ttactacttt aattcgtgta gatgttgcca aatatttgaa
     2821 agacacagaa cttgtcaaaa aaaccttaac aattcctcaa tgggcggata agctaggcaa
     2881 aagggctggc ataaattttt cagtccttct aacagaatca attgcagata aagcagataa
     2941 cattcttcgt tctggaagaa ataattagaa ctcaaaagag tcagaaactt aaaaatcttt
     3001 ggcttctttt tttactacaa agatggcttc tttaaattat ttgcctgatt gatttaaaaa
     3061 aattgcgcaa agactacgag ctaattaagt tgctcgtggt ctttctttat gaaaactttt
     3121 tagatctaga taaaaacatt gagacacttt gaaaaatttt tttaatagta atgggcgctt
     3181 ctatgtcaag ctgattcttc tttcggtaaa ttaattttac ttagaatatc actatcttga
     3241 taaaaaatgg tgtattcaca aattctggca taaaaaatac ctactttcta gttaaggttc
     3301 ctgaataagt cgcaatccaa ttatttccag ctgatttata actttttaaa tataattttc
     3361 cataatacca ataaccatta cttttttctg tccaatttcg ttattgagaa gatctagaag
     3421 gccagccaac tttatcaaaa atcatcattc ctgtcactcg tatcgtttta gaagctgctc
     3481 gagtaagtaa tggagaaact gttaaataat tttaagtaac agttaagtcg ttagttgggt
     3541 tatcaatggc tattccgtcc ttatctgaac ctcgaaaccc aatcatagaa aaagtttttt
     3601 gaacaagttt taacagataa atcaagttat atactagaaa attcgtaaaa tatattttta
     3661 atgtcataat cgtttgattt ttctggattg acatttattt ataaaaaata gtgccattaa
     3721 aaaagaaaag tgacacacca ataaaagtca tatcctgtga cattatatgg cattatccaa
     3781 atagaataac cggttgacat gtaagcggat tccttttata attttaggag tataagtcct
     3841 tttctaaaaa tattaaccgg ttatcatgaa aggggataca aatatatcgt tggaggataa
     3901 aaatatgtca aaggttaacg tatttaaaaa tcaatcctat ttacaaagtt cagctacatt
     3961 attactattt tttgcttctt ggggtgtttg gtggtcattt tttcaacttt ggctaacatc
     4021 tgaatcaaat ggtttagggt tatctggcag tgctgtagga acagtattct cggcaaattc
     4081 gttagttacc ttaattttga tgtttattta tggaacatta caagataaat tgtatattaa
     4141 acgaaattta ttaatttttg cttctgtatt agcgacactt gttggaccat tttttatatg
     4201 gatatacggg ccattgctag ataacaattt taatttaggc attattatgg gagcactatt
     4261 tttgtcagct ggatatttag cttctgtagg agtttttgaa gctgtgtcag aaaggtttag
     4321 tcgtttattt ggctttgaat atggacaagc aagggcgtgg ggatcatttg gttatgcctt
     4381 ggtagcgctt ttggcaggat ttttatttgt aaaaaatcct catttaaact tttgggcggg
     4441 atctttcttt ggttctttac tattgttaaa tttattattt tggaacccta aagttgaacg
     4501 ggaagcaaat caaaatttta atcaagaaca agctgaatca aatagtattc cttctttaaa
     4561 agaaatgttt gatctaatga aactgcctca attatggacg ataatcatct ttattgtttt
     4621 tacatggaca ttttatacgg tattcgatca acaaatgttt ccgggatttt atactggttt
     4681 gttttcaaca tcagctaatg gtgaaaaaat atatgggaca ttgaatgcta ttcaagtatt
     4741 ttgtgaagcg ttaatgatgg gaattgttcc aatcattatg agaaaattag gggttcgaaa
     4801 tactttgtta ttaggtgtaa ccattatgtg tgtacgaatt ggattgtgcg ggtttgcctc
     4861 gacaccatta tctgtttcat gcataaaaat gttgcatgct ttagaagtac cattatttac
     4921 attaccaatg tttcgctatt ttacacttca ttttgataca aagctatcag caaccctcta
     4981 tatgatagga tttcagatag ctgctcaaat tgggcaagtg attttatcaa caccattggg
     5041 aatattaaga gacaacgttg gctatcaacc aacatttaaa attatttctc ttattgtatt
     5101 actagcaggc atatatgcat tctttattct taaacaagat gatagagatg ttcaagggga
     5161 tccatttatt cgaggataat ggaaaccaac agaataaaaa acaattttat ctagaaagtg
     5221 agcgaatata ttatgacaaa aagagctata gaattaacaa atacacgtta tcgtttggga
     5281 tatcatgtgt ctgcaccatc aggatggatt aatgatccaa atggattttg ctattttgat
     5341 gggtattatc atgtgtttta tcaacatcat ccatatagtg ctgaatgggg accaatgcac
     5401 tgggcacatg ctagaagtaa ggatttagtt cattgggaat ctttaccact tgctttgact
     5461 cctggagatc aagaagatga aggtggttgt ttttcaggaa gcgctattga aaaaaatggt
     5521 gtgttgtatc tgttctatac aggacatcac tattatggag acaatgatcc agatcatttt
     5581 tggcaaaatc aaaacatggc gtatagtacg gatggcattc actttactaa atatgaaaaa
     5641 aatcctgtaa tagcaaaagc accagaagat aacacacatc atttcagaga tccaaaagta
     5701 tggaaacacg atgaaaaata ttacatggtt ttgggtagtc aagaaaaaga tggattaggt
     5761 cgtgctattg tttatcagtc gttagattta ttaaattggg attacattgg accaatttct
     5821 aaagcgaagg aagtacttac agaaggattt atgtgggaat gcccagactt ttttgaatta
     5881 aatggaaaat ctattctttt attatcccct cagggaattg aagcaaatgg aaaagattat
     5941 ttaaatctat atgaaacagg ctactttatc ggtgattatg attatgagac agcaaaattc
     6001 attagaggaa atttttatga attagataag ggacacgatt tctatgcaac acaaacaact
     6061 ttatctccag atggtagacg tattgttatg gcttggatgg atatgtggga aagccctatg
     6121 cctgaaaaag tagacggatg ggcaggagca ttaacgcttc ctcgagaatt agagttaatt
     6181 ggagatcatt tgtatatgaa acctgtgaga gaattagaac aacttcgagt tggcagcggc
     6241 gttgaaacaa aagttaattt ggcaggagaa ataataatcg ctcaagatgc ttcattcaaa
     6301 gaagctctaa ttgatgtact attgactaaa tcaaaaaatc aagaaatcaa attttcatta
     6361 aaaacaaaag atgaagagtt aattaatatt ttttattcta aggataaaaa tgaatgtacc
     6421 ttaactcgta acggcaaaaa tgatccaagg tatggaacca ttcagatgtg tgatagatta
     6481 tcacttcgag tgtttatcga taaaagttcg gcagagattt ttattaatga tggcgagctt
     6541 gtttttacag agcgttatta tacagaaaat caaccaagta tgcatttgag tgttagtgaa
     6601 gaagcttttg tctcgagtac aatttatcat ctaaaaaaag atgcaatttc ttacgagtaa
     6661 ggaggtttta tgatgttaat tggtagcata gaagcaggtg ggactaaatt tgtatgtgcc
     6721 gtgggagacg aggattaccg agtacaagac caaataattt gtccaacgac cacccccagg
     6781 gaaacattga aaaaaactgt tgactatttt aaacagtttg atataaatgc attaggtatt
     6841 gcatcatttg gtccaataga aattcgaaaa aattcaccaa aatcaggata tattacgtct
     6901 acaccaaagg tgggatggcg aaatgttaat gtgttaggtt atttacaaga tgagctgaat
     6961 gttccaatgt cttggacaac ggatgtgaac ggatcagctt atggagagta catcatgtcc
     7021 actttatcga atgaaaaaat aaattcatta gtttattaca ccgttggtac tggcgttggt
     7081 ggtggagctg ttgttgacgg aaaactatta ggatcgtttg gtcattcaga aatgggccat
     7141 acgtttgtaa aacgccatcc tgatgatatt gattttaaag gaatttgtcc ttatcatggt
     7201 gattgtttag aaggcttagt tgcgggacca acgtttgaag cacgttttgg aaaaaagggt
     7261 gaagacgttt ctttaacgga tcctatttgg gatgtaatgg cctattatct agcacaagct
     7321 gtgattcaaa caacattgat tatacgaccg gataaaatag tatttggtgg aagcgtagta
     7381 aatgaaaaat tgttagaaaa aattcgtgtt aatgttaatg aattattgga tgattacgtg
     7441 gaaataccac cgttaagtaa atatattacg cgtcctttaa ttaaaaataa tggatcggca
     7501 acactaggaa actttgcttt gggcttgaat caattacaag aataaaacat agaaaaaata
     7561 tcgattatca ggtatagtag ttctatagag ttcttttttt cgatagttaa ttattgcatt
     7621 atttaggaga gacaagagaa tgcgcccaaa attagaagat gttgccaaaa gagcaaatgt
     7681 atcaaaaaca actgtttcac gtgtattaaa caatcgtggc tatttaagtc aaaaaacgat
     7741 tgataatgtt tataaggcta tagaagaact caattatcaa ccgaacgttg tagcgcgtca
     7801 attatttcaa aagaaaacta acattgtcgg gttactattt cctacagtag ccaatccttt
     7861 ttttagtgaa ctcgttgagg cattggaaaa aaagttatat gaaattggtt ataaggtatt
     7921 aataggcaat tcgatgaata ataaagaaaa ggaaacaaat tatttaaatc agttattgtc
     7981 tgatcaagta gatggtttaa ttgttggtac acataaccaa gggattcagg aatataaata
     8041 tcagaattta ccgattgttg caattgatcg agtgatgaat gaagatattc cagttgttga
     8101 atccgataac tacaatggtg gaaaacttgc aaccaagtta ttaattgcac aaggtgcaaa
     8161 aaatattatt catacaaacg gtccaattga cttacagact ccggctaatc gtcggagact
     8221 agcctatgaa gatactatga aagcatatca gttaattcca cgaactgtga cattagattt
     8281 caatatttct tatgtaaaga aaaaacagat ttttttccaa atgtttgagg attatcctaa
     8341 aatagatggt atttttgctt caaatgacat cgatgcggcc ctaatattac aagtagcaaa
     8401 agaaaagggg ttaaatgtac cagctgattt attagtagtc ggatatgatg gtactttaat
     8461 gactcggagt atattaccag atctgacaac agtaatacaa ccaattaatg atatagcaga
     8521 cacagctgtt gctattttga tgaaacgaat aaataaagaa gaaacaaaaa aggaatatat
     8581 tttacctgtc acattatgga tggggaagac atcagtaaag aaaaaggaca attaaattta
     8641 taaagactat caatgataat ttcattatta attcgtgata taaaaagttg ttaaatctct
     8701 tataatagac tatgatacaa aatcatgctt ttaatctgtg tagatgttgc caaatatttg
     8761 aaagacatag aacttgtcag aaaaacctta acaattcctc aatgggcgga taagctaggc
     8821 aaaagggctg gcataaactt ttcagtcctt ctaacagaat caattgcaga taaagcagat
     8881 aacattcttc tttctggaag aaataattag aactcaaaag ggtcagaaac ttaaaagttt
     8941 ttgatccttt attttcaagt ctgatttgac agattttcgg cagctcattt atttatggtc
     9001 agaaatagtt gctgaaattt taatctatta gcaaatagtc cagaacgtat gaagaggatt
     9061 cggatatcat ttgtcattaa caccataaat atttataaaa ttcgtgttca cctacaatta
     9121 aatttaatat ataatattcg ttgtaagggc ttataatttt ttgttaatga acaagatatg
     9181 taattgatag tgtcaatagc tattcaaaaa tgtaggcttt tggcccataa ataacaaggt
     9241 tacttatatg ataacttttt ttctttataa tctttaatcc gatagagctt tccagtagtt
     9301 tgaataatct tagcgtggtg cactaatcga ttgattagtg catttgtgtt cagtccattg
     9361 agacataata gtggatagtt ttactgagaa aattgataaa aaatagagat ataattcaaa
     9421 tacgtatata atgaaactaa agataaatac ggaggaaaat atatgattat aaaaaactta
     9481 ggaaatgtta aatcaataga tatacacgtt aaagatttgc tgatttttac tgggaaaaat
     9541 aatacgggaa aaacatatac cagctatatt ttatatggaa ttttatcaga actctttgga
     9601 gcaagatttt tcaattattt ttctcataca gaaattgaac cacatttaca aaatagttta
     9661 aatattttat tagttagcaa agactctata aggaaaaaat ttaataatga tgcaaaaaaa
     9721 tttttagaag aaaatttaaa aaaaattgca gttcgtaact ttaaagtaag tgaaaataac
     9781 ttcaaaaatc tttcttttac tatttcagat tcagaaatta gtgagctcac tggtgatatc
     9841 tttaattatg agatcaaaag atacagtata agtggcattg ttataacaat tcaaaatgta
     9901 ggagag
//
LOCUS       DACDYJ010000088         9736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDYJ010000088 DACDYJ010000000
VERSION     DACDYJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.089"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1478..2632
                     /locus_tag="IWM51_002412"
     CDS             1478..2632
                     /locus_tag="IWM51_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP9971783.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            2626..3048
                     /locus_tag="IWM51_002413"
     CDS             2626..3048
                     /locus_tag="IWM51_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971784.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3350..3730
                     /locus_tag="IWM51_002414"
     CDS             3350..3730
                     /locus_tag="IWM51_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9971785.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            3712..4626
                     /locus_tag="IWM51_002415"
     CDS             3712..4626
                     /locus_tag="IWM51_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9971786.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            4866..5555
                     /locus_tag="IWM51_002416"
     CDS             4866..5555
                     /locus_tag="IWM51_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971787.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQVLAQKIEKFTA
                     CYEDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKV
                     EEACSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(5675..5761)
                     /locus_tag="IWM51_002417"
     CDS             complement(5675..5761)
                     /locus_tag="IWM51_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9971788.1"
                     /translation="MFELFICPLLVGVCISVFSYWLNNRNKK"
     gene            6407..7339
                     /locus_tag="IWM51_002418"
     CDS             6407..7339
                     /locus_tag="IWM51_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9971789.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIH
                     GEVDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEAD
                     FKKQQQTEKEEAEKQSISNELKQDLLENLQNLFD"
     gene            7356..7826
                     /locus_tag="IWM51_002419"
     CDS             7356..7826
                     /locus_tag="IWM51_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9971790.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTL
                     VVSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLD
                     QQQQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
     gene            7910..8214
                     /locus_tag="IWM51_002420"
                     /pseudo
     CDS             7910..8214
                     /locus_tag="IWM51_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            8489..9670
                     /locus_tag="IWM51_002421"
     CDS             8489..9670
                     /locus_tag="IWM51_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9971791.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKI
                     TKELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVL
                     TGAKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIAT
                     LPIGILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISD
                     TGRDFLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNI
                     HVPVRESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVA
                     MIFFHVFENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQ
                     K"
BASE COUNT         3617 a         1549 c         1601 g         2969 t
ORIGIN      
        1 gttagtaaat attagaggta aacaaactat gcgtacttgt taccttatga gttggaatgc
       61 tagtagtaga acagccaaat attattacta catttagtaa aaacaaaaca ttctttctat
      121 acaataaaat agcgtataga gaggatgttt ttaatttaaa ggagtggtta tctttatgaa
      181 aaatagatca tttgctatat tagtattatc cgtaatttta ttagatatat tgttaataac
      241 attagcttta aaaactttaa atttagtaac aagttttgtc attataccag taattatagg
      301 aatgtctatt tatttcatca aaaaaaataa acaatttttt tgaatgaaat tacgggcgaa
      361 aataatcact caaaattatt aataaaaacg aatcaactac tttttctgaa gaaacataca
      421 accgtaaaca acaattgttg tttacggttg taaattatca aactatatgg tgacttgttg
      481 cttaaaatag gttgaaattt cgtttatttt ttttgagaaa ttttacccac gtactaccca
      541 tgtacctatc cacgtattac ccatgtaccc ccccatgaca aatgggcgga ggggcttgcg
      601 gcgcttggta ttaaaggcat tatccgcctc ccaggtttct gaccattttc tctcataagt
      661 ggggttttta ccgcgttgag agaaaatggt cagaaaccta aacgatcggc aaagaaaaca
      721 taccaagcgc cacaagcaaa aaaagttaga gtaacttcca gaaaagaaaa atttttagaa
      781 caacttttga aaatttgatt ttgatttata tccgttcaat ttaaacattt gataaactga
      841 aggctagtat ttaaaatgtg tatacaagtg gtagcgttaa aaaaagtgga ggtataacct
      901 atgttaaaaa aaactgtgtt ggtactgggt tctatttctc taggaatatt aggttttagc
      961 cccatgctat tatccgtaat tggaaaaaac aattccaaca gagaacttaa attaaattca
     1021 aattattaca acagtcctaa cgaagaattg tttgtaaaac aaacggataa ataataaaaa
     1081 agactgctct ttcgagcagc cagttaaaaa ataatgagtg tttattcttg aaaaagcacg
     1141 cgcttatgaa gcgctaccaa aaatttacca tattttacaa aaaataacaa gtcttaaaaa
     1201 aatgacagat aaaaatgcac aaagaattag atatagataa ccgcgaaaaa tcaagcttta
     1261 gaaagttgct tttagctaga ccaattaaaa atcacaaagt gagcgtctct atccattggt
     1321 agaggggcta cttggcatag tcgacattat gttaactaac gctataaata acgattatgt
     1381 tcccaaaaca atattttatg aactataaca aaaatttgtt atcgcatatt gtatatttta
     1441 attataggtg tataccactt aaatttaaca caaaggcgtg attattttga aacccaaaaa
     1501 gaaaatttat ctaggtctcc tcttttcctt gatgattgga atttttagta taggaattac
     1561 aaatacctat gcttcagaaa attctaattt caatatcaac tcccaaccaa ttgaagaaca
     1621 agcaattaat tttgcgaaaa ctgattttga acactccata acttcaattc ttttaatgga
     1681 aaataacacc gaaactgata ttaactatac tcttggttcg ccatttaaaa tgctcgatgg
     1741 agaaaatacg gcaaatagtt ctattttctt tccagtattt tcagacgctg ataatacgat
     1801 taaatatatt tataccgtca aaaaaaatac aaatggagaa tattcaggaa gtatttcaca
     1861 atttttagca aaagaacttc aaaatctagt agatagtaat ataacagata caaccttatt
     1921 ttttcaagat aatagcattt attattccga taatggacaa attaaactac tttggaaatc
     1981 acctttaagt acagaaaaaa atggtgtttc ttcctttaca gaaaaaaatg atcttatatc
     2041 cgtaaatata aaaaataaaa ttaaaaattt ctctaaaaaa caaaattttc ctgcgattaa
     2101 acgtgtaaaa agtgtctcct ccggagataa ctttattatg attaattggc gtatagcaga
     2161 aacccaaaca agttttccgt ggtgtgctgc ttacgtagaa gctgctatct tatgtaacaa
     2221 aacagatgta catgctactt cagctagagc cattatgcat tatacctatc ctaatttaaa
     2281 caacagtcaa attgaatcaa aaagtcttaa tcaagatcaa ataataaaat atgcaaatta
     2341 ctgtggtgtt tatcctaaaa aagtagcacg aatactaaac ctttcagagg tccaaacaca
     2401 actaagaaaa ggaaatgcca tttatatggg aactgctggt tatggaaatt atagtaacag
     2461 ccgtcatgca cttgcattat ttgggtgggt caatcaaggt ggttatcaaa cttactatgt
     2521 gtggaatcct tggtataact acccaactgt aataagtgca aatgcaggac cagttacaat
     2581 tcctgtacct ggaggtggtt ttacttggga caataccatt acaaattggt aaataaaccc
     2641 attgctttag ttatgggtct agtgttagtt cttttcttaa ctagttgttc aaaaaaacaa
     2701 gaaatacaaa agactgatct gattcctttt ctaggaactt ggcaaactaa agatcattca
     2761 gaacgtctag taatcactaa tttaaatact tcaaaaaata tagaacaata taccatctta
     2821 gtaaaagaga acaatcataa aattcaaatc attgataaaa atttaaatat aaatgaacaa
     2881 gttttcattt acaaagcaac aaataattta ctaacactgc gctctaataa aaaacaatta
     2941 gattacactt ttagtttgga ttctccaaaa aagctcgtat ttattttgac aacgaatgat
     3001 gggacagaag ggacagctaa accaattgaa ttttttaaag ctgattgatg aatagttgtt
     3061 tgatattaca aaacaataat ttcaccgaag gtgaaacaag cgaaacttta caaaaagtgg
     3121 ctagcatttg ctagcctttt ttgtattgtg gagcgtgggt tttgaggggg attcaagggg
     3181 gagcttgcgc ccctttgaaa agcggttatt tgcaccaaat acgtaggtat ttggctagca
     3241 aatatagagc ttgccaaacc cttttagtga gctttactca ttgtaccttt ttaggtacaa
     3301 tggtattgcg aacgatacgt ccgtaggacg tactgaaagg gtgggactta tgagcgaaca
     3361 aaatcaaaat ttggctagcg atcattctaa aaaatatacc tatcgatccg agccaaaaca
     3421 aatcagtttt cgagtgagcg aatctgaatt tgcaaagctg aagcagtcag ctgaagcttt
     3481 gcaaatgagt gtgccggctt tcgtaaaagc caaggcacaa aacgcgcgtc tggtgacacc
     3541 gaaagtggcg ccagacattg cccaggcaat cgcgcgcgat ttagccaaag ctggagggaa
     3601 tatcaatcaa attgctaagt ggtgtaatac acaccaacac gacgttgctc ctggagacgc
     3661 ccaacgctta tccgaaaacc taaaaatcat gcaaaaggag ctacaaaaga tatggcaaca
     3721 gttaaagtaa gccgaaccac ttcttgtagt cgtgcaatta attacgctga accacgagca
     3781 accattaaaa caggaataaa ctgtgacgtc gattatgcca aaagtgaaat gaaacaaatt
     3841 cgcatgctgt atggaaaaga cgaccgtgta caggcacact tattgattca atcttttcga
     3901 ccaggagaaa ttaccgctga aaaagctaat caactagggg cagagtatgc aaaaaaaata
     3961 gcccccgatc accaaatagc tatttacaca catacagaca aagaccatat tcataatcac
     4021 attgtcatta actccgttaa tctggagact ggaaaaaaat ttcaagctca tggtcaagca
     4081 tttctaaaca aatgttatga cctaaatgac gagatttgtc ttgcacataa cttaccaatc
     4141 actgagcgag gaaagaaacc agaaaaacga accatgtctg aaataaaatt aaaagaaaaa
     4201 aatgaacctg tttggaaaga cgaaattcgg attgcgatcg atcaaacaat gaaaaacaaa
     4261 aaaaccagaa cctatgacca attttgtaat tccctcaaaa cattggggat tcattgcttc
     4321 aatcgcggga aaaatttcac ttacgaattg atccacaaaa agaaaaaagt acgatccaac
     4381 agactaggaa aggactacga aaaggagacg attttacatg agctggacag acgagaacga
     4441 acctataacc aacagcgaac caacgagcta cgaagagctc gagctagcat tcgcgaaact
     4501 gcagaagaag tacgaagaga acaacgaact cttacgcctg aacaaccgga aattagaccg
     4561 gctcgaagaa accatgaaca acacgttgaa agaggaacag accaccaaca agaattatcg
     4621 ctctaaaacg ttaaaagaca tagaaaacga aataaagaaa caacgtgctt ttctcgttcc
     4681 ttatacgcaa gaaaggatac aaactacgat tgatttacaa gttaaaaaac tccatcaaga
     4741 gctattagac cagcgaaata aggaacagaa aaaggtgctt attcaacgat cattggattt
     4801 tcttgccccc ttatgttgct tagtcctgac actgttcgca ttttggttag tatggggagg
     4861 ctaaaatggc attttattta ccctatctac tcatttttgt tagtatttca ggaagtattt
     4921 ggctcatcta taaaattttt caaacaagat attcattaaa aggctctaaa attcgattta
     4981 agcgattttt ctttcttggc tgtatatttt ccttgataat cgtttcaagc ggcttattcg
     5041 gcgttttaga gggaaataag cgcgtttcta gaagtatttt attaggaaat gtcactcaaa
     5101 agtatgaatc agcgcgtgat aaaaagaaaa aagagcaagt attagcacaa aaaatagaaa
     5161 aatttaccgc ttgttatgag gacatgaatg atatttttgt gaagcaagaa aagcgtttga
     5221 cggataaaaa tatggaaaca ctcactcgac tttatcggaa tttgccagaa gaacagcaaa
     5281 aagagtacca agaaaaatat gaacaagtaa aaaaagacgt gcaatatgtg aaagatacga
     5341 aagtggaaga agcttgtagt gatttatttg gagatacaat tcctttttca actagcgaac
     5401 aagaacgaaa agaacgacaa caatcagtca catatgaacg ctatgaaatc ttgcttcaac
     5461 aagcaattaa catacaaaat ctgactaaaa aagaaacagc tttaaattat ttaaaaagcg
     5521 tcaaggaatg gttagatcag caacagcaaa attgaatttg attgaaattc atgttaaact
     5581 aaacatgcaa atgttcaaac attagataca cgaaagcccc agagaggtcg gattctctgg
     5641 ggcttttttt aggttgatgt cgcctttact gattttactt cttgtttcgg ttattcaacc
     5701 aataactgaa tacagagata caaactccta caagaagggg gcaaataaac agttcaaaca
     5761 tcagatacac ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacccta
     5821 caaaattaaa tctgtgtggc aattttaagt atctcttatt tccgggttgt tagggcttgc
     5881 cctgaccgcc tgtagggcgc tttaaatgcc agataaaggt ttttatacta agactgtctt
     5941 aaaacggctt agacaagctc ttagagcctc aaccctcctt tttgaacaat ggttttagca
     6001 aaggagctgg aggcgactgc gcaggtataa tgtgagagcg tagcgatcac acctttttgg
     6061 cgaccgtagg gagcgaggtt ttgaattagc agtttagaaa agttcgcgaa cctgtttaaa
     6121 gaaaaaataa ttgttttttt aggtgggaaa ttttgtctcc ctcttttata atcttttttt
     6181 aaacctcttt ttaaacctct ttaccgtcgg ctgtgtcctt actcccccaa gggatacaaa
     6241 attttatagg catgaaatta tctgtttata ggcatgaaat tatctgttta taggcatgaa
     6301 attatctgtt tataggcatg aaattatctg ttttaggtat tgtttttaga cataatatgt
     6361 cctaaaatga tgtcaataaa atcatttaga ctggagtaac aactaaatgg caaatgaaat
     6421 cgtaaagtat gaaccagaat taaataccat tcctttaaga aaatttaccc caatagaaat
     6481 gaacttgttt ttttctattg tctctagaat gcgcgataaa ggtaatcaaa aaatacgctt
     6541 ttatttcagc caacttaaag atttaagtgc gtataagcag actgcaggag aagtctttat
     6601 aaaagatttg gcaagaacat acgatcattt gatggattta cgtttcggaa gaaaaagcaa
     6661 tggtggatta acaatagaac gatttacaat gtttactgat tttgaaattc atggagaagt
     6721 cgataaccct tatgtagata taaaagtcca cgaaaaagct ttgcctttac ttaataatct
     6781 agaaagctgg gtacgtttct ctttaacaga atttcgagat ttaaatagca gttatgctaa
     6841 aacaatgttt agattgctaa aacaataccg aactcaagga tgggctgaat tttctaagga
     6901 agatttttta gagctattag atattcctaa aagctataga caaacaaata taaatcagtt
     6961 tgtactcaaa ccaataaaag aagaactaac tccactattt aaaggattaa ctattcgtaa
     7021 aaaatatggt aaaggtcgag gtaaacctgt tattggttat cgttttactt ggaaagctga
     7081 aatcaaccat gctgacgatt tttcaaaagg gaagcaagaa gatttacgta taaaattatt
     7141 taatattgaa cacaatggag aattaacaca aaaagaaaaa tggcgtgcta aagatcgaat
     7201 actaaatctt ccgttaggta ctcatgaagc tgattttaaa aaacaacaac aaaccgaaaa
     7261 agaagaagcg gaaaaacaat caatctcaaa tgagcttaaa caagatttat tagaaaactt
     7321 acaaaactta ttcgattgaa gcataaggag tatttatgat agataaaacg atcaaagaac
     7381 tcgcggaaga atttcatgtt tcaaaacaag caattaggaa aaggctatca gctgatttta
     7441 gagaaaacta tgtgcaaact gttactagga acggagtgaa aaccttagtt gtcagctaca
     7501 aaggatattt gcttttaaaa caacattttt taggtagtaa caccactggt aaccaagtag
     7561 aaaccgttgc tagtaacgat gaaaactcga ttattgtttt tttacatgaa cagctccaag
     7621 aaaaagatac tcaaataaaa caaatgcaaa agttactcga ccagcaacaa caactcacct
     7681 tgcaggcaaa tcaacagatc gaaaaactgc aagaacagtt acaattagct tatacggaag
     7741 aacctgaaga agaggaatta acttctgtca ataaagaaaa caacaatgca ataacaacag
     7801 aaaaaaagtg gtggaaattt tggtaacaat taccaacttt ccataaagaa gaaatcaatt
     7861 tctatagact ctatctttct aagaataaaa aatatgttta ggtgatcaaa tgactataag
     7921 taaagtaatc aaacaaaaaa gaatagaaaa acaactaact caagaagatt tagctgaaat
     7981 gcttttagta tctaaaaaaa ccatttctaa ttgggaaaat ggtcgaacaa taccagatac
     8041 agaaaattta attcagtttg ctaagttatt tgatttatca ctagacaata ttttgttagg
     8101 agaagaaaaa attatggtaa aaaatcatac aaaaacacta atactattat ttcattttgt
     8161 aattttttta agtttagttt tcaccttaat tcagtccgga ttcttttatt ttagccattc
     8221 aattgccact tggttattaa tattttttct aagtttacta ttaataatta atactttaat
     8281 tattatcttt aataaacata gaaacttatt tcttcacagc atatttccaa ttgttttatt
     8341 aatttttcta tactttatat aaaaagatag aactatttag taagataaaa aagtagctaa
     8401 ttgtaatttt cgtctaaaac tcaaaactag taaaaataac tttattgtgt tatttttatt
     8461 tataaattat gaaaaaggac ctttcgaaat gaaaaataat agaatctaca aaaatcagtt
     8521 gaattatggg cttttattgc cagtttttct gctatgtatc attggattac tttctttata
     8581 tgttgcttta tatcacgact cttacactaa ttcaaaaatc acaaaagaat taattaaaca
     8641 agtattgtgg tatagtattg ggttcacttc agcaataact atttattttt ttaatcctaa
     8701 aattttgtgg aaattaactc ctatatttta cggattatgc atcataatgt tattacttct
     8761 cttgaaattc tataatccac aattagcagt cttaactgga gcaaaaaatt ggttttatat
     8821 tgctggtttt tcgtttcaac ctactgagtt agtcaaaata ggatacaccc ttatgctagc
     8881 taaaataacc gtcaattatc agcaaaaatc tattactagt aatacgaaag atgattggat
     8941 ttatttactt aaaactttta ttgctactct tccaatagga atattattat tttttcagaa
     9001 cgatctagga acaatgttag tcttcattgt tatttatatt tttattttgt ttacgtctaa
     9061 cataaatata aaaataattg ccccaataat cgccattggt gttgttttat ccttaatatt
     9121 tttatatttg gttatatcag atacaggaag agatttctta ttaaaaatag gatttcacga
     9181 ctatcagttt aaaagaatag actcatggtt gaatccattt ttagatccaa atggatctag
     9241 ctatcagtta gcaaatagtt taattgctat tggctctggc ggacttttag gtactggttt
     9301 taatgtatca aacattcacg ttcctgttag agaatccgat atgatatttt ctgtaattgg
     9361 agaaaatttt ggctttatag gaagttgtct tgtaatattc ctatactttt atttaattta
     9421 tcaaatgtat aaaacatgtt ttaaaagcaa taacttattt ttcacctata tcggtatagg
     9481 cattgttgca atgatttttt ttcatgtttt tgaaaatata ggtgcaagta tcggattact
     9541 acctctaact ggtattccac tcccctatat tagtcaaggt ggttctgcca ttcttagtaa
     9601 ttttttgggg ttgggattta tgttgtgttg tagtagatat actaacgaaa aaaaaggaac
     9661 acaaaaatga attcaaaata acactttatg aatagaatgt ctatttcaat aaaaattatg
     9721 ataccttaaa gttaat
//
LOCUS       DACDYJ010000089         9559 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDYJ010000089 DACDYJ010000000
VERSION     DACDYJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.090"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(33..1076)
                     /locus_tag="IWM51_002422"
     CDS             complement(33..1076)
                     /locus_tag="IWM51_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9971792.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
     gene            complement(1237..1920)
                     /locus_tag="IWM51_002423"
     CDS             complement(1237..1920)
                     /locus_tag="IWM51_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HAP9971793.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            2115..3044
                     /locus_tag="IWM51_002424"
     CDS             2115..3044
                     /locus_tag="IWM51_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP9971794.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            3104..3895
                     /gene="phnC"
                     /locus_tag="IWM51_002425"
     CDS             3104..3895
                     /gene="phnC"
                     /locus_tag="IWM51_002425"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAP9971795.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            3867..4646
                     /gene="phnE"
                     /locus_tag="IWM51_002426"
     CDS             3867..4646
                     /gene="phnE"
                     /locus_tag="IWM51_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAP9971796.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            4643..5425
                     /gene="phnE"
                     /locus_tag="IWM51_002427"
     CDS             4643..5425
                     /gene="phnE"
                     /locus_tag="IWM51_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAP9971797.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            5428..6231
                     /locus_tag="IWM51_002428"
     CDS             5428..6231
                     /locus_tag="IWM51_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9971798.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            6315..7862
                     /locus_tag="IWM51_002429"
     CDS             6315..7862
                     /locus_tag="IWM51_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP9971799.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(8159..8356)
                     /locus_tag="IWM51_002430"
     CDS             complement(8159..8356)
                     /locus_tag="IWM51_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971800.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(8432..8632)
                     /locus_tag="IWM51_002431"
     CDS             complement(8432..8632)
                     /locus_tag="IWM51_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9971801.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            complement(8752..>9559)
                     /locus_tag="IWM51_002432"
     CDS             complement(8752..>9559)
                     /locus_tag="IWM51_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9971802.1"
                     /translation="VLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLECY
                     FSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKE
                     TTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIVDRLL
                     SACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETYTTTLRTFKKFLPQIQ
                     NALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGKTFLTK"
BASE COUNT         2779 a         1898 c         1954 g         2928 t
ORIGIN      
        1 actgtagaca tcatccataa aatggacttt gtctacagtc tgaaatcacc actgatgatt
       61 ttctcttttg cgtctgaaac tgcctgatag gcttcctctg ataattgtcc ttttgtcaga
      121 tctacccctt cttctttcaa accaaaggtc aaatgcttgc cgccaggaaa tttatcgtta
      181 tgagaagttt ctgcaaattc tttgacttct gcaccgacat ttttctttgt agaagtcaac
      241 gtgaaattcc ctgtctcgcc gttctccatt ttataatccc cttcatccgc ttgatcgcga
      301 tctacaccaa tcacccatac ttttctgcta tagtcgtttt cattgcgggc ttttgcttct
      361 tggaacaccc cagctccagt acttcctgcc gcctgataaa gaatatcgac tccctctttg
      421 tacatagaag cagcaatagc ttttccttta tctagcgaat cataggatgc ggcatattgt
      481 acgttaacag taatttcctt gttcagtgct gtggcccctg ccttaacgcc agcttcaaat
      541 ccgctttgga aacgttggat cacgggccct tccattccac caataaagcc tacttgatca
      601 ctttttgtcg tatatgcggc agctagtcct gctaaatacg cactttcttg atctttaaag
      661 gttgctgatg agatattctt tttcccagta atctcaccat ctaccaaggc aaatttcaac
      721 tttggattat ctttagcagc attttccagt tcctctgctt gcaagtagct caagctaata
      781 atggtggtaa agccgtttct gattgcctga tcgatattgg tactgttatc ctctggattg
      841 ttcgtttcta tatacgtaaa atcttttttt gctgtcagct ttttctcttt tccccaagcc
      901 tctaatcctt cccagccagc ttgattgaag gagcggtcat caaccccgcc aaatccaaga
      961 accaaagcga ttttactttt ctctttttca ctttgcttct ccccctgaga ctgactacag
     1021 ccgactaacg ttactaatga caccaaaaac aacaatgaaa caagtatttt cttcaaaatt
     1081 tttctcccct ttttatgtgt cttgacacat tgaactttta aaagtatagc ataccaatat
     1141 atagaacact actcttcaac gaagattttc cgtttcccaa tttctccact atcaaaaaac
     1201 cagcaatcac ttctgactgc tgttttttta tagctgttat tctgctttta ctggactact
     1261 tatattctca gctaagtcct ctaaagcttc gatatcatga atcaagtaat aattcttttt
     1321 cttttcgata atattctcct cacgaaaatt cgtgattgtt cgtaaaagat gtcgataact
     1381 gacatttaag attgaggcgc aggtggttaa ccgaaatgag aaacgttcgt tttcgtgatg
     1441 ggtcaaaata aactttgcta aacgagtttc aacaggttct gttagtgagt tcgccaaatt
     1501 tatcccggaa tttaaacgat aacttagtaa ctggcaaata ttctgcaaaa agcgaacatc
     1561 tttttgaagc tgtgttcgat attgatttag cggaatgctg atacagatgc acttagtcac
     1621 tgctttaacg ctgcttttcg gcgataattc ccacaatgaa gaggcttcgc cgagtggtgt
     1681 agcgggcttt gcgtaatcga tgcaaatatt ttgtgtatca gaagaatacg agtacaccat
     1741 taccgtccca tccactaaaa aatacaaata atctgaggtt tttcccgttt gaatgaggtg
     1801 ctcttccttt tcaaaaaaaa gtaaagaagc cattgccaat atatctgaat ccatataatc
     1861 ctgcaggttg tgcttctcaa cgtaagcagt taattgcttc gtatcatgga tttttttcat
     1921 gaaatcgtca cctctaagaa gtttttcttt attatagctt agattgtttt tcggctcaat
     1981 acgaataatt atttataatt gtttgtaaat tgtatgtaaa agttgtgtaa aataggacgt
     2041 gcgtcacttc attgattttg attccctgct atgatttaga cgtattagaa attatatcca
     2101 acaggaggag aaaaatgcag ttcaaacagt tagcaaaagg gttcatcgga gtcatgttat
     2161 tgaccacttt ggcggcctgc ggttctggga atacaagtag cagctcagaa aaagagagca
     2221 aagaaaaatt agttgttcaa ttcgttccaa caaacaatga tggtagtatg gaggcaaaag
     2281 caaaaccatt tgctaagtat ttatcgaaaa agttagatcg tgaggtagaa gtgacgttag
     2341 ctactgatta ttcaacgatc gtcgaagcga tgtcttctgg tcaagtcgat gtcggaatta
     2401 tgccgcctgc agcctatgtt caagcaaagg atgctggagc tgcagatgca atcctgactt
     2461 ctcagctcgg cgattatgat caagaaactg gcttgccatt aaaggacaaa cttacgaaca
     2521 cctttaaagg agaagtattg gttcgtgccg atagcggatt gaataagtta actgatttaa
     2581 aaggaaaaaa tatcgcaacc ttaagtccta actctgctag cggctacatc tatcctgtcg
     2641 ctgaattgaa agatgctggg attgatccta ctgcagatac aaagatgacc actgtgaatg
     2701 atattccgag tgagattacc gcagtgttga acaagcaaat ggatgcggcg tttgtttttg
     2761 aaggcgcgcg aaatgttttt gcttcgaatt tcccagataa tgatttattt aaggatttaa
     2821 aagtcctgta cctgacaaaa ggcgatattc cgaatgacgc aatcgctgta cagcctgaca
     2881 tgtcaaaaga tttaaaagcg aaaattaaag agaccttctt aaatatgaag gatgataaag
     2941 atggcaaaga agcaatggct ttgtgggggc atcagggcta tgaagaagct gctgattctg
     3001 cctatgacac gatcagagac tacacgaaaa aagcagcaga ataaaacatc agtaagtgtc
     3061 agcaagcttt ttttgcttac acttaaaaaa atggagaagg ataatgatta tttttgacaa
     3121 tgtttcgaag gtctatccca atggcgttgt tggcttgaag gatattgact taacaattga
     3181 agatggagaa tttgtttcca tcatagggtt gagtggtgct ggaaagagca ctctgctgcg
     3241 cagtatcaat cgcttggcag aaaccaccaa aggagatatt cgcattaatg gaacctctat
     3301 tacaaaagcg aataaaaaag agctgcgaca gattcgccgc cagattggtc taatctcgca
     3361 aagcttcaat ctggtaaagc gtagtacggt tcagaaaaat gtgctatctg gacgtttggg
     3421 ctattactcc acttggaaaa gtattttagg tctttttacg aaggaagact accagcagac
     3481 aaaggaagca ctggaaaaag ttggtttgtc tgataaattg cagacacgca gtgatgagct
     3541 cagtggtggg caacaacagc gagtatccat tgcgcgagca ctcgtgcagc aagcccccat
     3601 tattttagcg gatgaacccg ttgcatcatt agatccaatc acgactcaaa aggttatgat
     3661 ggacctaaaa aatatcaatc aaacgatgga taaaacagtc attgtcaatc tgcattcagt
     3721 ccctctggct cgtgagtttt ctacgcgtgt gctcgcgtta aaagctgctc aggttgtctt
     3781 tgatggtcgt ccagacgact taaccgatga acgcttagag caaatttatg gcaatgcaat
     3841 ctttgaagaa aaggcaggcg aaatcgatga gcttgaaaga aaccgttcac tttaaatggt
     3901 ataaacacct gcttattctt ctgttcttaa ttgtttgttt tcaaggaagt gcctggatca
     3961 cgtcagctga tttttctcag gtttttcaaa actccaatca aatgactgtc tttttatcac
     4021 gcttcattca tccagacttt agctatttac ctaaattagt cgagcctatg gtgaaaacac
     4081 ttcaaatgtc tttgcttgga acggtcattg gcttaatttt agcgatccct gttagttttt
     4141 tagcgaccac tgtagtgaca gaaaaccgtc ttctttctac catctttcgt tttcttttag
     4201 gtttagtacg gaccatccct accctgcttt tagccgcgct ttttgttgct gtgtttggaa
     4261 ttggtgaagc gactggcgtg ttgactatcg ctgtctttac ttttggtatg gtgtcacaat
     4321 taatgtttca agcaattgaa accattgact ttggcccgat tgaagccgct gcggctgttg
     4381 gagcaaataa aatgcaaatc gctatttggt cgatcgctcc acaagttttg agtcagtttg
     4441 cgagctatgc tttttacgcg tttgaggtga atgttcgtgc ttctactgtc cttggatatg
     4501 tcggagctgg tggtattggc gttattctaa attcctcact tgcgcttttg aattatgaac
     4561 gagtttcaat cattattcta acgatcctag taactgtcgc gatcgttgat aaattaagtg
     4621 aacgtgtcag gaggtccctg ttatgatgct tcgtgaaatg aacagaagaa cccttttatc
     4681 attagttgct ttagtgattc tcgttatttt gtcggccgct actctggatt actctggatt
     4741 aagcaccttc tctccttcca tgggaatgga cgttttgaga gggttaggtc agcctgattg
     4801 gcagtttcta tatgatggca gcggcgagga tttgattagt ttgctattat taaccattgg
     4861 aatcgcctgt ttagggacaa tgatcgctac ggtattagcg attcccatta ccttgatcag
     4921 tgcggttaac ctctggcaag cacatccatg ggtaacaaaa attggcaagc ttatctgtaa
     4981 tgttttacga gcttttcctg aactagtttt tgcaattatt tttgtgaaag tcgtcggacc
     5041 tggccccttt gctggtgtta tggcgattgg cgtccatcag atcggaatgc ttggcaaact
     5101 ttttacagaa gaaatggaag cgatggatga gcgacttgtt gaagaaatgc atgcggtagg
     5161 agcaaatttt tggcaaacga ttttctttgt tcgcatcccc tatttgatgc cgatttattg
     5221 ttctttagca ttaaatcatt ttgaaatagc ggttcgtagt gcggctacct taggcttagt
     5281 tggcgctggc ggaatcggtg ctccattgat attcgcgatt caaactcggt cttggagcaa
     5341 agtgagtatt attttgattg gcgtagtcgt aaccgtgttt attttagatc aaataaccgg
     5401 aattataaga aagagattga gataagaatg aatatattgc atatatcaga tatacacttt
     5461 cgcagagagt atgaggcttg tgaagaaggc tataagggaa tgctggctac gatgcagaat
     5521 ccccttatcc ctctggagca atgcattcat catctcctgc agcaaacacc cttggacctt
     5581 gtgattatca gcggagattt aaccgaagat ggcgaaatag acgattatcg atacttgaag
     5641 acttggctgg aaaatgcgct aggagaaacg acgattcttg ttactctagg caatcacgat
     5701 atcaaagaac atttttgggt aggctggtgc gatgaagccg cctcttctgc cccgtacaac
     5761 cagatagtca aatatcctga gtttacagtg atttcctttg ataattcaag ctatggatac
     5821 gcagatggta tagtggacga tcagcaattt caatggctga aaaaagcctt agagcaagaa
     5881 aaagaccaac cgattttctt ggtcacccat catcatctac tgtctcacca aagcagtata
     5941 ccaaaatggc ctggtaccga acgtttcttg aagctaatcg caccttacga tattcgctgt
     6001 atcctaaacg gtcataccca tcacacattt actgatgaaa ttaatgggat cccctatttt
     6061 actgtatcga gtatgtcctt tgttggtgaa gatgaaggcg atggcttcgt acgctttgaa
     6121 gaacgtcatg ggtataacct ttatcatctc gatcaaggaa ggattgttcg ccagacaaca
     6181 gaaaacttta ttccaggcaa cattttaaaa acacttcaaa tgaaggatta aggcattata
     6241 aatcaaaaaa agagaccgaa cagctgaata cgcttcggtt tttctatttt gagtcagcta
     6301 aaggagcacg cagaatgaac attacgtttt tagagacaag tgatatgcat ggctatgttt
     6361 caccaacaga ctattccggc agaaccgatc tggccttagg aacggctaaa gtatcgtcga
     6421 aactgaagga attacgggca aaagcagatg gaccggtaat tacgatcgaa aatggtgatt
     6481 tcatccaagg gtctcctttg agttattatc ttgctaaaga atatggttca gccaaagagc
     6541 ttacagactt gattaaccaa atgagctatg atgttcaagt gttgggaaat catgagttta
     6601 actatggtgt ggattatctc gaggaagcga ttaagaatta tcaagcgcct atattaggag
     6661 ccaatatggt gaacaaaaat gaccaaccat actttggaaa cgcctataag attatcgaaa
     6721 aggtgggtgt aaaaattgct atcttgggat taacgacaca atacattcct cactgggaaa
     6781 agccagaaac cttagcagga attacgttta agagtatcgt cgaaacagca aaagaatatg
     6841 ttcctctatt aaggaaacaa gcagacttgg taatcgttag ttaccacggc ggatttgaaa
     6901 aagacttgtt aaccggtgag gacacagaag cgctaactgg agagaatgaa ggctacgagc
     6961 tattgcagca ggttgaaggc attgacgccc ttttcaccgg tcatcagcac cggaaaattg
     7021 ctgaaacggt tcatggagtt cccgttgttc aaccaggcta tcgtggcgag ttcatcggtg
     7081 aaattcgtct taccgtcgag aaaaaagagg ggcaagtggt tgttacagat caccaggttg
     7141 ctttacactc agttgaaaaa tcagagcctg atccagaaat tcttgcagcg atcgtaccgg
     7201 tagaagaaaa actagagaaa tggttggatc aacccttagg taaagtgaac ggaaatatgc
     7261 gtattaatga tccgatgcag gctcgtcttg aggaacatcc ctatgtagag tttattaatc
     7321 gtgtacaatt atttgccagc ggctcagaga tttctggaac tgcgttattc aataatgaag
     7381 gaaaaggctt tggtgagacg atcacgatgc gggacgtcat caccaactat atttatccga
     7441 atactctggc agtgattcaa ataaatggtg cagatttgag agcggcgttg gagcagaccg
     7501 caaattattt agctgtagaa aataaccaaa tcgtattcaa tccagccttt attcagccta
     7561 agccccagta ctacaattat gacatgtatg aagggattca gtacactatt gatatgaaac
     7621 agcctgttgg acaacggatt accgaacttc gttttaaagg tcaccccgtt cttccgcaac
     7681 aaatgctgga agtggtgaca aatcaatatc gggcaatcgg cggaggaaat tatgcgatgt
     7741 ttggtgcgga gaaaattgtc cgagaaatcc aaatcgacat gaccgaatta attgccgact
     7801 atttgaatat tcacccacgc atcgaagcaa aagtgaatca taattttcaa gtgattttgt
     7861 agtatataaa taaaaaattc aaaaaggtgt gagcagtttt ttgcccacac ctttgttatg
     7921 ccaaattgtt aggctgtctg atttccatcc agccattctt tagccagcga tctacctcat
     7981 caaaacaaaa aattttcctc tgatggttcc ttaaataaga ctattttgat cctttttcca
     8041 tgaataagat tttgcatcag acaatacttc atttctgaaa aagttgcggc accaatatag
     8101 cctaataccc ctttccgatc ttgattaaat acaaaaaata aatccgctgc tgcgatattt
     8161 aaaaaaaagt cgtcaagatt tctggatact ctttgtctag gatcggacat gagcgaggat
     8221 aatcaattaa ttcaatgcct tttttactga aatctttttc catatctcga tattcttctt
     8281 ggaaaacaat acttccagaa ataacagctt tcacaaaatt ggcctccatt tttttatttt
     8341 tcagacactc caccataccg gatacctctc aaagaaaaga catagcaaaa atcaatagca
     8401 gctatctctc cacggttttt tactggtaat tttatttttc ttttggaaat tgtatcagga
     8461 aggtcgttcc ttcatttttt cggctggtca cttcaattga accgtggtgc aggcgcacta
     8521 gttccttcac gattgataat ccaagacctg actcgcctat ctttgtattt ttacttgacg
     8581 gatcggcttt ataatagcga tcccaaatgt ttttttgttc ctcttcaatc atgccgatgc
     8641 cattgtcctc gacagtaata aatacaggct ctttgtcaaa tgatgttggt aaagagaaat
     8701 cttagaataa tttggacaga acagaaagaa gaaactctct ttctgttctg tttatttggt
     8761 taaaaatgtt ttgccatgac gaatcatgat ccgtagtttg aaattgtaaa agctgcgaaa
     8821 gccgtacgca ttgcgtttta atactttgat gtggttgttt aaacattcca atggtccatt
     8881 tgaatagctg tagtgaagcg cattttggat ctgtggcaga aactttttaa acgttcgtaa
     8941 agtggttgta taggtttcag gaagaccgct gacttctctg tttaaacagt cctcaaataa
     9001 tgactgatct cttttcttta ccgcataaag aatgtcttgg tataactgat agccttggcg
     9061 caggttggga caagcagata acagtcgatc gacaatctca ctttcagtca aataatcttt
     9121 aaaactggca cgccaaaccc ttttttcgta gtttaacttt gcttcttctt tttgtaggag
     9181 tttccaatag cgtttcaatt ttttccctaa ccctcgttga gagtgggaag ttcctttaag
     9241 ccgtgccgtg gtttctttca ctcgatgatt caagaaggtt ctgccaatat gttggacgat
     9301 gtggaaacgg tcgatgatca actgagcatt cgggaaaagc tttttcacca atgaaacata
     9361 aggcgcatac atatcaataa caatccattg aacggcttca cgagtcgcta atgaaaaccg
     9421 agaaaaatag cattctaaat aaggcaagcg tcgattttcg acaatatcca ataatgcatg
     9481 gctttgtccg tccatcatga taaaactcat tgcactggca acacttcgaa ccgatttgaa
     9541 ttcatcaaag cataagacc
//
LOCUS       DACDYJ010000090         9335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDYJ010000090 DACDYJ010000000
VERSION     DACDYJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.091"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..237
                     /locus_tag="IWM51_002433"
     CDS             <1..237
                     /locus_tag="IWM51_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAP9971803.1"
                     /translation="QKLSLGEVMDLVSNDGVYRYKVTRKFIVLEYFKLIDGVPEENSF
                     LSLPKKGEKPLLTLFTCVYTSQGKERYVVQGELQ"
     gene            290..2266
                     /locus_tag="IWM51_002434"
     CDS             290..2266
                     /locus_tag="IWM51_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9971804.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVINGTGAEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDIQLTYNMFVNDSAAIANPLTNSVDFVYSNNPFNQEEHHEKTKADAVTYGAKFLKA
                     DGGLFGTGIKATPLEGAEFAVKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            2279..3022
                     /locus_tag="IWM51_002435"
     CDS             2279..3022
                     /locus_tag="IWM51_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9971805.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPSVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIEEVQ
                     KDELQRNFVIYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEENRKQ
                     KLSWKRLLGSTVFLSVMIIGSLFVYKKKK"
     gene            3038..3811
                     /locus_tag="IWM51_002436"
     CDS             3038..3811
                     /locus_tag="IWM51_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971806.1"
                     /translation="MRNRKWLLVCAVALFSFTAYTQLTFADSQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKKLSYDQLKKVAEGETKTI
                     DDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLLTPVSNEKGEFLTDVWLY
                     PKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            4100..6211
                     /locus_tag="IWM51_002437"
     CDS             4100..6211
                     /locus_tag="IWM51_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9971807.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(6249..8306)
                     /locus_tag="IWM51_002438"
     CDS             complement(6249..8306)
                     /locus_tag="IWM51_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP9971808.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNHEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKASQAQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(8420..8632)
                     /locus_tag="IWM51_002439"
     CDS             complement(8420..8632)
                     /locus_tag="IWM51_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971809.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            8819..9286
                     /locus_tag="IWM51_002440"
     CDS             8819..9286
                     /locus_tag="IWM51_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971810.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIRKFI
                     NKPDSLFIVVPYEKDGVKKIVFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
BASE COUNT         3238 a         1472 c         1825 g         2800 t
ORIGIN      
        1 caaaagttgt cattagggga ggtgatggac ttagttagta acgatggcgt ctatcgttac
       61 aaggtaacgc gtaaatttat cgtacttgag tactttaaat taatcgatgg cgttcctgaa
      121 gagaactctt tcttgtcttt accaaagaaa ggagagaaac ccttattaac gttgtttact
      181 tgtgtttata cgtcacaagg aaaagaaaga tacgtggtac agggagagct acaataacaa
      241 ggttttaatc aacgcgagtc gttgaaatat gaaatgagga gagtaaaaaa tggcaataaa
      301 tcggtatgta aaaaaagctg gaatgcttgt tggaatgtta ggaatcattg caagctgctt
      361 tcaggggtat caagtacacg cagaagatgt cacgcaaaaa acgccaccag agaaggtcaa
      421 tatcacggtt cataaattaa tgtacgatca aggaacgcaa ttgaatgttg atattgacgg
      481 gatcaaaaac gatggttata cgcacgatca atatcctaca ggtgtaacga aatacaacaa
      541 ggcagattat ggtgatgttg agtttacgtt aggaaatatt acggatcaag tattaccgag
      601 ggaagattct gatctaacta acgcgaaagt ggacgaaatc gtgaaagacg tagaagacaa
      661 gggatctaac agtgagtacg tcaagaatgc aaccaacaaa attacttcag ctgtagatga
      721 aaatggtgag atcacttttg ctgatcaacc agcctatgtc aattccaaag ggaatgtata
      781 tgtggtttat gaaagtaaat ccgctgctgg attagtcaca caaaaagcaa aaccaatggt
      841 tgtgattgcg ccaatgactg ataatacagg ttcaggtttt ttaaaagaca ttcacttata
      901 tcctaaaaat attgtttcta aattgagttt tgaattaacg aaatttggtg acgatggcac
      961 agctcaaagc aaacaaacac ctttaaaagg ggcaaaattt gagttataca agggagaacc
     1021 tggaaaagga acaaaattag gagatcttgt atcggatgat caaggaaagc tgactgctac
     1081 tgatttgacg ttaggaaaat actattttgt ggaagttcct tcagaagttg tagtgggatc
     1141 agataaagaa ccaacagcag atcaatattt attaggcgca gacgctcgca acgatgcaca
     1201 caataaatta accttcgaaa ttacgaatga tggcgtgaca agtgacttaa aagcttccta
     1261 tgtaaattac aaagctcctg taatcgataa gacagtaatc aatggaacag gggccgaaca
     1321 ttctttccaa attggtgacg cagtgaatta tcaaggaacg attcatatcc ctacagatat
     1381 tgcaggtgga acagatggga ttacagtaaa tggtgtgaag agtgaaactt ctccttacag
     1441 cgtatttaag tggggagata cagcaggtaa aggactttct tatgtcgctg ctaaagcaaa
     1501 tatcaaagta acaaataaag atggatcagt ggtattaaaa gaaaatacag attataagat
     1561 ccaaaattca gaaaatggct ttgtgatcga ttttatcgtg aataatggcc aagtaagtga
     1621 tactgtcgca agtttacacg gacaagatat tcaactgact tataacatgt ttgtgaatga
     1681 tagtgctgca atcgcaaatc cattaacaaa tagcgtggat ttcgtttata gcaacaatcc
     1741 attcaatcag gaagaacacc atgaaaaaac gaaagccgac gctgtgactt atggcgcaaa
     1801 attcttaaaa gctgatggcg gactatttgg aacaggaatc aaagcaacac cgcttgaagg
     1861 cgcagaattt gctgttaaaa acgcagaagg aaaatattat ggtggattag ttgatacgga
     1921 taaagacggt gtcaaagaag cagtatgggt agacgatgta gcaaatgcag cgatcctaaa
     1981 atcagataaa gaaggtcact ttgaaatcac tggtttaact gaaggtgaat attcactgga
     2041 agaaacaaaa gcaccaactg gttaccaaaa attaacagaa gatattgcat ttaaagtaaa
     2101 caaaaactca tttaaagagg aaaatcgaat cactgttaaa aataatcaaa aagcagctat
     2161 tccattaaca ggtagtaatg gtttccaaac gtatgtattg atctcttgct tgttgctagg
     2221 agcaggggca ctttctgcag ttgtttactt taagaagaaa gcctagaagg atcagctaat
     2281 gattattcgt aagcccccaa aaagaagagt catgcacaaa ctgatgatat ttttttggtt
     2341 gctgtcggca gtagcagtct atttaattcc atctgtggaa acaaatgtag cgaaatttca
     2401 acaacagcaa agaattttag ccattgaaaa tcttgaaaaa acagattctt ctttggatgt
     2461 tgcgaaaata gagttaggcg atcctgttgg tgttcttaca attccaagta tttctttaaa
     2521 actaccaatt tatgacggaa cgtccgacaa aattttagaa aatggtgtag gtattacgga
     2581 aggaactggt gatattacag gaggaaacgg taaaaatcct ttaatcgcag gtcatagtgg
     2641 actttataaa gataatcttt ttgatgattt accttcagta aaaaaaggag aaaagtttta
     2701 tatcaaagtt gatggtgagc aacacgctta tcaaatcgat cggattgagg aagtgcaaaa
     2761 agacgaacta caaagaaact ttgtcattta cttagaacca aatcccaatg aagatcgagt
     2821 aacgctaatg acatgtacgc ctaaaggaat caacacacac cgtttcctag tgtatgggaa
     2881 acgagtaacc tttacaaaat cagaattaaa agatgaagaa aataggaaac aaaaattatc
     2941 ttggaaacgg cttttaggaa gtactgtttt tctttcagtc atgatcattg gttcgctatt
     3001 tgtttataaa aagaaaaaat aaaggagggt aaccaacatg agaaatagaa aatggctact
     3061 tgtttgtgca gtcgctcttt tttctttcac tgcctacaca cagttaacgt ttgcagacag
     3121 tcaagaaagc acggatagca cagaacaaac aatccaaacg tcagatagca gcacgattca
     3181 aagcagcgat gaaaccgtcg attcgtcaac agtggattct tcagaagaac cgaaaattgc
     3241 tgaagacgat caaatagtca aacagaccat tcacgttcaa aagattattt cttcagaaca
     3301 attagacaac caggggcaaa agcttgaaaa acaagaagga gtaaatggcg cgaagtttat
     3361 tgtctacgat atcacgaata tcttagaaga aatgtcacaa gatgataatg agaaaaatca
     3421 atctgtagaa ataattgagt caaaattgaa agatcgagca aaaaaacttt catacgacca
     3481 gttgaaaaaa gtggctgaag gagaaaccaa aacgattgat gatcaggaag gcgtgattga
     3541 attttccatt gaagtacctg caaacaaaaa acaagcatac tatatcgtta atgaatcaag
     3601 ccctgaaaac atttcaaaca gtgaagatat tgttttattg acacctgttt caaatgaaaa
     3661 aggtgaattt ttgacggatg tttggttata ccctaaatca gagaaatcag agccgaaaga
     3721 agaaattaaa aaagttgtgt caactggtgt aaagaagaac ttttttgaaa attgttggga
     3781 attttttgct caattatttt ttgataatta ggatgaaatg atgaaaataa taagaatatt
     3841 agtttttaat ctatcctttt tagccgcaac aatattgtgt tgcggtttaa ttttttctca
     3901 attatcaatt tatccaagat atcaacgatt gttcattcat tttctaaaca ttcaatgtgc
     3961 ttccatgcca ttagaggtga ttaaaaagct cttgatcaat ccaattttta tggttagttt
     4021 ggttcttctt acaggattgg taacgtgggt attaggaaaa aaagaatgaa aagcttacga
     4081 tcgtttaaaa aaatattaga tggagggcat tatggaaaat aaaattcatt gcactacggg
     4141 aagagtaatt actttagtgt tgtttttgtt ccttcttctt tttgcgaggg gaactgcagt
     4201 ccaggcctta gaaaaagttg atccgagtca aatcaaagaa aataataatc tggtgcttta
     4261 tcatgatagt gattcattga gaaatatcga agtaactggg atcaatggat ttaagatact
     4321 agatgagtat tcaactaaaa aacaaaaagt acttgcttgg ccaaaagtca aagatttgaa
     4381 aagtacgtca aaaatcgttg cccattatca aaaagtaggt acttatctag gaaaagaaat
     4441 tgattgtgac gtgacttttt ctaattttgt agcgcacgct ggggttaacc aagacggatt
     4501 agctaaaaca attaatggca aaaagtatta taattatttg ctctttgata aaaatattta
     4561 tcgaggattt ttcatgcagt caatcaatgt catgaatgca aaactagaat ttaagtatgc
     4621 aggaacaaaa acaaaagtag ctttgagtga accacagcca gaaattcaat caaaaatgag
     4681 atcgatgaga gcagctggta ttgatttaaa cgatacattt ctatccattt acagtttaaa
     4741 tggacagtat cagcaacgtg tgtcttggta taatgacaat aatgaatctg tcggtgaacg
     4801 taatattgat atgagagaat ttgttgggta tgaaaaaatg ggtagcttac cttattttgt
     4861 cacaacagat acagcatgtg cagaatacaa agctcctgcg ttatcaacaa acaatttaac
     4921 ttcaaaagta gtgggaggac gtgcagaaaa ggcttatagc tcgaatgatc atttcaccga
     4981 tgttgtagga gctgatactt atcacagaag tggtgtaacg tatacgcttc aaggcgcttc
     5041 cccaacattc atgattggcg caaatacgaa tagtatgatg tttagctttg atactgcatt
     5101 gctatggaca ccacaaccat cgaagcctac aaaagaagtg tttaacaaag ctaatactga
     5161 agaggcagca cacaatattg acaaaaaagt gattccacaa ggatcagatg tttactatca
     5221 tattcaccaa aagtttgatg cattaacagt caacacaatg aacaaataca aatcatttaa
     5281 aatcactgat acctttgaca gcaaaaattt tgatatggta tcggatggga aaaactatga
     5341 tggcgcattg tggtatgttg aagcgaatgg tacttggaaa aaagctgata caggaggaaa
     5401 gttaactgta tcaggtaata cagtcactta tactgcttct gacagctttt tagctaacgc
     5461 tgcaaattac ggtagggatt atattttgca gctccatatg agagcaaaaa atgatgcaaa
     5521 aggaattgca ccaaatgtgg ctactgtaac gtttaacaat gatccgaaca aaggaacaca
     5581 aaaaacaaat acagtagaaa actatttctc tcttaaacca aataaaaaag tggaacaaga
     5641 tgggaaagat gttgatggtc gaaatgatgg taaaaaagga acgcctacag ctcctttaaa
     5701 tgcaggatct gaagtacaat atctagtcac acaaaaatgg catacaaaag gtgtagacat
     5761 tgcctcagat cattacaagc aatttagtat tcaagatcca gtggaagatc gtttgacgta
     5821 taaagaaggt tctgcgcgag tgatagatat gagtacagga aaagatatta cttcagaagg
     5881 tacactaacc tatgatagca attcaagaac gctgaagtgg gaagcttccg ctgatttctt
     5941 aagtaaaaat cttttagatg gacgagaaat tcaactgata tttacagcta aaactccttt
     6001 gaatgaagaa ttggatatcg acaaccaagc aaaagtttca gtagaaagta ttgagaaagc
     6061 aactaatgta gtaacgattg gggtaaaacc taacttacct cagatcattg tgccaaaaac
     6121 tggatcgaat catttagtgc taactgttat tggaagtata ttatttctta ctttagcatc
     6181 attgggctat gtagttttaa aactcaaata aatataaaaa caaggctgag aatcagcctt
     6241 gttttctttt atttgaagat tggattcgca ctaataaata cattatctac gccaattcta
     6301 ccagcagagt caactacaaa attatgacct aatccgagaa tactttggtt attataaagt
     6361 aaatttgcat tatgggcagg gctatttttc catcctacca tggcatcgat agcaccatcg
     6421 tctgataaaa ggtcatcaat atacagccca gtttgactta tgttttctcc cttagcaccg
     6481 tagcagccta agctttccaa taaatcttgg aaagtaccac caccagggcg atagtgactg
     6541 aagctgactt gtaattcttt agctcgaata tctgcagcct gttgaacaat atcattgaat
     6601 tgcattgggg caacaccatt ttgttggcga taatcattta accaagcaag ttctttttga
     6661 cgcataaagt tacgtaattc gtctaatgaa atctttttat atttgaatgt gattgtttta
     6721 ttttgatcga cgttattaat ggatcgttgg ccgtttttat ccacggtaac ggtaggatcg
     6781 tcatttctgc acaagaaata attttgatca gtcatgtttt tccaaggagt agcaaagcta
     6841 tccccttcgt atactgtttt tgattcacta gagagaacag cgttggtatc agcatttaca
     6901 tgattaacag tcacattaaa tttacgagtg tagttgaaaa tataattttg actttcagtc
     6961 acattgttta aggttactga atttgttggt tctacccgat cgtctaaaac atatccttga
     7021 atagaaggag cagttaaagt gacactgtca ccgtgagtga cattgactat ctgttctttc
     7081 aaaatattaa ctcgtccttc gtttttgtag ttgttattgc acaacaaatt gatttgatgc
     7141 acttgattat aattgaatgt gattgttgta ttacctgtga ttgttttgct ttgagtatca
     7201 ttaccaatca ctttgtagct aaaattatct ttagcgcctt cgctatattt aaatgctttt
     7261 gccttagcag tgtaagtaga accagaagtt acttttgctt gatctgtagc aatcacttga
     7321 cctgtttgat tgtcaatatg gttaatagta accgtatatt cttttggtgt ttcaggtttg
     7381 tgttgcaacc ctttaaatgc agcatcaaga ctatttttag cattatttac ttgggcttga
     7441 gaagccttag aatcgtctag tactttttta gcattggata aagcagtctg aaaattattc
     7501 caagattctt ctgtaaaatc tcctttagca gttgatttta ctcggttgta taaagcttgt
     7561 aattcagctt tattcacagt aggaactggt tttttcttta atcctttgta agcagaatct
     7621 aatttatttt tggcactatc cacttgtttt tgagtcgctt ttgagtcatt taacactttc
     7681 ttagcattgt ttaaagcggt ttggaatgcg ttccaggtat tgtcggtata gtcacctttt
     7741 ttagttccct taacgctcgt ataaagagct tctaaagctg atttattcac tttgggttta
     7801 gaggtgtcag cgccatacca accgatacct tcgtatttcc agccatagtc tttggttaac
     7861 cattgtactt ctttccaact tgcagtgtag tgatgtgagc cagattttac accaggaagg
     7921 aaaaggcggt agatcggttt attatttttt tcggcagaac gccaggcagg cccttcataa
     7981 gtccagccgt aatttttagt caaccatttc acttcatccc aactagctgt gtaatggtga
     8041 tcacgaagac ctttattgta caaacgatag acaagatggc caccgctagt cggtgcttcc
     8101 caggcggggc cttcataatt tccccatttt atctttacta gatggtcacg ttcttttttg
     8161 ctagaggtgt aaaaatgttc atgattattt ggattgtaaa ggcgatacat gacgttagtt
     8221 gaagcagcgt caacagatcc agcaagacta agtgaggcag caaatgttcc aatagctaaa
     8281 acagtttttg gattaaataa tttcattttt tcaactccta aatttaatta aaaatgttta
     8341 catgattatt atatatcggc taaccgatat agtcaataaa aaataagaaa agcctgatat
     8401 aattttggct tttcttattt tatttgttag ttgaagtatt aatatcaacg atttcttcag
     8461 ccaatttatt taatttcata gcatttatga atctcatgtt gtctatacta gagcgtttgt
     8521 tggcaaggtc actgactgta gttaagggta cgccagattc tatggatata gaacgaactg
     8581 atttaccact atcaaacagc aaccattcga ttttcttttt atcagcgtac atttcaatgc
     8641 tccttcttgt tttttcttta ttatagctct tttttgtaat aaatcataat atcaagagaa
     8701 aaattttcta tagaaaggtg gatttttatg agtattcgtt ctccgtgtat ccaaatgagg
     8761 tttgttgcta ctttcgttat ttgatatact aaaaataaac aacgaaagaa ggaataaaat
     8821 gaagaaaaag caactatggt ttgtctctat gtttgcaata ttttttatta ttttaagtgg
     8881 atgttcaagt aataagaaat cggaagctaa agaagataat agtttattgc aaccaggaac
     8941 tgtttggaaa gaagaagtag gtggtcttat tcataattta aaaattatag atgatactac
     9001 ctgggaatat tctgaaagtg tatggcatcc agatcctgta caaataacag ttaagagaca
     9061 aaaagactat gaagggttag aaagatacaa aatagtagat tctgcaggta tcagaaaatt
     9121 tataaacaaa cccgattctt tatttatcgt tgttccttac gagaaagatg gcgtaaaaaa
     9181 aattgttttt cttggatcta gtaaggatga aaagcgaacg aaagaaaaat tgattcatga
     9241 tggatcacaa tcaaataaat acaaattaca aaagactagc gagtgattgc tagtcttttt
     9301 atttacccta aaggaggaat catagtggca aaagc
//
LOCUS       DACDYJ010000091         8681 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDYJ010000091 DACDYJ010000000
VERSION     DACDYJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.092"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            262..819
                     /locus_tag="IWM51_002441"
     CDS             262..819
                     /locus_tag="IWM51_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HAP9971811.1"
                     /translation="MSIITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLD
                     KSSFSGGQLFGTNPGGEFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGL
                     GACVIVINYDGLNMVYQEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWR
                     QAQGHAFTDDGTWIDPLPFLNSSKK"
     gene            835..1479
                     /locus_tag="IWM51_002442"
     CDS             835..1479
                     /locus_tag="IWM51_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971812.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIEQLKQQDGTKAKEEVTEAQSKTAEEFG
                     KLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            1490..2296
                     /locus_tag="IWM51_002443"
     CDS             1490..2296
                     /locus_tag="IWM51_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971813.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTHQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFAVKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            2318..2548
                     /locus_tag="IWM51_002444"
     CDS             2318..2548
                     /locus_tag="IWM51_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971814.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            3094..3369
                     /locus_tag="IWM51_002445"
     CDS             3094..3369
                     /locus_tag="IWM51_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971815.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            3411..3833
                     /locus_tag="IWM51_002446"
     CDS             3411..3833
                     /locus_tag="IWM51_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971816.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            3852..5444
                     /locus_tag="IWM51_002447"
     CDS             3852..5444
                     /locus_tag="IWM51_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971817.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            5456..5791
                     /locus_tag="IWM51_002448"
     CDS             5456..5791
                     /locus_tag="IWM51_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971818.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            5938..6132
                     /locus_tag="IWM51_002449"
     CDS             5938..6132
                     /locus_tag="IWM51_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971819.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            6164..8536
                     /locus_tag="IWM51_002450"
     CDS             6164..8536
                     /locus_tag="IWM51_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971820.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         3420 a         1334 c         1782 g         2145 t
ORIGIN      
        1 tattttggga ttacgtggtt tgatgggtgt ctgtttccta aaggaactgc gcgaggaatc
       61 ggtggtattg aaggtggctg gtacatgaag tttcccaact caaaagctgc cttctcctat
      121 tacggcttta tggtagccac acaatccaat tttaatgctt gtgtcggaaa taaaagccct
      181 ggcgcaagtt tattgatctt aggacgaggc ggttatgccg ctgcaggaat tactgaagat
      241 agtccgtatt atacaggttg tatgtcaatt atcacttcta ataaattaac cgaatatgat
      301 gaattcgcca tcaaacattg gggtgaaggt ggcaataaca atggcacaat tactggtgaa
      361 tggacgaatc cattccctgg cagcagttta gataaaagtt ccttcagcgg tggtcaatta
      421 tttggaacaa atccaggcgg tgaattccgt ccgaatggct tccatgatgg attagacttt
      481 ggttcagttg atcatccagg aagtgaaatt cacgcggtgc atggaggaaa agtcgtgtac
      541 gttggcaatc ctggtatttc aggtttaggt gcatgtgtga tcgtgattaa ttacgacggt
      601 ttgaatatgg tatatcaaga atttgcgaat agtacaggaa attctcgtgt gaaggttggc
      661 gatcaagtga aagttggtca agtaatcggg atacgagata cagcacactt gcatttaggt
      721 ttcactcgaa tggattggcg tcaagcacaa ggacacgcat tcacagatga tggtacatgg
      781 attgatccat taccattttt gaatagcagc aaaaaataga tcaggagaaa aaatatggaa
      841 aataaaactt taaaaatcgt cttaacagct gcaattagcg ttagtttaat cggcaatatc
      901 gcgttgtttt ggcaactgaa agaagaacaa gagacaaata cctttaaaat tgaagaagtg
      961 gctaaaaacg cgtctgagaa gctcaaaaaa aattcctctg aggcacaggc gaagatcgac
     1021 aagttaaatc aaaccatcaa tgagaaaaac caagaaatcg aacagctgaa gcaacaagat
     1081 ggcacaaaag caaaagaaga agtaactgaa gcacaaagta agacggcaga agagttcggt
     1141 aaattagcca ttgataaatc attaagcaga aatgaattaa cggaaaaact aaaagatgtc
     1201 gcaacccaag aagtgattga caaattaagt cctgcagacg atgatcacca acatgatgat
     1261 tacggaagtt actcctttac attaggtgac gtgcaaactt acgctcaaac aagcagtgta
     1321 aaagaggaac aaaattttgt cgtatttgtt gattatacaa tcggcaatcc tcaattcaaa
     1381 gatgttaaac cacaaaaaat aaaaggtgga ttaactgtca ctgaaaaaca ggtagatggt
     1441 caatggaaag tcactgactt tagttatttc actcgttagg aggaagaaaa tgagtaagaa
     1501 aaaaggagaa agacaacgtt tcaaaaaacg aaaaagttat cttagatggc tgggaatctt
     1561 tatgattctc ggatttttga ttcttgctag tccgatttac acgcatcaga aggtagacat
     1621 accagatgaa gtaagcacga acgcattttt aaacacgaag gaactaacga tggtttcaaa
     1681 agaaaaagga gcgaaccagc aattagtttt tgaatttgca atcgacgatg aagatcaaag
     1741 cttattaaaa gaactggcta atcttaatta tcgcgtagaa gtaaagacag ctaaagggga
     1801 ttatcaagca ataaaagcca aagtgattaa ggtgtctgat gattattttg cagtgaagct
     1861 atcgcatgta ccagaagaat atattgctat gcgattaacc attattcctg aaaaaattga
     1921 tccaaaggtg gatatgcaag aaccacaaga tctgatttat tacgttcacg aagataaggt
     1981 taaagatcaa gtgaaagatg aagattatga acaacatgcg atcaattaca aagtgaaagg
     2041 atacaagaaa gaacaaaaag cagctcaaaa acaaatggaa tctttccaag caaccattga
     2101 tttgaatgaa gaattagtga agaagctaaa agatcaattg ccttatcaag tcgcagatga
     2161 tcaggaaaat actgaaaaca agattaatag ctaccagcaa gaaattgaaa catcgaaaac
     2221 gcaaatgaaa gatcaagaag agatcattca aaaacttcaa gaaaaaattg atcgtatgac
     2281 aaaagaaaag atttagaagg acaggtggag tgaaaaaatg aagcgaacaa acttggctga
     2341 caaagatact ttcgctgagc agaaagtatt gattaaagcc tatctcaaag ttgtaaaagc
     2401 taaagaaata tgtcgagaag tgaatttatc taatacaaca atatctgagt tgaagaatgg
     2461 tcgaaagaat ttagatactt taaaattcag atcatttgaa gctctatatg tagctgctaa
     2521 aaaatatttt acagctgata acaaataaaa aacaggagta ttttatgtga aaaaagtata
     2581 taaaaaatgc ttatgaatca gctatatcac cttaaccatt tgttaatgag atgggagaaa
     2641 acattaacca cgattctaac gcaaacatca agcacataaa acatttaaca ctcctccttt
     2701 ctggctgaag atttcaacca aacaggaaga cagcgtatca attatagcat acactttttc
     2761 ttaaattcat ttttcatatg acaaaatatt tccttctaat cgacaaagtt agaaggattt
     2821 tttattgtct aaatacacaa ataaccttaa tttataaaga gcataaggaa acgtgatatc
     2881 actttctcac gaaaggtgtt aggtaggctg tgcctaccac gtttctaaga aagataaatg
     2941 atatcaaaat tttggatttg tgtgtagatt tgtgggtagg atgaaagcga atgtgggcag
     3001 taaaataagc gtttgtgtgt agcaaaatca aaaaatgtgt agggatatgt gtagctacac
     3061 atatccctac acataataaa tttggaggaa aatatggact tattaattag aaatattgaa
     3121 gaaaagtata ttaataaaat tgacaaacgt tgtgaagaac ttacggtcaa gactggcaaa
     3181 aaatggagtc gaaatcaata cctaaaagtt ttgatcgaaa atgattttga tcacgctctt
     3241 ctgaattata aaaaagacca atttgatcgg ttgttagaaa aatttgtgga tattcaaact
     3301 tataatacga aaactttaga agaatatatt gcaacaaata atcaattaat tggtttgtta
     3361 attgaataat aaaaatagta ttgtgaattt ataaaaggag gaaatcaatt ttggcaaaag
     3421 caacaagaaa acatgtatat ctttacgata tgcatatcga agtacttgat gaaataattt
     3481 ctactcatga agatatatct aattatagtg aagcagtcag acatttgtgt atgatgtatg
     3541 atccaaaaat cgaagaaaaa ataaatccaa ttcaaattaa attgaatgca atgggaaaag
     3601 aaatttctat tttaaccgaa atggtttcag agtttgcgga tcttcatttg aaagatacag
     3661 gaatactaat tggtacaaat tcaactgttt atcaagaagc agaagaacgt gtggaaggaa
     3721 aaattaaatc caatcaaacc aaaaaatatt cttctaagtt aaagaaacca gtgaatgaat
     3781 ctgttcatac cagagtagat ccatttgatc caacgaagat tctaaaaaga taaaagtaga
     3841 aggagaacgt gatgaataaa tcgcctgcag ttattcttac ttgtcaattt accttgccca
     3901 ataaaaaatc tttttcaaca tacattgatt atatgacaag agaaaaagca ctggaagaaa
     3961 tggaacgacg tacgcccgaa gaagaacagg aactgcaacg aatcaagaat gctttagagg
     4021 atttttatat tcctgaagga gaaacttttt cggaaagtaa gacggataaa gaattcagtg
     4081 aaacgtcgaa tgaagctcaa tcgttgatgg aacatggaat caattttgac aagttagagg
     4141 aacaagattt taccaaatat ctttcttata tgacccgtca ctatgcgtta gaacaaaaga
     4201 aagaactgac gcaaagggaa caaaaagaat accaacggtt gaaccaagca atgaaaaaat
     4261 atcaaacaga gccacataaa aaagcttcag gaaatcttcc tggagttttt tctatggctt
     4321 cagacgaagt gaaaggaagt gatctgaagg agatcaaaca aatcttccaa agaggacaac
     4381 aaaaaggctc aatcatttat caagatgtta tctctcacga caattcgtat cttgaaaaaa
     4441 tgggcttgta cgatcccaaa acagatacct tagatgaaga aggtttgaaa gcagctggaa
     4501 gaaaaatgat ggagacatta ttcgagaaag aacaattgaa tgaaacaggt tactggatgg
     4561 caacgattca ccgtaacact aagcatatcc atattcattt tgcagttgtt gaaaaagaaa
     4621 atacgcgtaa accgtatact gagattgaaa atggtgtgag ttatatcgta ccgaagggga
     4681 agagaagaca agcaacatta gacgcaatga aaaccaatta tgtacatgag ttagaacgtt
     4741 tctcttatga aaaggatcga atgatcggta tggagaaacg aaatttattg acacgaaaaa
     4801 gtgatttgcg taacacctta acaaaagtag taaaagaacc agcacgttac gatcgacacg
     4861 ccatgaactt attaaatgaa gtatataaaa ccctacctga aaaacgttcg gaatggaatt
     4921 atggagatga aaatcgaacc aagctttcac catttacaag gggaaaactt gatgagttaa
     4981 ccaaatatgt tttaacaaaa gaacctggat atcaagaata tatacagatt tcaaaagcat
     5041 tgaaagaaga agccaaaagc ctttatgggg aaagcaaaag agaaacaaag aatgcagaaa
     5101 aaaacgccct ctttgattta aaaaagcgca caggaaatgc gattttaaca gaactgaaga
     5161 gacaggatag tcaaataaaa ccattaatag aaagtgtcaa tcagatgcaa tctatttaca
     5221 atgaacgatc ctttcgaaca ctcaaacagt tgccaacaaa tgaatacaag tatagttatc
     5281 aagaattcgt aaagcgtagg aaacaattcc aacaaagagt tgtcacgcag cgaagcttgc
     5341 gtcaattaaa tcgaacaatt aatgcacatg aagaaaaata ccacgcaagt aaagcatatg
     5401 aagagcaaca gcgaagaatc caaatggaac aagaaagaca ataggaggaa gagaaatgaa
     5461 acaatattta gatcaatgga aagtcataga aggatcacta aaagaagaac gaatcgaaca
     5521 acttcctgat tgtttagaaa aagagcattt gtttcagata agcgaaatgt tgagaaacga
     5581 acagtttgat ccaaatcaat ttttagttgt tgagtaccca gctactggtg tgtattgttg
     5641 taatcatgtg aagggtgaaa aatattttat tattcaagaa tacgaaggta agttagcgcc
     5701 ttattatact acttgggaaa taaatgaaga gggaatcaat aatttcccat gtgaaagcat
     5761 tgaagagtct atttctctca cggaatgtta aataaataga gacgtaaaag agtcctgaac
     5821 gtgattgtcc aggactcttt ttcaatgaat tgttttccgc caattcactg agtggtgcga
     5881 ttcaatttta ttatatcaaa agaaagtgag attccaaagt gaagggagga agaagaaatg
     5941 actcagacaa cgattggtgt tgtggttgaa aaaagcaggg ataatctcgt ttttgtgaca
     6001 gaaattcaaa caggtagagc ttttgtagtc acagacaaag ctgcaaaagc gtatcaaaat
     6061 ggcaatattt taacgttaaa tctgactacg aagatgttcg ttgatgcagt agaaaattat
     6121 ccatttgttt agacaaacga tattttacaa ggaggaaacg gacatggcag aagaaaaaga
     6181 accactagat aatagtcgtt tgggaaagtc caaaagaaaa ttagtacggt tgcaaaacga
     6241 attaaataat caatttcaaa agctatttga tcaccaaaaa ttgacaaatg ggcaaccaat
     6301 gaatgacaaa cgaaatggtc gttcatggtt taaccagcaa gaaaaaatag aaaataaaat
     6361 acaaagtttg agagaagaga tcaagcaaca agaaaaacag gtagaaaaac ttgaaagaca
     6421 agaagagcta aaggaaatgg gttataacaa gtacggtggc ctggacatga cgatcgaaaa
     6481 tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa ggtgaatcga ctttttcagc
     6541 tgccacgatc cgaaaatacc aacgaaaatt ggaaacatta gaacagctga aagaaaaatc
     6601 agagaaagga aaagaaaatc ttcttcctga agtacaagcg atcattgaca gcggacgagt
     6661 gacgcaatgg aaaaagaatc caactatcta ttttctgaaa ggctatcgaa aagtcgcttt
     6721 agaactcaat gaaaacgggg gatttgagga atctaaaaaa taccaagcca cgtcggaaga
     6781 agaaaaggca gctgttcgtg aattacttga agaagctaca cctactgatt ctgtgaagga
     6841 gaaagaaacc gatcatttct atcgagtaga attcaacgaa aatgatccag caaaagggtt
     6901 gaaaagttat ggtggggaga tcgtaacact agaattgatc gatcaactca aaacactgga
     6961 taaccaatac gccgcagaag caggctatta taaattctat tttgaagaag tgaagaatgg
     7021 agaagtagtc gatcagatac gaatggatct aggggatggc gtcaatagta atcaccccat
     7081 ctatttagag ttagctagat tgtgcgaaca atcacctgaa aaggaacaag aacaaacagt
     7141 caaaaatgaa cctatttcaa aagtacaaaa cgagattaaa gagacaaaaa caaaacaaaa
     7201 gggttatcaa aaacgaacgg cagaagagat taagcaagaa gtcaaatcac tttcccaaca
     7261 ggcgcttgat gcggtgaaaa agcatactca ttcacctgaa gacgtcaaag aacttttgaa
     7321 ttttatgtct cgttttccag aacgatcctt tagaaatcaa ttattgatcg aggaacaatt
     7381 tccaggcgca acggcttgtt taggtcgtgc cgcgttgaaa aaagaaggaa tctatatcaa
     7441 aaaaggagaa caagggaaca agatctttgt cagaaaaacg gtcaaaggat tttatgagaa
     7501 aggacgtggc tttgttcgag aaacagaggc tacaccagaa gataaaaaac gaatcgaaag
     7561 tggtcaaatc gaagtgatca aaaaacctta ctacacgatt gaaaaagtgt ttgatatcac
     7621 acaaacacaa ttgaaacctg aagactatcc gaaaatcttc ccgaaccgcg tctttgattt
     7681 tcaattggat caaacagggc agacagaact tcaagttggg attcaagcgg tagccaaaaa
     7741 tattggaatt caaattcgag atatgacaga aagtgaagtc tatcaaagag aattaggaca
     7801 agcacgtggg gcgtacgttc gtaacgaatt tactggcgaa gaagaaatcg tcatgaatac
     7861 tcgaaatact cctacgcagc atttagccac aagtattcac gaattagccc atgctagaat
     7921 gcacaaattt tcagaattag atactgcaac aaaagaacta caggcagaaa tgacctccta
     7981 tattgtgtgc aagcattttg ggatggacac ctcggagaaa gctattcctt atattgcgac
     8041 atggacgaag aatggtcaga cgttggatga taaggaagct gcggaacgtg ggaaaatcat
     8101 gaatgatgtc agtcgtgtcg caaatgaatt cattcaaacc atttctaatg aaatcaatca
     8161 atacagagaa agagaaccag agatccagcc agcagaagtg aagcaggagc ataaaaatac
     8221 tgtagaaacg gcagaacaaa agaatattgt gcccgttggc tcactttgga tcgacaagaa
     8281 agacgggaaa gtcctggaag aagacacggg ccgaacgtta catttgaagg atgcggcgtt
     8341 tattgaagat ccgcagcata atcaagtgat tttaaaagga aaaagttatg gaaaagagat
     8401 ccaaacggcg ttaccttcag aagaatttgc gctaggaaat ccattccgat tttcacttga
     8461 gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat tatgaacaac aattgtcaca
     8521 aaaagaacgc gtataaaaca agcaagaaag actatctgaa aaatggatag tcttttttct
     8581 tttgggaggt ttgaaaatga cagagaaaaa aatgagtctc attgatcgat gcaagcaaat
     8641 ggatattgtg gattttgcac gaaacaatgg tttagcggtg g
//
LOCUS       DACDYJ010000092         8586 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDYJ010000092 DACDYJ010000000
VERSION     DACDYJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.093"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(45..1511)
                     /locus_tag="IWM51_002451"
     CDS             complement(45..1511)
                     /locus_tag="IWM51_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP9971821.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="IWM51_002452"
     CDS             1898..2494
                     /locus_tag="IWM51_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9971822.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="IWM51_002453"
     CDS             complement(2552..3034)
                     /locus_tag="IWM51_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971823.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="IWM51_002454"
     CDS             complement(3140..3337)
                     /locus_tag="IWM51_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971824.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="IWM51_002455"
     CDS             3632..4390
                     /locus_tag="IWM51_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP9971825.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="IWM51_002456"
     CDS             4450..4878
                     /locus_tag="IWM51_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971826.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="IWM51_002457"
     tRNA            4924..4996
                     /locus_tag="IWM51_002457"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="IWM51_002458"
     CDS             complement(5146..5517)
                     /locus_tag="IWM51_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9971827.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="IWM51_002459"
     CDS             5732..5917
                     /locus_tag="IWM51_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971828.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="IWM51_002460"
     CDS             complement(6000..6188)
                     /locus_tag="IWM51_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971829.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="IWM51_002461"
     CDS             complement(6212..6475)
                     /locus_tag="IWM51_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971830.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="IWM51_002462"
     CDS             6633..6890
                     /locus_tag="IWM51_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP9971831.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6961..7152)
                     /locus_tag="IWM51_002463"
     CDS             complement(6961..7152)
                     /locus_tag="IWM51_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971832.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7293..7613)
                     /locus_tag="IWM51_002464"
     CDS             complement(7293..7613)
                     /locus_tag="IWM51_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971833.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7636..8070)
                     /locus_tag="IWM51_002465"
     CDS             complement(7636..8070)
                     /locus_tag="IWM51_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9971834.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8405..>8586
                     /locus_tag="IWM51_002466"
     CDS             8405..>8586
                     /locus_tag="IWM51_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9971835.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2718 a         1694 c         1362 g         2812 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt
     6961 ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc
     7021 tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag
     7081 gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt
     7141 tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta
     7201 tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat
     7261 cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct
     7321 tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat
     7381 aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca
     7441 gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct
     7501 ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt
     7561 gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat
     7621 tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata
     7681 agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac
     7741 gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc
     7801 agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt
     7861 cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc
     7921 actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc
     7981 ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc
     8041 taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa
     8101 catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc
     8161 aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat
     8221 tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt
     8281 acaaaaaacg tggatttgtc ataaattgtc atgcttcttt tacctatgaa atattttcac
     8341 gtgttaaagt attcctcgtt gaacacaaca aataaaactt tttaacgaat aaaggagatt
     8401 tttcatgaca tcacttaaaa ctttactttt tggaacaact ttggctgccg gcgctgcatt
     8461 cttcatggga acgactgctc atgcggacga agcttatacc gttcaatcag gtgatactct
     8521 atcaacgatt tctcaaaaat acgttggtga taactccttg atcaatgcca ttgcagaatc
     8581 aaattc
//
LOCUS       DACDYJ010000093         8485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDYJ010000093 DACDYJ010000000
VERSION     DACDYJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.094"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            168..362
                     /locus_tag="IWM51_002467"
     CDS             168..362
                     /locus_tag="IWM51_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971836.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IWM51_002468"
     CDS             631..930
                     /locus_tag="IWM51_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971837.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IWM51_002469"
     CDS             complement(1027..1461)
                     /locus_tag="IWM51_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9971838.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IWM51_002470"
     CDS             complement(1693..2403)
                     /locus_tag="IWM51_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9971839.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IWM51_002471"
     CDS             complement(2393..3253)
                     /locus_tag="IWM51_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP9971840.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IWM51_002472"
     CDS             complement(3258..3896)
                     /locus_tag="IWM51_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP9971841.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IWM51_002473"
     CDS             complement(3911..4210)
                     /locus_tag="IWM51_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9971842.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IWM51_002474"
     CDS             complement(4253..5737)
                     /locus_tag="IWM51_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9971843.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IWM51_002475"
     CDS             complement(5757..6218)
                     /locus_tag="IWM51_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9971844.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IWM51_002476"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IWM51_002476"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP9971845.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IWM51_002477"
     CDS             7521..8291
                     /locus_tag="IWM51_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9971846.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
BASE COUNT         2658 a         1748 c         1352 g         2727 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgcccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatacttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaa
//
LOCUS       DACDYJ010000094         8229 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDYJ010000094 DACDYJ010000000
VERSION     DACDYJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.095"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="IWM51_002478"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="IWM51_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP9971847.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="IWM51_002479"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="IWM51_002479"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP9971848.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="IWM51_002480"
     CDS             2591..3376
                     /locus_tag="IWM51_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9971849.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="IWM51_002481"
     CDS             3389..4240
                     /locus_tag="IWM51_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP9971850.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="IWM51_002482"
     CDS             complement(4364..4816)
                     /locus_tag="IWM51_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9971851.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="IWM51_002483"
     CDS             complement(5281..5649)
                     /locus_tag="IWM51_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP9971852.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="IWM51_002484"
     CDS             5799..6281
                     /locus_tag="IWM51_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP9971853.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="IWM51_002485"
     CDS             6318..7328
                     /locus_tag="IWM51_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP9971854.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="IWM51_002486"
     CDS             complement(7443..8156)
                     /locus_tag="IWM51_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971855.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2441 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtct
//
LOCUS       DACDYJ010000095         7824 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDYJ010000095 DACDYJ010000000
VERSION     DACDYJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.096"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1127)
                     /locus_tag="IWM51_002487"
     CDS             complement(<1..1127)
                     /locus_tag="IWM51_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9971856.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIK"
     gene            complement(1163..1873)
                     /locus_tag="IWM51_002488"
     CDS             complement(1163..1873)
                     /locus_tag="IWM51_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9971857.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            complement(1941..2756)
                     /locus_tag="IWM51_002489"
     CDS             complement(1941..2756)
                     /locus_tag="IWM51_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP9971858.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            complement(2767..3735)
                     /locus_tag="IWM51_002490"
     CDS             complement(2767..3735)
                     /locus_tag="IWM51_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9971859.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            complement(4469..5671)
                     /locus_tag="IWM51_002491"
     CDS             complement(4469..5671)
                     /locus_tag="IWM51_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HAP9971860.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVT
                     EYIKNYFN"
     gene            5692..6465
                     /locus_tag="IWM51_002492"
                     /pseudo
     CDS             5692..6465
                     /locus_tag="IWM51_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            6644..>7824
                     /locus_tag="IWM51_002493"
     CDS             6644..>7824
                     /locus_tag="IWM51_002493"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9971861.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQ
                     YRQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQ
                     DLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLEN
                     L"
BASE COUNT         2401 a         1591 c         1235 g         2597 t
ORIGIN      
        1 ttttttataa cagttgacat gtaagcctct tctaacgttt gattcaagcc gagaatttgt
       61 ggatatacct ctgctactac ttgtccttcg ttcatttttt ctgctgtctt ttgagtaacc
      121 atttccaaat cactaatcga attaaaaacg ccttcaggta accgctttcc aaattcgaca
      181 agaattgacg cagctttata acccgcttta tctccttccg ttgtcaaaca gcaaggaatc
      241 tctacttcat tgaatgcagc aacttctgct ttaattgcct catagtcaat ctcgggtgaa
      301 gctactcctg actgataaac accgtaaata tctccacttc tatccgcaac aacaccatct
      361 accatgatct tcactccacc aaagcggacc ttttcaggga aaactttttc cttatactca
      421 tatagaagtt catgctgtag aggttcttgg actgcctgca catataggtt actaagtatt
      481 ttcctctgag gtatattaga aagaaattct gcgtcatcaa aaacgatttc tttcattgat
      541 ataacacctt ttgaaagcag taatgcttca aattcagcgt aaacctctgc taaaagcttt
      601 ttgtttgcta gcatttcctt aattaataat actcgacttt cagcactggt ttctttttct
      661 gtaaaaccat aagtctgctt agcaatagca ttcatccaac aatggctgcg atttttgtcg
      721 atcgctgtga ttggaccaaa gtggtttatt tcattcaata gctcttctga tgggtcagtt
      781 ttccacaggg cagcatccca gccatgagca taaattggtt catctgcttt tttatggttc
      841 attacagctt taatacgctc tacagcctcg ttagctgaag atgtatcgct taaatctatg
      901 ccagcattca tagccaaata accactgaaa aatacatgat tatcatgaat tgctggcata
      961 acgaattgat catcataagc aattaattcc gtgttttcat ttaatagatc actgtaattc
     1021 cagttgtttt ccaccttttc aatgatccct tcattgatta caatataacc tttgaaaggt
     1081 ggctgctctc catcaaaaat catttttgac cgcaatattt gcttcataaa taaaatctac
     1141 tccttgaaat caaaacagca aatcataagt aaaaacgata tacttctccc aggtaatatt
     1201 gattacctac atgaattggt atgttgtttt gatcgttgat taaagtatcc ataaagaaaa
     1261 agggcgtccc aacagctact tccattattt tggcaatttg gtcatcagct gtgacaattt
     1321 caagtgcagt ttccccagga ttgatggcta aggtaccttg gactttaagt acttcataca
     1381 aagaatcagt cagctcttcc tccattaaga aactatatct ttcataagaa aaataattgt
     1441 tttctaacat aatggggatg ttatccgcca ttcgctttct ctggatatat aaaacatcat
     1501 caccttgagc gatatcgagt ttttttgcca tcccttcagt tgctggaatg atcttgcgtt
     1561 ccaaaacaat gctagtcggt gtaaaaccat tagatttaca actctcactg aaacccataa
     1621 catattcaat tttacgaaaa actttatgcc ggttgacaaa agttcctttc ccttgttttt
     1681 taagtaggta cccttcttct accagttctt ctactgcttt tctcactgtg attcggctga
     1741 cctcataact ttctatcagc tctgattcag cgggaatttt cgtattctcg ggatactccc
     1801 ctgtattgat tttatttttt aatatttctt ttaattgtaa gtacaatggt aattgtgcat
     1861 ttgtatccag cattttttta acctccactt taccgggatt tctttcttta ggttatcaaa
     1921 tgcaggaatt taaaacaagc tcaatcgtaa tgaataccaa agccgaacga accgtcaacc
     1981 agacaagact tcccagcaaa ttcattcccc tttttcatcg cggtgactat atcgtcccct
     2041 tcgagaaaat aaagtaggaa agctgttaag aaagaatctc ccgctgccat tgtatcaact
     2101 ggttcttcta gcacggggtt cacttcataa aactttgctc catcataacc aatagccgtt
     2161 ttttctcctc gggttgcaat caatacatca tttcgctcac aaaaatgctt catcaaaaac
     2221 tgctttactt cttcatcgga aaagtcttct atagaaaaga agccaaaatc aactttatca
     2281 attgcccgct gaacctcttc ttccgtaaag tcgtcagaaa aatcataaga aatctttcca
     2341 gaaacatttt gtaactcttc taatagatga ttgacatggc tatataatcc catatgaatc
     2401 aattcaaact ggtttaaata ttctctgtct tcttcagtaa gctgaatcgg gtattcacga
     2461 gtaacacctc ccgaattgct gccaaggaaa atccggtcgc cctcatcaat cttcactctg
     2521 gcacagccat tctcgccttt ttcaaagcga gagtgactgt tgtccagtcc tatttctttt
     2581 attgctgata aaacatgagc tgcctcctca tctgagccaa aaactcccat aaagctactt
     2641 tcatgtccca gtttttttgc aaaaactgca aaattcaatg aatttcctcc aggatacata
     2701 attcccagat tttcatattt atcgacgacg ttatcaccta agcctaatac cttcatttta
     2761 atcctcctag tactccactt tccccatata gcgacgaaca tctaaatcat gattgcgtgc
     2821 atctgctaaa gctgaacgat attctgaaag aatactgtag aataaaatgg gattgaagaa
     2881 ttcagaaact tcatctgata tcatatccaa tcctagttcc tttgcatcaa cgatttctac
     2941 tctttctgca tatttatcaa ggaatcgttt tgcccgttca tctaaagcac gagtgcgtcc
     3001 aatgctcatc aagagaataa aattcgtatc tttgtctgtt acttcaaaag gtccatggaa
     3061 gaattctcca gaatgaacag aagttgaatc aagccattgc atttccatca aagagcaaat
     3121 tgaaaaacca taagcatgtc catatgatgg tccactacct aagatataaa acattgtttc
     3181 atctttgtac ttatcagcaa acactttaat acgatcctgc acctgcttaa tgccattttc
     3241 aattacggta tcgataacct tcatgccttc tctgaattta ccgtaatgtt caaatgtttc
     3301 ggtaacattg accaaagata cagttaaatc aagaatgatg gccataggat tattgactat
     3361 ttttgcatca tctccccatt cgtaaaggaa actatgatca gaaaccttta ataaatccgc
     3421 agttttatta tgagtcagac caacagtcac agctcctcta gcttgagcga cttctcctgc
     3481 ttttaccgtt tctgctgtat ttcctccatg agaacataga atgacaacag atttatcatc
     3541 taattgcttt ggggttacat ggttaaattc gttcgctgta tataagcctg taatacactt
     3601 cgatgcttcg gctttaagaa aataatctga gacatacata tctaccagcg aaccgccaca
     3661 agccaccaaa taaattccag aaatctctcc atcatttttt tctacaattg cgttaaccgt
     3721 ttttgctaca tccatcaaca tttcctcctt aaaattttaa aaatgttaat tttatccaaa
     3781 ataaaagtaa ctgattaaat gtaaagatat tacataacgt catataacgt catgtgacat
     3841 aattgcattg tatccgcttt ctttgtgttt gtcaatagga aaaatttatt tctctaaaaa
     3901 atataaataa tctgcagtga aacttgagta catatactaa tttcgaaaaa tgcctatgat
     3961 ttaaataatt atggtgctgg gatgaaggtt tataagtttg tcttgatttg tttagaagaa
     4021 atcgtttttg cacacagttg aattaggaaa ctactggagt ggaaaataaa gtgatttgac
     4081 gggtagatgg gtgcacttaa atttgaaaat ggcttaaaat gctttatttt gaattgcaga
     4141 atttctttta gtacgcaatt tatggtctta aaattgatta aaaagtgata cattgatttt
     4201 aatacaattt atgatacaaa aaatgacctc ctttcttaaa taaaagaggt cattttgttt
     4261 tgtctcataa tcgattgttt ttgatacagt agttatattc agaaaaaata aagactcttc
     4321 tgagtttata atcctttgga ttatatacgg atacagatgt agacataaaa caagctcaat
     4381 tttcaaaaat ggcattactc acctttttgg tgaattatta aactaaaaaa gatgaatttt
     4441 tttatgtact ataagatgta ggtgtactct agttaaagta attctttatg tactctgtaa
     4501 catcgaatac atcttctaat aaaaatgcac gcaataatct gtcaattagc atatttacct
     4561 tgattggtat taccatttta cctaaatttt tctcgatata tccactgaaa tgacctacgt
     4621 taacacttga ttttaattta tcaaatcgac tatattcatt aatgtttacc tcggttaagc
     4681 gtaaatccaa catctcacgt aataatgcct cattaatatt ggttgaatcc actaaatctt
     4741 ggataaaatc gtcctcttca ctaagctgat attcattgag gtaatcagta aattcccatc
     4801 cttctttgat aactaattcc ccattattta catcatttag aatcatctca gcatatggct
     4861 gtaactccac tggtaattgc gcaaatgtgc gatgtaagtc gtttaacaat gattgaatta
     4921 aattcgaatc acgttcttct tttatattca tgtatttttt gaagtttgtg tttaaatact
     4981 cataattaat acgactacga ctaccttcag gtaattggta atcaatgtca tacgctatac
     5041 tatcatttcc gtctctaggt tctctctcta tctcgctgta acgttgtgcc aagatattaa
     5101 attcatattc tgtgattact actgttatag gttcataatc ctggatttca taagtcgatt
     5161 ttgcccatgt gaatccctgt atcatcgctg attcataaca atggattagt tgattataaa
     5221 gatttacaaa ttgcttaatt tcccctggaa cctccggtaa atgactgaag ttttctatgc
     5281 cagaaatatt aaatacggac ttaatttctt cgaatatatc gttcaacttt tctaagttat
     5341 tatataattt ttcaacaaat aacccttttg cttgtttgcc tgagtataac tcaatagcct
     5401 cttcaatgtt ctgttccatc gtatgtgctt tacgataata acgaatggtt ccgaacggtt
     5461 tttcaggacc attaagacga tttgtgcgtg aaaaagcctg aattatgttc tgatattcaa
     5521 gtgacttatc taagtatagg gtgttgaccc attttgaatc gtaacccgtt aagagctgat
     5581 taacaactat aacaagatct aattgggttg cttcatctac tgttttgtaa ggattcttat
     5641 gcgctgtgtt ttgcaatagt tttgtacaca agattgcaaa gaaaagtaca caatgttgcc
     5701 acttctggtt ccatgagttt tatttgtgtt gatgggaaaa ccaatcagct atttacgacc
     5761 cttgaagatc gcagacttta caaactaact caatacttcc tgcgctttcc tagaaaggcc
     5821 cgtttaaagg tcaagtacct agtgatggac atgaatgcca gctattgtca gttgcttaaa
     5881 acggtctttc caaatgcgga gatcgtaact gaccgctttc atattgtcca acacatcaat
     5941 cgcagcttca atcaattacg tgtccagatc atgaatcgtt ttcgaacctc acactctgaa
     6001 aatcagaaga agtaccgtcg gctcaagcga tattggaaac tcttactcaa agattccacg
     6061 acatgggagg cactcaaacg gcattatcat cgactgttta aacgaccaat tagtcaaaca
     6121 gaaatcgtta atgagctgtt aagctacaac gaagaattgc gaactgcgta tcatttttgt
     6181 caattattac gctattattt cgttaaacga gatacggtag gctttcaaga aacccttaaa
     6241 acaatttcat ctacgttacc tcagtcgttt aaaaagaaat tcacgatctt ccatcgctac
     6301 caatcgggta tttcaaatgc ctttaaggtg tcccactcaa acggcgtcac tgaggggctg
     6361 tataacaaga tcaagctgat taaacgaatt gcgtttggtt atcgcaactt ttataacttt
     6421 cgggcacgga tctatttaca acaaggtctg atctttgaaa actaaaaaaa tatgcggcgc
     6481 acagaacgcg ccgcatatcg gactaagttt tattggctct ataatttcta ggactgatgg
     6541 aaattttcta tctgtcctag tttttttgta aaaaaacaaa aagacataca aaatttccgg
     6601 tagaatagag tctcctacaa catcattctg ggggaattct tgtatgtcca tgaatcattt
     6661 tatcaaacaa ctctttacta ttaaagactc aaatatccaa attcaatcct ttgaagaaac
     6721 tttttataaa agcagaaaaa gtatcgttat tgagggaact ctgatccgta cctacagacg
     6781 ttgtccgtgt tgtaaagatt ctacaaagca aatcgttaaa aatggcaaga agatctctat
     6841 gattctcctg aatcgtagcg gtaataaacg gacctatctt cggttgaaga aacagcgata
     6901 cctctgccga gcatgtaaaa aatattttac cgctcggacg tatctcgtta ccccattctg
     6961 ctttatttct aagcagattc attacaagat cttagaagaa ttgacagagc gtcaatctat
     7021 aaaagcaatt ggtaaacatt gcgatgtttc tgtgacaact gttcagcgga cattgtccgc
     7081 cttgtcatca gatatccgtc agaatcttga gtatttacct cggtgtctat tattcgatga
     7141 gtttcgttct ctttctacat ggcacggtaa attttctttt tcttgtatgg acggtgagac
     7201 cgggaaactg tttgagatct tacctagccg ccgtaaaaaa gatctcgttg cttttttcat
     7261 gcgtttttct ctcaaagcga ggttgggtgt acattacatc gtaacggata tgaacgctcc
     7321 ttatttctca ctagttaaag agtgttttcc aaatgcacaa gtgatcgtcg atcggtttca
     7381 cattgttcaa catctgaacc gcttcttcga tgcggttcga aaacgtgtga tgaaaacact
     7441 tgatcaaaaa gacccaatac aagctaaaca gtatcgccaa ttgaaatcct tattcaaatt
     7501 acttttaaaa tcagaggatc gtttagatta caatacacta acgaaaagac gcaatttcaa
     7561 ttggagacta ttgactgaaa cagaagtagt tgaccgcctt ctaaagattt ctcctgaact
     7621 taaaacagct tatcgttact accaagacct gcttactgcg tatcaggaaa aagatccaga
     7681 tacttttttc cagctgcttc gagcaatgcc tggagaagtt ccattggaac tgcaccatat
     7741 caaaaaagcc tttttcaaat acgagaaagg gattcggctg gcattactga agaagtattc
     7801 gaatgcacga ctggaaaatc tcca
//
LOCUS       DACDYJ010000096         7809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDYJ010000096 DACDYJ010000000
VERSION     DACDYJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.097"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="IWM51_002494"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="IWM51_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP9971862.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="IWM51_002495"
     CDS             complement(2203..4389)
                     /locus_tag="IWM51_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP9971863.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IWM51_002496"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IWM51_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP9971864.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="IWM51_002497"
     CDS             complement(4611..5051)
                     /locus_tag="IWM51_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP9971865.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="IWM51_002498"
     CDS             complement(5125..5664)
                     /locus_tag="IWM51_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAP9971866.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6279..7745)
                     /locus_tag="IWM51_002499"
     CDS             complement(6279..7745)
                     /locus_tag="IWM51_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9971867.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2120 a         1723 c         1367 g         2599 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgaggggc tatttttgtt tggctgttct tattacttga tgcaggacag
     6121 ctttttcaca acctctgatt cacggtgttc cagaagtagc ttctgcgaaa aacaagccga
     6181 aacttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac
     6241 cacttctgtc acaacctctt gtaacaagta tttctatttt aaggctgaac cactgtttct
     6301 ggttcagtga tttctttgtt tttgtctcga cgagcaattg ctggcaagat catacctaat
     6361 aagatcaaga caattggtgt gatgatattc gtgaataatt ggaaccacca tgctttagga
     6421 tcagcagtat aatctacttt tggcaccatt cctaggatac atgcaaacgc agtgaaggca
     6481 aagcaccaag caccaaagat aaaaccgatt tttggatttt taacaaattt atattcagaa
     6541 ttgaatttct tataagcttt attcaacatc atatatgcca agaacaccca taggtaacgc
     6601 attggcatga caactgagtt caagttcgtc aaccatttta ctaattcatt catatctttg
     6661 ataccaaaga tcggcaacaa aataatgatt cctactagta tacctgtcag aagataacca
     6721 ttcaccaatg tacctttacg attacgttta cgtagccagt ttggtacaaa ttctggatcc
     6781 gcatctgcta acaagatttt tagtggtgca tcaattgaaa aggctaatgc ggagatttgt
     6841 ccaatcatgt ttgtcaatgc gtaaatgatc atcaatgagt taccgatacc atagtaatta
     6901 cctaaaattt gaaaagcact gtatgcccca tttgccatca agtctttagg aatattatcg
     6961 ctagcaaaca gcatacccat tgcgactgag cctaatacag cacaaatccc taccattgct
     7021 gctaagaaga tcatcccttt aggaaattcc tttgcaggat ttcttgtaga gttgacataa
     7081 ggggatattt tttccgctcc tccaactgca aatactagca tcgaaacggt tgtgaaataa
     7141 ctgaaatcaa atgttggaat atatgttttc acttgattca tatgtggtgt ggcaaattcc
     7201 atcgttggat tgatgaacgg tgccgttaca gctaaaagaa taaataagat cgacatgacg
     7261 aacatcgctg ttccggccag tccgccaatc actttcaaag tagaaagtcc ttttgttgat
     7321 aaatataaaa aggcaagaaa tataataagt gaaataactg ctaccatttg tactgacatc
     7381 gtatttacga tatttccatt tccttggact gcccaaccgc cagcaatcag gattgcttga
     7441 ggcttttgtg ccaaataagg aatatggacg acccagtaag tccaagcggc atagtatgcc
     7501 agtcgtttat tagatgtcgt attttcgacc catgaactga cgcctccttt actatccttg
     7561 aaggctgacc ctaattgtcc aacgatcaat gcgtatggaa taaaataaat ggccaaaatc
     7621 aatagccaag aggtgacaac tgatactcct tgctgagcat agttattaac tacattcccc
     7681 atgccccaaa ccatgttaaa tgcaattaac gcaacagtaa accagcgtaa ttgtttgttg
     7741 ttcattcgtt ctctccttta tgataaaaat ttgttatcca gatgtaatca tattcaattt
     7801 ttaacaaac
//
LOCUS       DACDYJ010000097         7634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDYJ010000097 DACDYJ010000000
VERSION     DACDYJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.098"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(18..368)
                     /locus_tag="IWM51_002500"
     CDS             complement(18..368)
                     /locus_tag="IWM51_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971868.1"
                     /translation="MINYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EISEVLNAAVIKNRLVCIQKEERNAEGYYPDDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(361..1686)
                     /locus_tag="IWM51_002501"
     CDS             complement(361..1686)
                     /locus_tag="IWM51_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9971869.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTERTRSQRRKKLAQMIQEKIKEELGLIA
                     TVGIGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYSIKSRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRNVGITYSKLVYTDSLQLDLFSDPQKQIDEAN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            2135..2749
                     /locus_tag="IWM51_002502"
     CDS             2135..2749
                     /locus_tag="IWM51_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9971870.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2914..3918)
                     /locus_tag="IWM51_002503"
     CDS             complement(2914..3918)
                     /locus_tag="IWM51_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971871.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLE
                     LKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITA
                     CEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEK
                     RNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNK
                     SQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD
                     "
     gene            complement(4377..4634)
                     /locus_tag="IWM51_002504"
     CDS             complement(4377..4634)
                     /locus_tag="IWM51_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9971872.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4627..4896)
                     /locus_tag="IWM51_002505"
     CDS             complement(4627..4896)
                     /locus_tag="IWM51_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9971873.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(5027..5206)
                     /locus_tag="IWM51_002506"
     CDS             complement(5027..5206)
                     /locus_tag="IWM51_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971874.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(5587..6294)
                     /locus_tag="IWM51_002507"
     CDS             complement(5587..6294)
                     /locus_tag="IWM51_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971875.1"
                     /translation="MSNVVYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            complement(6287..6724)
                     /locus_tag="IWM51_002508"
     CDS             complement(6287..6724)
                     /locus_tag="IWM51_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971876.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLDELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            complement(6739..6990)
                     /locus_tag="IWM51_002509"
     CDS             complement(6739..6990)
                     /locus_tag="IWM51_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971877.1"
                     /translation="MKTQHKLLTVEEVVQILVDKEVISEKVAEALLSPRHWTIEIPLV
                     EDGPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            complement(6990..7196)
                     /locus_tag="IWM51_002510"
     CDS             complement(6990..7196)
                     /locus_tag="IWM51_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9971878.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTIECDCGERAKCIKSYRLYQC
                     QTCGKKYTLSYGDYILIDEKGKKR"
BASE COUNT         2245 a         1480 c         1147 g         2762 t
ORIGIN      
        1 atattgctgg ataatgttca ttctaatcga ctccatttat tatcctggta aaattcgata
       61 tgacgtattt cgtcgtagtg aattttgtct ttgccaacgt agatccccag ctcatcacta
      121 cctaatattt tgccaattat atcgtctgga tagtatcctt cagcatttcg ttcttctttt
      181 tgaatacata ctaatctatt ttttatgact gcagcgttca atacttccga aatctcttca
      241 gtcgtcattt gttcttttgc taaattttga ctggttcgtt gttcattgtc ggcatcaatt
      301 tcttcagtat gatcagataa ataaaaccct gcccacttta aacgtccacg atcattgtaa
      361 ttaatcattt ttaattcccc catttccgcc cgcatgacca ccaacaagtg tgcttctagt
      421 aatgcttcta gcgccttcta acattgaaga agcatgtaca atcgacgtaa aaccatactt
      481 ttgtctgatt ttatcaataa ttttatccaa gttcgcttca tcaatctgct tttgtggatc
      541 actaaataaa tctagctgaa gcgaatcggt gtacacaagc ttgctatagg ttattccaac
      601 gttccttact tcttgtcctt cataatactt atcaaacaag aaaagacaat aattctgcaa
      661 ttcctttgta ttatctgtta tagggatctt catctggtgt gaaaaccctg gtctcgtttc
      721 taagatcgac gtgccaatgt gtaaatggac acaacctgtt ttacagttat gctgcctaat
      781 gcgtatcgct acttgttccg ccatttcttt taccaccaac tcgatttcat ttcttctagt
      841 ataatctcgt ggtaacactt gtgaatttcc atatgatttt tcctttgtcg gttctggtgg
      901 gattgctatg tcagttcgat caataccatt tgcatgaaaa taaagctgca agccaattac
      961 acctaaacga cttttgatcg agtaaggatt ccagttagca agatctctaa tactcataat
     1021 ccccatttgg ttgagccttt ttttcattcg cgaaccaatg ccccaaaagt ccgtcatttc
     1081 gggaatattc catactttct caggaacgtt ttcgtacgtc cattccgcaa taaatccttc
     1141 attatgcttt gcttcattgt ctaaggctaa tttagccaaa aggggattat cgccgatgcc
     1201 taccgtagca atcaaaccta gctcttcttt aatcttctcc tggatcattt gagcaagttt
     1261 ttttctccgt tgacttcgtg ttctttcggt cgtaaaaagg ttcagtgatc tggtcacttt
     1321 taagattgat tcatcgattg aatagatcaa tagatcttcg tctgctacat accttctgaa
     1381 aatattattt acctgcatat ttcgcttgat atataggttc atacgtggtg gaacaacgtg
     1441 tagtgtttta ggaaatggtt gtggtaagtc acgtggtcta ctcacatttg taataccata
     1501 ccgctttttt gcttcaggag aagaagctaa tatcaatcct gaaccagtat tatcagctcg
     1561 actcataaca acaagttctg ttgttaatgg atctaaattt ctttctatac attcgacact
     1621 tgcgtaaaag cttttcatat cgattaaaaa gtaatcattt actggctctt ttgaataatc
     1681 catcataaat aacactccat tctttcacat tatacaaaca tatgttcgat attgtaaaga
     1741 agaaatagct aaatacataa aaaagaagca taggtatgct atcaccctgc ttcttctttt
     1801 gatttgtatg atcaatttat tctatcactt tactaattaa tttgaaaatc taataaaaac
     1861 tctcactcta aaagagtgag agaccgcaaa ttagagtacg gacttttttg gagttccgtt
     1921 ctttaatatt aacaaatgaa taaaatcctt acaacatttg agaataagtt atcagtagac
     1981 acttacaaaa aattctcata ctatttcctc catcattagt ttttgtctat ataaggtata
     2041 ccctaaatag acactggtat aacattgaaa aatatggctt cctcgtgtat gattagggtg
     2101 tattttaaat agttgcagaa agtaggtaaa agttttgaga gttgcataca tacgtgtctc
     2161 atcgatcgat caaaatgaac aacgccagat tgaagaaatg aaaaaattcg gtgcagagag
     2221 aattttcatc gagaaacaat ctggcgcaac gattactcat cgacctgttt tccaagaagc
     2281 attagattac gtccgagatc aagatatttt tattgtggaa gctattgatc gacttggaag
     2341 aaattatgac gagatcatcg cttcagtcaa ttacttgaag aagaaaaatg tgaagctgat
     2401 cattactagt ttaccgatca tggctgaagc aattggcaat cctctcctgg ataaattcat
     2461 taaggatctg atcatccaaa ttttggccat gatcgctgaa caagaacgga ctgaatcgaa
     2521 acgtcgccag gcacaaggaa ttaagatcgc aaaagcaaac ggcgtctaca aagggcggcc
     2581 caaattatac agtgcagacg caaaagatcc ccaacgtcgc cttgtttaca aaagcatcgt
     2641 tgaagatctt aaaaatggtg ttgcgattgc taagattgct aaagactaca atgttacgag
     2701 acaaaccgtg tatagaataa aaaaagacag catggtcaat gacaaatagt tgatcatgct
     2761 gcttttatta tcttctcatc tttaatttcc tacatataat tactatctat aaaattaagt
     2821 cttactcaga acagataaag cataaaattc caacaaacca ttttttactt aactaaataa
     2881 agagtctgtt aatataatct tcacttattt acgttaatcc cctactgtat ttgttggttc
     2941 cttatctata ttttctacaa aatccttatt atcttgtgat ttagcttcgt ataattcttt
     3001 gtatgtcgac acattggtta tactaattcc tatagctagt tcttgagact ctacttttct
     3061 ctctagctcc tttgacaaag tgatttgaga tttatttggt tccgttggag tcaattctat
     3121 agattcttca taaaaatctc tatacataaa gctatcatta attttataaa aaaaattaaa
     3181 tgtttttagc actgccatat aacattcaaa acattcttct atagttggtt tacttccttg
     3241 aggagaatga accaatttat tacgtttttc tatgaaattt ttaatttcct ttcttatttc
     3301 tttcggaaac tcaatattaa agtacccttt aaacatgctc cctaataatt tgtctacagg
     3361 gggagattgt aaattctcaa atattagagt taaatcaggt ttctcttgtt gataaaattc
     3421 ctttacccct atttcacagg ctgtaattat cgaaataaac ctatctctag tatttgatat
     3481 atttttactt cttttaagga gtataagaga aaaggggata ctttcgtgtc catgcatgta
     3541 attaactaat tgttgagctt cagaagttat atctctaatt atatagttaa cggaattagg
     3601 aagtgtcttt aactctaatg aaatttctat ccaattacta tcttctaatc tgtatatgct
     3661 cgaagtataa tttgattcgt cagcaagcat cgctacatcc caaacactga tcataaaatc
     3721 ttttaaacgt tcaatcattt cagcaatatt gctaaaatca gctaattttt ctttagaata
     3781 aaaaaaaaca tttatatcag aatcaataaa ttctattttt tttattacta tatcgtcatc
     3841 attatcttga ccatttatgt ttattgttcc ctgagtgggg agtacttctt tcaaattcaa
     3901 gttaaattta tattccatta cttctcctcc aatatataaa aattacttaa ctgtatccaa
     3961 atttaatatg taaataaatc attaattttt agctaaattc tatgagaagt ttatttttgt
     4021 tgtttttgct aattaactaa aactagtaca aaaaatttaa aatagattat taaacctatt
     4081 gcttattcta ttcagtcata tatataagcg tcatccttta gtaactaaca tcccacacta
     4141 caatagtatt ataacatatc tattcaactt caaattgttg aaatttaagc catttatatt
     4201 tacttttcct caactttaga aatgatttta ctaaccttat atccgtaata cgcgtaacaa
     4261 acacattcat taataacaat caacactaag ctatattagc tttttaaatg actagggtaa
     4321 ataaaataag gatcaaaaac ttttaagttt ctgacccttt ccttacttcc gattggttaa
     4381 tagtgatctt ttgcagaata aataaatatc gtttcatttt caactctata tatcagtcta
     4441 tgttcatctg taattcttct ggaccatttt ccagataaat catgctttaa tggctcaggt
     4501 tttcctaatc cagcaaaggg ggaacgatcg atatctttta ttaacttgtt aatctttttt
     4561 atattgcttt tgtttccttg ctcatgccaa taaagataat catcccaagc atcatcagac
     4621 caagccttaa tcatcagatt caacctcgat taagtcatgt gttttaaatg cacctttgga
     4681 gaattgttca tctcctcgac gaattttttc catgacgtaa ttattagaaa gtgttctcaa
     4741 cgtttcttgc atagaatcat aatctctttt tgataataca acaactgtat cttctacatc
     4801 tttacttgtt acaataagtg tttcagcatc ctcattaact tgtttcatat aactacgtaa
     4861 attttggcgg aaatttgaat aagctactgc ttccattcct ttcacccctt atttatttct
     4921 ataaaacaat tgtacattat attgtacaat taagcaaatg attaatattc taccttctta
     4981 taattaaaag tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg
     5041 ctggtacctc aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa
     5101 tcctggattc acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt
     5161 catccaacgt aacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct
     5221 ttcatcgaaa taaccctatg aaaaatatgg ctgcaatcat cttgaatagt agactcacaa
     5281 gggctaatac aaagcgccaa aagatcaaac aaaataccag tattgccaaa aatactacga
     5341 agtttaaatg cacagctgca gcatggcgca taagtatttc gccaacaaat aggactaaca
     5401 atagaggaat gattttattc atatggatct ccttctttct cttaggttat cgggtgttgc
     5461 ccgcttattt cgcaccacct aagcggcggg ggcggttgga gcgattggtg cgaaataaga
     5521 accattccct tagttactag gttttttgtc cttatttcac acattctcac ttttcggtat
     5581 aaatgattat tgcctttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag
     5641 ttcattttgg atttcttctg tatcgtcgta agtataggtg tctaaaaaat cagttaaagt
     5701 cgtgtaccag tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc
     5761 gggtacagcg agttcataat ccattcccat taacacgata gattccgttc cacacgtcaa
     5821 aaatctagta aatacaaggt aataatcgtc tatcgtcgca tctttgctgt ttagctcttc
     5881 catttcttct tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta
     5941 atagtgtttc ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag
     6001 gtaaacattc atgatgtatt tttctgtttc tagtcgtaga agctccgttc ctttttttac
     6061 cgtttcagga cgacgtttat acacgtctaa acgattatgg ctgactgtga ccttcgtttg
     6121 actcgtttca ctttccaaat acaattgttc ttcgactctt tgaataaagt acttcatttc
     6181 ttccatacaa ctaaagtctt tttgaatccg tctgattact tcattcaaat gttctttgtt
     6241 tgttaaaaag tgtgacacat cttccgttgt tagttgataa accacattac tcattttcgt
     6301 tgtcctcctt tttcatggtg taatatgcgc catacttatt gatattttca aaataatcta
     6361 accattcact tgaaacagtc ttagcttttt tcgtttcgac aaattttcca tacgttagtt
     6421 ctaaagcttg gtattcagaa ggtttaattg tgcttggttt gacgcctact tccaagcata
     6481 gtaaggcttg gaaaaaccat tttgcatttt gctttttcag ttcatccagt gttcgatagt
     6541 acgtcgcaat gagattcatt tcgacacctg attcaatcat ttcttccaca gctaccgaca
     6601 atttttcctt ataatgtttt tttggaacac gtgtctcatt gtataggaac gtcatatcct
     6661 ccattttttg tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattctgag
     6721 ccatattttt tctccttttt agttctctaa tttggttaac caatattcgt tttcataagt
     6781 ttccatgttt tcttgataat gcagttcata tcctgctatc ttcactaggt cttcaaaaag
     6841 catggttggt ccatcttcta ctaaagggat ttctatcgtc cagtgcctcg gactaagtaa
     6901 agcttccgct actttttcag aaatgacttc tttgtccact aaaatttgaa cgacttcttc
     6961 cacagtcaat aatttatgtt gtgttttcat cagcgtttct ttcctttctc atcaatcaaa
     7021 atataatcgc cataagacaa ggtgtatttt tttccgcatg tttgacattg gtacaaacga
     7081 tagcttttga tacatttcgc tctttctcca cagtcgcatt cgatagtatg tggcgcatgg
     7141 tagtttggga ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt
     7201 gattctgcct cctcttagga aaggttttac ttcctttcgt tagttctttc tttattgatt
     7261 tgttggccag aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta
     7321 cccttgtcct tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa
     7381 taaagagata acgaacagga acgaaccttt tggtgagagg aagttttgtc tattttgtct
     7441 tgctttgcaa gacaccatat cttagtgaac gtgcagcgtt cacttccttt cttctcgaac
     7501 ggcggtggcg gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct
     7561 aaaaaggtct tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact
     7621 acaactcttc tttt
//
LOCUS       DACDYJ010000098         7623 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDYJ010000098 DACDYJ010000000
VERSION     DACDYJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.099"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(14..706)
                     /locus_tag="IWM51_002511"
     CDS             complement(14..706)
                     /locus_tag="IWM51_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9971879.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IWM51_002512"
                     /pseudo
     CDS             791..967
                     /locus_tag="IWM51_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IWM51_002513"
     CDS             complement(1143..1712)
                     /locus_tag="IWM51_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9971880.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IWM51_002514"
     CDS             complement(1786..2109)
                     /locus_tag="IWM51_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971881.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IWM51_002515"
     CDS             complement(2263..3075)
                     /locus_tag="IWM51_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971882.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IWM51_002516"
     CDS             3429..4277
                     /locus_tag="IWM51_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP9971883.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPLR"
     gene            4422..5363
                     /locus_tag="IWM51_002517"
     CDS             4422..5363
                     /locus_tag="IWM51_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP9971884.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IWM51_002518"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IWM51_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1511 c         1370 g         2470 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattccttt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aac
//
LOCUS       DACDYJ010000099         7600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDYJ010000099 DACDYJ010000000
VERSION     DACDYJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.100"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(138..428)
                     /locus_tag="IWM51_002519"
     CDS             complement(138..428)
                     /locus_tag="IWM51_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971885.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IWM51_002520"
     CDS             complement(546..806)
                     /locus_tag="IWM51_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971886.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IWM51_002521"
     CDS             complement(943..1356)
                     /locus_tag="IWM51_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP9971887.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="IWM51_002522"
     CDS             complement(1812..2297)
                     /locus_tag="IWM51_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971888.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="IWM51_002523"
     CDS             complement(2436..2651)
                     /locus_tag="IWM51_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP9971889.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="IWM51_002524"
     CDS             2875..3675
                     /locus_tag="IWM51_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9971890.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="IWM51_002525"
     CDS             3694..5562
                     /locus_tag="IWM51_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9971891.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELCDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="IWM51_002526"
     CDS             5555..6046
                     /locus_tag="IWM51_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP9971892.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="IWM51_002527"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="IWM51_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP9971893.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="IWM51_002528"
     CDS             6606..>7600
                     /locus_tag="IWM51_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP9971894.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2706 a         1213 c         1444 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatgcga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DACDYJ010000100         7436 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDYJ010000100 DACDYJ010000000
VERSION     DACDYJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.101"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(274..1239)
                     /locus_tag="IWM51_002529"
     CDS             complement(274..1239)
                     /locus_tag="IWM51_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP9971895.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1473..2318
                     /locus_tag="IWM51_002530"
     CDS             1473..2318
                     /locus_tag="IWM51_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9971896.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YPLPQLMKHLIKRFPKVHFEIEIANSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2509..3696
                     /locus_tag="IWM51_002531"
     CDS             2509..3696
                     /locus_tag="IWM51_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9971897.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYFYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVVGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3702..4058
                     /locus_tag="IWM51_002532"
     CDS             3702..4058
                     /locus_tag="IWM51_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP9971898.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            4060..4398
                     /locus_tag="IWM51_002533"
     CDS             4060..4398
                     /locus_tag="IWM51_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP9971899.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            4793..5827
                     /locus_tag="IWM51_002534"
     CDS             4793..5827
                     /locus_tag="IWM51_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9971900.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5820..6506
                     /locus_tag="IWM51_002535"
     CDS             5820..6506
                     /locus_tag="IWM51_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9971901.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6530..7378
                     /locus_tag="IWM51_002536"
     CDS             6530..7378
                     /locus_tag="IWM51_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9971902.1"
                     /translation="MKKKIFGLAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDNIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
BASE COUNT         2449 a         1328 c         1619 g         2040 t
ORIGIN      
        1 ctttttcacg acctcttggt aaacggtgtt ccagaagtag ctttttcgga aataagccga
       61 aatttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctggc
      121 cacttctttt acgacctctt ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagc
      181 ccaaccaatt caaaaacatg aacagctgtt tttgtttggc cgttcttatt acttgatgca
      241 gaacagcttt ttcacaacct attgaatcga ttattaagcg atttttttag aaacaaattt
      301 tccatgtttg ctatgttctt taattaagaa acgtacgtaa tgagcaagaa cgtagaagac
      361 aactacactt gcaaacagac attccgcaac ttttaagaaa tccaatacgc tactatagat
      421 acctaaactg ttgaagtaat gaacagtagt aaatatagct gttgcagaga taacaagcgg
      481 gaatgtaaag gctgcatagc ttggataaaa acgaagcttg aacaaacgtg gaagcataga
      541 cagcacaacg aaatacaata cttgagataa agcgaacaat gctacgacaa gataaaaatt
      601 aggttcagca aagtttttaa gataacctgt taggcaaaga gaaccaggtg ctgcaacgat
      661 cgtaatcagc ggacgtgtcg gatgagccat atttcttacg aaaaagacac gtttgatgat
      721 gatcggcaat aagaacgcat agcagatcaa tgaaatccaa aagaaaattt ttccaataag
      781 cgggaagaag ttcccggcag ttacagtgat gaccccaaat ccaacataaa tgataaacca
      841 gcttggataa attgcttcaa ttgaaagatc agctttgata acatggtgat aattgaaata
      901 aaagacaagg atcacttgga agataacagc gcacagccaa atgaatttga caattggtgc
      961 aatactttca tatgaaacaa aataagtaca aataaccatg agtgacatcg taaaagttgg
     1021 tgaaacggag gcgatgattg gatcatgcaa cgtttgtttc gtgtgttcga ataagataaa
     1081 caatttaaca aaaatgaata tcataagaat catagagata ccgccagtta gattgcctaa
     1141 agcaacatgg tgatagtctt taaacagatt tccaagggaa gctaacccaa ggatcaaccc
     1201 gcagatcggg atcggaataa ttttcaaaaa gttcttcata tataatattc tcctttttat
     1261 ttaatagatt ttagtaccaa gatgaacgaa tacattgtaa aatgaatagg aatgtatgat
     1321 tacgagcaag cagacagctg attgtcttca tctaagttac tagttggatc agcaactgtt
     1381 ttgttgtaca tttctattat agatggtttt ttgtatattt aaatcaaata gtttttatag
     1441 taattatcat aaaaagttat ggaagtggtg ccatgtttaa gttattaacg acttttcgag
     1501 cagtatatga aacaagaaat ttttccagag cagcagaagt actgttttta tctcaaccag
     1561 cagtctcgaa tcaaatcaaa cagttggagc gggaattgaa tatcgatctt tttcaacgta
     1621 acggaagaaa agagatgctt ccaactaagc aagccgactt attatatgaa cggacactgg
     1681 ttttgttaga agaatgggaa gaaactcagc aaaaattgct caatgaacaa aatgaagaag
     1741 aagtatgtcg aatcgttgct tcccacacct ttgctgtcta tcctttgcct cagttgatga
     1801 aacatctgat caaaaggttt cctaaagtgc attttgagat cgaaatcgcg aattctcatg
     1861 aatcattaga acaagtagcg aaacatgaat ccgattttgg ttttatcgaa aagccattag
     1921 agacttccgg cgtacaaaga tactctttga tggaagacca gctagtcatc gctggggacc
     1981 caaaaagcaa attgtggctt gtaagagaag caaactctgg tgtttttcat tacacgcagc
     2041 gttattttga agagatgaat atccaagata agaagatgat cgtgaaaagt aacgatgtaa
     2101 ttgttgcgtt gctgaaggaa ggaatcggtc gctcgatttt gtctaagcgc tctgttccag
     2161 aaaatgtgcc aatgactccg ttgaatttcc atcgttattt ttatttcatc caacgaacca
     2221 atctggtttc tgatcagctg ctagagattg ccgaagagat ccttcgatac aatgaaagca
     2281 cagaaacttc tcacagcgag gagaatctca gttggtagat aattgcatgt atcaaaaaaa
     2341 gttctatgat acactaaaga taagcagtag gcttttgtac tgcatttgct caagcaactg
     2401 cacaaacgga aagttaattt ttcccaaatg tctatggaaa gactttccat tatctaactg
     2461 gaccgctata ataatcagca gtatggtttt taagaaaagg gatgaaacat ggaaaaaaaa
     2521 ttaaaagctt cgaacgataa tcgtattgat tatggcgtga ttttaccagt ctttctgtta
     2581 tgtttgattg gtctgctctc attatatgta gcacttagcc atgatccgaa caatccagca
     2641 gtcttgaaag gcgtagggat gcaagttgtc tggtatttag ttggagcggc agcaatcgtt
     2701 gtcatcatgc atttgaattc caaatggatc tggaaactga ctccttactt ttatggtttg
     2761 gggttagtgg taatgggact cttgctcaaa ttctatgatc agaatttagc tgcttcaaca
     2821 gggtctaaaa actggtttcg attcggtgcg tttactttcc agccagcaga gttgatgaag
     2881 attgcgtaca tcttgatgat ggcactgatc gtaactaaac ataatacgca agtaaaagaa
     2941 cggacgatga aatcggactt ttggctgatt gggaagctct tgatcgtgac aataccggtt
     3001 ttagcattga tcatggccca agatgacttt ggaacgatgc ttgtctttct tgcgatcttt
     3061 ggcgggattt tcttgatgtc tggaatctct tggcgcatca tcgcacctgt agtaatcttg
     3121 gcagtagtcg ttggagcagg aacgatcttc ttggttacga ctgaaggcgg aagagacttg
     3181 ctttacaaag ttggcttcaa atcttatcag tttgcacgta ttgattcatg gctggaccct
     3241 ttccatgata cgtcaggaat gagttatcag ccggcccaag gacttttggc aattggtacc
     3301 ggtggcttgt ttggtaaagg attcaacgta agcaatattt atgtgcctgt tcgtgagtcc
     3361 gatatgattt ttaccgttgt cggtgaaaac ttcggcttta tcggtggtgc atttgtcatt
     3421 ttcttgtatt ttattttaat ctatcgtatg attcgggtgt gtttcgatac gaataatgaa
     3481 ttttacgcct atatcgcaag cggtttgatc atgatgctgc tgttccatgt ctttgagaat
     3541 attggagcaa atatcggatt acttccttta acagggatcc cattgccttt cattagtcag
     3601 ggaggatctt cgattttagg gaacatgatc ggtatcgggt tgatattatc gatgagatac
     3661 caaaatgaag cacctgcaag atcttcaagg aggtaagaaa attgtacacc ttttactggt
     3721 acccgaaatg ttcaacatgc aaaaaagcca aagcgtggtt agaccagcat ggcgttagtt
     3781 atcaaactgt tgatatgatc aaagaagtac cgaaggcctc aacgattgag acttggctag
     3841 aaacgagtca agtaccaatc cgtcgcctgt tcaacacaag cgggatgaaa tatcgggaac
     3901 ttggactgaa agatcaagta ggcgagttta cggcttcaaa agcgagccaa gtcttatcga
     3961 cagatggtat gctgatcaag cggccactga tcttacgaga ggaccagctt gttgcaattg
     4021 gattcaatga aaaaatttat gaaggagaac tgatataaaa tggcaggcaa gcatttgaag
     4081 aaaaaggaca acctttggat cttatttaat ggaaaagaat actgtgttgg tttgacaaat
     4141 gaagcgcaag aagaacttgg agacatcacg tttgccaatc tgccaaaagc aggacaatcg
     4201 ctaaaaaaag atgaacctct gatcgaagtt gaagcggaaa aagcggtcaa tgaatttgcc
     4261 agcccgctaa cgggtacagt ttcttctgtt aatgataaaa tcggtgaaga tatcaatatc
     4321 ttgaatgatt cagatgaaat gaatgcgtgg atcttaagtt tcaaagatgt tgatccaaaa
     4381 gaatttgacg aattataaaa tcagaaaatc acttgaaaaa agttgacact gattcttcca
     4441 gttgctataa tcataagcaa tcctatatga aacgagaaag ctttgaaaag aagagtacat
     4501 gtcacggacg tttgaagaga gccctgtttg ctgaaaatgg gtaacgaaaa tgagatggaa
     4561 gatggtctta gagcagagta agcgagcgta agcaaactta ctacgggagt gcccgttaca
     4621 gcgcgacagt atacggaaag aagctaagtc caagtactgg aagaggctgt tattgcgaag
     4681 taacggtaaa tcaaggtggt aacacggaag ttcaagcttt cgtccttttg cttaaggcaa
     4741 gagggcgaag gcttttttgt ttgaaaaatc ataagagaaa gatgtgaatg gcatgtcatt
     4801 aatccaacta acgaatgtca aaaagacatt caatgggaaa aacggaaaca tccaagccgt
     4861 caatgacgtt tcactaaacg ttgaaaaagg ggatatttat ggaatcgtcg gctattctgg
     4921 cgcaggaaaa agtacattag tccgtctcct gaatggactt gagcttccca cgagcggcga
     4981 agtgatcgta aacgatcaag atattacaaa attaaaaaac aaagaattac gtacattccg
     5041 aaagaaaatc ggtatgatct tccagcattt caacctgtta tggtcaagaa ctgtacttga
     5101 aaatatccag ctgccgctgg aattagctgg cgtaccaaag agcaaacgta aagaacgggc
     5161 agaagaactg ttgcgtttag tcgggttaga aggacgcgga caagcatatc cttcacaact
     5221 ttccggcggg cagaaacaac gcgtcggtat cgcacgagca ttggcaaatg atcctgagat
     5281 cctgctttgt gatgaggcta ctagcgcatt ggacccgcaa acaacagaag aagtattaga
     5341 cttattgtta gcgatcaata agaaattgaa tttgaccatc gtcttgatca cacatgaaat
     5401 gaatgtgatc cgcaaaatct gcaacaaagt cgctgtgatg gagttgggga aagtcgtaga
     5461 agaaggagac gtattgactg tcttcagaca accgaaaaaa gaagtaacca aacgctttgt
     5521 acaacaagac atcgaaccag aagctgattc gacagaacta ttagctgaac tcatcaaaga
     5581 aaatccaagc ggccgtttgg caactttgac ctttagcgaa agaaatgcca acgagccagt
     5641 catttcccaa gcgattcgac gctataacat cgatgtcaac gttgtgtatg gaaagatcaa
     5701 acaagtgaaa gaaggttcct ttggttcatt gactgtcttg atgactggtg aaaccaaaga
     5761 attagatcaa gcagaagtgt tcatcaaaga acaaggagta ggaattgagg tgatccatcg
     5821 tggataaaag cttttcagaa acatatctag attttaaaca aatcaacccg gaaacgatga
     5881 atcaggctat ccttgataca ctatatatga cattaatctc aatggtctta gtgacgatca
     5941 tcggattagt tttgggattg atcctttact ctattggacg caaaaacaaa ccgtatgctc
     6001 gaatcattta tggaatcgta tcgatcatca gtaatatttt ccggtcaacg ccatttatga
     6061 tcctgatggt tttgatcatt ccatttacta aagcaatcgt aggcactatg ctaggtgcgc
     6121 aagcagccat tccagctctt gttttatccg cagcaccatt ttatgcccgt ctagtagaga
     6181 ttgcttttcg tgaagtcagc cctggtgtac tggaagcagc agatgcaatg ggagcaagtt
     6241 actgggaaat catctggaag gtcttgatac ctgaaagtat tcccgcattg atttctggat
     6301 taacagttac aacgatctcg atgatcggat ttactgcgat ggccggcgcg atcggcgcag
     6361 gtggacttgg aggtcttgct tggcaggaag gctaccagcg tggtaatctg acagtgacat
     6421 tcgttgcgac tttaattatt ttactaatcg tatttatcgt tcaaggaatc ggtgatttct
     6481 taactaagaa aacagacaaa agataatagg atataggggg atgtaggata tgaaaaagaa
     6541 aatttttgga ttagcagcag cgcttttgct gactgtagga ttagcagcat gcggtaacaa
     6601 cagcgacagc aaagattcag caaataaagc agacgacacg acattgaaag tcggcgcttc
     6661 accgacaccg cacgcagaga ttttggaaca tgtgaaacca ttattgaaag atgaaggaat
     6721 cgatcttgaa atcgtcaaat tcgatgacta tgtattaccg aaccaatctt tggaagaagg
     6781 cgacattgat gccaattact tccagcatat tccttatttg aacaaagaaa tcaaagaaaa
     6841 aggatatgac tttgtcaatg ccggtgctgt ccacatcgaa ccaatgggct tgtactctaa
     6901 aaaagtcaaa gatgtttccg aattaaaaga tggtgcaaca atcattactt caaattctga
     6961 atcagactgg ggacgcatca tcacaattct acaagatgct ggcttagtca aagtcaaaga
     7021 tggagtagat ctggaaacag caacattcga taatattgaa gaaaacccta agaacttgaa
     7081 gttcaaccat acgatcgacc catcactttt agcttctacg taccaaaatg atgaaggtga
     7141 cctagttgca atcaacgcaa acttcgctta cggcgcaggg ttgaatccat tagaagatgc
     7201 cgtattgctt gaaaaggaca actctcctta cgtgaatatt attgcgactc gtaaaggtga
     7261 tgaagacagt gacaaaatca aaaaactgat tgaagtgctt caccgcaaag aagtccaaga
     7321 atggatcaca gacaaatggg gcggctctgt taaaccagta aaggctgatg caaaataagg
     7381 tatggaacaa aaaagatgag aaataagtct gtaatggctt atttctcatc tttttt
//
LOCUS       DACDYJ010000101         7344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDYJ010000101 DACDYJ010000000
VERSION     DACDYJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.102"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            140..460
                     /locus_tag="IWM51_002537"
     CDS             140..460
                     /locus_tag="IWM51_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971903.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="IWM51_002538"
     CDS             912..1112
                     /locus_tag="IWM51_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9971904.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="IWM51_002539"
     CDS             1259..4048
                     /locus_tag="IWM51_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9971905.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="IWM51_002540"
     CDS             4095..4622
                     /locus_tag="IWM51_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9971906.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="IWM51_002541"
     CDS             4643..5833
                     /locus_tag="IWM51_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9971907.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="IWM51_002542"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="IWM51_002542"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP9971908.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
BASE COUNT         2439 a         1340 c         1576 g         1989 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     6721 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtgat
     6901 ctgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtg
//
LOCUS       DACDYJ010000102         7277 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDYJ010000102 DACDYJ010000000
VERSION     DACDYJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.103"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            262..819
                     /locus_tag="IWM51_002543"
     CDS             262..819
                     /locus_tag="IWM51_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HAP9971909.1"
                     /translation="MSIINTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLD
                     KNSFSGGQLFGKNPGGEFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGL
                     GACVIVINYDGLNMVYQEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWR
                     QAQGHAFIDDGTWIDPLPFLNSSKK"
     gene            835..1479
                     /locus_tag="IWM51_002544"
     CDS             835..1479
                     /locus_tag="IWM51_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971910.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            1490..2296
                     /locus_tag="IWM51_002545"
     CDS             1490..2296
                     /locus_tag="IWM51_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971911.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            2319..2548
                     /locus_tag="IWM51_002546"
                     /pseudo
     CDS             2319..2548
                     /locus_tag="IWM51_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2707..3642
                     /locus_tag="IWM51_002547"
     CDS             2707..3642
                     /locus_tag="IWM51_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAP9971912.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            3686..3955
                     /locus_tag="IWM51_002548"
     CDS             3686..3955
                     /locus_tag="IWM51_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971913.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            4232..4510
                     /locus_tag="IWM51_002549"
     CDS             4232..4510
                     /locus_tag="IWM51_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971914.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHALLDYKKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIEQ"
     gene            4549..4971
                     /locus_tag="IWM51_002550"
     CDS             4549..4971
                     /locus_tag="IWM51_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971915.1"
                     /translation="MAKATRKHIYLYDMHIEVLDEIISTHDDISNYSEAVRHLCMMYD
                     PKTEEKISPSQIKLNAIGKEVSILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            4991..6583
                     /locus_tag="IWM51_002551"
     CDS             4991..6583
                     /locus_tag="IWM51_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971916.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTLEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEYGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTEREKIEYQRLNQAMEKYQAEPHKKTSGTLPGVFSISSDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEVGLKAAGRK
                     MMETLFEKEKLNDTGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNSLTKVVKEPVRYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDENRTKLSPYTREKLDELTQYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QTQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            6613..6948
                     /locus_tag="IWM51_002552"
     CDS             6613..6948
                     /locus_tag="IWM51_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971917.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            7089..>7277
                     /locus_tag="IWM51_002553"
     CDS             7089..>7277
                     /locus_tag="IWM51_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971918.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYPF"
BASE COUNT         2767 a         1051 c         1510 g         1949 t
ORIGIN      
        1 tattttggga ttacgtggtt tgatgggtgt ctgtttccta aaggaactgc gcgtggaatt
       61 ggtggcattg aaggtggctg gtacatgaag tttcccaact caaaagctgc cttctcctat
      121 tacggcttta tggtagccac acaatcaaat tttaatgctt gtgtcggaaa taaaagccct
      181 ggcgcaagct tattgatctt aggacgagga gggtatgccg ccgcaggaat tactgaagac
      241 agtgcctatt ataaaaattg tatgtctatt attaatacaa ataaattgat ggagtacgat
      301 gagtttgcga ttaaacactg gagagaaggc ggcggaactg gtggtacgat tacaggaagc
      361 tggggcaatc cattcccagg aagtagctta gataaaaatt cgttcagtgg aggacaactt
      421 tttgggaaaa atccaggagg agaattccga cctaacgggt tccatgatgg actagacttt
      481 ggttcagtcg atcatccagg aagtgagatt catgcggttc atggcggaaa agtcgtgtac
      541 gttggcaatc ctggtatttc aggtttgggt gcgtgtgtga tcgtgattaa ttacgatggc
      601 ttgaatatgg tgtatcaaga atttgcaaat agtacaggaa attctcgagt gaaagtaggc
      661 gatcaagtga aagttggtca agtaattggg atacgagata cagcacactt gcatttagga
      721 tttactcgaa tggattggcg tcaagcacaa ggacatgcat tcattgatga tggtacatgg
      781 atcgatccgt taccattttt gaatagcagc aaaaaataga tcaggagaaa aaatatggaa
      841 aataaaacct taaaaatcgt cttaacagct gcaattagcg ttagtttaat cggcaatatt
      901 gcgttgtttt ggcagcttaa agaagaacaa gagacaaata cctataaaat tgaagaagtg
      961 gctaaaaacg cgtctgataa gctcaaaaaa aattcttctg aagcacaggc gaagattgac
     1021 aagttaaatc aaaccatcaa tgagaaaaac caagaaatcg atcagctgaa gcaacaagat
     1081 ggcacaaaag caaaagagga agtaactgaa gcacaaagaa agacggcaga agagttcggt
     1141 caattggcca ttgataaatc attaagcaga aatgaattaa cggaaaaact aaaagatgtc
     1201 gcaacccaag aagtgatcga caaattaagt cctgtagacg atgatcacca acatgatgat
     1261 tacggaagtt actcttttac attaggtgac gtgcaaactt acgctcaaac aagcagtgta
     1321 aaagaggaac aaaattttgt cgtatttgtt gattatacaa tcggcaatcc tcaattcaaa
     1381 gatgtcaaac cacaaaaaat aaaaggtgga ttaactgtca ctgaaaaaca agtagatggt
     1441 caatggaaag tcacagactt tagttatttc actcgttagg aggaagaaaa tgagtaagaa
     1501 aaaaggagca agacaacgtt ttaaaaaacg aaaaagttat cttagatggc tggggatctt
     1561 tatgattctc ggctttttga ttcttgctag tccgatttac acacataaaa aggtagacat
     1621 accagatgaa gtaagcacga acgcattttt aaacacgaag gaactgacga tggtttcaaa
     1681 agaaaaagga gcgaaccagc aattagtttt tgaatttgcg atcgacgatg aagatcagag
     1741 cttattaaaa gaactagcta atcttaatta tcgtgtagaa gtaaagacag ctaaagggga
     1801 ttatcaagca ataaaagcca aagtgatcaa ggtgtctgat gattattttg tagtgaagtt
     1861 agcacatgta ccagaagaat atattgctat gcgattaact attattcctg aaaaaattga
     1921 tccaaaggtg gatatgcaag aaccgcaaga tctgattttt tatattcacg aagataaggt
     1981 caaagatcac gtgaaagacg aagattatga acaacatgcg atcaattaca aagtgaaagg
     2041 atacaagaaa gaacaaaaag cagcacaaaa acaaattgaa tccttccaag caaccattga
     2101 tttgaatgaa gaattagtga agaaactaaa agatcaattg ccttatcaag tcgcagaaga
     2161 tcaggaaaat actgaaaata agattaatag ttaccagcaa gaaattgaaa catcgaaaac
     2221 gcaaatgaaa gatcaagaag agagcattca aaaacttcaa gaaaaaattg atcgtatgac
     2281 aaaagaaaat atttagaaga ataggtggag taaaagaaat gaagcgagcg aacttggtcg
     2341 gcaaagatac tttcgctgag cagaaagtat tgattaaagc ctatctcaaa gttgtaaaag
     2401 ctaaagaaat atgtcaagaa gtgaattatc taacacaaca atatctgagt tgaagaatgg
     2461 tagaaagagt ttagatacct taaaattcag atcatttgaa gctctatata tagctgctaa
     2521 aaaatatttt acagctgata ataaataaaa aattaaggtg ggttctatgt gaaaaaaacg
     2581 tatcaaaagt ttttatggat tagctatatc gtcctattta tcgttgggtt tctcgtcagt
     2641 gtgtccgtga tcgtcggaac gctttttctt ttgttctctg acgaagatct ggtacttaag
     2701 gggttaatga gtacgttatc gtgtgtcatg ttactttatt tatgtataca gatcaacgaa
     2761 attatgcccc ctcattggca agaaaagaag aaagaattta aggaagaaat cgaaataaaa
     2821 tccaatgact ggattgaata cacatggctt cctctgaaaa gaaatagcaa acgaaaggaa
     2881 ttttgggcat tgcaattatg gccaatagga aaacttttta tttatatggc ttcacttatg
     2941 gttactttaa tcggttattt ggtggtggtc tatggggtgg cctattactt gttaagtgag
     3001 ccaaaacaga tcgcggcgtc aaatattttc acggcagtaa tgacattaat gtgtgtcatg
     3061 tgcttttcta tttttagtta cttactcgtc caatttatca ggcaaaattt aacaatgagt
     3121 aaacttttca aaagtttatt ggacttactt atcttaggca gtatgttaac gactctaaca
     3181 gatcggttcg aacaatctga aaaaacggta gaggggataa agaacttctt aacgacgatt
     3241 gatcaattta taaatggata tatgtatttg tttgaagtag gctttattct cactttgttt
     3301 ggttgtgtat ttataaaact cattaaaaga tcaatcgaaa aagaaatggt tattgatcta
     3361 attccgacgc tgagcggcta tgagtattac gttgattttg gaaagtatga ggagttgatc
     3421 catacggata tcgttcacac gagtatccat gaagtaaccg ttaagggcat cagatctact
     3481 ttaaaagatg gacaggagac gaaaacaatc gaccgaagaa aagaggtcta caatttttat
     3541 aaagttgaag caagtctacg atatggcatt gggaaattga ggttagatgg gagtaatatt
     3601 caacaaaaca aaccatatgt tttaggaaaa gtggtgattt agaacttcct atgaaagatg
     3661 aataaataag gagaatcttt tgaaaatgat tcttctttac agaattgtgc ttatagttgg
     3721 cacggagtta aaaatcttaa agaaggagca gttattcaaa gtgaaagaac atcataatca
     3781 gttactatgt attaaaacgg gtacgatgaa cattcaatac cgaacagaat atttagaatt
     3841 aacgaatcaa actgattttt tgtcgattcg agagcaacat aagcaagaaa aattaatggc
     3901 tacttatagc caattgattg atgagtggga acaattcaag gaatctaaaa cataaaattc
     3961 catttaaaca ccttattttt taaagagcat aaggaaacgt gtacccactt tcaaaataaa
     4021 gtgggtagat aggctgtgcc tatcacgttt ctgaaaaaaa taaatgaccc caaaattttc
     4081 aatttatgtg taggtttatg ggtagcaaaa atatgaatgt gggtagcaca aaagttcatt
     4141 tgtgggtagt aaaatttaaa tatgtgtagc aatgtgtgta gctacacaca ttgctacaca
     4201 cgttataaca tatagtcaaa aggagatatt tatggaagta aaaataagag atattgatga
     4261 aaaaacgatt caaagaattg accgaagatg tattgaatta agtgaacgta ctgggactaa
     4321 atggagccga aatcgatacc taaaagtttt gatcgaaaat gattttgatc acgctcttct
     4381 ggattataaa aaagatcaat ttgatcggtt gttagaaaaa tttgtggata ttcaaactta
     4441 taatacgaaa attttagaag aatatattgc aacaaataat caattaatcg ccttgttaat
     4501 tgaacagtaa aaataatatt gtgaatttat aaaaggagga aatcaacttt ggcgaaagca
     4561 acaagaaaac atatttatct ttatgatatg catatcgaag tacttgatga gataatttct
     4621 actcatgacg atatatccaa ttatagtgaa gcagttagac atttgtgtat gatgtatgat
     4681 cccaaaactg aagaaaagat aagtcccagt caaatcaaac taaatgcaat tggaaaagaa
     4741 gtttctattt taactgaaat ggtttcggag tttgcagatc ttcatttaaa agatacagga
     4801 atattaattg gtacaaattc aactgtttat caagaagcga aagagcgtgt ggaaggcaag
     4861 ataaaagcaa atcaaacgaa gaaatattct cctaagataa agaaaacaat aagcgaatca
     4921 gtgactacaa aaagtgaagt atttgatcca acaaggtttt taaaacgata aaaattagaa
     4981 ggagaacgtg atgagtaaat cacctgcagt cattctcact tgtcaattta ccttgcccaa
     5041 taaaaaatct ttttcaacat acattgatta tatgacaaga gaaaaagcgc tggaagaaat
     5101 ggaacggcgc acgctcgaag aagaacagga actgcaacga atcaagaatg ctttagagga
     5161 tttttatatt cctgaaggag aaactttttc ggagagtaag acggataaag aattcagtga
     5221 aacgtcgaat gaagcgcaat cgttgatgga atatggaatc aattttgaca agttagagga
     5281 acaagatttt accaaatatc tttcttatat gacgcgtcac tatgcgttag aacaaaaaaa
     5341 ggaactaacg gaaagggaaa aaatagaata tcaacgtttg aatcaagcaa tggaaaaata
     5401 tcaagcagaa ccacacaaaa aaacttcagg aactcttcct ggagtttttt ctatatcttc
     5461 agacgaagta aaaggaagtg atctgaagga gattaagcag atcttccaaa gagggcaaca
     5521 aaaaggctca atcatttatc aagatgttgt ttctcatgat aattcgtatc ttgaaaaaat
     5581 aggattgtac gatcccaaaa cagatacttt agatgaagtt ggactaaaag ctgctggaag
     5641 gaaaatgatg gagacgttgt ttgagaaaga aaaactgaat gatacgggat attggatggc
     5701 taccattcac cgtaacacta agcatattca tattcatttt gcagttgttg aaaaagaaaa
     5761 tacgcgtaaa ccatacactg aggtcgaaaa tggtgtgagt tatatcgtac cgaaaggaaa
     5821 gagaagacaa gcaacattag acgcaatgaa gaccaattat gtgcatgaat tagaacgttt
     5881 ttcttatgaa aaggatcgaa tgataggcat ggagaaacga aatttattaa cgcggaaaag
     5941 tgatttgcgt aacagcttaa caaaagtggt aaaagagcca gtacgttatg atcgacacgc
     6001 aatgaactta ttaaatgaag tatataaaac cttacctgac aaacgttcgg aatggaatta
     6061 tggggatgaa aatcgaacta aactttcacc atacacaaga gagaaactcg acgagttaac
     6121 ccaatatgtg ttaacaaaag aacctgaata ccaagaatat atacagattt caaaagcatt
     6181 gaaagaagaa gcaaaaagtc tttatgggga aagcaagaga gaatcaaagg atgcagagaa
     6241 aaatgctctc tttgatttaa aaaagcgcac aggaaatgcc atcctaacag agctgaagag
     6301 acaggatagt caaataaaac cattaataga aagtgttgat cagacgcaat ctatttacaa
     6361 tgaacgatcc tttcgaacgc ttaaacaatt gccaacgaat gaatacaaat atagttacga
     6421 agaattcatg aagcgcagga aacaattcca acaaagagtt gtcacgcagc ggagtttgcg
     6481 tcaattaaat cgaacgatta atgcccatga agaaaaatac cacgcaagta aagcatacga
     6541 agagcaacaa cgaagaatcc aaatggaaca agaaagacag taaagacagt aaagacagta
     6601 ggaggaatag caatgaaaca atatttagat caatggaaag ttatagaagg atcattaaga
     6661 gaagaacgaa tcgaacaact tcccgattgt ttagaaaaag agcatttgtt tcagataaga
     6721 gaaatgttga taaacgaaca gtttgatcca aatcaatttt tagtcgttga gtacccagct
     6781 actggtgtgt actgttgtaa tcatgtgaag ggtgaaaaat attttatcat tcaagaatat
     6841 gagggcaagt tagcgcctta ttatactact tgggaaatga atgaagaggg gatcaataac
     6901 ttcccgtgta aaagcattga agagtctatt tcactcacag aatgttagtt gttaagtgaa
     6961 taaaagaagt aaaagagtcc tgaacgtgat tgtccaggac tctttttttt cactgaattg
     7021 ttttccgcca attcattgag tggtgcgatt tccgtctaca ttatatcaaa agaaaggagg
     7081 aagaagaaat ggcttattca acgattggag ttgttgttga aaaaagcaga gacaatcttg
     7141 tttttgtgac agaaattcaa acaggcagag cttttgtagt cacagacaaa gcggcaaaag
     7201 catatcaaag tggcgatatt ttaacgttaa atctgactac gaagacgttc gtcgatgcgg
     7261 cagaagatta tccattt
//
LOCUS       DACDYJ010000103         7137 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDYJ010000103 DACDYJ010000000
VERSION     DACDYJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.105"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IWM51_002554"
     CDS             591..1280
                     /locus_tag="IWM51_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9971919.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IWM51_002555"
     CDS             1267..2475
                     /locus_tag="IWM51_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9971920.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IWM51_002556"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IWM51_002556"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP9971921.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWM51_002557"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWM51_002557"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP9971922.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            6541..7137
                     /locus_tag="IWM51_002558"
                     /pseudo
     CDS             6541..7137
                     /locus_tag="IWM51_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2210 a         1332 c         1393 g         2202 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta tcccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgcggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtgtaccga cccccaaaag ttagaccaaa aaatctaact tttggaggtc ggtatttttt
     6541 atggctaagt atacttttga acttaaatta aagatcgttc atgattattt ggatggcaaa
     6601 ggcggaagtg attatctggc aaagaagtat tccattaagg ctccatcaca agtgaagcgc
     6661 tggatcaatg cctatcaaga atttggtgaa gagggtctag taagaaagcg acaaaagaaa
     6721 aaatattctg ttcaatttaa gctggatgcg atagagttat atctaacaag tgagctgtcc
     6781 tatcgtgaag ttgccaacac acttaacatg aacaacccta atttgatcgc tagttggatg
     6841 cgtcaattcc gtgagggtgg gatcgatgga ctctcaaaaa cgaaaggatg tccacctatc
     6901 ttgtctaaga aaaatgaacc caaaaataag aaaaaatcta caactaaagc aacgtctaaa
     6961 gaacgtgaac gcatcgagga gttggagaaa cgagtccgtt cccttcaaat tgaaaatgcc
     7021 tttttaaaag aattgaggaa gctgcgaaaa caggaagccc aacaacgacg aatgaagcaa
     7081 tcgcacgaat catcgcaagc ctccgaggac cgtttaaatt agttgagctt ctagaaa
//
LOCUS       DACDYJ010000104         7103 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDYJ010000104 DACDYJ010000000
VERSION     DACDYJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.106"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..816
                     /locus_tag="IWM51_002559"
                     /pseudo
     CDS             1..816
                     /locus_tag="IWM51_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
     gene            1062..1658
                     /locus_tag="IWM51_002560"
     CDS             1062..1658
                     /locus_tag="IWM51_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9971923.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            1671..2570
                     /locus_tag="IWM51_002561"
     CDS             1671..2570
                     /locus_tag="IWM51_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9971924.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEDIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            2573..2704
                     /gene="ssb"
                     /locus_tag="IWM51_002562"
     CDS             2573..2704
                     /gene="ssb"
                     /locus_tag="IWM51_002562"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9971925.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            2726..3058
                     /gene="ssb"
                     /locus_tag="IWM51_002563"
     CDS             2726..3058
                     /gene="ssb"
                     /locus_tag="IWM51_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9971926.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            3103..3336
                     /locus_tag="IWM51_002564"
     CDS             3103..3336
                     /locus_tag="IWM51_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971927.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(3495..3617)
                     /locus_tag="IWM51_002565"
     CDS             complement(3495..3617)
                     /locus_tag="IWM51_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP9971928.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            3807..4031
                     /locus_tag="IWM51_002566"
     CDS             3807..4031
                     /locus_tag="IWM51_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971929.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            4474..4680
                     /locus_tag="IWM51_002567"
     CDS             4474..4680
                     /locus_tag="IWM51_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9971930.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            4680..4931
                     /locus_tag="IWM51_002568"
     CDS             4680..4931
                     /locus_tag="IWM51_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971931.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            5112..5384
                     /locus_tag="IWM51_002569"
     CDS             5112..5384
                     /locus_tag="IWM51_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971932.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            5829..6008
                     /locus_tag="IWM51_002570"
     CDS             5829..6008
                     /locus_tag="IWM51_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971933.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            6067..6423
                     /locus_tag="IWM51_002571"
     CDS             6067..6423
                     /locus_tag="IWM51_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971934.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         2570 a         1161 c         1443 g         1929 t
ORIGIN      
        1 taaaggcaat cgaaaaggtg ccgctagatc taggttggaa gaagttattt ggaaaagaga
       61 gcgacggaaa agagaaggaa gaagagccgc tacttcctaa agtgacaaaa ggagaaatgg
      121 tgacggtgga tctacaagtt cttgaaaaag aaaccaagcc accacaacct tataccgaag
      181 gtacgttgat cactgcaatg aaaacagctg ggaaaaccgt ggatagtgaa gaagcgcaat
      241 ccatattgaa agaagtggaa ggaatcggta cagaagcgac acgggcgaat attattgaaa
      301 ccttgaaaca aaaggaatat atcaaggtag agaaaaataa gctcgtggtg acgaataaag
      361 ggattttgct ttgccaagcc gtggaaaaag agccgctgct tacgagcgcc gaaatgaccg
      421 ccaaatggga aagctacttg ttaaagatcg gtgaacgaaa aggaacacaa accacttttc
      481 tagccaatat ccaaaaattt gtctctcact tattggaagt cgtaccaggg caaatccaat
      541 ccactgattt tggatcgacg ttacaagaag tgaaagcagc cagtgaaaag caagaagccg
      601 cgcgtcattt aggaatttgt ccaaaatgcc aggaacaaga agtcttactc tatcaaaagg
      661 tggcagcttg tacgtctgaa gcctgtgatt ttaaaaggtc agaagggttc atttgaagca
      721 acgattgttt taaaagaaga cttcactaca ggctttgaat tttctgaaaa gaaaaaaacg
      781 aacttcaaga aaagaacacg ccgcactgca aaataatgga gggttaatct tatgcgtaat
      841 accacacaca aacaacaaga aatcgaaaat caaaaagcca aaattcgttc agaacaggaa
      901 aaaatgcgtg ccagcaaacg aagactgcag caattgaaga accagcagag gattcaaaaa
      961 gaaatcgaat tagatcgata aaatcttcct aactattgta tacgtataaa cttatgttat
     1021 actgttttta taagtttata cgtatacaaa ggagggagaa aatgacaaaa gcaatggtat
     1081 acaagctgct agaagaattt aatggaactt tggctttgaa ggatgccaaa aaagcgggca
     1141 tttctcctgt aacgatcaaa cgtatggtag atcgagggga gctggatcga gagtatccag
     1201 gttttttcac cttacctggt caatttcctg atgaattgtt tatggcgcaa aaaaaatatg
     1261 aacgcggcat tatttcacat attaccgcgt tagatttgta tgatttaaca gatatgatcc
     1321 ctagacagat cgatctgact gtaccttacg gttatcacgt ttctgaaaaa ggattgaagg
     1381 aattcgcagt tgagctacac tatacaaaag cggaatggta tgaattaggt aagattgaaa
     1441 taaaaagtcg ctatgggaat cctattatag cttatgatcc agaaagaact ttatgtgaca
     1501 tttggaatcc ctggtatacc gttgaggatg aaatcaaagt aaaagcaatc aaaaactata
     1561 tggagtcgga cagaaaaaat cttagaaaat tgaatgagta tcgacgaatt ttaccaacag
     1621 acaaaacgat gcgttcttat attatggctt taaattaaag gagaagatac atgacaccta
     1681 cgcagttaaa agcaaaagtg aagaatattt ctagagaaaa agaagttgat ccgcagcttg
     1741 ttttaagaca ttttatgatg gaaaaattct tggaaaaaat ctcagagtct ccttatcgtg
     1801 aaaattttgt gttaaaaggc ggcttcttga ttggctcaaa gtatggcatt gaaaatcgca
     1861 caaccaaaga tattgatacg actttacggg aaatgaaagt aaccaaagaa acattaacaa
     1921 cggttctaaa cgaaattttt tcaacgccga cgaaagaaga cattcaattt gagattcaag
     1981 gaatgaaaga aacgagagaa gccgattact atccaggatt tagcttacgt gtgctggcac
     2041 atttggaaaa catgcggcca gattttaagg tagatgtgac tacaggtgac tcgatctatc
     2101 ctgcaacgat cactcacagt cataaattga tgtttgagga tcgaacgatt cctttggaat
     2161 cctatcctac agaacaaatt attgctgaaa aattaagtgc aacctttgat tttttaacag
     2221 ataattcgag aggaaaagat ttttatgatc tctatactat tcctaaaatg gagaagatcg
     2281 atgagaagac cttgaaagat agtgtgagaa atacatttac acgtcgagga aaaactgatc
     2341 cgttaaagat atactatgaa aaggatatgg atgtactgaa aaatagtcac gagctaaaag
     2401 atacctggaa gaaatatcaa aaaagcaatc cttatgctgc gtcgatcacg tacgatgaga
     2461 caatggattc gattagtaat ttgatggaaa aagtgattca cgtcgaagaa aaagagcgaa
     2521 aactagctta tcaaagacac caacagatga atagagaaat ggagagataa agatgattaa
     2581 taatgtgaca ttagtaggac gcttaaccaa agatcctgat ttacgttata ccgcaagtgg
     2641 tactgcagta gcgactttta ccctagccgt caaccgaaat ttcacgaatc aaaacggcaa
     2701 ttgagaagcc gattttatta actgcgtgat ttggcgcaaa ccagcagaaa caatggcgac
     2761 attagctaaa aaaggtagct tgattggtgt agttgggcgg attcaaacgc ggacttacga
     2821 caaccagcaa ggacaacgtg tatatgtgac tgaagttgta gcagataact ttcaactatt
     2881 agaaagtaaa gcagcgacag agagccgtgc gcacgctgat caatcgtcca ccagtccaag
     2941 caccgccacc tttgaacaac ggaatacagc gacgcctaac aacaatggat tgaacgcttc
     3001 acagaatcca tttggtggac aatcaatcga tattagcgat gatgatctac cgttttagtt
     3061 tgagaaagaa ggagtaagca tggcaaaaaa attagtctga cgatggatct attgataccc
     3121 gtaattgcat ttatagacgg gatttctttg ggcgtcactg aaaatccttt ggtaaataga
     3181 attgcgaaag gatcactatt tcttgtattc gtgtatcttg tctgttctta tcttttaaaa
     3241 agagcaggaa aggataattc cttttggcga ttgactaaag tcacagtagc agcgacagct
     3301 gttatactta cggctattct accgctttat atgtagagtt aacgggaaat cgttgaactt
     3361 cttgcgaaat attgctataa tgaacatgaa aaagaggagt tgcgctaaca acccctcatt
     3421 aacagcaccg attaaagaac ggtggcagaa tattagttat ttggaaataa ccgctgacct
     3481 gctaaagtag acggttattt ctttttgtca tttttaagca tatttacaat caaactaatc
     3541 aaagcaatgg taaacatacc aaaaccaaga atggtttgaa ttgtctcata tgcggacaaa
     3601 aggcttactc ctttcataga ttttagcact tacattcata agcaccacca cctttccaga
     3661 agtcgccacc gtcttcaaac tttactgtct tgactattat agcatatcta aaatcgaata
     3721 aaaattctca attttagctg tttttgctat aattttagcg aggtgatcgt gatggaacaa
     3781 tatattggtt tactcgataa attagggtga tcaagacaca gccattgctt gtgcgattta
     3841 cattacgcac gagtcatgaa ccaatcaatt gtgtagtggc caatatagaa atagcggata
     3901 aattgctgat tttggaagaa ggcaagtata ctatttcagt gacaggtcac ttcaataaac
     3961 gaaagcaact agtcattgaa tcaatgcaaa ttagaaaccc tgatcatttt acgcgatcga
     4021 tgagcatata aaagaagagc tgcagtccta aatgaggatt gtagctcttt ttgttttaca
     4081 tagaaacagt aaaaatgaaa aggcctgttt aggaaggcac aagcgccttc tcaattgaaa
     4141 gacctttctc tcactccaac cgccaccgcc gttcgagaag aaaggaagtg aacgctacgc
     4201 gttcactaag ataaagggtc ttgcatagca agacaaaata gaaaaaattt cctctcacca
     4261 aaaggttcgc tcctgttcgt tatctcttta ttgatttgtc agaccggcaa caagttgcct
     4321 tttatcacca aaaagcgaca aggacaaggg taaatggcac gctgcgcgcc cttgtcttgt
     4381 cgcttttcgt tgattcattc tggccaacaa atcaataaag aaagaactaa cgaaaggaag
     4441 taaaaccttt cctaagagga ggcagaatca atcatgcgaa agaaatttga ttattggggt
     4501 gttcccttca gcgaattatt cccaaactat catgcaccac atactgtcga atgcgattgt
     4561 ggagaaagag cgaaatgtat caaaagctat tgtttgtatc aatgtccaac atgcgggaaa
     4621 aaatatacct tgtcttatgg cgattatatt ttaattgatg agaaaggaaa gaaacgctga
     4681 tgaaaacaca acacaaatta ttgactgtaa aagaagtcgt tcaaattttg gtggacaaag
     4741 aagtcatttc tgaaaaagta gcagaagttt tacttattcc gcggtactgg acgatagaaa
     4801 cccctttagt agaggatgca ccgactatgc tatttgaaga cctagtgaag attgcaggat
     4861 atgaactgca ttatcaagaa aacttggaaa cttatgaaaa cgaatattgg ttaaccaaat
     4921 tagagaacta aaaaggagaa aaaaatatgg ctcaaaatta ctatgacgaa tttgtcaaat
     4981 tacctttgga taaaatggca caaaaaatgg aggatatgac gttcctatac aacgaaacac
     5041 gtgttccaaa aaaacattat aaagaaaaat tgtcggtagc tgtggaagaa atgattgaat
     5101 aaggtgtcga aatgaatctt attgcgacgt actatcgaac actggaagaa ctgaaaaagc
     5161 aaaatgcaaa atggtttttc caagccttac tatgcttgga agtaggtgtc aaaccaagca
     5221 ctattaaacc ttctgaatac caagctttag aactaacgta tgcgaaattt atcgaaacga
     5281 aaaaagccaa gactgtttca agtgaatggt tagattattt tgaaaatatc aataagtatg
     5341 gcgcatatta caccatgaaa aaggaagaca acgaaaatga ataaggtgat ttatcaacta
     5401 acaacggatt ccatatcaac gatttgaaag aaaaaaaggt caataatcat ttataccgaa
     5461 aagtgagaat gtgtgaaaca aggacaaaaa aagcctagta actaaggaaa tggttcttat
     5521 ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa acgggcaaag
     5581 cccgataact taagagaaag aaggtgatcc atttgaacaa gattattccc ttattgttag
     5641 tactgtttgt tggcgaaata cttatgcgag atagtgcagc tgtacagtta aacctcgctg
     5701 tatttttggt aatactggtg ttttgtttga tcttttggcg gtttgtatta gtcctcgtga
     5761 gtctactatt caagatgatt gcagccatat ttttcatagg gttatttcga tgaaaggagt
     5821 gagtaccaat gccaattgca ataggaaata aacggttacc cgtgacatta gatgaaaaga
     5881 gacaaaaaga attgcagcaa ctaaagcaga agtatggcaa aagtgaatct aagattatgt
     5941 gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatgta ccagcgctaa
     6001 aaaagtgacg tcacttttta atttgatcta tataagtgat atcactttta ccaaggaggg
     6061 aattaaatgt taaaaactaa aaagaattcg tacaaattaa agcttagtat actatctatt
     6121 atttgtcttt taggcatgat aattagtggt catttgtata gagtggcata tacgcagttt
     6181 attgagtaca aggttcactc tttaggatta gctggggatc gatacgttga atatgtccat
     6241 tattcattaa aatgttcttc atatattctt ctttggctta ttttttcatt gtttgaattg
     6301 ctaaatttaa tagcaatatc ttcatatcac ttagataagc taaataccaa atctagccgt
     6361 tataacgaag tgtttaagga aaatgtgaag ttagaaagtg acttaaacaa gctaaaaaaa
     6421 taaaaagaag aaaacataag ttaactaaaa ttattttatt aagaatgatg gaatataagg
     6481 ataaggttac caacataacg atcattactt ctctgatatg ctatggactt ctatcaatca
     6541 gtattttgtc tatagattat ggagtaacta tactgcaagc cttagttttg gctatgccta
     6601 tgtatctatc tttgtttatc atgcatgaaa tatataagct atttgtttta tcaaaaaatg
     6661 tagaaatacg ggtaaatatt ttggtgaata gttatcaaaa agaactcaaa gaaagaagac
     6721 ggtggcggta tttattgaaa ggatgaaaaa gtaggatttg acatactggt attcagaaag
     6781 tgccgtcact tttaaaacaa tggaaaaggt ggagataaga tgaagaaaaa acaaactaaa
     6841 ctatcattag cagatattta acacagttaa ccgcaactga agatggtgtt gttgaatttg
     6901 aacaatctga aattgcggta gtagatgatc gttaccttaa aatgccttac ttctttgacc
     6961 aagcaaaggt catttgcctt tgtcactacg atggggtacg tgattttagg cgttaaaaag
     7021 tagtgattat atcaacttta cgagtaaata taaatttaga catgaaactg aattatccaa
     7081 tggaaaatca tggtttataa tct
//
LOCUS       DACDYJ010000105         6980 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDYJ010000105 DACDYJ010000000
VERSION     DACDYJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.107"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            80..691
                     /locus_tag="IWM51_002572"
     CDS             80..691
                     /locus_tag="IWM51_002572"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9971935.1"
                     /translation="MRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSINQKW
                     VTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTPSEN
                     LIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYPSKA
                     YENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            926..1378
                     /locus_tag="IWM51_002573"
     CDS             926..1378
                     /locus_tag="IWM51_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971936.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1392..2081
                     /locus_tag="IWM51_002574"
     CDS             1392..2081
                     /locus_tag="IWM51_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971937.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2206..4557
                     /locus_tag="IWM51_002575"
     CDS             2206..4557
                     /locus_tag="IWM51_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP9971938.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            4637..5032
                     /locus_tag="IWM51_002576"
     CDS             4637..5032
                     /locus_tag="IWM51_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971939.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5357..5974
                     /locus_tag="IWM51_002577"
     CDS             5357..5974
                     /locus_tag="IWM51_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9971940.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6015..6314
                     /locus_tag="IWM51_002578"
     CDS             6015..6314
                     /locus_tag="IWM51_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971941.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6348..6515
                     /locus_tag="IWM51_002579"
     CDS             6348..6515
                     /locus_tag="IWM51_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9971942.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(6560..>6980)
                     /locus_tag="IWM51_002580"
     CDS             complement(6560..>6980)
                     /locus_tag="IWM51_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9971943.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2387 a         1033 c         1386 g         2174 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc caaatactga tgagaaaatt aggcttgcgt tcagtcaccc tcaagaagta
      121 caagcctggc aaacaagcga aagttaaaag tgaaggacgt aaaaacctgt taaaccaaga
      181 ttttaccact acgtcgatca atcaaaaatg ggtgacagat attacctata ttcataccct
      241 aaaagatggc tggacctacc tctccactat tcaagacctc cacacaaaga aagtcatcgg
      301 ttggaagttt ggcaagcaga tgacgaaaga attggttatc gaaacccttg acaatgcgct
      361 cttaaaccaa acaccctccg agaacttgat catccactca gatttgggtt ctcagtacac
      421 tagcgaagcc tatgaggcaa agctgaatga attgaacatt cggcactcct tcagtcgtaa
      481 gggctgtccc tacgataatg ccggtatcga atcgtttcac gcctcactca aaaaagaaga
      541 agtctatcca tcaaaagcgt atgaaaactt tgaagctgct tatttagcgt tatttcaata
      601 cattgaagga ttttacaatc gcaagcgaat ccatagtagt atcaactatt taacaccaaa
      661 tcagatggag gagttggcat tgcaagctta gttcctagct accacttaca tttttttggc
      721 gagatttcca cagggtttca acttcatcgc ttcgatcagc ggtcaaacgg ttttggtttg
      781 accgctggtc gaaacgatga gatactcacc cgctggaatc tcgaaaaaat tctcacaaat
      841 cgtgtccgag gtattgactc aaatccacca attatcttat cgtagatata tattataaat
      901 ttaataagaa agcgaggtga gaatcgtgaa aaaaacgata ctaagtattt tttcagtatt
      961 tacaatatta attgggggct attttctttt gaatttagat tctgttaatt caaacgctca
     1021 aataaaagat caaagagtag ctgaacataa taataaaaaa atgagtttga ccaaaaatat
     1081 ctattctatg tcagatgaag aattgtataa agaatttggt gatttatccg gggaacagtt
     1141 agaagaaagt attccaggaa ttagtgactc agataaaatt gttgtcttgt ctgctggtgg
     1201 gcaattaatg ggagatggca aatccgaatt agttagttat aaacaaaatc acagacagtt
     1261 tattgatgtt aatactgatt ccaacgctat ttcgattaag gatctaaaag agcttttaaa
     1321 tagtaaaaga aatgaattaa agcagataaa agaaactaat tcagtggaat tagagtaaag
     1381 gaagattcat tatgaaaaag atttttggtg tttctgttgg gctacttatg atgttaacat
     1441 ttggaaatgc agtatatgct gctgagaaag aagttatttc agatccagag cagtacgcct
     1501 cacgatactt tgctgatttt gaagaagaag ataaagtact tgcattgcca gaaggtggtt
     1561 ttttaaaagg ggaagctaaa attgtatcta tggataatcc agatataatt caaggttcat
     1621 ataattcaga aactgatcct gcttcagttt cagtgacaga agcaaaaaaa attgttgctg
     1681 aaaaattcaa aaatgatatt gaaactactc caattatgcc tcgaggagca tttccacccg
     1741 atgttggtac tagcggacac cttcttgtac tagcaggagg aggaagctat aaatcacagc
     1801 catttagtgg tgcgggatgg cgttttggag gtatgggtgt aaaaccagct gatggtacag
     1861 gcaacttttt acgttggaaa tcctacattg acgatggaag agtaggaaat gtaaatcaag
     1921 ccaacgcaac taaatctggt tctattcaag gaatggcatt gtatgctcgt gaaggattca
     1981 aatactttgc accaaacggg cctggttgga caatgaatat aggtatttat tacacttata
     2041 atcctgttgc aggtacgtat tatgttgttc aaaatattta ataaaacagc acaaagagat
     2101 ttctttgtgc tgttttatta tttttagttg ttgacaaaca ctaaaatata aaatataatc
     2161 aacacataaa cgaacgtatg ttcggcaaga aagcagggga tttttatgga tgagatggtt
     2221 ttaagtactc agaaatggct aaacaagaaa tacagtaatg ttacagggtt tgataaggtt
     2281 ccagaaaatg ggcgaacagg gtggcctaca atctatggac ttattgaagg actacaagta
     2341 gaacttggta ttactaatct agtagctaat tttggaccta ccactgaaaa aatgtacgat
     2401 aatcaggtta ctcctaaatg gggcaaaaat ctacctaaaa atattgtatt tcttattcaa
     2461 ggtgcctttt ggtgtaaggg gattaatcca ggtggcttcg atggggtata tactccatat
     2521 ttagatactg ctgtaaaaga gttgcagact gatgcagggt ttaatggaag cgctgtaact
     2581 ggagttttgg atgcgaaatg ggccaaagcg ttatttgata tgagtgcttt tgttcttgtt
     2641 cccggcggag atagtaaaat tagaaaaatg cagcaatggt taaatgtaaa ttatttagaa
     2701 tacacgggaa taatgccttg tgatggtatt tatcaaagaa attcaaatca agcattaatt
     2761 tttgcattac aagctgaact aggatattct cccagtgaag caacagggtc ttatggtaat
     2821 gggacaacta gtaaaactta tcctgtgtct gaaggtaact cgggaaacta tgtaagaata
     2881 attcaatatg gattatatgt taatggttat tttgaagacg gtacattcga tggtattttc
     2941 actaaatata tgggtttaga agtactggca ttccgaaaat ttatggtatt gcctgaatat
     3001 actgaaatag cagatgttgt tgtaataaaa ggactattgt ctagcgctgg agatattaga
     3061 agatctgcag atggcgcaga tacttctacg caattaacta gatctcaaat ccaaacattg
     3121 gttgataatg atataaaaat agttggtaga tatcttactg gtactgttgg aattggaaaa
     3181 gatgaacgaa ataagtatct aactacagag gaattgaata atattttttc taaaaattta
     3241 tctgtgtttc ctatctatca agatggaggt gcagcattag cttattttac ttatgatcgt
     3301 ggcttatcag atggtaagaa ggctatagat gcagcaaaaa atctaaatat accattaaca
     3361 acagttattt attttgctgt tgatctagac atgttagggg aagaaattct tgcatatgct
     3421 ggagaatatt ttaagggggt atctgctgta atgtcatata gcgggtatca aacgggtgtt
     3481 tacggaacaa gaaatgtttg ttctcaaata atcaataacg ggtatgcttc gtttgcattt
     3541 gtttcagata tgtcgactgg ttatagtggg aatttaggtt ttcctatgcc tagacaatgg
     3601 tcttttgatc aatttgttga atttaccata ggaagtggca acggagctgt tggtatcgat
     3661 ttaatcgcta catcgggtag agatagcgga tttaatgagt tatcaaatga tactaataat
     3721 gataattaca ttgccaaata caatcagaag gttataaatc agatggtacg tgcgtatcaa
     3781 tatctatcac aagctggtct tgataatcct tggaaccctc atttgacatt ttatagatat
     3841 gtaaattatt ctggtttgtc atgggacatc atttcttctc ctgtaacaga gcatgataga
     3901 aaaatttatg atgaatatag gaataaatta actaatagcg agggcctgta taattatttt
     3961 atagatccta atactggttc aataataggt ttgcctcact taattgtgac gttacaatca
     4021 cagatgtttc tagccgacac aatcaatgat agtgtttctg actttgcagg ttggttagga
     4081 gatttaatga cttgctgggg tgaagttaag aaattaggaa tacaaatgga gcaaggtgta
     4141 tttgcattag taggtacggc tgcaggtgga acatttagtc tagaagattt ataccaagat
     4201 gttgacgcgg taaatgttta taccttagca aaaagaaatt ctgattcaac agctttatcg
     4261 gctttaaaaa cgtactattg tgataagggt gctattgata gatttaatca atttgttaag
     4321 aacagatttg gttcttcaga taaaatcgct tcagtaacaa atagtttatt agaaaatgac
     4381 gaaacagttg atatcttagg tgtagggata atttcatttt tcattgataa aatagttaat
     4441 aattcacttg ggacagattt tacttatctt gattttattg gagatgaggg gaaagttgca
     4501 aatggatttt ctgaaaaaat tattcaattt caagaaaata gaagtaaact taattaagaa
     4561 tcgtttacta tttaaacagt gtccttttcc tttttaaata tatacgataa aataagtata
     4621 tgaattgtaa aaggacatga tgattatgga agaaatagaa tggaataatc tgactcaaaa
     4681 taataaaaat aattctggat gtggaattat ctttatgttt gttatagttt tgggaatagt
     4741 agtactttta ataactgtca agatagtaga ttactttgcg aatactcctg aagaacaaat
     4801 acagagaaaa cttcctgagt ttgaaagagt accaccttat gatccaagag ataattcatt
     4861 tcaagtaaaa aaattaaaag agtctccaaa tgaaatgtgg tttgaagtta cagttgaatc
     4921 aagcgacttc aaagaaaaaa agaaagaata caattatatt gtaaaggcaa aagtatcaaa
     4981 aagatttatt tttaacccta aattagaaat atatagtgtt gtaatagaat aatagtgtta
     5041 aaaaaattgt aaaagttaaa gaagtcaaat tctgatgtga aaaataaaat ataccatgga
     5101 agacataaaa gaaaaactgg ataactagtt tatttaatgg cagaccgtta aggcctgcca
     5161 tttttatgta ttttagcgga ataaatataa tcatgcggga gtgtaaactt actgtttcaa
     5221 aaagtatact ttttgacatg ccttgatttt ctttgtttat gtatggcata ataaccacat
     5281 aatacaattt gaaacgtttc tgatttaatc tgattgtttt gaaacaattt tagtgaaaaa
     5341 agggtagata aaaacgatga gaaaattttt ttatatccgg gtcagtagtt atgatcaatc
     5401 gatcgaacga caactgattt ccgcgaagga attaggaatc cctgaagaat atatatttgt
     5461 agaaaaagcc agcggcaaag attttaaacg ccctgaatat caattaatga aacggatgtt
     5521 tcgaaaagga gatttactct atctacaatc attagataga ctaggcagaa ataaacaaat
     5581 gattttagaa gaatggcgag aattaactaa ggaaaagcag attgatattt ctgtgttaga
     5641 catgcctctg ttagatactg ctagttacaa gcaactagat gggttagaga cgcttatctc
     5701 tgacttaatt ctgcaattac tcagctatat ggccgaagac gagcggaaac gaattaatag
     5761 ccggcaaaga gaaggcgtag aagctgcgaa acaaaagggt gtaaaattcg gcagaaagaa
     5821 aattgcttta cctcctgaat ttcctgcgat ctattctgac tggaaagcag gaaagataac
     5881 tgcagtctat gctatggatc aattgggcat gaaacgaaat accttttatc gacgggtgaa
     5941 agaatatgaa cataccttgc accacttttc ttgatagata taacagctat tagacgggaa
     6001 gggagagttt gaaaatggac ttaacgaatc aaagggtcgt ttttactgga acgctgcagc
     6061 aaatgacacg aacccaagcc atcggtttgg ttcactcttt aaacggccat tgtcaatcaa
     6121 gtgttacttc aaaaacaaat ttcctggttt gtggtcagta ttctaagtcg ttgtttgatg
     6181 attctctcta tacaaaaaag gaacaaaccg cgcgggactt gattgcgtta ggtcatgaga
     6241 ttaccatttt atcagaagtc gaattttatg cgttaattag tcaacagttg aaagaatggc
     6301 aacggtattt ataatcgtta gtaaaattta caaggaggaa aatttttatg aagcgaaaat
     6361 cttatgatcg ttggggcatt gaaataacta gttttaatcg aaatattgct tttcacgtgg
     6421 agtgtgactg tggggaatta gcaaaaagtg tttatcaaaa aaattacttt tactgtccta
     6481 aatgccatcg ccgatacggg gtacatggaa catagaaacg aaatagtgcg gtagaaaagc
     6541 gaatgaatgt agtatatttc tacaaatcat tcgctttctt tattttataa atagttggtc
     6601 gagagatccc gttttctttt gcaatctctg caactggaat gccttgcttt aacatgtcca
     6661 ccacggtttt aaaaactaag cgtttttgcg gatctgttga gttaatagaa tactcgattg
     6721 gtcgtccctt atatcggccg gctttttgag ccaataatac tccttgccgc tgacgttcac
     6781 gaattttctc acgctcgttt tgtgcaatat aacttagcag tgataaaaac atatcgaaca
     6841 tagcggtatc caggagttca tttccagtat taaaatctaa aaactgtgca tctaatattt
     6901 ttaacgctac ttttttctgt ttcataaaag caactgtatt ttttatatct tcataatctc
     6961 ttcctaaacg atctagtgat
//
LOCUS       DACDYJ010000106         6652 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDYJ010000106 DACDYJ010000000
VERSION     DACDYJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.108"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            38..127
                     /locus_tag="IWM51_002581"
     tRNA            38..127
                     /locus_tag="IWM51_002581"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72..74,aa:Ser,seq:gga)
     gene            142..213
                     /locus_tag="IWM51_002582"
     tRNA            142..213
                     /locus_tag="IWM51_002582"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:175..177,aa:Glu,seq:ttc)
     gene            229..301
                     /locus_tag="IWM51_002583"
     tRNA            229..301
                     /locus_tag="IWM51_002583"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:262..264,aa:Val,seq:tac)
     gene            311..383
                     /locus_tag="IWM51_002584"
     tRNA            311..383
                     /locus_tag="IWM51_002584"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Asp,seq:gtc)
     gene            419..494
                     /locus_tag="IWM51_002585"
     tRNA            419..494
                     /locus_tag="IWM51_002585"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:452..454,aa:Phe,seq:gaa)
     gene            496..576
                     /locus_tag="IWM51_002586"
     tRNA            496..576
                     /locus_tag="IWM51_002586"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:530..532,aa:Tyr,seq:gta)
     gene            583..656
                     /locus_tag="IWM51_002587"
     tRNA            583..656
                     /locus_tag="IWM51_002587"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:615..617,aa:Trp,seq:cca)
     gene            659..731
                     /locus_tag="IWM51_002588"
     tRNA            659..731
                     /locus_tag="IWM51_002588"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:692..694,aa:His,seq:gtg)
     gene            743..814
                     /locus_tag="IWM51_002589"
     tRNA            743..814
                     /locus_tag="IWM51_002589"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:775..777,aa:Gln,seq:ttg)
     gene            860..930
                     /locus_tag="IWM51_002590"
     tRNA            860..930
                     /locus_tag="IWM51_002590"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:892..894,aa:Cys,seq:gca)
     gene            951..1035
                     /locus_tag="IWM51_002591"
     tRNA            951..1035
                     /locus_tag="IWM51_002591"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:985..987,aa:Leu,seq:caa)
     gene            1097..1169
                     /locus_tag="IWM51_002592"
     tRNA            1097..1169
                     /locus_tag="IWM51_002592"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1130..1132,aa:His,seq:gtg)
     gene            1179..1250
                     /locus_tag="IWM51_002593"
     tRNA            1179..1250
                     /locus_tag="IWM51_002593"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1211..1213,aa:Gln,seq:ttg)
     gene            1268..1352
                     /locus_tag="IWM51_002594"
     tRNA            1268..1352
                     /locus_tag="IWM51_002594"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1302..1304,aa:Leu,seq:caa)
     gene            1607..2482
                     /locus_tag="IWM51_002595"
     CDS             1607..2482
                     /locus_tag="IWM51_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9971944.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            2586..3350
                     /locus_tag="IWM51_002596"
     CDS             2586..3350
                     /locus_tag="IWM51_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP9971945.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            3367..4779
                     /locus_tag="IWM51_002597"
     CDS             3367..4779
                     /locus_tag="IWM51_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9971946.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            4796..6121
                     /locus_tag="IWM51_002598"
     CDS             4796..6121
                     /locus_tag="IWM51_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP9971947.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            6134..6592
                     /locus_tag="IWM51_002599"
     CDS             6134..6592
                     /locus_tag="IWM51_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9971948.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         2100 a         1084 c         1471 g         1997 t
ORIGIN      
        1 ctcattttga gaaagagtat tgagcttctt tcactctgga gagttgtccg agaggccgaa
       61 ggagcatgat tggaaatcat gtagatggtg ccaaactgtc tcaagggttc gaatccctta
      121 ctctccgtag caaatttacc tggcccgttg gtcaagcggt taagacaccg ccctttcacg
      181 gcggtaacac gggttcgaat cccgtacggg tcattttaat tgaatgttgg aggtttagct
      241 cagctgggag agcatctgcc ttacaagcag agggtcagcg gttcgatccc gttaacctcc
      301 ataatgaact ggttccgtgg tgtaggggtt aacatgcctg cctgtcacgc aggagatcgc
      361 gggttcgatt cccgtcggaa ccgtcgttct tgttattcaa ttaataattt taaaatatgg
      421 cgcggtagct cagttggtag agcaacggat tgaagctccg tgtgtcggca gttcgattct
      481 gtcccgcgcc accatggagg agtagcgaag tggctaaacg cgacggactg taaatccgtt
      541 ccttagggtt cagtggttcg aatccactct cctccatttt ataggggcat agtttaaagg
      601 tagaacagcg gtctccaaaa ccgttagtgt gggttcaatt cctgctgccc ctgccatggc
      661 gaatgtggcg aagtggttaa cgcaccggat tgtggctccg gcattcgtgg gttcgattcc
      721 catcattcgc cctttttatt cttggggtat agccaagcgg taaggcaagg gactttgact
      781 ccctcatgcg ttggttcgaa tccagctacc ccagtttcta taatttattc tttattttat
      841 agattgataa tagtataatg gcggtatagc caagtggtaa ggcagaggtc tgcaaaacct
      901 tcatcaccgg ttcaaatccg gttaccgcct tagtactaat aaatcaactt gccggcgtgg
      961 cggaattggc agacgcgctg gactcaaaat ccagtgcccg ttgagggcgt gccggttcga
     1021 ccccggccgc cggtattgac taagatgtct tcatcttagt tttttattga gaaattaaat
     1081 aacatctttt atgatggcga atgtggcgaa gtggttaacg caccggattg tggctccggc
     1141 attcgtgggt tcgattccca tcattcgccc tttattcttg gggtatagcc aagcggtaag
     1201 gcaagggact ttgactccct catgcgttgg ttcgaatcca gctaccccag ttattatttt
     1261 taaatttgcc ggcgtggcgg aattggcaga cgcgctggac tcaaaatcca gtgtccgttg
     1321 aggacgtgcc ggttcgaccc cggccgccgg tataattatc aatatagcaa ctttcttata
     1381 gagagttgct atattttttt tgtttttatg taattacctc caaatcaatt cgatacaagt
     1441 attatcataa aacgagtaaa aaagatattg ataacgcttt tattatgtgt taaactattt
     1501 ttgtaaatta tgtagggtgc tactattcaa ttaggcacga cctaagaaag ggttctttct
     1561 taggtcgttt tttgtcttta aaaattgttt ggagacaaaa atatgtatga aggagtctgc
     1621 aagttgtttt gttttatcat atagaggagg tgaattaacg gtgtatgtga tgaaaaagct
     1681 gaatcataat gctgtattga tcgatgacga tggaatagaa aaaattgcga tggggaaagg
     1741 gattggcttt tctgctaaag tgagtaagcc atttgatgtt gaggatgctg acaaattatt
     1801 tgtattagat tctaaagaaa agatcaaacg attcagtgag atagtctcgc agattccaat
     1861 cgaatttatg gattttgcgg aagaagtgat tgaactgata acagctagga tctcctctcc
     1921 attagattca aatatctata ttacattgac agatcatatt tattttgcta ttcaacgaaa
     1981 aagagaagat agagatgtca cagcaattat gttgccagaa atgagattcc tttatccaaa
     2041 tgaatatgat atagcagtag aagtggtcga gcgaatcaat catcgatatc aaaccgagtt
     2101 agggtcaaat gaagtaggat tcatcactat gcatatcatt aatgctgagc taggagagcc
     2161 taatagttta aagagcttaa aaatagtaga aatgacgacc acgattttgt cccatctaga
     2221 aaaatcctat tcaaacgttt atgataaaga atcactgact tatcatcgct taatggttca
     2281 tatcaaattc ctggttaaac gcttgattta tcaggaagaa acgccagtag aaaatattgg
     2341 tttttttact gaaacattta aacatagcaa agcgtattgt acagcactaa agattaaaga
     2401 tatcatcgaa cagcaatttc aacttgccat tcaagaaaat gaaatcatct atttggcaat
     2461 tcatctagca agaatctatt aaaataaggg cgtaactatc tataggcgca accaaatgac
     2521 acatagtaaa tggactatga ttatttggtt gcgcctattt tagtagaaaa aagaaggaga
     2581 atgaaatggg aaaagtgatt tttaattcgg atgattttgg ctatagccat ggtgtgaatt
     2641 atgggattat ggatgcttat cagcgaggaa ttttgacatc aactacattg atggcaaata
     2701 tgccgggttt cgagcatgct gtgaaactaa gaaaggaaat gcctaggcta ggtgtaggag
     2761 tccatttaac attgacgtgt ggcaaaccac tcctcaaaac tgtcgatact cttatcgaaa
     2821 gcggagagtt cagatcctta tcttattatc agcatccatt taacattgac aaagatcaac
     2881 tgtatcagga atggaatgct cagattcaaa aaatttatca tgcggggatc ataccaactc
     2941 atttagacag tcatcatcat actcatactt tggatgaaaa tcaggaggta gttgttacat
     3001 tagcaaaaaa atatgatttg ccggttcgga gcaactttga acgaaaagat gaagttcgtc
     3061 atgtcgatta ttttgagcct tgtttcgatg tcgtaggaga aactgatgaa aaaaaacgac
     3121 aaatcaatac gagtgtggaa gaatatttgg caagtttact catgatattg agagaagata
     3181 aaacgatcga agtcatgtgt cacgcagcgt atttggatca ggaattgtta aatggttcaa
     3241 gttttgtgta tcctcgtacc caccaagtcg aatttttaat tcattcagca tttgctcaaa
     3301 aagtgaaaaa tgatcctttg attgagttag taacatatga ggatatttaa tagaggagga
     3361 ataaaaatga atgttaaatc aaatgtgcag aagcttggaa gagcgatgct acttccagtt
     3421 gcagcgatgc cattagcggg attgatcatg cgtttatcag ctgacgatat gctgaacatc
     3481 ccagtaattg gtgcagctgg aaatgccgtt tttggaaact tggatatctt atttgctata
     3541 ggagttgcaa tcggttttgt caaaaataag gacaaaggaa tacctgcact tactggtttt
     3601 ttagcgattg ctaccttgaa aaaaggctta gaaattatgg acccagaagt gaatatgggg
     3661 atttttggtg gaattatttc cggtttagta gcggcatgga cgtataatcg ctttaaagaa
     3721 caaaaattac cagtagtttt ttcttatttt gcgggcgaaa aattcccatt gactatggtg
     3781 atgattctac aaacaatcgc agctgttttt ttgggcttta tctggccgct catccaaatt
     3841 gggattgata attttgcaaa actgctcgtt gatatgggag cattaggtgt ggggatattt
     3901 atgtttttga atcgtttgct gatccccttt ggactgcacc atgtgttgaa tacgtatatt
     3961 tattacgatt taggaagcta tacatctcct agcaaggaag ttttcagagg agagatgaca
     4021 cgctttatca acggagatcc tcaggctgga ctgtttttat caggtttttt tgtagtaatg
     4081 atgtttggta taccagctat atgtctagct atctataaag cagcttataa agaaaataaa
     4141 gagatggtca aaggaatcat gggaagcggt gcaggaactt catttattgc aaatatcact
     4201 gagcctgttg aatttagttt catgtttctt tctcctatgt tgtacgtgat ccatgcactt
     4261 tttgccggtt tagcagggat tgtctgttac ttattcaata tacgaattgg ttttacattc
     4321 ggagcatgta ttgttgatta tctcatcaat tttcgaatcg ctaccaatgc tattttgatt
     4381 cttccaatcg gaattttctt ttttgccttg tattacgtta ctttttatta tttgatcaat
     4441 aagagaaaca ttcagacatt aggacgtgaa gctaaggcag agtttggcaa tgaagtaacc
     4501 ttagaagaaa cagagttagg gttagcaagt aaaaattatg aatatatggc aacaaaaatg
     4561 ctacaagcgt ttggaggaaa agccaatatc ctggatgtct atagttgtaa tacacgccta
     4621 cgggtagagg tagtggatcc caccatggtc gaggaacagc gaatcaagca attagggata
     4681 agcgggatca ttaaaccaac tgaaaaaaat tatcaaatta tcatcggctt agaagtgact
     4741 tatgttatgg cagaattcaa taaattatta gaagaataga aaggaaaaga tgatgatgaa
     4801 aaaacagatt atcactatag ctggaggcgg gagtacgtat acaccaggaa tcgttcaagc
     4861 cattttgaac aatgaaaaac ggttgccatt atctgaaata cgtttatatg atattgatga
     4921 agaaagaaat atggatatgt acttgattgt taaatttatg ctgaaaagaa aaggtttttc
     4981 aaatattcgc attcgtgcaa cggatgatcc aaagcttgct tttacaggat gcgattttgt
     5041 tttctcacaa attcgtgttg gaggattgga gatgcgagaa aaggacgaaa agattccact
     5101 taaacatgga ttagtaggac aagaaacgtg tggactcgga ggatttgctt atgggatgcg
     5161 ctcaatcaaa ggtttattgg aaattgtcga tcatatacaa gactatgccc ccaaagcatg
     5221 gattttgaat tatacaaatc cagagtcgat cgtttctgaa gctgttcgtc gtaaatatcc
     5281 gaatgtaaaa atgattaatg cctgtgatat gactatttca attgaagaaa cgattgcagt
     5341 gaattacggt tatgatcgta agaattggat cgttacgtat tatggtttaa accactttgg
     5401 ctggtataca tcgatttatg ataaagaatt gcaacgagaa attatgccag agattattga
     5461 aaaactaata acaaaagaaa tgcaagtcgc cgattttaat ataggagata aaacttggca
     5521 aaaaaccttt caaatgatgt cagtcatcac gaaaaatttt ccaagtaata tcccgaataa
     5581 ttacttagag tattacttat atcctgatat ggtcgttgag catactgaca aagagtatac
     5641 acgtgctaat atggtcatgg atggacgaga gaaaaatacc aaagaaatgg ctgataaaat
     5701 acgccgcggt attgtggaag aagtattgaa ctttaatttt ggggagcatg gacaatatat
     5761 cgtcgatata gctatttcct tgttgaatga tgatcgtcgt cgctttatgc taatcgttcc
     5821 taatcaaggg gcgattccga atctacggtc agatgcagtt gttgaaatac cggcatatgt
     5881 tggtgcaact ggtgtggagc caattacact aagaaaacca atcagtgatt ttcataaagg
     5941 attgatggag gcacaagttg cagtagagaa gttactagta gatgcttact ttgaaggttc
     6001 ttatcaaaaa gctttacaag catttacatt gaatcaaacc gtaccaaatg ccagagtcgc
     6061 aaaaaaaata ctagatgaaa tgatcaaagt aaacaaaaaa tattggccag cattaaagta
     6121 gaggtgataa aaagtgcatt tctttaaaac aagaaacatt ctaaaagctg tagctagtgg
     6181 tcaaattatt ccattaacag cagtaaaaga cgaggtcttt tcttcaaaaa tgataggagt
     6241 tggatacgct ataacgaatc atgatggata tatccatgcg ccagttaaag gaataatcga
     6301 taatatattt cctacccgac atgccataac gatcgaaagt gaatctggca gaaaaatact
     6361 gatccatatg ggtttagata cagttgaact cgaaggaagt ccgtttcaga tacgggttga
     6421 aagtaaccag gaggtaaaag aagggacact gttagctatt atagatttga agaagttgga
     6481 gcaagcacaa aaagatacgg tgatcattgt agtttctcca ggcatttttg aaggaaattt
     6541 attaaaacaa aaccaagaag taaagattaa agatagttgt tttgaatttt agtgtacgat
     6601 gaaaaaagtt aaatagggtg atatttatgg gtgaatttac aaaatttttt tg
//
LOCUS       DACDYJ010000107         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDYJ010000107 DACDYJ010000000
VERSION     DACDYJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.109"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IWM51_002600"
     CDS             725..811
                     /locus_tag="IWM51_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9971949.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IWM51_002601"
     CDS             complement(932..1621)
                     /locus_tag="IWM51_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971950.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IWM51_002602"
     CDS             complement(1628..1906)
                     /locus_tag="IWM51_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971951.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IWM51_002603"
     CDS             complement(1866..2780)
                     /locus_tag="IWM51_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9971952.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IWM51_002604"
     CDS             complement(2762..3142)
                     /locus_tag="IWM51_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9971953.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IWM51_002605"
     CDS             complement(3592..3879)
                     /locus_tag="IWM51_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9971954.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IWM51_002606"
     CDS             complement(3899..4123)
                     /locus_tag="IWM51_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP9971955.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IWM51_002607"
     CDS             complement(4763..5296)
                     /locus_tag="IWM51_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9971956.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IWM51_002608"
     CDS             complement(5317..6159)
                     /locus_tag="IWM51_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9971957.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACDYJ010000108         6124 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDYJ010000108 DACDYJ010000000
VERSION     DACDYJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.110"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..189
                     /locus_tag="IWM51_002609"
     CDS             <1..189
                     /locus_tag="IWM51_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9971958.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(275..1369)
                     /locus_tag="IWM51_002610"
     CDS             complement(275..1369)
                     /locus_tag="IWM51_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9971959.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1558..2319
                     /locus_tag="IWM51_002611"
     CDS             1558..2319
                     /locus_tag="IWM51_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9971960.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="IWM51_002612"
     CDS             complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="IWM51_002612"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9971961.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3094..3849
                     /gene="fabI"
                     /locus_tag="IWM51_002613"
     CDS             3094..3849
                     /gene="fabI"
                     /locus_tag="IWM51_002613"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP9971962.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3959..4972)
                     /locus_tag="IWM51_002614"
     CDS             complement(3959..4972)
                     /locus_tag="IWM51_002614"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP9971963.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            5137..6057
                     /locus_tag="IWM51_002615"
     CDS             5137..6057
                     /locus_tag="IWM51_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP9971964.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1880 a         1214 c         1094 g         1936 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatggattt tagaaaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgtaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcataac ctataaaaaa acaaaataaa aaagcagaaa taactggcta
      241 tttctagcgg attatttctg caataactaa ttatctattt tttattttca attaatttaa
      301 agcttgacca tggtttcaaa aagtcaagga ggaataattc gtcatcggag attcttccta
      361 caacattgac acgtccgtcg tttttcatat ccttcaaggc gatttgtgtt tctcctttgt
      421 attgaccgta cccttcattg tcaatcagta catccccatg atgcatatct cgcgtattat
      481 gaggcgggaa atctttatct ttgtagtata cacgagtcat cgttgaacga aggatatatt
      541 ctgagcgatc ccctcgataa ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg
      601 taataccttc ttccgtatct actttcaatg ttggataatc cgcgttaaat gcttctgcca
      661 tttctttcaa ttcttcttca cttgcgtacg catttccaat agaaatatca tcaatcaatc
      721 ctgtaaggat taaatgcttc acttgtgtcg caatttcaag atctcggtgg tcttccagcg
      781 tgcacaagcc atcttgcgta ggccaagggc caaactcagc agattgcgaa ttgacgaatg
      841 ccatcgtatt caaattatat tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc
      901 ctaaaccaga gtatcgatga ggataaaaat tatgagaacc taataaatta tccgtgtttg
      961 gcgaataact catgatgttg tcaacgtaat tcgtgcccga actcatattg atctcgattt
     1021 tgataccaaa aggattgcgc gtcatttttg cttcttctgc tcctgtgaat ccgatatcta
     1081 agcggacccc gtaggcaccc atcttatgga aaaaagataa gtcatcgtaa gaaatctcca
     1141 actgttcaaa taacgctgga ttgatatcaa ccattacttc catgcctagg ctattcgcgt
     1201 aatcaactac ttctttaaat tctgaaagaa ctttttcttt atcatcattg atctgcagca
     1261 aactggtaaa aacccgttta tacccatact tatgagctaa atccaagtac gctttgtcct
     1321 tttcaaatgt tgaccgttct ggataaatcg agattcctaa ttttcccata ttaccatttc
     1381 ctcctgcatc ttttcgtttt tgtatccact tacattatag caaacacaaa gtaaatggtc
     1441 tacacctttt ctttttcttt taaaataaat gataaattcc aataatttaa gtaggtattt
     1501 tccctctatt ttgttatcat taaagataac aaaatagtaa attggaaagg aacagttatg
     1561 aagaaaaata aagttatttt aagtattagt ttagcagcag cattgctgtt tgctgctggt
     1621 tgttctgcaa acaacacgac ttcttctagt tcgtctacag aaagttcaaa aagtacatct
     1681 actgcggaaa gcacgactgc cagtagcgtc gatttgaacg cattggaatt accacaattg
     1741 acgaaagatg tcgccgaaaa tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc
     1801 aaaatcaaat tatttcccga acacgcacca aaagcggttg aaaactttat gacacatgca
     1861 aaagacggtt actacaacgg actgactttc catcgcgtca tcaaagattt catgatccaa
     1921 ggtggcgatc ctaaaggcga tggcactgga ggggaaagca tttggggaga aggatttgaa
     1981 acagagatca acaaccacct ttataatata agaggtgcgt tatctatggc tcgctcacaa
     2041 gatcctaaca gtaacggcag ccagttcttt atcgttcaaa atacagatga tatgcatgat
     2101 ggtcttttaa aagacgacta tcctcaagca atcatcgacg cttataaaaa cggcggttat
     2161 ccttcactag atggccatta tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg
     2221 gatgctattg caaatatcga aacagatagt tcagacaaac caaaagaaga agtaaaaatc
     2281 gataaaatca atatactgca agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg
     2341 catagatgac aacgtatgct aggattcagg cgtcttcttt ttacataaaa agcaagtaag
     2401 cttccttttg ttttgctata tcaggtttta tagtcaagca ttttggttga cgatgaacgt
     2461 gaactcgcac tcacagactt ttttatcatc tacatatgct gcagataacg ctgtaccgac
     2521 tgatcccttc attttggtga tttcaaaatg acatttcagc acatctcctg gaacaacttt
     2581 ttgacggaat ttggccttat tgatcccacc gatataagct ctttttccta gaaactcttc
     2641 tgattttagg ataagtatcg aacctgcttg tgccagtgtt tctagaatca acgcacccgg
     2701 catcactgga ttgtcaggaa agtgaccttg gaaaaaatct tcattgattg ttacattttt
     2761 tgtcgcaacg atcttttttc ctggttctaa agtatctaca taatcaatat aacaaatagg
     2821 ataacgatta ggaatcaatt ccattacttc agtagcagat aatacgtaag acataattaa
     2881 actccttctt tcaggcaatt gccattttca ttactttgac tatcaaagta tcatattcct
     2941 aaatatttag caatcttttt ttaatttaag aaaaaatagt ttgacaatca aaacattttg
     3001 actaataatt aagaaatcaa gaagttattt tagctattat tctctatttt tttacgacta
     3061 tcaaattttt atcagaaaca aaggagagtc attatgtcat ttctagaagg aaaaaaaatc
     3121 gtcattatgg gcgtcgctaa caaaaaaagc attgcttggg gttgtgctga agcaatgttg
     3181 aaacaagggg cagaaattat ctatacttac caaaatgaac gaatgaaaaa atcattagtc
     3241 aaattggtcg gcgaagaagc attcactgta gaatgtgatg tttctagcga cgaaagcatc
     3301 gaaaaagcaa tgaatgccat cggtgaatat gctggggaga tccacggcct tgtccatgca
     3361 gttgcttacg ccaacaaaga agaattgtcc ggaaatgtgt ctgatatttc cagagacggc
     3421 tatcaattag ctcaagatat cagcagctac tctttaattg ctgtatcaaa atacgcacaa
     3481 ccgtttcttg caccaaacag cgggatcgtt agcatgactt atctaggttc agaacgcgca
     3541 atcccgaatt ataacatgat gggtattgcg aaagcttctc ttgaagctgc tatccgttat
     3601 ttggcagctg agttctcgcc taaaggaatt cgggtcaatg gaatctcagc tggggcaatc
     3661 aaaacattag ctgtcacagg tgttaaagac tatcaaaaac tgatccagtt atctgaatct
     3721 cgtacgcctg atcagcaagg tgtgacgatc gaagaagtcg gtaatgcttg tgctttctta
     3781 atcagcccac tttccagcgg aatcatcggt gatattatct acgttgacaa aggtgtccat
     3841 ctttcataaa cttttgttgc attctttaaa gtaaaacata taaaaatcag gaaaaaactt
     3901 tttaaagtct cttcctgatt ttttcttttt acgttttttt caactcatta ctttgaaatt
     3961 attccacgac ttcaatccag ccttctggtg cttctatgtc tccaaattga atacctgtca
     4021 attcatcata caaccgtttt gttactggtc ctacttccgt ttcactataa aatacatgaa
     4081 gattgttatt gtgataaatc ccaccgattg gagaaataac agccgctgta ccacatgcac
     4141 cagcttctgc aaattgatct agatcatcaa tgaaaatatc tccttcaatt gcttgcagtc
     4201 ctagtcgttc ttcagctaac tgcaacaagg aatatttcgt gatacttggt aagatagagg
     4261 gcgattgcgg agtgatgaat tttccgtctt ttgtaattcc aaagaaattt gctgcaccta
     4321 cttcttcgat tttcgtatgt gtggctggat caagataaac acaatctgag taatccagtt
     4381 cttttgcttc tgttcctgga agtaaactcg ctgcgtaatt tccgccgact tttgctgcac
     4441 cagtcccctt aggagcagca cgatcgtaat cagatacaat aaaattcgtt ggttttagtc
     4501 cacctttgaa atacgctcct actggcgtac agaagacgac aaataaatat tctggagctg
     4561 gatggacacc aatgttttca ccgaccccaa tcagtaaagg gcgcaagtat aatgtagcgc
     4621 ctgttccata gggcggcaca aaatcttcat ttgcttttac cacttgcttg acagcatcaa
     4681 cgaacatctt ttcagggacc attggcatca gcaatcgtct agcactttga ttcatccgtc
     4741 gactattttg ttcaggtcga aagagattga ccttgccgtc ttttcgacga taagctttta
     4801 gaccttcaaa acattgctga ccataatgaa gcgctggaga gccttcatga atcgtcaggt
     4861 aattatcttc tgtcaattct ccttcttccc attgtccgtc tttccatttg gcaataaaac
     4921 gccaaggagt tttgatataa gaaaaaccta gattgttcca atcgatttgc atatacttac
     4981 caccttcgtt attttctcca cattttatca ttatccatat tatttgtcat tttattttca
     5041 gcttttttcc ttcattatag ttatttttgc tatttccttt tttgaaaact tcttggtaca
     5101 atacagaaag aatcaaattt tagaaaagag gattttatgt tttcattatt agcaactgcc
     5161 ggtacgacag actcttctgg cgagacattg acagatcaag ctgtcagcaa agtaaatgcg
     5221 tttcagcggt tttgggacgg gattgattgg gacgccattt tggctgcctt tatccaaaaa
     5281 agtttatatc tgattttttt gatcgtcctt ttttggattc ttcaccgtgt gggaaaatat
     5341 ctgattgata agtcttttaa tcagtattct aaaaaagcca ttctaaacga gagccgctta
     5401 aagactttac atagtctttt aaatactatt tttcattata cccttggttt cttttttatc
     5461 tatgcaattt tatctgctat cggtgtgccg atcggatcac ttttggctgg tgccggaatt
     5521 gcaggtgtgg caatcggtct tggtgcccaa gggttcatga gtgatgttat taccggattt
     5581 tttattatca tggaacaaca gatggatgtt ggggactaca tcaagctagc aaacttaacg
     5641 attgaaggaa ccgtcgtatc tgttggtatc cgtacccttc agctgaaagc aacggatggt
     5701 actgtccatt tcatcccgaa tcgaaacatc accacgatca gcaatctctc tcgtgcaaat
     5761 atgcaagtac ttttagatat tcggattgtt cctgaagaag gatatgacaa aatctatgag
     5821 atcattgatc gagtaaatcg tacattagct gaaaaatacc aagaagagct ccaaactgaa
     5881 ccttctatct ttggattagt ggatcttgga aacagcaatt ttgcgattcg tacggtctgc
     5941 tacgcattga acggaaaaca atatgcctta aaagaagagt tcttgagctc ctatgtcaaa
     6001 gaattgacag cttctggttt cacgattccg aacagtccaa ttgctgtcgg aaaataattt
     6061 ggacaaacga tgcaacgaat ctcactaaaa agagcgtggg acaaaagtaa agaacacttt
     6121 tgtc
//
LOCUS       DACDYJ010000109         6025 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDYJ010000109 DACDYJ010000000
VERSION     DACDYJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.111"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            77..1825
                     /locus_tag="IWM51_002616"
                     /pseudo
     CDS             77..1825
                     /locus_tag="IWM51_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333370.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
     gene            1839..2408
                     /locus_tag="IWM51_002617"
     CDS             1839..2408
                     /locus_tag="IWM51_002617"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HAP9971965.1"
                     /translation="MKIYTKSGDKGNTSTSIIGGERIKKSDARVCAYGSVDEVNSWVG
                     KIISELDDKKFELLKKELVVLQILLFDIGTDLATPIGIKEMIVGKEEVTKIETLIDFY
                     QAKVPVIEKFILPGGHPVACDMQITRTVIRRAEREITRLIVSDEPINLHAYKIMNRLS
                     DLFFVLARYVNHVYATKEPFYERAGKVFH"
     gene            2424..2828
                     /locus_tag="IWM51_002618"
     CDS             2424..2828
                     /locus_tag="IWM51_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP9971966.1"
                     /translation="MKKLATLLTVFIGLFIMTGCQSNQNDQASSKASTQATSEVAKSS
                     ALIKLTDSDKEITSKTVTYKSGESLLVILKANFEVKERDGFITSIDGHAQDESQNKYW
                     TFTINGKMGEKGAGETTLDNDDQVVFNLGVFK"
     gene            3030..3704
                     /locus_tag="IWM51_002619"
     CDS             3030..3704
                     /locus_tag="IWM51_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9971967.1"
                     /translation="MSNFKGKQFEKDIIMVAVCYYLRYNLSYRDVSEILNERGINVCH
                     TTVYRWVQEYSQVIYCLWKKRNKSVGDSWRMDETYIKVKGKWHYLYRSIDSSGITLDI
                     WLRKKRDKQAAYAFFKRLIKQFGEPKVLVTDKAPSLSSAFNQLHSEGLFSKTEHRTNK
                     YLNNIIEQDHRSIKKRHKLYQSIRTASSTIKGIETIHALYKISQRDKSLFGFSVIHEM
                     NTLMSI"
     gene            3776..4240
                     /locus_tag="IWM51_002620"
                     /pseudo
     CDS             3776..4240
                     /locus_tag="IWM51_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173988.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            4240..5130
                     /locus_tag="IWM51_002621"
     CDS             4240..5130
                     /locus_tag="IWM51_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9971968.1"
                     /translation="MEELIRVEHATKTFRSKNWRGTVSEVRAVEDVSLTIHRGETLGL
                     VGESGSGKTTLGRSILNLETLTSGEIFFEGKELTALTAEDRKAAYQQMQIIFQDPYSS
                     LNPRMTALELVMEPLYHETKDAARAKAKEILAIVGITGDDVHKLPRSFSGGQRQRIGI
                     ARAVVNRPAFVLCDEPTSALDVSIQAQIIQLLLRLQKEFSLSYLFISHDLSVIRHISD
                     RTAVMYQGKLVELAPTDRLFDNPLHPYTKKLLAAAPIADPVLARGAMEQTEIQSTITL
                     TPLYEWIEVEPDHFVRREKR"
     gene            5127..5519
                     /locus_tag="IWM51_002622"
     CDS             5127..5519
                     /locus_tag="IWM51_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HAP9971969.1"
                     /translation="MTVETLTIHQGSDKLEMTTPTGNWVLAREKGYSPVQMLVSAVAA
                     CGGYVYASVLENSKITAEIEKIEVSYDRDDNKKAQPVSAIHIQFYAKIAEADHDKATR
                     CLKLVSPNCPVIQSLDPAIEVTETVTFI"
     gene            5705..6025
                     /locus_tag="IWM51_002623"
                     /pseudo
     CDS             5705..6025
                     /locus_tag="IWM51_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT         1996 a         1060 c         1316 g         1653 t
ORIGIN      
        1 tattattggg aatcccagct taaatcatag ataccgtaag ggattttatt ctttatttaa
       61 aactttacaa cagaaccatt tagttgaaca aggcgggagt cgtcggggag cacaaatgat
      121 catgttgaat gattggcatc cagatatttt tgaattcatt atctctaaga ttcagaatcc
      181 ggccatttta caaaatttga tccacactag ccaaagtccg acagtgcgcc aattagctag
      241 tgaaaaactg acttttgaac cactgacagc aaatgaaaaa gaatggttag aatttttggt
      301 tgatcatccg acaaatgata tggaggataa atttttaaaa acggctcaac agaaattaaa
      361 agcgggtggt acgtacagtg tgaaagaccc tgatttcttg accggagcca atatctccgt
      421 ctgtttaaca aaagaattta tggaagctgt cgaaaccgac caaacttatg agttacgctt
      481 tccagcaatc gaagactatg acgctagtga aatggctgat tatgatcaac attggatgga
      541 agttggagat gtcagagaat gggctgaaac agggcgtaaa gtaaaaatac atcaaacgat
      601 caaggctcgc gaattgtggg atttgattac ttattgtgca acgtattcag cagaaccggg
      661 aatatttttt atcgataatg ctaatgagat gaccaatgct caagcctacg gacaaaaggt
      721 agttgcaacc aatccatgtg gggaacagcc attgacgcct tatgcggtat gcaatctagc
      781 agcaattaat ttagccaata tggttgataa aaaaacgaaa gttgtcgact ttgaaaaatt
      841 aactgatgtc gtaagagcta gtgttcgttt tcaggacaat gtaattgatg cgacgcctta
      901 cttttttaaa aaaaatgagc agcaagctaa aggtgaacga cgtgttggac ttggggtcat
      961 gggattggca gatttattga tttattgtga aaaagtctat ggatctgaag atggaaaccg
     1021 tttagtagat caagtatttg aaaaaattgc tgttacggct tatcaaacat ctgttgaact
     1081 ggcaaaagaa aaaggcagct ttccattttt agttggtaaa acagctgagg aaacaaccgc
     1141 gcttcgtcaa cgttttattg agtcgggcta tatgaagaaa atgccggaat cgatccgaca
     1201 agcgattcta aaatacggca ttcgtaattc tcatttacta acagtcgcgc caacgggatc
     1261 aacgggaact atggccggtg tttcaactgg actagaacca tatttctctt tttcttattt
     1321 tagaagtggt cgtttaggaa aattcattga agttaacgct gaaatagtca gtgaatattt
     1381 agaaaaaaat ccagagaaaa ctgcggataa tctaccacca ttctttgtct ctgccatgga
     1441 gctttcacca aaagctcacg tgagtgttca atgtacgatc caacgttggg tagatagctc
     1501 tatctcaaaa acagtcaatg cacctaaggg ttatcaagtg aaacaagtag ctgcgatcta
     1561 tgaagatctg tatcaaggcg gtgctaaagg tggaactgtc tatgtggatg gtagcaggga
     1621 ttcgcaagtg ttgacacttg aagcagatga caatgtattt gaagaacctg tagtaggggt
     1681 ggcagataat caagttactc ttgactcatt agaatcatca gaagcagaag aaatatgtcc
     1741 gatttgccat gagggtagaa ttgaagaaat tggtggttgc agtacctgca ctaactgtaa
     1801 agtacaacta aaatgtggct tgtaaaagga gctattcgat gaaaatttat actaaaagcg
     1861 gcgataaagg aaatactagt actagtatta ttggtggaga aagaataaaa aaatctgacg
     1921 ctcgcgtttg tgcttacggt agcgttgatg aagtaaattc ttgggtcggt aaaattattt
     1981 cagagttgga tgataaaaag tttgagctgt tgaaaaaaga actagttgta ttacaaattt
     2041 tactttttga tattggaaca gacttggcta cacccatcgg gattaaagaa atgattgtag
     2101 ggaaagaaga agtaactaag attgaaacgc tgattgattt ttatcaagca aaagttccag
     2161 tcattgaaaa atttatctta cctggaggac atccggtggc atgcgatatg caaattacac
     2221 gaacagttat tcgtcgggca gaacgtgaaa tcacacgatt gattgtttca gatgaaccta
     2281 tcaatttaca tgcatacaaa attatgaatc gcttatcaga tttatttttt gtacttgcac
     2341 gttatgtaaa tcatgtttat gctaccaaag agccatttta tgaacgagca gggaaggtat
     2401 tccattaaga gagggtttta aacttgaaaa aattagcaac attattaacc gtttttatcg
     2461 gactattcat tatgacaggg tgccaatcaa atcaaaatga tcaagcctct tcaaaagcat
     2521 ccactcaagc aacaagcgag gttgctaaat caagcgcgct gataaaattg actgatagtg
     2581 ataaagagat cacttcaaaa acagtcactt acaaatcagg agaatcatta ttggtaattt
     2641 taaaggctaa ctttgaagta aaagaaaggg atggctttat tacttcaatt gatggtcatg
     2701 cgcaagatga gagtcaaaat aaatactgga cattcactat caatggaaaa atgggtgaaa
     2761 aaggcgcagg tgaaactaca ttagataatg atgatcaggt agttttcaat ttgggtgtat
     2821 ttaaataaaa attatcgttc tttacagcaa ttgagcgata attaagtaag agaaaattat
     2881 tagtttttaa aggaatatgt taattttaaa acacgttcaa accaatcatt tcgaaggggt
     2941 tgaacgtgtt ttttggttct gttgcaaagt tttatctaaa tacctattta gtgtaaaata
     3001 gctgaaacta aaacataagg agaattacca tgtccaattt taaaggtaaa caatttgaaa
     3061 aggatattat catggtagcc gtatgctact atttaagata taacttaagt tatcgtgatg
     3121 tttcagaaat ccttaacgaa cgtggaatca atgtttgtca cacgacggtt tatcgatggg
     3181 tacaagaata tagtcaagtt atttattgct tatggaagaa aagaaataaa tctgttggcg
     3241 attcctggag aatggatgaa acttatatta aggtaaaagg aaagtggcac tatttgtatc
     3301 gatccattga ttcttcgggg ataacattag atatttggtt gcgaaaaaag cgtgataaac
     3361 aagcagctta tgctttcttt aaacgattga tcaaacaatt tggagaacct aaagttcttg
     3421 tcactgataa agccccttca ctaagtagtg cgtttaatca attgcattct gaaggattat
     3481 tttctaagac tgaacaccga acaaataaat acttgaataa tatcattgaa caggatcatc
     3541 gatccatcaa gaaaaggcac aaattatatc agtccataag gactgcttcc tcaacaatta
     3601 aaggaattga aaccatacac gctttatata aaattagcca aagagacaaa agtctctttg
     3661 gcttttcagt gattcatgag atgaatacat taatgagtat ctaagcataa aatcaaaaat
     3721 taattggaaa ctctttatta attttttaac tttgcaacag aacccttttc cagtacaacg
     3781 tatgaaacaa tatccccacg agttatcagg agggatgcgt cagcgggtga tgatcgccat
     3841 ggctttattg gcgcaaccga aattgctgat cgcagatgaa ccgacgacgg ctttagatgt
     3901 gacgatccaa gcacagattt tggccttgat caaaaacctg caaagagaga accagctttc
     3961 cgtcgtcctt gtgacccatg actttggggt cgttgcagga atgtgcgact ttattaaagt
     4021 gatgtatcaa ggtcgagtcg tggaagaagg caccacggaa gagatctttt atcaacctgc
     4081 gcatccttat acgaaacaat tattgcaagc ggcacattta ggcgacaagc atgccgaatt
     4141 aaaagtcttt gactatcatg agacattggc aaatgagttg caagaaacgc agttatctaa
     4201 gacccatcgc gtttggtgca atcaggaggt gacagaataa tggaagaact gattcgcgta
     4261 gaacatgcga cgaaaacctt tcgcagcaaa aactggcgcg gcactgtcag tgaagtccga
     4321 gcggtcgaag atgtcagtct aacgatccat cggggcgaga cattggggct agtaggggaa
     4381 tctgggagtg ggaaaaccac tttaggacgc agtattttaa atttagagac tttaacaagc
     4441 ggggagattt tctttgaagg caaagagcta acagcattga cggcagaaga tcgcaaagca
     4501 gcgtatcagc aaatgcagat cattttccaa gatccgtatt catcattgaa tccccgcatg
     4561 acggcacttg aactggtgat ggagccgttg tatcatgaaa cgaaagatgc tgcccgagcg
     4621 aaagccaaag aaatcttagc gatcgtcggg atcacgggtg atgatgtcca taaactgccc
     4681 cgcagtttca gcggcggaca acggcaacgg atcggcatcg ctcgtgcggt ggtcaatcgc
     4741 ccggcgttcg tcttgtgtga tgaaccgact tcggcattag atgtctccat tcaggctcag
     4801 atcatccaat tgctgctgcg tttgcaaaaa gagttttcat tgtcttattt gttcatttcc
     4861 catgacctgt ccgtgattcg tcatatcagt gaccgaacag ccgtcatgta tcaagggaaa
     4921 ctagttgaat tggcacccac cgaccgtttg tttgacaatc cgctgcatcc ctacacgaag
     4981 aaattattag cagcagcgcc gatcgctgat cctgtgttag caagaggtgc gatggaacaa
     5041 acggagattc aaagcacgat tactttgaca ccattgtatg aatggatcga agttgaacca
     5101 gatcattttg tgaggagaga aaaacgatga cagtagaaac attgacgatc catcaaggaa
     5161 gcgacaaact tgaaatgacg accccaactg gcaattgggt gttagcacgg gaaaaaggct
     5221 attcgccagt ccaaatgctg gtgagtgcgg tcgctgcttg cggtggctat gtatacgcat
     5281 ccgtcttaga gaattcaaaa atcacagcag aaatcgaaaa gatcgaagtc agctatgacc
     5341 gagacgacaa caaaaaagcc caaccagtca gtgccatcca cattcagttt tatgcgaaaa
     5401 tcgctgaagc cgaccatgac aaagcgaccc gttgcctaaa actcgtttca ccaaattgcc
     5461 ccgtgatcca atcattagat ccagcgatcg aagtgacgga aacggtgacg tttatttaat
     5521 cgaaaaagac tttgtgtgcc tgaaatcaat aggcgtacaa agtcttttgg ctctataatt
     5581 tatgggactg atgaatcaga attgattctt cagccccatt ttcattttag gtattaaaaa
     5641 acatatcgtt ctctagtatg attaaatcac cacaaaataa acaactgaga ggacgatatg
     5701 ctatatgaat gattctatca aaaaaatgct gagaataata gagaaagatt tgatgattac
     5761 agaggtctct tacgagaccc ttcagaagaa aaagacgttg gtcgtcgatg ctgttctctc
     5821 gcctactcct cgtgcttgta gaagttgtgg ttctactgtg gtagatggaa acgggaaagc
     5881 aattatagtg aaaaatggga agaaggaaac gattgtccgt tttgaacaat aaaatcatat
     5941 gcctttggtt atgcgcctaa aaaagcagcg ctatacctgt aaaaactgtc gcacccattg
     6001 gactgctcaa agttattttg tccaa
//
LOCUS       DACDYJ010000110         6023 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDYJ010000110 DACDYJ010000000
VERSION     DACDYJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.112"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            80..778
                     /locus_tag="IWM51_002624"
     CDS             80..778
                     /locus_tag="IWM51_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9971970.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(946..3585)
                     /locus_tag="IWM51_002625"
     CDS             complement(946..3585)
                     /locus_tag="IWM51_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9971971.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(4204..5235)
                     /gene="queA"
                     /locus_tag="IWM51_002626"
     CDS             complement(4204..5235)
                     /gene="queA"
                     /locus_tag="IWM51_002626"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP9971972.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            5428..>6023
                     /locus_tag="IWM51_002627"
     CDS             5428..>6023
                     /locus_tag="IWM51_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9971973.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         1731 a         1301 c         1143 g         1848 t
ORIGIN      
        1 aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg gtgttattaa
       61 agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag caaaagagag
      121 tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat tgattggtat
      181 tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca tatatgatgg
      241 atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag gcggtggcgg
      301 aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga cttatcttga
      361 tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc gtggattcaa
      421 aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct ttacaacatt
      481 atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt ctcaaagcta
      541 ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac tggatacgat
      601 cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt tagatctttc
      661 atttatcggc tggcatatct tagcattagc aactgctggt attggttatc tatggctcag
      721 cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag atatttaaaa
      781 gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga gaggcagtaa
      841 aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc acacctcctt
      901 tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt cctcttcttc
      961 tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta cgatagataa
     1021 aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata gttcttgcgt
     1081 aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca tttctaccaa
     1141 agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta cattgaacac
     1201 atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga catgctgact
     1261 tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac caaacaaggt
     1321 tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc gagcaaagat
     1381 cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg gctctcttcc
     1441 taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt gcacagctgt
     1501 aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct ccgaaatatt
     1561 acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac gtccttgact
     1621 gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg cggcactttg
     1681 cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt tcaacgctgg
     1741 tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt gccaagattt
     1801 gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag ctttgacacg
     1861 cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg cttctgacgg
     1921 atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat gatctccagt
     1981 gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt tactttctgg
     2041 acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt ccagtgcttc
     2101 agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa tcactctcag
     2161 tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc ctgaactaaa
     2221 gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt cgcccatttg
     2281 gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta ctcgtggata
     2341 tttttcttct aattctgcct ttgtatgata attctcttca acagccctta cgatggctac
     2401 ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg tcacatctgt
     2461 acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat cttcgatatt
     2521 cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt gtgtcagcgt
     2581 tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg ctggcaactg
     2641 tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg tcaacgtaac
     2701 aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag tcatgaacat
     2761 ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg cagccgctaa
     2821 agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag gtgttttttg
     2881 catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca tgccaatggc
     2941 agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata ccatattaag
     3001 ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt caactgcttc
     3061 actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga tcaaacgggc
     3121 atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta ccagttgctc
     3181 tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt tattcatatc
     3241 ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca ataccgagtt
     3301 gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt ctgtagcaaa
     3361 tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa aatcggacaa
     3421 gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt tattcaatcc
     3481 ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg gatctgcttc
     3541 tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat tcctccttta
     3601 aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt atttacttca
     3661 aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc ttatatttgc
     3721 aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca tcaaatagac
     3781 agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta caggtttcac
     3841 ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat gtttggatca
     3901 cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt aaacgtctga
     3961 aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa caaaattttt
     4021 gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt tacaatcatt
     4081 tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga gctcggcttt
     4141 ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg gcttttcata
     4201 accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct cttcaatcgc
     4261 atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta acatgactaa
     4321 agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga attcatagcc
     4381 aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat taaatttcgt
     4441 tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt tcccgccatt
     4501 tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga attcactatg
     4561 catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta accctacatg
     4621 tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta attctttcgt
     4681 aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat agacagtttg
     4741 atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca tttctcccaa
     4801 tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc gtccgccgtg
     4861 ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga ttcttgtccc
     4921 aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt gtgtattcgt
     4981 tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata aacgagcagg
     5041 cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt caatgatttc
     5101 atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta aacgagaatg
     5161 atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa aatcaaaatc
     5221 ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt ctatcatgtt
     5281 tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt cctaaataaa
     5341 aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag atagttcttc
     5401 attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc aaaaatttat
     5461 aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg tgagttcatt
     5521 tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat caaccgaatg
     5581 actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga aattgatcca
     5641 gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat gcctcacatg
     5701 acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca agaagaacgg
     5761 aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat gttagatcgc
     5821 tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg tgcccttgca
     5881 gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc aatcactcgt
     5941 gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac gatcgtattt
     6001 gtaacccatg acatggatga agc
//
LOCUS       DACDYJ010000111         5951 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDYJ010000111 DACDYJ010000000
VERSION     DACDYJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.113"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..450)
                     /locus_tag="IWM51_002628"
     CDS             complement(<1..450)
                     /locus_tag="IWM51_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9971974.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="IWM51_002629"
     CDS             674..1465
                     /locus_tag="IWM51_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9971975.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="IWM51_002630"
     CDS             1431..2384
                     /locus_tag="IWM51_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAP9971976.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="IWM51_002631"
     CDS             complement(2782..3756)
                     /locus_tag="IWM51_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9971977.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="IWM51_002632"
     CDS             3871..4305
                     /locus_tag="IWM51_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9971978.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5416)
                     /locus_tag="IWM51_002633"
     CDS             complement(4430..5416)
                     /locus_tag="IWM51_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAP9971979.1"
                     /translation="MEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGAFFRVHHFAPQIG
                     SPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQGRVQLGVGRGSPE
                     QVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPMFPQGSGKLRIEP
                     YSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQQANQLRAYKEAW
                     KQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYHRNPTIFGKTFAG
                     EPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIAPELGWR"
     gene            5844..>5951
                     /locus_tag="IWM51_002634"
     CDS             5844..>5951
                     /locus_tag="IWM51_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAP9971980.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSA"
BASE COUNT         1871 a         1122 c         1022 g         1936 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaaaaaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga
     5941 taagaagtgc g
//
LOCUS       DACDYJ010000112         5877 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDYJ010000112 DACDYJ010000000
VERSION     DACDYJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.114"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            641..1375
                     /locus_tag="IWM51_002635"
     CDS             641..1375
                     /locus_tag="IWM51_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9971981.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="IWM51_002636"
     CDS             1391..2560
                     /locus_tag="IWM51_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9971982.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="IWM51_002637"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="IWM51_002637"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9971983.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="IWM51_002638"
     CDS             3650..4576
                     /locus_tag="IWM51_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP9971984.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="IWM51_002639"
     CDS             4595..5845
                     /locus_tag="IWM51_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9971985.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1968 a          955 c         1203 g         1751 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacaac aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta gttaagt
//
LOCUS       DACDYJ010000113         5797 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDYJ010000113 DACDYJ010000000
VERSION     DACDYJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.115"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(192..662)
                     /locus_tag="IWM51_002640"
     CDS             complement(192..662)
                     /locus_tag="IWM51_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP9971986.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            992..2632
                     /locus_tag="IWM51_002641"
     CDS             992..2632
                     /locus_tag="IWM51_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP9971987.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2786..4108
                     /locus_tag="IWM51_002642"
     CDS             2786..4108
                     /locus_tag="IWM51_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP9971988.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4176..4610)
                     /locus_tag="IWM51_002643"
     CDS             complement(4176..4610)
                     /locus_tag="IWM51_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9971989.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4675..5511)
                     /locus_tag="IWM51_002644"
     CDS             complement(4675..5511)
                     /locus_tag="IWM51_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP9971990.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         1732 a         1157 c         1064 g         1844 t
ORIGIN      
        1 tgacttcaca gtctcttcct taaacggtgt tcaaaaactt gcatctgcgg taataagccc
       61 aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcagg
      121 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
      181 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      241 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      301 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      361 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      421 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      481 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      541 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      601 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      661 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      721 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      781 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      841 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      901 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      961 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
     1021 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
     1081 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1141 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1201 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1261 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1321 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1381 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1441 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1501 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1561 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1621 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1681 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1741 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1801 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1861 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1921 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1981 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     2041 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     2101 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2161 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2221 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2281 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2341 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2401 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2461 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2521 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2581 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2641 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2701 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2761 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2821 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2881 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2941 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     3001 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     3061 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3121 cagcaatatg ggctttttgg acttttatat tgctgccttg attactggta gcatccttgg
     3181 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3241 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3301 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3361 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3421 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3481 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3541 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3601 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3661 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3721 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3781 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3841 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3901 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3961 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     4021 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     4081 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4141 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4201 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4261 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
     4321 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
     4381 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
     4441 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
     4501 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
     4561 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
     4621 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
     4681 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
     4741 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
     4801 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
     4861 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
     4921 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
     4981 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
     5041 accaccaata cctaaaaaac tagctaaaaa gcccagaacc cccccacata taaggtacca
     5101 agtccatgac gtcaaatgaa attttggctg atgatgcttg gtgtagaaga acgcaaaaac
     5161 caaggtcaat accgtcaaaa aaatttggat cagctgcaca tgcttctcgt tttcaaataa
     5221 ctgtaaaaaa acttcgaata caatgttgcc gactactcct ccaacaacag ctccactaga
     5281 tacccaaccg acaatctgcc aattcagtga tttcccgctt gatacttgtc gcacagtcga
     5341 aacaatggac atcgtgaata ccgcaactgt tgagtaaaaa gaaatccctg caacagaatg
     5401 ggcgccgata agatcaagaa tcggtttgat caacacgcct ccacccatcc cggaaatagc
     5461 gccaactgtg ttcgccagaa cgatcaccac aaaataaatc agaccaatca tcttgtattt
     5521 cctttctctt gtttatatct tttagcgatt gttactcttt tcttttatta tacagtaaaa
     5581 gaaacaaacg aggcttcgaa agcttttcta ctttttaagg aaatttagtg ctaaacgaaa
     5641 agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagctc
     5701 ctcataattt tcgtttggtt gaacttatta ccgcagatgc aagcttttga acaccgttta
     5761 agaaagaggt cgtgagcctg ccgttcagct ccgagtc
//
LOCUS       DACDYJ010000114         5727 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDYJ010000114 DACDYJ010000000
VERSION     DACDYJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.116"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            196..921
                     /locus_tag="IWM51_002645"
     CDS             196..921
                     /locus_tag="IWM51_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971991.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="IWM51_002646"
     CDS             911..1420
                     /locus_tag="IWM51_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9971992.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="IWM51_002647"
     CDS             complement(1490..2938)
                     /locus_tag="IWM51_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9971993.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="IWM51_002648"
     CDS             complement(2938..3654)
                     /locus_tag="IWM51_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9971994.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="IWM51_002649"
     CDS             complement(3635..3985)
                     /locus_tag="IWM51_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9971995.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="IWM51_002650"
     CDS             4129..4902
                     /locus_tag="IWM51_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9971996.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="IWM51_002651"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="IWM51_002651"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1662 a         1124 c          994 g         1947 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt tttttta
//
LOCUS       DACDYJ010000115         5658 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDYJ010000115 DACDYJ010000000
VERSION     DACDYJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.117"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..663
                     /locus_tag="IWM51_002652"
     CDS             <1..663
                     /locus_tag="IWM51_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP9971997.1"
                     /translation="FGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPF
                     TNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSA
                     HKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERSNGTYDFDRFYQGKGDINAQ
                     ILFSYKAEQYYKGYKKHEHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQE
                     RE"
     gene            674..2878
                     /gene="topB"
                     /locus_tag="IWM51_002653"
     CDS             674..2878
                     /gene="topB"
                     /locus_tag="IWM51_002653"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9971998.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSLLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKSQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYHKAAACTSEACDFRLWTTIAKKKLTATQLKEII
                     QNGRTSQPVKGLKGQKGSFEATIVLKEDFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            3124..3720
                     /locus_tag="IWM51_002654"
     CDS             3124..3720
                     /locus_tag="IWM51_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9971999.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGKIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTIRSYIMALN"
     gene            3733..4632
                     /locus_tag="IWM51_002655"
     CDS             3733..4632
                     /locus_tag="IWM51_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9972000.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFENRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYEETMDSISNLMEKVI
                     HVEEKERKIAYQRHQQMNREMER"
     gene            4635..5120
                     /gene="ssb"
                     /locus_tag="IWM51_002656"
     CDS             4635..5120
                     /gene="ssb"
                     /locus_tag="IWM51_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9972001.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGILIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
BASE COUNT         2063 a          997 c         1184 g         1414 t
ORIGIN      
        1 tttggtgagg cagtatctga attgagaaag aaacaagcct ttcctcaatc catcgagatt
       61 tgtgtggata atgaccgtgc aggacatatt ttctacgaaa aagaacaatt aatgggcgca
      121 gttgatccat ttacgaatca gaaagtacgt tgtgaacgag gaatcgctaa tgattggcaa
      181 gtgccaaaag aatacaaagt catttatgaa gaagtcgcaa aagaagaaaa gatcacacca
      241 gaagctatta tggccataca caagacagaa aacaatctgc aattaacgaa ccaattggtt
      301 tccgctcaca aagtaaatgc gtcttttggt cagcagcttt ctgttaacga ttcaatagaa
      361 gctattaatt tgaaagatat ttgtagagaa gtcgcaaaag aattaaaagc ttgtgaacgc
      421 tcgaatggta cgtacgattt tgatcgtttt tatcaaggaa aaggggatat taatgctcag
      481 attcttttct cttataaagc agaacaatac tacaaaggct ataaaaagca tgaacatgaa
      541 tttgtacctg aagtgaaaaa ggattggaac gatcaattga agcatgagat ccagcaacaa
      601 gaaattagaa agcaaaaacg tgcgatgttg tttcaacaag gcagacaaca agaaagggag
      661 tgagatcgtc acgatgaaaa ctgtcatttt agcagaaaaa ccgtcacaag cgaaggctta
      721 tgccgatagc ttttctaaag ccacccgaaa agacgggtat tttgagatcc aagatcgatt
      781 gtttcctggt gaaactgtga ttacgtatgg ttttggtcat ttagtcgagt tagattcgcc
      841 cgatatgtac gatgaaaatt ggaaacaatg gtcattagaa catttaccta ttttccctac
      901 acattatcac tatcatgttc ctaaagataa gaaaaagcaa ttcaacgtgg tgaaacagca
      961 acttcaatcg gccgacacca ttattattgc gacagatagc gatcgcgaag gggaactgat
     1021 cgcttggacg atcattcaac aagcaggcgc cgatcatgga aagatattta aaagactttg
     1081 gatcaactcc ttagaaaaag aagccattta tcagggattc cagcagttac gagatgcaga
     1141 agagacctac ccaaaatttg aagaagcaca agcacgccag atcgccgatt ggttgatcgg
     1201 catgaacggt agcctactgt atagtttact cttgcagcaa aaaggcatac caggaagttt
     1261 ttccttaggc agagtccaaa ccccaacgct ttacatgatt tatcaattac aggaaaagat
     1321 tagaaacttc caaaaagaac catattttga aggaaaatcc caagtaatcg ctcaaaatgg
     1381 cgcatttgat gcgaagcttg atccgaatga aacccaagcg acgcaagaag catttgaaga
     1441 ttatttaaaa gaaaaggggg ttcaattggg aaaacagcca ggtacgatcc accaagttga
     1501 aaccgagaaa aaaagtgcgg caagtccacg tcttttttct ctatccagcc ttcaatctaa
     1561 aatgaatcag ctcatgaaag ctagtgccaa agatactttg gaagccatgc aagggttgta
     1621 tgaaggaaaa tatctaagct atccgcgaac ggatacccct tatattacgg aaggggaata
     1681 tgcgtatctg ttggatcact tagacgaata caagcatttt ttaaaagccg aagcgattcc
     1741 gactcctatt catacaccga atagtcggta tgtgaataat aagaaagtcc aggaacatta
     1801 cgcgatcatt cctacaaaaa cggttatgac agcagcggct tttgaacaac taagtccact
     1861 gcagcaggct atttatgaac aagtgttaaa aacaacggtg gccatgtttg cggaaaagta
     1921 tacctatgaa gaaacaacga tcttgacgca agttcagcag cttcagctaa agacagtcgg
     1981 aaaagtacca gtagatctag gttggaaaaa gctgtttggc aaagagagcg acagaaaaga
     2041 aaaggaagaa gaaccgctac ttcctaaagt gacaaaagga gaagcggtga cggtagatct
     2101 acaagtcctt gaaaaagaaa cgaagccacc acaaccttat accgaaggta cgttgatcac
     2161 agcgatgaaa acagctggaa aaaccgtgga tagcgaagaa gcacaatcca tattgaaaga
     2221 agtagaagga atcggtacag aagcaacacg agcaaatatt attgaaactt tgaaacaaaa
     2281 ggaatatatc aaggtagaaa aaaataagct tgtggtgaca aataaaggga ttttactttg
     2341 ccaagccgtg gaaaaagagc cgctgctaac aagtgccgaa atgaccgcca aatgggaaag
     2401 ttacttatta aagattggtg agcagaaagg aacacaagct actttcttag ctaatatcca
     2461 aaaatttgtc tctcacttgt tggaagtcgt accaggacaa atccaatcca ctgattttgg
     2521 atcgacgtta caagaggtga aagcagccag tgaaaagcaa gaagccgcgc gtcatttagg
     2581 gatttgtcca aaatgccagg aacaagaagt tttactctat cataaggcgg ccgcatgtac
     2641 gtctgaagct tgtgatttta gattatggac aacgattgcc aaaaagaaac tcacagcgac
     2701 acagctgaag gaaatcattc aaaatggccg aacaagccag ccagtgaaag gattaaaagg
     2761 tcaaaagggt tcgtttgaag caacgattgt tctaaaagaa gacttcacaa cgagctttga
     2821 attttctgaa aagaaaaaaa cgaactataa gaaaagaaca cgccgcacta caaaataatg
     2881 gaggggttat cttatgcgaa atacaacaca caaacaacaa gaaatcgaaa atcaaaaagc
     2941 aaaaattcgt tcagaacaag agaaattacg tgccagtaaa agaagacttc agcaattaaa
     3001 gaaccagcaa cggattcaaa aagaaattga attagatcga taaaatcttc ctggctattg
     3061 tatacgcata aacttatgtt atactatatt tataagttta tacgtataca aaggagggag
     3121 aaaatgacaa aagcaatggt ttacaagctg ctagaagaat ttaacggaac tttggctttg
     3181 aaggatgcca aaaaagcggg catttctcct gtaacgatca aacgtatggt agatcgtgga
     3241 gagctggatc gtgagtatcc aggttttttc accttacctg gtcaatttcc tgatgaattg
     3301 tttatggcac aaaagaaata tgagcgcggc attatttcgc atatcaccgc gttggattta
     3361 tatgatttaa cggatatgat ccctagacag atcgatctaa ccgtacctta cggttatcac
     3421 gtttctgaaa aggggttgaa agaattcgca gttgagttac actattcaaa agcggaatgg
     3481 tatgaattag gtaagatcga aataaagagt cgttatggga atcctgttat agcttatgat
     3541 ccagaaagaa ccttgtgtga catttggaat ccctggtaca acgttgagga tgaaatcaaa
     3601 gtaaaagcaa tcaaaaacta catggagtcg gacagaaaaa atcttagaaa attgaatgag
     3661 tatcgacgaa ttttgcctac agacaaaacg atacgctctt atattatggc tttaaattaa
     3721 aggagaagac acatgacacc tacgcaatta aaagcaaaag tgaagaatat ttccagagaa
     3781 aaagaagttg atccgcagct tgttttaaga cattttatga tggaaaaatt cttggaaaaa
     3841 atctcagagt ctccttatcg tgaaaatttt gtgttaaaag gcggcttctt aattggctca
     3901 aagtatggca ttgaaaatcg aacaacaaaa gacattgata cgactttacg ggaaatgaaa
     3961 gtaaccaaag aaacattgac aacggttcta aacgatattt tttcaacgcc gacgaaagaa
     4021 ggcattcaat ttgagattca aggaatgaaa gaaacaaggg aagctgatta ttatccaggg
     4081 tttagcttac gtgtactggc acatttggaa aacatgcggc cagattttaa ggtagacgtg
     4141 acgacaggtg actcgatcta tcctgcaacg atcactcaca gtcataaatt gatgtttgaa
     4201 aatcgaacga ttcctttgga atcttatcct acagaacaaa ttattgccga aaaattaagt
     4261 gcaacctttg attttttaac agataattcg agaggaaaag atttttatga tctctatact
     4321 attcctaaaa tggaaaagat cgatgagaag accttgaaag atagtgtgag aaatacattt
     4381 acacgtcgag gaaaaactga tccgttaaag atttattacg aaaaggatat ggatgtactg
     4441 aaaaatagtc atgagctaaa agatacctgg aagaaatatc aaaaaagcaa tccttatgct
     4501 gcgtcgatca cgtacgaaga gacaatggat tcgattagta atttgatgga aaaagtgatt
     4561 cacgtcgaag aaaaagagcg aaaaatagct tatcaaagac accaacagat gaacagagaa
     4621 atggagagat aaaaatgatc aataatgtaa ctttagtagg acggttaacc aaagatccag
     4681 atttacgtta taccgcaagt ggcacagcag tggctacttt taccctagcc gttaatcgta
     4741 atttcacgaa tcaaaacggc aatcgagaag ctgattttat caattgcgtg atttggcgca
     4801 aaccagcaga aacgatggcg acattagcta aaaaaggcat cttgatcggc gttgtcggac
     4861 ggattcaaac acggacttat gacaaccagc aaggacaacg tgtgtatgtg acagaagttg
     4921 tagctgataa cttccaacta ttagaaagca aagcagcgac agagagccgt gcgcacgctg
     4981 atcaatcgtc cacgagtcca agcaccacca cttttgaaca acgggataca gcgacgccta
     5041 acaacaatgg attgaacgct tcacagaatc catttggtgg acaatcaatc gatattagcg
     5101 atgatgatct accgttttaa gtagcatttt ctattggttg catttgctac ggtttatgtt
     5161 ataatttagt caacaaaaag aattttcaat ctataaccaa aaaccactag ctggcgggct
     5221 agtggttttc ttttacgttt ctattataag aattaacgag taggcttact gtctttcttg
     5281 gcaaatttac aatcaagcca tttgcagata tagtgcgcgg ctacttctgc catgattgca
     5341 aaaacaaaat caaaaagaat tttcaaacct ataacctcct ccccttgcca gattaggccg
     5401 gggtagcaga cagcaagact attatagcat aattgaaata tccaaatgat tggatttttg
     5461 ctataatttt agcgaggtga ttgtgatgaa caaatatgtt ggtttgcttg ataaaatccg
     5521 agtaataaaa acgcaaccgt tattggttcg ctttacttta caaacgattc atgaatcgat
     5581 taattgtgta gttgcagata ttgaaattat cgataaattg ctaattatgg atgatggaaa
     5641 atacaatatt gccgtcac
//
LOCUS       DACDYJ010000116         5652 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDYJ010000116 DACDYJ010000000
VERSION     DACDYJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.118"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            35..1057
                     /locus_tag="IWM51_002657"
     CDS             35..1057
                     /locus_tag="IWM51_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9972002.1"
                     /translation="MIAELKPKHERQNFLVRDDRLDHAVAFLWKDPQTKETVGASYQG
                     TFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLN
                     DTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGA
                     VDPFTNQKVRCERGIANDWQVPREYKVIYEEVAKEEKVTPEAIMAIHKTENNLQLTNQ
                     LVSAHKVNASFGQQLSVNDSIEVISLKDICREVAKELKDCERSNGTYDFDRFYQGKGD
                     INSQILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIHQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1068..3272
                     /gene="topB"
                     /locus_tag="IWM51_002658"
     CDS             1068..3272
                     /gene="topB"
                     /locus_tag="IWM51_002658"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9972003.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            3518..4111
                     /locus_tag="IWM51_002659"
     CDS             3518..4111
                     /locus_tag="IWM51_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9972004.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            4127..5026
                     /locus_tag="IWM51_002660"
     CDS             4127..5026
                     /locus_tag="IWM51_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9972005.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            5029..5514
                     /gene="ssb"
                     /locus_tag="IWM51_002661"
     CDS             5029..5514
                     /gene="ssb"
                     /locus_tag="IWM51_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9972006.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
BASE COUNT         2071 a          970 c         1177 g         1434 t
ORIGIN      
        1 cgattggttg attgattatg cagaaaaaga aggattgatt gccgaactaa agccaaaaca
       61 tgaacggcaa aatttcttag ttcgagacga tcgtttggat catgcggtcg cctttttatg
      121 gaaagatcca cagacaaaag agacagtagg ggcgagttat caaggaacgt tcattgatta
      181 tgaacgtttt ggtgagagag gcacatataa gcatatagat aaaaattcga cagcgaatca
      241 tggctttaac ctaaagattg gtgatcctaa acagttaaaa ttttttgaga gtagtattga
      301 tttacttagc tacgctgcac tgaatcgtga tcagttaaac gatacgtggt tagtctctat
      361 ggaagggtta aaacaccatg tgatcagtca ttattttggt gaggcagtat ctgaattgag
      421 aaagaaacaa gcctttcctc aatccatcga gatttgtgtg gataatgacc gtgcaggaca
      481 tattttctac gaaaaagaac aattaatggg cgcagttgat ccatttacga atcagaaagt
      541 acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca agagaataca aagtcattta
      601 tgaagaagtc gcaaaagaag aaaaggtcac accagaagct attatggcca tacacaagac
      661 agaaaacaat ctgcaattaa cgaaccaact ggtttccgct cacaaagtca atgcgtcttt
      721 tggtcagcag ctttctgtta acgattcaat agaagtcatt agtttgaaag atatttgtag
      781 agaagtcgca aaagaattaa aagattgtga acgctcgaat ggtacgtatg attttgatcg
      841 tttttatcaa ggaaaagggg atattaattc tcagattctt ttctcttata aagcagaaca
      901 atactacaaa ggctataaaa atcatgaaca tgaatttgta cctgaagtga aaaaggattg
      961 gaacgatcaa ttgaagcatg agatccatca acaggaaatt agaaagcaaa aacgtgcgat
     1021 gttgtttcaa caaggcagac aacaagaaag ggagtgagat cgtcacgatg aaaactgtca
     1081 ttttagcaga aaaaccgtca caagcgaagg cctatgccga tagcttttct aaagccaccc
     1141 gaaaagacgg gtattttgaa atccaagatc gattgttttc gggtgaaact gtgatcacgt
     1201 atggttttgg tcatttagtc gagttagatt cgcccgatat gtacgatgaa aattggaaac
     1261 aatggtcatt agagcatttg cctattttcc ctacacatta tcactatcat gttcctaaag
     1321 ataagaaaaa gcaattcaac gtggtgaaac agcaacttca atcggccgac accattatta
     1381 ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg gacgatcatt caacaagcag
     1441 gcgctgatca aggaaaaacc tttaaaagac tttggatcaa ctccttagaa aaagaagcca
     1501 tttatcaggg attccagcaa ttacgagacg cagaagagac ctatcccaaa tttgaagaag
     1561 cacaagcacg ccagatcgct gattggttga tcggcatgaa cggcagccca ctgtatagtt
     1621 tactcttgca gcaaaagggc ataccaggaa gtttttcctt aggcagagtc caaaccccaa
     1681 cgctttacat gatttatcag ctgcaggaaa agattagaaa cttccaaaaa gagccttatt
     1741 ttgaagggaa agcccaagta accgctcaaa atggcatatt tgatgcgaag cttgatccca
     1801 atgaaaccca agcgacgcaa gaagcatttg aagcctattt aaaagaaaaa ggtgttcaag
     1861 taggaaagca gccaggcacg attcttgaag tcgaaaccga gaaaaaaagc gcggcaagtc
     1921 cacgtctttt ttctctatcc agccttcagt ctaaaatgaa tcagctcatg aaagctagtg
     1981 ccaaagatac tttggaagcc atgcaagggt tgtatgaagg gaaatatcta agctatccac
     2041 gaacagacac cccttatatt acagaaggag agcatgcgta tctgttggat catttagatg
     2101 aatacaagca ttttttaaag gctgaatcat tccctactcc tattcataca ccgaatagtc
     2161 ggtatgtgaa taataagaaa gtccaggaac attacgcgat cattcctaca aaaacagtta
     2221 tgacagaagc aacgtatgaa aagctgacac ctttacaaca agcgatttat gatcaagttt
     2281 taaagacgac ggtggccatg tttgcggaaa aatataccta tgaagaaact acgattttga
     2341 cacaagtcca gcagcttcag ctaaaggcag tcggaaaagt accagtagat ctaggttgga
     2401 aaaagctatt tggcaaagag agcgacagaa aagagaagga agaagaaccg ctacttccta
     2461 aagtaacaaa aggagaaacg gtgacggtag atctacaaat ctttgaaaaa gaaaccaagc
     2521 caccacaacc ttacacggaa ggtacactaa tcaccgccat gaagacagct ggtaaaaccg
     2581 tggatagtga agaagcacaa tccatattga aagaagtgga gggaattggt acagaagcga
     2641 cacgagcaaa tattattgaa accttgaagc aaaaggaata tatcaaggta gagaaaaata
     2701 aacttgtggt gacgaataaa ggaattttgc tttgtcaggc tgttgaaaaa gaaccgctgt
     2761 taacaagtgc cgaaatgacc gccaaatggg aaagctatct tacaaagatt ggtgagcaaa
     2821 aagggacaca agctactttt ctagcaaata tccaaaaatt tgtttcccac ttgttggaag
     2881 tcgtcccagg acaaatccaa gccactgatt ttggagcaac gttacaagag gtgaaagtag
     2941 ccagtgaaaa gcaagaagcg gcgcgtcatt taggatcttg tccaaagtgc caggaaaaag
     3001 aagtcatact ttatcagaag gtggcgactt gcacgtctga agcctgtgat tttaaattgt
     3061 gggtaacgat tgccaaaaag aaactcacag cgacacaact gaaagagatc attcagaacg
     3121 gccgaacaag tcaaccagtc agaggactaa aaggtcagaa aggttcgttt gaagcaacga
     3181 tcgttttaaa agaagacttt acaacaggat ttgagttttc tgaaaagaag aagaccacct
     3241 acaaaagaaa agtgagacgt aaaacgagtt agaattgacg gagggaaaaa cgatgagaaa
     3301 tacaacgcat aaagaacaag aaatcgaaaa tcaaaaagcc aaaatacgtt cagagcagga
     3361 gaaaatgcgt gccagcaaac gaagactaca gcaattgaag aatcaacaac ggattcaaaa
     3421 agaacttgaa ttagatcgat aaatcctcct gttgttaata ttaaactaac atgttatagt
     3481 tatgttagtt taatattaac attcgagagg gaaagcgatg aaagataaag tcaagtgcat
     3541 ttttgagaaa tacaatggta atctatcctt gaaggatgct agaaatgaag ggttatcacc
     3601 tatgacgtta gatcgtttag ccgaaaagga caaaatagaa aaaatagcat caggttttta
     3661 taccttaaaa gatagttttg tggatgaatt atatctcgct caaagtatct atactagagg
     3721 aatcttctct cacgagacag cgcttgatct ttttcaagta ggaacatata tccctaaaaa
     3781 aattaatatg atgtttccta aaggatacaa tgtttcgaaa gagaatttag atcagtatgc
     3841 cattcaaccg cattacacga ataaagaaaa ctttgaactg ggaatcacag aggcagaaag
     3901 cttttacggc aataaaattt tcgtctatga tttagagcga acgttatgtg atatgtggaa
     3961 tcccagatat aaagcaagcg tagaagtaaa acaagaagca ttaaaagaat atatgacaag
     4021 taccagcaga aatacaaata agctaagaag atacatgaat attttaaaat caccaaaaga
     4081 aatgactttg tatatgttgc cactttattg agaggagtaa aggtgaatga caccgacaca
     4141 atttaaagcg aaaacaagaa atgttgcagc tgagaaacat ttaaacccac aaattgttca
     4201 aagatattat atgatggaaa aattattaga atgtattgtt gaatcgcctt atcatgatga
     4261 ttttgtatta aaagggggat ttctgatcgg atcaaaatac gggttagaaa accgttcaac
     4321 cgttgatatt gatacgactt atcgaaattc gaaactaaca aaagaaaaat tagaaactgt
     4381 tttttctgaa ttaacagctg ctcctacaaa agatggcatt cggtttgaat taaagggctt
     4441 aacagagacg agggaagccg attattaccc aggttttcaa gcaaagttta ttgcttttct
     4501 tgaaaatgca cgtgtacctt ttaaattaga tattacttca ggtgattcta ttattccagc
     4561 agcattgata tatgaacata aattgatgtt tgaagataaa acaattcata ttccagctta
     4621 tcctacggaa caaattttag ctgaaaaatt acatgccacg tttagtttcg gaatagataa
     4681 ttcacgtatg aaagatttct atgatctgta tatgatccct aaactagaac gtatcgatga
     4741 gataaatttg tataagagtg ttcaaggaac atttaaggaa agaaataatc aatcagtgtt
     4801 taagaatttg tataatcagg aaatgcctca attgatgaag aatgagggat tgagggagaa
     4861 atggaaaaga tatcagcaag aaaactattt tgccaaagat atttctttcg aatctacctt
     4921 aacttctgtt gattcgctga tgcaatcaat tgtcgagcaa gaggaagtag aacgaaaaca
     4981 aaattttaag agatcacaac aaatgaatca agaaatggag agataaagat gatcaataat
     5041 gtaactttag taggtcgatt aaccaaagat cccgatttac gttacaccgc aagtggcact
     5101 gcagtgacca cttttacctt agccgttaac cgtaatttta ctaatcaaag tggcaatcga
     5161 gaagctgact ttatcaattg tgtgatttgg cgcaaaccag ctgaaacgat agcgacatta
     5221 gctaaaaaag gcagcttgat cggcgtcgtc ggacggattc aaacgcggac ttatgacaac
     5281 cagcaaggac agcgtgtcta tgtgactgaa gttgtggccg ataatttcca actattagaa
     5341 agtaaagcag cgacagaaag ccgtatgcac gctgatcaat cgtccagcag tccaagccct
     5401 accacctttg aacaacggga tacagcgacg cctaacaaca atggattgaa cgcttcacag
     5461 aatccatttg gtggacaatc aatcgatatt agcgatgatg atctaccgtt ctaagtagca
     5521 ttttctattg gttgcatttg ctacggttta tgttataatt tagacaacaa aaagaatttt
     5581 caatctataa ccaaaaacca ctagctggcg ggctagtggc tttcttttac gtttctatta
     5641 taagaattaa cg
//
LOCUS       DACDYJ010000117         5606 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDYJ010000117 DACDYJ010000000
VERSION     DACDYJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.119"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            291..1265
                     /locus_tag="IWM51_002662"
     CDS             291..1265
                     /locus_tag="IWM51_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9972007.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1247..1708
                     /locus_tag="IWM51_002663"
     CDS             1247..1708
                     /locus_tag="IWM51_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9972008.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1758..2222
                     /locus_tag="IWM51_002664"
     CDS             1758..2222
                     /locus_tag="IWM51_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9972009.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2236..2985
                     /locus_tag="IWM51_002665"
     CDS             2236..2985
                     /locus_tag="IWM51_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9972010.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2985..3815
                     /locus_tag="IWM51_002666"
     CDS             2985..3815
                     /locus_tag="IWM51_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9972011.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3815..4462
                     /locus_tag="IWM51_002667"
     CDS             3815..4462
                     /locus_tag="IWM51_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9972012.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKINYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4560..5336)
                     /locus_tag="IWM51_002668"
     CDS             complement(4560..5336)
                     /locus_tag="IWM51_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9972013.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5418..5561
                     /locus_tag="IWM51_002669"
                     /pseudo
     CDS             5418..5561
                     /locus_tag="IWM51_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1925 a          769 c          991 g         1921 t
ORIGIN      
        1 aactagatca tcaatcgtaa gcatcgcaaa ttgatttcta ccattcgtat tgtttttaga
       61 catcatagct atcactcctt gcattcgttt tcataaatct attataaaag aaaagactgt
      121 agacatcatc cataaaatgg actttgtcta cagtctggca ttttattata taaaattgtt
      181 ataaaaaatt tttttgaaaa atagtattgc aaaataaagc aatttcatag ataataggta
      241 tcgtaaagtt atataacttt ataataccta ttagagtgag ggataaagaa atgagacaac
      301 tacacaaaga acagattgaa acagcaatgg cagcgttaaa ggaaaagaca attgatcatg
      361 tatattttac agcatgtggg ggttcacaag ccgttttaat ggctggtcaa tatctatttg
      421 ataaagaatt aactgtacct tgtcatgttt atactgctaa cgaatttaac tatgctatac
      481 caaaaggatt atctgaaaat tctttagtta tcacttgttc ccattctggc acaacaccag
      541 aaactgtaga agcaacaaaa aaagcaagag aatttggagc aatgactatt tgtttatcta
      601 atgaagaagg atctcctcta tgggaagcag ctgaatatcc tgttcactat gattggggta
      661 aagatgttga tgctagtgat ttaaataaag gaattcttta tggtttatta tttaatattt
      721 tacttgtttt agatggctct aaaaaatggg aaacttgctt gaatgaattt gataagttaa
      781 atgatcttgt tattgctgaa aagagcaaat ttgaaataac tgctaaagaa tggggtaaaa
      841 accaaaaacg agataaaatt atttacacaa ttggtagcgg tattaattac ggagaaattt
      901 actccacagc agcttgctgg ttcatggaaa tgcaatggat taattctaat gctattcatt
      961 ctggggaatt tttccatggt ccttttgaaa tcactgatta tgatgtacca ttcatcttag
     1021 tgaaaagtat tggagataca agatttttag atcaacgggt agaagacttc actaaaaaat
     1081 tcagtaataa attaatggtt ttagaccaag ctgattttga cttagaatct atagatagtg
     1141 tagcgaaaga atatattgca gctgtactta gtggcgttgt tattcgccaa atggtagaag
     1201 cgattgcttt tgaaagaggc cattccttag atgttagacg ctatatgtgg caaatgaaat
     1261 attaattaat aggaggttgc aaaagatggc aaatcaatta tttcacattg tgattacaac
     1321 acattctacc ctttgtgagg gttatttaaa ggcaacaaaa ttaattttaa tgaatgacgc
     1381 cacaggtatt agtgctgttc catttaaaga aaatactagt ccagatgatt ttgaggagag
     1441 tatgaaagaa ataattagtc agcatcattt gcaacctcta attattttaa cggatttaat
     1501 tggaggaaca cctaataatg tttctattaa acagttgaac aacaatagaa ttcaaattat
     1561 tgctgggatt aatttacctc ttttactgga gttattaatg gcccagtcag ctggtaaatc
     1621 tatagaagaa atagatttta aggaattact gaataactct caaaaaagta tggttcatat
     1681 taattcgctt ttaaaagatg tcagttaagg tggcaatttc tattctataa attttaaaaa
     1741 ttgaaggagg aaatagaatg attaaattag tgcgtgtaga ccacagatta cttcatggac
     1801 aagtaatatt ttcttggact aaacagtatg atattaacca tattattgtg gctgataaca
     1861 aagttccagg agatccaatg tctgtgatgg ctttgacaat tgctaaacct gctgattgca
     1921 aattagacat tgttaaaatt agtgatgtta aagggattat ggaaacatcc ccaaatgata
     1981 attacatgat tttaattaaa ggacctaagg aagcttttga attagttcaa aatttaccag
     2041 aaattaaaga aattaatttt ggcggtgtgt ctaagaaaag cgagtccaaa caatatggaa
     2101 aggcagtctt tttaactcct gaagaattag aagccacaaa aaaaattctt gatcaagaag
     2161 tgaaaatatt tgtgcaacaa gtcccatctt caagtattga aaatgtagat tttagtaagt
     2221 aaggggggaa aataaatgtt attaaaagcc attttattag gtttagttgg tgtggtagct
     2281 gttattgact ccaggttaat tggtcgacaa aatattggta gaccactaat tttgagtaca
     2341 cttgccggac ttgtgctagg tgacttgcat acagggattg ttcttggggc atctttagaa
     2401 ttaatttcta tgggattcgt ctcaattggg gcggctggac ctcctaatat gcaacttggt
     2461 agtattattg ccactgcttt tgcaatttta actaatagta gtacagaaac agccttaaca
     2521 atagctatcc cagtggctgt agctggtgaa tttatgagta ttatcatgcg catgataatt
     2581 gctcaatttg gacatgctgc agataaagcc attgaaaata ataaatttag aaaagctcaa
     2641 attattcata tttattggtc atttattttt aatgcttttg tttactttat tccaatcttt
     2701 ttaacagtat attttggtgc tgacgttgtg gcagatttag tagataaaat tccagaaatg
     2761 attacagacg ccttgactgt ctctggaaat atgctttccg cattagggtt tgcaatgcta
     2821 ttaagcacaa tgttgagtaa aaaattatat ccttacttta tttttggatt ctttattgtt
     2881 gcctattcag gtctttcact aattggcgtg acaatttttg cagctctgat tgcctttgta
     2941 atggatcaag ttaaatatgg aaaaagagaa gaggcgcatg gttaatggaa aatagtacag
     3001 aaaaaaaacg tttaatcaca aaaaaagatc ttcataagat gtgtcttcgt tcatggacaa
     3061 tcatgtgttc gtggaattat gaaagacaaa tgcatatggg atttatgtat gggatggcgc
     3121 caattttaaa taaagtttat aaaaatgatg aagagaaaaa aagagaagct tatactcgtc
     3181 atatggcatt ttttaactgt acgcctcaaa tgactccatt tattatggga ctttgtgctt
     3241 ctatggaaga acaaaatgct caagcagaag aaggaaactt taatactgaa tctatctcca
     3301 tgattaaaac cagtttaatg ggtccttttg ctggaattgg tgattcattt ttccaaggta
     3361 ctttaagaat tattacattt gggattggat tgtcttttgc taagcaaggt agtattttag
     3421 gaccgatttt agctgttggc ctttttgcta taccttctat tttagctgtt ttttacggcg
     3481 gtaaattagg atatgagtct ggcaataaat accttactaa attgtatcaa gaaggtctta
     3541 tggatcgagt aatgcaattt gcttctatca ttggtttggc tgtagtaggt ggaatggtgg
     3601 ctagcatggt gccaattgtc acccctttaa aattcactac agggggaaca gaacttgttt
     3661 tacaagactt acttgattcc attattcctg gattattacc acttgtatgt actttgggaa
     3721 tatataaatt gattaagaaa aatgttagta caaatatgct cttattaggt tgtattttgg
     3781 cagggtttgt tttaagtata ataggtatct tataatgatt aaggctctaa tttttgatat
     3841 ggacggagtt ttaattgata gcgagattat gtatcaaaag tgggtattta attttttaaa
     3901 acaagaaaat ttaagctatc ctttgtattc ttatcaaaaa aagattggaa ctacagcaag
     3961 tatttttgat gatatagaaa agtacaatga gggatgtaac agtttagaat tgaagggaaa
     4021 atttactaaa tattggaaat cgcaaaagat taattacttg gatatttttc ctaatatact
     4081 aattgaacag cttgaatggt taaaaaagaa aaaaataaaa atggcaattg cttcttccag
     4141 tccaatggat actataaatg aagttgttac cacttgtaaa atagagtcat attttgattg
     4201 tttaattagc ggaagagatt tgccagaaag taaacctaat cctactatat tccttaaagc
     4261 ttcagaacag ctcagaattc cagttgaaga atgtctggtg atagaagatt cttataatgg
     4321 tataaaagct ggtaaaagag caaatatgaa ggttcttgca attaaagata aacgtttttc
     4381 tcaagatgta agtctagcag atgatgttat ttacgatatt aaatatctgc gacaagaagt
     4441 acaggtgaaa tttggaatat aaataagttt tttgatgtat ttagagaata ttagtaaaaa
     4501 taaaaatcat gcccaaatct aatcaaaatt gattgctttg ggtatgattt cttttaatat
     4561 caatctatta ctttaggatc tgaatcattt ttatgcaccg tatctaaagt gatattttct
     4621 gtgacttcaa taacataact aattctatca caacgaactg tggattgaga ataatgaaat
     4681 ggttgattat ttttatcttt gccaaccttt tctaaaataa atagcggatc tcctactata
     4741 cattgcaata agtcggaaag ctcaggtgtt gcagataaac cgtttatttc cataatagag
     4801 ctcgtagctt tgacatcata gtcatcttct agaagtttat ataatgaagt atattggcta
     4861 cctttttcaa ataaagttgg aaatttttct tctgatgcga aaaaattatc aattcccata
     4921 ggggcaccgt cttcccacat taaacgttta atattgtaaa ctctatcagt tacattgatt
     4981 tgtaatttat tagctaaaaa ctcatcagcc tttataattt tagaatgtaa aacagtatat
     5041 ctaattttat gtcctcgggt agaaagagaa tcgctaaatc catgaactcc agctaatttt
     5101 aaactttctt ttttttgagt tacaaaggtc cctttccccc ttaatcgttc aactgttcca
     5161 ctttctgtta aaagtttaaa agcttttcga atggtaatct tacttactcc ataaatttta
     5221 cataattctt cttctgtagg taattgctct cctggaagat attttttttc acgaatatcg
     5281 ttacgtatag aagcttctac ttgtagatat aatggtctat tatcttgaaa attcatagta
     5341 cactccctaa aaagttatac ttcattatat cacaatttat taaaataaaa gattaagata
     5401 taaatataaa tgaactagtc tttgactaac caatgcccgc ttttgaaaga aaacaaattc
     5461 attctgtttt aagtaaaaat ccttatgtta ccactcattc tgaaggggat gagccttatc
     5521 gctacttggt tgtggaacca agtgataaga caattttata aattcaatat taattaaaat
     5581 aaaaacaaac aaagtagatt atttta
//
LOCUS       DACDYJ010000118         5578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDYJ010000118 DACDYJ010000000
VERSION     DACDYJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.120"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(359..1342)
                     /locus_tag="IWM51_002670"
     CDS             complement(359..1342)
                     /locus_tag="IWM51_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP9972014.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            1720..3111
                     /locus_tag="IWM51_002671"
     CDS             1720..3111
                     /locus_tag="IWM51_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9972015.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            3127..4167
                     /locus_tag="IWM51_002672"
     CDS             3127..4167
                     /locus_tag="IWM51_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9972016.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDIMKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            4186..5271
                     /locus_tag="IWM51_002673"
     CDS             4186..5271
                     /locus_tag="IWM51_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP9972017.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            5304..>5578
                     /locus_tag="IWM51_002674"
     CDS             5304..>5578
                     /locus_tag="IWM51_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9972018.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYV"
BASE COUNT         1926 a          846 c          987 g         1819 t
ORIGIN      
        1 aaaagctgtt atgttgtgat tcgctcgcac ataacggctt tttatagttt tttgtcaaaa
       61 aaaggcagaa ggtgccgact caaaaaaata agtcgaggtt gggacagaag tgtttaactc
      121 cgagaaataa gaagaaattc acgaaaattg cttttcaaat ttttgtgaat ttcggcttat
      181 ttaagaagaa gttgcttctg ctcccaccgt ttattcgttt ttaaagaatg agacaaaagt
      241 gtttcttact tttgtcccac gctcttattc atgctcagag cgccaataga tacaaaatca
      301 tagccaaagt ttcacgaacc ctagctgtca aagaatcgat attttatttt aatataggct
      361 attttaaaat aaatggttta aggtattcct ctacaaaagc cactctatca attatcatgg
      421 gtttaaaata agaagtgatg gagataacca gattgttctt agcgtcaata taaagaacat
      481 ttccactatt accaattgct gaatatattt cgttctcaga atctaatacc caccataaat
      541 agccatatct catattttgt aagtcttcta aagcactggt tctaggtttg aacatctcgt
      601 ttatccacat gggtggtacg atttgttttc ctttatacat ccctttattt aggcataata
      661 caccaatttt tgccagatca cttgccgata aacacaaacc atatcctgcc gtcccgatat
      721 ttagtggatc acaaaaccag acattggttt taggagactt atccattgta aactgcttat
      781 gttcttcagc tgtttcagca taataattga catggtttgg tatccctata ggatcaaata
      841 gatacttatt ggcaaaatca acagttgtta gcttacttgt ctgatacaac aagccagtta
      901 atatgtgtaa acagacagtt tgatagttga attcgccggt tatacctttt cttcctccca
      961 ataaatctag tgatgttttt gtccaatctt cacttgaaca gacttttgtc catggatcgc
     1021 ctttacattt gtagggtgct ttcattgtta gcaagtgctt gattgtaata gcgtaaatag
     1081 ttttctcccc gcgtttcact ttatactctg gaaaataatc taatacttta tcatcgatac
     1141 ttttgattaa tccttgctct aaagcaatgc caatcagcaa agatacaata cttttcgttg
     1201 ctgacatgat atgacaagta tcgtcttttt tataaccatt ccattcatct gtataaactt
     1261 ctaccccatc cttatatgcg acaatttgac atatatttgg ttgtcgaaaa ccaataaaat
     1321 catgcaactc ttgtttattc ataagatatt acctcacttt cttctataag cataatgtca
     1381 ttaaaaatcg atttcaattt tttcgattct tcacgtaata caggtattct tatggactag
     1441 acagcgcttt gggaagatgg attctctgaa aaatataaag gttcacttta tgagtggata
     1501 aaaaaagcag caaatacttc gacttcttta atttaccaac attagtgaaa atcgattcat
     1561 ctggagattt gtaatttaga gtccttgtct tgattagagg tgtataaagc tttttattag
     1621 acatttttaa attccattac aatttgctta taaactaggt ttttaaaggt gactatggta
     1681 agataataga gatattattt tgtgattagg agtgtatata tgaataaaaa tggagaatgg
     1741 agtgctgcac gacggatatt cattatatta atggatgtta ttgtatacaa tgcagcaata
     1801 tatagtagtt tcttactgaa gtttcgtgga gagatcccat tacgaaattt agaaactttc
     1861 caacactcag caattattat ttcagtgatt tttattgcgt tgaacatttt gttgggtgca
     1921 tatgtttttt ataatcgaat gatcagtgac atcgtgtttg tcacagtcat cggtcaggta
     1981 ttgatgacac tagggatcat ggtagtgact tttgcgggcc gctggtttgc ttttccacgt
     2041 cttgttatct tactttcatt tgttctcggt acgcttttct taagtatcta tcgtattctc
     2101 gtatataaac tttatttacg cgtaagtcga gataaaaagg tcgtcattat tgggttagaa
     2161 aaggatgtcg caccagcaat ccaaaatttt ggttcaaaga aaaacaacaa acataaagtc
     2221 gaagcagtca ttcttgataa ctattatgca aacatcaaaa atatgattaa agacatcgat
     2281 attgtctatc ttgcttctca tatcgaagaa actgaaaaaa tcaaaatcta tcaattagtt
     2341 actaaaaatg aaaaaaaatt gtttttgaat acaacatttg aaaacttgac attgatcaat
     2401 ccaaatatta tgaatttcga agatgaaagt attattgaag catctggatt tagaatccaa
     2461 cctgaatatg aactattcaa acgtttgttt gatatcatcg tttcacttgt tttattagtt
     2521 attgcttcac catttatgtt attcacagca attttagtca aagtgacatc accgggacca
     2581 atcatctata aacaagtgcg gatcacaaaa aatcaaaagg aattttcaat ctataaattc
     2641 cgctcgatga ccgccacagc cgaagcaaaa tctggaccag ttttagctaa gagcaatgat
     2701 gcacgagtaa cgcctgtagg taaatttatt cgagctgtcc gctttgatga attaccacaa
     2761 atcttcaatg tgctgaaagg cgatatgtcg atcgtaggac cacgtcctga acgtcctttc
     2821 ttcgtcgatc agttcaatgc agaaaaccct tattactact tacggcataa tgttcgtgct
     2881 ggtatcactg ggtatgcaca agtctatgga aaatatgtct ctgattacaa cagtaaatta
     2941 cgttttgatt tactttatat caaaaaatat tcattgatgc ttgatttaaa gatcttgctg
     3001 caaaccatca agatcctatt tgataaaatg tcctctcaag gacttgaaga agacgaaaat
     3061 gtgatctcat tggcagaaat tgcttttccg aatgaaaaaa tctttcgcta gttgggaggc
     3121 gtaaaaatga acaatccgaa attcagcata atcgtaccag tatacaatgc agaagcaact
     3181 attggtcgaa cgattgatat tctaaaagaa ttggattatg aagagtacga agtcgtttta
     3241 gtaaatgatg gatcaacaga tcaaacacag gaaatcattc aagaactgat tgaaaatgat
     3301 gaacgttttg aattgattac tacgaccaat caagggcccg gattagcaag aaacgaagga
     3361 ataagacagt caaaaggaga atatctctta ttcttcgatg cagatgacac gccagtaaaa
     3421 acgatcttgt cagattatga tcatttgcta gaaaagagtc caaaagcgga tcttgttgtt
     3481 agttcgtttg tttttcgtac aatggacgag ggaagaatcg tttccgaaaa agaaaactta
     3541 gttacagaat gtcactatct aactcacgaa gagtttataa aagacatgta tcagttaatg
     3601 aatgaacagt tgatgtatgt tgtatggaac aaatgctatc gtagcgatat tatgaaaaat
     3661 aaccaaattc tatttaaaac ttataatagt tgtgaggatc gaatatttaa cttacattat
     3721 tatgaatact gtcatcaagt gatcatgaac ccaaaagtag aatatattta tgaatttgaa
     3781 ggtgggaagg ggatcactaa tcaataccga cctaacaagt tcgaaacatt taaagaattc
     3841 tattcattag caaatactgt aacagacgat attaataaag acggtatggc ggcgcttctt
     3901 ttaaaaggga caacttcagt tattttttct atttatagta cctctgcaag aacttcaaaa
     3961 gagaagaaag acgaagcaaa gcagatattg agtgatccag tgatccgaga agccaagcaa
     4021 attgctaaga cggattcttc agcaaaaaaa ataacaaagt tgctttacaa tatgccagct
     4081 ccactatttt tatctgctgt aaaagttgga agcttcgttg aagtgaaaat gccagggtta
     4141 atggctgctt tgaagcgagt atattagtta atggaggaaa atataatgaa aagaattttg
     4201 cattttcagg gacgaatggg tttaggtgga gcagagtctt tcatgatgaa tttatatcgt
     4261 aaaattgatc gtacaaacta tcaatttgat tttttgattt atgaagatta tgcagatgtt
     4321 caagactatc attcagagat tgaaagatta ggaggccgta tttttgttgt acctaatcct
     4381 aagaaaaata tttttaaata tttaatagaa gtgaataagt tactaaaaaa agaatctttt
     4441 tctatagttc acaatcaggt ttattttggc gggggaatta atttatggtt agccaaaaag
     4501 aatggaatca gacagagaat tgctcatagc catgcaactg aagatggaaa atctcaaaat
     4561 attgtaatga atgtattgcg taaattttta accaaattgt tgttacaaaa tgcaacagac
     4621 taccttgccg ttagtcaaga agctggtgaa agtctgtttc aaaaccatcc ctttgagata
     4681 gttcataatg gtattgattt agagctttat agtaaaaatt cagaagctaa ggttaataag
     4741 agaaaagaat tggatatctc aatgagtact tttgttgtgg gaaacattgg acgattagag
     4801 aaacaaaaaa atcaatctta tttgttagag atttttttag aaatactaaa aatgaattct
     4861 gattcttact tgattttagt aggtgaaggg tcacttagaa ataaactgaa agccaaagca
     4921 gtagaattgg atatagagaa aagagtaaaa tttttaggag aaagaaacga tattcccgag
     4981 ttattatctg tgatggatgt ttttgtaatg ccttctcttt atgaaggtct gccaatggtt
     5041 ggattagaag cacaagcagc aaaattaaaa ttagtcttag cagatactat ttccaaagat
     5101 acaaagttaa caaataatgt ggattttgta tcattagatg aaacaccaca agaatgggca
     5161 aaaaaagtaa taaaagagcc gtatgctaat aaaataacac aagaactaaa gagttatgat
     5221 gtttcgtaca ctactgaaca aatggagagt atatatagta gagatcatta aaaatgttca
     5281 agatatttta ggagtgatta tttttgaaaa tatgttattt gatattggca tacaatgata
     5341 ttggcaactt aaagagattg gtcaatcgat tgaacgaaaa tgcagttttt attattcata
     5401 tagataaaaa aactgatata gaaccattta ttgaagcatt taaatcatat gataatgttg
     5461 aatttttatg taataaaaac cgtgttaaaa tttattgggg tggtttttcc attgtacaag
     5521 cagaaatcaa tttagtaaag agagcattac aaaatgagaa atatttaaaa tatgtatt
//
LOCUS       DACDYJ010000119         5522 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDYJ010000119 DACDYJ010000000
VERSION     DACDYJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.121"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            227..1528
                     /locus_tag="IWM51_002675"
     CDS             227..1528
                     /locus_tag="IWM51_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9972019.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1555..1815
                     /locus_tag="IWM51_002676"
     CDS             1555..1815
                     /locus_tag="IWM51_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9972020.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPEIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2814..4049)
                     /locus_tag="IWM51_002677"
     CDS             complement(2814..4049)
                     /locus_tag="IWM51_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP9972021.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            4617..5444
                     /locus_tag="IWM51_002678"
     CDS             4617..5444
                     /locus_tag="IWM51_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9972022.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         1707 a          838 c         1102 g         1875 t
ORIGIN      
        1 atatgtttca atcactagat ttaatcattt tgatttttgt taattatcga atttttttgt
       61 tgctcctatg atttagaaaa cttattttca ggattcttaa aaataaattt gattatttaa
      121 gagaatatat tgaaataaaa tttcttaatt atataattag ttatgaacat tctttgcgtt
      181 gtcatgcaat gaatggttca tattttattg aaaaggagtg gttttagtgg aaaagctgat
      241 gaaatgggtc gaagagaaac ttgtcccacc aatggcaaaa attggtacac agcgtcattt
      301 attagcaatt cgtaatgggg ttgtttcaac attgtcattg attttgattg ggacattttt
      361 tatggtcttt atcaatttac cgtttcctgg atggaatgaa tttattgccc cttattcggc
      421 aacgattgtt ttacccttta ggatgacgat gggattgatg gctatttatg caacttttgt
      481 tatgggttcg gatctagcta aatcatatgg attagatagt gtgacaggtg gtatattatc
      541 tttaggaacg ttctttatgt tacaagttcc tgtaaacgtt ttaactcctg aagaggcacc
      601 tcttggctgg gtcttaccta tgagttcttt gggagcatct gggatgtttg cggggatctt
      661 atctatgatt tttgcagtgg aagtatatcg tttctttaaa aatcgaaata ttactatcaa
      721 aatgccagaa caagtgccac ctgctgttgc ccgtagtttt gaggcattga ttccaggtgc
      781 agtcatttta acaattactt ggctgattcg aagcgtcgcg ggctttgatg taaacgctgc
      841 cttattatcg ttattcgagc ctttaaccaa tattttaggg aataatttgc ttggtgtttt
      901 actaccaatg tttttgatcc atttgttgtg gtcatttggt atccatggca tgagtattgt
      961 aggggctgtt gttcgtccaa tgtggctgat catgttggat gagaatgcaa aggcattggc
     1021 agatggtaca gctgccaccc aattacccta tatcgctcct gaacagtttt atcaatggac
     1081 ggtaacaatt ggtggtgcag gagcaacgat cgtggtatct gttttgttta tattcttctg
     1141 taaatcaaaa ttcttgaaag aggttggtcg tttttctatt attcctggta tcttcaatat
     1201 caatgagccc ataatttttg gagctcctat gatgttgaat ccttacatgt tcatcccgtt
     1261 caacttagtt cctttagttt tgactattat cagttatttt gcagtcaaat tagaaatggt
     1321 caatagattt actgttttgc caacatggac attaccagct ccaatcggcg gttatctttc
     1381 agcaggaaat gactggcgtg ttgttatctt gatcatgatc aacacattga tagcatttat
     1441 tatctattat ccatttgtaa aagcttatga taaaaagatg ctgtcagatg aacaaagtcg
     1501 tggagaaaac gaagtccaga ctgtctaagt ttggttttta gaaggtggga aacaatgtat
     1561 tggctgattt tattttttgt ttttatcttt ttattaacgg ctagtcacct tatattgaat
     1621 atgctagcta catatcatat tcaaatcaat cgatggattt gggcactggc atcatttttg
     1681 atcgtgattc ttccagagat cattgtaccg catatgaatg ttttgttttc atgggggaca
     1741 tatgtacttt gtggaatttt tgctatcaat tttatgatcg agcagcatag atggtttgtc
     1801 acatcgaagc tttaaatgtt ctaaaagtgg ttgtaacaga agtggttagc ttcaagaaat
     1861 aagaagaaat ttacgaaaat tgcttcttaa atttttgtga aatgttggct tatttttcgt
     1921 ttggttgaac ttattaccgc agatgcaagc ttttgaccac cgtttattcg gaatatggga
     1981 ctgtgacaag gcttttgtca cagccctttt ttacataaag attattattg ttttaactgt
     2041 tttggcagaa agaaggagga aaaaatggaa attttatcag caatggattt agaattagta
     2101 gatctactac caaagtgatc aagtcaaata tagatttggg agcttacaac catatagtag
     2161 gggctgcagt atggtgtatg aatggtaata tgtatgatgt gagtgtttat tcaaatcatg
     2221 aaacgtgtat agaaataatt gtgctgggaa tagcaatcag tcatggtgaa cgtgactttt
     2281 agtgtatcgt tgctgacgac atagaacatt ctgacatgat ctactcaata tgtggggatt
     2341 gctggcagtt tatataggag tatgcatcgg attgcgagat cattgtctca acagaagagg
     2401 gaggttgatt atcaaagata aagtggtaaa tcctacattt gatttagtaa ttgttgaggg
     2461 gactagcggc acaaatctct acaaagcgat gcttctagat ctatatgaca cattttaaga
     2521 aaatgtattt gcctaattat tatggtcttt cttttgaaga aacgttgaga tgatccaacc
     2581 agagaaaggt gtcaaagaat ttgactaaga ataaatggca gacgctacct atgctcattt
     2641 ggatagcgtc agatctttta ttttaaggat gattttgtgt tttttagaca ccgtaattta
     2701 agtaactcta acttttcaat ccactattta ctttttttgt attttcactg tctgggtctc
     2761 gaatagcgaa attactgatg agtctctatt aaaaaagctt tttctacttg aaactatatt
     2821 gggtggttgc tcaggaaggt ttcgatttct tgaattgctt gttgtggacc atttattgga
     2881 aaaacggctt gcagcgtgcg tgcctgctga cgaatcttgg gtgaagaaac ttcgcgcaga
     2941 gcaatctgca gttttctttc agtcactggt tttgtgaggg cgattgcgtt tccgtatttt
     3001 acacaatagt caatattaca gctttgctca aattgcatcc ccacaccgat aaacggtttg
     3061 ccggattcac aggcagtttg gacggtccct tccccgccgt ggataaaaga aaaatcaatg
     3121 aattcatgca aaagatgact agacagatat tctgttatta aaagatcttc tgagtcagat
     3181 tgaatgtcat caagcagata gtcaataggt gcaataaccg ctagatccgg ttgtttttgt
     3241 aagaagaaca aaattttgcg taacatacgc ggattgcctg aactacccat tgcgaaatag
     3301 ataatacgtt ttctttcttg ttgctttcgc tgtataaaag ttagcacttc ttcaggcaag
     3361 gaagtactga gcttagcaaa aattggacca acgattcgat aattttccgg cagctgttct
     3421 tcttccaaaa atagtttagg cgttgtgatg agatttagat cagcatccat catttgtaaa
     3481 cttgtttttg gtaaaggaag catttctttt tgtgcagcgt gtttgaaggc attggggatc
     3541 catcgggtat ttactatacg cttttttgtc cagtgccaaa ggggattgct taaagggccg
     3601 aagaaacgta actcgtgggg catcagcgca tgctctgatt gaaaaaaagg ttcactcaaa
     3661 gcatagggtt taacatatac taaaggaatc tttttattgc gggccgataa aaaaattgtc
     3721 acattcgaac ctgtcacaat cagtcgaggc tcaaacttag caatcagact ctgttcacta
     3781 gtgatacgag aggcaaccat ttctttggta aaagggtttt taagtgtacg caactggtca
     3841 aattttatga tttgttcttc ttgttttttc gtaaggatcg gcgctagtaa atgatactca
     3901 aagccacttt ctgtgatcaa aggagcaaaa gttttagaaa agccaaagaa tacacagtaa
     3961 taacggtctt tcatagcttt ggcaacctca atcattcgtg ttgtctcagc taaattgaat
     4021 actgcaggag caaaaagaat tgtcttcatg aaatcctcct tattggaatg agtctatccc
     4081 ttcgtttctt actagtatac taaaaaacac agcgttcgag tagcctatgt atattttgaa
     4141 tattaaactg tctctcgctg ttttcacaac ttctaaattt aaattttgta atcaagttga
     4201 aactccaatt ttcaaaaaaa cacgcaacaa ttttttgatc gatttatatg ttgtgccaga
     4261 taaatacgag aagttgtgtc attaaaaata aacatgagaa cataaaaaaa taaaataatt
     4321 atattaattg ataggtgtgc tgtcgtcatt atacacacgt aagaaagaaa tagttatgtt
     4381 taaaaatatt cttagacttt ctcgccatga caattttcac tatgtgttat aactgtaaac
     4441 gataattatt cagataacac aaaaagaaat caaggagcag atacgccagt tgtttaattt
     4501 gaatattatt ttaaaattaa aatttttatt ctgttctaaa catttttggt tgtttagaac
     4561 ttttttttgt gtatgatata aaggagaaat aattttttaa agggaaggaa attgttttga
     4621 ttaaagtaga tggagcatta ctaaaagaat accgaaagaa aaaggggtta acacaagtac
     4681 aattagcaga aggcatttgt gaacaagcaa ctgtcagcaa tatcgaaaac aagaatatgt
     4741 cgccaggcat caatatctta agtgcgcttt gcaaaaagct gaatgtcgag atctccgata
     4801 tattagtaga aacagaagaa gacaaaatca ggaaaaaaat aaatgagatt caatcattga
     4861 attctgtatc aaaataccaa gaatcttata aaattttaca aacgatcgat caggaaaaag
     4921 taagtaattc tgatctttta aatcaaattt tattttatca aggactgctt gatcatcttt
     4981 atgcgaaaaa accagatgca gcattatttt attttaatca agtgttggaa caaacgaccg
     5041 aaacagatat ccatcactta caagcagctg caaacgtggc aatgatctac ttgtcaaaag
     5101 gagaattaga ttttgcgaaa gtttatgtgg agcgtacact taaaatattg agtgagactg
     5161 attttgataa tctgatggtc tgcattgtat attatgatat cgccacctat taccgtaaaa
     5221 tcgaggatta tgataaagca atccaattat gtgagaaggg tattgagtat aataagaaac
     5281 ataaatcgac ttatgcattg gaatacttgc tttatgaaat tgcttcttgc cataagcaat
     5341 taggagaaga tgattattta gaaagatata tggacgcaaa aaaaatagcg cgtttcaacg
     5401 gaaatgatta cgcggtaaag gtgattgaaa acgacctgaa ataaagtggt tgtgaaatca
     5461 gtgttctgat ttgaataccg tttattcgaa aaaaggaggt gtgacttttg tcatacctcc
     5521 tt
//
LOCUS       DACDYJ010000120         5429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDYJ010000120 DACDYJ010000000
VERSION     DACDYJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.122"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(151..426)
                     /locus_tag="IWM51_002679"
     CDS             complement(151..426)
                     /locus_tag="IWM51_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP9972023.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(398..1351)
                     /locus_tag="IWM51_002680"
     CDS             complement(398..1351)
                     /locus_tag="IWM51_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9972024.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1963..3456
                     /locus_tag="IWM51_002681"
     CDS             1963..3456
                     /locus_tag="IWM51_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP9972025.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3591..3887
                     /locus_tag="IWM51_002682"
     CDS             3591..3887
                     /locus_tag="IWM51_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9972026.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3988..4839
                     /locus_tag="IWM51_002683"
     CDS             3988..4839
                     /locus_tag="IWM51_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972027.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            4860..5072
                     /locus_tag="IWM51_002684"
     CDS             4860..5072
                     /locus_tag="IWM51_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972028.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1869 a          836 c         1014 g         1710 t
ORIGIN      
        1 tttagtatag taaaaactag aaacaataaa atttgtgaat gttgtatacc cccaaacaat
       61 acacatattt tgaaggatta ggattaataa agctaaaaat atagtaagta tactaaatat
      121 atttactata tctagtatac ttactatatt ttaatctttt gatcgtctat tatatataat
      181 ccgttcctcg tcgtttaaag tatcaacata ggcgtccatt gcaagtttga ctatttcata
      241 cataggaata tcttttgcat aactaagatc ccgaatggtt tttagtacgg gaggatctac
      301 ttgaactggt tttctttctt tcgcagtgta ctttcgttcc cctaactcga cattaatatc
      361 atctttaatt gaaatatctt cagcaccaat tttcttctta tctttgttcg ttggtaataa
      421 tgccatttag tttaatctcc tttccaagag gtaggatatg attggccatt gagtcagcat
      481 atttaggtgt ataatcaaat ccttcaatat caccatgttg ttcatagtat tcaattcttt
      541 cttctaattc tgtaaagatg tctgcaaaaa tagcccacca tcttcgatct acataatcat
      601 ttaattggat accattctca ccatacattt ctaaccgatc cgagccttta acaatgcttt
      661 taaaaatgtt tgattcacca tacttatcaa ttgttttttg atagtttgcg tgttgagagg
      721 aacgtcgcac tgagaaaaga acaggcaaaa ttccaatgat ttgaagatga gactcttcaa
      781 aattcgtgac aatcggaact aaaactttat taataaaatc ttctgttcct tccattgcgt
      841 accgttttaa ttcttgcatt gcaataatat aatctgcagc agttaagaat gcacgaacca
      901 cattatctgt tgatggtggg cagtcgataa agatataatc gaaatttttt ctcaattctt
      961 ctaaaggttt aatgaaggcc aatgatctct tacgacgatc aggatatttt tcggacaagt
     1021 attcattaag ttcttcagat ccaggagagc cagccataat atataaatgt ggacttactt
     1081 gagtaatacc tgaaacccag tccccttttt tgattgctgc agacatggat cgatcaaagc
     1141 tagtaatttg atacgttgat ccaatgttgg aagtagtatt tctttgttgg tcataatcaa
     1201 ttagcaacac gtctttttca aaaagttgac tagaaacaat cgctaataaa tcattgtctg
     1261 tagtcttacc aatgccgcct tttctacctg taagcattac aacttttcct agtttatctg
     1321 atgttttaaa tgcagtccgt aagttttcca tagttccctc caatttccta tactaaatat
     1381 atttagtata cttcatatac ttaatatatt cgatatatct aatatactcc aaattgataa
     1441 gaattcagac tttataagga atatagtgga tatactaaat atatttgcta tattcagtat
     1501 atcagatata ttcaaaatgt caaaatatat ttgtgcgttt tgcgttgaca atttagagtg
     1561 aaaagaggta aacttaattc tatagaaacc acagataatt cactatttac aaagtagcaa
     1621 aagcagtggt actctttaaa cagaaaaatt acataaaaaa aagagacgga aataattgat
     1681 tttagcagaa tcaattattt ctgaatcaca tggttaggac atgtgttcac gctcgtctca
     1741 agaatatgta agtagctacc aactacttat attaaaagtt gatcggatac cttttccgat
     1801 tgcctttatt ctaccaaaaa aaggcgagca actcaacaga agaaagttta tttagtgtac
     1861 ttaaattaaa tattcttaaa cactgaagag agaacacata ctatggctga gattaaagct
     1921 aaactgtgtt ctctttttgt ttgagaaagg acgttacaat cgatgttgag cgaaatttat
     1981 tcagctattc ttaaaaacgg cttacgtaca tacaaatatc gaaacagcac catgaaacct
     2041 gcaggtcata ttgattccag taggagtggt gctatctttg cgtaccgtag caagcaatta
     2101 atgaatgttg ggcgtggtat ggtcattact tcggaagaag ctattttaga gaacgaaaag
     2161 aaactaacgc attggacacc taatacctat cgctttggta cttatgcgga tgattatcga
     2221 acagtggtta aaggtcattc tgagaaaaac ttaagtcaaa tcaacacatt tgtagttgat
     2281 atcgattcaa aagagaacca tcaaggcgaa attattttag cctgtttaga tcaagtgggc
     2341 tacatgccaa cgctaatttt agaatcagat catggttatc aagtttattt tgtactaaag
     2401 acaccaggct atgtcacaaa gaaaagcaat ttcaaggtga tcgatgtagc aaaaaaaatt
     2461 tcaaaaacaa ttcgtctaca gttggctgaa aaattgtccg gagtggatct aggatgtaac
     2521 cacttcggaa ttgctcgatt cccacaagaa aataatattg tttttaaaga aatgaactat
     2581 cagtattctt ttgctgactg gattcaatgg tcgatgaagc ttgaatcaga tcaaaaaaca
     2641 aaagaagatc gagaacgaaa actaattgtc tttcctgaga aaaaagaaat tcgtcaagta
     2701 gatgaaccgt ggttcgatat gttacttcat aaagcaaaca tcgtcggcgg agaaggacgt
     2761 cttggtcgaa ataatgtgat tttcacgctt tctttagctt attactcttc aggttatgca
     2821 ttggatactt gcgaatacaa tatgttcaca ttcaacgaac ggttacagga accgctatct
     2881 gaagcagaat tagtaaagat tgtacgtagt gcttattctg gtaattatca agcagcaagc
     2941 agagaattca ttattgaatt gtgccagcaa tgggtttcac caaccttaaa aaataatgat
     3001 ctgttcattc aacgacgtgg ctggtggaaa tttaaaaagc cacgagatca aagaaaatat
     3061 agtcataaaa atgaatggaa agaagatcta ttggcttatc taagcgaaaa aagtcttgct
     3121 agtgagcctt atttgaacct gtcaaaaaaa gaattggctt cacaactaac gatgcccaag
     3181 cgaacacttg ataagatctt aaatgagtta cgagaggaga ataaagtctt ctacaaggta
     3241 acacgaggaa gaaatggcgg aatgatcatt gcttcagtca aaacgttgtt cgcgagggtt
     3301 attcaactaa gtaaagaaaa gagactagat tattcaacaa ggatcacaga ggtatttgga
     3361 ctcaacgaga gcttagtaga agagacattt agacaattat caaccccctt gaaccctctt
     3421 aaagaagtga atctttttga agtagataca ggttgaaaaa taaaaggtgc aaattgccct
     3481 atacaatata tctatgatac ttctgtaggt gttgctccct ttgcctagct acgtgtaggt
     3541 ggtatactta taactgcgta tgcagtctta tgtatcatgg aggaagttgt atgtcgttaa
     3601 aaaattatgt ctattcacat cctgttaatg tgtttatcat tggtcgtttg ggattatctg
     3661 tggaagtgtt ctgtgagctt tatggtttta agcaggggac tttgtcttct tgggttacca
     3721 gggaaaaaac agttgcttct ttgccgattg agtttcttca tgggttgtcc ttagcttctg
     3781 gggagactat ggatcaggta tatgattgtt tgtgtgtgtt agagcaggag tatattgagt
     3841 ataaaatagc taacgagtta agaaaaagga agaagtatat ccagtagtag tttttattta
     3901 cttaaaaaat tggttcattg agcaacctat cgctgctaaa gtagcaaagg aaaacgttaa
     3961 aggaaagaat ttagggaaga aatcgaaatg aaatcggacg actggattga acacacctgg
     4021 cttcctctga aaagaaatag caaacgaaag aaattttggg tattgcaatt atggccatta
     4081 ggaaaacttt gtagttacgg ggcacgactt attcttgatt taaccgttta ctcgattgga
     4141 gtatttggaa cggcttatta cttgataaat gatcccaata ggattgcagc atcagacatt
     4201 tttacagtgc taatgacgtt gatttttgtc atgctattgt ctatttttac tcaattagtc
     4261 atgcaattta ccaggcaaaa attaacagca ggaaaacttt tcaaaagcct attcgattta
     4321 attgttgtaa atgcaattct agcgacttta ataaaacaaa ttgaacaatc tggaagaaca
     4381 acaaaaggag tgaaggattt tttattgacg tttattcact ttgtaaatgg acacatgtat
     4441 ttgtttgaag tgggtattat tctctttttt gtcgggtatg tatccacgaa acttattaaa
     4501 cgttcaattg aaaaagaaat tgaaattgac ctgattccga cacttcttgg ttatgagtac
     4561 aatgttgatt ttggaagata tgacgaattg atcaatacgg atatcgttca cacgagtatc
     4621 catgaatcta ctgttaaggg catcagatct attttaaaaa atggccagga aatgaaagca
     4681 atagatcgaa gaaaagagat ctacaatttt tatgaagtcg aagcaagcct acgatatggc
     4741 atcggaaaat tgtggttaga tggaagtaat attcgtcaaa acaaaccata cattttttgg
     4801 aacagtagtg atttagaact tccaatgaaa gataaataat tatggagaat cgtttgaaaa
     4861 tgattcctct atacaggatt gtgcttatag ttggcatgga gttaaaaacc ttaaagaagg
     4921 agcagttatt caaagtgaaa gaacgtcata atcagttact atgtagtaaa acgggtacga
     4981 tgaacattga ataccgaaca gaatatttag aattaacgaa tcaaactgat tttttgttga
     5041 ttcgaaagca acaagagcaa gaaaaaaatt aatggctact tatagccaat tgattgatga
     5101 atgggaacaa ttcaaggaat ctaaaattcc agaacgtctc taaaagcctt tatttgtaaa
     5161 gagcataagg aaacgtggga acacttttta aaaaaagtgt tccaataggc tgtgcctatt
     5221 acgtttctca aaaataaatg tgggaacaaa atatttaaaa ataggatagt gaaaggatag
     5281 tgaaggatag taaaaggagg gtagtaggat agcaaaaagg ataggctcct tttcgctatc
     5341 ctttttaaat tggaggaatt aatatggaag taagaataag aaatgtggat gagaaaatcg
     5401 taagagaaat tgatcgaaga tgtgacgaa
//
LOCUS       DACDYJ010000121         5403 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDYJ010000121 DACDYJ010000000
VERSION     DACDYJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.123"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..480
                     /locus_tag="IWM51_002685"
     CDS             <1..480
                     /locus_tag="IWM51_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9972029.1"
                     /translation="SRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKMVYQASS
                     KEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIESFNKQ
                     IKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQPMEN"
     gene            1016..1588
                     /locus_tag="IWM51_002686"
     CDS             1016..1588
                     /locus_tag="IWM51_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP9972030.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            1820..2437
                     /locus_tag="IWM51_002687"
     CDS             1820..2437
                     /locus_tag="IWM51_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006845390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9972031.1"
                     /translation="MNYIAEKAHTKQYQKGEFLFRSQEKEDTLYIINRGKIRIYRLAD
                     SGKEQLVRILNPGDFTGEWTLFNPDAVHEEYAEATRDTVVCMIQQKDVQDFLEQYPAI
                     SLKLLAEMSKRLESSERQTTQVAVESVITRLVLFLAENVEPEMGNSPTITLPMAKKDI
                     ASYLGTTPETISRKFGELEDEGLIQQLSGKRIKIQDLDDLLLYSE"
     gene            2464..2754
                     /locus_tag="IWM51_002688"
     CDS             2464..2754
                     /locus_tag="IWM51_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003142317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP9972032.1"
                     /translation="MFCRSLLTTGQNSVLLRQGEVALSYVLWYSVGRFFIEGMRTDSL
                     WIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQKKTIKMVN"
     gene            2901..3134
                     /locus_tag="IWM51_002689"
     CDS             2901..3134
                     /locus_tag="IWM51_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HAP9972033.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGVDKDSIKVLFNSSKV
                     KANFDSAVTSIEEIQKAIENVGYPVLKAKVKAA"
     gene            3332..5236
                     /locus_tag="IWM51_002690"
     CDS             3332..5236
                     /locus_tag="IWM51_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9972034.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAVPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDIKRFEQHGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGHMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
BASE COUNT         1787 a          921 c         1175 g         1520 t
ORIGIN      
        1 agtcggttct atcccaaagc tcgttttcaa cattgttgtg tacacgtttt tcgtaatatc
       61 agtcacaaag tgcgtgtcaa taatcgtaag gaagtctgtg atgattttaa aatggtgtat
      121 caagcctcat ctaaagaggt ggcgttagaa gcacgtggtg cttttgcgaa aaaatggaaa
      181 accagctatc caaaagtggt tgaatcgatt ctttcgaacg atcacttgct cactttctat
      241 gatttcccct tggccatacg caagagtatt tattctacga acttgattga atcctttaat
      301 aagcaaatca agaaatacag ccaccgcaag gaacagttcc aaaacgaaga gtcgatggaa
      361 cgtttcttag tctcgtcttt tgatacttac aaccaaaaat tcctaggtcg cagtcataaa
      421 ggtttccaac aggccgaagg cgagctcgaa caaatgctga gtcaaccgat ggagaattag
      481 aaacaatcta cagtagggaa gaaccattta cacaaaatta ttgacgctcc caagctaaaa
      541 ctcctattaa tcgatctact aaaaataaat aaaaaaattc ttttcaattt ttatttatgg
      601 tatgacataa ataaagtaaa gttattcttc taggttaatc ttattaatac tctttctcac
      661 tagtgttgca tgcaacacta gtgagaaaga gtattaaata tactgcaagg caagacattt
      721 ggtaattgaa gaataacaag caaaaaaata gcagtcaggt ctcttgacaa agtatagttg
      781 ctattctttt tttaggccct actttaatga aatttttgtt tggagggaac gacttcattc
      841 ctttgattat ttcagttgaa tttaatagag aagcctggcg tctattaaga aatacttttc
      901 ttgatgagcg tcaatttttt taacggaaat ctatcctata ctaaattcag taaagaaaaa
      961 ggaggaatca aagaaagcta ttttaaacaa accaaaatta taggaggaat aaatcatgac
     1021 tgaaaataaa gctactcaag aaagaacacc ccaagaaaga ttacaagaag aacaagaaca
     1081 caaggaacat gttcatcata cgaaaattaa tgcagcggcc attgcggatc atcttttagg
     1141 caacatgcat acattacatg tgaaactgca ccaatatcac tggtatgtaa aaggagccaa
     1201 tttctttacg ttgcatgaaa aatttgaaga gttgtataac gaaaatgaaa aatggttcga
     1261 caaacttgcg gagcgtctaa ttacttctgg gcataaacct gcttcgacaa cggttgaatt
     1321 tgaaaaatat tcgatgcttt cggaagatgc agttaataaa tacgctaaag cagaagacat
     1381 ggttgaaaat attatcgagg acttcagaag cacccgtgac ctaactattc gcgcgattcg
     1441 tttagctcaa gacgagggtg acgatgcttt agaagataca ttgattgctt ttaaaaatga
     1501 cttagacaag aacatttgga tgctgcaagc gttcattggt aaagaagcat tagaagatga
     1561 tgacgctctt gatgaggatg aagattaagg taaaagtaga agctgattta gggattggaa
     1621 ttctttccag tcctttttct attttcttga tgtgcatcaa tttttttgta gtaagagact
     1681 tatataataa aagcagcgaa tataaacaaa ataaataaag ggggagaatg aattgactaa
     1741 acatacccat catgaaattg gcgaccacgc agcctgtatc cgcttggttc caatattcaa
     1801 tcacttagag gatagcgcga tgaattacat tgctgaaaaa gcccacacga aacagtatca
     1861 aaaaggagag tttctgtttc gttcacaaga aaaagaggat actttatata ttatcaatcg
     1921 tgggaaaatc cgtatttatc gattggcaga ctctggcaaa gagcagctag tgcggatctt
     1981 gaaccctggt gatttcaccg gagaatggac cttgttcaac ccggatgcag tacacgagga
     2041 atatgccgaa gcaacccgag ataccgttgt ctgtatgatt cagcaaaaag atgtccaaga
     2101 ttttctggaa caatacccag ctatttcttt aaaactattg gcggaaatgt ctaaacggtt
     2161 agaaagttca gaacgtcaaa cgacacaagt agccgtggag agtgtcatta cccgtttagt
     2221 tttgtttttg gcagaaaatg tggaacctga aatgggcaac tctcccacca tcaccctgcc
     2281 gatggcaaaa aaggacattg cttcctattt aggaacgaca cctgagacca tcagtcgcaa
     2341 atttggagaa ttggaggacg agggattgat tcaacagctg tctgggaaaa ggatcaagat
     2401 tcaggattta gatgatttat tgctttacag cgaataatcg gacacacttt tttctacacg
     2461 ttggtgttct gtcgctcttt attaacaacg ggacagaact cagtgctttt gcgtcaagga
     2521 gaagtcgctt tgagttacgt tttgtggtac tcagtgggtc ggttcttcat tgaaggtatg
     2581 cgaacggaca gtttatggat tggcgagtgg atccgcgttt cgcaagcctt gtctctggcc
     2641 ctgtttattg gcgcgattgt agtatggttt atacgcagac aagattatcc accaatttct
     2701 tattattctg atggcaataa ggggcagaaa aagactatta agatggtgaa ttgagcaaaa
     2761 agataaaaag aagtgcaaaa cttgatatgc gtcaattttc gtttgctaaa agggagctat
     2821 actacagttg taaagaggaa aaggcagtgt tcattaatct gctgccgata aaataaaaac
     2881 acataaagga gataggaatt atggaaaaag ctatattgca attagaaacg ttgtcttgtc
     2941 caagttgtat gcaaaaaatt gaaggtgccg taaaatcagt taacggtgtt gataaagaca
     3001 gcattaaagt attattcaac tccagcaaag tcaaagccaa ttttgattca gctgtcactt
     3061 cgattgaaga gattcaaaaa gctatcgaaa acgtaggcta tccggtcctt aaagcaaaag
     3121 tcaaggccgc ttaacagatt aaatgaacta ggcaattctg gaagaaaagc tatttttcca
     3181 ggattgcttt ttttgttgca atccgattcg aggtgcctag aaacgataaa agagaacttt
     3241 ctaaacttga tgtcagtcaa ttaattactt aggcaattat cttatactgt gtttatcaat
     3301 aaaagagaaa ttaaatttgg agggaaaaag catgcaacaa tatatattaa gcaagaaaaa
     3361 tgttatcacc gtcatcagtg gattgttaat cgtactcggt ttcttcagtc actttgtttt
     3421 agaaaacgta ggactttcag agtggtcttt gatcatcgcc tctgtctttg ggattacgcc
     3481 aattgccatt caagcgtttc aagcaatgaa agtcaaagtc atcagtattg atgtcttagt
     3541 cagtattgca gcgattggtg cgctttttat tcaaaattat gaagaatcgg ctattgtcac
     3601 gttcttattc ttattcggac actacttgga acaacgaaca ttgaaccaaa cgcgatctgc
     3661 tatcaaagaa ttgactgaaa tggcacccga aagcgccttg aaacaaatgg ataatggcga
     3721 atttgaagaa gtagaagtgg atgatataga tgaaggggat atcttgctcg ttaaaacggg
     3781 tgcgaaagtt ccagtagatg gcatagtcct tacgggtgaa gggcatatca atgaagctag
     3841 cattacaggt gaagctgttc cagtcaacaa gctagctgat gcagaagttt ttgcaggaac
     3901 cattttagaa aacggaacga ttcaaatcag agctgaccga gttggtgagg acaccacatt
     3961 tggaaaaatt attgaactcg tggaagaagc acaagattct aaatccgaag cggaacggtt
     4021 cattgatcgc ttttctaaat actacacacc agctgtcttg gttctggcaa ttgttgtatg
     4081 ggcgttcagt caaaatattg agttagcaat caccatcttg gttctaggtt gcccaggcgc
     4141 attagttatc ggagtgcctg tctcaaacgt tgccggtatt gggaatggtg ctcgtaacgg
     4201 tgttctttta aaagggagtg aagtcattca agacttcagc aatgtggata caattgtatt
     4261 tgataagaca ggtactttaa ctgtcggaaa cccaaccgtt gcagcgactg aactgtatga
     4321 aaaagatcct gctgaaacgc ttggctatct ggccagtgtg gaacgggaat cagatcatcc
     4381 attagcaaaa gcggtcttaa atcaaattgg agaaacaaac ttttatcctg ttgaaggaac
     4441 tgaagtcgtg aaaggcggcg gaatcgtttc aagtgtagct ggacatagag tggctgttgg
     4501 gaatgtggcc ttgatggaaa aagaaaatgt cactctcagc aaaaaggtgc aaaaagatat
     4561 caaacggttt gaacaacatg ggaactctct cgttttgaca gcggtcgacg gtgaattaaa
     4621 agtactgatg ggcattcgcg atcaagtccg tccgggtgtg aaagctaatt tgcaggaatt
     4681 aaaagacttg ggcgtgaaaa atctagtcgt tctttcagga gataaccaag gaaccgttga
     4741 tgtggtcgcc ggcgaacttg gtttgaccga agctcacggc cacatgttgc cggaagacaa
     4801 atcggcttat atcggaaaac ttcaacaacg tggtcaaatt gtagcctttg ttggagatgg
     4861 cgttaacgat agtccgtcct tagctttagc agacattgga atcgcaatgg gaagcggaac
     4921 ggacgtagcg attgaaacat ccgatgtcgt tttaatgaac tcgaacttca gcaacttgcc
     4981 tcacgcatta ggattagtaa aagccaccgc aaataatatg aaacaaaata tcgtgatttc
     5041 aattggagta gtgttggtct tgttgaccag cgtcttcttc agcgaatgga tgaatatgtc
     5101 tatcggaatg ttggttcatg aaggtagtat cttggtggta attttcaatg gcatgagatt
     5161 aatgaagtat aagttgaaag gtcgaaaaga acaaaaagaa gtatcagcac ctgcagagaa
     5221 agtaaaaata tcttaagaat aaattagaag aggctgagac aatagtccat ccttgaaatg
     5281 acaaaagcgg aaactcagat ttttaaatct gggattttcc gcttttttga gttcttgaaa
     5341 aaaaataaga gtatagagta tgttctttat aaaattaatt ttcgtgtaaa tgattaaaaa
     5401 att
//
LOCUS       DACDYJ010000122         5037 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDYJ010000122 DACDYJ010000000
VERSION     DACDYJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.124"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(81..1889)
                     /gene="pepF"
                     /locus_tag="IWM51_002691"
     CDS             complement(81..1889)
                     /gene="pepF"
                     /locus_tag="IWM51_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP9972035.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2065..3063)
                     /locus_tag="IWM51_002692"
     CDS             complement(2065..3063)
                     /locus_tag="IWM51_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP9972036.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3289..3942)
                     /locus_tag="IWM51_002693"
     CDS             complement(3289..3942)
                     /locus_tag="IWM51_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP9972037.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="IWM51_002694"
     CDS             complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="IWM51_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP9972038.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1494 a         1036 c          766 g         1741 t
ORIGIN      
        1 aaaaaaatac gtcgactccc ttacaagaga gccgacgtat ttttttcgtt tctattcgag
       61 aaaatcaaaa atagaaagcc ttataaacga tcgatcaatt cttctaattc atttagacgt
      121 tgttcaaaca tcgacattgc atcttcaata tatgcagcct gtgtcatatc aactcctgct
      181 tttttcatta cttccacagg ataatcgctg ttacctgctt ttaggtaagc caagtagttc
      241 tctagtgcct ctggttcctg gtttagtatt ttcttcgcca gtgctgaagc agcagaaaag
      301 ccagtcgagt attggaaaac atagtaatta taataaaaat gcggtatccg tgaccattcg
      361 aatttaattt ctgggtcttc ttcgactgct ggaccataat attttgcatt caatttacca
      421 taactatcac ttaggtattc actagtcaat ggcacacctt tttcatcttc cgtatgcatg
      481 aaatgctcga attcagcgaa ttgtgtctgg cggaaaactg ttcctttaaa cccatccaag
      541 taatggttga gtacataagc ccgtacacga gggtcttttt ctgtttccaa taaatactcc
      601 gttaggatat tttcattcgt tgtcgaagca atttctgcta aaaagatgga gtagtcgccg
      661 tacacataag gctggttcga acgagtgaaa taactatgaa cactatgtcc catttcgtgg
      721 acaagcgtaa atagctgatc cagcgtatca tgccaattca ataaaatata tggatttgtg
      781 tcatagcttc ccgaagaata agcaccgctt cgtttccctt tattttcgac aacatcgatc
      841 caacgttcag agaatgcttt ttctacgatg gccatgtatt cttcacccat tggtttcagt
      901 gcttctaaag ctttttcttt tgcttcttcg tacgtaaagg taattggagc ttcccctaag
      961 accggtgtat aaagatcata catgtgcagt ttttccactt ctaataaacg tttccgtaat
     1021 tccatgtatc gatgtaacaa aggcaaatgc ttgtttacca cgtctaccaa agtatcgtat
     1081 acactttcag gaatatgatt attgctcaaa gacgcttctc tggcagagct gtaattacgg
     1141 actttcgctt taaaattatg tccttttata tgtgtgccta aagtagaagc aaatgtattt
     1201 ctaaattgtt cgtaaacact gtacaacccc ttaaatgctg cttcgcgcac ccttcgatcc
     1261 gtgctttcta gcaactgacc atacacgcca tgagataatt ggactatttc accattttct
     1321 ccttcaatcg ttggaaaaac tagatctgca ttattcaaaa cagcaaatgt atcacttgaa
     1381 gcatcaaaga tttcacctgc tccagcaaga agagattcct gctcagcaga taagacatgg
     1441 gctcgattat ctactatttg ctggatataa tggcgataga cttccaattt tggttcttct
     1501 ttgaaatatt gccaaatctg gtcatctgac aattgcaata tttctggttc aaaccaggaa
     1561 acggcttcac taactttcga aaacagactg cttgctcttg cataaagcgc ttggtaatct
     1621 gtatttccag tgtcttgatc gtttttaagg tgcgcatata cataaatgac ttcagtttgg
     1681 cgataaaccc tcaatacgaa ttcaatcgca tttaaaaatt gagaagcgcc ttgatcaagt
     1741 gttcctttgt gtttttcaga ttgttttaac tcttctgata attccaaata tttctcatca
     1801 aactcttggt cgcttgaaaa gatcttggtc aagtcccaag ttaaattttc aggcaattct
     1861 tctcgttttg gcaactgctt tacttccatg aaatccactc cttttcttcc cttagctcta
     1921 tctatcaaaa tagatcatag tctaacatcc acatgtcaca gcatctaact tttctactca
     1981 ttcatgtgtc tgcttccatc aattttagca taactaaatc ttgttttaca tagaaaaccg
     2041 attcctagcc tatttttata cttactattt tttcaaagaa gacaagtgta tgcattcgtt
     2101 aaaaaactgc tgatagacca tctcttcatc aatcatagga aaaagccatt cccttttatg
     2161 ttcttttatt tggcaaagcc atgagttgaa atcaggaact ttctggtttt ccaaggcttc
     2221 ttcgtacaac attcgataaa gaaaaacctg ttcttgtaaa taaaagaaaa accgactttt
     2281 ttgatagatc cacgatgata gatgcaaaag atgtttatga cgcaagtagc attgttcttg
     2341 tatggatact gtattctttt gcttgtgaat aagttggcgt gtcagccatt tcttcccatc
     2401 ttcactcact ttttctattt ttttcatttg gttcatttta atttcgttac tagtgaatat
     2461 ttctttaagg tccctactag aaaaagtcca gcagctactg caaaaggaaa tatgccctga
     2521 tacagtctct gtaatatgat gatacaaata aagcttttgt ttcttgagat cagctttcca
     2581 gcaatgcact cctctttttc tattatagta gcagcaactt ttttctatca atgagaattg
     2641 cgattgtcca agaaaagaat taccaagtat ccaccatact tggtaactgt tattcaaata
     2701 attttgggtt ctttcaataa atctctggtg agacagtcta gaacattgga tttctattgc
     2761 tagattcgcc aacaaaatat ccggcctttg ccgcaacctc ggcaaatagg cttccaaaaa
     2821 tactggttct actgactgtc ccagccaatc catgaagcat tcctttaact gtaagtgctc
     2881 ttttgattct ctttcactaa agaccgaaca gttatctgat ttttgatggg caaaatgagc
     2941 acattttttc tttccctttt tcaaaatcac tctttttcga cagccacgac aataatattt
     3001 ctttcctttt tctgcatgtt tagcaaaaca gcacttatta tattcattca ttgcaagcag
     3061 catttttctc cctctgaact tatttacgtc taagaattta tttacacgaa aaaagagggc
     3121 gtgaaaaagc tattctgcat caagtaataa gaacagctaa acaaaaatag ttcccaatat
     3181 ttttcgtttg gttgggcttg ttaccgcaga tgcaagcttt tgaacacatt tatacgaaaa
     3241 aaagactgcg acacaatttt tgtcacagtc tcttactata atgctgattt atacaaaata
     3301 aaatttcgtt cgctcgatag cattacgcgc catcagacat tgtccatatt cattcaatgt
     3361 ttctggtgta cgatcgctct tttcagcaaa ctccaaaatt cgagcaatct gattctctac
     3421 atccgcttgg ttgagatttt ccatccagaa gtgaacgact aggtaatagc gctcttcgaa
     3481 taaatacaaa tcagtccaag cagactctag ttcgacatct gttgcaagtt ggatcacttg
     3541 ttcaaaatct tcaaaagcaa aagttgcttg tctttcctgt tcttctaggg cgcttccaga
     3601 taattcttcc tccggatcgc tgccaataat ctgctgacga agaaagtcac tcacatcttc
     3661 tgaatcaaca gtagaatctt ctgattgaga aatttcttca tccggaagat ttttactgat
     3721 aaacagttct aatccgtctc cttttggcaa aacttggaaa gtgacagctt cgcttcctcg
     3781 gaactcctct tcgatatcaa cttcttccaa aatactataa aagaaatttt cgatttcttt
     3841 gtgattgcca agtaaatcca aaaatgaaac gccccgttcc tctaaatctt catgaccaat
     3901 tagtacacgg atcgtatttt cattaatatg ttccatttcc attttgctac acctcacttt
     3961 gccacaatgt ttaatataga ttcattgtag cgaaatcagc ttaaatgtaa agaaaaaaaa
     4021 tctcgctgtt aagcgaattt aataaaacca gcggattttt caacctttca agctatctta
     4081 tttaactata acatttttgg cagacgaata ctatatagcc gacttttcag ctaattttcc
     4141 ctctctattt ttcgtgcaat catcatcttc tactaccttt ttatacatct aatcaagaat
     4201 actttttgta aaaacaaaaa aacttggaaa aactatcttt gtaatagtct tttccaagtt
     4261 tcgtattatc cttctataat ccagccatca attgtgcttg acgtaattct aattgtcgta
     4321 catcgcgcgg taggaaccga cgaatttcat cttcattaaa accaacttgc agacgtttat
     4381 catcaatcat gattggacgg cgaagcaatc caggattgtt ttgcacaagt tccaataatt
     4441 cttgtaacgg cagatcatct aaatccatgt tcagtttttg gaatactttg gaacgtgtcg
     4501 agatgatttc ttctgttccg tcttctgtca tttgtaaaat cgcttttaat tcagaactgt
     4561 tcaatggttc tgaaaaaata ttacgttcaa caaatggaat ctgatgttct tgcaaccacg
     4621 cacgagcctt tctgcaggaa gtacaacttg gggaagtata taatgttaac aacgtgcacc
     4681 actccttttc gtgataagaa tcactttttt gtaacagaag atcataataa tctcccatct
     4741 gtttctatta tacataatca acttttaaat agctaccttt ttttcataat ttttttaaaa
     4801 aaatgaaagt tatctaaggt taatatacag taaaaccagc attctcgctt tttttatcat
     4861 ttaaaaatta gagttttcaa atctaattca aattttctaa acatatgaaa cagcgtataa
     4921 aactgtacta ttattttatc tcaaaagcta aatgaaaccg atttcttaag attttttatg
     4981 ttttttattt ctttaatagt aatgttttct gtcattttaa ataatactgt tctttat
//
LOCUS       DACDYJ010000123         4987 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDYJ010000123 DACDYJ010000000
VERSION     DACDYJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.125"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            810..1484
                     /locus_tag="IWM51_002695"
     CDS             810..1484
                     /locus_tag="IWM51_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9972039.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            1481..1822
                     /locus_tag="IWM51_002696"
     CDS             1481..1822
                     /locus_tag="IWM51_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972040.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            1852..2211
                     /locus_tag="IWM51_002697"
     CDS             1852..2211
                     /locus_tag="IWM51_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP9972041.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            2414..2593
                     /locus_tag="IWM51_002698"
                     /pseudo
     CDS             2414..2593
                     /locus_tag="IWM51_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            2735..4816
                     /gene="cadA"
                     /locus_tag="IWM51_002699"
     CDS             2735..4816
                     /gene="cadA"
                     /locus_tag="IWM51_002699"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9972042.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
BASE COUNT         1680 a          800 c         1086 g         1421 t
ORIGIN      
        1 ggaagaggtt gtgcgcctgc cgttcagctc cgagtcacaa ggagtaattt caaaaaacag
       61 tttttcatgt ttgatgacta ttgtgacttg tgaccgagga gttggccgga gagcaccgtt
      121 tattctaaaa aagaactgcg aactctcgca gttctttttt taaaatacgg gataaaggaa
      181 gtttccagca gacaagagaa gaaagcaaaa gaaattgcta tttgattcac aaggttctat
      241 aacgaaatcg caatcaatca aacatttaca aagaaggtaa ttcaaatgga aatctgattt
      301 gatgatgctt ctgctgttga aataaagcag catctagcac ctgaaaacaa actaatagta
      361 acattcgaag atggagtagg tccgtatttt cagcatgcaa taatccatat gtaagttcaa
      421 ttcacccttg aaatcatcgg accagataaa gttatgacca atctatccca agcaatctag
      481 aagagatatt agtaaaggga catttactag aagtcttaga tgagcctata aatatatgtt
      541 ctgatggcgt catcagtggt ctgattgacg gaagcttagg tgtgatcgat ctctctggac
      601 caaacaacga aggaatcaag gggaatccgg cacgttaaga aaaaaatagc ataaaaaact
      661 agacacttga tgagcgttca ttcaattgtt tgaagggact aggctggtgt cattttttat
      721 acatatattt tctctttcgt cttgataggg cttatgggat aatataagga agaaaatcat
      781 cgttttattc aaaaaggagt ttttgacaaa tgaaaaaacg tatattattt atcggtgata
      841 gcattacaga cgcaggaaga gaccgagcag aagcaactga tctaggtaaa ggttatccac
      901 ttttgattgc cggaaaacta atctcccaat atccaacgat ggaatttgaa tgcatgaacc
      961 gtgggatagg aggagacaaa attgcagatc tgcaagtacg ctggcaagaa gattgtttag
     1021 ctttacaacc tgactatgta tcgattttga tcggaatcaa tgatacctgg cataatacta
     1081 gcgatcaggc tatattcgga actgagaaat ctgctcagca atttgaatcc gtttatcgaa
     1141 aattactaaa tgaacttcaa acagtgggaa tctgcaacat catcttgatg gagccgtttg
     1201 tacttcctat aaccgacgac cgtcatggct ggcgtaaaga cttagaccct aaaatccaaa
     1261 ttgttcgtaa acttgctaag gaatatggcg cattgctggt tcctttagat ggtatattca
     1321 atgctttagg gattgtttat ggtttttcga aatatacaag agaagatggg gtccacccaa
     1381 cgattggcgg acatgagatc attgcacaaa gctggataga tgcaattgcc gcagtcgatt
     1441 tttttagtgg agataaaaat gagcgaaaga aaagtgagaa atgatggaaa tacggtgggc
     1501 agtaaaagaa gatctacctc aaatcaatca gctgattact gatcatccag aatcattcgt
     1561 aaaagcaaaa acggcagctg acctaaatga taagaataaa ctattgctcg ttgctgtaac
     1621 aaaagaaaaa cggattatcg gatttgcgca agcggacgtg caacagtcag aagaacgatt
     1681 ggatcagcga ccatcaggaa aactaatcca acacacatta gtaacaaaag aaaatacgga
     1741 agaaatccaa aaaaaatttg cagaatattt gatactggaa gctaaaaaaa gaagaataaa
     1801 atcatttttt gcccagtttt aatcaaaaac agtaatagaa ggagaaatac aatgagttgt
     1861 ttaggaaatg tcatctggtt tatattcggc ggactaatcg gtggtcttag ctggttagca
     1921 gcggggatac tttggtgtat cacgatcata ggtatcccaa tcggtatgca atgttttaag
     1981 atcgcctccc tttcttttgc gccttttggc aaagaagtaa tttatcacca gtctggcatg
     2041 tcattgattg ttaatatcat ctggctgatc gtttcaggcc taccaattgc tctcgcacat
     2101 gtcgtcagcg gcatccttct atgtatcacg atcataggga taccttttgc caagcaatct
     2161 ttcaaacttg ctcgattggc gttgatgcca ttcggtgcag aaattgtata aaagagaata
     2221 ataaaggtta gataaagcat agagtcaggt gggatacatg tatttcacct gactctatgc
     2281 gttctcctgt ataagttctt ccatgtgact tttacgtctt ttatttatat ggggttactg
     2341 tttatggaaa gttgaaataa ttttcattaa agaactcttt ctatattttt gaaagcgtag
     2401 tggagcttcg aatatctggg aaggacattt tgcagggatt gaaaaaagat attgtcctat
     2461 cgaaaaatta atgaatttag acaaattgcc agcaacaggc ttcaaactta gttgctatcc
     2521 tgtcaagatc aagaaggcaa gtgcgggttg gataagagca gtggcaatga tcgaaaagaa
     2581 aaagaaagaa taagccaaaa catctgtatg gaagaaattt tccgatgcag atgtttttca
     2641 ttttttcttt cggatacctt gacatatgaa taaccgttca tttataatta gttatgaaga
     2701 gatattcata tgttgaggcg aggaggtcat taaaatgaaa agttacagat tagaaggttt
     2761 agattgtgcg aactgtgcaa tgaaaatcga aaaaggagtt cagaagatca atggagtcaa
     2821 ggaagcaacg gtaaacttca cttctggaaa actaacgatt gacgcagagg aagatcattt
     2881 ggctacgatt gaacaagaaa taaaaaaagt agtgaaggaa ttagaaccag atgtcaaagt
     2941 aacagaaata gataaggaaa aagtatctga acatggaaat gaaaaagaga gaaatacgct
     3001 gttccggatt ttattttccc ttgcaggtat cgctttattg ctattatttg attttaatga
     3061 accaattcgc ttgatagggt atttattgat ttatctgctg ataggatatg atgtagtgaa
     3121 gaaggcagtg atgaatatcg tcaaaggaaa aatctttgat gagaattttc ttatgtctgt
     3181 tgctacgatt ggcgctatgt tgatcggtga atatccagaa gccgtagcag tgatgctatt
     3241 ttatcaaatc ggcgagtatt tccaaggatt ggcagtcagt cattcaagaa aatcgattcg
     3301 cgaattgatg gcgatccgtc cagaaacagc tcacgtgcag acgacggaag gattgatgac
     3361 cgtgaaccct gaagacgtgc agatcggtca gtttgtactt gtgaagcctg gtgaacgtgt
     3421 accgttagat ggaacgatca tcgaaggaga atcattagtc gatacgtcag cacttacagg
     3481 agaatcagtc cctaaatctg tatatgttgg tgaaacagta ttaagtggat ttatcaataa
     3541 aaataaacca tttttagttc aagtagaaaa atcttatgaa aattctacta tcagcaaatt
     3601 attagaatta gtagaaaatg ccagcagtaa aaaagcacca gctgaaaatt tcattactaa
     3661 attcgcgcgc tattacacac cagtcgttgt aggattggcc gttttattag ctgttttacc
     3721 tccacttgtt gtttctggag cggcgttttc tgaatggatc tatcgagcat tgacattttt
     3781 agtgatctca tgtccatgtg cattagtgat ctccgtaccc cttagtttct ttggcggtat
     3841 cggaggagca agtaaaatcg gtgtattagt aaaaggttca aattacttgg agttgcttgc
     3901 acaaactgag acagtggtat tcgataaaac cggcacatta acgaaaggtg acttttcgat
     3961 ccagacgatc gatacaaaaa tcgatccaaa gatattcatg caatatgttg cgagtgcaga
     4021 acagttttct acacatccga ttgctcaatc tgttttagaa ggctacgcag gtccgctttt
     4081 accaacagct aatatccaag aatttgcagg tgaagggatc ttagctgaag ttgatggcaa
     4141 acaagtatta gcaggtaatc acaaattgat ggaaagattt gaaatctctt ttccttcaag
     4201 tcaagaaatc ggtacattgc tttatctagc gatcgatcaa agttacagtg gttatcttgt
     4261 tattgcagat accttgaaag aagatgccgt agacgcattg gttcaattaa aaaaggctgg
     4321 tgtgaagaac acagtcatgt tgacgggcga ttcgaaaaaa attgctgatc atatcgggaa
     4381 gcaagtaggc gtggacaaga tatatagcga attgcttcca gaagataaag tgcagcggtt
     4441 agaggaaatc ttacaaagaa acaataaaaa gaccgcattt gtcggagatg gaatcaacga
     4501 tgcaccggtg ctagcaagag cagatgtagg aatcgcaatg ggtggattgg gaagcgatgc
     4561 tgcaattgaa gcagcagatg tagtcatcat gaatgatcaa ccatcgaaga ttgcagaagc
     4621 aatccattta gcaaagaaaa cattgaaaat cgtcaaacag aacattgtct ttgctattgg
     4681 gataaaaatt ttggtacttg ccttaggtgc ttttggtttt gcatcaatgg gagatgcagt
     4741 atttgcagat gttggggtga cagtattagc tgttttgaat gccatgcgaa gcttgcatgt
     4801 aaaaaataaa cgttgatcaa tgaagaggac gaatgaacgt cctctttttt ttatatcctt
     4861 ttcttaatga aaaatgtaaa gaaaatagat aggattataa aataactaat gtttttaatt
     4921 ttgcagaaaa ttcaaaatag cttgaaattc aataaggtaa atgaaataaa agaaaataac
     4981 ttatttt
//
LOCUS       DACDYJ010000124         4961 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDYJ010000124 DACDYJ010000000
VERSION     DACDYJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.126"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..797)
                     /locus_tag="IWM51_002700"
     CDS             complement(<1..797)
                     /locus_tag="IWM51_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9972043.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVR"
     gene            complement(819..1412)
                     /locus_tag="IWM51_002701"
     CDS             complement(819..1412)
                     /locus_tag="IWM51_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972044.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(1413..1745)
                     /locus_tag="IWM51_002702"
     CDS             complement(1413..1745)
                     /locus_tag="IWM51_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972045.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(1823..2365)
                     /locus_tag="IWM51_002703"
     CDS             complement(1823..2365)
                     /locus_tag="IWM51_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972046.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            complement(2426..4804)
                     /locus_tag="IWM51_002704"
     CDS             complement(2426..4804)
                     /locus_tag="IWM51_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972047.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            complement(4801..4938)
                     /locus_tag="IWM51_002705"
     CDS             complement(4801..4938)
                     /locus_tag="IWM51_002705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972048.1"
                     /translation="MLIQFYEEQATDRLQEYVEKLIKNEEISEMIRKRLLLEAEKAVK
                     E"
BASE COUNT         1376 a         1011 c          754 g         1820 t
ORIGIN      
        1 aaacgtacac tatttttata cgtggcattc cagacaaatg ggccgcctgt catggtttcc
       61 ccatattgtg tgcctcctgg atcttttagc gtttggtggt ggaacgggaa tcctccgcca
      121 atgtttaacg atggaagtga ttgccctttg atatttccaa accattcagc ttgttcatcg
      181 aacatggtaa acatcgattg ccaatctcgt ttggttttat gcagataagt caccgcacga
      241 taatccatcc cttttagatt ttcgatcagt tcctgcgccc gttcatccac ttcttggatc
      301 gtatttccgt aaacatgtac tctcactttg acttgtttag aaatttcccc attagaagtc
      361 aattttcggg catattccgc tagttccagg tattcttccg cacttttatt tcggtcagtc
      421 gcttttcgtt cattatctga tcgatcattt aattcatcta atgcgcggtt gatttgatca
      481 atcacttttt gcttttcatc tgtgctaaca tctagtgtgg ccactgtatg aggattgttc
      541 atgagattgg ccaaccaaaa aagagtagga ttttttgtgt aatgataaac tgtcacacat
      601 cgtgaataac catttccata ggtcacatat tcttcatcaa aattcaaccc accttgtggc
      661 atcacttgtt ttaagaaatc taaatcataa ccttttgatt ttaaaatctc tatttcacga
      721 ttaccaagtt gttttaatgg atcaatcacc ttttgattac tttgcgttga aaaagctgtt
      781 tttatcacat ctagcatgaa tcatttcctc catctttttt attcgtcatt catgttgttc
      841 attcgataaa gaatcttaat caattctttt ttgtagagtg gttttaaccc taaaatgcca
      901 ctagatcgca ttaactgacg atttctagtt tgtaaatcct tgattgtctt tccttcaata
      961 acgaaaaaga agcttaagtc aggacgttct tgttctgccc gttttaattc ctggatctcc
     1021 tctactaatt tcttttcata ttttcttaat cgctgtagat ttcttcctag tgactgttcc
     1081 atacgatttt tatctaataa ctgccgcttt tgcacgattt gttccgttag atccgtctct
     1141 gtggtcaaac taactaactt aattgttggc acatacgtac gcatgacggt ggtcaactga
     1201 ttcattcgtt ctagtttctc ttgttcattt aatccctcca cgtcagtgga aacgacacga
     1261 taataacgct gaaaatgatc ttctctagta attaagactc cctcttctgt acaggattta
     1321 tatggaaata agtccaagat agagcgctct tctggttttc caaactgcaa aatggatttc
     1381 ttatcaactg aattttgtcc tttgactcgc atttattcat cctccttata cgtaccatga
     1441 taatttcggt taggatctaa cgcatgatag tggttcttat ccgtaaagaa aatagattcg
     1501 ataatcgcaa aaatttggcg ttgaccaggg ttattatagg gtcgccaaac aatcactaac
     1561 gctatcgcaa gccataatcc ataattcaag agtgcaaaaa agagtggtaa gtggacaaga
     1621 ttattgacta ttttcgccca aaaaaagcag ccaccaagcg ttaatagatc taaaaagaaa
     1681 aaggcgaaaa acttccattc cccactaaat cttggaggat ttttcatatg aatgtattta
     1741 tccataatat aaccataccc cttatatttt gtatttttag aaataaaaaa cactgtgatt
     1801 tctcacagcg tttttctaaa tattacaact ctaattcatt tcctaaatca cgagaacgat
     1861 tttttcctcg tcgtaattga taattcaatt gacgattttc ttcttttaat tcaacaattt
     1921 tttgttccag atttcttttc ttttgaatta aatcataatt ttgtgcgcgt aattcttcat
     1981 tttccaaatg aactcgttgc atttcatcgt ttaatcgatt tacattctcc aatgattctt
     2041 gtatcgtttc ttgcctaaaa ttaagtgagc caagttgacg atctttttgt tgtctttgct
     2101 ctttcaattc tgacgatatt tgctcaacag aatgaaatat ttccgttaat tttactgtga
     2161 atgtgggcat agcatctttc aaataagtga atccttctga aaggtgtaca ataatgcttt
     2221 cggtatgtgt tttttgacca tctaaaatag actgatgatc tttttgtaaa ctatccgtat
     2281 ctttaaatcc tttaatagtc accacaaata tcgttaaaaa tgctcccagt gcagttataa
     2341 acgtagctat taaaatatcc tccacaattt tctcctttcc attttaaact tgttttataa
     2401 agaaaaaggc ccttaaatag gccttttatg gtttgatatc cttcaatagt tgttctttca
     2461 attcattgtt cctgttttta gtttctcttg tcacatttat ttttgtcggt gcagtaaatg
     2521 ctacgtttgc tggtctgtat ccattatgaa ctttattgat tggcttaggt gcagcttctt
     2581 ttgtcacaat attttgaacg gacgcattct ctgtttcacc actttcagct aaattgtaag
     2641 aaacagttgt cagatttcca ttttcaggca aacgacttga tttcatttga acagtacctg
     2701 caggtgattg atgaacaaag ctttgatcat tctctttgaa ttgattcaaa cgactatctt
     2761 tcattggtaa attttgtgtc ttaggcaaac caattttcgt tgatttttca gttccaactt
     2821 cgatgccatt atcatttggc agatcgatgg aatcctcatc cgtttgtact tcttgatttc
     2881 gttttggtaa atctgaaatg cgtttttcgt tttcagaatt aatgttagat ttataacgat
     2941 attgttctgg atcggttttt ggtaactgac catgttgtcc ttcttctgaa tctttttctc
     3001 cgatcgaatt ttcgttagaa tgtaactcat cttggtgatc tttggctgaa ggaatcgctg
     3061 caccttcttg attttctttt tcacttgttg tatttgcctt cactgctgca ttgacttgtc
     3121 cttcaccata cgcatctttt agtccttctt tgactgctga agctttttct ttttgttttt
     3181 ctagtgcttc ggatggattc ttcacagctt cagcaaattc tttagctctc gtgccgtcta
     3241 atttctcttc taaggtgtct ttggcttttt ctgtaagatc ttcaactgtt ccttttaacc
     3301 cacgttcatt gacgtaacca agaccttctg cggctttaga aacaccattt ttcgctttcc
     3361 cgaacatttc ggaaactgtc ttcttactat taggtacagt tgtagcttta tcgtctgatt
     3421 gttcttcccc gttatcatgg gtaatatctt ttcgatggcc aaatccattc ttacgaatat
     3481 catttaaatg gcttaaacca ctcttaccag cacttgcaag gtttttagct ccgctaccca
     3541 catttttcgc aatatttcct aaggcaaaca tgctaagaag agaattgctg ccgtttttca
     3601 gactcgtatc aacaccgaaa atcttcgtaa ccgattgaga gcctttgaat agcgcaatag
     3661 ttagcacaat taaagcaaca acatacaaga atccattgat ctttcctacc gcatgcaaat
     3721 caccgatacc tgaaagcatt aatgtataga ttcgtaattc taaaatcgta aacgccaata
     3781 acaagaagga ttgagaaata tcttctatcg cacttttcat ccgttgtcct gtctcaaagt
     3841 ccgttgacgc ggcaattact aaatagaatt ttttaaatac tagctcaata atgcaagtaa
     3901 cgattgtaaa caaaataaat aaataggcta ccgacaaaga agattcccca gccaaaataa
     3961 tccctggact actaatgtat cgacgatagc ctgacgtata cacatatttt gctaaacctt
     4021 catcactaat ttgaacaggg atttctttcc cattatcgtc taactctagt cgatactgta
     4081 atgcctttaa ccgatctttt tgtactgtac taatatcact tgattcagac attttcttag
     4141 cttgatcagg tgtaataatc ccattgatat tcgctgtttt gaaatttttc gctgttaaag
     4201 catttctttt atctgttggt aaggattcta ctttattcgg gtcagtcgct agtaatgtac
     4261 tgacatcaat cacattattt tgaataattt gaaaagcagg tgaacccgtt ttatctgctt
     4321 tatttgctgc agtgacgtct ccataaaagt ttttactgat atttaaccct tcatcgatta
     4381 aagaactacc accaaaaatc aagatcatgg ccataaataa attaaccaag atatttttga
     4441 ctttcggtgg gtgtttactc ataaaaaatt tcaagcctaa ataaattaat gtgacgacac
     4501 aaaaaagagc caatacctta ctaatcaaac tttgaaatag ttcattaaca ccagcggcgt
     4561 tcaataagtc ttttaaatca aagctttgat aaattgtttt ttctaataaa gtattgagat
     4621 tatataaccc tttgatcaat ccccataaaa aagtattaca aatccaactg aatacatctt
     4681 gatactgcag ataattacca taatcttgta agactttcgt tacggttttg ggatctgcat
     4741 ccccttctcc gaataaacct gaccaccagt cggctatgcc agagaaaatt cctccaatga
     4801 tcattctttc accgcctttt cagcttccaa taacagccgt ttcctaatca tttcagaaat
     4861 ttcttcattt ttgatcaact tttcaacata ttcttgaaga cgatccgttg cttgttcttc
     4921 gtaaaattga attaacaatt ttttagccgt ctcattctca a
//
LOCUS       DACDYJ010000125         4913 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDYJ010000125 DACDYJ010000000
VERSION     DACDYJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.127"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(45..752)
                     /locus_tag="IWM51_002706"
                     /pseudo
     CDS             complement(45..752)
                     /locus_tag="IWM51_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
     gene            complement(931..1338)
                     /locus_tag="IWM51_002707"
     CDS             complement(931..1338)
                     /locus_tag="IWM51_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP9972049.1"
                     /translation="MNKTLRLMKPWDYLIITAVIMASFVPYLLFDTNNDENNNRESTD
                     VIAVVKIDGEVVDKFNLSQVNESFEKTYYPQKGKYNIIEVAPGKIRVKEDNSPDQIAV
                     RTGWISRPGEMSICLPHKLIVEILGVEENPLIY"
     gene            1610..2977
                     /locus_tag="IWM51_002708"
     CDS             1610..2977
                     /locus_tag="IWM51_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9972050.1"
                     /translation="MKKTFISTSVIVFSLFFSNGLLAKAQTIEEKIKEKDDKIEAIVK
                     NKESAENYLTDLDNKITQLEKEYKKVLVEKNTSEKKLTEINQKITALEERIRLRNKLI
                     EEQARNAQVNQKSDSIISVMVNAESLSDAVTAAIGMTKLISASNEIMQAQIDDKENLD
                     ELKKEAEKKVTDAEKETRSLEEKENDLIEAKLTQSIKINEISASLATEQSEKAKFENQ
                     KQEAEKRREEELKAIAEEKRKEAEAAAILKAQEAEAASIIKDQEKDVSQLADTNDIGI
                     GQENTDTQKEEQIPDETSEQDSNSVNQNEYQDNNSSSDNTQIQSPSSSGWGAPVANII
                     VTSPFGGRADPTGFSGSFHDGIDMGGTSSTPIMASRSGTVVQASFDGSAGNYIIIDHG
                     DGYYSYYLHLSNYIATPGQSVSAGQTIGTMGTTGNSTGVHLHFGIATSSNWSGFVDPA
                     PFLGI"
     gene            2980..3668
                     /locus_tag="IWM51_002709"
                     /pseudo
     CDS             2980..3668
                     /locus_tag="IWM51_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337548.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
     gene            complement(4206..4502)
                     /locus_tag="IWM51_002710"
     CDS             complement(4206..4502)
                     /locus_tag="IWM51_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972051.1"
                     /translation="MANNLHYVTNLESRQFDPCWHQIDAQTKELLLGWEENQVVRDFS
                     LNFMMIDYYNLATSMQRFPNYGMDIDSFKQKSFSGEIQNEESNNLVIPILYEPG"
     gene            complement(4584..4850)
                     /locus_tag="IWM51_002711"
     CDS             complement(4584..4850)
                     /locus_tag="IWM51_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9972052.1"
                     /translation="MTSTYASFDKDNLYYDFPSVSGFSSMNNKKYLLMLTRLGYSTSN
                     VRAKRNGGTVITDILLSNQYQVIPNQEIQHQATSGVKIIESEVS"
BASE COUNT         1705 a          829 c          796 g         1583 t
ORIGIN      
        1 tcatcgtcat tcctcctgct atctttttct attattctac cttatttgat agtagattta
       61 aaactttgca acagaacctc gcgaacaata taatttagtt tcccaatatc aaataaaaaa
      121 gctgagatag cacccaaatc tttcgaaaaa agtcctccta agcagttgag ttcaatcata
      181 ccatcacgga gtttaaaata gtcctcccat ttacctaagc gaattttcga gcccgtaaga
      241 taattatatc catttacaat agctgtgtta aaaggaggga aaatggtagg ttctagaaaa
      301 tataaaatgt ttgcgacagc tggaccaagc cctctgattt tcttttctgc tagaatattt
      361 acagcaaata acattttaat ttcttgatta gctttaagaa tttgatcaag acaaagtgaa
      421 aaagctaact gattttccct attttcataa atatccggaa ttctcaattt aggtttccac
      481 ataaatgcat gcttggctcc tttaaaaatt tctgtttgtt ctgcaattac attcattatg
      541 tcctctaggg ctgaattttt aaaatctgtt gggaacgttc cgttattgac atgtttgata
      601 agtacttgta cgtatttcct aactttttta aacgctttca aacgtttttc acttttgata
      661 aaccaagttt gatacagtaa attttcgcgt aatttatatt gattaaacac atctgttaaa
      721 taagtattat ccactgtaat gacacctacc atccctatta atattttata aactcgttag
      781 ctctaatatg gctttctata tgcaaaattc cgtaaccacc ttaaatgaag aataatgtaa
      841 acaggtttta gcgattttag ttaacggtct ttccttagat tactccgtcc atgtctctat
      901 aataggattc gtggtactta ttagttcata ctaataaatt aaaggattct cctctactcc
      961 taaaatttca acaatcaatt tatgaggcag acatatactc atttcccctg gtctactaat
     1021 ccagcctgta cgaacagcaa tttgatcagg actattatct tcttttaccc tgatttttcc
     1081 aggagctact tcaataatat tgtatttccc tttttgaggg taatatgttt tctcaaaaga
     1141 ctcattaact tgtgataagt taaatttgtc tacaacttct ccatctattt taacaactgc
     1201 aattacatct gtactttccc tattattatt ttcatcatta ttagtatcaa atagtaaata
     1261 tggtacgaat gatgccataa tgaccgcagt aataattaaa tagtcccaag gtttcatgag
     1321 tctcaatgtt ttattcacta aattcgttct cctgtattaa cttttgatat catactttac
     1381 agttgtgtga attttatgta ggtatgtata tgctccataa aatctccata agttttagtt
     1441 attttgaagc tgtctattaa attgaaatat agcatatgga tcctattact ttttgtatag
     1501 taattccaat ttcaattcct agtagaacct gtagcaacga taaaatagag aggagaatat
     1561 tatgtaaaat gtttcttcac aaataaaata agggaagtga aaaatcaatt tgaaaaaaac
     1621 atttatatct acctctgtca ttgtgttttc gctatttttt agtaatggtc tcttagctaa
     1681 agcacagact atagaggaaa aaatcaaaga aaaagatgat aaaattgaag cgattgtaaa
     1741 aaataaagaa tcagctgaaa attatcttac tgatttagat aacaaaataa ctcagctaga
     1801 aaaagaatat aaaaaagtgc tagtggaaaa aaacacatct gagaagaaac ttaccgagat
     1861 taatcagaaa attactgctc ttgaggaaag aatacgcctt aggaataaac ttatcgaaga
     1921 gcaggctagg aatgcacaag taaatcaaaa aagtgactct ataatatctg tcatggtaaa
     1981 tgccgaatca ttatcagatg cagtcacagc agccattggt atgactaagc ttatttctgc
     2041 tagtaatgag attatgcagg cccagataga tgataaagaa aatttagatg aattaaaaaa
     2101 agaagctgag aaaaaagtta cagatgcgga aaaagaaact cgatctctcg aagagaaaga
     2161 aaatgacctt atagaagcca aattaactca aagtataaaa atcaatgaga tatccgcttc
     2221 tttggcgaca gagcaatccg aaaaagcaaa atttgaaaat caaaaacaag aagctgaaaa
     2281 aagaagggag gaggagctta aagctatagc agaggaaaag agaaaagaag cagaagctgc
     2341 ggctatttta aaagctcaag aggcagaagc cgcatctatt ataaaagatc aggaaaaaga
     2401 tgtatcacaa ctagccgata ccaatgacat aggaataggt caagaaaata ctgatacaca
     2461 gaaagaagaa cagataccag atgaaacttc ggagcaagat tcgaactcag ttaatcaaaa
     2521 tgagtaccaa gacaacaata gttcttccga taatactcag attcaaagtc ctagttcttc
     2581 tggttggggc gcacccgtag ccaacattat cgtcacaagt ccatttgggg gaagagctga
     2641 tcctactgga ttttctggat cttttcacga tggtatagat atgggtggaa cttcttcaac
     2701 gcctatcatg gcatcaagat caggaactgt agtgcaggcg tcatttgatg gatccgcagg
     2761 taattacatt attattgatc acggagatgg ttactattca tattaccttc acctaagtaa
     2821 ttatattgct acaccaggcc aatcagtgag tgctggacag accatcggta ccatgggaac
     2881 tacaggaaat tcaaccggag ttcatttgca ttttggaata gcgactagct caaattggtc
     2941 tggattcgta gatcctgctc catttctagg aatctaaaaa tgatgaaaaa aatcaagcat
     3001 ctttctttaa atttacttct aattagcctt tttcttatag gcttagtttt aatatttaat
     3061 ttccaaattc gatcttactt actgaataaa caagtccaca atatgacata gcaaattatt
     3121 cacagaaaga tttggaagac aatctgaaaa ggaaaggaaa ctttgatttt gattctgttg
     3181 aatctatatc aaatgagtta gtattaaaag cacaattcaa gaataatgat ttgcctataa
     3241 taggtggtat cgctatacac aatgtaaaat tatctttacc aatatacaac ggattggaca
     3301 acacctcgct cctttctggg gctggaacaa tgaaaccaga tcagcagatg ggaaaacgaa
     3361 attacgcttt agcaagtcat agtgctaaag ataaaactat tttatttttc ccattagagt
     3421 ttatttctct aaaagaaaaa atatatgtaa cagacttaaa gaatatttat atttacaaag
     3481 tgtcctttaa agaaaaagtg gatccatcaa gagtcgaagt cattgatgat attcctaata
     3541 aaaatatgat cactcttgtt acatgcggag atttggctgc tgtaacgaga ttgattgtac
     3601 aaggtacact gatcgataca gttgaattta agaatgctaa tcaatatatc cattctttat
     3661 ttagctaata taaaaagtct tatcaatcct gacagattac gagtagtact ttttattaaa
     3721 gaaagaaggt gctttctaaa ttcaaattgg tttatggtca taataaatta aatagggaaa
     3781 cgaacgcgat tataagttcg tttctctatt ttgtatgagt gtctttaatc taattgaaaa
     3841 gagccctttt tgatccaacc tcaagaaaat tttgaaaaaa tcttactatg gagctaacta
     3901 gtaacgcaca ctatttaatc atttaggaat ttgcttatat cccccttgtc attctatagt
     3961 ttctagggaa gatttagatt gtgattttcc taaggaatat cctctgtttt cttgatataa
     4021 tccagaaaaa gtaaaaataa aaagcaccca gttgcaatac tagagattac caagcctacc
     4081 aagaatcctg gtgcgaaaaa ttctccagca attttcatat tactatcttt tggtaagtta
     4141 gacaattgaa tcaaatcgtc aaaagctact ttgtaaggat actcttcccc gtccacagta
     4201 aaaacctaac ctggttcata gagaataggt ataacaagat tattgctttc ctcattttga
     4261 atttctccag aaaagctttt ctgtttgaat gaatctatgt ccataccata attcgggaac
     4321 cgttgcattg atgtcgccaa attatagtaa tcaatcatca taaaatttaa tgagaaatca
     4381 cgaactactt gattttcttc ccaacctagc aacaattctt tcgtttgagc atcaatttga
     4441 tgccagcagg gatcaaattg tcgactttcc aaattggtca catagtgcaa attattggcc
     4501 aagttgacgg gaaaataaac ataaagtaac tcatcttttg ttacttctag aacgtcaaat
     4561 gaaaaagaag caatatctga ggtttaactc acttcgcttt ctataatctt tacaccgcta
     4621 gtcgcttgat gctggatttc ttgattagga attacttggt attggttgct tagcaaaata
     4681 tccgtaataa ctgtaccacc attccgttta gcacggacgt tacttgttga gtaccctaat
     4741 ctcgttaaca ttagtaaata ttttttattg ttcattgaac taaatcctga tactgagggg
     4801 aaatcatagt agagattgtc tttatcaaag gatgcataag ttgaagtcac tttttttagt
     4861 ggttcccaat attcttgata tacttcaaat tctgttagag tttggttgat tgt
//
LOCUS       DACDYJ010000126         4887 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDYJ010000126 DACDYJ010000000
VERSION     DACDYJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.128"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            85..972
                     /locus_tag="IWM51_002712"
     CDS             85..972
                     /locus_tag="IWM51_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9972053.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="IWM51_002713"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="IWM51_002713"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP9972054.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="IWM51_002714"
     CDS             2274..2720
                     /locus_tag="IWM51_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9972055.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="IWM51_002715"
     CDS             2993..4345
                     /locus_tag="IWM51_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP9972056.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="IWM51_002716"
     CDS             4516..4803
                     /locus_tag="IWM51_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972057.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1681 a          805 c         1022 g         1379 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttt
//
LOCUS       DACDYJ010000127         4833 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDYJ010000127 DACDYJ010000000
VERSION     DACDYJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.129"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            149..1576
                     /locus_tag="IWM51_002717"
     CDS             149..1576
                     /locus_tag="IWM51_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9972058.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1668..2033)
                     /locus_tag="IWM51_002718"
     CDS             complement(1668..2033)
                     /locus_tag="IWM51_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9972059.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(2030..2395)
                     /locus_tag="IWM51_002719"
     CDS             complement(2030..2395)
                     /locus_tag="IWM51_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9972060.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2587..2835)
                     /locus_tag="IWM51_002720"
     CDS             complement(2587..2835)
                     /locus_tag="IWM51_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972061.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2834..2962
                     /locus_tag="IWM51_002721"
     CDS             2834..2962
                     /locus_tag="IWM51_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP9972062.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3019..4713
                     /locus_tag="IWM51_002722"
     CDS             3019..4713
                     /locus_tag="IWM51_002722"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP9972063.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
BASE COUNT         1592 a          918 c          849 g         1474 t
ORIGIN      
        1 attatctgtt ttccaaattt tataacataa aaaacctttt ttaacggcat ttatatgctt
       61 tttcagaaat taactgatga tttcaaaaag aaaacatgtc ataatatttc ccatattgtg
      121 caaaaaaata caaggcggag gctctactat gattgaagaa tatattgaga aaaatatttt
      181 aagacagctg tttttatgtg ggcaattcta tgtaaataaa gaagtcaatc tagaaaagct
      241 ctccaatctc ctgcatgttt gcaaaaccac tttactcaat gatatcaata acataaaaaa
      301 agaatttgaa gaacagatcg cttatacaca tcgggaaaag gaccgttaca ctttatattt
      361 ttcagaacat atacctcgat gcaagatcat gcagcaattg tctcaaaatt cgttgttttt
      421 aaaaacttgt ctgctttatt tggaagaaga tgaacctgat tatcttcaat taactgaatg
      481 tgaattcatc tctgtcagca aagcttattc attaaaaaaa caagtattag cttactttaa
      541 tgactgcggg atcgaaatcg accgctactc ccctcgattc actgaaatgg aaagacgtct
      601 gctgcttctt aatgtgtcgt atcgtttagg cggttttaat tcttgggagc tacctgaatc
      661 gttttttgaa cgtgccgatc gtttcattga atccgtaacg gaaaatagtg gcagatttta
      721 tgataaagaa aataaagaaa tcttatcgat tggttttgct atcagtttcc tacgccagca
      781 agtgtgtgct gtgacaatag attcaaaatt tatcgaagaa atcaaaaaac gtcctattta
      841 caattatgtc gaatcagcat gggaaaacac tgatttccaa acctattata aaaaagaaga
      901 attcgccttt attttgacac tattcaatct ttgtaattat ggtttccatt cttatcagtt
      961 gattgcggaa gactttcaac agctacacca agtgtttatt gacaatactc ccgaaataaa
     1021 agaactagtt gctacctttg aatctcactt caatcaagaa ttattcggaa accaaccgtt
     1081 cgaacgtgcg ctgattcatc tgatgcgttc ggcttgggat aactatcaat tattcatgcc
     1141 agaaaaattt tatcttttaa atgaggaaca gacaaattta tataaagaag tccagacaat
     1201 tttttccagc tggtccagcc agttacctta tgatctacgg ttgaatccta attgcatgcg
     1261 ggcttttgtg atcgaattga gcggaattct ccgtctgaca aaagaacatc tgactatcta
     1321 tatcgtcaca aattcggatg ttcattatct gatttatcgg gaagccttgg aagctgtaac
     1381 tactttcgat tttcaagttg caccaacaat ttattcaagt atatctgata taaagaagta
     1441 tgcgcagcag tcctcaaacc gggtattatg tgaacgtaca ttatatacac cagatgcggt
     1501 ccaatatgaa aatattattc ctatttcgat caatacgatc gatcgagcta tcatttcagc
     1561 tgtgcaaaac aaataagggg ctgtgacaaa aatcatgtca cagcctctta tttgttgaag
     1621 atgcccattt ctgtaacaat ctcttgatac acgtcaggct cacaacctca cttattttgg
     1681 acatctaggg gtccgatctc tttatttaaa aaataagaaa tgatggttcg gataactacc
     1741 aacgccgcta gttggaaaat atcctgcaag gtaggtttga taatcgattc gatgatccca
     1801 gctgaaacaa gaatttccaa acttagaagt atatagcctc ccaacatctt tttaatagca
     1861 ttgttctcgc ttactcggtc gctttgcact tttgtatgga caaataaata tttcaaaaaa
     1921 tccttgattg atagacaaac accccaaacc aataaacaga tagcaaagat atcaagtccc
     1981 agtacaagtt tttcgaaata tggaagaaga aactgcatca actctgtcgt catcctacct
     2041 ctcctcttct ttgtttttct ttgtcttcta tttcctgatt caagaaataa gaaatgactg
     2101 ttcgaatgat aataataagt gcaagcttca aaatatctgt ccaagtcggc ttgataactg
     2161 actcgatgat atcagcaacg actagtactt ccaagcttaa caaaatataa cttgctagca
     2221 gttttcttgt atctgcatta cgagtattac gttctgcata gtttttcttg acatgagacc
     2281 aatcaaaaag tttgaataga acaagaacga tcccgacaat taagataccg atagaacaaa
     2341 tatttaaaat agcaatgatc cattctagaa attcaattaa atttgagcga aacatatacg
     2401 ctacctactt tcttttaatt tgttcctatt cttttattgt aaaagaaagt taccttgttc
     2461 gtctatttaa aagttgttct aatgataacg gttcgcttaa atttttcttt ttcactctcc
     2521 catttttctc cctccatcca taaatcgtgg aagtatccaa aacaacgtag caagttaaaa
     2581 taaggcttag tccgtcatca gcttttcaaa gaagcactgt ctcggtgctt tttttgtacc
     2641 caaatttcta aaaatagaac catacacctc ctcaaatctt ttcttcttac ctaccctatt
     2701 tttatttaca ggaaaatcag acacttcttc ttttttgtca aaatttcctt tttcttctcg
     2761 tcctatcatt tggcattcta ataaaaacga actatccttg gatatagaaa gagaaaacaa
     2821 ggaggcgttt ttcatgagta aaaaattaat caaagtcggc atcggtttag gtttgcttgc
     2881 ccttggcgct gcatatctag gaaaaaagac aggactattc gaagatgaca gtcatttata
     2941 tgatgaattc gaatcaatct agcatcctat ttagttgaat cactacggac atacttggaa
     3001 gacattggag gaatcacaat gagaaaaagt aaagcaataa tgattggtgc cggtattgcc
     3061 aatatggcag cggctgttta tttgattcaa gaaggcaaat ggaaagggga tcagatcacc
     3121 ttttattcat tggatgatca cggttcaaat gatggtgcac cgaccgacac tgtagtggat
     3181 gaatattgga ataaaaacca tccattagaa aaccaaaaag gctatattgc tcgaggcggt
     3241 cgcatgctta attaccgtac ctatgtagat ttgatggatc tgttaagtcg aataccttct
     3301 gttactgagc cagggatgac tgctgaagaa gatacgagag attttgatgc aaaacaccga
     3361 acctttgaca aagcacgttt gatggaaggt ggcaaaggaa tcattgatgg cggaaaactg
     3421 ggattcaata ataaagaccg tattcttctg actaaactga ttgctatgcc tgattcagaa
     3481 gaagaaaaac tagacaatat cactatcgaa gaatatttca aagatgatcc acactttttt
     3541 gaaacaaact tctggtttat gtgggaaaca acatttgctt tcagaacacg tagttctgct
     3601 caagaattgc gtcgctatat gcatcaaatg atttatgaat tcactcaaat cgaacatcta
     3661 gtgggcgtca atcgaacacg ttacaatcaa tacgaaagta ttatgcttcc tttgatcgag
     3721 tacttgaaag aacaaggatg caaaatcatt ttgaaccgcc gtgtcactga ctgggaattc
     3781 aaagaaacac cgatgcaaga tgaaatcact gtgactggtt tgaaaattac caatacactg
     3841 actcaagaag aagaatacgt tgtggtcgat gaagatacag cagttatttt cactaatggg
     3901 tccattacag attcagctac tttaggagat tacgaaacac cagctgtaga aaatatggat
     3961 tatggtgcag cttctagtct ctggaaaaaa gccagtgaac gtttctacaa cttgggaaat
     4021 cctgacaaat tctttgctga tcgagatgca agcgaatggg tcagctttac cctgacgacg
     4081 aaaaaccatt tgttgttgaa tgaaatcacc cgtatcacga ctcaagaacc aggaaatgcc
     4141 ttgaattcat ttatttcaac cacaccgatc acgccgttag gccaaaaaga tgtcaatatg
     4201 tctattgtcg tgcatcatca accgcatttt acgtctcaga aaccaaatga aacagttatt
     4261 tggggttatt tcctttatcc aagacgacgt ggcgaatttg tggataaaca gtatattaaa
     4321 atgaatggaa aagaaatgct agaagaactg attggacaac tttcaaaagt agatcctgga
     4381 ccagtgaata ttcgtgaaaa agaaacagaa attttcgaca gcgtaataaa taatatccct
     4441 gtctatatgc cttatgcttc cgcgttgttc aataatcgtg ccaaaagcga tcgaccaaaa
     4501 gtcattccta aacattcgac caatctggcc tttacaggag aatttgtcga gcagccttat
     4561 cagatgatct ttactgaaca aagtgctgta cgttctggag aaatcgctgc tttccatttt
     4621 gcgggtattc ctatgtcccg tcttgtcaaa acaccgcgtt acgacaaaga tattcctact
     4681 ctagctcgtg ccgcaaaaaa aatgtttgaa taatcaagag gttgtgaaaa agctgccctg
     4741 catcaagtaa taagaacaac caggctaaaa cagcttctta tgtttttgat ttggttggac
     4801 ttattaccgc agatgcaagc ttttgaacac cgt
//
LOCUS       DACDYJ010000128         4777 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDYJ010000128 DACDYJ010000000
VERSION     DACDYJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.130"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(183..1145)
                     /locus_tag="IWM51_002723"
     CDS             complement(183..1145)
                     /locus_tag="IWM51_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9972064.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1147..2586)
                     /locus_tag="IWM51_002724"
     CDS             complement(1147..2586)
                     /locus_tag="IWM51_002724"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP9972065.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2771..4717
                     /locus_tag="IWM51_002725"
     CDS             2771..4717
                     /locus_tag="IWM51_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP9972066.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1523 a          815 c          787 g         1652 t
ORIGIN      
        1 aacagttgtg taaaaatatc attttagagc atgaaaaaag gagtggattt ctccactcca
       61 aaaaagtcta acttttgggg gtcactacaa gttgacaact cctttttgtt tacaaatcaa
      121 ttggatatct acaaattgta ttttctaacg aattttatag acattaaaaa attaatttaa
      181 cgttaaatac ttcctccaga aagtattgag attggtaaaa tataatcctt atttgtcttt
      241 tcgcctttaa tttttttcag aagaatatca acaataagac ttgcaatttc atcaattggt
      301 tgtcttattg ttgtcaattg tgggaaaaat ttctcaataa aagaggtacc atcgtatcca
      361 ataattttta aatcttctgg gatattaagt ttaagttgat gagcaatttt catagtgaga
      421 atggctgtta aatcatcaga aacaaaaata ccatctggtt tgtgatttat aatagttgac
      481 tttatttcca tttcacgacg gatagtagac agattattag gaattttgaa aatttttcca
      541 tttggatttt gaaaagaaaa acctagtgca cgtaattctg tgggagaatc cgtgttatca
      601 tttcctgtaa tcataatggt attttgacaa ccattttttt gtaaagtctt agctgcaagt
      661 tttccccctt caaagttatc tgaagaaaca atggaaacat taggagctaa atttctatca
      721 aaagcaacaa taggtgctct cacacgctca taatcagcaa ttccaagatt atggcttgat
      781 gagattatgc catcaacttg gttggcttca agcatttcaa tgtattctct ctctttagca
      841 ggatcatttt gactattgca aataatcgtt ttatatccat gtttaaataa ctctatttcg
      901 agatactcaa tgagttcaga gtaaaagata ttactaatat taggaaaaat aagaccaata
      961 agctgaggtg actttccttg aagtccacgt gctaaattat ttggtttata accaagagtt
     1021 ttcatagctt catgaacttt ttgttttgtt ttttctgata aatatccctt attattaatg
     1081 acgcgtgaaa cggttgttgg gctaactcca gctaattcag cgacatcagt taattttgct
     1141 accattttaa tattttaatt caaagtattt tccagtaggt tcgcctgatt tgatttgaat
     1201 accattttgg tgattttcag ggaatacacg actagtaaac actttttctc ctttattaat
     1261 aaaaatctca atgattgagt tatctacaaa aatgttagca gttgtttctt tagtatcaat
     1321 tgagcattca cgaatcgtac caaagtcagt ggcatattga atgcctgctt tgctgcgatc
     1381 taaaataatt tttccttctt tggtatctac aatcaagcta agtccatttc ctttactatc
     1441 agagaaaaga agtaactccg atttctgatt tggaggaaaa gttagttcaa gctcataagt
     1501 attatttgtt tctgcttttg ctgtaaattg ttcagaacta gcacgtaatg atgtaatagc
     1561 ttctactgga tattggtaaa gtttaccatc ttttattgaa agttctttta caagactcat
     1621 tgctccctga taatcatact tatcagttgg ataatcgata tcaggtagtc caatccaact
     1681 gacaatcaga gcacgaccat caggagtatt gaatccttga gtcgcatacg cttcaaatcc
     1741 ataatcaaga ttttgaattt ctgaagcgcc cacaagtttt actttttcta tgtcaagcga
     1801 ttgacagatc ttaaaagtat tcggatagat attcccataa tttagctcag atttatccaa
     1861 cccttgagga caataaatga gaacaggttt tttgtctaca aatactagat ttggacactc
     1921 aatcatgtat tctgaaccag tgccgccaaa atcaaggttg ccaacttcct cccatgtttc
     1981 cacgttattg tcatccgctt tgtaaagttt gataaagcct gatttttcta aattttgtgc
     2041 tccaacaatc gcatacaatt gtccttcata attaaacatt tgtggatcac gaaaatgatc
     2101 agttacatca gcaggtttat gaataagaac attctcacct ttcgtaatgt tgtagtcttt
     2161 atccatccat gctccgattt gaagtgtatc acgttcccaa ttttcatttc gaacgtttcc
     2221 tgtatagagt aagaatagat tcccctctat ttcatatgca gaaccggaat aagcgccgtg
     2281 gctatctcgt tttgtgtctg gaagaagttc tgctcctgtt acatgaaaat gaactaaatc
     2341 atctgattca ttgtgtaccc attctttcaa cccatgagca gctccaaatg gccaattttg
     2401 ataaaaaagc gtatattttc cgttaaaaaa agaaaaacca ttaggatcgt ttaacaatcc
     2461 tgttttaggt tcgatatgat agctagcacg ccaaggtgat cgttttacat tatccttaat
     2521 tttatccatc tcatctttag tccaatcaga ataagcacga tatcgtattt ctcgaggtaa
     2581 gttcatattt tactccttca atttatcttt ttacaactag tatggtaaac gttttccgtt
     2641 gaaaaatcaa tcatttcatt taaaaaaata aactatatga caaacgtttg acatattttt
     2701 aagatatgct aaaataaatt tgcatttcag tgaaccgttt tcacgtaaaa ataaaaaagg
     2761 agtgattcga atgaattaca gtaaagtagc ttccgacgta atacaagcgg tcggaaaaga
     2821 taaccttatt gcagctgcac actgcgctac acgtcttcgt ctcgtactaa aagatgacac
     2881 gaaagtgaat caaaaagctt tagacgaaaa tccagatgtg aaaagcacat ttaaaattga
     2941 tggacaatat caagtgatta tcggtgcagg tgatgttaat ttcgtttatg acgaacttat
     3001 caaaaaaact ggactttcgg aaatttcaac agatgattta aaacaaattg ctagtaaaga
     3061 taaaaaattc aaccccatta tggcattaat taaattatta tcagatattt ttgttccaat
     3121 tatacctgcc cttgttgcag gtggtttatt aatggcatta caaaactttt taacagctgc
     3181 tggacttttt ggaccaaaat caattgaaga aatgtatcct gcgattgaag gtatttcctc
     3241 aatgatccaa ctgatgtccg ctgcaccatt catgttctta ccaattttag taggtatttc
     3301 agctgctaaa cgttttggag caaaccaatt tcttggtgct gcaattggta tgatcatgac
     3361 aacaccagat cttggtggct caactgaata ttggaatatt tttggttatc atgtttcaca
     3421 aacaaactat gcttatcaag ttattccagt tttagcagct gtttggatat tatctatatt
     3481 agaaaaattc tttcataaaa aattgccatc atctattgac tttacattca cgccattatt
     3541 atcagtaatg attactggat ttttgacttt cacagttatt ggccctgtaa tgttattgtt
     3601 gtctaatgga attacagaca ttattgtttg gctatataac actactggat ttattggaat
     3661 gggtattttt ggaggaacat attcacttat agtcatgact ggtttacatc aatcatttcc
     3721 agctattgaa actcaattac tttcagcgtg gacaaatggt atcggacatg gagactttat
     3781 ctttgtagtt gcatctatgg caaacgttgc tcaaggtgct gcaacatttg ccatttggtt
     3841 cttaactaaa aattctaaga caaaaagtct tgcttcctct gcgggtgtct cagcacttct
     3901 tggaattaca gagcctgcct tgttcggagt taacttaaaa taccgctttc cattcttttg
     3961 tgctttaatt ggttcaggaa ttgctacagc agtagcagga ttacttaaag tagttgctgt
     4021 ttcacttggt tcagcaggtt tcttgggatt tctttcaatt aatgcaacat ctattccatt
     4081 ttacgtattg tgtgaattga ttagctttgt agttgctttt gcaattactt acttctacgg
     4141 taaaacaaaa gctgctggta tctttaaagc agaagaaatt gctgaacaaa caccagttaa
     4201 ttcttcaaaa gtagcaacaa cagatccaat atctactgat gttgaatcaa cacaaacaaa
     4261 agtttcatca gaaacaattg ttagtcccat ttctggtaaa gtgattagtc tttcttcagt
     4321 aaatgatcca gtcttttcat cagaagctat gggaaaaggg attgccatta aaccaaatgg
     4381 aaacacggtc cattctccag tagatggtac tattcaactc acgtttgaaa ctggacatgc
     4441 ttatggtatt aaatcaaata atggtgctga agttctaatt catattggta ttgatactgt
     4501 atcaatggga ggaaaaggat ttacacaaaa agttactact aatcaaaaag ttagaaaagg
     4561 cgatattctt ggagcatttg accgttcaat gattgagaaa gaaggtcttg atgatacaac
     4621 aatgattatt gtgaccaata cttcggatta ttcagaagta aatatagtag ctgaaaatac
     4681 agtcactgag ggtactgatt tacttgaaat caaataaata tatttccctg agtaaagatg
     4741 gaattttctt tccaaaatca aataatatag attaatc
//
LOCUS       DACDYJ010000129         4714 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDYJ010000129 DACDYJ010000000
VERSION     DACDYJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.131"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(213..443)
                     /locus_tag="IWM51_002726"
     CDS             complement(213..443)
                     /locus_tag="IWM51_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP9972067.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(673..1491)
                     /locus_tag="IWM51_002727"
     CDS             complement(673..1491)
                     /locus_tag="IWM51_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9972068.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1652..2341
                     /locus_tag="IWM51_002728"
     CDS             1652..2341
                     /locus_tag="IWM51_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9972069.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2355..3857
                     /locus_tag="IWM51_002729"
     CDS             2355..3857
                     /locus_tag="IWM51_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9972070.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3870..4346
                     /locus_tag="IWM51_002730"
     CDS             3870..4346
                     /locus_tag="IWM51_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9972071.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4443..4553)
                     /locus_tag="IWM51_002731"
                     /pseudo
     CDS             complement(4443..4553)
                     /locus_tag="IWM51_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1590 a          673 c          830 g         1621 t
ORIGIN      
        1 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
       61 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      121 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      181 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      241 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      301 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      361 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      421 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      481 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      541 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      601 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      661 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
      721 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
      781 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
      841 taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat
      901 ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga
      961 taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa
     1021 atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc
     1081 aagactagta tttccactag atcccactcc aaatatatat atgttttttg attgagtgat
     1141 cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc
     1201 ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt
     1261 ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat
     1321 tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact
     1381 catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc
     1441 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
     1501 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
     1561 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttgttatcca
     1621 aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt tagatcttat
     1681 ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt acaaagagga
     1741 agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg
     1801 tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg
     1861 aattattaaa agagattatc caccagaatc tccattcatt actgccacta tgagagaagt
     1921 agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg
     1981 acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca
     2041 gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat
     2101 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
     2161 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
     2221 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
     2281 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
     2341 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
     2401 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
     2461 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
     2521 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
     2581 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc
     2641 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
     2701 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
     2761 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
     2821 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
     2881 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
     2941 cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt
     3001 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
     3061 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     3121 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag
     3181 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     3241 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     3301 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     3361 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     3421 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     3481 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     3541 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     3601 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     3661 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga
     3721 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     3781 gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat
     3841 taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag
     3901 aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg
     3961 tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt
     4021 atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa
     4081 aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag
     4141 gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg
     4201 ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt
     4261 ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt gctcatggga
     4321 attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa aataagctaa
     4381 ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt ctgttcattt
     4441 ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt aaagttataa
     4501 aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt caaatataaa
     4561 atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg tttactcaac
     4621 ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga accttagcac
     4681 tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DACDYJ010000130         4707 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDYJ010000130 DACDYJ010000000
VERSION     DACDYJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.132"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..391)
                     /locus_tag="IWM51_002732"
                     /pseudo
     CDS             complement(1..391)
                     /locus_tag="IWM51_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            467..622
                     /locus_tag="IWM51_002733"
                     /pseudo
     CDS             467..622
                     /locus_tag="IWM51_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            641..1918
                     /locus_tag="IWM51_002734"
     CDS             641..1918
                     /locus_tag="IWM51_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9972072.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1928..2584
                     /locus_tag="IWM51_002735"
     CDS             1928..2584
                     /locus_tag="IWM51_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9972073.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2584..3960
                     /locus_tag="IWM51_002736"
     CDS             2584..3960
                     /locus_tag="IWM51_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9972074.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(4246..>4707)
                     /locus_tag="IWM51_002737"
     CDS             complement(4246..>4707)
                     /locus_tag="IWM51_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAP9972075.1"
                     /translation="DLNKSIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTML
                     VFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCDVGDISF
                     SIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
BASE COUNT         1770 a          575 c          830 g         1532 t
ORIGIN      
        1 tcattttcga tcggtttcgc ttgataatag taggtctgac gagaaatatt tagaattttg
       61 cacatcgctg atatggaata tttatgcttg ttggcatcaa ttacttgtct tttcgtccga
      121 atatcagcgc cgcttgcttt aaaatatcat tctccatttc gagttgctta tttttctttc
      181 ttagtgcgat caattctgct tgttctggtg ttaagttgtc tctttctttg aatgagcccg
      241 ttgattgtgc ttgcttcatc catttatcga aagaagaagg cgtaagctca tactctcgaa
      301 taatttctgc gcgaggctta ccagcgagat agagatcgac gatttgttgt ttgaattctt
      361 ttgaataggt tcttcgttga cgtcttggca tgaaaaatcc tccagtgtgt tttttattat
      421 tctacacacc ttattttttc tgtctagtct agtgtagccg attcaggata aggataagtg
      481 tattaattgt ggaaaatgta aaaaagtttg ccaaatgaat aaagattcag ttgagaattg
      541 caatcatctg gagtgtatca gatgtggacg atgtaaaaat gcttgtccag tggatgcaat
      601 tagttatgga gtaagaaaat agagaggagt agttaggaaa gtgataaaaa atgctatagc
      661 atatattaca agaaagaaaa atagaacttt aataattttt atcatattaa ctatagttct
      721 ttcttgcttg tattcttgtt taacaataat gaaatcaagc aatgaaatag aaaaggcttt
      781 atatgaaagt tctaattctt caatatcaat aacaaaaaaa gatggtaaat attttaatgt
      841 taatcaattt aaagatattg aaaaattaaa agaaattgaa gaaaaaataa ttcaatatga
      901 tggattagca aaattaaaag atgctaaagt agttagcgga gaacaaagaa taaatagaga
      961 agatttatct gacgaattta agaatgttgt ttcactcgaa gctacaaata atactaaaag
     1021 aaatatttta tttagtagtg gagtatttac aattaaagaa ggtaaaaata tagaagaaaa
     1081 tgataagaat tcaattattg ttcatgaaga atttgctaaa caaaacaatc taaaattagg
     1141 tgatgaagtt gatcttgaat tactagatat tgaaaaaagt ggaaaaataa aaagtcataa
     1201 atttaaaatt atagggatct tttctggtaa aaaacaggaa acatatacag gattatcttc
     1261 tgattttagt gaaaatatgg tgtttgtaga ttattcaaca agccaagaga tattaaataa
     1321 ttcagaaaat aatgaaattg caaataaaat tttaatgtat tctggtagtg cagaatctac
     1381 agacttagcc ttaaacaaat tgaaagagct taaaattgat gagtcaaagt attttgttga
     1441 aaaagattct aatgcgtttg aagagtcttt agagtcagtg agtggaataa aatatataat
     1501 aaaagtaatg acttattcta ttatgttagg tgggatggtt gttctttcat taatcttgat
     1561 tctatggtta agggaaagaa tttatgaaat aggtatattc ttatctattg gaacatctaa
     1621 gatacaaatt ataatgcaat ttatattcga gttaatattc atatcgatac caagtataat
     1681 atcttcctta tttttaggaa atgtattact aaaagtaatt gtagatggat tcattaactc
     1741 agaggattca atgatttccg gtggaagttt aataaataat agtagtttta tgtcaaatat
     1801 aacaacactt ggacaaagtt atttaatatt aataagtatt attgttttat cagttgtatt
     1861 tgcttcttca ttaatgttaa tcaagaagcc taaagaaatc ttatcaaaaa taagttagga
     1921 gaaaataatg gatatattag aaataaagaa tgtagcatat agttatgcaa attctaaaga
     1981 aaaagttttg tcaggagtaa atcaaaaatt tgaacttggg aagttttatg cgatagtagg
     2041 aaagtcagga acaggaaaat ctacacttct ttccttactt gctggacttg ataaacctca
     2101 aacaggaaaa atattgttta agaatgaaga tatacaaaat aaaggatata gtaatcatag
     2161 aaaaaataat atatctttag tatttcaaaa ctataattta atagattatt tatcaccaat
     2221 tgaaaatatt agattagtaa ataaatcagc agatgaaagt atcttgtttg aactaggttt
     2281 agataaaaaa caaataaaaa gaaatgttat gaaattatct ggtggacagc aacaaagagt
     2341 agctattgct agagcattgg tatcagatgc tccaataata ctagctgatg agcctactgg
     2401 taatctagac agtgttactg ctggagaaat aattaatata ttaaagacat tagctaaaga
     2461 tagaaataaa tgtgtaatag ttgtaacaca tagtaaggaa gtagcagatt ctgcggatat
     2521 cattttagaa ctaagtggta aaaagttgaa aaaagtaaat aaaatgaatt tggaggttga
     2581 ataatgataa aaaatgcatt tgcttatgta actagaaaaa gtttaaaatc attaattatc
     2641 atattagtta ttttatctat gtcaacttta agtctcatta gtttatctat taaagatgct
     2701 acggacagag cttcaaaaga aacatttgct aatataacaa atagtttttc tatggagata
     2761 aatagacaag taaatccagg aacacctaga ggtgggggaa atgtaaaagg tgaagatatt
     2821 aaaaagatat ctcaaacaga tagtatagac tcctatgtaa aaagaataaa cagtgttgca
     2881 gatttagttg attatgatat tattgaaact caagagactc ttgcgaatca atcacctgaa
     2941 agagcaaaga attttaaaag aacagttatg ttaactgggg ttaacgactc aacaaaagaa
     3001 acaaaatttg tatcagaagc atataaatta gtagaaggaa agcatttaga aaatgaagat
     3061 aaaaataaaa tcttaatgca taaagattta gctgaaaaaa ataaccttaa agttggagat
     3121 aagataaaaa taaaatctaa tttatttgat gctgataatg agaaaggtgc agatgaaaca
     3181 gtagaagtag aaattaaagg tctattcgat gggcataata gcggtgtagt aagtgcagca
     3241 caagaactat acgaaaatac attaatcact gatattcata cagctgctaa agtttatggt
     3301 aataccgaag atacagctgt ataccaagat gcaacattct ttgtaaaagg tgataagaat
     3361 cttgatagtg taataaaaga tcttgggaaa ttagatataa attggagaga atataatttg
     3421 attaaaagtt catcaaatta ccctgcatta caacaatcta tatcaggtat ttactcaatt
     3481 tcaaataaat tattttttgg ctcattaata tttgcaggag ttgtagtttc attattatta
     3541 ttcttatgga tgaatgctag aaagaaagaa atagcagtat tactatcatt aggtatatca
     3601 aaattagaaa tttttggtca attcctaatt gagatggtat ttatatcaat ccccgcattc
     3661 gttggttcct atttcttagc tcaatataca gctgacaagc ttgggaataa tatattgaat
     3721 aaggttactg gtgatatagc taaacaaata gctaaacaat ctgcatctag ccaattaggt
     3781 ggtggggcag aagctgaagg atttaacaag acattatcaa gtttagatat caatgtatta
     3841 cctaaattca tgatttatgt agttatattt atgagtttag tacttcttgt atcattgatt
     3901 atttcttcat tcaatatatt aagaaggaat ccaaaagaat tattaattga caataattaa
     3961 aattttcggt gctttttagc accaaaaatg agagtgtaat ataaaccgtg taagtaacca
     4021 tcaatcctag gcgggtggtt gcttcacggt ttttattttt ctaaaataag atcaagaggt
     4081 gattacacga tggctagaaa gaaaagaaat cctgatgctg agaagttagc tgaatccatt
     4141 ttgaatactt atcaacccga atctgtagag gatatgcaag atgttttaaa agatgtgttt
     4201 ggtcccttat ttgaaaaaat gcttcaagga gaattgaata accatttagg gtatgacgcc
     4261 cattctaaag agccaaagaa acatgacaat cgacgaaacg gttatggcaa taagacgctt
     4321 aaaacgagct ttggtaaagt aaacattgat gttcccagag accgggaggc ttcttttgaa
     4381 cctgagttga ttcctaatag aaaacgagat gtctccgaca tcgcagactg tagacaaaag
     4441 ctgttccaaa ctcgtaactc gagcttggaa cagcttttta tctacttcgt ttcgaatttt
     4501 tgaacaaaaa aataaaggct ccttgccttt ccatagccag gtggctaatt tctttaaatt
     4561 cattgcagca aacactagca tcgtatgcgt cgcgactttt tccaagcctc gatacttggt
     4621 ccaacgcatc ccatgctttt cttttgcatc cgcaaatatt cgttcaatcg tttgtttccg
     4681 ttttttgtaa attgatttat tcagatc
//
LOCUS       DACDYJ010000131         4669 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDYJ010000131 DACDYJ010000000
VERSION     DACDYJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.133"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..263
                     /locus_tag="IWM51_002738"
     CDS             <1..263
                     /locus_tag="IWM51_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972076.1"
                     /translation="AYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFT
                     NGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            361..1734
                     /gene="tet(L)"
                     /locus_tag="IWM51_002739"
     CDS             361..1734
                     /gene="tet(L)"
                     /locus_tag="IWM51_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP9972077.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            2298..3560
                     /locus_tag="IWM51_002740"
     CDS             2298..3560
                     /locus_tag="IWM51_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAP9972078.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3805..>4669
                     /locus_tag="IWM51_002741"
     CDS             3805..>4669
                     /locus_tag="IWM51_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP9972079.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKKLNL"
BASE COUNT         1618 a          638 c          956 g         1457 t
ORIGIN      
        1 gagcatacaa cgatgctcct aaatattgtg cgaacatcgt agacactcaa ttgaaaaata
       61 atgaggtcat tcttagtgga gaaatccctg ctcggtgtat tcaagaatat cgtagtgatt
      121 taactttctt tacaaatgga cgtagtgttt gtttaacaga gttaaaaggg taccatgtta
      181 ctaccggtga acctgtttgc cagccccgtc gtccaaatag tcggatagat aaagtacgat
      241 atatgttcaa taaaataact tagtgtattt tatgttgtta tataaatatg gtttcttgtt
      301 aaataagatg aaatattttt taataaagat ttgaattaaa gtgtaaagga ggaattaatt
      361 atgaagtgta atgaatgtaa tagggttaaa tacaaccaaa ttttaatttg gctttgcgtt
      421 ttatcttttt ttagcgtatt aaatgaaatg gttttgaacg tctcattacc tgatattgca
      481 aatgatttta ataaaccacc tgcgagtaca aactgggtga acacagcctt tatgttaacc
      541 ttttccattg gaacagctgt atatggaaag ctatctgatc aattaggcat caaaaggtta
      601 ctcctatttg gaattataat aaattgtttc gggtcggtaa ttgggtttgt tggccattct
      661 ttcttttcct tacttattat ggctcgtttt attcaagggg ctggtgcagc tgcatttcca
      721 gcactcgtaa tggttgtagt tgcgcgctat attccaaagg aaaatagggg taaagcattt
      781 ggtcttattg gatcgatagt agccatggga gaaggagtcg gtccagcgat tggtggaatg
      841 atagcccatt atattcattg gtcctatctt ctactcattc ctatgataac aattatcact
      901 gttccgtttc ttatgaaatt attaaagaaa gaagtaagga taaaaggtca ttttgatatc
      961 aaaggaatta tactaatgtc tgtaggcatt gtatttttta tgttgtttac aacatcatat
     1021 agcatttctt ttcttatcgt tagcgtgctg tcattcctga tatttgtaaa acatatcagg
     1081 aaagtaacag atccttttgt tgatcccgga ttagggaaaa atataccttt tatgattgga
     1141 gttctttgtg ggggaattat atttggaaca gtagcagggt ttgtctctat ggttccttat
     1201 atgatgaaag atgttcacca gctaagtact gccgaaatcg gaagtgtaat tattttccct
     1261 ggaacaatga gtgtcattat tttcggctac attggtggga tacttgttga tagaagaggt
     1321 cctttatacg tgttaaacat cggagttaca tttctttctg ttagcttttt aactgcttcc
     1381 tttcttttag aaacaacatc atggttcatg acaattataa tcgtatttgt tttaggtggg
     1441 ctttcgttca ccaaaacagt tatatcaaca attgtttcaa gtagcttgaa acagcaggaa
     1501 gctggtgctg gaatgagttt gcttaacttt accagctttt tatcagaggg aacaggtatt
     1561 gcaattgtag gtggtttatt atccataccc ttacttgatc aaaggttgtt acctatggaa
     1621 gttgatcagt caacttatct gtatagtaat ttgttattac ttttttcagg aatcattgtc
     1681 attagttggc tggttacctt gaatgtatat aaacattctc aaagggattt ctaaatcgtt
     1741 aagggatcaa ctttgggaga gagttcaaaa ttgatccttt ttttataaca ggaattcaaa
     1801 tctttttgtt ccattaaagg gcgcgattgc tgaataaaag atacgagaga cctctcttgt
     1861 atctttttta ttttgagtgg ttttgtccgt tacactagaa aaccgaaaga caataaaaat
     1921 tttattcttg ctgagtctgg ctttcggtaa gctagacaaa acggacaaaa taaaaattgg
     1981 caagggttta aaggtggaga ttttttgagt gatcttctca aaaaatacta cctgtccctt
     2041 gctgattttt aaacgagcac gagagcaaaa cccccctttg ctgaggtggc agagggcagg
     2101 tttttttgtt tcttttttct cgtaaaaaaa agaaaggtct taaaggtttt atggttttgg
     2161 tcggcactgc cgacagcctc gcagagcaca cactttatga atataaagta tagtgtgtta
     2221 tactttactt ggaagtggtt gccggaaaga gcgaaaatgc ctcacatttg tgccacctaa
     2281 aaaggagcga tttacatatg agttatgcag tttgtagaat gcaaaaagtg aaatcagctg
     2341 gactaaaagg catgcaattt cataatcaaa gagagcgaaa aagtagaacg aatgatgata
     2401 ttgaccatga gcgaacacgt gaaaattatg atttgaaaaa tgataaaaat attgattaca
     2461 acgaacgtgt caaagaaatt attgaatcac aaaaaacagg tacaagaaaa acgaggaaag
     2521 atgctgttct tgtaaatgag ttgctagtaa catctgaccg agattttttt gagcaactgg
     2581 atccaggaga acaaaaacga ttttttgagg aaagttataa attattttcc gaacgatatg
     2641 gcaagcaaaa tattgcttat gcaacagttc ataatgatga gcaaacccct cacatgcatt
     2701 taggtgttgt gcctatgcgt gatggaaaac tgcaaggaaa aaatgtgttt aatcgtcaag
     2761 aactgttatg gctacaagat aaattccccg agcatatgaa aaaacagggt tttgagttga
     2821 agcgtggtga acgtggctct gaccgtaaac atattgagac agctaaattt aaaaaacaaa
     2881 ctttggaaaa agagattgat tttctagaaa aaaatttagc agttaaaaaa gatgaatgga
     2941 ctgcttatag cgataaagtt aaatcagatt tagaagtacc agcgaaacga cacatgaaaa
     3001 gtgttgaagt gccaacgggt gaaaagtcca tgtttggttt gggaaaagaa ataatgaaaa
     3061 cagaaaagaa accaaccaaa aatgttgtta tatcggagcg tgattataaa aacttagtga
     3121 ctgctgcgag agataacgat aggttaaaac agcatgttag aaatctcatg agtactgata
     3181 tggcgagaga atataaaaaa ttaagtaaag aacatgggca agttaaagaa aaatatagtg
     3241 gtcttgtaga gcgatttaat gaaaatgtaa atgattataa tgagttgctt gaagaaaaca
     3301 agtctttaaa gtctaaaata agcgatttaa agcgtgatgt gagtttaatc tatgaaagca
     3361 ctaaggaatt ccttaaggaa cgtacagacg gcttaaaagc ctttaaaaac gtttttaagg
     3421 ggtttgtaga caaggtaaag gataaaacag cacaattcca agaaaaacac gatttagaac
     3481 ctaaaaagaa cgaatttgaa ctaactcata accgagaggt aaaaaaagaa cgaagtcgag
     3541 atcagggaat gagtttataa aataaaaaaa gcacctgaaa aggtgtcttt ttttgatggt
     3601 tttgaacttg ttctttctta tcttgataca tatagaaata acgtcatttt tattttagtt
     3661 gctgaaaggt gcgttgaagt gttggtatgt atgtgtttta aagtattgaa aacccttaaa
     3721 attggttgca cagaaaaacc ccatctgtta aagttataag tgaccaaaca aataactaaa
     3781 tagatggggg tttcttttaa tattatgtgt cctaatagta gcatttattc agatgaaaaa
     3841 tcaagggttt tagtggacaa gacaaaaagt ggaaaagtga gaccatggag agaaaagaaa
     3901 atcgctaatg ttgattactt tgaacttctg catattcttg aatttaaaaa ggctgaaaga
     3961 gtaaaagatt gtgctgaaat attagagtat aaacaaaatc gtgaaacagg cgaaagaaag
     4021 ttgtatcgag tgtggttttg taaatccagg ctttgtccaa tgtgcaactg gaggagagca
     4081 atgaaacatg gcattcagtc acaaaaggtt gttgctgaag ttattaaaca aaagccaaca
     4141 gttcgttggt tgtttctcac attaacagtt aaaaatgttt atgatggcga agaattaaat
     4201 aagagtttgt cagatatggc tcaaggattt cgccgaatga tgcaatataa aaaaattaat
     4261 aaaaatcttg ttggttttat gcgtgcaacg gaagtgacaa taaataataa agataattct
     4321 tataatcagc acatgcatgt attggtatgt gtggaaccaa cttattttaa gaatacagaa
     4381 aactacgtga atcaaaaaca atggattcaa ttttggaaaa aggcaatgaa attagactat
     4441 gatccaaatg taaaagttca aatgattcga ccgaaaaata aatataaatc ggatatacaa
     4501 tcggcaattg acgaaactgc aaaatatcct gtaaaggata cggattttat gaccgatgat
     4561 gaagaaaaga atttgaaacg tttgtctgat ttggaggaag gtttacaccg taaaaggtta
     4621 atctcctatg gtggtttgtt aaaagaaata cataaaaaat taaaccttg
//
LOCUS       DACDYJ010000132         4614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDYJ010000132 DACDYJ010000000
VERSION     DACDYJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.134"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..797)
                     /locus_tag="IWM51_002742"
     CDS             complement(<1..797)
                     /locus_tag="IWM51_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9972080.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVR"
     gene            complement(960..1553)
                     /locus_tag="IWM51_002743"
     CDS             complement(960..1553)
                     /locus_tag="IWM51_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972081.1"
                     /translation="MRVKGQNSVDRKSILQFGKPEERSILDLFPYKSCTEEGVLITKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(1554..1886)
                     /locus_tag="IWM51_002744"
     CDS             complement(1554..1886)
                     /locus_tag="IWM51_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972082.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTIGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(1938..4457)
                     /locus_tag="IWM51_002745"
     CDS             complement(1938..4457)
                     /locus_tag="IWM51_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972083.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAAEMADNDKSGLSDLQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLSKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKS"
     gene            complement(4454..4591)
                     /locus_tag="IWM51_002746"
     CDS             complement(4454..4591)
                     /locus_tag="IWM51_002746"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972084.1"
                     /translation="MLIQFYEEQAADRLQEYVEKLIKNKEISEMIGKRLLLESEKVVK
                     E"
BASE COUNT         1242 a          965 c          724 g         1683 t
ORIGIN      
        1 aaccgcacac tatttttata cgtggcattc caaacaaacg ggccgcctgt catggtttcc
       61 ccatattgtg tgcctcctgg atcttttagc gtttggtggt ggaacgggaa tcctccgcca
      121 atgtttaacg atggaagtga ttgccctttg atatttccaa accattcagc ttgttcatcg
      181 aacatggtga acatcgattg ccagtctctt ttcgttttgt gcagataggt cactgcacga
      241 taatccatcc ctttgagatt ttcgatcagt tcctgtgctc gttcatctac ttcttgaatc
      301 gtgtttccgt aaacatgtac tctcactttg acttgtttag aaatttcccc attagaagtc
      361 aattttcggg catattccgc tagttccagg tattcttccg cgcttttatt tcggtcagtc
      421 gcttttcgtt cattatctga ccgatcattt aattcatcta aggcacgatt gatttgatca
      481 atcacttttt gcttttcatc tgtgctaaca tctagtgttg ccactgtatg aggattgttc
      541 atgagattgg ccaaccaaaa aagcgtagga ttttttgtgt agtgataaac tgtcacacat
      601 cgtgaataac catttccata ggtcacatac tcttcatcga aatttaatcc accttgtggc
      661 atcacttgtt ttaaaaagtc taaatcatac cctttggatt ttaaaatttc tatttcccga
      721 tttcctagtt gctttaatgg atcaagtacc ttttgatcat tttgtggtga aaaagctgtt
      781 ttaattgctt ctaacatgta cagtcacctc ttcttgtttg attctttact tggttaaaag
      841 attttccttt tcccaactta cccaggctta aagtgtgagg aaattttcta gtggtctctt
      901 ctttcggaac atctttttct aaccatatca acggatattt cattctctct tccccattcc
      961 tactcatcat tcatattatt cattcgataa agaatcttga tcaattcttt tttgtagagt
     1021 ggttttaacc ctaaaatgcc actagatcgc attaactgac ggtttctggt ttgcaaatcc
     1081 ttgattgtct ttccttcaat gacgaaaaag aagcttaaat cagggcgttc ctgttctgct
     1141 cgtttcaatt cctggatttc ctctactaat ttcttttcat attttcttaa tcgctgtaga
     1201 tttcttccta aggactgttc catacgattt ttatctaata actgccgctt ttgcacgatt
     1261 tgctccgtta gatccgtttc tgtggtcaaa ctaactaact taatcgttgg cacatacgta
     1321 cgcatgacag tggtcaactg attcattcgt tccagtttct cttgttcatt taatccttcc
     1381 acatctgtag aaactacacg ataataacgt tgaaaatgat cttctttagt aattaagact
     1441 ccctcttctg tacaggattt ataaggaaat aagtccaaga tagagcgctc ttctggtttt
     1501 ccaaactgca aaatggattt tctgtcaact gaattttgac ctttgactcg catttattca
     1561 tcctccttat acgtaccatg ataatttcgg ttaggatcta acgcatgata atggttcttg
     1621 tccgtaaaaa aaatagactc gataatcgca aaaatctggc gttgacctgg gttattatag
     1681 ggtcgccaaa caatcactaa agctatcgca atccataagc cataattcaa gagtgcaaaa
     1741 aagagtggta agtggacaag attattgact attttcgccc aaaaaaagca accaccaatc
     1801 gttaatagat ctaaaaagaa aaaggcgaaa aacttccatt ccccactaaa tcttggtgga
     1861 tttttcatgt taatatattt atccataaat gatttctcct tttcatacat agaaaaaagg
     1921 cctaactcgt aggcctttta tgatttgata tccttcaata attgttcttt caattcattg
     1981 ttcctgtttt tagtttctct tgttacattt atttttgttg gtgcagtaaa tgccacattt
     2041 gctggcttgt aacctgtatg aacgttttga ataggttttg gtgcagcttc tcttgtcaca
     2101 acattttgaa cggacgcatt ccctgtctca ccactttcag ataaattgta agaaacagtt
     2161 gtcagatttc cattttcagg caaacgactt ggtttcattt gaacagtacc tgcaggtgat
     2221 tgatgaacaa aactttgatc attatccttg aattgattca aacgactatc tttcattggt
     2281 aaattttgtg ccttaggtaa accaattttc gttgattttt cagttccaac ttcgattcca
     2341 ttatcatttg gcagaacgat ggaatcctca tccgtttgta cttcttgatt tcgttttgat
     2401 aaatctgaaa tgcgtttttc attttcggag ttaacagatc tataccgata ttgttctgga
     2461 tcagtttttg gtaactgtcc ctcattttct gctttgtctt cttgatttac ttttgataaa
     2521 tctgaaattc gcttctcatt ttcaaggtca ctgttaggtt tatagcgata ttgttctgga
     2581 tcagtttttg gtaactgtcc attttttcct tcttctgaat ttctttcttc tatggaattt
     2641 ttctcttcaa tcgaattttc attagagtgc aacgcttctt ggtgatcttt gactgaagga
     2701 atcactatac cttcttgatt ttctttttcg gttgttgtat tagcttttac tgctgcatta
     2761 acttgtcctt caccatacgc atcttttagt ccttctttga cttctgaagc tttttctttt
     2821 tgtttttcta gtgcttcgga tggattcttc acagcttcag ccaattcttt agctctcgtg
     2881 ccgtctaatt tctcttctaa ggtgtctttg gctttttctg taagatcttc agctgttcct
     2941 tttaacccac gttcattgac gtaaccaaga ccttctgcgg ctttagaaac accatttttc
     3001 gctttcccga acatttcgga aactgttttc ttactattag gtacagttgt agctttatcg
     3061 tctgattgtt cttccccgtt atcaggggta acatcttttc gatggccaaa tccattttta
     3121 cgaatatcat ttaaatggct taaaccactc ttaccagcac ttgcaaggtt tttagctccg
     3181 ctacccacat ttttcgcaat atttcctaag gcaaacatgc taagaagaga attactgccg
     3241 tttttcagac tcgtatcaac accgaaaatc ttcgtaaccg attgagagcc tttgaatagc
     3301 gcaatagtta gcacaattaa agcaacaaca tacaagaatc cattgatctt tcctgccgca
     3361 tgcaaatcac cgatacctga aagcattaat gtatagattc gtaattctaa aatcgtaaac
     3421 gccaataaca agaaggattg agaaatatct tctatcgcac ttttcatccg ttggcctgtc
     3481 tcaaagtccg ttgacgcggc aatcactaaa tagaattttt taaataccag ctcaataatg
     3541 caagtaacga tcgtaaatag gataaataaa taggccaccg ccaaagaaga ttcaccagct
     3601 aagataatac ctggtttact actgtatcga cgataccctg aagtatagac atattttgct
     3661 aagccttcgt cactaatttg aacaggaatt tctttcccat tatcatctag ttccaatcgg
     3721 tattgtaatg cctttaaccg atccttttgt aaatctgaca atccactttt atcgttatcc
     3781 gccatctcag cggcttgatc aggagtaata atcccattga tattcgctgt tttaaaattt
     3841 ttcgctgtta aagcatttct tttatctgtt ggtagggatt ctactttatt cgggtcagtt
     3901 gctagtaacg tactgacatc gatcacatta ttttgaataa tttgaaaagc aggtgatccc
     3961 gttttatctg ctttatttgc tgcagtgacg tctccgtaaa agcttttact gatatttaac
     4021 ccttcatcga ttaaagaact accaccaaaa atcaagatca tggccataaa taaattaacc
     4081 aagatatttt tgacttttgg tgggtgttta ctcataaaaa atttcaagcc taaataaatt
     4141 aatgtgacga cacaaaaaag agccaatacc ttactaatca aactttgaaa tagtgcattt
     4201 accccagcgg cgttcaataa gtcttttaaa tcaaagcttt gataaatggt tttttctagt
     4261 aaagtattga gattatataa tcctttaatc aatccccaca gaaaagtgtt ccaaatccaa
     4321 ctaaatacat cttgatactg cagataatta ccataatctt gtaagacttt cgttacggtt
     4381 ttggggtttg catccccttc tccgaataag cctgaccacc agtcggctat gccagaaaaa
     4441 attcctccaa tgatcattct ttcaccacct tttcagattc caataacaac cgtttcccaa
     4501 tcatttcaga aatttcttta tttttgatca acttttcaac atattcttga agtcgatctg
     4561 ctgcttgttc ttcgtaaaat tgaattaaca attttttagc cgtctcattc tcaa
//
LOCUS       DACDYJ010000133         4461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDYJ010000133 DACDYJ010000000
VERSION     DACDYJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.136"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            574..1008
                     /locus_tag="IWM51_002747"
     CDS             574..1008
                     /locus_tag="IWM51_002747"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972085.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="IWM51_002748"
     CDS             2298..3221
                     /locus_tag="IWM51_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9972086.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="IWM51_002749"
     CDS             3237..3776
                     /locus_tag="IWM51_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9972087.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          668 c          758 g         1371 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactcga cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DACDYJ010000134         4329 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDYJ010000134 DACDYJ010000000
VERSION     DACDYJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.137"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            126..563
                     /locus_tag="IWM51_002750"
     CDS             126..563
                     /locus_tag="IWM51_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972088.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            658..1542
                     /locus_tag="IWM51_002751"
     CDS             658..1542
                     /locus_tag="IWM51_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9972089.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1764..>4329
                     /locus_tag="IWM51_002752"
     CDS             1764..>4329
                     /locus_tag="IWM51_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972090.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SETATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIP"
BASE COUNT         1414 a          725 c          965 g         1225 t
ORIGIN      
        1 ttacttgatt tagttctttt tattttaaaa atttttttta cttagaatcc agccacccta
       61 gatatttttc tgtataatct taaataaaga aaacgatatt aaaaaatgca cgtagaagta
      121 gataaatgga gtggttttgc aaatttcttt tatttctaga agttattcac gtatacggat
      181 ttgtttttct cacaaataaa aaggcaggaa gtacaatgga tcatttgtat ataaaatata
      241 ttcatcaatt ttccgttttg gtattaggca cactaattat agcggggatt gttgcattta
      301 ttattcaagg tcgtgacata ttaacagtac atcaagagtt tttcagttat gcatttggta
      361 ttcctctgat cgctataagg tctgataaca aaaagtggaa gaaacgctat tacattctct
      421 tgctattttt gtgtttttat ctgccaccta agatttttac tttattaggt atacaatgcc
      481 tatcattttc ttttatttcc tatatcattg ttttaatttt cctcagtttc atagtatggg
      541 tattttatct tgtcaaaaaa taaatgaatg tttcatgaac agtacttcat cacagcctaa
      601 tttttttctt tatgctatcg ttatattaaa gaaatcaatc agaagggaaa gaaagtgatg
      661 aaaaaagttt tattagtggt aaaccccagt tcaggcggtg aacaagcaaa ggaatttgag
      721 caattagcga tagcaaaatt ggaatcggtg tttgatgaag tggtcgtctt gcatacaaaa
      781 aaagcagggg atgcaaaaaa ctttactcgc gaagcggcta cggaagggta tcatagtgtg
      841 tttgtcatgg gcggagatgg aacagtcaac gaaggaatca gtggaatcgc tgaacaagaa
      901 catcggccaa atttcggatt tttcccattg ggtacggtga atgacttggc tcgagcatta
      961 gggataccgc tagaacctga ggaagcgatc aatcattttt cgattgaatc agtcaagcca
     1021 ttggatattg gaaaaataaa tgacgactat tttatgaatg tcgtcgcaat tggctctatc
     1081 cctgaagcaa tcaatgatgt agatgcggag aagaagacga aatttggaaa actagcttat
     1141 ttcatgtcag gaatcaaaca gttagcttcc acacaatcct actcgtttca cgtagaagtt
     1201 gatggaaaga aggaagaaat tgagagcagt atcttgctga taggactaac taactcagta
     1261 ggcggttttg aaacattact tccaaatgca aaagtggatg atggtaagct ccatttggtt
     1321 tatttaaaag attcttcttt gatcgatacg cttaaaacat tgccagacct attaaaagga
     1381 gtagatgaat ctacgaaaaa tctagcctat cttacttgtg agcaattagt tgtgtcatta
     1441 gctgatgagg aagaattgac gacgaatgtt gatggagatg aaggggataa attacctgtg
     1501 acaatcaaag tgttaccctc acatttacag gtttattgtt aaaatggagg ttgtgaccga
     1561 agagttgtca ggaggacatc ggttgttcgg aataagggac tgtgacaaga cttttgtcac
     1621 agcctttttt tgtacaaaaa agaaattttc acctaacaga aaaatttgcc ataaaaatac
     1681 atattttaaa tatgaaagta caattacaaa taaattaaaa cgttaaaata aaattgtcaa
     1741 aacgtcagag aggatggtgg agaatgaatc aatatgtaaa aaagctgatt gcgtcgattt
     1801 tgtttgtctt ccctctcttg atggtgacat ccgtttttgc cgatgtagag aaaacaagcg
     1861 aatcaacgca atcagagaat caaagttttg acaagactac tttttcagaa gaatcaatta
     1921 cttctgaaac tacagaatcc caaagtgagg cgcctacttc taatacaagg caagaagaaa
     1981 ataagacaag tgagacctca gaagtagctg aacaaacgtc tgatagcagt gaagaggtac
     2041 aggttagagt tgtttcagcg gatgggtaca gcgaaactgc cacaacagaa gaactgacgc
     2101 agcttctcgc agacgaatca gtagctaaga ttcgtttaac tcagcctttg actttagaca
     2161 gggaattgga gatcaagcga gatatcgtta tcgattttgg cggacttgct cataatttcg
     2221 gcacacatca catctatatc aatgagacac ctcagtcgat cgagtttcag aattttaaag
     2281 gaacggcagc tcatccaggt gggttgatac caaatatcga tggaaatgcc attatcattg
     2341 cttatatgtc cgatttttgg ggaaacagat atcattttac gggagaaata aaatttaccg
     2401 ggacacttga cttatatgat ggttctaaat taggcctgat ttacgcccct cgtgcaactg
     2461 tcacattaga tggtgtttcc ggtgtattgg atgtccaacc agtgaaagaa gggatgaatg
     2521 ccgctccggg taaagcttat ttttgtagaa gctatcagct aaatgtaatc aatggtagtc
     2581 agctttatgg accatatcta ggaaaattct atggttttct agattcaggt gatgagacgg
     2641 gcagtggaaa aacagcacca ggactccata tcttgagtgg ttcaaaggtc tctcttgact
     2701 atgatcgggc agatggcgtc agcgatgaag gagaggctgt cgatacgcta cccgggaatg
     2761 tcacatttaa agtcagtggc tcaggaagtg aattttcggt aaatacaaag atcaatatta
     2821 ccaacgacaa caatcgcgga attatccaaa tgagaggctc aggatcaaga gtcgctgtgt
     2881 cgaatgatgg gaaaatcaca atcaatacag cgactactgg cggatttcgc ctgcaaggag
     2941 attctagtca aatccatgta tcaagtggtg gacaaatctc ggtcaaaatg gctggagatg
     3001 gcgaccaacc tggaaacaat gggatgcgtt ttgtagggaa aggactttct ttgatcgtag
     3061 aaggagccgg atcagtcatc gatattgata agcaaagtgg tcgttctagc gcagttcgtt
     3121 ttgagaacgg tacgcaaaaa ctaacagtaa ctaatggagg ctcattgaag gtaagaaatg
     3181 caggggatgg caaatcttat gtgaacagac tcaatcaagg aaaccaagcg atccaatttt
     3241 atgacgcttc tggttttcta gaaagtccag ggtcagctga ttttacggtt actggagaaa
     3301 aatcaaatat cgatatacgc gcagattccg gagctaccgt agatacaact ggagatattg
     3361 atttgaactt tatggctggc gaaaaaactt atttagtcat ggtcgggaaa actggggaag
     3421 ataagactgg catcatcagt ggggatgtgt tgaatgtaga cttagaatct cctacctatt
     3481 tcgatttcca aaacaaacgg atcggaagcg agactaatgg cataggtggc tgggtatttc
     3541 aaggaaatgg cgattcttct ttgaagatcg ctcattcaga aattgcttta tggcgaaagg
     3601 caggagtaac gttcgataac tttgaaaaag atccggatta tttttctgat atgacagact
     3661 tatcgatcaa tggaaccgac ttaggtaatt ttgtatcaag ccctagtgca ggtttaacaa
     3721 atgaatttgc tcagacaggt gccggaatga aaaattataa tcgaatcagt gccaataacc
     3781 agcttccgat gattgaatcc ttaagagtac caacgaatgc agataaaaaa atctatgggc
     3841 atgtcgttgt tcccgaaggt gtcgaggcaa agccaagaga tgcttggaca ggcgaagtag
     3901 aagtgacttt acgtattact tatgcagata cgacgaaatc acctgtagaa attactgctc
     3961 tcacacaagg acaaacagat gaaaatgaag ggacaggata taatccgtgg ggagaaggcg
     4021 aacaaggcgg tttgtttgag gttcaagtac ccaacgaaga aagactaaca acgggggatc
     4081 aagtgcaaat agttgctgct aaaaaagtaa gaggcggcca agaagtgaca gaactggact
     4141 ctgccaaaac gacagtcgat gtgattcctc ctgagcctgc taaattaagt accacattga
     4201 tcaatgcagc ttctcgctca atcacaggta caggcgaagc aggagctttg gtttcattga
     4261 aaaaaaatca agctgttttg tccgaaacaa ctattgatga tacaggtaaa tttgttcttt
     4321 ctatcccag
//
LOCUS       DACDYJ010000135         4220 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDYJ010000135 DACDYJ010000000
VERSION     DACDYJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.138"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            30..224
                     /locus_tag="IWM51_002753"
     CDS             30..224
                     /locus_tag="IWM51_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972091.1"
                     /translation="MIFAAVAAVASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVIL
                     AAASIVVSMFSMFGQTPQGL"
     gene            296..2350
                     /locus_tag="IWM51_002754"
     CDS             296..2350
                     /locus_tag="IWM51_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972092.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            2350..>4220
                     /locus_tag="IWM51_002755"
     CDS             2350..>4220
                     /locus_tag="IWM51_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP9972093.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNM"
BASE COUNT         1598 a          656 c          868 g         1098 t
ORIGIN      
        1 ctgccgcaac tagtttgaaa aataatctga tgatttttgc tgctgttgcg gcagtggctt
       61 ccattgctct tggaggtatt ttcttcttct ttgggcgaag aggagctgaa aacgggaaga
      121 cacacgtagg ttatgtattg ttgggggtag gagttatttt agctgccgct tctattgtag
      181 tttcaatgtt ctcaatgttt ggacaaaccc cacaaggact ataaaaaaaa caagtagaag
      241 gagtaaaaag tacgtggcta ctttttactc ctttttttat gaaaggagaa tattcatgtt
      301 ttttacgatt tcaggaaatg caaaattgga taaagagtta ataagaaaag caacgaagaa
      361 agtgggaaag cgtacttcct ttttaccgaa aacaattaaa tattcggtca ttgaaacgga
      421 agaacagcta catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt
      481 gactctcacg ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt
      541 tggcccactg gattatcaaa tttcagaagg tttttatcct acattgatgg atttaatccg
      601 ccttgatgtg tttaaagata cggatgaaac tatgagtgtg gaagagtctt atcgaaacga
      661 aaaactttta caagttttag aagaagagtt agagagcgac caaagaaaag tgaatcaagt
      721 gagtgaagtc gaagtaaatg aagaagtacc atgggaacga ccaatatttc caaagaagga
      781 gtcaaaagaa aaagagacag tctatgttga cgaacaagct gatattccga ctcagaaatc
      841 gatatttgag gaagagaacg atgtgatggg tcaattggag gtagatccct ttgaagaaac
      901 aatggaggat gaagagacgg acgaagtaga agttgatcca gttgaagcta atgaagagtc
      961 aacatggact gaaggggatt ctcaggaata tattgaacca cttatcagtg aagaagtaga
     1021 aaaaaacgaa acagagatac aaaaggcaac tgtatttgat tatattttgg atcaatatcc
     1081 gccagagcat gaatggatga aagaaaaaaa caaattattt ttaaatgagc tttattcgaa
     1141 atatcaatta ccccaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca
     1201 gggtaaaggc acacttttag aacagttaga gtctgtccaa tcaacaaatt ggcatgagct
     1261 tgcacaagaa gatttattag ccacctttga agaacgtcaa caagaaacag atcaagaaat
     1321 taatgagtat gcgacggaac agtcgtctaa ttgggagaaa gtaaaagagg aactaaataa
     1381 agaagaacag cgagtgattg aagaagaagt acaaaaaatc aaagagcggt acgaaaagaa
     1441 acgcgacaat gcttattcct tgtgtgtaga aaagatcaac aattttacta agacgaatca
     1501 agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa cgtattaaag aacgaaaaga
     1561 agagttctac cgcggtttac gtacagataa gctaaagatt agcaaagaat taaatgaaca
     1621 actgaataag ctatatcaaa caacgactga agttcttatg gaaaaacacg aacagatcca
     1681 gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag tatgaaattc aacaacaaga
     1741 attgaaagaa caagaagaaa aacaacggca aaaagaaaaa gagcgtgaac aattagaaat
     1801 aagaaaacgg gaacaggatc ttgcggaaca aaaaatggcg atagaaaaac aacggttaga
     1861 gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt caagaacaaa tggatacatt
     1921 gcgtcaagca cagttgaatt atctagcaca acctcaacca gtagctgctg ttcaaacgcc
     1981 agtgaacaac caagcaaatt taactccttc accccagcgt tcctttaacg gatggatggt
     2041 gggatgtatt gcaagtttaa gcttgcttct aggtggagga acagtatttg cttttaatca
     2101 tatcaatcaa tcttcacagg ctgattctgt agccacaacg gcgtcaatca aggaagaagc
     2161 ccaggaacaa gcaagaaaag agtatgaaca acaattagaa gaattattga atcgtaccaa
     2221 tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag agtgattcta gtaaggctaa
     2281 agaaggatca agcgattcaa ctgtacaatc aaacacttcc gaaagttcag aatcaaatga
     2341 ggtgaaataa tgcgtgttag agaaacaacg aagcgaaaag acaaacgatt caatttagaa
     2401 aagaataggt atctttctca tttctatcgc tatactccct caggagaaga gctaccagaa
     2461 aaacatgaca aacgcatttc gaaaaagatt ttttatagtg gacttgtata cctcccttta
     2521 gagtatttag taacaagagt accagaagtt ttcaaccgat tggaaattgt taagggaagc
     2581 ttacaaggaa taaatctgtt tcaattacag gaaatgccac aggtctttca ttttgattca
     2641 gctggttttt ggaatccatt tactcctaca actagtttgg gtacacgttt agcaatttcg
     2701 acagtttgtg tggttccagc tgtacttatg ggattgaaaa tggacatgtg gtttcgtcct
     2761 ctagcagatg gacaaaaagg ggataatcga ttaatgacct taaaagaaat ccaacagatt
     2821 tatccgcaaa tacctgaaac aaaaactact tttccaggtt atggaggtat tccaatcaca
     2881 cattacaaaa aatattggta cattcaaaca gatactgtta atacgtgtat tgttggtacc
     2941 tctcgatcag ggaaaggtca gattgaagtg ttagccacca ttgacaattt atctcgtgca
     3001 gaaaaacaaa gttcaatggt cgttaatgat ccaaaaacag aattatatgt tggtagtaaa
     3061 gatgaactag aagctagagg gtatgatgta tatgccttta atatcaccga tccactgcaa
     3121 tcaatgagtg ataatccatt ggcactcatc gtgaaatact ggaaacgagg agacatagat
     3181 actgcaacac agttaaccaa tactttcaca aatacgattt attatgatgc caacgcttca
     3241 gataacaaat actttaacga aaacgcgcag aaagctgtca atgcgcttat ttttgcgttg
     3301 ttagaacaag ctgatcaaac aggagattac tccaaattga caccgaataa tctcgttgag
     3361 cttctttctg agattggcgg ttttaattat tatccagatc cagacaatga attcaaacag
     3421 ttgaatgcct tagatgaatt tatgaatcag ttgccacctg gaaacattgc aaaaaaacaa
     3481 tatggggcaa cgaaaattgg atctgataaa gcgaagggaa atattttatc aacggcaatc
     3541 acgggattga atccatttac tctaacgaaa attgcaaaga tgacttctca atcaacgatt
     3601 gattataaat cggttgggtt tccaaaatac ttagatttga aattacatga aagtctttta
     3661 aatcaacgag tcgtagtaga attttatcat aagggaaaga aatttcatga agaacgtgtc
     3721 aaagtaggct ataaaggatt ttgtgaaatc aattttgatt gtcatttagt tgcaggtgat
     3781 caagtttgtt taaaatacga tcatcaagga aaagaatatg tggcttggta tgaattttct
     3841 caaagagtat tgaaagacga agagggtaat atcgtttatc ggaaaaaacg aggagaaacc
     3901 catttgccag aattagaaaa agaagccgtt ctaacattag atgaggagaa aagtaactta
     3961 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgcttaca
     4021 cctgattatg atccatctaa ccatatcatt gttagtattt ttcttagtca gttatataaa
     4081 gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgcag gattcatttt
     4141 attttagatg aatttgggaa tatgccacca atggataata tggaaggaat catgacggtc
     4201 acagctggcc gaaacatgtt
//
LOCUS       DACDYJ010000136         4217 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDYJ010000136 DACDYJ010000000
VERSION     DACDYJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.139"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            30..224
                     /locus_tag="IWM51_002756"
     CDS             30..224
                     /locus_tag="IWM51_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972094.1"
                     /translation="MIFAAVAAVASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVIL
                     AAASIVVSLFGMFGQTPQGL"
     gene            293..2347
                     /locus_tag="IWM51_002757"
     CDS             293..2347
                     /locus_tag="IWM51_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972095.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            2347..>4217
                     /locus_tag="IWM51_002758"
     CDS             2347..>4217
                     /locus_tag="IWM51_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP9972096.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNM"
BASE COUNT         1589 a          671 c          872 g         1085 t
ORIGIN      
        1 gtgcagcaac tagtttaaaa aacaatctga tgatttttgc ggctgtagct gccgttgcct
       61 ccattgttct tggggggatt ttcttcttct ttggccgaag aggagctgaa aacgggaaga
      121 tgcacgtagg ttatgtattg atgggggtag gagttatatt agctgccgcc tctattgtgg
      181 tttcgttatt tggaatgttt ggacaaaccc cacagggact ataaaaaata aatagaagga
      241 gtaaaaagta cgtggctact ttttactcct tttttatgaa aggagaatac tcatgtactt
      301 tacgatttca ggcaatacca aaaaagataa agagttaata aaaaaagcaa cgaagaaagt
      361 gggaaagcgt actgcctttt taccgaaaac aattaaatat tctgtcattg aaacggaaga
      421 acagctacat gtgttaggac aagtgttaga acaagagaat caatcttctg ttaaattgac
      481 tctcacgtta catgataaag aaggaaaaat agaaaaggct ggcacagaat acgtttttgg
      541 cccactggat tatcaaattt cagaaggttt ttatcctaca ttgatggatt tgatccgcct
      601 tgatgtgttt aaggaaacgg atgaaaccat gagtgtggaa gagtcttatc gaaacgaaaa
      661 actgctacaa gttttagaag aagagttaga gagcgaccaa agaaaagtga gtcaagtgag
      721 tgaagtcgaa gtaaatgatg aagtaccatg ggaacgacca atctttccaa agaaggagtc
      781 aaaagaaaaa gagacagtct atgttgacga acaagctgat attccgactc agaaatcgat
      841 atttgaggaa gagaacgatg taatgggtca attggaggta gatccctttg aagaaacaat
      901 ggaggatgaa gagacggccg aagtagaagt tgatctagtt gaagctaatg aagagccaac
      961 atggactgaa ggggattctc aggaatatat tgaaccactt atcagtgaag aagtagagaa
     1021 aaacgaaaca gagatacaaa aggcaactgt atttgattat attttggatc aatatccgcc
     1081 agagcatgaa tggatgaaag aaaaaaataa attattttta aatgagcttt attcgaaata
     1141 tcaattaccc caaacctatc aagcattttt agagagtaaa gaagaattaa tctcacaggg
     1201 taaaggcaca cttttagaac agttagagtc tgttcaatca acaaattggc atgagcttgc
     1261 acaagaagat ttattagcca cttttgaaga acgtcaacaa gaaacagatc aagaaataaa
     1321 tgagtatgcg acggaacagt catctaattg ggagagaaca aaagaggaac taaataaaga
     1381 agaacaacga gtgattgaag aagaagtaca aaaaattaaa gagcgatacg aaaagaaacg
     1441 cgacaatgcg tattccttgt gtatagagaa aattaataac tttactaaaa ctaatcaaga
     1501 agctttatcg aaagaaaaag aaaacttgtt aaaagaacgt ataaaagaac gaaaagaaga
     1561 gttctaccga ggtttacgta cagataagct aaagattagc aaagaattaa atgaacaact
     1621 gaataaactc tatcaaacta cgactgaagt tcttatggaa agacacgggc agatccagca
     1681 agaattacaa gcacaaattc caaaatggaa aaaagagtat gaaattcaac aacaagaatt
     1741 gaaagaacaa gaagaaaaac aacgacaaga agaaaaagag cgcgaacaat tagaactaaa
     1801 aaaacgtgag cagcatcttg cagaacaaaa agtggcgata gaaaaacaac ggttagagca
     1861 agaaaaggaa aaagaagcag ctgctcgtaa ggaacgtcaa gaacaaatgg atacattgcg
     1921 acaagcacag ttgaattatc tagcacaacc tcaaccagta gctgctgttc aaacgccagt
     1981 gaacaaccaa gcaaatttaa ctccttcacc acagcgttcc tttaacggat ggatggtggg
     2041 atgtattgca agtttaagct tgcttctagg tggaggaaca gtatttgctt ttaatcatat
     2101 caatcaatct tcacaggcag attctgtagc cacaacggcg tcaatcaagg aagaagccca
     2161 ggaacaagca agaaaagagt atgaacaaca attggaagaa ttattgaatc gtaccaatca
     2221 gtcaacagca gagtcaagta tggaaaatag caaagagagt gatcctagta aggctaaaga
     2281 aggatcaagc gattcaactg tacactcaaa cacttccgaa agttcagaat caaatgaggt
     2341 gaaataatgc gtattagaga aacaacgaga cgaaaagaca aacgattcaa tttagaaaag
     2401 aataggtatc tttctcattt ctatcgctat actccttcag gagaagagct accagaaaaa
     2461 catgacaaac gtatttctca aaagattttt tatagtggct tagcttatct tcctttaaac
     2521 tatgttgtta caagaatacc agaagcattt catcgatttg agattattcc aggaagttta
     2581 caaggagtga atatttttca gttacaagaa atgccgcagt tattccattt tgattcaact
     2641 ggtttttgga atcctttgac cccaacaaca agtttaggca cacgtttagt tgtttctacg
     2701 atttgtgttg ttccagctgt acttatggga ttgaaaatgg acatgtggtt tcgtccgcta
     2761 gcagatgggc aaaaaggaga taaccgatta aaaacgttaa aggaaatcca acaaatttat
     2821 ccgcaaatac cagaatcaaa gggaagtttt cctggttatg gcggaatacc aattactcat
     2881 tataaaaaat actggtatat tcagacggat acagtaaata cttgtattgt tggtacttcc
     2941 cgctcaggga aaggccagat agaagtttta gcaaccattg acaatttgtc gcgtgcagaa
     3001 agacaaagtt caatggttgt taatgatcca aaaacagaat tatatgttgc tagtaaagat
     3061 gaactagaag ctagagggta tgatgtgtat gcctttaata tcaccgatcc actgcaatca
     3121 atgagtgata atccattggc actcattgtg aaatactgga agcgaggaga catagacacc
     3181 gcaacacagt taaccaatac ttttacgaat acgatttatt atgatgcaaa tgcttcagac
     3241 aataaatact ttaacgaaaa tgcgcagaaa gctgtcaatg cgcttatttt tgcgttattg
     3301 gaacaagcgg atcaaacagg ggattactcc aaattaacac caaataatct cgttgaacta
     3361 ctttcagaaa ttggcggttt taattattat ccagatcctg agaatgaatt caagcagctc
     3421 aatgccttag atgagttcat gaatcagtta ccccctggaa atatagcaaa aaaacaatat
     3481 ggggcaacaa aaattggatc tgataaagcg aaagggaata tcttgtctac ggcaattaca
     3541 ggattgaatc catttaccct aacgaaaatt gcgaagatga cttctcaatc gaccattgat
     3601 tacaagtcca ttggttttcc gaagtatttg gatctcaaat tacatgaaag cctattaaat
     3661 caacgcattg tcgtggaatt ttatcataag ggaaagaaat ttcacgagga acgagtcaaa
     3721 gttggctatc gtggtttttg tgaaatcaac tttgattgtc atttagagca gggagatcat
     3781 gtttgcttaa aatacaatca tcaaggaaaa caatatgttg cttggtatga gttttctcaa
     3841 aaagtattga aagacgaaga gggaaaaatc gtttatcgaa aaaaacgagg agaaactcat
     3901 ttgccagaac tagaaaaaga agccgttcta acattagatg agaagaaaag taacctatta
     3961 gtagagtcta ttcggatgca ctataccgat aaaccaatgg ctatttttat gttaacgcct
     4021 gattatgatc catctaacca tatcattgtt agcatctttc ttagtcagtt gtataaagaa
     4081 ttatctacgc aatgtgttaa gactaaagga gataaatgtc accgtaggat tcattttatt
     4141 ttagatgaat ttggaaatat gccaccgatg gacaacatgg aaggaatcat gacggttacg
     4201 gcaggtcgta atatgtt
//
LOCUS       DACDYJ010000137         3788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDYJ010000137 DACDYJ010000000
VERSION     DACDYJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.140"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWM51_002759"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWM51_002759"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP9972097.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IWM51_002760"
     CDS             complement(1172..1867)
                     /locus_tag="IWM51_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP9972098.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IWM51_002761"
     CDS             complement(1880..2596)
                     /locus_tag="IWM51_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP9972099.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IWM51_002762"
     CDS             2926..3534
                     /locus_tag="IWM51_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP9972100.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
BASE COUNT         1088 a          775 c          674 g         1251 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 acccgtggtg ctagttaatt ttttcctaga ggaaaatgtc taaacatgtt ggtaaatcaa
     3781 caaaaaaa
//
LOCUS       DACDYJ010000138         3777 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDYJ010000138 DACDYJ010000000
VERSION     DACDYJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.141"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            141..1181
                     /locus_tag="IWM51_002763"
     CDS             141..1181
                     /locus_tag="IWM51_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP9972101.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2005..2337
                     /locus_tag="IWM51_002764"
     CDS             2005..2337
                     /locus_tag="IWM51_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9972102.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            2956..3297
                     /locus_tag="IWM51_002765"
     CDS             2956..3297
                     /locus_tag="IWM51_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9972103.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     NEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            3316..3606
                     /locus_tag="IWM51_002766"
     CDS             3316..3606
                     /locus_tag="IWM51_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972104.1"
                     /translation="MKFFEVKSPYYYALIAAKNEEECAELYEQVVTDIENREEFIRCL
                     HELNRNEAIQEDAKKISEETLQPIGMEEATKDIFTIIDEQKSCVLSIDSTLV"
BASE COUNT         1423 a          504 c          706 g         1144 t
ORIGIN      
        1 ttcattttat ttctgatttt aggtagtatt taattaaaaa acagttttaa tgcgtttgca
       61 ttttttattt atctttgcta ttttcgaaaa gagtaggtaa ttttacataa ataaaacaac
      121 gtgaaagtag aggatctaca atgactgatt ttaaattttt taaggcggat agagtttaca
      181 acgaattatt ttatcaattt ccaaaagtct ttattgtttc tgacgaatac aaaaaaatga
      241 aagattcaac taagattgcc tatatgcttt taaaagcaag attagagatc gcaatcagca
      301 aacggcaaat cgatgaagaa ggtaatgttt attttactta tacgacaaat gaactatgta
      361 gagtattaaa ctgccaaaaa caaaaagcga tagcaatcaa aaaagagttg gaatcctttg
      421 gtttattatt acaaaagcag atgggattta acaaacagtt agggaaaaat aatcctaata
      481 gactatatct agcagaatta aaagtctcag aaaatgatat ctacttactc gaaaaatttg
      541 atagagagaa tagggaaaac gttgataaat cagagggtat gaaaatcata cccaccctcg
      601 acgaaaaatc agacgctgaa tcccttgggg ctcaagaggg tatgaaaatc ataccgtgcc
      661 aaaacgttga taaatcagag ggtatgaaaa tcataccaga acttaataat aatatattag
      721 acactaatag acacaatata gacactgaaa aagaccgcct acaagatcaa ttgttgttag
      781 acaattttga gacaattatg acaaacgaca gcattgctac gtttgtccct gaacgagtat
      841 taaatttgat aaaaacattt tcttcaagtt acagtgaagc tcaaaaaacc gtccagacta
      901 ttcataatgc aaagaaaaaa gctgaaatag aaagtggtat ttcgatagtt tttgaagaac
      961 tcgatagtta ttatgtcaat gcagaacaag aattatacac gacactgtta aaagcctatc
     1021 aaaaattaaa aaccgaaaaa gtcgaaaata tccagaacct gatttttgtc tatgtaaaaa
     1081 attggtttat cgaaaaacca atagctgcta aagtatcaag tgaaaaacgt ttgaattatg
     1141 aaagctcccc aagcactatt acgaaagact ggttagagtg aaactttact gacaaagtga
     1201 ttgttgcttc atgaagtaac attaaaatta aaaatttttt attattctaa ttatattacg
     1261 aatattacaa aataatagct attttaatag aatttataag ttaaaatatt atgataaaaa
     1321 tcacaaacgt agccttttac gtcataagtg ttaaaagaaa taattaattt caaatataga
     1381 aaagggaacg tatgtttgca tactcctttt ttttgagtta taataatacg cgaagagaga
     1441 taatttcatt atttaaacaa gctaaaaatc ttgatattac ttcttttcag gcaatacgaa
     1501 tattattcgc ttacattttt tgtataaatt taattgtctg ctatgcttgt gatgaattga
     1561 ttttcgttct gcacttttgg atgataaatt tggacaattg aaaatacaaa aaaaagaaag
     1621 gcggtatgaa ctcggaaggc tggtaacctt cgtcagcgtt catacacggc attaagattt
     1681 ggcccttaat gcgttaacct ttcagtgtta cggtattacc cgcaatggat ttagtataac
     1741 agatattcaa aagtgatacc actttttttg gctgtgttaa ggttaaaaat acgtgatatt
     1801 acgatgaatc tatgcctaag cataatgtta taaaaatgtc cgtgtggctc tttgccgtat
     1861 gacgaacgct gaaattagag ggaacgcatg tttggatgcg ttccctcttt tttgtttgtc
     1921 atgaacgaaa atcaattctc tatttttgaa aaataacaag aaaaacttgt taaaaatatt
     1981 taaagaaatt gggtggttta ttttatgtca tatacaggaa ttttgagtct caaagatatt
     2041 tgtcactacg gtaaacggtg taccgctaca gagaaaatca cgaagaaact atcaactggc
     2101 caaagtaaaa ccgttgtgca gtgtaagaaa tatataatcc agaaagacaa agtctcagag
     2161 gaaatgattt attacattgg gaaacaaaag caaataattt taaaagatcc tatcccctta
     2221 aaagagctat atccaacaat caaacatgtt tatgaccaaa acggtgtact gattggtcga
     2281 agaaaaaatg gagtcttgcg ttgtaccgca aaagggatgg ggcgtttaat cagctgaatt
     2341 gatcattctc ataacttgta ttttatcgtt tttcgcttgc ttgaaaatat tgtcgagact
     2401 tctaaaagaa cttagaatca ttagaaaaat aaggagtaga cttgatctat caacaatttc
     2461 cttcggggag tgacggggta gatttttacg attggtaacg taaaaaattg gtcaatcgag
     2521 taaatgcgct tgtttggata gtggtgaaat gaatttgtgt aaataatata cacagagtaa
     2581 acaagaggga atttagaggt tagacaagtt agaatagcgc ctagattaat catgagcagc
     2641 tactaaggga tctaggcttt gacgcaaact catggaataa ggagtaggtc ttctcgtaat
     2701 ggggaggacg cacaagaaat tgtgtcgtgt gctgcagtac atgtagatac ttgcgaggac
     2761 gttggtcatt ggatcaatcg agcatttacc gctgtctact agggtttctc gtttcggacg
     2821 ccataaacga gtcgatattt tttatgtatt tttaagtaaa cgaaaaacga taaaatatga
     2881 acgagtcctg aataaagtga aaaaattgat ataaaacata gagaaaatga aatttacgta
     2941 atggaggaat gacaaatgaa aaattatgat ccgaatatac gattgggcac acatactatt
     3001 aaggtttctt tccaacgttg ggattacaaa ggttttgtga cgtttagaag aggcggaaat
     3061 tgtaaaggat tagatgtttt agcattaaac gaggatgatt tatacgatca aacattgact
     3121 gataatccaa ttggattcgg cttgctacct gaagatgatg aaggtaatga atggtttaaa
     3181 atgacgctta tgaatgataa tggagacgaa ttatctgttg aagatacttg gtcatatttg
     3241 agtgattata ttgtcagcgt tgaaatcatt gaatttgtag cggataaaga agaataaaaa
     3301 ctggaggaat aaattatgaa atttttcgaa gtaaaatcac catactatta tgcgttaatc
     3361 gcagcaaaga atgaagaaga atgtgcagaa ttgtatgaac aagtagtaac agatatcgaa
     3421 aatcgtgaag aatttattcg atgtttgcat gagttaaatc gaaatgaagc aattcaagag
     3481 gatgcgaaaa aaattagtga agaaacgtta caaccaatcg gtatggagga agctacaaaa
     3541 gacattttta caattattga tgaacaaaaa tcttgtgtat taagcattga ttctacattg
     3601 gtttaataga tccaagattc tataattttt ttcaaagtaa aaagaaatta ggtaatgatt
     3661 ctcagaccaa ttacttagga aaataaccat acaaattaga ttttacagat aaaggattag
     3721 aaggaatcac ggcgattaat aaaaaggtag tattacctta ggcattgagt aaggaat
//
LOCUS       DACDYJ010000139         3624 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDYJ010000139 DACDYJ010000000
VERSION     DACDYJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.142"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            746..829
                     /locus_tag="IWM51_002767"
     CDS             746..829
                     /locus_tag="IWM51_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP9972105.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            954..1691
                     /gene="erm(B)"
                     /locus_tag="IWM51_002768"
     CDS             954..1691
                     /gene="erm(B)"
                     /locus_tag="IWM51_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9972106.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1954..2070)
                     /locus_tag="IWM51_002769"
     CDS             complement(1954..2070)
                     /locus_tag="IWM51_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta-toxin"
                     /protein_id="HAP9972107.1"
                     /translation="MVQNQHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI"
     gene            2333..2845
                     /locus_tag="IWM51_002770"
                     /pseudo
     CDS             2333..2845
                     /locus_tag="IWM51_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            2894..3574
                     /locus_tag="IWM51_002771"
     CDS             2894..3574
                     /locus_tag="IWM51_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9972108.1"
                     /translation="MNHFKGKQFQKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKILYHLWKKKNRQSFYSWKMDETYIKIKGHWHYLYRAIDSDGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGQPRVIVTDKAPSISSAFRKLQINGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYRSLRTASTTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
BASE COUNT         1197 a          597 c          687 g         1143 t
ORIGIN      
        1 ttgacaacca tttttgttag gcgtttgagg aagtgaagct gttttcctca tgttttaaaa
       61 gctagaaata aattacagtg tacgctcctt tcttttttcg ttttgttggt tcattttcgc
      121 cagtgtgttc gtgtcgtttg ctgtgacgag tacgcaacat agacacaaag aaaacacaag
      181 ggcgagcttg ctcgtttata tacccttgtg ttttcgactg gatatgttaa ggtaacaaac
      241 agtaaatgac acgttacaga ctgcaaaatg taccaactga aaaaagaaag aataccgttg
      301 tgtgttttat ttctcgatca gcttttaaaa catggggtcc cgagcgccta cgaggaattt
      361 gtatcgataa gaaatagatt taaaaatttc gctgttattt tgtacattta acttgacggt
      421 gacatctctc tattgtgagt tattagtggt acagttttca accgttttaa ttataaaaaa
      481 gtggtgcatt tttaaattgg cacaaacagg taacggttat tgcaggtgta tttcttatct
      541 atgggtttaa catggatttt atcattaaaa tcatgagtat tgtccgagag tgattggtct
      601 tgcgtatggt taaccctaaa gttatggaaa taagacttag aagcaaactt aagagtgtgt
      661 tgatagtgca ttaaaaattt tgtataatag gaattgaagt taaattagat gctaaaaatt
      721 tgtaattaag aaggagggat tcgtcatgtt ggtattccaa atgcgttatg tagataaaac
      781 atctactgtt ttgaaacaga ctaaaaacag tgattacgca gataaataaa tacgttagat
      841 taattcctac cagtgactaa tcttatgact ttttaaacag ataactaaaa ttacaaacaa
      901 atcgtttaac ttctgtattt gtttatagat gtaatcactt caggagtgat tacatgaaca
      961 aaaatataaa atattctcaa aactttttaa cgagtgaaaa agtactcaac caaataataa
     1021 aacaattgaa tttaaaagaa accgataccg tttacgaaat tggaacaggt aaagggcatt
     1081 taacgacgaa actggctaaa ataagtaaac aggtaacgtc tattgaatta gacagtcatc
     1141 tattcaactt atcgtcagaa aaattaaaac tgaatactcg tgtcacttta attcaccaag
     1201 atattctaca gtttcaattc cctaacaaac agaggtataa aattgttggg agtattcctt
     1261 accatttaag cacacaaatt attaaaaaag tggtttttga aagccatgcg tctgacatct
     1321 atctgattgt tgaagaagga ttctacaagc gtaccttgga tattcaccga acactagggt
     1381 tgctcttgca cactcaagtc tcgattcagc aattgcttaa gctgccagcg gaatgctttc
     1441 atcctaaacc aaaagtaaac agtgtcttaa taaaacttac ccgccatacc acagatgttc
     1501 cagataaata ttggaagcta tatacgtact ttgtttcaaa atgggtcaat cgagaatatc
     1561 gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa acacgccaaa gtaaacaatt
     1621 taagtaccgt tacttatgag caagtattgt ctatttttaa tagttatcta ttatttaacg
     1681 ggaggaaata attctatgag tcgcttttgt aaatttggaa agttacacgt tactaaaggg
     1741 aatgtagata aattattagg tatagggcac ctctaataac taccaattaa ttcacctcaa
     1801 aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt tcttatcgat acaaattcct
     1861 cgtaggcgct cgggacccca acatgaggaa atgtttttat aaaaaaagag tagttaccgt
     1921 tttttggtaa ctactctttt ccttttttaa ggtttaaata cctggaagtt taggtgtttt
     1981 gggtattggt ggtgtaggtg gctgcaagct ttccaatttt tgttgaattg ctttaaattc
     2041 aggggtttct tggtgttgat tttgaaccat tttttgctct attctctcca aagtcggttg
     2101 aatttctttc cctgatactt tacgatttaa ttctctttct aaggtctctt tcggactaat
     2161 ggaaggcgtt tctaagcttg aatagagttt cactccttct ctattgtaaa gccttatatc
     2221 gctaaataag cccgttttat gaagggtttc taaattggtc ggtaagtttt tgacaacaat
     2281 atcatgcgct tgttttggtg gtgccctagc tgtcattgga tcatctgcat acaataagat
     2341 ccatgaaagg ccatctgctg ccaatgagcg ccttgagatt ggtaattggg aagctgatac
     2401 tgtagccggt aaaacaggta gggctcgtat tgtaactcta gtcgatcgta gatctcgttt
     2461 ctccctactt ggtaaggttg aaaagaaaat ctctaaaaat gtggttgatt gtatgatcaa
     2521 tctactaaga aatgtaggag aagataaatg taaaaccgtt acacccgata gaggaaaaga
     2581 attctcccaa catagtcgaa taacgctaga attaaatggc actaaagtgt attttccaga
     2641 tccacatgct ccatggcaac gtggtacaaa cgaaaatact aatggtttat tacgagaata
     2701 ttcacccaaa ggtgaagata tttcaggtat tttcgatagt caaattcaag aatgggcgaa
     2761 taaattaaac acgcgaccac ggaaatgtct aaattggaaa accccttatg aagtattttt
     2821 ggttctgttg caaagttccc cataactcct gatttattgt aaaatgtaag aaaacgagag
     2881 tgagggtaga cagatgaatc atttcaaagg gaaacaattt caaaaagatg tgattattgt
     2941 cgctgttggt tactacctgc gttacaatct gagctatcgt gaagttcagg aattactgta
     3001 tgatcgcgga ataaatgttt gtcacactac gatttatcgt tgggttcaag agtacagtaa
     3061 aatcctctat catctttgga agaagaaaaa tagacagtcc ttctactcgt ggaaaatgga
     3121 cgagacctat atcaaaataa aaggccattg gcattatctc tatcgtgcga ttgattctga
     3181 tggattaact ttagacattt ggttacgaaa gaaacgggat actcaggcgg cttatgcttt
     3241 cttaaaacgg ctccataaac aatttggaca acctagagtg attgtaaccg ataaagcacc
     3301 atcgattagc tctgcattca gaaagctaca gattaacggt ttatatacca agacagaaca
     3361 tcggacagtt aagtatctca ataatttaat tgagcaggac catcgtccaa ttaagcgccg
     3421 taataaattt tatcgaagtt tacgaactgc ctcaaccacg attaagggca tggagactat
     3481 tcgaggaata tacaaaaaga accgaagaaa cggaacgctc ttcggatttt cggtgtcgac
     3541 tgaaattaaa gttctgatgg ggatcccagc atagtcaaaa tattaaacta ggacttctat
     3601 actcttttgg ggaactttgc aaca
//
LOCUS       DACDYJ010000140         3550 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDYJ010000140 DACDYJ010000000
VERSION     DACDYJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.143"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            14..307
                     /locus_tag="IWM51_002772"
     CDS             14..307
                     /locus_tag="IWM51_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9972109.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFIYDLSLASSCNMSDVYEKLLILENEYDQFNSNQKKKKKKHID"
     gene            626..1468
                     /locus_tag="IWM51_002773"
     CDS             626..1468
                     /locus_tag="IWM51_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9972110.1"
                     /translation="MNANSIVVGNFKGGTGKSTSTQMLGFVNAFYKKRKTLLIDLDPQ
                     ANASEVMSFTADNLGNHFYQENGFPKTIWNVLYDGSLDGAVLPLIENLDLIAGNIAMS
                     DYSDFMSAKFPNDKLAQFEYFANILAPLKEEYDEIFIDVPPSLGTIVQSAMYFAEYVA
                     IMLQTQPKSIRGAADYIDYMEFFTERYNTQLGIAGIIPFMLDTKTSTESYMYNEAKKL
                     YGDNLINTIVFRSARLIRYDETGITLETKKNGELKKVDANPQNTFISILDELNEHISW
                     FEEE"
     gene            1471..2025
                     /locus_tag="IWM51_002774"
     CDS             1471..2025
                     /locus_tag="IWM51_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972111.1"
                     /translation="MAERQSKLFPNKKARPRTNGMKDMEEFTKSLSISSADNSAPKVN
                     TTEKHSDDNKNLKNPNKSHKGKAGRKAEYKDPRLKKDLNTKISLSTKLRIQRLISRKF
                     DNKSEGDVIDIALDYLVSSFDRDDRDSLYKAYKEDMESMIPIIQEKNEKAKKQGKLTL
                     DITDEINQQTLKEQKEKWVSAKFE"
     gene            2673..>3550
                     /locus_tag="IWM51_002775"
     CDS             2673..>3550
                     /locus_tag="IWM51_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP9972112.1"
                     /translation="MSTEFQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHKA
                     EELEKTVVVYEELESYG"
BASE COUNT         1452 a          495 c          522 g         1081 t
ORIGIN      
        1 aggcttacgc accatgtcta tcaatacatt tgtttactcg cacccgatca atgtttacat
       61 tatcaagaat ttaggaatca ctgtagaaca attttgtgag ttatatgcat acccacaagg
      121 aacggttgca agctggatta ctcgacaacg tagaatcaaa tcactacctg cttcatttat
      181 atatgatctg agtcttgctt ctagttgcaa catgtctgat gtctatgaaa aattattgat
      241 actagagaat gagtacgatc aatttaattc caatcaaaaa aagaaaaaga aaaaacacat
      301 agattaatat tgaaacgaaa aatagtattt attttaaaaa aagagaaagt agaaaaagat
      361 atttttccat tatttgatta aagttacttt taatcaaaac tattgtaaaa tagtacttgt
      421 tttatgtatc ttaaaatcaa aaaactttga ttaaagattc ttcaatcaaa aaaacttaaa
      481 aacattgata taatagcatt taataaaaat aagtatctcg taatcaaaaa aataatttga
      541 ttacgagata cttatttaca tttacaacag caagttgtat acttaatttg aataaaagat
      601 acttaatcaa aaggagcgta ataatatgaa tgcaaattca atagtagttg gtaattttaa
      661 aggaggaaca ggaaaatcta cttctactca aatgctaggc tttgtaaatg cattttacaa
      721 aaaaagaaaa acattgctta tagatcttga tccacaagca aatgcttcag aagttatgag
      781 tttcactgct gacaacttag ggaatcattt ttatcaagaa aatgggtttc ctaaaactat
      841 atggaatgtt ctttacgacg gtagtttaga cggcgctgtc cttcccctaa ttgaaaattt
      901 agatttaatt gccggtaata ttgctatgag tgactactct gattttatga gcgctaaatt
      961 tccaaacgat aaattagctc aatttgagta ctttgcgaac attctagcac ctttaaaaga
     1021 agaatatgac gaaattttca ttgatgtacc accttcactc ggaactattg tacagagtgc
     1081 aatgtacttt gctgaatatg tagctattat gcttcaaaca cagccaaaat caattagagg
     1141 ggctgctgat tatatagatt atatggaatt tttcactgaa agatataata cacaactagg
     1201 tattgctgga attattccat ttatgttaga cacaaaaaca agtacagaaa gctatatgta
     1261 caatgaagct aaaaagttat atggcgataa ccttatcaac acaatcgttt tcagaagtgc
     1321 tagattgatt agatacgatg aaactggtat tacattagaa acaaagaaaa atggggagct
     1381 taaaaaagta gacgcaaatc cacagaatac tttcatatct attttagacg aactaaatga
     1441 acatattagt tggtttgagg aggaataaag atggctgaaa gacaatcaaa actatttcct
     1501 aacaagaaag cacgtccaag aacgaatggt atgaaagata tggaagaatt tacaaaatct
     1561 ttatctatat cttcagctga taattccgct cctaaagtta atacaacaga aaaacattct
     1621 gatgataaca agaatctaaa aaatccaaat aaaagtcata aaggtaaagc tggcagaaaa
     1681 gctgaataca aagatccaag actaaaaaag gatttgaata caaaaatctc tttaagtacc
     1741 aaattaagaa tccaacgcct tatttcaagg aaatttgata ataaatctga aggtgatgta
     1801 attgatattg ctcttgatta tttggtatct tcatttgata gagacgatag agattcattg
     1861 tataaagcat acaaggaaga catggagtca atgataccaa ttattcaaga aaaaaatgaa
     1921 aaagctaaaa aacaaggtaa actaacacta gatatcaccg atgaaataaa tcaacaaact
     1981 ttaaaagaac aaaaagaaaa atgggtatca gctaagtttg aatagaagat acttaaaaca
     2041 acataaacta ttgattgaag tatctggtaa tattgatttt aagatacctc aatcaatagt
     2101 tttttcttta aaataagcat ttatgtttga ataagagata ctaaaatcaa ataaaagtat
     2161 cttttaatca acaaactagt ttgaataaag ttactcataa tcaaattata aatgattttt
     2221 agatactaca atcagataga aaccctatta aattaacttt cttaattgat taaaagatac
     2281 taaaatgaga ttattttgat tttagtgcca aaaaacaaac taaaattttt gattttagta
     2341 tccttaaatc aaattaaatg tatattacga gtcactataa tcaaaggtaa tttattacga
     2401 atattgtctt ctcaataaaa agttcttgat tataagatct ttcaatcaga tttatttttg
     2461 aataaggtgt cttccaatca aaagtaataa ctgatttaag tatctcttat tcaaaatata
     2521 gttgtctgaa aataataaac tgttaaactt attttgaagc ctatattatt cctgatttgt
     2581 atatcgaaat tcattagcta tatacattta aagaaatcta attatatttt tgataataaa
     2641 aattgtacgg atactagaaa agagggagtt taatgtctac agaatttcaa tctcaaaaaa
     2701 tgattattaa aaattgggga tataaagcaa caaatgttta tattgaaaaa tattataaat
     2761 ttcctagttt tttaattcag aattctaaat attcaaaaat gaaagacagc tcaaaaatag
     2821 cttatatgct ttttaaaaat gaatttcgta gagctttgca tgaaaaatgg atcgacgatg
     2881 atggactatt atatttggaa tttacacaag ccgatctaat ggataaatta aattgctatc
     2941 aaggcaaagt aaaaaccatt ttaaacgagt tagaaacata taatttgatt tctgtcgtaa
     3001 aaggagaatt taacccaaaa acgaaaaaaa atgaaaaaaa taaatactat ttacttcaac
     3061 ctgaaataag tgccgatgat ctttttattc aaaattcttt cgatgacgat ttaagcgaaa
     3121 ctattgataa atcaacctct tctcggaatg cggaaatcgc acgtcgagag gaaactgcta
     3181 aaacccttga taatactgga aatgcggaaa tcgcacgtcg agaaaaaaca gctgaaaccc
     3241 ttgataacac tggaaatgcg gaaatcgcac aagattatta tattaataaa tctttagaca
     3301 ctaaaagaca cttaaaagac actgaaacag accaattaca aaatcaagtt ttgctagata
     3361 acttcgttga aatcatgaaa gaagacagca taaacacatt tgtaccagaa aaggtattaa
     3421 gcttgataaa aacattttct aacacttacg aagaagccca acaaactgtc accacaatac
     3481 acaacgcaaa acataaagcc gaggaacttg agaagacggt agttgtgtat gaagaacttg
     3541 aatcttatgg
//
LOCUS       DACDYJ010000141         3227 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDYJ010000141 DACDYJ010000000
VERSION     DACDYJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.144"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            152..514
                     /locus_tag="IWM51_002776"
     CDS             152..514
                     /locus_tag="IWM51_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9972113.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILNASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIHVFLKLSENLSMKS"
     gene            531..1148
                     /locus_tag="IWM51_002777"
     CDS             531..1148
                     /locus_tag="IWM51_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HAP9972114.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGLRQIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVALVGEEEEEEEEVVEKLE
                     SRGTNRFFWTVALITIASGGDNLGIYIPYFASLSFSEIVTALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERILVPIVFIGLGIYILIENGTIQTVLSLFN"
     gene            1388..1939
                     /locus_tag="IWM51_002778"
                     /pseudo
     CDS             1388..1939
                     /locus_tag="IWM51_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            2252..2794
                     /locus_tag="IWM51_002779"
     CDS             2252..2794
                     /locus_tag="IWM51_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972115.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
BASE COUNT         1050 a          537 c          604 g         1036 t
ORIGIN      
        1 taaaaaattt aataataaaa tttaaattcc tattaagttg taacacatga aattgcttat
       61 aatagtgtat tgaaattaag gtagattgac aaacgagtga tcaaatagat atactacctt
      121 caaacgaccg tttgaatgtg aggtgagatg aatggaaaaa gtttgccaag tgactatcgt
      181 acataaagaa aaagtaaatg taataaaaaa aaaattggat caaaaagatt tttcaaactt
      241 acttgtgtta gggaaatgtt tcagcgattc ttctagaatt aaaatttttt atgctttaga
      301 aacctataag gaaatgtgtg tctgtgattt agcagaaata cttaatgcta gtgtcgctac
      361 aacgtcacac catttacgtt ttttaaaaaa acatggaatg gcaaaatcac gtcaagatgg
      421 aaaagtagtt tattactctt tagcgaatga agacatcctt tccgctattc atgttttttt
      481 aaagttatca gaaaatctat ctatgaaatc ttagtttgga ggaagttatt ttgttacaaa
      541 gtattttttc agctatcgct gtttatattt ctaccagcat tgattatttg tttatcttgc
      601 tgattatttt ttctcaaagt catacaaaaa aagggctccg tcagattttt tggggtcaat
      661 atctaggaac aggtatctta gtagccgtaa gtttatttgc agcatatgtg ctgaatttca
      721 ttccgcaaga ttggatcatt gggcttctcg gtctaattcc gattttctta ggaatcagag
      781 tggcattggt tggcgaagag gaggaagaag aagaggaagt cgttgaaaag cttgaatcaa
      841 gaggaacaaa ccgcttcttt tggaccgttg ccttaataac aattgcttct ggtggcgata
      901 acttagggat ttatattcct tactttgcct cgttatcttt ttcagagatt gtcaccgctc
      961 taatcgtatt tgctatttct gttgcggttc tttgctatat cagttacaaa ttagccaaga
     1021 tttcttttgt ttccgaaacc ttagaaaaat atgaacgaat ccttgttcct atagtgttta
     1081 ttggcttagg aatttatata ttgatcgaaa atggtacgat tcaaactgta ttgagtttat
     1141 ttaattaata aatagtttga ttgttagtac aaaaaaagac agcgattaaa actcgttgtc
     1201 ttttttttgt actaacagat tggcgcatta tctaagtaat ataattcgat acatgaaatt
     1261 acataaaatc atgtatcaag aatcctatcc ttatagggtt cttttttttt actcccccta
     1321 aacacacgat ttcgtgttgt aataactata atcatgtatt cgaacagact aaaaaggagg
     1381 gattgtcatg ggctataatg ttcagccact acggtcgcaa caggagatta atgatttttt
     1441 attttgcctg cgacggaata aaaatgcgga acgtgacgtt ttcttgtttc taattggaat
     1501 taacagcggt ctgcgcatgt ccgacatcgt taaactcaag aaaaaagacg tgattacctc
     1561 aaaaaacccg cgcatcgtgg agcaaaagac agggaaaaca cgcattttat acttaagcag
     1621 cttacaggac ttgattcacg gctaaaccgc acccttagat ccggaggctt acttgtttcc
     1681 cagtactaag ggcggccatt tagaagtcaa tacggtctac cagatgtttc aaaaggtcgc
     1741 gaagctgtta ggaagggacg atatcggcac ccacacccta cgaaaaacct ttggttacca
     1801 ctattacaag aaaaccaaag acgtggccac cttaatggaa atctttggtc acagcagcga
     1861 gaagattacc aaacgctaca tcggaattaa tgaagatgaa atcagtgaaa cgttattaaa
     1921 ttttcggtta ggtttttgat tgtattatat aatagataag cacagtaaga cggttataat
     1981 cacccacagt attggacaaa tcaacctttt tttggtatat tatatataca aatgagagca
     2041 ggctcatagt caacttgata taaatccgga acataagggt ccggcgggtg gcgagcacca
     2101 aaactaaact cgatacaaat ccggaacgta agggtccggc gggtggcaag caccaaaact
     2161 aaacctgtac aaaaggctgc tcttttgagc agcttttttt attgggaaaa aggagcattc
     2221 ttcgcacgcg gcaaaaaaag aatacaaacg catgtctata aaagagacaa cccaaattac
     2281 cagacagtta aacgcgattt ataaggctgc tcatctctta caagaacatt ttgtagataa
     2341 aaaagtatcc tttgtcggag aagtctcaac ggtagcaatt attttttcga caactaattt
     2401 tatgcacctc tgtggcattg attatcgcag aggcacacac ttgttttttc aagatgcctt
     2461 agacaggaaa ataaatttac aggatattca aatcaaaacg gatgggacga cctttcagaa
     2521 attacaagta ataggtagcc ttgatttatt attggggaag catatttcta ttgttggcag
     2581 aggtgtttac tcttctcttc gctatgatgc agctattcga acaaggaaaa aaatattggc
     2641 tctgtctttg aaacaaaacg gattaatcta tatccctatc tctttgttaa acctatcatc
     2701 aaaggaaatt ggacctggac aaaaagttac tggcattttt agcgaggatt taacatccgg
     2761 ggaacttaaa atgattatgg aagtcatcga ttaatcatta atagtgatat gcgacgtcat
     2821 tttgaagtaa ttattctatt ttgggttata aaataaagca ttttaagacg atttcaaatc
     2881 attcatgtgg gttatttatc acggatgagc ttgaaatcgc cttttatacg tatcaaattg
     2941 aaatgttcgg catacaatct atcgttgtta ctacattact aattgtcact tgttaaggaa
     3001 cttggatcct ttctaaacat tccctagaga taaattttac tttatccctc cgttaatatc
     3061 caatttaagc acctttccga ttcagggtta atttgcatat caagtgcagc ttgtgttcta
     3121 gtaagattag cccttttagt ccggctgagt tgacttatgg atataagata tggtcatagc
     3181 aaatagatac tacgtatagt ctgttaattt agtgtcatta atcttaa
//
LOCUS       DACDYJ010000142         3150 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDYJ010000142 DACDYJ010000000
VERSION     DACDYJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.145"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..268)
                     /locus_tag="IWM51_002780"
     CDS             complement(<1..268)
                     /locus_tag="IWM51_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972116.1"
                     /translation="MTKIYFMLTSNSGVGSTTIFATRARRNIDDLLAFFSQYYDVSAN
                     YTKDKDMIDLLVLPEPFGPFNNERNLPVIQIPTILFLERDFEKIK"
     gene            369..800
                     /locus_tag="IWM51_002781"
     CDS             369..800
                     /locus_tag="IWM51_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9972117.1"
                     /translation="MLNKRIANKNDYKLVLEIWEKSVKQTHDFLAEADFNFYKAIIPE
                     NLDEVKLYLWEDNGEIVGFSGVSAEELVMLFLDPDFIGKRYGSQILIELIETEDIKRI
                     DVNSQNKYAKHFYLSHGFEIESEEDLDGFGKPYPITHLVKK"
     gene            1214..2032
                     /locus_tag="IWM51_002782"
     CDS             1214..2032
                     /locus_tag="IWM51_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9972118.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(2105..2464)
                     /locus_tag="IWM51_002783"
     CDS             complement(2105..2464)
                     /locus_tag="IWM51_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9972119.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT         1107 a          441 c          552 g         1050 t
ORIGIN      
        1 tttttatttt ttcaaagtct ctctccaaaa ataaaatagt cggaatctga atgaccggca
       61 aattacgctc attattaaac ggtccaaagg gctctggtag caccaataga tcaatcatat
      121 ccttatcttt ggtataattt gctgatacgt cataatactg agagaaaaaa gctaaaagat
      181 catcaatgtt acgtctagcg cgggtcgcaa atattgtggt agaaccaacg ccactattag
      241 aagtaagcat aaaatagatt ttagtcatta cgaattctcc tttttaaata aaatatagcg
      301 gtagctttat gtttgactaa ctatgcttgc aaataaagtt aaaattataa gaaaagggga
      361 ataagagcat gttaaacaaa agaatagcaa ataaaaatga ttataaatta gttttagaaa
      421 tttgggagaa gtcagtaaag caaacccatg attttctagc tgaagcagat tttaattttt
      481 ataaagcaat cattcctgag aacttagatg aggttaaact ttatttatgg gaagataacg
      541 gagaaattgt cggttttagt ggtgtaagtg cagaagaatt agttatgcta tttcttgatc
      601 cagattttat tggtaagcga tatggtagtc aaatattaat tgagctgatt gaaactgaag
      661 atataaaaag aattgatgtt aatagtcaaa ataaatatgc aaaacatttt tatttaagcc
      721 atggatttga aattgaatct gaagaagact tagatggttt tggtaaacca taccctataa
      781 cgcatttagt aaaaaaataa ttcatgaatg gtttattacc tgattgaaca tgaataaaag
      841 taatattttt attttgatgg cctatttcta ggttatgaat tatccagatc aaatttgtgt
      901 attacattca gtaataaggt gcttatctta ggatcaactt atgatactag ctgaccaagg
      961 atgtaccagt tatagaaaat cagttaaagt aaaaatgata aaaatcttat gtcaacactg
     1021 atttaggctg acctgtgggt taggagaaag acaggtcggt agcttttaat acaaaaaatg
     1081 gcttgtatgt atctagatag ttaactgaaa ttagaaatgt tcttattttt aacggagtaa
     1141 gaaaaatagg ctgctttagg tctgaatact ttttcaatat aatgaagata tcaaaggcga
     1201 caggagtgtt tttatgaata agaatcctca taatctaaag ttggtagcag tagatatgga
     1261 tggaacattt gctcgtactg attacacata cgatatccca aggttcaagg ctgttttatc
     1321 gcgaatgaaa gctgttgatt gtcaatttgt tgttgcaagt ggaaatcaat actaccaatt
     1381 gagatcgtta tttccaaatt attatgatga attaagtttt gttgctgaaa atggtgcttt
     1441 tgttaaagat aaaacagagg tactattttc agccgatatg cctagaaaag cgatcgaggt
     1501 ggtccttgat ttatgtaaag actatcctga agtaaaaaat gtgatgtgtg gtctgaatag
     1561 tgcctactgt gaaagaggaa aggtgagtca agcatttttt gatctaacca atatctatta
     1621 ccatagatta gaatgggtag ctgattttgc agaagttcaa gataaaattt taaaatttgc
     1681 tccgactatg cctgttgaaa aaaccgaatt ttacgttgaa ttgttccgtg aaaaattagc
     1741 cgggctatta gtacctacca gtagtggtca tggttctgta gacttgattc tttctgagtg
     1801 ccataaaact ttcggcttga aaatgttagt tgagcgcttg gggattaatc ctgaccagtg
     1861 cgttgctttt ggagatgggg gcaatgacat tgagatgttg gagtattgtg gtctgagtta
     1921 tgaaatggat aatgcaacgg aagctgttaa gcaagttgct aagcatcaat gtccttcaaa
     1981 tgacgaagac ggtgttttag tgacgctcga tcgattgttc cctaacacgt aaacactagg
     2041 taattcatca aaaaacctcg atataagaaa tattcgaggt tttaaaacta tatttagaac
     2101 agatttacga agcgtctgat gagtcaaaat agacttggac attgctatct aaaataccac
     2161 tttcattctt taaacgaatt gtttgatttg atggagttac ctctaatact aaatgcccat
     2221 taataaaggt agtatcgtat tcagatgttt taactgttgc ttcttcgcaa ggtattgtta
     2281 aatcatatgc tggaatataa tgttgtgtgg aaactaataa aaaatgatca ccaatcatac
     2341 agcatccctc tgctgaagaa aattgattag agccatcatt taaagttaac aagaatccat
     2401 cctttaaacg aggtacttgc ttaaatactt ctggttgtac ttctaaaaat aaattatcgc
     2461 tcattttcac gaccaccatc gacttccaca gttgaaattg acattttatt taacacacct
     2521 attccccctt aactataact caattctata taaaaaatat tctattctca aataattgaa
     2581 cttttcaaat cgccaattta cttcggcttt atcagagctc attcatatca agagcgatta
     2641 acgctgtaaa aattaaaccg aagatctgat aaattttctc tattttctac aatatttaaa
     2701 acgcttcctt agtctattat atatctattt ctcttaggag tatgtttaga tgtttgaata
     2761 atagttgccc aactttaaag gttattctta taatatttta cttcattttc caatagaaaa
     2821 ttcaaaggat tactggtttt tctaaaatag tttagtttaa atacataagt atcctccgac
     2881 actgtattag ttcataatca tctaaataca aaattttcaa ataaaaatat ttcaagctct
     2941 atcgttgacg aatgtaaacg atacttataa agtaatagaa attgattaca tttgtaaact
     3001 taaaatggtt aatttatctc acataaaact aagaataaaa taatgattaa aagtggttag
     3061 tttttatacg atttagccta tcggtaaaaa tgaattagag agtgtaaaat attttgtgta
     3121 aatgaaaaaa tccatacaaa aaaggaagtc
//
LOCUS       DACDYJ010000143         2945 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDYJ010000143 DACDYJ010000000
VERSION     DACDYJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.147"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            232..1500
                     /locus_tag="IWM51_002784"
     CDS             232..1500
                     /locus_tag="IWM51_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HAP9972120.1"
                     /translation="MGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKSVEEIPSPVT
                     GTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETSGSASVVEAA
                     DPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPSSAPAKSEAP
                     EAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVNSKHTAPHVT
                     LHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLNASIDDANQE
                     IVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDGKLSAEDMRN
                     GTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGRVMKLSLSFD
                     HRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1508..2914
                     /gene="lpdA"
                     /locus_tag="IWM51_002785"
     CDS             1508..2914
                     /gene="lpdA"
                     /locus_tag="IWM51_002785"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP9972121.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
BASE COUNT          983 a          556 c          708 g          698 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agttaccgga tatcggtgaa
      241 ggaatcgcag aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa
      301 gatgatacat tactagaagt tcaaaatgac aaatcagtag aagaaattcc gtcacctgtc
      361 acagggacag ttaaaaacgt gattgttcca gaaggcactg tagcaaatgt tggggatgta
      421 ttagtagaaa tcgatgctcc tggacacaat agtgcaccgt caactagtgc gccatcagct
      481 gaagcaccaa aagaaaaagt ggaaacatca ggttcagcaa gtgtcgttga agctgccgat
      541 ccaaataaac gtgtattggc tatgccatct gttcgtcaat ttgcccgtga aaaagatgta
      601 gatatctcac aagtaactgc aactggaaaa ggtggcagag taacaaaaga ggacatcgaa
      661 aacttcttgg caggtggtcc aagcagtgca ccagcgaaat cagaagcgcc agaagcggca
      721 gcaccgaaag aagcagctcc tgcagcagaa agcaaaccag ctgcaccagc aaaaccattc
      781 aagtccaact taggagactt ggaagaacgt gtagcaatga cgccaacacg taaagctatc
      841 gctaaagcaa tggtcaacag taagcataca gctccacacg tgacattgca tgatgaagta
      901 gaagtgtcta aactatggga taatcggaaa cgatttaaag aagttgctgc tgcaaatggt
      961 acgaaactta ctttcttgcc atatgtcgtg aaagctttaa cagcgactgt taagaaatac
     1021 ccagtattga atgcatctat cgacgatgca aaccaagaaa tcgtttataa acattactac
     1081 aatatcggta ttgcaactga tacggatcac ggactatatg taccaaatgt gaaagatgca
     1141 gaccgcaaag gaatgtttgc tattgcagac gaaatcaatg agaaagcaaa attagctcat
     1201 gacggcaaac tatctgcaga agatatgcgc aatggaacaa ttacgatcag caatatcgga
     1261 tctgtaggtg gcggttggtt tacaccagtc atcaactatc cagaagtagc tattttaggt
     1321 gtaggaacga ttgcacaaca accaatcgta aatgctgaag gcgaaatcgt tgtcggtcgt
     1381 gtgatgaaac tgtcattaag tttcgatcac cgtatcgtag atggagcaac tgcgcaacaa
     1441 gcaatgaata atatcaaacg tcttttagct gatccagagc tattaatgat ggaaggatga
     1501 taaacaaatg gtagttggag attttgctgt agaattagat acagttgtaa ttggtgcggg
     1561 acctggtggc tatgtagctg ccatccgcgc tgctgaaatg ggacaaaaag tagcaatcat
     1621 tgaaagagag tatattggcg gtgtttgttt gaatgtcggc tgtattcctt caaaagcact
     1681 gatagctgca ggacaccatt atcaagagtc actagattct gaaatgtttg gagtaactag
     1741 tgaaaacgtc aaactagatt ttgcaaaaac acaagaatgg aaagaaaaca aagttgtcca
     1801 tacgttgact tctggtgttg gtttcctatt aaaaaaacat aaggttgaaa caatcgaagg
     1861 agaagcgttc ttcgtagatg atcacacact acgcgtcatt catccagatt cagctcaaac
     1921 ttattcgttc aataacgcaa tcatcgcaac aggttcccgt ccaatcgaga ttccaggatt
     1981 taaattcgga ggacgtgtgt tagattctac tggcggatta gcattgaaag aagtacctaa
     2041 gaaatttgtg atcatcggtg gcggtgtgat tggtgccgaa ttgggcggag cgtacgcaaa
     2101 cttaggagca gaagtaacga ttcttgaagg ctcaccacaa attttaccaa cttacgaaaa
     2161 agatctagta aaattagtag aagatgattt caagaaaaaa ggcgtaacag tcgtaacgaa
     2221 tgcaatggcg aaagaagcag tcgataacgg tgacagcgtg acagtgaaat atgcagtgga
     2281 cgggaaagaa gaatccgtta ctgctgatta tgtgatggtc actgtaggtc gtcgtccgaa
     2341 tacagatgat atgggcttag aacaagcggg tgtcgaagta ggagaacgag gattgatcac
     2401 ggtggataaa caaggacgta cgaacgtacc taacatctat gccatcggtg atatcgttcc
     2461 aggggcggca ttagcacaca aagccagtta tgaagcaaaa atcgctgctg aagcaatctc
     2521 tggcaaaaaa gtagctgtag attataaagc aatgccggca gtagccttca ctgatcctga
     2581 attggcttct gtaggtatga cgatcaaaga agcaaaagat gctggtatcg aagcaaaagc
     2641 atataaattc ccattttcag ggaacggtcg ggcattatcg cttggtaaaa cagaaggatt
     2701 catccgtcta gttacgacga tcgaagataa cgtgcttatt ggcgcgcaaa tcggtggagt
     2761 cggcgcaagt gatatggtct ctgaattagc tttagcaatc gaatcaggaa tgaatgctga
     2821 agatatcgcc ttgacgattc atcctcatcc atctcttggc gaaatcgtca tggatgcttc
     2881 ggaattagca ttaggtttac caatccatat ttgatttgaa taatacaggc gaaacgcaac
     2941 tgaaa
//
LOCUS       DACDYJ010000144         2921 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDYJ010000144 DACDYJ010000000
VERSION     DACDYJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.148"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1364..1882)
                     /locus_tag="IWM51_002786"
     CDS             complement(1364..1882)
                     /locus_tag="IWM51_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP9972122.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          969 a          529 c          412 g         1011 t
ORIGIN      
        1 ttcttttacg ttctattcag attaacgggt aggcttactg tcttttttgg caataatttt
       61 gcaatcaagc catttacaga tgtaatgcgc ggatacttct gctaagattg caaaaaacat
      121 atcgaaaagg taattcataa tacaacctcc tccccttgcc ataataggcc ggggtagcag
      181 acagcataaa tattatacca taactgaaaa tttcatttta gtacatttca atatattgat
      241 gaaccagcac aataaaacat gccatgacta acagaaaaaa acttattaaa ccagatataa
      301 cttgtaaaca tccaactata tagaaaacgt aaattttcta tatagttgga ttaagataac
      361 aactatttat tttatcatat ttttactata ccctatactt ttacttggta tatttatgaa
      421 taatcgcaaa tttaccccta tactactagt ttgtaagtta gattaataaa aatactaaag
      481 ttatttgttc ttttattaaa tacacctaca acttataaat tgatggtatt tagataacac
      541 gaaagaataa aaataacagc tgatagtaaa gtttgttgac gattgctact caatcgaaag
      601 tgagttagtg cgtatgaact tgcttctgaa aacttatgaa atgaaagacc ttttacatct
      661 tgcagaaatc ggtactggga ttaaactaag caataaaaac taatgttccc ctcattcttt
      721 gaacttctac tatttgattt atttattctg ataaatgacg atttcacctt caggatcttc
      781 aaatcccgta tagaatttat ttaagatctc ttcagtatca gtaataggat aggcgatagg
      841 ccacataccc tttgatggtt agaataaact gctcaaaatc tgtatcgtat gttggcttta
      901 gcatattaac aaaggctatc tccaaattat aaaccataag cgttgaatcg ttcttaacaa
      961 tttcaattct tcctaaccca aagtcagaag atataattac cggacttgcc ttacccttca
     1021 tgtgggttgt tgagattcca tatttaaagg gaattgtcgg tactatcggc ggtctgaaaa
     1081 acatcccaaa aataaaaaaa tctttcccct gctagtctcc taagtccctt tgtagcggat
     1141 atggcaccat aatctcattt tatcgaagtg agtcatggtt tatctgttgg ccaaccaaaa
     1201 ttgatacaat ctaacgatgc accctctacg gcattctagg ggagtgcact tgcattcacc
     1261 caataaaaac cggcagttag caaagaactt atgtagtcgt atgttcttac tgttaacata
     1321 caagaggtct cctttccaca attgcttagc agtaaacggg aatttacaaa gctaatctct
     1381 tccattttct tatctttgtt ctaaaagtcc caaatggagc aactgtattt acatgaataa
     1441 atttatatac ttcccataca gccgttttgg ttgcctcgtc tgcccatttt cttttgtgtg
     1501 cagtgaatag atcttcatca ctcattgaat caatcatcat gttaatatca tcaatattac
     1561 gattgagaat ttcctctaat tctttcaatg gtagatgtgc ataggtattt gtaaaccact
     1621 tatataactc tcctaactga ttccatttaa attgatcaga aggcgtttta acagataatc
     1681 catttctttc atcattttcc cattttagaa ttaaattcgt ccaaccaact tgatatgcta
     1741 aattttcagc aggtgttcta tccacttcct caactctttt atccttcaat tcttcaggaa
     1801 tagtttcaaa ttctaaaata tatttagtat aagtcctatt tatttctttt tttaattctt
     1861 ctttattttt atatgttctc attatattac ctccataaat tcctaattgt ctcctttaat
     1921 tcatctataa tttcatccac gatattttaa tccgttctac aatgcatatc tgatatgcat
     1981 tgactgtcta actcatatgt gaatgattta taaaatatcc caacgtctca tctaaagaaa
     2041 tgttgatcgt cagcaaggag ctaaaaaata cgaatatatt tcttttgtag taaaatcgga
     2101 ctggtttgca tatcattttc ttcgtaaata tcaaaatcat gttaactact atcaattact
     2161 aataaaaact accactttaa gcgttaagaa ctgttaagtt tagttaagtg aggatagttc
     2221 acaaaatcca ttgttaaact tcacgaaagt ggtaaaactc aaaatgcttt atataggatt
     2281 acagcatcgc tctttcttcg attgcgcgtt gggtaaaaaa tattatgaag taaaaattga
     2341 cgatgacacg ttccttacgg ctcatcagat gaaacagcta caaaaacgaa atgatcaact
     2401 cgaagaaaat ttaattctaa aaaagcgatt gccatattca cgcctcgctc gacgaactat
     2461 taaaggtcaa gatttacctg aagatcatcc tttttaggaa attgaagctt tttatgttgc
     2521 acaatgcatt tataatacta caatgatgtt ttcaccagat attcttctag ggtgcggtgt
     2581 aatgaaacag atacacttgc aaaagaaaat tcaccgcgaa tttactaaaa ttatgaataa
     2641 ttatgttaat cttccgaaat ttttttccaa aattaaggga tcaagctggt gttatagatt
     2701 gtttaaggtt ggcaaaaaaa taacttcttt ttaaacaatt atagaaaacc gatactctgt
     2761 taagttggtt gacacggtgt cgatttttat gactttctta ttctgaaact tcactaacgg
     2821 acagtaccta aaaagtctat agtatggacg tttacgagta ctattttatc aaaaatgctg
     2881 ctcatttcaa cttatatgtt ttagtgaact acccccactt a
//
LOCUS       DACDYJ010000145         2857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDYJ010000145 DACDYJ010000000
VERSION     DACDYJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.149"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            562..843
                     /locus_tag="IWM51_002787"
     CDS             562..843
                     /locus_tag="IWM51_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972123.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLEKFIAIQTKNTQVLNDYVHTNNQLIETLIGGK"
     gene            845..1261
                     /locus_tag="IWM51_002788"
     CDS             845..1261
                     /locus_tag="IWM51_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972124.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTDVLIGTNSKVYQEVKEKT
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1422..1688)
                     /locus_tag="IWM51_002789"
     CDS             complement(1422..1688)
                     /locus_tag="IWM51_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972125.1"
                     /translation="MAFRFRCDRGGRKMTEKFPYEYYQNLEKLESAIQLVKLDLLDEN
                     LLKYKNITPERKGKLKKMQQLFDEEDGISDVIFDTWSEFHGEYN"
     gene            complement(1646..2620)
                     /locus_tag="IWM51_002790"
     CDS             complement(1646..2620)
                     /locus_tag="IWM51_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9972126.1"
                     /translation="MGKKRTIVNEWEKLTAPSNRRLNVGESVKMIKMDWSIDRITIVG
                     TLNENIYYHTQNDVLILDFEQLMRVNEGNGHLKAVGNNGWQLVDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKKFIHDLFLEPHFSRADVACDLIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQEAKRKIVPKEVETWWRLELQ
                     LRRGKATDWYEMVQESLDSFASPHYFPEDVKTLDRVMIKGLLYDHSEWSTISRDLKYR
                     LRKLLKQESQNDELTNHLRETFEESADELKRELDTWLLGLDVTEEEEK"
BASE COUNT          927 a          624 c          396 g          910 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatcattt cacgcttccg ctcttcattt cattcagttc tcggctttca caatctaaca
      121 cgccgtttcg ctaccagtcg ccttacaggc tccattctcg cttctcttac gtgttttacc
      181 gattgtgccg acaactaaac gaaatgaacg ctaaaacgtc ttagaacgca aaatacacgc
      241 ttataaaatc atttcatctt gattatatgc tcatcataat cgttcaattt gacatcattt
      301 ccgtaaactt tttttatttt tataagcaga aaaaagtcgc acatcaacat ttttttaaag
      361 agcataagga aacgtgtacc cactttctaa aaaaagcggg cagataggct gtgcctatca
      421 cgtttctaag aaaaaaattt gatatcactt tttcgccatt gtgactagct tatgtatagt
      481 gtttcagcaa aaaacagcta tacaaatact atattcgtat gatatacata aattatacac
      541 aaatgcgaaa ggagtttttc tatggacatt ttagttcgga agattgattc aaaggttatt
      601 gcaaaaatag acaaacgttg ccaagaactc acaaataaaa ctggcaaaaa atggagtcga
      661 aataaatatc taaaagtttt aatcgaaagt gattttgacc gtcaacttac gacttacaaa
      721 aaagaccaat tcgatttatt actcgaaaaa tttatcgcta ttcagacaaa aaacactcaa
      781 gtactaaacg actatgttca tacaaataat caacttattg aaacacttat tggaggaaaa
      841 tagcatgaga aaaacttctg ttcaaaaaca cgttaccctt tatcccagtc acgtggacgt
      901 tttagattcc attatcgaaa gaaatccaga tatcaacaat tactcggaag ccattcgttt
      961 tttatgtatg agttatgaac ctgataccaa cacgactact accagcaatc aagccaaact
     1021 aaatgctatg ggaaaagaaa tttccatcat gtccgaaatc attgcagaat ttgctgattt
     1081 atatctaaaa gatacagacg ttcttattgg cacaaattct aaagtgtatc aagaagtgaa
     1141 agaaaaaacc gaagctaaac taaatccgtc gaatcgaaat cgaccaaaga atcatcaaaa
     1201 agaaacgagt caaacagtca cttctagaaa tttcgaccca acaaattttc taaaacgcta
     1261 aaaaataaaa cgagaatttt tttatttctc gttttatttt ttaggaaagc cggtcttaca
     1321 gcaagatttg caagttaccg gtaacttgcg gaaaagaaga agccgctcac gcgacttctt
     1381 ctttttgcat tttctgaaag ctaccacaat tcctaacttc gctaattata ttctccatga
     1441 aattcgctcc atgtatcaaa aatcacatct gaaattccat cttcttcatc aaataattgc
     1501 tgcatttttt tcaatttacc ctttctctct ggcgtaatat ttttgtattt tagtaaattc
     1561 tcatccaaca aatccagctt gaccaactga atagcactct ccaatttttc tagattttga
     1621 taatactcgt atggaaattt ctcagtcatt tttcttcctc ctctgtcaca tctaaaccta
     1681 aaagccatgt atccaattct ctttttaatt cgtctgcaga ctcctcaaat gtttctcgta
     1741 aatgattcgt caattcatca ttttgacttt cttgcttcag caatttcctt agccgatatt
     1801 tcaagtcacg actaatagtt gaccactcag aatggtcata caacaacccc tttatcatca
     1861 ctctatccaa cgtttttaca tcttcaggaa aataatgcgg actagcaaaa ctatccaaac
     1921 tttcttgaac catctcatac caatcagtcg cttttccacg gcgcaattgt aactccaaac
     1981 gccaccacgt ctcgacttct ttaggtacaa tttttcgctt tgcttcctgt tccaacttct
     2041 tgttgtacat ccgaatttgg cgttcgctgg cacgactacc ccaataagcc gtttctagct
     2101 taccagagcg tccataaatt ggtttgaagg acacagggtc aactacacga tattgtgtaa
     2161 taaactcatc aggcacatca ataagatcgc aggcaacatc cgctcgtgaa aaatggggct
     2221 ctaaaaataa atcatgaata aattttttca tagagcttgc taaaaattga ctaattttat
     2281 tggggttaaa gtcaatccgc cctttaccct cttgccattt caaaatttcg atataggcaa
     2341 tattttcttc gtgttgatct accaattgcc aaccattatt cccaacagct tttaaatgac
     2401 cattaccttc gttcactcgc attaattgct caaaatctaa aatcaacaca tcattttgag
     2461 tgtgataata aatattttca ttcaaagttc cgacaatcgt aattcggtca atcgaccaat
     2521 ccatttttat catttttacg ctctcaccga catttaaccg cctgttagag ggggctgtta
     2581 atttttccca ctcattcaca attgttcttt ttttacccaa attttgttcc tcctcaaaga
     2641 aaaaaagaga tttttggaaa gaatttccag agttcacgta actctaaaaa tccacatcca
     2701 aaaatctcta gcttgaagaa acctaaaaat attcgtataa tgaacttgag ttacggaact
     2761 aggctagtag tttccgtaaa ggtcgagagt gttagcgcac tgctcgacta tttttttgct
     2821 cttttttctc gttttctttg attatagcac tcctctt
//
LOCUS       DACDYJ010000146         2821 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDYJ010000146 DACDYJ010000000
VERSION     DACDYJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.150"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            95..1054
                     /locus_tag="IWM51_002791"
     CDS             95..1054
                     /locus_tag="IWM51_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1252 family transposase"
                     /protein_id="HAP9972127.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINF
                     LKEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPIS
                     CSVRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEF
                     GHIEGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFK
                     SITFDCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQ
                     VDQAFISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
     gene            complement(1487..2059)
                     /locus_tag="IWM51_002792"
     CDS             complement(1487..2059)
                     /locus_tag="IWM51_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9972128.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2075..2680)
                     /locus_tag="IWM51_002793"
     CDS             complement(2075..2680)
                     /locus_tag="IWM51_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP9972129.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          888 a          579 c          469 g          885 t
ORIGIN      
        1 gccgattgta aaattaagct agacaaataa ataagccatt cgtgctacac tcaaaatgta
       61 attccgacta aagaaaacat aggagtgatc acgaatgacc tacacccatc ttaccacgga
      121 tgaactggtg atgatagagt cttactacca ccaaaacatc aaaattgtga aaattgctga
      181 gtacctgaaa cgatctcgaa caccgattta taacgtcatt aatttcttga aagaaggcca
      241 tacggccctc gaatactacc aacagtataa agaaaataag aaacggtgtg gcagacgaaa
      301 catcgtcttg ccaaagaaac aacagtccta cattaaagaa aaaattgccc aaggctggac
      361 gccagacgtt atcattgggc gtaaagagaa accaatcagc tgttccgtaa gaacccttta
      421 tcgccagttc aaggaaaagg tgttcgatga atcgactttg cccatgaagg gtaagcggaa
      481 accaaatgga catcaagaac gccgaggaaa acaggctttt aaacggaata tcgttgaaag
      541 agaaactgat tacccgacat ttaaagaaga gttcggccat atcgaaggcg ataccattgt
      601 aggcattcat cataagagtg ctgtcataac tttggtagaa cgtctatcga aaatcatcat
      661 caccttgaag cccgaggggc gcaaggcgat cgatattgag aatactctga accactggtt
      721 tggatcgatt cccagacatt tatttaaatc cattaccttt gattgtggga aagaattctc
      781 taattggaag gctttaagta accaacatga catcgctatt tatttcgctg atccagggac
      841 tccttcgcaa cgagcactga atgaaaattc caatggcttg ctgcgaaaag atggtctgcc
      901 aaaaaacatg gattttaatc aagtcgacca agcttttatc tcttcagtgg cgaataagag
      961 aaacaatatt ccacgaaagt ccttggatta tcaaacaccc ctggaagtat ttatgagtta
     1021 catgaatgaa gatattttgt ctagcttaat ttgacaaatc aaattattta aaactttgca
     1081 acagaacctt agcagcagtg ctgattttta tgatagttat ttggttctta aatgaaagat
     1141 ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca atagattatt
     1201 tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg aacgatatgt
     1261 gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc taatatagta
     1321 agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca cattttttga
     1381 gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag actcatatct
     1441 agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa ctttcttcaa
     1501 ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct tcaacttcct
     1561 tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt gtgattgttg
     1621 ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt tttccttctt
     1681 gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac gtccgtgtca
     1741 ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg atcgttacgt
     1801 tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga gcaaatcgat
     1861 caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc tgattgaatt
     1921 ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc ttctctacgc
     1981 cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt gagacacgtg
     2041 catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc gtatactcag
     2101 aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg cgatcgtcaa
     2161 ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt atgggacttc
     2221 gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca actttttgca
     2281 attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt ccttctcgaa
     2341 atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa gaagtttgtg
     2401 gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca aatcgaaaat
     2461 ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata tccgaaaata
     2521 gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca tttatttttc
     2581 cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc ttagccaact
     2641 caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc cttaacttta
     2701 tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt tatgtgtcaa
     2761 aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt gcacagtcta
     2821 g
//
LOCUS       DACDYJ010000147         2726 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDYJ010000147 DACDYJ010000000
VERSION     DACDYJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.151"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..221
                     /locus_tag="IWM51_002794"
     CDS             <1..221
                     /locus_tag="IWM51_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9972130.1"
                     /translation="RSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKG
                     NKIAKLEFTFSSLTSDLPKISLRNWLEE"
     gene            complement(358..774)
                     /locus_tag="IWM51_002795"
     CDS             complement(358..774)
                     /locus_tag="IWM51_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972131.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(777..1061)
                     /locus_tag="IWM51_002796"
     CDS             complement(777..1061)
                     /locus_tag="IWM51_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972132.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(1761..1856)
                     /locus_tag="IWM51_002797"
     CDS             complement(1761..1856)
                     /locus_tag="IWM51_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9972133.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            2487..>2726
                     /locus_tag="IWM51_002798"
     CDS             2487..>2726
                     /locus_tag="IWM51_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9972134.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLN"
BASE COUNT          878 a          434 c          515 g          899 t
ORIGIN      
        1 ttaggagctt aatggatatc ccagaatact atcaaatggg taacattgat caaaaagtat
       61 taaatcctgc attacgagaa ctaaaaaaat acttcaatga tcttaaagtc acaaaaataa
      121 aagcaaaaaa gggcaataaa atcgccaaat tagaatttac tttttcaagc ctaacgtcag
      181 atttgccaaa gatttctctg cgtaactggt tagaagaata aataaaaaag aactcgcccc
      241 ccttacatta agcaagtaga caagtccctt acaaccgact aagattgcta gtacattcta
      301 gcatattgta tggggcttct aaataaaaaa cagataggaa taactcctat ctgtttttta
      361 tcgttttaga aaatttgttg ggtcgaaatt tctagaagtg actgtttgac tggtttcttt
      421 ttgatgattc tttggtcgat ttcgatttga tggatttagt ttagcttcga ttttttcttt
      481 cacttcttga tatactttag aatttgtgcc aataagaacg cctgtatctt ttagatataa
      541 atcagcaaat tctgcgatga tttcagtcat gatggaaatt tcttttccca tagcatttag
      601 ttttgcctga ttgctggtag tggtcgtgtt ggtatcgggt tcataactca tacataaaaa
      661 gcgaatggct tccgagtagt tattgatatc tggatttttt tcgatgatgg aatctaaaac
      721 gtcaacatga ctagggtaca aagtaacatg tttttggaca gaagtttttc tcatggttat
      781 atcattcctc cgataagcat ttcaatgatt tgattatttg tgtagacata ctcgcttaat
      841 atttgagtat tttctgtttg gatagcaata aatttatcga gcaataaatc gaattggtct
      901 tttttgtaag tcgtaagttg acggtcaaaa tcactttcga ttaaaacttt tagatattta
      961 tttcgactcc atttttctcc tgttttattc gtgagttctt ggcaacgttt gtctattgtt
     1021 gcaacaaact ttggatcaat attccgaact aaaatgtcca taaaaaatct ccttttgcaa
     1081 ttatgtataa tttatgtata ccatacaaat actatatttg tatagctgtt ttttgccaaa
     1141 atgctataca taagctaccc acgatggcga aaaagtgata tcaaattttt ttctgagaaa
     1201 cgtgataggc acagcctatc tacccacttt ttttagaaag tgggtacacg tttccttatg
     1261 ctctttaaaa ttcggggggg agtgctgcac gtgtaatcga taaccaaatt taacagacga
     1321 aaaattgttg gaatataaaa atatgacacc cgaaagaaaa cgatcgttgg aaaaattaat
     1381 gcaactagtg aacgaagacg gcggaatttc agacgcagct tttgacgcat ggagcgaatt
     1441 tcatggagaa tataattaag tgggtttaaa tggctctaaa atcgcttcta agagtttttt
     1501 agcttggacc aatgaaatac accaaaaact aaataaaacg tcttaaatcg aaaaatacga
     1561 gctctcagag cgactttatt tgtaaatgtt gcatataaga aataattacg gattttagac
     1621 ttttgtagga tatttttgaa ataaatattt atttctgtta taatttgatt gcaaataagt
     1681 gaacacgaaa cacacgaaat ccccagagag gtcggattct ctggggcttt ttttgtgggc
     1741 tgagtcgcct taattaagtt ttacttcttg tttcggttat tcaaccagta actgaacaaa
     1801 gcgcttaaaa gcccaacaag aagggagcaa ataaagttag tgaacacgaa acacacctcc
     1861 ttccagtcgc aagactgtcg gataggcgac aatcaattat acaataaaaa agcaaaaata
     1921 agcctaagtt ttttattgcc ttggggttgt tagggcttgc cctgaccgcc tgtaaggcgc
     1981 tttagaacga aggacatagt ttttatcgaa tcttgtctta gaacggctta gacgagctct
     2041 caggacgcca caaaggagtc cctaatgaat tatttttggc gaaggagcct aaaggcgact
     2101 gctgcaagta aaatgtgaga gcgtagcgat cactccttct tacagcgacc atagggagct
     2161 agattggcag tttaatatca tgcgcgaacc tgtttatttt aaaaagtggg tgattttttg
     2221 gcgggaaatt ttgctcgcgc gcgcgcgttt attttattta ttttttaaag attttttaga
     2281 acccttttta gggggcagtt gagccttact ctcccaacgg atacagaagg gtactaacaa
     2341 ctttttatct gagtactaac aactttttat ctgagtacta acaacttttt atctgagtac
     2401 taatatcctt ttgtataata acaaccaacc tatttacgag ttggttgtta aaggttatta
     2461 tttatttgta aggggggaag aaaggaatgg atgaaaaaac ggaattaaca gtcaaatatc
     2521 aaaatgaatt gaatttagta ccgctgaaaa atttcaatgc taagcagatg gatttatttt
     2581 ttgcattatg cgctcgtatg aaagatcgtg gtttgaaaaa agttagattt tctttcgagg
     2641 aattaaaaga gttaagtgat tataagatga ctgctacaaa agcctttgtc ggtgatttag
     2701 aaaagctcta taaagatatg cttaat
//
LOCUS       DACDYJ010000148         2493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDYJ010000148 DACDYJ010000000
VERSION     DACDYJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.152"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..821)
                     /locus_tag="IWM51_002799"
     CDS             complement(<1..821)
                     /locus_tag="IWM51_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP9972135.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEEL"
     gene            complement(1170..1496)
                     /locus_tag="IWM51_002800"
     CDS             complement(1170..1496)
                     /locus_tag="IWM51_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972136.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(1483..2271)
                     /locus_tag="IWM51_002801"
     CDS             complement(1483..2271)
                     /locus_tag="IWM51_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9972137.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT          719 a          411 c          359 g         1004 t
ORIGIN      
        1 agttcttcat aaacgacaat agtttgctct aattcttcag cttttttctt tgcattatga
       61 atagtttgga cggtttgttg agcttcactg taacttgaag aaaatgtttt tatcaagttt
      121 aatacatttt ctggtacaaa tgtatttata ctgtcctctt gcatgatgtc gacgaagtta
      181 tctagcaaaa cttgattttg taactggtca gtttcagtgt ctattatgtg tctattagtg
      241 tctatagaat tagaatagaa tagattgtct gcgattttcg caattacggt tgagtccttg
      301 atatgactgg attttgccgt atttgcgatt ttcgcaatta cggtagaatc attgattttt
      361 cgcggttttg ccgtatttgc gattttcgca attacggtgg aatcagtatt gtcttcctct
      421 tccatgattt taaagacgtc ttgattcgta acttctggct tgcctaagta caataaatta
      481 ggcatagatt caatcttacc atcacgttta tttactcttc ctctcttctg tttcagaaga
      541 cctgcttttt ctaattcttt tttgatctta gatactttac cttcgcggca ttgtaacaat
      601 ttcattaatt cttcaactgt gaatatgaag aatatattat catcagaatc tacccagtta
      661 tttttgactg aataatcaaa tcgatcttta agtagcatat aagcaatttt tgcatcattt
      721 gaaagatcca tgtatttttc gttagtaaaa aataccttag gcatttgata atatttttca
      781 cgataaattt cttttgattt gtaataatta aaatcattca tagccaatcc tctttctgta
      841 ggatagcgtt ttgttcaaaa ctatgttaca atgaattaaa caaatatatc tatccactat
      901 atatttttct actgctgata agtcgccaaa ctttgttaaa gcagtagtct ttttttatct
      961 ttttttagtt taccttaaaa aataaattaa agcaaaccgc tctatttcag ggtttaagct
     1021 catggtaact atatcacgat attacaataa cattatatca cgatatcgta atattacaat
     1081 atgaagataa gcgacttatc agctttgata ctactgattt ttataaaaaa aatatcacga
     1141 tattatgata tcgtgatatt gaatgtaaat taaattttat tttgtttctt tgctagttta
     1201 tatccttcaa tcaagtattc ttcaggtatg gattttaata gcgcatcttc tataacgtct
     1261 ggtttacttt tgtctgattc agcaatagtc gtccaatatt tgagtgctga cattagaatt
     1321 ggatttatac gaatgctatc tgctgttctt acactacttc cttgatcttt tatgacttta
     1381 cttatatttt tcttggttaa ttcgatttct tctacttctt ttggtttgta gttcttttta
     1441 tttttatttc ctagtagtct acttactgca gcttgactgc gattatcttc tgccatcaaa
     1501 atcaccaact tcttttaaat attcttctgt aaatgcttta tagtcatctt cagctgttga
     1561 gggtttatta aaattgatta gatctgatcc agcagcttgc gcttcgtcaa cggttattgt
     1621 tctacgaata taagttttga aaatttttgc accaatgact ctagaaattt cggttgctgc
     1681 agtcgtcatt tctttagaaa tgttataacg cttattatac ttagtaaata ggactcctcg
     1741 tacttcaagc ttgtcgttcg tatattcttt taccgtttca atagtttcac ttaactttat
     1801 tagtcctttt aatgagaaat acgcagcttc aactggaatg ataatttcat cagaaacagt
     1861 aaatgcgttg actgtaagca ttcctaaact tggtggacaa tcaattataa tataatcata
     1921 gttgtccttg attggttgaa gtactctttt aagcctgtgt tcacttccta cttgagtaaa
     1981 ttccctctct gcagaagaaa gggctatgtc ggctggcaaa acatcaattc ctttatagtc
     2041 cagaatagca tctgtaatgt cagcctcccc ttttaatact tcataaattg taggttggtt
     2101 tgttccatct atatcgaaac ctacattatc tgtagcattt ccctgaggat caatgtccac
     2161 taaaagtaca tttttaccgt aaacaaattt caaacatgct gctaaattaa cgctagtcgt
     2221 agttttagaa actccacctt tttgattggc tactgacaat attcttgcca ttaaaatatc
     2281 ccccattcat cttatattat aatataatta tatcataata ttgtgatatt gcaatatcgt
     2341 atatgcatta tattgtaata ttgtgtgatt attttgtttt aaagatatat atcggttcgg
     2401 atataaagat tattcatatg ttattcgaaa gtaaaataat cactttttat tactttaaaa
     2461 ttttttatta atcataagaa atatgttttg cga
//
LOCUS       DACDYJ010000149         2484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDYJ010000149 DACDYJ010000000
VERSION     DACDYJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.153"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1014..1304)
                     /locus_tag="IWM51_002802"
     CDS             complement(1014..1304)
                     /locus_tag="IWM51_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972138.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2204)
                     /locus_tag="IWM51_002803"
     CDS             complement(1512..2204)
                     /locus_tag="IWM51_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9972139.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          734 a          458 c          349 g          943 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgagtttc tacatttgtt
     1681 ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct
     1741 gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt
     1801 tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt
     1861 agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga
     1921 tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa
     1981 tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct
     2041 ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc
     2101 tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat
     2161 agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa
     2221 tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa
     2281 ttacatatgt aattttaata taaatactat caaatactca tttaatcctc cttttttatc
     2341 tatttaaaaa atctttttta tagagaaaaa tgcaggttaa tactttctta aataaatatt
     2401 tatggtgaac aaaaacaaaa aagcaagttt tattaaacgt aacccttttc atttcataga
     2461 ttactgtatt tattacaaaa aacg
//
LOCUS       DACDYJ010000150         2455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDYJ010000150 DACDYJ010000000
VERSION     DACDYJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.154"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            132..344
                     /locus_tag="IWM51_002804"
     CDS             132..344
                     /locus_tag="IWM51_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972140.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="IWM51_002805"
     CDS             506..1024
                     /locus_tag="IWM51_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAP9972141.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="IWM51_002806"
     CDS             1031..1543
                     /locus_tag="IWM51_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972142.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1814..2437
                     /locus_tag="IWM51_002807"
     CDS             1814..2437
                     /locus_tag="IWM51_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9972143.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          944 a          310 c          409 g          792 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agttttaaaa
     1561 taaactaatg tccttatttt tgtgttcaca tttctgttac ctaagaaata cttaaaggag
     1621 gtgatgtagc cagcaacgac tacaacttga acccgctatc tacagtttat ttgttgtttc
     1681 ttttgggtat actctaatgg tacaaatcca tatttgctta aagcgttgat ttttcagcgg
     1741 tttttcttta ttgcaaagtg taccctaaga cctgtatact aatagtaaat agattggata
     1801 ggaatgatgg caaatgtatt tagcttattt aagagtttct tccgacgatc aaagtttagc
     1861 tcggcaacat aagctaattg aaaattggtc agtcaaaaat aatattgctg aagacgattt
     1921 aaaaattttc gaggaaaaag tctctggaaa aaatattgaa aacagaccag agctgcaaga
     1981 gcttatttct ttcatacgtg aacgagatac gattattatt cctagtcttg atcgattagc
     2041 aagaaatagt aaagatacaa aagacttact tcaattgttt cgtagtaaag gtgcttctat
     2101 tgaaatatta gacctaccgt cgtttaatgg ggtaactgac cctgcattaa gagacttgct
     2161 aacaaattta gtcatagaag tgtttagcta tgttgctgaa aacgaacgga aaaaaatcaa
     2221 agagcgtcaa aaacaaggca ttgaaattgc aaagaaaaat ggtcgttata ctggtaaaaa
     2281 gattcaatac catgccaatg caacaggaaa aaacaaattg atttacgatg aaattgttga
     2341 tggtctgaag aaaaaagaaa ctatttctaa aatagcaaag aacgcaggag ttacaagaaa
     2401 aaccgtttat cgagtaaaaa gagatttagg aatgtaatat gttccagtta gaaag
//
LOCUS       DACDYJ010000151         2447 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDYJ010000151 DACDYJ010000000
VERSION     DACDYJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.155"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(375..692)
                     /locus_tag="IWM51_002808"
     CDS             complement(375..692)
                     /locus_tag="IWM51_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9972144.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTIHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQIK
                     LGF"
     gene            complement(693..947)
                     /locus_tag="IWM51_002809"
     CDS             complement(693..947)
                     /locus_tag="IWM51_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9972145.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1207..1425)
                     /locus_tag="IWM51_002810"
                     /pseudo
     CDS             complement(1207..1425)
                     /locus_tag="IWM51_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          801 a          444 c          352 g          850 t
ORIGIN      
        1 taaaacacaa aaataaatac taccttattt aggtagtatt tattaaaagt tacgtcaatt
       61 ttgattaaaa atatactact tgaacctctc atgagtaaag tcacgaaatt tttgggaata
      121 gagcgtactt gatatcttat tttttcacta atagcagtgt ctcttattca ccaagcgatc
      181 cccgtagttc ctacggttct taatttttct ctagaccaat gattgtatct ttagaccttc
      241 ggccataaca tggagcaaaa taaaaaggta ctaagtctta tatatcaatc ttcgagtaac
      301 cgacggttaa caaccatatc gagcggcatt aatcacccac ctgtagagga tgagcgactt
      361 ttgcctaatt attgttaaaa gcctagtttt atttgtttag attggaaaat acgtcctaca
      421 agtatctctt ttttttcatg gtcgatccga taaaataagg catagctttt gccgatcacg
      481 atccgatatg ttgggatatc cattccatag atttcggaaa cttcctcatg tatagttgga
      541 aaatttttgg ttagttctaa agccttctgg atcgcaccta caaatctttt tatgctggct
      601 ggagaaagag caagctcatt ttcccattct tctattatcg tttcaaggct ggtctggaaa
      661 cttttagaat aaactagcgt gtactctttc atctattcac tccagccgta ttttccgaaa
      721 gcttttacca tttcaatttc tgaaactgtt aaacctttct caaagtcttg gaaactttct
      781 tcaatcaatt tgcgatcgtg ttcttttgct atttgagtag tatctaatat gaataagttg
      841 ccttcttgtt tggcttggat agtgtctccg ctttttaatc caagagagct tggaaggatc
      901 actcccacgc tattgccgat ctttctaacc tttagttctt ttcccatgtt atcacactcc
      961 ttttagtttt tgatgaataa attataacat gttataacaa caattgccaa cggtgtttct
     1021 taagggattc cactccatat tattattgga caaaataagc cacttagaac gctgaaaaca
     1081 tatacaaacg atatcaaaat acgagtgaac tactcaccat acacttccgt taacatccat
     1141 ttaagtaggt atttttatta tatatagatt taacatatgt cggtcggttg attgatatag
     1201 ctaaagttat aactcaaccg aagccgattc atctacacga ataaagttgc gagtgttctc
     1261 ggctaattca taaaaattgt tatgtgttcc atatagcaat actggaataa ctttattttc
     1321 ttgaatctca taagcaactc tatacacggt gaccgcatac ttaaatccat tagaccaatt
     1381 acctgataaa cctccagttt tttgctctta cttaaatgga tcaatagcaa tttcatcaca
     1441 aacctataga ggatgagtga cttctgccta attacgttaa aaaatattga aagtaactct
     1501 atgtagcaaa aaatatctag aatttcattt cctcaaaact gatcgatatt gtatctaaaa
     1561 atatatacta cctaaaatag gtagtatata ttaaaatatg tgcttttgtt agaaaaatca
     1621 atacataaat tatcatatct gtatatgtca tttaggtatt tcagttatgt tataatagtt
     1681 ttgctgtctg ctaccccggc ctaatctggc aaggggagga ggagatataa tgaaatacct
     1741 ttttgatttt gtttttgcaa tcaagccact tgcagatgta gtgtgcagtt acctctgcaa
     1801 atggcttgat tgcaaattgc caaaaaagac agtaagccta cccgttaatt ctataatata
     1861 gaaacgtaaa aaaaacccca tcatcctgca cgctgatggg gtttggtgat ataatgaaat
     1921 acctctttgt tactcataat tataccataa ttagtcacga atgcaaccaa tagaaaaaac
     1981 tacttagaac ggtagatcat cgctaataac aatgcattgt ccaccaaatg gattttgtga
     2041 agcattaaat acattgttat taattgtcgc tgcttttaac taggcctctc cccttctatt
     2101 ataaaagaag atatatatca tttcttaatt acctactacc ttaaaccaaa atttaagtac
     2161 tacctaattt aagtagtact taaacaatct aaaaaatagt tatcccaatt gttatgcttt
     2221 tataatacga ttatttgtaa caaaatgaat attatataat taaacactac catatttagg
     2281 tagtgtttaa ttattagcaa aacaaaaaat atttattcta ttctcttaac ttcaactaag
     2341 ttatgctgca cacctttata taaatcatat gcaactcatt agctagacaa actacaagca
     2401 tgtagtttta tctgttaatt atgtactacc taaataaagt agtttat
//
LOCUS       DACDYJ010000152         2405 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDYJ010000152 DACDYJ010000000
VERSION     DACDYJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.156"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..434)
                     /locus_tag="IWM51_002811"
     CDS             complement(<1..434)
                     /locus_tag="IWM51_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9972146.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAVRIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGV"
     gene            748..1170
                     /locus_tag="IWM51_002812"
     CDS             748..1170
                     /locus_tag="IWM51_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972147.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1180..1383
                     /locus_tag="IWM51_002813"
     CDS             1180..1383
                     /locus_tag="IWM51_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972148.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1594..2178
                     /locus_tag="IWM51_002814"
     CDS             1594..2178
                     /locus_tag="IWM51_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9972149.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          805 a          364 c          434 g          802 t
ORIGIN      
        1 taaactccat ttgctttcgc aattttaatg ccttgcgcct ggcgtcgttt agattcggta
       61 cgctcttgtt cagcgatcat ggccaaaatt tggatgatca gatccttaat gaatttatcc
      121 aggagagggt tgccaattgc ttcagccatg atcggtagac tggtgatgat tagctgtacg
      181 tttttcttct ttaggtaatt gactgaagcg atgatctcgt cgtaatttct tccaagtcga
      241 tcaatagctt ccacaataaa aatgtcttga tctcggacga aatccaatgc ttcttggaaa
      301 acaggtcgat gagtaatcgt tgcgcccgat tgtttctcga tgaaaattct cactgcaccg
      361 aattttttca tttcttcaat ctggcgttgt tcattttgat cgatggatga gacacgtatg
      421 tatgcaactc tcaaaatttt tacctacttt ctgcaactat ttaaaataca ccctaatcat
      481 acacgataaa accagatttt tcaatgttgt ggtcgtgtct agttagggta taccttatat
      541 agacaaaaac taatgatgga ggaaatagta tgtgtcactt ttctttttaa atggcaatat
      601 tttttgtaaa taaatgtcaa tcaagaaaat ttaaacgatt atgacattaa aaatatattt
      661 tatgaatttt ctagtatata acttgattta tctgttaaga tttattaaga aaacgttttc
      721 ttatttttga tttcgaggtg atagacaatg aaaaataagt ttattttatc tttaggtatt
      781 ttaactattg gaattagtgt aactggaact acaattccta catttgctga tagtacaaat
      841 gaaccaacta ctgtagtaga taataaccaa gaattaactc ctttagatag gaacggaata
      901 gccattggta acccaactaa cgacttaact gttactgaaa attatttaat agtttctcca
      961 ttacttgctc gagcagcttc taaaacgata cgagtgacag gaacgatgat ttttgataaa
     1021 gttggttggc cttctagacc tcctcaatca cggaattgga aagaaaaaag aaatggttat
     1081 tggtactatg gaaaattgta tttacaaagt tataaatcag ctggaaacaa ttggattgcg
     1141 acttattcag gaaccttaac tagaaagtag ggatttttta tgccagaatt tgtgaacaca
     1201 ccaaattttt atcaagatag cgatattcta agtaaaatta atttaccgaa agaagaatca
     1261 gctttacaag ttcagcgaac tttacaggag acacaaaaaa ttcaaaaatc aatttctaat
     1321 ctagaagatt ctgaagaaaa aagaaaaaaa agagaccaaa aaacatatca aaaagaaaaa
     1381 taatgatttt aaaaaaagag tgtctagtcg acattctttt tttctaattg aaagtgttca
     1441 taaaatcacc catttctgga cacttccttt ttttgcttta tttttcaaaa aaacaattca
     1501 tttcatgttc ataaacccat aacaatatct atgttcagtt aaactatatg gataaagtta
     1561 tgttacaata aaattaatga aggagtgatt tttttgaaaa taggatacgc ccgggtatct
     1621 actggtttac aaaatttgga tttacaagaa gacagcctaa ataaatttgg ttgtgaaaaa
     1681 atttttactg atcatatgag tggggcaaaa agtaatcgac caggattaga aatggccatt
     1741 gattttgttc gctctggcga tacacttgtc gtttggcgct tagatcgttt aggtagaaat
     1801 atggaagatt tgatttcaat cgtcaatcga ctgaatgagc gtggtgtgag ttttcatagt
     1861 attcaagaaa atattacgat ggacaaatca agttctacag gacagctcat gtttcatttg
     1921 tttgcggcat ttgcagaatt tgagcgtaat ttgatcctgg aacgatccgc agcaggacga
     1981 gcagctgctc gtgcaagagg acggtttggt ggtcgtcctg aaaaattgtc tgaacaagat
     2041 ttggaattac tgaagacttt agtggatagc ggtacaccta ttaagactat tgcggaacgg
     2101 tggaacgtct caagaaccac catttatcgt tatttggcaa aaatggagga gaaaaatgga
     2161 aagacaaatt ctcattagtt tttgtcattt aatgctgaaa atttttgcca tttatctaga
     2221 aaagtgacaa tagtatgaga attttttgta agtgtctact gataacttat tatcaaatgt
     2281 tgtaaggatt ttattcattt gttaatatta aagaacggaa ctccaaaaaa gtccgtactc
     2341 taatttgcag tctctcactc ttttagagtg agagttttta ttagattttc aaattaatta
     2401 gtaaa
//
LOCUS       DACDYJ010000153         2351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDYJ010000153 DACDYJ010000000
VERSION     DACDYJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.157"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(51..1598)
                     /locus_tag="IWM51_002815"
     CDS             complement(51..1598)
                     /locus_tag="IWM51_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP9972150.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IWM51_002816"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IWM51_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP9972151.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="IWM51_002817"
     CDS             complement(2043..2237)
                     /locus_tag="IWM51_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972152.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          660 a          488 c          421 g          782 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agtaaggtac tgttctatgg ggcgcttact ttataaaggt cgtccaaaac
     2341 tctatagtgc a
//
LOCUS       DACDYJ010000154         2255 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDYJ010000154 DACDYJ010000000
VERSION     DACDYJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.159"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(284..1624)
                     /gene="glnA"
                     /locus_tag="IWM51_002818"
     CDS             complement(284..1624)
                     /gene="glnA"
                     /locus_tag="IWM51_002818"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP9972153.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1652..2044)
                     /locus_tag="IWM51_002819"
     CDS             complement(1652..2044)
                     /locus_tag="IWM51_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9972154.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          642 a          499 c          381 g          733 t
ORIGIN      
        1 cgtctttttc aaaacctctt ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagt
       61 tcagccaatt caaaaacaag aaaagctgtt tttgcttggc tattcttatt acttgttgca
      121 gggcagcttt ttcacaacct ctccactaaa cggtgttcaa tcagctgacc ctcggtatta
      181 gctcaaaaaa cggcaaaata tgaggaacta tttttccgtt agtttctcta atactcaggt
      241 caaaacgctg atttcacagc ctctaattca cggtattcct gttttaataa agttctagat
      301 attgttctct ttcccactcg gaaacagttt gtctgaatgc cgcccattcg agacgttttg
      361 cttcaacaaa atttgagaag atatgttgtc ctaatgcgtc aatcattaca ttatccttac
      421 gcaattcttt gatagcatta tggatagttg aaggtaagtc ctggatatgt gcagcttctc
      481 tttcttcctc atccattaca taaatgttac ggtcaacagc tgctggcggt gtgatctgat
      541 tacggatacc gtctagtcct gcttgcaaca atactgccat tgccaagtat gggtttgcag
      601 atggatcaac tgaccgcaat tccaaacgtg tggataaacc gcgagattct ggtacacgta
      661 ctaatggcga acgattacgg ccactccatg cgacataaac aggcgcttca taacctggaa
      721 cgagacgttt ataagaattg actgttggat tgcaaacggc tgtgtaggca cgagcatgtt
      781 tcagcaaacc acctaagaaa tgataagctg tttcacttag ttccaattca ccgtctttat
      841 catagaaaac atttccttca tcattgaaca aagacatatt gcaatgcata ccagaaccat
      901 tgatcccaaa taacggtttc ggcatgaaag tcgcgtgaag tccatgcttg cgtgcaatcg
      961 ttttgacaac gagcttgaag gtttggatat tatcacaagc atctaccaca tctgcatatt
     1021 taaagtcaat ttcatgctgt cctggcgcta cttcatgatg tgaagcttcc acttcaaagc
     1081 ctagactttc taattccaac acgatgtcac ggcggcaatt ttcacctaaa tctgttggtg
     1141 caaaatcaaa ataaccacct ttatcattca aggtcgttgt gatcccaccg tcttcgtcta
     1201 atttaaataa gaagaattct ggttcaggtc ctaaattgaa agaggtaaaa ccaaggtcgc
     1261 gcatatcttt caatgctcgt ttcaaattcc cgcgaggatc gccggcaaat ggtgtcccat
     1321 ccggattata gatatcacaa attaaacgag caacttttcc atgagcactt tcccacggga
     1381 agatcatcca agtggagaga tctggataca aatacatatc gctttcttca attcgaacaa
     1441 atccttcaat cgaagaaccg tcaaacatca ttttattttc taatactttg tcgatctggc
     1501 tcactggaac ttctacattt ttgatcgtac ctaagatgtc tgtgaacatc aatcgtaaaa
     1561 atcgtacgtt ttcttcctca atgattcgtt tgatttcttc acctgttatc tgtttcttta
     1621 ccatggtatc cctcttcctt ttttaattta ttcagtcaaa gaattgtctt tgcaatcctt
     1681 gcgactgata atgacctgtt ttgcttaacc caccttgcgc caaaatctca tcacgaaaaa
     1741 tttgtcgaac gtcatgatct gtcaacggct tcttgttttt tgcttcttgt tttttcgcct
     1801 gtgccatttc gtatacacgt ttgatcccag ccatattcaa tccctctgaa agatagtctt
     1861 taatctcaag caacatatca atatcattca gcgaatacat acgtcggttt ccttcgcttc
     1921 gttccggatg aactaaatct tgttcttcat aatagcgaat ttgccgagca gacaaatcag
     1981 tcagcttcat aactgtacca attggaaata cggacataga tcggcgcaat tccttttctc
     2041 ccatcagacg tcccctttcg ttttataagt tggctaaagt gtaccaatta aaaaaaatat
     2101 tgtcaactgc atatgttagg ttttctaaca tgagatctct attttttata aaataacaaa
     2161 aaaataagaa cacaaaaaag gtatctagtt gattttcgta ccagatacct tttatttttt
     2221 attatactgt actatccttt attagtgttt ttatt
//
LOCUS       DACDYJ010000155         2244 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDYJ010000155 DACDYJ010000000
VERSION     DACDYJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.160"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            205..885
                     /locus_tag="IWM51_002820"
                     /pseudo
     CDS             205..885
                     /locus_tag="IWM51_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            1025..1564
                     /locus_tag="IWM51_002821"
     CDS             1025..1564
                     /locus_tag="IWM51_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972155.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVMKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          814 a          316 c          402 g          712 t
ORIGIN      
        1 cgtgaaatag aggggattta tttttctggt tacgtgatta aaaaatccaa aaaatgagca
       61 taggaacggt tatgatttta gggtgtgttt taatgagata ttgtgatttc attaacaagt
      121 gtatgataat cggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag
      181 aaaaaaaaca gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac
      241 gtcattattg tcgctgttgg ttactagctg agttacaatc taagctatcg tgaagttcag
      301 gaattgtgat atgatcgtgg aataaatgtt tgtcatacta cgatttatcg ttgggtacaa
      361 gagtacagca aagtcctcta ttatctttgg aagaagaaaa atagacaatc cttctattca
      421 tggaaaatgg acgaaaccta tatcaaaatt aagggacgtt ggcattatct ttatcgtgca
      481 attgatgcgg acggcttaac cttagatatc tggttacgaa agaaacggga tacgcaagca
      541 gcctatgctt tcttaaaacg actccataaa cagtttggtg agccgaaagc aattgtgacc
      601 gataaagcac cttctcttgg ctccgccttt agaaagttac agagtgtggg tttatatact
      661 aagacagagc accgaactgt gaagtatctt aacaatttaa tagaacaaga ccatcgacct
      721 attaaacgac gaaataaatt ttatcaaagt ctccgtacag cctcttccac gattaagggc
      781 atggaaacca ttcgaggaat atataaaaag aaccgaagaa atggaatgct cttcggcttt
      841 tcggtgtcta ctgaaatcaa ggtattaatg ggaataacag cctaagatat ttggagttca
      901 gagagggcgc gtttgatttt caaacttcgc aacagaaccg agaaccttgg gttttcaagt
      961 gctttttggt tcattaatct taaccatagt gtttttaaag tggaaaacaa gcaacaaaaa
     1021 aagtatgtgg agccttgtag gaatagttct tattattttt actgtgactg cagcaacaga
     1081 agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt ttagttatga ccaaaaaaga
     1141 tgttacacgt tatacatcct caagaactgg aggcctatat gactatgcac cattagatgc
     1201 attgaaatat aaagatcatt taaagaaaca tgaagtaaaa gttggtgatc ggtgggagaa
     1261 tgcatctcat aaagcatata attccaaaat agtctataca gtagaagatg ccgatgagca
     1321 aaatatcaca ttacctgtat tcaattattg gggaacagtt atgaaagtaa atgggaaaaa
     1381 gagcactata gaaaatgaca atggtttagt gtctattaaa ggtacgggga aaagaactat
     1441 agttgaggtt tcatctacgt atccaactac gatgattcta tctttattaa ttagttcaat
     1501 aacttttata ttcttattta cacgttattt aataagatgc tgcggtgaaa tgaaaataga
     1561 ttagatagat attataaata tgttgattct attttcatag caatggagaa aatacattag
     1621 caatatataa aagaaggtat caagagtcac agtgaattta ttccgaaaag tgaaaagagc
     1681 aaatataaaa tttaataaaa atatagaact aaataggaaa acgtagacgt aattttctac
     1741 gttttccttt tcatatatat taaatattac ggaaaattat acaaactaat ttagtgtttt
     1801 gtttgtaatt cctaattttg ctccactttt agataatttt taggtctatt taactcatca
     1861 gacattctat gctcattttt ctccaaattt gtttcaactc tttcgtactt aattttgcgt
     1921 atttgaaaat tcttctttta gagtactttt attacgatac tagtatgata tatatctata
     1981 gcaaacagtt aaaatcttcc taaaataagc gaatagcatt cctaaaacct ataaaatttt
     2041 tagatgaagc gacaagtgga caattaataa actacttgaa catgaaatta tggatcacct
     2101 ttcaaattta gaaaatggaa ttcttatact tattgcgcaa catttattga ttgcaaaatt
     2161 atgtgacaaa aagaactagt agaagaagga ataatggctt gataataaag tactacctat
     2221 taaaggtagt actttattta attt
//
LOCUS       DACDYJ010000156         2191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDYJ010000156 DACDYJ010000000
VERSION     DACDYJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.161"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            198..1622
                     /locus_tag="IWM51_002822"
     CDS             198..1622
                     /locus_tag="IWM51_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP9972156.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPKKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVTQTKTKTEQAVLTD
                     GFLRFSMHYGFETQVCNPRSGNEKGSMENKVGYVRYNFFSSTPIMKDFASFNEALAVE
                     LSHDRERIHYEKHVIINDLWNTEKTDLLALPDTDYPVFKEIEVKVNKYNEIKLDNERI
                     HVPRVRNYSIIYGLLRFDSYQLISTDGEIISEGLRPYMMKKRAIDWQAILPDWRRKSR
                     AMHYSRYWKYLPERIKLFLSHPDWKEQEKRLNQISGLLVTHDMLGIDTHFYDMFSAST
                     TTDAYDIDWHQYDAFLSLTGEVID"
     gene            1622..>2191
                     /locus_tag="IWM51_002823"
     CDS             1622..>2191
                     /locus_tag="IWM51_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9972157.1"
                     /translation="MDQAKSLETACKLLRLSHVLPTLVRKDLDYSFETKEDWLMYLLE
                     KELACREELKTARLLKQAKFPNKRTLEDYEWHDHICLPNATTKDELLSLSFIHQKENA
                     VFVGTPGTGKTHLACGLGQLAAKSGIDTRFWRVSDLVNELEHHWKNNTLEVFKHRFDK
                     VKLIILDEMGYVPFTKEGAELLFQLISDWY"
BASE COUNT          699 a          420 c          476 g          596 t
ORIGIN      
        1 agtcatctat ttcgtttatt cactttttca agtggacatt cattaagctg tttatggcag
       61 aataaaagta catacaagtg caacgaaaaa ctacacaact ccgcaaatcg attaagttaa
      121 gataaaaagc cattgcgggc aacctgaaat tcctctaaag ttatagtctc gacactgtaa
      181 ctggaggtaa gaaaggtatg ctcacaatga ccgatattaa tactatcaga aatttacgaa
      241 ataatcatga taaatccatt aatcatattg caaaggagct agatgtaaat tggcgcacgg
      301 ctaaaaaata tgcagatgat gatttgctgc cgcaacctaa agcacgcaaa aagactggta
      361 tgatgtatac agaaaagtgg ggaggcatcg tttccctatg gctttcagaa gatgctaagc
      421 tgccaaagaa aaagcgtagg actgctgaag caatgactac tgctttaaaa gccgatggct
      481 ttccaggatc ctaccgaaca gtctgtgtat tcattcaaga ttggaaagcg acacactact
      541 tggaaacact ggaagaccaa ggctttgagc gtcttgaaca cccgccagca gaagcgcaac
      601 ttgattttgg tacgatggaa gtttgtcacg atggagcgtt taaagatgtg aaggtattag
      661 tgatgacttt cccttttagt aatgcaggat tcgccgttgt actccccgcc gagaatcaag
      721 aatgtttgtt ggaaggcatg aaagaactct tcagccaatc aggtggcgtt cctcggaaaa
      781 tacggattga taatatgtca acggccgtta ctcagacaaa aacaaagact gaacaagcgg
      841 tgctgactga cggtttcctc cgattttcta tgcactatgg ttttgagaca caggtgtgca
      901 atccacgtag cggaaacgag aaaggtagta tggaaaacaa agtcggctat gtacggtaca
      961 acttcttttc atctacaccg attatgaagg atttcgccag tttcaacgag gcattagcgg
     1021 tggagctgtc acacgatcga gagcgaatcc attacgaaaa acatgtcatt atcaatgatc
     1081 tatggaacac agaaaaaaca gatttgctag ctttgccgga caccgattac ccagtattta
     1141 aagaaatcga agtcaaagtc aataagtaca acgaaattaa attagataac gaacggatcc
     1201 acgttccacg tgtacgcaat tattcaatta tttatgggct gcttcgtttt gattcctatc
     1261 agctaatcag cactgatggt gagattattt cagaaggact tcgtccttat atgatgaaaa
     1321 aacgagcaat cgattggcaa gcaattcttc ctgattggcg cagaaaatcg cgtgccatgc
     1381 attactctcg ctattggaag tatttgcccg aacgaatcaa gttatttctc agtcatccag
     1441 attggaaaga acaggagaag cgtttaaatc aaatctcagg tttattggtg acacatgata
     1501 tgttagggat tgatacacat ttttacgaca tgtttagtgc ctcgactact accgatgcgt
     1561 atgatattga ttggcaccaa tacgatgctt tcttgtctct aacaggggag gtcattgact
     1621 agtggatcaa gcaaaaagtt tagaaactgc ctgtaagttg ctgagacttt cccatgttct
     1681 tcccactttg gttcgcaaag acttagatta cagctttgaa accaaagaag attggttgat
     1741 gtatcttctg gaaaaggagt tggcttgccg tgaagaattg aaaactgccc gactcctaaa
     1801 acaagcaaag ttcccaaata aacgtacctt agaggattac gaatggcatg atcatatatg
     1861 tttgccaaat gcgacgacta aagatgaatt attgagtcta tcgtttattc atcagaaaga
     1921 aaatgctgta tttgtgggta cacccggtac cggtaaaaca catctagctt gtggattggg
     1981 tcagctggca gccaaatcag gtatcgatac acgcttttgg cgggtatcag atttagtcaa
     2041 cgaattggaa caccattgga aaaacaatac cttagaggtc ttcaagcatc gcttcgataa
     2101 agtaaaatta atcattcttg atgagatggg ttatgtgcct ttcactaaag aaggcgcgga
     2161 actgcttttc caattgatca gcgattggta t
//
LOCUS       DACDYJ010000157         2155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDYJ010000157 DACDYJ010000000
VERSION     DACDYJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.162"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..850
                     /locus_tag="IWM51_002824"
                     /pseudo
     CDS             1..850
                     /locus_tag="IWM51_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            954..2126
                     /locus_tag="IWM51_002825"
     CDS             954..2126
                     /locus_tag="IWM51_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAP9972158.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSTIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          837 a          331 c          364 g          623 t
ORIGIN      
        1 tagctttgag ccaactgatt catttcagta tcttagcaaa gatattgaaa ttgaagatat
       61 ttataaaatt ttagctcaag agctatctaa aaatgttatt agtttgccaa aggtttttta
      121 ttttccagcc gaaagaactg gtataaatgt ctttaagaac gaactaaatg aaagtcgctt
      181 aaaaacatat gataccttgc tgaatactgt gcaatttact aatttaacta accaaaaaaa
      241 gaaagctgcc ctcaagtacg atctgttaaa aaagaatatt gagctgttaa tggagacaac
      301 ggataatagt tcttatccaa agccaatatc ggattatatt aattatctga ataacatgaa
      361 aaatagatat gaacagaaaa atgtcaataa tatagcatca tatattagag atgagattat
      421 tatgggtaaa tatgaaattg acgaaaaaga taattctgta agttttagac aaaagtttgg
      481 taaaaataga tatagaaaag aacgaattcc ttttcacgtt gtgtcttctt cgattaaatc
      541 tctatatgga ttagattatt acttagaaaa ttcaagtaaa gaaggagact atcttattat
      601 tgatgagcca gaattgagtt tacatccaga aaatcaggtt aagttagcaa aagtaattac
      661 agatatagtt gagagtaaaa ttaagctaat tacttctacc catagtgatt tatttgtacg
      721 tgagcttaca aatattgtta ttgaaaataa attaaatagt aagaataata aaaaaaataa
      781 attgtctgat aaaaatatag gcatatataa ttttgtagga aataatgata taaatgaatt
      841 agaaaaaatt tcgataaagt ccgtataatt gtgtaaaagt aaaaaggcca tataacagtc
      901 cttttacggt acaatgtttt taacgacaaa aacataccca ggaggacttt tacatgaccc
      961 aagtacattt tacactgaaa agcgaagaga ttcaaagcat tattgaatat tctgtaaagg
     1021 atgacgtttc taaaaatatt ttaacaacgg tatttaatca actaatggaa aatcaacgaa
     1081 cagaatatat tcaagcaaaa gaatatgaac gaacagaaaa ccgacaaagt caacgaaatg
     1141 gctattatga gcgcagcttt acgacacgtg taggcacgct agaattaaaa gtacccagaa
     1201 cacgtgatgg ccatttttca cccacagtgt ttgaacgtta tcaacgaaac gaaaaagccc
     1261 tcatggcttc aatgttggaa atgtatgtat caggcgtttc aactcgtaaa gtatcaacaa
     1321 ttgtggaaga actttgtggt aaatccgtct ctaagtcctt cgtttctagc ttaacagaac
     1381 agctagaacc tatggttaac gagtggcaga atcgtttatt atcagaaaaa aattatcctt
     1441 acttaatgac cgatgtactc tatataaaag tacgagaaga aaatcgagta ctctcaaaaa
     1501 gctgtcatat agcgattgga ataaccaaag atggcgaccg tgaaattatc ggcttcatga
     1561 ttcaaagtgg cgaaagcgaa gagacctgga caacattttt tgaataccta aaagaacgcg
     1621 gtttacaagg tacggaactc gttatttctg atgcgcacaa aggattagtc tctgccatta
     1681 gaaaatcctt caccaacgta agttggcaaa gatgccaagt tcacttccta agaaatatct
     1741 ttaccaccat tcctaaaaaa aattcaaaat ctttcagaga agctgttaaa ggaattttta
     1801 agttcacaga tattaactta gcgcgtgagg ctaaaaatcg attgattcat gattatatcg
     1861 atcaaccaaa atattcaaaa gcttgcgcat cattggatga tggattcgaa gacgcctttc
     1921 aatataccgt acaaggaaat tcccacaatc gactaaagag taccaatcta attgaacgac
     1981 tgaatcaaga agtacgcaga agagaaaaga ttattcgcat cttccccaat caaacatcag
     2041 ccaatcgctt aattggagcc gttcttatgg acctacatga tgaatggatt tattcttcaa
     2101 gaaaatacat caattttgat aagtagaaat ggtaaaaaca ttgtatagca tttta
//
LOCUS       DACDYJ010000158         2116 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDYJ010000158 DACDYJ010000000
VERSION     DACDYJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.163"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            115..492
                     /locus_tag="IWM51_002826"
                     /pseudo
     CDS             115..492
                     /locus_tag="IWM51_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            540..1940
                     /gene="yfcC"
                     /locus_tag="IWM51_002827"
     CDS             540..1940
                     /gene="yfcC"
                     /locus_tag="IWM51_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9972159.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMVLFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1973..>2116
                     /locus_tag="IWM51_002828"
     CDS             1973..>2116
                     /locus_tag="IWM51_002828"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9972160.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          585 a          360 c          451 g          720 t
ORIGIN      
        1 gtcattgata tcagtcctat tcgagtatgt tttctgcaaa aaacattgta ctcctaactg
       61 agatcaatgg cttttttgat gcttcacttt tacacaatta tatggacgta atcaaaaaat
      121 gtttccacag aggacttctt taattattgt cgattagagg aaagtggtgt aacagtcact
      181 agcggtacag atttaccgct gtttattact gatattcctg aatcgattat aagagcagta
      241 ttccgtcgtt ttcctgaagg agaagaagtc tgggagaaag aaacaagctt cacggcaacc
      301 gagctgctga aaagttttac aagcaacagc ttcaaagtaa atggtttatc ggagtacgga
      361 gaaataaaag tagggaaaag cgccagctta gctgttttca gcgaggacct ctgtactgat
      421 gactggcaaa aagtgatggc tgcggaagtt atcgctacct atcttgatgg tgacttagtt
      481 tacaagaaat aatgcttttc gatataacgt tataaaacga attaaagaga ggttaaaaaa
      541 tggaaacaat gaaaagaaaa ttcaccttcc cacatagtta tacattgatt tttatcttaa
      601 ttattttggc tgcaatttta acttggataa ttcctagtgg tcagtttgag acagttgttg
      661 aaaacgtcgc aggtatggaa aaaacaacgg ttgttccggg aacctatcac caaatcgata
      721 aggcagatta tggacaaggt ttttcctcta tactaatggc accagctgaa gggattattt
      781 cagctgttga ggttgttgct tttgttctca tcgttggtgg tgccttcggg attatcctaa
      841 aaacgggagc catcgatcga gggctgttta ccttagcaaa agttctggga gacaagggga
      901 tcttagttat tccgatgtcg atggttctat tcagcttagg aggatcaacc tttggcatga
      961 gcgaggaagt cattccctta tttgcggtgt ttatttctct gatgttttct cttggctatg
     1021 attccatgac tgcgatcttg attctatttt taagtacaca agcgggatat gttgggtcga
     1081 cgattaatcc cttcaacgtt ttaatcgcac agggagtagc tggcgtttac ggaaatcccc
     1141 tgctttggtt tcggatgcta tcctggattg tcattactgg gatttcaatt gttttcacca
     1201 tgctttatgc tagcagagtc aaaaagaatc cactgtcctc tattgtttat gagagtgatt
     1261 taaaggttaa gcataagttt caaaattttg acactgaaaa agagttttca acacaagata
     1321 aactaattat cgccggtttt atcgtaggtt taggaatcat gatttggggt atcatttcca
     1381 agggatggta tatggttgaa atttctgcac tattcttagg attaggtctt tttgctgggg
     1441 gcatcgctag attttctcaa aaggaaattg ctgaagcttt tgtcgaaggc tgttcagatt
     1501 tcgcctatgc tgctgtaatc attgggctag ctagaggagt cttggtaatc ttggaaggtg
     1561 gaatgattat tgacactatt ctaaacagct tggctaacct tttaggtggt cttccaaaat
     1621 tcctgttcac tacgattcta ctcatttctc aagtaattgt ttcctttttt gttccatcct
     1681 cctcaggagc tgcggcatta acgatgccgg ttatggcacc attgggtgat ctaatgaatg
     1741 taagtcgtga tgttgttgtt ctaagtaacc agtttggaaa tggtttaatg aatattatct
     1801 ccccaactag tggtgtatta ctggcaggat tagccatcgc agagattaat ttctctaaat
     1861 ggtttaaggt tggcttcaaa atatttgcca tcttaaccct tgtttctgct gttttgttat
     1921 acattgctac actcttttag tcttaatcat ccattaatag gaggcaatag ttatgattaa
     1981 aataaatcaa ttagcaggaa tgaatatcca ctatttattt tattctctca aagaattttt
     2041 cgcttatcaa cagaaaattg gtatttcttc tgttgaactt tggggaggtg ctccgcattt
     2101 tttcatagat gctttt
//
LOCUS       DACDYJ010000159         2110 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDYJ010000159 DACDYJ010000000
VERSION     DACDYJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.164"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(490..1104)
                     /locus_tag="IWM51_002829"
     CDS             complement(490..1104)
                     /locus_tag="IWM51_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9972161.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1710..2087)
                     /locus_tag="IWM51_002830"
     CDS             complement(1710..2087)
                     /locus_tag="IWM51_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972162.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
BASE COUNT          706 a          409 c          319 g          676 t
ORIGIN      
        1 tgcaaaaaaa ataataaaca cttataaata tccattatct atccttcaat ttttgtgtat
       61 tttatttcta ttagaaaaag gtatcctgtt ggacaatttt gttatatcag gtaaacttca
      121 ttgcaataat gataacaaat gtactataat tactttgtaa agagttttgg ggaagactct
      181 ttcatcaacg tgtattaaga gacaagaaag aatccatatg gattctttct tgtctctttt
      241 tccactactt tttatttttc tgacactaca tattcttata actcatttct cataactgtg
      301 aaacaactta taaaaataac aaactttaaa caaaacaagc ttaaaatcaa agatgatcca
      361 tcttaaaata actaaaacaa aaaaagtagt cgttgttaat cctcattaat aatcgttttc
      421 tttttttcaa acgctccatt tttatgtgga acattatttt tttaaggaac tttgtcaaca
      481 tgtggataac tattttccac ttgtgtcctg gactagctgt tttgctgtaa aatacaaaac
      541 ttgctgcagc aaaaagatcg ttgcaagaga ggctgttccg cagatcgctg gatctagatt
      601 tggcaaagtc aagactgcat catgtaaatt ggcgtggttg tccaaaacca aatcagcaaa
      661 ttcaaataat cgcaaaccac tgctatgttg cgatttgata cttcgtgaca aatcaaatcc
      721 agtaacaatg attaatggat gaccattttc tttgatccac tgagctagtt cgatgattgc
      781 cgggtttctt ccttcattcg aaagaagaag aaaaatgtct tctgatttga cagaaagctg
      841 atcaataatt gcctgtcctg caccttctag cgtttcatag acacctccgg ctggattttc
      901 aatcaatata cttggtacaa gatccggggt ttgatcccaa aattcaaatg ctacaccact
      961 tagtgtgcgg ctagcatata tctgcaatct accaccttga ttaatttgtt tcgcgatcca
     1021 agtgccagct tgaagcatca gcggctcttc tatccttaat ttctcttcca attggtgata
     1081 atagtcaaaa taacataaac tcatttatct ctcccgccta gctatacttg acatgaatag
     1141 aaacagctgc ctaatctaag gaagtacata aaaaaacagt agttagacag taacacatgt
     1201 ggtctttgct gtttcaaaca caaaaaaggc attgaacgaa tacgcccaat accttgacat
     1261 agcaaataaa acaacgtgca gaccatggaa aatatctaca taggggtaga attttcaccc
     1321 tagattaggt acaataaatt aggtcgatgc actgcattga ctatggcaaa atgatcggtc
     1381 agggtgtatg tgtgtgactg gtcattccct gattcttagc gccaactaag ggtcagggag
     1441 ccttttttta ttcagctgtt tctatctgaa tttattgtaa gtaatctctt atccaagtgc
     1501 aagcaagaaa atacaaaaaa acattggtat gatttatata tatataaata acataaaaaa
     1561 aggaatggct tatcccgata caacaagcat tcctttaaaa aagattcttt tatataagtg
     1621 aaaaaagctt ttttccacca attaaataaa tctctcatct caatagttta tccattattt
     1681 tatctttttt tgcgtatacc tactaaatat taccgtcttc ttctcgttct tctactttgt
     1741 tcgagagaat ctgtactaga aaaagtagtt gtatgtctac cttttctgcc tatccgagac
     1801 tttcttttca tatatgggat ataaatgatt agatacagta cagcaaaaaa taaagcaaat
     1861 acgcaagctt cccaactacc taacgccaat tcaaccatca aggtagcaac cccaacggat
     1921 gctgcatacc agataaaaat cagaaacgtt tctttttttc tgaccatgcg aaacatgata
     1981 aatagactaa taagcaatag tggcaacaca aatatccaat tgcttccttc catcagccac
     2041 ttgagtatat caagcagcga ctgtgcaaca gcgactaatt ttcccatagt gaacacacac
     2101 ctttttttat
//
LOCUS       DACDYJ010000160         2060 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDYJ010000160 DACDYJ010000000
VERSION     DACDYJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.165"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(71..226)
                     /locus_tag="IWM51_002831"
     CDS             complement(71..226)
                     /locus_tag="IWM51_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010001600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9972163.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            843..1064
                     /locus_tag="IWM51_002832"
     CDS             843..1064
                     /locus_tag="IWM51_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972164.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1065..1322
                     /locus_tag="IWM51_002833"
     CDS             1065..1322
                     /locus_tag="IWM51_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972165.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKAAKSAVPTGFLTKQPYIF"
     gene            complement(1458..2060)
                     /locus_tag="IWM51_002834"
     CDS             complement(1458..2060)
                     /locus_tag="IWM51_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9972166.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKIRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          679 a          465 c          313 g          603 t
ORIGIN      
        1 tcatgctata atcaccttaa ttgaacaaca gtctaacagc aaaagcagtg cgccaacact
       61 aatgcttttg ctattttttt tgctcatttt cctttccgtt agcattaacc caaattgtaa
      121 taagaccaga cttactaaga cccatttgtt tcgcaatttt atcaagatta tctgccactt
      181 ccttagtaag cgttacaacg actctctttt tttcattact gatcataatt cgtatcctcc
      241 aacgactttt tcttaaaaac catagtaaca aaaaagtaac taaggtgcaa cagtttagat
      301 actaattagc gaataatagg ccttaaaccc ttggcattac tgattttgtc aaaaaaaaac
      361 gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa tgaccaataa
      421 ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa ccaaaaaaca
      481 aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg caggcgccac
      541 gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt cgctaccagt
      601 cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc cgataactaa
      661 acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag cattgatttt
      721 ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt atattataag
      781 cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg gaaggagcat
      841 aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg accagcgaac
      901 ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa gaacatctga
      961 ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc ccagaagccc
     1021 ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct aaagaaacaa
     1081 ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat tttatcgaat
     1141 tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc acaaatattc
     1201 tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca ccttcctaca
     1261 ggaaggcggc taagtcggct gtgccaactg gttttcttac aaaacaacct tatatttttt
     1321 gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta cctacttttg
     1381 gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca caaaaaaaga
     1441 gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc accatgacga
     1501 ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag cggaaaccaa
     1561 tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt gatattctta
     1621 gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact cgttactgac
     1681 aaataactag caatttctgc accaccatga gcgataatat cacttttagc atattgaaat
     1741 ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt agcacatttt
     1801 tgcgggtttg gagaccgaat cttttgagtt atctcacgta tttgatcata cgtcttttta
     1861 ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc aggattcaca
     1921 tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa aaactccctc
     1981 caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt gcgttcatct
     2041 ttagtttttc tactcgtcat
//
LOCUS       DACDYJ010000161         1992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDYJ010000161 DACDYJ010000000
VERSION     DACDYJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.166"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="IWM51_002835"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="IWM51_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9972167.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="IWM51_002836"
     CDS             852..1040
                     /locus_tag="IWM51_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9972168.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="IWM51_002837"
     CDS             1053..1610
                     /locus_tag="IWM51_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9972169.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="IWM51_002838"
     CDS             1642..1884
                     /locus_tag="IWM51_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9972170.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          554 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc ct
//
LOCUS       DACDYJ010000162         1981 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDYJ010000162 DACDYJ010000000
VERSION     DACDYJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.167"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..367
                     /locus_tag="IWM51_002839"
     CDS             <1..367
                     /locus_tag="IWM51_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9972171.1"
                     /translation="KKQIVPKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYL
                     PGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESA
                     NDLKIELDTWLLGLDVTEK"
     gene            567..770
                     /locus_tag="IWM51_002840"
     CDS             567..770
                     /locus_tag="IWM51_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972172.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            1332..>1981
                     /locus_tag="IWM51_002841"
     CDS             1332..>1981
                     /locus_tag="IWM51_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9972173.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVDDK
                     VHPLKLSWSIDRITIVGKLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQ
                     LFDQYGEDIAYIEMLKFHPDKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIA
                     CDILGVPDDFITQYRIVDPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQER"
BASE COUNT          658 a          305 c          461 g          557 t
ORIGIN      
        1 aaaaaaacag attgtaccaa aagaaatcga aacatggtgg cgtatagaaa tgcagctaag
       61 acgtggcaaa gcaacagatt ggcatgcgat ggttcgggaa agtttagata gctttgcaag
      121 tcctcattac ttgcctggag atgtaaaacc tgttgataga attatgataa aagggctgaa
      181 tcaagatcat tcagagtggg cgtatattag cagaaatttg aaatatagat tgcgcaaatt
      241 gttgaaagaa gaaagccaaa acgatgagtt gacgaatcat ttacgagaga cgtttaagga
      301 atctgcaaac gacttgaaaa tagaattaga cacgtggctt ttgggtttgg acgtgacaga
      361 gaagtgatac cgaataagaa aagtcagatg agtgagagta aatttacgtc aatatttaat
      421 gacgtgaatt tacgataagc gattattttg cgtgaagcaa aataaagcgt aaaaggagtt
      481 gatagcgtga tacgctttcg gctccttttt tcgttgtcac ttgtgacagc gaaaaccctc
      541 ggagagcgtt tggaattgtg acagccatgt cacaattagg aaaatgttac tctttgtcaa
      601 agagtaacag aaaggtttac actcttttta ttagcgacag cgcgaaggag gggttcacct
      661 tagggtttag tatttcgttc aaaaagggaa caaagaatac aagcatttct cataataatc
      721 gtgagttaac tgatagccaa aaaatcaatg gtttgtctgg tagaaaataa ttagcagatt
      781 tataagtggg aaataaaaag ctgaaagaag aaaattgcat ttcaaactac attttctaca
      841 atagctctaa aacggcatat tttgcgttct gagcggtttt agcatttatt tcgtttagtt
      901 atcggcacaa tcgttaaaac acgttagagg agcgaaaatg gagcctgtaa ggcgactggt
      961 agcggaacga tcgtgttaga ttgtgaaagc cgatagctga atgaaataaa ctgcgggtgc
     1021 gccctttcca tttcggcagg aggaggctca aggggttcgg ggaatgaaat tccctgaggt
     1081 ctttttattt ttttaggagg agtgctataa tcaaagaaaa cgagaaaaaa gagcaaaata
     1141 aaagccgaac ggtgttggaa gcacccttcg gctaattacg gaaactactt cacctagctc
     1201 cgtaactcag gttcattata cgaatatttt taggtttctt caagctagag atttttggat
     1261 atgaattttt agggttacgt gaactctgga aattctttcc aaaagtctct tttttcgtct
     1321 ggaggacgaa aatggcaaat gaaaatcagt tgagtgaaat tcaatcaaaa attgaaggtc
     1381 gaggggcaga aaaaagaaca acgataaatc agctcgaaaa acttaaccgt atttctaaca
     1441 ggcggttaag tgttgacgat aaggtacacc ccctaaaatt aagttggtca atcgatcgaa
     1501 ttacaatcgt cggaaaactg aatgatacca tagcttattg gacgcaaaat ggagttgtgt
     1561 ttgtcgattt tgagcaatta atgcgtttga atgaaaatca agggtatttg aagtcggttg
     1621 gtacaaatgg ttggcaatta tttgaccaat atggagaaga tatcgcctat atcgaaatgt
     1681 taaaatttca ccctgacaaa ggtcgtattg attttaatcc aaataaaatt ggcgagtttt
     1741 taaaggggtc gatgaaacaa ttcattcatg atttgtttct ggatgcgcat ttctcacgtg
     1801 cagatattgc ttgcgatatt ctcggtgttc ctgatgattt tattacccag tatcgaatcg
     1861 ttgaccctgt gtcttttaag ccaatttatg gccgttctgg caaattagaa acagcgtact
     1921 ggggtagtcg tgccagcgag cgacaagtgc ggatgtacaa caaaaagctt gaacaggaac
     1981 g
//
LOCUS       DACDYJ010000163         1974 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDYJ010000163 DACDYJ010000000
VERSION     DACDYJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.168"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            37..1068
                     /locus_tag="IWM51_002842"
     CDS             37..1068
                     /locus_tag="IWM51_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9972174.1"
                     /translation="MIAELKPKHERQNFLVGDDRLDHAVAFLWKDPQTGETVGASYQG
                     TIVDFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKFFESSIDLLSYAALNREKLQ
                     DAWLVSMDGLKHHVISHYVEESISELRRKQTFPQSIEICVDNDRAGHIFYEKEQMKGI
                     VDPFTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVEPEAIMAIHKTETNLQLTNQ
                     LVSAHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKVCERADGTYNFDRFYSRKAN
                     IKDVNAGILLSYKAEQYYKGYKKHEHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLF
                     QQGRQQERE"
     gene            1079..>1974
                     /locus_tag="IWM51_002843"
     CDS             1079..>1974
                     /locus_tag="IWM51_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9972175.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMN"
BASE COUNT          710 a          346 c          428 g          490 t
ORIGIN      
        1 ccagagtggc tgattgatta tgcagaaaaa gaagggttga ttgctgaact aaagccgaaa
       61 catgaacggc aaaatttttt agttggagac gaccgtttag atcatgcggt tgctttttta
      121 tggaaagatc cacagacagg agagacagta ggggcaagtt atcaaggaac gatcgtcgat
      181 tttaatcgtt tcggtaaacg gggaacgtat aaacacattg acaagaatcc tacgccaaat
      241 cacggattta atttaaaaat aggcgatccg aagcatttaa aattctttga aagtagtatt
      301 gatctgttaa gttacgctgc attgaatcgg gagaaactac aagatgcctg gttagtttcg
      361 atggacgggt taaaacatca tgtaatcagt cattacgttg aagagtcgat ttctgaatta
      421 agacgaaaac aaacatttcc tcaatcgatt gaaatatgtg tggacaatga tcgtgcagga
      481 catatttttt atgaaaaaga acagatgaag ggaatcgttg atccttttac caataagaaa
      541 atccgttgtg agcgaggaat tccaaatgat tggcaagtac ctaaagaata caaagctact
      601 tatgaagcag tcgcaaaaga aatgagtgtt gaacctgaag caattatggc catacacaaa
      661 actgaaacta atttacagct gacaaatcaa ttggtgtctg ctcatgatgt tcaatctacg
      721 tttggaaaga tgttggcgaa gggcgaacca gtagaaacaa ttgatttgaa agaagcatgt
      781 accacggtcg cgaaggaact gaaagtatgt gaacgcgcag atggaacata caactttgat
      841 cgtttttata gtagaaaagc caacataaaa gacgttaatg caggaatcct tctttcctat
      901 aaagcagaac aatactacaa aggctataaa aagcatgaac atgaatttgt acctgaagtg
      961 aaaaaggatt ggaatgatca attgaagcat gagatccagc aacaagaaat cagaaagcaa
     1021 aaacgtgcga tgttgtttca acaaggcaga caacaagaaa gggagtgaag tcgtcactat
     1081 gaaaaccgtc attttagcag aaaaaccgtc acaagcgaag gcttatgccg atagcttttc
     1141 taaagctacc cgaaaagatg ggtattttga gatccaagat cgattgttta caggagaaac
     1201 tgtgatcacg tatggttttg gtcatttagt cgagttagat tcgcccgata tgtacgatga
     1261 aaaatggaaa caatgggcat tagaacattt gcctattttc cccaatcaat accactatca
     1321 tgttcctaaa gataagaaaa agcagtttag tgtcgtcaaa cgacagctgc agtcagctga
     1381 tacgattgtg atcgcgactg atagcgatcg tgaaggggag ctgattgcct ggtcaatcat
     1441 tcaacaagca ggcgccaatc aaggaaaaac ctttaaaaga ctttggatca actccttaga
     1501 aaaagaagcc atttatcagg gattccaaca gttacgagac gcagaagaga cctatccaaa
     1561 atttgaagaa gtacaagcac gtcagatcgc cgattggtta attgggatga acggtagccc
     1621 actgtatagt ttactcttgc agcaaaaagg cataccagga agtttttctt taggtagagt
     1681 ccaaacccca acactctata tgatttatca attgcaggaa aagattagaa acttccaaaa
     1741 agaaccatat tttgaaggaa aagcccaagt aatcgctcaa aatggcgcat ttgatgcgaa
     1801 gcttgatcca aatgaaaccc aagcgacaca agaagcattt gaagcctatt taaaagaaaa
     1861 aggtgttcaa ttaggaaaac agccaggtac gatccttcaa gtcgaaacag agaaaaaaag
     1921 tgcggctagc ccacgtcttt tttctttatc gagccttcag tctaaaatga atca
//
LOCUS       DACDYJ010000164         1953 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDYJ010000164 DACDYJ010000000
VERSION     DACDYJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.169"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            156..1862
                     /locus_tag="IWM51_002844"
     CDS             156..1862
                     /locus_tag="IWM51_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP9972176.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT          698 a          387 c          353 g          515 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caa
//
LOCUS       DACDYJ010000165         1888 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDYJ010000165 DACDYJ010000000
VERSION     DACDYJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.170"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            715..1800
                     /gene="xerS"
                     /locus_tag="IWM51_002845"
     CDS             715..1800
                     /gene="xerS"
                     /locus_tag="IWM51_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP9972177.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          703 a          317 c          257 g          611 t
ORIGIN      
        1 aaaaccacta gcccgccagc tagtggtttt tggtgtatca tgaattacct tttcgttaaa
       61 acaattatac catatttcct aaaaaataca attaatagac gaattatcat taccactttg
      121 atgaaaaaag ctgacataaa tttttctttt atttttataa tgaataaaca tttatcaata
      181 agcttttgaa aggcaggatc gtactttgtt gatgattgag atattgctac atcaatctca
      241 gcagttaaga tttttttgaa ttgttgcttg aattttttct ttatctaaaa aaattgtatc
      301 tccaggatgc aacccatttt ctttaagaaa ttccttttaa attgttaata ctactgagtg
      361 ccccacttct ctgaattttc tttaacttct ccctcccctt ttttatttgt atatacattc
      421 atatacgctt gttaatatca aatcaaccgt agtctctctt atgtctaatt atatagaaag
      481 attgttcttt ctatataatt tagacaattt aaaaaattaa gattgcctcc ttctgtttcc
      541 tttcctattc atgtatacca tttattttaa attattaaat ctatacctat ttatctgctt
      601 ttttgaaata aattaataaa tttcagagta taccaattga caaaacactc tataattggt
      661 atagtccaat tatagagata ctcctctccc ctacttatcg aaaggatgaa taacatgaaa
      721 aatacagaaa tatatcaaaa aatagataca atcatacttc atatgcccga ttatataaaa
      781 gattttgttc aagatcgtga ggataaagat caatcacctc gaactacttt agaatatcta
      841 aaaaattata agctctttta tgaatggctt ctgagtgagt ctatcgttcc cgatacttca
      901 atcacctcga taagacagtt aactgcctat gatctaaact tgtacaaatc tcatttgaaa
      961 cgtcgcacaa aggaaaatgt aaaaaaagag acaacaaaaa ctaagctcaa agataataat
     1021 aacttaggtc tatctacttc tactatcaat agaaatatca cagcattaaa ggttctattt
     1081 aaatatcttt cgaaaagttc caataatcct ttaggaaaac catacttaga aaataatcct
     1141 atggatcaag tagcaactat cacagataaa actacgttag ctgcccgtgc aaacgcaatc
     1201 gagaaaaaat tatttctaga cgaagataca caaaattatc tagattatat tgccaatgaa
     1261 tataaaaaaa ctttgagcaa acgagctttg atctattatc aacgagatgt agaaagagac
     1321 ctggcgatca atgcattgat tttaggatct ggattaagac tctccgaggt tgtaaatatt
     1381 aatttagagg acctatcttt agaaaagaat aatgtggtag taactagaaa aggaaataag
     1441 agagacgcag taaatatagc agcatttgca atggaatatt tagctaatta tctagctatt
     1501 cggaaagaac gatacaaagt aacagaaaac gaaaaagctt tatttctagc aatttatcaa
     1561 ggagaagcca agcgtatatc cggtattgca atcgaacgga tggtagcaaa atattcaaaa
     1621 ggattcagag tccaagtcag tccacataaa ttacgtcata ctttggctac tagactctat
     1681 caacagacaa attcattggt attaactgcc caacaattgg gacatacaag tacaaataca
     1741 acaactttat atactcatat agacaatgca gctacaattg atgctttgaa cgctttgtaa
     1801 atgtagtaaa aaatagtata ataatgaagc ctgtctctaa aattagataa aagcccaact
     1861 ttaaaggcaa gcttcattat cattttta
//
LOCUS       DACDYJ010000166         1875 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDYJ010000166 DACDYJ010000000
VERSION     DACDYJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.171"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            207..>1875
                     /locus_tag="IWM51_002846"
     CDS             207..>1875
                     /locus_tag="IWM51_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9972178.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLH"
BASE COUNT          665 a          281 c          361 g          568 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatg
//
LOCUS       DACDYJ010000167         1724 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDYJ010000167 DACDYJ010000000
VERSION     DACDYJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.174"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            258..1184
                     /locus_tag="IWM51_002847"
     CDS             258..1184
                     /locus_tag="IWM51_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972179.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYMFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILITRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGNG"
     gene            1290..1553
                     /locus_tag="IWM51_002848"
     CDS             1290..1553
                     /locus_tag="IWM51_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9972180.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            1553..>1724
                     /locus_tag="IWM51_002849"
     CDS             1553..>1724
                     /locus_tag="IWM51_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9972181.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRY"
BASE COUNT          647 a          205 c          314 g          558 t
ORIGIN      
        1 tatttttaat caaaattgac gtaactttta ataaatacta cctaaataag gtagtattta
       61 tttttgtgtt ttaaaagagg gagtccaata tatctatggg aaacttctta aatattatgt
      121 tggtacatga aaatatacat ccctgctaga ctatttacaa cattttactt tgtatgtatt
      181 cttgttttaa aaggaatggg ataaaatata ggtacaataa ctatttgatg ctaaatataa
      241 ttaatggagg agagatagtg aacatactta atgaaaaaag atatgtatgg tttgctggta
      301 aaaaatatat tactactttc aaaagattat ccgagttaat gcagcagact gttaaaagtg
      361 ctatgcctga aggatacaag tttaattttg ttcaatcttc agtatctact gaaagtatct
      421 atttttcaat tctttctgag agtaggcttt ttcattttag aataagttct catgtaaaga
      481 aaactgatgt tgatatatat atgttccgta caaataatta taaaaattta aatgatttga
      541 gtagaagtat cagtgaatat ctcaagggga atagctatct caagattcga ttttcacata
      601 tattaatttt actaccatat ttgaatgaga atttcaccct aattcaagta gataagtctt
      661 tcattgcctc aggtaatcaa ggaaatatga atattacaag taacaaaacg tcacgtgtgc
      721 tattggcgtt gatgggttac ggaattcttt atagagatga ccaaaatggc cagattctaa
      781 taactcgggc cgggcgttca ttattaatga aatatagaaa atttataccg gaagattttc
      841 aaaacttaca gttagaaaac atgcgtttag aagatttatt tagattagca gaagatgata
      901 agtatagatt tgaagttttg aaaattcaaa agaatgaact attgaataag ttatatcctg
      961 agaatgcgat aaaagcacat tttggtgatg ctaatcatat gtatagttgg ttcattccta
     1021 aaagatttag agggaaaatc aatgctggag atcaagtgaa agtactcaat cgaaatagta
     1081 cagatgtcgt aatgataaca gatatctatg tagaagatga agatataatc aaatatatga
     1141 agcctgtgat aaaacttgtt aataaaaata aaggaaacgg ataacacttt acaatgctgt
     1201 tttggtactc caatttaaac aaaacaatcg aatttacgta cttaattaag tacgttagaa
     1261 tggagatata aattagaggg ggagtagata tggcaaaaac tgtcttaaat cctagctcag
     1321 cacggaaaaa tttttataag ttgttgaaag atgtaaatga gaatcatacg gaaattgaaa
     1381 tcattagtga gcgcagtgaa aataatgcgg tgcttattgg tttggatgat tggaaagcaa
     1441 tcaaagaaac gctattactt gagcaaatag gcactttggg tgttgttcgt aagcgagaaa
     1501 aagatgatag tggatttact aatgttgatg aaattgattg ggataactta taatgaataa
     1561 ctattctgtg atgattaaaa actcggctaa agctgattta aagaaaatca aacagtctaa
     1621 tttgaaacct caatttgaga aggttattca gactttaaag gaagatccct atctccctac
     1681 ccaatcattt gagaagttaa aacctacaca tgaaggtagg tatt
//
LOCUS       DACDYJ010000168         1572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDYJ010000168 DACDYJ010000000
VERSION     DACDYJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.175"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            45..104
                     /gene="ermCL"
                     /locus_tag="IWM51_002850"
     CDS             45..104
                     /gene="ermCL"
                     /locus_tag="IWM51_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HAP9972182.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            164..898
                     /locus_tag="IWM51_002851"
     CDS             164..898
                     /locus_tag="IWM51_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HAP9972183.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          588 a          248 c          211 g          525 t
ORIGIN      
        1 ttgtgctata attattctaa tttcataaaa aggagaatta atatatgggc atttttagta
       61 tttttgtaat caacacagtt cattatcaac caaacaaaaa ataagtggtt ataatggatt
      121 gttaatataa acattcatta taacctcata ggagtgggtt ataatgaaca aaaaaaatat
      181 aaaagatagt caaaacttta ttacttcaaa gcatcatata aatgaaattt tgagaaatgt
      241 acatttaaat acaaatgata atattattga gattggttca gggaaaggtc atttcacgtt
      301 tgaattagct aaaaggtgta attatgtaac cgccattgaa atagatccta aattatgtag
      361 gataactaaa aacaaactta ttgaatatga gaacttccag gttatcaata aagatatttt
      421 acaatttaag tttcctaaaa ataagtcata taagatatat ggaaatatac cctacaatat
      481 aagtacagat ataattcgaa aaattgtttt tgaaagcaca gctacagaaa gttatttaat
      541 agtggaatat ggatttgcta aaaggttgct aaatacaaat cgttcactag cactattttt
      601 aatgacagaa gttgatatat ccatattaag taaaatccct agagaatact ttcatccaaa
      661 acctagagtt aatagctcgt taattgtatt aaaaagacac ccttcaaaaa tatcactcaa
      721 agatagaaaa caatatgaaa attttgttat gaaatgggtt aacaaagaat acataaaact
      781 attttccaaa aaccaatttt atcaagcctt aaaatatgca agaattgacg atttaaacaa
      841 tattagcttt gaacaattct tgtctctttt caatagctat aaattattta atagataagt
      901 taaggggtgc ataaactgca tcccttaact tatcttattt tgcttaaaca atcttggccc
      961 gtaaactgca taactcatat gtaaatcgct cctttttagg tggcacaaat gtgaggcatt
     1021 ttcactattt ccggcaatta cttccatgta aagtataacc cactatactt tgcaagcaaa
     1081 gtgtgggctc gccgaggggt cttgcagacg gctctttcgg ctcttttgag ccgagtatca
     1141 gcgcaccctt atggtgcaat tagctgatac ctgtttgacg atttgctgcc ctaccttttc
     1201 ggtaggtatg caaacctcaa accaaaataa aaaccccctt cgacttcgtc agggggtttt
     1261 tatttatata aatctccgta aataatcgcg atttaataac atcttcaatt ttattttttt
     1321 gcgtatttta gaaatgtttt cactaaaaaa tatattatac aaaaaataca gtaatcgatt
     1381 ataattcttt gccagttaaa tatcatatcg ttattaaaat atcggaaaaa aatatcaaca
     1441 acaataaatg caaaagaaga ccataaaaat tgtttattac tcatacatac tctccatttc
     1501 tatcattttt aaatttacca gtcatctctc aaaaatctcc tagctatttt tcttttttaa
     1561 ccacataccg tc
//
LOCUS       DACDYJ010000169         1564 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDYJ010000169 DACDYJ010000000
VERSION     DACDYJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.176"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..359)
                     /locus_tag="IWM51_002852"
     CDS             complement(<1..359)
                     /locus_tag="IWM51_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP9972184.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSA"
     gene            complement(359..>1564)
                     /locus_tag="IWM51_002853"
     CDS             complement(359..>1564)
                     /locus_tag="IWM51_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9972185.1"
                     /translation="GMMGAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGI
                     MVRLDGSDGMINPLEIFATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQL
                     VSPQLKGTMKAEFKTYLNRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEF
                     LAFLGQLELPGASQEKKNRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDID
                     SITGLDRGVQQCQMYMAMTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHN
                     AINPDFIETVNYVTNFQREMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSS
                     TKIFMRMDESILRHIEGLVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKR
                     QLKLFDGGK"
BASE COUNT          449 a          345 c          267 g          503 t
ORIGIN      
        1 gccgacgtac caaacgctga ttcataaccg tattgcgaaa ggatcacact tggtaataat
       61 ttatacttca ctgctgcacc tacagcatat tgcgcaatct catcagaaaa agggcctctt
      121 cctttgattt ctttataaat gggtaagcct tccgaatatt ctcctgtaaa agcttcgcca
      181 ccactacttg aatcagaatt atcaatagaa cctgccgcaa gtattagaaa aagggtcaca
      241 aacacagtga tcaatatagt aatcaaacct gcagaagtac caaacaccca caaaaataag
      301 cgccgctttg cacgacgctt aacatactgt ttgattaaac tttcattttg gttttccatt
      361 attttcctcc atcaaacaat tttaattgac gtttgttcgg actaaaatga acacgaatgg
      421 tttcttttga gccaaaattg atcacagcgt ctccttgatt ttgttgagga atggattcta
      481 attctgagtt cgtcattgat ttaccaacta atccttctat atgacgtaaa atagattcat
      541 ccatacgcat aaagatcttc gtagaagaaa attcaaagac tttcttcagt ttcgagaagt
      601 tatcagaaga catattttct ggcaccattt cttgtgggga ttgtgtagct aaaacagtca
      661 ttgcttctac tttacgcatt tctcgttgga agtttgtgac ataatttacc gtttcaatga
      721 aatctggatt aatcgcatta tggcactcat caatcatcgc caagaagcgt ttgaagtctt
      781 gttcttccat ttgtttttct ttaattaagc gtcgttgtct tcgtccttga atcaatgcct
      841 gattccaaat caacgtcatg gccatgtaca tctgacattg ttgtacccct cgatctaggc
      901 ctgtaataga gtcaatgtca aaaaagacca actgttgttg gtcaaggtgt cgaatcgtac
      961 tatgaccatc aaaaatcatg ccataagtct cacataagga ttccacgata gactccattt
     1021 cttctttccg attctttttc tcttgggaag cccctggtag ttccagttgt cctaaaaagg
     1081 ctaaaaattc tttgaacgtc ggataagctt ctggatcttt ccctgtgact tgcgtatcag
     1141 tatttgaacg cgaatttgag gaagtaaaac cgcgtggtac tgttccatac tcttcataaa
     1201 aacgattcaa gtaggtcttg aattctgctt tcatcgttcc ttttagctga ggactcacta
     1261 actgtacctg atagactact ttgtctaagt gcgtttgata ggacgctgct tcattgatct
     1321 tcaaatcttt gactgaacca tcgtcatata aactggatgc ttcatcatac gtccgtgtgg
     1381 caaagatctc taaaggattg atcatcccgt cagatccgtc taaacgcacc ataataccgt
     1441 tatattcctt taagaaagga ataaagtctt ttcccttttc aaagccccaa ataaagtctc
     1501 ctgcagctaa atgggcttcc gcgatcattt taagaacggt cgatttacca gctcccatca
     1561 ttcc
//
LOCUS       DACDYJ010000170         1520 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDYJ010000170 DACDYJ010000000
VERSION     DACDYJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.177"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(86..736)
                     /locus_tag="IWM51_002854"
     CDS             complement(86..736)
                     /locus_tag="IWM51_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HAP9972186.1"
                     /translation="MPKVAVMAGTPIDTRMGADLLNEISCELLEVPISENPAQQTYFQ
                     TLPVFEKMHQIEVKIKDLQSKKIDLLLVYCNSLSASIDFDFLAKKYSMKIITPFQVYA
                     DLAQKHERIGVLTANGQGAAGIESVLVQHHSTIRVYNVTNLDWVHAVEAKIHPSIIVE
                     QFGLTDSMSFFMKNQVEAILIGCTHFPYFLTEYQSRTRIPCINPDDALIEKVKSIL"
BASE COUNT          511 a          270 c          211 g          528 t
ORIGIN      
        1 aaataaaaac acacattgat gcttatttga ctaaaaaata aaacaaaaaa atcctaagtt
       61 attgtctgtg tcaaaccaat gcttattaaa gtattgattt gactttttct attaatgcat
      121 catctggatt gatacaagga attctcgttc tggactgata ttctgtaaga aaataaggaa
      181 aatgagtaca accgatcaaa atcgcttcga cttgattttt cataaaaaaa ctcattgaat
      241 ccgtcagtcc aaattgttct acaataatac tagggtggat ctttgcttcc accgcatgta
      301 cccaatccag attcgtgaca ttataaactc gtattgttga atggtgctgc actaaaacac
      361 tttctattcc agccgctcct tgaccattag cggtcaacac accaatacgc tcgtgttttt
      421 gagccaaatc cgcatatact tgaaatggtg tgataatctt cattgaatat ttcttagcca
      481 gaaaatcaaa atcaatcgat gcactcagtg aattacaata aaccaacagt aaatcaattt
      541 ttttactctg tagatctttt attttcactt caatttggtg catcttttcg aagactggaa
      601 gtgtttggaa ataggtctgt tgagctggat tttctgaaat aggtacttct aacaattcac
      661 aggaaatttc gttcaataga tcagctccca tcctagtatc aataggtgtt cccgccatta
      721 cagccacttt aggcatactg atcattcctt caaaatgaat ttttgcctat tatactctaa
      781 aaatcaaatt tgaagaatag cgatccggaa gttaaactga aaattctaaa gtaattcttt
      841 gatttattta gattcaactt aacatcttta gtgtactaat aaaaaattcg ctcaatattt
      901 ctagctatac tttatatccc aatatacata agcttcccta tttttacagg ttacaagtga
      961 aaattaaact gcttatattg attgcattta actactaagg atgatgcttg ttgttagtat
     1021 gtcagaaaaa tccaacagta tagttttgtc agcacattca tcattctttc ctgtaaaaca
     1081 aaaagagagt agacctattc ttccacttca tcatcgttat ctaaaacagc aattttctgc
     1141 tgtaaaatag tcataaattt tttctagtag ccttctcttt aaactcatca taatctcaat
     1201 tatctttttt taagatttgc tcaacttttt ccctcttgat tattgcaatt cacaccttat
     1261 aaagttttaa actaatggga gacgtcatct agcttaactt atagtccctg ctccgtttaa
     1321 tctacacttg ttatcttgta ttattgataa aagatagact agttacggat tgaaattagg
     1381 ttgcaattct aaaaatccag tagtctaaaa tcatgcttat taatttacaa tatcttaaaa
     1441 aagggtttta acttaataac agaaaaaatt attcatgggc gaaaaaatga ttaggatggc
     1501 gtaaaagtta aagatcttag
//
LOCUS       DACDYJ010000171         1508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDYJ010000171 DACDYJ010000000
VERSION     DACDYJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.178"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1142)
                     /locus_tag="IWM51_002855"
     rRNA            complement(<1..1142)
                     /locus_tag="IWM51_002855"
                     /product="23S ribosomal RNA"
BASE COUNT          356 a          408 c          282 g          462 t
ORIGIN      
        1 tacgcactct ttaaatggtg gctgcttcta agccaacatc ctagttgtct gtgcaacccc
       61 acatcctttt ccacttaaca tatattttgg gaccttagct ggtggtctgg gctgtttccc
      121 tttcgactat ggatcttatc actcacagtc tgactcccgg atatgaatga atggcattcg
      181 gagtttatct gaattcggta acccgagatg ggcccctagt ccaaacagtg ctctacctcc
      241 atcattctca attccgaggc tagccctaaa gctatttcgg agagaaccag ctatctccaa
      301 gttcgtttgg aatttctccg ctacccacac ctcatccccg cacttttcaa cgtacgtggg
      361 ttcggtcctc cagtgcgttt taccgcacct tcaacctgga catgggtaga tcacatggtt
      421 tcgggtctac gactacatac tcaaacgccc tattcagact cgctttcgct gcggctccgt
      481 ctcttcaact taacctcgca tgcaatcgta actcgccggt tcattctaca aaaggcacgc
      541 catcacccat taacgggctt tgacttgttg taggcacacg gtttcaggtt ctatttcact
      601 ccccttccgg ggtgcttttc acctttccct cacggtactg gttcactatc ggtcactagg
      661 gagtatttag ccttgggaga tggtcctccc ggattccgac ggaatttctc gtgttccgcc
      721 gtactcagga tcctcctagg tgtcttccac atttcgtcta cggggttttt accctctttg
      781 actgactttt ccaagtcatt cgactatctg aaagaactac catattggag tcctacaacc
      841 ccaacaagca agcttgctgg tttgggcttt tcccgtttcg ctcgccgcta ctcagggaat
      901 cgaattttct ttctcttcct gcaggtactt agatgtttca gttctctgcg tctacctcga
      961 atgtgctatg tattcacaca atcgtaacat cctattaaag atgttgggtt cccccattcg
     1021 gaaatctctg gatcatagct tacgtacagc tccccaaagc atatcggtgt tagtcccgtc
     1081 cttcatcggc tcctagtgcc aaggcatcca ccgtgcgccc ttattcactt aaccttatca
     1141 accttacggt tgggttttga tccttcttct agcgatagaa gttagatcaa gaaaataagc
     1201 aattgaactt attaaaaaac tctatccagc tcacaaatca gcctgtacgg caaaaagatc
     1261 gatcgtctca taaaatcaaa gattttatgg ccgtccgcct attttgcttc aggctaaaca
     1321 atttgttcag ctttcaaatt caacgcggtg ttctcggttt gtattactta catttacttc
     1381 aaatatccag ttttcaatga acaaaagtat ttgagagtaa acctctcaaa actgaacaaa
     1441 gtaaaaacaa attgtgtagt ctccgtaata ttccttagaa aggaggtgat ccagccgcac
     1501 cttccgat
//
LOCUS       DACDYJ010000172         1501 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDYJ010000172 DACDYJ010000000
VERSION     DACDYJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.179"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            137..475
                     /locus_tag="IWM51_002856"
     CDS             137..475
                     /locus_tag="IWM51_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972187.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(680..1255)
                     /locus_tag="IWM51_002857"
     CDS             complement(680..1255)
                     /locus_tag="IWM51_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP9972188.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
BASE COUNT          464 a          311 c          256 g          470 t
ORIGIN      
        1 taaaaaaata atccaagaaa cattgaacct atggatccaa aagctaccca aatactatag
       61 ctaacccccc gctaccttca attttgcaga taaaacgctt tgggagaaaa tttcaaagac
      121 aacaggccat cctatcatgc ccgattataa acaaaaagct ggctattcct tttgctctga
      181 caaatttcaa ttgggaaagt tcacgaaact agcaggtgaa gaagtttcaa ctgttcggat
      241 caaagaatgc ccgcttcaaa tagaaacatt ggttcaacga atttcaacta gacaagactt
      301 tgctattgta gaacgtgaga tcgtagctat ttttgtagaa gaagggattt tacatgatcc
      361 aactcatatc aatgtggata aatggcaacc tcttatctat aaattcagag aatatacaac
      421 tgccaaccag tcgttaactt tgaattttag attccaagaa ttcaaagagt cataatgata
      481 aacatcgtta ggtataaata aaaaagactc gcagcacgag taaaaaacaa atagaagcta
      541 tacattagaa aggtcgactg tgaccctata gatgcagtat atgcagctta tgagcatttc
      601 ttctgttctc tttgtttttg ggatctgtgc tgaagtcttt ttcgtttttt gccgctcttt
      661 ctttgaaaaa tcgattatgt tataccttct ccattactaa ttcaggcatc tgctgagtaa
      721 tgatttttcg cgcaaaagaa atatctgtta cccaaggttc gatttgttct tcagtcaaga
      781 caagcggcat tcggtcatgg attggtgcga ctgtgcggtt aggttcagta gtcattatga
      841 ttgattctgc cattttttca tcctgatggc tacgataaaa accgccaata tacactattt
      901 tttccttatt tgaaaacata aatttgtctt tttcttcatt ccactcataa aagccactca
      961 ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg
     1021 tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat ccccacttca
     1081 tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca agatttgacg
     1141 gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc
     1201 gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca cacatcctaa
     1261 tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc aacaaaacgc
     1321 tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca gtgaatggta
     1381 ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgcttt
     1441 ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct gggtaaacgg
     1501 t
//
LOCUS       DACDYJ010000173         1499 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDYJ010000173 DACDYJ010000000
VERSION     DACDYJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.180"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            10..1449
                     /locus_tag="IWM51_002858"
                     /note="aph(2'')-Ia"
     CDS             10..1449
                     /locus_tag="IWM51_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAP9972189.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          638 a          108 c          255 g          498 t
ORIGIN      
        1 gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat agatgatgat
       61 tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta tggtggtaga
      121 gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg ggaagatgaa
      181 gtttttagag taattattga atataacaat gttcctattg gatatggaca aatatataaa
      241 atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat agtctatggt
      301 atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac aagatatatt
      361 aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat tttagaccct
      421 cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag aattattgaa
      481 gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt aatggaatat
      541 agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga gcattacttt
      601 gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag tgtggcatat
      661 ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa aaaaggttat
      721 gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa tgtaaaaatt
      781 cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta taaagaaatt
      841 aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga acaaaatttg
      901 ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta tacagatatt
      961 agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt gttgcgtgaa
     1021 actatttata atgatttaac tgatatagaa aaagattata tagaaagttt tatggaaaga
     1081 ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga ttttagttgt
     1141 aatcatctat tgttagatgg caataataga ttaactggaa taattgattt tggagattct
     1201 ggaattatag atgaatattg tgattttata tacttacttg aagatagtga agaagaaata
     1261 ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat tgagaaagca
     1321 aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt ttatggaatt
     1381 aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa aaggacttat
     1441 aaagattgat tatataatat atgaaaagct attataaaag acattagtat taaatagtt
//
LOCUS       DACDYJ010000174         1468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDYJ010000174 DACDYJ010000000
VERSION     DACDYJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.181"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IWM51_002859"
     CDS             complement(150..1337)
                     /locus_tag="IWM51_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP9972190.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          429 a          314 c          256 g          469 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact tttacacaat tatatggacg
     1441 taatcgtgaa tggatattta cttttaat
//
LOCUS       DACDYJ010000175         1456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDYJ010000175 DACDYJ010000000
VERSION     DACDYJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.182"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            108..1040
                     /locus_tag="IWM51_002860"
     CDS             108..1040
                     /locus_tag="IWM51_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP9972191.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="IWM51_002861"
     CDS             1112..>1456
                     /locus_tag="IWM51_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972192.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DACDYJ010000176         1442 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDYJ010000176 DACDYJ010000000
VERSION     DACDYJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.183"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(182..255)
                     /locus_tag="IWM51_002862"
     tRNA            complement(182..255)
                     /locus_tag="IWM51_002862"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(219..221),aa:Ile,seq:cat)
     gene            complement(444..1436)
                     /locus_tag="IWM51_002863"
     CDS             complement(444..1436)
                     /locus_tag="IWM51_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9972193.1"
                     /translation="MSLLTALFLLPINGFAYTINNEFNLGANEGSSQVANNQYILLHE
                     TANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQ
                     IELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGD
                     HTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNY
                     AHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYN
                     TSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          431 a          287 c          262 g          462 t
ORIGIN      
        1 taaaaatcaa agtaaaactt ttcaaatata cagaaatata actatgtgaa acatcctttc
       61 attaatccat aaaaggatac ataaaaaagc cactcatttg agtggcaatg aagaaaagct
      121 ttagcttgta taatactctt caaaaaattc taacacagaa cgattcaaat ggctacgtta
      181 atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg ctctgaccaa
      241 ctgagctaag ggtactggtt gttgccacat aaagccataa acaatcaacc agtagaatgt
      301 gtggcaacaa acctgttatc gcatagcttg gagtgtgact attatgggtg atagtgaaga
      361 tatgcgataa catcactatt ttatcgaatg atttttatag ttgtcaatat agttatgtaa
      421 tgctcctcaa cgaggagcta tttttatcgt ttaggaatat ttaaatacca acgtttgtca
      481 tggaaatctt gcgcaccgcc tttagtgttt ccctctggat cattcgttgc ccgcatcatg
      541 acatagactt tcttattagg gaagttacgc atgttaaaag atacatgata gccaacattt
      601 ccagaagtat tataagcttg atttacatct gatctataaa ttccatcagc tcttactcga
      661 gctaattctt tcccagtatt gtaatccata atgaagatat actcgtattt atagttagca
      721 atgtgccatc cagctacatg caaatttgca ttttcgattt ctccaaactg atcaatgtgg
      781 gcgtaattcg ttccatctgt cagtgtagga ttagctgcac cagctcgtgt tggatcaatg
      841 acaggcttgt tttcagaagt tgttggattt tcatcggtaa atccatgagc taaatcatat
      901 gctaattttt ctttacttac gcccatttca gaaagataac cgtaaggatc tgtatgatcg
      961 ccccagatgt tttgtgttac ccataaatgc gatttgattc ccggttggtt ataaggagtg
     1021 tccaacgtta atggaatacc atatttcatt gctgaatctc tagccaattc aacgtatgcc
     1081 ttgtagtttt tctcaaacgt tgctttatca tgtgtgtgtt gtaactcaat ctgcacagga
     1141 ctgttggcat tagcatacga accagcaccg tactgtacat aaccaggttg accgacttga
     1201 taaacaattc cgccgtctcc cacaatataa gcagtataag cgctagtcca tgaacgttgc
     1261 atatactgcg cttcattgcg tcctgttgct gtttcattag ccgtttcatg cagtaaaata
     1321 tactgattat ttgctacttg tgagctacct tcatttgcgc ccaaattaaa ttcattgttg
     1381 atagtatagg caaatccgtt aattggcaat aaaaaaagag ccgttaacag gctcatcgca
     1441 gt
//
LOCUS       DACDYJ010000177         1433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDYJ010000177 DACDYJ010000000
VERSION     DACDYJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.184"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            526..>1433
                     /locus_tag="IWM51_002864"
     CDS             526..>1433
                     /locus_tag="IWM51_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972194.1"
                     /translation="MNENGEFEESKKYQATSEEEKAAVRELLEEAVPTDFLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVREIEATPEDKKR
                     IESGQIEVIKKTYYTIEKVFDITQTQ"
BASE COUNT          593 a          225 c          315 g          300 t
ORIGIN      
        1 ttgggaaaat ccaaaagaaa attagtacga ttgcaaaatg aattaaacga acaaattgaa
       61 aagatgtttg aacaccagag aaagaccaac ggtcaaccaa tgaacgataa gagaaatggg
      121 cattcttggt ttaggcaaca agagcgaatc gaaaataaag tgcatagtct gagagaagag
      181 atcaagcaac aagaaaaaca agtagaaaaa cttgaaagac aagaagagat aaaggaaatg
      241 ggctataaca agtacggcgg actggatatg acgatcgaga atattccaag aatcaaagaa
      301 gaaatcgaac gctttgaaaa aggtgaatcg actttttcag ccgctacgat ccgaaaatac
      361 caacggaaat tggaaacatt agaacagctg aaagaaagat cggaaaaagg aaaagaaaac
      421 cttcttcctg aagtacaagc aatcattgac agcggacgag tgacgcaatg gaaaaaaaat
      481 ccgaccattt attttctgaa aggctatcga aaagtcgctt tagaactgaa tgaaaacggg
      541 gaatttgagg aatctaaaaa ataccaagcc acgtcggaag aggaaaaagc agctgttcgt
      601 gaattacttg aagaggctgt accaactgat tttttgaagg agaaagaaaa cgatcatttt
      661 tatcgagtag aattcaacga aaatgatcca gaaaaaggat tgaaaagtta tggtggggag
      721 atcgtaacat tcgaattgat cgatcaactc aaaacgttgg ataaccaata cgccgcagaa
      781 gcaggttatt ataaattcta ttttgaagaa gtgaagaacg gagaagtagt cgatcagatc
      841 cgaatggatc taggggatgg cgtcaacagt aatcaaccca tctatttaga gttagcaaaa
      901 ttgtgcgaac aagtacctga gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg
      961 aaagaacaaa tgcagtcgaa agaaacaaaa acaaaacaaa agggctatca aaaacgaacg
     1021 gcagaagaga ttaaacaaga agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa
     1081 aagcatactc attctcctga agacgtcaaa gaacttttga actttatgtc tcgttttcca
     1141 gaacgatcct ttagaaatca attattgatt gaggaacaat ttccaggtgc aacggcttgt
     1201 ttaggtcgtg ccgcgttgaa aaaagaagga atctatatca aaaaagggga acaagggaac
     1261 aagatctttg tcagaaaaac ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga
     1321 gaaatagaag ctacaccaga agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc
     1381 aaaaaaactt actacacgat tgaaaaagtg tttgatatca cacaaacaca gtt
//
LOCUS       DACDYJ010000178         1407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDYJ010000178 DACDYJ010000000
VERSION     DACDYJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.186"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            291..845
                     /locus_tag="IWM51_002865"
     CDS             291..845
                     /locus_tag="IWM51_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9972195.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            1099..>1407
                     /locus_tag="IWM51_002866"
     CDS             1099..>1407
                     /locus_tag="IWM51_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972196.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT          570 a          149 c          241 g          447 t
ORIGIN      
        1 ttcaaactta aatttcatat aatttaatta tgtaaattag ctattctaag aacttctacg
       61 gataactgcc cgtctaaaat ttaaataaga gcttagaatc ttcatataag taaggttaat
      121 ttttttgtac aaaaaagtcg gaatattctg agttactgaa atgatataaa atcataaact
      181 aaaccccttg attttcttca cttttaacag agtatatata taagttcatg attatggtaa
      241 aatatatata taatcatgaa ctcagaatat ttttaagaag ggaaacaata gtgagctata
      301 atgtacaacc gttaaggaca aatgaagaga tacaagattt tcagttttgg ctaagaagaa
      361 caagtaatcc agaaagagat agttttttat ttttgtttgg aatcaataat gggttacgta
      421 tgtcagatat tataggatta aaagttggag acatacgagg taaaagtaag cctattattg
      481 ttgaacataa aacaggtaag cgaaaaccga tttttataga taatttgaga gaagagattt
      541 tattatatac tgaaggtaaa gaagaaaatg attggttatt tccaagtcgg caacaaggtc
      601 gacatataac aagggatcgt gtctatcaga tatatgcaga tatagcagaa aaattaggaa
      661 gagatgatat aggaacacat acattaagaa aaacttttgg gtatcactac tacaagaaaa
      721 ctagagacat tgctacctta atgtttattt ttaatcatag ctcgcaagca attacaaaaa
      781 ggtacattgg tataactgaa gatgaaatag gggcatcgtt acgtggtttt aaattgggag
      841 gctaattgag atattaggat atattttttg tgtataatga aaacttagaa ggtgagatta
      901 atgaagataa ctgaattttc ggaagtattt aaaacgttta gtaacattta caatagaata
      961 ccagagatag aacaaaatgt tgtagttgat aatgattatt atatagagaa tgattttaat
     1021 aaaccaagaa aatatgacaa cctatacaat tttatacaca atagaacaac aataaataac
     1081 aacagtaaca ctcgagtttt gattaattca gcatattttc atattaaata tttaaagaaa
     1141 tatgagtatg aagaagttgt attagattat gggctaaata gacagtcatt tttaatttat
     1201 gcaataaata acggaaattt gccaagcaat aaaaagaaca taattattga aacatatgcc
     1261 tatctggagg ggggatctaa aaaactaatt aagtcatgta atatagagta tcaattatca
     1321 gtaggagaaa ttaaatgtgt tttcgaagaa gatttagaac ttgacttaaa aaaaattttt
     1381 gccagcattc atgattatat aggtttg
//
LOCUS       DACDYJ010000179         1364 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDYJ010000179 DACDYJ010000000
VERSION     DACDYJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.187"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IWM51_002867"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IWM51_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP9972197.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          393 a          242 c          216 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DACDYJ010000180         1334 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDYJ010000180 DACDYJ010000000
VERSION     DACDYJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.188"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            86..1165
                     /locus_tag="IWM51_002868"
     CDS             86..1165
                     /locus_tag="IWM51_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9972198.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          359 a          357 c          369 g          249 t
ORIGIN      
        1 agccatttgg aaataaaaaa acgccacgtg aattccatgt tatattgaag ttaccacaac
       61 tctcaataga caaggatgaa ttcacatgac gcaaaaccat gataacacag tttcacgcaa
      121 gggaaaacac ctaacctact cagaacgttg ccaaatcgcc atcctgaaaa aggaaaacta
      181 ttccaaccgt caggttgcgg atgtcctggg ctgcgtgccg cagaccataa acaacgaaat
      241 caaacgcggg accgttactc agcttaaccg ccagaatcag aacgggaagg tctacgacta
      301 ttatcatgac gtctacgacg ccgatgccgg gcaggcggcc tacgagaaga accgcttgaa
      361 ctgcggcaga cggcccttat gggccgacac ggacgccttc attgaatggg cggacgacat
      421 gatgctgttg gaaaaatggt ccccggacgt ggtggtgggc tttgccagaa aacacgaatt
      481 gttcgacccg gccattatcc ctgggaccac caccctctat cagtggatcg accgtggcat
      541 catgaaaacg aagaatatgg acctgctaga aaaactgtcc cgcaaaccga aatcgtcccg
      601 tcatggcaac cgcccgaata aacgggtgct agggaaatcc attgaagagc gcccggaggc
      661 ggttggtacc cggaaaacct tcggccattg ggaagtcgac acggtcgtag gaaacaagac
      721 caagtcggat gccgtgctgc tgacgttggt ggaacggcag acacgcttcg aggtcattct
      781 gaagctggcc gggaaggatg ccgaatcggt caaccaagcg gtccgacagc tgagagaacg
      841 ggctggtgcg tacttcggcc agctgttcaa gacggttacc tcggacaacg gctcggagtt
      901 cgcgggactg cacgatgcct tgcaggatgt ggcaaacatc tacttcagcc acccctatgc
      961 ttcctgggag cgcgggacga gcgaaaacca acacaagctg attcgccgtt tcatccccaa
     1021 gggcagccca atcggcaacg tgagcgaaaa ccaaatcttg cggattcagc gttggatgaa
     1081 cgattacccg cgaaagattt tgggctatgc aacgccgcat gacacgttcg tccaggcatt
     1141 taggcaggaa cggttgacgg cttgacacgt gggcccttga caacgagcct ccctcggcat
     1201 ggcttgtgtc tggaaagctg gccggggcca gccagtcggg agactaccat gcctcagctc
     1261 attgtcaagg gcccactcga ttagaaaaat tagacggttg gtggtaacga gcaaccgggt
     1321 caccatgggt ggct
//
LOCUS       DACDYJ010000181         1308 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDYJ010000181 DACDYJ010000000
VERSION     DACDYJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.190"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IWM51_002869"
     CDS             complement(<1..132)
                     /locus_tag="IWM51_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9972199.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..>1308
                     /locus_tag="IWM51_002870"
     CDS             542..>1308
                     /locus_tag="IWM51_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972200.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVT"
BASE COUNT          525 a          212 c          273 g          298 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaaacgga
      541 catggcagaa gaaaaagaac cactagacaa tagtcgcttg ggaaaatcca aaagaaaatt
      601 agtacgattg caaaatgaat taaacgaaca aattgagaag atgtttgaac accagagaaa
      661 gaccaacggt cagccaatga acgataagag aaatgggcat tcttggttta ggcaacaaga
      721 acgtttagaa aacaaagtgc atagtctgag agaagagatc aagcaacaag aaaaacaggt
      781 agaaaaactt gaaagacaag aagagctaaa ggaaatgggc tataacaagt acggcggcct
      841 ggatatgaca atcgaaaata ttccaagaat caaagaagaa atcgaacgct ttgaaaaagg
      901 tgaatcgact ttttcagccg ctacgatccg aaaataccaa cggaaattgg aaacattaga
      961 acagctgaaa gaaagatcag aaaaaggaaa agaaaatctt cttcctgaag tacaagcaat
     1021 tattgacagc ggacgagtga cgcaatggaa aaaaaatcca accatttatt ttttgaaaga
     1081 ctatcgaaaa gttgctttag aactgaatga aaacggggga tttgaggaat caaaaaaata
     1141 ccaagccacg tcggaagagg aaaaagcagc tgttcgtgaa ttacttgaag aggctgcgcc
     1201 aactgattct ttgaaggaga aagaaaacga tcatttctat cgagtagaat tcaacgaaaa
     1261 tgatccagaa aaaggattga aaagttatgg tggggagatc gtaacatt
//
LOCUS       DACDYJ010000182         1281 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDYJ010000182 DACDYJ010000000
VERSION     DACDYJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.191"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            151..1023
                     /locus_tag="IWM51_002871"
     CDS             151..1023
                     /locus_tag="IWM51_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9972201.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          394 a          228 c          290 g          369 t
ORIGIN      
        1 aaattattag aattttaaaa aatgataaaa attcaaaaag aaaaaatagt agttatttac
       61 ttgtcatgtt actaaataaa aaaataattt aaatatgata tatagtcatt tatttagtga
      121 tttcattcta aaataaaggg agaaatgtct atgctatacg gtgcaattga agcaggcggc
      181 acaaaatttg tgtgtgccat tggcgatgaa gaaatgacga tcaaggaaag agtgagcttt
      241 ccgacaacga cacctgagga aacaatgcct ttagtcattg acttcttcaa acaataccaa
      301 gcagatttag cagggattgg gattggctca tttgggccta tcgatatcca tcgcgattcc
      361 gccacttatg ggtacattac ctcgaccccg aaattggcat ggcaaaattt tgatttcatc
      421 ggtaccatga aaaaagaatt tccgatcccg atttcttgga caacagatgt caatgcggct
      481 gcctatggtg agtatgtctt cggcagtggc aaaggattga gcagtgtcgt ttactatacg
      541 atcggtacgg gtgttggcgg cggtgcctta caagaagggc gctttatcga agggtttagt
      601 catccagaaa tggggcatat gctcgtcgtc cctcatccaa aagatgactt tgctggcagc
      661 tgtccatttc atggcaactg tttagaaggg atggccgctg gacccgccat cgaaaaccgc
      721 ttagggaaaa aaggacaaga ggttgcagaa gatgatcctt attgggaaat cgaagcctcc
      781 tatattgctc aatgtgccta caatacaacc ttgatgtttt ctccagatgt gatcattttt
      841 ggcggtggtg tgatgaaaca acgccatatg ctgcaaaacg tccacgacgc atttgcaaaa
      901 ttggtcaatg ggtatgtcga aacacctgag ttaagcaaat atatcgtgac cccagcatta
      961 gaagacaacg ctggaacgct cggttgtttg gcattggctc gagaagcggt cttacattca
     1021 taaacgaaaa aaggctcttc gacatttagt gttggtagag aaagatacaa ctgaaaattt
     1081 agtcataacc agttaggcag agtgcttaac tggtttttgt tttacttgga agatcaagcc
     1141 ttgaatgaga taaattcttg ctcgaaagtt atggaaattt cgatagccgt aggccacttg
     1201 tattgtgcaa tagtttttta caaaaaaaag ggggtgcgga tatttttttg gaccttttta
     1261 acaaatatat ctttgcttct t
//
LOCUS       DACDYJ010000183         1220 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDYJ010000183 DACDYJ010000000
VERSION     DACDYJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.192"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            2..>1220
                     /locus_tag="IWM51_002872"
     CDS             2..>1220
                     /locus_tag="IWM51_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP9972202.1"
                     /translation="MITEVSYETLQKKKTLVVDAVLSPTPRACRSCGSTVVDGNGKAI
                     IVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRY
                     KIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKILPRVLMVDEFRSHA
                     SIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQLT
                     KRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAEKLKKNWRFLLKNR
                     ANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFFHELVEAFRDKDPD
                     LFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNGKIEAKNTHIKTMKRVS
                     YGFKSFENMRIRIF"
BASE COUNT          401 a          243 c          237 g          339 t
ORIGIN      
        1 gatgattaca gaggtctctt acgagaccct tcagaagaaa aagacgttgg tcgtcgatgc
       61 tgttctctcg cctactcctc gtgcttgtag aagttgtggt tctactgtgg tagatggaaa
      121 cgggaaagca attatagtga aaaatggaaa aaaggaaacg attgtccgtt ttgaacaata
      181 caatcatatg cctttggtta tgcgcctaaa aaagcaacgc tatacctgta aaaactgtcg
      241 cacccattgg acggctcaaa gttattttgt ccaacccaga cattcaatcg caaatcatgt
      301 tagatataaa attgcttctt tactgactga aaaagtatct ttatctttta ttgcgaaaag
      361 ctgtcaggta tctttgacca ccgttattcg tacattgaaa gagtttaaaa gctatttacc
      421 aaagcaatct aagaagatcc tccccagagt attgatggtt gacgaatttc gttcgcatgc
      481 ttccatagaa gataagatga gctttatttg cgcggatggc gaaacaggaa aattaataga
      541 tgttttgcct acgcgtaaat tacctcgatt aacaagctat ttcttaggtt gtaccaatcc
      601 agaagaagta gaattcttgg tgacagacat gaacgccgcc tacttccagc tcaccaaacg
      661 tgttctgcca aatgcgaaag tcgtgattga tcggtttcat attgtcaaac acatgaatca
      721 agcgttcaat gagttgcgta tccgtgaaat gaatgaactt cgtaaagccg gacagaaaag
      781 ccaggcagaa aaactgaaaa agaactggcg ctttttgcta aaaaatcgtg caaacatcaa
      841 ccattatgaa tacaaaacat ggaaaagttt ccgagcacca aaatacccat ttcttactga
      901 agcaatgatg attgatcgat tgcttgagtt ttctccgccc ctaaaggagg cgtatccctt
      961 ttttcatgaa ttagttgaag cctttcgaga caaagaccct gacttatttt tctccttatt
     1021 ggcagaactt cccgaaacgt tagatgacag ctttcgggaa aagcttcaaa accttctgac
     1081 ctatgaagaa ggcatcacca acgcaatgat ctatccttat tccaatggaa aaatagaagc
     1141 gaagaatacc cacataaaga caatgaaacg agtatcctac ggatttaaat catttgagaa
     1201 catgagaatt agaatctttt
//
LOCUS       DACDYJ010000184         1215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDYJ010000184 DACDYJ010000000
VERSION     DACDYJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.193"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            414..947
                     /locus_tag="IWM51_002873"
     CDS             414..947
                     /locus_tag="IWM51_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP9972203.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACDYJ010000185         1195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDYJ010000185 DACDYJ010000000
VERSION     DACDYJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.194"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IWM51_002874"
     CDS             <1..1076
                     /locus_tag="IWM51_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9972204.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DACDYJ010000186         1188 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDYJ010000186 DACDYJ010000000
VERSION     DACDYJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.195"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            141..398
                     /locus_tag="IWM51_002875"
     CDS             141..398
                     /locus_tag="IWM51_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972205.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IWM51_002876"
     CDS             complement(630..1043)
                     /locus_tag="IWM51_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAP9972206.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          453 a          151 c          148 g          436 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatcc
//
LOCUS       DACDYJ010000187         1123 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDYJ010000187 DACDYJ010000000
VERSION     DACDYJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.196"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>1123
                     /locus_tag="IWM51_002877"
     CDS             <1..>1123
                     /locus_tag="IWM51_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9972207.1"
                     /translation="YKGTLLYKPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTY
                     LELKKQRFKCKSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNIS
                     TSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVE
                     NRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIG
                     RTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYL
                     TESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLR
                     TFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFR"
BASE COUNT          334 a          229 c          229 g          331 t
ORIGIN      
        1 tttacaaagg aactcttctg tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc
       61 cctctaggat cattcgttgg gggacgacaa ccgttcggtt gttactgaat gatgtgtctg
      121 agtatcgcac ttatctggag ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa
      181 cattcgtagc ggatacctct gttgctgaaa aacactgttt cattagtcaa aaagttcgtt
      241 ggtctgtcat tgctcgctta aaagagaata cttccatgac ggaaatcgct cgtcagaaaa
      301 atatttccac ttcttcggtc tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt
      361 tcaaacagac tttgccaaag gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg
      421 ccagtgcaat gagttttatc atgatggacg gacaaagcca tgcattattg gatattgtcg
      481 aaaatcgacg cttgccttat ttagaacgct atttttctcg gttttcatta gcgactcgtg
      541 aagccgttca attgattgtt attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc
      601 ttttcccgaa tgctcagttg atcatcgatc gtttccacat cgtccaacat attggcagaa
      661 ctttcttgaa tcatcgagtg aaagaaacca cggtacgact taaaggaaat tcccactctc
      721 aacgagggtt agggaaaaaa ttgaagcgct attggaaact cctacaaaaa gaagaagcaa
      781 agttaaacta tgaaaaacgg gtttggcgtg ccagttttaa agattatttg actgaaagtg
      841 agattgccga tcgactgtta gctgcttgtc ccaacttacg ccaaggctat cagttatacc
      901 aagacattct ttatgcggta aagaaaagag atcagtcact atttgaggat tgtttaacta
      961 gagacattag cggtctgcca gaaacctata caaccacttt acgaacgttt aaaaagtttc
     1021 tgccacagat ccaaaatgcg cttcactaca gctattcaaa cggaccattg gaatgcttaa
     1081 acaaccacat caaagtatta aaacgcaatg cgtacggctt tcg
//
LOCUS       DACDYJ010000188         1115 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDYJ010000188 DACDYJ010000000
VERSION     DACDYJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.197"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..77
                     /locus_tag="IWM51_002878"
     CDS             <1..77
                     /locus_tag="IWM51_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP9972208.1"
                     /translation="EFLFPKFLEDSSELQDIYNYLYRR"
     gene            82..1029
                     /locus_tag="IWM51_002879"
     CDS             82..1029
                     /locus_tag="IWM51_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9972209.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          356 a          170 c          237 g          352 t
ORIGIN      
        1 tagaattttt gtttcctaaa tttttagaag attcttcgga gcttcaagat atctataatt
       61 atttatatcg gaggtaaaag gatgagctgt tatcttatca ccggtggggc tggcttcatt
      121 ggttctacct tagccaatca tttaggagaa gaacatgtgg taatcgtcgt cgatgactta
      181 tcaatgggaa aagtagaaaa tcttgatatg acgaaaaata tcacttttat tgagggagat
      241 gtcgctgaga aatccttgat ggaagagatc atgaaagcta ataaatttga ttatatcttt
      301 catttagcag cagttgctag tgtggcagat tcagttgccc gaccagtaga aacacatcgt
      361 gtgaattttg aaagtgtatt aatgatttta gaattgatcc gcaagtatca acctgattta
      421 aaacggatcg tgtttagttc ttcagcggct gtttatgggg atgaaccgac tttaccaaaa
      481 aaagaagagt ccgtgattcg cccattgacg ccttatgcgg ttgataaatt cgctgcagaa
      541 caatatgtgc tagattattg tcatttatat gatgtacccg gtagcgcagt tcgttttttc
      601 aatgtctatg gtccaaatca aaatccgaat tctccataca gtggtgtgat ttctattctc
      661 gtagatcgct ataagaaaca aatcgtagga gaagcaactt catttacatt atttggtgat
      721 ggaagccagt ctagagactt tgtttatata gatgatgtaa tccaagcatt acttttagtt
      781 gcgaatgagg aagctgcact ggggaaacaa ttcaatgttg gaacgggtaa agcaaccaca
      841 cttttggaat tgattcatgc aatcaatgaa attctcggtg tagaattgac acttgaatac
      901 aaagaagagc gttcgggtga tatccatgac tctctagcag atattactaa gatccagtca
      961 attggctatc aaccgaaata tggtatttta agtggaatga ctaattattt gaaaactgaa
     1021 atcaattaaa acatactaag ataagattag agggtaaatt caggctcttt gtcaataagg
     1081 actgatgagt tttgtaaaat caaatctggg ataga
//
LOCUS       DACDYJ010000189         1100 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDYJ010000189 DACDYJ010000000
VERSION     DACDYJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.198"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            54..1100
                     /locus_tag="IWM51_002880"
                     /pseudo
     CDS             54..1100
                     /locus_tag="IWM51_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180785.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          468 a          114 c          215 g          303 t
ORIGIN      
        1 tttggaaaaa taaaaaatta tatttcatca acgtttcata aaaggtgtgt agaatgttat
       61 tgaaagtagc tataaaatgc tattgtgtaa aaaataaagg aggaataatt tatgaaaaaa
      121 tagtaccaag aggagcatgc ctattacttg cattatgttt gagcttaagc gcagttgggt
      181 gtcaaaaaag taatgaaaac aataaaataa aagaaggaca atccataagt tctgaagagg
      241 aaggcatgtc aattaatgca aatggcaaaa atgaaacttt taagccatca gattatacat
      301 tggaggcaaa gaaagaatat gtttatgaat atttagggct taaatttaaa ctctcagaca
      361 aatttagaaa ttatatagct gataaaaaga tagcgatgtt agatgatcaa agtcctatag
      421 ataaagaact aaagtatgct attttgacat ttgagaaaat gacagaagaa caaaagaatg
      481 cagttataga aaagatgggc gatggatata aaaattggca aaatgagctt gaaagaattg
      541 ggactattgg tatatttgaa aaaaatacat cagaagaaaa aaatctaaaa ttataaaatg
      601 tgatactcat actaaaatag gagtttcaag tgatggaaaa tacgattgct atttaagcac
      661 aaatagcgga gctgaaagta atcttcttga tgaattaaaa agaactgaga ttcaaattat
      721 agataaaaaa gaacgtccta aaaatggttt tgtgctatca gaaaaaactg atttagaaaa
      781 tacagaagca tttaacaaag aatcggttaa agatttacgt aaactaccaa caaaggacat
      841 aaatggcaag gattttacaa gtaaagattt tgagaagtat gatcttacta tggtaaatgt
      901 atttgcaact tggtgtacag cttgtgtaaa ggaaattcct gatttggttg aggttcagaa
      961 tgaaatgaaa agcaagggtg taaatatcgt tggagttgtt acagatcctg ttgatgataa
     1021 cggtgaaaac aaggaagcaa ttgaaaagtc aaagttaata catgaaaaga caaaggcttc
     1081 atatcctttc ttaatgccag
//
LOCUS       DACDYJ010000190         1091 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDYJ010000190 DACDYJ010000000
VERSION     DACDYJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.199"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..980
                     /locus_tag="IWM51_002881"
     CDS             6..980
                     /locus_tag="IWM51_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP9972210.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          384 a          135 c          187 g          385 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta a
//
LOCUS       DACDYJ010000191         1078 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDYJ010000191 DACDYJ010000000
VERSION     DACDYJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.201"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..525
                     /locus_tag="IWM51_002882"
     CDS             <1..525
                     /locus_tag="IWM51_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9972211.1"
                     /translation="SEKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTI
                     SNEINQYREREPEIQPAEVKQEHKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRT
                     LHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKN
                     EIYYEQQLSQKERV"
     gene            586..>1078
                     /locus_tag="IWM51_002883"
     CDS             586..>1078
                     /locus_tag="IWM51_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972212.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRD"
BASE COUNT          422 a          165 c          234 g          257 t
ORIGIN      
        1 tcggagaaag ctattcctta tattgcagca tggacgaaga atggtcagac gttggatgat
       61 aaggaagcgg cggaacgtgg gaaaatcatg aatgatgtca gccgtgtcgc aaatgaattc
      121 attcaaacca tttctaatga aatcaatcaa tacagagaaa gagaaccaga gatccaacca
      181 gcagaagtga agcaggagca taaaaatact gtagaaacgg cagagaataa gaatattgtg
      241 cccgttggtt cactttggat cgacaagaaa gacgggaaag taatggaaga agatacgggt
      301 cgaacgttac atttgaagga tgcggcgttt attgaagatc cgcagcataa tcaagtgatt
      361 ttaaaaggaa aaagttatgg aaaagagatt caaacggcgt taccttcaga agaatttgcg
      421 ctaggaaatc cattccgatt ttcacttgaa acagtgtcag aagtggccaa acgaaaaaat
      481 gaaatctatt atgaacaaca attgtcacaa aaagaacgcg tataaaacaa gtaaaaaaga
      541 ctatctgaaa aacggatagt cttttttctt ttgggaggtt tgaaaatgac cgaaaaaaaa
      601 atgagtctta ttgatcgatg taaacagatc gatatagtgg attttgctcg aaacaatgga
      661 atggctgtag tcaataaagg acgagactat cgcttagaag accatgattc atttgttttt
      721 gatcgaagaa aacaacgttt ttattggaac agtcaaaata ttagtggcga cattattgaa
      781 ttagctaaac tttttttcat agacaaagag atccaagact ctaagcaaca atttaaagca
      841 gccacagatt ttattctaaa gaatgaagac aaaacagaac gagtagaaaa tcttcatttt
      901 gagacagaaa agtacaaaga tcaccccgtt gattatcaac cactcacaga aaaaggaagg
      961 aattatctga aggaagaacg aaaactacca gagtggctga ttgattatgc agaaaaagaa
     1021 gggttgattg ccgaactaaa gccaaaacat gaacggcaaa atttcttagt tcgagacg
//
LOCUS       DACDYJ010000192         1062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDYJ010000192 DACDYJ010000000
VERSION     DACDYJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.202"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(36..992)
                     /locus_tag="IWM51_002884"
                     /pseudo
     CDS             complement(36..992)
                     /locus_tag="IWM51_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          347 a          178 c          159 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cattcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca ac
//
LOCUS       DACDYJ010000193         1060 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDYJ010000193 DACDYJ010000000
VERSION     DACDYJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.203"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(277..732)
                     /gene="tnpA"
                     /locus_tag="IWM51_002885"
     CDS             complement(277..732)
                     /gene="tnpA"
                     /locus_tag="IWM51_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP9972213.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
BASE COUNT          295 a          209 c          180 g          376 t
ORIGIN      
        1 attatttctg cgccagcatt ttctaatgtt tgaatttgtg ttttgtaatc ttggttgctt
       61 gtgctaattc ttgaatagcc gtaaattgcc aaaatttcat caccctttct ggacataagt
      121 ttctgacacc agttaaaatc aagtctatca gtaacccata cacccgtgag gacgggtgta
      181 tttttaatcc ccctagaagg ggtcgacact cgctgcccct taaaagggga tttttaaaag
      241 accgccaact gctcatcttc actcacttac ttctctttat tttttaaatg gttctacgta
      301 ctctctttta cttataatat atcgaatgcg atcatcagct tcttgttcac ggatgtattt
      361 ttcatttgtc ttttgattca atccgactgt actcacatag tatctcttcg accaaaagga
      421 tttgtttccg tatttatatt tgagatttgc gtgctgctcg tggattagta ccgcactacg
      481 acctttcaga taccccataa aactggatat acatattttt ggtggtactc gaacaagcat
      541 gtgaatatga tctagcattg catgtgcttc tatgatttcc acatctttca tttcgcacaa
      601 cttcctaagt atttttccaa gatcttttcg taattgccca tagattactt ttcgttgata
      661 ttttggtgta aaaataatat gatacttaca attccaacga gtatatgcta aacttttatc
      721 gtcatttgac atgaaaaaac tcctttcgat ctgatgagcg gttggtagcc actcactagt
      781 atatcgaaag gagtttttat cgctaaagat acttcttcca cgcgtagaaa cgcgcggttt
      841 tcttgtttcg cacgctttac gcacaaaact gactgaagtc attaagaaaa taaaagggtc
      901 taaaactttt aagttttgta ccgaccccca aaagttagac caaaaaatct aacttttgga
      961 ggtcggtatt ttttatggct aagtatactt ttgaacttaa attaaagatc gttcatgatt
     1021 acttggatgg taaaggcgga agtgattatc tggcaaagaa
//
LOCUS       DACDYJ010000194         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDYJ010000194 DACDYJ010000000
VERSION     DACDYJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.205"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1035)
                     /locus_tag="IWM51_002886"
     rRNA            complement(<1..>1035)
                     /locus_tag="IWM51_002886"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACDYJ010000195         1013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDYJ010000195 DACDYJ010000000
VERSION     DACDYJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.206"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            66..>1013
                     /locus_tag="IWM51_002887"
     CDS             66..>1013
                     /locus_tag="IWM51_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9972214.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLS"
BASE COUNT          349 a          173 c          188 g          303 t
ORIGIN      
        1 taaaaaagtc atttgtgcta cactcaaaat agttccgacc aaagaattat aaggagtgag
       61 tacaaatgac ctatacccat cttacaacgg atgaacttgt aataatagag tcttatttca
      121 aaatgaatca atctgttgct aaaactgccc attgcttgaa tcgttcaaga caaacgatcc
      181 ataaagtata tctgttcttc aagcaaggaa aatcagcctt agaatattat caacagtata
      241 agaaaaacaa atcaaactgt ggtagacgtc cgcttgtttt acccgaggaa caatcagaat
      301 atattcaaag aaaggttgtt caaggatgga cacccgatgt gattgttggt cgtgcagcgt
      361 ttcctattag ttgttctgct cgtaccattt atcgtatgtt caaaaagggg ctatttgatt
      421 cttctgactt accgatgaaa ggcaagcgta aaccgaatgg acatcaagaa agacgtggaa
      481 aacaaacttt ccgccgctct attcatgaac gtgaaaagga ttatagccaa ttctcaaatg
      541 agtttggtca ccttgaaggt gacactatcg taggtctgaa acataaaagt gctgtaatta
      601 ccttagttga acgattatca aaagttatca tcacattgaa accgtgtggt agacaagcga
      661 ttgatattga aaaaaaatta aatcaatggt ttgaatctgt accgaaaaac ctattcaaat
      721 ccatcacttt tgattgtgga aaggaatttt caaattggaa acagatcagt aatgtcaatg
      781 atattgccat ttatttcgct gatccaggaa cgccgtctca aagaggccta aacgagaatt
      841 ctaacggatt gttacgtaga gatggtttat tgaaatctat ggatttcaat tcagtagatg
      901 aattttttat tcaatctgtc gcatctaaac gaaataatat tcctagaaaa tcactggatt
      961 atcgaacacc tttggaagta tttttgagtt acgtaagtat tgatgatctg tct
//
LOCUS       DACDYJ010000196         1000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDYJ010000196 DACDYJ010000000
VERSION     DACDYJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.207"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            369..1000
                     /locus_tag="IWM51_002888"
                     /pseudo
     CDS             369..1000
                     /locus_tag="IWM51_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DACDYJ010000197          931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDYJ010000197 DACDYJ010000000
VERSION     DACDYJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.208"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            75..>931
                     /locus_tag="IWM51_002889"
     CDS             75..>931
                     /locus_tag="IWM51_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9972215.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIVVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKMV
                     YQASSKEVA"
BASE COUNT          289 a          189 c          211 g          242 t
ORIGIN      
        1 gtaaatagaa aaaaggaaat cccttctgta gaatagagtt accacaaaac attcacagaa
       61 gagaggactt ccctatgaac gattttacta cagaaattct aaagactcta gcgaacaaag
      121 gcgatttgaa tgaattattc cgtgtccatt tggaaaaagc agtcaatacg cttctcaaaa
      181 cggagttaac ggctttccta gattacgaaa agtatgatcg cattggtttt aacacgggta
      241 attctcgtaa cggctcctat gaccgtacgg tcaagaccga gtatggggaa cttcatctcc
      301 agattccgcg cgaccgcaat ggcgagttca agcaacagac tgttcctgct tatagacgga
      361 cgaatgacac gttagaggag accgtcattc acctcttccg aaaaggtatt accatgtcgg
      421 aaatcgcaga cttgattgag aaaatgtatg ggcatcacta cacgccacaa accatgtcca
      481 atataacaaa atcatttaca gaagaggtaa cggcgtttaa agggcgagag cttcatgacc
      541 gttatgctgc tatttatatg gacgcaacgt atattccgtt aaagcggaaa accgtcgcca
      601 aggaagctat tcatatcgta gttggcattc gcccggacgg atcaaaggaa gtattgagct
      661 atgcgattgc accgactgaa tccatcacga tttgggagga aattttatta gaccttcaag
      721 agcgcggttt gaaaaatgtc ctcctgttca tcacggatgg cttaaagggg atggtaggag
      781 cgattagtcg gttctatccc aaagctcgtt ttcaacattg ttgtgtacac gttttccgta
      841 atatcagtca caaagtgcgt gtcaataatc gtaaggaagt ctgtgatgat tttaaaatgg
      901 tgtatcaagc ctcatctaaa gaggtggcgt t
//
LOCUS       DACDYJ010000198          929 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDYJ010000198 DACDYJ010000000
VERSION     DACDYJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.209"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..354
                     /locus_tag="IWM51_002890"
     CDS             <1..354
                     /locus_tag="IWM51_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9972216.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
     gene            355..903
                     /locus_tag="IWM51_002891"
     CDS             355..903
                     /locus_tag="IWM51_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972217.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
BASE COUNT          376 a          129 c          198 g          226 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacagagtag aggaagaaca
      541 aaaaaagttg gcgcaccaat cagaaagttt gataaggttt attccaccaa gcatccttta
      601 aaactgtcga ctttgttaaa tgcgacttcc agagttttag tagaaaagta tgagactaat
      661 atgacaagag atgaattgct tagaaaagct ttagatgagt atattaaaca gaatcttacg
      721 caagaagata agcaagattt gtttaatgac gtggtgaaag aactagactt gttcagagaa
      781 aaatatccaa ttgttccaga attagatgat cgtggagaaa taataagaac taccagtcag
      841 ataaaacaag ataccgaaga ctcactgcgc aaagggtggg gaatgaataa taaagttaga
      901 taataatcaa agaagaacta agtaactac
//
LOCUS       DACDYJ010000199          880 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDYJ010000199 DACDYJ010000000
VERSION     DACDYJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.210"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            465..797
                     /locus_tag="IWM51_002892"
     CDS             465..797
                     /locus_tag="IWM51_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9972218.1"
                     /translation="MSYTGILSFEDICHYGKRCTATEKITKKLSTGQNKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
BASE COUNT          303 a          140 c          171 g          266 t
ORIGIN      
        1 tatgcttgtg atgaattgat tttcgttctg cacttttgga cgataaattg tgataattga
       61 aaatacaaaa aaaagaaagg cccctgtaag gctcgggaga ctggtaatct ccgtcagtgt
      121 cctacaggaa agcattaaga tgacgcctta atgcgtgccc ttcagtgtta cggtattacc
      181 cgcaacggac tcagtataac agatattcaa aagtgatacc actttttttg gctctattaa
      241 ggttaaaaat acgtgatatt gtaatgaatc tatgcctaag cataatgtta taaaaatgtc
      301 cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg tttgaatgcg
      361 ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa aaataacaag
      421 aaaaacttat taaaaatatt taaagaaagt gagaggatta ctttatgtca tatacaggaa
      481 ttttgagttt cgaagatatt tgtcattacg gtaaacggtg taccgctaca gagaaaatca
      541 cgaagaaact atcaactggc caaaataaaa ccgttgtgca gtgtaagaaa tatataatcc
      601 agaaagacaa agtctcagaa gaaatgattt attacattgg gaaacaaaag caaataattt
      661 taaaggatcc tatcccttta aaagagctat atccaaccat caaacatgtt tatgaccaaa
      721 acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca aaagggatgg
      781 ggcgtttgat cagctgaatt gatcattctc ataacttgta ttttatcgtt tttcgcttgc
      841 ttgaaaatat tgtcgagact tctaaaggaa cttagaatca
//
LOCUS       DACDYJ010000200          866 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDYJ010000200 DACDYJ010000000
VERSION     DACDYJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.211"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..793
                     /locus_tag="IWM51_002893"
                     /pseudo
     CDS             1..793
                     /locus_tag="IWM51_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          311 a          161 c          152 g          242 t
ORIGIN      
        1 cacgccccaa accatgtcca acatgactaa agtgctgact gaagaagtaa atgcatttaa
       61 agttagaact ctaaatgata agtatgtcgc tattttcatg gatgctactt atattccttt
      121 aaaacggcaa accgtctcca aagaagcaat ctatattgcc attggtatac gagaagacgg
      181 cactaaagaa gtactaagtt atgcgattgc tccgactgaa tcgacctacg tttggaatga
      241 gttgttacag gacatttact ccagaggggt tcatgatgtc ttactgttta ttaccgatgg
      301 cttaaaaggc atgaaagata cgattcacca gatttatcct aaagctaaat accaacattg
      361 ctgtgttcac gtttcccgta atattgctca taaagtacgt gtcaaagatc gaaaagaaat
      421 ctatgatgat tttaaggctg tttatcaagc tagctcaaag gaagaggcaa ataccttttt
      481 agggagtatg attgagaagt agcaaaaaac ttatcctaaa gtgacgcagt cactgataaa
      541 aaatcaagat ttattgactt tttatgagtt tccgcctggt atccgccgaa gcatttactc
      601 aactaattta atcgaatctt tcaagaaaca aatcaagaaa tacagccgca gaaaagagca
      661 gtttcaaaat gaagaatcac tagagcgttt cctagtatcc atctttgata catacaatca
      721 aaaattctta aatagaagcc ataaaggctt ccaacaagtg acggatacat tagcttcaat
      781 gtttactgag taacccatta ttttgcagaa ggacgagtta tttacacaaa attattgacg
      841 ctcccaataa aaaaacaaac aaagta
//
LOCUS       DACDYJ010000201          854 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDYJ010000201 DACDYJ010000000
VERSION     DACDYJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.212"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            81..587
                     /locus_tag="IWM51_002894"
                     /pseudo
     CDS             81..587
                     /locus_tag="IWM51_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            580..>854
                     /locus_tag="IWM51_002895"
     CDS             580..>854
                     /locus_tag="IWM51_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972219.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVG"
BASE COUNT          302 a          132 c          182 g          238 t
ORIGIN      
        1 tcatcgtcat tcctcctgct atctttttct attattctac cttatctgat agtagattta
       61 aaactttgca acagaaccgt aattcacaag tgttaccacg agattatact agaagaaatg
      121 aaatcgagct ggtggtaaaa gaaatggcgg aacaagtagc cattcgtatc aggcagcata
      181 attgtaaaac aggatgtgtt catttaaaca ttggtacgtc gatcttagaa acgagaccag
      241 gattttcaca ccagatgaaa atacctatca cagataatac aaaggaattg cagaattatt
      301 gtcttttctt gtttgataag tattatgaag gacaagaagt aaggcacgtt ggaataacct
      361 atagcaagct tgtgtacacc gattcgcttc agctggattt atttagtgat ccacaaaagc
      421 aaattaacga agaaaattta gataaaatta ttgacaagat tcggcaaaaa tatggtttta
      481 cgtcgattgt acatgcttct tcaatgttag aaagcgctag aagcattact agaagcacac
      541 ttgttggtgg tcatgcgggc ggaaatgggg gaattaaaaa tgattgatta caatgatcgt
      601 ggacgtttaa agtgggcagg gttttattta tctgatcata ctgaagaaat tgatgccgac
      661 aatgaacaac gaaccagtca aaatttagca aaagaacaaa tgacgactga agagattgcg
      721 gaagtattga acgctgcagt cataaaaaat agatcagtat ctattcaaaa agaagaacga
      781 aacgctgaag gatactatcc agaagatatt gttggcaaga tcttaggtag tgatgagctg
      841 gggatctacg ttgg
//
LOCUS       DACDYJ010000202          852 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDYJ010000202 DACDYJ010000000
VERSION     DACDYJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.213"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>852
                     /locus_tag="IWM51_002896"
     rRNA            <1..>852
                     /locus_tag="IWM51_002896"
                     /product="23S ribosomal RNA"
BASE COUNT          239 a          187 c          256 g          170 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat gg
//
LOCUS       DACDYJ010000203          815 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDYJ010000203 DACDYJ010000000
VERSION     DACDYJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.214"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(68..409)
                     /locus_tag="IWM51_002897"
     CDS             complement(68..409)
                     /locus_tag="IWM51_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9972220.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            complement(396..725)
                     /locus_tag="IWM51_002898"
     CDS             complement(396..725)
                     /locus_tag="IWM51_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972221.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
BASE COUNT          245 a          178 c           81 g          311 t
ORIGIN      
        1 actacctttt tattaatcgt cgtgactcct tctaagcctt tatctgtaaa atttattcct
       61 ccagttttta ttcttcttta tccgctacaa attcaatgat ttcaacgctg acaatataat
      121 cactcaaata tgaccaagta tcttcaacag ataattcgtc tccattatca ttcataagcg
      181 tcattttaaa ccattcatta ccttcatcat cttcaggtag caagccgaat ccaattggat
      241 tatcagtcaa tttttgatcg tataaatcat cctcgtctaa tgctaaaaca tctaatcctt
      301 tacaatttcc gcctcttcta aacgtcacaa atcctttgta atcccaacgt tggaaagaaa
      361 ccttaatcgt atgtgtgccc aatcgtatat tcggatcata atttttcatt tgttgtaacc
      421 tcctcataat cagttagtac tataaaggta tctttgtttt tatccaaaaa atctaataaa
      481 tcttttgtag aagatatctc ttcatcaact tcaccataat aaataccttc gataattgtt
      541 gctatatctg aaaaactaat ttttactgga catttatcgt ctaccgcatt caataccgac
      601 aaaattgctt ctttttctgc aatctctcgt ttgaatttct ttggagctaa tcgcatgaca
      661 ctcaagtcat tttcaagctt ttcaatccat gcagttttac tcattttcgc aaatttttga
      721 atcattcctc taccaccttt gctatctact aaattagttt tacttcataa cgaatatctt
      781 tatcgttttt cgttcactca aaaatatata aaata
//
LOCUS       DACDYJ010000204          791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDYJ010000204 DACDYJ010000000
VERSION     DACDYJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.215"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..782
                     /locus_tag="IWM51_002899"
     CDS             <1..782
                     /locus_tag="IWM51_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9972222.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          242 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc t
//
LOCUS       DACDYJ010000205          784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDYJ010000205 DACDYJ010000000
VERSION     DACDYJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.216"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            97..336
                     /locus_tag="IWM51_002900"
     CDS             97..336
                     /locus_tag="IWM51_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972223.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            422..>784
                     /locus_tag="IWM51_002901"
     CDS             422..>784
                     /locus_tag="IWM51_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9972224.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDA"
BASE COUNT          302 a           98 c          162 g          222 t
ORIGIN      
        1 aaacgaaaga aaaagataac ccaagactga agtgtagagc gtatgtgatc ataggctttt
       61 ttatttttgc aaaaaacaag gaaggtagga aaactcatga attatgaaga aatcgaaaac
      121 agaaaaaaag tgtcgaaaga aatggaagaa aagttgctaa aaacgatgaa acaaaaacac
      181 cttaaaagat tatctgtcat gcaatatatc aatgatatgc agataacagg aaaagagaaa
      241 gcttgtttgt taggttcaat gaaaaacttt gaacagttga gacgaactta tgtgaaaaaa
      301 agttctaatt gtcaactatt gttagaagtc tcatgacatt cattaataga attcattttt
      361 aaaacttttt ctatggaaat gagaaaaaga ttttttgttt tgtctgaaag ggggtagctg
      421 gatgagaaaa tggttatttg gttttttagg agtcgcttta atcgtctttt gcagtgtgtt
      481 tggctatgtt agctaccaaa aacatgaagg aaaggtgttt aagcaaaata tagaaaaaaa
      541 gatgcccgta gatcaaatca atgaacatgc aaagagttat aaggaggatg caacgaacgt
      601 caataatgat atgtctttgg ggcaaatgtt gtccatacaa aaagaagcaa tcgaaatggg
      661 tgtcaataaa caagcctttg cacaaattca gatacctgca cttggtttag cattgccaat
      721 ttttaaaggg gcaaatcaat acactttgag tcttggagcg gctacttatt tttatgaaga
      781 tgcc
//
LOCUS       DACDYJ010000206          772 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDYJ010000206 DACDYJ010000000
VERSION     DACDYJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.217"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(15..>772)
                     /locus_tag="IWM51_002902"
     CDS             complement(15..>772)
                     /locus_tag="IWM51_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HAP9972225.1"
                     /translation="TVKLLATVGEEVGELGGEQLTKAGYVDDLDALIIGEPTNYSLMY
                     THMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHLAETIENPVLGKT
                     IHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVNELNQETDYHLEL
                     MIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKANHSFDFVVFGPG
                     VVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          201 a          152 c          160 g          259 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tc
//
LOCUS       DACDYJ010000207          755 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDYJ010000207 DACDYJ010000000
VERSION     DACDYJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.218"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..461)
                     /locus_tag="IWM51_002903"
     CDS             complement(<1..461)
                     /locus_tag="IWM51_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9972226.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          248 a          138 c          107 g          262 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaag
      541 atatatatgt actaccttat aattaataag taaaaggtag tacataaata aggaaaagct
      601 gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt atcgagaagc
      661 agagaatact cgcgacttta gtcatgtaga cgaatcagat tttcgataaa aaagaagccg
      721 tccttcttaa gaacgacaac aaatcactag attaa
//
LOCUS       DACDYJ010000208          742 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDYJ010000208 DACDYJ010000000
VERSION     DACDYJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.219"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            51..731
                     /locus_tag="IWM51_002904"
     CDS             51..731
                     /locus_tag="IWM51_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP9972227.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPA"
BASE COUNT          266 a          125 c          148 g          203 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccacgatt aaaggcatgg aagccattcg aggattatat aagaaaaccc
      661 gaaaagaagg cactctcttc gggttttcgg tctgtactga aatcaaggta ttattgggaa
      721 tcccagctta aatcatagat ac
//
LOCUS       DACDYJ010000209          735 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDYJ010000209 DACDYJ010000000
VERSION     DACDYJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.220"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            101..631
                     /locus_tag="IWM51_002905"
     CDS             101..631
                     /locus_tag="IWM51_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972228.1"
                     /translation="MISDNKWEINLKRFISEYIYTIETKSSFSLNEDSDPLKKYTIQC
                     KKNKDEKLFVIRPDLMKSNTHYIKEKPKDCDYIIISEANKTVFLIELKSSAQTSPAKE
                     ISEQIDGGYKWLQHLSFLTKYDFTEFSYKIIKVCCKFEARQDRKNLFKPNEYGIYNMN
                     GPKLDLRELYRKCNDL"
     gene            complement(626..>735)
                     /locus_tag="IWM51_002906"
     CDS             complement(626..>735)
                     /locus_tag="IWM51_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9972229.1"
                     /translation="NIVKDLNEGVSISKIAKNYNVTRQTIYRIKKDLNL"
BASE COUNT          296 a           86 c          111 g          242 t
ORIGIN      
        1 aaatttctaa catcagttat tatgataatt ttgatgatgt caattatgat ttacaggaaa
       61 gatataatga tttaatcgaa gaattagagg agagaggaga atgatttcag ataataaatg
      121 ggaaataaat ctcaaaagat ttatttcaga atatatatat acgattgaaa ctaaatctag
      181 tttttctctt aatgaagaca gtgatcctct aaaaaaatac actattcaat gtaagaaaaa
      241 taaagatgaa aaattgttcg taataagacc agatcttatg aagagtaata cccactatat
      301 aaaagaaaaa cctaaagatt gtgactacat catcataagt gaagcaaata aaacagtatt
      361 tctgatagaa cttaaaagct cagctcagac ttcccctgca aaagaaatta gtgaacaaat
      421 tgatggtgga tataagtggt tacaacatct tagttttcta acaaaatatg attttactga
      481 attcagttat aaaattatta aggtatgttg taaatttgag gcaagacaag atcgtaaaaa
      541 tctttttaaa cctaatgaat atggtattta taatatgaat ggtccaaaat tagacttaag
      601 agagttgtat agaaagtgta acgatctata aattcaaatc ttttttaatt ctatatattg
      661 tttgtctagt aacgttataa ttctttgcaa tctttgaaat agagacgccc tcattcaagt
      721 ccttaacgat gtttt
//
LOCUS       DACDYJ010000210          731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDYJ010000210 DACDYJ010000000
VERSION     DACDYJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.221"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            156..485
                     /locus_tag="IWM51_002907"
     CDS             156..485
                     /locus_tag="IWM51_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9972230.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            475..>731
                     /locus_tag="IWM51_002908"
     CDS             475..>731
                     /locus_tag="IWM51_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9972231.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHE"
BASE COUNT          278 a           83 c          146 g          224 t
ORIGIN      
        1 ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt cagcaaggac
       61 gcattacgag tcacagtaat tacttgtaaa tacattatct cgtggtataa tgtatttact
      121 aagtaattac atactgttag aaatgaggga tttaaatgat tacaaaaaca agaaaacaag
      181 gcaattcaat catgttgaca gtacctaaag attttaatgt tcctaatgga gtcgaagttg
      241 aagcaaagtt ggtagagaat ggtattcttt atgaatttgt tgaaccacaa aaagaatttt
      301 atgattttag cgaagatatt ttgtctgata taattgcaga aggctacgat aaagatgaga
      361 ttcttgtaga atttaagaat agaaaaaaca aaatgcattc ttcctttaga gatattgctg
      421 aagatacact tactaattct aaagtaatga ctaaagagga gttggcaaca gaaattggct
      481 tatgaaatcg ttccttcaaa acatgtcatt aagtatttaa aaaagctaaa agagaagcca
      541 ttgaaagaaa aatttttgaa tttaatttat gatgaaattg cgattgatcc atataagggt
      601 gaacaaaaaa ctggggattt atcaggtatt tggtctagtg ggttcaaata tgcgggcaca
      661 acatatagag tagcttacga gattatagaa aacaaggtta ttccagtatt gttatgtgga
      721 acgcatgaaa a
//
LOCUS       DACDYJ010000211          696 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDYJ010000211 DACDYJ010000000
VERSION     DACDYJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.222"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(54..629)
                     /locus_tag="IWM51_002909"
     CDS             complement(54..629)
                     /locus_tag="IWM51_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9972232.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DACDYJ010000212          695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDYJ010000212 DACDYJ010000000
VERSION     DACDYJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.223"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>695
                     /locus_tag="IWM51_002910"
     CDS             <1..>695
                     /locus_tag="IWM51_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAP9972233.1"
                     /translation="DKIKNRRNTDQLKQRDTVIIACVIWGIINGYTSQRATYRAVCSV
                     LFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNR
                     QAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEE
                     ANLPNILGDKGYISHKIHEKLALKGITISVPPRKNMDKSEKLDHSLLGKQRKTVETVF
                     SSLEKLGCQNFNS"
BASE COUNT          245 a          129 c          120 g          201 t
ORIGIN      
        1 agataagatt aaaaaccgaa gaaatactga tcaactgaag caacgcgata cagtgattat
       61 tgcttgtgtt atatggggca taattaatgg ctatactagc caaagagcca cgtatagagc
      121 ggtttgttcc gtcttatttc ctaatggtga ctttcctagt aggagtcgat tcactcgctt
      181 aagttcaaac ttagcttaca ctatcaagat tattcgatac tttttcatca agaagctcac
      241 caaaggtgaa ctagtcggaa ttatagatag ttttcctagt ccattatgta aaccagttag
      301 aaatagacaa gcaaaactat tgaatcaaat agccaaagtt ggctataact caacaaaaaa
      361 atcttatttt tatggtctta aaattcatat gatcgttact aagactggct ttccgattac
      421 ttactcaatc acgaatccag gtgtccatga cgtgaaagtg ttagagactt tatcggaaga
      481 agcgaatctt cccaatatcc taggggacaa aggatatatt agccataaaa tccatgaaaa
      541 gcttgccctt aagggcatta ctatatccgt tccgccacgt aaaaacatgg ataaatcaga
      601 aaaactagac cacagcttac ttggaaaaca aagaaaaaca gttgagactg ttttttcttc
      661 cttagaaaaa ttaggttgtc aaaatttcaa ctcac
//
LOCUS       DACDYJ010000213          692 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDYJ010000213 DACDYJ010000000
VERSION     DACDYJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.224"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>692
                     /locus_tag="IWM51_002911"
     CDS             <1..>692
                     /locus_tag="IWM51_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9972234.1"
                     /translation="KSVGKNGWQIVDQYEENIAYIELLQFHEGKARIDFNPNKIGQFL
                     ASSMKNFIHDLFLEPHFSRADIACDMVNVPDEFIMQYRVVDPVSFKPIYGRSGKLESA
                     YWGSRASERQIRLYNKKLEQETKRKVVPKEVETWWRLEMQLRRGKATDWHNMVQESLN
                     SFASLHFLPIDTTPTDRIMLAGLMSDQNFWSLLSRNSKYKYRELLKEESQNDELTNHL
                     RETFAESADNLK"
BASE COUNT          226 a          113 c          156 g          197 t
ORIGIN      
        1 caaatcagtt ggtaaaaatg gctggcaaat tgtcgaccaa tacgaagaaa atatcgcgta
       61 tattgagctc ttgcaatttc atgaagggaa agcacgaatt gattttaatc ccaataaaat
      121 tggtcagttt ttagccagtt cgatgaaaaa tttcattcat gatttgtttt tagaacccca
      181 tttttcgcgg gcagatatcg cttgcgatat ggttaatgtt ccggatgagt ttattatgca
      241 atatcgtgtg gttgacccgg tatcgttcaa accgatttac gggcgttctg gaaaactaga
      301 atcagcttat tggggtagcc gtgccagcga acgacaaatt cggctataca ataaaaaatt
      361 ggaacaagaa accaaacgaa aagttgtacc aaaggaagtt gagacttggt ggcggttaga
      421 aatgcaacta cggcgtggca aggcaaccga ttggcataat atggttcaag aaagtttgaa
      481 tagttttgcg agcctgcatt ttttgccgat tgatacaaca cctacagata gaataatgct
      541 cgctggttta atgtctgatc aaaatttctg gtctcttttg tcgaggaatt caaaatataa
      601 atatcgggaa ttgctgaaag aagaaagtca aaatgatgaa ttgacgaatc atttgcgaga
      661 aacgtttgca gaatcggcag acaatttgaa ac
//
LOCUS       DACDYJ010000214          651 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDYJ010000214 DACDYJ010000000
VERSION     DACDYJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.225"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(366..>651)
                     /locus_tag="IWM51_002912"
     CDS             complement(366..>651)
                     /locus_tag="IWM51_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9972235.1"
                     /translation="KIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHA
                     KWSGGRIQNWAMVLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          212 a          113 c           99 g          227 t
ORIGIN      
        1 gtatgggcta ctctgtgata cttattagcc attaaactat tttacccaca tagcatatat
       61 tttgaaatcg ttcattccgt aaattcaact aaatatgcct aaaaaaataa aaaatctaag
      121 atagaataag atttattcag tcttagatgg atttatagca actttttcgt aacaacttta
      181 aaaaatacga aaagaaagag ctgttttact ctgactacgt tgctaaattg tcgtttaatc
      241 attcatttaa accaaatctt tttttataga attcgtttta atcctcctga atccaaaaac
      301 tatgaaaacc gtaattaaat agttaagagt ttttaaccac cataattctc tcggctattt
      361 tgaaatcaga taaattttga tcggacacat agttgaataa acctaccgtt gttacggaag
      421 gtaaatcgca tacttttcat tctagaggag aaggtctcgt tgatcattaa ctgattaagc
      481 accatagccc agttttgaat tcgaccacct gaccacttcg cgtgtaattc tttcgtgcgt
      541 aaataaagta gttttaggag tgcattctca ttagagaacg ctcctttttt tgtgactttt
      601 ctgaagctgg agtggacact ttcaaccgca ttggtagtgt acataatttt t
//
LOCUS       DACDYJ010000215          650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDYJ010000215 DACDYJ010000000
VERSION     DACDYJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.226"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..515
                     /locus_tag="IWM51_002913"
     CDS             <1..515
                     /locus_tag="IWM51_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972236.1"
                     /translation="IPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEI
                     NQYREREPEIQPAEVKQEHKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLK
                     DAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYY
                     EQQLSQKERV"
BASE COUNT          253 a           95 c          145 g          157 t
ORIGIN      
        1 ctattcctta tattgcagca tggacgaaga atggtcagac gttggatgat aaggaagctg
       61 cggaacgtgg gaaaatcatg aatgatgtca gccgtgtcgc aaatgaattc attcaaacca
      121 tttctaatga aatcaatcaa tacagagaaa gagaaccaga gatccaacca gcagaagtga
      181 agcaggagca taaaaatact gtagaaacgg cagaacaaaa gaatattgtt cctgttggtt
      241 cactttggat cgacaagaaa gacgggaaag taatggaaga agatacgggt cgaacgttac
      301 atttgaagga tgcggcgttt attgaagatc cacagcataa tcaagtaatt ttaaaaggaa
      361 aaagttatgg aaaagagatc caaacggcgt taccttcaga agaatttgcg ctaggaaatt
      421 cattccgatt ttcacttgag acagtgtcag aagtggcaaa acgaaaaaat gaaatctatt
      481 atgaacaaca attgtcacaa aaagaacgtg tataaaacaa gtaaaaaaga ctatctgaaa
      541 aacggatagt cttttttctt ttgggaggtt ttaaaatgac cgagaaaaaa atgagtctta
      601 ttgatcgatg taaacagatc gatatagtgg attttgctcg aaacaatgga
//
LOCUS       DACDYJ010000216          597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDYJ010000216 DACDYJ010000000
VERSION     DACDYJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.227"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(310..>597)
                     /locus_tag="IWM51_002914"
     CDS             complement(310..>597)
                     /locus_tag="IWM51_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972237.1"
                     /translation="ETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNK
                     NYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          206 a           98 c           90 g          203 t
ORIGIN      
        1 attgtgagat gataataaag aagagtttaa agcgcacccc aagccacttc cccataagtg
       61 tgttacgctt taaactcttt tatatttgaa gctattaaaa ggcataccat atttttgaaa
      121 aaaagtgaga aaaaaggctt acaattggag tggtagttaa ttagtgactt atttttgatt
      181 ttatagcact gatactataa aatatagata tcatcatatt acacaatctt aatactaact
      241 taaaaaatat ctcctttcac aagtatggtg ataaaattcg ttccgggcta cctttttagg
      301 tagcctactt taatctttgt atctttctgg atcaacgaaa gtatacttta tatagtcata
      361 acgccgatga tcgctacgtg cgtccggcac gtcagtcacg atatcaaaca aaaagtatac
      421 atccttcttc attctagttt tcgcagcagg aattttgaaa tagttcttat tagaatagta
      481 gagattgatt aataagctat cttcgattgc taaaaagaaa acttctgaat tccatacttt
      541 ataaaaatct ttgataaatc tattcgaagg gtcaaattta aaccataatt gtgtctc
//
LOCUS       DACDYJ010000217          591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDYJ010000217 DACDYJ010000000
VERSION     DACDYJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.228"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(316..579)
                     /locus_tag="IWM51_002915"
     CDS             complement(316..579)
                     /locus_tag="IWM51_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAP9972238.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          224 a          103 c           78 g          186 t
ORIGIN      
        1 cttccttttt tgtatggatt ttttcattta cacaaaatat tttatactct cctgaatttt
       61 ttcatgttga aaacatcact aatcaaaggc aaattataat ttagaaatag aatgaaaaca
      121 ctaaatgaaa atagaacatt agaaaaaata gaatcaaaaa aacccaagac tagtgccgaa
      181 ataagggaaa caatcgaagc tacaaaaaat aatgatttcg ttgaacagga ccaatttatt
      241 tgtttccgat atttctcaaa aagaactttc tttgggaaag ttaaaggcga ctgtaaagtc
      301 gcctttagtc cttttttacc aagctgctgc atatttagct ccatattttt taattaatga
      361 tttggctgta gcaacaatac cagctgtaat taatccgcct ccaacaactg cagcaacaat
      421 agaaatgatt gtagcaaccg tgctaccagt attaattatg tcaataactt ttttagctgc
      481 ataagaagaa attccaatgt tagcacttag cattggtaca ccaacaaaga atcccgcaaa
      541 acctaaaatt ggtaaaacta ataataaatt ctttttcata ataattcctc t
//
LOCUS       DACDYJ010000218          541 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDYJ010000218 DACDYJ010000000
VERSION     DACDYJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.229"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..198
                     /locus_tag="IWM51_002916"
     CDS             <1..198
                     /locus_tag="IWM51_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP9972239.1"
                     /translation="DKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKL
                     ANEQRETFNELPTVSTENWLE"
BASE COUNT          208 a           64 c           79 g          190 t
ORIGIN      
        1 gacaaaggat tatatatgac tttactgaaa gcttatcaaa aacaaaaaac tgaaaaagta
       61 aacaatttgc agaatctaat tttcgtttat gtaaaaaatt ggtttgtcga aaaagctatt
      121 gcggcaaagc ttgcaaacga acaaagagaa acatttaacg aattaccaac agttagtacg
      181 gaaaattggt tagagtagtt gataaaaagc aattactata aaaatgatca attttaaatt
      241 agaacttttt tatgatttta attttattac aaatattaca aataaatagt tagtattccg
      301 gagtttttag attaaaagtt tatgaaaaca atcacaatcg taggttttaa attcataatc
      361 gttagatatt ttagttattt ttcactcaaa aaaataggga actagtgttt gcatatgtgc
      421 ttttttgagt tataataatt catgaagaga ggtaattcac ttatttcccc aagctgaaaa
      481 gcctgatatt acttcttttc aggcagtgct gctaatattc aattacactt ttttgtatat
      541 t
//
LOCUS       DACDYJ010000219          537 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDYJ010000219 DACDYJ010000000
VERSION     DACDYJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.230"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>537)
                     /locus_tag="IWM51_002917"
     CDS             complement(<1..>537)
                     /locus_tag="IWM51_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAP9972240.1"
                     /translation="YQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNK
                     TVEQENSTINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIR
                     PYPIFNHGETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYA
                     RNLYHGLHDQLEEKNEENM"
BASE COUNT          160 a           93 c           85 g          199 t
ORIGIN      
        1 catattttct tcgttttttt cttctaattg atcatgcagt ccatgataga gatttctagc
       61 atattcatct ggtaggaaat aatcataatc aatttgtaaa gccttaagat ccaagtccgc
      121 atgtgtacaa tgtatgtcta tatcattgat atcccacgat ggattgaaat cttcaatata
      181 ttgataagca taaggaagat tcgtttcccc atgattaaaa ataggatacg gccgtatttt
      241 atgacctttc aaatcttgtc gttttaaagg agcgagtaca atcgtttcac cttctaaaaa
      301 agtagaaatt ctttcaggag ttaagatacg atctgaatca acattgcgat tgatatgagt
      361 attcatacct aatgaattga tggtactgtt ctcttgttca accgtcttat tccccacttt
      421 tttagaaaat tcttcgatgg tattatcatc tgtcgacata atataaatat gtgtctgaca
      481 attttctttg ataatcgctg cgttttgttc tccatacttc gcataaagtt gctgata
//
LOCUS       DACDYJ010000220          530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDYJ010000220 DACDYJ010000000
VERSION     DACDYJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.231"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          180 a           79 c          123 g          148 t
ORIGIN      
        1 aacttggggt ttagtatttc gttcaaaaag ggaacaaaaa atacaagcat ttctcataat
       61 aatcgtgagt taactgatag ccaaaaaatt aatagtttgt ctggtagaaa ataattagca
      121 gatttacaag tgggaaataa aaagctgaaa gaagaaaatt gcatttcaaa ctacattttc
      181 tacaataact ctaaaatggc atattttgcg ttctgagcgg ttttagcgtt tatttcgttt
      241 agttatcggc acaatcggtg aaacacgtta gaggagcgaa aatggagcct gtaaggcgac
      301 tggtagcgga acggtcgtgt tagattgtgt agccgatagc tgaacgaaat aaatgctggc
      361 agcgccattc tatttcggca ggaggaggct caaggggttc ggggaatgaa attccctgag
      421 gtctttttat tttttctaga ggagtgctat aatcaaagaa aatgagaaaa aagagtaaaa
      481 aaatagtcga gcagtgttga cgcactctcg acctttacgg aaactacttc
//
LOCUS       DACDYJ010000221          529 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDYJ010000221 DACDYJ010000000
VERSION     DACDYJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.232"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            33..>529
                     /locus_tag="IWM51_002918"
     CDS             33..>529
                     /locus_tag="IWM51_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP9972241.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          194 a           98 c           87 g          150 t
ORIGIN      
        1 gacaaccaat tcacagaaaa gaggacttcc ctatgaatga ttttactaca gaaattgtgc
       61 aaactctagt cactaaaggc gatttaaatg aattattccg ttcgcactta gaaaaagcga
      121 taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca
      181 ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat
      241 atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt
      301 taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa
      361 aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata
      421 ctccacaaac catgtccaac atgactaaag ttctgactga agaagtaaat gcctttaaat
      481 ccagagcctt aaatgataag tatgtcgcta tttttatgga cgctactta
//
LOCUS       DACDYJ010000222          518 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDYJ010000222 DACDYJ010000000
VERSION     DACDYJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.233"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..330)
                     /locus_tag="IWM51_002919"
     CDS             complement(<1..330)
                     /locus_tag="IWM51_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9972242.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
     gene            complement(330..>518)
                     /locus_tag="IWM51_002920"
     CDS             complement(330..>518)
                     /locus_tag="IWM51_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HAP9972243.1"
                     /translation="LMVHRITFDQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGI
                     NQSEISALFSYIKEGASS"
BASE COUNT          143 a          105 c           71 g          199 t
ORIGIN      
        1 attatttttt gctgtttcaa aatgaatcga caaaacatat atttcatcat cggttaaatt
       61 accgatactt ctaactattt catcagaaat agttatggct tctttggata cttctttgaa
      121 caaatcccga tcgactacgg gaatcgtttc ctttctttga gaacgtttca gcatctcatt
      181 caagtgatta attagaatgg tccactgcaa ctcggttggt acaatagcat gttctactaa
      241 tcgctcttca attagatcaa tcacttgttt taacttttcc cgattttgac tttcctcgat
      301 aatttttaaa gcctcgtctg acattttcac taacttgacg ctccttcttt tatatatgag
      361 aataatgcac tgatttctga ctgattgatt cctaaattat tttctgcaaa aactgcttcg
      421 atttttccta ccagtgttcc tttgtctaaa ataatctggt ctacttgacg accttctgcc
      481 atatattgtt gatcaaatgt gatacgatga accattaa
//
LOCUS       DACDYJ010000223          509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDYJ010000223 DACDYJ010000000
VERSION     DACDYJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.234"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          229 a           70 c           81 g          129 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaaactt caaaaaagtt cttgacaaat
      361 aacaaacgat aagttatgat aaatgagttg ccgaaagatt tcgaaaaacg aaatcgaaaa
      421 aaacttttaa aaaagagttg acaatcgctt gtctctctga tatgatataa aagttgctac
      481 ggcagccaaa aatgaatcgg gaaacactc
//
LOCUS       DACDYJ010000224          504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDYJ010000224 DACDYJ010000000
VERSION     DACDYJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.235"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          230 a           64 c           43 g          167 t
ORIGIN      
        1 atgaataaaa tttagtttta aaattaaaga taattaagta ctaccagtgt caaaacaaaa
       61 aataaattaa gaactacctt atgcatataa aacatactac cattactagc aatatatact
      121 accttatttc agtagtatat attatattat tcttataaga caaggcttta ggttagaaaa
      181 caatagatta tatactacta aaaaatacaa taagtactac caatgtcaga tatatataat
      241 aattatgtac taccattaaa attaacttat ttaataagat actactgtgt taatctgaaa
      301 aaaattactt taaagtagtt acatactacc tgttaattta gatactacca ttaatactta
      361 aattagatac tacctatatt tatattaagt actaccgttc taagaataag ataataaaaa
      421 aatactacca aattgcaatt aaatactact taaaatgtaa aaaataagaa ctacccaaat
      481 taggtagttc ttaataagag aata
//
LOCUS       DACDYJ010000225          463 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDYJ010000225 DACDYJ010000000
VERSION     DACDYJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.236"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>463
                     /locus_tag="IWM51_002921"
     CDS             <1..>463
                     /locus_tag="IWM51_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAP9972244.1"
                     /translation="RKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSC
                     GEHKDAVLVKKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCP
                     FDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYE"
BASE COUNT          148 a           78 c           92 g          145 t
ORIGIN      
        1 tcgtaaattc ttaagagaca acccattaga agcgatcgta acagacttga cttatgtacg
       61 agtcgggaaa aaatggaatt atgtctgttt cattttggat ctgttcaatc gagaaattct
      121 cggctattct tgtggagaac ataaagatgc cgttctagta aaaaaagcat ttagccgtat
      181 caaacaacct ctgacagagg ttgagatttt tcatactgat cgtggaaaag agtttgataa
      241 ccaagctatt gatgaattat taacaacttt tgacatcaat cgatcattga gtcataaagg
      301 ctgtcctttt gataatgccg tagctgaatc aacttataag tcgttgaaag tagaatttgt
      361 ctatcaatac acatttgaaa ccttacaaca attggatttg gagttatttg actatgtcaa
      421 ttggtggaac caccttcggt tgcacggtac acttggctac gag
//
LOCUS       DACDYJ010000226          454 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDYJ010000226 DACDYJ010000000
VERSION     DACDYJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.237"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          151 a           65 c           73 g          165 t
ORIGIN      
        1 tgcgagcagc aactagaata atttaaacat tattaaactg tctatattgt tactaataat
       61 tcccttctta tagtgcattt tcttagaaaa aaacaatatt acgatatcat gatattaaga
      121 tatcgtaata ttatacaatt gtgattgata ttatatatgt ttttttattt ttttatcatg
      181 tttaatcttg aaggaatcat attctttttc taaagacagg agcttttcat aaacatcaga
      241 catgtttaaa ctagaggcca ggcttaaatc ataaacgaat gaagcaggta atgattttat
      301 tctacgttgt cgggttatcc agcttgcaac cgttccttgt gggtaggcat ataactcaca
      361 aaattgttct acagtgattc ctaaattctt gataatgtaa acattgatcg ggtgcgagta
      421 aacaaatgta ttgatagaca tggtgcgtaa gcct
//
LOCUS       DACDYJ010000227          442 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDYJ010000227 DACDYJ010000000
VERSION     DACDYJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.238"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>442)
                     /locus_tag="IWM51_002922"
     rRNA            complement(<1..>442)
                     /locus_tag="IWM51_002922"
                     /product="23S ribosomal RNA"
BASE COUNT          104 a          139 c           95 g          104 t
ORIGIN      
        1 taagtcctcg atcgattagt atcagtccgc tccatacatc actgtacttc cactcctgac
       61 ctatctacct gatcatctct cagggatctt actttcttaa agaaatggga aatctcatct
      121 tgaggtgggc ttcacactta gatgctttca gcgtttatcc cttccctaca tagctaccca
      181 gcaatgccct tggcagaaca actggtacac cagcggtaag tccatcccgg tcctctcgta
      241 ctaaggacag ctcctctcaa atttccaacg cccgcgacgg atagggaccg aactgtctca
      301 cgacgttctg aacccagctc gcgtgccgct ttaatgggcg aacagcccaa cccttgggac
      361 cgactacagc cccaggatgc gacgagccga catcgaggtg ccaaacctcc ccgtcgatgt
      421 ggactcttgg gggagataag cc
//
LOCUS       DACDYJ010000228          387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDYJ010000228 DACDYJ010000000
VERSION     DACDYJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.241"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..205
                     /locus_tag="IWM51_002923"
     CDS             <1..205
                     /locus_tag="IWM51_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9972245.1"
                     /translation="KVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQI
                     RQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          116 a           80 c           89 g          102 t
ORIGIN      
        1 caaagtagaa ttcgtgcatc aataccaatt tgagacactg gcacagctac gtctagaatt
       61 gtttgattat gtgcattggt ggaactatct acgcttacat ggcacgttgg cgtatgaaac
      121 accgatccaa attcgacaac agagattggc gaagcgaatc cttgataatg agcgcggatc
      181 tgatacctct ggagaggcag cgtaattgaa tgattgtgct tctgccggag aaaatcagat
      241 ccgaggatgc tcattgtcaa gggcaatcgg agccataaca ccgcagcatt cacaacacct
      301 tataattttt gtcaaaaaaa gtgttgccat tccactagtc taacttttgg ggtgcacatc
      361 actttgaggg gcggtacata aaaattt
//
LOCUS       DACDYJ010000229          352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDYJ010000229 DACDYJ010000000
VERSION     DACDYJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.242"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            68..>352
                     /locus_tag="IWM51_002924"
     CDS             68..>352
                     /locus_tag="IWM51_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP9972246.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNC"
BASE COUNT          125 a           58 c           73 g           96 t
ORIGIN      
        1 aaaacatatc gttctctagt atgattaaat cacgacaaaa taaacaactg agaggacgat
       61 atgctatatg aatgattcta tcaaaaaaat gctgcgatta atagagaaag atttgatgat
      121 tacagaggtc tcttacgaga cctttcagaa gaaaaagacg ttgatcgtcg atgctgtttt
      181 ctcacctgct ccccatacct gtagaaattg tggttctact gtggtagatg gaaacgggaa
      241 agtaattgta gtgaaaaatg ggaagaagga aacgattgtc cgctttgaac aatacaatca
      301 catgcctttg gttatgcgct taaaaaagca gcgctatacc tgtaaaaact gt
//
LOCUS       DACDYJ010000230          344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDYJ010000230 DACDYJ010000000
VERSION     DACDYJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.243"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..>344
                     /locus_tag="IWM51_002925"
     CDS             6..>344
                     /locus_tag="IWM51_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9972247.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKN"
BASE COUNT          128 a           30 c           58 g          128 t
ORIGIN      
        1 agtttatgga agaagtttta attgatgata atatggtttt tgatattgat aatttaaaag
       61 gatttcttaa tgataccagt tcatttgggt ttatagctaa agaaaataat aaaattatag
      121 gatttgcata ttgctataca cttttaagac ctgatggaaa aacaatgttt tatttacact
      181 caataggaat gttacctaac tatcaagaca aaggttatgg ttcaaaatta ttatctttta
      241 ttaaggaata ttctaaagag attggttgtt ctgaaatgtt tttaataact gataaaggta
      301 atcctagagc ttgccatgta tatgaaaaat taggtggtaa aaat
//
LOCUS       DACDYJ010000231          336 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDYJ010000231 DACDYJ010000000
VERSION     DACDYJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.244"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            121..327
                     /locus_tag="IWM51_002926"
     CDS             121..327
                     /locus_tag="IWM51_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9972248.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          120 a           43 c           81 g           92 t
ORIGIN      
        1 ataaaattga acaactggac gggcaaattg atgccttgct ttcagaaaga gaaatattga
       61 tagaagaaaa caagcgattg aaaaagaaag tagcccaacc aatgaaggga ggtggatagt
      121 atgaacaata gaacctttga tattttgaaa tggatcgcta tagtagttat ccccgcttta
      181 gcgacttttg tcggtcttgt cggaaaaggt gttaactggc ggtacacaga tataacggtg
      241 atgattatta caggttttgg agcatttcta gggagtattt taggtgtatc aaatcgaacg
      301 tataagaaaa tttcatcaga taaataggag gataga
//
LOCUS       DACDYJ010000232          335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.245, whole genome shotgun sequence.
ACCESSION   DACDYJ010000232 DACDYJ010000000
VERSION     DACDYJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.245"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(87..168)
                     /locus_tag="IWM51_002927"
     tRNA            complement(87..168)
                     /locus_tag="IWM51_002927"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(132..134),aa:Leu,seq:tag)
     gene            complement(176..248)
                     /locus_tag="IWM51_002928"
     tRNA            complement(176..248)
                     /locus_tag="IWM51_002928"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(213..215),aa:Lys,seq:ttt)
     gene            complement(252..324)
                     /locus_tag="IWM51_002929"
     tRNA            complement(252..324)
                     /locus_tag="IWM51_002929"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(289..291),aa:Val,seq:tac)
BASE COUNT           94 a           94 c           74 g           73 t
ORIGIN      
        1 ggctaaagga cttgaaccct cgaccctctg attacaaatc agatgctcta ccaactgagc
       61 taagccggca tgtaaaactt aaattatgcg ggtgaaggga cttgaacccc cacgccgtaa
      121 ggcgctagat cctaaatcta gtgcgtctgc caattccgcc acacccgcaa aaatatgagc
      181 cgtacagggc tcgaacctgt gaccctctga ttaaaagtca gatgctctac caactgagct
      241 aacggctcat atggaggtta acgggatcga accgctgacc ctctgcttgt aaggcagatg
      301 ctctcccagc tgagctaaac ctccatggat aactt
//
LOCUS       DACDYJ010000233          323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDYJ010000233 DACDYJ010000000
VERSION     DACDYJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.246"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..302)
                     /locus_tag="IWM51_002930"
     CDS             complement(<1..302)
                     /locus_tag="IWM51_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP9972249.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DACDYJ010000234          270 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDYJ010000234 DACDYJ010000000
VERSION     DACDYJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.249"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(41..220)
                     /locus_tag="IWM51_002931"
     CDS             complement(41..220)
                     /locus_tag="IWM51_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972250.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
BASE COUNT           96 a           54 c           37 g           83 t
ORIGIN      
        1 aggattcata gagataactc tatagaatcc gtaaaatacc ttatatatat aacgggaaaa
       61 tataaaacct aaaattatga taacctgctg cagcttctac atagaaagag aagcgctcaa
      121 caccaacctc atttatcaaa cgttggccat aaccccaaat ctcttctgat agctgatcaa
      181 aggttatata ctttagctca tattgctgga taatgttcat tctaatcgac tccatttatt
      241 atcctggtaa aattcgatat gacgtatttc
//
LOCUS       DACDYJ010000235          266 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.250, whole genome shotgun sequence.
ACCESSION   DACDYJ010000235 DACDYJ010000000
VERSION     DACDYJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.250"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(36..>266)
                     /locus_tag="IWM51_002932"
     CDS             complement(36..>266)
                     /locus_tag="IWM51_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9972251.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           78 a           39 c           47 g          102 t
ORIGIN      
        1 atagaaggac tgaccttctg tctcagattt ttttgttatt tcacttgaat caattgattg
       61 atcaaaaaaa tcctgatttt catgttctca aatgatttaa atccataaga taatcgtttc
      121 agtgtcttga tatggggaat cttggcctct attttgccat tagaatacgg gtaaatcata
      181 gcattgtaaa taccttcttc atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca
      241 tcaagtgttt caggaagatt ttttaa
//
LOCUS       DACDYJ010000236          262 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDYJ010000236 DACDYJ010000000
VERSION     DACDYJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.denovo.251"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            32..>262
                     /locus_tag="IWM51_002933"
     CDS             32..>262
                     /locus_tag="IWM51_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9972252.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNVQNLNTPLKLQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMQLNEGNGFVK"
BASE COUNT           93 a           36 c           51 g           82 t
ORIGIN      
        1 aatctctttt ttcgttgagg aggagctatt tttgagtaaa aaaggcataa tcgtaaatga
       61 gtgggaaaaa cttaaccgta tttctaacag gcggttaaat gttcaaaatt tgaacacccc
      121 cctaaaatta cagtggtcaa tcgaccgaat taccattgtt ggaaaattga aagaaaatat
      181 ttattatcac acaccaaatg atgtcttgat tttaaatttt gagcaattga tgcaattgaa
      241 tgaggggaat gggtttgtca aa
//
LOCUS       DACDYJ010000237        12090 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.guided.076, whole genome shotgun sequence.
ACCESSION   DACDYJ010000237 DACDYJ010000000
VERSION     DACDYJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.guided.076"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..251)
                     /locus_tag="IWM51_002934"
     CDS             complement(<1..251)
                     /locus_tag="IWM51_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9972253.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGT"
     gene            399..731
                     /locus_tag="IWM51_002935"
     CDS             399..731
                     /locus_tag="IWM51_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9972254.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            843..2051
                     /locus_tag="IWM51_002936"
     CDS             843..2051
                     /locus_tag="IWM51_002936"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP9972255.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            2205..2660
                     /locus_tag="IWM51_002937"
     CDS             2205..2660
                     /locus_tag="IWM51_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972256.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            3022..3297
                     /locus_tag="IWM51_002938"
     CDS             3022..3297
                     /locus_tag="IWM51_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972257.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            3310..5388
                     /locus_tag="IWM51_002939"
     CDS             3310..5388
                     /locus_tag="IWM51_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9972258.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            5548..7434
                     /locus_tag="IWM51_002940"
     CDS             5548..7434
                     /locus_tag="IWM51_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9972259.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            7446..8393
                     /locus_tag="IWM51_002941"
     CDS             7446..8393
                     /locus_tag="IWM51_002941"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9972260.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            8413..8940
                     /locus_tag="IWM51_002942"
     CDS             8413..8940
                     /locus_tag="IWM51_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9972261.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(9003..9656)
                     /locus_tag="IWM51_002943"
     CDS             complement(9003..9656)
                     /locus_tag="IWM51_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9972262.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            9788..10630
                     /locus_tag="IWM51_002944"
     CDS             9788..10630
                     /locus_tag="IWM51_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9972263.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            10788..11684
                     /locus_tag="IWM51_002945"
     CDS             10788..11684
                     /locus_tag="IWM51_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9972264.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            11687..>12090
                     /locus_tag="IWM51_002946"
     CDS             11687..>12090
                     /locus_tag="IWM51_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP9972265.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSL"
BASE COUNT         4188 a         2041 c         2521 g         3340 t
ORIGIN      
        1 gtacctagaa cagtataggc caaggaacgt ttaccgagga tctttcctcc tagtaaaatc
       61 aatgggatgt tgattaataa agtcgtataa gctggatcaa taccaaataa agctcttaag
      121 atcaaggtga tccctgagac tcccccttct gctaaatgat tagcgatgtt caagtaaaca
      181 agaccaaatg cataaatact tgttcctata atgataaaaa gaacttcttt tactgttttt
      241 cgtttgacca tatgaattcc agctttcaat tatgtatttt cacagaattt ccatttagaa
      301 attccttcct aaatgtagca tggaaattcg attttctcaa ttgctcttat ctaggatttg
      361 cgttaaagtt ttggtaagat agttttggag ggaaaaagat ggataaaaag cgttctttat
      421 acagtatgca gcaggaagtg gatgaatata tccagcaatt caaggtgggg tatttctctc
      481 cattagctca gatggctcga ttaactgaag aagtaggtga attggcaaga gaagtcaacc
      541 acacttatgg agaaaaaagc aaaaaggctt ctgaaccagt taactcggta gcagaagaac
      601 ttggtgatgt tttattcgtt acgatgatca tggctaattc attgaatatc gacttaactg
      661 aagtttttga aaaaaacatg gaaaaattca atcaaagaga ccataatcgc tttgagcgta
      721 aggacgagta aacaagatga agtggtcctg gccttgaaga ctctgaaacg gacttgtcaa
      781 acagtcacca tgcaactgag tctttatctc aggacaagaa acacgcagga aaggttggaa
      841 aaatgaaatt aaaacaatta cctctggaat accaaaaggc aattccagtt ctgaaaaaat
      901 tagagaatgc aggatatgaa gcttattttg taggtggaag tgttcgtgat atcttgctga
      961 accagccaat ccatgatgtg gatatcgcaa caagcgcatt tccagaagaa atcaaacagc
     1021 tttttccaaa aacgatagat attgggatcg aacatggaac tgtgttggta ttagaaaatg
     1081 atgaacaata tgagatcacc acatttcgga cggaatcaac ttatcaggac taccgtagac
     1141 cagatcatgt cgaattcgtt cgttcgttgg cagaagattt gaaaagaaga gattttacga
     1201 tcaatgcgtt tgctttgaaa gaagatggag aaatcatcga tttgttcgat ggtctgcaag
     1261 atctagaaga acagatcttg cgagctgtag gcaatcctca tgagcggttc catgaagatg
     1321 ccttacgaat gatgcgcggg ttgcgttttg tcagtcaact agggttcaaa ttagaggaag
     1381 aaaccttcta cgccatcaaa gaaaaccacg agctattagg taaaatatct gtagaacgta
     1441 taaatgtgga attcatcaag atgctcttgg gaaatgaacg ggccgcggga atccatgcat
     1501 ttattgaaac agagtgctac atttgctgtc ccggtttgaa agaagaaggc gaagcacttt
     1561 tacgttttgc tgaattacca tccgtcccat tgaaagaaga gagccaagca tgggcattat
     1621 tgatcagcca actggatcta aaagaaaatg agattcgttc ctttttgaaa tcgtggaagt
     1681 gttctaacca aatgattaaa gacgttcaag cacttgtcca aggactacga caacgagaaa
     1741 ctggagcttt ggaaccattc gaattgtact tcttaggcaa aaaacatgca ctgcaagtag
     1801 aagaattgat gatctatttt gacgaaacac ctcagatgga tgccgtagga gaatcctatg
     1861 atcgtttacc tatcaagtca ttaagcgaat tagcagtaga tggtaaaatc ttgctagaag
     1921 aaactggaaa aaaacctggt aagtgggtta gtgaagcttt gcaattagct gaaaaagccg
     1981 tcgtagaaca aagaataaaa aatgataaaa aacaggtttt ggatttttta tcgaaagaaa
     2041 aattgattta aaaaacacta gactgtatct gctcagcttt gttggctact taaagtgggc
     2101 agatacgtca ttagaagaaa aaacataaga tgtgaccact tacataaatt tgtgctaaac
     2161 tgaggtagta caagaataaa ttacatcata aaggagtctt tttaatgagt aaagaaatga
     2221 cagcgctaaa attttatttt agaaatggag aaacctggac gatcaaccgc cgtcatatcg
     2281 gcgatctttg gatcaaacaa atcacaacaa gttttggacg aatcaatggc agtgaatttg
     2341 ttgaaatcca tccgtgtgct ggatttaaaa tcgaaatctt ccatgaaggg gacgcagttg
     2401 cgacacatga tatcaaccta ggcggactgg aaatgggcat gtttaatcgc gcattgaaat
     2461 acgaagatat cgagcgaatg gaaatccttt accgaaatgg cacacctgat cttgtgtact
     2521 tcccatatct agataaagga acagaaggat tagacaatca atatcaatca acaaaaatca
     2581 gcgaaaaaac aggtaacttg tatatcgtga tcaatccaga tcaacgtgta gaagatgtat
     2641 acggggaatt tttcgaataa atcgattttt acgcaaaaat gaggctgtga aagagatgtg
     2701 tcttattact actgataggc tgtctcttcc acaccgttta ttcagaataa gggactgtga
     2761 caagattttt gtcgcagccc catttttttg ttgtgataaa agaacaagaa aggcaggaat
     2821 gggaaagtaa acatcctttt gtcagaaact tttatctttc ttaagtttta cttgatatct
     2881 gatactattt ggaatagaat gaggcgttga aaaatttatg gaaatttcta actagcaata
     2941 aacgatgaaa caatgaaata tttttcatcc aaaaagaact ttgctataat gatggaagtt
     3001 tgtgtatagg agatctggaa aatgagagag agttttgaac aacaaaaaaa actgttgcat
     3061 gatcgctatg gcgccctttc gatggatgac cgccgtcaaa tactttgtaa gttgcgtaaa
     3121 agaaatatat tgatgtatcg tcagttggaa cgtctgaaac atgatctgct tcgtttggag
     3181 tcaaaacgtg tacagtgtga attagagggg aaccaaacac aggttgaagt agtcgaaacc
     3241 aaaattttaa agaaaaaaga acaattttta aaaatgctta cacaaaataa aaaataaagg
     3301 ggatggacga tggaatttgc tgagttagtt attgtatttg cagtaacgat caccttatcg
     3361 aacatagcta gccgcatttt gccaatgatt ccagctccac tgatccaaat tttccttgga
     3421 gtggtattag gtctgacaga gtggggacaa tcaatcgatt ttgaacctga attgttctta
     3481 gttatgatta ttgcaccatt gttatttaga gaaggagaaa aagcagatat ttcttcgatt
     3541 ctcaaaaatt tcgggacaat tttgttcttg gcatttggag gagtcatttt gacccttgtc
     3601 ggggtaggtg cgacgttatc attcctgttg ccaagtgtcc cacttgctgc ctgctttgct
     3661 tttggagcag cgttaggtcc gacagatgcg gtagcagtca gttctctttc tggacgagtg
     3721 aatatcccta aaaaggcgat gcatattttg gaaggagaag gtcttttgaa tgatgcatct
     3781 ggtgttactg ccttccagtt tgctcttggt gcgctgatca ccggatcttt ttcagcagta
     3841 aacgcgggga tgagtctagt agtttctagt attgggggag ctttgatcgg ctttttgctt
     3901 gtctggttca aacaaaaaat aattcatttg atcgaaaaag catcagcaca agatgtcaca
     3961 gggtatctgc tgatcgaact tttgcttcct tttctagcct atgtattggc agaggcattt
     4021 gatgtatctg gaatcattgc tgcagtggca gcaggtattc tccaagcttc tggttttagg
     4081 aaaatctctg tgtttgatgc agaattgtcg agcttgtccc acagtacatg gacaactatt
     4141 gcttttacac tgaatgcatt agtatttata tttttaggaa tcgaattgac gcaagtgttc
     4201 tcgccagtat ggggagacgg tttatatcca aacggtcttt tattagcgat tatcgtatta
     4261 atctctgtca tgctttttgt gatcagattc atttctatca gtctttttta tgtattcaaa
     4321 gatggcagta aaaagttcaa aaaacagttg aacgagatct tgatcttaac atttggcggt
     4381 gtaaagggaa cagtcagttt ggctacgatt tttattttgc cattttcaat caataatatg
     4441 atgttttacc agcggtcttt gcttttgttt ctgacggcgg gtgtcatctt agtgactttg
     4501 gtcataggga tcattgtctt gcctatgctg acggaaacag aggaagcgaa aagcacggat
     4561 ttgaatgctt tgatgatttt agaagaggtc gttgaagttt tgcgaaaaga aataaaggaa
     4621 attgatcgta atacaaaaga atttttagcc actgaagcag tgatagaaaa ttatcaagaa
     4681 cggatacgtg atctgtactt ggaagattta accgatgatg aaaagcaaga agtacaagaa
     4741 atacaagcac tgattttgtc gatcgaacgg gatggattgg atgaaagtta tcgttcgggc
     4801 aagctttctt ctaatggtta tcgtttttac tcccgttttc tttctcgatt cgaacattct
     4861 atcacaagcc agattttgtc ttttatcggc ttttggttca ttgttgtccg taggttaatg
     4921 cgaatcgttc tccatccgaa aatgttttgg caaagaagat atgctgatcg tcagacattt
     4981 attagtgatg aagatatcca agaaatcaga gagacatatc aaaaaaatac tcagttgatt
     5041 attgaaagtt tagataactt gactgatatt tatgatgaaa cgatggttca attttttatc
     5101 cagcaacgca aacttgaagg aataaaaatg aactcgggga atttgatttc ttcgtggatg
     5161 atccagcaag atagtctgtt tacgaaaaaa atgctaagag gttattatct tgaaagaaaa
     5221 atcattgatg aatacgaagt tgctgaaaaa atcacgacgt tttctgcgaa cagctatcgc
     5281 aggaatatca atttgttaga atcttatacg atgaacaaac catctgataa cttttctttc
     5341 cgatttgcgt ttcgagcaaa gtcaaaaaaa acgagtccaa taaaataaaa aggtaaaaat
     5401 tttcaagttt tataaaaaac aaaacatatc agaacaggat cgaatcggtc ctgttctttt
     5461 tttgagaaca caaaaagaac aaataccaaa aaaattttcc tgaagttcac tatttttatg
     5521 atacaatatc caagataagg agggaacatg ttgaaagaat taaaagtaac tgacctcaaa
     5581 aaaacttatg gtgaaaaaga tttgtttgac cagatttctt ttttggtcca cgaaaaagac
     5641 cgtatcgggt tgattgggac gaacgggacg ggtaaaacaa gtctcttaaa tattattgcc
     5701 ggtatcgata gcagcgacgg tgatcggcaa acagtgtttt atccaactga ttaccgtatt
     5761 ggttatcttt ctcaagcagc tgaattttcc gatgaattaa ctgttctcca agcagtcttt
     5821 caaggaaaca gccccttgat ccaaactgtt cgagcatatg aggaagcatt gatcgaacta
     5881 ggggaaaacg gagaagaccc tgatgtgcaa aaacgttatg ctaaagcaga agaacaaatg
     5941 aataaagaag atgcttggac tacagatacc aacgcaaaga ttattttgca aaaattagga
     6001 atcagtgaat tagataaaaa aatcagtact ttatcaggtg gtcagaaaaa acgagttagt
     6061 ctggcgcaag tgttgattga tgaacccgat ttactattac tagacgaacc aacaaaccat
     6121 ttagattatg aagcgatcga gtggttggaa aactatttga aacaataccg tggcgcacta
     6181 ttgatggtta cccacgatcg ttattttcta gatagagtag caaaccgaat cttcgaactt
     6241 tcatttggaa aattatacga gtataaagga aattatgaag cttacgtctt agaaaaagca
     6301 gaacgtgatc gtgtagaagt agaacaagaa gaaaagcgca agcggcttta taaacaggaa
     6361 ttagcttgga tgagggctgg cgtacaggca cgtggcacca agcagcaagc acggatcaac
     6421 cgtttcgaag atctaaagga gaacttgtac caagtaaacc aagaagatga tttagagctg
     6481 aacctagcga cacaacgttt agggaaaaaa gtactggaaa tcaaagatgg cagttaccgt
     6541 ataaatgacc aaacactgct agaacattta gatttattga ttcaatctcg ggaaagacta
     6601 ggtattactg gtaaaaatgg tgcgggaaaa tcgacattgc ttaatattct agctggcaga
     6661 attcccctgg atagcggtac aatgtctatc ggtgaaacgg ttcatcttgc gtattataca
     6721 caagaaaatg aagaaatggc accagataaa cggatgattg cttacttaca agaagctgct
     6781 gaagaagcaa aaacagcaga tggcagtcaa atcggagtag cagaattact tgaacgattt
     6841 ttatttccaa gatttatgca tgggacactg ataggcaaac tttcaggcgg agaaaaacgc
     6901 cggttatttt tgttgaaact gctgattcag caacctaatg ttttattatt ggatgaaccg
     6961 accaatgatt tggacatagc aacattgacg attttggaag attattttag aagttttcca
     7021 ggtgctgtga tcactgtttc ccatgaccgt tatttcttgg acaaggtagc agataagtta
     7081 ttagtattcc aaggaaacgg aaaacaagaa ctttattacg gaaacatgtc cagttattta
     7141 ctgaagcaaa aagagaccca gcagcctgct gaaaaagcga aaccaaaaat acagagcaag
     7201 gaacctgctg aaaagaagaa attatcttac atggaacaaa aagaatggga aacgattgaa
     7261 gatgagattg cagaacttga agaaaaaatc tcattgctcc aagaagagat gaatcatcaa
     7321 ggagataatt tcactcgatt acaagaattg caaaacgacg tatctgaaac agaagcacaa
     7381 cttgaagaaa aaatggcacg gtgggaatac ttgagtgaat gggtagaaga ttaggaggaa
     7441 aaaggatgga acaagcgtat cttgacttag gaagaaaatt gttggaagaa gggcatcgta
     7501 aagaagacag aacaggcaca ggcacattaa gcttattcgg ataccaaatg cggtttgatc
     7561 tgcaaaaagg gtttcctttg ttaacaacga aacgagtacc ttttggattg atcaaaagcg
     7621 aattgctttg gtttttaaaa ggagatacga atatccgcta tctattacaa aataaaaacc
     7681 atatttggga tgaatgggca tttgaacgct acatcaaaag tccagactat aatggaccag
     7741 atatgactga ttttggccat agatgtttga cagatcctga attcaatgaa gaatataaaa
     7801 agcagagcaa gcttttttgc gagaagatcc taaccgatga ttcctttgct gaaacatacg
     7861 gcgatcttgg acacatctac ggctatcaat ggcgtcactg ggaaacaaaa gacggtggct
     7921 ttatcgatca aatcaaacaa gtgatcgaag caatcaaaaa gactcctgat tctagaagat
     7981 tgatcgtttc tgcatggaat ccggaagatg ttcctagcat ggctttgcct ccttgtcata
     8041 cgatgtttca attttatgtt caagagggac ggctaagctg ccaattgtat caacgcagtg
     8101 gggatgtgtt tctaggcgtt ccgttcaaca ttgcaagtta tgcgttgctg actcacctaa
     8161 ttgcacatga aacaggttta gcggttggcg aatttgttca cacattagga gatgctcatc
     8221 tttatatcaa tcatatcgaa caaatgaaag aacaattgag ccgagaaata cgcgcatttc
     8281 cgactttagt tttgaatcag gaaaagcagt cagtatttga ttttgagatg gaagatatcc
     8341 aattagaagg atatgaccca catccggcga tcaaagctcc tatcgctgtt tagaatcaga
     8401 aaggatgaga aaatgttagc tgcaatttgg gcacaggatg aaaacggatt gattggaaaa
     8461 gaagaccgtc ttccttggcg tttgccaaat gatctgaaat tcttcaaaca aatgacagaa
     8521 gcaaatacac tagtgatggg acgaaaaaca ttcgaaggga tgggtagccg tccgttgccg
     8581 aatcgtcaga cgattgtttt gacgagagat tcttcctata aagcagaagg cgtgcatgtc
     8641 atgcatagtg tggaagacgt gcttgctctg gaaaaagaaa cagacggtat ctttttcatc
     8701 gctggagggt cagctgttta tcaagaattt ctgccatttt gtacgatatt ataccgtaca
     8761 gtcatccatc atgtattcga tggagatgct tattttccac ctgttgactg gtctgattgg
     8821 tcactgatca atactagtca aggagaaatg gatgaaaaaa atcgttatcc gcatcaattt
     8881 gaaacgtatc agcgtaatga gaatcctgta aaaagcggga caggcgaatt gacgatataa
     8941 aaaagctatg gatagactag ctgttattta gagctgtccc atccatagct ttattttttt
     9001 tattaagtaa agaataaaac agagaagtac ataaaacctg cacctaacat aacaaacaaa
     9061 tgccaaacaa catgcatata gcggatagaa ttcaagctat aaaagaatgc gcctatcgtg
     9121 taagaaagcc cgcctgctac catcaaaata acaccagtca ttccaagaga gtgataaaga
     9181 ggaacagcag ccacgacaca gagccacccc ataaaaatat agatgatcgt agatactttt
     9241 gatactctct cccgtttatg caacgtcaac gctttatata taatgccaag aatcgcaaac
     9301 aaccagatta atacaaataa agtccaacct agccatcctc ggatactcag caaacaaaaa
     9361 ggagtataac ttcctgcaat caaaagaaaa atcgaactat gatcgaatac ttggaacacc
     9421 ttttttgctt ttgtaaaaat caatgaatga aacaaagtag acgttaaaaa taaaagaatc
     9481 atcatcgatc catagatagc ataagagaca atatggaggg aggaatgcaa gtgggcacct
     9541 tttactaaaa gaaccactag tcccgcgata ctcaatccag tcccaattcc atgggtgact
     9601 gcattaaaca cttcatttaa tatttcatac gttcgattaa atttcgtttt ttccatgaaa
     9661 acggctcctt tactaggtgt aattaatgaa aaatcattat ttgtctgtta tactaaaaat
     9721 agaaattgtc ttacatgtag tttaacatga ataaggcatc ttgttaaaga aagagagaga
     9781 gaaaataatg acaaatgtaa aaatcgtaac agattcttca tgtacgatgg aaaaaaatgt
     9841 acgcgatgct ttaggcattc gtgtcatgcc gctatccatc atgatcgacg gaacagtata
     9901 tcccgatgat gaccaactag acggcgaaag atttatggaa atgatggcga aagcaaaaaa
     9961 tctgcccaaa acaagccaac caccaattgg agaattcatc tcgatgtatg atgagctagg
    10021 agaagacggc agtgaagtga tttcgatcca tatgacaaaa ggcttgagtg gaacagtaga
    10081 agcagcaaga caagccagta atctaacaaa aacgaaagta acggttatcg acagtgatac
    10141 cactgaccaa ggattatctt ttcaggtaat tcgagcagct gaattagcaa aagaaggtaa
    10201 aacagtagag aaaatccttg aagaagtcaa aaaagtacaa gacaatacaa aattatacat
    10261 cggaatatcc acactagata acttggtaaa aggtggaaga atcagccgaa caactggttt
    10321 gctgtctagt gttttgaaca tacgcgtggt gatgaatttc gcccattctg aattaatccc
    10381 tgtaactaag gggcgaggaa agaaaacgtt cgataaatgg tttaataatt tgaaaaatga
    10441 actgaaagaa ttgccaaatg tgcgacaaat cggtatttca catgcggatg caaaagaatt
    10501 ggcgcaagaa ttcaaagagg gtttgcaaga attattcccg gacatgcata tccctgtact
    10561 gcatacgaat ccggttatcg ctacgcatac tggtaaagac gcgtttgcaa tcatgtatta
    10621 tgtagattag tcgaaaagag gataagccct cagcctgaaa ggcttgcagc ttattctctt
    10681 tttctttata aaattaggaa aataattgtt ttcttttttc ataaagacta cctttttgat
    10741 aaaatttttg ataacattag aaccaatcga atacaagagg tgaaaaaatg aaattaacga
    10801 aacgacattt gatcctcgtt ttgttcccat tattaattgc gatcatcatc tatgcaggtt
    10861 tagctgcttc tataccgaaa gctggcccac taatgaaacc agtaactact gtgagagaag
    10921 atccaaataa aaaagaaaaa cttcactaca tagctatcgg agattcgctg actgaaggag
    10981 taggagatca aaccaagcaa ggaggattcg ttccccttgt cgcaaatgat ctaaaagacc
    11041 gttataattt gaccgcggtt gaaacagaaa actatggtgt aaatggtgaa cgaagtgacc
    11101 agatcttaaa gcgagtaaaa acaaaagaag aaattcgaaa caatatcaaa acagctgatt
    11161 ttatcacact gactgttggc ggtaatgatt tgatgaaagt tatccaaaat aatttattcg
    11221 ggttaacggt caattccttt aaaaagccac tgaaaaacta tcaggaaaat gtcacagaac
    11281 tattggaaga atttcgatcc ctcaataaaa ccgcacctat ttacatttta ggcatctaca
    11341 atccatttta tttgaacttt cctgagatca ctgacatgca gacaattgtt aacaattgga
    11401 acaaaggaac agaaacggtg acaaaaaaaa tgccgaactg ctactttatc ccaatcaatg
    11461 atttgcttta tcaaggattg aatcaggaag taggtgtcac agaagaagga acagatcaaa
    11521 caactagcac gacagaaaat ctaaatggca tcaaaaataa tgtgctctat gaagaagatc
    11581 atttccatcc aaataattta ggttatcaat tgatggcaaa tgcagtgaga gatcagctga
    11641 tcgagacaca aaaccaatgg ttagagaagg aggatgatca ataaacatga aaacaagatc
    11701 tgacgtatct tcaaacaaaa caaagaaaac aaattactgg aaattcgcct tccttatcct
    11761 tcttggggta gtacttggtt cgaccgtatt tttaggcagc cgtatttttg ctaatcggga
    11821 accagaactc ccagaaatac cagctcttac ggaaagacaa ggagatcctg tcttaacgat
    11881 caattcaaat aaagaaaaag tcaatcaaat catcagcttt ttccttagtg agtatcaaaa
    11941 agatagtgat attgaatata aattttattt ggaaaatgaa gcactactca atggtacttt
    12001 cgaagtacta ggttttccta tcgatttcta tttatatttt gatccgtatg tgatggaaaa
    12061 cggaaatgtc caactaaaag ctagaagtct
//
LOCUS       DACDYJ010000238         7203 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.guided.104, whole genome shotgun sequence.
ACCESSION   DACDYJ010000238 DACDYJ010000000
VERSION     DACDYJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.guided.104"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..866
                     /locus_tag="IWM51_002947"
     CDS             <1..866
                     /locus_tag="IWM51_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9972266.1"
                     /translation="VIESAQALGLDLLDKPTDQTIRLASSYALGELIKDALLQEVEQI
                     IVTLGGSGCSDGGLGLLQSLGASLEGYTEGNPLLSTKKLHFDRRNVLVSKVKLIIAAD
                     VTNPYTGQQGAAWQFGRQKGGIDTTLAFLDKQAGKIAQQIKDFWEVDLDDIPGSGAAG
                     GIGGALFLLGAKMVSGFDLVSQITGLEKKIEQADVIITGEGRIDQQTIHGKVPHGVAV
                     LAKKYRKPIIAICGSRDQELEGLSDFLPIVLSIHLGPIGLEEAMEHRQTLEKTKILGQ
                     TLSRFFTILPK"
     gene            1124..2626
                     /gene="lsa"
                     /locus_tag="IWM51_002948"
     CDS             1124..2626
                     /gene="lsa"
                     /locus_tag="IWM51_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9972267.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKSQPKHLSISYVRQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2693..3220
                     /locus_tag="IWM51_002949"
     CDS             2693..3220
                     /locus_tag="IWM51_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9972268.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            3431..4492
                     /locus_tag="IWM51_002950"
     CDS             3431..4492
                     /locus_tag="IWM51_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9972269.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            4495..5391
                     /gene="murQ"
                     /locus_tag="IWM51_002951"
     CDS             4495..5391
                     /gene="murQ"
                     /locus_tag="IWM51_002951"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9972270.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            5405..>7203
                     /locus_tag="IWM51_002952"
     CDS             5405..>7203
                     /locus_tag="IWM51_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9972271.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSAATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVT"
BASE COUNT         2392 a         1270 c         1638 g         1903 t
ORIGIN      
        1 cagtcatcga atctgcacaa gcattggggt tagatctact tgataaacca acggatcaaa
       61 ctatccgact tgcttccagt tatgccttag gagaacttat caaagatgca ctgcttcaag
      121 aagtagaaca aattattgtc actttaggag gaagcgggtg tagcgatggt ggtctaggat
      181 tactccaatc cttgggtgct tctttagaag ggtacacaga aggaaatcct ttattatcta
      241 ctaaaaaact tcattttgat cgaagaaacg ttcttgtttc taaagtaaaa ttgattatcg
      301 ctgcagatgt taccaatcca tatacaggtc aacaaggagc tgcttggcaa tttggaagac
      361 aaaaaggagg aatagatacg acgctagctt tcttagataa acaagcagga aagattgctc
      421 agcaaataaa agatttctgg gaggtggatt tagatgatat tccaggaagt ggagcagcag
      481 gcggtattgg tggagccctt tttttgttgg gagcgaagat ggtttctggt ttcgatttag
      541 tcagccaaat cactggattg gaaaaaaaga tcgaacaagc agatgtcatc attacaggag
      601 aagggcggat cgatcagcaa acgatccacg gaaaagttcc acacggtgta gcagtgcttg
      661 caaaaaaata tcgaaaacca ataatcgcta tttgcggaag tagagaccaa gagctagaag
      721 gactctctga ttttctccca attgtcttaa gtattcattt gggtccaata ggtttagaag
      781 aagcaatgga acacaggcaa acattagaaa aaacaaaaat cttaggacag acacttagca
      841 gatttttcac catcctcccg aagtagttga caaaatcatt ttctttatgc tatcgtaatt
      901 tcaaatatag gacaaagtga gggatccgtg cattgaaaaa ttaagtcgat tgatgaaagc
      961 tgcattcaga ctgtaccttg tggagacaag gaagcagtgt gcagattttc gggattggct
     1021 tggttttttc gatgtatggg tctttttgtg tgtatccaac ccttgcatcc tctttagcca
     1081 gttctgaaaa taggactggt ctttttttac gaggtgatgg aaaatgtcta aaatcgaaat
     1141 aaaaaatctg acattcggct acgacagcca aggcacatta ttatttgaac aagcaaatct
     1201 aaattttgac acacaatgga aactaggact tatcggacga aacggtcgag gaaagacaac
     1261 tttactgaat attctacaaa acaaactacc ttatcaaggg caagtaatcc atcagcaaga
     1321 atttgcctat ttcccgcaac agacaaaaga taaagaacgt ttaacctatt acgtgttaaa
     1381 tgatattacg gattttgaga tatgggaaat cgaaagagag ctccaattga tgcaaacaga
     1441 tcctgaaatc ttatggagag aattcagcac actatcgggg ggagagaaga caaaagtcct
     1501 actggcactt ttatttgtgg atgacactca tttcccgtta atcgatgaac caacgaatca
     1561 tttggatatc tctggtagaa aacaagtagc ggcttatttg aaaaagaaaa aacaaggctt
     1621 catcgtggtc agccatgacc ggggatttat cgatgaagta gtggaccatg ttttagcaat
     1681 cgaaaaaagt caactggaac tttatcaagg gaatttctct atctatgaag aacagaaaaa
     1741 acttcgtgat gaatttgaaa tggctcaaaa tgaaaaattg aaaaaagaag tcagtaggct
     1801 aaagaaaaca gcagctgaaa aagccgaatg gtctcgttcc cgagaaggag ataaaacaaa
     1861 gaaacaagtc ggattcatcg atactgaatc tagacgagtg aataaaggag cagtgggtgc
     1921 tgatgctgca cggacgatga aacgatccaa agcaatcgtg aatcggatgg agacccagat
     1981 cagcgagaaa gaaaaactat taaaagatat cgaatatatc gattctttga cgatgaatag
     2041 ccaagcgtct caccataagc gacttttaag cgtagaagat cttcaattag ggtatgaaaa
     2101 tctgttattc gagccaattc attttacaat cgagcctcat cagcgggtgg cgatttcagg
     2161 tcctaacggt gcaggaaagt catccattat ccattatctt ctgggggcat tcaacggcaa
     2221 ggttatagga gaaaaatccc agccaaaaca tctgagcatt agttatgtac gccaaaatta
     2281 tgaagacaat cgaggaacgt tggcggaatt tgcagagaaa aaccaagtag actaccaagc
     2341 atttttgaac aacctccgaa agcttgggat ggaaagagat gtttttcata acaagatcga
     2401 gcagatgagt atgggccaac ggaaaaaagt ggaattggct aaatctttat cacagccagc
     2461 tgaactatat acatgggatg aaccattgaa ttatttggat gtcttcaatc aagaacaatt
     2521 agaacaactg atcttgaacg tgaaacctgc catgttacta gtggaacatg atcaaacctt
     2581 cctggataaa gtatctactg agattatttc tcttgagaga atctaaccct tgtaatctca
     2641 agaacaacaa ggtatggtta aggggaactt tgccaacgaa ggagagaatc acatgcttta
     2701 tgtaacgaga cacggagaaa caacttggaa cgcacaagga ttagtttgcg gacgggcaga
     2761 tgtgccgctg actgaaaaag gacagatgca ggcacagaaa ctagcagaaa aggtagtaga
     2821 tttgcctgtt ccaatcacga aaatcatcca ttcccctctt caacgagcaa gagatacagc
     2881 acaagcagtt gctgatcgtc tcagcttgcc gctgaccgtt gatgaacgat tggtagaaat
     2941 ggattttggg gattatgatg gactgccaag caaagatgag aactttcaaa aagcacgttt
     3001 agcgttcgct gtacgattcc caaatgggga gtcagtctta gatgtgtatg cacggatcgt
     3061 tcctttattg aaagaatgca tcgaagacga agaaaatgtc tatctgttag tttgtcataa
     3121 tgccttgata agagtgatca atgcttattt tcatccgatg cctaatgagg ggttctttac
     3181 attcatggta gataatacag aattggtttc ttatgagtaa ttatgttttt gtgttattta
     3241 aataaaatat ttcaaggcac tgcttttgct tagcagtgcc ttttttaggt tatgacaatt
     3301 cttttgataa tataaactca acctttagaa atgaaattta attattttaa taaaagtatt
     3361 gaaattttgt ttctttagat atatactcta gtcatagaaa tgaagacgga tacagaaaaa
     3421 gagggataat atgtttggct tttcaatatt tatgaacgat tctttgtcag aagagaagaa
     3481 acagtatatc cgccaaatgg catccaatgg ctttgtcggt attttcactt ccatgcatat
     3541 accagaagat gatgtttcgg catatcaaaa gcgattgatc gaactaggaa gcgaagcgaa
     3601 aaagaataaa ctagaattga tggtcgatat ttcaggagag gcattggaac gagcgggttt
     3661 ttcattagaa gatctaacgc ctttgaaagc aattggtgta acaggtttaa gaatggatta
     3721 tcatatttcc aatcagcaga ttgccgactg gtcacatcaa ttgaaaatca gtttgaatgc
     3781 tagcacgatc acgcctaaag atatcgatga attgaaagaa gcagaagctg atttctcaca
     3841 gattgaagct tggcataatt attatccacg tcccgaaaca gggctggaca aagaatggta
     3901 tcagaaaaaa aatcaatggc tcaaaattca aggtctcctt gtccaaggtt ttgtaccagg
     3961 agatacagag ttgagaggcc cactatatca aggattgcca actttggaag aacatcgagg
     4021 gatccatcct cttgcaggcg ctctagatct attagcaagt aacacggata ttgtttacat
     4081 cggagatgca ggtctgtcag aaaatgttca ggagcaattt gcttctttcc aaaaagaaca
     4141 gacagtcttg ctccatacgg aacctgtaga tgaagaattt tacgaatata tattagggaa
     4201 gcatacgaat cggcaagacg atgcaagaga tgtcatacga agcgcagatg cacgttttcg
     4261 agagattccg cctatcccag caagaaatac agctacgcgt atgaaagggt ctattacttt
     4321 agataacgaa aagtatttgc gctatatggg tgagatccaa ttaaccaaat atgatttgcc
     4381 agcggatgaa aaagtgaatg tagtagctaa agttatcaaa gaagagctac cattgatcaa
     4441 ccaaatcaaa gctggtatga actatcaatt tataagaaaa gaaggaagat aaaaatgagc
     4501 aaaatcaatt tagagaacct aacaacggaa cgcagaaacc aagaaacttt tggattagat
     4561 gagatgagta tttctcaagc cgttcaacta atgaacaaag aagatcagaa agtggccgct
     4621 gcggttgaaa aagaactgac aagtatcgaa ccagtgattg ctaacacgat tgagtccttt
     4681 aaaaaaggtg gacgtctgat ttatatggga gccggaacaa gtggaagact aggtgtgtta
     4741 gatgctgctg aatgcgtacc gacattcggt gtagaaccgg aaatggtcat aggtctaatt
     4801 gctggtggag aagaagcaat gactgtagct gtcgaagggg ccgaagattc ttatgagcta
     4861 gggcaagaag atctaatcaa gctgaatttg acaaaaaatg atatagttat tggtatcgca
     4921 gctagcggtc gtacaccata tgtgatcggt ggattagatt acgcaaaatc gatcggtgct
     4981 gtcacaggaa cgatttcttg caataaacaa gccgaaatca gtaaacacgc ggatttccca
     5041 atcgaagtcg atgcaggtcc agaatttttg acaggctcta ctcgattgaa gtcaggaaca
     5101 gcacaaaaac ttatcttgaa tatgatttct actatcagta tgattggtat aggtaaagta
     5161 ttcaataact tgatggtcga tgtcaaacca acaaatgaaa aactaatcga acgctcaaaa
     5221 cgaatcatca tgcaagcgac agaagctgat tacgaaacag ctgaaaagta ctttgaagaa
     5281 gcagatcaaa atgttaagct ggctatcgtc atgattttga caaatagtga taaagaggaa
     5341 gctcaagcaa aattagctga agcaaacgga tttgtaaaag ggaccttatg agggggaaaa
     5401 gaccatggca aaaaacgaac tgactttgga agaactggca aaacaaattt ataaaggggc
     5461 aggcggcatg ggcaatgtag aaagcattgt ccactgcatg actcgtgtgc gtatgaagat
     5521 catcaatgat gatcttgtaa atgtctctga attaaaagcg attccaggag tgcttggtgt
     5581 agtagaagat gaacagcttc aagtcatcat cggacctggg aaagtgaaca aagtagcaca
     5641 gcaaatggtt gatttggcag gcgtgaaatt aggagaaaaa ctttcttctg taagtgcagc
     5701 tacagcaaat tcatcagcat ctggaaaaga ccgtgtaaac gaacgggcac aagaaatgaa
     5761 agcggcacaa aaatcgaaac aaaaaccatc taaaatcaaa tcggtattaa aagacatttc
     5821 taatatcttt gtgccaatga ttcctgcatt tgtcggtact gggatcgtgg cagggattgc
     5881 cgcagtttta gcaaacttag tcacagccgg aacacttgat gcagcaacat ggcaacaata
     5941 tatcgatgtg atgaacatct tgaaaaacgg gatgtttagc tacctagtta tctatacagg
     6001 tatcaatgct gcacaagttt ttggtgcaac accaactctt ggtggagtta tcggggccgt
     6061 tgtgatgctg acagggatga atccggaagc accactgaaa aacttattta ctggtgaagc
     6121 tcttgcagct ggacaaggtg ggatcctagg tgtcatcttc gctgtttggc tattgtcaat
     6181 cgttgagaaa aaactgcata aagtagtacc tgatgcggta gatatcatcg ttacaccaac
     6241 tctttcattg attgccatcg gtctgattga aattttcttc atcatgcctt tagcaggttt
     6301 catttctgat ggcatggtcg gtgggatcaa ctgggtactt ggcgttggtg gagcatttgc
     6361 cggcttcgtt ctaggcacat tattcttgcc gatggttatg ttcggactgc atcaaatttt
     6421 gactccaatc catgtgcaaa tgattgatga aactggtcgt acgctattgc ttccaattct
     6481 tgcgatggct ggtgcaggac aagtgggtgc agcattggca ttatggattc gctgcaaaaa
     6541 agataaagaa ttaacagaaa tgatcaaagg agctttacca gtcgggatcc taggaatcgg
     6601 cgaaccattg atttacggag taacattacc tctaggacgc ccatttatta ctgcttgtat
     6661 cggtggaggt atcggcgggg cagtgatcgg aatgatcgga aatgtcgggg ctatcgctat
     6721 tggaccttca ggagccgctt tgatcccatt gatctctgac ggtaaatggt taggctacat
     6781 ccttggatta ttggcagctt atgctggtgg cttcgttgct acattcttct tcggtattcc
     6841 taaagaccgt ttagaaaaag aagaattagc gactgaaaca gccacagtaa atgaagtgcc
     6901 tgaaaaagta gtacctcaaa cttcagctga aagtgtgact atcctctcat cagttgctga
     6961 cggaacagtt gtttctttag ataaagcaag tgatccagtt tttgcacaaa agatgatggg
     7021 tgaaggctac tttgtagaac cagaaaacgg acatatctat tctccagtta ctggggtagt
     7081 cagctctgtc ttcccaacga aacacgctat tgggatcaca acgccaaatg gtttagaaat
     7141 cttgcttcat atgggaatca acacagttga cttaggcggt acgccatttg atgtgaaagt
     7201 aac
//
LOCUS       DACDYJ010000239         4511 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.guided.135, whole genome shotgun sequence.
ACCESSION   DACDYJ010000239 DACDYJ010000000
VERSION     DACDYJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.guided.135"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            267..731
                     /locus_tag="IWM51_002953"
     CDS             267..731
                     /locus_tag="IWM51_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9972272.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            715..1344
                     /locus_tag="IWM51_002954"
     CDS             715..1344
                     /locus_tag="IWM51_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9972273.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1698..3176
                     /gene="msr"
                     /locus_tag="IWM51_002955"
     CDS             1698..3176
                     /gene="msr"
                     /locus_tag="IWM51_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9972274.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3223..3669
                     /locus_tag="IWM51_002956"
     CDS             3223..3669
                     /locus_tag="IWM51_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9972275.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3868..4383)
                     /locus_tag="IWM51_002957"
     CDS             complement(3868..4383)
                     /locus_tag="IWM51_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9972276.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
BASE COUNT         1464 a          752 c          922 g         1373 t
ORIGIN      
        1 cgatttcgga aatttttggc ggtgtaaaat tttctactgc agaagagcta tcttctatag
       61 tttgataaga atcaataatc ttggttttag cgttgtgctt tctttatcat tttttccgtt
      121 cattctacaa aaaatttctt ttctctatac ttttctattg agagcgtttt ttttattaca
      181 cagctgattc tttgactttt ttcttttttt attctattat tgacaagtca atcgttgacg
      241 agtcaattat tagaaaggag gaaattatgt ctttagaaga gattagtcac cttttatatc
      301 aaatcaaatt agccgatcaa aaaatcgtcc agatctttga agaaaatatc ggtataagtc
      361 ttactagata cgagatcatg atgagactga aagaagaaaa tccattgcta caaagcagtt
      421 tgcaagaaaa gttgcagatt gatcaaggag cagttactcg tcatctgaag gtattggaag
      481 caaatggcta tgtttcgcgt cagagaaatc ctcagaacaa ccgggaagtg tatgtagcgc
      541 taacagaaaa agcccagaag aaaatcgagc attgtgaaat ggaccataaa gatacaggtt
      601 ccgtgctatc cccggctttc agcgatgtag aaatgaaaca atttctaaca cttttagata
      661 aattgaatac ccaactttgc aatataacaa cagaaacgaa agaggttaat aataatggaa
      721 caaaaaaatg atttttcaac aattatgact ggtagaagat ccgttcgcgt atatgatgaa
      781 acatacaaaa tcccacacga agaaatgctg gaaatgatca aagaagcgac gactgcacct
      841 tcttctgtca atatgcaacc atggcgcttt gtggtcgctg aaagcgagga agcaaaagaa
      901 atcttgcgtc ctttgatccg tttcaatacg agacaaaatg atacttcttc tgcaatggtc
      961 atgatttttg gcgatatgca atgttacgaa tatggcgaag aaatctataa ccaagcttac
     1021 gaatcaggga agatgccaaa agaagtcaga gatcaacaat tagctgcgat cattccttat
     1081 tacaagagtt tttcaagaga agaaatgaac gatgtagtca aagtcgattc tagcttagcg
     1141 gctatgcaat tcatgttagt tgcgcgtgat catggctatg aaacaaatcc aattggtgga
     1201 ttcgaagcag atcagttggc agaggcattt ggtttggata aagaccgtta tgttccagtt
     1261 attattttat caataggaaa agcggtagaa gaaggttacg agtctgttcg tttagcacca
     1321 gaaaagatca ctacgttcga ataaaaggtt ttcagttact attcagtagc tattgctttt
     1381 tttgaatcgt gtgtggtaac ctaataaaaa aggaggtcgg aaaaatgact gcatcgatga
     1441 aattacgttt cgaacttttg aataacaact aaataaattc aaaagacatg tataaacaac
     1501 aatttcatgg atggagtatg tccatttttt cgaacgacag tagaataggt gaaagctatg
     1561 acagatttgg tgatttagtc atagttattt cgttcagagt aggaaaagat gtgatgcagt
     1621 ttttttgcaa acagattttt ctgctctttt ttagtatcga taattgttgt tttacatgaa
     1681 ataggagtgt aaaacatatg gaaaatttag cagtaaatat aacaaatcta caagtcagct
     1741 ttggcaacca gctagaacta tctattgatt ctcttcgtgt ctatcagcaa gatcgtatag
     1801 ggatcatagg agaaaatggt gtcggtaaat cgactttgct caaactaata gccggtgaac
     1861 tttttcccga tcatgggaaa atccaaacag agatcacctt caactacctg cctcaattaa
     1921 cctatctcgc tgaagcaaag gacctaaatt tggaattagc cagtcatttc cagttaagac
     1981 tggaagaaac ttcagagcgg aaatggagcg gaggggaaga acgaaagatc gagttgatac
     2041 gtcttctttc ttcttatgaa caagggatgc ttctagacga gccaacaacc catctagata
     2101 gaaaaagtat tgatcgactg attgaagagc ttcgttatta ttatggtacg ctggtttttg
     2161 ttagtcatga tcgctatttt ctagatgagc tagcatcgaa aatctgggaa gtaaaagacg
     2221 gagaaatccg agagttttcg gggaattata gtgcctatct cactcaaaag gaattggaga
     2281 aaaagactca gctacgagaa gcagagtcga tcatgaaaga gaaaaaacga ttggaaaaat
     2341 cgatccaaga aaagaaaaaa caagcggaaa agttggaaaa agtgtccagt aaaaagaaaa
     2401 agcaacaaat cagaccggat cggttgtctt cctctaaaca aaaagacagt gtacaaaaag
     2461 ccatccaaaa gaatgcgaaa acattagaga gaagactcca aaaaatagga gaaacaacca
     2521 agccgcagca gatgaaacaa atccgttttc cagtaccaaa atctcttgaa ctccacagcc
     2581 gctatcccat catgggacaa aatgtccaat tggaaagaag tgggagaaca ttactggtaa
     2641 atggtgattt tcagttttct ttaggtaaaa aaatcgcgat tgtcggtgaa aatggttcag
     2701 gtaagacaac cttattagaa catatccgca aacaaggaga aggaatcctt ctctctccga
     2761 aagtaagctt tcaagtatat cagcaaaagg gttatcaaat gacatctgaa gaatccatca
     2821 ttcgttttgt catgagacaa acagagtttt cagaatcgct tgtccgtagt ttgctgaatc
     2881 acttagggtt tgctcaggaa actttgacga aaccgttatg tacattaagt gggggagaag
     2941 cgacccgtct gacgattgct ttgcttttta ctaagccaag taatgtgttg ctgttagatg
     3001 aaccgactaa ttttattgat atggcaacga tcgaagcttt agagaagcta atgcaaatat
     3061 atccgggaac gattttgttt acttctcatg attcttactt tgtcgagcgt acggctgatg
     3121 aagtttatga aataaaaggg cagaaaataa aaaaagtact tacgagaaat ttttaaatgt
     3181 gctacgatga ttttcgtgac aaatttaaag gagtggtaca gattgcctac aaataaaaaa
     3241 gaaggaataa tttttacaac aatgatgtgt tttttgatgg tcttggggat gagcgcgtat
     3301 aatttatggc tgcatcaaga tcttcattta tcaagtttgt tgattggtct agtgcctgga
     3361 ttcgtggtgg ctttcatttt ggatgtcttc atcatcggtg tgctggcgaa aaaaattgcc
     3421 tttaaactac cagtaaatcc agaaagcaag gtccaaatga tcttaactat ttcatgcttg
     3481 atggttttag gaatggtgac atgtatgtct ttattcggtg tgatcatgga acaaggaata
     3541 cctgatcaat tattctctgc ttatatgaat gcttggaaaa tgaatgtgat tgccgcttta
     3601 ccgttgcagt tgatcatcgt tggcccgctt tcaagaagtg tgctaaaagc aattcaaagt
     3661 aatgaatgat ggaacactgt tatttgaaaa aaggccttgg catgcttttt gtcatagtct
     3721 cttgaatgca gttctagaaa aatagtgatc cgaacgatag gttttggcct gtcgttcgga
     3781 tcgctttttc ttttccaact gtattgctct tttattgaag ataatttgct cttcaataga
     3841 tatttctaga aaggttctgt ttatccctta ctcaaaaaac tgctgcattt gttctcttac
     3901 ttgctgacac cattggagtt cctgctgaca aagcataatt ctgcgctgga tcacaacata
     3961 acgtccaaat tcttgtttct tgtctagcga gaggtcattc aatttttttt gatttttttg
     4021 catgattttg ttcagctgtt gttctctagt gaatagcagt ccagtagctg ttgttttatc
     4081 gagcgtcgaa aaagcataga tttttgctag aaattcatct ttttgcgtag gagcgggagt
     4141 ttcctctttg atccattcct ctacaagagc acgtccttca tcagtcaagt ggtagatctt
     4201 tttttgagag tgctcgtctt tgtccaaaac ggtcacataa ccctgctggt ctaacttggc
     4261 aagcgttgta tagatctgac tgtgatgggc ttcccaaaaa attgtgatca gctgcttcaa
     4321 ttcataccca gaacgtggtt tggataaaag cacgcataat aatatatagg ctaaagtatt
     4381 cataaaaatc ctacctctcg tcttattttc tttattatat cacagtaaag tttccacgtt
     4441 ttacatgtgc atatttacat gtaaaataaa tcatgtatga tgctaataga gagaaaggga
     4501 tggaaatatt g
//
LOCUS       DACDYJ010000240         2977 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.guided.146, whole genome shotgun sequence.
ACCESSION   DACDYJ010000240 DACDYJ010000000
VERSION     DACDYJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.guided.146"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..900
                     /locus_tag="IWM51_002958"
     CDS             <1..900
                     /locus_tag="IWM51_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9972277.1"
                     /translation="KNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFSFAIFK
                     HFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIENLKAYL
                     TEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGENTKTVH
                     SAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINEFLTTF
                     FKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEYIDQQT
                     KATQVSQFDLVLEKNGSNWKIIE"
     gene            1145..1261
                     /locus_tag="IWM51_002959"
     CDS             1145..1261
                     /locus_tag="IWM51_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP9972278.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            1277..2938
                     /gene="tet"
                     /locus_tag="IWM51_002960"
                     /pseudo
     CDS             1277..2938
                     /gene="tet"
                     /locus_tag="IWM51_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
BASE COUNT         1026 a          488 c          601 g          862 t
ORIGIN      
        1 aaaaatcaga atacagaaaa acagacacca aaggaaaaga aacctcgtgt ctataaggtc
       61 aatcctcgta aaaaggttgt gattgtccta tgggtacttt taggacttag tttcagcttt
      121 gcgatattca agcactttac agccattgat acccatacca ttcatgaaac caccatcata
      181 gaaaaggaat atgtcgatac tcatcatgtg gaaaattttg tagagaactt tgcgaaagtc
      241 tactattcat gggaactaaa cgacaagtcc attgataatc gcatagaaaa cctgaaagca
      301 tatctgacag aggaacttca agctctgaat gtcgatacag ttcgtaaaga tattcctgta
      361 tcgtcttctg taaaaggatt tcagatatgg acggtagaag cagatggcga caatcaattt
      421 gatgtaacct acagtgtaga ccaactcatt acagaggggg aaaatacaaa gaccgtccac
      481 tctgcttata tagtgagtgt ctatgtagac agtactggaa atatggtcct cattaagaat
      541 ccgaccatta ctagcatacc aaagaaatca gactataaac caaaagctat tgaaagtgag
      601 ggtacggttg attccattac aaccaatgaa atcaatgagt ttttaacgac gttcttcaag
      661 ctctatccta cagcgacagc cagtgaactt tcctactatg tgaatgacgg gatattaaaa
      721 ccaatcggaa aagagtacat ctttcaagaa ctggtaaatc ctatttacaa tcgtaaggat
      781 aatcaagtca cggtatcgct gacagtggag tatatcgacc agcagaccaa agcaacgcag
      841 gtatctcaat ttgatttggt acttgaaaag aacgggagta attggaagat tatagaataa
      901 caaatattgg tacattatta cagctatttt gtaatcacgt actctctttg ataaaaaatt
      961 ggagattcct ttacaaatat gctcttacgt gctattattt aagtatctat ttaaaaggag
     1021 ttaataaata tgcggcaagg tattcttaaa taaactgtca atttgatagt gggaacaaat
     1081 aattggatgt ccttttttag gagggcttag ttttttgcac ccagtttaag aataccttta
     1141 tcatgtgatt ctaaagtatc cggagaatat ctgtatgctt tgtatgccta tggttatgca
     1201 taaaaatccc agtgataaga gtatttatca ctgggatttt tatgcccttt tgggcttttg
     1261 aatggaggaa aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgcggg
     1321 aaaaactacc ttaacagaaa gcttattata taacagtgga gcgattacag aattaggaag
     1381 cgtggacaaa ggtacaacga ggacggataa tacgctttta gaacgtcaga gaggaattac
     1441 aattcagaca ggaataacct cttttcagtg ggaaaatacg aaggtgaaca tcatagacac
     1501 gccaggacat atggatttct tagcagaagt atatcgttca ttatcagttt tagatggggc
     1561 aattctactg atttctgcaa aagatggcgt acaagcacaa actcgtatat tatttcatgc
     1621 acttaggaaa atggggattc ccacaatctt ttttatcaat aagattgacc aaaatggaat
     1681 tgatttatca acggtttatc aggatattaa agagaaactt tctgccgaaa ttgtaatcaa
     1741 acagaaggta gaactgtatc ctaatatgtg tgtgacgaac tttaccgaat ctgaacaatg
     1801 ggatacggta atagagggaa acgatgacct tttagagaaa tatatgtccg gtaaatcatt
     1861 agaagcattg gaactcgaac aagaggaaag cataagattt cagaattgct ccttgtaccc
     1921 tgtttatcat ggaagcgcaa aaagcaacat agggattgag cagcttatag aagtgataac
     1981 gaataaattt tattcatcaa catacagaaa gaagtctgaa ctttgcggaa atgtcttcaa
     2041 aattgaatat tcggaagaaa gacaacgtct tgcatatgta cgcctttatg gcggaatcct
     2101 gcatttgcgg gattcggtta gaatatcgga aaaggaaaaa ataaaaatta cagaaatgta
     2161 tacttcaata aatggtgaat tatgtaaaat tgataaggct tattccgggg aaattgttat
     2221 tttgcaaaat gagtttttga agctaaatag tgttcttgga gatacaaagc tattgccaca
     2281 gagagagaga attgaaaatc cgctccctct gctgcaaaca actgttgaac cgagcaaacc
     2341 tcaacaaagg gaaatgttac ttgatgcact tttagaaatc tccgacagtg acccgcttct
     2401 acaatattat gtggattcta cgacacatga aattatactt tctttcttag ggaaagtaca
     2461 aatggaagtg attagtgcac tgttgcaaga aaagtatcat gtggagatag aactaaaaga
     2521 gcctacagtc atttatatgg agagaccgtt aaaaaatgca gaatatacca ttcacatcga
     2581 agtgccgcca aatcctttct gggcttccat tggtttatct gtatcaccgc ttccgttggg
     2641 aagtggaatg cagtatgaga gctcggtttc tcttggatac ttaaatcaat catttcaaaa
     2701 tgcagttatg gaagggatac gctatggttg cgaacaagga ttatatggtt ggaatgtgac
     2761 ggattgtaaa atctgtttta agtatggctt atactatagc cctgttagta ccccagcaga
     2821 ttttcggatg cttgctccta ttgtattgga acaagtctta aaaaaagctg gaacagaatt
     2881 gttagagcca tatcttagtt ttaaaattta tgcgccacag gaatatcttt cacgagcagg
     2941 ttctgttgca aagttttaaa tctactatca aataagg
//
LOCUS       DACDYJ010000241         2333 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.guided.158, whole genome shotgun sequence.
ACCESSION   DACDYJ010000241 DACDYJ010000000
VERSION     DACDYJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.guided.158"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..304
                     /locus_tag="IWM51_002961"
     CDS             <1..304
                     /locus_tag="IWM51_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972279.1"
                     /translation="GFYSLFKTLQQNPRAYNDAPKYCANIVDTQLKNNEVILSGEIPA
                     RCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            402..1775
                     /gene="tet(L)"
                     /locus_tag="IWM51_002962"
     CDS             402..1775
                     /gene="tet(L)"
                     /locus_tag="IWM51_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP9972280.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT          687 a          355 c          471 g          820 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccca cgagcataca acgatgctcc
       61 taaatattgt gcgaacatcg tagacactca attgaaaaat aatgaggtca ttcttagtgg
      121 agaaatccct gctcggtgta ttcaagaata tcgtagtgat ttaactttct ttacaaatgg
      181 acgtagtgtt tgtttaacag agttaaaagg gtaccatgtt actaccggtg aacctgtttg
      241 ccagccccgt cgtccaaata gtcggataga taaagtacga tatatgttca ataaaataac
      301 ttagtgtatt ttatgttgtt atataaatat ggtttcttgt taaataagat gaaatatttt
      361 ttaataaaga tttgaattaa agtgtaaagg aggaattaat tatgaagtgt aatgaatgta
      421 atagggttaa atacaaccaa attttaattt ggctttgcgt tttatctttt tttagcgtat
      481 taaatgaaat ggttttgaac gtctcattac ctgatattgc aaatgatttt aataaaccac
      541 ctgcgagtac aaactgggtg aacacagcct ttatgttaac cttttccatt ggaacagctg
      601 tatatggaaa gctatctgat caattaggca tcaaaaggtt actcctattt ggaattataa
      661 taaattgttt cgggtcggta attgggtttg ttggccattc tttcttttcc ttacttatta
      721 tggctcgttt tattcaaggg gctggtgcag ctgcatttcc agcactcgta atggttgtag
      781 ttgcgcgcta tattccaaag gaaaataggg gtaaagcatt tggtcttatt ggatcgatag
      841 tagccatggg agaaggagtc ggtccagcga ttggtggaat gatagcccat tatattcatt
      901 ggtcctatct tctactcatt cctatgataa caattatcac tgttccgttt cttatgaaat
      961 tattaaagaa agaagtaagg ataaaaggtc attttgatat caaaggaatt atactaatgt
     1021 ctgtaggcat tgtatttttt atgttgttta caacatcata tagcatttct tttcttatcg
     1081 ttagcgtgct gtcattcctg atatttgtaa aacatatcag gaaagtaaca gatccttttg
     1141 ttgatcccgg attagggaaa aatatacctt ttatgattgg agttctttgt gggggaatta
     1201 tatttggaac agtagcaggg tttgtctcta tggttcctta tatgatgaaa gatgttcacc
     1261 agctaagtac tgccgaaatc ggaagtgtaa ttattttccc tggaacaatg agtgtcatta
     1321 ttttcggcta cattggtggg atacttgttg atagaagagg tcctttatac gtgttaaaca
     1381 tcggagttac atttctttct gttagctttt taactgcttc ctttctttta gaaacaacat
     1441 catggttcat gacaattata atcgtatttg ttttaggtgg gctttcgttc accaaaacag
     1501 ttatatcaac aattgtttca agtagcttga aacagcagga agctggtgct ggaatgagtt
     1561 tgcttaactt taccagcttt ttatcagagg gaacaggtat tgcaattgta ggtggtttat
     1621 tatccatacc cttacttgat caaaggttgt tacctatgga agttgatcag tcaacttatc
     1681 tgtatagtaa tttgttatta cttttttcag gaatcattgt cattagttgg ctggttacct
     1741 tgaatgtata taaacattct caaagggatt tctaaatcgt taagggatca actttgggag
     1801 agagttcaaa attgatcctt tttttataac aggaattcaa atctttttgt tccattaaag
     1861 ggcgcgattg ctgaataaaa gatacgagag acctctcttg tatctttttt attttgagtg
     1921 gttttgtccg ttacactaga aaaccgaaag acaataaaaa ttttattctt gctgagtctg
     1981 gctttcggta agctagacaa aacggacaaa ataaaaattg gcaagggttt aaaggtggag
     2041 attttttgag tgatcttctc aaaaaatact acctgtccct tgctgatttt taaacgagca
     2101 cgagagcaaa accccccttt gctgaggtgg cagagggcag gtttttttgt ttcttttttc
     2161 tcgtaaaaaa aagaaaggtc ttaaaggttt tatggttttg gtcggcactg ccgacagcct
     2221 cgcagagcac acactttatg aatataaagt atagtgtgtt atactttact tggaagtggt
     2281 tgccggaaag agcgaaaatg cctcacattt gtgccaccta aaaaggagcg att
//
LOCUS       DACDYJ010000242         1826 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.guided.172, whole genome shotgun sequence.
ACCESSION   DACDYJ010000242 DACDYJ010000000
VERSION     DACDYJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.guided.172"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..830)
                     /locus_tag="IWM51_002963"
                     /pseudo
     CDS             complement(1..830)
                     /locus_tag="IWM51_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1105..1602
                     /gene="dfrG"
                     /locus_tag="IWM51_002964"
     CDS             1105..1602
                     /gene="dfrG"
                     /locus_tag="IWM51_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9972281.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHTIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          680 a          239 c          325 g          582 t
ORIGIN      
        1 ggctgagaaa ttattttttg attgaaattt tttttactag aaacagttaa agtgattaat
       61 aacaaaacag tcataaataa ataaaatagt tctcttgtct tattgtatct tctatttttg
      121 aaaaatatca tcgttaatat cagagaagag aatattattt gcaagcttaa gatccgccaa
      181 ggaaattgta acaatttggc tggcgtattt tgcagaagat accatggcac caaactagtt
      241 gtagctagcc aagtcaaaca cgtaccataa taaatctttt tttctatgtt tgttaatttt
      301 ttaaataaaa tcaggggaat acataaagat aacagaccaa ctagtcccat tgagtaagta
      361 gttaagtcgt tgttcatagc ttcagtcaga ctatcgaaca cattagatgc atgagcatat
      421 aaatttgtca cgctaggttt gttgattttt tggtagagca tttgttcaaa catcggatac
      481 caaaaagcac tcgtcagaac caatgtcatt cctgccgcac taaataattt tttaaatata
      541 gcaataaaat ctgcattctt gaaaccttta gaaaccaaac gaataaaaat aaaaatgata
      601 attaatgctg tactcattaa tgttgatagt acatggctgt acactagcaa actcatactc
      661 aatgctagct ttacagacgg atacttttct tcttttccag agatgatttg ataagcataa
      721 aataatacag ggataacaag tgttattgcg atagcttcgg caattgcgga acgatagtag
      781 atgtctactg tccgataagc agagaaaata tagagacaac taaataaaaa taagtagttc
      841 agttttggag tacaaaatga caaaatgtac ttcaaaatgg actacttttt tcattttaac
      901 ttacatacca aattacatac aaaatgattt ataaaatgca attgaaaatg ataattgaat
      961 gaaaatttgt gattttgtgg tattatgata atataactta tagaaaaaat tgcttattca
     1021 actaacgggc aggataatga gaaaaggttt tttgatagaa ataagaaatt agatggtaaa
     1081 ttagttaaaa aggctgtgtt ataaatgaaa gtttctttga ttgctgcgat ggataagaat
     1141 agagtgattg gcaaagagaa tgacattcct tggaggattc ccaaggactg ggaatatgtt
     1201 aaaaatacta caaagggaca tacgataata ttaggtagga agaaccttga atcaatcgga
     1261 agagccttac ctgacagaag aaatattatt ctgacgagag ataaggggtt tacctttaat
     1321 ggttgtgaaa ttgttcattc aatagaagat gtttttgagt tatgtaaaaa cgaagaagaa
     1381 atttttattt tcggaggaga acagatttat aatttgtttt tcccttatgt tgagaaaatg
     1441 tacatcacaa aaatacatca tgaattcgaa ggagatactt tttttccaga agtgaattat
     1501 gaggaatgga atgaggtatt tgcccaaaaa gggataaaga atgataaaaa tccgtataac
     1561 tactattttc atgtatatga aagaaaaaac ttattgagtt aacgggtgcg ttaattgaag
     1621 aagaacagta aagataaagc acaagagctt gggattactc caagctcttt ttgtcatttg
     1681 aaataaatta tatctttcca atcaaattgt ttatctgtaa tccctaatct ttgagcaggt
     1741 gtcaaaacct ttttatccgc tgatttaaaa gggcgacaaa agttgtagta agttcttaga
     1801 attgtaatcg caaattgagc atactt
//
LOCUS       DACDYJ010000243         1826 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.guided.173, whole genome shotgun sequence.
ACCESSION   DACDYJ010000243 DACDYJ010000000
VERSION     DACDYJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.guided.173"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..830)
                     /locus_tag="IWM51_002965"
                     /pseudo
     CDS             complement(1..830)
                     /locus_tag="IWM51_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1105..1602
                     /gene="dfrG"
                     /locus_tag="IWM51_002966"
     CDS             1105..1602
                     /gene="dfrG"
                     /locus_tag="IWM51_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9972282.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          679 a          240 c          325 g          582 t
ORIGIN      
        1 ggctgagaaa ttattttttg attgaaattt tttttactag aaacagttaa agtgattaat
       61 aacaaaacag tcataaataa ataaaatagt tctcttgtct tattgtatct tctatttttg
      121 aaaaatatca tcgttaatat cagagaagag aatattattt gcaagcttaa gatccgccaa
      181 ggaaattgta acaatttggc tggcgtattt tgcagaagat accatggcac caaactagtt
      241 gtagctagcc aagtcaaaca cgtaccataa taaatctttt tttctatgtt tgttaatttt
      301 ttaaataaaa tcaggggaat acataaagat aacagaccaa ctagtcccat tgagtaagta
      361 gttaagtcgt tgttcatagc ttcagtcaga ctatcgaaca cattagatgc atgagcatat
      421 aaatttgtca cgctaggttt gttgattttt tggtagagca tttgttcaaa catcggatac
      481 caaaaagcac tcgtcagaac caatgtcatt cctgccgcac taaataattt tttaaatata
      541 gcaataaaat ctgcattctt gaaaccttta gaaaccaaac gaataaaaat aaaaatgata
      601 attaatgctg tactcattaa tgttgatagt acatggctgt acactagcaa actcatactc
      661 aatgctagct ttacagacgg atacttttct tcttttccag agatgatttg ataagcataa
      721 aataatacag ggataacaag tgttattgcg atagcttcgg caattgcgga acgatagtag
      781 atgtctactg tccgataagc agagaaaata tagagacaac taaataaaaa taagtagttc
      841 agttttggag tacaaaatga caaaatgtac ttcaaaatgg actacttttt tcattttaac
      901 ttacatacca aattacatac aaaatgattt ataaaatgca attgaaaatg ataattgaat
      961 gaaaatttgt gattttgtgg tattatgata atataactta tagaaaaaat tgcttattca
     1021 actaacgggc aggataatga gaaaaggttt tttgatagaa ataagaaatt agatggtaaa
     1081 ttagttaaaa aggctgtgtt ataaatgaaa gtttctttga ttgctgcgat ggataagaat
     1141 agagtgattg gcaaagagaa tgacattcct tggaggattc ccaaggactg ggaatatgtt
     1201 aaaaatacta caaagggaca tccgataata ttaggtagga agaaccttga atcaatcgga
     1261 agagccttac ctgacagaag aaatattatt ctgacgagag ataaggggtt tacctttaat
     1321 ggttgtgaaa ttgttcattc aatagaagat gtttttgagt tatgtaaaaa cgaagaagaa
     1381 atttttattt tcggaggaga acagatttat aatttgtttt tcccttatgt tgagaaaatg
     1441 tacatcacaa aaatacatca tgaattcgaa ggagatactt tttttccaga agtgaattat
     1501 gaggaatgga atgaggtatt tgcccaaaaa gggataaaga atgataaaaa tccgtataac
     1561 tactattttc atgtatatga aagaaaaaac ttattgagtt aacgggtgcg ttaattgaag
     1621 aagaacagta aagataaagc acaagagctt gggattactc caagctcttt ttgtcatttg
     1681 aaataaatta tatctttcca atcaaattgt ttatctgtaa tccctaatct ttgagcaggt
     1741 gtcaaaacct ttttatccgc tgatttaaaa gggcgacaaa agttgtagta agttcttaga
     1801 attgtaatcg caaattgagc atactt
//
LOCUS       DACDYJ010000244         1411 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.guided.185, whole genome shotgun sequence.
ACCESSION   DACDYJ010000244 DACDYJ010000000
VERSION     DACDYJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.guided.185"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..245)
                     /locus_tag="IWM51_002967"
     CDS             complement(<1..245)
                     /locus_tag="IWM51_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9972283.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESV"
     gene            798..1346
                     /gene="aac(6')"
                     /locus_tag="IWM51_002968"
     CDS             798..1346
                     /gene="aac(6')"
                     /locus_tag="IWM51_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9972284.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          474 a          229 c          268 g          440 t
ORIGIN      
        1 tggacacttt catgctggat caactgttca atatttccat aagttgaatt acccccgtac
       61 cagagactgg cgacaacctc tatttcctgt gtcaaagcta cttttatttt gttttctgcc
      121 tttgaaagtg cagtcttccc tccaatcagc actacttttg tcccatattc tttgacgatt
      181 ccatttgctt tttcatacgc atctgttcca atggtataat ttggtaagaa aacactttga
      241 ctcatattca tttccttctt tcttcgttga aacgggttaa aattttaaag agtgatcgat
      301 ctgactgcgg ctgtagatgg agtagaggtg aacctaagac aggataagtg atgaataagg
      361 tggcatgaac accttgaaat catcaataag tatcctaaaa acgaacaagt acaaaaacat
      421 tgaaaacgac aataatgaat ttttgttcct cttagtattt ctaccgattc cctttgcttt
      481 tttgtaatta tctaaaaatt ctaaaaatta agattattat atgaaaacag gttttatttg
      541 tcaattcata attggttttt tttaaagcaa aagataatct atatcatgat gaacaaacag
      601 agttttttcc tccaattgat ggctttcctg atctttccta ttttgaataa agtgtttctg
      661 tataattttt ttattttata agtaagataa ataatgaaag aatagagata ctgattggta
      721 gctaaagatt tttgagcgag aacatttcgt tttttcaaaa ggactgttag aataagcaag
      781 aacaaggggg aagacgtatg ataatcagtg aatttgaccg taataatcca gtattgaaag
      841 atcagctttc tgatttactg agactgactt ggccggaaga atatggagac agctcggcag
      901 aagaagtaga agaaatgatg aatccagaac gaatcgcggt agcagcggta gaccaagatg
      961 agttagtagg atttattggt gcaatccctc aatacggtat cacaggttgg gaattgcatc
     1021 cattagttgt agaaagctcc cgacgaaaga accaaatagg tactcgatta gtcaattact
     1081 tagaaaaaga agtagcttcc agaggaggaa tcacgattta tttaggtacg gatgatttag
     1141 accatggaac aacgttaagt caaacggacc tgtatgagca tacatttgat aaagtggctt
     1201 ccatccagaa ccttcgtgaa catccgtatg aattctatga aaaattaggt tataaaatcg
     1261 taggtgtctt accaaatgca aatggctggg acaaaccgga tatttggatg gcaaaaacga
     1321 ttattcctcg accaaattct caataataaa aagaggttgt gacagaagta atcagcttca
     1381 agaaataaga aggaatttac gaaaattact t
//
LOCUS       DACDYJ010000245         1328 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.guided.189, whole genome shotgun sequence.
ACCESSION   DACDYJ010000245 DACDYJ010000000
VERSION     DACDYJ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.guided.189"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            37..>1328
                     /gene="aac(6')-Ie"
                     /locus_tag="IWM51_002969"
     CDS             37..>1328
                     /gene="aac(6')-Ie"
                     /locus_tag="IWM51_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HAP9972285.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYG"
BASE COUNT          554 a           98 c          232 g          444 t
ORIGIN      
        1 gtatatgtat atgattatga aaaaggtgat aaataaatga atatagttga aaatgaaata
       61 tgtataagaa ctttaataga tgatgatttt cctttgatgt taaaatggtt aactgatgaa
      121 agagtattag aattttatgg tggtagagat aaaaaatata cattagaatc attaaaaaaa
      181 cattatacag agccttggga agatgaagtt tttagagtaa ttattgaata taacaatgtt
      241 cctattggat atggacaaat atataaaatg tatgatgagt tatatactga ttatcattat
      301 ccaaaaactg atgagatagt ctatggtatg gatcaattta taggagagcc aaattattgg
      361 agtaaaggaa ttggtacaag atatattaaa ttgatttttg aatttttgaa aaaagaaaga
      421 aatgctaatg cagttatttt agaccctcat aaaaataatc caagagcaat aagggcatac
      481 caaaaatctg gttttagaat tattgaagat ttgccagaac atgaattaca cgagggcaaa
      541 aaagaagatt gttatttaat ggaatataga tatgatgata atgccacaaa tgttaaggca
      601 atgaaatatt taattgagca ttactttgat aatttcaaag tagatagtat tgaaataatc
      661 ggtagtggtt atgatagtgt ggcatattta gttaataatg aatacatttt taaaacaaaa
      721 tttagtacta ataagaaaaa aggttatgca aaagaaaaag caatatataa ttttttaaat
      781 acaaatttag aaactaatgt aaaaattcct aatattgaat attcgtatat tagtgatgaa
      841 ttatctatac taggttataa agaaattaaa ggaacttttt taacaccaga aatttattct
      901 actatgtcag aagaagaaca aaatttgtta aaacgagata ttgccagttt tttaagacaa
      961 atgcacggtt tagattatac agatattagt gaatgtacta ttgataataa acaaaatgta
     1021 ttagaagagt atatattgtt gcgtgaaact atttataatg atttaactga tatagaaaaa
     1081 gattatatag aaagttttat ggaaagacta aatgcaacaa cagtttttga gggtaaaaag
     1141 tgtttatgcc ataatgattt tagttgtaat catctattgt tagatggcaa taatagatta
     1201 actggaataa ttgattttgg agattctgga attatagatg aatattgtga ttttatatac
     1261 ttacttgaag atagtgaaga agaaatagga acaaattttg gagaagatat attaagaatg
     1321 tatggaaa
//
LOCUS       DACDYJ010000246         1091 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.guided.200, whole genome shotgun sequence.
ACCESSION   DACDYJ010000246 DACDYJ010000000
VERSION     DACDYJ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.guided.200"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..64
                     /locus_tag="IWM51_002970"
     CDS             <1..64
                     /locus_tag="IWM51_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP9972286.1"
                     /translation="YVDKTSTVLKQTKNSDYADK"
     gene            189..926
                     /gene="erm(B)"
                     /locus_tag="IWM51_002971"
     CDS             189..926
                     /gene="erm(B)"
                     /locus_tag="IWM51_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9972287.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          417 a          181 c          161 g          332 t
ORIGIN      
        1 ttatgtagat aaaacatcta ctgttttgaa acagactaaa aacagtgatt acgcagataa
       61 ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta aacagataac
      121 taaaattaca aacaaatcgt ttaacttctg tatttgttta tagatgtaat cacttcagga
      181 gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt gaaaaagtac
      241 tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac gaaattggaa
      301 caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta acgtctattg
      361 aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaat actcgtgtca
      421 ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg tataaaattg
      481 ttgggagtat tccttaccat ttaagcacac aaattattaa aaaagtggtt tttgaaagcc
      541 atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc ttggatattc
      601 accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg cttaagctgc
      661 cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa cttacccgcc
      721 ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt tcaaaatggg
      781 tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca atgaaacacg
      841 ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt tttaatagtt
      901 atctattatt taacgggagg aaataattct atgagtcgct tttgtaaatt tggaaagtta
      961 cacgttacta aagggaatgt agataaatta ttaggtatag ggcacctcta ataactacca
     1021 attaattcac ctcaaaatga aattagaaaa aaacagcgaa atttttaaat ctatttctta
     1081 tcgatacaaa t
//
LOCUS       DACDYJ010000247         1043 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31907_2017
            SAMN12639881-rid9881233.guided.204, whole genome shotgun sequence.
ACCESSION   DACDYJ010000247 DACDYJ010000000
VERSION     DACDYJ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639881
            Sequence Read Archive: SRR10023366
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741891.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 05:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,839
            CDSs (with protein)               :: 2,839
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 31 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639881-rid9881233.guided.204"
                     /isolate="E31907_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            86..145
                     /gene="ermCL"
                     /locus_tag="IWM51_002972"
     CDS             86..145
                     /gene="ermCL"
                     /locus_tag="IWM51_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HAP9972288.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            205..939
                     /locus_tag="IWM51_002973"
     CDS             205..939
                     /locus_tag="IWM51_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HAP9972289.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          419 a          138 c          140 g          346 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccag cttgtgctat aattattcta
       61 atttcataaa aaggagaatt aatatatggg catttttagt atttttgtaa tcaacacagt
      121 tcattatcaa ccaaacaaaa aataagtggt tataatggat tgttaatata aacattcatt
      181 ataacctcat aggagtgggt tataatgaac aaaaaaaata taaaagatag tcaaaacttt
      241 attacttcaa agcatcatat aaatgaaatt ttgagaaatg tacatttaaa tacaaatgat
      301 aatattattg agattggttc agggaaaggt catttcacgt ttgaattagc taaaaggtgt
      361 aattatgtaa ccgccattga aatagatcct aaattatgta ggataactaa aaacaaactt
      421 attgaatatg agaacttcca ggttatcaat aaagatattt tacaatttaa gtttcctaaa
      481 aataagtcat ataagatata tggaaatata ccctacaata taagtacaga tataattcga
      541 aaaattgttt ttgaaagcac agctacagaa agttatttaa tagtggaata tggatttgct
      601 aaaaggttgc taaatacaaa tcgttcacta gcactatttt taatgacaga agttgatata
      661 tccatattaa gtaaaatccc tagagaatac tttcatccaa aacctagagt taatagctcg
      721 ttaattgtat taaaaagaca cccttcaaaa atatcactca aagatagaaa acaatatgaa
      781 aattttgtta tgaaatgggt taacaaagaa tacataaaac tattttccaa aaaccaattt
      841 tatcaagcct taaaatatgc aagaattgac gatttaaaca atattagctt tgaacaattc
      901 ttgtctcttt tcaatagcta taaattattt aatagataag ttaaggggtg cataaactgc
      961 atcccttaac ttatcttatt ttgcttaaac aatcttggcc cgtaaactgc ataactcata
     1021 tgtaaatcgc tcctttttag gtg
//
